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From: zaluzec-at-microscopy.com
Date: Fri, 3 Jan 2020 08:22:59 -0600
Subject: [Microscopy] Happy New Year 2020

Contents Retrieved from Microscopy Listserver Archives
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Happy New Year Colleagues;

Welcome to the another year of operation of the Microscopy ListServer
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From: gary_laevsky-at-yahoo.com
Date: Mon, 6 Jan 2020 07:51:12 -0600
Subject: [Microscopy] Big Data, Big Problems; Light-sheet workshop Princeton University,

Contents Retrieved from Microscopy Listserver Archives
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Email: mlynn-at-ameslab.gov Name: Matthew Lynn

Organization: Ames Laboratory, Dept. of Energy

Title-Subject: [Filtered] TEM: Postdoctoral position at the Ames Laboratory

Message: The Division of Materials Science and Engineering (DMSE) at the Ames Laboratory, a
Department of Energy National Laboratory affiliated with Iowa State University, is searching for a
qualified Postdoctoral Research Associate.

This is an opportunity to support a diverse range of research programs using advanced
aberration-corrected STEM and associated techniques.
More details and application instructions can be found here:

https://isu.wd1.myworkdayjobs.com/en-US/IowaStateJobs/job/Ames-IA/Postdoc-Research-Associate_R444



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From fadecice330ojoi-at-gmail.com Mon Jan 6 07:32:29 2020
Return-Path: {fadecice330ojoi-at-gmail.com}
Received: from gmail.com (a3.68474.cn [23.228.73.183] (may be forged))
by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 006DWSaq013173
for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 6 Jan 2020 07:32:28 -0600
Message-ID: {029F40E5.7510914E-at-gmail.com}

Dear Colleagues,


It's the beginning of the new year and it's time to plane your future training opportunities!


We are excited to be offering the first, of what promises to be annual, Big Data, Big Problems light-sheet workshop, held at Princeton University, Princeton, NJ, USA.  The course will run July 27-July 31, 2020.


Applications are due April 15, 2020.  Apply through the course web site at BigDataBigProblems.com.


Multi-dimensional live and fixed microscope data can be collected in so many ways, and the various solutions seem to grow almost daily.  There are many courses that focus on general optical principals and the use of conventional microscope platforms such as widefield fluorescence, confocal and multiphoton imaging, but none that are specifically designed to demystify rapidly evolving and increasingly prescient methods such as light-sheet imaging.  Ultimately this is the goal of this course, what is light-sheet microscopy in all its flavors, what can you do with it, how do you choose between platforms and once you have a system or are proficient with a device what do you do with the data?  This week long course brings together experts in conventional optics, all aspects of light sheet microscopy and image analysis to help you bring your light-sheet based cellular, animal and large sample/cleared sample imaging to the next level.  From the syllabus you will see that we start out with principals and choices and then move into specific systems instructed by both academic and industry faculty.  In fact, we have will have almost all the available commercial solutions on site for you to use.  We will provide samples, but equally you are welcome to use your own.   Our goal is that you will return to your home institution fully capable to implement and use these truly exciting new tools.


Course Speakers;

Amy Elliot National Institutes of Health

Holly Gibbs Texas A&M

Elizabeth Hillman Columbia University

Jan Huisken Morgridge Institute for Research

Gary Laevsky Princeton University

Talley Lambert Harvard University

Wesley Legant University of North Carolina

Paul Maddox University of North Carolina

Alison North The Rockefeller University

Eszter Posfai Princeton University

Doug Richardson Harvard University

Kelly Seagraves Princeton University

Hari Shroff National Institutes of Health

Claudette St. Croix University of Pittsburgh

Sebastian Streichan University of California Santa Barbara

Jared Toettcher Princeton University

Simon Watkins University of Pittsburgh

 
Applications are due April 15, 2020.  Apply through the course website, BigDataBigProblems.com

 
Course Directors

Gary Laevsky, Princeton University

Simon Watkins, University of Pittsburgh



--

Best,


Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Co-Founder, North Atlantic Microscopy Society (NAMS) 
https://namsmicroscopy.com/
Dept. of Molecular Biology
Washington Rd.
Princeton University 
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310


North Atlantic Microscopy Society Spring Meeting at UPENN, April 23, 2020.


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41, 37 -- Date: Mon, 6 Jan 2020 12:48:19 +0000 (UTC)
41, 37 -- From: Gary Laevsky {gary_laevsky-at-yahoo.com}
41, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
41, 37 -- Message-ID: {1153505689.4734631.1578314899793-at-mail.yahoo.com}
41, 37 -- Subject: Big Data, Big Problems; Light-sheet workshop Princeton University,
41, 37 -- July 27-31, 2020
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From: microscopy.listserver-at-gmail.com
Date: Mon, 6 Jan 2020 10:07:49 -0600
Subject: [Microscopy] viaWWW: question about fib milling parameters on polymer membrane

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

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Email: helene_roberge-at-hotmail.fr Name: hÈlËne Roberge

Organization: imn

Title-Subject: [Filtered] question about fib milling parameters on polymer membrane

Message: Dear Colleagues,

First at all, Happy New Year !

then, I have a question for you :
i started my phd in last october and i work on polymer membrane. My goal is to image, in a fist
time, the 3D structure of my membrane. For this, i want to use the FIB/SEM available in my lab. I
know this is fragile sample and i need to adapt my parameters for milling my surface without damage
them.

This is my question :
Someone alrady use this technique on polymer membrane ? And have an idea of optimal parameters
(current, energy, deep, dual time..) for use the ion beam ?
I have PES and PAN membranes. I need reference parameters for this type of sample.

Thanks a lot and have a nice day

Best regards

HÈlËne Roberge
Phd student
IMN - Institut des MatÈriaux Jean Rouxel
2, rue de la HoussiniËre - BP 32229
44 322 NANTES CEDEX 3
https://www.cnrs-imn.fr/
0240376316


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From: gary_laevsky-at-yahoo.com
Date: Mon, 6 Jan 2020 12:06:26 -0600
Subject: [Microscopy] North Atlantic Microscopy Society (NAMS) Spring Meeting; April 23,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

I’m excited to announce that I’ll be hosting the spring 2020 meeting of the North Atlantic Microscopy Society (NAMS) here at the Perelman School of Medicine at the University of Pennsylvania on April 23rd.

NAMS was founded by Gary Laevsky and Paul Shao at Princeton University. Its mission is to promote microscopy education and to provide researchers and vendors in the region with opportunities to connect and hear about the latest applications of microscopy techniques. 

The focus of this meeting will be Correlative Light-Electron Microscopy (CLEM). Light and electron microscopy (EM) speakers will highlight their respective techniques, while CLEM speakers will demonstrate the benefits of bringing the two modalities together.

8:15 - 9:00 am: Registration and coffee
9:00 - 9:20:            Introductory remarks
9:30 - 10:15:           Vera Moiseenkova-Bell (UPenn - Cryo EM)
10:15 - 11:00:          Tim Mosca (Thomas Jefferson University - light microscopy)
11:00 - 11:30:          Break
11:30 - 12:30 pm:       Tech Bites (5-minute lightning talks by vendors)
12:30 - 2:00:           Lunch/Vendors
2:00 - 3:00:            Tatyana Svitkina (UPenn - CLEM)
3:00 - 4:00:            Poster Session/Vendors (snacks & refreshments provided)
4:00 - 5:00:            Justin Taraska (NIH - CLEM)

You can register for the meeting here: https://namsmicroscopy.com/meeting-registration

I hope to see some of you here in April!

Andrea

Andrea L. Stout, Ph. D.
Director,  CDB Microscopy Core Facility
Perelman School of Medicine at the University of Pennsylvania
1107 BRB 2/3
421 Curie Boulevard
Philadelphia, PA 19104

web: http://www.med.upenn.edu/cdbmicroscopycore/
Twitter: -at-CDBMicroCore


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From: microscopy.listserver-at-gmail.com
Date: Mon, 6 Jan 2020 18:57:21 -0600
Subject: [Microscopy] viaWWW:Reminder EGU 2020: Advances in microanalysis: Insights into

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Email: renelle.dubosq-at-gmail.com Name: Renelle Dubosq

Organization: University of Ottawa

Title-Subject: [Filtered] Reminder EGU 2020: Advances in microanalysis: Insights into nanoscale
trace element heterogeneities

Message: Dear colleagues,

We would like to invite submissions of abstracts to our EGU 2020 session applying advanced
microanalysis techniques to investigate chemical heterogeneities.

GMPV1.3 "Advances in microanalysis: Insights into nanoscale trace element heterogeneities"
Convener: Renelle DubosqECS | Co-conveners: Tyler BlumECS, Sandra Piazolo

Invited speakers:
Dr. Desmond Moser, Western University, will present on trace elements and microstructures from Early
Mars and the geochronology of habitability

Dr. Ana Cernok, Royal Ontario Museum, will present on shock‐induced microtextures in lunar
apatite and merrillite

Please follow this link: https://meetingorganizer.copernicus.org/EGU2020/session/35196 for a full
description of the session.

Abstract submission (deadline: 15 January 2020, 13:00 CET)

Looking forward to seeing you in Vienna,
Best regards,

Renelle, Tyler and Sandra

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From: microscopy.listserver-at-gmail.com
Date: Mon, 6 Jan 2020 18:57:51 -0600
Subject: [Microscopy] viaWWW:Biological TEM Workshop, March 11

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Email: johnshields59-at-gmail.com Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] Biological TEM Workshop, March 11

Message: *Biological TEM Workshop*
This intensive, three-day workshop provides a practical and basic theoretical introduction to the
Transmission Electron Microscope and biological sample preparation techniques. Each day will consist
of lecture, discussion and *hands-on* training led by GEM staff.
Who: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment.
When: Wednesday through Friday, March 11-13 2020, 8am-5pm each day (lunch is provided)
Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login
Deadline: March 4, 2020


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From: microscopy.listserver-at-gmail.com
Date: Mon, 6 Jan 2020 18:58:35 -0600
Subject: [Microscopy] viaWWW: jeol JSM 6360 SEM error codes

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Title-Subject: [Filtered] jeol JSM 6360 SEM

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From: diller-at-stefan-diller.com
Date: Wed, 8 Jan 2020 05:17:36 -0600
Subject: [Microscopy] Activated Carbon - how to measure active surface

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

one of my customers is producing active carbon, I suppose, from different kind of wood.

Is there any (SEM microscopic) way to find out the active surface value in square meters per gram of carbon? In a more precise way
than to estimate it?


Thanks, and happy new year

Stefan


--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


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12, 28 -- Subject: Activated Carbon - how to measure active surface
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From: wesaia-at-iastate.edu
Date: Wed, 8 Jan 2020 11:05:59 -0600
Subject: [Microscopy] Activated Carbon - how to measure active surface

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

In a word, no.

My understanding is that the pore structure would push the limit of SEM to characterize it. I don't think there would be any reliable way to correlate the microstructure with an estimate of specific surface area. And if the operator or SEM was having an off day, they would fail to resolve the porosity and thus fail to properly compare samples.

I recall that BET is the norm for measuring specific surface. I presume it works for activated carbon. I think it would give much more reliable number than anything that could be done microscopically.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
X-from: diller-at-stefan-diller.com {diller-at-stefan-diller.com}
Sent: Wednesday, January 08, 2020 5:18 AM
To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

Dear All,

one of my customers is producing active carbon, I suppose, from different kind of wood.

Is there any (SEM microscopic) way to find out the active surface value in square meters per gram of carbon? In a more precise way than to estimate it?


Thanks, and happy new year

Stefan


--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


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12, 28 -- Subject: Activated Carbon - how to measure active surface
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From: owenha-at-uwm.edu
Date: Fri, 10 Jan 2020 09:54:25 -0600
Subject: [Microscopy] TEM - Ultramicrotome Light for RMC MT-7000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

Apologies for the double post, but the deadline for our advertised
3-year postdoc position in STEM imaging at Monash University has been
extended to Friday 24th January. If you know anyone interested who may
not have been able to apply over the holiday period, there's still time.

Full details at:
http://careers.pageuppeople.com/513/cw/en/job/600527/research-fellow-in-atomicscale-structure-determination-in-thick-nanostructures

Kind regards,
Scott


On 22/11/2019 8:08 am, Scott Findlay wrote:
} Dear colleagues,
}
} I'm advertising a 3-year postdoc position in atomic-scale structure
} determination in thick nanostructures at the School of Physics and
} Astronomy, Monash University, Australia.  Further details below.
}
} Please bring this to the attention of anyone who may be interested.
}
} Many thanks,
} Scott Findlay
}
} ___________________
}
} Position Descriptions and application details at:
} http://careers.pageuppeople.com/513/cw/en/job/600527/research-fellow-in-atomicscale-structure-determination-in-thick-nanostructures
}
}
} Position overview: The Research Fellow will work on developing methods
} for atomic-scale structure determination via scanning transmission
} electron microscopy. This project aims to develop a theoretical and
} computational toolkit for structure retrieval at atomic resolution that
} is robust in the presence of multiple scattering (“dynamical
} diffraction”) of the electron probe, and to apply it to large
} experimental data sets obtained from the new generation of fast-readout
} electron detectors. The project may draw on methods from inverse
} scattering theory, phase retrieval, iterative algorithms, machine
} learning, and high-performance computing.
}
} Duration: 3-year fixed-term appointment
} Remuneration package: $68,040 - $92,343 pa Level A (plus 17% employer
} superannuation)
} Closing date: Friday 10th January, 2020

--
*SCOTT FINDLAY*
Senior Lecturer

*Monash University*
School of Physics and Astronomy
G08, New Horizons Centre, 20 Research Way, Clayton Campus
Clayton, VIC 3800
Australia

T: +61 3 9902 4943
E: scott.findlay-at-monash.edu {mailto:scott.findlay-at-monash.edu}
monash.edu {http://monash.edu}

==============================Original Headers==============================
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From normanatlas54ykur-at-gmail.com Wed Jan 8 16:53:37 2020
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Hi Everyone,

We are in the midst of refurbishing an RMC MT-7000 ultramicrotome to replace our MT-7 teaching ultramicrotome that has developed a problem we haven't been able to fix. Unfortunately, the instrument I acquired is missing the horizontal overhead light (but luckily, not the mounting bar). RMC has been fantastic in support for this project, but doesn't have a light available that I can purchase. We've thought about modifying the light from the MT-7, but I still have hopes for getting it going again.

If anyone should happen to have an out of service MT-7000 and would be willing to part with the light fixture, curly cord and/or the plug for the light, please let me know. I have images of the overhead light available that I can send by regular email. The lamp from our other MT-7000 works perfectly with the unit under construction, so no worries about the rest of the circuit.

Thanks for any help,
Heather

Heather A. Owen, Ph.D.
Director, Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
3209 N. Maryland Ave.
Milwaukee, WI† 53211

(414)229-6816

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6, 70 -- From: Heather A Owen {owenha-at-uwm.edu}
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6, 70 -- Subject: TEM - Ultramicrotome Light for RMC MT-7000
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From: Louis.Gambino-at-jmusa.com
Date: Fri, 10 Jan 2020 14:45:41 -0600
Subject: [Microscopy] Job Posting for Scientist II - Electron Microscopy

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Job Location: Johnson Matthey, Clean Air, Wayne, PA, USA

Job Description: The Development Analytical Services (DAS) Scientist II takes day-to-day responsibility for OM and SEM instrumentation and provides support to R&D projects that generate new products, processes and understanding of commercial value to JM while building an understanding of the science involved. The incumbent will perform routine analytical analyses using the scanning electron microscopy and a significant amount of non-routine work using existing analytical procedures that serve to characterize the properties, function, or composition of catalytic materials or materials of catalytic interest. The incumbent supports JM through all aspects of catalytical material discovery and delivery including analytical analysis, liaise with internal customers, and support of JM processes and practices. Individual will have responsibility for managing projects from inception to close.

For more details and how to apply: {https://chu.tbe.taleo.net/chu01/ats/careers/requisition.jsp?org=JOHNSONMATTHEY&cws=1&rid=9712} .

Louis Gambino, PhD
Associate Scientist
Johnson Matthey
436 Devon Park Drive
Wayne, PA 19087-1816
If the reader of this email is not the intended recipient(s), please be advised that any dissemination, distribution or copying of this information is strictly prohibited. Johnson Matthey Inc. has its main place of business at 435 Devon Park Drive, Wayne, PA, 19087 USA. While Johnson Matthey aims to keep its network free from viruses you should note that we are unable to scan certain emails, particularly if any part is encrypted or password-protected, and accordingly you are strongly advised to check this email and any attachments for viruses. The company shall NOT ACCEPT any liability with regard to computer viruses transferred by way of email. Please note that your communication may be monitored in accordance with Johnson Matthey internal policy documentation.


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From: microscopy.listserver-at-gmail.com
Date: Sat, 11 Jan 2020 08:38:16 -0600
Subject: [Microscopy] viaWWW:GWNIC CLEM WorkshopJune 8-12, 2020

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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

Organization: The George Washington University

Title-Subject: [Filtered] GWNIC CLEM WorkshopJune 8-12, 2020

Message: Greeting Microscopy Listers

The Nanofabrication and Imaging Center at George Washington University will be holding its 4th
annual CLEM workshop June 8-12, 2020 in Washington DC. If you are interested in applications and
workflow for CLEM projects, then this workshop is for you.

Please visit the website for further details.

https://nic.gwu.edu/clem-workshop

Happy New Year
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From: microscopy.listserver-at-gmail.com
Date: Sat, 11 Jan 2020 08:43:08 -0600
Subject: [Microscopy] viaWWW:Upcoming Microscopy Workshops at EMS Microscopy Academy

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Organization: EMS Acquisition Corp

Title-Subject: [Filtered] Upcoming Microscopy Workshops at EMS Microscopy Academy

Message: Materials Ultramicrotomy
February 24, 2020 For those unfamiliar with microtomy to prepare for the workshop.
February 25 - 27, 2020
This workshop will introduce individuals to the unique application of ultramicrotomy to materials.
Learn more at https://www.emsmicroscopyacademy.com/product-page/materials-feb20
Aurion Immunogold Silver Staining
April 15 - 17, 2020
Three days of hands-on training for students, researchers, and microscopists who want to learn
the most up to date theory and practice in immunogold labeling.
Learn more at https://www.emsmicroscopyacademy.com/product-page/immunogold-apr20
Cryosectioning/Immunogold
April 20 - 24, 2020
Hands-on training for students, researchers, and microscopists who want to learn the most up to
date theory and practice in cryosectioning and immunogold labeling.
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From: diller-at-stefan-diller.com
Date: Sun, 12 Jan 2020 14:09:44 -0600
Subject: [Microscopy] Re: Activated Carbon - how to measure active surface -

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

thanks for the replies to this topic. I suppose my customer will do it the chemical way...

