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From: zaluzec-at-microscopy.com
Date: Fri, 3 Jan 2020 08:22:59 -0600
Subject: [Microscopy] Happy New Year 2020

Contents Retrieved from Microscopy Listserver Archives
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Happy New Year Colleagues;

Welcome to the another year of operation of the Microscopy ListServer
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From: gary_laevsky-at-yahoo.com
Date: Mon, 6 Jan 2020 07:51:12 -0600
Subject: [Microscopy] Big Data, Big Problems; Light-sheet workshop Princeton University,

Contents Retrieved from Microscopy Listserver Archives
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Email: mlynn-at-ameslab.gov Name: Matthew Lynn

Organization: Ames Laboratory, Dept. of Energy

Title-Subject: [Filtered] TEM: Postdoctoral position at the Ames Laboratory

Message: The Division of Materials Science and Engineering (DMSE) at the Ames Laboratory, a
Department of Energy National Laboratory affiliated with Iowa State University, is searching for a
qualified Postdoctoral Research Associate.

This is an opportunity to support a diverse range of research programs using advanced
aberration-corrected STEM and associated techniques.
More details and application instructions can be found here:

https://isu.wd1.myworkdayjobs.com/en-US/IowaStateJobs/job/Ames-IA/Postdoc-Research-Associate_R444



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From fadecice330ojoi-at-gmail.com Mon Jan 6 07:32:29 2020
Return-Path: {fadecice330ojoi-at-gmail.com}
Received: from gmail.com (a3.68474.cn [23.228.73.183] (may be forged))
by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 006DWSaq013173
for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 6 Jan 2020 07:32:28 -0600
Message-ID: {029F40E5.7510914E-at-gmail.com}

Dear Colleagues,


It's the beginning of the new year and it's time to plane your future training opportunities!


We are excited to be offering the first, of what promises to be annual, Big Data, Big Problems light-sheet workshop, held at Princeton University, Princeton, NJ, USA.  The course will run July 27-July 31, 2020.


Applications are due April 15, 2020.  Apply through the course web site at BigDataBigProblems.com.


Multi-dimensional live and fixed microscope data can be collected in so many ways, and the various solutions seem to grow almost daily.  There are many courses that focus on general optical principals and the use of conventional microscope platforms such as widefield fluorescence, confocal and multiphoton imaging, but none that are specifically designed to demystify rapidly evolving and increasingly prescient methods such as light-sheet imaging.  Ultimately this is the goal of this course, what is light-sheet microscopy in all its flavors, what can you do with it, how do you choose between platforms and once you have a system or are proficient with a device what do you do with the data?  This week long course brings together experts in conventional optics, all aspects of light sheet microscopy and image analysis to help you bring your light-sheet based cellular, animal and large sample/cleared sample imaging to the next level.  From the syllabus you will see that we start out with principals and choices and then move into specific systems instructed by both academic and industry faculty.  In fact, we have will have almost all the available commercial solutions on site for you to use.  We will provide samples, but equally you are welcome to use your own.   Our goal is that you will return to your home institution fully capable to implement and use these truly exciting new tools.


Course Speakers;

Amy Elliot National Institutes of Health

Holly Gibbs Texas A&M

Elizabeth Hillman Columbia University

Jan Huisken Morgridge Institute for Research

Gary Laevsky Princeton University

Talley Lambert Harvard University

Wesley Legant University of North Carolina

Paul Maddox University of North Carolina

Alison North The Rockefeller University

Eszter Posfai Princeton University

Doug Richardson Harvard University

Kelly Seagraves Princeton University

Hari Shroff National Institutes of Health

Claudette St. Croix University of Pittsburgh

Sebastian Streichan University of California Santa Barbara

Jared Toettcher Princeton University

Simon Watkins University of Pittsburgh

 
Applications are due April 15, 2020.  Apply through the course website, BigDataBigProblems.com

 
Course Directors

Gary Laevsky, Princeton University

Simon Watkins, University of Pittsburgh



--

Best,


Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Co-Founder, North Atlantic Microscopy Society (NAMS) 
https://namsmicroscopy.com/
Dept. of Molecular Biology
Washington Rd.
Princeton University 
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310


North Atlantic Microscopy Society Spring Meeting at UPENN, April 23, 2020.


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41, 37 -- Date: Mon, 6 Jan 2020 12:48:19 +0000 (UTC)
41, 37 -- From: Gary Laevsky {gary_laevsky-at-yahoo.com}
41, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
41, 37 -- Message-ID: {1153505689.4734631.1578314899793-at-mail.yahoo.com}
41, 37 -- Subject: Big Data, Big Problems; Light-sheet workshop Princeton University,
41, 37 -- July 27-31, 2020
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From: microscopy.listserver-at-gmail.com
Date: Mon, 6 Jan 2020 10:07:49 -0600
Subject: [Microscopy] viaWWW: question about fib milling parameters on polymer membrane

Contents Retrieved from Microscopy Listserver Archives
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Email: helene_roberge-at-hotmail.fr Name: hÈlËne Roberge

Organization: imn

Title-Subject: [Filtered] question about fib milling parameters on polymer membrane

Message: Dear Colleagues,

First at all, Happy New Year !

then, I have a question for you :
i started my phd in last october and i work on polymer membrane. My goal is to image, in a fist
time, the 3D structure of my membrane. For this, i want to use the FIB/SEM available in my lab. I
know this is fragile sample and i need to adapt my parameters for milling my surface without damage
them.

This is my question :
Someone alrady use this technique on polymer membrane ? And have an idea of optimal parameters
(current, energy, deep, dual time..) for use the ion beam ?
I have PES and PAN membranes. I need reference parameters for this type of sample.

Thanks a lot and have a nice day

Best regards

HÈlËne Roberge
Phd student
IMN - Institut des MatÈriaux Jean Rouxel
2, rue de la HoussiniËre - BP 32229
44 322 NANTES CEDEX 3
https://www.cnrs-imn.fr/
0240376316


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From: gary_laevsky-at-yahoo.com
Date: Mon, 6 Jan 2020 12:06:26 -0600
Subject: [Microscopy] North Atlantic Microscopy Society (NAMS) Spring Meeting; April 23,

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

I’m excited to announce that I’ll be hosting the spring 2020 meeting of the North Atlantic Microscopy Society (NAMS) here at the Perelman School of Medicine at the University of Pennsylvania on April 23rd.

NAMS was founded by Gary Laevsky and Paul Shao at Princeton University. Its mission is to promote microscopy education and to provide researchers and vendors in the region with opportunities to connect and hear about the latest applications of microscopy techniques. 

The focus of this meeting will be Correlative Light-Electron Microscopy (CLEM). Light and electron microscopy (EM) speakers will highlight their respective techniques, while CLEM speakers will demonstrate the benefits of bringing the two modalities together.

8:15 - 9:00 am: Registration and coffee
9:00 - 9:20:            Introductory remarks
9:30 - 10:15:           Vera Moiseenkova-Bell (UPenn - Cryo EM)
10:15 - 11:00:          Tim Mosca (Thomas Jefferson University - light microscopy)
11:00 - 11:30:          Break
11:30 - 12:30 pm:       Tech Bites (5-minute lightning talks by vendors)
12:30 - 2:00:           Lunch/Vendors
2:00 - 3:00:            Tatyana Svitkina (UPenn - CLEM)
3:00 - 4:00:            Poster Session/Vendors (snacks & refreshments provided)
4:00 - 5:00:            Justin Taraska (NIH - CLEM)

You can register for the meeting here: https://namsmicroscopy.com/meeting-registration

I hope to see some of you here in April!

Andrea

Andrea L. Stout, Ph. D.
Director,  CDB Microscopy Core Facility
Perelman School of Medicine at the University of Pennsylvania
1107 BRB 2/3
421 Curie Boulevard
Philadelphia, PA 19104

web: http://www.med.upenn.edu/cdbmicroscopycore/
Twitter: -at-CDBMicroCore


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From: microscopy.listserver-at-gmail.com
Date: Mon, 6 Jan 2020 18:57:21 -0600
Subject: [Microscopy] viaWWW:Reminder EGU 2020: Advances in microanalysis: Insights into

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Email: renelle.dubosq-at-gmail.com Name: Renelle Dubosq

Organization: University of Ottawa

Title-Subject: [Filtered] Reminder EGU 2020: Advances in microanalysis: Insights into nanoscale
trace element heterogeneities

Message: Dear colleagues,

We would like to invite submissions of abstracts to our EGU 2020 session applying advanced
microanalysis techniques to investigate chemical heterogeneities.

GMPV1.3 "Advances in microanalysis: Insights into nanoscale trace element heterogeneities"
Convener: Renelle DubosqECS | Co-conveners: Tyler BlumECS, Sandra Piazolo

Invited speakers:
Dr. Desmond Moser, Western University, will present on trace elements and microstructures from Early
Mars and the geochronology of habitability

Dr. Ana Cernok, Royal Ontario Museum, will present on shock‐induced microtextures in lunar
apatite and merrillite

Please follow this link: https://meetingorganizer.copernicus.org/EGU2020/session/35196 for a full
description of the session.

Abstract submission (deadline: 15 January 2020, 13:00 CET)

Looking forward to seeing you in Vienna,
Best regards,

Renelle, Tyler and Sandra

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From: microscopy.listserver-at-gmail.com
Date: Mon, 6 Jan 2020 18:57:51 -0600
Subject: [Microscopy] viaWWW:Biological TEM Workshop, March 11

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Email: johnshields59-at-gmail.com Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] Biological TEM Workshop, March 11

Message: *Biological TEM Workshop*
This intensive, three-day workshop provides a practical and basic theoretical introduction to the
Transmission Electron Microscope and biological sample preparation techniques. Each day will consist
of lecture, discussion and *hands-on* training led by GEM staff.
Who: Anyone requiring training on TEM and biological sample preparation. The workshop will be
limited to 6 participants based on the availability of equipment.
When: Wednesday through Friday, March 11-13 2020, 8am-5pm each day (lunch is provided)
Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602

Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up.
Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login
Deadline: March 4, 2020


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From: microscopy.listserver-at-gmail.com
Date: Mon, 6 Jan 2020 18:58:35 -0600
Subject: [Microscopy] viaWWW: jeol JSM 6360 SEM error codes

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Title-Subject: [Filtered] jeol JSM 6360 SEM

Message: Does anyone have any information on error codes, specifically error code 147 Vacuum system
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From: diller-at-stefan-diller.com
Date: Wed, 8 Jan 2020 05:17:36 -0600
Subject: [Microscopy] Activated Carbon - how to measure active surface

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

one of my customers is producing active carbon, I suppose, from different kind of wood.

Is there any (SEM microscopic) way to find out the active surface value in square meters per gram of carbon? In a more precise way
than to estimate it?


Thanks, and happy new year

Stefan


--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
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12, 28 -- From: stefan diller {diller-at-stefan-diller.com}
12, 28 -- Subject: Activated Carbon - how to measure active surface
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From: wesaia-at-iastate.edu
Date: Wed, 8 Jan 2020 11:05:59 -0600
Subject: [Microscopy] Activated Carbon - how to measure active surface

Contents Retrieved from Microscopy Listserver Archives
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In a word, no.

My understanding is that the pore structure would push the limit of SEM to characterize it. I don't think there would be any reliable way to correlate the microstructure with an estimate of specific surface area. And if the operator or SEM was having an off day, they would fail to resolve the porosity and thus fail to properly compare samples.

I recall that BET is the norm for measuring specific surface. I presume it works for activated carbon. I think it would give much more reliable number than anything that could be done microscopically.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
X-from: diller-at-stefan-diller.com {diller-at-stefan-diller.com}
Sent: Wednesday, January 08, 2020 5:18 AM
To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

Dear All,

one of my customers is producing active carbon, I suppose, from different kind of wood.

Is there any (SEM microscopic) way to find out the active surface value in square meters per gram of carbon? In a more precise way than to estimate it?


Thanks, and happy new year

Stefan


--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


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12, 28 -- Subject: Activated Carbon - how to measure active surface
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From: owenha-at-uwm.edu
Date: Fri, 10 Jan 2020 09:54:25 -0600
Subject: [Microscopy] TEM - Ultramicrotome Light for RMC MT-7000

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

Apologies for the double post, but the deadline for our advertised
3-year postdoc position in STEM imaging at Monash University has been
extended to Friday 24th January. If you know anyone interested who may
not have been able to apply over the holiday period, there's still time.

Full details at:
http://careers.pageuppeople.com/513/cw/en/job/600527/research-fellow-in-atomicscale-structure-determination-in-thick-nanostructures

Kind regards,
Scott


On 22/11/2019 8:08 am, Scott Findlay wrote:
} Dear colleagues,
}
} I'm advertising a 3-year postdoc position in atomic-scale structure
} determination in thick nanostructures at the School of Physics and
} Astronomy, Monash University, Australia.  Further details below.
}
} Please bring this to the attention of anyone who may be interested.
}
} Many thanks,
} Scott Findlay
}
} ___________________
}
} Position Descriptions and application details at:
} http://careers.pageuppeople.com/513/cw/en/job/600527/research-fellow-in-atomicscale-structure-determination-in-thick-nanostructures
}
}
} Position overview: The Research Fellow will work on developing methods
} for atomic-scale structure determination via scanning transmission
} electron microscopy. This project aims to develop a theoretical and
} computational toolkit for structure retrieval at atomic resolution that
} is robust in the presence of multiple scattering (“dynamical
} diffraction”) of the electron probe, and to apply it to large
} experimental data sets obtained from the new generation of fast-readout
} electron detectors. The project may draw on methods from inverse
} scattering theory, phase retrieval, iterative algorithms, machine
} learning, and high-performance computing.
}
} Duration: 3-year fixed-term appointment
} Remuneration package: $68,040 - $92,343 pa Level A (plus 17% employer
} superannuation)
} Closing date: Friday 10th January, 2020

--
*SCOTT FINDLAY*
Senior Lecturer

*Monash University*
School of Physics and Astronomy
G08, New Horizons Centre, 20 Research Way, Clayton Campus
Clayton, VIC 3800
Australia

T: +61 3 9902 4943
E: scott.findlay-at-monash.edu {mailto:scott.findlay-at-monash.edu}
monash.edu {http://monash.edu}

==============================Original Headers==============================
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From normanatlas54ykur-at-gmail.com Wed Jan 8 16:53:37 2020
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Hi Everyone,

We are in the midst of refurbishing an RMC MT-7000 ultramicrotome to replace our MT-7 teaching ultramicrotome that has developed a problem we haven't been able to fix. Unfortunately, the instrument I acquired is missing the horizontal overhead light (but luckily, not the mounting bar). RMC has been fantastic in support for this project, but doesn't have a light available that I can purchase. We've thought about modifying the light from the MT-7, but I still have hopes for getting it going again.

If anyone should happen to have an out of service MT-7000 and would be willing to part with the light fixture, curly cord and/or the plug for the light, please let me know. I have images of the overhead light available that I can send by regular email. The lamp from our other MT-7000 works perfectly with the unit under construction, so no worries about the rest of the circuit.

Thanks for any help,
Heather

Heather A. Owen, Ph.D.
Director, Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
3209 N. Maryland Ave.
Milwaukee, WI† 53211

(414)229-6816

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6, 70 -- From: Heather A Owen {owenha-at-uwm.edu}
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6, 70 -- Subject: TEM - Ultramicrotome Light for RMC MT-7000
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From: Louis.Gambino-at-jmusa.com
Date: Fri, 10 Jan 2020 14:45:41 -0600
Subject: [Microscopy] Job Posting for Scientist II - Electron Microscopy

Contents Retrieved from Microscopy Listserver Archives
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Job Location: Johnson Matthey, Clean Air, Wayne, PA, USA

Job Description: The Development Analytical Services (DAS) Scientist II takes day-to-day responsibility for OM and SEM instrumentation and provides support to R&D projects that generate new products, processes and understanding of commercial value to JM while building an understanding of the science involved. The incumbent will perform routine analytical analyses using the scanning electron microscopy and a significant amount of non-routine work using existing analytical procedures that serve to characterize the properties, function, or composition of catalytic materials or materials of catalytic interest. The incumbent supports JM through all aspects of catalytical material discovery and delivery including analytical analysis, liaise with internal customers, and support of JM processes and practices. Individual will have responsibility for managing projects from inception to close.

For more details and how to apply: {https://chu.tbe.taleo.net/chu01/ats/careers/requisition.jsp?org=JOHNSONMATTHEY&cws=1&rid=9712} .

Louis Gambino, PhD
Associate Scientist
Johnson Matthey
436 Devon Park Drive
Wayne, PA 19087-1816
If the reader of this email is not the intended recipient(s), please be advised that any dissemination, distribution or copying of this information is strictly prohibited. Johnson Matthey Inc. has its main place of business at 435 Devon Park Drive, Wayne, PA, 19087 USA. While Johnson Matthey aims to keep its network free from viruses you should note that we are unable to scan certain emails, particularly if any part is encrypted or password-protected, and accordingly you are strongly advised to check this email and any attachments for viruses. The company shall NOT ACCEPT any liability with regard to computer viruses transferred by way of email. Please note that your communication may be monitored in accordance with Johnson Matthey internal policy documentation.


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From: microscopy.listserver-at-gmail.com
Date: Sat, 11 Jan 2020 08:38:16 -0600
Subject: [Microscopy] viaWWW:GWNIC CLEM WorkshopJune 8-12, 2020

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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner

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Title-Subject: [Filtered] GWNIC CLEM WorkshopJune 8-12, 2020

Message: Greeting Microscopy Listers

The Nanofabrication and Imaging Center at George Washington University will be holding its 4th
annual CLEM workshop June 8-12, 2020 in Washington DC. If you are interested in applications and
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From: microscopy.listserver-at-gmail.com
Date: Sat, 11 Jan 2020 08:43:08 -0600
Subject: [Microscopy] viaWWW:Upcoming Microscopy Workshops at EMS Microscopy Academy

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Title-Subject: [Filtered] Upcoming Microscopy Workshops at EMS Microscopy Academy

Message: Materials Ultramicrotomy
February 24, 2020 For those unfamiliar with microtomy to prepare for the workshop.
February 25 - 27, 2020
This workshop will introduce individuals to the unique application of ultramicrotomy to materials.
Learn more at https://www.emsmicroscopyacademy.com/product-page/materials-feb20
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From: diller-at-stefan-diller.com
Date: Sun, 12 Jan 2020 14:09:44 -0600
Subject: [Microscopy] Re: Activated Carbon - how to measure active surface -

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

thanks for the replies to this topic. I suppose my customer will do it the chemical way...

But it had been nice to experience some insights in this peculiar topic ;-)


Stefan



}
}
}
}
}
} -----Original Message-----
} From: diller-at-stefan-diller.com {diller-at-stefan-diller.com}
} Sent: Thursday, 9 January 2020 12:24 AM
} To: Harland, Duane {Duane.Harland-at-agresearch.co.nz}
} Subject: [Microscopy] Activated Carbon - how to measure active surface
}
}
}
}
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} Dear All,
}
} one of my customers is producing active carbon, I suppose, from different kind of wood.
}
} Is there any (SEM microscopic) way to find out the active surface value in square meters per gram of carbon? In a more precise way than to estimate it?
}
}
} Thanks, and happy new year
}
} Stefan
}
}
} --
}
}
} -----------------------------------------------------
} Stefan Diller - Scientific Photography
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From: microscopy.listserver-at-gmail.com
Date: Mon, 13 Jan 2020 21:36:58 -0600
Subject: [Microscopy] viaWWW:position available - Applications Specialist in FIB-SEM Tescan

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello and happy New Year to all,

The Analytical Instrumentation Facility (AIF) and Research Triangle
Nanotechnology Network (RTNN) at North Carolina State University, in
partnership with Protochips and ThermoFisher Scientific, are pleased
to announce the first annual In Situ Microscopy Congress (ISMC). The
meeting will be held at North Carolina State University in Raleigh, NC
on March 2nd and 3rd, 2020.

This is a two day workshop which provides one the opportunity to learn
about liquid phase and gaseous phase in situ capabilities, and
includes two plenary talks from leaders in the respective fields along
with hands-on demonstrations of liquid and gas cell holders and our
Talos F200X, probe-corrected Titan, and Quanta 3D FIB/SEM platforms.

This workshop is open to all. Attendees are encouraged to submit an
abstract to be considered for an oral or poster presentation. To
register, or for more information, please visit the following
registration link:

https://www.eventbrite.com/e/in-situ-microscopy-congress-tickets-83202188987

We look forward to seeing you,
Chris
--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
https://www.aif.ncsu.edu/
Cell: 267-496-0587

==============================Original Headers==============================
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Email: tescanmicroscopy-at-gmail.com Name: dj miller

Organization: Tescan USA

Title-Subject: [Filtered] position available - Applications Specialist in FIB-SEM Tescan USA

Message: TESCAN USA, a fast-growing solution provider in electron, ion and x-ray CT in the
microscope business, is looking for an Applications Specialist in FIB-SEM. This position is
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Microscopy and intimate familiarity with FIB-SEM tools and techniques such as TEM sample
Preparation, 3D cross-sectioning and reconstruction (image, EDS & EBSD), delayering, nano-probing,
circuit edit, lithography and other. Experience in other techniques such as TOF-SIMS, cryo sample
preparation, APT, TEM would be a plus. Excellent communication in English is required. This
position located in the Pleasanton, CA, but will require travel up to 50% of the time.

TESCAN USA will only employ individuals who are legally authorized to work in the United States for
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the successful completion of a background investigation and drug screening.
TESCAN USA offers competitive salaries and benefits.
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From: microscopy.listserver-at-gmail.com
Date: Mon, 13 Jan 2020 21:37:40 -0600
Subject: [Microscopy] viaWWW:Staff position CLEM and Bio EM at Monash University,

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Email: georg.ramm-at-monash.edu Name: Georg Ramm

Organization: Monash Ramaciotti Centre
Title-Subject: [Filtered] Staff position CLEM and Bio EM at Monash University, Melbourne, Australia

Message: We are looking for a staff member with expertise in CLEM to join our team at the Monash
Ramaciotti Centre at Monash University (https://www.monash.edu/researchinfrastructure/cryo-em)
Senior Research Officer - Correlative Light and Electron Microscopy
Location: Clayton campus, Melbourne, Australia

Employment Type: Full-time, 3 year Fixed-term appointment

Remuneration: $98,155 - $108,345 pa HEW Level 08 (plus 17% employer superannuation)

Contact: Georg Ramm, Georg.ramm-at-monash.edu, +61-3 - 9905 1280

Closing date: 31 January 2020
Link to job advertisement:
https://careers.pageuppeople.com/513/cw/en/job/599948/senior-research-officer-correlative-light-and-electron-microscopy

As the successful candidate you will:

Manage, plan, coordinate and oversee EM projects in collaboration with users Develop new protocols
for correlative light and electron microscopy and life sciences EM techniques and apply them to
research projects
Teach specialised EM techniques such as CLEM and immuno EM to Monash researchers and to the wider
Australian and international EM community
Perform microscopy and related EM techniques including immuno EM, (cryo-) ultramicrotomy, high
pressure freezing, and cryo-preparation

The Monash Ramaciotti Centre is a leading facility for life sciences electron microscopy. It houses
Australiaís first Titan Krios microscope, a cryo-FIBSEM Helios G4 with Leica VCT500 cryo-stage, a
Talos Arctica, as well as two 120keV TEMs and a FESEM. A suite of advanced sample preparation and
other equipment is available, including a Zeiss LSM900 Airyscan with Linkam cryo-stage, a Wohlwend
high pressure freezer, Leica AFS2 and FC7 cryo-ultramicroscopes. The facilityís expert team supports
and collaborates on a large number of bio EM techniques ranging from standard SEM and TEM to immuno
EM, correlative light and electron microscopy, cryotomography and single particle analysis.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 15 Jan 2020 07:46:58 -0600
Subject: [Microscopy] viaWWW:Light Leakage from Microscope into Digital Camera

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Email: bowmanpond-at-gmail.com Name: Mike Codner

Title-Subject: [Filtered] Light Leakage from Microscope into Digital Camera

Message: While familiarizing myself with a newly purchased Swiftcam 18 MP Microscope Camera, I was
having a problem mating it to my LW Scientific Revelation III Binocular Microscope using Swift's 3.0
software and Windows 10. Upon viewing pond water specimens, the background lighting kept changing
from white to darker colors and vice-versa. I had made all the adjustments the manual had
recommended including exposure, white balance, color settings, and the like. Nothing worked. To
make a long story short, the problem resolved itself when I plugged the non-camera binocular with a
black rubber stopper after removing the lens. Apparently there had been light leakage from this
binocular into the one containing the camera. Just wanted to alert your members since this problem
was very frustrating and I could find no helpful information either in the manual or online.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 15 Jan 2020 12:52:38 -0600
Subject: [Microscopy] viaWWW Post Doctoral Position - Developing a Cryo-compatible Specimen

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Email: sichen-at-anl.gov

Name: Si Chen

Organization: Argonne National Laboratory

Title-Subject: [Filtered] Post Doctoral Position - Developing a Cryo-compatible Specimen Prep
workflow for Xray and Electron Microscopy -at- Argonne National Laboratory

Message: Position Description

The X-ray Science Division (XSD) of Advanced Photon Source (APS) at Argonne National Laboratory is
looking for a Postdoctoral Researcher who will be responsible for developing novel apparatus and
methods to enable multi-scale and multi-modality analysis of materials via both electron microscopy
and X-ray microscopy at the synchrotron facility. The research aims to deliver a cryo-compatible
workflow and supporting techniques to optimize sample preparation for X-ray fluorescence nanoprobe
as well as the combined use of multiple analytical platforms. It will also involve software
development for image registration across different analytical platforms and correlative data
analysis. The successful candidate will work in a multidisciplinary team environment including
physicists, chemists, biologists and computer scientist. The candidate will lead the effort in
developing the methodology, the deployment at the APS beamlines, as well as applications to
materials, biological and environmental sciences. Results will be reported in appropriate forms:
publications in refereed journals and oral presentations at meetings, conferences, and seminars. The
position will begin in April 2020. It is a one-year position, and renewable for a second year.

Position Requirements

*Ph.D. degree in physics, materials science, engineering, or a related discipline;
*Comprehensive knowledge in at least two of the following fields: X-ray physics, X-ray microscopy,
electron microscopy, image processing;
*Considerable experience with electron microscopy, focused ion beam, synchrotron facility,
preferably cryogenic instruments;
*Strong data analysis and trouble-shooting skills;
*Considerable experience with programming and software tools such as python and git;
*Experience with automation, control, and instrument infrastructure is a plus;
*Knowledge of biology, chemistry is a plus;
*Experience with cryogenic is a plus;
*Demonstrated experience working successfully as part of a team in a collaborative and
multidisciplinary scientific environment;
*Ability to communicate effectively with internal and external collaborators and ability to work in
a team environment.

Additional Details and Application Forms can be found at this URL

https://careers.peopleclick.com/careerscp/client_argonnelab/post_doc/jobDetails.do?functionName=getJobDetail&jobPostId=8696&localeCode=en-us

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14, 54 -- Subject: viaWWW Post Doctoral Position - Developing a Cryo-compatible Specimen
14, 54 -- Prep workflow for Xray and Electron Microscopy -at- Argonne National Laboratory
14, 54 -- References: {202001151822.00FIMoP5026403-at-microscopy.com}
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From: microscopy.listserver-at-gmail.com
Date: Thu, 16 Jan 2020 19:16:39 -0600
Subject: [Microscopy] viaWWW: Syllabus/Lectures for Cellular & Molecular Electron

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Email: whiteto-at-missouri.edu Name: Tommi White

Organization: University of Missouri Electron Microscopy Core

Title-Subject: [Filtered] Cellular & Molecular Electron Microscopy

Message: Hello Microscopy Listserve,

I am taking over a graduate level class entitled "Cellular & Molecular Electron Microscopy" from a
retiring faculty member. Would any of you be so kind to share syllabus/lecture materials that I
could use in this course? Of course, full credit will be given to the provider of the information!
:) Thanks in advance to helping educate the next generation of electron microscopists and light
that fire.
Tommi
Tommi A. White, Ph.D.
Director, Electron Microscopy Core
Assistant Research Professor, Biochemistry
University of Missouri
Mail: W117 Veterinary Medicine Bldg
1520 E Rollins St., Columbia, MO 65211
EMC: 573-882-8304
Direct: 573-884-7338
Email: whiteto-at-missouri.edu
Web: http://emc.missouri.edu
Tweets: -at-MizzouEMC


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From: wsalmon-at-wi.mit.edu
Date: Tue, 21 Jan 2020 15:32:39 -0600
Subject: [Microscopy] 2020 Analytical and Quantitative Light Microscopy course

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Colleagues,

There is still plenty of time to apply for Analytical and Quantitative
Light Microscopy 2020, held at the Marine Biological Laboratory, Woods
Hole, MA,
USA. The course will run April 28-May 8, 2020.

Applications are due February 5, 2020. Apply through the course web site at
http://www.mbl.edu/aqlm. We especially invite those from underrepresented
groups to apply. Reach out if you have any questions!

AQLM is a comprehensive and intensive course in light microscopy for
researchers in biology, medicine, and material sciences. This course
provides a systematic and in-depth examination of the theory of image
formation and application of digital methods for exploring subtle
interactions between light and the specimen. This course emphasizes the
quantitative issues that are critical to the proper interpretation of
images obtained with modern wide-field microscopes, confocal microscopes,
and new emerging technologies.

Applications are due February 5, 2020. Apply through the course web site at
http://www.mbl.edu/aqlm.

Some refer to it as "microscopy boot camp" because we work hard, but we
have a lot of fun in the process!

Course Directors:
Peter Kner, University of Georgia
Paul Maddox, University of North Carolina at Chapel Hill
Wendy Salmon, Whitehead Institute for Biomedical Research
Course Laboratory Director:
Gary Laevsky, Princeton University

Happy Imaging!
Peter, Paul, Wendy and Gary


Best,
Wendy

--------------------
Wendy C Salmon, M.A.
Light Microscopy Specialist
W.M. Keck Facility for Biological Imaging
Whitehead Institute for Biomedical Research
455 Main St., Rm 447
Cambridge, MA 02142
e: wsalmon-at-wi.mit.edu
w: microscopy.wi.mit.edu

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From: korinek-at-mcmaster.ca
Date: Tue, 21 Jan 2020 16:06:23 -0600
Subject: [Microscopy] CCEM TEM Summer School 2020

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The CCEM just announced that it will be holding its annual summer school on electron microscopy from June 8 - 12, 2020!

A 5-DAY COURSE for users with experience in electron microscopy, on the fundamentals of aberration-corrected imaging, electron energy loss spectroscopy, energy dispersive X-ray spectroscopy, electron tomography, ultimate physical limits (beam damage and resolution), DPC microscopy and the use of aberration-corrected electron microscopes. The aim is to provide students a device in solving characterization problems with the help of experts. The course will include lectures given by experts in the use of the technique and experts in electron optics, alignment and optimization of electron microscopes and EELS spectrometers. Students will have plenty of opportunities for hands-on training on the alignment and operation of the electron microscopes with the experts from the microscope and spectrometer companies. Two FEI Titan microscopes with correctors and monochromators (GIF Quantum and K2) will be used for training, as well as a Talos F200X. Several hands-on data processing sessions are also organised.

DATE: June 8 - 12, 2020

COST: All meals and course notes are included in the registration fee ranging from $700.CDN/full-time students to $2000.CDN/industry researchers. Accommodation will be separate and the responsibility of attendees (see full details on registration form).

REGISTRATION: Register online by January 31, 2020:
https://ccem.mcmaster.ca/ccem-summer-school-2020

--†
Dr. Andreas Korinek

Manager

Canadian Centre for Electron Microscopy
McMaster University
1280 Main Street West, Hamilton ON Canada, L8S 4M1
phone: +1 905-525-9140 ext 20400



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From: microscopy.listserver-at-gmail.com
Date: Wed, 22 Jan 2020 08:40:58 -0600
Subject: [Microscopy] viaWWW:Postdoc/Research Associate Position Available - McMaster

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Email: bassimn-at-mcmaster.ca

Name: Nabil Bassim

Organization: McMaster University

Title-Subject: [Filtered] Postdoc/Research Associate Position Available - McMaster University

Message: The Bassim research group is seeking a postdoc or research associate who will work on a
varied portfolio of projects, including developing novel 2-D van der Waals heterostructures,
catalysts, and metamaterials.
The chosen candidate would also be encouraged to develop their own microscopy techniques using the
CCEM infrastructure. The research projects involve strong collaboration with synthesis teams, and
proven ability to work in interdisciplinary groups should be demonstrated. Teaching, communication,
and presentation skills are part of a successful post-doctoral or research associate position, and
academic/industrial/governmental experience in these areas should be clearly detailed. Day-to-day
supervisory tasks with graduate students in the CCEM and within the Bassim research group would be
expected, and examples of administrative, project management, and supervisory skills would be a plus.
Requirements for the role include:
MANDATORY
- A Ph.D. in Materials Science or Physics
- Experience in aberration-corrected imaging and electron energy loss spectroscopy
- A strong track record in publishing TEM-related research

DESIRED
- Interest in mentoring graduate students
- Experience with in-situ techniques
- Capability to perform image simulations and scattering theory
- Sample preparation capabilities using FIB
Pay will range based on level of experience and track record. The appointment is for 1 year,
renewable up to 3 years.

Located at McMaster University in Hamilton, Ontario, the work would be performed at the Canadian
Centre for Electron Microscopy (CCEM), which is home to 10 technical staff and hundreds of users
with many diverse interests. The CCEM has a very elaborate suite of advanced instrumentation with
plans for future expansion. Hamilton is a lovely city in Southern Ontario, located midway between
Toronto and Niagara Falls with a mild (by Canadian standards) winter.

If you are interested, please contact bassimn at mcmaster.ca with a CV and several references.


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From: microscopy.listserver-at-gmail.com
Date: Fri, 24 Jan 2020 18:09:33 -0600
Subject: [Microscopy] viaWWW: FEI (Philips) XL30 Upgrade

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Email: wim5-at-lehigh.edu

Name: Bill Mushock

Organization: Lehigh Unbiversity

Title-Subject: [Filtered] FEI (Philips) XL30 Upgrade

Message: We are looking to extend the life of our WinNT XL30 ESEM with an upgrade. It seems FEI no
longer offers an upgrade but there are apparently several options available through third party vendors.

I would appreciate hearing directly from anyone willing to share their experiences who have gone the
third party route.

Also if you're contemplating decommissioning a Win 2000 XL30 in the near future and are looking for
someone to take it off your hands, please contact me.

Thanks,
Bill Mushock
wim5-at-lehigh.edu

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From: diller-at-stefan-diller.com
Date: Sat, 25 Jan 2020 06:21:46 -0600
Subject: [Microscopy] Re: viaWWW: FEI (Philips) XL30 Upgrade

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Hello Bill,

here in Germany there is a manufacturer who substitutes the old electronics of the XL30 (even ESEM) with a completely new one.

Refurbishing can also be done at your site and takes some days.

Please check at

https://www.pointelectronic.de/en/products/sem-control/

...Just a satisfied customer...


Best wishes,

Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 25.01.20 um 01:17 schrieb microscopy.listserver-at-gmail.com:
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} Email: wim5-at-lehigh.edu
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} Name: Bill Mushock
}
} Organization: Lehigh Unbiversity
}
} Title-Subject: [Filtered] FEI (Philips) XL30 Upgrade
}
} Message: We are looking to extend the life of our WinNT XL30 ESEM with an upgrade. It seems FEI no
} longer offers an upgrade but there are apparently several options available through third party vendors.
}
} I would appreciate hearing directly from anyone willing to share their experiences who have gone the
} third party route.
}
} Also if you're contemplating decommissioning a Win 2000 XL30 in the near future and are looking for
} someone to take it off your hands, please contact me.
}
} Thanks,
} Bill Mushock
} wim5-at-lehigh.edu
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==============================Original Headers==============================
12, 28 -- From diller-at-stefan-diller.com Sat Jan 25 06:21:46 2020
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12, 28 -- Subject: Re: [Microscopy] viaWWW: FEI (Philips) XL30 Upgrade
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From: microscopy.listserver-at-gmail.com
Date: Sun, 26 Jan 2020 16:40:02 -0600
Subject: [Microscopy] viaWWW:UCT controller

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Email: xbren-at-uw.edu Name: Shirley

Organization: UW

Title-Subject: [Filtered] UCT controller
Message: Hi,
Can someone give me any ideas? One of my lab's ultra-cut microtome(Leica UCT) controller doesn't
work any more, and we found that the power unit is down. We reached Leica, but they said they don't
have this model and don't do repair on this model. The microtome is good, but it's only the
controller that doesn't work. Do you know where to repair/replace this kind of controller?

Many thanks!



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From: zaluzec-at-microscopy.com
Date: Tue, 28 Jan 2020 07:49:11 -0600
Subject: [Microscopy] viaWWW: EELS & EFTEM Analysis Training School April 2020

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Email: jhyun-at-gatan.com Name: John Hyun

Organization: Gatan, Inc.

Title-Subject: [Filtered] EELS & EFTEM Analysis Training School April 2020

Message: EELS & EFTEM Analysis Training School April 2020
April 21 ñ 24, 2020
Gatan R&D Headquarters, Pleasanton, CA

We invite you to our upcoming course to learn best practices to set up your EELS hardware, optimize
experimental protocols, then capture, and extract the maximum amount of compositional and chemical
information from your TEM samples. Topics include:

ï Fundamentals of EELS and energy-filtered imaging in TEM
ï Principles of operation of EFTEM and EELS systems
ï Optimization of EFTEM and EELS data acquisition
ï Quantification of elemental composition
ï Other information provided by EFTEM/EELS and how best to extract it
ï Use of EELS signals to form maps of elemental and chemical composition
ï EFTEM and STEM EELS spectrum imaging techniques
ï Identification of material phases via EELS fine structure mapping
ï Applications to biological and physical science specimens

Register today as seats are limited.

Registration: https://www.gatan.com/company/events/eels-eftem-analysis-training-school-april-2020


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From: microscopy.listserver-at-gmail.com
Date: Wed, 29 Jan 2020 09:13:05 -0600
Subject: [Microscopy] viaWWW: pathological report for mouse kidney EM

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Email: amaliapasolli-at-gmail.comm Name: Amalia Pasolli

Organization: The Rockefeller University

Title-Subject: [Filtered] pathological report for mouse kidney EM

Message: Hi,
Do you know any lab that could do TEM of mouse kidneys, including a pathology report?

Closer to NYC area would be a plus.

Thank you!

AMALIA
Hilda Amalia Pasolli, Ph.D.
Director and Research Associate Professor
Electron Microscopy Resource Center
RRB 120F
The Rockefeller University
1230 York Avenue,
Box 230
New York, NY 10065
Phone 212 327 8325

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From: microscopy.listserver-at-gmail.com
Date: Thu, 30 Jan 2020 09:08:59 -0600
Subject: [Microscopy] viaWWW:Job Vacancy - Optical Microscopist

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Email: julie.blasioli-at-petermac.org Name: Julie Blasioli

Organization: Peter MacCallum Cancer Centre

Title-Subject: [Filtered] Job Vacancy - Optical Microscopist

Message: Location: Peter MacCallum Cancer Centre, Melbourne Australia

Peter MacCallum Cancer Centre is seeking an experienced and highly motivated scientist to join the
Centre for Advanced Histology and Microscopy (CAHM). CAHM encompasses research histology, optical
and electron microscopy and provides researchers with advice, tuition and technical expertise.

The applicant will have a passion for optical microscopy and in-depth understanding of the operation
of optical microscopes, including confocal, super resolution, and multiphoton microscopy. The
applicant will enjoy interacting with researchers and assisting them with their imaging needs.

The position is initially for a fixed term of 12 months with the opportunity for future extension.

Contact Person: Sarah Ellis
Contact Number: +613 8559 7822
Contact Email: sarah.ellis-at-petermac.org
Closing Date: 08 April 2020

To see the position description and apply go to
https://petermac.mercury.com.au/ViewPosition.aspx?id=heS2osrVG/U=&jbc=ere




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From: microscopy.listserver-at-gmail.com
Date: Thu, 30 Jan 2020 09:30:07 -0600
Subject: [Microscopy] Fwd: viaWWW: pathological report for mouse kidney EM

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X-from: Lee Cohen-Gould {lcgould-at-med.cornell.edu}



Hi Amalia-
Happy New Year.
What kind of pathology do you expect?† In the cortex or medulla?† Glomeruli, tubules or calex?
I can send you a protocol for mouse kidney, or we can do it here, but we don't do the pathology
analysis.† You should speak to the people in your animal facility. They should be able to either do
it or refer you to someone, perhaps at the Animal Medical Center.
I could put you in touch with our clinical EM facility here. They specialize in human kidney pathology.
Let me know what you need.
Best,
Lee

*Leona Cohen-Gould, MS, CEMT*

Senior Staff Associate

Co-Director CLC Microscopy & Image Analysis Core

*Weill Cornell Medicine*

Department of Biochemistry

1300 York Ave, A-105

New York, NY 10065

T 212.746.6146

F 212.746.8175

*lcgould-at-med.cornell.edu {mailto:lwschafe-at-med.cornell.edu} *
*
*
*

/If you have a publication or grant (submitted or funded) that utilized data from our Core, please
tell us here: /

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*Sent:* Wednesday, January 29, 2020 10:14 AM
*To:* Lee Cohen-Gould {lcgould-at-med.cornell.edu}
*Subject:* [EXTERNAL] [Microscopy] viaWWW: pathological report for mouse kidney EM



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Email: amaliapasolli-at-gmail.comm Name: Amalia Pasolli

Organization: The Rockefeller University

Title-Subject: [Filtered] pathological report for mouse kidney EM

Message: Hi,
Do you know any lab that could do TEM of mouse kidneys, including a pathology report?

Closer to NYC area would be a plus.

Thank you!

AMALIA
Hilda Amalia Pasolli, Ph.D.
Director and Research Associate Professor
Electron Microscopy Resource Center
RRB 120F
The Rockefeller University
1230 York Avenue,
Box 230
New York, NY 10065
Phone 212 327 8325

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From: microscopy.listserver-at-gmail.com
Date: Sat, 1 Feb 2020 20:32:23 -0600
Subject: [Microscopy] viaWWW: Help Needed - Error Code for SU1510

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Title-Subject: [Filtered] Error Code for SU1510

Message: We purchased a Hitachi SU1510 SEM about 4 years ago and have had some good luck with it but
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Date: Tue, 4 Feb 2020 20:28:12 -0600
Subject: [Microscopy] viaWWW:&M 2020 - Important Submission Info!

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From: microscopy.listserver-at-gmail.com
Date: Wed, 5 Feb 2020 08:45:18 -0600
Subject: [Microscopy] viaWWW:European Microscopy Congress 2020 - Abstract Submission

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Title-Subject: [Filtered] European Microscopy Congress 2020 - Abstract Submission Deadline 1 March 2020

Message: The European Microscopy Congress 2020 (emc2020) in Copenhagen is being hosted by SCANDEM,
and organised by the Royal Microscopical Society under the auspices of the EMS and IFSM. The
European Microscopy Congress is known for being the largest European stage for cross-disciplinary
research. The broad scientific programme will welcome contributions from all over the world,
showcasing the latest research in life sciences, physical sciences and engineering across all
microscopy and imaging techniques.
Over 30 conference sessions, an exhibition with more than 100 companies represented and a brilliant
selection of features such as pre-event workshops and social networking events.



Oral and Poster abstract submission is currently open for the European Microscopy Congress 2020. Go
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The deadline for submitting your abstract is 1 MARCH 2020.

We are accepting abstracts for the following symposia: ï Life Sciences: Applications
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From: koeck-at-kth.se
Date: Thu, 6 Feb 2020 04:24:56 -0600
Subject: [Microscopy] electron source

Contents Retrieved from Microscopy Listserver Archives
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‚Äã

I'm looking for an electron source, used or new, that can produce a beam with the following characteristics:

energy: 1 keV or less
current: in the order of a few microAmps
beam waist diameter: 1 micrometer or less

I'm thinking of a gun and a single condenser lens if that is possible. Small is good.

Something used in a SEM, in e-beam lithography or electron evaporation might be a possibility.

Can anyone recommend something?

All the best,

Philip


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From: vray-at-partbeamsystech.com
Date: Thu, 6 Feb 2020 08:47:32 -0600
Subject: [Microscopy] Re: electron source

Contents Retrieved from Microscopy Listserver Archives
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Hi Keith,

Electron source with beam current of a few uA wouldn't exactly be from
SEM or EBL domain, but.... I would suggest to try speaking to: Kimball
Physics https://www.kimballphysics.com/

No connection other then a satisfied customer here.

Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 2/6/2020 5:25 AM, koeck-at-kth.se wrote:
} ----------------------------------------------------------------------------
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}
} I'm looking for an electron source, used or new, that can produce a beam with the following characteristics:
}
} energy: 1 keV or less
} current: in the order of a few microAmps
} beam waist diameter: 1 micrometer or less
}
} I'm thinking of a gun and a single condenser lens if that is possible. Small is good.
}
} Something used in a SEM, in e-beam lithography or electron evaporation might be a possibility.
}
} Can anyone recommend something?
}
} All the best,
}
} Philip
}
}
} ==============================Original Headers==============================
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From: vray-at-partbeamsystech.com
Date: Thu, 6 Feb 2020 08:47:31 -0600
Subject: [Microscopy] Re: electron source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Keith,

Electron source with beam current of a few uA wouldn't exactly be from
SEM or EBL domain, but.... I would suggest to try speaking to: Kimball
Physics https://www.kimballphysics.com/

No connection other then a satisfied customer here.

Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 2/6/2020 5:25 AM, koeck-at-kth.se wrote:
} ----------------------------------------------------------------------------
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} I'm looking for an electron source, used or new, that can produce a beam with the following characteristics:
}
} energy: 1 keV or less
} current: in the order of a few microAmps
} beam waist diameter: 1 micrometer or less
}
} I'm thinking of a gun and a single condenser lens if that is possible. Small is good.
}
} Something used in a SEM, in e-beam lithography or electron evaporation might be a possibility.
}
} Can anyone recommend something?
}
} All the best,
}
} Philip
}
}
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From: koeck-at-kth.se
Date: Fri, 7 Feb 2020 07:44:50 -0600
Subject: [Microscopy] SV: electron source

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

An update to my question:

Does anybody have a microbeam instrument that's not needed anymore.
I'm interested in the column-part of it.

I need to produce an electron beam with the following characteristics:

energy: 1 keV or less
current: in the order of a few microAmps, up to 10 if possible.
beam waist diameter: 1 micrometer or less

The beam should be convergent with the waist at a distance of about 15 cm from the device.

All the best,

Philip

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From: vray-at-partbeamsystech.com
Date: Fri, 7 Feb 2020 13:30:54 -0600
Subject: [Microscopy] Re: Ask-a-Microscopist: FIB sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

***********************************************************************************
Forwarded from "Ask a Microscopist"
Please remember that the person asking the question is likely not a member the listserver, and
**any reply should go directly to the poster** as well as to the list.
Using the "reply" function in your email does *not* send your answer to the person asking the question.
Please copy their email address from their question.
***********************************************************************************
Name: Aubrey Penn
Email: anpenn-at-ncsu.edu

Avoiding surface damage during FIB sample preparation is fairly
straight-forward: prior to ever exposing sample ion beam it should be
coated with sufficient thickness (~100nm optimal, but } 30nm is a minimal
requirement) of some protective layer that will "absorb" ion beam damage.

Following coatings may typically be used for such protective layer,
depending on nature of the sample: (a) e-beam deposition of C, Pt, Mo,
W, SiOx, etc...; (b) evaporated carbon or metal; (c) sputter coating by
Au, Au/Pd, TiO, Cr, Ir, etc...; (d) conductive polymer coating by
spinning or ultrasonic nozzle dispensing; (e) ink coating (i.e. "Sharpie
trick), etc...

For atomic resolution TEM amorphous layer on the sides of the lamella
would also need to be cleaned, but that is already another question.

Happy sample prepping :)
Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 2/7/2020 11:52 AM, oshel1pe-at-cmich.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} ***********************************************************************************
} Forwarded from "Ask a Microscopist"
} Please remember that the person asking the question is likely not a member the listserver, and
} **any reply should go directly to the poster** as well as to the list.
} Using the "reply" function in your email does *not* send your answer to the person asking the question.
} Please copy their email address from their question.
} ***********************************************************************************
} Name: Aubrey Penn
} Email: anpenn-at-ncsu.edu
} Subject: FIB sample preparation
} Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging?
}
} I'm not a FIB expert, but there are plenty on the microscopy list. There will probably be other people who would like to know the answer to this question, too, so please reply both directly to Penn and to the list.
}
} -------------
} Philip Oshel
} Microscopy Society of America
} Ask a Microscopist
} www(dot)microscopy(dot)org/resources/ask(dot)cfm
}
}
}
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From: vray-at-partbeamsystech.com
Date: Fri, 7 Feb 2020 13:30:54 -0600
Subject: [Microscopy] Re: Ask-a-Microscopist: FIB sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Avoiding surface damage during FIB sample preparation is fairly
straight-forward: prior to ever exposing sample ion beam it should be
coated with sufficient thickness (~100nm optimal, but } 30nm is a minimal
requirement) of some protective layer that will "absorb" ion beam damage.

Following coatings may typically be used for such protective layer,
depending on nature of the sample: (a) e-beam deposition of C, Pt, Mo,
W, SiOx, etc...; (b) evaporated carbon or metal; (c) sputter coating by
Au, Au/Pd, TiO, Cr, Ir, etc...; (d) conductive polymer coating by
spinning or ultrasonic nozzle dispensing; (e) ink coating (i.e. "Sharpie
trick), etc...

For atomic resolution TEM amorphous layer on the sides of the lamella
would also need to be cleaned, but that is already another question.

Happy sample prepping :)
Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 2/7/2020 11:52 AM, oshel1pe-at-cmich.edu wrote:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} ***********************************************************************************
} Forwarded from "Ask a Microscopist"
} Please remember that the person asking the question is likely not a member the listserver, and
} **any reply should go directly to the poster** as well as to the list.
} Using the "reply" function in your email does *not* send your answer to the person asking the question.
} Please copy their email address from their question.
} ***********************************************************************************
} Name: Aubrey Penn
} Email: anpenn-at-ncsu.edu
} Subject: FIB sample preparation
} Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging?
}
} I'm not a FIB expert, but there are plenty on the microscopy list. There will probably be other people who would like to know the answer to this question, too, so please reply both directly to Penn and to the list.
}
} -------------
} Philip Oshel
} Microscopy Society of America
} Ask a Microscopist
} www(dot)microscopy(dot)org/resources/ask(dot)cfm
}
}
}
} ==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Sat, 8 Feb 2020 09:15:44 -0600
Subject: [Microscopy] viaWWW: Invitation to Lehigh Microscopy School 2020

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Email: engineering-at-lehigh.edu

Name: Drs. Kiely and Watanabe, Lehigh University

Organization: Lehigh University

Title-Subject: [Filtered] Invitation to Lehigh Microscopy School 2020

Message: It is our great pleasure to invite you to the 50th annual Lehigh Microscopy School (LMS),
set for May 31-June 5, 2020, here in the Whitaker Laboratory at Lehigh University in Bethlehem, PA.

The school's 2020 offering marks a half century since renowned Lehigh University Professor Joe
Goldstein founded the annual weeklong program to keep colleagues and professionals abreast of
developments in scanning electron microscopy. Since then it has grown to become the largest school
of its kind.
Now in its 50th year, the weeklong school offers basic and advanced courses that combine theory with
lab practice. LMS is proud to partner with some of the top microscopy manufacturers in the world
supplement the nine scanning and transmission electron microscopes in Lehigh's Materials
Characterization Facility, one of the best equipped of its kind in the United States.

Please visit lehigh.edu/microscopy to learn more about LMS and its curriculum; please don't hesitate
to reach out if you have any questions.

We thank you for your interest and look forward to seeing you at LMS 2020!

Kind regards,

Dr. Christopher J. Kiely
Harold B. Chambers Senior Professor of Materials Science and Engineering
Lehigh University
LMS Director

Dr. Masashi Watanabe
Associate Professor of Materials Science and Engineering
Lehigh University
LMS Co-director



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From: microscopy.listserver-at-gmail.com
Date: Sat, 8 Feb 2020 09:16:36 -0600
Subject: [Microscopy] =?UTF-8?Q?viaWWW=3aSession_13d_=e2=80=9cAirborne_particles_and_fibe?=

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Email: elena.belluso-at-unito.it

Name: Elena Belluso

Organization: University of Torino - I

Title-Subject: [Filtered] session 13d ìAirborne particles and fibers: characteristics, sources,
toxicology, and impacts on ecosystems and human healthî, Goldschmidt 2020

Message: Dear Colleague,
We are pleased to invite you to submit an abstract for an oral presentation (or a poster) to the
session 13d: AIRBORNE PARTICLES AND FIBERS: CHARACTERISTICS, SOURCES, TOXICOLOGY, AND IMPACTS ON
ECOSYSTEMS AND HUMAN HEALTHî (Theme 13 https://goldschmidt.info/2020/program/programViewThemes) at
Goldschmidt 2020, the international conference on geochemistry and related subjects, scheduled from
21 to 26 June 2020 in Honolulu, Hawaii.

The key-note speaker of the session is Reto GierÈ (University of Pennsylvania): ìMineralogy,
Chemistry, and Toxicology of Particulate Emissions from Combustion Processesî.

Convenors: Elena Belluso (Universit‡ degli Studi di Torino, Torino, Italy), Janice Brahney (Utah
State University, Logan, UT, USA), Francesco Di Benedetto (Universit‡ degli Studi di Firenze,
Firenze, Italy), Peggy O'Day (University of California, Merced, CA, USA)

Note that the abstract deadline is February, 14, 2020 (https://goldschmidt.info/2020/abstracts)

We look forward to seeing you in Honolulu in June!


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From: microscopy.listserver-at-gmail.com
Date: Sat, 8 Feb 2020 09:17:25 -0600
Subject: [Microscopy] Fwd: Re: viaWWW:TEM Film Issue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Richard E. Edelmann {Edelmare-at-miamioh.edu}

Andrew,

There are really only three factors:

(1) The film. Is it old or bad? Has something changed in the development? (temp or developer aged
etc.)

(2) Is the Sens seeting on the scope still correct? Which you've chanegd all the way up to 20?
That is very high.

Finally I am betting it is:

(3) Is the beam current being correct read by the scope? As Page-1 of the CRT display shows the
Curr Dens as received on the screen. Does that adjust when you adjust the brightness control?

--} Secondly, there is an adjustment that is made to the Curr Dens reading when the focusing screen
is in the beam path vs when it is out. Does the vale look reasonable in vs out?
--} I have had both bad signal from the main viewing screen and the adjustment from the focusing
screen in vs out.




On 19 Dec 2019 at 8:52, microscopy.listserver-at-gmail.c wrote:

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} Email: AFELL-at-2SPI.COM Name: Andrew Fell
}
} Organization: Structure Probe
}
} Title-Subject: [Filtered] TEM Film Issue
}
} Message: I am working on a JEOL 1200-EX that still uses film for
} imaging. Recently, the film started to come out completely
} under-exposed. It was discovered that the film exposure setting had
} changed to 20 out of 20. Since then, we have tried many different
} parameters and have had no positive results as far as useful images.
} After adjusting the FSE we have an image again, but now we are
} struggling to obtain a scale of contrast. Despite being in-focus and
} displaying layers of contrast on screen - the film continues to
} develop in a more black-and-white situation.
}
} We have gone through changing accelerating voltage, new film, new
} developing chem, exposure times, the full range of FSE, aperture
} adjustments and we are still hitting a wall. Has anyone dealt with
} this or (preferably) know how to resolve this issue? Thank you in
} advance!
}
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Richard E. Edelmann, Ph.D., Director
Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056
Ph: 513.529.5712 Fax: 513.529.4243
E-mail: edelmare-at-miamioh.edu
http://www.cami.muohio.edu


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From: crwinkler-at-ncsu.edu
Date: Mon, 10 Feb 2020 15:54:50 -0600
Subject: [Microscopy] Re: Ask-a-Microscopist: FIB sample preparation

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Aubrey,

Your Question: What are the best ways to avoid damaging or outright
destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for
atomic resolution STEM imaging?

I'm going to answer this in several parts.

You don't say what the material or the substrate is. I assume that it is a
semiconductor. If it is and the sample doesn't have to be site-specific,
then the best way to prepare a 20 nm thick epitaxial film and avoid Ga
damage is not to use the FIB at all.

1. The absolute best way to prepare your sample with no amorphous damage at
all is the Small Angle Cleavage Technique originally developed by John
McCaffrey and then modified quite a bit by John and myself. A major
disadvantage of this technique is that there is no amorphous damage and so
it can be difficult to focus and stigmate in a field emission TEM. I will
sometimes put a thin layer of ca carbon on the top surface just to have
something amorphous that I can use in the FFT to help focus and stigmata.
If you want, I can send you a detailed presentation on how to do the
technique. Just send a request to my work email:
(scott-dot-d-dot-walck2-dot-ctr-at-mail-dot-mil).

2. The next best way of preparing your sample would be low angle, low energy
Ar ion milling developed by Arpad Barna. Now, all the manufacturers of ion
mills have low angle, low energy capabilities. Arpad has several
publications out that show the amorphous damage at different energies and
angles.

3. OK, so you have a FIB and you don't want to learn how to make samples the
old fashioned way. You have to protect the top layer from ion damage before
putting on the ion beam Pt (or W) protective layer. You can put it on by
e-beam deposition. All you need is 200-250 nm thick to stop and ions from
hitting the top surface. Just don't let the ion beam hit the surface with
too high of a current or for very long (minimize the dosage to the surface
or you will erode the protective layer.) If your sample is not site specific
and you don't want to have a high Z material next to your very thin layer,
you should consider putting carbon down. Any easy way to do this is to use
a Sharpie(R) pen and coat the surface before you put it in the FIB. This
works, but can lead to open areas and what looks a bit stringy. A better
way is to use a carbon coater to put a uniform layer down. We have a Leica
ACE 600 and I just measured the thickness of carbon using a double carbon
thread and using it all up. It gave a thickness of 390 nm, which is plenty.
At any rate, putting the carbon layer next to your thin layer gives a better
contrast between the layer of interest and the protective top layer to see
it better. The high Z of Pt or W can make it difficult to see a very thin
layer at the top of the surface of your sample.