But it had been nice to experience some insights in this peculiar topic ;-)


Stefan



}
}
}
}
}
} -----Original Message-----
} From: diller-at-stefan-diller.com {diller-at-stefan-diller.com}
} Sent: Thursday, 9 January 2020 12:24 AM
} To: Harland, Duane {Duane.Harland-at-agresearch.co.nz}
} Subject: [Microscopy] Activated Carbon - how to measure active surface
}
}
}
}
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} Dear All,
}
} one of my customers is producing active carbon, I suppose, from different kind of wood.
}
} Is there any (SEM microscopic) way to find out the active surface value in square meters per gram of carbon? In a more precise way than to estimate it?
}
}
} Thanks, and happy new year
}
} Stefan
}
}
} --
}
}
} -----------------------------------------------------
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From: microscopy.listserver-at-gmail.com
Date: Mon, 13 Jan 2020 21:36:58 -0600
Subject: [Microscopy] viaWWW:position available - Applications Specialist in FIB-SEM Tescan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello and happy New Year to all,

The Analytical Instrumentation Facility (AIF) and Research Triangle
Nanotechnology Network (RTNN) at North Carolina State University, in
partnership with Protochips and ThermoFisher Scientific, are pleased
to announce the first annual In Situ Microscopy Congress (ISMC). The
meeting will be held at North Carolina State University in Raleigh, NC
on March 2nd and 3rd, 2020.

This is a two day workshop which provides one the opportunity to learn
about liquid phase and gaseous phase in situ capabilities, and
includes two plenary talks from leaders in the respective fields along
with hands-on demonstrations of liquid and gas cell holders and our
Talos F200X, probe-corrected Titan, and Quanta 3D FIB/SEM platforms.

This workshop is open to all. Attendees are encouraged to submit an
abstract to be considered for an oral or poster presentation. To
register, or for more information, please visit the following
registration link:

https://www.eventbrite.com/e/in-situ-microscopy-congress-tickets-83202188987

We look forward to seeing you,
Chris
--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
https://www.aif.ncsu.edu/
Cell: 267-496-0587

==============================Original Headers==============================
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Email: tescanmicroscopy-at-gmail.com Name: dj miller

Organization: Tescan USA

Title-Subject: [Filtered] position available - Applications Specialist in FIB-SEM Tescan USA

Message: TESCAN USA, a fast-growing solution provider in electron, ion and x-ray CT in the
microscope business, is looking for an Applications Specialist in FIB-SEM. This position is
responsible for supporting the sales process of Tescan FIB-SEMs through tool demonstrations, sample
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Microscopy and intimate familiarity with FIB-SEM tools and techniques such as TEM sample
Preparation, 3D cross-sectioning and reconstruction (image, EDS & EBSD), delayering, nano-probing,
circuit edit, lithography and other. Experience in other techniques such as TOF-SIMS, cryo sample
preparation, APT, TEM would be a plus. Excellent communication in English is required. This
position located in the Pleasanton, CA, but will require travel up to 50% of the time.

TESCAN USA will only employ individuals who are legally authorized to work in the United States for
this opening (i.e. ñ no sponsorship is available). Any offer of employment may be conditional upon
the successful completion of a background investigation and drug screening.
TESCAN USA offers competitive salaries and benefits.
Qualified candidates should send their resume, cover letter and salary requirements to:
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From: microscopy.listserver-at-gmail.com
Date: Mon, 13 Jan 2020 21:37:40 -0600
Subject: [Microscopy] viaWWW:Staff position CLEM and Bio EM at Monash University,

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Email: georg.ramm-at-monash.edu Name: Georg Ramm

Organization: Monash Ramaciotti Centre
Title-Subject: [Filtered] Staff position CLEM and Bio EM at Monash University, Melbourne, Australia

Message: We are looking for a staff member with expertise in CLEM to join our team at the Monash
Ramaciotti Centre at Monash University (https://www.monash.edu/researchinfrastructure/cryo-em)
Senior Research Officer - Correlative Light and Electron Microscopy
Location: Clayton campus, Melbourne, Australia

Employment Type: Full-time, 3 year Fixed-term appointment

Remuneration: $98,155 - $108,345 pa HEW Level 08 (plus 17% employer superannuation)

Contact: Georg Ramm, Georg.ramm-at-monash.edu, +61-3 - 9905 1280

Closing date: 31 January 2020
Link to job advertisement:
https://careers.pageuppeople.com/513/cw/en/job/599948/senior-research-officer-correlative-light-and-electron-microscopy

As the successful candidate you will:

Manage, plan, coordinate and oversee EM projects in collaboration with users Develop new protocols
for correlative light and electron microscopy and life sciences EM techniques and apply them to
research projects
Teach specialised EM techniques such as CLEM and immuno EM to Monash researchers and to the wider
Australian and international EM community
Perform microscopy and related EM techniques including immuno EM, (cryo-) ultramicrotomy, high
pressure freezing, and cryo-preparation

The Monash Ramaciotti Centre is a leading facility for life sciences electron microscopy. It houses
Australiaís first Titan Krios microscope, a cryo-FIBSEM Helios G4 with Leica VCT500 cryo-stage, a
Talos Arctica, as well as two 120keV TEMs and a FESEM. A suite of advanced sample preparation and
other equipment is available, including a Zeiss LSM900 Airyscan with Linkam cryo-stage, a Wohlwend
high pressure freezer, Leica AFS2 and FC7 cryo-ultramicroscopes. The facilityís expert team supports
and collaborates on a large number of bio EM techniques ranging from standard SEM and TEM to immuno
EM, correlative light and electron microscopy, cryotomography and single particle analysis.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 15 Jan 2020 07:46:58 -0600
Subject: [Microscopy] viaWWW:Light Leakage from Microscope into Digital Camera

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Email: bowmanpond-at-gmail.com Name: Mike Codner

Title-Subject: [Filtered] Light Leakage from Microscope into Digital Camera

Message: While familiarizing myself with a newly purchased Swiftcam 18 MP Microscope Camera, I was
having a problem mating it to my LW Scientific Revelation III Binocular Microscope using Swift's 3.0
software and Windows 10. Upon viewing pond water specimens, the background lighting kept changing
from white to darker colors and vice-versa. I had made all the adjustments the manual had
recommended including exposure, white balance, color settings, and the like. Nothing worked. To
make a long story short, the problem resolved itself when I plugged the non-camera binocular with a
black rubber stopper after removing the lens. Apparently there had been light leakage from this
binocular into the one containing the camera. Just wanted to alert your members since this problem
was very frustrating and I could find no helpful information either in the manual or online.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 15 Jan 2020 12:52:38 -0600
Subject: [Microscopy] viaWWW Post Doctoral Position - Developing a Cryo-compatible Specimen

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Email: sichen-at-anl.gov

Name: Si Chen

Organization: Argonne National Laboratory

Title-Subject: [Filtered] Post Doctoral Position - Developing a Cryo-compatible Specimen Prep
workflow for Xray and Electron Microscopy -at- Argonne National Laboratory

Message: Position Description

The X-ray Science Division (XSD) of Advanced Photon Source (APS) at Argonne National Laboratory is
looking for a Postdoctoral Researcher who will be responsible for developing novel apparatus and
methods to enable multi-scale and multi-modality analysis of materials via both electron microscopy
and X-ray microscopy at the synchrotron facility. The research aims to deliver a cryo-compatible
workflow and supporting techniques to optimize sample preparation for X-ray fluorescence nanoprobe
as well as the combined use of multiple analytical platforms. It will also involve software
development for image registration across different analytical platforms and correlative data
analysis. The successful candidate will work in a multidisciplinary team environment including
physicists, chemists, biologists and computer scientist. The candidate will lead the effort in
developing the methodology, the deployment at the APS beamlines, as well as applications to
materials, biological and environmental sciences. Results will be reported in appropriate forms:
publications in refereed journals and oral presentations at meetings, conferences, and seminars. The
position will begin in April 2020. It is a one-year position, and renewable for a second year.

Position Requirements

*Ph.D. degree in physics, materials science, engineering, or a related discipline;
*Comprehensive knowledge in at least two of the following fields: X-ray physics, X-ray microscopy,
electron microscopy, image processing;
*Considerable experience with electron microscopy, focused ion beam, synchrotron facility,
preferably cryogenic instruments;
*Strong data analysis and trouble-shooting skills;
*Considerable experience with programming and software tools such as python and git;
*Experience with automation, control, and instrument infrastructure is a plus;
*Knowledge of biology, chemistry is a plus;
*Experience with cryogenic is a plus;
*Demonstrated experience working successfully as part of a team in a collaborative and
multidisciplinary scientific environment;
*Ability to communicate effectively with internal and external collaborators and ability to work in
a team environment.

Additional Details and Application Forms can be found at this URL

https://careers.peopleclick.com/careerscp/client_argonnelab/post_doc/jobDetails.do?functionName=getJobDetail&jobPostId=8696&localeCode=en-us

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14, 54 -- Subject: viaWWW Post Doctoral Position - Developing a Cryo-compatible Specimen
14, 54 -- Prep workflow for Xray and Electron Microscopy -at- Argonne National Laboratory
14, 54 -- References: {202001151822.00FIMoP5026403-at-microscopy.com}
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From: microscopy.listserver-at-gmail.com
Date: Thu, 16 Jan 2020 19:16:39 -0600
Subject: [Microscopy] viaWWW: Syllabus/Lectures for Cellular & Molecular Electron

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Email: whiteto-at-missouri.edu Name: Tommi White

Organization: University of Missouri Electron Microscopy Core

Title-Subject: [Filtered] Cellular & Molecular Electron Microscopy

Message: Hello Microscopy Listserve,

I am taking over a graduate level class entitled "Cellular & Molecular Electron Microscopy" from a
retiring faculty member. Would any of you be so kind to share syllabus/lecture materials that I
could use in this course? Of course, full credit will be given to the provider of the information!
:) Thanks in advance to helping educate the next generation of electron microscopists and light
that fire.
Tommi
Tommi A. White, Ph.D.
Director, Electron Microscopy Core
Assistant Research Professor, Biochemistry
University of Missouri
Mail: W117 Veterinary Medicine Bldg
1520 E Rollins St., Columbia, MO 65211
EMC: 573-882-8304
Direct: 573-884-7338
Email: whiteto-at-missouri.edu
Web: http://emc.missouri.edu
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From: wsalmon-at-wi.mit.edu
Date: Tue, 21 Jan 2020 15:32:39 -0600
Subject: [Microscopy] 2020 Analytical and Quantitative Light Microscopy course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

There is still plenty of time to apply for Analytical and Quantitative
Light Microscopy 2020, held at the Marine Biological Laboratory, Woods
Hole, MA,
USA. The course will run April 28-May 8, 2020.

Applications are due February 5, 2020. Apply through the course web site at
http://www.mbl.edu/aqlm. We especially invite those from underrepresented
groups to apply. Reach out if you have any questions!

AQLM is a comprehensive and intensive course in light microscopy for
researchers in biology, medicine, and material sciences. This course
provides a systematic and in-depth examination of the theory of image
formation and application of digital methods for exploring subtle
interactions between light and the specimen. This course emphasizes the
quantitative issues that are critical to the proper interpretation of
images obtained with modern wide-field microscopes, confocal microscopes,
and new emerging technologies.

Applications are due February 5, 2020. Apply through the course web site at
http://www.mbl.edu/aqlm.

Some refer to it as "microscopy boot camp" because we work hard, but we
have a lot of fun in the process!

Course Directors:
Peter Kner, University of Georgia
Paul Maddox, University of North Carolina at Chapel Hill
Wendy Salmon, Whitehead Institute for Biomedical Research
Course Laboratory Director:
Gary Laevsky, Princeton University

Happy Imaging!
Peter, Paul, Wendy and Gary


Best,
Wendy

--------------------
Wendy C Salmon, M.A.
Light Microscopy Specialist
W.M. Keck Facility for Biological Imaging
Whitehead Institute for Biomedical Research
455 Main St., Rm 447
Cambridge, MA 02142
e: wsalmon-at-wi.mit.edu
w: microscopy.wi.mit.edu

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From: korinek-at-mcmaster.ca
Date: Tue, 21 Jan 2020 16:06:23 -0600
Subject: [Microscopy] CCEM TEM Summer School 2020

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The CCEM just announced that it will be holding its annual summer school on electron microscopy from June 8 - 12, 2020!

A 5-DAY COURSE for users with experience in electron microscopy, on the fundamentals of aberration-corrected imaging, electron energy loss spectroscopy, energy dispersive X-ray spectroscopy, electron tomography, ultimate physical limits (beam damage and resolution), DPC microscopy and the use of aberration-corrected electron microscopes. The aim is to provide students a device in solving characterization problems with the help of experts. The course will include lectures given by experts in the use of the technique and experts in electron optics, alignment and optimization of electron microscopes and EELS spectrometers. Students will have plenty of opportunities for hands-on training on the alignment and operation of the electron microscopes with the experts from the microscope and spectrometer companies. Two FEI Titan microscopes with correctors and monochromators (GIF Quantum and K2) will be used for training, as well as a Talos F200X. Several hands-on data processing sessions are also organised.

DATE: June 8 - 12, 2020

COST: All meals and course notes are included in the registration fee ranging from $700.CDN/full-time students to $2000.CDN/industry researchers. Accommodation will be separate and the responsibility of attendees (see full details on registration form).

REGISTRATION: Register online by January 31, 2020:
https://ccem.mcmaster.ca/ccem-summer-school-2020

--†
Dr. Andreas Korinek

Manager

Canadian Centre for Electron Microscopy
McMaster University
1280 Main Street West, Hamilton ON Canada, L8S 4M1
phone: +1 905-525-9140 ext 20400



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From: microscopy.listserver-at-gmail.com
Date: Wed, 22 Jan 2020 08:40:58 -0600
Subject: [Microscopy] viaWWW:Postdoc/Research Associate Position Available - McMaster

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Email: bassimn-at-mcmaster.ca

Name: Nabil Bassim

Organization: McMaster University

Title-Subject: [Filtered] Postdoc/Research Associate Position Available - McMaster University

Message: The Bassim research group is seeking a postdoc or research associate who will work on a
varied portfolio of projects, including developing novel 2-D van der Waals heterostructures,
catalysts, and metamaterials.
The chosen candidate would also be encouraged to develop their own microscopy techniques using the
CCEM infrastructure. The research projects involve strong collaboration with synthesis teams, and
proven ability to work in interdisciplinary groups should be demonstrated. Teaching, communication,
and presentation skills are part of a successful post-doctoral or research associate position, and
academic/industrial/governmental experience in these areas should be clearly detailed. Day-to-day
supervisory tasks with graduate students in the CCEM and within the Bassim research group would be
expected, and examples of administrative, project management, and supervisory skills would be a plus.
Requirements for the role include:
MANDATORY
- A Ph.D. in Materials Science or Physics
- Experience in aberration-corrected imaging and electron energy loss spectroscopy
- A strong track record in publishing TEM-related research

DESIRED
- Interest in mentoring graduate students
- Experience with in-situ techniques
- Capability to perform image simulations and scattering theory
- Sample preparation capabilities using FIB
Pay will range based on level of experience and track record. The appointment is for 1 year,
renewable up to 3 years.

Located at McMaster University in Hamilton, Ontario, the work would be performed at the Canadian
Centre for Electron Microscopy (CCEM), which is home to 10 technical staff and hundreds of users
with many diverse interests. The CCEM has a very elaborate suite of advanced instrumentation with
plans for future expansion. Hamilton is a lovely city in Southern Ontario, located midway between
Toronto and Niagara Falls with a mild (by Canadian standards) winter.

If you are interested, please contact bassimn at mcmaster.ca with a CV and several references.


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From: microscopy.listserver-at-gmail.com
Date: Fri, 24 Jan 2020 18:09:33 -0600
Subject: [Microscopy] viaWWW: FEI (Philips) XL30 Upgrade

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Email: wim5-at-lehigh.edu

Name: Bill Mushock

Organization: Lehigh Unbiversity

Title-Subject: [Filtered] FEI (Philips) XL30 Upgrade

Message: We are looking to extend the life of our WinNT XL30 ESEM with an upgrade. It seems FEI no
longer offers an upgrade but there are apparently several options available through third party vendors.

I would appreciate hearing directly from anyone willing to share their experiences who have gone the
third party route.

Also if you're contemplating decommissioning a Win 2000 XL30 in the near future and are looking for
someone to take it off your hands, please contact me.

Thanks,
Bill Mushock
wim5-at-lehigh.edu

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From: diller-at-stefan-diller.com
Date: Sat, 25 Jan 2020 06:21:46 -0600
Subject: [Microscopy] Re: viaWWW: FEI (Philips) XL30 Upgrade

Contents Retrieved from Microscopy Listserver Archives
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Hello Bill,

here in Germany there is a manufacturer who substitutes the old electronics of the XL30 (even ESEM) with a completely new one.

Refurbishing can also be done at your site and takes some days.

Please check at

https://www.pointelectronic.de/en/products/sem-control/

...Just a satisfied customer...


Best wishes,

Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 25.01.20 um 01:17 schrieb microscopy.listserver-at-gmail.com:
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} Email: wim5-at-lehigh.edu
}
} Name: Bill Mushock
}
} Organization: Lehigh Unbiversity
}
} Title-Subject: [Filtered] FEI (Philips) XL30 Upgrade
}
} Message: We are looking to extend the life of our WinNT XL30 ESEM with an upgrade. It seems FEI no
} longer offers an upgrade but there are apparently several options available through third party vendors.
}
} I would appreciate hearing directly from anyone willing to share their experiences who have gone the
} third party route.
}
} Also if you're contemplating decommissioning a Win 2000 XL30 in the near future and are looking for
} someone to take it off your hands, please contact me.
}
} Thanks,
} Bill Mushock
} wim5-at-lehigh.edu
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==============================Original Headers==============================
12, 28 -- From diller-at-stefan-diller.com Sat Jan 25 06:21:46 2020
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12, 28 -- Subject: Re: [Microscopy] viaWWW: FEI (Philips) XL30 Upgrade
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From: microscopy.listserver-at-gmail.com
Date: Sun, 26 Jan 2020 16:40:02 -0600
Subject: [Microscopy] viaWWW:UCT controller

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Email: xbren-at-uw.edu Name: Shirley

Organization: UW

Title-Subject: [Filtered] UCT controller
Message: Hi,
Can someone give me any ideas? One of my lab's ultra-cut microtome(Leica UCT) controller doesn't
work any more, and we found that the power unit is down. We reached Leica, but they said they don't
have this model and don't do repair on this model. The microtome is good, but it's only the
controller that doesn't work. Do you know where to repair/replace this kind of controller?

Many thanks!



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From: zaluzec-at-microscopy.com
Date: Tue, 28 Jan 2020 07:49:11 -0600
Subject: [Microscopy] viaWWW: EELS & EFTEM Analysis Training School April 2020

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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan, Inc.

Title-Subject: [Filtered] EELS & EFTEM Analysis Training School April 2020

Message: EELS & EFTEM Analysis Training School April 2020
April 21 ñ 24, 2020
Gatan R&D Headquarters, Pleasanton, CA

We invite you to our upcoming course to learn best practices to set up your EELS hardware, optimize
experimental protocols, then capture, and extract the maximum amount of compositional and chemical
information from your TEM samples. Topics include:

ï Fundamentals of EELS and energy-filtered imaging in TEM
ï Principles of operation of EFTEM and EELS systems
ï Optimization of EFTEM and EELS data acquisition
ï Quantification of elemental composition
ï Other information provided by EFTEM/EELS and how best to extract it
ï Use of EELS signals to form maps of elemental and chemical composition
ï EFTEM and STEM EELS spectrum imaging techniques
ï Identification of material phases via EELS fine structure mapping
ï Applications to biological and physical science specimens

Register today as seats are limited.

Registration: https://www.gatan.com/company/events/eels-eftem-analysis-training-school-april-2020


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From: microscopy.listserver-at-gmail.com
Date: Wed, 29 Jan 2020 09:13:05 -0600
Subject: [Microscopy] viaWWW: pathological report for mouse kidney EM

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Email: amaliapasolli-at-gmail.comm Name: Amalia Pasolli

Organization: The Rockefeller University

Title-Subject: [Filtered] pathological report for mouse kidney EM

Message: Hi,
Do you know any lab that could do TEM of mouse kidneys, including a pathology report?

Closer to NYC area would be a plus.

Thank you!

AMALIA
Hilda Amalia Pasolli, Ph.D.
Director and Research Associate Professor
Electron Microscopy Resource Center
RRB 120F
The Rockefeller University
1230 York Avenue,
Box 230
New York, NY 10065
Phone 212 327 8325

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From: microscopy.listserver-at-gmail.com
Date: Thu, 30 Jan 2020 09:08:59 -0600
Subject: [Microscopy] viaWWW:Job Vacancy - Optical Microscopist

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X-from: julie.blasioli-at-petermac.org

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Email: julie.blasioli-at-petermac.org Name: Julie Blasioli

Organization: Peter MacCallum Cancer Centre

Title-Subject: [Filtered] Job Vacancy - Optical Microscopist

Message: Location: Peter MacCallum Cancer Centre, Melbourne Australia

Peter MacCallum Cancer Centre is seeking an experienced and highly motivated scientist to join the
Centre for Advanced Histology and Microscopy (CAHM). CAHM encompasses research histology, optical
and electron microscopy and provides researchers with advice, tuition and technical expertise.

The applicant will have a passion for optical microscopy and in-depth understanding of the operation
of optical microscopes, including confocal, super resolution, and multiphoton microscopy. The
applicant will enjoy interacting with researchers and assisting them with their imaging needs.

The position is initially for a fixed term of 12 months with the opportunity for future extension.

Contact Person: Sarah Ellis
Contact Number: +613 8559 7822
Contact Email: sarah.ellis-at-petermac.org
Closing Date: 08 April 2020

To see the position description and apply go to
https://petermac.mercury.com.au/ViewPosition.aspx?id=heS2osrVG/U=&jbc=ere




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From: microscopy.listserver-at-gmail.com
Date: Thu, 30 Jan 2020 09:30:07 -0600
Subject: [Microscopy] Fwd: viaWWW: pathological report for mouse kidney EM

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X-from: Lee Cohen-Gould {lcgould-at-med.cornell.edu}



Hi Amalia-
Happy New Year.
What kind of pathology do you expect?† In the cortex or medulla?† Glomeruli, tubules or calex?
I can send you a protocol for mouse kidney, or we can do it here, but we don't do the pathology
analysis.† You should speak to the people in your animal facility. They should be able to either do
it or refer you to someone, perhaps at the Animal Medical Center.
I could put you in touch with our clinical EM facility here. They specialize in human kidney pathology.
Let me know what you need.
Best,
Lee

*Leona Cohen-Gould, MS, CEMT*

Senior Staff Associate

Co-Director CLC Microscopy & Image Analysis Core

*Weill Cornell Medicine*

Department of Biochemistry

1300 York Ave, A-105

New York, NY 10065

T 212.746.6146

F 212.746.8175

*lcgould-at-med.cornell.edu {mailto:lwschafe-at-med.cornell.edu} *
*
*
*

/If you have a publication or grant (submitted or funded) that utilized data from our Core, please
tell us here: /

{mailto:lwschafe-at-med.cornell.edu}

{mailto:lwschafe-at-med.cornell.edu} https://weillcornellmicroscopy.org/submit-publicationgrant-info


*



----------------------------------------------------------------------------------------------------
*From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
*Sent:* Wednesday, January 29, 2020 10:14 AM
*To:* Lee Cohen-Gould {lcgould-at-med.cornell.edu}
*Subject:* [EXTERNAL] [Microscopy] viaWWW: pathological report for mouse kidney EM



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Email: amaliapasolli-at-gmail.comm Name: Amalia Pasolli

Organization: The Rockefeller University

Title-Subject: [Filtered] pathological report for mouse kidney EM

Message: Hi,
Do you know any lab that could do TEM of mouse kidneys, including a pathology report?

Closer to NYC area would be a plus.

Thank you!

AMALIA
Hilda Amalia Pasolli, Ph.D.
Director and Research Associate Professor
Electron Microscopy Resource Center
RRB 120F
The Rockefeller University
1230 York Avenue,
Box 230
New York, NY 10065
Phone 212 327 8325

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From: microscopy.listserver-at-gmail.com
Date: Sat, 1 Feb 2020 20:32:23 -0600
Subject: [Microscopy] viaWWW: Help Needed - Error Code for SU1510

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Title-Subject: [Filtered] Error Code for SU1510

Message: We purchased a Hitachi SU1510 SEM about 4 years ago and have had some good luck with it but
recently our roughing pump has gone down and while now functionalÖ.we still canít get our scope to
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if anybody might have experienced such an error in the past and thus might know a fix that would
not require a Hitachi engineer and the associated cost.

Thanks in advance,

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From: microscopy.listserver-at-gmail.com
Date: Wed, 5 Feb 2020 08:45:18 -0600
Subject: [Microscopy] viaWWW:European Microscopy Congress 2020 - Abstract Submission

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Title-Subject: [Filtered] European Microscopy Congress 2020 - Abstract Submission Deadline 1 March 2020

Message: The European Microscopy Congress 2020 (emc2020) in Copenhagen is being hosted by SCANDEM,
and organised by the Royal Microscopical Society under the auspices of the EMS and IFSM. The
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showcasing the latest research in life sciences, physical sciences and engineering across all
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From: koeck-at-kth.se
Date: Thu, 6 Feb 2020 04:24:56 -0600
Subject: [Microscopy] electron source

Contents Retrieved from Microscopy Listserver Archives
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‚Äã

I'm looking for an electron source, used or new, that can produce a beam with the following characteristics:

energy: 1 keV or less
current: in the order of a few microAmps
beam waist diameter: 1 micrometer or less

I'm thinking of a gun and a single condenser lens if that is possible. Small is good.

Something used in a SEM, in e-beam lithography or electron evaporation might be a possibility.

Can anyone recommend something?

All the best,

Philip


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From: vray-at-partbeamsystech.com
Date: Thu, 6 Feb 2020 08:47:32 -0600
Subject: [Microscopy] Re: electron source

Contents Retrieved from Microscopy Listserver Archives
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Hi Keith,

Electron source with beam current of a few uA wouldn't exactly be from
SEM or EBL domain, but.... I would suggest to try speaking to: Kimball
Physics https://www.kimballphysics.com/

No connection other then a satisfied customer here.

Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 2/6/2020 5:25 AM, koeck-at-kth.se wrote:
} ----------------------------------------------------------------------------
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}
} I'm looking for an electron source, used or new, that can produce a beam with the following characteristics:
}
} energy: 1 keV or less
} current: in the order of a few microAmps
} beam waist diameter: 1 micrometer or less
}
} I'm thinking of a gun and a single condenser lens if that is possible. Small is good.
}
} Something used in a SEM, in e-beam lithography or electron evaporation might be a possibility.
}
} Can anyone recommend something?
}
} All the best,
}
} Philip
}
}
} ==============================Original Headers==============================
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From: vray-at-partbeamsystech.com
Date: Thu, 6 Feb 2020 08:47:31 -0600
Subject: [Microscopy] Re: electron source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Keith,

Electron source with beam current of a few uA wouldn't exactly be from
SEM or EBL domain, but.... I would suggest to try speaking to: Kimball
Physics https://www.kimballphysics.com/

No connection other then a satisfied customer here.

Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 2/6/2020 5:25 AM, koeck-at-kth.se wrote:
} ----------------------------------------------------------------------------
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} I'm looking for an electron source, used or new, that can produce a beam with the following characteristics:
}
} energy: 1 keV or less
} current: in the order of a few microAmps
} beam waist diameter: 1 micrometer or less
}
} I'm thinking of a gun and a single condenser lens if that is possible. Small is good.
}
} Something used in a SEM, in e-beam lithography or electron evaporation might be a possibility.
}
} Can anyone recommend something?
}
} All the best,
}
} Philip
}
}
} ==============================Original Headers==============================
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From: koeck-at-kth.se
Date: Fri, 7 Feb 2020 07:44:50 -0600
Subject: [Microscopy] SV: electron source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

An update to my question:

Does anybody have a microbeam instrument that's not needed anymore.
I'm interested in the column-part of it.

I need to produce an electron beam with the following characteristics:

energy: 1 keV or less
current: in the order of a few microAmps, up to 10 if possible.
beam waist diameter: 1 micrometer or less

The beam should be convergent with the waist at a distance of about 15 cm from the device.

All the best,

Philip

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From: vray-at-partbeamsystech.com
Date: Fri, 7 Feb 2020 13:30:54 -0600
Subject: [Microscopy] Re: Ask-a-Microscopist: FIB sample preparation

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Name: Aubrey Penn
Email: anpenn-at-ncsu.edu

Avoiding surface damage during FIB sample preparation is fairly
straight-forward: prior to ever exposing sample ion beam it should be
coated with sufficient thickness (~100nm optimal, but } 30nm is a minimal
requirement) of some protective layer that will "absorb" ion beam damage.

Following coatings may typically be used for such protective layer,
depending on nature of the sample: (a) e-beam deposition of C, Pt, Mo,
W, SiOx, etc...; (b) evaporated carbon or metal; (c) sputter coating by
Au, Au/Pd, TiO, Cr, Ir, etc...; (d) conductive polymer coating by
spinning or ultrasonic nozzle dispensing; (e) ink coating (i.e. "Sharpie
trick), etc...

For atomic resolution TEM amorphous layer on the sides of the lamella
would also need to be cleaned, but that is already another question.

Happy sample prepping :)
Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 2/7/2020 11:52 AM, oshel1pe-at-cmich.edu wrote:
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} Forwarded from "Ask a Microscopist"
} Please remember that the person asking the question is likely not a member the listserver, and
} **any reply should go directly to the poster** as well as to the list.
} Using the "reply" function in your email does *not* send your answer to the person asking the question.
} Please copy their email address from their question.
} ***********************************************************************************
} Name: Aubrey Penn
} Email: anpenn-at-ncsu.edu
} Subject: FIB sample preparation
} Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging?
}
} I'm not a FIB expert, but there are plenty on the microscopy list. There will probably be other people who would like to know the answer to this question, too, so please reply both directly to Penn and to the list.
}
} -------------
} Philip Oshel
} Microscopy Society of America
} Ask a Microscopist
} www(dot)microscopy(dot)org/resources/ask(dot)cfm
}
}
}
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From: vray-at-partbeamsystech.com
Date: Fri, 7 Feb 2020 13:30:54 -0600
Subject: [Microscopy] Re: Ask-a-Microscopist: FIB sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Avoiding surface damage during FIB sample preparation is fairly
straight-forward: prior to ever exposing sample ion beam it should be
coated with sufficient thickness (~100nm optimal, but } 30nm is a minimal
requirement) of some protective layer that will "absorb" ion beam damage.

Following coatings may typically be used for such protective layer,
depending on nature of the sample: (a) e-beam deposition of C, Pt, Mo,
W, SiOx, etc...; (b) evaporated carbon or metal; (c) sputter coating by
Au, Au/Pd, TiO, Cr, Ir, etc...; (d) conductive polymer coating by
spinning or ultrasonic nozzle dispensing; (e) ink coating (i.e. "Sharpie
trick), etc...

For atomic resolution TEM amorphous layer on the sides of the lamella
would also need to be cleaned, but that is already another question.

Happy sample prepping :)
Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 2/7/2020 11:52 AM, oshel1pe-at-cmich.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} ***********************************************************************************
} Forwarded from "Ask a Microscopist"
} Please remember that the person asking the question is likely not a member the listserver, and
} **any reply should go directly to the poster** as well as to the list.
} Using the "reply" function in your email does *not* send your answer to the person asking the question.
} Please copy their email address from their question.
} ***********************************************************************************
} Name: Aubrey Penn
} Email: anpenn-at-ncsu.edu
} Subject: FIB sample preparation
} Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging?
}
} I'm not a FIB expert, but there are plenty on the microscopy list. There will probably be other people who would like to know the answer to this question, too, so please reply both directly to Penn and to the list.
}
} -------------
} Philip Oshel
} Microscopy Society of America
} Ask a Microscopist
} www(dot)microscopy(dot)org/resources/ask(dot)cfm
}
}
}
} ==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Sat, 8 Feb 2020 09:15:44 -0600
Subject: [Microscopy] viaWWW: Invitation to Lehigh Microscopy School 2020

Contents Retrieved from Microscopy Listserver Archives
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Email: engineering-at-lehigh.edu

Name: Drs. Kiely and Watanabe, Lehigh University

Organization: Lehigh University

Title-Subject: [Filtered] Invitation to Lehigh Microscopy School 2020

Message: It is our great pleasure to invite you to the 50th annual Lehigh Microscopy School (LMS),
set for May 31-June 5, 2020, here in the Whitaker Laboratory at Lehigh University in Bethlehem, PA.

The school's 2020 offering marks a half century since renowned Lehigh University Professor Joe
Goldstein founded the annual weeklong program to keep colleagues and professionals abreast of
developments in scanning electron microscopy. Since then it has grown to become the largest school
of its kind.
Now in its 50th year, the weeklong school offers basic and advanced courses that combine theory with
lab practice. LMS is proud to partner with some of the top microscopy manufacturers in the world
supplement the nine scanning and transmission electron microscopes in Lehigh's Materials
Characterization Facility, one of the best equipped of its kind in the United States.

Please visit lehigh.edu/microscopy to learn more about LMS and its curriculum; please don't hesitate
to reach out if you have any questions.

We thank you for your interest and look forward to seeing you at LMS 2020!

Kind regards,

Dr. Christopher J. Kiely
Harold B. Chambers Senior Professor of Materials Science and Engineering
Lehigh University
LMS Director

Dr. Masashi Watanabe
Associate Professor of Materials Science and Engineering
Lehigh University
LMS Co-director



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From: microscopy.listserver-at-gmail.com
Date: Sat, 8 Feb 2020 09:16:36 -0600
Subject: [Microscopy] =?UTF-8?Q?viaWWW=3aSession_13d_=e2=80=9cAirborne_particles_and_fibe?=

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Email: elena.belluso-at-unito.it

Name: Elena Belluso

Organization: University of Torino - I

Title-Subject: [Filtered] session 13d ìAirborne particles and fibers: characteristics, sources,
toxicology, and impacts on ecosystems and human healthî, Goldschmidt 2020

Message: Dear Colleague,
We are pleased to invite you to submit an abstract for an oral presentation (or a poster) to the
session 13d: AIRBORNE PARTICLES AND FIBERS: CHARACTERISTICS, SOURCES, TOXICOLOGY, AND IMPACTS ON
ECOSYSTEMS AND HUMAN HEALTHî (Theme 13 https://goldschmidt.info/2020/program/programViewThemes) at
Goldschmidt 2020, the international conference on geochemistry and related subjects, scheduled from
21 to 26 June 2020 in Honolulu, Hawaii.

The key-note speaker of the session is Reto GierÈ (University of Pennsylvania): ìMineralogy,
Chemistry, and Toxicology of Particulate Emissions from Combustion Processesî.

Convenors: Elena Belluso (Universit‡ degli Studi di Torino, Torino, Italy), Janice Brahney (Utah
State University, Logan, UT, USA), Francesco Di Benedetto (Universit‡ degli Studi di Firenze,
Firenze, Italy), Peggy O'Day (University of California, Merced, CA, USA)

Note that the abstract deadline is February, 14, 2020 (https://goldschmidt.info/2020/abstracts)

We look forward to seeing you in Honolulu in June!


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From: microscopy.listserver-at-gmail.com
Date: Sat, 8 Feb 2020 09:17:25 -0600
Subject: [Microscopy] Fwd: Re: viaWWW:TEM Film Issue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Richard E. Edelmann {Edelmare-at-miamioh.edu}

Andrew,

There are really only three factors:

(1) The film. Is it old or bad? Has something changed in the development? (temp or developer aged
etc.)

(2) Is the Sens seeting on the scope still correct? Which you've chanegd all the way up to 20?
That is very high.