When preparing the sample by FIB, you will have an amorphous damage layer on
top and bottom with a thickness that is dependent on the energy of the beam
that you last use. The newest FIBs are capable of "polishing" with a 2 keV
(or lower) Ga beam to minimize the damage layer. It is done by exposing the
two surfaces at an angle of 3∞. Samples prepared this way are pretty good,
but remember, there is still an amorphous layer on both the top and bottom
surfaces.

There are also other ways of removing FIB damage from the surfaces of FIB
lamella using Ar ion milling. In decreasing order of cost:

-E.A. Fischione makes the NanoMill and the PicoMill, which use a
scanning Ar beam to polish the surfaces with low energy Ar. They have
presentations and product information on these tools.

-Several manufacturers of ion mills, specifically Gatan and
Technoorg Linda of recipes for removing damage using their tools. You
should contact them for papers and presentations on how to do that.

-Another technique that I patented while working at South Bay
Technology is the Plasma Trimming method, which uses the shape of the FIB
sample and a bias to create a field that causes ions from a plasma to polish
the two surface of the sample. The plasma was generating using Ar gas in a
plasma cleaner. This patent is now owned by Ted Pella, Inc. I can provide
a Plasma Trimming presentation upon request.
(scott-dot-d-dot-walck2-dot-ctr-at-mail-dot-mil)

BTW, you can estimate the amount of damage and depth for different ions,
energies, and angles by using SRIM and TRIM calculations. See www.srim.org
for more information and to download the software and manual.

I hope that this helps. I'm sure it is more than you need.

-Scott






-----Original Message-----
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Sent: Friday, February 7, 2020 11:55 AM
To: s.walck-at-comcast.net

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Forwarded from "Ask a Microscopist"
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Name: Aubrey Penn
Email: anpenn-at-ncsu.edu

Hello Aubrey,

Good to see you on the listserv!

In regards to your question, Valery and Scott gave you some good
advice. I've always referred students to the following paper:
https://www.sciencedirect.com/science/article/abs/pii/S030439911200006X
I can give you a copy the next time I see you.

There are a lot of ways to skin a cat, as they say, but it can be
challenging to get good FIB samples if you're used to the results
generated by tripod polishing and low energy, angle ion milling. The
best results I've seen are from FIB samples prepared in a similar
manner as in the paper above, followed by a cleanup in a low energy
ion mill (Fischione, Gatan, Technoorg-Linda...all are fine). However,
the ion mill cleanup is not magic. You can start to etch your liftout
if the angle and voltages are not ideal, or if you mill for too long
to compensate for a thicker liftout.

Depending on the materials, you may also be able to dip the liftout in
a dilute acid or base to thin the liftout, or use a flash
electropolishing setup like the one they have at PNNL to reduce the
liftout thickness and remove damage layers. Regardless of what
post-FIB step you use, you need to do your best to create a thin, flat
liftout with a minimal amount of sputter redep in the first place, and
that's where that paper gives some valuable tips.

Good luck,
Chris


On Fri, Feb 7, 2020 at 10:52 AM {oshel1pe-at-cmich.edu} wrote:
}
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} Name: Aubrey Penn
} Email: anpenn-at-ncsu.edu
} Subject: FIB sample preparation
} Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging?
}
} I'm not a FIB expert, but there are plenty on the microscopy list. There will probably be other people who would like to know the answer to this question, too, so please reply both directly to Penn and to the list.
}
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} Microscopy Society of America
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--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
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Cell: 267-496-0587


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Subject: [Microscopy] viaWWW:We are hiring! Electron Microscopist / Imaging Specialist

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From: microscopy.listserver-at-gmail.com
Date: Sun, 22 Mar 2020 12:49:07 -0500
Subject: [Microscopy] viaWWW:Covid-19 virus

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Email: xbren-at-uw.edu Name: Shirley Ren

Organization: University of Washington

Title-Subject: [Filtered] Covid-19 virus
Message: Dear Colleagues,
Has someone found the virus under TEM? Is it possible to do RNA-FISH on thick sections (Epoxy
embedded block)?
Many thanks,

Shirley


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From: microscopy.listserver-at-gmail.com
Date: Tue, 24 Mar 2020 08:31:14 -0500
Subject: [Microscopy] Fwd: Re: Fwd: Re: viaWWW: Teaching SEM Online

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X-from: Beck, Stephen J. {Stephen.Beck-at-ncc.edu}

Hi David,

Thanks for the reply. I’m not sure how I could get samples to you. My students were just working on
getting samples prepared. My microbial/Paramecium prep was canceled by the corona virus. My students
and I can’t even get on campus at present.
Do you have any “stock” biological samples on hand? Can you even get to your own scopes? My students
have required samples to image but I’m sure I’ll have to cut that back given the current situation.
They would image 2 soft tissues, a cryofractured soft tissue, hard tissue, botanical sample (leaf
stomates), pollen, microbial/Paramecium, insect, diatoms.
Perhaps I could do a trial demo with you initially?

Thanks,

Steve

Stephen J. Beck
Professor
Coordinator, Bio-Imaging Center
Electron Microscopy Department of Biology
Nassau Community College
Garden City, NY 11530

Sent from my iPhone - thanks Steve (1955-2011)!

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} Hi Stephen,
}
} We have a couple of our SEMs connected to the internet for remote access. Through this interface,
} the microscope is completely controllable from your own PC. We can load your samples and then you or
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} Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck
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} Organization: Nassau Community College
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} Title-Subject: [Filtered] Teaching SEM Online
}
} Message: Dear Colleagues,
} I am teaching my SEM course this semester and like many institutions, we are trying to teach
} online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam
} online,
} however, after that we need to get on the SEM to image the required samples.
} Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
} this is impossible - I haven't received the courtesy of a response yet!
}
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From: microscopy.listserver-at-gmail.com
Date: Tue, 24 Mar 2020 08:35:23 -0500
Subject: [Microscopy] viaWWW:I'd like to help with Corona virus research

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Email: drk-at-shcc.org Name: Doug Keene

Organization: Shriners Hospital for Childern

Title-Subject: [Filtered] I'd like to help with Corona virus research

Message: Hello Everyone,

I'd like to apply my talents in Microscopy (TEM, SEM, Confocal, Micro-CT) to the Corona pandemic.
How do I get the word out that I would like to contribute?

Thanks,

Doug Keene
Shriner Hospital for Children
Portland, Oregon
503-819-3600 (cell)

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From: microscopy.listserver-at-gmail.com
Date: Tue, 24 Mar 2020 08:26:44 -0500
Subject: [Microscopy] Fwd: Re: Fwd: Re: viaWWW: Teaching SEM Online

Contents Retrieved from Microscopy Listserver Archives
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X-from: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

It seems that you replied to the entire list.
Since you did, there should be many scopes in many labs that should be able to help. The FEI scopes
are setup to use UltraVNC as part of their RAPID support service. It can be used just as well by lab
staff and clients for their own purposes. (In fact, I have used it more for my own benefit than FEI
has ever used it for theirs.) It would need to be setup properly with a single computer controlling
the computer. Multiple others might be able to watch along.
I would offer to help, but I am in the materials science area and would barely know what I am
looking at. We also do not have samples handy for demo purposes. I have a counterpart that would
have such expertise and samples, but she runs a Hitachi SEM and it is not setup for remote control
or viewing.
Warren Straszheim, Ph.D., manager
Materials Analysis and Research Lab
Iowa State University
515-294-8187


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, March 24,
2020 8:32 AM
To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

X-from: Beck, Stephen J. {Stephen.Beck-at-ncc.edu}

Hi David,

Thanks for the reply. I’m not sure how I could get samples to you. My students were just working on
getting samples prepared. My microbial/Paramecium prep was canceled by the corona virus. My students
and I can’t even get on campus at present.
Do you have any “stock” biological samples on hand? Can you even get to your own scopes? My students
have required samples to image but I’m sure I’ll have to cut that back given the current situation.
They would image 2 soft tissues, a cryofractured soft tissue, hard tissue, botanical sample (leaf
stomates), pollen, microbial/Paramecium, insect, diatoms.
Perhaps I could do a trial demo with you initially?

Thanks,

Steve

Stephen J. Beck
Professor
Coordinator, Bio-Imaging Center
Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530

Sent from my iPhone - thanks Steve (1955-2011)!

} On Mar 22, 2020, at 12:46 PM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
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} X-from: David Huskisson {david-at-emanalytical.co.uk}
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} Hi Stephen,
}
} We have a couple of our SEMs connected to the internet for remote
} access. Through this interface, the microscope is completely
} controllable from your own PC. We can load your samples and then you or your students would be able to take control of the instrument and practice from your own homes.
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} Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name:
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} Organization: Nassau Community College
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} Title-Subject: [Filtered] Teaching SEM Online
}
} Message: Dear Colleagues,
} I am teaching my SEM course this semester and like many institutions, we are trying to teach
} online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam
} online,
} however, after that we need to get on the SEM to image the required samples.
} Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
} this is impossible - I haven't received the courtesy of a response yet!
}
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From: microscopy.listserver-at-gmail.com
Date: Tue, 24 Mar 2020 08:33:05 -0500
Subject: [Microscopy] Fwd: E coli capsule polysaccharide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: hiro uryu {hiro.uryu-at-emscopic.com}


Dear List,

I have tried to visualize E cloi capsular polysaccharide by following the protocol from the book
chapter "Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell" by Sven
Hammerschmidt and Manfred Rohde from the published book entitled "Streptococcus pneumoniae, Method
and protocol" published by Humana Press. I compared alcian blue and ruthenium red in the presence or
absence of lysine.

At least in this first trial, ruthenium red in the presence of lysine appeared to be superb among
all conditions tested in our study setup. However, based on the EM analysis, I sensed that the
preservation of CPS is suboptimal and suggesting room for improvement.

I was wondering if CPS experts could give me any suggestions about how I can improve the
presentation of CPS. Many thanks,

Sincerely,
Hiro
------
Kunihiro Uryu,


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From: microscopy.listserver-at-gmail.com
Date: Tue, 24 Mar 2020 09:09:21 -0500
Subject: [Microscopy] Fwd: Re: Fwd: E coli capsule polysaccharide

Contents Retrieved from Microscopy Listserver Archives
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X-from: Rob Mesman {R.Mesman-at-science.ru.nl}

Dear Hiro,
I would recommend checking the review Ruthenium Red and the Bacterial Glycocaly by Fassel et al.
(1999) https://doi.org/10.3109/10520299909047974
it compares several different fixation and staining protocols with indications of what works best
for which type of CPS.
best regards!
Rob

---
Dr. Rob Mesman
Post-Doc
Department of Microbiology
Faculty of Science
Radboud University Nijmegen
Heyendaalseweg 135
NL-6525 AJ Nijmegen
The Netherlands

On 2020-03-24 14:43, microscopy.listserver-at-gmail.com wrote:
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} X-from: hiro uryu {hiro.uryu-at-emscopic.com}
}
}
} Dear List,
}
} I have tried to visualize E cloi capsular polysaccharide by following
} the protocol from the book
} chapter "Electron Microscopy to Study the Fine Structure of the
} Pneumococcal Cell" by Sven
} Hammerschmidt and Manfred Rohde from the published book entitled
} "Streptococcus pneumoniae, Method
} and protocol" published by Humana Press. I compared alcian blue and
} ruthenium red in the presence or
} absence of lysine.
}
} At least in this first trial, ruthenium red in the presence of lysine
} appeared to be superb among
} all conditions tested in our study setup. However, based on the EM
} analysis, I sensed that the
} preservation of CPS is suboptimal and suggesting room for improvement.
}
} I was wondering if CPS experts could give me any suggestions about how
} I can improve the
} presentation of CPS. Many thanks,
}
} Sincerely,
} Hiro
} ------
} Kunihiro Uryu,
}
}
} ==============================Original
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From: gilpin-at-purdue.edu
Date: Tue, 24 Mar 2020 09:14:41 -0500
Subject: [Microscopy] remote control of FEI TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi everyone,
I have a full FEI remote package that we bought with our Themis Z but I wondered if there was another solution to remote TEM that people are using. I have a spare set of hand panels already.

Chris
He/Him/His
Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility



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From: wij.muss-at-aon.at
Date: Tue, 24 Mar 2020 09:57:52 -0500
Subject: [Microscopy] Re: E coli capsule polysaccharide

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Dr. Kunihiro Uryu,
seconding Dr. Rob Mesman: fixation conditions…

Unfortunately not in possession of the protocol Dr. Kunihiro Uryu quoted*) only „active working“ colleagues might have the protocol in their collection, read and apply or have an answer for your request.

*) only for convenience:
https://www.ncbi.nlm.nih.gov/pubmed/30929202
Methods Mol Biol. 2019;1968:13-33. doi: 10.1007/978-1-4939-9199-0_2.

Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell.
Hammerschmidt S1, Rohde M2.
Author information
Abstract
Electron microscopy allows for studying bacterial ultrastructure at high resolutions. Two types of electron microscopes are used for this purpose. The transmission electron microscope allows for access to inner bacterial ultrastructure when imaging ultrathin sections as well as cell wall-attached structures by negative staining, whereas scanning electron microscopy allows for the detection of structures on the bacterial cell surface alone or to study the interplay between pneumococci and their host cells. This chapter deals with recommendations for well-adapted methodologies to examine pneumococcal ultrastructure in detail. Especially, we focus on the preservation of the pneumococcal capsular polysaccharide, which represents an important virulence factor of pneumococci. Since capsules are highly hydrated structures, the introduction of a new fixation protocol involving lysine acetate, ruthenium red, and osmium (LRR fixation) results in a very well-preserved capsular structure in such a way that the amount of capsular material bound on the bacterial surface can be compared within different serotypes. In our method, capsular ultrastructure is preserved without the need for serotype-specific antibodies, which have been used in other studies to preserve the pneumococcal capsule. In addition, the new LRR fixation allows for studying the presence or absence of capsular material during adhesion and invasion of pneumococci on epithelial or endothelial host cells in cell culture experiments.
KEYWORDS: Critical point drying; Cryo-FESEM; Field emission scanning electron microscopy; Infection; LRR embedding; LRWhite resin; Pneumococcal capsule; Pneumococci; Transmission electron microscopy
PMID: 30929202
DOI: 10.1007/978-1-4939-9199-0_2
[Indexed for MEDLINE]
⇒SPRINGER:
https://link.springer.com/protocol/10.1007%2F978-1-4939-9199-0_2

It would be interesting whether you tried "additives" like e.g. TA, La+++ and / or PPD (at least for a part of your specimens) with regard to fixation of the bacteria**)
(ok, I know that E. coli is something different from....but you might have a look into my Poster
https://www.researchgate.net/publication/215824406_Purpura_fulminans_ultrastructural_findings_by_transmission_electron_microscopy_in_four_cases_with_special_reference_to_fixation_of_skin_biopsies
and the oral presentation, uploaded into RG
https://www.researchgate.net/publication/281120633_Oral_Lecture_PURPURA_FULMINANS_Ultrastructural_findings_by_Transmission_Electron_Microscopy_in_4_cases_with_special_reference_to_fixation_of_skin_biopsies

Eventually you might have tried also the variation (".....substituting ruthenium red with a 0.5% alcian blue pyridine variant (pH 7.2).), cf.
https://www.researchgate.net/publication/313875937_Molecular_characterization_of_invasive_capsule_null_Neisseria_meningitidis_in_South_Africa
https://www.researchgate.net/figure/Transmission-electron-micrographs-showing-the-presence-of-surface-capsular-polysaccharide

Beware of Covid-19/SARS-CoV-2 infection,
keep distance and
STAY WELL,

best wishes AND best regards

Wolfgang Muß (MUSS)
Retired Member of MSA
SALZBURG, Austria


**) what I wanted to say with this is only pointing to other possibilities for retaining otherwise eluted "cellular or matricial substrate(s) in preparation for TEM....
(Sorry if I bothered anyone in this time of obviously global crisis)





======================================================================
Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com]
Gesendet: Dienstag, 24. März 2020 15:10
An: wij.muss-at-aon.at
Betreff: [Microscopy] Re: E coli capsule polysaccharide

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X-from: Rob Mesman {R.Mesman-at-science.ru.nl}

Dear Hiro,
I would recommend checking the review Ruthenium Red and the Bacterial Glycocaly [Glycocalyx] by Fassel et al.
(1999) https://doi.org/10.3109/10520299909047974
it compares several different fixation and staining protocols with indications of what works best for which type of CPS.
best regards!
Rob

---
Dr. Rob Mesman
Post-Doc
Department of Microbiology
Faculty of Science
Radboud University Nijmegen
Heyendaalseweg 135
NL-6525 AJ Nijmegen
The Netherlands

On 2020-03-24 14:43, microscopy.listserver-at-gmail.com wrote:
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} X-from: hiro uryu {hiro.uryu-at-emscopic.com}
}
}
} Dear List,
}
} I have tried to visualize E cloi capsular polysaccharide by following
} the protocol from the book chapter "Electron Microscopy to Study the
} Fine Structure of the Pneumococcal Cell" by Sven Hammerschmidt and
} Manfred Rohde from the published book entitled "Streptococcus
} pneumoniae, Method and protocol" published by Humana Press. I
} compared alcian blue and ruthenium red in the presence or absence
} of lysine.
}
} At least in this first trial, ruthenium red in the presence of lysine
} appeared to be superb among all conditions tested in our
} study setup. However, based on the EM analysis, I sensed that the
} preservation of CPS is suboptimal and suggesting room for improvement.
}
} I was wondering if CPS experts could give me any suggestions about how
} I can improve the presentation of CPS. Many thanks,
}
} Sincerely,
} Hiro
} ------
} ,
}
}
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23, 20 -- Subject: [Microscopy] Re: E coli capsule polysaccharide
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From: matthew.weyland-at-monash.edu
Date: Tue, 24 Mar 2020 20:43:06 -0500
Subject: [Microscopy] Re: remote control of FEI TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Chris,

While there are many software solutions for sharing desktops, they all
fail a similar hurdle - you have to have your microscope PC connected
to the network directly. This is NOT a good idea as your Themis computer
will not be protected from the internet.

Over the last few months I've been suing a different solution on our
"Classic" Titan cubed. Making use of KVM over IP gear. These consist of
sender boxes that have HDMI and USB (and audio) inputs that connect (and
can be powered by) Cat5 cables to your network. Then any number of
receiver boxes can be used with HDMI/USB outputs anywhere you have a
network port. These systems have MANY advantages over the FEI remote
software;

1) They are FAST - they do real time up to 4k - not just 10fps you will
be lucky to get over VNC
2) There is no intrinsic connection between the microscope and the
network - as such there is no security issue (except if someone managed
to work out your IP/passwords for the boxes, or put a virus on a USB
stick - but this is just be careful..)
3) You can have multiple receivers for every sender - so many people can
watch each instrument.
4) USB can be used for the hand panels, keyboard and mice for the computers.
5) Partner these with HDMI switches behind monitors and you can switch
between local/remote super quickly.
6) They are microscope/instrument agnostic - if the instrument uses USB
and HDMI etc it will work.

Downsides;

1) They work with little configuration inside a subnet (such an
individual building - our situation where we have been running the
instrument from multiple different locations due to building work) but
if you are outside the subnet you'll need some help from your network
people.
2) The boxes cost - on the order of $500 for each box. But compared to a
microscope..
3) And you'll need one sender for each screen.

I've been using boxes made by Kramer (I have NO connection to this
company) but there are many manufacturers (Lindy, geffen etc). They are
mostly used is point of sale displays (all those screens at malls and
movie theatres) so they are pretty ubiquitous. I have three boxes (left
screen, right screen and accessory screen which runs Gatan, Bruker,
EMPAD AND support computer through a remote controlled switch). This has
worked really well. Let me know if you have any follow up questions.

Cheers

Matthew

--
Dr Matthew Weyland
Joint Deputy Director - Monash Centre for Electron Microscopy
Associate Professor - Department of Materials Science and Engineering

Monash Centre for Electron Microscopy
10 Innovation Walk, Clayton Campus
Monash University
Clayton VIC 3800 Australia

T: +61 3 9905 9026
E: matthew.weyland-at-monash.edu
mcem.monash.edu



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hi everyone,
I have a full FEI remote package that we bought with our Themis Z but I
wondered if there was another solution to remote TEM that people are
using. I have a spare set of hand panels already.

Chris
He/Him/His
Christopher J. Gilpin Ph.D.
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility



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19, 49 -- From: Matthew Weyland {matthew.weyland-at-monash.edu}
19, 49 -- Subject: [Microscopy] Re: remote control of FEI TEM
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From: bradford.ross-at-botany.ubc.ca
Date: Tue, 11 Feb 2020 14:26:15 -0600
Subject: [Microscopy] Core Facility Research Tech Position Available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Listers,

After 12+ great years at the UBC BioImaging Facility, I am moving on to a new position in the
private sector next month! As such, my position here is available now and has been posted to the UBC
HR website:

https://www.hr.ubc.ca/careers-postings/staff-s.php Posting ID: 36809

This is a great opportunity for someone with experience in various advanced TEM techniques including
electron tomography, cryo TEM, HRTEM, and their associated sample prep and image processing
techniques. We have also just moved the facility into brand new lab space, so the world will be your
oyster! There is opportunity to work with our SEM and optical microscope instruments as well.

Concurrently, we are also looking for an optical microscopy specialist to run our three laser
scanning confocal instruments, including an Olympus Multiphoton system, a Perkin-Elmer spinning
disk, and their associated equipment. https://www.hr.ubc.ca/careers-postings/staff-s.php Posting ID:
36794

I am happy to answer other questions about the TEM tech position, but please direct any formal
inquiries to our facility manager, Miki Fujita. BIF.manager-at-ubc.ca
Cheers,
Bradford Ross
Electron Microscopy Technician
BioImaging Facility
University of British Columbia
Biological Sciences Rm. 0120
6270 University Blvd. Vancouver, B.C. V6T 1Z4
phone 604-822-6996
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From: koeck-at-kth.se
Date: Tue, 18 Feb 2020 09:24:20 -0600
Subject: [Microscopy] SAD-aperture assembly

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

​Hi.


Does anybody happen to have an SAD-aperture assembly (aka field limiting aperture assembly,
manual version) for a Jeol JEM2100F lying outside the microscope at the moment?


I would be really interested in a photo of it with some measurements,
mainly the diameter and length of the rod.


Alternatively, a sketch with dimensions would also be great.


All the best,


Philip


PS: I'm trying to avoid opening up the microscope and getting drawings from Jeol is a lengthy procedure.


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From: xinran.liu-at-yale.edu
Date: Wed, 25 Mar 2020 08:44:08 -0500
Subject: [Microscopy] EM research associate position at Yale University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Collogues,

The Coln-Ramos lab at Yale University is looking for a research associate to provide ongoing
electron microscopy work for a variety of projects in the lab. Under the direction of the Principal
Investigator and working in collaboration with laboratory personnel, this position will be in charge
of the design, collection, optimization and interpretation of electron microscopy research in the
Coln-Ramos lab as it pertains to the ultrastructure of C. elegans nervous system. The position will
prepare and process C. elegans worms for electron microscopy to better understand the function and
structure of the C. elegans nervous system. More information about the position can be found here:
https://www.nature.com/naturecareers/job/electron-microscopy-research-associate-yale-university-715443

Thanks for your attention.


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From: microscopy.listserver-at-gmail.com
Date: Wed, 19 Feb 2020 05:18:43 -0600
Subject: [Microscopy] viaWWW: Postdoctoral position at the Ames Laboratory

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Email: tprozoro-at-ameslab.gov Name: Tanya Prozorov

Organization: Ames Laboratory

Title-Subject: [Filtered] Postdoctoral position at the Ames Laboratory

Message: The Division of Materials Science and Engineering (DMSE) at the Ames Laboratory, a
Department of Energy National Laboratory affiliated with Iowa State University, is searching for a
qualified Postdoctoral Research Associate.

We are looking for a motivated postdoctoral research associate to work on two projects focused on
characterization of structure and liquid phase dynamics of beam-sensitive materials by using
electron microscopy in-situ. The first project involves spatio-chemical characterization of
polymer-nanoparticle complexes and DNA origami nanostructures in liquid phase. The second project
involves monitoring cation mobility in low-contrast layered minerals in liquid phase and in-situ. We
are looking for applicants with hands-on experience using aberration-corrected scanning transmission
electron microscopy (S/TEM), electron energy loss spectroscopy (EELS), and energy dispersive
spectroscopy (EDS).
More details and application instructions can be found here:
https://isu.wd1.myworkdayjobs.com/en-US/IowaStateJobs/job/Ames-IA/Postdoctoral-Research-Associate---Ames-Laboratory_R1870

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From: xinran.liu-at-yale.edu
Date: Wed, 25 Mar 2020 08:48:34 -0500
Subject: [Microscopy] Re: EM research associate position at Yale University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,
I apologize for a job posting that appears containing an invalid link. Thanks for those of you who
brought it to my attention. Here is the link to the Yale HR website, and it works.
https://sjobs.brassring.com/TGnewUI/Search/Home/Home?partnerid=25053&siteid=5248#jobDetails=1405083_5248.

Thanks you for your attention.

Xinran Nick Liu, M.D. & Ph.D.
Director, Center for Cellular and Molecular Imaging
Bio & Cryo Electron Microscopy Core Facilities
Yale University School of Medicine
Office: (203) 785-4050
Lab: (203) 785-5390
http://medicine.yale.edu/ccmi/em




On 2/18/20, 1:46 PM, "Liu, Xinran" {xinran.liu-at-yale.edu} wrote:

Dear Collogues,
The Colón-Ramos lab at Yale University is looking for a research associate to provide
ongoing electron microscopy work for a variety of projects in the lab. Under the direction of the
Principal Investigator and working in collaboration with laboratory personnel, this position will be
in charge of the design, collection, optimization and interpretation of electron microscopy research
in the Colón-Ramos lab as it pertains to the ultrastructure of C. elegans nervous system. The
position will prepare and process C. elegans worms for electron microscopy to better understand the
function and structure of the C. elegans nervous system. More information about the position can be
found here:
https://www.nature.com/naturecareers/job/electron-microscopy-research-associate-yale-university-715443
Thanks for your attention.


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11, 77 -- From: "Liu, Xinran" {xinran.liu-at-yale.edu}
11, 77 -- To: "'Microscopy-at-microscopy.com'" {Microscopy-at-microscopy.com}
11, 77 -- Subject: Re: EM research associate position at Yale University
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From: microscopy.listserver-at-gmail.com
Date: Wed, 19 Feb 2020 14:44:58 -0600
Subject: [Microscopy] viaWWW:Stitching confocal volumes of organ in 3 dimensions

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Email: dkc25-at-cam.ac.uk Name: Darran Clements

Organization: University of Cambridge

Title-Subject: [Filtered] Stitching confocal volumes of organ in 3 dimensions

Message: Hi All,
just wanted to throw out a general question to the list. We are currently considering embarking on a
project where we have been asked to reconstruct an organ from mosaics of confocal volumes in serial
sections. We can stitch volumes in each section together easily enough, however, I wanted to ask if
anyone else has experience of then stitching these kinds of volumes together to reconstruct the
whole organ volume, and if so, could they impart some advice on what they found works best.

We are looking at slices of tissue around 100 microns thick and around 80-100 of these volumes
stitched together, then stitching multiple of these volume mosaics, one above the other, to
reconstruct the tissue.

We're mainly looking at the software aspect of this but of course, tangential replies and
alternative ideas are more than welcome too.

Many thanks
Darran

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From: bozhilov-at-ucr.edu
Date: Wed, 25 Mar 2020 08:52:53 -0500
Subject: [Microscopy] Re: question about EDS in SEM

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By the way, the solid angle would be the active area of the detector divided by the square of the
distance to specimen.

Krassimir.


} On Feb 19, 2020, at 2:45 PM, nizets2-at-yahoo.com wrote:
}
}
}
}
} ----------------------------------------------------------------------------
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} Dear colleagues,
}
} I'd have a simple question regarding the detector area of EDX (EDS) systems in SEM:
} Clearly the largest the detector, the highest the sensitivity for everything else equal but what if everything else was NOT equal?
}
} One clear example:
}
} - detector 1: 30mm², optimal distance to pole piece 10mm
} - detector 2: 150mm², optimal distance to pole piece 15mm
}
} Here the largest detector is also the furthest!
}
} I don't know how to appreciate the influence of the 2 factors, detector area and work distance, on the final sensitivity of the system.
} In other words, is the extra $$$ for the larger detector worth it if the work distance is at the same time increased?
}
} Many thanks in advance.
} Stephane
}
}
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From: microscopy.listserver-at-gmail.com
Date: Wed, 25 Mar 2020 08:54:35 -0500
Subject: [Microscopy] Fwd: Traceable Standard Specimen for TEM calibration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Erico Freitas {ericotadeu-at-ufmg.br}


Dear all,


We are looking after a traceable standard specimen for TEM calibration. We had a MAG*I*CAL sample
that got damaged, and it is in a temporary backorder at the EMSDiasum website.

Does any of you know and have used another traceable standard specimen for TEM calibration?


Thank you,

--
Erico Freitas

Physicist/Microscopist at Center of Microscopy
Universidade Federal de Minas Gerais (UFMG)
Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901.
+55-31-3409-7573
+55-31-3409-7575

Coordinator:Transmission Electron Microscopy laboratory

CV Lattes: *http://lattes.cnpq.br/8786127123101199*
{https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#}
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From: zaluzec-at-microscopy.com
Date: Wed, 25 Mar 2020 09:00:46 -0500
Subject: [Microscopy] Re: question about EDS in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Stephane

Go to this WWW site. It is an on-line solid angle calculator for XEDS systems.

http://www.zaluzec.com/NJZTools/

It will give you an unbiased calculation of solid angles.

Importantly I must contradict Krassimir, Area/distance^2 is incorrect, that is an approximation
which quickly breaks down for large detectors and small distances.

On that Solid Angle WWW site is also a link to a paper you might want to read.

Cheers

Nestor
Your Friendly Neighborhood SysOp

On 2/20/20 9:40 AM, nizets2-at-yahoo.com wrote:
} ----------------------------------------------------------------------------
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} Dear colleagues,
}
} I'd have a simple question regarding the detector area of EDX (EDS) systems in SEM:
} Clearly the largest the detector, the highest the sensitivity for everything else equal but what if everything else was NOT equal?
}
} One clear example:
}
} - detector 1: 30mm², optimal distance to pole piece 10mm
} - detector 2: 150mm², optimal distance to pole piece 15mm
}
} Here the largest detector is also the furthest!
}
} I don't know how to appreciate the influence of the 2 factors, detector area and work distance, on the final sensitivity of the system.
} In other words, is the extra $$$ for the larger detector worth it if the work distance is at the same time increased?
}
} Many thanks in advance.
} Stephane
}
}
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From: wesaia-at-iastate.edu
Date: Thu, 20 Feb 2020 09:53:12 -0600
Subject: [Microscopy] question about EDS in SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Let me be sure I understand your question correctly. Your first option of a 30-mm2 detector would
allow a 10-mm working distance. Your second option of a 150mm2 detector would require a 15-mm
working distance to the pole piece.
That does not say anything about the sample to detector distance which is required to calculate the
solid angle. The 15-mm working distance might be your better option.
That is similar to the situation we ran into. I wished I had posed the question to the list 9 years
ago. - We had been using a 10mm2 detector on a Hitachi 2460N which was setup for a 25-mm working
distance for the eucentric height and analytical distance. We were able to bring the EDS detector in
below the pole piece so that it was quite close to the sample and gave us a pretty good solid angle.
It did mean that the EDS detector was the first thing in harm's way. A user scratched up one of our
aluminum sample platens when they raised it into the end of the detector then proceeded to move the
sample around. - We upgraded to a FEI Quanta with field emission. It's eucentric height was 10-mm
which was better for imaging. We knew we wanted to get a large detector in order to maintain as much
image resolution as possible when doing EDS so we opted for an 80-mm2 detector figuring we would get
8x more counts at the same beam current. It turned out that we only got about 3x more. We had
ordered the system configured to work at 10-mm working distance. With the design of the pole piece,
that meant our detector had to stand about 70% further off than before. So we gained 8x from the
detector area but lost almost 3x due to the greater distance.
I have wondered about redoing our system to operate at 15-mm working distance and bring the detector
in much closer. It would greatly improve our solid angle. If I was only doing EDS, I would probably
go for it. It would mean different operating conditions for EDS versus just imaging. That might
confuse some of our many users. We also have WDS installed, and I understand it is aimed at 10-mm.
So, I will probably just leave things as they are and bring up the current a bit more.
Now to the other question of performance and resolution as a function of size. It is true that all
other things being equal, you may give up a little energy resolution by going to a bigger detector.
It is harder to find a crystal of the same, good resolution when it has to be 8x larger, but it can
be done. They will probably charge a premium for it. You probably want to compare spectra on your
real samples.
I choose to back off on the process time to push more counts at the same deadtime. So, I am already
sacrificing some resolution. I am glad that I started with a better detector. There are times that I
want for better resolution so I increase the process time and cut the count rate - or I switch over
to the WDS.
Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
X-from: nizets2-at-yahoo.com {nizets2-at-yahoo.com} Sent: Wednesday, February 19, 2020 4:41 PM
To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

Dear colleagues,

I'd have a simple question regarding the detector area of EDX (EDS) systems in SEM:
Clearly the largest the detector, the highest the sensitivity for everything else equal but what if
everything else was NOT equal?

One clear example:

- detector 1: 30mm², optimal distance to pole piece 10mm
- detector 2: 150mm², optimal distance to pole piece 15mm

Here the largest detector is also the furthest!

I don't know how to appreciate the influence of the 2 factors, detector area and work distance, on
the final sensitivity of the system.
In other words, is the extra $$$ for the larger detector worth it if the work distance is at the
same time increased?

Many thanks in advance.
Stephane

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From: microscopy.listserver-at-gmail.com
Date: Fri, 21 Feb 2020 16:24:35 -0600
Subject: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM sample preparation

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Email: arunas.mesceriakovas-at-uef.fi Name: Arnas Meeriakovas

Organization: University of Eastern Finland

Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM sample preparation

Message: Hello,


I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use TEM to check the
morphology/size distribution of the sample i.e. individual particles or agglomerates. I have
prepared the samples by dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.

I am aware that due to drying of the solvent from the TEM grid, the carbon dots that are present in
the drop are forced together and may form agglomerates.
I would like to eliminate/minimize the agglomerate formation in the sample preparation process as it
is influencing my analysis which is used to evaluate the synthesis method (formation of single
particles/formation of agglomerates).

What are the possible methods I could use in my sample prep, to get the least possible particle
agglomeration?

Login Host: 193.167.228.180
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From: Frank Karl :      frank_karl-at-ardl.com
Date: Mon, 24 Feb 2020 20:43:06 -0500
Subject: RE: [Microscopy] Fwd: Traceable Standard Specimen for TEM calibration

Contents Retrieved from Microscopy Listserver Archives
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We searched and after several years the answer was clearly no.
We settled our traceable TEM calibration problem by calibrating with a replica grating and then averaging the measurement of 200 particles of a NIST traceable nanospheres. We selected the 200nm (actually203nm) traceable spheres and typically get numbers within 5%. There are smaller traceable spheres, but the 200 have the smallest Std deviation and expanded uncertainty,

Please let me know if you find a better solution.


Stay safe..

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305



Orignal Message:


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X-from: Erico Freitas {ericotadeu-at-ufmg.br}


Dear all,


We are looking after a traceable standard specimen for TEM calibration.
We had a MAG*I*CAL sample that got damaged, and it is in a temporary backorder at the EMSDiasum website.

Does any of you know and have used another traceable standard specimen for TEM calibration?


Thank you,

--
Erico Freitas

Physicist/Microscopist at Center of Microscopy
Universidade Federal de Minas Gerais (UFMG)
Av. Antnio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil.
ZIP Code 31270-901.
+55-31-3409-7573
+55-31-3409-7575

Coordinator:Transmission Electron Microscopy laboratory

CV Lattes: *http://lattes.cnpq.br/8786127123101199*
{https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#}



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From: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday,
Date: Mon, 24 Feb 2020 14:52:45 +0000
Subject: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM sample preparation

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Email: arunas.mesceriakovas-at-uef.fi
Name: Arnas Meeriakovas

Organization: University of Eastern Finland

Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM sample preparation

Message: Hello,


I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use TEM to check the
morphology/size distribution of the sample i.e. individual particles or agglomerates. I have
prepared the samples by dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.

I am aware that due to drying of the solvent from the TEM grid, the carbon dots that are present in
the drop are forced together and may form agglomerates.
I would like to eliminate/minimize the agglomerate formation in the sample preparation process as it
is influencing my analysis which is used to evaluate the synthesis method (formation of single
particles/formation of agglomerates).

What are the possible methods I could use in my sample prep, to get the least possible particle
agglomeration?

Login Host: 193.167.228.180
Listserver Email Form V - 20120416
---------------------------------------------------------------------------


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From: mdelann1-at-jhmi.edu
Date: Tue, 25 Feb 2020 12:13:12 -0600
Subject: [Microscopy] UA

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Arunas,
I forgot to mention UA is incompatible with phosphate and will precipitate in its presence. If you
use it then briefly rinse in DH2O before the negative staining (after sample adsorbtion).
Good Luck,
Michael Delannoy
Johns Hopkins SOM Microscope Facility

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From: petereaton-at-hotmail.com
Date: Wed, 26 Feb 2020 05:21:41 -0600
Subject: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM sample preparation

Contents Retrieved from Microscopy Listserver Archives
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Dear Arunas,
You probably already considered this, but whatever you see on TEM is not going to be representative
of what's happening in solution, irrespective of preparation method. (Except, maybe
freezing/Cryo_TEM?). If what you want to know is how they are dispersed in solution, then DLS or a
similar light scattering technique might be best. 20nm particles are well within the size range
that's possible. Although, I suppose there will be relatively little contrast due to the material,
it should be feasible. In any case, it's a very quick method to try.
Good luck,
Pete.
____________________________________________________________________________
Atomic Force Microscopy
Dr Peter Eaton and Dr Paul West
Available through all good bookshops, or direct from Oxford University Press at:
http://ukcatalogue.oup.com/product/9780199570454.do http://afmhelp.com


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Email: arunas.mesceriakovas-at-uef.fi Name: Arnas Meeriakovas

Organization: University of Eastern Finland

Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM
sample preparation

Message: Hello,


I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use
TEM to check the morphology/size distribution of the sample i.e.
individual particles or agglomerates. I have prepared the samples by
dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.

I am aware that due to drying of the solvent from the TEM grid, the
carbon dots that are present in the drop are forced together and may
form agglomerates.
I would like to eliminate/minimize the agglomerate formation in the
sample preparation process as it is influencing my analysis which is
used to evaluate the synthesis method (formation of single
particles/formation of agglomerates).

What are the possible methods I could use in my sample prep, to get the
least possible particle agglomeration?

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23, 77 -- sample preparation
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From: vray-at-partbeamsystech.com
Date: Wed, 26 Feb 2020 08:43:45 -0600
Subject: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Look up "Ammonium Laurate Surfactant for Cleaner Deposition of Carbon Nanotubes" by Hannah Nisson,
DOI:10.1021/acs.langmuir.5b01175 on using surfactants to avoid agglomeration during TEM sample prep.

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 2/26/2020 6:21 AM, petereaton-at-hotmail.com wrote:
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} Dear Arunas,
} You probably already considered this, but whatever you see on TEM is not going to be representative of what's happening in solution, irrespective of preparation method. (Except, maybe freezing/Cryo_TEM?). If what you want to know is how they are dispersed in solution, then DLS or a similar light scattering technique might be best. 20nm particles are well within the size range that's possible. Although, I suppose there will be relatively little contrast due to the material, it should be feasible. In any case, it's a very quick method to try.
} Good luck,
} Pete.
} ____________________________________________________________________________
} Atomic Force Microscopy
} Dr Peter Eaton and Dr Paul West
} Available through all good bookshops, or direct from Oxford University Press at:
} http://ukcatalogue.oup.com/product/9780199570454.do http://afmhelp.com
}
}
} ________________________________________
} X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
} Sent: 21 February 2020 22:25
} To: petereaton-at-hotmail.com
} Subject: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM sample preparation
}
}
}
}
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} Email: arunas.mesceriakovas-at-uef.fi Name: Arûnas Meðèeriakovas
}
} Organization: University of Eastern Finland
}
} Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM
} sample preparation
}
} Message: Hello,
}
}
} I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use
} TEM to check the morphology/size distribution of the sample i.e.
} individual particles or agglomerates. I have prepared the samples by
} dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.
}
} I am aware that due to drying of the solvent from the TEM grid, the
} carbon dots that are present in the drop are forced together and may
} form agglomerates.
} I would like to eliminate/minimize the agglomerate formation in the
} sample preparation process as it is influencing my analysis which is
} used to evaluate the synthesis method (formation of single
} particles/formation of agglomerates).
}
} What are the possible methods I could use in my sample prep, to get the
} least possible particle agglomeration?
}
} Login Host: 193.167.228.180
} Listserver Email Form V - 20120416
} ---------------------------------------------------------------------------
}
}
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} 23, 77 -- "microscopy-at-microscopy.com" {microscopy-at-microscopy.com}
} 23, 77 -- Subject: Re: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM
} 23, 77 -- sample preparation
} 23, 77 -- Thread-Topic: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM
} 23, 77 -- sample preparation
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3, 45 -- Subject: Re: [Microscopy] Re: viaWWW:Avoiding nanoparticle agglomeration in
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From: microscopy.listserver-at-gmail.com
Date: Thu, 27 Feb 2020 00:41:53 -0600
Subject: [Microscopy] viaWWW:EM Lab Manager position at CVM Virginia Tech

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X-from: hancocksk-at-vt.edu

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Email: hancocksk-at-vt.edu

Name: Sandy Hancock

Organization: College of Veterinary Medicine, Virginia Tech

Title-Subject: [Filtered] EM Lab Manager position at CVM Virginia Tech

Message: Hello EM Colleagues,

The Electron Microscopy Laboratory at the College of Veterinary Medicine at Virginia Tech has a
position open for a Lab Manager. The position is responsible for the day-to-day operations of the
service laboratory, which primarily supports researchers on campus who submit biological samples,
with the occasional nanoparticle/materials sample. The laboratory houses a JEOL JEM-1400 TEM, Zeiss
EVO 40 SEM and supporting equipment for specimen preparation. More information can be found at the
position listing http://careers.pageuppeople.com/968/cw/en-us/job/512843/electron-microscopy-lab-mgr

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From: microscopy.listserver-at-gmail.com
Date: Thu, 27 Feb 2020 15:36:13 -0600
Subject: [Microscopy] viaWWW:Buffers other than PBS to use for IEM

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Email: ralvaradojr-at-ufl.edu
Name: Rudy
Organization: University of Florida

Title-Subject: [Filtered] Buffers other than PBS to use for IEM

Message: Hi everyone,
I am trying to process a strain of Lactobacillus johnsonii bacterial cells for immunoelectron
microscopy. These cells hate PBS. My question is: is there other buffers I can use to process this
sample that don't interfere with antigenicity?
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From: microscopy.listserver-at-gmail.com
Date: Thu, 27 Feb 2020 18:32:12 -0600
Subject: [Microscopy] Fwd: Re: viaWWW:Buffers other than PBS to use for IEM

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Try Normal Saline

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Name: Rudy
Organization: University of Florida

Title-Subject: [Filtered] Buffers other than PBS to use for IEM

Message: Hi everyone,
I am trying to process a strain of Lactobacillus johnsonii bacterial
cells for immunoelectron microscopy. These cells hate PBS. My question
is: is there other buffers I can use to process this sample that don't
interfere with antigenicity?
Thanks
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From: Geoffrey McAuliffe :      mcauliff-at-rwjms.rutgers.edu
Date: Thu, 27 Feb 2020 18:32:12 -0600
Subject: Re: [Microscopy] viaWWW:Buffers other than PBS to use for IEM

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PIPES or HEPES

Geoff

**********************************************
Geoff McAuliffe, Ph.D.
Assistant Professor
Director, Electron Microscopy Core
Department of Neuroscience and Cell Biology
Rutgers, Robert Wood Johnson Medical School
675 Hoes Lane West, Piscataway, NJ 08854
voice: (732) 235-4583
mcauliff-at-rwjms.rutgers.edu
**********************************************

==============================Original Headers==============================

==============================End of - Headers==============================






From: Peter van de Plas :      p.vandeplas-at-aurion.nl
Date: Mon, 2 Mar 2020 13:55:40 +0000
Subject: Re: [Microscopy] viaWWW:Buffers other than PBS to use for IEM

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Dear Rudy,

I suggest to process in a buffer that is compatible with your bacterial strain.
Buffers in general will not have a negative effect on antigenicity. I mainly used 100mM PB and PHEM
buffers in the past, when processing cells in culture.

Are you going to chemically fix your specimen with aldehydes?
Do you intend to embed in a plastic?
Choose/work out a fixation method and embedding method compatible with the characteristics of the
primary antibody.
You may test the effect of fixation on signal intensity already on the LM level using an
immunofluorescent method or combine immunogold labeling with silver enhancement.

During the actual immuno incubation it will be beneficial to use a buffer with sodium chloride.
In general PBS, pH 7.4 is a good option. TBS is an alternative. The buffer capacity of Tris at pH
7.4 is however suboptimal. You may go up to a higher pH, e.g., pH 8.2 depending on the additional
additives you use to diminish background staining.
I recommend
https://aurion.nl/sharing-our-knowledge/newsletters-and-newsflyers/newsletter-1-background-suppression/for
additional information.

Kind regards,

Peter van de Plas





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} Title-Subject: [Filtered] Buffers other than PBS to use for IEM
}
} Message: Hi everyone,
} I am trying to process a strain of Lactobacillus johnsonii bacterial
} cells for immunoelectron microscopy. These cells hate PBS. My question
} is: is there other buffers I can use to process this sample that don't
} interfere with antigenicity?
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Peter van de Plas
Aurion
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Wageningen
6709 PD The Netherlands
Phone: +31 317 415094
http://www.aurion.nl







From: microscopy.listserver-at-gmail.com
Date: Thu, 5 Mar 2020 18:47:20 -0600
Subject: [Microscopy] viaWWW:Printing tiff images

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Name: Rick Van Camp

Organization: Kettering University

Title-Subject: [Filtered] Printing tiff images

Message: I would like to display some of the tiff images that have been collected with our TEM in
the hall outside the lab. Can anyone advise me on what properties a given printer needs to possess
such that these images retain the high quality they inherently possess? The idea is to leave a
positive impression on those people that walk by the lab.

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From: wij.muss-at-aon.at
Date: Thu, 5 Mar 2020 19:19:40 -0600
Subject: [Microscopy] Re: Printing tiff images

Contents Retrieved from Microscopy Listserver Archives
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Dear Rick,
Unfortunately I don’t have practical experience with PINTING TIFF files properly…don’t own a
TIFF-printable printer (☺)


But: Information is available:
e.g. https://itstillworks.com/print-large-tiff-file-6469452.html

Copy & Paste: Just for convenience of all Readers/colleagues eventually not being able to access the
given URL:
QUOTE: How to Print a Large Tiff File
Step 1
Select a paper that is suited to print photographic images. Using plain typing paper or copy paper
will not capture the range of values and colors inherent in a high-quality Tiff file. If possible,
select the same type of paper as the manufacturer of your printer.
Step 2
Access a printer that is designed to Tiff quality images, if possible. Most home inkjet printers
will print a photographic image of an acceptable quality, though photo-specific printers will give
the contrast and color range that a Tiff is designed to provide. These types of printers often have
the word "photo" in their model name, and use individual-color ink cartridges.
Step 3
Clean the print heads on your printer. This prevents lines, known as banding, as well as color
shifts. Right-click on the printer icon in the taskbar, located on the lower right corner of your
screen. Follow the step-by-step instructions after clicking the option to clean the print heads.
Step 4
Open the Tiff file in your computer's imaging software or default picture viewer. Make any changes
to contrast, color and cropping as necessary or possible.
Step 5
Select Print from the File menu in the software. Select the option called Page Options or Print
Options, depending on your software model. This will bring up a dialog box specific to your printer.
Step 6
Choose the paper size, orientation, the source. The size will likely be legal, or 8.5 by 11 inches.
The orientation refers to whether the image is tall (portrait) or long (landscape). The source is
either a sheet or roll of paper.
Step 7
Select the type of media being used. The instruction sheet with your paper may suggest a certain
media based on your printer manufacturer. There may be a number of options to experiment with, but
the important detail is to select whether the paper you have chosen has a glossy surface or is matte.
Step 8
Select the highest print quality available. This option is known under various terms depending on
the manufacturer, but will usually be the top or last quality in a list of options.
Load the paper into the printer on its correct side as explained by the paper's instruction sheet.
Press print. If the print has problems with its contrast or color, return to the file in the imaging
software. Make the necessary changes and repeat the process as needed.
Tip
• So as not to waste ink, use the selector tool in your imaging software to choose a portion of
the image with a variety of colors or contrast. Copy and paste this portion into a new document.
Make the necessary changes and print test strips until it is determined what changes to apply to the
entire image. Items you will need
• Computer with imaging software or default picture viewer
• Tiff file
• Printer, preferably of photographic quality

Elizabeth MOTT,
My Printer Is Not Printing TIFF Files
https://smallbusiness.chron.com/printer-not-printing-tiff-files-57302.html
[ Related Articles,
References (3)
https://lbis.kenyon.edu/helpline/printers/troubleshooting
Microsoft: Description of the Guidelines for Selecting the Appropriate Picture Format in an
Office Program
Adobe Systems: Developer Resources: TIFF
Resources (1)
Dux Computer Digest: How to Troubleshoot Printer Problems ]
What Is the Best Image Format for Printing?
https://www.shutterstock.com/support/article/best-image-format-for-printing

Asked Feb 2019:
https://photo.stackexchange.com/questions/104804/jpeg-or-tiff-files-better-for-print-images
JPEG or TIFF files better for print images?

Asked August 2007
https://www.photo.net/discuss/threads/is-it-possible-to-print-tiff-files.283228/

Best regards and good luck,

Wolfgang MUSS
SALZBURG, AUSTRIA

=========================================================
MUSS Wolfgang Dr. phil. / PhD - retired
Ignaz-Rieder-Kai 19/6
A-5020 SALZBURG
Österreich-AUSTRIA
Mobile-Tel.: 0043(0)676 5 369 456
E-mail: wij.muss-at-aon.at
E-Mail altern.: womuss-at-gmail.com
FRMS, Retired Member of MSA & other (Inter-)National Societies
Former Head of Electron Microscopy Lab at Institute of Pathology
SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL
UNIVERSITY of SALZBURG
Scientific Profile at ResearchGate:
http://www.researchgate.net/profile/Wolfgang_MUSS
inviting you to join RG (Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html,
and join 15+ million researchers, including 63 Nobel Laureates)
„ResearchGate is the professional network for scientists and researchers. Over 15 million members
from all over the world use it to share, discover, and discuss research. We're guided by our mission
to connect the world of science and make research open to all.“

===================================================================================
Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Freitag, 6.
März 2020 01:48
An: wij.muss-at-aon.at
Betreff: [Microscopy] Printing tiff images

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From: julietran.tsuki-at-gmail.com
Date: Thu, 5 Mar 2020 18:02:45 -0800
Subject: Re: [Microscopy] viaWWW:Printing tiff images

Contents Retrieved from Microscopy Listserver Archives
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Hello Rick

If you are interested in printing out your images with your printer. Make sure that your printer has
the capabilities to print on photo paper and what type of ink it uses. If you check the name/version
of your printer you can look up to see what your printer can and cannot do for printing qualities.
When you do print be sure to make sure that the file format/ size of image you have. Due to when you
print out your image and you manipulate it may warp the image.  Here are some links to help you.
-Julie

Link best printer for photos
} }
https://www.bestproducts.com/tech/electronics/a26933257/top-rated-photo-printers/?src=arb_ga_bp_m_bm_a26933257&utm_source=google&utm_medium=cpc&utm_campaign=arb_ga_bp_m_bm_a26933257&gclid=EAIaIQobChMIgPyip-CE6AIVkspkCh1Mowy8EAAYASAAEgJGa_D_BwE

Why do pictures look different when I print them link
} }
https://www.google.com/amp/s/www.howtogeek.com/397798/why-do-photos-look-different-when-i-print-them/amp/

Sent from my iPhone

} On Mar 5, 2020, at 3:51 PM, microscopy.listserver-at-gmail.com wrote:
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} Title-Subject: [Filtered] Printing tiff images
}
} Message: I would like to display some of the tiff images that have been collected with our TEM in
} the hall outside the lab.  Can anyone advise me on what properties a given printer needs to possess
} such that these images retain the high quality they inherently possess?  The idea is to leave a
} positive impression on those people that walk by the lab.
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From: oshel1pe-at-cmich.edu
Date: Fri, 6 Mar 2020 08:26:07 -0600
Subject: [Microscopy] Re: viaWWW:Printing tiff images

Contents Retrieved from Microscopy Listserver Archives
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Rick,

1) Epson Photo printer with Photo inks, 6 to 9 color (the blue ink is used in B&W printing). But!
Don't buy one unless you will be printing at least once a month, maybe even once a week. Inks are
hideously expensive and 3rd party inks don't work well or at all (inks are bar coded so a printer
company can prevent other brands or refills from being used). However. Unless you do print
something, even a test page, every so often, the ink dries, clogs a valve or three, and this cannot
be fixed. The printer is trash.
So, if you just want a set of images for the hallway, use the campus printing service or a business.

2) High quality photo paper. Let the ink completely dry (it sits on top of the coating, hence higher
resolution printing) for 30-60 minutes. *Laminate* the print. This seals out oxygen, which fades images.

3) When moving the image files, do *not* use drag-and-drop, use copy/paste. Drag and drop in Windows
can change the image resolution to the monitor's resolution. This was true, I do not know if it is
still true with Windows 10 (or 7), but I don't trust it. I don't know about Linux, and I don't think
MacOS does this.
But copy/paste is easy.

4) Not what you asked, but more of a tech-looking thing that makes admin types happy: Put up a
display monitor or three in the hallway, and set up a computer to send images to the monitors. No
printer, no buying expensive inks, no worrying about file types and sizes, easier to change images,
etc. You can maybe get the monitors and computer from campus IT - ones that have been replaced with
something newer, but are still perfectly good and likely free.

Phil
------------- Philip Oshel Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office

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Email: rvancamp-at-kettering.edu
Name: Rick Van Camp
Organization: Kettering University
Title-Subject: [Filtered] Printing tiff images
Message: I would like to display some of the tiff images that have been collected with our
TEM in the hall outside the lab. Can anyone advise me on what properties a given printer needs
to possess such that these images retain the high quality they inherently possess? The idea is
to leave a positive impression on those people that walk by the lab.


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From: microscopy.listserver-at-gmail.com
Date: Sat, 7 Mar 2020 12:45:41 -0600
Subject: [Microscopy] Fwd: Microscopy-microanalysis technician position

Contents Retrieved from Microscopy Listserver Archives
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X-from: Harland, Duane {Duane.Harland-at-agresearch.co.nz}


Dear colleagues,

We have a job vacancy for a permanent position, part time, technician in our
microscopy/microanalysis laboratory here at AgResearch Institute in New Zealand.

We are primarily working on biological and bio-based materials using SEM, TEM and other similar
methods. We are looking to hire a technician to carry out a range of tasks from assisting with
sample preparation to helping look after the instrumentation and do some troubleshooting etc. The
role would suit someone with a good working knowledge with instrumentation, an eye for detail and
good collaboration skills.

For more details look under AgResearch on the Science New Zealand website under careers for more
details.

https://careers.sciencenewzealand.org/agresearch-jobdetails/ajid/xcR09/Microscopy-and-Microanalysis-Technician,37409.html

Duane

*AgResearch Limited | *Lincoln Research Centre *| New Zealand*
Dr Duane P Harland, Senior Scientist
*T*+64 3 321 8710 *E*duane.harland-at-agresearch.co.nz {mailto:duane.harland-at-agresearch.co.nz}

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From: microscopy.listserver-at-gmail.com
Date: Sat, 7 Mar 2020 12:48:16 -0600
Subject: [Microscopy] viaWWW:We are hiring! Electron Microscopist / Imaging Specialist

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X-from: mrajasingham-at-sivananthanlabs.us

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Email: mrajasingham-at-sivananthanlabs.us Name: Madhura Rajasingham

Organization: Sivananthan Laboratories, Inc.

Title-Subject: [Filtered] We are hiring! Electron Microscopist / Imaging Specialist

Message: Hello!
Sivananthan Laboratories, Inc. is a high-tech business incubator focused on promoting economic
growth in Illinois and the United States through fostering cutting-edge, fundamental research and
development. We work on R&D for AI, infrared detectors, image processing, solar energy, and more.
We are currently looking for a successful candidate who is skilled in all aspects on scanning
electron microscopy (SEM), transmission electron microscopy (TEM) and scanning transmission electron
microscopy (STEM) with experience in image simulation and state-of-the-art processing and analytics
packages. If you are interested, please go to this link (http://sivananthanlabs.us/employment/) and
apply for this position!

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From: microscopy.listserver-at-gmail.com
Date: Sat, 7 Mar 2020 14:52:56 -0600
Subject: [Microscopy] Fwd: LaB6 on FEI BioTwin12

Contents Retrieved from Microscopy Listserver Archives
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X-from: Ning, Gang {gxn7-at-psu.edu}

Dear Listers,

One of my users wants to replace a tungsten filament with aLaB6tip in my FEI BioTwin12 for
screening/imaging cryoEM samples. I know aLaB6is much more expensive. But regardless the cost, what
are other downside impact on my other users who primarily use the scope for imaging plastic
sections, negative-stained samples? Thank you!

Best regards,

Greg

Gang (Greg) Ning

Microscopy Facility

Huck Institutes of the Life Sciences

Penn State University

MSC N-048

University Park, PA 16802

814-863-0994

https://www.huck.psu.edu/core-facilities/microscopy-facility



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From: Erico Freitas :      ericotadeu-at-ufmg.br
Date: Sat, 7 Mar 2020 18:27:00 -0300
Subject: Re: [Microscopy] Fwd: LaB6 on FEI BioTwin12

Contents Retrieved from Microscopy Listserver Archives
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Hi Greg,


We're about to the same in our Tecnai Spirit Biotwin T12. The FEI/Thermofisher guys have no concern
about that.