Finally I am betting it is:

(3) Is the beam current being correct read by the scope? As Page-1 of the CRT display shows the
Curr Dens as received on the screen. Does that adjust when you adjust the brightness control?

--} Secondly, there is an adjustment that is made to the Curr Dens reading when the focusing screen
is in the beam path vs when it is out. Does the vale look reasonable in vs out?
--} I have had both bad signal from the main viewing screen and the adjustment from the focusing
screen in vs out.




On 19 Dec 2019 at 8:52, microscopy.listserver-at-gmail.c wrote:

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} Email: AFELL-at-2SPI.COM Name: Andrew Fell
}
} Organization: Structure Probe
}
} Title-Subject: [Filtered] TEM Film Issue
}
} Message: I am working on a JEOL 1200-EX that still uses film for
} imaging. Recently, the film started to come out completely
} under-exposed. It was discovered that the film exposure setting had
} changed to 20 out of 20. Since then, we have tried many different
} parameters and have had no positive results as far as useful images.
} After adjusting the FSE we have an image again, but now we are
} struggling to obtain a scale of contrast. Despite being in-focus and
} displaying layers of contrast on screen - the film continues to
} develop in a more black-and-white situation.
}
} We have gone through changing accelerating voltage, new film, new
} developing chem, exposure times, the full range of FSE, aperture
} adjustments and we are still hitting a wall. Has anyone dealt with
} this or (preferably) know how to resolve this issue? Thank you in
} advance!
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Richard E. Edelmann, Ph.D., Director
Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-miamioh.edu
http://www.cami.muohio.edu


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From: crwinkler-at-ncsu.edu
Date: Mon, 10 Feb 2020 15:54:50 -0600
Subject: [Microscopy] Re: Ask-a-Microscopist: FIB sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Aubrey,

Your Question: What are the best ways to avoid damaging or outright
destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for
atomic resolution STEM imaging?

I'm going to answer this in several parts.

You don't say what the material or the substrate is. I assume that it is a
semiconductor. If it is and the sample doesn't have to be site-specific,
then the best way to prepare a 20 nm thick epitaxial film and avoid Ga
damage is not to use the FIB at all.

1. The absolute best way to prepare your sample with no amorphous damage at
all is the Small Angle Cleavage Technique originally developed by John
McCaffrey and then modified quite a bit by John and myself. A major
disadvantage of this technique is that there is no amorphous damage and so
it can be difficult to focus and stigmate in a field emission TEM. I will
sometimes put a thin layer of ca carbon on the top surface just to have
something amorphous that I can use in the FFT to help focus and stigmata.
If you want, I can send you a detailed presentation on how to do the
technique. Just send a request to my work email:
(scott-dot-d-dot-walck2-dot-ctr-at-mail-dot-mil).

2. The next best way of preparing your sample would be low angle, low energy
Ar ion milling developed by Arpad Barna. Now, all the manufacturers of ion
mills have low angle, low energy capabilities. Arpad has several
publications out that show the amorphous damage at different energies and
angles.

3. OK, so you have a FIB and you don't want to learn how to make samples the
old fashioned way. You have to protect the top layer from ion damage before
putting on the ion beam Pt (or W) protective layer. You can put it on by
e-beam deposition. All you need is 200-250 nm thick to stop and ions from
hitting the top surface. Just don't let the ion beam hit the surface with
too high of a current or for very long (minimize the dosage to the surface
or you will erode the protective layer.) If your sample is not site specific
and you don't want to have a high Z material next to your very thin layer,
you should consider putting carbon down. Any easy way to do this is to use
a Sharpie(R) pen and coat the surface before you put it in the FIB. This
works, but can lead to open areas and what looks a bit stringy. A better
way is to use a carbon coater to put a uniform layer down. We have a Leica
ACE 600 and I just measured the thickness of carbon using a double carbon
thread and using it all up. It gave a thickness of 390 nm, which is plenty.
At any rate, putting the carbon layer next to your thin layer gives a better
contrast between the layer of interest and the protective top layer to see
it better. The high Z of Pt or W can make it difficult to see a very thin
layer at the top of the surface of your sample.

When preparing the sample by FIB, you will have an amorphous damage layer on
top and bottom with a thickness that is dependent on the energy of the beam
that you last use. The newest FIBs are capable of "polishing" with a 2 keV
(or lower) Ga beam to minimize the damage layer. It is done by exposing the
two surfaces at an angle of 3∞. Samples prepared this way are pretty good,
but remember, there is still an amorphous layer on both the top and bottom
surfaces.

There are also other ways of removing FIB damage from the surfaces of FIB
lamella using Ar ion milling. In decreasing order of cost:

-E.A. Fischione makes the NanoMill and the PicoMill, which use a
scanning Ar beam to polish the surfaces with low energy Ar. They have
presentations and product information on these tools.

-Several manufacturers of ion mills, specifically Gatan and
Technoorg Linda of recipes for removing damage using their tools. You
should contact them for papers and presentations on how to do that.

-Another technique that I patented while working at South Bay
Technology is the Plasma Trimming method, which uses the shape of the FIB
sample and a bias to create a field that causes ions from a plasma to polish
the two surface of the sample. The plasma was generating using Ar gas in a
plasma cleaner. This patent is now owned by Ted Pella, Inc. I can provide
a Plasma Trimming presentation upon request.
(scott-dot-d-dot-walck2-dot-ctr-at-mail-dot-mil)

BTW, you can estimate the amount of damage and depth for different ions,
energies, and angles by using SRIM and TRIM calculations. See www.srim.org
for more information and to download the software and manual.

I hope that this helps. I'm sure it is more than you need.

-Scott






-----Original Message-----
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Sent: Friday, February 7, 2020 11:55 AM
To: s.walck-at-comcast.net

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Forwarded from "Ask a Microscopist"
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Name: Aubrey Penn
Email: anpenn-at-ncsu.edu

Hello Aubrey,

Good to see you on the listserv!

In regards to your question, Valery and Scott gave you some good
advice. I've always referred students to the following paper:
https://www.sciencedirect.com/science/article/abs/pii/S030439911200006X
I can give you a copy the next time I see you.

There are a lot of ways to skin a cat, as they say, but it can be
challenging to get good FIB samples if you're used to the results
generated by tripod polishing and low energy, angle ion milling. The
best results I've seen are from FIB samples prepared in a similar
manner as in the paper above, followed by a cleanup in a low energy
ion mill (Fischione, Gatan, Technoorg-Linda...all are fine). However,
the ion mill cleanup is not magic. You can start to etch your liftout
if the angle and voltages are not ideal, or if you mill for too long
to compensate for a thicker liftout.

Depending on the materials, you may also be able to dip the liftout in
a dilute acid or base to thin the liftout, or use a flash
electropolishing setup like the one they have at PNNL to reduce the
liftout thickness and remove damage layers. Regardless of what
post-FIB step you use, you need to do your best to create a thin, flat
liftout with a minimal amount of sputter redep in the first place, and
that's where that paper gives some valuable tips.

Good luck,
Chris


On Fri, Feb 7, 2020 at 10:52 AM {oshel1pe-at-cmich.edu} wrote:
}
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} Name: Aubrey Penn
} Email: anpenn-at-ncsu.edu
} Subject: FIB sample preparation
} Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging?
}
} I'm not a FIB expert, but there are plenty on the microscopy list. There will probably be other people who would like to know the answer to this question, too, so please reply both directly to Penn and to the list.
}
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} Microscopy Society of America
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--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
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Cell: 267-496-0587


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From: microscopy.listserver-at-gmail.com
Date: Sun, 22 Mar 2020 12:49:07 -0500
Subject: [Microscopy] viaWWW:Covid-19 virus

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Email: xbren-at-uw.edu Name: Shirley Ren

Organization: University of Washington

Title-Subject: [Filtered] Covid-19 virus
Message: Dear Colleagues,
Has someone found the virus under TEM? Is it possible to do RNA-FISH on thick sections (Epoxy
embedded block)?
Many thanks,

Shirley


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From: microscopy.listserver-at-gmail.com
Date: Tue, 24 Mar 2020 08:31:14 -0500
Subject: [Microscopy] Fwd: Re: Fwd: Re: viaWWW: Teaching SEM Online

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X-from: Beck, Stephen J. {Stephen.Beck-at-ncc.edu}

Hi David,

Thanks for the reply. I’m not sure how I could get samples to you. My students were just working on
getting samples prepared. My microbial/Paramecium prep was canceled by the corona virus. My students
and I can’t even get on campus at present.
Do you have any “stock” biological samples on hand? Can you even get to your own scopes? My students
have required samples to image but I’m sure I’ll have to cut that back given the current situation.
They would image 2 soft tissues, a cryofractured soft tissue, hard tissue, botanical sample (leaf
stomates), pollen, microbial/Paramecium, insect, diatoms.
Perhaps I could do a trial demo with you initially?

Thanks,

Steve

Stephen J. Beck
Professor
Coordinator, Bio-Imaging Center
Electron Microscopy Department of Biology
Nassau Community College
Garden City, NY 11530

Sent from my iPhone - thanks Steve (1955-2011)!

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} Hi Stephen,
}
} We have a couple of our SEMs connected to the internet for remote access. Through this interface,
} the microscope is completely controllable from your own PC. We can load your samples and then you or
} your students would be able to take control of the instrument and practice from your own homes.
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} Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck
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} Organization: Nassau Community College
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} Title-Subject: [Filtered] Teaching SEM Online
}
} Message: Dear Colleagues,
} I am teaching my SEM course this semester and like many institutions, we are trying to teach
} online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam
} online,
} however, after that we need to get on the SEM to image the required samples.
} Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
} this is impossible - I haven't received the courtesy of a response yet!
}
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From: microscopy.listserver-at-gmail.com
Date: Tue, 24 Mar 2020 08:35:23 -0500
Subject: [Microscopy] viaWWW:I'd like to help with Corona virus research

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Email: drk-at-shcc.org Name: Doug Keene

Organization: Shriners Hospital for Childern

Title-Subject: [Filtered] I'd like to help with Corona virus research

Message: Hello Everyone,

I'd like to apply my talents in Microscopy (TEM, SEM, Confocal, Micro-CT) to the Corona pandemic.
How do I get the word out that I would like to contribute?

Thanks,

Doug Keene
Shriner Hospital for Children
Portland, Oregon
503-819-3600 (cell)

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From: microscopy.listserver-at-gmail.com
Date: Tue, 24 Mar 2020 08:26:44 -0500
Subject: [Microscopy] Fwd: Re: Fwd: Re: viaWWW: Teaching SEM Online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

It seems that you replied to the entire list.
Since you did, there should be many scopes in many labs that should be able to help. The FEI scopes
are setup to use UltraVNC as part of their RAPID support service. It can be used just as well by lab
staff and clients for their own purposes. (In fact, I have used it more for my own benefit than FEI
has ever used it for theirs.) It would need to be setup properly with a single computer controlling
the computer. Multiple others might be able to watch along.
I would offer to help, but I am in the materials science area and would barely know what I am
looking at. We also do not have samples handy for demo purposes. I have a counterpart that would
have such expertise and samples, but she runs a Hitachi SEM and it is not setup for remote control
or viewing.
Warren Straszheim, Ph.D., manager
Materials Analysis and Research Lab
Iowa State University
515-294-8187


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, March 24,
2020 8:32 AM
To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

X-from: Beck, Stephen J. {Stephen.Beck-at-ncc.edu}

Hi David,

Thanks for the reply. I’m not sure how I could get samples to you. My students were just working on
getting samples prepared. My microbial/Paramecium prep was canceled by the corona virus. My students
and I can’t even get on campus at present.
Do you have any “stock” biological samples on hand? Can you even get to your own scopes? My students
have required samples to image but I’m sure I’ll have to cut that back given the current situation.
They would image 2 soft tissues, a cryofractured soft tissue, hard tissue, botanical sample (leaf
stomates), pollen, microbial/Paramecium, insect, diatoms.
Perhaps I could do a trial demo with you initially?

Thanks,

Steve

Stephen J. Beck
Professor
Coordinator, Bio-Imaging Center
Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530

Sent from my iPhone - thanks Steve (1955-2011)!

} On Mar 22, 2020, at 12:46 PM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
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} X-from: David Huskisson {david-at-emanalytical.co.uk}
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} Hi Stephen,
}
} We have a couple of our SEMs connected to the internet for remote
} access. Through this interface, the microscope is completely
} controllable from your own PC. We can load your samples and then you or your students would be able to take control of the instrument and practice from your own homes.
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} Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name:
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} Organization: Nassau Community College
}
} Title-Subject: [Filtered] Teaching SEM Online
}
} Message: Dear Colleagues,
} I am teaching my SEM course this semester and like many institutions, we are trying to teach
} online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam
} online,
} however, after that we need to get on the SEM to image the required samples.
} Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
} this is impossible - I haven't received the courtesy of a response yet!
}
} Thanks!
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From: microscopy.listserver-at-gmail.com
Date: Tue, 24 Mar 2020 08:33:05 -0500
Subject: [Microscopy] Fwd: E coli capsule polysaccharide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: hiro uryu {hiro.uryu-at-emscopic.com}


Dear List,

I have tried to visualize E cloi capsular polysaccharide by following the protocol from the book
chapter "Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell" by Sven
Hammerschmidt and Manfred Rohde from the published book entitled "Streptococcus pneumoniae, Method
and protocol" published by Humana Press. I compared alcian blue and ruthenium red in the presence or
absence of lysine.

At least in this first trial, ruthenium red in the presence of lysine appeared to be superb among
all conditions tested in our study setup. However, based on the EM analysis, I sensed that the
preservation of CPS is suboptimal and suggesting room for improvement.

I was wondering if CPS experts could give me any suggestions about how I can improve the
presentation of CPS. Many thanks,

Sincerely,
Hiro
------
Kunihiro Uryu,


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From: microscopy.listserver-at-gmail.com
Date: Tue, 24 Mar 2020 09:09:21 -0500
Subject: [Microscopy] Fwd: Re: Fwd: E coli capsule polysaccharide

Contents Retrieved from Microscopy Listserver Archives
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X-from: Rob Mesman {R.Mesman-at-science.ru.nl}

Dear Hiro,
I would recommend checking the review Ruthenium Red and the Bacterial Glycocaly by Fassel et al.
(1999) https://doi.org/10.3109/10520299909047974
it compares several different fixation and staining protocols with indications of what works best
for which type of CPS.
best regards!
Rob

---
Dr. Rob Mesman
Post-Doc
Department of Microbiology
Faculty of Science
Radboud University Nijmegen
Heyendaalseweg 135
NL-6525 AJ Nijmegen
The Netherlands

On 2020-03-24 14:43, microscopy.listserver-at-gmail.com wrote:
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} X-from: hiro uryu {hiro.uryu-at-emscopic.com}
}
}
} Dear List,
}
} I have tried to visualize E cloi capsular polysaccharide by following
} the protocol from the book
} chapter "Electron Microscopy to Study the Fine Structure of the
} Pneumococcal Cell" by Sven
} Hammerschmidt and Manfred Rohde from the published book entitled
} "Streptococcus pneumoniae, Method
} and protocol" published by Humana Press. I compared alcian blue and
} ruthenium red in the presence or
} absence of lysine.
}
} At least in this first trial, ruthenium red in the presence of lysine
} appeared to be superb among
} all conditions tested in our study setup. However, based on the EM
} analysis, I sensed that the
} preservation of CPS is suboptimal and suggesting room for improvement.
}
} I was wondering if CPS experts could give me any suggestions about how
} I can improve the
} presentation of CPS. Many thanks,
}
} Sincerely,
} Hiro
} ------
} Kunihiro Uryu,
}
}
} ==============================Original
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From: gilpin-at-purdue.edu
Date: Tue, 24 Mar 2020 09:14:41 -0500
Subject: [Microscopy] remote control of FEI TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,
I have a full FEI remote package that we bought with our Themis Z but I wondered if there was another solution to remote TEM that people are using. I have a spare set of hand panels already.

Chris
He/Him/His
Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility



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From: wij.muss-at-aon.at
Date: Tue, 24 Mar 2020 09:57:52 -0500
Subject: [Microscopy] Re: E coli capsule polysaccharide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dr. Kunihiro Uryu,
seconding Dr. Rob Mesman: fixation conditions…

Unfortunately not in possession of the protocol Dr. Kunihiro Uryu quoted*) only „active working“ colleagues might have the protocol in their collection, read and apply or have an answer for your request.

*) only for convenience:
https://www.ncbi.nlm.nih.gov/pubmed/30929202
Methods Mol Biol. 2019;1968:13-33. doi: 10.1007/978-1-4939-9199-0_2.

Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell.
Hammerschmidt S1, Rohde M2.
Author information
Abstract
Electron microscopy allows for studying bacterial ultrastructure at high resolutions. Two types of electron microscopes are used for this purpose. The transmission electron microscope allows for access to inner bacterial ultrastructure when imaging ultrathin sections as well as cell wall-attached structures by negative staining, whereas scanning electron microscopy allows for the detection of structures on the bacterial cell surface alone or to study the interplay between pneumococci and their host cells. This chapter deals with recommendations for well-adapted methodologies to examine pneumococcal ultrastructure in detail. Especially, we focus on the preservation of the pneumococcal capsular polysaccharide, which represents an important virulence factor of pneumococci. Since capsules are highly hydrated structures, the introduction of a new fixation protocol involving lysine acetate, ruthenium red, and osmium (LRR fixation) results in a very well-preserved capsular structure in such a way that the amount of capsular material bound on the bacterial surface can be compared within different serotypes. In our method, capsular ultrastructure is preserved without the need for serotype-specific antibodies, which have been used in other studies to preserve the pneumococcal capsule. In addition, the new LRR fixation allows for studying the presence or absence of capsular material during adhesion and invasion of pneumococci on epithelial or endothelial host cells in cell culture experiments.
KEYWORDS: Critical point drying; Cryo-FESEM; Field emission scanning electron microscopy; Infection; LRR embedding; LRWhite resin; Pneumococcal capsule; Pneumococci; Transmission electron microscopy
PMID: 30929202
DOI: 10.1007/978-1-4939-9199-0_2
[Indexed for MEDLINE]
⇒SPRINGER:
https://link.springer.com/protocol/10.1007%2F978-1-4939-9199-0_2

It would be interesting whether you tried "additives" like e.g. TA, La+++ and / or PPD (at least for a part of your specimens) with regard to fixation of the bacteria**)
(ok, I know that E. coli is something different from....but you might have a look into my Poster
https://www.researchgate.net/publication/215824406_Purpura_fulminans_ultrastructural_findings_by_transmission_electron_microscopy_in_four_cases_with_special_reference_to_fixation_of_skin_biopsies
and the oral presentation, uploaded into RG
https://www.researchgate.net/publication/281120633_Oral_Lecture_PURPURA_FULMINANS_Ultrastructural_findings_by_Transmission_Electron_Microscopy_in_4_cases_with_special_reference_to_fixation_of_skin_biopsies

Eventually you might have tried also the variation (".....substituting ruthenium red with a 0.5% alcian blue pyridine variant (pH 7.2).), cf.
https://www.researchgate.net/publication/313875937_Molecular_characterization_of_invasive_capsule_null_Neisseria_meningitidis_in_South_Africa
https://www.researchgate.net/figure/Transmission-electron-micrographs-showing-the-presence-of-surface-capsular-polysaccharide

Beware of Covid-19/SARS-CoV-2 infection,
keep distance and
STAY WELL,

best wishes AND best regards

Wolfgang Muß (MUSS)
Retired Member of MSA
SALZBURG, Austria


**) what I wanted to say with this is only pointing to other possibilities for retaining otherwise eluted "cellular or matricial substrate(s) in preparation for TEM....
(Sorry if I bothered anyone in this time of obviously global crisis)





======================================================================
Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com]
Gesendet: Dienstag, 24. März 2020 15:10
An: wij.muss-at-aon.at
Betreff: [Microscopy] Re: E coli capsule polysaccharide

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X-from: Rob Mesman {R.Mesman-at-science.ru.nl}

Dear Hiro,
I would recommend checking the review Ruthenium Red and the Bacterial Glycocaly [Glycocalyx] by Fassel et al.
(1999) https://doi.org/10.3109/10520299909047974
it compares several different fixation and staining protocols with indications of what works best for which type of CPS.
best regards!
Rob

---
Dr. Rob Mesman
Post-Doc
Department of Microbiology
Faculty of Science
Radboud University Nijmegen
Heyendaalseweg 135
NL-6525 AJ Nijmegen
The Netherlands

On 2020-03-24 14:43, microscopy.listserver-at-gmail.com wrote:
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} X-from: hiro uryu {hiro.uryu-at-emscopic.com}
}
}
} Dear List,
}
} I have tried to visualize E cloi capsular polysaccharide by following
} the protocol from the book chapter "Electron Microscopy to Study the
} Fine Structure of the Pneumococcal Cell" by Sven Hammerschmidt and
} Manfred Rohde from the published book entitled "Streptococcus
} pneumoniae, Method and protocol" published by Humana Press. I
} compared alcian blue and ruthenium red in the presence or absence
} of lysine.
}
} At least in this first trial, ruthenium red in the presence of lysine
} appeared to be superb among all conditions tested in our
} study setup. However, based on the EM analysis, I sensed that the
} preservation of CPS is suboptimal and suggesting room for improvement.
}
} I was wondering if CPS experts could give me any suggestions about how
} I can improve the presentation of CPS. Many thanks,
}
} Sincerely,
} Hiro
} ------
} ,
}
}
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From: matthew.weyland-at-monash.edu
Date: Tue, 24 Mar 2020 20:43:06 -0500
Subject: [Microscopy] Re: remote control of FEI TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Chris,

While there are many software solutions for sharing desktops, they all
fail a similar hurdle - you have to have your microscope PC connected
to the network directly. This is NOT a good idea as your Themis computer
will not be protected from the internet.

Over the last few months I've been suing a different solution on our
"Classic" Titan cubed. Making use of KVM over IP gear. These consist of
sender boxes that have HDMI and USB (and audio) inputs that connect (and
can be powered by) Cat5 cables to your network. Then any number of
receiver boxes can be used with HDMI/USB outputs anywhere you have a
network port. These systems have MANY advantages over the FEI remote
software;

1) They are FAST - they do real time up to 4k - not just 10fps you will
be lucky to get over VNC
2) There is no intrinsic connection between the microscope and the
network - as such there is no security issue (except if someone managed
to work out your IP/passwords for the boxes, or put a virus on a USB
stick - but this is just be careful..)
3) You can have multiple receivers for every sender - so many people can
watch each instrument.
4) USB can be used for the hand panels, keyboard and mice for the computers.
5) Partner these with HDMI switches behind monitors and you can switch
between local/remote super quickly.
6) They are microscope/instrument agnostic - if the instrument uses USB
and HDMI etc it will work.

Downsides;

1) They work with little configuration inside a subnet (such an
individual building - our situation where we have been running the
instrument from multiple different locations due to building work) but
if you are outside the subnet you'll need some help from your network
people.
2) The boxes cost - on the order of $500 for each box. But compared to a
microscope..
3) And you'll need one sender for each screen.

I've been using boxes made by Kramer (I have NO connection to this
company) but there are many manufacturers (Lindy, geffen etc). They are
mostly used is point of sale displays (all those screens at malls and
movie theatres) so they are pretty ubiquitous. I have three boxes (left
screen, right screen and accessory screen which runs Gatan, Bruker,
EMPAD AND support computer through a remote controlled switch). This has
worked really well. Let me know if you have any follow up questions.

Cheers

Matthew

--
Dr Matthew Weyland
Joint Deputy Director - Monash Centre for Electron Microscopy
Associate Professor - Department of Materials Science and Engineering

Monash Centre for Electron Microscopy
10 Innovation Walk, Clayton Campus
Monash University
Clayton VIC 3800 Australia

T: +61 3 9905 9026
E: matthew.weyland-at-monash.edu
mcem.monash.edu



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hi everyone,
I have a full FEI remote package that we bought with our Themis Z but I
wondered if there was another solution to remote TEM that people are
using. I have a spare set of hand panels already.

Chris
He/Him/His
Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility



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From: bradford.ross-at-botany.ubc.ca
Date: Tue, 11 Feb 2020 14:26:15 -0600
Subject: [Microscopy] Core Facility Research Tech Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers,

After 12+ great years at the UBC BioImaging Facility, I am moving on to a new position in the
private sector next month! As such, my position here is available now and has been posted to the UBC
HR website:

https://www.hr.ubc.ca/careers-postings/staff-s.php Posting ID: 36809

This is a great opportunity for someone with experience in various advanced TEM techniques including
electron tomography, cryo TEM, HRTEM, and their associated sample prep and image processing
techniques. We have also just moved the facility into brand new lab space, so the world will be your
oyster! There is opportunity to work with our SEM and optical microscope instruments as well.

Concurrently, we are also looking for an optical microscopy specialist to run our three laser
scanning confocal instruments, including an Olympus Multiphoton system, a Perkin-Elmer spinning
disk, and their associated equipment. https://www.hr.ubc.ca/careers-postings/staff-s.php Posting ID:
36794

I am happy to answer other questions about the TEM tech position, but please direct any formal
inquiries to our facility manager, Miki Fujita. BIF.manager-at-ubc.ca
Cheers,
Bradford Ross
Electron Microscopy Technician
BioImaging Facility
University of British Columbia
Biological Sciences Rm. 0120
6270 University Blvd. Vancouver, B.C. V6T 1Z4
phone 604-822-6996
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From: koeck-at-kth.se
Date: Tue, 18 Feb 2020 09:24:20 -0600
Subject: [Microscopy] SAD-aperture assembly

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

​Hi.


Does anybody happen to have an SAD-aperture assembly (aka field limiting aperture assembly,
manual version) for a Jeol JEM2100F lying outside the microscope at the moment?


I would be really interested in a photo of it with some measurements,
mainly the diameter and length of the rod.


Alternatively, a sketch with dimensions would also be great.


All the best,


Philip


PS: I'm trying to avoid opening up the microscope and getting drawings from Jeol is a lengthy procedure.


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From: xinran.liu-at-yale.edu
Date: Wed, 25 Mar 2020 08:44:08 -0500
Subject: [Microscopy] EM research associate position at Yale University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear Collogues,

The Coln-Ramos lab at Yale University is looking for a research associate to provide ongoing
electron microscopy work for a variety of projects in the lab. Under the direction of the Principal
Investigator and working in collaboration with laboratory personnel, this position will be in charge
of the design, collection, optimization and interpretation of electron microscopy research in the
Coln-Ramos lab as it pertains to the ultrastructure of C. elegans nervous system. The position will
prepare and process C. elegans worms for electron microscopy to better understand the function and
structure of the C. elegans nervous system. More information about the position can be found here:
https://www.nature.com/naturecareers/job/electron-microscopy-research-associate-yale-university-715443

Thanks for your attention.


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From: microscopy.listserver-at-gmail.com
Date: Wed, 19 Feb 2020 05:18:43 -0600
Subject: [Microscopy] viaWWW: Postdoctoral position at the Ames Laboratory

Contents Retrieved from Microscopy Listserver Archives
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X-from: tprozoro-at-ameslab.gov

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Email: tprozoro-at-ameslab.gov Name: Tanya Prozorov

Organization: Ames Laboratory

Title-Subject: [Filtered] Postdoctoral position at the Ames Laboratory

Message: The Division of Materials Science and Engineering (DMSE) at the Ames Laboratory, a
Department of Energy National Laboratory affiliated with Iowa State University, is searching for a
qualified Postdoctoral Research Associate.

We are looking for a motivated postdoctoral research associate to work on two projects focused on
characterization of structure and liquid phase dynamics of beam-sensitive materials by using
electron microscopy in-situ. The first project involves spatio-chemical characterization of
polymer-nanoparticle complexes and DNA origami nanostructures in liquid phase. The second project
involves monitoring cation mobility in low-contrast layered minerals in liquid phase and in-situ. We
are looking for applicants with hands-on experience using aberration-corrected scanning transmission
electron microscopy (S/TEM), electron energy loss spectroscopy (EELS), and energy dispersive
spectroscopy (EDS).
More details and application instructions can be found here:
https://isu.wd1.myworkdayjobs.com/en-US/IowaStateJobs/job/Ames-IA/Postdoctoral-Research-Associate---Ames-Laboratory_R1870

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From: xinran.liu-at-yale.edu
Date: Wed, 25 Mar 2020 08:48:34 -0500
Subject: [Microscopy] Re: EM research associate position at Yale University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
I apologize for a job posting that appears containing an invalid link. Thanks for those of you who
brought it to my attention. Here is the link to the Yale HR website, and it works.
https://sjobs.brassring.com/TGnewUI/Search/Home/Home?partnerid=25053&siteid=5248#jobDetails=1405083_5248.

Thanks you for your attention.

Xinran Nick Liu, M.D. & Ph.D.
Director, Center for Cellular and Molecular Imaging
Bio & Cryo Electron Microscopy Core Facilities
Yale University School of Medicine
Office: (203) 785-4050
Lab: (203) 785-5390
http://medicine.yale.edu/ccmi/em




On 2/18/20, 1:46 PM, "Liu, Xinran" {xinran.liu-at-yale.edu} wrote:

Dear Collogues,
The Colón-Ramos lab at Yale University is looking for a research associate to provide
ongoing electron microscopy work for a variety of projects in the lab. Under the direction of the
Principal Investigator and working in collaboration with laboratory personnel, this position will be
in charge of the design, collection, optimization and interpretation of electron microscopy research
in the Colón-Ramos lab as it pertains to the ultrastructure of C. elegans nervous system. The
position will prepare and process C. elegans worms for electron microscopy to better understand the
function and structure of the C. elegans nervous system. More information about the position can be
found here:
https://www.nature.com/naturecareers/job/electron-microscopy-research-associate-yale-university-715443
Thanks for your attention.


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11, 77 -- From: "Liu, Xinran" {xinran.liu-at-yale.edu}
11, 77 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
11, 77 -- Subject: Re: EM research associate position at Yale University
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From: microscopy.listserver-at-gmail.com
Date: Wed, 19 Feb 2020 14:44:58 -0600
Subject: [Microscopy] viaWWW:Stitching confocal volumes of organ in 3 dimensions

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Email: dkc25-at-cam.ac.uk Name: Darran Clements

Organization: University of Cambridge

Title-Subject: [Filtered] Stitching confocal volumes of organ in 3 dimensions

Message: Hi All,
just wanted to throw out a general question to the list. We are currently considering embarking on a
project where we have been asked to reconstruct an organ from mosaics of confocal volumes in serial
sections. We can stitch volumes in each section together easily enough, however, I wanted to ask if
anyone else has experience of then stitching these kinds of volumes together to reconstruct the
whole organ volume, and if so, could they impart some advice on what they found works best.

We are looking at slices of tissue around 100 microns thick and around 80-100 of these volumes
stitched together, then stitching multiple of these volume mosaics, one above the other, to
reconstruct the tissue.

We're mainly looking at the software aspect of this but of course, tangential replies and
alternative ideas are more than welcome too.

Many thanks
Darran

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From: bozhilov-at-ucr.edu
Date: Wed, 25 Mar 2020 08:52:53 -0500
Subject: [Microscopy] Re: question about EDS in SEM

Contents Retrieved from Microscopy Listserver Archives
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By the way, the solid angle would be the active area of the detector divided by the square of the
distance to specimen.

Krassimir.


} On Feb 19, 2020, at 2:45 PM, nizets2-at-yahoo.com wrote:
}
}
}
}
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} Dear colleagues,
}
} I'd have a simple question regarding the detector area of EDX (EDS) systems in SEM:
} Clearly the largest the detector, the highest the sensitivity for everything else equal but what if everything else was NOT equal?
}
} One clear example:
}
} - detector 1: 30mm², optimal distance to pole piece 10mm
} - detector 2: 150mm², optimal distance to pole piece 15mm
}
} Here the largest detector is also the furthest!
}
} I don't know how to appreciate the influence of the 2 factors, detector area and work distance, on the final sensitivity of the system.
} In other words, is the extra $$$ for the larger detector worth it if the work distance is at the same time increased?
}
} Many thanks in advance.
} Stephane
}
}
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From: microscopy.listserver-at-gmail.com
Date: Wed, 25 Mar 2020 08:54:35 -0500
Subject: [Microscopy] Fwd: Traceable Standard Specimen for TEM calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Erico Freitas {ericotadeu-at-ufmg.br}


Dear all,


We are looking after a traceable standard specimen for TEM calibration. We had a MAG*I*CAL sample
that got damaged, and it is in a temporary backorder at the EMSDiasum website.

Does any of you know and have used another traceable standard specimen for TEM calibration?


Thank you,

--
Erico Freitas

Physicist/Microscopist at Center of Microscopy
Universidade Federal de Minas Gerais (UFMG)
Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901.
+55-31-3409-7573
+55-31-3409-7575

Coordinator:Transmission Electron Microscopy laboratory

CV Lattes: *http://lattes.cnpq.br/8786127123101199*
{https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#}
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From: zaluzec-at-microscopy.com
Date: Wed, 25 Mar 2020 09:00:46 -0500
Subject: [Microscopy] Re: question about EDS in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Stephane

Go to this WWW site. It is an on-line solid angle calculator for XEDS systems.

http://www.zaluzec.com/NJZTools/

It will give you an unbiased calculation of solid angles.

Importantly I must contradict Krassimir, Area/distance^2 is incorrect, that is an approximation
which quickly breaks down for large detectors and small distances.

On that Solid Angle WWW site is also a link to a paper you might want to read.

Cheers

Nestor
Your Friendly Neighborhood SysOp

On 2/20/20 9:40 AM, nizets2-at-yahoo.com wrote:
} ----------------------------------------------------------------------------
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} Dear colleagues,
}
} I'd have a simple question regarding the detector area of EDX (EDS) systems in SEM:
} Clearly the largest the detector, the highest the sensitivity for everything else equal but what if everything else was NOT equal?
}
} One clear example:
}
} - detector 1: 30mm², optimal distance to pole piece 10mm
} - detector 2: 150mm², optimal distance to pole piece 15mm
}
} Here the largest detector is also the furthest!
}
} I don't know how to appreciate the influence of the 2 factors, detector area and work distance, on the final sensitivity of the system.
} In other words, is the extra $$$ for the larger detector worth it if the work distance is at the same time increased?
}
} Many thanks in advance.
} Stephane
}
}
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From: wesaia-at-iastate.edu
Date: Thu, 20 Feb 2020 09:53:12 -0600
Subject: [Microscopy] question about EDS in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Let me be sure I understand your question correctly. Your first option of a 30-mm2 detector would
allow a 10-mm working distance. Your second option of a 150mm2 detector would require a 15-mm
working distance to the pole piece.
That does not say anything about the sample to detector distance which is required to calculate the
solid angle. The 15-mm working distance might be your better option.
That is similar to the situation we ran into. I wished I had posed the question to the list 9 years
ago. - We had been using a 10mm2 detector on a Hitachi 2460N which was setup for a 25-mm working
distance for the eucentric height and analytical distance. We were able to bring the EDS detector in
below the pole piece so that it was quite close to the sample and gave us a pretty good solid angle.
It did mean that the EDS detector was the first thing in harm's way. A user scratched up one of our
aluminum sample platens when they raised it into the end of the detector then proceeded to move the
sample around. - We upgraded to a FEI Quanta with field emission. It's eucentric height was 10-mm
which was better for imaging. We knew we wanted to get a large detector in order to maintain as much
image resolution as possible when doing EDS so we opted for an 80-mm2 detector figuring we would get
8x more counts at the same beam current. It turned out that we only got about 3x more. We had
ordered the system configured to work at 10-mm working distance. With the design of the pole piece,
that meant our detector had to stand about 70% further off than before. So we gained 8x from the
detector area but lost almost 3x due to the greater distance.
I have wondered about redoing our system to operate at 15-mm working distance and bring the detector
in much closer. It would greatly improve our solid angle. If I was only doing EDS, I would probably
go for it. It would mean different operating conditions for EDS versus just imaging. That might
confuse some of our many users. We also have WDS installed, and I understand it is aimed at 10-mm.
So, I will probably just leave things as they are and bring up the current a bit more.
Now to the other question of performance and resolution as a function of size. It is true that all
other things being equal, you may give up a little energy resolution by going to a bigger detector.
It is harder to find a crystal of the same, good resolution when it has to be 8x larger, but it can
be done. They will probably charge a premium for it. You probably want to compare spectra on your
real samples.
I choose to back off on the process time to push more counts at the same deadtime. So, I am already
sacrificing some resolution. I am glad that I started with a better detector. There are times that I
want for better resolution so I increase the process time and cut the count rate - or I switch over
to the WDS.
Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
X-from: nizets2-at-yahoo.com {nizets2-at-yahoo.com} Sent: Wednesday, February 19, 2020 4:41 PM
To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

Dear colleagues,

I'd have a simple question regarding the detector area of EDX (EDS) systems in SEM:
Clearly the largest the detector, the highest the sensitivity for everything else equal but what if
everything else was NOT equal?