I've used a T12 in CMM at the University of Queensland, Brisbane/Australia that has got a LaB6.

You must clean the gun assembly and the Wehnelt as well in order to get rid of W residues before.

Although a LaB6 is more expensive than a W filament, its life time is much longer. It might last
over 1000 ours. It lasts 2000 hours in average on our Tecnai T20. You know it will give you a
brighter beam, and its energy spread is lower.

Regarding the other users, they will be quite happy when looking at their plastic sections and
negative staining, not only the cryo-EM users.

It's always a good practice to set a lower C2 aperture and play around with the spot size to a
suitable electron dose for each sample. I know some users don't like to change the apertures and do
the alignments but they should get use of it.

My only concern is about the gun preassure. As the Tecnai Spirit Biotwin T12 does have a dedicated
IGP for the gun (ours doesn't have), the vacuum in the gun is broken down every time you run the
cryo cycle. So the LaB6 cathode gets contaminated. But I still think the benefits of the LaB6 will
give you are worth to have it on your T12.


Best wishes,


On Sat, 7 Mar 2020, 16:56 , {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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X-from:         Ning, Gang {gxn7-at-psu.edu {mailto:gxn7-at-psu.edu} }

Dear Listers,

One of my users wants to replace a tungsten filament with aLaB6tip in my FEI BioTwin12 for
screening/imaging cryoEM samples. I know aLaB6is much more expensive. But regardless the cost, what
are other downside impact on my other users who primarily use the scope for imaging plastic
sections, negative-stained samples?    Thank you!

Best regards,

Greg

Gang (Greg) Ning

Microscopy Facility

Huck Institutes of the Life Sciences

Penn State University

MSC N-048

University Park, PA 16802

814-863-0994

https://www.huck.psu.edu/core-facilities/microscopy-facility




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From: microscopy.listserver-at-gmail.com
Date: Sat, 7 Mar 2020 16:20:25 -0600
Subject: [Microscopy] Re: Fwd: LaB6 on FEI BioTwin12

Contents Retrieved from Microscopy Listserver Archives
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Hello Greg,

if your FEI TEM is equipped with a getter pump and does reach a sufficient vacuum value you will
generally benefit from switching to LaB6 cathodes. An LaB6 cathode can be pre-mounted in a spare
wehnelt and interchanged within an hour, if the vacuum system in the rest of the scope is pumped
down to limit. Use dry Nitrogen gas for flooding and let it run until cathode / wehnelt is
changed... LaB6 cathode needs to be centred VERY well, within a few 1/100 mm. For accurate height
settings see the data sheets at Kimball or DENKA websites.

The spot diameter will be smaller than with Tungsten, the electrons coming from the cathode more
monochromatic. Both things will help getting better resolution / better focus and astigmatism
corrections in the images.

The luminosity of the gun will be significantly higher; that would help at magnifications more than
50-100 k... But: very thin UM cuts could burn under a concentrated beam.

I suppose the automatic heating regime for the LaB6 cathode could be selected at the TEM setup. It
will need slow heating (and cooling), ca. 2 minutes each.

Other than the cathode handling and the costs (but: cathode will run ca. 1000-2000 hours) there is
only win-win...

I use LaB6 since a lot of years at a Philips / FEI EM420.


Best,

Stefan


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
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Websites:
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Am 07.03.20 um 22:00 schrieb microscopy.listserver-at-gmail.com:
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} X-from: Ning, Gang {gxn7-at-psu.edu}
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} Dear Listers,
}
} One of my users wants to replace a tungsten filament with aLaB6tip in my FEI BioTwin12 for
} screening/imaging cryoEM samples. I know aLaB6is much more expensive. But regardless the cost, what
} are other downside impact on my other users who primarily use the scope for imaging plastic
} sections, negative-stained samples? Thank you!
}
} Best regards,
}
} Greg
}
} Gang (Greg) Ning
}
} Microscopy Facility
}
} Huck Institutes of the Life Sciences
}
} Penn State University
}
} MSC N-048
}
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From: microscopy.listserver-at-gmail.com
Date: Sun, 8 Mar 2020 16:39:49 -0500
Subject: [Microscopy] Administrivia: Sorry all this is just a system test - Your Friendly

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From: microscopy.listserver-at-gmail.com
Date: Mon, 9 Mar 2020 15:01:55 -0500
Subject: [Microscopy] viaWWW: lead aspartate as post-stain

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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] lead aspartate as post-stain

Message: Hello everyone,
I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate).
Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep.
If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate
any and all information.

THanks!
John Shields
Univ. of Georgia

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From: microscopy.listserver-at-gmail.com
Date: Mon, 9 Mar 2020 18:12:53 -0500
Subject: [Microscopy] viaWWW: lead aspartate as post-stain

Contents Retrieved from Microscopy Listserver Archives
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

John,

Lead aspartate? Isn't that used as a sweetner in some of the heavier diet pastries?

Phil
-----------------------------------------
Philip Oshel
Imaging Center Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576 office
(989) 774-7567 lab



________________________________________
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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] lead aspartate as post-stain

Message: Hello everyone,
I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate).
Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep.
If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate
any and all information.

THanks!
John Shields
Univ. of Georgia

Login Host: 198.137.20.67
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From: microscopy.listserver-at-gmail.com
Date: Wed, 11 Mar 2020 09:01:26 -0500
Subject: [Microscopy] viaWWW: lead aspartate as post-stain

Contents Retrieved from Microscopy Listserver Archives
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X-from: Frank Karl {frank_karl-at-ardl.com}


No no no, you're think of sugar of lead or lead acetate. Hey, it worked for the Roman Empire.




Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, March
9, 2020 7:19 PM
To: Frank Karl {frank_karl-at-ardl.com}

X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

John,

Lead aspartate? Isn't that used as a sweetner in some of the heavier diet pastries?

Phil
-----------------------------------------
Philip Oshel
Imaging Center Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576 office
(989) 774-7567 lab



________________________________________
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Sent: Monday, March 9, 2020 16:08
To: Oshel, Philip Eugene

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Email: jpshield-at-uga.edu Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] lead aspartate as post-stain

Message: Hello everyone,
I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate).
Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep.
If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate
any and all information.

THanks!
John Shields
Univ. of Georgia

Login Host: 198.137.20.67
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From: John Shields :      johnshields59-at-gmail.com
Date: Wed, 11 Mar 2020 15:42:25 -0400
Subject: Re: [Microscopy] Fwd: RE: Fwd: Re: viaWWW: lead aspartate as post-stain

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Oh Right, right,
Sorry,  I mean the "Stain formerly known as Lead Aspartame".
Sheesh
John S

On Wed, Mar 11, 2020 at 9:04 AM {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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X-from: Frank Karl {frank_karl-at-ardl.com {mailto:frank_karl-at-ardl.com} }


No no no,  you're think of sugar of lead or lead acetate.   Hey, it worked for the Roman Empire.




Stay safe...........

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio  44305

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Subject: [Microscopy] Fwd: Re: viaWWW: lead aspartate as post-stain




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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }

John,

Lead aspartate? Isn't that used as a sweetner in some of the heavier diet pastries?

Phil
-----------------------------------------
Philip Oshel
Imaging Center Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576 office
(989) 774-7567 lab



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Subject: [Microscopy] viaWWW: lead aspartate as post-stain




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Email: jpshield-at-uga.edu {mailto:jpshield-at-uga.edu} Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] lead aspartate as post-stain

Message: Hello everyone,
I have been searching for any work on lead aspartate used as a post-stain (similar to lead
citrate).
Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample
prep.
If anyone has literature, or has done some previous work seeing if LA would work, I would
appreciate
any and all information.

THanks!
John Shields
Univ. of Georgia

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From: microscopy.listserver-at-gmail.com
Date: Thu, 12 Mar 2020 09:17:16 -0500
Subject: [Microscopy] viaWWW: High Vacuum STM available

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Email: mbrukman-at-seas.upenn.edu Name: Matthew Brukman

Organization: University of Pennsylvania

Title-Subject: [Filtered] High Vacuum STM available

Message: Good morning!

We have a surplus high vacuum STM system free to a good home. Components include

RHK controller
Omicron STM-1 head
vacuum chamber
PC with RHK software
roughing, turbo, and ion pumps
frame with air-supported legs

Recipient would be responsible for transport and reassembly. Please contact me with questions.

Regards,
Matt


Matthew Brukman
Scanning and Local Probe Facilities Director
Singh Center for Nanotechnology
3205 Walnut St. Phila., PA 19104
215-746-2373

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From: Alan.Paris-at-leica-microsystems.com
Date: Fri, 13 Mar 2020 08:40:56 -0500
Subject: [Microscopy] LM: Recommended Materials/Procedure for Disinfecting Optical

Contents Retrieved from Microscopy Listserver Archives
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Is there a recommended procedure /materials for disinfecting optical microscope eyepieces, optics
and surfaces against COVID-19?

Alan Paris
Leica Microsystems Inc.
alan.paris-at-leica-microsystems.com









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From: microscopy.listserver-at-gmail.com
Date: Mon, 16 Mar 2020 17:34:03 -0500
Subject: [Microscopy] viaWWW: ] How to clean nickel shim of magnetic and or glass particles

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Email: nmz787-at-gmail.com Name: Nathan McCorkle

Organization: Sole proprietor

Title-Subject: [Filtered] How to clean nickel shim of magnetic and or glass particles?

Message: I have a nickel shim destined for nanoimprint lithography, made by electroforming e-beam
exposed photoresist. I don't have a proper cleanroom, but I've been trying to strip what seemed like
residual ZEP e-beam resist... And it has not been going so well. I've tried acetone,
dichloromethane, n-methyl pyrrolidone, and 10% NaOH. Sonicated with heat in both acetone and NaOH
(at different times). The NaOH is the most recent attempt, and it seemed to show improvement under
FIB imaging, but I also noticed what appeared to be redeposition. I can only imagine this is due to
particulate in my solvents, dirty air as I blow dry the shim or carry it from my sonicator to my FIB
desk, or maybe insoluble particles like glass or ferromagnetic dust which start to settle onto the
sample as soon as the sonicator is turned off.

Features are around 150nm linewidth, high frequency and complex shaped... So lots of small say 500nm
sized holes/crevices which I'd been thinking was just diffusion limited for the solvent to get into
and do it's work. But now I'm pretty confused.

Should I invest in some .45 and .22 micron syringe filters for all my fluid work? Should I tape a
magnet to the outside of the beaker I've been sonicating in, to try and collect such particles?
What's a standard semiconductor lab method for cleaning magnetic particles from magnetic layers? How
about the idea of insolubles? Or can someone recommend a solution that will etch glass but not nickel?

Thanks,
-Nathan

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From: mabon-at-illinois.edu
Date: Tue, 17 Mar 2020 13:07:41 -0500
Subject: [Microscopy] Job Opening: University of Illinois at Urbana-Champaign seeking a

Contents Retrieved from Microscopy Listserver Archives
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Research Scientist Materials Research Laboratory
University of Illinois at Urbana-Champaign

The Materials Research Laboratory (MRL) at the University of Illinois at Urbana-Champaign is seeking
a highly motivated Research Scientist to participate and actively support research in the area of
scanning electron microscopy. The Research Scientist will provide comprehensive technical support
and user training for equipment, procedures, and safety in the broad areas of materials
microanalysis and microfabrication.

The successful candidate will become a member of the dedicated staff of approximately 20 scientists
and engineers, who maintain major research instruments in the MRL's core facilities for electron
microscopy, soft materials characterization, scanning probe microscopy, laser and optical
spectroscopy, surface analysis, x-ray diffraction, and nanofabrication facilities including two
cleanrooms. Approximately 1,000 researchers from across our campus as well as other academic
institutions, industry, and national laboratories use the facilities, logging more than 100,000 user
hours annually. The lab is recognized as one of the premier mid-sized user facilities in the nation.
For details, please visit us online at https://mrl.illinois.edu/facilities/.

The University of Illinois is an Equal Opportunity, Affirmative Action employer. Minorities, women,
veterans and individuals with disabilities are encouraged to apply. For more information, visit
http://go.illinois.edu/EEO.

Responsibilities include:
* Prepare and deliver primary and advanced training on the various techniques and instrumentation
available in the electron microscopy core, particularly in scanning electron microscopy and related
techniques.
* Formulate, compile and distribute suitable suggestions for documentation improvements for
collective staff scientist review. Incorporate approved modifications into training documents and
procedures. This includes the creation of video and multimedia tutorials.
* Actively participate in research using electron microscopy techniques in new materials, such as
ceramics, metals, semiconductor multilayers and super lattices, polymer and biological materials, etc.
* Perform routine preventative maintenance tasks which vary daily, monthly, and annually for
assigned laboratory instrumentation. Examples include daily checks to ensure instruments are running
correctly; monthly proactive checks to ensure no hazards are present; annual maintenance service on
vacuum pumps and supporting mechanical equipment.
* Identify hazards and/or potential failure modes by comparing equipment usage and performance to
establish safety protocols while conducting user training or performing maintenance. If the
equipment is not operating within tolerances or any engineering safety controls are malfunctioning,
determine corrective actions to hardware, operating procedures or user training in conjunction with
the assigned staff scientist and implement the changes.
* Crosstrain in other facility techniques in order to act as backup for other staff members and
increase knowledge and experience.
* Give oral presentations to large audiences, including recording training material for media
outlets, and online, live video streaming.
* Perform facility-wide safety tasks as assigned.
* Conduct department or campus specified lab inspections for assigned operating areas and
participate in reviews of non-assigned areas as requested.
* Assume additional responsibilities to promote the unit's mission as needed.

Minimum Qualifications
* Ph.D. degree in engineering, chemical, physical sciences or related field.
* Two years of hands-on experience in the following areas:
* operation of scanning electron microscopes, including detailed knowledge of main physical
principles, concepts and applications of electron microscopy;
* training researchers in the use of scanning electron microscopes including data interpretation on
related techniques;
* troubleshooting, preventive maintenance and routine repair of scanning electron microscopes;
* use of advanced analytical techniques such as energy-dispersive X-ray spectrometry,
cathodoluminescence imaging and spectrometry, and electron backscatter diffraction using the
scanning electron microscope.
* sample preparation techniques for electron microscopy including polishing, coating, milling, etc.
* Three years of instructional/training experience delivering technical information.
* Previous experience in creating/developing instructional, instrument operation and training
material in electron microscopy.
* Excellent oral and written communication skills.

Preferred Qualifications
* Experience working in a multi-user academic research facility.
* Post-doctoral experience in engineering, chemical, physical sciences or related field.
* Chemistry background necessary for identifying chemicals for waste processing.
* Experience with operation of focused ion beam (FIB/SEM) systems and transmission electron
microscopes and related techniques.

This is a full-time, benefits-eligible academic professional position appointed on a 12-month
service basis. The expected start date is as soon as possible. Salary is commensurate with
experience and qualifications. To apply, please complete a candidate profile at
http://jobs.illinois.edu and upload the following as a single file: a cover letter, resume, and the
names and contact information for three professional references. To ensure full consideration, all
requested information must be submitted by April 2, 2020.

For further information regarding application procedures, contact Summer Redman at
mailto:sredman-at-illinois.edu or 217-300-5400.

The University of Illinois conducts criminal background checks on all job candidates upon acceptance
of a contingent offer. As a qualifying federal contractor, the University of Illinois System uses
E-Verify to verify employment eligibility. The University of Illinois must also comply with
applicable federal export control laws and regulations and, as such, reserves the right to employ
restricted party screening procedures for applicants.


________________________________________
James C Mabon, Ph.D.
Senior Research Scientist
University of Illinois at Urbana-Champaign
Materials Research Laboratory
104 S. Goodwin Ave.
Urbana, Illinois, 61801
________________________________________

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From: microscopy.listserver-at-gmail.com
Date: Sat, 21 Mar 2020 08:42:56 -0500
Subject: [Microscopy] viaWWW: Teaching SEM Online

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Email: Stephen.Beck-at-ncc.edu Name: Steve Beck

Organization: Nassau Community College

Title-Subject: [Filtered] Teaching SEM Online

Message: Dear Colleagues,
I am teaching my SEM course this semester and like many institutions, we are trying to teach
online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online,
however, after that we need to get on the SEM to image the required samples.
Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
this is impossible - I haven't received the courtesy of a response yet!

Thanks!
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From: microscopy.listserver-at-gmail.com
Date: Sat, 21 Mar 2020 09:51:42 -0400
Subject: Re: [Microscopy] viaWWW: Teaching SEM Online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: lolita.rotkina-at-yandex.com {lolita.rotkina-at-yandex.com}
Envelope-From: lolita-rotkina-at-yandex.ru


Hello Steve!

It is very challenging situation, but everything depends on what generation of hardware you have in
the lab.
Depends on who is your vendor, it may be possible to share the screens and allow users to move the
mouse and type on the screen.
Such things exist for the troubleshooting on most of the modern instruments. Technically, some
instruments can be even used remotely for a high quality work. The only bottleneck I'd - someone
needs to.prepare the sample and mount it inside the analytical tool.
As the instruments get more and more sophisticated and  automated - that approach may become more
popular anyway.

Ask your vendor - they may already have the answer for you.

Stay healthy!

Lolita
8:45 AM, March 21, 2020, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} :




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Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck

Organization: Nassau Community College

Title-Subject: [Filtered] Teaching SEM Online

Message: Dear Colleagues,
I am teaching my SEM course this semester and like many institutions, we are trying to teach
online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam
online,
however, after that we need to get on the SEM to image the required samples.
Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
this is impossible - I haven't received the courtesy of a response yet!

Thanks!
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Lolita Rotkina, PhD

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From: David Huskisson :      david-at-emanalytical.co.uk
Date: Sat, 21 Mar 2020 14:07:31 +0000
Subject: Re: [Microscopy] viaWWW: Teaching SEM Online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi Stephen,

We have a couple of our SEMs connected to the internet for remote access. Through this interface,
the microscope is completely controllable from your own PC. We can load your samples and then you or
your students would be able to take control of the instrument and practice from your own homes.

Let me know how we can help.

Kind regards,

/David Huskisson, PhD./
Project Scientist & Microscopist


Alderley Park
Macclesfield
SK10 4TG

Office: +44 (0) 1625 704 467
DD:     +44 (0) 1625 238 869
Mob:  +44 (0) 7837 718 098

www.emanalytical.co.uk {http://www.emanalytical.co.uk/}


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On Sat, Mar 21, 2020 at 1:48 PM {microscopy.listserver-at-gmail.com
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Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck

Organization: Nassau Community College

Title-Subject: [Filtered] Teaching SEM Online

Message: Dear Colleagues,
I am teaching my SEM course this semester and like many institutions, we are trying to teach
online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam
online,
however, after that we need to get on the SEM to image the required samples.
Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
this is impossible - I haven't received the courtesy of a response yet!

Thanks!
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From: diller-at-stefan-diller.com
Date: Sat, 21 Mar 2020 13:00:11 -0500
Subject: [Microscopy] Re: viaWWW: How to clean nickel shim of magnetic and or

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Nathan,

did you try using a plasma cleaner for cleaning the surfaces and also a plasma cleaner like the
Evactron at the FIB chamber to keep the specimen clean during scanning?

If you can mount the specimen with the surface to be cleaned facing down to the bottom of the beaker
you might get rid of deposits coming from above ;-)

Another try to clean the surfaces might be to plunge it in liquid nitrogen or to use a vacuum
chamber with the cleaning solution and pump to a level below sublimation.

And sure: clean micro-filtered solutions would help.

Nickel and magnetism: you could use a demagnetizer to decrease / erase the magnetism in the shim first.


Best wishes,

Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 21.03.20 um 14:55 schrieb microscopy.listserver-at-gmail.com:
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} Email: nmz787-at-gmail.com Name: Nathan McCorkle
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} Title-Subject: [Filtered] How to clean nickel shim of magnetic and or glass particles?
}
} Message: I have a nickel shim destined for nanoimprint lithography, made by electroforming e-beam
} exposed photoresist. I don't have a proper cleanroom, but I've been trying to strip what seemed like
} residual ZEP e-beam resist... And it has not been going so well. I've tried acetone,
} dichloromethane, n-methyl pyrrolidone, and 10% NaOH. Sonicated with heat in both acetone and NaOH
} (at different times). The NaOH is the most recent attempt, and it seemed to show improvement under
} FIB imaging, but I also noticed what appeared to be redeposition. I can only imagine this is due to
} particulate in my solvents, dirty air as I blow dry the shim or carry it from my sonicator to my FIB
} desk, or maybe insoluble particles like glass or ferromagnetic dust which start to settle onto the
} sample as soon as the sonicator is turned off.
}
} Features are around 150nm linewidth, high frequency and complex shaped... So lots of small say 500nm
} sized holes/crevices which I'd been thinking was just diffusion limited for the solvent to get into
} and do it's work. But now I'm pretty confused.
}
} Should I invest in some .45 and .22 micron syringe filters for all my fluid work? Should I tape a
} magnet to the outside of the beaker I've been sonicating in, to try and collect such particles?
} What's a standard semiconductor lab method for cleaning magnetic particles from magnetic layers? How
} about the idea of insolubles? Or can someone recommend a solution that will etch glass but not nickel?
}
} Thanks,
} -Nathan
}
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From: diller-at-stefan-diller.com
Date: Sat, 21 Mar 2020 13:14:30 -0500
Subject: [Microscopy] Re: viaWWW: Teaching SEM Online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stephen,

if nobody can help you nearby and as long as Corona will stay outside my lab we can try set
something as a life conference from my lab (I might need some help for this, so it might take some
time to set it up...).

I am using a FE-SEM TESCAN MIRA3 and a Philips / FEI TEM EM420.

Some nice resources can be found by searching at google for "teaching electron microscopy"

and here

https://www.fei.com/education-resources/


For some outreach to your students you can use my nanoflight channel at vimeo to show what is
possible with electron microscopy and a lot of effort.

https://vimeo.com/channels/nanoflight

and if you like I can put some more 3D nanoflights online for the OCULUS or other 3D glasses.



Best wishes,

Stefan


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 21.03.20 um 14:52 schrieb microscopy.listserver-at-gmail.com:
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} I am teaching my SEM course this semester and like many institutions, we are trying to teach
} online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online,
} however, after that we need to get on the SEM to image the required samples.
} Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
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18, 29 -- From diller-at-stefan-diller.com Sat Mar 21 13:14:29 2020
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From: vray-at-partbeamsystech.com
Date: Sat, 21 Mar 2020 18:31:40 -0500
Subject: [Microscopy] Re: viaWWW: Teaching SEM Online

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Nathan,

To clean a surface of particulates, I would use replicating tape. This is a cellulose acetate tape
(non-adhesive) that you soften with acetone and press down onto the surface. Let it dry and peel it
off. All the particulates should come with it. I've had better luck in removing particulates this
way compared with ultrasonics, rinses, etc.

I'm not sure if an adhesive tape will work but if you don't have replicating tape, you might try
some of the tape with the "Post-it" type adhesive. It may take several applications to remove
everything.
Replicating tape is available from most of the EM supply houses. It comes in both a thick and thin
form.
Cheers,
Henk

----------------


Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Saturday, March 21,
2020 9:53 AM
To: Colijn, Hendrik {colijn.1-at-osu.edu}


Hi Stephen,

Depending on the size of your class - you can use one of remote desktop packages, such as Teamviwer,
VNC, RDP, Chrome remote desktop, etc... to let your students see (and even operate) GUI of the SEM,
and hear your explanations.

I've been using TeamViewer for remote troubleshooting and training of FIB operators a couple of
years, although haven't tried it in "classroom" kind of approach.

Best Wishes,
Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 3/21/2020 9:43 AM, microscopy.listserver-at-gmail.com wrote:
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} Email: Stephen.Beck-at-ncc.edu Name: Steve Beck
}
} Organization: Nassau Community College
}
} Title-Subject: [Filtered] Teaching SEM Online
}
} Message: Dear Colleagues,
} I am teaching my SEM course this semester and like many institutions, we are trying to teach
} online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online,
} however, after that we need to get on the SEM to image the required samples.
} Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
} this is impossible - I haven't received the courtesy of a response yet!
}
} Thanks!
} Login Host: 173.77.159.14
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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Apr 2020 07:52:48 -0500
Subject: [Microscopy] Fwd: TEM/AFM Researcher position (191486)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



X-from: David Huskisson {david-at-emanalytical.co.uk}


Hi Stephen,

We have a couple of our SEMs connected to the internet for remote access. Through this interface,
the microscope is completely controllable from your own PC. We can load your samples and then you or
your students would be able to take control of the instrument and practice from your own homes.

Let me know how we can help.

Kind regards,

/David Huskisson, PhD./
Project Scientist & Microscopist


Alderley Park
Macclesfield
SK10 4TG

Office: +44 (0) 1625 704 467
DD:     +44 (0) 1625 238 869
Mob:  +44 (0) 7837 718 098

www.emanalytical.co.uk {http://www.emanalytical.co.uk/}


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On Sat, Mar 21, 2020 at 1:48 PM {microscopy.listserver-at-gmail.com
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Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck

Organization: Nassau Community College

Title-Subject: [Filtered] Teaching SEM Online

Message: Dear Colleagues,
I am teaching my SEM course this semester and like many institutions, we are trying to teach
online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam
online,
however, after that we need to get on the SEM to image the required samples.
Does anyone have any ideas regarding teaching SEM online? I have informed my administration that
this is impossible - I haven't received the courtesy of a response yet!

Thanks!
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From kelnanc57ctyee-at-gmail.com Sat Mar 28 20:49:33 2020
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X-from: Erico Freitas {ericotadeu-at-ufmg.br}



Dear all,


I'm just forwarding you the following TEM/AFM Researcher position (191486)
at CNPEM.

The CNPEM is located in Campinas, S=C3=A3o Paulo state, Brazil.

See more details through this link:

https://pages.cnpem.br/trabalheconosco/2020/03/23/researcher-with-a-focus-o=
n-electron-transmission-microscopy-or-atomic-force-191486/
{https://pages.cnpem.br/trabalheconosco/2020/03/23/researcher-with-a-focus-o=n-electron-transmission-microscopy-or-atomic-force-191486/}


This position is likely for the LNNano (Brazilian National Nanotechnology
Laboratory) at CNPEM (https://lnnano.cnpem.br/)


Cheers,

--
Erico Freitas

Physicist/Microscopist at Center of Microscopy
Universidade Federal de Minas Gerais (UFMG)
Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901.
+55-31-3409-7573
+55-31-3409-7575

Coordinator:Transmission Electron Microscopy laboratory

CV Lattes: *http://lattes.cnpq.br/8786127123101199*
{https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#}

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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Apr 2020 08:17:42 -0500
Subject: [Microscopy] Fwd: TEM/AFM Researcher position (191486)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


X-from: Erico Freitas {ericotadeu-at-ufmg.br}



Dear all,


I'm just forwarding you the following TEM/AFM Researcher position (191486)
at CNPEM.

The CNPEM is located in Campinas, S=C3=A3o Paulo state, Brazil.

See more details through this link:

https://pages.cnpem.br/trabalheconosco/2020/03/23/researcher-with-a-focus-o=
n-electron-transmission-microscopy-or-atomic-force-191486/
{https://pages.cnpem.br/trabalheconosco/2020/03/23/researcher-with-a-focus-o=n-electron-transmission-microscopy-or-atomic-force-191486/}


This position is likely for the LNNano (Brazilian National Nanotechnology
Laboratory) at CNPEM (https://lnnano.cnpem.br/)


Cheers,

--
Erico Freitas

Physicist/Microscopist at Center of Microscopy
Universidade Federal de Minas Gerais (UFMG)
Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901.
+55-31-3409-7573
+55-31-3409-7575

Coordinator:Transmission Electron Microscopy laboratory

CV Lattes: *http://lattes.cnpq.br/8786127123101199*
{https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#}

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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Apr 2020 08:28:30 -0500
Subject: [Microscopy] viaWWW:Aurox On-line Conference on Microscopy 7th and 8th April 2020

Contents Retrieved from Microscopy Listserver Archives
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Email: phillipa.timmins-at-aurox.co.uk

Name: Phillipa Timmins

Organization: Aurox Ltd

Title-Subject: [Filtered] Aurox On-line Conference on Microscopy 7th and 8th April 2020

Message: Dear All,

I hope you are all staying safe in these troubling and turbulent times. We send out our best wishes
from Aurox.

We have had a hugely positive response to our upcoming On-line Conference on Microscopy on 7th and
8th April.
We have a fantastic line up of speakers from academia and industry covering a range of topics
including: Raman Microscopy, Optical Diffraction Tomography, Cryo-microscopy, Super resolution, Live
cell imaging, AI, Image processing, Adaptive Optics, Mesolens, Expansion Microscopy as well as
keynote by Tony Wilson and updates from various companies.

I just wanted to let you know that the deadline to register your interest in attending is this
Friday at 2PM UK time.

It is free to attend and you can find the program and sign up page here:

http://www.aurox.co.uk/aurox-confocal-microscope-conference.php

I look forward to seeing you all there

Best wishes

Phillipa


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From: microscopy.listserver-at-gmail.com
Date: Wed, 8 Apr 2020 09:08:35 -0500
Subject: Re: [Microscopy] viaWWW:Clinical EM lab

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Email: xbren-at-uw.edu Name: Shirley

Organization: UWMC

Title-Subject: [Filtered] Clinical EM lab

Message: Hello colleagues,
Does anyone know how many clinical EM labs are in America? our department manager would like to
know, and may need help in the future.

Thank you,

Shirley

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From andralbi54nuxdo-at-gmail.com Sat Apr 4 22:18:40 2020
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Email: xbren-at-uw.edu Name: Shirley

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Title-Subject: [Filtered] Clinical EM lab

Message: Hello colleagues,
Does anyone know how many clinical EM labs are in America? our department manager would like to
know, and may need help in the future.

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From: diller-at-stefan-diller.com
Date: Thu, 9 Apr 2020 02:18:31 -0500
Subject: [Microscopy] Re: viaWWW: Vintage DTSA

Contents Retrieved from Microscopy Listserver Archives
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Email: leroy-at-icmpe.cnrs.fr

Name: Eric Leroy

Organization: CNRS

Title-Subject: [Filtered] Vintage DTSA

Message: Hi,
I am searching for the old DTSA software. The web page of the NIST still exists but all the download
links are dead. Does anybody have a backup of DTSA 2.5 or 3.1 ?
Thank you by advance,
Best regards,
Eric

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From solizbula031axig-at-gmail.com Wed Apr 8 17:16:46 2020
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Hello Eric,

if you still need it: I found sea.hqx archive files of PowerDTSAv2.5.1 and PPC_DTSA301 on my computer.

Also the manual.


Best wishes,

Stefan

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Am 08.04.20 um 17:51 schrieb zaluzec-at-microscopy.com:
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} Title-Subject: [Filtered] Vintage DTSA
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From: microscopy.listserver-at-gmail.com
Date: Thu, 9 Apr 2020 06:34:02 -0500
Subject: [Microscopy] viaWWW:SEM sample prep: Critical Point Drying and Sputter Coating

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Title-Subject: [Filtered] SEM sample prep: Critical Point Drying and Sputter Coating

Message: 1) Looking for drying chamber and sputter coating equipment for SEM sample preparation that
can accommodate large specimens (up to 5cm in diameter or up to 8-10cm long with a narrower diameter
such as a large vessel). What equipment would you recommen?

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From: seato008-at-umn.edu
Date: Sat, 11 Apr 2020 11:54:59 -0500
Subject: [Microscopy] SEM images of microbes

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I am a materials scientist and not a biologist, but I will offer some ideas.

I have only seen a couple CPD systems. I don't think they would have been able to accommodate such a large sample. There may be some that could, but I think they would be rare. I recall you will also have issues with thickness. The processing time will depend on the thickness and if all the alcohol is not removed, you will disrupt your structure.

We purchased a Denton sputter coater with a turntable designed to evenly coat samples up to 6 inches in diameter. It worked, but was slower than the standard coater. There was a sectored aperture in front of the target to ensure even coating and that slowed the rate per unit area, and then there was much more area to cover so more time was required.

There may also be issues with the SEM. Our FEI Quanta is the 250 model with a 2-inch stage. Our low-mag limit is 50x at 10 mm working distance, so we can only image 2mm x 1.5mm at a time. We can only survey a 2-inch x 2-inch area at a time. We can load larger samples, but we would have to remove and reset them in order to cover the entire area.

I think you will also find that you may not have good coating over the entire surface. It depends on how convoluted your sample is. You may need to ground the surface in many places to make sure the charge can bleed away to the stage.

With all those issues, I wonder if you might be better off to prepare multiple, smaller samples that can be dehydrated, coated, and imaged using readily available equipment. Hopefully the features of interest would not fall only on the cut lines between the sub-samples.

Also, do you need high magnification to look at small surface structures? If not, the variable pressure or environmental modes of an SEM like the Quanta might not require any sample preparation. It depends on what you want to see.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

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Email: srousselle-at-stagebio.com

Name: Serge Rousselle

Organization: StageBio

Title-Subject: [Filtered] SEM sample prep: Critical Point Drying and Sputter Coating

Message: 1) Looking for drying chamber and sputter coating equipment for SEM sample preparation that can accommodate large specimens (up to 5cm in diameter or up to 8-10cm long with a narrower diameter such as a large vessel). What equipment would you recommen?

2) Anyone able to provide remote/web-based training for SEM sample preparation (not imaging, just sample prep)?
Thank you
Serge

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Listserver Email Form V - 20120416
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From smithejoseph18faoog-at-gmail.com Thu Apr 9 17:47:00 2020
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X-from: Desert Rat {desertrat99-at-verizon.net}

Hi folks,

I have a friend who is trying to identify the features in some SEM images
but they are bio and I am hard materials. The images are at the following
link
https://drive.google.com/drive/folders/1vzKbmfoDZT6f9fsJduTgxx_hwKj1-sGE?usp=sharing
They were collected from a white microbial mat in a cave at Lava Beds
National Monument with NPS permission. They were mounted to SEM stubs with
carbon tape and dried in a vacuum oven. Then coated in gold/palladium and
put in the SEM.
They think the big, 100 micron, thing is a mite? Any other idea welcome.
What they really need to know is what are the little wormy things that look
like chains of balls |--500nm in diameter? They think they might be bacteria
eating the dead mite?

Nick

--

Dr Nicholas Seaton

SEM, FIB & LM Specialist
Department Safety Officer
Characterization Facility

College of Science and Engineering

University of Minnesota

12 Shepherd Labs

100 Union Street SE

Minneapolis

MN, 55455



email: seato008-at-umn.edu

phone: 612-626-5314

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From: wesaia-at-iastate.edu
Date: Sat, 11 Apr 2020 12:43:10 -0500
Subject: [Microscopy] SEM images of microbes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am also a materials guy rather than a biologist.

I am inclined to agree with your identification of a mite of some kind.

I don't think the semicircular features are bacteria. The size may be about right, but I don't think the distribution is. I wonder if it might be some sort of exude from the drying process.

I think the very fine structures in the 3500x and 12,000x images are coating grains. I have seen similar things before where the coating does not "like" the substrate. It "beads up", if you will and creates those recognizable structures. I wonder what an uncoated version of the sample would look like.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
X-from: seato008-at-umn.edu [mailto:seato008-at-umn.edu]
Sent: Saturday, April 11, 2020 11:56 AM
To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

Hi folks,

I have a friend who is trying to identify the features in some SEM images but they are bio and I am hard materials. The images are at the following link https://drive.google.com/drive/folders/1vzKbmfoDZT6f9fsJduTgxx_hwKj1-sGE?usp=sharing
They were collected from a white microbial mat in a cave at Lava Beds National Monument with NPS permission. They were mounted to SEM stubs with carbon tape and dried in a vacuum oven. Then coated in gold/palladium and put in the SEM.
They think the big, 100 micron, thing is a mite? Any other idea welcome.
What they really need to know is what are the little wormy things that look like chains of balls |--500nm in diameter? They think they might be bacteria eating the dead mite?

Nick

--

Dr Nicholas Seaton

SEM, FIB & LM Specialist
Department Safety Officer
Characterization Facility

College of Science and Engineering

University of Minnesota

12 Shepherd Labs

100 Union Street SE

Minneapolis

MN, 55455



email: seato008-at-umn.edu

phone: 612-626-5314

==============================Original Headers==============================
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From: eikonika-at-otenet.gr
Date: Sat, 11 Apr 2020 15:20:23 -0500
Subject: [Microscopy] SEM images of microbes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi
Looks like a mite "fried" (from the lava or the oven?) and its hair look
like a "king size" microbe bearing nice little balls (burnings?) Enjoy!

yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.netwww.aim.cat
*************************************



-----Original Message-----
X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu]
Sent: Saturday, April 11, 2020 20:43
To: eikonika-at-otenet.gr

I am also a materials guy rather than a biologist.

I am inclined to agree with your identification of a mite of some kind.

I don't think the semicircular features are bacteria. The size may be about
right, but I don't think the distribution is. I wonder if it might be some
sort of exude from the drying process.

I think the very fine structures in the 3500x and 12,000x images are coating
grains. I have seen similar things before where the coating does not "like"
the substrate. It "beads up", if you will and creates those recognizable
structures. I wonder what an uncoated version of the sample would look
like.

Warren Straszheim
Materials Analysis and Research Lab
Iowa State University

-----Original Message-----
X-from: seato008-at-umn.edu [mailto:seato008-at-umn.edu]
Sent: Saturday, April 11, 2020 11:56 AM
To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

Hi folks,

I have a friend who is trying to identify the features in some SEM images
but they are bio and I am hard materials. The images are at the following
link
https://drive.google.com/drive/folders/1vzKbmfoDZT6f9fsJduTgxx_hwKj1-sGE?usp
=sharing
They were collected from a white microbial mat in a cave at Lava Beds
National Monument with NPS permission. They were mounted to SEM stubs with
carbon tape and dried in a vacuum oven. Then coated in gold/palladium and
put in the SEM.
They think the big, 100 micron, thing is a mite? Any other idea welcome.
What they really need to know is what are the little wormy things that look
like chains of balls |--500nm in diameter? They think they might be bacteria
eating the dead mite?

Nick

--

Dr Nicholas Seaton

SEM, FIB & LM Specialist
Department Safety Officer
Characterization Facility

College of Science and Engineering

University of Minnesota

12 Shepherd Labs

100 Union Street SE

Minneapolis

MN, 55455



email: seato008-at-umn.edu

phone: 612-626-5314

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From: oshel1pe-at-cmich.edu
Date: Sat, 11 Apr 2020 15:22:13 -0500
Subject: [Microscopy] SEM images of microbes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The big thing looks like an Oribatid mite. The setation (pattern and morphology of the hairs) would probably ID at least the family, but I don't have the necessary keys.
The other 2 images ... hm. The "wormy things" could be bacterial. They might be part of the mite. Some mites do have some pretty intricate decorations.
I don't think the "worms", are a coating artifact - I've never seen an artifact like this, certainly. The smaller rosettes might be coating artifact, but I don't think they are either, the shapes are too intricate and consistent.

What were the coating parameters? Time, pressure, mA?

Phil
-----------------------------------------
Philip Oshel
Imaging Center Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576 office
(989) 774-7567 lab



________________________________________
X-from: seato008-at-umn.edu {seato008-at-umn.edu}
Sent: Saturday, April 11, 2020 13:01
To: Oshel, Philip Eugene

Hi folks,

I have a friend who is trying to identify the features in some SEM images
but they are bio and I am hard materials. The images are at the following
link
https://drive.google.com/drive/folders/1vzKbmfoDZT6f9fsJduTgxx_hwKj1-sGE?usp=sharing
They were collected from a white microbial mat in a cave at Lava Beds
National Monument with NPS permission. They were mounted to SEM stubs with
carbon tape and dried in a vacuum oven. Then coated in gold/palladium and
put in the SEM.
They think the big, 100 micron, thing is a mite? Any other idea welcome.
What they really need to know is what are the little wormy things that look
like chains of balls |--500nm in diameter? They think they might be bacteria
eating the dead mite?

Nick

--

Dr Nicholas Seaton

SEM, FIB & LM Specialist
Department Safety Officer
Characterization Facility

College of Science and Engineering

University of Minnesota

12 Shepherd Labs

100 Union Street SE

Minneapolis

MN, 55455



email: seato008-at-umn.edu

phone: 612-626-5314

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From: microscopy.listserver-at-gmail.com
Date: Tue, 14 Apr 2020 08:25:03 -0500
Subject: [Microscopy] viaWWW:Leo S430 EO Board

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: joern1911-at-gmail.com

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Email: joern1911-at-gmail.com

Name: Joern

Organization: service Sem

Title-Subject: [Filtered] Leo S430 EO Board

Message: Hello Anybody,

please can you Help Please?

I have a Leo S430 SEM.

On my EO Board is a Error on C1 Coil .
i have 3 ampere on the Output.


On my EO-Board is the Vision with 2 OP .
I have found a Schcematic with 1 Op for the C1 Output. I think , the first Op is for the Setpoint
and the second Op is for actual value from the Output currend from the C1 Coil Lens.

Have a anybody a schcematic from the EO_Board.

Thanks and best regards

Joern

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From: tbargar-at-unmc.edu
Date: Tue, 14 Apr 2020 10:37:54 -0500
Subject: [Microscopy] Electron Microscopy Core Facility at UNMC is open

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

We know some institutions are shutting down completely during this pandemic. Our facility is still open (we have been designated as essential and will be required to stay open if at all possible). If you can't access your usual EM facilities, we would be glad to help. We will provide you with a D.O.T. approved shipping container kit for you to use to send your samples to us. If you need help contact me.

Tom Bargar
Electron Microscopy Specialist
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
tbargar-at-unmc.edu
402-559-7347


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.


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From: microscopy.listserver-at-gmail.com
Date: Tue, 14 Apr 2020 19:46:48 -0500
Subject: [Microscopy] viaWWW: Best carbon coating method

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Email: pmccurdy-at-colostate.edu Name: Pat McCurdy

Organization: Colorado State University

Title-Subject: [Filtered] Best carbon coating method

Message: To Whom It May Concern:

We are seeking to purchase a new carbon coater. Our center does EDS and EBSD. We would like to coat
down to 5 nm of carbon. We are looking at both carbon rod coaters as well as a carbon thread coater.
I would appreciate your input on these two types of coaters or any additional kinds that you may
have any experience with.

Thanks,
Pat McCurdy
Colorado State University

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From: microscopy.listserver-at-gmail.com
Date: Fri, 17 Apr 2020 10:31:48 -0500
Subject: [Microscopy] viaWWW: Correcting CL Astigmatism

Contents Retrieved from Microscopy Listserver Archives
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With due disclaimer that (a) I've been doing C coatings mostly for
charge mitigation on FIB Circuit Edit samples, and (b) below are
personal impressions and not a conclusion from any kind of comparative
study:

The best (perceived as smoothest, cleanest, and most uniform) carbon
coatings I've seen were produced by Gatan's PECS system, using ion
sputtering. I haven't operated PECS myself, but coatings made in it for
me were just perfect.

Overall impression is that good cord and rod evaporation coatings
typically come from turbo-pumped systems run by an operator with enough
patience to wait for a full pump-down. I have been using high-vacuum
version of Safematic, and despite of my initial skepticism am very
pleased with it. Automated exchange of evaporation cord is oh so
convenient..

No vested interest in Gatan/AMETEK or Safematic here....

Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 4/14/2020 8:47 PM, microscopy.listserver-at-gmail.com wrote:
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} Title-Subject: [Filtered] Best carbon coating method
}
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}
} We are seeking to purchase a new carbon coater. Our center does EDS and EBSD. We would like to coat
} down to 5 nm of carbon. We are looking at both carbon rod coaters as well as a carbon thread coater.
} I would appreciate your input on these two types of coaters or any additional kinds that you may
} have any experience with.
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} Pat McCurdy
} Colorado State University
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Email: rvancamp-at-kettering.edu

Name: Rick Van Camp

Organization: Kettering University

Title-Subject: [Filtered] Correcting CL Astigmatism

Message: Please understand I am as surprised to post this issue as you are at reading it. This
adjustment is usually performed without incident. However, in reading notes from previous alignment
efforts with our JEOL 2100+ I find/recall that there was a period of time when I found removing the
oval shape of the beam at 40k MAG virtually impossible. This occurred during the latter stages of
our W-filament life. This may be a strong contributing factor. I am hoping some of you can improve
my understanding and problem solving skills associated with this phenomena.

Use of the 2d function pendant controls did not do the trick as they typically do. As I continued
to think about how to remove the CL astigmatism I first reduced the TEM to 20k MAG and experienced
excellent results on several occasions. However, as I continued to think about this issue I
suspected I needed to migrate further up the column. This implied I needed to examine the Gun
Alignment. I find some additional improvement here with adjustments to gun tilt. Despite this, I
would openly state the effort at 20k MAG was a greater gain than the work gun tilt.

Please try to help me understand this concern better and shoot me down as is necessary....I've been
in physical science a long time...I am used to this arcane treatment! If I did something wrong I
need to know. It is that simple.

Also note I am not well skilled with the "Filament Image" function on our microscope but I currently
suspect I have the right understanding regarding the use of this control. Use it at cross over. I
have observed the filament image function used on an SEM before but this was a completely different
image/result so I see no meaningful comparison at this time. I have frequently used the symmetric
structure of Cat's Eye when aligning a W-filament but this has been during the transient conditions
during Beam On/Off. I recorded these observations using my smartphone camera to refer to them as a
learning aide. I obtained 272 h from my initial attempt aligning a K-type filament so I am pleased
with this result but I have a lot to learn.

Thank you for your time.

Rick

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From: microscopy.listserver-at-gmail.com
Date: Fri, 17 Apr 2020 16:03:45 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Correcting CL Astigmatism

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Integrity Scientific Ltd {contact-at-integrityscientific.com}


Hi Rick,

You were probably seeing coma, rather than astigmatism, caused by a badly aligned beam tilt,
mistakenly corrected with beam shift. They can look very similar, but if it is tilt when you hit
the HT wobbler button you will see the beam move across the screen and change shape. Adjust BF tilt
until the wobbling is concentric (you will probably need to adjust beam shift too to keep the beam
on the screen).

Having said this, if you are trying to align alpha 1 rather than alpha 3 the rules are different.
If this is the case let me know and I can give you the more complicated version!
[In the U.K. we are locked down and this is the closest I am going to get to touching a TEM for the
next month I think. Stay safe.]

Best wishes
Richard

} On 17 Apr 2020, at 16:32, microscopy.listserver-at-gmail.com wrote:
}
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} can benefit from our collective wisdom
} ---------------------------------------------------------------------------
}
} Email: rvancamp-at-kettering.edu
}
} Name: Rick Van Camp
}
} Organization: Kettering University
}
} Title-Subject: [Filtered] Correcting CL Astigmatism
}
} Message: Please understand I am as surprised to post this issue as you are at reading it. This
} adjustment is usually performed without incident. However, in reading notes from previous alignment
} efforts with our JEOL 2100+ I find/recall that there was a period of time when I found removing the
} oval shape of the beam at 40k MAG virtually impossible. This occurred during the latter stages of
} our W-filament life. This may be a strong contributing factor. I am hoping some of you can improve
} my understanding and problem solving skills associated with this phenomena.
}
} Use of the 2d function pendant controls did not do the trick as they typically do. As I continued
} to think about how to remove the CL astigmatism I first reduced the TEM to 20k MAG and experienced
} excellent results on several occasions. However, as I continued to think about this issue I
} suspected I needed to migrate further up the column. This implied I needed to examine the Gun
} Alignment. I find some additional improvement here with adjustments to gun tilt. Despite this, I
} would openly state the effort at 20k MAG was a greater gain than the work gun tilt.
}
} Please try to help me understand this concern better and shoot me down as is necessary....I've been
} in physical science a long time...I am used to this arcane treatment! If I did something wrong I
} need to know. It is that simple.
}
} Also note I am not well skilled with the "Filament Image" function on our microscope but I currently
} suspect I have the right understanding regarding the use of this control. Use it at cross over. I
} have observed the filament image function used on an SEM before but this was a completely different
} image/result so I see no meaningful comparison at this time. I have frequently used the symmetric
} structure of Cat's Eye when aligning a W-filament but this has been during the transient conditions
} during Beam On/Off. I recorded these observations using my smartphone camera to refer to them as a
} learning aide. I obtained 272 h from my initial attempt aligning a K-type filament so I am pleased
} with this result but I have a lot to learn.
}
} Thank you for your time.
}
} Rick
}
} Login Host: 68.40.45.131
} Listserver Email Form V - 20120416
} ---------------------------------------------------------------------------
}
}
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From: microscopy.listserver-at-gmail.com
Date: Fri, 17 Apr 2020 16:04:35 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Best carbon coating method

Contents Retrieved from Microscopy Listserver Archives
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X-from: Greg Baty {gbaty-at-pdx.edu}


I have only done carbon rod and an old Gatan PECS ion deposition system.  My
qualitative observations are below and I have no financial interest in coating materials companies.

The PECS is slow, expensive to operate, and for our samples was limited to one at a time, but it
worked well until we stopped maintaining it due to lack of use.

We have a 10 year old EMS rod type turbo pumped carbon coater (not the one they currently sell).  It
works fine down to 3nm on EBSD, but is a bit trickier to get the desired thickness. 
Consistently sharpening the rod is key to getting a consistent thickness. Some of my geology people
complain that they have to run two coating cycles since the sharpened rod will not get them to 25nm
if they are going to microprobe.

If you want a high quality C coat high vacuum is a must.

Greg

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Email: pmccurdy-at-colostate.edu {mailto:pmccurdy-at-colostate.edu} Name: Pat McCurdy

Organization: Colorado State University

Title-Subject: [Filtered] Best carbon coating method

Message: To Whom It May Concern:

We are seeking to purchase a new carbon coater. Our center does EDS and EBSD. We would like to coat
down to 5 nm of carbon. We are looking at both carbon rod coaters as well as a carbon thread
coater.
I would appreciate your input on these two types of coaters or any additional kinds that you may
have any experience with.

Thanks,
Pat McCurdy
Colorado State University

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--
Greg Baty
Center for Electron Microscopy and Nanofabrication
Portland State University
503-725-2867
www.pdx.edu/cemn {http://www.pdx.edu/cemn}

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From: microscopy.listserver-at-gmail.com
Date: Fri, 17 Apr 2020 16:05:05 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Best carbon coating method

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With due disclaimer that (a) I've been doing C coatings mostly for charge mitigation on FIB Circuit
Edit samples, and (b) below are personal impressions and not a conclusion from any kind of
comparative study:

The best (perceived as smoothest, cleanest, and most uniform) carbon coatings I've seen were
produced by Gatan's PECS system, using ion sputtering. I haven't operated PECS myself, but coatings
made in it for me were just perfect.

Overall impression is that good cord and rod evaporation coatings typically come from turbo-pumped
systems run by an operator with enough patience to wait for a full pump-down. I have been using
high-vacuum version of Safematic, and despite of my initial skepticism am very pleased with it.
Automated exchange of evaporation cord is oh so convenient..

No vested interest in Gatan/AMETEK or Safematic here....

Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 4/14/2020 8:47 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: pmccurdy-at-colostate.edu Name: Pat McCurdy
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} Organization: Colorado State University
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} Title-Subject: [Filtered] Best carbon coating method
}
} Message: To Whom It May Concern:
}
} We are seeking to purchase a new carbon coater. Our center does EDS and EBSD. We would like to coat
} down to 5 nm of carbon. We are looking at both carbon rod coaters as well as a carbon thread coater.
} I would appreciate your input on these two types of coaters or any additional kinds that you may
} have any experience with.
}
} Thanks,
} Pat McCurdy
} Colorado State University
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From: microscopy.listserver-at-gmail.com
Date: Fri, 17 Apr 2020 16:05:28 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Best carbon coating method

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Sonic {btracy-at-sonic.net}


Hi!

i vote for sputtered carbon

it overcomes all the other techniques disadvantages its own being cost

Once you try it will never go back

bryan

SIP in Oakland




} On Apr 14, 2020, at 5:55 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: pmccurdy-at-colostate.edu Name: Pat McCurdy
}
} Organization: Colorado State University
}
} Title-Subject: [Filtered] Best carbon coating method
}
} Message: To Whom It May Concern:
}
} We are seeking to purchase a new carbon coater. Our center does EDS and EBSD. We would like to coat
} down to 5 nm of carbon. We are looking at both carbon rod coaters as well as a carbon thread coater.
} I would appreciate your input on these two types of coaters or any additional kinds that you may
} have any experience with.
}
} Thanks,
} Pat McCurdy
} Colorado State University
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From: microscopy.listserver-at-gmail.com
Date: Fri, 17 Apr 2020 17:06:00 -0500
Subject: [Microscopy] Fwd: [Filtered] Re: viaWWW: Best carbon coating method

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Mike Toalson {miketoalson-at-gmail.com}


Patrick - great question for the ListServ.  There are advantages for both
Rod and Thread for Carbon Coating.  There is also e-Beam Carbon and Thermal
Evaporation which are more spendy but can control a more precise and
thinner layer.

If you go with traditional rod or thread coating, then you should get a
system with high vacuum (TMP) to have a finer grain size since you want a
layer down to 5nm.  While a carbon rod can be more precise for very thin
layers and finer grain size, some thread systems can be pulsed so the
coating occurs slower to produce good control of thin layers also.  Thread
systems are also a little easier to use since you don not have to sharpen and
handle delicate carbon rods.  A thread system would be good for new users
that just want a quick conductive coating.

Why compromise though?  There are a couple systems that can do both Rod or
Thread or can add Rod capability at a later date as an upgrade.  We offer
such a coater, see this link:
https://elementpi.com/sputter-coaters-carbon-evaporators/

Good luck with your research!

Mike Toalson

element Pi
833.314.1593
mike.toalson-at-elementpi.com {mailto:mike.toalson-at-elementpi.com}
web www.elementpi.com {http://www.elementpi.com}




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From: david.knecht-at-uconn.edu
Date: Wed, 22 Apr 2020 08:39:54 -0500
Subject: [Microscopy] Phase contrast microscopy

Contents Retrieved from Microscopy Listserver Archives
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X-from: Bil Schneider {wfschneider-at-wisc.edu}

We have a Denton 502A Carbon Coater with a turbo molecular pump and Cressington thickness monitor.
Using carbon rods we deposit between 7-10 nm for CL and 20 nm for imaging, EDS, and EPMA. It has a
cold finger so liquid nitrogen can be added to hasten a quick coat, otherwise an hour pump down gets
us to 10 to the minus 6 torr. The stage rotates as its a line of site coater, 10 one inch rounds or
maybe 5 thin sections can go in at one pump down.

We do a ton of EBSD, but rely on a Leica Ace600 coater to apply a 1nm coat of Iridium to any EBSD
sample. It is flawless on geological thin sections and never charges.

Bil Schneider UW Madison Geosciences SEM Lab Manager
wfschneider-at-wisc.edu


} On Apr 14, 2020, at 8:26 PM, microscopy.listserver-at-gmail.com wrote:
}




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Email: pmccurdy-at-colostate.edu Name: Pat McCurdy

Organization: Colorado State University

Title-Subject: [Filtered] Best carbon coating method

Message: To Whom It May Concern:

We are seeking to purchase a new carbon coater. Our center does EDS and EBSD. We would like to coat
down to 5 nm of carbon. We are looking at both carbon rod coaters as well as a carbon thread coater.
I would appreciate your input on these two types of coaters or any additional kinds that you may
have any experience with.

Thanks,
Pat McCurdy
Colorado State University

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From anittiff04vihu-at-gmail.com Sun Apr 19 18:31:03 2020
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Message-ID: {1FC10D74.CB56BAC3-at-gmail.com}

Hello dear colleagues!


Specially to my american fellows, I want to offer my compassion and sympathy in the hard times you (we) are currently experiencing.
I have a specific question regarding the preparation of biological material containing minerals for FIB-SEM (look at it  like for example the examination of wound skin biopsies upon application of submicroparticles).
The purpose is to etch a block parallel to the surface and sequentially using a FIB-SEM (like horizonzal sectionning but with a FIB) and looking for mineral particles inside the block, building a 3D image of the block.
We performed a preliminary experiment showing that the particles can be easily detected (particle contrast and resolution are good enough). 
However, we faced 2 problems:
(1) paraffin is etched much too fast compared to the particles inside the sample
(2) we got a strong charging effect


So now I am looking for alternative embedding material which could replace paraffin.
Does anyone have any experiences with epoxy blocks in FIB-SEM? 
Can I expect epoxy resins to be harder to etch than paraffin? 
Is there a rule to a priori calculate the "hardness" (FIB-etchiness) of a material to be etched to match the embedding material to the particles embedded inside?
How can I make an epoxy resin conductive? I thought above mixing silver nanoparticles in the embedding material (they wouldn't disturb the analysis because they are very different from the particles we are looking for).
Does anyone have an experience to share?
Thank you in advance and stay safe!


Best regards,
Stephane



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I thought I understood how phase contrast microscopy works, but then I was reading MicroscopyU (https://www.microscopyu.com/techniques/phase-contrast/introduction-to-phase-contrast-microscopy) and other sites and now I am confused. My understanding is that it works because of refractive index differences in different parts of the light path (cells) leading to retardation of refracted light and eventual phase differences relative to un-refracted (surround) light that passes through the thinned area of the phase plate in the objective. However, in the web site indicated and others, they use refracted light and diffracted light almost interchangeably in explaining phase contrast. To my “biologist” level understanding, diffraction and refraction are very different phenomenon and I did not think that diffraction changed the optical path length like refractive index differences. Refraction makes total sense to me in the context of phase contrast, but I don’t see how diffraction is relevant. Can someone explain what I am missing? Thanks, Dave

Dr. David Knecht
Professor, Department of Molecular and Cell Biology
University of Connecticut
91 N. Eagleville Rd.
U-3125
Storrs, CT 06269-3125





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From: nizets2-at-yahoo.com
Date: Wed, 22 Apr 2020 09:20:14 -0500
Subject: [Microscopy] Re: Phase contrast microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi David!

I am a biologist too and sometimes have a hard time to understand such complex physical phenomena, so perhaps I can use the right words for you to understand.

My understanding is:
- Diffraction is due to the interaction of the focused incoming light on the sample. Diffraction is the reason why an object is visible in light and electron microscopy.
- Refraction is related to the change in light speed through a change in the medium (lense) thickness, here the phase ring (which you must choose according to the objective used).

The principle here is to separate in physical space the normal (not diffracted) light from the light diffracted by the sample.
If you look at the picture shown in the link you gave, you see only a yellow beam before the sample but after the sample you see a light pink color between (and also outside) the yellow beams (called direct surround light). This is the diffracted light (the lines point to the rosy color between the yellow light beams).

I think I identified your problem : the phase difference doesn't occur by passing through the sample, it occurs due to the phase ring placed after (or within) the objective lense. The interaction of light with the sample give you diffracted, not refracted light (or perhaps some negligible refraction).
 
The diffracted light (by the specimen) will be refracted by the objective lense just as the non-diffracted light is BUT it will not strike the lense at the same place. Only the non-diffracted light with pass through the phase ring (because the ring is calculated to be in the path of non-diffracted light only) and this will result in a phase difference between the light diffracted by the specimen and the light that passes through it unchanged. 
This difference is then reconstructed on the image plane to give a contrast.

I hope I could help, it is hard to explain a 3D view only with words.

Stay safe and don't hesitate to ask for more explanations

Stephane





On Wednesday, April 22, 2020, 03:48:03 PM GMT+2, david.knecht-at-uconn.edu {david.knecht-at-uconn.edu} wrote:








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I thought I understood how phase contrast microscopy works, but then I was reading MicroscopyU (https://www.microscopyu.com/techniques/phase-contrast/introduction-to-phase-contrast-microscopy) and other sites and now I am confused.  My understanding is that it works because of refractive index differences in different parts of the light path (cells) leading to retardation of refracted light and eventual phase differences relative to un-refracted (surround) light that passes through the thinned area of the phase plate in the objective.  However, in the web site indicated and others, they use refracted light and diffracted light almost interchangeably in explaining phase contrast.  To my “biologist” level understanding, diffraction and refraction are very different phenomenon and I did not think that diffraction changed the optical path length like refractive index differences.  Refraction makes total sense to me in the context of phase contrast, but I don’t see how diff!
raction is relevant. Can someone explain what I am missing?  Thanks, Dave

Dr. David Knecht
Professor, Department of Molecular and Cell Biology
University of Connecticut   
91 N. Eagleville Rd.
U-3125
Storrs, CT 06269-3125





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From: vray-at-partbeamsystech.com
Date: Wed, 22 Apr 2020 09:20:23 -0500
Subject: [Microscopy] Re: Phase contrast microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

With due disclaimer that I am doing ion and electron beam processing and
my recollection of light optics is from loooong time ago - diffraction
encompasses multitude of phenomena, including separating spectral
components of white light and possible interference between waves
diffracted at different locations of the object. Separation of the
spectrum with white light illumination and interference could both
produce delayed wavefront resulting in phase contrast.

My feeling however is that most of phase contrast from thin, uniform,
transparent biological specimens would be produced by dirrerences in the
index of refraction...

"Diffracted light" notation on microscopyu page refers to separation of
diffraction orders within the light path of the microscope. During
diffraction contrast imaging direct light (DC background) is blocked by
illumination aperture (condenser annulus) and the diffraction plate,
while first-order diffracted light is passed to image plane to form
phase-contrast images.

I'm sure that many people with more current and fundamental knowledge of
optical microscopy will correct and expand my qualitative and
exceedingly hand-waving explanation...

Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
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www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 4/22/2020 9:40 AM, david.knecht-at-uconn.edu wrote:
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} I thought I understood how phase contrast microscopy works, but then I was reading MicroscopyU (https://www.microscopyu.com/techniques/phase-contrast/introduction-to-phase-contrast-microscopy) and other sites and now I am confused. My understanding is that it works because of refractive index differences in different parts of the light path (cells) leading to retardation of refracted light and eventual phase differences relative to un-refracted (surround) light that passes through the thinned area of the phase plate in the objective. However, in the web site indicated and others, they use refracted light and diffracted light almost interchangeably in explaining phase contrast. To my “biologist” level understanding, diffraction and refraction are very different phenomenon and I did not think that diffraction changed the optical path length like refractive index differences. Refraction makes total sense to me in the context of phase contrast, but I don’t see how diff!
} raction is relevant. Can someone explain what I am missing? Thanks, Dave
}
} Dr. David Knecht
} Professor, Department of Molecular and Cell Biology
} University of Connecticut
} 91 N. Eagleville Rd.
} U-3125
} Storrs, CT 06269-3125
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} 6, 74 -- From: "Knecht, David" {david.knecht-at-uconn.edu}
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From: vray-at-partbeamsystech.com
Date: Wed, 22 Apr 2020 09:20:23 -0500
Subject: [Microscopy] Re: Phase contrast microscopy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

With due disclaimer that I am doing ion and electron beam processing and
my recollection of light optics is from loooong time ago - diffraction
encompasses multitude of phenomena, including separating spectral
components of white light and possible interference between waves
diffracted at different locations of the object. Separation of the
spectrum with white light illumination and interference could both
produce delayed wavefront resulting in phase contrast.

My feeling however is that most of phase contrast from thin, uniform,
transparent biological specimens would be produced by dirrerences in the
index of refraction...

"Diffracted light" notation on microscopyu page refers to separation of
diffraction orders within the light path of the microscope. During
diffraction contrast imaging direct light (DC background) is blocked by
illumination aperture (condenser annulus) and the diffraction plate,
while first-order diffracted light is passed to image plane to form
phase-contrast images.

I'm sure that many people with more current and fundamental knowledge of
optical microscopy will correct and expand my qualitative and
exceedingly hand-waving explanation...

Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
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www.partbeamsystech.com www.freudlabs.com
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479

On 4/22/2020 9:40 AM, david.knecht-at-uconn.edu wrote:
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} I thought I understood how phase contrast microscopy works, but then I was reading MicroscopyU (https://www.microscopyu.com/techniques/phase-contrast/introduction-to-phase-contrast-microscopy) and other sites and now I am confused. My understanding is that it works because of refractive index differences in different parts of the light path (cells) leading to retardation of refracted light and eventual phase differences relative to un-refracted (surround) light that passes through the thinned area of the phase plate in the objective. However, in the web site indicated and others, they use refracted light and diffracted light almost interchangeably in explaining phase contrast. To my “biologist” level understanding, diffraction and refraction are very different phenomenon and I did not think that diffraction changed the optical path length like refractive index differences. Refraction makes total sense to me in the context of phase contrast, but I don’t see how diff!
} raction is relevant. Can someone explain what I am missing? Thanks, Dave
}
} Dr. David Knecht
} Professor, Department of Molecular and Cell Biology
} University of Connecticut
} 91 N. Eagleville Rd.
} U-3125
} Storrs, CT 06269-3125
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From: microscopy.listserver-at-gmail.com
Date: Wed, 29 Apr 2020 10:33:24 -0500
Subject: [Microscopy] viaWWW:Third Party Service for TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Colleagues

As one of the symposium organizers for EMC 2020 23-28 August in Copenhagen I have
just received the announcement that the meeting has been canceled as per the
announcement from the Danish Government. The link to the official announcement
prohibiting meetings of more than 500 people until after Sept 1 is below.

https://sum.dk/Aktuelt/Nyheder/Coronavirus/2020/April/Regerinen-fastsaetter-loft-paa-store-forsamlnger-paa-500-deltagere.aspx

I also have receive confirming Email from the Royal Microscopy Society of the cancellation.

The "rough translation" of the Danish announcement on the above WWW site is:

---------------------------------------------------------------------------------------------------------
PRESS RELEASE - The government has decided to set an upper limit for assemblies of 500 people. It
will be valid until 1 September 2020.

The government has decided that a ban on major events will be prohibited over the summer and until 1
September 2020. The limit for large events has now been set so that events with more than 500 people
may not be held. The decision is made as a result of the health authorities' assessment that the
corona epidemic will continue to develop in Denmark over the coming months. However, setting the
ceiling of 500 people for large events will not have any practical significance in the first place.

The ban on gathering more than 10 people was extended on Friday up to and including 10 May 2020.

The government closely monitors the development of the epidemic and therefore also adjusts the limit
on how many people are allowed to congregate on an ongoing basis.

Contact Info:
Ministry of Health and Senior Services, Press Telephone: 21 32 47 27
---------------------------------------------------------------------------------------------------------


Nestor
Your Friendly Neighborhood SysOp


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From koepketemi17uno-at-gmail.com Thu Apr 23 04:31:23 2020
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Email: smodla-at-udel.edu Name: Shannon Modla

Organization: University of Delaware

Title-Subject: [Filtered] Third Party Service for TEM

Message: The manufacturer of our TEM will be ending service within two years. It's a 120kV LaB6 TEM
running on Windows XP. Does anyone have advice or can recommend another company that would provide
service to the instrument until we are able to acquire funds for a replacement?

Thanks,

Shannon
Research Associate III
University of Delaware

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From: microscopy.listserver-at-gmail.com
Date: Wed, 29 Apr 2020 17:24:01 -0500
Subject: [Microscopy] viaWWW:Virtual M&M 2020 or emc 2020?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

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Email: john.hyun-at-ametek.com

Name: John Hyun

Organization: Gatan

Title-Subject: [Filtered] Virtual M&M 2020 or emc 2020?

Message: We hope you are safe and well. In lieu of a live conference, would you be interested in a
virtual 2020 M&M Meeting or virtual emc 2020? M&M is still scheduled as a live event. These are our
industry's premiere events, and it would be a shame to cancel one or both without a real option to
"attend." Fantastic virtual platforms and management companies are available. This will be the
future of events.

If you would like to see M&M and emc go virtual, we encourage you to email the meeting managers (see
below) with your support. Lets meet in a safe and dynamic way!

M&M 2020: Nicole Guy; nguy-at-conferencemanagers.com
emc 2020: Allison Winton; awinton-at-rms.org.uk

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From: microscopy.listserver-at-gmail.com
Date: Thu, 21 May 2020 18:45:59 -0500
Subject: [Microscopy] viaWWW: Eyepiece cameras for TEM as a focusing aid/demo tool?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Mike Marko {mike.marko.em-at-gmail.com}

If it is a JEOL, try Joe Bricker. I'm sure some ex-engineers from other companies can handle those
EMs. Start by asking your regular service engineer whom he might know.

On 4/29/2020 11:37 AM, microscopy.listserver-at-gmail.com wrote:
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} Email: smodla-at-udel.edu Name: Shannon Modla
}
} Organization: University of Delaware
}
} Title-Subject: [Filtered] Third Party Service for TEM
}
} Message: The manufacturer of our TEM will be ending service within two years. It's a 120kV LaB6 TEM
} running on Windows XP. Does anyone have advice or can recommend another company that would provide
} service to the instrument until we are able to acquire funds for a replacement?
}
} Thanks,
}
} Shannon
} Research Associate III
} University of Delaware
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From gunnclar77z-at-gmail.com Fri May 1 16:30:21 2020
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X-from: Raffaella.carzaniga-at-crick.ac.uk

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Email: Raffaella.carzaniga-at-crick.ac.uk

Name: Raffa Carzaniga

Organization: The Francis Crick Institute

Title-Subject: [Filtered] Webinar: Imaging SARS-CoV-2 safely: Protecting the microscopy community
(Friday 22nd May 13:00-16:00)

Message: Dear Colleagues,
A webminar about Imaging SARS-CoV-2 safely: Protecting the microscopy community, which will
take place virtually on Friday 22nd May from 13:00 to 16:00 BST.
Please find further details below, and visit the following link to register:

https://www.crick.ac.uk/whats-on/webinarimaging-sars-cov-2-safely-protecting-the-uk-microscopy-community


Meeting description:
As the SARS-CoV-2 pandemic progresses, scientific focus will gradually shift from frontline testing
and tracking capability, to studying the fundamental cell biology of the virus and clinical
progression of virus infection in human tissues. Imaging virus-infected cells and tissues with
light, electron and X-ray microscopes will play a critical role in understanding the pathogen and in
designing future vaccines and therapies. In this context, it is likely that research and diagnostic
imaging facilities will soon face requests from researchers and clinicians to handle virus-infected
samples. It is therefore critical that we understand how to safely handle these samples, and ensure
that we protect the UK microscopy community from lab-acquired infections. This webinar brings
together expert pathologists, microscopists and virologists who are studying inactivation of virus
in cells and tissues for safe handling, imaging the cell biology of viruses, and handling
virus-infected human tissues. Invited speakers will give short talks sharing their expertise,
followed by a Q&A session to answer questions from the microscopy community.
Programme
13:00 - 13:10 Introduction and webinar etiquette
Lucy Collinson (Francis Crick Institute, London, UK)
13:10 - 13:30 Looking CoViD-19 in the eye: a perspective from an NHS histopathologist
Dr Emyr Wyn Benbow (University of Manchester/Manchester Royal Infirmary)
13:30 - 13:50 Virus Inactivation for Imaging how to get images of SARS-CoV-2 without
endangering yourself or the community
Dr Matthew Hannah (Public Health England)
13:50 - 14:10 Imaging viruses in high containment research labs
Professor Pippa Hawes (The Pirbright Institute)
14:10 - 14:30 Understanding coronavirus replication organelles
Dr Helena Maier (The Pirbright Institute)
14:30 - 14:50 The virologists toolbox. An overview of methods to manipulate RNA viruses and
cells for imaging
Dr Rachel Ulferts (Francis Crick Institute)
14:50 - 16:00 Panel Discussion/ Q&A
All

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Email: vitha-at-tamu.edu

Name: Stanislav Vitha

Organization: Texas A&M University

Title-Subject: [Filtered] Eyepiece cameras for TEM as a focusing aid/demo tool?

Message: I was wondering if anybody has an experience or opinion on using an eyepiece camera (it
would replace one of the eyepieces in the focusing binoculars on the TEM) as an aid or a
demonstration/training tool on TEMs that do not have the live view option for their imaging cameras.

I could see it being used when demonstrating astigmatism (and stigmation), and focusing. There are
some monochrome eyepiece cameras that are used for hobby astrophotography.
Is it a bad idea or could it actually be useful?

Thank you!

Stan Vitha
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From: surya.rout-at-tuhh.de
Date: Fri, 22 May 2020 06:19:47 -0500
Subject: [Microscopy] Looking For Used FEI (CM-series) TEM (TU Hamburg)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stanislav,

Do you have a digital camera on your TEM? Even the lower end ones should be able to generate a frame rate adequate for illustrative movies. You can work at frame rates as low as 4 frames/sec and still be able to see the process. You can also reduce your pixel resolution (512x512te is adequate) on the camera (binning) to increase the frame rate if necessary.

I've done this with a camera that didn't have movie recording capability and I couldn't install a recording program on the instrument PC. I had, however, a program that allowed me to record the screen on my laptop. I used VNC to remote into my TEM PC and then recorded my laptop screen.

God luck,
Henk

----------------


Hendrik O. Colijn
Center forElectronMicroscopy andAnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Sent: Thursday, May 21, 2020 7:51 PM
To: Colijn, Hendrik {colijn.1-at-osu.edu}

Dear All,

We are looking for a used CM-series or Tecnai-series FEI microscope
that someone wants to decommission.

The TEM will be used in our laboratory for beginner training purpose
and to use as a backup machine for basic imaging purpose. The costs of
transport will be covered by an intermediate company that we have
contacted and the microscope can be in Europe, United States of
America or any other part of the world.

Please message me personally if you are planning to offer such a microscope.

Thank you

Best regards,
Surya

----------------------------------------------------------------------------------


Dr. Surya Snata Rout
M-26, Betriebseinheit Elektronenmikroskopie
Technische Universität Hamburg
(Hamburg University of Technology)
Eißendorfer Straße 42
21073 Hamburg
Germany

Tel.: +49 (0) 40 - 428 78 4881




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From: crwinkler-at-ncsu.edu
Date: Fri, 22 May 2020 08:40:53 -0500
Subject: [Microscopy] Re: viaWWW: Eyepiece cameras for TEM as a focusing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Stan,

It can be a good idea, yes. FEI sold a green screen cover with integrated
Logitech webcam as part of the TARO (Tecnai advanced remote operation)
package, and it can be used for the purpose you described. We have a bottom
mount digital so we just use that. An eyepiece camera should work, assuming
you can match the focal length to the optics of the stereoscope or get an
adjustable one. Ideally you can get one that easily pops off and on, or
work with your machine shop to make a quick change adapter. You don't
realize how often you use the little screen and binoculars until you can't.

As Henk mentioned, you can simulate a live view by binning your camera
down and snapping pictures at very low exposure times. You can use a
mouse control macro on Windows to automatically click the capture
button, and even automatically generate a FFT from each image.

For aberrations in STEM mode, there are simulation packages that can
also be used to show
aberrations in the Ronchigram like Dr. Probe. I really enjoy these as
training tools as you can adjust them, making them worse then better, so the
users can see how aberrations affect the Ronchigram and play around
offline. You are probably talking about TEM mode, though, so maybe not
so useful.

When I was in charge of an EM420 with no live view camera, I got lazy and
just adjusted objective stig and told users not to change it. It was stable
for many weeks and we did not have the budget for a live camera or even
eyepiece camera. Focusing was pretty easy to demonstrate without the live
view.

Good luck,
Chris


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} Title-Subject: [Filtered] Eyepiece cameras for TEM as a focusing aid/demo tool?
}
} Message: I was wondering if anybody has an experience or opinion on using an eyepiece camera (it
} would replace one of the eyepieces in the focusing binoculars on the TEM) as an aid or a
} demonstration/training tool on TEMs that do not have the live view option for their imaging cameras.
}
} I could see it being used when demonstrating astigmatism (and stigmation), and focusing. There are
} some monochrome eyepiece cameras that are used for hobby astrophotography.
} Is it a bad idea or could it actually be useful?
}
} Thank you!
}
} Stan Vitha
} Login Host: 128.194.151.97
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--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
https://www.aif.ncsu.edu/
Cell: 267-496-0587

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11, 53 -- Subject: Re: [Microscopy] viaWWW: Eyepiece cameras for TEM as a focusing
11, 53 -- aid/demo tool?
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From: microscopy.listserver-at-gmail.com
Date: Tue, 26 May 2020 06:34:29 -0500
Subject: [Microscopy] viaWWW: Job posting- TEM Research Technician position at the shared

Contents Retrieved from Microscopy Listserver Archives
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X-from: Mike Marko {mike.marko.em-at-gmail.com}


It would be ideal if such a camera could provide a real-time power spectrum. That would depend on
the granularity of the viewing-screen phosphor and the magnification used.




On 5/21/2020 7:48 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: vitha-at-tamu.edu
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} Name: Stanislav Vitha
}
} Organization: Texas A&M University
}
} Title-Subject: [Filtered] Eyepiece cameras for TEM as a focusing aid/demo tool?
}
} Message: I was wondering if anybody has an experience or opinion on using an eyepiece camera (it
} would replace one of the eyepieces in the focusing binoculars on the TEM) as an aid or a
} demonstration/training tool on TEMs that do not have the live view option for their imaging cameras.
}
} I could see it being used when demonstrating astigmatism (and stigmation), and focusing. There are
} some monochrome eyepiece cameras that are used for hobby astrophotography.
} Is it a bad idea or could it actually be useful?
}
} Thank you!
}
} Stan Vitha
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Email: bif.manager-at-ubc.ca

Name: Miki Fujita

Organization: Bioimaging facility, University of British Columbia, Vancouver Canada

Title-Subject: [Filtered] Job posting- TEM Research Technician position at the shared facility

Message: The Bioimaging facility at University of British Columbia (Vancouver, Canada) is currently
searching for a full time TEM Research Technician.

We are looking for a staff member with expertise in TEM, cryo-TEM and electron tomography at the
Bioimaging Facility at University of British Columbia.
Link to job advertisement:
https://www.hr.ubc.ca/careers-postings/staff-s.php
Job ID # is 37507
Closing date: June 15th, 2020
Facility website: www.bioimaging.ubc.ca





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From: diller-at-stefan-diller.com
Date: Wed, 27 May 2020 10:56:36 -0500
Subject: [Microscopy] Re: CM10 Pre-Installation Manual - Solved

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

I am looking for a Pre-Installation Manual on a Philips/FEI/ThermoFisher CM10 TEM.

Does anybody have one available in PDF?

I would appreciate it very much.


Thanks,

Stefan

--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


==============================Original Headers==============================
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12, 24 -- From: Stefan Diller {stefan.diller-at-t-online.de}
12, 24 -- Subject: CM10 Pre-Installation Manual
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From buviregi380yjnbn-at-gmail.com Wed May 27 07:02:02 2020
Return-Path: {buviregi380yjnbn-at-gmail.com}
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for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 27 May 2020 07:02:02 -0500
Message-ID: {D3672C55.685A55AE-at-gmail.com}

Thanks, I got a CM100 Pre-Installation manual.


Best wishes,

Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 27.05.20 um 10:39 schrieb stefan.diller-at-t-online.de:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Dear All,
}
} I am looking for a Pre-Installation Manual on a Philips/FEI/ThermoFisher CM10 TEM.
}
} Does anybody have one available in PDF?
}
} I would appreciate it very much.
}
}
} Thanks,
}
} Stefan
}

==============================Original Headers==============================
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From: John.Mardinly-at-asu.edu
Date: Wed, 27 May 2020 12:38:34 -0500
Subject: [Microscopy] Kavli Prize awarded to Harald Rose, Max Haider, Knut Urban and Ondrej

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

http://kavliprize.org/prizes-and-laureates/prizes/2020-kavli-prize-nanoscience

A. John Mardinly, Ph.D., P.E.





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From: microscopy.listserver-at-gmail.com
Date: Wed, 27 May 2020 17:57:26 -0500
Subject: [Microscopy] viaWWW:Ultrastructural differences between carcinoma and sarcoma

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Name: Ravi Thakkar

Organization: Kansas State University

Title-Subject: [Filtered] Ultrastructural differences between carcinoma and sarcoma cells.

Message: Can anyone help me to find the difference between carcinoma and sarcoma cells under TEM and
SEM?

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From: microscopy.listserver-at-gmail.com
Date: Thu, 28 May 2020 07:57:09 -0500
Subject: [Microscopy] viaWWW:Now available on demand:- Imaging SARS-CoV-2 safely:

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Name: Raffa Carzaniga

Organization: The Francis Crick Institute

Title-Subject: [Filtered] Now available on demand:- Imaging SARS-CoV-2 safely: Protecting the
microscopy community (Friday 22nd May 2020)

Message: Dear all,
The webinar: Imaging SARS-CoV-2 safely: Protecting the microscopy community is now available on
demand here the link:

https://www.crick.ac.uk/whats-on/webinarimaging-sars-cov-2-safely-protecting-the-microscopy-community
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From: tbargar-at-unmc.edu
Date: Fri, 29 May 2020 09:03:54 -0500
Subject: [Microscopy] How to measure productivity in an EM core facility

Contents Retrieved from Microscopy Listserver Archives
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Dear Listers,

Our Vice Chancellor of Research asked us to develop metrics to evaluate productivity and growth of our electron microscopy core facility. We would like to hear if you have developed specific metrics to assess operations of your facility and what you have learned about the utility and value of different metrics. We appreciate input from any facility, even if you have not established metrics for your program.

Tom Bargar
Electron Microscopy Specialist
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
tbargar-at-unmc.edu
402-559-7347


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.


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From: microscopy.listserver-at-gmail.com
Date: Tue, 2 Jun 2020 08:33:36 -0500
Subject: [Microscopy] viaWWW: 2 PhD positions in Liquid Phase Electron Microscopy - apply

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers

We have a local physics laboratory that might be interested in acquiring a used TEM or SEM (working condition including computer support) to test novel electron sources and develop new electron imaging modalities. We would like to know if anyone has an instrument available in storage or is preparing to decommission an electron microscope.


Tom Bargar
Electron Microscopy Specialist
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
tbargar-at-unmc.edu
402-559-7347



The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.


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From smithejoseph18a-at-gmail.com Fri May 29 09:50:15 2020
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Email: krmo-at-dtu.dk

Name: Kristian Mlhave

Organization: DTU Nanolab, Technical University of Denmark

Title-Subject: [Filtered] 2 PhD positions in Liquid Phase Electron Microscopy - apply before 21st June

Message: Are you or someone you know intersted in t studying nanoscale processes live in the liquid
phase?

We have two PhD positions with international collaboration open where you can apply before 21st June
2020:

PhD scholarship in Nanochannel Liquid Cells for in situ Biochemical Phase Change Studies, in
collaboration with Nanyang Tech. Uni. Singapore.
https://www.nanolab.dtu.dk/english/aboutus/organisation/vacant-positions/job?id=b856978d-3f3b-4f92-8558-03a36138b9c0

PhD scholarship in Charges and Potentials in Liquid Phase Electron Microscopy, in collaboration with
the Ernst-Ruska Center in Germany and Paul Alivisatos' group at UC Berkeley:

https://www.nanolab.dtu.dk/english/aboutus/organisation/vacant-positions/job?id=c0873787-0a8b-44d2-a265-2038ddc5eece

More information on our group on www.nanolab.dtu.dk/mowin Best regards
Kristian Mlhave

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From: eikonika-at-otenet.gr
Date: Mon, 8 Jun 2020 00:53:40 -0500
Subject: [Microscopy] JSM 5600LV SEM fussy image problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Folks -

Just want to mention a Kickstarter project that some of you
might like:

Kickstarter: LadyBug

"An up-to-5-axis motorized macro camera for less than the
cost of a single conventional lens. "

Please do not consider this a "commercial plug", as it is
a DIY project from a college kit with lots of skills at an
affordable price.

Also, a plug for the Delta College video on YouTube:
"Electron microscopy: Celebrating 50 years at Delta College"

regards,
- Jim

==============================Original Headers==============================
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From doripama101ja-at-gmail.com Fri Jun 5 10:56:01 2020
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Dear All
We have a puzzling image problem with our JSM 5600LV SEM: We achieve a
decent image at say X20K; then we lower the magnification at X50 or beyond
and keep it there for about minute and then return it to X20K; now the
image is very fussy and there is no way to clear it with focus or stigma;
but if we wait for about 5 minutes without doing anything the image clears
up by itself.

We have also noticed that when we are at high mag with clear image and apply
stigma reset the stigma shifts too much and needs many button turns to get
corrected.

Since we have a second working JSM 5600LV with same software version (2.05)
we exchanged all boards between them but there was no effect on either
microscope, so we think the problem is not there. Also we open the column of
the problematic scope but couldnt see anything wrong.

Any idea of what could be the problem will be greatly appreciated
Best regards
yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.netwww.aim.cat
*************************************




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8, 21 -- From eikonika-at-otenet.gr Mon Jun 8 00:53:39 2020
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8, 21 -- To: {microscopy-at-microscopy.com}
8, 21 -- Subject: JSM 5600LV SEM fussy image problem
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From: diller-at-stefan-diller.com
Date: Mon, 8 Jun 2020 01:45:50 -0500
Subject: [Microscopy] Re: JSM 5600LV SEM fussy image problem

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good morning, Yorgos,

first: Had this happened at high-vac condition? Did you check the effect on another sample? There might be outgassing or an effect
of specimen preparation.

Another thing could be the beam situation at low and high mag: different diameter with the chance that the more intense low mag
beam might produce charging in the scope inliner.

And there is the good chance the the photomultiplier tube / scintillator has seen "too much light". Normally this shows up as more
noise, streaky patterns in the image.

I would do a check-up with a non-biological specimen, check at some kx magnification with a larger spotsize setting, and see if
you can reproduce the effect at another set of magnifications.

If yes, you should next clean the inliner and change apertures. And / or change the SE detector head.


Best,

Stefan

-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------

Am 08.06.20 um 08:01 schrieb eikonika-at-otenet.gr:
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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}
} Dear All
} We have a puzzling image problem with our JSM 5600LV SEM: We achieve a
} decent image at say X20K; then we lower the magnification at X50 or beyond
} and keep it there for about minute and then return it to X20K; now the
} image is very fussy and there is no way to clear it with focus or stigma;
} but if we wait for about 5 minutes without doing anything the image clears
} up by itself.
}
} We have also noticed that when we are at high mag with clear image and apply
} stigma reset the stigma shifts too much and needs many button turns to get
} corrected.
}
} Since we have a second working JSM 5600LV with same software version (2.05)
} we exchanged all boards between them but there was no effect on either
} microscope, so we think the problem is not there. Also we open the column of
} the problematic scope but couldnt see anything wrong.
}
} Any idea of what could be the problem will be greatly appreciated
} Best regards
} yorgos
}
} Dr Yorgos Nikas
} Athens Innovative Microscopy
} Skra 36 Voula 16673 GREECE
}
} Tel/fax +30 210 8957677
} mobile +30 6945 107477
} www.eikonika.netwww.aim.cat
} *************************************
}
}
}
}
} ==============================Original Headers==============================
} 8, 21 -- From eikonika-at-otenet.gr Mon Jun 8 00:53:39 2020
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} 8, 21 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr}
} 8, 21 -- To: {microscopy-at-microscopy.com}
} 8, 21 -- Subject: JSM 5600LV SEM fussy image problem
} 8, 21 -- Date: Mon, 8 Jun 2020 08:57:44 +0300
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==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Tue, 9 Jun 2020 06:50:39 -0500
Subject: [Microscopy] viaWWW: Leica EM Trim schematics?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

I have a new position within our company, and would appreciate if you can unsubscribe me from the mailing list.

Met vriendelijke groet | Kind regards,

Jack Houet
Supervisor Manufacturing
TEM Test Krios

Thermo Fisher Scientific - MSD
Achtseweg Noord 5, Eindhoven | The Netherlands
M: +31(0)611538706 | E: jack.houet-at-thermofisher.com

-----Original Message-----
X-from: diller-at-stefan-diller.com {diller-at-stefan-diller.com}
Sent: Monday, June 8, 2020 8:52 AM
To: Houet, Jack {jack.houet-at-thermofisher.com}

Yorgos -

I suspect you have something in the beam path
that receives charge at low magnification because
of the wide sweep at low mag.

However, I do not know enough about the JSM 5600LV
to give suggestions on cleaning the column.

However, I would suggest trying to put in a very large
metal sample, to eliminate the charging on the sample
holder. Also, double check the grounding on the sample
holder.

regards,
- Jim

==============================Original Headers==============================
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Email: alice.dohnalkova-at-pnnl.gov

Name: Alice Dohnalkova

Organization: Pacific Northwest National Laboratory

Title-Subject: [Filtered] Leica EM Trim schematics?

Message: Dear fellow microscopists,

My lab has a Leica EM Trim tool (the 'old' one, not EM Trim2), and the advance wheel on the front
has stopped working.
I will ask our machine shop guys to fix it, but it would be great to have the EM Trim schematics
ahead for them.

I realize this is a 20+ year old instrument, but if anyone still kept the brochure, I'd really
appreciate your help sharing it.

Best regards,
Alice.

Alice Dohnalkova, Ph.D.
ENVIRONMENTAL MOLECULAR SCIENCES LABORATORY Pacific Northwest National Laboratory 902 Battelle
Boulevard P.O. Box 999, MSIN K8-93 Richland, WA 99352 USA Tel: 509-371-6515


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From: microscopy.listserver-at-gmail.com
Date: Wed, 10 Jun 2020 21:07:25 -0500
Subject: [Microscopy] viaWWW: Principle of how the SAED pattern is formed

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi again
Thank you Stefan, Jim, Warren, and Robert for your help to solve this
problem. With your wise advice and the power of having a twin system I could
reliably check the suggested points and narrow the problem down to the beam
path. Indeed after cleaning the objective lens aperture (there were some
traces of oil on it) the SEM works very well already several hours now and
with a bit of luck will stay so. There is still a minute drift of stigmatism
barely noticeable but doesn't seem to be an ominous sign.
All the best
yorgos

-----Original Message-----
X-from: Yorgos Nikas [mailto:eikonika-at-otenet.gr]
Sent: Monday, June 8, 2020 08:58
To: 'microscopy-at-microscopy.com'

X-from: arunasme-at-gmail.com


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Email: arunasme-at-gmail.com Name: Arunas

Title-Subject: [Filtered] Principle of how the SAED pattern is formed

Message: Hello,

Could someone please explain to me the principle of how is a selected area diffraction pattern
formed inside the TEM column?

I understand the principles by which diffractograms from x-ray powder diffraction (XRD) are
obtained. X-ray Wavelengths, inter-planar spacings and constructive/destructive interferences.

However, I simply cannot wrap my head around the SAED pattern, how are they obtained? How is it that
its possible to get a pattern from a 2D material such a sheet of graphene? Shouldn't there be at
least two layers to generate constructive interference (hexagonal dot pattern)? I magine the beam
hitting the graphene sheet perpendicularly while the sheet is perfectly horizontal on the TEM grid.
The scatter points (atoms) would scatter the electrons in all directions equally without a
particular preference, yet we still can see intensity maxima in the shape of a hexagonal pattern.
I realize there must be a visual/logical flaw in how I imagine this process, any help to
explain/visualize the real situation would be welcome. I've gone through materials online, however
it seems the explanations lack visual representation and are very trigonometry heavy - that is not
helping me to picture the situation in my head.

Regards,

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From: microscopy.listserver-at-gmail.com
Date: Thu, 11 Jun 2020 16:44:10 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Principle of how the SAED pattern is

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Ron Anderson {randerson20-at-tampabay.rr.com}

Arunas,

With one exception, your understanding of the scattering/diffraction process is correct. The one
exception is the assumption that the thin graphene specimen on the grid is one atom layer thick. Not
true. If you are seeing a sharp, low background, hexagonal pattern on the screen the sample is at
least 10nm thick, which translates to 30 or so atomic planes thick. The atoms in each plane scatter
the electrons in all directions. All of these spheres of scattered electrons encounter electron
spheres from their neighbors with the result that most scattering directions are cancelled out. Only
those scattering directions that satisfy Bragg's Law will allow for the passage of a diffracted beam
through the specimen, which results in the observed diffraction pattern.

Ron Anderson,
Happily retired in Florida but keeping an eye of the listserver

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} Email: arunasme-at-gmail.com Name: Arunas
}
} Title-Subject: [Filtered] Principle of how the SAED pattern is formed
}
} Message: Hello,
}
} Could someone please explain to me the principle of how is a selected area diffraction pattern
} formed inside the TEM column?
}
} I understand the principles by which diffractograms from x-ray powder diffraction (XRD) are
} obtained. X-ray Wavelengths, inter-planar spacings and constructive/destructive interferences.
}
} However, I simply cannot wrap my head around the SAED pattern, how are they obtained? How is it that
} its possible to get a pattern from a 2D material such a sheet of graphene? Shouldn't there be at
} least two layers to generate constructive interference (hexagonal dot pattern)? I magine the beam
} hitting the graphene sheet perpendicularly while the sheet is perfectly horizontal on the TEM grid.
} The scatter points (atoms) would scatter the electrons in all directions equally without a
} particular preference, yet we still can see intensity maxima in the shape of a hexagonal pattern.
} I realize there must be a visual/logical flaw in how I imagine this process, any help to
} explain/visualize the real situation would be welcome. I've gone through materials online, however
} it seems the explanations lack visual representation and are very trigonometry heavy - that is not
} helping me to picture the situation in my head.
}
} Regards,
}
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From: microscopy.listserver-at-gmail.com
Date: Wed, 17 Jun 2020 17:31:06 -0500
Subject: [Microscopy] viaWWW:Amray 1700 SEM available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Colijn, Hendrik {colijn.1-at-osu.edu}

Hi,

I suspect that you are picturing X-ray diffraction in reflection. In that case, you are looking at
the planes parallel to the surface and, indeed, one plane won't give diffraction. In TEM, we are
working in transmission. Each atom becomes a scattering center. You can think of this in manner
similar to Young's slit experiment. You have 2 slits and the waves from each slit interfere to form
a diffraction pattern. In TEM, the atoms are scattering centers and act in a manner analogous to
the slits.
I hope this helps,
Henk

----------------


Hendrik O. Colijn
Center forElectronMicroscopy andAnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Wednesday, June 10,
2020 10:13 PM
To: Colijn, Hendrik {colijn.1-at-osu.edu}

X-from: Philip Kck {koeck-at-kth.se}

Hi.

I would say you should treat a 2D crystal as a phase grating.
You can find quite a lot about diffraction by phase gratings on the web and the book on Fourier
optics by Goodman also covers it.

Here's an attempt at an explanation:
If every point of the graphene sheet gives rise to a spherical wave (Huygens construction) then the
relative phases of these waves will be different depending on whether a point contains an atom or
not. You can only get constructive interference in directions where the Huygens waves are in phase.
Those directions are the diffraction spots.
In the direction of the spots the Huygens waves coming from the atoms interfere constructively with
another and so do the waves coming from the gaps between the atoms.

Does that make sense?

Philip

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Skickat: den 11 juni 2020 04:16
Till: Philip Kck
mne: [Microscopy] viaWWW: Principle of how the SAED pattern is formed

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Email: arunasme-at-gmail.com Name: Arunas

Title-Subject: [Filtered] Principle of how the SAED pattern is formed

Message: Hello,

Could someone please explain to me the principle of how is a selected area diffraction pattern
formed inside the TEM column?

I understand the principles by which diffractograms from x-ray powder diffraction (XRD) are
obtained. X-ray Wavelengths, inter-planar spacings and constructive/destructive interferences.

However, I simply cannot wrap my head around the SAED pattern, how are they obtained? How is it that
its possible to get a pattern from a 2D material such a sheet of graphene? Shouldn't there be at
least two layers to generate constructive interference (hexagonal dot pattern)? I magine the beam
hitting the graphene sheet perpendicularly while the sheet is perfectly horizontal on the TEM grid.
The scatter points (atoms) would scatter the electrons in all directions equally without a
particular preference, yet we still can see intensity maxima in the shape of a hexagonal pattern.
I realize there must be a visual/logical flaw in how I imagine this process, any help to
explain/visualize the real situation would be welcome. I've gone through materials online, however
it seems the explanations lack visual representation and are very trigonometry heavy - that is not
helping me to picture the situation in my head.

Regards,

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Title-Subject: [Filtered] Amray 1700 SEM available

Message: We have a working Amray 1700 SEM we are looking to donate. It has a tungsten filament and
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From: microscopy.listserver-at-gmail.com
Date: Wed, 17 Jun 2020 17:31:52 -0500
Subject: [Microscopy] viaWWW:JEOL 5800 or 5800LV Parts needed

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X-from: julien.debontridder-at-gmail.com

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Name: julien debontridder

Organization: retired

Title-Subject: [Filtered] JEOL 5800 or 5800LV

Message: Hello,
Our old sem JEOL 5800 broke down. The video module is broken. Does anyone happen to have a COLOR OUT
module (card) of a 5800 or a 5800LV? You can find it in front of the console of a JEOL 5800.
Thank you.
Julien DEBONTRIDDER.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 17 Jun 2020 17:36:15 -0500
Subject: [Microscopy] Fwd: SV: Fwd: Re: viaWWW: Principle of how the SAED

Contents Retrieved from Microscopy Listserver Archives
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X-from: Philip Kck {koeck-at-kth.se}

Hi.

I would say there are examples of single-layer crystals that give rise to electron diffraction.
Most membrane protein crystals are only periodic in 2 directions and they diffract very nicely.

I would say 3D-periodicity or multi-layeredness is not necessary for diffraction.
I wouldn't be surprised if a single graphene layer also gives a visible diffraction pattern.

All the best,

Philip
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Frn: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Skickat: den 11 juni 2020 23:56
Till: Philip Kck
mne: [Microscopy] Fwd: Re: viaWWW: Principle of how the SAED pattern is

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X-from: Ron Anderson {randerson20-at-tampabay.rr.com}

Arunas,

With one exception, your understanding of the scattering/diffraction process is correct. The one
exception is the assumption that the thin graphene specimen on the grid is one atom layer thick. Not
true. If you are seeing a sharp, low background, hexagonal pattern on the screen the sample is at
least 10nm thick, which translates to 30 or so atomic planes thick. The atoms in each plane scatter
the electrons in all directions. All of these spheres of scattered electrons encounter electron
spheres from their neighbors with the result that most scattering directions are cancelled out. Only
those scattering directions that satisfy Bragg's Law will allow for the passage of a diffracted beam
through the specimen, which results in the observed diffraction pattern.

Ron Anderson,
Happily retired in Florida but keeping an eye of the listserver

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} Email: arunasme-at-gmail.com Name: Arunas
}
} Title-Subject: [Filtered] Principle of how the SAED pattern is formed
}
} Message: Hello,
}
} Could someone please explain to me the principle of how is a selected area diffraction pattern
} formed inside the TEM column?
}
} I understand the principles by which diffractograms from x-ray powder diffraction (XRD) are
} obtained. X-ray Wavelengths, inter-planar spacings and constructive/destructive interferences.
}
} However, I simply cannot wrap my head around the SAED pattern, how are they obtained? How is it that
} its possible to get a pattern from a 2D material such a sheet of graphene? Shouldn't there be at
} least two layers to generate constructive interference (hexagonal dot pattern)? I magine the beam
} hitting the graphene sheet perpendicularly while the sheet is perfectly horizontal on the TEM grid.
} The scatter points (atoms) would scatter the electrons in all directions equally without a
} particular preference, yet we still can see intensity maxima in the shape of a hexagonal pattern.
} I realize there must be a visual/logical flaw in how I imagine this process, any help to
} explain/visualize the real situation would be welcome. I've gone through materials online, however
} it seems the explanations lack visual representation and are very trigonometry heavy - that is not
} helping me to picture the situation in my head.
}
} Regards,
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From: microscopy.listserver-at-gmail.com
Date: Wed, 17 Jun 2020 21:21:51 -0500
Subject: [Microscopy] Fwd: Morgagni service

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X-from: Factor, Jan {jan.factor-at-purchase.edu}


Does anybody know of a service engineer in the northeast who can provide service for an FEI Morgagni
TEM?

Please reply to jan.factor-at-purchase.edu

--Many thanks, *Jan Factor*
/**/
/*_STAY _ home. _STAY_ safe. _STOP_ the spread. _SAVE_ lives.*/
Jan Robert Factor, Ph.D.
Professor of Biology, Chair of Biology Program
Purchase College, State University of New York
Purchase, NY 10577
jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu}

*/Coral Reef Biology and Ecology Program/
*
*January in Roatan, purchase.edu/coralreef {www.purchase.edu/coralreef} *

/*Purchase College ranked in the Top 10 public liberal arts colleges nationally*/
{http://colleges.usnews.rankingsandreviews.com/best-colleges/rankings/national-liberal-arts-colleges/top-public}
*Please consider the environment before printing this e-mail*


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From: microscopy.listserver-at-gmail.com
Date: Wed, 17 Jun 2020 21:23:12 -0500
Subject: [Microscopy] viaWWW: 3DHistech P250 slide scanner

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X-from: vera.desmarais-at-einsteinmed.org

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Email: vera.desmarais-at-einsteinmed.org

Name: vera desmarais

Organization: Albert Einstein College of Medicine

Title-Subject: [Filtered] 3DHistech P250 slide scanner

Message: Today we received the renewal of our service contract for our 3DHistech p250 slide scanner
and I was shocked to see that 2020/2021 is the last year they will offer the service contract on
this instrument-eventhough the instrument is only 4 years old right now! I am used to getting at
least 10 years of service contracts on confocals and other microscopes, so I was very shocked to see
that for this scanner, 5 years is considered "end of life"....I'm just curious, does anyone have
experience with the p250 once it's beyond end of life, is it still possible to get parts and
service? I'm also interested to hear if anyone knows for how long Zeiss offers a contract on their
slide scanner?

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From: microscopy.listserver-at-gmail.com
Date: Sun, 28 Jun 2020 09:47:10 -0500
Subject: [Microscopy] viaWWW: TEM: Howie Whelan equations; accessible software

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Dear colleagues,

a lot of time has passed without dealing much with TEM and I am afraid I must recognize that my memory is failing on this one question.
I want to avoid propylene oxide as a transfer medium to Epon, so I am dehydrating biological samples with acetone and then I make mixes of acetone:Epon until 100% Epon.
I see no reason why it wouldn't work but all protocols use PO as intermediate between dehydrating agent and Epon so I feel lonesome with this idea :-)
Are there issues when mixing Epon directly with acetone without using PO?
I am quite sure that I already did in the past, some 25 years ago, but perhaps it was already a mistake at this time.

Regards,
Stephane

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From hennjohn6j-at-gmail.com Fri Jun 19 11:58:59 2020
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Email: jeffb-at-bio.umass.edu

Name: Jeffrey Blanchard

Organization: University of Massachusetts Amherst

Title-Subject: [Filtered] Sharing images

Message: We have a collection of 500 TEM images (each ~35Mb) and growing that we would like to
further analyze, annotate and share back with colleagues. To date our collaborators have made the
images available via FTP and I send them back notes. Not very satisfactory. It seems like even
FLICKR and tags would be an improvement.

} From a few searches and reading an older paper (Eliceiri et al. Biological Imaging Software Tools. Nat Methods. 2012) there are 2 platforms Bisque and OMERO that have been/are being used. Bisque has ties with the iPlant collaborative and is now hosted by Cyverse (and available for a local download). However, I am not sure this is still an active project as I had trouble uploading single images. OMERO looks great and plays well with FIJI and other tools, but seems to require a group/institution set up a server.

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From stepnoah502ybato-at-gmail.com Thu Jun 25 17:11:15 2020
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Email: pragnp-at-rpi.edu

Name: Prachi Pragnya

Organization: Rensselaer Polytechnic Institute

Title-Subject: [Filtered] TEM: Howie Whelan equations; accessible software

Message: Hello,

I am trying to understand the diffraction contrast for my in situ TEM experiments on multilayered
samples. I request guidance on any accessible software to simulate the Howie Whelan coupled
differential equations. All suggestions are welcome! Thanks in advance.

Best,
Prachi

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From: microscopy.listserver-at-gmail.com
Date: Sun, 28 Jun 2020 09:48:34 -0500
Subject: [Microscopy] viaWWW: Stage Unit Port Error- Hitachi H-7600

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Email: derrick.horne-at-botany.ubc.ca

Name: Derrick Horne

Organization: UBC BioImaging Facility

Title-Subject: [Filtered] Stage Unit Port Error- Hitachi H-7600

Message: Like many other labs, we have been shut down since mid-March due to COVID-19 and have been
trying to get ourselves upright again over the last week or so.

In so doing, we have experienced a new to us error on our Hitachi H7600.

6067: Stage unit port error


This is not a vacuum error, and it seems to be some sort of communication port problem, the effect
of which is the TEM software freezing, and the microscope console resetting. This only happens AFTER
we apply High Voltage, regardless of whether the filament voltage or beam are turned ON, or if the
sample rod is in the column. If we do not turn the HV on, then the TEM will sit happily. Sometimes
it takes long enough for the error to be generated that we get a beam, and other times it happens
during HV ramp-up or as the filament is heating up. Prior to the error, all stage controls work
through both the console and software package. After the error, neither the console controls work
(console lights go off) nor the software (all functions)

Letting it sit after the error generates a second error; 6354: Comm Unit Reset error. The software
will eventually ask to be reset, after which the console will be active again.


The first error can be recreated by unplugging the ribbon cable on port 6507 on the stage controller
unit before the HV is applied. I checked continuity (good) and resistance (all the same low value)
on the five pins, so Im calling the cable good.


Continuing conversation with our service engineers has not moved us closer to a resolution.



One last point for completeness. The last known thing done to the instrument before we started
getting this error was the installation of Chrome Remote Desktop and an antivirus program on the
Camera control computer (Win 7) which is separate from the TEM control computer (WinXP). We had
those programs removed from the computer, and double checked the log files, which shows the AVP
having performed no actions.



Does this problem sound familiar? If so, wed love to hear from you.


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From: microscopy.listserver-at-gmail.com
Date: Sun, 28 Jun 2020 09:53:41 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Sharing images

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------
X-from: V.Ray {vray-at-partbeamsystech.com}

AnnotatePro with Google Drive - does the job and priced just right as freeware.


Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
www.partbeamsystech.com
www.fibsemproducts.com
www.freudlabs.com

On 6/24/2020 8:11 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: jeffb-at-bio.umass.edu
}
} Name: Jeffrey Blanchard
}
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}
} Title-Subject: [Filtered] Sharing images
}
} Message: We have a collection of 500 TEM images (each ~35Mb) and growing that we would like to
} further analyze, annotate and share back with colleagues. To date our collaborators have made the
} images available via FTP and I send them back notes. Not very satisfactory. It seems like even
} FLICKR and tags would be an improvement.
}
} } From a few searches and reading an older paper (Eliceiri et al. Biological Imaging Software Tools. Nat Methods. 2012) there are 2 platforms Bisque and OMERO that have been/are being used. Bisque has ties with the iPlant collaborative and is now hosted by Cyverse (and available for a local download). However, I am not sure this is still an active project as I had trouble uploading single images. OMERO looks great and plays well with FIJI and other tools, but seems to require a group/institution set up a server.
}
} Any suggestions?
} Login Host: 162.245.142.25
} Listserver Email Form V - 20120416
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From: microscopy.listserver-at-gmail.com
Date: Wed, 1 Jul 2020 16:05:39 -0500
Subject: [Microscopy] viaWWW:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------

X-from: Miller, Lou A {lamiller-at-illinois.edu}


Box.com has a contract with our university, and sharing images there is great. 🤓

Sent from my iPad

} On Jun 24, 2020, at 7:19 PM, microscopy.listserver-at-gmail.com wrote:
}
} 
}
}
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} ---------------------------------------------------------------------------
}
} Email: jeffb-at-bio.umass.edu
}
} Name: Jeffrey Blanchard
}
} Organization: University of Massachusetts Amherst
}
} Title-Subject: [Filtered] Sharing images
}
} Message: We have a collection of 500 TEM images (each ~35Mb) and growing that we would like to
} further analyze, annotate and share back with colleagues. To date our collaborators have made the
} images available via FTP and I send them back notes. Not very satisfactory. It seems like even
} FLICKR and tags would be an improvement.
}
} } From a few searches and reading an older paper (Eliceiri et al. Biological Imaging Software Tools. Nat Methods. 2012) there are 2 platforms Bisque and OMERO that have been/are being used. Bisque has ties with the iPlant collaborative and is now hosted by Cyverse (and available for a local download). However, I am not sure this is still an active project as I had trouble uploading single images. OMERO looks great and plays well with FIJI and other tools, but seems to require a group/institution set up a server.
}
} Any suggestions?
} Login Host: 162.245.142.25
} Listserver Email Form V - 20120416
} ---------------------------------------------------------------------------
}
}
} ==============================Original Headers==============================
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From codyhuey981y-at-gmail.com Mon Jun 29 19:31:36 2020
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Email: joseph.mowery-at-usda.gov

Name: Joe Mowery

Organization: USDA ARS

Title-Subject: [Filtered] CMMS: Summer Speaker Series

Message: Hello everyone,
On behalf of the Chesapeake Microscopy & Microanalysis Society (CMMS), were pleased to present a
Summer Speaker Series, featuring three engaging microscopy talks in July by guest speakers Nestor
J.Zaluzec, John Shields and Bernd Zechmann.
Youre invited to join us on Zoom next week for our first virtual talk on July 9th at 3pm.

(1) July 9th at 3pm Nestor J. Zaluzec Ph.D. "Hyperspectral Imaging of Soft and Hard Matter in
the Analytical Electron Microscope: Current and Future Prospects"

(2) July 14 at 3pm John Shields Ph.D. "Challenges in preparing samples for food sciences"

(3) July 23 at 3pm Bernd Zechmann Ph.D. "Preparation of plant samples for TEM and SEM
investigations why small labs struggle to keep up with technological advances"

Please register at https://forms.gle/QYGo9GWASx7X1NX46

There will be a brief question & answer session after each talk. Attendees are encouraged to use
their webcams and microphones to encourage discussion. Hope to see you there!

Sincerely,
- Joe Mowery

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From: microscopy.listserver-at-gmail.com
Date: Wed, 1 Jul 2020 16:06:07 -0500
Subject: [Microscopy] viaWWW:

Contents Retrieved from Microscopy Listserver Archives
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Name: Reiner Bleher

Organization: Northwestern University

Title-Subject: [Filtered] Postdoc position available at Northwestern University

Message: Dear Colleagues,

a Postdoc position is available in the laboratory of Professor Vinayak Dravid at Northwestern
University (Evanston campus). The area of interest for this position is Characterizing the
Structure and Dynamics of Soft Matter with multimodal Electron Microscopy. Soft Matter here refers
to engineered proteins, megamolecules, protein complexes, MOFs and COFs. The candidate will
participate in an extensive cross-disciplinary and collaborative project across Chemistry,
Biomedical Engineering, and Cell & Molecular Biology experts at Northwestern and the University of
Chicago. The research will be conducted in the NUANCE Center (NSF Center for Facility Excellence in
the greater Midwest), which houses comprehensive sample preparation and atomic-nanoscale
characterization capabilities for biological, soft, hard, and hybrid structures. In this vibrant
environment, there is ample opportunity to learn diverse aspects of synthetic biology, simulation
and imaging of unconventional molecules/materials. We are looking for a researcher with hands-on
experience in electron microscopy, image processing, and data-processing for the reconstruction of
sample structures. The position is initially available for two years, with the potential for extension.

Interested in this unique opportunity? Please send your application with an introduction letter, a
CV, a research statement (1 page), and contact information for 3 references (name, postal & email
address, phone number) in a single PDF to tara.sadera-at-northwestern.edu and v-dravid-at-northwestern.edu.

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From: microscopy.listserver-at-gmail.com
Date: Wed, 1 Jul 2020 16:13:20 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: TEM: Howie Whelan equations; accessible

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Richard Beanland {contact-at-integrityscientific.com}

Dear Prachi,

    towards the end of last year, like you I was searching for some code to do this - and asked on
this listserver.  Although some people here were very helpful I did not find a package that I could
run easily.  My main interest is in simulating diffraction contrast images of dislocations.

I managed to find a copy of the 1973 book by Head, Humble & co. (who covered this work quite
comprehensively) in a charity shop.  This has Fortran source code.  However the version of Fortran
is so old I could hardly recognise it - and I think comments were seem as a waste of space at the
time, so it is difficult to unpick.

A couple of weeks ago I had a little time to spare so I tackled the problem myself from scratch,
with the help of the old books. You can find some python code for 2-beam simulation of dislocations
at https://github.com/rbeanland/Howie-Whelan_2-beam. This seems to do a reasonable job for
dislocations and will produce a simulation in a minute or two for most cases of interest.  There is
a trick to speed up the calculation by a factor of a hundred or so by using linear combinations of
already calculated solutions that I still need to implement, which will mean it should be possible
to calculate these images in less than a second on a modern PC.

This is very much a work in progress and I expect to carry on improving and adding to the code (I
would welcome anyone else who is interested in doing the same!)  As well as speeding the code up the
next obvious step is to add stacking faults and multiple dislocations, which would only take a
little work.  Three(+)-beam calculations would probably be the next goal, to allow weak-beam images
to be simulated.

All the best


Richard

On 28/06/2020 15:47, microscopy.listserver-at-gmail.com wrote:
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} Name: Prachi Pragnya
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} Organization: Rensselaer Polytechnic Institute
}
} Title-Subject: [Filtered] TEM: Howie Whelan equations; accessible software
}
} Message: Hello,
}
} I am trying to understand the diffraction contrast for my in situ TEM experiments on multilayered
} samples. I request guidance on any accessible software to simulate the Howie Whelan coupled
} differential equations. All suggestions are welcome! Thanks in advance.
}
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} Prachi
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From: microscopy.listserver-at-gmail.com
Date: Wed, 1 Jul 2020 16:14:35 -0500
Subject: [Microscopy] Fwd: microchemical testing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


X-from: Frank Karl {frank_karl-at-ardl.com}

I was at Ebay and noticed a microchemical test block for sale and it got me wondering. Does Cornell
Univ still teach microchemical testing? And who was Shillaber? I recognize Chamot and Mason. I
looked at the images and I'm not sure what microcghemical test I could perform with those reagents.
Any information you got would be welcome.

Stay calm...Be brave....watch for signs

Frank Karl
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305


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From: microscopy.listserver-at-gmail.com
Date: Thu, 2 Jul 2020 06:32:50 -0500
Subject: [Microscopy] Fwd: TEM examination of RBC - which blood collection tube should be

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X-from: Crameri, Sandra (AAHL, Geelong ACDP) {Sandra.Crameri-at-csiro.au}

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Hi List Server,

I am hoping for advice from anyone experienced on collection of blood for TEM analysis of red blood
cells. Please advise - is it a good idea to use blood collection tubes with anticoagulants, for
example EDTA or LiHeparin. Or is it better not to use these tubes and put the blood straight into
fixative.

The blood samples I am about to receive are from reptiles, consequently the volume of blood will be
small, only a couple of ml to play with. So any advice on general sample preparation of blood would
also be appreciated.

Many thanks in advance,

Sandy Crameri

*Sandy Crameri*
Electron Microscopy
Australian Centre for Disease Preparedness (ACDP) |CSIRO
sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au} |03 5227 5396 |0468 775 317
5 Portarlington Rd East Geelong 3220 |Private Bag 24, Geelong, VIC 3220



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From: wij.muss-at-aon.at
Date: Thu, 2 Jul 2020 08:06:16 -0500
Subject: [Microscopy] FWD: Re to 'r-bleher@northwestern.edu'; Sandra.Crameri@csiro.au, and MSA-LISTSERVER: TEM examination of RBC - which blood collection tube should be [used?]

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| FWD: Re to 'r-bleher-at-northwestern.edu'; Sandra.Crameri-at-csiro.au, and MSA-LISTSERVER: [Microscopy] TEM examination of RBC - which blood collection tube should be [used?] |
CAVE: MSA-LISTSERVER ⇒ TEXT FORMATE!