One clear example:

- detector 1: 30mm², optimal distance to pole piece 10mm
- detector 2: 150mm², optimal distance to pole piece 15mm

Here the largest detector is also the furthest!

I don't know how to appreciate the influence of the 2 factors, detector area and work distance, on
the final sensitivity of the system.
In other words, is the extra $$$ for the larger detector worth it if the work distance is at the
same time increased?

Many thanks in advance.
Stephane

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From: microscopy.listserver-at-gmail.com
Date: Fri, 21 Feb 2020 16:24:35 -0600
Subject: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM sample preparation

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Email: arunas.mesceriakovas-at-uef.fi Name: Arnas Meeriakovas

Organization: University of Eastern Finland

Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM sample preparation

Message: Hello,


I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use TEM to check the
morphology/size distribution of the sample i.e. individual particles or agglomerates. I have
prepared the samples by dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.

I am aware that due to drying of the solvent from the TEM grid, the carbon dots that are present in
the drop are forced together and may form agglomerates.
I would like to eliminate/minimize the agglomerate formation in the sample preparation process as it
is influencing my analysis which is used to evaluate the synthesis method (formation of single
particles/formation of agglomerates).

What are the possible methods I could use in my sample prep, to get the least possible particle
agglomeration?

Login Host: 193.167.228.180
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From: Frank Karl :      frank_karl-at-ardl.com
Date: Mon, 24 Feb 2020 20:43:06 -0500
Subject: RE: [Microscopy] Fwd: Traceable Standard Specimen for TEM calibration

Contents Retrieved from Microscopy Listserver Archives
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We searched and after several years the answer was clearly no.
We settled our traceable TEM calibration problem by calibrating with a replica grating and then averaging the measurement of 200 particles of a NIST traceable nanospheres. We selected the 200nm (actually203nm) traceable spheres and typically get numbers within 5%. There are smaller traceable spheres, but the 200 have the smallest Std deviation and expanded uncertainty,

Please let me know if you find a better solution.


Stay safe..

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



Orignal Message:


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X-from: Erico Freitas {ericotadeu-at-ufmg.br}


Dear all,


We are looking after a traceable standard specimen for TEM calibration.
We had a MAG*I*CAL sample that got damaged, and it is in a temporary backorder at the EMSDiasum website.

Does any of you know and have used another traceable standard specimen for TEM calibration?


Thank you,

--
Erico Freitas

Physicist/Microscopist at Center of Microscopy
Universidade Federal de Minas Gerais (UFMG)
Av. Antnio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil.
ZIP Code 31270-901.
+55-31-3409-7573
+55-31-3409-7575

Coordinator:Transmission Electron Microscopy laboratory

CV Lattes: *http://lattes.cnpq.br/8786127123101199*
{https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#}



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From: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday,
Date: Mon, 24 Feb 2020 14:52:45 +0000
Subject: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM sample preparation

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Email: arunas.mesceriakovas-at-uef.fi
Name: Arnas Meeriakovas

Organization: University of Eastern Finland

Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM sample preparation

Message: Hello,


I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use TEM to check the
morphology/size distribution of the sample i.e. individual particles or agglomerates. I have
prepared the samples by dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.

I am aware that due to drying of the solvent from the TEM grid, the carbon dots that are present in
the drop are forced together and may form agglomerates.
I would like to eliminate/minimize the agglomerate formation in the sample preparation process as it
is influencing my analysis which is used to evaluate the synthesis method (formation of single
particles/formation of agglomerates).

What are the possible methods I could use in my sample prep, to get the least possible particle
agglomeration?

Login Host: 193.167.228.180
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


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From: mdelann1-at-jhmi.edu
Date: Tue, 25 Feb 2020 12:13:12 -0600
Subject: [Microscopy] UA

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Arunas,
I forgot to mention UA is incompatible with phosphate and will precipitate in its presence. If you
use it then briefly rinse in DH2O before the negative staining (after sample adsorbtion).
Good Luck,
Michael Delannoy
Johns Hopkins SOM Microscope Facility

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From: petereaton-at-hotmail.com
Date: Wed, 26 Feb 2020 05:21:41 -0600
Subject: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
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Dear Arunas,
You probably already considered this, but whatever you see on TEM is not going to be representative
of what's happening in solution, irrespective of preparation method. (Except, maybe
freezing/Cryo_TEM?). If what you want to know is how they are dispersed in solution, then DLS or a
similar light scattering technique might be best. 20nm particles are well within the size range
that's possible. Although, I suppose there will be relatively little contrast due to the material,
it should be feasible. In any case, it's a very quick method to try.
Good luck,
Pete.
____________________________________________________________________________
Atomic Force Microscopy
Dr Peter Eaton and Dr Paul West
Available through all good bookshops, or direct from Oxford University Press at:
http://ukcatalogue.oup.com/product/9780199570454.do http://afmhelp.com


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Email: arunas.mesceriakovas-at-uef.fi Name: Arnas Meeriakovas

Organization: University of Eastern Finland

Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM
sample preparation

Message: Hello,


I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use
TEM to check the morphology/size distribution of the sample i.e.
individual particles or agglomerates. I have prepared the samples by
dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.

I am aware that due to drying of the solvent from the TEM grid, the
carbon dots that are present in the drop are forced together and may
form agglomerates.
I would like to eliminate/minimize the agglomerate formation in the
sample preparation process as it is influencing my analysis which is
used to evaluate the synthesis method (formation of single
particles/formation of agglomerates).

What are the possible methods I could use in my sample prep, to get the
least possible particle agglomeration?

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23, 77 -- sample preparation
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From: vray-at-partbeamsystech.com
Date: Wed, 26 Feb 2020 08:43:45 -0600
Subject: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Look up "Ammonium Laurate Surfactant for Cleaner Deposition of Carbon Nanotubes" by Hannah Nisson,
DOI:10.1021/acs.langmuir.5b01175 on using surfactants to avoid agglomeration during TEM sample prep.

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 2/26/2020 6:21 AM, petereaton-at-hotmail.com wrote:
} ----------------------------------------------------------------------------
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} Dear Arunas,
} You probably already considered this, but whatever you see on TEM is not going to be representative of what's happening in solution, irrespective of preparation method. (Except, maybe freezing/Cryo_TEM?). If what you want to know is how they are dispersed in solution, then DLS or a similar light scattering technique might be best. 20nm particles are well within the size range that's possible. Although, I suppose there will be relatively little contrast due to the material, it should be feasible. In any case, it's a very quick method to try.
} Good luck,
} Pete.
} ____________________________________________________________________________
} Atomic Force Microscopy
} Dr Peter Eaton and Dr Paul West
} Available through all good bookshops, or direct from Oxford University Press at:
} http://ukcatalogue.oup.com/product/9780199570454.do http://afmhelp.com
}
}
} ________________________________________
} X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
} Sent: 21 February 2020 22:25
} To: petereaton-at-hotmail.com
} Subject: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM sample preparation
}
}
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}
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} Email: arunas.mesceriakovas-at-uef.fi Name: Arûnas Meðèeriakovas
}
} Organization: University of Eastern Finland
}
} Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM
} sample preparation
}
} Message: Hello,
}
}
} I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use
} TEM to check the morphology/size distribution of the sample i.e.
} individual particles or agglomerates. I have prepared the samples by
} dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.
}
} I am aware that due to drying of the solvent from the TEM grid, the
} carbon dots that are present in the drop are forced together and may
} form agglomerates.
} I would like to eliminate/minimize the agglomerate formation in the
} sample preparation process as it is influencing my analysis which is
} used to evaluate the synthesis method (formation of single
} particles/formation of agglomerates).
}
} What are the possible methods I could use in my sample prep, to get the
} least possible particle agglomeration?
}
} Login Host: 193.167.228.180
} Listserver Email Form V - 20120416
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} 23, 77 -- From: Peter Eaton {petereaton-at-hotmail.com}
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} 23, 77 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} 23, 77 -- Subject: Re: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM
} 23, 77 -- sample preparation
} 23, 77 -- Thread-Topic: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM
} 23, 77 -- sample preparation
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3, 45 -- Subject: Re: [Microscopy] Re: viaWWW:Avoiding nanoparticle agglomeration in
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From: microscopy.listserver-at-gmail.com
Date: Thu, 27 Feb 2020 00:41:53 -0600
Subject: [Microscopy] viaWWW:EM Lab Manager position at CVM Virginia Tech

Contents Retrieved from Microscopy Listserver Archives
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X-from: hancocksk-at-vt.edu

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Email: hancocksk-at-vt.edu

Name: Sandy Hancock

Organization: College of Veterinary Medicine, Virginia Tech

Title-Subject: [Filtered] EM Lab Manager position at CVM Virginia Tech

Message: Hello EM Colleagues,

The Electron Microscopy Laboratory at the College of Veterinary Medicine at Virginia Tech has a
position open for a Lab Manager. The position is responsible for the day-to-day operations of the
service laboratory, which primarily supports researchers on campus who submit biological samples,
with the occasional nanoparticle/materials sample. The laboratory houses a JEOL JEM-1400 TEM, Zeiss
EVO 40 SEM and supporting equipment for specimen preparation. More information can be found at the
position listing http://careers.pageuppeople.com/968/cw/en-us/job/512843/electron-microscopy-lab-mgr

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Sandy

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From: microscopy.listserver-at-gmail.com
Date: Thu, 27 Feb 2020 15:36:13 -0600
Subject: [Microscopy] viaWWW:Buffers other than PBS to use for IEM

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Email: ralvaradojr-at-ufl.edu
Name: Rudy
Organization: University of Florida

Title-Subject: [Filtered] Buffers other than PBS to use for IEM

Message: Hi everyone,
I am trying to process a strain of Lactobacillus johnsonii bacterial cells for immunoelectron
microscopy. These cells hate PBS. My question is: is there other buffers I can use to process this
sample that don't interfere with antigenicity?
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From: microscopy.listserver-at-gmail.com
Date: Thu, 27 Feb 2020 18:32:12 -0600
Subject: [Microscopy] Fwd: Re: viaWWW:Buffers other than PBS to use for IEM

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X-from: shakeel waqqar {shakeelwaqqar-at-gmail.com}


Try Normal Saline

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Email: ralvaradojr-at-ufl.edu {mailto:ralvaradojr-at-ufl.edu}
Name: Rudy
Organization: University of Florida

Title-Subject: [Filtered] Buffers other than PBS to use for IEM

Message: Hi everyone,
I am trying to process a strain of Lactobacillus johnsonii bacterial
cells for immunoelectron microscopy. These cells hate PBS. My question
is: is there other buffers I can use to process this sample that don't
interfere with antigenicity?
Thanks
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From: Geoffrey McAuliffe :      mcauliff-at-rwjms.rutgers.edu
Date: Thu, 27 Feb 2020 18:32:12 -0600
Subject: Re: [Microscopy] viaWWW:Buffers other than PBS to use for IEM

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PIPES or HEPES

Geoff

**********************************************
Geoff McAuliffe, Ph.D.
Assistant Professor
Director, Electron Microscopy Core
Department of Neuroscience and Cell Biology
Rutgers, Robert Wood Johnson Medical School
675 Hoes Lane West, Piscataway, NJ 08854
voice: (732) 235-4583
mcauliff-at-rwjms.rutgers.edu
**********************************************

==============================Original Headers==============================

==============================End of - Headers==============================






From: Peter van de Plas :      p.vandeplas-at-aurion.nl
Date: Mon, 2 Mar 2020 13:55:40 +0000
Subject: Re: [Microscopy] viaWWW:Buffers other than PBS to use for IEM

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Dear Rudy,

I suggest to process in a buffer that is compatible with your bacterial strain.
Buffers in general will not have a negative effect on antigenicity. I mainly used 100mM PB and PHEM
buffers in the past, when processing cells in culture.

Are you going to chemically fix your specimen with aldehydes?
Do you intend to embed in a plastic?
Choose/work out a fixation method and embedding method compatible with the characteristics of the
primary antibody.
You may test the effect of fixation on signal intensity already on the LM level using an
immunofluorescent method or combine immunogold labeling with silver enhancement.

During the actual immuno incubation it will be beneficial to use a buffer with sodium chloride.
In general PBS, pH 7.4 is a good option. TBS is an alternative. The buffer capacity of Tris at pH
7.4 is however suboptimal. You may go up to a higher pH, e.g., pH 8.2 depending on the additional
additives you use to diminish background staining.
I recommend
https://aurion.nl/sharing-our-knowledge/newsletters-and-newsflyers/newsletter-1-background-suppression/for
additional information.

Kind regards,

Peter van de Plas





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} Title-Subject: [Filtered] Buffers other than PBS to use for IEM
}
} Message: Hi everyone,
} I am trying to process a strain of Lactobacillus johnsonii bacterial
} cells for immunoelectron microscopy. These cells hate PBS. My question
} is: is there other buffers I can use to process this sample that don't
} interfere with antigenicity?
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Peter van de Plas
Aurion
Binnenhaven 5
Wageningen
6709 PD The Netherlands
Phone: +31 317 415094
http://www.aurion.nl







From: microscopy.listserver-at-gmail.com
Date: Thu, 5 Mar 2020 18:47:20 -0600
Subject: [Microscopy] viaWWW:Printing tiff images

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Name: Rick Van Camp

Organization: Kettering University

Title-Subject: [Filtered] Printing tiff images

Message: I would like to display some of the tiff images that have been collected with our TEM in
the hall outside the lab. Can anyone advise me on what properties a given printer needs to possess
such that these images retain the high quality they inherently possess? The idea is to leave a
positive impression on those people that walk by the lab.

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From: wij.muss-at-aon.at
Date: Thu, 5 Mar 2020 19:19:40 -0600
Subject: [Microscopy] Re: Printing tiff images

Contents Retrieved from Microscopy Listserver Archives
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Dear Rick,
Unfortunately I don’t have practical experience with PINTING TIFF files properly…don’t own a
TIFF-printable printer (☺)


But: Information is available:
e.g. https://itstillworks.com/print-large-tiff-file-6469452.html

Copy & Paste: Just for convenience of all Readers/colleagues eventually not being able to access the
given URL:
QUOTE: How to Print a Large Tiff File
Step 1
Select a paper that is suited to print photographic images. Using plain typing paper or copy paper
will not capture the range of values and colors inherent in a high-quality Tiff file. If possible,
select the same type of paper as the manufacturer of your printer.
Step 2
Access a printer that is designed to Tiff quality images, if possible. Most home inkjet printers
will print a photographic image of an acceptable quality, though photo-specific printers will give
the contrast and color range that a Tiff is designed to provide. These types of printers often have
the word "photo" in their model name, and use individual-color ink cartridges.
Step 3
Clean the print heads on your printer. This prevents lines, known as banding, as well as color
shifts. Right-click on the printer icon in the taskbar, located on the lower right corner of your
screen. Follow the step-by-step instructions after clicking the option to clean the print heads.
Step 4
Open the Tiff file in your computer's imaging software or default picture viewer. Make any changes
to contrast, color and cropping as necessary or possible.
Step 5
Select Print from the File menu in the software. Select the option called Page Options or Print
Options, depending on your software model. This will bring up a dialog box specific to your printer.
Step 6
Choose the paper size, orientation, the source. The size will likely be legal, or 8.5 by 11 inches.
The orientation refers to whether the image is tall (portrait) or long (landscape). The source is
either a sheet or roll of paper.
Step 7
Select the type of media being used. The instruction sheet with your paper may suggest a certain
media based on your printer manufacturer. There may be a number of options to experiment with, but
the important detail is to select whether the paper you have chosen has a glossy surface or is matte.
Step 8
Select the highest print quality available. This option is known under various terms depending on
the manufacturer, but will usually be the top or last quality in a list of options.
Load the paper into the printer on its correct side as explained by the paper's instruction sheet.
Press print. If the print has problems with its contrast or color, return to the file in the imaging
software. Make the necessary changes and repeat the process as needed.
Tip
• So as not to waste ink, use the selector tool in your imaging software to choose a portion of
the image with a variety of colors or contrast. Copy and paste this portion into a new document.
Make the necessary changes and print test strips until it is determined what changes to apply to the
entire image. Items you will need
• Computer with imaging software or default picture viewer
• Tiff file
• Printer, preferably of photographic quality

Elizabeth MOTT,
My Printer Is Not Printing TIFF Files
https://smallbusiness.chron.com/printer-not-printing-tiff-files-57302.html
[ Related Articles,
References (3)
https://lbis.kenyon.edu/helpline/printers/troubleshooting
Microsoft: Description of the Guidelines for Selecting the Appropriate Picture Format in an
Office Program
Adobe Systems: Developer Resources: TIFF
Resources (1)
Dux Computer Digest: How to Troubleshoot Printer Problems ]
What Is the Best Image Format for Printing?
https://www.shutterstock.com/support/article/best-image-format-for-printing

Asked Feb 2019:
https://photo.stackexchange.com/questions/104804/jpeg-or-tiff-files-better-for-print-images
JPEG or TIFF files better for print images?