Von: MUSS Wolfgang Dr. phil./PhD (OR i.R, retired) [mailto:wij.muss-at-aon.at]
Gesendet: Donnerstag, 2. Juli 2020 15:05
An: 'r-bleher-at-northwestern.edu' {r-bleher-at-northwestern.edu} ; 'Microscopy-at-Microscopy.com' {Microscopy-at-Microscopy.com}
Betreff: Re to 'r-bleher-at-northwestern.edu'; Sandra.Crameri-at-csiro.au, and MSA-LISTSERVER: [Microscopy] TEM examination of RBC - which blood collection tube should be [used?]

Hi, dear Sandy,

would you mind to specify more in detail,

i) what the ‚expected‘ volume of „a couple of ml“ would be, and,
ii) if that – the whole collected volume of- (peripheral) blood then will be used exclusively for analysis by TEM (= buffy coat preparation) or not.

There might be solutions, but it could turn out to be/ become somehow tricky to do /perform the classical ‚buffy coat‘ – job with small volumina below – say – 4 ml.
Thanking you in advance for your kind reply,

Wolfgang







STICK IT OUT! KEEP DISTANCE – SPATIAL, NOT SOCIAL!
Keep Distance – Save a Life / perhaps Yours- Stay Safe and Well
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=====================================================
MUSS Wolfgang Dr. phil. [OR i. R.] / PhD - retired
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Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com]
Gesendet: Donnerstag, 2. Juli 2020 13:34
An: wij.muss-at-aon.at
Betreff: [Microscopy] TEM examination of RBC - which blood collection tube should be [used?]

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Hi List Server,

I am hoping for advice from anyone experienced on collection of blood for TEM analysis of red blood cells. Please advise - is it a good idea to use blood collection tubes with anticoagulants, for example EDTA or LiHeparin.
Or is it better not to use these tubes and put the blood straight into fixative.

The blood samples I am about to receive are from reptiles, consequently the volume of blood will be small, only a couple of ml to play with. So any advice on general sample preparation of blood would also be appreciated.

Many thanks in advance,

Sandy Crameri

*Sandy Crameri*
Electron Microscopy
Australian Centre for Disease Preparedness (ACDP) |CSIRO sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au} |03 5227 5396 |0468 775 317
5 Portarlington Rd East Geelong 3220 | Private Bag 24, Geelong, VIC 3220

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28, 20 -- Subject: FWD: Re to 'r-bleher-at-northwestern.edu'; Sandra.Crameri-at-csiro.au, and MSA-LISTSERVER: [Microscopy] TEM examination of RBC - which blood collection tube should be [used?]
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From: microscopy.listserver-at-gmail.com
Date: Fri, 3 Jul 2020 07:06:58 -0500
Subject: [Microscopy] Fwd: Zeiss Sigma 300 vs. FEI Quanta 600

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X-from: Dimitri Scholz {dimitri.scholz-at-ucd.ie}


Hi, has anybody got practical experience with two FEG SEMs:
Zeiss Sigma 300 and FEI Quanta 600?

I have to decide between them.
Thank you,
Dimitri



/Dimitri Scholz, PhD, Dr. Sci.
Director of Biological Imaging
Conway Institute
University College Dublin  UCD
Belfield, Dublin 4
Ireland
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Electronic mail to, from, or within the University may be the subject of a request under the Freedom
of Information Acts 1997 and 2003/

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From: microscopy.listserver-at-gmail.com
Date: Fri, 3 Jul 2020 07:07:34 -0500
Subject: [Microscopy] Fwd: Fwd: TEM examination of RBC - which blood

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X-from: Juan Carlos Leon {jcleonc-at-hotmail.com}



Dear Sandra
Pleased to greet you, I tell you that I have worked for a while with the blood of patients to
diagnose platelet diseases, what I do is obtain the blood in tubes with sodium citrate and it has
been wonderful ...
If you use tubes with EDTA, I do not think there is a problem ... however, something that has worked
for me wonderfully is separating and wash with buffer before fixing, it is important that you do
this because the large amount of proteins present in the blood makes the glutaraldehyde to
precipitate...
Greetings and I hope I can have helped ..
If you share your images I will be grateful …

*Biol. Juan Carlos León C.*
Investigador en Ciencias Médicas
Laboratorio de Microscopia Electrónica
Sección Patología Experimental
Departamento de Patología
Instituto Nacional de Ciencias Médicas y Nutrición S. Z.
Ciudad de México. MÉXICO




} Inicio del mensaje reenviado:
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} *De: *microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
} *Asunto: **[Microscopy] Fwd: TEM examination of RBC - which blood collection tube should be*
} *Fecha: *2 de julio de 2020, 6:38:29 GMT-5
} *Para: *jcleonc-at-hotmail.com {mailto:jcleonc-at-hotmail.com}
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} X-from: Crameri, Sandra (AAHL, Geelong ACDP) {Sandra.Crameri-at-csiro.au
} {mailto:Sandra.Crameri-at-csiro.au} }
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}
} Hi List Server,
}
} I am hoping for advice from anyone experienced on collection of blood for TEM analysis of red blood
} cells. Please advise - is it a good idea to use blood collection tubes with anticoagulants, for
} example EDTA or LiHeparin. Or is it better not to use these tubes and put the blood straight into
} fixative.
}
} The blood samples I am about to receive are from reptiles, consequently the volume of blood will be
} small, only a couple of ml to play with. So any advice on general sample preparation of blood would
} also be appreciated.
}
} Many thanks in advance,
}
} Sandy Crameri
}
} *Sandy Crameri*
} Electron Microscopy
} Australian Centre for Disease Preparedness (ACDP) |CSIRO
} sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au} {mailto:sandra.crameri-at-csiro.au} |03 5227
} 5396 |0468 775 317
} 5 Portarlington Rd East Geelong 3220 | Private Bag 24, Geelong, VIC 3220
}
}
}
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From: microscopy.listserver-at-gmail.com
Date: Sun, 5 Jul 2020 06:40:45 -0500
Subject: [Microscopy] Fwd: Zeiss Sigma 300 vs. FEI Quanta 600

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Leandro Lemgruber Soares {Leandro.LemgruberSoares-at-glasgow.ac.uk}


Hi Sandy

we normally put the blood straight into fixative. Later we normally have to use agar or gelatine to
keep the pellet together throughout the processing.

best
Leandro



Leandro Lemgruber, PhD FRMS

Glasgow Imaging Facility
Institute of Infection, Immunity and Inflammation
Sir Graeme Davis Building - room B621
University of Glasgow
120 University Place
Glasgow, G12 8TA UK

email: leandro.lemgrubersoares-at-glasgow.ac.uk {mailto:leandro.lemgrubersoares-at-glasgow.ac.uk}
Tel: +44 141 330 8282

Twitter: -at-iii_imaging
GIF webpage: https://www.gla.ac.uk/researchinstitutes/iii/facilities/imagingtechnologies/

The University of Glasgow, charity number SC004401

The Institute of Infection, Immunity & Inflammation encourages flexible working, so this email may
have been sent outside normal working hours. A response is not expected until you return to work.

} On 2 Jul 2020, at 12:34, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
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} X-from: Crameri, Sandra (AAHL, Geelong ACDP) {Sandra.Crameri-at-csiro.au
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} Hi List Server,
}
} I am hoping for advice from anyone experienced on collection of blood for TEM analysis of red blood
} cells. Please advise - is it a good idea to use blood collection tubes with anticoagulants, for
} example EDTA or LiHeparin. Or is it better not to use these tubes and put the blood straight into
} fixative.
}
} The blood samples I am about to receive are from reptiles, consequently the volume of blood will be
} small, only a couple of ml to play with. So any advice on general sample preparation of blood would
} also be appreciated.
}
} Many thanks in advance,
}
} Sandy Crameri
}
} *Sandy Crameri*
} Electron Microscopy
} Australian Centre for Disease Preparedness (ACDP) |CSIRO
} sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au} {mailto:sandra.crameri-at-csiro.au} |03 5227
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From slavfree818guuof-at-gmail.com Fri Jul 3 14:34:45 2020
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X-from: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

I have a Quanta FEG 250 that is now about 10 years old. It is running the older version of software
under Windows XP. I have operated a lot of other scopes from JEOL and Hitachi, but not Zeiss. I have
found the software quite usable. I have grown particularly fond of their drag over to zoom function.
I use it often and try to use it on other scopes until I realize that they are not an FEI and don't
support it.
If you have the option to get all the Navigation and stage features, please do. We have an FEI
Inspect on campus without those features and it is obviously a lessor SEM.
Warren

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, July 3,
2020 7:08 AM
To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}



X-from: Dimitri Scholz {dimitri.scholz-at-ucd.ie}


Hi, has anybody got practical experience with two FEG SEMs:
Zeiss Sigma 300 and FEI Quanta 600?

I have to decide between them.
Thank you,
Dimitri



/Dimitri Scholz, PhD, Dr. Sci.
Director of Biological Imaging
Conway Institute
University College Dublin  UCD
Belfield, Dublin 4
Ireland
Office: +353-1 716 6736
Mobile: +353-87-7961547
Web: http://conway.ucd.ie/coretech/
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Electronic mail to, from, or within the University may be the subject of a request under the Freedom
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From: microscopy.listserver-at-gmail.com
Date: Tue, 7 Jul 2020 17:24:35 -0500
Subject: [Microscopy] viaWWW: Antigen retrieval in MW posessor

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: strittl1 {strittl1-at-yahoo.com}

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X-from: Leandro Lemgruber Soares {Leandro.LemgruberSoares-at-glasgow.ac.uk}


Hi Sandy

we normally put the blood straight into fixative. Later we normally have to use agar or gelatine to
keep the pellet together throughout the processing.

best
Leandro



Leandro Lemgruber, PhD FRMS

Glasgow Imaging Facility
Institute of Infection, Immunity and Inflammation
Sir Graeme Davis Building - room B621
University of Glasgow
120 University Place
Glasgow, G12 8TA UK

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Tel: +44 141 330 8282

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The Institute of Infection, Immunity & Inflammation encourages flexible working, so this email may
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} On 2 Jul 2020, at 12:34, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
} wrote:
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} X-from: Crameri, Sandra (AAHL, Geelong ACDP) {Sandra.Crameri-at-csiro.au
} {mailto:Sandra.Crameri-at-csiro.au} }
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} Hi List Server,
}
} I am hoping for advice from anyone experienced on collection of blood for TEM analysis of red blood
} cells. Please advise - is it a good idea to use blood collection tubes with anticoagulants, for
} example EDTA or LiHeparin. Or is it better not to use these tubes and put the blood straight into
} fixative.
}
} The blood samples I am about to receive are from reptiles, consequently the volume of blood will be
} small, only a couple of ml to play with. So any advice on general sample preparation of blood would
} also be appreciated.
}
} Many thanks in advance,
}
} Sandy Crameri
}
} *Sandy Crameri*
} Electron Microscopy
} Australian Centre for Disease Preparedness (ACDP) |CSIRO
} sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au} {mailto:sandra.crameri-at-csiro.au} |03 5227
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From janiceking866aol-at-gmail.com Sun Jul 5 12:40:54 2020
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Email: randi.olsen-at-uit.no

Name: Randi Olsen

Organization: Univsersity of Tromso

Title-Subject: [Filtered] Antigen retrieval in MW posessor

Message: Hello to everyone from up north in Norway.

I have a young scientist here looking for a protocol for antigent retrieval in cryostat sections.
She has tried a lot, without finding a sucessful portocol, so therefor I would kindly as anyone that
might be able to help us out:

Do you have a protocol for antigen retrieval using a Pelco BioWave Pro system?

Best regards,
Randi Olsen
senior engeneer
Advanced microscopy core facility
UiT - The arctic University of Tromso
Norway

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From: microscopy.listserver-at-gmail.com
Date: Tue, 7 Jul 2020 17:25:39 -0500
Subject: [Microscopy] Fwd: Re: Fwd: TEM examination of RBC - which blood

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X-from: Tina Carvalho {tinacarv-at-hawaii.edu}

Hi Sandy,

I think the person who collects the green sea turtle blood I have worked on just puts it into
Trump's fixative. I will double-check. Terrestrial reptiles?

Aloha,
Tina

On Thu, Jul 2, 2020 at 4:57 AM {microscopy.listserver-at-gmail.com
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Hi List Server,

I am hoping for advice from anyone experienced on collection of blood for TEM analysis of red blood
cells. Please advise - is it a good idea to use blood collection tubes with anticoagulants, for
example EDTA or LiHeparin. Or is it better not to use these tubes and put the blood straight into
fixative.

The blood samples I am about to receive are from reptiles, consequently the volume of blood will be
small, only a couple of ml to play with. So any advice on general sample preparation of blood would
also be appreciated.

Many thanks in advance,

Sandy Crameri

*Sandy Crameri*
Electron Microscopy
Australian Centre for Disease Preparedness (ACDP) |CSIRO
sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au} } |03
5227 5396 |0468 775 317
5 Portarlington Rd East Geelong 3220 | Private Bag 24, Geelong, VIC 3220



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--
Tina Weatherby Carvalho

University of Hawaii
Pacific Biosciences Research Center
Biological Electron Microscope Facility
2538 McCarthy Mall
Honolulu, HI 96822
808-956-6251

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From: microscopy.listserver-at-gmail.com
Date: Tue, 7 Jul 2020 17:32:39 -0500
Subject: [Microscopy] viaWWW: Preparing for Virtual M&M - Webinar

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Email: anpenn-at-ncsu.edu Name: Aubrey Penn

Organization: Microscopy Society of America Student Council

Title-Subject: [Filtered] Preparing for M&M Webinar

Message: Dear Microscopists,

While we regret that the annual Microscopy & Microanalysis conference will not be held in person in
Milwaukee in August as originally planned, we are excited to let you know that MSA is planning for a
virtual conference August 3-7, and that our X-60 Pre-Meeting Congress for Students, Post-Docs, and
Early-Career Professionals will also be held virtually on August 3.

To assist meeting attendees in preparing for the virtual conference, we are hosting a webinar
entitled Preparing for M&M at 4 pm Eastern Time on Thursday, July 16. The goal of this webinar is
to prepare meeting attendees for what to expect at the virtual conference and to help them get the
most out of the experience. To that end, our panelists will answer questions about the layout of the
conference, scientific symposia, networking opportunities, vendor tutorials and booths, student
programs, and more!

This webinar will present important information for the virtual conference, as well as general
opportunities available through the M&M experience. As such, this is a great opportunity to learn
more about M&M even if youve attended the conference before.

If you are interested in attending the webinar, please register at this link:
https://docs.google.com/forms/d/e/1FAIpQLSeyjz5Ky2EvVvYJYVIDt5mR6kDJjuNXT68Yz3ohAObqcvjrqQ/viewform?usp=sf_link

Also, please be on the lookout for more information about the webinar and our panelists through
email, MSA updates, and our social media platforms.

Please consider joining us on July 16 for this webinar, and we look forward to seeing you at M&M!

Sincerely,

Jackson Spurling
Treasurer, MSA Student Council

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From: nizets2-at-yahoo.com
Date: Wed, 8 Jul 2020 02:18:40 -0500
Subject: [Microscopy] Re: viaWWW: Preparing for Virtual M&M - Webinar

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues,

Please allow me to express my excitement to be at last able for the first time in my life to attend to the M&M conference!
I've never been able to attend to it because of the distance/costs. I hope will be held virtually again in the future!
Only problem for europeans is the time lag. 
Will all sessions be available for replay on demand?
 
Best regards,
Stephane






On Wednesday, July 8, 2020, 12:47:01 AM GMT+2, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:








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Email: anpenn-at-ncsu.edu Name: Aubrey Penn

Organization: Microscopy Society of America Student Council

Title-Subject: [Filtered] Preparing for M&M Webinar

Message: Dear Microscopists,

While we regret that the annual Microscopy & Microanalysis conference will not be held in person in
Milwaukee in August as originally planned, we are excited to let you know that MSA is planning for a
virtual conference August 3-7, and that our X-60 Pre-Meeting Congress for Students, Post-Docs, and
Early-Career Professionals will also be held virtually on August 3.

To assist meeting attendees in preparing for the virtual conference, we are hosting a webinar
entitled “Preparing for M&M” at 4 pm Eastern Time on Thursday, July 16. The goal of this webinar is
to prepare meeting attendees for what to expect at the virtual conference and to help them get the
most out of the experience. To that end, our panelists will answer questions about the layout of the
conference, scientific symposia, networking opportunities, vendor tutorials and booths, student
programs, and more!

This webinar will present important information for the virtual conference, as well as general
opportunities available through the M&M experience. As such, this is a great opportunity to learn
more about M&M — even if you’ve attended the conference before.

If you are interested in attending the webinar, please register at this link:
https://docs.google.com/forms/d/e/1FAIpQLSeyjz5Ky2EvVvYJYVIDt5mR6kDJjuNXT68Yz3ohAObqcvjrqQ/viewform?usp=sf_link

Also, please be on the lookout for more information about the webinar and our panelists through
email, MSA updates, and our social media platforms.

Please consider joining us on July 16 for this webinar, and we look forward to seeing you at M&M!

Sincerely,

Jackson Spurling
Treasurer, MSA Student Council

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34, 38 -- Date: Wed, 8 Jul 2020 07:24:23 +0000 (UTC)
34, 38 -- From: Stephane Nizet {nizets2-at-yahoo.com}
34, 38 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} ,
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From: microscopy.listserver-at-gmail.com
Date: Wed, 8 Jul 2020 07:21:30 -0500
Subject: [Microscopy] Fwd: [Filtered] Re: viaWWW: Preparing for Virtual MM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Bullitt, Esther {bullitt-at-bu.edu}

Status:
Dear Stephane and EM community,

We, too, are excited about the possibilities opened up by having a virtual M&M meeting!
The registration fee for students and postdocs is only $20
For members, $75, and non-members, $150.

All of the platform talks and posters will be available on demand for the entire length of the
meeting. At scheduled times the presentations will be streamed, and presenters will be available
for live text chats. — Presenters have the option of having their content for an extra
month, if they wish.

Some events will be live-streamed, including the plenary session, live chats with exhibitors,
and some social gatherings.

The week-at-a-glance calendar for the meeting will be coming out within the next few days,
so keep an eye out for that.

See you there!

Sincerely,
Esther

Esther Bullitt, Ph.D.
President 2020, Microscopy Society of America


} On Jul 8, 2020, at 3:26 AM, nizets2-at-yahoo.com wrote:
}
}
}
}
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}
} Dear colleagues,
}
} Please allow me to express my excitement to be at last able for the first time in my life to attend to the M&M conference!
} I've never been able to attend to it because of the distance/costs. I hope will be held virtually again in the future!
} Only problem for europeans is the time lag.
} Will all sessions be available for replay on demand?
}
} Best regards,
} Stephane
}
}
}
}
}
}
} On Wednesday, July 8, 2020, 12:47:01 AM GMT+2, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:
}
}
}
}
}
}
}
}
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} using the WWW based Form at http://microscopy.com/MLFormMail.html
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} benefit from our collective wisdom
} ---------------------------------------------------------------------------
}
} Email: anpenn-at-ncsu.edu Name: Aubrey Penn
}
} Organization: Microscopy Society of America Student Council
}
} Title-Subject: [Filtered] Preparing for M&M Webinar
}
} Message: Dear Microscopists,
}
} While we regret that the annual Microscopy & Microanalysis conference will not be held in person in
} Milwaukee in August as originally planned, we are excited to let you know that MSA is planning for a
} virtual conference August 3-7, and that our X-60 Pre-Meeting Congress for Students, Post-Docs, and
} Early-Career Professionals will also be held virtually on August 3.
}
} To assist meeting attendees in preparing for the virtual conference, we are hosting a webinar
} entitled “Preparing for M&M” at 4 pm Eastern Time on Thursday, July 16. The goal of this webinar is
} to prepare meeting attendees for what to expect at the virtual conference and to help them get the
} most out of the experience. To that end, our panelists will answer questions about the layout of the
} conference, scientific symposia, networking opportunities, vendor tutorials and booths, student
} programs, and more!
}
} This webinar will present important information for the virtual conference, as well as general
} opportunities available through the M&M experience. As such, this is a great opportunity to learn
} more about M&M — even if you’ve attended the conference before.
}
} If you are interested in attending the webinar, please register at this link:
} https://docs.google.com/forms/d/e/1FAIpQLSeyjz5Ky2EvVvYJYVIDt5mR6kDJjuNXT68Yz3ohAObqcvjrqQ/viewform?usp=sf_link
}
} Also, please be on the lookout for more information about the webinar and our panelists through
} email, MSA updates, and our social media platforms.
}
} Please consider joining us on July 16 for this webinar, and we look forward to seeing you at M&M!
}
} Sincerely,
}
} Jackson Spurling
} Treasurer, MSA Student Council
}
} Login Host: 107.13.165.234
} Listserver Email Form V - 20120416
} ---------------------------------------------------------------------------
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}
} ==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Thu, 9 Jul 2020 07:04:20 -0500
Subject: [Microscopy] viaWWW: SEM filaments available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Join us for the webinar series on X-ray Computed Tomography for Materials Science & Life Science to find out how X-ray CT can help your research.

When: July 15, 2020 11:00 AM Pacific Daylight Saving Time

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Email: jpshield-at-uga.edu

Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] SEM filaments

Message: I have two unopened boxes of "standard loop filaments" for "AEI, Cambridge S Series,
Novascan, Semco and Nanolab"(EMS Cat# 81020). Our Zeiss 1450 SEM is no longer with us and so we no
longer need them.

If you can use the filaments, I will send them (USA shipping only).

Gracias,
John Shields
Georgia Electron Microscopy
UGA Athens, GA

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From: microscopy.listserver-at-gmail.com
Date: Thu, 9 Jul 2020 07:13:10 -0500
Subject: [Microscopy] viaWWW: Antigen retrieval in MW posessor

Contents Retrieved from Microscopy Listserver Archives
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X-from: Wendy Salmon {wsalmon-at-wi.mit.edu}



Hi Randi,
Could you provide information on what type of tissue it is?
I don't have a lot of personal experience, but one of my users has found that an InstantPot (kitchen
pressure cooker) works best for her liver and skin sections.
Wishing you peace and good health,

Wendy

--------------------
Wendy C Salmon, M.A.
Manager, W.M. Keck Facility for Biological Imaging
/Limited availability before 1pm M,W,F due to 2-year-old side-kick/

Whitehead Institute for Biomedical Research
455 Main St., Rm 447
Cambridge, MA 02142 USA
e: wsalmon-at-wi.mit.edu {mailto:wsalmon-at-wi.mit.edu}
w: microscopy.wi.mit.edu {http://microscopy.wi.mit.edu}
she/her/hers
{http://microscopy.wi.mit.edu}




On Wed, Jul 8, 2020 at 12:39 AM {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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Email: randi.olsen-at-uit.no {mailto:randi.olsen-at-uit.no}

Name: Randi Olsen

Organization: Univsersity of Tromso

Title-Subject: [Filtered] Antigen retrieval in MW posessor

Message: Hello to everyone from up north in Norway.

I have a young scientist here looking for a protocol for antigent retrieval in cryostat sections.
She has tried a lot, without finding a sucessful portocol, so therefor I would kindly as anyone
that
might be able to help us out:

Do you have a protocol for antigen retrieval using a Pelco BioWave Pro system?

Best regards,
Randi Olsen
senior engeneer
Advanced microscopy core facility
UiT - The arctic University of Tromso
Norway

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From: microscopy.listserver-at-gmail.com
Date: Thu, 9 Jul 2020 07:14:09 -0500
Subject: [Microscopy] Fwd: AO Microtome Model 880AO Microtome Model 880 instruction Manual

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


X-from: Frank Karl {frank_karl-at-ardl.com}

I'm looking for an instruction manual for a AO Microtome Model 880. This is a manual. Clamp to
table microtome. I've been interested in cutting TS of wood but really like the instructions. Does
anyone have a e-copy?




Stay calm…Be brave….Watch for signs

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305


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From: microscopy.listserver-at-gmail.com
Date: Thu, 9 Jul 2020 07:14:54 -0500
Subject: [Microscopy] Fwd: Microscopy & Microanalysis 2020 meeting schedule is online now!

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


X-from: Bullitt, Esther {bullitt-at-bu.edu}


The virtual M&M 2020  schedule is out now! https://www.microscopy.org/MandM/2020/MM2020_schedule.pdf

The meeting website is: https://www.microscopy.org/MandM/2020/

And to register for 'Preparing for M&M webinar’ presented by the Microscopy Society Student Counci
on July 16 at 4,
Go to:
https://docs.google.com/forms/d/e/1FAIpQLSeyjz5Ky2EvVvYJYVIDt5mR6kDJjuNXT68Yz3ohAObqcvjrqQ/viewform?usp=sf_link


See you there!

Sincerely,
Esther

Esther Bullitt, Ph.D.
President, MSA

} On Jul 8, 2020, at 8:30 AM, microscopy.listserver-at-gmail.com
} {mailto:microscopy.listserver-at-gmail.com} wrote:
}
}
}
}
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} X-from: Bullitt, Esther {bullitt-at-bu.edu {mailto:bullitt-at-bu.edu} }
}
} Status:
} Dear Stephane and EM community,
}
} We, too, are excited about the possibilities opened up by having a virtual M&M meeting!
} The registration fee for students and postdocs is only $20
} For members, $75, and non-members, $150.
}
} All of the platform talks and posters will be available on demand for the entire length of the
} meeting.  At scheduled times the presentations will be streamed, and presenters will be available
} for live text chats. — Presenters have the option of having their content for an extra
} month, if they wish.
}
} Some events will be live-streamed, including the plenary session, live chats with exhibitors,
} and some social gatherings.
}
} The week-at-a-glance calendar for the meeting will be coming out within the next few days,
} so keep an eye out for that.
}
} See you there!
}
} Sincerely,
} Esther
} —
} Esther Bullitt, Ph.D.
} President 2020, Microscopy Society of America
}
}
} } On Jul 8, 2020, at 3:26 AM, nizets2-at-yahoo.com {mailto:nizets2-at-yahoo.com} wrote:
} }
} }
} }
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} } Dear colleagues,
} }
} } Please allow me to express my excitement to be at last able for the first time in my life to
} } attend to the M&M conference!
} } I've never been able to attend to it because of the distance/costs. I hope will be held virtually
} } again in the future!
} } Only problem for europeans is the time lag.
} } Will all sessions be available for replay on demand?
} }
} } Best regards,
} } Stephane
} }
} }
} }
} }
} }
} }
} } On Wednesday, July 8, 2020, 12:47:01 AM GMT+2, microscopy.listserver-at-gmail.com
} } {mailto:microscopy.listserver-at-gmail.com} {microscopy.listserver-at-gmail.com
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} } X-from: anpenn-at-ncsu.edu {mailto:anpenn-at-ncsu.edu}
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} } Email: anpenn-at-ncsu.edu {mailto:anpenn-at-ncsu.edu} Name: Aubrey Penn
} }
} } Organization: Microscopy Society of America Student Council
} }
} } Title-Subject: [Filtered] Preparing for M&M Webinar
} }
} } Message: Dear Microscopists,
} }
} } While we regret that the annual Microscopy & Microanalysis conference will not be held in person in
} } Milwaukee in August as originally planned, we are excited to let you know that MSA is planning for a
} } virtual conference August 3-7, and that our X-60 Pre-Meeting Congress for Students, Post-Docs, and
} } Early-Career Professionals will also be held virtually on August 3.
} }
} } To assist meeting attendees in preparing for the virtual conference, we are hosting a webinar
} } entitled “Preparing for M&M” at 4 pm Eastern Time on Thursday, July 16. The goal of this webinar is
} } to prepare meeting attendees for what to expect at the virtual conference and to help them get the
} } most out of the experience. To that end, our panelists will answer questions about the layout of the
} } conference, scientific symposia, networking opportunities, vendor tutorials and booths, student
} } programs, and more!
} }
} } This webinar will present important information for the virtual conference, as well as general
} } opportunities available through the M&M experience. As such, this is a great opportunity to learn
} } more about M&M — even if you’ve attended the conference before.
} }
} } If you are interested in attending the webinar, please register at this link:
} } https://docs.google.com/forms/d/e/1FAIpQLSeyjz5Ky2EvVvYJYVIDt5mR6kDJjuNXT68Yz3ohAObqcvjrqQ/viewform?usp=sf_link
} }
} } Also, please be on the lookout for more information about the webinar and our panelists through
} } email, MSA updates, and our social media platforms.
} }
} } Please consider joining us on July 16 for this webinar, and we look forward to seeing you at M&M!
} }
} } Sincerely,
} }
} } Jackson Spurling
} } Treasurer, MSA Student Council
} }
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From: microscopy.listserver-at-gmail.com
Date: Sat, 11 Jul 2020 07:54:52 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Antigen retrieval in MW posessor

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X-from: Jerome Jasso {jfjasso493-at-gmail.com}

Hello Randy,

There are a couple of protocols at the TPI website using the Pelco BioWave system.  A buffer of
choice would be EDTA, although you can try several other buffers depending upon the biomarkers you
are using.  Make sure you are using fresh charged slides for your sections to avoid lifting or trapping.

Kindest regards,
Jerry Jasso, Regional Manager
Element Pi
jerry-at-elementpi.com {mailto:jerry-at-elementpi.com}
(330) 319-4236

On Thu, Jul 9, 2020 at 8:34 AM {microscopy.listserver-at-gmail.com
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X-from:         Wendy Salmon {wsalmon-at-wi.mit.edu {mailto:wsalmon-at-wi.mit.edu} }



Hi Randi,
Could you provide information on what type of tissue it is?
I don't have a lot of personal experience, but one of my users has found that an InstantPot
(kitchen
pressure cooker) works best for her liver and skin sections.
Wishing you peace and good health,

Wendy

--------------------
Wendy C Salmon, M.A.
Manager, W.M. Keck Facility for Biological Imaging
/Limited availability before 1pm M,W,F due to 2-year-old side-kick/

Whitehead Institute for Biomedical Research
455 Main St., Rm 447
Cambridge, MA 02142 USA
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     Name: Randi Olsen

     Organization: Univsersity of Tromso

     Title-Subject: [Filtered] Antigen retrieval in MW posessor

     Message: Hello to everyone from up north in Norway.

     I have a young scientist here looking for a protocol for antigent retrieval in cryostat
sections.
     She has tried a lot, without finding a sucessful portocol, so therefor I would kindly as
anyone
     that
     might be able to help us out:

     Do you have a protocol for antigen retrieval using a Pelco BioWave Pro system?

     Best regards,
     Randi Olsen
     senior engeneer
     Advanced microscopy core facility
     UiT - The arctic University of Tromso
     Norway

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--
/Best regards,/
*/Jerome (Jerry) Jasso/*
/Home: (616) 887-1994/
/Cell: (330) 319-4236/
/jfjasso493-at-gmail.com {mailto:jfjasso493-at-gmail.com} /

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From: microscopy.listserver-at-gmail.com
Date: Sat, 11 Jul 2020 08:23:04 -0500
Subject: [Microscopy] viaWWW: TEM H-9000NAR trouble

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Email: eric.gautron-at-cnrs-imn.fr

Name: Eric

Organization: CNRS

Title-Subject: [Filtered] TEM H-9000NAR trouble

Message: Dear all,
I have this problem with my old H-9000 NAR : the sample position display indicates E-2 on X, and
there is no other indication on Y, Tilt and azimuth. I do not remember what it coresponds to. Any
idea to solve that ?
Best regards,
Eric

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From: microscopy.listserver-at-gmail.com
Date: Tue, 14 Jul 2020 16:48:02 -0500
Subject: [Microscopy] viaWWW: SEM filaments blowing

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Name: John Shields

Organization: University of Georgia

Title-Subject: [Filtered] Filaments gone!

Message: Hi all!
The aforementioned SEM filaments have already been spoken for. Thanks!
John S
Georgia Electron Microscopy

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From 111-at-agrizaturini.com.com Sat Jul 11 22:27:04 2020
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Email: nyilmaz-at-mersin.edu.tr

Name: sakir necat yilmaz

Organization: University of Mersin

Title-Subject: [Filtered] Megaview III camera problem

Message: Hi All,

We're using a Megaview III camera installed on Jeol JEM 1011 microscope. We've an air leakage
problem from o-rings of camera prism bars. Does anybody knows the sizes and/or part number of these
o-rings on that bars.
Cheers...

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Email: ravi.thakkar369-at-gmail.com

Name: Ravi

Title-Subject: [Filtered] SEM filament blown.

Message: Hi,
I am using Hitachi S3500-N SEM, it runs on tungsten filament. The filament is getting blown as I
start the HV, it shows 0 current. I lost of three brand new filaments in a row. Can anyone help me
how to troubleshoot this?

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From: crwinkler-at-ncsu.edu
Date: Tue, 14 Jul 2020 18:56:22 -0500
Subject: [Microscopy] JEOL 2000FX and parts for donation to non-profit orgs

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

We are decommissioning our vintage JEOL 2000FX system and all
associated accessories--chiller, UPS, sample holders, and spare parts
separate from the instrument itself. The system is in working order
though many of the attachments are no longer functional, including the
EDS system and STEM scan generator and detectors. Basically, it's
useful as a 80-200kV TEM. A Gatan bottom mount Orius camera is
attached and functional.

The system will be sent to surplus in 30 days or less. If you're
interested in taking the complete system, or just want the
holders/spare parts, please contact me ASAP. We cannot cover the
expense of moving the system nor shipping of any parts. We also cannot
cannibalize the microscope column for parts. Most importantly, since
we are a non-profit organization, we can only donate to other
non-profits; we cannot sell or give away the system and/or accessories
to any for-profit organizations.

You can email or call me directly to discuss.

Thanks,
Chris
--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
https://www.aif.ncsu.edu/
Cell: 267-496-0587

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From: microscopy.listserver-at-gmail.com
Date: Wed, 15 Jul 2020 06:24:44 -0500
Subject: [Microscopy] viaWWW: SEM filaments blowing

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Yorgos Nikas {eikonika-at-otenet.gr}

Hi Ravi
We had exactly the same problem with a Jeol JSM5600 and we found that the
high tension control board had a transistor blown, we changed it and then
was OK.
That's all I know and wish you good luck in your diagnosis -then the
treatment is simple..
Best regards
Yorgos

Dr Yorgos Nikas
Athens Innovative Microscopy
Skra 36 Voula 16673 GREECE

Tel/fax +30 210 8957677
mobile +30 6945 107477
www.eikonika.netwww.aim.cat
*************************************


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Name: Ravi

Title-Subject: [Filtered] SEM filament blown.

Message: Hi,
I am using Hitachi S3500-N SEM, it runs on tungsten filament. The filament
is getting blown as I start the HV, it shows 0 current. I lost of three
brand new filaments in a row. Can anyone help me how to troubleshoot this?

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From: microscopy.listserver-at-gmail.com
Date: Wed, 15 Jul 2020 06:26:20 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: SEM filaments blowing

Contents Retrieved from Microscopy Listserver Archives
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X-from: Crane, Dan - OSHA {Crane.Dan-at-dol.gov}

Initial comments:

Rebuilt? (uncommon today, but not unheard-of) Solution - use new filaments, not rebuilts

Pre -centered? Solution - do not trust that they are really pre-centered and make sure and
critically center them if necessary.

Make sure that you are setting the correct height in the Wehnelt cap. Do not trust that the height
did not change when you took it off. The manual tell you how to properly do this. The depth of the
filament in the aperture will determine how much current it takes to cause emission and may cause
premature failure.

Important! Critically clean the Wehnelt aperture along with the whole anode cap and cathode cap
every time (shiny caps). It is easy to get "in a hurry" and just change the filament. (Also, I know
that you know this, but do not handle any of the components with bare fingers.)

Let the chamber pump down extra time after a filament exchange to allow for any potential outgassing
in the gun section.

I have had a few bad batches of filaments that have uneven heights and were just poorly made.

It is possible for the current control to be set way too high, or the current control circuit may
have failed. While it is a great work horse SEM, the Hitachi S3500-N is getting a little long in the
tooth.

Dan Crane
(Long time with S3500-N)


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, July 14, 2020
3:51 PM
To: Crane, Dan - OSHA {Crane.Dan-at-dol.gov}

X-from: Regan M {reganhll-at-gmail.com}

Hi,
there might be 3 causes.

1. Poor vacuum. Please check ur vacuum gauges before starting the HV. If the gauges are faulty and
you open the HV leading to burn out of the filaments. Check the tip using a Light microscope based
on how the burn filaments ends appear you could guess the cause of the issue.

2. What is the age of tungsten filaments and from which batch they are. If it so happens that they
are all from the same batch it could be that the batch is faulty itself. Test it out with other
batch filaments. Supplier must have this data from other users of these filaments.

3. Check your voltage board electronics, There could be a surge which might lead to overload.

Hope it helps,
Greetings,



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Name: Ravi

Title-Subject: [Filtered] SEM filament blown.

Message: Hi,
I am using Hitachi S3500-N SEM, it runs on tungsten filament. The filament is getting blown as I
start the HV, it shows 0 current. I lost of three brand new filaments in a row. Can anyone help me
how to troubleshoot this?

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From: microscopy.listserver-at-gmail.com
Date: Wed, 15 Jul 2020 06:27:00 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: SEM filaments blowing

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X-from: Bil Schneider {wfschneider-at-wisc.edu}

Ravi,
I’m inclined to think something is grounding at the grid-cap or anode. Is the grid cap touching the
filament tip? How many spacers are going in to the base before screwing on the Wehnalt? Is the
anode/grid cap discolored, dirty? How long had your filaments been lasting before this event?

Bil Schneider SEM Lab Manager
UW Madison Geosciences 608-333-7874



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Title-Subject: [Filtered] SEM filament blown.

Message: Hi,
I am using Hitachi S3500-N SEM, it runs on tungsten filament. The filament is getting blown as I
start the HV, it shows 0 current. I lost of three brand new filaments in a row. Can anyone help me
how to troubleshoot this?

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From: gary_laevsky-at-yahoo.com
Date: Wed, 15 Jul 2020 06:45:59 -0500
Subject: [Microscopy] Imaging Specialist needed in Molecular Biology at Princeton

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

A full-time Research Specialist position is available in the Confocal Imaging Facility (CIF) laboratory in the Department of Molecular Biology at Princeton University. The CIF provides support for Princeton University faculty and student research utilizing multiple imaging modalities. This position reports directly to the Facility Director.  This is a 2-year term position with the possibility of renewal.




Requisition # 2020-11822

https://research-princeton.icims.com/jobs/11822

Responsibilities of the Confocal Imaging Facility Research Specialist include:

 

• Train and assist with the execution of complex and routine widefield and confocal experiments with users (students, post-docs and faculty). Orient new users to the lab and directly contribute to the successful daily operation of the facility.

 

• Consult with users (students, post-docs and faculty) concerning assay development and appropriate instrument application/optimization.

 

• Perform daily and weekly quality control checks on all microscopes. Keep accurate, legible records to monitor instrument performance. In concert with Facility Director, complete scheduled and unscheduled system maintenance as needed.

 

• Assist in maintaining instrument/experiment calendars and become expert in the use of iLab scheduling software.


• Archive all facility data and maintain workstation computers.

 

• Other duties as assigned by the CIF Director.

 

Qualifications:

Master's degree in a scientific discipline and 4-6 years previous experience  in a laboratory experience are required.  
Previous imaging/confocal knowledge and hands-on experience with routine and advanced multi-dimensional experiments is required. 
The candidate should have: strong interpersonal and communication skills, both written and verbal; independent critical thinking and problem solving ability; flexibility; and excellent attention to detail.
Experience in a fast-paced, multi-tasking environment and the ability to work independently is required.
Advanced knowledge of Microsoft suite (Word, Excel, PowerPoint) software.

 

 

 

Princeton University is an Equal Opportunity/Affirmative Action Employer and all qualified applicants will receive consideration for employment without regard to age, race, color, religion, sex, sexual orientation, gender identity or expression, national origin, disability status, protected veteran status, or any other characteristic protected by law. EEO IS THE LAW



--
Best,


Gary Laevsky, Ph.D.
Director, Confocal Imaging Facility
Nikon Center of Excellence
Co-Founder, North Atlantic Microscopy Society (NAMS) 
https://namsmicroscopy.com/
Dept. of Molecular Biology
Washington Rd.
Princeton University 
Princeton, New Jersey, 08544-1014
(O) 609 258 5432
(C) 508 507 1310


Be safe and be well.


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From: microscopy.listserver-at-gmail.com
Date: Fri, 24 Jul 2020 06:30:20 -0500
Subject: [Microscopy] viaWWW:Water sensor Hitachi HS-8 TEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




X-from: Adam McCombs {adam-at-mccombs.us}



Can you post a photo of what the burnt out filament looks like somewhere, preferably under an
inspection scope? In some cases it can be very helpful for diagnostics.



Comment added by your Friendly Neighborhood SysOp

Please remember that if you try to post attachements/images to
the microscopylistserver they will be rejected. The photo
suggested above is fine, but it should be on a public viewing site
and just include the URL/link to the image.

Cheers, - Nestor




On Tue, Jul 14, 2020 at 2:55 PM {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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Email: ravi.thakkar369-at-gmail.com {mailto:ravi.thakkar369-at-gmail.com}

Name: Ravi

Title-Subject: [Filtered] SEM filament blown.

Message: Hi,
I am using Hitachi S3500-N SEM, it runs on tungsten filament. The filament is getting blown as I
start the HV, it shows 0 current. I lost of three brand new filaments in a row. Can anyone help me
how to troubleshoot this?

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From smithdiana157ma-at-gmail.com Thu Jul 16 13:05:54 2020
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X-from: Erico Freitas {ericotadeu-at-ufmg.br}

Hi Ravi,

I would suggest you the same as Dan Crane already said.
We have had a similar issue with our W-TEM. A perfect Wehnelt cleaning procedure helped us overcome
that. We also vacuum cleaned the gun assembly with a dedicated vacuum cleaner for that with a clean
tip (you better not touch the gun assembly with the vacuum cleaner tip, even if that was nicely
cleaned up).

Wish you success
Regards,
Erico

Em qua., 15 de jul. de 2020 às 08:41, {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } escreveu:




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X-from: Bil Schneider {wfschneider-at-wisc.edu {mailto:wfschneider-at-wisc.edu} }

Ravi,
I’m inclined to think something is grounding at the grid-cap or anode. Is the grid cap touching the
filament tip? How many spacers are going in to the base before screwing on the Wehnalt? Is the
anode/grid cap discolored, dirty? How long had your filaments been lasting before this event?

Bil Schneider SEM Lab Manager
UW Madison Geosciences 608-333-7874



On Jul 14, 2020, at 4:58 PM, microscopy.listserver-at-gmail.com
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Name: Ravi

Title-Subject: [Filtered] SEM filament blown.

Message: Hi,
I am using Hitachi S3500-N SEM, it runs on tungsten filament. The filament is getting blown as I
start the HV, it shows 0 current. I lost of three brand new filaments in a row. Can anyone help me
how to troubleshoot this?

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--
Erico Freitas

Physicist / Microscopist / Lab Manager
Transmission Electron Microscopy for Materials Science laboratory
Center of Microscopy
Universidade Federal de Minas Gerais (UFMG)
Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901.
+55-31-3409-7573
+55-31-3409-7575

CV Lattes: *http://lattes.cnpq.br/8786127123101199*
{https://wwws.cnpq.br/cvlattesweb/PKG_MENU.menu?f_cod=DE6B009EAB5F41052FDE9CDAAECDEB36#}

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From janiceking866j-at-gmail.com Fri Jul 17 02:07:28 2020
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Dear colleagues,

We have an opening this fall for a postdoctoral research associate, with a particular focus on analytical STEM and data analytics of complex oxides and quantum materials. A description for the position is given below.

If you’d like to learn more about our work and apply, please visit: https://www.stevenspurgeon.com/news/postdoctoral-researcher-position-in-electron-microscopy-and-data-analytics-of-complexoxides

Position Title: Post Doc Research Associate - Electron Microscopy and Data Analytics of Complex Oxides
 
Location: Pacific Northwest National Laboratory, Richland, Washington, United States
 
Job Description
 
Contribute to PNNL’s goals in our Chemical Dynamics Initiative to study defects in complex oxides using scanning transmission electron microscopy. As a materials scientist in the Materials Characterization team, you will join a talented, multi-investigator team to investigate the evolution of epitaxial complex oxide thin films and heterostructures in extreme environments. You are expected to contribute significantly to a new project with the goal to understand the atomic-scale structure, chemistry, and properties of these materials using advanced electron microscopy and data analytics. You will also examine emerging quantum phenomena in complex materials systems and grow our correlative atomic-scale characterization capabilities. You will have access to a suite of world-class research instrumentation, including multiple aberration-corrected microscopes and high-performance computing capabilities, as well as the Environmental Molecular Sciences Laboratory (EMSL), a unique DOE User Facility on the PNNL campus.

 
The Ideal Candidate
 
If you are a highly motivated researcher ready to test your talents and training in Physics, Materials, or Chemistry and hone your skills at a national laboratory widely recognized for its work in the physical sciences, we want to connect with you. Details are below; you do not need to meet all of the preferred qualifications to be considered.
 
What you will do:
 
• Conduct independent research and work on team assignments
 
• Lead manuscript development and maintain a strong overall publication record in the peer-reviewed scientific literature
 
• Interact, communicate, and problem solve with a diverse team of research staff
 
• Present research at technical conferences and project/program review meetings
 
• Participate in the development of research proposals
 
Minimum Qualifications
 
• Candidates must have received a PhD within the past five years (60 months) or within the next 8 months from an accredited college or university.
 
Preferred Qualifications
 
• In-depth knowledge, practical experience, and a strong publication record in complex oxide thin film characterization. Previous research experience on understanding structure-property relationships in precisely designed heteroepitaxial thin films and devices is highly preferred.
 
• Hands-on experience in aberration-corrected scanning transmission electron microscopy (STEM), electron energy loss spectroscopy (EELS), and energy-dispersive X-ray spectroscopy (EDS).
 
• Hands-on experience in atomic-resolution imaging and spectroscopy of complex oxide and semiconductor materials.
 
• Experience in multislice image simulation codes (µSTEM, PRISMATIC, QSTEM, etc.) and their integration with atomistic simulations.
 
• Knowledge of data analytics and image processing to quantify electron microscopy imaging and spectral data sets.
 
• Experience in focused ion beam (FIB) sample preparation.
 
• Familiarity with atom probe tomography (APT) and correlative STEM-APT analysis methods.
 
• The ability to adapt, refine, or innovate experimental tools based on research needs.
 
• Ability to work independently and take initiative in the completion of tasks important to the projects. These include preparation of first drafts of papers for peer-reviewed journals, technical presentations at scientific conferences.
 
• Ability to work in collaboration with a diverse group of scientists and technical staff, ability to communicate effectively with experimentalist and computational modeling colleagues.
 
• Previous postdoc experience is highly preferred.
 
• Strong verbal and written communications skills.
 
• Ph.D. in Materials Sciences, Physics, Physical Chemistry, or related field
 
Equal Employment Opportunity
 
Battelle Memorial Institute (BMI) at Pacific Northwest National Laboratory (PNNL) is an Affirmative Action/Equal Opportunity Employer and supports diversity in the workplace. All employment decisions are made without regard to race, color, religion, sex, national origin, age, disability, veteran status, marital or family status, sexual orientation, gender identity, or genetic information. All BMI staff must be able to demonstrate the legal right to work in the United States. BMI is an E-Verify employer. Learn more at jobs.pnnl.gov.
 
Please be aware that the Department of Energy (DOE) prohibits DOE employees and contractors from participation in certain foreign government talent recruitment programs. If you are offered a position at PNNL and are currently a participant in a foreign government talent recruitment program you will be required to disclose this information before your first day of employment.
 
Other Information
 
• Radiological worker-I & II training will be required for this position, since some of our instrumentation is located in radiologically controlled spaces, but no work with radiological samples will be conducted.
 
• The work requires familiarity with chemical and radiological hazards.

______________________________________
Steven R. Spurgeon, Ph.D.
Research Scientist
Energy and Environment Directorate

Pacific Northwest National Laboratory
902 Battelle Boulevard
P.O. Box 999 MSIN:P7-25
Richland, WA 99352

Tel: +1-509-371-7709



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9, 90 -- Subject: Open postdoctoral position in STEM and data analytics of complex
9, 90 -- oxides -at- PNNL
9, 90 -- Thread-Topic: Open postdoctoral position in STEM and data analytics of complex
9, 90 -- oxides -at- PNNL
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From janiceking866qejwo-at-gmail.com Wed Jul 22 20:53:20 2020
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Email: becks-at-ncc.edu Name: Steve Beck

Organization: Nassau Community College

Title-Subject: [Filtered] Water sensor

Message: Dear Colleagues,

I have an old Hitachi HS-8 TEM that is having a start up problem. Last year I had to troubleshoot
the vacuum power supply module. Now, I cannot get even a low vacuum gauge movement. I noticed the
diffusion pump is not heating. There seems to be good flow from my water chiller, however, I am used
to hearing a microswitch click when turning the water on and off. Could it be a defective water flow
sensor? Are there replacements available for such an old TEM!

Thanks for any suggestions!

Steve
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From: microscopy.listserver-at-gmail.com
Date: Fri, 24 Jul 2020 16:14:38 -0500
Subject: [Microscopy] Fwd: Link EDX counts fluctuating down to zero rate for a few seconds

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X-from: Sylvain Poudrette {spoudrette-at-videotron.ca}

Hello

Problem: EDX counts fluctuating down to zero rate for a few seconds
Anyone experienced this before ?

The beam current measured at stage with A Keithley meter and illumination from SE are very stable.
So I suspect this LN2 EDX is going to need repair. I just had it pumped down to a very good vacuum
level hence rid of any ice.

Sylvain

Private owner / hobbyist with an Amray 1850
& Isis EDX

Sent from my iPhone

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From: microscopy.listserver-at-gmail.com
Date: Sun, 26 Jul 2020 10:19:57 -0500
Subject: [Microscopy] viaWWW: SEM Filament Vibration

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Email: miketoalson-at-gmail.com Name: Mike Toalson

Organization: Element Pi

Title-Subject: [Filtered] SEM Filament Vibration

Message: Looking for feedback from the EM community. It was recently suggested to me that what
appears to be wave like image distortion normally attributed to EMI could actually be induced by
vibration of the Tungsten filament that results from incorrect centering or height adjustment in
relation to the Wehnelt.

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From: microscopy.listserver-at-gmail.com
Date: Sun, 26 Jul 2020 10:38:28 -0500
Subject: [Microscopy] Fwd: Link EDX counts fluctuating down to zero rate for a few seconds

Contents Retrieved from Microscopy Listserver Archives
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

Sylvain,

I had a similar problem in a WinDISS EDS system some years ago. The problem was a dead DC/DC
converter in the pre-amp at the detector. Easy, cheap fix once diagnosed.
I suspect your issue is something like this.

Phil
-----------------------------------------
Philip Oshel
Imaging Center Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576 office​
(989) 774-7567 lab



________________________________________
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Sent: Friday, July 24, 2020 17:21
To: Oshel, Philip Eugene

X-from: Sylvain Poudrette {spoudrette-at-videotron.ca}

Hello

Problem: EDX counts fluctuating down to zero rate for a few seconds
Anyone experienced this before ?

The beam current measured at stage with A Keithley meter and illumination from SE are very stable.
So I suspect this LN2 EDX is going to need repair. I just had it pumped down to a very good vacuum
level hence rid of any ice.

Sylvain

Private owner / hobbyist with an Amray 1850
& Isis EDX

Sent from my iPhone

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From: diller-at-stefan-diller.com
Date: Sun, 26 Jul 2020 11:16:41 -0500
Subject: [Microscopy] Re: viaWWW: SEM Filament Vibration

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Hello Mike,
EMI effects can be easily checked when you go to a low Working Distance like 6 mm and set SYNC on scan at 50 or 60 Hz (whatver your power grid has). If effect decreases you have some EMI influence.
Using a magnetic fiekd cancellation system like a Spicer 22 will greatly help.
Only uf this is ruled out then it might be something as strange as „filament vibration“...
Is it dependent on HV value?
It might also be a problem with the scan amp or a power supply there.

Best wishes
Stefan

-------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D-97072 Wuerzburg Germany
Tel. +49-931-7848700
Fax +49-931-7848701
Mobile +49-175-7177051

Email: diller-at-stefan-diller.com
Websites: www.stefan-diller.com
www.elektronenmikroskopie.info
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--------------------------------------------

} Am 26.07.2020 um 17:42 schrieb microscopy.listserver-at-gmail.com:
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} Email: miketoalson-at-gmail.com Name: Mike Toalson
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} Title-Subject: [Filtered] SEM Filament Vibration
}
} Message: Looking for feedback from the EM community. It was recently suggested to me that what
} appears to be wave like image distortion normally attributed to EMI could actually be induced by
} vibration of the Tungsten filament that results from incorrect centering or height adjustment in
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From: microscopy.listserver-at-gmail.com
Date: Mon, 27 Jul 2020 07:05:32 -0500
Subject: [Microscopy] Fwd: Link EDX counts fluctuating down to zero rate for a few seconds

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X-from: V.Ray {vray-at-partbeamsystech.com}

Hi Mike,

Noise is the usual subject for bickering between users complaining about noise and OEMs unwilling
(or being unable) to either resolve the issue with instrument, or convincingly demonstrate that
problem originates from the environment. Fortunately there are a few tests you can run on the SEM to
point in the direction of the root-cause:

1. As already suggested, change working distance. If the root cause is EMI picked up by the electron
beam, then larger WD will have increased noise. Same will happen if the root cause is mechanical
vibration of the source. But if source of the noise is in mechanical vibrations picked up by the
stage or body of the instrument then magnitude of vibrations wouldn't depend on working distance.

2. Change acceleration voltage by a lot, like 3kV to 30kV (if you can). If the source of noise is
EMI picked up by the beam, then lower-energy electrons would be more susceptible to it, and
magnitude of the noise with lower acceleration voltage would increase.

3. Go through the range of acceleration voltages. If the source of noise some kind of resonance or
cyclic process taking place with the source, or oscillations in high-voltage power supply, then you
may see not only magnitude, but also frequency of the noise change.

4. Tap gently on SEM column with the screwdriver. You will see additional noise, but look instead
how such tapping affects the original noise that was present. If tapping enhances original noise, or
maybe suppresses it for some time, then the likely cause is some kind of mechanical resonance in the
column or with the source.

5. Get a stethoscope (or surface-pickup microphone) and listen to sounds of the column. If you hear
something appearing when the source is up, and disappearing when the source is off then there is a
possibility of root cause being associated with the source operation (oscillations, resonance, etc...).

6. Connect that surface-pickup microphone to audio input of your laptop and run audio spectrum
analyzer - frequency and it changes may point to the root cause. For example, if you see frequency
matching rotation of your turbo then that could be the original source of noise.

7. Connect spectrum analyzer to the imaging output of your SEM. You will see frequencies associated
with line scanning, but look for something related to 50/60Hz or 100/120Hz - would be indicative of
poor filtration or a ground loop somewhere. Do this with electron beam blanked, open, and scanning -
differences could pin-point which part of the SEM circuitry is affected.

8. I spectrum analyzer is way too exotic animal then try working out frequency of the noise from
periodicity of waves and how it changes with change of the scanning frequency.

9. Switching frequency of high-voltage power supply or intermodulation between switching frequencies
of its various modules could be the culprit, if decoupling or filtration is inadequate.

10. Change imaging modes of the SEM. If noise is present in one of the modes and not in another -
root cause is in SEM electronics.

11. Everything else fails - get engineering help from someone with experience in noise troubleshooting.

Cheers :)
Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
www.partbeamsystech.com
www.fibsemproducts.com
www.freudlabs.com

On 7/26/2020 11:21 AM, microscopy.listserver-at-gmail.com wrote:
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} Title-Subject: [Filtered] SEM Filament Vibration
}
} Message: Looking for feedback from the EM community. It was recently suggested to me that what
} appears to be wave like image distortion normally attributed to EMI could actually be induced by
} vibration of the Tungsten filament that results from incorrect centering or height adjustment in
} relation to the Wehnelt.
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From normanatlas54-at-gmail.com Sun Jul 26 16:35:55 2020
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X-from: Pat Kelly {probe-at-geotrack.com.au}

We had this problem with our very old but still working EDAX detector recently. Spectra collecting
as normal and then suddenly no counts for a few seconds and then back to counting. We tracked it
down to the HV supply to the detector. (-750V for our detector)
It may be worth checking, make sure you use a high voltage probe with a high input impedence to measure.

Good Luck.

Patrick R Kelly Operations Manager
Geotrack International Pty Ltd ABN16 006 821 209
37 Melville Road, Brunswick West, Victoria 3055 Australia
Telephone: +613 93801077 Facsimile: +613 93801477
http://www.geotrack.com.au

-----Original Message----- From: microscopy.listserver-at-gmail.com
Sent: Saturday, July 25, 2020 7:21 AM
To: probe-at-geotrack.com.au

X-from: Sylvain Poudrette {spoudrette-at-videotron.ca}

Hello

Problem: EDX counts fluctuating down to zero rate for a few seconds
Anyone experienced this before ?

The beam current measured at stage with A Keithley meter and illumination from SE are very stable.
So I suspect this LN2 EDX is going to need repair. I just had it pumped down to a very good vacuum
level hence rid of any ice.

Sylvain

Private owner / hobbyist with an Amray 1850
& Isis EDX

Sent from my iPhone

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From: microscopy.listserver-at-gmail.com
Date: Mon, 27 Jul 2020 20:16:17 -0500
Subject: [Microscopy] viaWWW:RMC MT6000XL Manual

Contents Retrieved from Microscopy Listserver Archives
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Name: Ali Husain

Organization: University of Illinois at Urbana-Champaign
Title-Subject: [Filtered] RMC MT6000XL Manual?

Message: Hi all,

I managed to pull an old RMC MT6000XL ultramicrotome from a scrapper and
plan to work on it to make is usable. *Does anyone have a copy of the
manual for this microtome (or a related model)?* It's so old that I'm even
having trouble finding the specifications for it.

Thanks
Dr. Ali Husain
University of Illinois


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From: microscopy.listserver-at-gmail.com
Date: Thu, 30 Jul 2020 19:34:31 -0500
Subject: [Microscopy] viaWWW: ETEC sem filaments

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




-------- Forwarded Message --------
X-from: Mazurkiewicz, Joseph {MazurkJ-at-amc.edu}


I do. Im on vacation until Aug 10th. Ill scan and send a pdf whe I get back home.