Asked August 2007
https://www.photo.net/discuss/threads/is-it-possible-to-print-tiff-files.283228/

Best regards and good luck,

Wolfgang MUSS
SALZBURG, AUSTRIA

=========================================================
MUSS Wolfgang Dr. phil. / PhD - retired
Ignaz-Rieder-Kai 19/6
A-5020 SALZBURG
Österreich-AUSTRIA
Mobile-Tel.: 0043(0)676 5 369 456
E-mail: wij.muss-at-aon.at
E-Mail altern.: womuss-at-gmail.com
FRMS, Retired Member of MSA & other (Inter-)National Societies
Former Head of Electron Microscopy Lab at Institute of Pathology
SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL
UNIVERSITY of SALZBURG
Scientific Profile at ResearchGate:
http://www.researchgate.net/profile/Wolfgang_MUSS
inviting you to join RG (Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html,
and join 15+ million researchers, including 63 Nobel Laureates)
„ResearchGate is the professional network for scientists and researchers. Over 15 million members
from all over the world use it to share, discover, and discuss research. We're guided by our mission
to connect the world of science and make research open to all.“

===================================================================================
Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Freitag, 6.
März 2020 01:48
An: wij.muss-at-aon.at
Betreff: [Microscopy] Printing tiff images

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From: julietran.tsuki-at-gmail.com
Date: Thu, 5 Mar 2020 18:02:45 -0800
Subject: Re: [Microscopy] viaWWW:Printing tiff images

Contents Retrieved from Microscopy Listserver Archives
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Hello Rick

If you are interested in printing out your images with your printer. Make sure that your printer has
the capabilities to print on photo paper and what type of ink it uses. If you check the name/version
of your printer you can look up to see what your printer can and cannot do for printing qualities.
When you do print be sure to make sure that the file format/ size of image you have. Due to when you
print out your image and you manipulate it may warp the image.  Here are some links to help you.
-Julie

Link best printer for photos
} }
https://www.bestproducts.com/tech/electronics/a26933257/top-rated-photo-printers/?src=arb_ga_bp_m_bm_a26933257&utm_source=google&utm_medium=cpc&utm_campaign=arb_ga_bp_m_bm_a26933257&gclid=EAIaIQobChMIgPyip-CE6AIVkspkCh1Mowy8EAAYASAAEgJGa_D_BwE

Why do pictures look different when I print them link
} }
https://www.google.com/amp/s/www.howtogeek.com/397798/why-do-photos-look-different-when-i-print-them/amp/

Sent from my iPhone

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} Title-Subject: [Filtered] Printing tiff images
}
} Message: I would like to display some of the tiff images that have been collected with our TEM in
} the hall outside the lab.  Can anyone advise me on what properties a given printer needs to possess
} such that these images retain the high quality they inherently possess?  The idea is to leave a
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From: oshel1pe-at-cmich.edu
Date: Fri, 6 Mar 2020 08:26:07 -0600
Subject: [Microscopy] Re: viaWWW:Printing tiff images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Rick,

1) Epson Photo printer with Photo inks, 6 to 9 color (the blue ink is used in B&W printing). But!
Don't buy one unless you will be printing at least once a month, maybe even once a week. Inks are
hideously expensive and 3rd party inks don't work well or at all (inks are bar coded so a printer
company can prevent other brands or refills from being used). However. Unless you do print
something, even a test page, every so often, the ink dries, clogs a valve or three, and this cannot
be fixed. The printer is trash.
So, if you just want a set of images for the hallway, use the campus printing service or a business.

2) High quality photo paper. Let the ink completely dry (it sits on top of the coating, hence higher
resolution printing) for 30-60 minutes. *Laminate* the print. This seals out oxygen, which fades images.

3) When moving the image files, do *not* use drag-and-drop, use copy/paste. Drag and drop in Windows
can change the image resolution to the monitor's resolution. This was true, I do not know if it is
still true with Windows 10 (or 7), but I don't trust it. I don't know about Linux, and I don't think
MacOS does this.
But copy/paste is easy.

4) Not what you asked, but more of a tech-looking thing that makes admin types happy: Put up a
display monitor or three in the hallway, and set up a computer to send images to the monitors. No
printer, no buying expensive inks, no worrying about file types and sizes, easier to change images,
etc. You can maybe get the monitors and computer from campus IT - ones that have been replaced with
something newer, but are still perfectly good and likely free.

Phil
------------- Philip Oshel Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office

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Email: rvancamp-at-kettering.edu
Name: Rick Van Camp
Organization: Kettering University
Title-Subject: [Filtered] Printing tiff images
Message: I would like to display some of the tiff images that have been collected with our
TEM in the hall outside the lab. Can anyone advise me on what properties a given printer needs
to possess such that these images retain the high quality they inherently possess? The idea is
to leave a positive impression on those people that walk by the lab.


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From: microscopy.listserver-at-gmail.com
Date: Sat, 7 Mar 2020 12:45:41 -0600
Subject: [Microscopy] Fwd: Microscopy-microanalysis technician position

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Harland, Duane {Duane.Harland-at-agresearch.co.nz}


Dear colleagues,

We have a job vacancy for a permanent position, part time, technician in our
microscopy/microanalysis laboratory here at AgResearch Institute in New Zealand.

We are primarily working on biological and bio-based materials using SEM, TEM and other similar
methods. We are looking to hire a technician to carry out a range of tasks from assisting with
sample preparation to helping look after the instrumentation and do some troubleshooting etc. The
role would suit someone with a good working knowledge with instrumentation, an eye for detail and
good collaboration skills.

For more details look under AgResearch on the Science New Zealand website under careers for more
details.

https://careers.sciencenewzealand.org/agresearch-jobdetails/ajid/xcR09/Microscopy-and-Microanalysis-Technician,37409.html

Duane

*AgResearch Limited | *Lincoln Research Centre *| New Zealand*
Dr Duane P Harland, Senior Scientist
*T*+64 3 321 8710 *E*duane.harland-at-agresearch.co.nz {mailto:duane.harland-at-agresearch.co.nz}

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From: microscopy.listserver-at-gmail.com
Date: Sat, 7 Mar 2020 12:48:16 -0600
Subject: [Microscopy] viaWWW:We are hiring! Electron Microscopist / Imaging Specialist

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X-from: mrajasingham-at-sivananthanlabs.us

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Email: mrajasingham-at-sivananthanlabs.us Name: Madhura Rajasingham

Organization: Sivananthan Laboratories, Inc.

Title-Subject: [Filtered] We are hiring! Electron Microscopist / Imaging Specialist

Message: Hello!
Sivananthan Laboratories, Inc. is a high-tech business incubator focused on promoting economic
growth in Illinois and the United States through fostering cutting-edge, fundamental research and
development. We work on R&D for AI, infrared detectors, image processing, solar energy, and more.
We are currently looking for a successful candidate who is skilled in all aspects on scanning
electron microscopy (SEM), transmission electron microscopy (TEM) and scanning transmission electron
microscopy (STEM) with experience in image simulation and state-of-the-art processing and analytics
packages. If you are interested, please go to this link (http://sivananthanlabs.us/employment/) and
apply for this position!

If you have any questions, feel free to email me at mrajasingham-at-sivananthanlabs.us

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From: microscopy.listserver-at-gmail.com
Date: Sat, 7 Mar 2020 14:52:56 -0600
Subject: [Microscopy] Fwd: LaB6 on FEI BioTwin12

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Ning, Gang {gxn7-at-psu.edu}

Dear Listers,

One of my users wants to replace a tungsten filament with aLaB6tip in my FEI BioTwin12 for
screening/imaging cryoEM samples. I know aLaB6is much more expensive. But regardless the cost, what
are other downside impact on my other users who primarily use the scope for imaging plastic
sections, negative-stained samples? Thank you!

Best regards,

Greg

Gang (Greg) Ning

Microscopy Facility

Huck Institutes of the Life Sciences

Penn State University

MSC N-048

University Park, PA 16802

814-863-0994

https://www.huck.psu.edu/core-facilities/microscopy-facility



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From: Erico Freitas :      ericotadeu-at-ufmg.br
Date: Sat, 7 Mar 2020 18:27:00 -0300
Subject: Re: [Microscopy] Fwd: LaB6 on FEI BioTwin12

Contents Retrieved from Microscopy Listserver Archives
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Hi Greg,


We're about to the same in our Tecnai Spirit Biotwin T12. The FEI/Thermofisher guys have no concern
about that.

I've used a T12 in CMM at the University of Queensland, Brisbane/Australia that has got a LaB6.

You must clean the gun assembly and the Wehnelt as well in order to get rid of W residues before.

Although a LaB6 is more expensive than a W filament, its life time is much longer. It might last
over 1000 ours. It lasts 2000 hours in average on our Tecnai T20. You know it will give you a
brighter beam, and its energy spread is lower.

Regarding the other users, they will be quite happy when looking at their plastic sections and
negative staining, not only the cryo-EM users.

It's always a good practice to set a lower C2 aperture and play around with the spot size to a
suitable electron dose for each sample. I know some users don't like to change the apertures and do
the alignments but they should get use of it.

My only concern is about the gun preassure. As the Tecnai Spirit Biotwin T12 does have a dedicated
IGP for the gun (ours doesn't have), the vacuum in the gun is broken down every time you run the
cryo cycle. So the LaB6 cathode gets contaminated. But I still think the benefits of the LaB6 will
give you are worth to have it on your T12.


Best wishes,


On Sat, 7 Mar 2020, 16:56 , {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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X-from:         Ning, Gang {gxn7-at-psu.edu {mailto:gxn7-at-psu.edu} }

Dear Listers,

One of my users wants to replace a tungsten filament with aLaB6tip in my FEI BioTwin12 for
screening/imaging cryoEM samples. I know aLaB6is much more expensive. But regardless the cost, what
are other downside impact on my other users who primarily use the scope for imaging plastic
sections, negative-stained samples?    Thank you!

Best regards,

Greg

Gang (Greg) Ning

Microscopy Facility

Huck Institutes of the Life Sciences

Penn State University

MSC N-048

University Park, PA 16802

814-863-0994

https://www.huck.psu.edu/core-facilities/microscopy-facility




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From: microscopy.listserver-at-gmail.com
Date: Sat, 7 Mar 2020 16:20:25 -0600
Subject: [Microscopy] Re: Fwd: LaB6 on FEI BioTwin12

Contents Retrieved from Microscopy Listserver Archives
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Hello Greg,

if your FEI TEM is equipped with a getter pump and does reach a sufficient vacuum value you will
generally benefit from switching to LaB6 cathodes. An LaB6 cathode can be pre-mounted in a spare
wehnelt and interchanged within an hour, if the vacuum system in the rest of the scope is pumped
down to limit. Use dry Nitrogen gas for flooding and let it run until cathode / wehnelt is
changed... LaB6 cathode needs to be centred VERY well, within a few 1/100 mm. For accurate height
settings see the data sheets at Kimball or DENKA websites.

The spot diameter will be smaller than with Tungsten, the electrons coming from the cathode more
monochromatic. Both things will help getting better resolution / better focus and astigmatism
corrections in the images.

The luminosity of the gun will be significantly higher; that would help at magnifications more than
50-100 k... But: very thin UM cuts could burn under a concentrated beam.

I suppose the automatic heating regime for the LaB6 cathode could be selected at the TEM setup. It
will need slow heating (and cooling), ca. 2 minutes each.

Other than the cathode handling and the costs (but: cathode will run ca. 1000-2000 hours) there is
only win-win...

I use LaB6 since a lot of years at a Philips / FEI EM420.


Best,

Stefan


-----------------------------------------------------
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} X-from: Ning, Gang {gxn7-at-psu.edu}
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} Dear Listers,
}
} One of my users wants to replace a tungsten filament with aLaB6tip in my FEI BioTwin12 for
} screening/imaging cryoEM samples. I know aLaB6is much more expensive. But regardless the cost, what
} are other downside impact on my other users who primarily use the scope for imaging plastic
} sections, negative-stained samples? Thank you!
}
} Best regards,
}
} Greg
}
} Gang (Greg) Ning
}
} Microscopy Facility
}
} Huck Institutes of the Life Sciences
}
} Penn State University
}
} MSC N-048
}
} University Park, PA 16802
}
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} https://www.huck.psu.edu/core-facilities/microscopy-facility
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From: microscopy.listserver-at-gmail.com
Date: Sun, 8 Mar 2020 16:39:49 -0500
Subject: [Microscopy] Administrivia: Sorry all this is just a system test - Your Friendly

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From: microscopy.listserver-at-gmail.com
Date: Mon, 9 Mar 2020 15:01:55 -0500
Subject: [Microscopy] viaWWW: lead aspartate as post-stain

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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] lead aspartate as post-stain

Message: Hello everyone,
I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate).
Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep.
If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate
any and all information.

THanks!
John Shields
Univ. of Georgia

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From: microscopy.listserver-at-gmail.com
Date: Mon, 9 Mar 2020 18:12:53 -0500
Subject: [Microscopy] viaWWW: lead aspartate as post-stain

Contents Retrieved from Microscopy Listserver Archives
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

John,

Lead aspartate? Isn't that used as a sweetner in some of the heavier diet pastries?

Phil
-----------------------------------------
Philip Oshel
Imaging Center Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576 office
(989) 774-7567 lab



________________________________________
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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] lead aspartate as post-stain

Message: Hello everyone,
I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate).
Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep.
If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate
any and all information.

THanks!
John Shields
Univ. of Georgia

Login Host: 198.137.20.67
Listserver Email Form V - 20120416
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From: microscopy.listserver-at-gmail.com
Date: Wed, 11 Mar 2020 09:01:26 -0500
Subject: [Microscopy] viaWWW: lead aspartate as post-stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Frank Karl {frank_karl-at-ardl.com}


No no no, you're think of sugar of lead or lead acetate. Hey, it worked for the Roman Empire.




Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, March
9, 2020 7:19 PM
To: Frank Karl {frank_karl-at-ardl.com}

X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

John,

Lead aspartate? Isn't that used as a sweetner in some of the heavier diet pastries?

Phil
-----------------------------------------
Philip Oshel
Imaging Center Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576 office
(989) 774-7567 lab



________________________________________
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Sent: Monday, March 9, 2020 16:08
To: Oshel, Philip Eugene

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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at
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---------------------------------------------------------------------------
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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] lead aspartate as post-stain

Message: Hello everyone,
I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate).
Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep.
If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate
any and all information.

THanks!
John Shields
Univ. of Georgia

Login Host: 198.137.20.67
Listserver Email Form V - 20120416
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From: John Shields :      johnshields59-at-gmail.com
Date: Wed, 11 Mar 2020 15:42:25 -0400
Subject: Re: [Microscopy] Fwd: RE: Fwd: Re: viaWWW: lead aspartate as post-stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Oh Right, right,
Sorry,  I mean the "Stain formerly known as Lead Aspartame".
Sheesh
John S

On Wed, Mar 11, 2020 at 9:04 AM {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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X-from: Frank Karl {frank_karl-at-ardl.com {mailto:frank_karl-at-ardl.com} }


No no no,  you're think of sugar of lead or lead acetate.   Hey, it worked for the Roman Empire.




Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio  44305

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Subject: [Microscopy] Fwd: Re: viaWWW: lead aspartate as post-stain




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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }

John,

Lead aspartate? Isn't that used as a sweetner in some of the heavier diet pastries?

Phil
-----------------------------------------
Philip Oshel
Imaging Center Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576 office
(989) 774-7567 lab



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Subject: [Microscopy] viaWWW: lead aspartate as post-stain




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Email: jpshield-at-uga.edu {mailto:jpshield-at-uga.edu} Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] lead aspartate as post-stain

Message: Hello everyone,
I have been searching for any work on lead aspartate used as a post-stain (similar to lead
citrate).
Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample
prep.
If anyone has literature, or has done some previous work seeing if LA would work, I would
appreciate
any and all information.

THanks!
John Shields
Univ. of Georgia

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From: microscopy.listserver-at-gmail.com
Date: Thu, 12 Mar 2020 09:17:16 -0500
Subject: [Microscopy] viaWWW: High Vacuum STM available

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Email: mbrukman-at-seas.upenn.edu Name: Matthew Brukman

Organization: University of Pennsylvania

Title-Subject: [Filtered] High Vacuum STM available

Message: Good morning!

We have a surplus high vacuum STM system free to a good home. Components include

RHK controller
Omicron STM-1 head
vacuum chamber
PC with RHK software
roughing, turbo, and ion pumps
frame with air-supported legs

Recipient would be responsible for transport and reassembly. Please contact me with questions.

Regards,
Matt


Matthew Brukman
Scanning and Local Probe Facilities Director
Singh Center for Nanotechnology
3205 Walnut St. Phila., PA 19104
215-746-2373

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From: Alan.Paris-at-leica-microsystems.com
Date: Fri, 13 Mar 2020 08:40:56 -0500
Subject: [Microscopy] LM: Recommended Materials/Procedure for Disinfecting Optical

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Is there a recommended procedure /materials for disinfecting optical microscope eyepieces, optics
and surfaces against COVID-19?

Alan Paris
Leica Microsystems Inc.
alan.paris-at-leica-microsystems.com









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From: microscopy.listserver-at-gmail.com
Date: Mon, 16 Mar 2020 17:34:03 -0500
Subject: [Microscopy] viaWWW: ] How to clean nickel shim of magnetic and or glass particles

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Email: nmz787-at-gmail.com Name: Nathan McCorkle

Organization: Sole proprietor

Title-Subject: [Filtered] How to clean nickel shim of magnetic and or glass particles?

Message: I have a nickel shim destined for nanoimprint lithography, made by electroforming e-beam
exposed photoresist. I don't have a proper cleanroom, but I've been trying to strip what seemed like
residual ZEP e-beam resist... And it has not been going so well. I've tried acetone,
dichloromethane, n-methyl pyrrolidone, and 10% NaOH. Sonicated with heat in both acetone and NaOH
(at different times). The NaOH is the most recent attempt, and it seemed to show improvement under
FIB imaging, but I also noticed what appeared to be redeposition. I can only imagine this is due to
particulate in my solvents, dirty air as I blow dry the shim or carry it from my sonicator to my FIB
desk, or maybe insoluble particles like glass or ferromagnetic dust which start to settle onto the
sample as soon as the sonicator is turned off.

Features are around 150nm linewidth, high frequency and complex shaped... So lots of small say 500nm
sized holes/crevices which I'd been thinking was just diffusion limited for the solvent to get into
and do it's work. But now I'm pretty confused.

Should I invest in some .45 and .22 micron syringe filters for all my fluid work? Should I tape a
magnet to the outside of the beaker I've been sonicating in, to try and collect such particles?
What's a standard semiconductor lab method for cleaning magnetic particles from magnetic layers? How
about the idea of insolubles? Or can someone recommend a solution that will etch glass but not nickel?

Thanks,
-Nathan

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From: mabon-at-illinois.edu
Date: Tue, 17 Mar 2020 13:07:41 -0500
Subject: [Microscopy] Job Opening: University of Illinois at Urbana-Champaign seeking a

Contents Retrieved from Microscopy Listserver Archives
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Research Scientist Materials Research Laboratory
University of Illinois at Urbana-Champaign

The Materials Research Laboratory (MRL) at the University of Illinois at Urbana-Champaign is seeking
a highly motivated Research Scientist to participate and actively support research in the area of
scanning electron microscopy. The Research Scientist will provide comprehensive technical support
and user training for equipment, procedures, and safety in the broad areas of materials
microanalysis and microfabrication.

The successful candidate will become a member of the dedicated staff of approximately 20 scientists
and engineers, who maintain major research instruments in the MRL's core facilities for electron
microscopy, soft materials characterization, scanning probe microscopy, laser and optical
spectroscopy, surface analysis, x-ray diffraction, and nanofabrication facilities including two
cleanrooms. Approximately 1,000 researchers from across our campus as well as other academic
institutions, industry, and national laboratories use the facilities, logging more than 100,000 user
hours annually. The lab is recognized as one of the premier mid-sized user facilities in the nation.
For details, please visit us online at https://mrl.illinois.edu/facilities/.

The University of Illinois is an Equal Opportunity, Affirmative Action employer. Minorities, women,
veterans and individuals with disabilities are encouraged to apply. For more information, visit
http://go.illinois.edu/EEO.

Responsibilities include:
* Prepare and deliver primary and advanced training on the various techniques and instrumentation
available in the electron microscopy core, particularly in scanning electron microscopy and related
techniques.
* Formulate, compile and distribute suitable suggestions for documentation improvements for
collective staff scientist review. Incorporate approved modifications into training documents and
procedures. This includes the creation of video and multimedia tutorials.
* Actively participate in research using electron microscopy techniques in new materials, such as
ceramics, metals, semiconductor multilayers and super lattices, polymer and biological materials, etc.
* Perform routine preventative maintenance tasks which vary daily, monthly, and annually for
assigned laboratory instrumentation. Examples include daily checks to ensure instruments are running
correctly; monthly proactive checks to ensure no hazards are present; annual maintenance service on
vacuum pumps and supporting mechanical equipment.
* Identify hazards and/or potential failure modes by comparing equipment usage and performance to
establish safety protocols while conducting user training or performing maintenance. If the
equipment is not operating within tolerances or any engineering safety controls are malfunctioning,
determine corrective actions to hardware, operating procedures or user training in conjunction with
the assigned staff scientist and implement the changes.
* Crosstrain in other facility techniques in order to act as backup for other staff members and
increase knowledge and experience.
* Give oral presentations to large audiences, including recording training material for media
outlets, and online, live video streaming.
* Perform facility-wide safety tasks as assigned.
* Conduct department or campus specified lab inspections for assigned operating areas and
participate in reviews of non-assigned areas as requested.
* Assume additional responsibilities to promote the unit's mission as needed.

Minimum Qualifications
* Ph.D. degree in engineering, chemical, physical sciences or related field.
* Two years of hands-on experience in the following areas:
* operation of scanning electron microscopes, including detailed knowledge of main physical
principles, concepts and applications of electron microscopy;
* training researchers in the use of scanning electron microscopes including data interpretation on
related techniques;
* troubleshooting, preventive maintenance and routine repair of scanning electron microscopes;
* use of advanced analytical techniques such as energy-dispersive X-ray spectrometry,
cathodoluminescence imaging and spectrometry, and electron backscatter diffraction using the
scanning electron microscope.
* sample preparation techniques for electron microscopy including polishing, coating, milling, etc.
* Three years of instructional/training experience delivering technical information.
* Previous experience in creating/developing instructional, instrument operation and training
material in electron microscopy.
* Excellent oral and written communication skills.

Preferred Qualifications
* Experience working in a multi-user academic research facility.
* Post-doctoral experience in engineering, chemical, physical sciences or related field.
* Chemistry background necessary for identifying chemicals for waste processing.
* Experience with operation of focused ion beam (FIB/SEM) systems and transmission electron
microscopes and related techniques.

This is a full-time, benefits-eligible academic professional position appointed on a 12-month
service basis. The expected start date is as soon as possible. Salary is commensurate with
experience and qualifications. To apply, please complete a candidate profile at
http://jobs.illinois.edu and upload the following as a single file: a cover letter, resume, and the
names and contact information for three professional references. To ensure full consideration, all
requested information must be submitted by April 2, 2020.

For further information regarding application procedures, contact Summer Redman at
mailto:sredman-at-illinois.edu or 217-300-5400.

The University of Illinois conducts criminal background checks on all job candidates upon acceptance
of a contingent offer. As a qualifying federal contractor, the University of Illinois System uses
E-Verify to verify employment eligibility. The University of Illinois must also comply with
applicable federal export control laws and regulations and, as such, reserves the right to employ
restricted party screening procedures for applicants.


________________________________________
James C Mabon, Ph.D.
Senior Research Scientist
University of Illinois at Urbana-Champaign
Materials Research Laboratory
104 S. Goodwin Ave.
Urbana, Illinois, 61801
________________________________________

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From: microscopy.listserver-at-gmail.com
Date: Sat, 21 Mar 2020 08:42:56 -0500
Subject: [Microscopy] viaWWW: Teaching SEM Online

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Email: Stephen.Beck-at-ncc.edu Name: Steve Beck

Organization: Nassau Community College

Title-Subject: [Filtered] Teaching SEM Online

Message: Dear Colleagues,
I am teaching my SEM course this semester and like many institutions, we are trying to teach
online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online,
however, after that we need to get on the SEM to image the required samples.
Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
this is impossible - I haven't received the courtesy of a response yet!

Thanks!
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From: microscopy.listserver-at-gmail.com
Date: Sat, 21 Mar 2020 09:51:42 -0400
Subject: Re: [Microscopy] viaWWW: Teaching SEM Online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

X-from: lolita.rotkina-at-yandex.com {lolita.rotkina-at-yandex.com}
Envelope-From: lolita-rotkina-at-yandex.ru


Hello Steve!

It is very challenging situation, but everything depends on what generation of hardware you have in
the lab.
Depends on who is your vendor, it may be possible to share the screens and allow users to move the
mouse and type on the screen.
Such things exist for the troubleshooting on most of the modern instruments. Technically, some
instruments can be even used remotely for a high quality work. The only bottleneck I'd - someone
needs to.prepare the sample and mount it inside the analytical tool.
As the instruments get more and more sophisticated and  automated - that approach may become more
popular anyway.

Ask your vendor - they may already have the answer for you.

Stay healthy!

Lolita
8:45 AM, March 21, 2020, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} :




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Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck

Organization: Nassau Community College

Title-Subject: [Filtered] Teaching SEM Online

Message: Dear Colleagues,
I am teaching my SEM course this semester and like many institutions, we are trying to teach
online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam
online,
however, after that we need to get on the SEM to image the required samples.
Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
this is impossible - I haven't received the courtesy of a response yet!

Thanks!
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Lolita Rotkina, PhD

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From: David Huskisson :      david-at-emanalytical.co.uk
Date: Sat, 21 Mar 2020 14:07:31 +0000
Subject: Re: [Microscopy] viaWWW: Teaching SEM Online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


Hi Stephen,

We have a couple of our SEMs connected to the internet for remote access. Through this interface,
the microscope is completely controllable from your own PC. We can load your samples and then you or
your students would be able to take control of the instrument and practice from your own homes.

Let me know how we can help.

Kind regards,

/David Huskisson, PhD./
Project Scientist & Microscopist


Alderley Park
Macclesfield
SK10 4TG

Office: +44 (0) 1625 704 467
DD:     +44 (0) 1625 238 869
Mob:  +44 (0) 7837 718 098

www.emanalytical.co.uk {http://www.emanalytical.co.uk/}


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On Sat, Mar 21, 2020 at 1:48 PM {microscopy.listserver-at-gmail.com
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Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck

Organization: Nassau Community College

Title-Subject: [Filtered] Teaching SEM Online

Message: Dear Colleagues,
I am teaching my SEM course this semester and like many institutions, we are trying to teach
online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam
online,
however, after that we need to get on the SEM to image the required samples.
Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
this is impossible - I haven't received the courtesy of a response yet!

Thanks!
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From: diller-at-stefan-diller.com
Date: Sat, 21 Mar 2020 13:00:11 -0500
Subject: [Microscopy] Re: viaWWW: How to clean nickel shim of magnetic and or

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Nathan,

did you try using a plasma cleaner for cleaning the surfaces and also a plasma cleaner like the
Evactron at the FIB chamber to keep the specimen clean during scanning?

If you can mount the specimen with the surface to be cleaned facing down to the bottom of the beaker
you might get rid of deposits coming from above ;-)

Another try to clean the surfaces might be to plunge it in liquid nitrogen or to use a vacuum
chamber with the cleaning solution and pump to a level below sublimation.

And sure: clean micro-filtered solutions would help.

Nickel and magnetism: you could use a demagnetizer to decrease / erase the magnetism in the shim first.


Best wishes,

Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 21.03.20 um 14:55 schrieb microscopy.listserver-at-gmail.com:
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} Email: nmz787-at-gmail.com Name: Nathan McCorkle
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} Title-Subject: [Filtered] How to clean nickel shim of magnetic and or glass particles?
}
} Message: I have a nickel shim destined for nanoimprint lithography, made by electroforming e-beam
} exposed photoresist. I don't have a proper cleanroom, but I've been trying to strip what seemed like
} residual ZEP e-beam resist... And it has not been going so well. I've tried acetone,
} dichloromethane, n-methyl pyrrolidone, and 10% NaOH. Sonicated with heat in both acetone and NaOH
} (at different times). The NaOH is the most recent attempt, and it seemed to show improvement under
} FIB imaging, but I also noticed what appeared to be redeposition. I can only imagine this is due to
} particulate in my solvents, dirty air as I blow dry the shim or carry it from my sonicator to my FIB
} desk, or maybe insoluble particles like glass or ferromagnetic dust which start to settle onto the
} sample as soon as the sonicator is turned off.
}
} Features are around 150nm linewidth, high frequency and complex shaped... So lots of small say 500nm
} sized holes/crevices which I'd been thinking was just diffusion limited for the solvent to get into
} and do it's work. But now I'm pretty confused.
}
} Should I invest in some .45 and .22 micron syringe filters for all my fluid work? Should I tape a
} magnet to the outside of the beaker I've been sonicating in, to try and collect such particles?
} What's a standard semiconductor lab method for cleaning magnetic particles from magnetic layers? How
} about the idea of insolubles? Or can someone recommend a solution that will etch glass but not nickel?
}
} Thanks,
} -Nathan
}
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From: diller-at-stefan-diller.com
Date: Sat, 21 Mar 2020 13:14:30 -0500
Subject: [Microscopy] Re: viaWWW: Teaching SEM Online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stephen,

if nobody can help you nearby and as long as Corona will stay outside my lab we can try set
something as a life conference from my lab (I might need some help for this, so it might take some
time to set it up...).

I am using a FE-SEM TESCAN MIRA3 and a Philips / FEI TEM EM420.

Some nice resources can be found by searching at google for "teaching electron microscopy"

and here

https://www.fei.com/education-resources/


For some outreach to your students you can use my nanoflight channel at vimeo to show what is
possible with electron microscopy and a lot of effort.

https://vimeo.com/channels/nanoflight

and if you like I can put some more 3D nanoflights online for the OCULUS or other 3D glasses.



Best wishes,

Stefan


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 21.03.20 um 14:52 schrieb microscopy.listserver-at-gmail.com:
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} I am teaching my SEM course this semester and like many institutions, we are trying to teach
} online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online,
} however, after that we need to get on the SEM to image the required samples.
} Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
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}
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18, 29 -- From diller-at-stefan-diller.com Sat Mar 21 13:14:29 2020
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From: vray-at-partbeamsystech.com
Date: Sat, 21 Mar 2020 18:31:40 -0500
Subject: [Microscopy] Re: viaWWW: Teaching SEM Online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Hi Nathan,

To clean a surface of particulates, I would use replicating tape. This is a cellulose acetate tape
(non-adhesive) that you soften with acetone and press down onto the surface. Let it dry and peel it
off. All the particulates should come with it. I've had better luck in removing particulates this
way compared with ultrasonics, rinses, etc.

I'm not sure if an adhesive tape will work but if you don't have replicating tape, you might try
some of the tape with the "Post-it" type adhesive. It may take several applications to remove
everything.
Replicating tape is available from most of the EM supply houses. It comes in both a thick and thin
form.
Cheers,
Henk

----------------


Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Saturday, March 21,
2020 9:53 AM
To: Colijn, Hendrik {colijn.1-at-osu.edu}


Hi Stephen,

Depending on the size of your class - you can use one of remote desktop packages, such as Teamviwer,
VNC, RDP, Chrome remote desktop, etc... to let your students see (and even operate) GUI of the SEM,
and hear your explanations.

I've been using TeamViewer for remote troubleshooting and training of FIB operators a couple of
years, although haven't tried it in "classroom" kind of approach.

Best Wishes,
Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 3/21/2020 9:43 AM, microscopy.listserver-at-gmail.com wrote:
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} Email: Stephen.Beck-at-ncc.edu Name: Steve Beck
}
} Organization: Nassau Community College
}
} Title-Subject: [Filtered] Teaching SEM Online
}
} Message: Dear Colleagues,
} I am teaching my SEM course this semester and like many institutions, we are trying to teach
} online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online,
} however, after that we need to get on the SEM to image the required samples.
} Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
} this is impossible - I haven't received the courtesy of a response yet!
}
} Thanks!
} Login Host: 173.77.159.14
} Listserver Email Form V - 20120416
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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Apr 2020 07:52:48 -0500
Subject: [Microscopy] Fwd: TEM/AFM Researcher position (191486)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html



X-from: David Huskisson {david-at-emanalytical.co.uk}


Hi Stephen,

We have a couple of our SEMs connected to the internet for remote access. Through this interface,
the microscope is completely controllable from your own PC. We can load your samples and then you or
your students would be able to take control of the instrument and practice from your own homes.

Let me know how we can help.

Kind regards,

/David Huskisson, PhD./
Project Scientist & Microscopist


Alderley Park
Macclesfield
SK10 4TG

Office: +44 (0) 1625 704 467
DD:     +44 (0) 1625 238 869
Mob:  +44 (0) 7837 718 098

www.emanalytical.co.uk {http://www.emanalytical.co.uk/}


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On Sat, Mar 21, 2020 at 1:48 PM {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck

Organization: Nassau Community College

Title-Subject: [Filtered] Teaching SEM Online

Message: Dear Colleagues,
I am teaching my SEM course this semester and like many institutions, we are trying to teach
online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam
online,
however, after that we need to get on the SEM to image the required samples.
Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
this is impossible - I haven't received the courtesy of a response yet!

Thanks!
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From kelnanc57ctyee-at-gmail.com Sat Mar 28 20:49:33 2020
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X-from: Erico Freitas {ericotadeu-at-ufmg.br}



Dear all,


I'm just forwarding you the following TEM/AFM Researcher position (191486)
at CNPEM.

The CNPEM is located in Campinas, S=C3=A3o Paulo state, Brazil.

See more details through this link:

https://pages.cnpem.br/trabalheconosco/2020/03/23/researcher-with-a-focus-o=
n-electron-transmission-microscopy-or-atomic-force-191486/
{https://pages.cnpem.br/trabalheconosco/2020/03/23/researcher-with-a-focus-o=n-electron-transmission-microscopy-or-atomic-force-191486/}


This position is likely for the LNNano (Brazilian National Nanotechnology
Laboratory) at CNPEM (https://lnnano.cnpem.br/)


Cheers,

--
Erico Freitas

Physicist/Microscopist at Center of Microscopy
Universidade Federal de Minas Gerais (UFMG)
Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901.
+55-31-3409-7573
+55-31-3409-7575

Coordinator:Transmission Electron Microscopy laboratory

CV Lattes: *http://lattes.cnpq.br/8786127123101199*
{https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#}

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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Apr 2020 08:17:42 -0500
Subject: [Microscopy] Fwd: TEM/AFM Researcher position (191486)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html


X-from: Erico Freitas {ericotadeu-at-ufmg.br}



Dear all,


I'm just forwarding you the following TEM/AFM Researcher position (191486)
at CNPEM.

The CNPEM is located in Campinas, S=C3=A3o Paulo state, Brazil.

See more details through this link:

https://pages.cnpem.br/trabalheconosco/2020/03/23/researcher-with-a-focus-o=
n-electron-transmission-microscopy-or-atomic-force-191486/
{https://pages.cnpem.br/trabalheconosco/2020/03/23/researcher-with-a-focus-o=n-electron-transmission-microscopy-or-atomic-force-191486/}


This position is likely for the LNNano (Brazilian National Nanotechnology
Laboratory) at CNPEM (https://lnnano.cnpem.br/)


Cheers,

--
Erico Freitas

Physicist/Microscopist at Center of Microscopy
Universidade Federal de Minas Gerais (UFMG)
Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901.
+55-31-3409-7573
+55-31-3409-7575

Coordinator:Transmission Electron Microscopy laboratory

CV Lattes: *http://lattes.cnpq.br/8786127123101199*
{https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#}

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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Apr 2020 08:28:30 -0500
Subject: [Microscopy] viaWWW:Aurox On-line Conference on Microscopy 7th and 8th April 2020

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Email: phillipa.timmins-at-aurox.co.uk

Name: Phillipa Timmins

Organization: Aurox Ltd

Title-Subject: [Filtered] Aurox On-line Conference on Microscopy 7th and 8th April 2020

Message: Dear All,

I hope you are all staying safe in these troubling and turbulent times. We send out our best wishes
from Aurox.

We have had a hugely positive response to our upcoming On-line Conference on Microscopy on 7th and
8th April.
We have a fantastic line up of speakers from academia and industry covering a range of topics
including: Raman Microscopy, Optical Diffraction Tomography, Cryo-microscopy, Super resolution, Live
cell imaging, AI, Image processing, Adaptive Optics, Mesolens, Expansion Microscopy as well as
keynote by Tony Wilson and updates from various companies.

I just wanted to let you know that the deadline to register your interest in attending is this
Friday at 2PM UK time.

It is free to attend and you can find the program and sign up page here:

http://www.aurox.co.uk/aurox-confocal-microscope-conference.php

I look forward to seeing you all there

Best wishes

Phillipa


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From: Xander :      andralbi54nuxdo-at-gmail.com
Date: Sat, 04 Apr 2020 19:19:38 -0800
Subject: re: Social SEO

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Title-Subject: [Filtered] Clinical EM lab

Message: Hello colleagues,
Does anyone know how many clinical EM labs are in America? our department manager would like to
know, and may need help in the future.

Thank you,

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