Joe Mazurkiewicz
mazurkj-at-amc.edu



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Email: ali.ibn.rafi-at-gmail.com

Name: Ali Husain

Organization: University of Illinois at Urbana-Champaign
Title-Subject: [Filtered] RMC MT6000XL Manual?

Message: Hi all,

I managed to pull an old RMC MT6000XL ultramicrotome from a scrapper and
plan to work on it to make is usable. *Does anyone have a copy of the
manual for this microtome (or a related model)?* It's so old that I'm even
having trouble finding the specifications for it.

Thanks
Dr. Ali Husain
University of Illinois


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From janiceking866isobi-at-gmail.com Thu Jul 30 05:47:37 2020
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Email: dale_cornwell-at-yahoo.com

Name: Dale Cornwell

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Title-Subject: [Filtered] etec sem filaments

Message: I have a box of ten filaments. anyone interested?
also a 545 film holder

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From: microscopy.listserver-at-gmail.com
Date: Sun, 2 Aug 2020 17:49:01 -0500
Subject: [Microscopy] viaWWW: Postdoc/Research Associate position(s) available

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Jeffrey Streger {jeff-at-ravescientific.com}

Wow,

545 film holder. We are showing our age on this one. Remember when one out of the 20 pieces of film
would get stuck and then of course one had to clean the developing chemicals out of the holder?
With best regards,

Jeff


} On Jul 30, 2020, at 8:50 PM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:
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} Name: Dale Cornwell
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} Title-Subject: [Filtered] etec sem filaments
}
} Message: I have a box of ten filaments. anyone interested?
} also a 545 film holder
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From ketcdutj170faazi-at-gmail.com Sun Aug 2 12:29:25 2020
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X-from: bassimn-at-mcmaster.ca

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Email: bassimn-at-mcmaster.ca Name: Nabil Bassim

Organization: McMaster University

Title-Subject: [Filtered] Postdoc/Research Associate position(s) available

Message: The Bassim research group is looking for up to 4 postdocs to start in the next 6 months.
All work would be performed at the Canadian Centre for Electron Microscopy (CCEM) - (web -
ccem.mcmaster.ca).

The first position is in TEM of 2-D materials, including sample preparation, EELS mapping of
structures, and high resolution STEM imaging. We are seeking a Postdoc or Research Associate who
will work on a varied portfolio of projects, including developing novel 2-D van der Waals
heterostructures.

The second position is related to in-situ TEM electrochemistry experiments to monitor the
microstructural evolution of catalysts.

The 3rd position is a hybrid of optical materials, materials synthesis and low-loss EELS
characterization of plasmonic and phononic structures.

The 4th position is in developing Focused Ion Beam techniques, including patterning, serial
sectioning and an exploration of ion-sample interactions.

The chosen candidates would also be encouraged to develop their own microscopy techniques using the
CCEM infrastructure. The research projects involve strong collaboration with synthesis teams, and
proven ability to work in interdisciplinary groups should be demonstrated. Teaching, communication,
and presentation skills are part of a successful post-doctoral or research associate position.
Day-to-day supervisory tasks with graduate students in the CCEM and within the Bassim research group
would be expected.

Requirements for the role include:

MANDATORY

- A Ph.D. in Materials Science or Physics

- A strong track record in publishing TEM-related research

DESIRED

- Interest in mentoring graduate students

- - Experience in aberration-corrected imaging and electron energy loss spectroscopy

- Experience with in-situ techniques

- Capability to perform image simulations and scattering theory

- Sample preparation capabilities using FIB



Pay will range based on level of experience and track record. The appointments are for 1 year,
renewable up to 3 years.

Located at McMaster University in Hamilton, Ontario, the CCEM is home to 10 technical staff and
hundreds of users with many diverse interests. Hamilton is a lovely city in Southern Ontario,
located midway between Toronto and Niagara Falls with a mild (by Canadian standards) winter.

If you are interested, please contact bassimn at mcmaster.ca with a CV and several references.Erase
this text and type your question here

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From: nizets2-at-yahoo.com
Date: Tue, 4 Aug 2020 03:55:02 -0500
Subject: [Microscopy] Epon Embedding big samples

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello dear colleagues over the world!


I am going to have to embed skin biopsies in Epon. To give you an idea of the size and form of the sample, imagine a cylinder 6mm in diameter and 6mm deep, then cut this cylinder in 4 pieces along the length. You'll 1/4 of a cylinder with 3mm width and 6mm length. This is quite big to embed but I cannot cut it further, believe me.


I always prepared my samples in Epon with one dimension being at most 1mm, this is the first time that I have to embed such big samples.
Fortunately the skin is not that hard so penetration shouldn't be so difficult after all, I just need to adapt the incubation times to be sure that the samples will be entirely perfectly embedded.


Did somebody already deal with samples that size? Which incubation durations would you advise ?
The samples have been already kept in fixative (PFA+Glut) for several weeks at 4°C. 
I want to post-fix in OsO4, dehydrate in acetone and go directly from acetone in Epon.
I plan to cure the resin for 3 days at 60-70°C.
Thank you in advance!


Stay safe!
Stephane


==============================Original Headers==============================
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10, 35 -- Date: Tue, 4 Aug 2020 09:02:28 +0000 (UTC)
10, 35 -- From: Stephane Nizet {nizets2-at-yahoo.com}
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From: wij.muss-at-aon.at
Date: Tue, 4 Aug 2020 04:50:58 -0500
Subject: [Microscopy] Re: Epon Embedding big samples

Contents Retrieved from Microscopy Listserver Archives
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Dear Stephane,
You might find (perhaps!) helpful my general approaches to skin biopsies of
that size...(to be cut 'semithin' with a Histo-Diamond Knive edge length 6
mm, from a well known company....Mr. H.Gnaegi!) for correlative TEM Diamond
knive -same company - cutting edge 4-5 mm...it is possible to take ultrathin
sections e.g. 1.5-2 mm width x 4-5 mm length)
in:
https://www.researchgate.net/profile/Wolfgang_MUSS/publications:

Restoration of functional skin at an inveterate large ulceration site in a
patient with junctional epidermolysis bullosa (EB-J) using autologous,
genetically corrected keratinocytes: immunohistochemical, light and electron
microscopy findings
(https://www.researchgate.net/publication/326045619_Restoration_of_functiona
l_skin_at_an_inveterate_large_ulceration_site_in_a_patient_with_junctional_e
pidermolysis_bullosa_EB-J_using_autologous_genetically_corrected_keratinocyt
es_immunohistochemical_l)

and related Poster
https://www.researchgate.net/publication/326045434_Restoration_of_functional
_skin_at_an_inveterate_large_ulceration_site_in_a_patient_with_junctional_ep
idermolysis_bullosa_EB-J_using_autologous_genetically_corrected_keratinocyte
s_immunohistochemical_l

If you want more detailed info on how I processed tissue specs and performed
those embeddings and sectioning (semithin & ultrathin) just request.
Greetings,
best regards


STAY SAFE- Keep spatial not social Distance STAY HEALTY
Wolfgang

=============================================
MUSS Wolfgang Dr. phil./PhD [OR i. R./retired]

Ignaz-Rieder-Kai 19/6
A-5020 SALZBURG
sterreich-AUSTRIA
Mobile-Tel.: 0043(0)676 5 369 456
E-mail: wij.muss-at-aon.at
E-Mail altern.: womuss-at-gmail.com

FRMS, Retired Member of MSA & other (Inter-)National Societies

Former Head of Electron Microscopy Lab at Institute of Pathology
SALK-LKH / Salzburger Landeskliniken | General Hospital and
PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG

'Scientific' Profile including 'publications' at ResearchGate:
http://www.researchgate.net/profile/Wolfgang_MUSS
inviting you to join ResGate.
(Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html,
and join 14+ million researchers, including 63 Nobel Laureates


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Hello dear colleagues over the world!


I am going to have to embed skin biopsies in Epon. To give you an idea of
the size and form of the sample, imagine a cylinder 6mm in diameter and 6mm
deep, then cut this cylinder in 4 pieces along the length. You'll 1/4 of a
cylinder with 3mm width and 6mm length. This is quite big to embed but I
cannot cut it further, believe me.


I always prepared my samples in Epon with one dimension being at most 1mm,
this is the first time that I have to embed such big samples.
Fortunately the skin is not that hard so penetration shouldn't be so
difficult after all, I just need to adapt the incubation times to be sure
that the samples will be entirely perfectly embedded.


Did somebody already deal with samples that size? Which incubation durations
would you advise ?
The samples have been already kept in fixative (PFA+Glut) for several weeks
at 4°C. I want to post-fix in OsO4, dehydrate in acetone and go directly
from acetone in Epon.
I plan to cure the resin for 3 days at 60-70°C.
Thank you in advance!


Stay safe!
Stephane


==============================Original Headers==============================
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26, 20 -- Subject: [Microscopy] Re: Epon Embedding big samples
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Email: ahk-at-bu.edu Name: Anlee Krupp

Organization: Boston University

Title-Subject: [Filtered] EDAX Genesis manual

Message: Hi all,

We replaced our EDAX EDS unit using Genesis software couple years ago, and about to recycle the
manual at end of August.
Please let me know if you're interested in getting them. They are:
1. EDAX Genesis User's manual
2. Genesis Service/Installation manual
3. TSL (EBSD) operators training course

Thanks.

Anlee Krupp
Laboratory Manager
Precision Measurement Laboratory
Boston University Photonics Center
8 Saint Marys Street, 9th Floor
email: ahk-at-bu.edu


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From: microscopy.listserver-at-gmail.com
Date: Tue, 4 Aug 2020 07:34:24 -0500
Subject: [Microscopy] viaWWW: Postdoc position available on in-situ environmental (S)TEM

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Email: yuanyuan.2.zhu-at-uconn.edu Name: Yuanyuan Zhu

Organization: University of Connecticut

Title-Subject: [Filtered] Postdoc position available on in-situ environmental (S)TEM

Message: The University of Connecticut (UConn), one of the top 20 public universities in the nation,
invites applications for a Postdoctoral Research Associate position in the Department of Materials
Science and Engineering (MSE) in the School of Engineering and the Institute of Materials Science
(IMS). Applicants with a strong background in (scanning) transmission electron microscopy ((S)TEM)
and in-situ experiment experiences are encouraged to apply. In this position, you will have the
opportunity to engage creative research on investigating structure-property dynamics in advanced
functional and structural materials including but not limited to heterogeneous catalysts. You will
have the opportunity to work in a state-of-the-art TEM center hosting a probe-corrected Titan Themis
STEM, and perform in-situ (gaseous) environmental microscopy at our latest InToEM center
(https://today.uconn.edu/school-stories/intoem/).
DUTIES AND RESPONSIBILITIES
The successful candidate will share a deep commitment to transmission electron microscopy. Working
under the supervision of Prof. Yuanyuan Zhu, the candidate will be expected to lead in-situ TEM
researches to further the understanding of materials dynamics. The candidate will also contribute to
TEM sample preparation, mentor graduate and undergraduate students; write progress reports; interact
with research collaborators; prepare and maintain lab equipment and supplies, submit and publish
peer reviewed journal papers.
MINIMUM QUALIFICATIONS
An earned doctorate in Materials Science, Chemistry, Physics or a related discipline. A strong
background and extensive research experience in TEM sample preparation and (scanning) transmission
electron microscopy characterization. Good written and verbal communication skills. Good research
capabilities as evidenced by a record of publication of results in peer-reviewed journals and
external presentations at scientific conferences.

PREFERRED QUALIFICATIONS
Additionally, a strong background in one or several of these fields is desirable: heterogeneous
catalysis, surface science, solid state physics. The candidate is expected to be proficient at three
or more of the following techniques including but not limited to: SAED, Nanobeam Electron
Diffraction, HRTEM, probe-corrected STEM, EDS mapping, core- (and low-) loss EELS, in-situ heating
TEM. Skills and experience in in-situ gas-cell microscopy is highly desired. Strong interpersonal
skills including the ability to interact effectively with staffs, students and collaborators.
APPOINTMENT TERMS
The selected candidate is expected to start immediately or upon mutual agreement. This is a
full-time (12-month appointment) position, and is renewable every year. The successful candidates
primary academic appointment will be at the UConn main campus in Storrs, CT. Salary will
commensurate with qualifications and experience. 

TO APPLY
Please submit the following: a cover letter; curriculum vitae (with a full list of publication),
copies of two representative publications to yuanyuan.2.zhu-at-uconn.edu, with a subject title
In-situTEMPostdoc_yourname.
Evaluation of applicants will begin immediately and continue until the position is filled.
Employment of the successful candidate will be contingent upon the successful completion of a
pre-employment criminal background check.

All employees are subject to adherence to the State Code of Ethics, which may be found at
http://www.ct.gov/ethics/site/default.asp.


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From: microscopy.listserver-at-gmail.com
Date: Thu, 6 Aug 2020 08:01:44 -0500
Subject: [Microscopy] viaWWW:Surface Analysis Scientist - The University of Queensland

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X-from: g.auchterlonie-at-uq.edu.au


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Email: g.auchterlonie-at-uq.edu.au Name: Graeme Auchterlonie

Organization: Centre for Microscopy & Microanalysis, UQ

Title-Subject: [Filtered] Surface Analysis Scientist - The University of Queensland

Message: Surface Analysis Scientist: Applications close: 18 Aug 2020 (11:00 PM) E. Australia
Standard Time

About this opportunity:
CMM provides a broad range X-ray diffraction, spectroscopy and imaging services to UQ, including
state-of-the-art X-ray Photoelectron Spectroscopy (XPS) with insitu inoperando and correlative
capabilities. UQ requires a highly-skilled and experienced Surface Analysis Scientist to both
operate and maintain the instrument and to train clients in the design of experiments for, the
operation of, and the analysis and interpretation of data from the XPS instrument. In addition, the
role will also provide support for the provision of other advance X-ray analysis techniques
including XRD, XRF and/or SAXS.
The Surface Analysis Scientist needs to be capable of operating effectively within a team
environment but also with a high degree of independence. They will need to provide a very high level
of expertise in the areas of surface science analysis, and advance X-ray characterisation to support
the research groups within UQ, which have a strong focus on the understanding and engineering of
material-, chemical- or bio-interfaces.
The role will also service the future needs of the next generation of researchers through research
into and the development of new and/or enhanced experimental methods, instrumental capabilities and
workflows (including data analysis) relevant to these disciplines.

Our ideal candidate:
The successful candidate will be a creative, surface scientist and dedicated XPS specialist with a
strong interest in the development of new methods in their field of expertise.
You will be driven by the desire to understand the molecular structure, chemistry and dynamics of
surfaces and how to accurately elucidate these through the latest advances in scientific methods.
You will be client focused, enjoy sharing their knowledge and ready to contribute to our team.
Although current work rights in Australia would be an advantage, CMM welcomes applications from
candidates who do not yet have a work permit. However, any appointment will be conditional to the
candidate having secured unconditional work rights in Australia.
We value diversity and inclusion, and actively encourage applications from those who bring
diversity to the University. Our Diversity and Inclusion webpage contains further information if you
require additional support. Accessibility requirements and/or adjustments can be directed to
recruitment-at-uq.edu.au.

Please see http://search.jobs.uq.edu.au/caw/en/job/509618/surface-analysis-scientist for full
details and to apply.

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From: microscopy.listserver-at-gmail.com
Date: Thu, 6 Aug 2020 08:02:32 -0500
Subject: [Microscopy] viaWWW:Glow discharge for TEM grids

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Email: stefano-at-soquelec.com Name: Stefano Rubino

Title-Subject: [Filtered] Glow discharge for TEM grids

Message: Hi,
We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like
to use it for making TEM grids hydrophilic.
We would appreciate any suggestion or comment on how to determine the best parameters for this process.

Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I
am wondering if this can cause any differences.

What is an optimal height of the stage or the distance from the bottom surface of the glow discharge
insert to the surface of the stage/TEM grids?

In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any
comments on how those parameters affect the process?
Thank you in advance and best regards,
Stefano Rubino

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From: microscopy.listserver-at-gmail.com
Date: Thu, 6 Aug 2020 08:29:09 -0500
Subject: [Microscopy] viaWWW:Glow discharge for TEM grids

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Email: stefano-at-soquelec.com Name: Stefano Rubino

Title-Subject: [Filtered] Glow discharge for TEM grids

Message: Hi,
We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like
to use it for making TEM grids hydrophilic.
We would appreciate any suggestion or comment on how to determine the best parameters for this process.

Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I
am wondering if this can cause any differences.

What is an optimal height of the stage or the distance from the bottom surface of the glow discharge
insert to the surface of the stage/TEM grids?

In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any
comments on how those parameters affect the process?
Thank you in advance and best regards,
Stefano Rubino

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Listserver Email Form V - 20120416
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From: microscopy.listserver-at-gmail.com
Date: Thu, 6 Aug 2020 08:42:14 -0500
Subject: [Microscopy] viaWWW:Surface Analysis Scientist - The University of Queensland

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Email: g.auchterlonie-at-uq.edu.au Name: Graeme Auchterlonie

Organization: Centre for Microscopy & Microanalysis, UQ

Title-Subject: [Filtered] Surface Analysis Scientist - The University of Queensland

Message: Surface Analysis Scientist: Applications close: 18 Aug 2020 (11:00 PM) E. Australia
Standard Time

About this opportunity:
CMM provides a broad range X-ray diffraction, spectroscopy and imaging services to UQ, including
state-of-the-art X-ray Photoelectron Spectroscopy (XPS) with insitu inoperando and correlative
capabilities. UQ requires a highly-skilled and experienced Surface Analysis Scientist to both
operate and maintain the instrument and to train clients in the design of experiments for, the
operation of, and the analysis and interpretation of data from the XPS instrument. In addition, the
role will also provide support for the provision of other advance X-ray analysis techniques
including XRD, XRF and/or SAXS.
The Surface Analysis Scientist needs to be capable of operating effectively within a team
environment but also with a high degree of independence. They will need to provide a very high level
of expertise in the areas of surface science analysis, and advance X-ray characterisation to support
the research groups within UQ, which have a strong focus on the understanding and engineering of
material-, chemical- or bio-interfaces.
The role will also service the future needs of the next generation of researchers through research
into and the development of new and/or enhanced experimental methods, instrumental capabilities and
workflows (including data analysis) relevant to these disciplines.

Our ideal candidate:
The successful candidate will be a creative, surface scientist and dedicated XPS specialist with a
strong interest in the development of new methods in their field of expertise.
You will be driven by the desire to understand the molecular structure, chemistry and dynamics of
surfaces and how to accurately elucidate these through the latest advances in scientific methods.
You will be client focused, enjoy sharing their knowledge and ready to contribute to our team.
Although current work rights in Australia would be an advantage, CMM welcomes applications from
candidates who do not yet have a work permit. However, any appointment will be conditional to the
candidate having secured unconditional work rights in Australia.
We value diversity and inclusion, and actively encourage applications from those who bring
diversity to the University. Our Diversity and Inclusion webpage contains further information if you
require additional support. Accessibility requirements and/or adjustments can be directed to
recruitment-at-uq.edu.au.

Please see http://search.jobs.uq.edu.au/caw/en/job/509618/surface-analysis-scientist for full
details and to apply.

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From: benada-at-biomed.cas.cz
Date: Thu, 6 Aug 2020 10:09:56 -0500
Subject: [Microscopy] Re: viaWWW:Glow discharge for TEM grids

Contents Retrieved from Microscopy Listserver Archives
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Hello Stefano,
We are using an old Polaron sputter-coater for glow-discharge
activation of carbon or carbon/formvar coated grids. We have modified
it for glow-discharge over 30 years ago and since then we are using it
at following settings:

Electrode distance = 40 mm
Current = 10 mA (DC)
Time = 30 seconds
Pressure = 0.1 torr (reduced air atmosphere)

Grids are placed exactly at the border of Crookes dark space on a
support made of a mica sheet.

I hope this could help you in setting up your Q150T ES.


Regards Oldrich

P.S. We published a paper on modification of our Polaron sputter-coater
in "Microscopy Research and Technique" 30 years ago. If you would like,
I could send you a link.


--
Oldřich Benada
Institute of Microbiology, Czech Acad. Sci.
Laboratory of Molecular Structure Characterization
Electron Microscopy Group
Vídeňská 1083
142 20 Prague 4
Czech Republic

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} Title-Subject: [Filtered] Glow discharge for TEM grids
}
} Message: Hi,
} We have a Quorum coater (Q150T ES - turbopumped) with the glow
} discharge attachment. We would like to use it for making TEM grids
} hydrophilic. We would appreciate any suggestion or comment on how to
} determine the best parameters for this process.
}
} Should we use air or Argon as the process gas? Currently, both gas
} inlets are hooked to Argon, and I am wondering if this can cause any
} differences.
}
} What is an optimal height of the stage or the distance from the
} bottom surface of the glow discharge insert to the surface of the
} stage/TEM grids?
}
} In the default recipe, the time is set at 25 seconds and the current
} at 25 mA, does anyone have any comments on how those parameters
} affect the process? Thank you in advance and best regards,
} Stefano Rubino
}
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==============================Original Headers==============================
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From: wpchan-at-uw.edu
Date: Thu, 6 Aug 2020 15:48:48 -0500
Subject: [Microscopy] Fwd: viaWWW:Glow discharge for TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stefano

Quorum support provided me with the following parameters for glow
discharge using the glow discharge insert (10262) on a Q150T ES. I
mainly use this function to prepare grids for negative staining.

distance: 35 mm below the insert (optimal 30-35 mm, shorter distance
takes shorter duration)
current: 20 mA, 25-30 mA max
duration: 20 s
gas: room air (I use a filter on the air inlet)

If you overcook it e.g., excess time or current, too close to the
insert, you can burn the carbon and result in cracks. In addition, if
you use Ar, you will just etch the coating. Cheers!

--
Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519)
The Biology Imaging Facility
(https://www.biology.washington.edu/facilities/research/imaging)


On Thu, Aug 6, 2020 at 6:41 AM {microscopy.listserver-at-gmail.com} wrote:
}
} Email: stefano-at-soquelec.com Name: Stefano Rubino
}
} Title-Subject: [Filtered] Glow discharge for TEM grids
}
} Message: Hi,
} We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like
} to use it for making TEM grids hydrophilic.
} We would appreciate any suggestion or comment on how to determine the best parameters for this process.
}
} Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I
} am wondering if this can cause any differences.
}
} What is an optimal height of the stage or the distance from the bottom surface of the glow discharge
} insert to the surface of the stage/TEM grids?
}
} In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any
} comments on how those parameters affect the process?
} Thank you in advance and best regards,
} Stefano Rubino

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Thu, 6 Aug 2020 21:58:57 -0500
Subject: [Microscopy] Fwd: Fwd: viaWWW:Glow discharge for TEM grids

Contents Retrieved from Microscopy Listserver Archives
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X-from: wpchan-at-uw.edu



----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Hi Stefano

Quorum support provided me with the following parameters for glow
discharge using the glow discharge insert (10262) on a Q150T ES. I
mainly use this function to prepare grids for negative staining.

distance: 35 mm below the insert (optimal 30-35 mm, shorter distance
takes shorter duration)
current: 20 mA, 25-30 mA max
duration: 20 s
gas: room air (I use a filter on the air inlet)

If you overcook it e.g., excess time or current, too close to the
insert, you can burn the carbon and result in cracks. In addition, if
you use Ar, you will just etch the coating. Cheers!

--
Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519)
The Biology Imaging Facility
(https://www.biology.washington.edu/facilities/research/imaging)


On Thu, Aug 6, 2020 at 6:41 AM {microscopy.listserver-at-gmail.com} wrote:
}
} Email: stefano-at-soquelec.com Name: Stefano Rubino
}
} Title-Subject: [Filtered] Glow discharge for TEM grids
}
} Message: Hi,
} We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like
} to use it for making TEM grids hydrophilic.
} We would appreciate any suggestion or comment on how to determine the best parameters for this process.
}
} Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I
} am wondering if this can cause any differences.
}
} What is an optimal height of the stage or the distance from the bottom surface of the glow discharge
} insert to the surface of the stage/TEM grids?
}
} In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any
} comments on how those parameters affect the process?
} Thank you in advance and best regards,
} Stefano Rubino
==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Thu, 6 Aug 2020 22:00:25 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:Glow discharge for TEM grids

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Oldrich Benada {benada-at-biomed.cas.cz}

Hello Stefano,
We are using an old Polaron sputter-coater for glow-discharge
activation of carbon or carbon/formvar coated grids. We have modified
it for glow-discharge over 30 years ago and since then we are using it
at following settings:

Electrode distance = 40 mm
Current = 10 mA (DC)
Time = 30 seconds
Pressure = 0.1 torr (reduced air atmosphere)

Grids are placed exactly at the border of Crookes dark space on a
support made of a mica sheet.

I hope this could help you in setting up your Q150T ES.


Regards Oldrich

P.S. We published a paper on modification of our Polaron sputter-coater
in "Microscopy Research and Technique" 30 years ago. If you would like,
I could send you a link.


--
Oldřich Benada
Institute of Microbiology, Czech Acad. Sci.
Laboratory of Molecular Structure Characterization
Electron Microscopy Group
Vídeňská 1083
142 20 Prague 4
Czech Republic

On Thu, 6 Aug 2020 08:30:26 -0500, microscopy.listserver-at-gmail.com
wrote :
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} Email: stefano-at-soquelec.com Name: Stefano Rubino
}
} Title-Subject: [Filtered] Glow discharge for TEM grids
}
} Message: Hi,
} We have a Quorum coater (Q150T ES - turbopumped) with the glow
} discharge attachment. We would like to use it for making TEM grids
} hydrophilic. We would appreciate any suggestion or comment on how to
} determine the best parameters for this process.
}
} Should we use air or Argon as the process gas? Currently, both gas
} inlets are hooked to Argon, and I am wondering if this can cause any
} differences.
}
} What is an optimal height of the stage or the distance from the
} bottom surface of the glow discharge insert to the surface of the
} stage/TEM grids?
}
} In the default recipe, the time is set at 25 seconds and the current
} at 25 mA, does anyone have any comments on how those parameters
} affect the process? Thank you in advance and best regards,
} Stefano Rubino
}
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From: microscopy.listserver-at-gmail.com
Date: Thu, 6 Aug 2020 22:00:57 -0500
Subject: [Microscopy] viaWWW:Glow discharge for TEM grids

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X-from: s.walck-at-comcast.net

Stefano,
I don't process grids, but I can tell you what is going on with your glow
discharge. To make the surface hydrophilic, you are cleaning the
hydrocarbons from the surface. You need oxygen to do that well, which you
can get from air. Argon can give you some cleaning, but it is not the best
process gas. If you were to use pure oxygen, your pumps have to be rated
for oxygen. Air or a mixture of Ar-25%O2 is safe for any mechanical pump
that uses a hydrocarbon based oil. What is cleaning the grids is activated
oxygen molecules which is causing the hydrocarbons to break down and H2O and
CO2 to form, which is pumped away. Plasma cleaners do the same thing. The
difference between plasma cleaning and glow discharge is the energy of the
species that strike the surface. In an RF plasma cleaner, the energy is
relatively low. In a DC glow discharge, the energy is higher and it can
warm the sample. With copper grids, who cares.

With respect to your distance, I don't know that system. However, as long
as the grids are in the plasma, they won't take long to clean. Plasma
cleaning is usually 5 minutes or less. I would suspect that glow discharge
takes about the same amount of time or less. If you want to experiment with
your system, take a piece of material such as copper tape, silicon wafer, or
perhaps even a glass slide (I don't know whether that will charge in a DC
plasma.) . Put a drop of distilled water on the surface and you will see
that it beads up on the surface. Because of the hydrocarbons on the
surface, the surface is hydrophobic. It has a high contact angle. After
processing, a water droplet will wet out the surface and spread. That is
hydrophilic and the contact angle is low. To test how long it takes for a
given distance in your coater, use that test surface and see how long it
takes to process to give a hydrophilic surface. So for your process
parameters, just try them on that test surface. If it doesn't work, do it
multiple times until it does and then go from there.

-Scott Walck

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Email: stefano-at-soquelec.com Name: Stefano Rubino

Title-Subject: [Filtered] Glow discharge for TEM grids

Message: Hi,
We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge
attachment. We would like to use it for making TEM grids hydrophilic.
We would appreciate any suggestion or comment on how to determine the best
parameters for this process.

Should we use air or Argon as the process gas? Currently, both gas inlets
are hooked to Argon, and I am wondering if this can cause any differences.

What is an optimal height of the stage or the distance from the bottom
surface of the glow discharge insert to the surface of the stage/TEM grids?

In the default recipe, the time is set at 25 seconds and the current at 25
mA, does anyone have any comments on how those parameters affect the
process?
Thank you in advance and best regards,
Stefano Rubino

Login Host: 207.216.59.87
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From: microscopy.listserver-at-gmail.com
Date: Fri, 14 Aug 2020 07:29:25 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Sectioning beetle elytra

Contents Retrieved from Microscopy Listserver Archives
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X-from: Michel Ribardire {m.ribardiere-at-jeol.fr}



In old time we use an air leak to make plasma and only Rotary Pump to make vacuum to make
hydrophilic spcimens.
The spcimen was set in place of the mtal deposit target.
Michel




Envoy de mon Galaxy A5 2017 Orange



-------- Message d'origine --------
De : microscopy.listserver-at-gmail.com
Date : 06/08/2020 15:41 (GMT+01:00)
: Michel Ribardire {m.ribardiere-at-jeol.fr}
Objet : [Microscopy] viaWWW:Glow discharge for TEM grids




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Email: stefano-at-soquelec.com Name: Stefano Rubino

Title-Subject: [Filtered] Glow discharge for TEM grids

Message: Hi,
We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like
to use it for making TEM grids hydrophilic.
We would appreciate any suggestion or comment on how to determine the best parameters for this process.

Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I
am wondering if this can cause any differences.

What is an optimal height of the stage or the distance from the bottom surface of the glow discharge
insert to the surface of the stage/TEM grids?

In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any
comments on how those parameters affect the process?
Thank you in advance and best regards,
Stefano Rubino

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} On Aug 6, 2020, at 6:34 AM, microscopy.listserver-at-gmail.com wrote:
}
}
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} using the WWW based Form at http://microscopy.com/MLFormMail.html
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} Email: stefano-at-soquelec.com Name: Stefano Rubino
}
} Title-Subject: [Filtered] Glow discharge for TEM grids
}
} Message: Hi,
} We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like
} to use it for making TEM grids hydrophilic.
} We would appreciate any suggestion or comment on how to determine the best parameters for this process.
}
} Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I
} am wondering if this can cause any differences.
}
} What is an optimal height of the stage or the distance from the bottom surface of the glow discharge
} insert to the surface of the stage/TEM grids?
}
} In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any
} comments on how those parameters affect the process?
} Thank you in advance and best regards,
} Stefano Rubino

Hi Stefano,
Oxygen in the plasma interacts with the grid surface to make it hydrophilic, so make sure there is enough in the chamber during discharge. As to your other two questions, I would start with the default and check the grids; if a 3 ul droplet spreads evenly and quickly when applied to the grid, it is sufficiently hydrophilic, if not, increase the time until success.
Yours,
Bill





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Title-Subject: [Filtered] Sectioning beetle elytra

Message: I have some beetle wing covers (elytra) to embed and section for TEM. Anyone have success
with this? A typical embedment in EMBed812 (Epon) with a medium hardness is not holding the wing in
the section. I'm thinking either a harder formulation, or a different resin.
I don't believe fixing it like tissue would make a difference, however I'm not an expert in insect
cuticle preparation and sectioning.

Thanks!
John Shields
Georgia Electron Microscopy

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From ronasyre474bogyv-at-gmail.com Fri Aug 14 05:09:25 2020
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X-from: Paula Keene Pierce {paula-at-excaliburpathology.com}

Hi,

insect exoskeleton is mainly made of chitin.

To soften the chitin, after fixation, place the specimens in a liquid such as Lysol or Simple Green
Clean Finish that contains alkyl dimethylbenzylammonium chlorides for 20 min. to 1 hour.

No kidding! I use it for drosophila eyes in paraffin sections.

This works in the same way it kills bacteria, fungus, viruses...



Paula Keene Pierce, BS, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
5830 N Blue Lake Drive
Norman, OK 73069
PH 405-759-3953
http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb


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Title-Subject: [Filtered] Sectioning beetle elytra

Message: I have some beetle wing covers (elytra) to embed and section for TEM. Anyone have success
with this?  A typical embedment in EMBed812 (Epon) with a medium hardness is not holding the wing in
the section. I'm thinking either a harder formulation, or a different resin.
I don't believe fixing it like tissue would make a difference, however I'm not an expert in insect
cuticle preparation and sectioning.

Thanks!
John Shields
Georgia Electron Microscopy

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From: microscopy.listserver-at-gmail.com
Date: Fri, 14 Aug 2020 07:30:51 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Sectioning beetle elytra

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Calomeni, Edward {Edward.Calomeni-at-osumc.edu}

John,
This sounds like a Dear Abby question. I imagine an answer would likely entail the careful use of a
Ginsu Knife.
Sorry, cannot be of much further help with insect wings.

Congratulates on winning the Morton Maser award.

Ed
Edward P. Calomeni
Director EM Lab - Pathology
The Ohio State University
M018 Starling Loving Hall
320 W. 10th Ave.
Columbus, OH 43210
614-293-5580 (office)
614-293-8806 (lab)
edward.calomeni-at-osumc.edu

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, August 13,
2020 8:58 AM
To: Calomeni, Edward {Edward.Calomeni-at-osumc.edu}

X-from: Mowery, Joseph {joseph.mowery-at-usda.gov}

Hi John,

Adding 1% Z6040 to the ethanol during dehydration functions as a coupling agent to bind the resin
to the cuticle, and I can confirm it works on insect wings.

Explained in more detail in my recent M&M2020 article:
"Utilization of Z-6040 Organosilane as a Coupling Agent to Improve the Adhesion of Epoxy Resins to
Waxy Biological Tissues"
DOI: https://doi.org/10.1017/S143192762001781X

Best regards
- Joe

Joe Mowery | Biologist
Electron and Confocal Microscopy Unit
USDA Agricultural Research Service

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Sent: Thursday, August 13, 2020 8:59 AM
To: Mowery, Joseph {joseph.mowery-at-usda.gov}

X-from: Kelley,Karen L {vau-at-ufl.edu}

I would recommend adding Z6040 to the resin mixture. I’ve used Z6040 in epoxy, LRW and HM20
(100ul/10ml resin). It will greatly improve the sectioning.
Best of luck

Sent from my iPhone

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} Email: jpshield-at-uga.edu Name: John Shields
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} Organization: University of Georgia
}
} Title-Subject: [Filtered] Sectioning beetle elytra
}
} Message: I have some beetle wing covers (elytra) to embed and section for TEM. Anyone have success
} with this? A typical embedment in EMBed812 (Epon) with a medium hardness is not holding the wing in
} the section. I'm thinking either a harder formulation, or a different resin.
} I don't believe fixing it like tissue would make a difference, however I'm not an expert in insect
} cuticle preparation and sectioning.
}
} Thanks!
} John Shields
} Georgia Electron Microscopy
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From: microscopy.listserver-at-gmail.com
Date: Fri, 14 Aug 2020 08:27:56 -0500
Subject: [Microscopy] Administrivia: Looking for copies of the EMSA Proceedings from the

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Good Morning/Afternoon/Evening Colleagues around the world...

The Microscopy Society of America has over many years been working on a detailed project to
create a digital library containing copies of the proceedings of all our past meetings.

While our Archivist (Mike Marko) and several of us have a fairly extensive set of the
(E)MSA and MM proceedings we are missing a few issues to complete the library.

Does anyone have copies of the following proceedings which we can borrow scan and then return?

We are looking for:

EMSA Proceedings 1961 - Pittsburg
EMSA Proceedings 1965 - New York City
EMSA Proceedings 1966 - San Francisco

Also if anyone has brochures/pamphlets advertising the 1962 Philadelpha or 1964 Detroit EMSA
meetings we would also like to digitize and include them as part of our archives.

MSA will pay the cost for shipping, digitizing, and returning your proceedings.

To digitize the proceedings properly it will necessitate unbinding the hardcopy. The copies will,
of course, be rebound and returned.

If you have copies that you can loan us, then please contact Mike Marko {mike.marko.em-at-gmail.com}
and he will coordinate with you.


Thanks for your collective help on this.

Cheers

Nestor
Your Friendly Neighborhood SysOp






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24, 50 -- 1960's
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From: diller-at-stefan-diller.com
Date: Fri, 14 Aug 2020 08:58:33 -0500
Subject: [Microscopy] Oldies but Goldies - Looking for Lens Current Schematics on Zeiss EM9

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

to repair a drifting focus situation at a tube-driven Zeiss EM9 S2 TEM still doing it`s work a a German high-school I am searching
for the schematics on the generation of the lens currents.

I have part of it but I am missing a high-def PDF of:

- Lens Current Supply Functional circuit diagram 340600 Wart 64/65

- Lay-Out Ort 6 340600 Wart 31/32 (Component Layout Lens Current Supply - both sides)

- Lay-Out Ort 7 340600 Wart 29/30 (Component Layout Lens Shift - Astigmatism - Condensor - both sides)

It would be great if there is a chance to still get theses documents 55 years after the build.


All the best,

Stefan

--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------




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17, 29 -- Subject: Oldies but Goldies - Looking for Lens Current Schematics on Zeiss EM9
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From: stefan.diller-at-t-online.de
Date: Fri, 14 Aug 2020 14:11:16 -0500
Subject: [Microscopy] Oldies but Goldies - EM 9 schematics solved

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

I got the missing files in the meantime.


Thanks,

Stefan

--


-----------------------------------------------------
Stefan Diller - Scientific Photography
Arndtstrasse 22
D - 97072 Wuerzburg Germany
++49-931-7848700 Phone
++49-931-7848701 Fax
++49-175-7177051 Mobile

Websites:
www.nanoflight.info
www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
-----------------------------------------------------


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From: microscopy.listserver-at-gmail.com
Date: Sun, 16 Aug 2020 09:55:48 -0500
Subject: [Microscopy] viaWWW: Energy-dispersive x-ray quantification question

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X-from: johnbrad-at-hawaii.edu

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Email: johnbrad-at-hawaii.edu Name: John Bradley

Organization: HIGP University of Hawaii

Title-Subject: [Filtered] Energy-dispersive x-ray quantification

Message: When comparing Fe and Ni abundances within a suite of samples with identical crystal
structures, prepared in the same way and analyzed under the same conditions (including same total
counts in Fe and Ni):

1) Is the uncertainty associated with the Fe and Ni quantifications in each sample due solely to
peak intensities (total integrated counts)?

2) Does the same uncertainty apply to each of the samples.
Thanks and Aloha.

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From: microscopy.listserver-at-gmail.com
Date: Sun, 16 Aug 2020 13:42:12 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Energy-dispersive x-ray quantification

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X-from: Neal Magdefrau {semrental-at-gmail.com}



Hi John,
Are you using a standard less semi-quantitative routine or using standards? Most off the shelf EDS
software packages are using standard less and they use k-ratios of peak intensities along with
performing either ZAF or PhiRoZ corrections for absorption, fluoresce and atomic number. The
standard less routines are much less sensitive to changes in beam current and sample geometry
(working distance) compared to running pure elements standards. If you’re running pure standards
there are a lot of variables that will influence the uncertainty of the measurement including beam
current, working distance, sample flatness, etc.

Most EDS vendors running standardless account for beam current and working distance differences
which means for all things being equal it’s sometimes easier to get consistent uncertainties when
running a standard less quant routine vs. using elemental standards. At the end of the day it really
depends on what type of analysis you’re running and what specifically you’re looking for.

/*Thanks,*/
*/
/*
/*Neal Magdefrau, Ph.D*/
/*Electron Microscopy Innovative Tech.*/
/*(203) 978-3648*/
/*www.emitLLC.com*/

} On Aug 16, 2020, at 11:10 AM, microscopy.listserver-at-gmail.com wrote:
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} Title-Subject: [Filtered] Energy-dispersive x-ray quantification
}
} Message: When comparing Fe and Ni abundances within a suite of samples with identical crystal
} structures, prepared in the same way and analyzed under the same conditions (including same total
} counts in Fe and Ni):
}
} 1) Is the uncertainty associated with the Fe and Ni quantifications in each sample due solely to
} peak intensities (total integrated counts)?
}
} 2) Does the same uncertainty apply to each of the samples.
} Thanks and Aloha.
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From: microscopy.listserver-at-gmail.com
Date: Mon, 17 Aug 2020 07:45:49 -0500
Subject: [Microscopy] Fwd: AW: viaWWW: Energy-dispersive x-ray quantification

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X-from: Frank Eggert {frank.eggert-at-ametek.com}

Hi John,

actually I'm not 100% sure to have completely understood your question.
The uncertainty is determined by different points, one is the measured net-count counting
statistics. But these are total counts at peak area subtracted by background.
So another part of uncertainty is the background consideration, the used method (differs by
vendors). Just this becomes dominant with uncertainty if the concentration of the element is very small.
Keeping with net-count measurement, this value can vary due to systematic errors, e.g. sample Z
positioning or just you work with low magnification in SEM an select sample points once left top
corner and next then below right. The absolute detector-geometry will change due to this (solid
angle). And the beam-current is also a point of uncertainty (disappears at the end usually, if you
normalize the Quant results). This is with the counts.
If you mean finally the full quantitative results uncertainties (element concentrations), then
systematic error will be due to the Quant model and the used method (e.g. with standards or
standardless).
If you evaluate standardless, then you can assume the systematic error (uncertainty) with the model
will be actually same with all your similar samples.
This is then indeed this systematic uncertainty applies to each of the samples then.

Best regards
Frank Eggert

-----Ursprngliche Nachricht-----
Von: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Gesendet: Sonntag, 16. August
2020 17:01
An: Frank Eggert {frank.eggert-at-ametek.com}
Betreff: [Microscopy] viaWWW: Energy-dispersive x-ray quantification question

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Email: johnbrad-at-hawaii.edu Name: John Bradley

Organization: HIGP University of Hawaii

Title-Subject: [Filtered] Energy-dispersive x-ray quantification

Message: When comparing Fe and Ni abundances within a suite of samples with identical crystal
structures, prepared in the same way and analyzed under the same conditions (including same total
counts in Fe and Ni):

1) Is the uncertainty associated with the Fe and Ni quantifications in each sample due solely to
peak intensities (total integrated counts)?

2) Does the same uncertainty apply to each of the samples.
Thanks and Aloha.

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From: wesaia-at-iastate.edu
Date: Mon, 17 Aug 2020 08:57:18 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Energy-dispersive x-ray quantification

Contents Retrieved from Microscopy Listserver Archives
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I am not quite sure what the question is either. All we know about now is Fe and Ni. There was no mention of the rest of the sample other than it was consistent. What are the general levels of the elements? Is there anything that would be absorbing Ni more than Fe? What x-ray lines are being analyzed? What voltage was used?

Simply put, the precision has to do with the number of counts in the peak and background. For similar numbers for Fe and Ni, the precision should be the same. Of course, the background will be somewhat lower under Ni-K than Fe-K and the intensity of Ni will be less than Fe for the same mass fraction.

I would encourage you to get plenty of counts, period. We have many users in another lab that routinely fail to increase the beam current when doing x-ray analysis. Therefore, the uncertainties are much larger than they could be.

I have done plenty of standardless analysis and plenty of analyses with standards. I am not sure the differences are as much as Neal suggests. Differences in working distance and sample tilt (i.e., roughness) affect both types of analysis similarly. Beam current is usually an issue with standardized analyses since the results are often not normalized. Deviation of the total from 100% will reveal problems with the analysis. However, standardized analyses can be normalized (not necessarily advised) and the differences between the modes disappear.

Warren Straszheim, Ph.D., manager
Materials Analysis and Research Lab
23 Town Engineering
515-294-8187

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X-from: Neal Magdefrau {semrental-at-gmail.com}

Hi John,
Are you using a standard less semi-quantitative routine or using standards? Most off the shelf EDS software packages are using standard less and they use k-ratios of peak intensities along with performing either ZAF or PhiRoZ corrections for absorption, fluoresce and atomic number. The standard less routines are much less sensitive to changes in beam current and sample geometry (working distance) compared to running pure elements standards. If you’re running pure standards there are a lot of variables that will influence the uncertainty of the measurement including beam current, working distance, sample flatness, etc.

Most EDS vendors running standardless account for beam current and working distance differences which means for all things being equal it’s sometimes easier to get consistent uncertainties when running a standard less quant routine vs. using elemental standards. At the end of the day it really depends on what type of analysis you’re running and what specifically you’re looking for.

/*Thanks,*/
*/
/*
/*Neal Magdefrau, Ph.D*/
/*Electron Microscopy Innovative Tech.*/
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} On Aug 16, 2020, at 11:10 AM, microscopy.listserver-at-gmail.com wrote:
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} Title-Subject: [Filtered] Energy-dispersive x-ray quantification
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} Message: When comparing Fe and Ni abundances within a suite of samples
} with identical crystal structures, prepared in the same way and
} analyzed under the same conditions (including same total counts in Fe and Ni):
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} 1) Is the uncertainty associated with the Fe and Ni quantifications in
} each sample due solely to peak intensities (total integrated counts)?
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From: mdelann1-at-jhmi.edu
Date: Mon, 24 Aug 2020 13:49:02 -0500
Subject: [Microscopy] de humidifier

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Email: vau-at-ufl.edu Name: Karen Kelley

Organization: University of Florida

Title-Subject: [Filtered] HPF/FS archaea

Message: Does anyone have reference or methodology for HPF/FS of archaeal cells for enzyme detection
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From devlinrobb1unzwo-at-gmail.com Sat Aug 22 07:35:25 2020
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Hello all,
I wonder what other labs are doing to control the humidity in their Cryo EM experiments and scope operations. Are you dehumidifying the whole room with an expensive unit, or has someone figured out how to locally drop the humidity for prep and grid loading. Any comments are welcome.
Also what are your target humidity numbers.

Thanks,
Michael Delannoy


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From: microscopy.listserver-at-gmail.com
Date: Mon, 24 Aug 2020 16:44:25 -0500
Subject: [Microscopy] viaWWW:Job Opportunity - Electron Microscopy Lab Manager

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Email: david.evanoff-at-maine.edu Name: David Evanoff

Organization: University of Maine

Title-Subject: [Filtered] Job Opportunity - Electron Microscopy Lab Manager

Message: The University of Maine (Orono Campus) seeks qualified applicants for a Microscopy
Laboratory Manager. The centralized lab, which is open to any researcher at UMaine - Orono, as well
as external clients, has SEM, TEM, FIB, and various LM capabilities. Please see the link below for
more information and to apply.

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From: microscopy.listserver-at-gmail.com
Date: Tue, 25 Aug 2020 06:43:36 -0500
Subject: [Microscopy] viaWWW:Cryo-TEM & SPA training.

Contents Retrieved from Microscopy Listserver Archives
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X-from: ravithakkar-at-ksu.edu

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Email: ravithakkar-at-ksu.edu Name: Ravindra Thakkar

Organization: Kansas State University

Title-Subject: [Filtered] Cryo-TEM & SPA training.

Message: Hi,
I am pursuing my research in computational protein design. I have designed some inhibitory peptides
(17 residues long). I am looking for the institute where get the training for cryo-EM and SPA
method to validate the in-silico predicted peptide structures by the Cryo-EM SPA method. (I have an
expertise on TEM operation at room temperature mode)
It would be advantageous if I can get earn course credit hours for my Ph.D. degree through this course.
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From: microscopy.listserver-at-gmail.com
Date: Tue, 25 Aug 2020 06:44:45 -0500
Subject: [Microscopy] viaWWW: Grid holder for Jeol JEM1011

Contents Retrieved from Microscopy Listserver Archives
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X-from: nyilmaz-at-mersin.edu.tr

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Email: nyilmaz-at-mersin.edu.tr Name: sakir necat yilmaz
Organization: Mersin University

Title-Subject: [Filtered] Grid holder for Jeol JEM1011

Message: Dear All,

We have a broken pin on the grid holder of our Jeol JEM 1011. Please check the link below for
pictures. Do you have any idea to fix it or, can we find it as a spare part? Finally, we can happily
accept your unused, old holder as a gift :)))
Thanks in advance and best wishes from Turkey.


https://drive.google.com/drive/folders/1x_FWyWiyMQphBYH3dIddFbgoXdu0Mc7G?usp=sharing

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From: microscopy.listserver-at-gmail.com
Date: Thu, 27 Aug 2020 07:07:23 -0500
Subject: [Microscopy] Fwd: No signal on spectrometers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

We just published a manuscript analyzing the use of microscopy and the
reporting of imaging materials and methods in biomedical journals:
https://elifesciences.org/articles/55133
The punch line can be summarized in the title: Imaging methods are
vastly underreported in biomedical research
Slightly off topic, and arguably shameless self-promotion, but we
think the audience in this list is uniquely positioned to help solve
the problem.
Thanks for your interest.
Best,
Guillermo

Guillermo Marques
University Imaging Centers
University of Minnesota

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From klostocc091qove-at-gmail.com Wed Aug 26 08:05:29 2020
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X-from: Araz qolizadeh {araz79az-at-yahoo.com}


Hi all
I am working with EPMA Sx100, and unfortunately the Sp2,3,5 have no signals then we dont have
analysis,  these spectrometers stopped abruptly but not simultaneous, unfortunately, we did not
reach a conclusion during this period,
has anyone ever had such a problem and what could be the reason?
thanks
Kazem



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From: microscopy.listserver-at-gmail.com
Date: Thu, 27 Aug 2020 07:08:01 -0500
Subject: [Microscopy] viaWWW: Grid holder for Jeol JEM1011

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


X-from: Gilpin, Christopher J {gilpin-at-purdue.edu}

Tom Schmelzer at 724 453 3865 or tom-at-tgstechnologies.com is the go to for holder repairs. I am just
a satisfied customer.

​​​​​
Chris
He/Him/His
Christopher J. Gilpin Ph.D.
Campus-wide Coordinator for Electron Microscopy
Director, Life Science Microscopy Facility
Purdue University
Whistler Hall of Agriculture Research, Room S052
170 S. University St
West Lafayette, IN 47907
765-494-7750
gilpin-at-purdue.edu
lsmf-at-purdue.edu reaches everyone in the facility.
http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx
skype cjgilpin


-----Original Message-----
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2020 7:47 AM
To: Gilpin, Christopher J {gilpin-at-purdue.edu}

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Email: nyilmaz-at-mersin.edu.tr Name: sakir necat yilmaz
Organization: Mersin University

Title-Subject: [Filtered] Grid holder for Jeol JEM1011

Message: Dear All,

We have a broken pin on the grid holder of our Jeol JEM 1011. Please check the link below for
pictures. Do you have any idea to fix it or, can we find it as a spare part? Finally, we can happily
accept your unused, old holder as a gift :))) Thanks in advance and best wishes from Turkey.


https://drive.google.com/drive/folders/1x_FWyWiyMQphBYH3dIddFbgoXdu0Mc7G?usp=sharing

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From: microscopy.listserver-at-gmail.com
Date: Thu, 27 Aug 2020 07:08:40 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Grid holder for Jeol JEM1011

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X-from: John Shields {johnshields59-at-gmail.com}


Good morning Dr. Yilmaz,,

My only suggestion aside from finding a donor sample holder is to discover a good, strong,
non-gassing epoxy.
Or, if you are fortunate, a precision machine shop that could spot weld (very clean and precise) the
stub back in place.

We have a JEOL 1011, but unfortunately only one holder.  If you would like to use it in the future,
we have a YouTube video on holder removal and insertion that you could use as a training video.

https://youtu.be/cYDP4C4LpNs

You might reach out to JEOL. Their crew is very very helpful and can probably suggest solutions
other than purchasing another.

John Shields
Managing Director
Georgia Electron Microscopy

On Tue, Aug 25, 2020 at 7:57 AM {microscopy.listserver-at-gmail.com
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Email: nyilmaz-at-mersin.edu.tr {mailto:nyilmaz-at-mersin.edu.tr} Name: sakir necat yilmaz
Organization: Mersin University

Title-Subject: [Filtered] Grid holder for Jeol JEM1011

Message: Dear All,

We have a broken pin on the grid holder of our Jeol JEM 1011. Please check the link below for
pictures. Do you have any idea to fix it or, can we find it as a spare part? Finally, we can
happily
accept your unused, old holder as a gift :)))
Thanks in advance and best wishes from Turkey.


https://drive.google.com/drive/folders/1x_FWyWiyMQphBYH3dIddFbgoXdu0Mc7G?usp=sharing

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From: microscopy.listserver-at-gmail.com
Date: Thu, 27 Aug 2020 13:16:06 -0500
Subject: [Microscopy] Fwd: Re: Fwd: Re: viaWWW: Grid holder for Jeol JEM1011

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: V.Ray {vray-at-partbeamsystech.com}

This is just an alignment pin. Most likely its base is screwed in on thread, less likely but
possible - pressure fitted

In the threaded mating case - re-drill center of broken pin inside of the hole on a precision drill
press and use small screw extractor to remove it. Once removed - use small lathe to manufacture a
replacement pin from a suitable screw or even plain metal if you like cutting your own threads.
Aluminum bronze would be my first choice, but free-machining phosphor bronze, titanium, or even OFHC
copper should work in a pinch.

I the pressure fitting case - on a precision drill press drill out remains of the pin very closely
to walls of the hole and pry remains out with a sharp steel needle. Once the hole is cleaned, use
small lathe to make a replacement pin and drive it in with arbor press. Note that you would have to
place entire rod into suitable tube holder base to prevent any possible damage or deformation while
driving in a new pin.

I would try removing base of the pin with screw extractor first, and if it doesn't budge then assume
it is pressure fitting and proceed with close drilling.

Best of luck!
Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
www.partbeamsystech.com
www.fibsemproducts.com
www.freudlabs.com

"Only the Paranoid Survive" (A.Grove & SpaceX QA)

On 8/27/2020 8:08 AM, microscopy.listserver-at-gmail.com wrote:
} ----------------------------------------------------------------------------
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}
} X-from: John Shields {johnshields59-at-gmail.com}
}
}
} Good morning Dr. Yilmaz,,
}
} My only suggestion aside from finding a donor sample holder is to discover a good, strong,
} non-gassing epoxy.
} Or, if you are fortunate, a precision machine shop that could spot weld (very clean and precise) the
} stub back in place.
}
} We have a JEOL 1011, but unfortunately only one holder.  If you would like to use it in the future,
} we have a YouTube video on holder removal and insertion that you could use as a training video.
}
} https://youtu.be/cYDP4C4LpNs
}
} You might reach out to JEOL. Their crew is very very helpful and can probably suggest solutions
} other than purchasing another.
}
} John Shields
} Managing Director
} Georgia Electron Microscopy
}
} On Tue, Aug 25, 2020 at 7:57 AM {microscopy.listserver-at-gmail.com
} {mailto:microscopy.listserver-at-gmail.com} } wrote:
}
}
}
}
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} Email: nyilmaz-at-mersin.edu.tr {mailto:nyilmaz-at-mersin.edu.tr} Name: sakir necat yilmaz
} Organization: Mersin University
}
} Title-Subject: [Filtered] Grid holder for Jeol JEM1011
}
} Message: Dear All,
}
} We have a broken pin on the grid holder of our Jeol JEM 1011. Please check the link below for
} pictures. Do you have any idea to fix it or, can we find it as a spare part? Finally, we can
} happily
} accept your unused, old holder as a gift :)))
} Thanks in advance and best wishes from Turkey.
}
}
} https://drive.google.com/drive/folders/1x_FWyWiyMQphBYH3dIddFbgoXdu0Mc7G?usp=sharing
}
}   Login Host: 193.255.132.220
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From: microscopy.listserver-at-gmail.com
Date: Wed, 2 Sep 2020 07:05:30 -0500
Subject: [Microscopy] viaWWW: EELS & EFTEM Analysis Virtual Training School

Contents Retrieved from Microscopy Listserver Archives
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Email: jpatrick-at-morgridge.org Name: Jan Patrick

Organization: Morgridge Institute for Research

Title-Subject: [Filtered] Postdoctoral Research Associate

Message: A Postdoctoral Research Associate position is available in the newly established Grant
Laboratory within the Morgridge Institute for Research. The focus of the lab is the development of
methods for single-particle cryo electron microscopy (cryo-EM) and cryo electron tomography
(cryo-ET). The lab also develops the software package cisTEM (www.cistem.org), a user-friendly
package for processing cryo-EM data.
Requirements include: A Ph.D. in biology, biophysics, physics or a related field; experience in
image / signal processing; experience with cryo-EM data processing is desired; experience with
software programming. Ideally C++ programming and a strong publication record.

Qualified individuals interested in this opportunity are required to submit a cover letter and
resume as one document via the link below.

https://morgridge.org/job-posting/postdoctoral-research-associate/





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Email: stanvitha-at-tamu.edu Name: Stanislav Vitha

Organization: Texas A&M University

Title-Subject: [Filtered] Re: de-humidifier
Message: Hi Michael,
we ended up dehumidifying the TEM room only. The original set up was with the building A/C system
blowing the cold air under the floor (we have a 2 ft space between the floor and sub-floor) which
then rises through several perforated floor tiles and is then extracted through the air return
vents in the ceiling.
The relative humidity for most of the year is around 55% at 72F. This is determined by the fact that
the A/C system initially cools the air down to ~ 55 F, some water drops out and what is 100% RH at
55F is approximately 55% RH at 72F.
After a $35k engineering study and a ~$500k cost estimate we decided to try something less
costly first.

This was finished about 2 years ago.
I purchased two small desiccant wheel dehumidifiers, the model was Munters HD DryCool (no longer
available, but see my note below). They are a consumer model primarily for whole house
dehumidification, not an industrial/heavy duty model, but for the price it seemed a good option to
try. One important factor was its design, where they cool the incoming air and use the heat from it
to regenerate the desiccant (you need to blow hot air on the dessicant to regenerate it). The major
point is that the dehumidifiers do not heat up the processed air much (maybe 2-3 F), and thus the
building A/C system is not trying to pump more cold wet air into the room to keep the set temperature.

The company that I bought the dehumidifier from, BMIL (https://bmil.com/ - no commercial interest,
just a reasonably happy customer) was very helpful in running computer models to confirm the
dehumidification performance for my proposed setup, also provided advice on sound-dampening ducting,
ducting sizes, etc, ... So the overall solution was to intercept the incoming house air (typically
~55 to 65 F,), duct it to the service corridor next door where the dehumidifiers were installed, and
return the dry and still cold air back into the room (under the floor)
Because the incoming air flow is ~250 to 300 cfm, we needed to use two of the dehumidifiers, each
could only handle about 150 cfm, to get a theoretical reduction from ~55 % RH down to ~30% RH.

In practical life it works reasonably well. At least initially we were achieving about 35% RH on
normal hot humid Texas days. There may be some leaks of course, this is probably why we did not get
down to 30%, but overall the cryo-TEM (200 kV FEI Tecnai) seemed happier in terms of the vacuum
system and overall less ice buildup on cold surfaces of the holder during grid mounting and holder
insertion (Gatan 626 holder, now also Fichione holder).
All is not perfect, though, one of the dehumidifiers needed repair under warranty and then later the
same unit had the compressor die out of warranty and had to be fixed. The overall performance seems
to be drifting slowly towards higher humidity (currently 37 to 39 % RH on average). Some of it is
also because these days during training, the door is propped open to allow for social distancing
between the staff and the trainee in a relatively small room, or some users just do not care to keep
the door closed. I do not know for sure, I do not do cryo-EM myself I am just a guy who likes to
drill holes and construct things.
I have some pictures of our current setup, as well as 3D drawing of the design that I could send if
anybody is interested.

As I mentioned above, the Munters HD DryCool dehumidifiers are no longer available, but I was told
(by the same company where we bought them) that there is a larger unit from a different manufacturer
with 300 CFM capability. I wish we had this one when we did our project, it would have simplified
the ducting, having just one instead of two units.
The overall cost was about $10k for the two dehumidifiers, $1k for duct work inside the room (I
ran the ducts myself) $500 for a replacement back door of the room in which I cut holes for the
ducts, and about $20k to have the dehumidifiers professionally installed nextdoor - that included
building a hanging rack for the two units, running 4 x 50 ft of solid metal duct with insulation,
installing new electrical outlets, and ducting the hot wet regenerating air to the building exhaust
system.
I also spent another $350 for a networked Temp/humidity monitor so that myself and any of our users
can check on their computer or phone the current or past Temp and RH.
In a somewhat near future we will be doing room remodeling to accommodate a new cryo-FIB-SEM and
it will also involve moving, among other things, the cryo-plunging apparatus (for cryo-TEM samples)
into a new prep room where we may try reduce air humidity in a similar manner as I described above.
Stan

Dr. Stanislav Vitha
Microscopy and Imaging Center
Texas A&M University
ILSB 1131
College Station, TX 77843-2257

http://microscopy.tamu.edu

---------------------------------------------
Sent: Monday, August 24, 2020 1:55 PM

X-from: Lolita Rotkina {lolita.rotkina-at-yandex.com}



Hello to everybody,

I am looking for the tripod polisher. If anyone has it on the shelf collecting dust - I will be
happy to adopt it.
Temporary borrowing options are also in consideration.

Please, email me lolita.rotkina-at-Yandex.com

Thank you very much!

Lolita Rotkina,
Failure Analysis Engineer at TRUMPF Phitonics Inc.



Lolita Rotkina, PhD

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From johnsonloretta061-at-gmail.com Sat Aug 29 17:07:50 2020
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Email: john.hyun-at-ametek.com Name: John Hyun

Organization: Gatan
Title-Subject: [Filtered] EELS & EFTEM Analysis Virtual Training School

Message: EELS & EFTEM Analysis Virtual Training School - October 19-23, 2020
School fee: $250.00 USD

This is a virtual EELS School for all levels. The focus of the training will be live, interactive
microscope, and computer analysis sessions. The daily schedule for the 5-day school will include:

On-demand, pre-recorded lectures on key EELS and EFTEM topics.
Q&A break sessions: Live text and video chats with instructors and participants after main sessions.
Live microscope laboratories: Remote microscope sessions demonstrating next-generation EELS
imaging and spectroscopy systems and software. These sessions will be recorded.
Live software analysis laboratories: Live presentations, data analyses, and discussions. These
sessions will be recorded.
24/7 text and video chat channels with instructors and participants.
Complete training module: Lectures, laboratories, software demonstration software, sample data,
product literature, and EELS Atlas app provided as downloads.
Full access to the school virtual platform and materials during and 14 days after the school.

DESCRIPTION

Electron energy loss spectroscopy (EELS) is a powerful technique that provides both compositional
and chemical information from sub-nanometer areas in the sample. As a course attendee, you will
learn best practices to set up and optimize your EELS hardware and experimental protocols so you can
capture and extract the maximum amount of compositional and chemical information from your TEM
samples. Topics include:

Fundamentals of EELS and energy-filtered imaging in TEM
Principles of operation of EFTEM and EELS systems
Optimization of EFTEM and EELS data acquisition
Quantification of elemental composition
Other information provided by EFTEM/EELS and how best to extract it
Use of EELS signals to form maps of elemental and chemical composition
EFTEM and STEM EELS spectrum imaging techniques
Identification of material phases via EELS fine structure mapping
Applications to biological and physical science specimens
Registration:
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From: nizets2-at-yahoo.com
Date: Thu, 3 Sep 2020 03:52:49 -0500
Subject: [Microscopy] Question about peak masking in EDS + SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues!


We are having a discussion in the lab about a possible caveat/issue using EDS to element map a mineral sample in SEM.
Without disclosing too much about our project, I can say that the matrix is an alumosilicate which at one location is highly enriched in an element X.
When an element mapping (including line scan) is performed at this location, all we can see in EDS is element X, Al and Si almost disappeared.
Now the question is whether there really is no Al and Si at this location or whether they are present but cannot be detected because X is so enriched that it somehow hides Al and Si.


My opinion is that as long as the sensor is not saturated (deadtime } 0), Al and Si should be present in the spectrum, even though one may need to increase the dwell time significantly (except if X overlaps Si and/or Al but both is impossible I guess). My colleague reasoned using a "constant sum", which I don't know of, to explain why X can mask Si and Al.
I would need expert opinion on this matter.


If X can really mask Si and Al in EDS, would WDS offer a solution?


Thank you in advance.


Best regards,
Stephane


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From: james.quinn-at-stonybrook.edu
Date: Thu, Sep 3, 2020 at 5:01 AM
Subject: [Microscopy] Question about peak masking in EDS + SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Stephane,

Unless the peak from element X overlaps the Al & Si peaks, I would say that you should still see the matrix signal when the beam is next to the precipitate. Depending on the geometry of your system, if the generated X-rays pass through the precipitate on their way to the detector, there may be some significant absorption of Al & Si on one side of the precipitate.

If the precipitate is large enough, you have a reasonable dead time, and it is not showing Al & Si then I would say that those elements aren't in the phase. I'm not sure what your colleague means by "constant sum". The only thing I can think of is that, visually, if peak X grows quickly the Al & Si peaks shrink in comparison.

Cheers,
Henk

----------------


Hendrik O. Colijn
Center forElectronMicroscopy andAnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu


-----Original Message-----
X-from: nizets2-at-yahoo.com {nizets2-at-yahoo.com}
Sent: Thursday, September 3, 2020 4:56 AM
To: Colijn, Hendrik {colijn.1-at-osu.edu}

Stephane -

It would be nice to know the size of the location.

For instance, what if you had a 10um wide (and deep)
cube of MgO included in the matrix?

regards,
- Jim


---------- Forwarded message ---------
X-from: {nizets2-at-yahoo.com}

Dear colleagues!


We are having a discussion in the lab about a possible caveat/issue
using EDS to element map a mineral sample in SEM.
Without disclosing too much about our project, I can say that the
matrix is an alumosilicate which at one location is highly enriched in
an element X.
When an element mapping (including line scan) is performed at this
location, all we can see in EDS is element X, Al and Si almost
disappeared.
Now the question is whether there really is no Al and Si at this
location or whether they are present but cannot be detected because X
is so enriched that it somehow hides Al and Si.


My opinion is that as long as the sensor is not saturated (deadtime
} 0), Al and Si should be present in the spectrum, even though one may
need to increase the dwell time significantly (except if X overlaps Si
and/or Al but both is impossible I guess). My colleague reasoned using
a "constant sum", which I don't know of, to explain why X can mask Si
and Al.
I would need expert opinion on this matter.


If X can really mask Si and Al in EDS, would WDS offer a solution?


Thank you in advance.


Best regards,
Stephane

==============================Original Headers==============================
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From: wesaia-at-iastate.edu
Date: Thu, 3 Sep 2020 09:13:13 -0500
Subject: [Microscopy] Question about peak masking in EDS + SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

It's always hard to say much without full information. It would be nice to know what element X is and the rest of the composition. It might also help to know the EDS model involved and the instrumental conditions.

Mapping is not all that sensitive to changes in composition. Linescan is a little better, but spectra are the most sensitive to seeing small differences. I use mapping to reveal gross changes in chemistry. The noise per pixel obscures small changes, especially the way that I see lots of maps done.

You mention difficulties with mapping and you make a quick reference to linescan, but you did not say anything about regular spectra. Did you also collect them? That would provide a lot of information and might answer the questions. You did indicate if your sample does mapping with spectral imaging where all the spectrum is available at all points. If it did, then you could reconstruct the spectra for the phases and not go back and collect spectra specifically for the phases. I would really want to see the spectra. Is there something markedly different between the spectra? Is the Al and Si still present at a low level?

Suppose you did quant on the phases without normalization. Would the two areas give similar totals? If they did not, then something else is going on.

I appreciate Henk's comments in another reply. I also don't know what your colleague means by "constant sum". You have also said nothing about oxygen.


I think of two strange phenomena that have caused problems. I don't know if they might be at all related to your situation.

First, I remember someone giving a nice demonstration of the effect of count rate. I don't remember who it was, it has been many years, it might have been Chuck Fiore. The task was to map a copper TEM grid. They setup the SEM for brightness and count rate. They know they had to turn up the beam current from what was normally used for imaging so they setup for 30% deadtime using the entire field of view, maybe more. When they collected the map, they found Cu intensity in the holes of the grid and practically nothing on the bars of the grid. I should have said "Cu" intensity. It was the old school approach of mapping a region of interest without any background correction. When they were mapping the holes, they got some "Cu" intensity from the bremsstrahlung background. When they mapped the bars, the count rate shot up many times higher. If their input count rate was set for 20kcps over the entire grid, it probably jumped to 200 kcps on the bars and that effectively flooded the amplifier and shut it down so very few counts got out. So the holes had "Cu" and the grid bars had none.

Now I tried replicating this experience on an old Oxford ISIS, but I could not do it, per se. The ISIS corrected pixel dwell time for deadtime. So, if the deadtime went from 30 to 95%, my nominal dwell time of 10ms went from an actual dwell time of 14 ms to 200 ms (10ms*100%/(100%-95%). It gave the proper result, but the scanning was quite strange as the beam slowed to a crawl over the bars.

Second, I once tried analyzing a rare-earth phosphor that was severely cathodoluminescent. (Why should that be a surprise?) When the beam hit the phosphor, the count rate went through the roof. That meant dead time went to 100%. That shut down all output on a real-time basis. Even with dead-time correction, the analysis was impossible. In Oxford's defense, I cannot remember if this was their original detector or a replacement detector from Gresham. I do know that our current light-element detector is still subject to interference from light photons whether from the room or the IR camera in the SEM or from cathodoluminsence. Not knowing what element X is, I don't know if this might be relevant.

Warren Straszheim, Ph.D., manager
Materials Analysis and Research Lab
23 Town Engineering
515-294-8187


-----Original Message-----
X-from: nizets2-at-yahoo.com {nizets2-at-yahoo.com}
Sent: Thursday, September 03, 2020 3:54 AM
To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}

Dear colleagues!

We are having a discussion in the lab about a possible caveat/issue using EDS to element map a mineral sample in SEM.
Without disclosing too much about our project, I can say that the matrix is an alumosilicate which at one location is highly enriched in an element X.
When an element mapping (including line scan) is performed at this location, all we can see in EDS is element X, Al and Si almost disappeared.
Now the question is whether there really is no Al and Si at this location or whether they are present but cannot be detected because X is so enriched that it somehow hides Al and Si.

My opinion is that as long as the sensor is not saturated (deadtime } 0), Al and Si should be present in the spectrum, even though one may need to increase the dwell time significantly (except if X overlaps Si and/or Al but both is impossible I guess). My colleague reasoned using a "constant sum", which I don't know of, to explain why X can mask Si and Al.
I would need expert opinion on this matter.

If X can really mask Si and Al in EDS, would WDS offer a solution?

Thank you in advance.

Best regards,
Stephane

==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Tue, 8 Sep 2020 07:26:56 -0500
Subject: [Microscopy] viaWWW:biology EM use

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,
I would like to hear from any member who is proficient in unbiased design based stereological methods. I am looking for advice and help. Please contact privately.


Tom Bargar
Electron Microscopy Specialist
UNMC
Electron Microscopy Core Facility
986395 Nebraska Medical Center
Omaha, NE 68198-6395
tbargar-at-unmc.edu
402-559-7347


The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.


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Message: I want to recognize the ultrastructures or organelles labelled in hepatocyte of mouse. Dose
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From: microscopy.listserver-at-gmail.com
Date: Tue, 8 Sep 2020 07:27:36 -0500
Subject: [Microscopy] viaWWW:DI3000/3100 AFM for Donation

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Email: dcurley-at-vitro.com Name: Diane Curley

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Title-Subject: [Filtered] DI3000/3100 AFM for Donation

Message: DI3000/3100 Atomic Force Microscope by Digital/Veeco for donation. Microscope only, no
computer. Excellent working condition.

Located in Pittsburgh, PA
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From: microscopy.listserver-at-gmail.com
Date: Tue, 8 Sep 2020 07:28:16 -0500
Subject: [Microscopy] viaWWW:Dektak 8 Profilometer for Donation

Contents Retrieved from Microscopy Listserver Archives
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Email: dcurley-at-vitro.com Name: Diane Curley

Organization: Vitro Glass

Title-Subject: [Filtered] Dektak 8 Profilometer for Donation

Message: Dektak 8 Profilometer by Bruker for donation. Excellent working condition.

Located in Pittsburgh, PA
Covid-19 protocols in place for pickup

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From: microscopy.listserver-at-gmail.com
Date: Wed, 9 Sep 2020 17:20:19 -0500
Subject: [Microscopy] viaWWW: Loading nano magnetic particles onto TEM for imaging

Contents Retrieved from Microscopy Listserver Archives
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X-from: Dimitri Scholz {dimitri.scholz-at-ucd.ie}

Hi, can anyone perform a one-off job for my client with a He-microscope now?
It is about imaging pores in the material. They are really tiny: I could not detect any in my old
SEM. Should be smaller than 5 nm.
Cheers,
Dimitri

/Dimitri Scholz, PhD, Dr. rer. nat.
Director of Biological Imaging
Conway Institute
University College Dublin  UCD
Belfield, Dublin 4
Ireland
Mobile: +353-87-7961547


Electronic mail to, from, or within the University may be the subject of a request under the Freedom
of Information Acts 1997 and 2003/

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Email: fei.long-at-queensu.ca Name: Fei Long

Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for imaging

Message: Hi all,

I got a user want to do HRTEM on magnetite nanoparticles. My concern is that the nanoparticles will
be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and
contaminate the column. If anyone have similar experiences can provide advice which will be much
appreciated. The sample is powder which I usually dilute with alcohol then apply a drop of the
suspension onto a carbon coated grid.
Fei
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From: benada-at-biomed.cas.cz
Date: Thu, 10 Sep 2020 01:49:06 -0500
Subject: [Microscopy] Re: viaWWW: Loading nano magnetic particles onto TEM

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Hi Fei,
Some times ago, there was a discussion on this topic on the Microscopy
ListServer. I'm sorry but I don't remember when. Please go to the
Microscopy ListServer Archives and try to find it out:
{http://microscopy.com/MicroscopyListserver/MicroscopyArchives.html}

Regards

Oldrich

--
Oldřich Benada
Institute of Microbiology, Czech Acad. Sci.
Laboratory of Molecular Structure Characterization
Electron Microscopy Group
Vídeňská 1083
142 20 Prague 4
Czech Republic

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==============================Original Headers==============================
10, 29 -- From benada-at-biomed.cas.cz Thu Sep 10 01:49:03 2020
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10, 29 -- From: Oldrich Benada {benada-at-biomed.cas.cz}
10, 29 -- To: {microscopy-at-microscopy.com} , fee.long-at-queensu.ca
10, 29 -- Subject: Re: [Microscopy] viaWWW: Loading nano magnetic particles onto TEM
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From: wij.muss-at-aon.at
Date: Thu, 10 Sep 2020 08:23:30 -0500
Subject: [Microscopy] Re: Loading nano magnetic particles onto TEM in Re to O. BENADA's message, just for Fei's convenience

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The only "recent" Q/A via MSA-Listserver with regard to cleaning away
"magnetic particles" I am aware of

was notification sent:
Mon Mar 16 2020 17:34:02 How to clean nickel shim of magnetic and or
glass particles? Q by Nathan McCorkle

with two replies ( find text below only for your convenience)


Regards,


Wolfgang Muss
Retired MSA-Member
SALZBURG AUSTRIA


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~~~~~~~~
Q: From MSA-Listserver: Mon Mar 16 2020 17:34:02
I have a nickel shim destined for nanoimprint lithography, made by
electroforming e-beam exposed photoresist. I don't have a proper cleanroom,
but I've been trying to strip what seemed like residual ZEP e-beam resist...

And it has not been going so well. I've tried acetone, dichloromethane,
n-methyl pyrrolidone, and 10% NaOH.
Sonicated with heat in both acetone and NaOH (at different times). The NaOH
is the most recent attempt, and it seemed to show improvement under FIB
imaging, but I also noticed what appeared to be redeposition.

I can only imagine this is due to particulate in my solvents, dirty air as I
blow dry the shim or carry it from my sonicator to my FIB desk, or maybe
insoluble particles like glass or ferromagnetic dust which start to settle
onto the sample as soon as the sonicator is turned off.

Features are around 150nm linewidth, high frequency and complex shaped... So
lots of small say 500nm sized holes/crevices which I'd been thinking was
just diffusion limited for the solvent to get into and do it's work. But now
I'm pretty confused.

Should I invest in some .45 and .22 micron syringe filters for all my fluid
work?
Should I tape a magnet to the outside of the beaker I've been sonicating in,
to try and collect such particles?
What's a standard semiconductor lab method for cleaning magnetic particles
from magnetic layers?
How about the idea of insolubles?
Or can someone recommend a solution that will etch glass but not nickel?

Thanks,
-Nathan
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~~~~~~~~
Re (1) From MSA-Listserver: Sat Mar 21 08:47:00
Hi Nathan,
did you try using a plasma cleaner for cleaning the surfaces and also a
plasma cleaner like the Evactron at the FIB chamber to keep the specimen
clean during scanning?
If you can mount the specimen with the surface to be cleaned facing down to
the bottom of the beaker you might get rid of deposits coming from above ;-)
Another try to clean the surfaces might be to plunge it in liquid nitrogen
or to use a vacuum chamber with the cleaning solution and pump to a level
below sublimation.
And sure: clean micro-filtered solutions would help.
Nickel and magnetism: you could use a demagnetizer to decrease / erase the
magnetism in the shim first.

Best wishes,
Stefan
-----------------------------------------------------
Stefan Diller - Scientific Photography
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~~~~~~~~
Re (2): From MSA-Listserver: Sat Mar 21 08:47:00 2020
Hi Nathan,

To clean a surface of particulates, I would use replicating tape. This is a
cellulose acetate tape (non-adhesive) that you soften with acetone and press
down onto the surface. Let it dry and peel it off.
All the particulates should come with it. I've had better luck in removing
particulates this way compared with ultrasonics, rinses, etc.

I'm not sure if an adhesive tape will work but if you don't have replicating
tape, you might try some of the tape with the "Post-it" type adhesive.
It may take several applications to remove everything.

Replicating tape is available from most of the EM supply houses. It comes
in both a thick and thin form.

Cheers,
Henk
----------------
Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS The Ohio State University
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~~~~~~~~






-----Ursprngliche Nachricht-----
Von: benada-at-biomed.cas.cz [mailto:benada-at-biomed.cas.cz]
Gesendet: Donnerstag, 10. September 2020 08:50
An: wij.muss-at-aon.at
Betreff: [Microscopy] Re: Loading nano magnetic particles onto TEM

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Regards

Oldrich

--
Oldřich Benada
Institute of Microbiology, Czech Acad. Sci.
Laboratory of Molecular Structure Characterization Electron Microscopy Group
Vídeňská 1083
142 20 Prague 4
Czech Republic

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} Email: fei.long-at-queensu.ca Name: Fei Long
}
} Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for
} imaging
}
} Message: Hi all,
}
} I got a user want to do HRTEM on magnetite nanoparticles. My concern
} is that the nanoparticles will be pull away from the carbon coated
} grid by the strong magnetic filed of the TEM lens, and contaminate the
} column. If anyone have similar experiences can provide advice which
} will be much appreciated. The sample is powder which I usually dilute
} with alcohol then apply a drop of the suspension onto a carbon coated
} grid. Fei Login Host: 209.183.28.3
} Listserver Email Form V - 20120416
} ----------------------------------------------------------------------
} -----
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}
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From: microscopy.listserver-at-gmail.com
Date: Thu, 10 Sep 2020 08:41:53 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Loading nano magnetic particles onto

Contents Retrieved from Microscopy Listserver Archives
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X-from: Ravi Thakkar {ravi.thakkar369-at-gmail.com}



Hi Long,
You can use another carbon-coated grid on top of the grid loaded with magnetic nanoparticles.
With Thanks and Regards.
-------------------------------------------------------------------------
Ravindra Thakkar
Associate Scientist,
Kansas State University,
Manhattan, Kansas (USA)
-------------------------------------------------------------------------


On Wed, Sep 9, 2020 at 4:34 PM {microscopy.listserver-at-gmail.com
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Email: fei.long-at-queensu.ca {mailto:fei.long-at-queensu.ca} Name: Fei Long

Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for imaging

Message: Hi all,

I got a user want to do HRTEM on magnetite nanoparticles. My concern is that the nanoparticles will
be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and
contaminate the column. If anyone have similar experiences can provide advice which will be much
appreciated. The sample is powder which I usually dilute with alcohol then apply a drop of the
suspension onto a carbon coated grid.
Fei
  Login Host: 209.183.28.3
  Listserver Email Form V - 20120416
---------------------------------------------------------------------------


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From: microscopy.listserver-at-gmail.com
Date: Thu, 10 Sep 2020 08:42:32 -0500
Subject: [Microscopy] Fwd: RE: viaWWW: Loading nano magnetic particles onto

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X-from: Michel Ribardire {m.ribardiere-at-jeol.fr}



The emf is here if you have magneric field and movement.
So insert sample without magnetic field then use microscope with very slow movement.
For retracting sample use same way (no magnetic field)
Michel




Envoy de mon Galaxy A5 2017 Orange



-------- Message d'origine --------
De : microscopy.listserver-at-gmail.com
Date : 10/09/2020 00:30 (GMT+01:00)
: Michel Ribardire {m.ribardiere-at-jeol.fr}
Objet : [Microscopy] viaWWW: Loading nano magnetic particles onto TEM for imaging




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Email: fei.long-at-queensu.ca Name: Fei Long

Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for imaging

Message: Hi all,

I got a user want to do HRTEM on magnetite nanoparticles. My concern is that the nanoparticles will
be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and
contaminate the column. If anyone have similar experiences can provide advice which will be much
appreciated. The sample is powder which I usually dilute with alcohol then apply a drop of the
suspension onto a carbon coated grid.
Fei
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From: microscopy.listserver-at-gmail.com
Date: Thu, 10 Sep 2020 08:43:05 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: Loading nano magnetic particles onto

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X-from: Stephane Nizet {nizets2-at-yahoo.com}

Hi Fei!

This is indeed NOT a good idea, the particles WILL be captured by the lenses.
I am afraid the user needs to find another method.
An alternative may be to embed them and analyze sections? Be finding the right dilution, one should
be able to image only 1 isolated particle.

Regards,
Stephane







On Thursday, September 10, 2020, 01:26:44 AM GMT+2, microscopy.listserver-at-gmail.com
{microscopy.listserver-at-gmail.com} wrote:







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Email: fei.long-at-queensu.ca Name: Fei Long

Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for imaging

Message: Hi all,

I got a user want to do HRTEM on magnetite nanoparticles. My concern is that the nanoparticles will
be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and
contaminate the column. If anyone have similar experiences can provide advice which will be much
appreciated. The sample is powder which I usually dilute with alcohol then apply a drop of the
suspension onto a carbon coated grid.
Fei
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From: microscopy.listserver-at-gmail.com
Date: Thu, 10 Sep 2020 09:53:34 -0500
Subject: [Microscopy] viaWWW: Applications Scientist Position

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Email: marianne.mai-at-boeckeler.com Name: Marianne Mai

Title-Subject: [Filtered] Applications Scientist Position

Message: Ready for a change? Boeckeler Instruments is seeking an Applications Scientist to join our
team in Tucson, Arizona.

Key Responsibilities
Work closely with our Senior Applications Scientist to provide on-site and remote scientific
application support and training on sample preparation systems for electron microscopy and other
microscopies.
Install and train at customer sites when required.
Perform online or on-site product demonstrations.
Support the sales organization on customer application inquiries.
Conduct quality control testing on products.
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Qualifications
MS or PhD in a relevant life or physical science.
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may be interested. Thank you!

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From: matthew.weyland-at-monash.edu
Date: Thu, 10 Sep 2020 18:15:23 -0500
Subject: [Microscopy] Re: viaWWW: Loading nano magnetic particles onto TEM for

Contents Retrieved from Microscopy Listserver Archives
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Dear Fei

There seems to be some significant misunderstanding of the scale of
forces applied by the 'strong magnetic field'  inside a TEM. I've been
looking at magnetic nanoparticles of various kinds in every type of
TEM/STEM system for many years and never had any problems.

The van der waals forces - the electrostatics sticking your particles to
a grid are VASTLY stronger (several orders of magnitude) than the
magnetic moment for micro or nanoparticles inside a TEM. As long as you
are not dumping vast amounts of this material onto your grid (a good
rule of thumb is if you can see anything by eye in the suspension or on
the grid after deposition) you are generally safe. A good approach to be
really sure is to pass a rare earth magnet over the sample after
deposition to pick up any larger boulders/chunks (thanks Nestor).

The real risk for magnetic materials in TEM's is where a bulk specimens
can be become magnetised as a single or set of aligned domains (such as
a 3mm chunk of ferritic steel), then the combined magnetic moment can
tear a sample from a spring (jesus) clip. A safe holder to use in these
cases is always a screwed hexnut.

Best of luck,

Matthew

} X-from:fei.long-at-queensu.ca
}
} This Question/Comment was submitted to the Microscopy Listserver
} using the WWW based Form athttp://www.microscopy.com/MLFormMail.html
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} Email:fei.long-at-queensu.ca Name: Fei Long
}
} Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for imaging
}
} Message: Hi all,
}
} I got a user want to do HRTEM on magnetite nanoparticles. My concern is that the nanoparticles will
} be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and
} contaminate the column. If anyone have similar experiences can provide advice which will be much
} appreciated. The sample is powder which I usually dilute with alcohol then apply a drop of the
} suspension onto a carbon coated grid.
} Fei

--
Dr Matthew Weyland
Joint Deputy Director - Monash Centre for Electron Microscopy
Associate Professor - Department of Materials Science and Engineering

Monash Centre for Electron Microscopy
10 Innovation Walk, Clayton Campus
Monash University
Clayton VIC 3800 Australia

T: +61 3 9905 9026
E: matthew.weyland-at-monash.edu
mcem.monash.edu


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From: crwinkler-at-ncsu.edu
Date: Thu, 10 Sep 2020 19:55:28 -0500
Subject: [Microscopy] Re: viaWWW: Loading nano magnetic particles onto TEM for imaging

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Fei,

For a properly loaded TEM sample, the risk of nanoparticles being
ripped from the support is very low. By properly loaded, I mean that a
small amount of particles are evenly distributed across a TEM support
grid. You want to avoid clumps that are visible in a stereoscope or
microscope. Large clumps can be weakly adhered, or not adhered at all,
and could be pulled to one of the pole pieces. Your plan to dilute
with a solvent and drop onto a TEM grid is perfectly reasonable. Just
check it in an optical microscope to make sure you don't have any
clumps. If you do, dilute and repeat on a new grid.

I've looked at countless magnetite (and other magnetic NPs) and have
never observed any particles being removed from the support film. On
the TEMs I've used to image these samples, I've inspected the
objective pole pieces carefully during service and they were clean, at
least at the level of observation permitted by an optical microscope.
You can monitor the objective stigmator values over time to see if
you're getting gross contamination, but it would take a *very* large
number of NPs/clumps to make a perceivable impact. Now, bulk magnetic
samples are a very different story! I can tell you stories about
"hairy" pole pieces and magnetic samples making their home inside a
pole piece (I'm guilty of the latter, I'm afraid to admit).

Good luck,
Chris



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} Message: Hi all,
}
} I got a user want to do HRTEM on magnetite nanoparticles. My concern is that the nanoparticles will
} be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and
} contaminate the column. If anyone have similar experiences can provide advice which will be much
} appreciated. The sample is powder which I usually dilute with alcohol then apply a drop of the
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} Fei
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--
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From: microscopy.listserver-at-gmail.com
Date: Fri, 11 Sep 2020 21:28:54 -0500
Subject: [Microscopy] Fwd: Re: Fwd: Re: viaWWW: Loading nano magnetic

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X-from: Geiss,Roy {Roy.Geiss-at-colostate.edu}



I have done what Ravi suggests and it works fine. Just takes a little time to align the grid bars of
the two overlapping grids.

Roy Geiss
Research Staff
ARC-ISS
Colorado State University
Fort Collins, CO
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*To:* Geiss,Roy {Roy.Geiss-at-colostate.edu}
*Subject:* [Microscopy] Fwd: Re: viaWWW: Loading nano magnetic particles onto



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X-from: Ravi Thakkar {ravi.thakkar369-at-gmail.com}



Hi Long,
You can use anothercarbon-coated grid on top of the grid loaded with magnetic nanoparticles.
With Thanks and Regards.
-------------------------------------------------------------------------
Ravindra Thakkar
Associate Scientist,
Kansas StateUniversity,
Manhattan, Kansas (USA)
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On Wed, Sep 9, 2020 at 4:34 PM {microscopy.listserver-at-gmail.com
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Email: fei.long-at-queensu.ca {mailto:fei.long-at-queensu.ca} Name: Fei Long

Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for imaging

Message: Hi all,

I got a user want to do HRTEM on magnetite nanoparticles. My concern is that the
nanoparticles will
be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and
contaminate the column. If anyone have similar experiences can provide advice which will be much
appreciated. The sample is powder which I usually dilute with alcohol then apply a drop of the
suspension onto a carbon coated grid.
Fei
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From: crwinkler-at-ncsu.edu
Date: Wed, 16 Sep 2020 20:42:49 -0500
Subject: [Microscopy] Contrast enhancement strategies in TEM?

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Dear Colleagues,

The Stanford Nano Shared Facilities (SNSF) is seeking a Transmission Electron Microscopy (TEM) scientist to lead the operations of the facilities' Transmission Electron Microscopes, including the TF Titan and Tecnai TEMs. The TEM scientist will provide training and support to researchers, maintain and optimize the microscope operation, work closely with equipment vendors, maintain and develop training procedures, develop and implement advanced techniques, assist and advice users on specimen preparation and data analysis, provide proof-of-concept service, and engage in research activities. The SNSF Titan ETEM is equipped with a spherical aberration corrector, EELS and EDS, a monochromator and high brightness gun, Lorentz, holography, and tomography capabilities. The Tecnai TEM has a field-emission gun, STEM unit with bright field/dark field detector and EDS detectors. Both instruments are compatible with a full suite of in situ holders. The TEM scientist will work to utilize this range of advanced techniques to the highest extent, making them known to the user community, matching appropriate techniques to the individual research projects. S(he) will interact with the broader research community and equipment vendors to be aware of advances in the field, and make recommendations and prepare proposals for future equipment purchases. S(he) will promote and collaborate in the publication and presentation of results.

More details at: http://m.rfer.us/STANFORDr-HC2t

Thanks,

Tobi

Tobi Beetz, Ph.D.
Associate Director, Stanford Nano Shared Facilities, Stanford University, http://snsf.stanford.edu



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Email: helene_roberge-at-hotmail.fr {mailto:helene_roberge-at-hotmail.fr}
Name: Roberge Hélène

Title-Subject: [Filtered] Postdoctoral position offer IMN NANTES FRANCE - 3D Cryo FIB/SEM

Message: Hello !

A Postdoctoral Research position is available in the IMN Nantes laboratory within the University of
Nantes-FRANCE.The focus of the lab is the development of caracterisation methods for material
science. Here, this is about the development of cryos FIB/SEM microscopy techniques for caracterise
innovative  tissue  engineered  biomaterials  with stemcells encapsulated  inside  the biomaterials’
structure.

All the informations to apply are in the link :
https://emploi.cnrs.fr/Offres/CDD/UMR6502-PATABE-001/Default.aspx?lang=EN

Don't hesitate to share around you, the requierment include a good experience and skills in FIB/SEM
microscopy techniques, knowlegde on cryo FIB/SEM techniques is a nice advantage.

The team is cool, don't hesitate !

Thank you and have a nice day

Hélène Roberge
Phd student
IMN - Institut des Matériaux Jean Rouxel
2, rue de la Houssinière - BP 32229
44 322 NANTES CEDEX 3
https://www.cnrs-imn.fr/
0240376316

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From johnsonloretta061aihua-at-gmail.com Wed Sep 16 20:38:37 2020
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Message-ID: {8264F718.04DA2702-at-gmail.com}

Hello all,

We've recently moved to non-radioactive staining agents and are
finding that these alternatives generally offer less contrast than
their radioactive counterparts. In an effort to improve imaging of
these lower contrast materials, I've tried to think of every major way
to increase contrast from the TEM side of things. Our TEMs are
materials 'scopes and we don't have access to a dedicated bio TEM, so
I'm working with what I have.

I've moved to lower voltages, from 200 to 80kV, used as small of an
objective aperture as I can get away with, applied a generous amount
of defocus during imaging, and used the GIF to form images from the
elastic contribution.

Am I missing anything major besides digital manipulation of
brightness, contrast, and gamma on the images? Is it worth spending
the time and effort to align at 20kV to improve contrast in thin
sections?

Thanks for any advice you can share,
Chris

--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
https://www.aif.ncsu.edu/
Cell: 267-496-0587

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From: nizets2-at-yahoo.com
Date: Thu, 17 Sep 2020 06:47:25 -0500
Subject: [Microscopy] Viral particles quantification with EM

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues!

I was wondering if there would be a way to quantify (NOT detect!!) viral particles by EM (TEM or SEM).
Personally I cannot see one, the main probably being to assess the number of viral particles quantitatively.
Perhaps by SEM but the viral particles are approx. 100nm in diameter, which I couldn't resolve with our basic W-SEM.

Have somebody some experience with this topic?
Best regards,
Stephane

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From: microscopy.listserver-at-gmail.com
Date: Thu, 17 Sep 2020 07:33:09 -0500
Subject: [Microscopy] viaWWW: HT tank for our Transmission Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
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Chris,

I'm not sure what you mean by "GIF" in this case (I'm assuming you don't mean the file format), but if this isn't an aperture ... since you have materials 'scopes, you have a diffraction/field of view aperture. You can use this aperture to increase the contrast without any resolution loss (since this aperture isn't in the objective lens). It will give you a bit of resolution improvement by removing peripheral electrons, but the main negative effect is the vignetting. On our TEM, we can't use even the largest (200 µm) below ~10kX. The contrast increase is less than that from using the objective aperture, but it's noticeable.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office

-----Original Message-----
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Title-Subject: [Filtered] HT tank for our Transmission Electron Microscope

Message: Dear
We need to purchase the HT tank for our HRTEM (FEI, TF30, STWIN ) equipped with a Field Emission Gun
(FEG) gun and operating at 300 keV accelerating voltage. For this we need to repair/replace our
faulty HT tank under buyback scheme.
Please do needful if possible for you


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From: crwinkler-at-ncsu.edu
Date: Thu, 17 Sep 2020 08:53:29 -0500
Subject: [Microscopy] Re: [External] Contrast enhancement strategies in TEM?

Contents Retrieved from Microscopy Listserver Archives
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Hi Phil,

I guess I don't understand how the SA aperture increases contrast since it is in an image plane not a diffraction plane. You are referring to the SA aperture, right?

As a note... GIF is "Gatan Imaging Filter". Chris is trying to improve contrast by energy-filtering.

Cheers,
Henk

----------------


Hendrik O. Colijn
Center forElectronMicroscopy andAnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100

colijn.1-at-osu.edu 614/643-3458
cemas.osu.edu


-----Original Message-----
X-from: oshel1pe-at-cmich.edu {oshel1pe-at-cmich.edu}
Sent: Thursday, September 17, 2020 8:03 AM
To: Colijn, Hendrik {colijn.1-at-osu.edu}

Hello Phil,

I have the same question as Henk. Are you referring to the aperture
that is located at the intermediate crossover in JEOL microscopes? I
do miss that aperture because you could use it as a pseudo objective
to increase contrast in TEM mode, improve BF contrast in STEM mode,
and most usefully employ it during EDS. Or do you mean using the
selected area in low mag to improve contrast?

As Henk said, the GIF is the Gatan Energy Filter.

Thanks,
Chris

On Thu, Sep 17, 2020 at 8:07 AM Oshel, Philip Eugene {oshel1pe-at-cmich.edu} wrote:
}
} Chris,
}
} I'm not sure what you mean by "GIF" in this case (I'm assuming you don't mean the file format), but if this isn't an aperture ... since you have materials 'scopes, you have a diffraction/field of view aperture. You can use this aperture to increase the contrast without any resolution loss (since this aperture isn't in the objective lens). It will give you a bit of resolution improvement by removing peripheral electrons, but the main negative effect is the vignetting. On our TEM, we can't use even the largest (200 µm) below ~10kX. The contrast increase is less than that from using the objective aperture, but it's noticeable.
}
} Phil
} -------------
} Philip Oshel
} Imaging Facility Director
} Biology Department
} 1304 Biosciences
} 1455 Calumet Ct.
} Central Michigan University
} Mt. Pleasant, MI 48859
} 989 774-3576 office
}
} -----Original Message-----
} From: "crwinkler-at-ncsu.edu" {crwinkler-at-ncsu.edu}
} Reply-To: "crwinkler-at-ncsu.edu" {crwinkler-at-ncsu.edu}
} Date: Wednesday, 16September, 2020 at 21:57
} To: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu}
} Subject: [External] [Microscopy] Contrast enhancement strategies in TEM?
}
} Hello all,
}
} We've recently moved to non-radioactive staining agents and are
} finding that these alternatives generally offer less contrast than
} their radioactive counterparts. In an effort to improve imaging of
} these lower contrast materials, I've tried to think of every major way
} to increase contrast from the TEM side of things. Our TEMs are
} materials 'scopes and we don't have access to a dedicated bio TEM, so
} I'm working with what I have.
}
} I've moved to lower voltages, from 200 to 80kV, used as small of an
} objective aperture as I can get away with, applied a generous amount
} of defocus during imaging, and used the GIF to form images from the
} elastic contribution.
}
} Am I missing anything major besides digital manipulation of
} brightness, contrast, and gamma on the images? Is it worth spending
} the time and effort to align at 20kV to improve contrast in thin
} sections?
}
} Thanks for any advice you can share,
} Chris
}
} --
} Transmission Electron Microscopy Lab Manager
} Analytical Instrumentation Facility (AIF)
} NC State University
} https://www.aif.ncsu.edu/
} Cell: 267-496-0587
}
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--
Transmission Electron Microscopy Lab Manager
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Cell: 267-496-0587


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From: crwinkler-at-ncsu.edu
Date: Thu, 17 Sep 2020 09:57:11 -0500
Subject: [Microscopy] Re: Contrast enhancement strategies in TEM?

Contents Retrieved from Microscopy Listserver Archives
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Chris,

I mean the selected-area (SA) aka diffraction aka Hitachi's FOV (field-of-view) aperture. It increases contrast just because it blocks strays and peripheral electrons (like the fixed apertures do), and helps cut aberration a bit by blocking peripheral electrons. Just a side effect of being an aperture. Doesn't matter if it's in an image plane or a diffraction plane. Not a low mag aperture.
(I'd have to look at a column schematic to know if this is where the intermediate crossover is in a JEOL. It's below the objective lens, at the first intermediate in the Hitachi 7700.)
The effect isn't as strong as with an aperture specifically placed to increase contrast (in the objective lens), but it's still there.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office

-----Original Message-----
X-from: Christopher Winkler {crwinkler-at-ncsu.edu}

Hello Teresa,

Thank you for the paper and the protocol. I'll pass it along to the
scientist in charge to sample prep.

The idea about switching to STEM in the SEM is quite interesting.
Thank you! That's definitely something we will test out.

Cheers,
Chris


On Thu, Sep 17, 2020 at 10:26 AM Sawyer, Teresa
{sawyerte-at-science.oregonstate.edu} wrote:
}
} Hello Chris,
}
} OSU also only has an analytical TEM and it is difficult to image
} biologicals. Luck for me I can do STEM imaging on one of our SEM. With
} a larger field of view and the extra staining I do it works out great.
}
} I was introduced to O-T-O-T-O staining a few years back and now I use it
} all the time. You might not need to do all three osmium staining but
} this method has eliminated the UA or alternative from the protocol.
}
} I have attached the protocol but I have modified it some over the years.
} I cut the osmium staining time in half.
}
} I can not stress enough how much easier my life is with this staining
} and no more post microtome staining.
}
} Good luck
}
} Teresa
}
} On 9/16/2020 6:43 PM, crwinkler-at-ncsu.edu wrote:
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
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} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hello all,
} }
} } We've recently moved to non-radioactive staining agents and are
} } finding that these alternatives generally offer less contrast than
} } their radioactive counterparts. In an effort to improve imaging of
} } these lower contrast materials, I've tried to think of every major way
} } to increase contrast from the TEM side of things. Our TEMs are
} } materials 'scopes and we don't have access to a dedicated bio TEM, so
} } I'm working with what I have.
} }
} } I've moved to lower voltages, from 200 to 80kV, used as small of an
} } objective aperture as I can get away with, applied a generous amount
} } of defocus during imaging, and used the GIF to form images from the
} } elastic contribution.
} }
} } Am I missing anything major besides digital manipulation of
} } brightness, contrast, and gamma on the images? Is it worth spending
} } the time and effort to align at 20kV to improve contrast in thin
} } sections?
} }
} } Thanks for any advice you can share,
} } Chris
} }
} --
} Teresa Sawyer
} Electron Microscopy Facility Instrument Manager
} Oregon State University
} Linus Pauling Science Center 145
} 541-737-5245
} www.science.oregonstate.edu/emfacility
}


--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
https://www.aif.ncsu.edu/
Cell: 267-496-0587

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From: jehrman-at-mta.ca
Date: Thu, 17 Sep 2020 10:01:43 -0500
Subject: [Microscopy] Zeiss Primo Star binocular heads

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Listers,

The University bought 5 Zeiss Primo Star scopes for the Microbiology
lab, and wanted them all with trinocular heads. Inexplicably, Zeiss
shipped binocular heads with the scopes *and* the additional tinoculars.
So we have 5 brand new binocular heads that Zeiss doesn't want back. Can
anybody use them? The bean counters are willing to give them away (you
pay shipping) but if there's some sort of trade or money involved, I'll
look like a hero.

Not that I'm anticipating any takers. Most people want a trinoc as an
accessory, not the other way around.

Cheers,

Jim

--

James M. Ehrman
Digital Microscopy Facility
Mount Allison University
63B York St.
Sackville, NB E4L 1G7
CANADA

phone: 506-364-2519
fax: 506-364-2505
email: jehrman-at-mta.ca
www: www.mta.ca/dmf


==============================Original Headers==============================
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From: crwinkler-at-ncsu.edu
Date: Thu, 17 Sep 2020 10:13:29 -0500
Subject: [Microscopy] Contrast enhancement strategies in TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Henk,

STEM is definitely the next item on the menu. The trick about
inverting the HAADF image is a good one if you don't have a BF
detector!

I'm all too familiar with samples blowing up under the beam. A thin
coating of carbon on the exit surface (or sometimes both sides, in my
experience) usually solves it as you indicate. If only the carbon
coater was in the same building as the TEM...

Thanks for the advice,
Chris


On Thu, Sep 17, 2020 at 11:18 AM Colijn, Hendrik {colijn.1-at-osu.edu} wrote:
}
} Hi Chris,
}
} HAADF STEM will definitely allow you to increase contrast. However, most of the bio people choke when looking at a DF image. Since the bio samples have basically mass-thickness contrast, you should be able to invert the contrast and directly compare it with a std BF TEM image. You can play with the camera length to adjust the image.
}
} In STEM, the samples may start charging or blowing up if not carbon coated since the local charge buildup in the STEM probe can cause issues. A few nm of carbon takes care of the problem.
}
} Good luck,
} Henk
}
} ----------------
}
}
} Hendrik O. Colijn
} Center for Electron Microscopy and AnalysiS
} The Ohio State University
} 1305 Kinnear Rd, Suite 100
}
} colijn.1-at-osu.edu 614/643-3458
} cemas.osu.edu
}
}
} -----Original Message-----
} From: crwinkler-at-ncsu.edu {crwinkler-at-ncsu.edu}
} Sent: Thursday, September 17, 2020 11:00 AM
} To: Colijn, Hendrik {colijn.1-at-osu.edu}
} Subject: [Microscopy] Re: Contrast enhancement strategies in TEM?
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- https://urldefense.com/v3/__http://www.microscopy.com/MicroscopyListserver__;!!KGKeukY!igEIGfhUJ9v2_MZ1e8L5d_s2y_NT-NIeK6NUkt6sBSSFUj_jbiqukf6zvQVwexvM$
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} ----------------------------------------------------------------------------
}
} Hello Teresa,
}
} Thank you for the paper and the protocol. I'll pass it along to the scientist in charge to sample prep.
}
} The idea about switching to STEM in the SEM is quite interesting.
} Thank you! That's definitely something we will test out.
}
} Cheers,
} Chris
}
}
} On Thu, Sep 17, 2020 at 10:26 AM Sawyer, Teresa {sawyerte-at-science.oregonstate.edu} wrote:
} }
} } Hello Chris,
} }
} } OSU also only has an analytical TEM and it is difficult to image
} } biologicals. Luck for me I can do STEM imaging on one of our SEM.
} } With a larger field of view and the extra staining I do it works out great.
} }
} } I was introduced to O-T-O-T-O staining a few years back and now I use
} } it all the time. You might not need to do all three osmium staining
} } but this method has eliminated the UA or alternative from the protocol.
} }
} } I have attached the protocol but I have modified it some over the years.
} } I cut the osmium staining time in half.
} }
} } I can not stress enough how much easier my life is with this staining
} } and no more post microtome staining.
} }
} } Good luck
} }
} } Teresa
} }
} } On 9/16/2020 6:43 PM, crwinkler-at-ncsu.edu wrote:
} } } --------------------------------------------------------------------
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} } }
} } } Hello all,
} } }
} } } We've recently moved to non-radioactive staining agents and are
} } } finding that these alternatives generally offer less contrast than
} } } their radioactive counterparts. In an effort to improve imaging of
} } } these lower contrast materials, I've tried to think of every major
} } } way to increase contrast from the TEM side of things. Our TEMs are
} } } materials 'scopes and we don't have access to a dedicated bio TEM,
} } } so I'm working with what I have.
} } }
} } } I've moved to lower voltages, from 200 to 80kV, used as small of an
} } } objective aperture as I can get away with, applied a generous amount
} } } of defocus during imaging, and used the GIF to form images from the
} } } elastic contribution.
} } }
} } } Am I missing anything major besides digital manipulation of
} } } brightness, contrast, and gamma on the images? Is it worth spending
} } } the time and effort to align at 20kV to improve contrast in thin
} } } sections?
} } }
} } } Thanks for any advice you can share, Chris
} } }
} } --
} } Teresa Sawyer
} } Electron Microscopy Facility Instrument Manager Oregon State
} } University Linus Pauling Science Center 145
} } 541-737-5245
} } https://urldefense.com/v3/__http://www.science.oregonstate.edu/emfacil
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} }
}
}
} --
} Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://urldefense.com/v3/__https://www.aif.ncsu.edu/__;!!KGKeukY!igEIGfhUJ9v2_MZ1e8L5d_s2y_NT-NIeK6NUkt6sBSSFUj_jbiqukf6zvbKZeX3X$
} Cell: 267-496-0587
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} 8, 55 -- To: Microscopy-at-microscopy.com
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--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
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Cell: 267-496-0587


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From: microscopy.listserver-at-gmail.com
Date: Thu, 17 Sep 2020 17:48:10 -0500
Subject: [Microscopy] Fwd: Re: [External] Contrast enhancement strategies in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Erico Freitas {ericotadeu-at-ufmg.br}



Hi Henk,

I'm not sure about the kind of microscope Phil was referring to, but as for FEI microscopes such as
Tecnai the diffraction plane is at the selected aperture plane *only when in low mag mode (objective
lens off)*. So I guess in Phil's TEM it might be something similar.

Chris, I have used JEOL TEM a few times, so I am not quite familiar with. But, yes there might be a
way of switching off the objective less liki in low magnification mode. You will get a better contrast.

Another possibility that might work, as pointed out by Henk is to use the ADF or HAADF detector to
do dark field imaging and invert the image contrast. I've done (inverted contrast) ADF imaging on
non stained tissues and it works (with that sample I could see absolutely nothing in TEM mode).

Another thing you can think of is to do BSE-SEM imaging of the sectioned embedded block's surface.
Of course you need to carbon coat its surface before. Use the right SEM settings to reduce the
interaction volume. Maybe you can try lower kV, lower WD.

Good luck,

Erico





Em qui., 17 de set. de 2020 às 10:45, {colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} } escreveu:




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Hi Phil,

I guess I don't understand how the SA aperture increases contrast since it is in an image plane
not a diffraction plane.  You are referring to the SA aperture, right?

As a note... GIF is "Gatan Imaging Filter".  Chris is trying to improve contrast by
energy-filtering.

Cheers,
Henk

----------------


Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100

colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu}    614/643-3458
cemas.osu.edu {http://cemas.osu.edu}


-----Original Message-----
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{mailto:oshel1pe-at-cmich.edu} }
Sent: Thursday, September 17, 2020 8:03 AM
To: Colijn, Hendrik {colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} }
Subject: [Microscopy] Re: [External] Contrast enhancement strategies in TEM?




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Chris,

I'm not sure what you mean by "GIF" in this case (I'm assuming you don't mean the file format),
but if this isn't an aperture ... since you have materials 'scopes, you have a diffraction/field
of view aperture. You can use this aperture to increase the contrast without any resolution loss
(since this aperture isn't in the objective lens). It will give you a bit of resolution
improvement by removing peripheral electrons, but the main negative effect is the vignetting. On
our TEM, we can't use even the largest (200 µm) below ~10kX. The contrast increase is less than
that from using the objective aperture, but it's noticeable.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office

-----Original Message-----
X-from: "crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} " {crwinkler-at-ncsu.edu
{mailto:crwinkler-at-ncsu.edu} }
Reply-To: "crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} " {crwinkler-at-ncsu.edu
{mailto:crwinkler-at-ncsu.edu} }
Date: Wednesday,  16September, 2020 at 21:57
To: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }
Subject: [External] [Microscopy] Contrast enhancement strategies in TEM?

    Hello all,

    We've recently moved to non-radioactive staining agents and are
    finding that these alternatives generally offer less contrast than
    their radioactive counterparts. In an effort to improve imaging of
    these lower contrast materials, I've tried to think of every major way
    to increase contrast from the TEM side of things. Our TEMs are
    materials 'scopes and we don't have access to a dedicated bio TEM, so
    I'm working with what I have.

    I've moved to lower voltages, from 200 to 80kV, used as small of an
    objective aperture as I can get away with, applied a generous amount
    of defocus during imaging, and used the GIF to form images from the
    elastic contribution.

    Am I missing anything major besides digital manipulation of
    brightness, contrast, and gamma on the images? Is it worth spending
    the time and effort to align at 20kV to improve contrast in thin
    sections?

    Thanks for any advice you can share,
    Chris

    --
    Transmission Electron Microscopy Lab Manager
    Analytical Instrumentation Facility (AIF)
    NC State University

https://urldefense.com/v3/__https://www.aif.ncsu.edu/__;!!KGKeukY!jFW1hv1KxTkxRas5T101-Yd5jP024ydHnCLl-ddxiARaJ84iUFKXQOAQqf0ottp3$

    Cell: 267-496-0587



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From: microscopy.listserver-at-gmail.com
Date: Thu, 17 Sep 2020 17:48:45 -0500
Subject: [Microscopy] Fwd: Re: Contrast enhancement strategies in TEM?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Erico Freitas {ericotadeu-at-ufmg.br}


Hi Chris,


what TEM have you got?

It is a FEI/Thermofhisher machine you could try imaging your samples at low magnification (LM) mode.
In LM the objective lenses are off (you will see the value around 6% of it  strength), and in that
case the diffraction lenses act out as the objective lens (you may even want to place the selected
area aperture. In FEI machines you will have a better contrast in LM mode. I know the magnification
is not that high in LM mode, but you may still benefit from using your GIF camera that will give you
an extra magnification.

Best wishes

Em qua., 16 de set. de 2020 às 22:57, {crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} } escreveu:




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Hello all,

We've recently moved to non-radioactive staining agents and are
finding that these alternatives generally offer less contrast than
their radioactive counterparts. In an effort to improve imaging of
these lower contrast materials, I've tried to think of every major way
to increase contrast from the TEM side of things. Our TEMs are
materials 'scopes and we don't have access to a dedicated bio TEM, so
I'm working with what I have.

I've moved to lower voltages, from 200 to 80kV, used as small of an
objective aperture as I can get away with, applied a generous amount
of defocus during imaging, and used the GIF to form images from the
elastic contribution.

Am I missing anything major besides digital manipulation of
brightness, contrast, and gamma on the images? Is it worth spending
the time and effort to align at 20kV to improve contrast in thin
sections?

Thanks for any advice you can share,
Chris

-- Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
https://www.aif.ncsu.edu/
Cell: 267-496-0587

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--
Erico Freitas

Physicist / Microscopist / Lab Manager
Transmission Electron Microscopy for Materials Science laboratory
Center of Microscopy
Universidade Federal de Minas Gerais (UFMG)
Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901.
+55-31-3409-7573
+55-31-3409-7575

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From: benada-at-biomed.cas.cz
Date: Fri, 18 Sep 2020 01:31:56 -0500
Subject: [Microscopy] Re: Fwd: Re: [External] Contrast enhancement

Contents Retrieved from Microscopy Listserver Archives
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Hi Henk and Erico,
I can confirm BSE-SEM imaging can really be advantageous even on
ultrathin sections. A thin carbon coating is a must, as Erico mentioned
in his message. In BSE imaging mode one can play with accelerating
voltage, concentric back-scattered detector rings and beam deceleration
setting. With an optimal setting, you can image the really huge section
on TEM grid with almost no interference of TEM grid bars. Some years
ago we used such approaches on imaging of the whole ultrathin sections
through mice tooth. We were searching for places with Tomes’ processes
in ameloblasts and we were successful.

Regards Oldrich

--
Oldřich Benada
Institute of Microbiology, Czech Acad. Sci.
Laboratory of Molecular Structure Characterization
Electron Microscopy Group
Vídeňská 1083
142 20 Prague 4
Czech Republic

On Thu, 17 Sep 2020 17:48:37 -0500, microscopy.listserver-at-gmail.com
wrote :
}
}
}
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} X-from: Erico Freitas {ericotadeu-at-ufmg.br}
}
}
}
} Hi Henk,
}
} I'm not sure about the kind of microscope Phil was referring to, but
} as for FEI microscopes such as Tecnai the diffraction plane is at the
} selected aperture plane *only when in low mag mode (objective lens
} off)*. So I guess in Phil's TEM it might be something similar.
}
} Chris, I have used JEOL TEM a few times, so I am not quite familiar
} with. But, yes there might be a way of switching off the objective
} less liki in low magnification mode. You will get a better contrast.
}
} Another possibility that might work, as pointed out by Henk is to use
} the ADF or HAADF detector to do dark field imaging and invert the
} image contrast. I've done (inverted contrast) ADF imaging on non
} stained tissues and it works (with that sample I could see absolutely
} nothing in TEM mode).
}
} Another thing you can think of is to do BSE-SEM imaging of the
} sectioned embedded block's surface. Of course you need to carbon coat
} its surface before. Use the right SEM settings to reduce the
} interaction volume. Maybe you can try lower kV, lower WD.
}
} Good luck,
}
} Erico
}
}
}
}
}
} Em qui., 17 de set. de 2020 às 10:45, {colijn.1-at-osu.edu
} {mailto:colijn.1-at-osu.edu} } escreveu:
}
}
}
}
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} Hi Phil,
}
} I guess I don't understand how the SA aperture increases
} contrast since it is in an image plane not a diffraction plane.  You
} are referring to the SA aperture, right?
}
} As a note... GIF is "Gatan Imaging Filter".  Chris is trying to
} improve contrast by energy-filtering.
}
} Cheers,
} Henk
}
} ----------------
}
}
} Hendrik O. Colijn
} Center for Electron Microscopy and AnalysiS
} The Ohio State University
} 1305 Kinnear Rd, Suite 100
}
} colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu}    614/643-3458
} cemas.osu.edu {http://cemas.osu.edu}
}
}
} -----Original Message-----
} X-from: oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu}
} {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }
} Sent: Thursday, September 17, 2020 8:03 AM
} To: Colijn, Hendrik {colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} }
} Subject: [Microscopy] Re: [External] Contrast enhancement
} strategies in TEM?
}
}
}
}
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}
} Chris,
}
} I'm not sure what you mean by "GIF" in this case (I'm assuming
} you don't mean the file format), but if this isn't an aperture ...
} since you have materials 'scopes, you have a diffraction/field of
} view aperture. You can use this aperture to increase the contrast
} without any resolution loss (since this aperture isn't in the
} objective lens). It will give you a bit of resolution improvement by
} removing peripheral electrons, but the main negative effect is the
} vignetting. On our TEM, we can't use even the largest (200 µm) below
} ~10kX. The contrast increase is less than that from using the
} objective aperture, but it's noticeable.
}
} Phil
} -------------
} Philip Oshel
} Imaging Facility Director
} Biology Department
} 1304 Biosciences
} 1455 Calumet Ct.
} Central Michigan University
} Mt. Pleasant, MI 48859
} 989 774-3576 office
}
} -----Original Message-----
} X-from: "crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} "
} {crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} }
} Reply-To: "crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} "
} {crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} }
} Date: Wednesday,  16September, 2020 at 21:57
} To: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu
} {mailto:oshel1pe-at-cmich.edu} } Subject: [External] [Microscopy]
} Contrast enhancement strategies in TEM?
}
}     Hello all,
}
}     We've recently moved to non-radioactive staining agents and
} are finding that these alternatives generally offer less contrast than
}     their radioactive counterparts. In an effort to improve
} imaging of these lower contrast materials, I've tried to think of
} every major way to increase contrast from the TEM side of things. Our
} TEMs are materials 'scopes and we don't have access to a dedicated
} bio TEM, so I'm working with what I have.
}
}     I've moved to lower voltages, from 200 to 80kV, used as
} small of an objective aperture as I can get away with, applied a
} generous amount of defocus during imaging, and used the GIF to form
} images from the elastic contribution.
}
}     Am I missing anything major besides digital manipulation of
}     brightness, contrast, and gamma on the images? Is it worth
} spending the time and effort to align at 20kV to improve contrast in
} thin sections?
}
}     Thanks for any advice you can share,
}     Chris
}
}     --
}     Transmission Electron Microscopy Lab Manager
}     Analytical Instrumentation Facility (AIF)
}     NC State University
}
} https://urldefense.com/v3/__https://www.aif.ncsu.edu/__;!!KGKeukY!jFW1hv1KxTkxRas5T101-Yd5jP024ydHnCLl-ddxiARaJ84iUFKXQOAQqf0ottp3$
}
}     Cell: 267-496-0587
}
}
}
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From: crwinkler-at-ncsu.edu
Date: Fri, 18 Sep 2020 11:05:45 -0500
Subject: [Microscopy] Contrast enhancement in TEM - Summary of responses

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

Thank you to all for your responses and advice! I want to summarize
the responses I received regarding my query on how to improve contrast
on low contrast samples in a materials science TEM:

1) Buy a Bio TEM with a large gap objective optimized for high contrast. =D

2) Adjust staining protocols--check pH levels, switch to radioactive
stains (EH&S now makes this difficult in some universities),
supplement non-radioactive stains with lead citrate, tannic acid, or
other agents, and so forth. One responder linked me to the following
protocol that worked wonders for her:
https://www.sciencedirect.com/science/article/pii/S1047847714002378

3) Try to minimize contribution from the grid by moving to ultrathin
carbon or plasma cleaning thicker carbon to thin it down.

4) Acquire EFTEM SI and generate jump-ratio maps around the plasmons
peak, carbon peaks, etc.

5) Switch to STEM and see if contrast is improved when using ADF or BF
detectors. If you only have an ADF detector, invert the image contrast
to give a pseudo BF image (more acceptable to people used to TEM).
Similarly, switch to the SEM and try STEM or BSE imaging at 30kV.

6) Send a sample to Delong instruments and see how it looks at 5, 15,
and/or 25kV. Also, try working at lower voltages like 40 or 60kV.

7) Work in low mag (LM) and use the selected area aperture to further
improve contrast. LM contrast will be inherently higher as the
objective lens is turned nearly off.

8) Sum/stack several images to improve SNR. For thin sections with
little contrast and no outstanding features for correlation, this will
be tricky.

9) Replace one of the objective apertures with a phase plate.

10) For JEOL and Hitachi microscopes, use the field limiting aperture
located below the objective to improve contrast.

Thanks again to everyone for all the help. We'll be trying STEM in the
TEM and SEM next.

Thanks,
Chris




--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
https://www.aif.ncsu.edu/
Cell: 267-496-0587

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From: oshel1pe-at-cmich.edu
Date: Fri, 18 Sep 2020 14:35:41 -0500
Subject: [Microscopy] Fwd: Re: [External] Contrast enhancement strategies in

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I have a Hitachi 7700. The FOV (field-of-view), aka selected area aperture, works at all mags, not just in low mag. I suspect the same is true for any TEM with a SA (selected area - let's expand the abbreviations in the emails). Just do your imaging as normal, and if you need a contrast bump without losing resolution, put in the SA/FOV aperture. It's just adding another aperture, not changing any imaging parameters or any electronics. You do lose field of view at mags {10,000 or so, depending on the aperture size, but that's it.
Assuming you have a manually variable aperture and not some computer-controlled thing that only works when and how the computer thinks it should.
If that doesn't exist, it's coming.

Phil
-----------------------------------------
Philip Oshel
Imaging Center Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576 office​
(989) 774-7567 lab



________________________________________
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com}
Sent: Thursday, September 17, 2020 18:54
To: Oshel, Philip Eugene

X-from: Erico Freitas {ericotadeu-at-ufmg.br}



Hi Henk,

I'm not sure about the kind of microscope Phil was referring to, but as for FEI microscopes such as
Tecnai the diffraction plane is at the selected aperture plane *only when in low mag mode (objective
lens off)*. So I guess in Phil's TEM it might be something similar.

Chris, I have used JEOL TEM a few times, so I am not quite familiar with. But, yes there might be a
way of switching off the objective less liki in low magnification mode. You will get a better contrast.

Another possibility that might work, as pointed out by Henk is to use the ADF or HAADF detector to
do dark field imaging and invert the image contrast. I've done (inverted contrast) ADF imaging on
non stained tissues and it works (with that sample I could see absolutely nothing in TEM mode).

Another thing you can think of is to do BSE-SEM imaging of the sectioned embedded block's surface.
Of course you need to carbon coat its surface before. Use the right SEM settings to reduce the
interaction volume. Maybe you can try lower kV, lower WD.

Good luck,

Erico





Em qui., 17 de set. de 2020 às 10:45, {colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} } escreveu:




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Hi Phil,

I guess I don't understand how the SA aperture increases contrast since it is in an image plane
not a diffraction plane. You are referring to the SA aperture, right?

As a note... GIF is "Gatan Imaging Filter". Chris is trying to improve contrast by
energy-filtering.

Cheers,
Henk

----------------


Hendrik O. Colijn
Center for Electron Microscopy and AnalysiS
The Ohio State University
1305 Kinnear Rd, Suite 100

colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} 614/643-3458
cemas.osu.edu {http://cemas.osu.edu}


-----Original Message-----
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Sent: Thursday, September 17, 2020 8:03 AM
To: Colijn, Hendrik {colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} }
Subject: [Microscopy] Re: [External] Contrast enhancement strategies in TEM?




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Chris,

I'm not sure what you mean by "GIF" in this case (I'm assuming you don't mean the file format),
but if this isn't an aperture ... since you have materials 'scopes, you have a diffraction/field
of view aperture. You can use this aperture to increase the contrast without any resolution loss
(since this aperture isn't in the objective lens). It will give you a bit of resolution
improvement by removing peripheral electrons, but the main negative effect is the vignetting. On
our TEM, we can't use even the largest (200 µm) below ~10kX. The contrast increase is less than
that from using the objective aperture, but it's noticeable.

Phil
-------------
Philip Oshel
Imaging Facility Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
989 774-3576 office

-----Original Message-----
X-from: "crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} " {crwinkler-at-ncsu.edu
{mailto:crwinkler-at-ncsu.edu} }
Reply-To: "crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} " {crwinkler-at-ncsu.edu
{mailto:crwinkler-at-ncsu.edu} }
Date: Wednesday, 16September, 2020 at 21:57
To: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }
Subject: [External] [Microscopy] Contrast enhancement strategies in TEM?

Hello all,

We've recently moved to non-radioactive staining agents and are
finding that these alternatives generally offer less contrast than
their radioactive counterparts. In an effort to improve imaging of
these lower contrast materials, I've tried to think of every major way
to increase contrast from the TEM side of things. Our TEMs are
materials 'scopes and we don't have access to a dedicated bio TEM, so
I'm working with what I have.

I've moved to lower voltages, from 200 to 80kV, used as small of an
objective aperture as I can get away with, applied a generous amount
of defocus during imaging, and used the GIF to form images from the
elastic contribution.

Am I missing anything major besides digital manipulation of
brightness, contrast, and gamma on the images? Is it worth spending
the time and effort to align at 20kV to improve contrast in thin
sections?

Thanks for any advice you can share,
Chris

--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University

https://urldefense.com/v3/__https://www.aif.ncsu.edu/__;!!KGKeukY!jFW1hv1KxTkxRas5T101-Yd5jP024ydHnCLl-ddxiARaJ84iUFKXQOAQqf0ottp3$

Cell: 267-496-0587



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From: microscopy.listserver-at-gmail.com
Date: Sat, 19 Sep 2020 16:05:32 -0500
Subject: [Microscopy] viaWWW: TEM fungi

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Message: Does anyone have a protocol for embedding fungi?
thanks
sue

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From: microscopy.listserver-at-gmail.com
Date: Sun, 20 Sep 2020 07:59:23 -0500
Subject: [Microscopy] viaWWW: TEM fungi

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X-from: Czymmek, Kirk {KCzymmek-at-danforthcenter.org}

Hi Sue,

Any specific fungal structures you are targeting? Hyphae, conidia, yeast? Conventional fixation or cryo?



Best Regards, Kirk


Kirk J Czymmek, Ph.D.
Director, Advanced Bioimaging Laboratory
Principal Investigator
Donald Danforth Plant Science Center
975 North Warson Road|Saint Louis, Missouri 63132
t:314.587.1261 | c:203.833.3476 |f:314.587.1503 | e:kczymmek-at-danforthcenter.org
www.danforthcenter.org


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thanks
sue

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From: microscopy.listserver-at-gmail.com
Date: Sun, 20 Sep 2020 07:59:48 -0500
Subject: [Microscopy] Fwd: Re: viaWWW: TEM fungi

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X-from: Vad Perez {vad.perez-at-gmail.com}

Hi, Sue
You may want to contact the CICESE branch of the National Laboratory of Advanced Microscopy
https://www.facebook.com/pg/LNMA.CICESE/about/
They are very skilled in EM of fungi.
Cheers
Vad

On Sat, Sep 19, 2020 at 4:17 PM {microscopy.listserver-at-gmail.com
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Email: susan.vanhorn-at-stonybrook.edu {mailto:susan.vanhorn-at-stonybrook.edu} Name: Susan C Van Horn

Title-Subject: [Filtered] TEM fungi

Message: Does anyone have a protocol for embedding fungi?
thanks
sue

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From: microscopy.listserver-at-gmail.com
Date: Mon, 21 Sep 2020 07:34:23 -0500
Subject: [Microscopy] Fwd: Re: Fwd: RE: viaWWW: TEM fungi

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X-from: Susan Van Horn {susan.vanhorn-at-stonybrook.edu}

Hi - hyphae and normal fixation........thanks for quick reply
sue

On Sun, Sep 20, 2020 at 9:25 AM {microscopy.listserver-at-gmail.com

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X-from: Czymmek, Kirk {KCzymmek-at-danforthcenter.org {mailto:KCzymmek-at-danforthcenter.org} }

Hi Sue,

Any specific fungal structures you are targeting? Hyphae, conidia, yeast? Conventional fixation
or cryo?



Best Regards, Kirk


Kirk J Czymmek, Ph.D.
Director, Advanced Bioimaging Laboratory
Principal Investigator
Donald Danforth Plant Science Center
975 North Warson Road|Saint Louis, Missouri 63132
t:314.587.1261 | c:203.833.3476 |f:314.587.1503 | e:kczymmek-at-danforthcenter.org
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Subject: [Microscopy] viaWWW: TEM fungi




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Email: susan.vanhorn-at-stonybrook.edu {mailto:susan.vanhorn-at-stonybrook.edu} Name: Susan C Van Horn

Title-Subject: [Filtered] TEM fungi

Message: Does anyone have a protocol for embedding fungi?
thanks
sue

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***************************************************************
Susan C. Van Horn, M.A.
C-MIC TEM Facility
Office of Scientific Affairs
Life Sciences Building
Room 046
SUNY-at-Stony Brook
Stony Brook, NY 11794-5200
phone: 631-632-8623
     fax: 631-632-7728
email: susan.vanhorn-at-stonybrook.edu {mailto:susan.vanhorn-at-sunysb.edu}
Central Microscopy Imaging Center (C-MIC) website:
https://osa.stonybrookmedicine.edu/research-core-facilities/microscopy/services


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From: microscopy.listserver-at-gmail.com
Date: Wed, 23 Sep 2020 15:29:01 -0500
Subject: [Microscopy] Fwd: Morniroli Electron Diffraction Software on Windows 10

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


X-from: Czymmek, Kirk {KCzymmek-at-danforthcenter.org}

Hi Sue,
While Embed812 and similar epoxy resins can work, in general I like to use graded acetone series and
Quetol 651 for fungi (and plants) as it has good contrast and its low viscosity help reduce the
possibility of infiltration problems due to the fungal cell wall.

I will reach out to you directly with more detailed protocol.

Best Regards, Kirk


Kirk J Czymmek, Ph.D.
Director, Advanced Bioimaging Laboratory
Principal Investigator
Donald Danforth Plant Science Center
975 North Warson Road|Saint Louis, Missouri 63132
t:314.587.1261 | c:203.833.3476 |f:314.587.1503 | e:kczymmek-at-danforthcenter.org
www.danforthcenter.org


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Monday, September 21,
2020 7:42 AM
To: Czymmek, Kirk {KCzymmek-at-danforthcenter.org}


X-from: Susan Van Horn {susan.vanhorn-at-stonybrook.edu}

Hi - hyphae and normal fixation........thanks for quick reply sue

On Sun, Sep 20, 2020 at 9:25 AM {microscopy.listserver-at-gmail.com

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X-from: Czymmek, Kirk {KCzymmek-at-danforthcenter.org {mailto:KCzymmek-at-danforthcenter.org} }

Hi Sue,

Any specific fungal structures you are targeting? Hyphae, conidia, yeast? Conventional fixation
or cryo?



Best Regards, Kirk


Kirk J Czymmek, Ph.D.
Director, Advanced Bioimaging Laboratory
Principal Investigator
Donald Danforth Plant Science Center
975 North Warson Road|Saint Louis, Missouri 63132
t:314.587.1261 | c:203.833.3476 |f:314.587.1503 | e:kczymmek-at-danforthcenter.org
{mailto:e%3Akczymmek-at-danforthcenter.org}
www.danforthcenter.org {http://www.danforthcenter.org}


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com}
{microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } Sent: Saturday,
September
19, 2020 4:14 PM
To: Czymmek, Kirk {KCzymmek-at-danforthcenter.org {mailto:KCzymmek-at-danforthcenter.org} }
Subject: [Microscopy] viaWWW: TEM fungi




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Email: susan.vanhorn-at-stonybrook.edu {mailto:susan.vanhorn-at-stonybrook.edu} Name: Susan C Van Horn

Title-Subject: [Filtered] TEM fungi

Message: Does anyone have a protocol for embedding fungi?
thanks
sue

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--

***************************************************************
Susan C. Van Horn, M.A.
C-MIC TEM Facility
Office of Scientific Affairs
Life Sciences Building
Room 046
SUNY-at-Stony Brook
Stony Brook, NY 11794-5200
phone: 631-632-8623
     fax: 631-632-7728
email: susan.vanhorn-at-stonybrook.edu {mailto:susan.vanhorn-at-sunysb.edu}
Central Microscopy Imaging Center (C-MIC) website:
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Does anyone know how to install the Morniroli Electron Diffraction Software on Windows 10 Pro?

-Scott Walck


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From: microscopy.listserver-at-gmail.com
Date: Thu, 24 Sep 2020 13:22:36 -0500
Subject: [Microscopy] viaWWW:Flucam saturation on Themis Z

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Email: eric.gautron-at-cnrs-imn.fr Name: Eric Gautron

Title-Subject: [Filtered] Flucam saturation on Themis Z

Message: Hi all ThemisZ users,
since the upgrade of the main TEM software to 2.15.3, the flucam saturates and the monochromator
stigmators tuning with Optimono fails (probably due to that saturation). Despite remote and on-site
support and a flucam exchange, the situation is still the same than two months ago.
Does anybody have the same problem ? Best regards,
Eric

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From: microscopy.listserver-at-gmail.com
Date: Thu, 24 Sep 2020 13:23:18 -0500
Subject: [Microscopy] viaWWW: Immediate job opening - research associate

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Email: cni-at-udel.edu Name: Chaoying Ni

Title-Subject: [Filtered] Immediate job opening - research associate

Message: University of Delaware has an immediate opening for a post-doctoral research associate
ideally with any of TEM/SEM/AFM experience in physical science. The successful candidate may become
a permanent staff down the load as the campus life becomes more normal.
For more information and application, please check out the site below:

https://careers.udel.edu/cw/en-us/job/496152/post-doctoral-research-associate-electron-microscopy

For inquiries, please send email to cni-at-udel.edu
Thanks!

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From: microscopy.listserver-at-gmail.com
Date: Fri, 25 Sep 2020 06:11:55 -0500
Subject: [Microscopy] viaWWW:TEM Research Associate position at NIH

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Email: yaroslav.tsybovsky-at-nih.gov Name: Yaroslav Tsybovsky

Title-Subject: [Filtered] TEM Research Associate position at NIH

Message: Dear colleagues,

We have an open Research Associate position at the Frederick National Laboratory for Cancer Research
in Frederick, Maryland. The primary responsibilities will be to prepare samples (mostly proteins)
for EM imaging by negative staining and plunge freezing and to collect data using a range of
electron microscopes, with opportunities to perform other tasks such as computational processing.
Traditional EM (plastic embedding/sectioning) will be performed on occasion. This position requires
a bachelors degree. Proficiency in the use of transmission electron microscopes and desire to learn
will be of high value.
Please use the following link to see the details and apply:
https://leidosbiomed.csod.com/ats/careersite/jobdetails.aspx?site=4&c=leidosbiomed&id=1380


Yaroslav Tsybovsky, Ph.D. (contractor)
Scientist II
Frederick National Laboratory for Cancer Research
Leidos Biomedical Research, Inc. Post Office Box B Frederick, Maryland 21702 Phone: 301-846-7550

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From: microscopy.listserver-at-gmail.com
Date: Fri, 25 Sep 2020 16:39:32 -0500
Subject: [Microscopy] viaWWW:software script allowing the automatic focusing of the samples

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Email: sobol-at-img.cas.cz Name: Margarita Sobol

Title-Subject: [Filtered] the software script allowing the automatic focusing of the samples during
the EM high-throughput acquisition

Message: I use Jeol 1400 Flash EM, which has a montage system using the stage drive to shift the
field of view (Limitless Panorama). This system enables a montage panorama image to be captured over
a wide area. Actually, I acquire the grid squares one by one and I always focus each grid square
separately. Also I check if the focus is OK for each corner and the center of the grid square.
Usually, it is so when the acquisition starts, but after the first hour (LLP lasts 6-8 hours for the
grid square at x30K) the image becomes to be out of focus and I need to pause the capturing and
adjust the focus manually. This means that the overnight LLP is often useless for the analysis.
I need the software script which allows the automatic focusing of the samples during the EM
high-throughput acquisition
Thanks a lot in advance!

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From: microscopy.listserver-at-gmail.com
Date: Sat, 26 Sep 2020 06:23:18 -0500
Subject: [Microscopy] viaWWW: Sales Manager USA Position -Boeckeler Instruments

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Email: sayitugurlu-at-gmail.com Name: Sayit Uğurlu

Title-Subject: [Filtered] New type of EDS detector

Message: I have an idea about creating a new type of x-ray detector for scanning electron
microscopy. Unfortunately, I cannot give much information yet but here is some part I can share
without rewiring how the detector works. The detector itself is actually not very complex. It is
based on some foundementers physic laws. I am expecting that it would have some unique properties
like detecting very low traces of elements and detecting soft x-ray and UV.It also would be a new
technology so I am hoping that It would be patent-able. So I am just asking 45 min. to listen to
me,If there anyone willingly investigate it.
B.R.
Sayit Uğurlu

Abstract: The purpose of this project is creating a new kind of detector for X-ray characterization.
Each current technology has some level of disadvantage. The Windowless EDS has low resolution and
hard to detect low energy x-ray. The WDS is expensive. This detector design for detecting very low
energy x-ray and getting better resolution. Also, It would be the cheapest x-ray spectrometer in the
market.

In theory it can;
-detect Li and other soft X-ray
-It is cheap. Like 7000-8000 euro total cost.
-It is hard to say something about resolution. If you can wait you can detect very low % of
elements Like %0,01.

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From trojlita384ahuwi-at-gmail.com Fri Sep 25 23:20:26 2020
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Email: marianne.mai-at-boeckeler.com Name: Marianne Mai

Title-Subject: [Filtered] Sales Manager USA Position

Message: Boeckeler Instruments is seeking a Sales Manager for the USA region to develop and manage
customer and dealer relationships for our line of ultramicrotomy products and services.
Key Responsibilities

Drive growth across sales channels (direct and through intermediaries)
Develop and implement sales plans and forecasts to achieve corporate objectives for products and
services
Oversee and evaluate market research and adjust sales/marketing strategy to meet changing market
and competitive conditions
Establish and maintain relationships with industry influencers and key strategic partners
Direct market channel development activity and coordinate sales distribution by establishing sales
territories, quotas and goals
Represent company at trade association meetings, workshops and other events
Organize online or onsite workshops, demos and other customer related events
Meet with key clients and intermediaries to maintain relationships, negotiate and close deals
Install and train at customer sites when required
Prepare periodic sales report showing sales volume, potential sales, and areas of proposed client
base expansion
Review and analyze sales performances against programs, quotes and plans to determine effectiveness
Collaborate with Applications and R&D teams on market feedback for the development of new solutions
Qualifications

Bachelors degree, Masters degree or PhD in Engineering, Biology or related field
Minimum of three years or more in managing dealer and or end-user relationships Experience in
engineering, life science or materials science research
Experience in various microscopy techniques is highly desired Have excellent people skills
Able to travel regularly
Based in or willing to relocate to Tucson, AZ

Please send your CV to careers-at-boeckeler.com

About Boeckeler Instruments
Boeckeler Instruments, Inc. is a privately-owned company that engineers sample preparation equipment
for nanoscale research, under the RMC Boeckeler product line. Our solutions are employed in a broad
range of areas including materials science and cell biology, with special emphasis in 3D sample
preparation. Boeckeler Instruments, Inc. is an Equal Opportunity/Affirmative Action Employer.

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From: microscopy.listserver-at-gmail.com
Date: Sat, 26 Sep 2020 06:24:09 -0500
Subject: [Microscopy] Fwd: Re: viaWWW:software script allowing the automatic

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X-from: V.Ray {vray-at-partbeamsystech.com}


Hi Margarita,

Haven't seen it used with SEMs yet, but (unless you find another off-the-shelf plug-and-play
solution) I would suggest you to look at

https://micro-manager.org/

This open-source package ss often used for automating wide area acquisitions with optical
microscopes, has built-in autofocus routine, and integrate-able with ImageJ and Matlab. If remote
control protocol for your SEM is known then it very much could be adapted for your application.

Just thinking out loud - no connection with any of the mentioned SW packages or their manufacturers
here...

Would also suggest to make a summary of the responses you get, as wide area image acquisition is of
interest and information you gather could be beneficial to quite a few people .

Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
www.partbeamsystech.com
www.fibsemproducts.com
www.freudlabs.com

"Only the Paranoid Survive" (A.Grove & SpaceX QA)

On 9/25/2020 5:39 PM, microscopy.listserver-at-gmail.com wrote:
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} Email: sobol-at-img.cas.cz Name: Margarita Sobol
}
} Title-Subject: [Filtered] the software script allowing the automatic focusing of the samples during
} the EM high-throughput acquisition
}
} Message: I use Jeol 1400 Flash EM, which has a montage system using the stage drive to shift the
} field of view (Limitless Panorama). This system enables a montage panorama image to be captured over
} a wide area. Actually, I acquire the grid squares one by one and I always focus each grid square
} separately. Also I check if the focus is OK for each corner and the center of the grid square.
} Usually, it is so when the acquisition starts, but after the first hour (LLP lasts 6-8 hours for the
} grid square at x30K) the image becomes to be out of focus and I need to pause the capturing and
} adjust the focus manually. This means that the overnight LLP is often useless for the analysis.
} I need the software script which allows the automatic focusing of the samples during the EM
} high-throughput acquisition
} Thanks a lot in advance!
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From: Reinhard.Rachel-at-biologie.uni-regensburg.de
Date: Sun, 27 Sep 2020 07:24:57 -0500
Subject: [Microscopy] software script incl automatic focusing

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Hi Margarita,

three comments.

1. you work at a Mag of 30k? most likely, on this TEM, you have a bottom-mount camera installed, haven't you? the last time, when I tested this TEM, it had a bottom-mount camera installed. - IF SO: the pixel size on the detector might be in the range of 0.3 to 0.5 nm. Assuming that you work on an ultrathin section, this pixel size is too small (IMO), and you can easily go to 10k or 15k and save a lot of time for collecting the data. Already at Mag 15k, you have 1/4 of the data and you are about ready in 1/4 or 1/3 of the time (dep. on the overhead during data collection). But you do not loose any relevant information, on sections (I guess).

2. For a full grid montage at this MAG, I can imagine that NO grid is flat enough that you do not have to readjust the focus, from time to time. I see your problem, easily. - I wonder if JEOL can provide a solution for this problem? if they offer something like LLP, then regular focussing is mandatory. Also JEOL should be aware of this - you may contact the application specialists, who are in charge for you.

3. The 1400 Flash can easily be run using SerialEM, with almost most modern cameras (look at the webpage of SerialEM , and ask Guenter Resch - Nexperion, Wien - and David Mastronarde). With SerialEM, it is well possible to perform what you want to do: to have a script collecting the data of a full grid and to do regular Autofocus. - you need SerialEM - then install it or have it installed. And then stage calibration, calibration of image shift and beam shift, and autofocus calibration. Then the wonderful SerialEM Navigator - built in - and a script.

kind regards,
Reinhard

--
Prof. Dr. Reinhard Rachel
University of Regensburg
Centre for EM / Anatomy
Faculty of Biology & Preclin. Med.
Universitaetsstrasse 31
D-93053 Regensburg - Germany
tel +49 941 943 -2837, -1720
mail reinhard.rachel-at-biologie.uni-regensburg.de
office: VKL 3.1.29
member of the IFSM board

Next microscopy conferences:
- next European EM conf.: EMC2024 in Kopenhagen, August 2024
- MC2021 in Vienna, 22-26 Aug 2021(D-A-CH + MCM conference)
- IMC20 in Busan, South Korea: Sept 25-30, 2022
- next Microbiol. conferences: VAAM March 2021 Dsseldorf





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From: microscopy.listserver-at-gmail.com
Date: Mon, 28 Sep 2020 07:29:42 -0500
Subject: [Microscopy] viaWWW: SOS need PDF file 00-008-0342

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Email: philippe.buffat-at-epfl.ch Name: P.Buffat

Title-Subject: [Filtered] SOS PDF file 00-008-0342

Message: Would somebody be able and so kind to send me a copy of the ICCD (ex-JCPDS) files PDF
00-008-0342 (tetragonal HfO2).

This information is needed to confirm my crystal phase identification made with the ICSD7146
structure, answer to a reviewer who disagree with it and get the final acceptance of a manuscript
before Sep 30.
Our university ICSD database looks out of order for a week (Corona-virus?). This file is often used
in literature for the HfO2 tetragonal phase, but the Bravais parameters, space group and author
reference are never explicitly given.
Thanks for your help
Philippe Buffat

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From: vray-at-partbeamsystech.com
Date: Mon, 28 Sep 2020 17:21:43 -0500
Subject: [Microscopy] Paper from 1983 proceedings of SEM Inc. conference

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear Listers,

I am trying to locate a paper which seems to have been published in
proceedings of Scanning Electron Microscopy conference held back in 1983
in Chicago, IL by SEM Inc.:

"Measurement of elastically reflected electrons (E { 2.5 keV) for imaging
of surfaces in a simple ultra high vacuum scanning electron microscope"
R Schmid, KH Gaukler, H Seiler - Scanning Electron Microscopy, 1983

Neither the paper nor proceedings aren't available through library
system (at least within my ability to navigate it), so hoping that maybe
someone else with persistent habit of reading (and keeping) paper books
may have a copy...

Thank you very much beforehand,
Valery
--
Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
www.partbeamsystech.com
www.fibsemproducts.com
www.freudlabs.com

"Only the Paranoid Survive" (A.Grove & SpaceX QA)

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From: benada-at-biomed.cas.cz
Date: Wed, 7 Oct 2020 03:20:15 -0500
Subject: [Microscopy] EDS - copper grid?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

I have a question about the EDX line profile measurements for the INCA software users, I hope there are some here :)

We use INCA software and Oxford EDX detector for EDX measurements with our JEOL 2100F TEM. After taking line profile measurements, we have 200 points in the line, for each point one spectrum. How do you extract the data? I only found extraction for each point-spectrum by hand, which is really weird and long for 200 points.


Thank you very much in advance!

With my best wishes,
Vita Solovyeva



Dr. Vita Solovyeva
University of Oldenburg
Fak V- Institute of Physics
Carl von Ossietzky-Strae 9-11
26129 Oldenburg
Tel. +49-4417983547
Vita.solovyeva-at-uni-oldenburg.de
https://uol.de/fk5/elektronenmikroskopie



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10, 36 -- Subject: TEM: EDX, INCA software, line profile measurements
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From pyledary522s-at-gmail.com Wed Sep 30 20:36:52 2020
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Message-ID: {E5068D90.F32246C9-at-gmail.com}

Dear all,
Please, could anybody give me some hints on the following problem? We
have a coppers grid with ultrathin sections of culture cells. The cells
were cultured in the medium containing CuO nanoparticles (NPs). Our
colleagues want to prove by EDS that the electron opaque clusters found
in the sections have copper. The specimen is unique.

We were aware of the artifact of the additional copper signal induced
by the copper grid. Nevertheless, we have tried to record EDS spectra
from those clusters and nearby within the ultrathin section. When we
measured the spectra in the ultrathin section, we got the artificial
Cu-K peak caused by a copper grid and no Cu-L peak. However, we
obtained a pretty nice Cu-L peak and Cu-K peak when we measured the
spectra from NPs clusters.

EDS spectra acquisition conditions: JEOL F200 (200 kV), 30 ls, all
other settings were the standard setting of the microscope - we were
unallowed to change any settings.

I am thanking you in advance for any comments.


Best regards,
Oldrich

--
Oldřich Benada
Institute of Microbiology, Czech Acad. Sci.
Laboratory of Molecular Structure Characterization
Electron Microscopy Group
Vídeňská 1083
142 20 Prague 4
Czech Republic


Upozorneni:
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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Oct 2020 13:57:11 -0500
Subject: [Microscopy] viaWWW: Research Fellow positions available at CMCAUWA

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello,

I am trying to rebuild the computer for a SEM FEI Quanta 200 on a HP XW
4600.

I have first restored a FEI windows XP image and then installed
Quanta_2.42_i1401. I followed all the installation instructions from the
pdf which came with this DVD kit. Motion controller didn't work, DSPB
pci board does not load the dspb.sys driver correctly.

Then I restored a FEI windows 2k image on the HP XW 4600 and installed
Quanta_2.42_i1401. Thsi time motion seams to be working, but I have the
same error related to DSPB driver.

Anybody had this issue before? Do I need another dspb.sys? Is there any
other file needed for the DSPB board? My board is PM DIG SCAN & PAT BRD
DSPB 4022 192 91222

Regards,

--
Cătălin Marinescu, Engr., PhD.
+40722233858

Asociația Independent Research
Str. Timișului nr 58, sect.1
București - 012416
România
www.independent-research.ro

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Email: whiteto-at-missouri.edu Name: Tommi White

Title-Subject: [Filtered] Faculty position in Biochemistry at University of Missouri

Message: Dear Microscopy List-serve colleagues,
University of Missouri is recruiting for a faculty member (all levels!) in the Department of
Biochemistry. Applications are due November 16th, 2020.
https://biochem.missouri.edu/open-positions/
CryoEM is an area we wish to strengthen, including in situ cryoET. The Universitys Electron
Microscopy Core will be installing the following new microscopes in the $260M NextGen Precision
Health Institute: Titan Krios G4 CryoTEM with Bioquantum K3, Aquilos 2, Leica Thunder CryoCLEM and
VolumeScope. Additional instruments to be added include Helios Hydra UX PFIB with SIMS-TOF and
Spectra Aberration-Corrected TEM with Continuum K3. These additions are part of a recent
partnership with Thermo Fisher Scientific to create a Center of Excellence in Electron Microscopy
with the University of Missouri System focused on 3 areas: instrumentation innovation,
research/collaboration between basic/clinical sciences, and workforce development with STEM training
and education. This is a great opportunity to get on the ground floor and help us build something
amazing.
Please feel free to reach out to me with any questions! We look forward to your applications and
would appreciate if you could let interested colleagues know too!
Tommi A. White, Ph.D.
Director, Electron Microscopy Core
Assistant Research Professor, Biochemistry
University of Missouri
Mail: W117 Veterinary Medicine Bldg
1520 E Rollins St., Columbia, MO 65211
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From tammyhoward072fu-at-gmail.com Mon Oct 12 14:26:32 2020
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X-from: Peta Clode {peta.clode-at-uwa.edu.au}


Were hiring! CMCA-at-UWA are currently advertising two Research Fellow (Level B) positions, which are
available for immediate start. If you have the right skills and like sun, surf, short commutes, and
microscopes apply now!

1. *CryoTEM specialist*

Exciting opportunity for a passionate researcher with cryoTEM experience to be a key player in the
establishment of the first cryoTEM facility in Western Australia and to work in a multi-disciplinary
research environment developing and supporting imaging and diffraction applications. Fixed term
appointment until 30 June 2023 with an immediate start. Salary range: Level B $100,374 p.a. -
$118,776 p.a. plus superannuation

https://external.jobs.uwa.edu.au/en/job/504994/research-fellow-cryotem

2. *Multi-modal specialist*

Applications are open to researchers with skills in microscopy techniques and/or associated data
processing methods who are looking for an exciting opportunity to develop novel multi-modal
applications in a dynamic, interdisciplinary team environment. Fixed term appointment until 30 June
2023 with an immediate start. Salary range: Level B $100,374 p.a. - $118,776 p.a. plus superannuation

https://external.jobs.uwa.edu.au/en/job/504993/research-fellow-multimodal-microscopy

Cheers,

Peta

**

*Associate Professor Peta L. Clode*

*Platform Leader: Biosciences Electron Microscopy*

Centre for Microscopy, Characterisation and Analysis

UWA School of Biological Sciences

UWA Oceans Institute

M010,Perth WA 6009 Australia

+61 8 6488 8098 peta.clode-at-uwa.edu.au






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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Oct 2020 15:39:52 -0500
Subject: [Microscopy] EDS on Glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Frank Karl {frank_karl-at-ardl.com}

Hello everyone,
I've been asked to help out by distinguishing between type E, R and D glass by elemental
composition from EDS analysis. I thought I have references to the elemental composition from past
standards, but they apparently have been place somewhere so safe I can't find them.
I found several references but the most useful combined Ca and Mg as a single measurement.

Unfortunately we don't have standards, otherwise I'd simple re-run them.

Any help would be useful.

Thanks!




Stay calm...Be brave....watch for signs

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305


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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Oct 2020 17:32:13 -0500
Subject: [Microscopy] Fwd: Re: [EXTERNAL] EDS on Glass

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X-from: Smart, Thomas Philip {tsmart-at-eastman.com}


Hello Frank,
With a lack of standards to reference, the Saint Gobain website
https://glassproperties.com/glasses/E_R_and_D_glass_properties.pdf shows you should be able to tell
the difference between the three types of glass by general elemental composition... notably the Al,
Ca, and B levels in each.
Hope this helps you.

Thomas

Thomas Smart
Microscopy Scientist
Eastman Chemical Company

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} X-from: Frank Karl {frank_karl-at-ardl.com}
}
} Hello everyone,
} I've been asked to help out by distinguishing between type  E, R and D glass by elemental
} composition from EDS analysis.  I thought I have references to the elemental composition from past
} standards, but they apparently have been place somewhere so safe I can't find them.
} I found several references but the most useful combined Ca and Mg as a single measurement.
}
} Unfortunately we don't have standards, otherwise I'd simple re-run them.
}
} Any help would be useful.
}
} Thanks!
}
}
}
}
} Stay calm...Be brave....watch for signs
}
} Frank Karl
} Microscopist
} Akron Rubber Development Laboratory
} 2887 Gilchrist Road
} Akron, Ohio  44305
}
}
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From: microscopy.listserver-at-gmail.com
Date: Tue, 13 Oct 2020 17:32:39 -0500
Subject: [Microscopy] Fwd: Re: EDS on Glass

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Jim Quinn {james.quinn-at-stonybrook.edu}


I doubt this info helps:

https://glassproperties.com/glasses/E_R_and_D_glass_properties.pdf

https://glassproperties.com/glasses/

Please let me know if you get better responses.

- Jim




On Tue, Oct 13, 2020 at 4:51 PM {microscopy.listserver-at-gmail.com
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X-from: Frank Karl {frank_karl-at-ardl.com {mailto:frank_karl-at-ardl.com} }

Hello everyone,
I've been asked to help out by distinguishing between type  E, R and D glass by elemental
composition from EDS analysis.  I thought I have references to the elemental composition from past
standards, but they apparently have been place somewhere so safe I can't find them.
I found several references but the most useful combined Ca and Mg as a single measurement.

Unfortunately we don't have standards, otherwise I'd simple re-run them.

Any help would be useful.

Thanks!




Stay calm...Be brave....watch for signs

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio  44305


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From: microscopy.listserver-at-gmail.com
Date: Thu, 15 Oct 2020 06:39:29 -0500
Subject: [Microscopy] viaWWW:Job opening for a PhD position in cryo-EM and correlative

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X-from: lieberw-at-mpip-mainz.mpg.de

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Email: lieberw-at-mpip-mainz.mpg.de Name: Ingo Lieberwirth

Title-Subject: [Filtered] Job opening for a PhD position in cryo-EM and correlative microscopy

Message: The MPI for Polymer Research is currently advertising a PhD position in correlative
microscopy of colloids. This position offers the great opportunity to work with a unique cryo-TEM.
Currently we are installing a Titan Krios G4 with a K3 DED Continuum EELS. The research project
includes the development of novel strategies to correlate the information from superresolution light
microscopy with the high resolution information from the EM.
Detailed information can be found here:
www.mpip-mainz.mpg.de/500074/PhD_position_in_correlative_microscopy_of_colloids_2?c=102396

or just contact me by e-mail (lieberw-at-mpip-mainz.mpg.de).

Best,
Ingo

__________________________________________________
Dr. Ingo Lieberwirth
Head of EM core facility
MPI for Polymer Research
Mainz, Germany


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From: microscopy.listserver-at-gmail.com
Date: Thu, 15 Oct 2020 06:42:06 -0500
Subject: [Microscopy] Fwd: Thin dense slice burning hole in carbon film during EBSD

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X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz}

Hi

We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB).
The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we
load a FIB produced slice mounted on a carbon film coated copper grid, the slice disappears within
a short time of the beam going onto it.
The hole remaining in the carbon film is the same shape as the original slice so I suspect that the
slice is heating up and burning a hole in the film. The remainder of the film remains intact.

Any thoughts on how to mitigate this?

Thanks

Allan


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From: microscopy.listserver-at-gmail.com
Date: Thu, 15 Oct 2020 06:43:39 -0500
Subject: [Microscopy] EDS on Glass

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X-from: John Yorston {ls3foxdelta-at-verizon.net}



https://glassproperties.com/glasses/E_R_and_D_glass_properties.pdf


-----Original Message-----
X-from: microscopy.listserver-at-gmail.com
To: ls3foxdelta-at-verizon.net
Sent: Tue, Oct 13, 2020 4:47 pm

X-from: Frank Karl {frank_karl-at-ardl.com {mailto:frank_karl-at-ardl.com} }

Hello everyone,
I've been asked to help out by distinguishing between type  E, R and D glass by elemental
composition from EDS analysis.  I thought I have references to the elemental composition from past
standards, but they apparently have been place somewhere so safe I can't find them.
I found several references but the most useful combined Ca and Mg as a single measurement.

Unfortunately we don't have standards, otherwise I'd simple re-run them.

Any help would be useful.

Thanks!




Stay calm...Be brave....watch for signs

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio  44305


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From: crwinkler-at-ncsu.edu
Date: Thu, 15 Oct 2020 10:13:34 -0500
Subject: [Microscopy] Re: Fwd: Thin dense slice burning hole in carbon film

Contents Retrieved from Microscopy Listserver Archives
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Hello Allan,

It could be sample heating, sample charging, the carbon sputtering
away, or some mechanical vibration of the sample is the culprit of
your film failures. Carbon films are resilient at lower beam currents
but can quickly be sputtered away during STEM experiments and when
higher beam currents are used in TEM mode, so it's possible the
aggressive beam current settings needed for good EBSD are sputtering
the carbon away (assuming lamella is electron transparent; if thick,
then the excitation volume will be mostly confined to your sample). If
your sample is an insulator, it may be charging and physically
vibrating during the discharge-charge cycle and punching a hole in the
carbon film. Depending on the thickness of the lamella and how
conductive it is, you could be heating the lamella to higher
temperatures during the EBSD acquisition. Carbon films are generally
fine up to hundreds of celsius, but the local heating of the lamella
may be stressing the carbon film and causing it to rupture. You could
also be puncturing the film slightly during the landing of the lamella
and the electron beam will easily shred the carbon film. Lots of
things to consider, and quite complicated depending on how thick the
lamella is.

Are you using "thick" carbon films or ultrathin? Are you able to
anchor the FIB lamella to one of the metal grid bars using Pt/W
deposition? Could you land the lamella as close to a corner of the
carbon foil? Alternatively, if you're able to change support films SiN
windows are very mechanically robust and unlikely to fail. If you need
to use carbon films, you may try to use a lacey or holey carbon film
and anchor the lamella using Pt/W to a couple of carbon filaments. Not
trivial to accomplish but it's been done before. I suspect you're
trying to do something in situ with the lamella, so your options may
be limited.

Good luck,
Chris


On Thu, Oct 15, 2020 at 8:02 AM {microscopy.listserver-at-gmail.com} wrote:
}
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} X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz}
}
} Hi
}
} We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB).
} The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we
} load a FIB produced slice mounted on a carbon film coated copper grid, the slice disappears within
} a short time of the beam going onto it.
} The hole remaining in the carbon film is the same shape as the original slice so I suspect that the
} slice is heating up and burning a hole in the film. The remainder of the film remains intact.
}
} Any thoughts on how to mitigate this?
}
} Thanks
}
} Allan
}
}
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--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
https://www.aif.ncsu.edu/
Cell: 267-496-0587

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9, 55 -- In-Reply-To: {202010151151.09FBpSqn028781-at-microscopy.com}
9, 55 -- From: Christopher Winkler {crwinkler-at-ncsu.edu}
9, 55 -- Date: Thu, 15 Oct 2020 11:24:32 -0400
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9, 55 -- Subject: Re: [Microscopy] Fwd: Thin dense slice burning hole in carbon film
9, 55 -- during EBSD
9, 55 -- To: Microscopy-at-microscopy.com
9, 55 -- Cc: Christopher Winkler {crwinkler-at-ncsu.edu} , allan.mitchell-at-otago.ac.nz
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==============================End of - Headers==============================




From: vray-at-partbeamsystech.com
Date: Thu, 15 Oct 2020 17:52:10 -0500
Subject: [Microscopy] Re: Fwd: Thin dense slice burning hole in carbon film

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Some details on the nature of the sample could have been helpful, but if
I had to guess:

Try doing FIB deposition on SiN window instead of C grid. Prior to
depositing sample, etc..., coat SiN window with C evaporation and make
sure to provide reliable connection to ground for charge dissipation.

If the "dense slice" isn't conductive it may be blowing up by ESD due to
charging by high electron beam current - if so you could try depositing
couple of nm C on the side facing away from EBSD camera, and lowering
acceleration voltage.

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
www.partbeamsystech.com
www.fibsemproducts.com
www.freudlabs.com

"Only the Paranoid Survive" (A.Grove & SpaceX QA)

On 10/15/2020 7:42 AM, microscopy.listserver-at-gmail.com wrote:
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} X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz}
}
} Hi
}
} We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB).
} The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we
} load a FIB produced slice mounted on a carbon film coated copper grid, the slice disappears within
} a short time of the beam going onto it.
} The hole remaining in the carbon film is the same shape as the original slice so I suspect that the
} slice is heating up and burning a hole in the film. The remainder of the film remains intact.
}
} Any thoughts on how to mitigate this?
}
} Thanks
}
} Allan
}
}
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From: microscopy.listserver-at-gmail.com
Date: Thu, 15 Oct 2020 19:11:53 -0500
Subject: [Microscopy] viaWWW:Looking For: Industry Certifications Suitable for High School

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Title-Subject: [Filtered] Looking For: Industry Certifications Suitable for High School Students

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From: koeck-at-kth.se
Date: Fri, 16 Oct 2020 02:15:37 -0500
Subject: [Microscopy] VB: Magnetic field in TEM

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Hi.

I'm looking for information on the magnetic field produced by the objective lens of a typical 200 kVTEM with a focal length of roughly 3 mm, for examplethe Jeol 2100 with the cryo pole piece.

Also I'd like to know what the field would be if the lens is weakened so much that the focal length is in the order of 2 to 3 cm.

Does anybody have plots or values for (some of) this, or is there some free software to do the calculations with realistic pole piece geometries?

All the best,

Philip



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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Oct 2020 06:46:44 -0500
Subject: [Microscopy] Fwd: Re: Fwd: Thin dense slice burning hole in carbon

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X-from: V.Ray {vray-at-partbeamsystech.com}

Some details on the nature of the sample could have been helpful, but if I had to guess:

Try doing FIB deposition on SiN window instead of C grid. Prior to depositing sample, etc..., coat
SiN window with C evaporation and make sure to provide reliable connection to ground for charge
dissipation.

If the "dense slice" isn't conductive it may be blowing up by ESD due to charging by high electron
beam current - if so you could try depositing couple of nm C on the side facing away from EBSD
camera, and lowering acceleration voltage.

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
www.partbeamsystech.com
www.fibsemproducts.com
www.freudlabs.com

"Only the Paranoid Survive" (A.Grove & SpaceX QA)

On 10/15/2020 7:42 AM, microscopy.listserver-at-gmail.com wrote:
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} X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz}
}
} Hi
}
} We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB).
} The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we
} load a FIB produced slice mounted on a carbon film coated copper grid, the slice disappears within
} a short time of the beam going onto it.
} The hole remaining in the carbon film is the same shape as the original slice so I suspect that the
} slice is heating up and burning a hole in the film. The remainder of the film remains intact.
}
} Any thoughts on how to mitigate this?
}
} Thanks
}
} Allan
}
}
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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Oct 2020 06:47:14 -0500
Subject: [Microscopy] Fwd: Thin dense slice burning hole in carbon film

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X-from: s.walck-at-comcast.net


Is there any chance that your sample is hydrated or is reacting under the
beam to release oxygen? Try another sample material like silicon and see if
it still a problem. If it is, you might have a vacuum problem in your
microscope -water or air leak.

If it is, it's reacting with the film and beam. I would try silicon nitride
films.
-Scott

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, October 15,
2020 7:44 AM
To: s.walck-at-comcast.net

X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz}

Hi

We are trying to do EBSD (in an SEM) on a dense thin slice (produced by
FIB).
The thin slice is mounted on a carbon film, on a copper TEM grid. The
problem is that each time we load a FIB produced slice mounted on a carbon
film coated copper grid, the slice disappears within a short time of the
beam going onto it.
The hole remaining in the carbon film is the same shape as the original
slice so I suspect that the slice is heating up and burning a hole in the
film. The remainder of the film remains intact.

Any thoughts on how to mitigate this?

Thanks

Allan

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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Oct 2020 06:47:43 -0500
Subject: [Microscopy] Fwd: Re: Fwd: Thin dense slice burning hole in carbon

Contents Retrieved from Microscopy Listserver Archives
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X-from: Nick Seaton {seato008-at-umn.edu}



I would suggest using something like a SiN window grid rather than a carbon grid. They should be
much more resistant to heating related problems.



On Thu, Oct 15, 2020 at 6:54 AM {microscopy.listserver-at-gmail.com
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X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz {mailto:allan.mitchell-at-otago.ac.nz} }

Hi

We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB).
The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we
load a FIB produced slice mounted on a carbon film coated copper grid,  the slice disappears within
a short time of the beam going onto it.
The hole remaining in the carbon film is the same shape as the original slice so I suspect that the
slice is heating up and burning a hole in the film.  The remainder of the film remains intact.

Any thoughts on how to mitigate this?

Thanks

Allan


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--

Dr Nicholas Seaton

SEM, FIB & LM Specialist
Department Safety Officer
Characterization Facility

College of Science and Engineering

University of Minnesota

12 Shepherd Labs

100 Union Street SE

Minneapolis

MN, 55455

email: seato008-at-umn.edu {mailto:seato008-at-umn.edu}

phone: 612-626-5314


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From: microscopy.listserver-at-gmail.com
Date: Fri, 16 Oct 2020 06:48:39 -0500
Subject: [Microscopy] Fwd: Re: Fwd: Thin dense slice burning hole in carbon

Contents Retrieved from Microscopy Listserver Archives
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X-from: Bil Schneider {wfschneider-at-wisc.edu}

Allan,
Have you considered coating the whole sample? We use 1nm of Iridium for EBSD which works for most
samples including fragile biological specimens. I don’t know how thick your “dense thin slice”is?

Bil Schneider SEM Lab Manager
UW Madison Geosciences 608-333-7874



On Oct 15, 2020, at 6:57 AM, microscopy.listserver-at-gmail.com wrote:




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X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz}

Hi

We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB).
The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we
load a FIB produced slice mounted on a carbon film coated copper grid, the slice disappears within
a short time of the beam going onto it.
The hole remaining in the carbon film is the same shape as the original slice so I suspect that the
slice is heating up and burning a hole in the film. The remainder of the film remains intact.

Any thoughts on how to mitigate this?

Thanks

Allan


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From: microscopy.listserver-at-gmail.com
Date: Sat, 17 Oct 2020 07:26:31 -0500
Subject: [Microscopy] viaWWW: Using Methanol instead of Water in Single Particle Cryo-EM.

Contents Retrieved from Microscopy Listserver Archives
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Dear Mrs.,

We were able to recover a Leica Cambridge S360 scanning electron
microscope that worked for some time.
For some reason the license diskette has been damaged.
Would any of you have a copy of the SEM S360 license diskette V03.03
to send, borrow or sell?

Thanks for listening,
Antonio.

==============================Original Headers==============================
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From lambertkeng249zebo-at-gmail.com Sat Oct 17 02:29:55 2020
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Email: sayitugurlu-at-gmail.com Name: Sayit Ugurlu

Title-Subject: [Filtered] Using Methanol instead of Water in Single Particle Cryo-EM.
Message: Hi,

Is there any one who try to use methanol in Cryo-EM instead of water? I just wonder because the
vitrified water has 1000 kg/m3 density and the vitrified methanol has approximate 800 kg/m3 density.
So if someone can put his/her sample inside methanol and can vitrified it, he/she can get a better
contrast. Surroundings of the protein would be less dense So this can give better contrast to the
protein.
Of Course, there would be some issue using methanol. I am just asking this because of my curiosity.
Best wishes,
Sayit Ugurlu

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From: microscopy.listserver-at-gmail.com
Date: Sat, 17 Oct 2020 07:27:15 -0500
Subject: [Microscopy] viaWWW: Research Support Specialist position at the Electron

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Email: amaliapasolli-at-gmail.com Name: Hilda Amalia Pasolli

Title-Subject: [Filtered] Research Support Specialist position at the Electron Microscopy Resource
Center

Message: Hi, there is an opening in my lab for a:

Research Support Specialist
Electron Microscopy Resource Center
The Rockefeller University
1230 York Avenue,
New York, NY 10065


Rockefeller University is the worlds leading biomedical research University. Our groundbreaking
discoveries in basic and clinical research are transforming medicine. We share a singular commitment
to advancing science for the benefit of humanity. Our collaborative culture drives each of us to
achieve a higher level, fueling the breakthroughs for which we are known.
We seek a meticulous and detail-oriented Research Specialist to join our Electron Microscopy
Resource Center (EMRC), who enjoys working in an intellectually rich and multi-disciplinary environment.
Center Description

The Electron Microscopy Resource Center (EMRC) provides state-of-the-art electron microscopy support
for analysis of a wide variety of biological samples, including viruses, bacteria, animal tissues as
well as cultured cells and isolated cellular components for ultrastructural analyses or
immuno-electron microscopy. The EMRC is equipped with two transmission electron microscopes, a
conventional scanning electron microscope, and a high-pressure freezing and a freeze-substitution
unit. (http://www.rockefeller.edu/emrc/)

Job Description
Reporting to the Director of the EMRC, the Research Support Specialist will perform specimen
preparation, including fixation, resin embedding, negative staining, ultrathin sectioning, and
immunolabeling, operate the microscopes and associated equipment, train users, and consults with
scientists on the design of experiments, data processing/analysis, interpretation of results, and
informs users about the latest methodologies through familiarity with relevant literature. The
Research Support Specialists will participate in all of EMRC daily operations, including
maintenance, upkeep of the electron microscopes, associated equipment, and laboratory space,
ordering supplies, and administrative tasks, including center billing. Qualifications

M.S. or Ph.D. degree or equivalent background in biology, bioengineering or a related field
A minimum of five years of hands-on experience in electron microscopy, including biological sample
preparation, ultramicrotomy, immuno-electron microscopy, image analysis, and interpretation
Ability to produce high-quality EM samples and images
Affinity for tasks requiring fine motor skills
Strong communication skills and the ability to work collaboratively in a team as well as
independently on a wide variety of research projects
Ability to manage time efficiently, complete tasks on time, and to adapt to unscheduled requests
Detail-oriented, focused, and highly motivated
A strong background in computation and image acquisition/analysis programs (SerialEM, Image J)
would be a plus


How to apply
We offer a competitive salary, comprehensive benefits, and a collaborative working environment.
Please visit the URL below and apply to the job code IRC25045. Please make sure to upload your
resume and a cover letter:
http://www2.rockefeller.edu/hr/jobs.php?url=https://recruit.rockefeller.edu/OA_HTML/OA.jsp?OAFunc=IRC_VIS_VAC_DISPLAY%26p_svid=25045%26p_spid=986026%26p_site_id=4361
Rockefeller University is an Equal Opportunity Employer - Minorities/Women/Disabled/Veterans

Hilda Amalia Pasolli, Ph.D.
Director and Research Associate Professor
Electron Microscopy Resource Center
RRB 120F
The Rockefeller University
1230 York Avenue,
Box 230
New York, NY 10065



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From: tsmart-at-eastman.com
Date: Sat, 17 Oct 2020 11:27:59 -0500
Subject: [EXTERNAL] [Microscopy] viaWWW: Using Methanol instead of Water in Single Particle Cryo-EM.

Contents Retrieved from Microscopy Listserver Archives
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Good morning Sayit,

I previously worked with methanol - it's possible...
We were looking at block copolymer micelles in solution in methanol. Two points made it tricky: 1) The methanol evaporates very quickly compared to water to sample volumes and blotting times need to be experimented with for you specific sample, and 2) the boiling point of methanol is obviously much lower than water so you have to work very fast/low dose in the TEM so that your sample doesn't disappear in front of your eyes! Reference below....

Macromolecules 2011, 44, 24, 9574-9585 https://pubs.acs.org/doi/abs/10.1021/ma2020936

Synthesis and Characterization of Amphiphilic Cyclic Diblock Copolypeptoids from N-Heterocyclic Carbene-Mediated Zwitterionic Polymerization of N-Substituted N-Carboxyanhydride

Thomas

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Email: sayitugurlu-at-gmail.com Name: Sayit Ugurlu

Title-Subject: [Filtered] Using Methanol instead of Water in Single Particle Cryo-EM.
Message: Hi,

Is there any one who try to use methanol in Cryo-EM instead of water? I just wonder because the
vitrified water has 1000 kg/m3 density and the vitrified methanol has approximate 800 kg/m3 density.
So if someone can put his/her sample inside methanol and can vitrified it, he/she can get a better
contrast. Surroundings of the protein would be less dense So this can give better contrast to the
protein.
Of Course, there would be some issue using methanol. I am just asking this because of my curiosity.
Best wishes,
Sayit Ugurlu

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18, 80 -- Subject: RE: [EXTERNAL] [Microscopy] viaWWW: Using Methanol instead of Water
18, 80 -- in Single Particle Cryo-EM.
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From: koeck-at-kth.se
Date: Sun, 18 Oct 2020 01:59:43 -0500
Subject: [EXTERNAL] [Microscopy] viaWWW: Using Methanol instead of Water in Single Particle Cryo-EM.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi.

The important quantity for phase contrast TEM is the electrostatic potential, not the density.
You'd have to check how much the mean inner potential of water and methanol differ to see how much contrast you gain.
Then there's also the biological question. Is the protein functional in methanol and does it keep its native conformation?

It's certainly an interesting idea, in any case.

Philip
________________________________________
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Skickat: den 17 oktober 2020 18:35
Till: Philip Kck
mne: [Microscopy] RE: [EXTERNAL] viaWWW: Using Methanol instead of Water

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Good morning Sayit,

I previously worked with methanol - it's possible...
We were looking at block copolymer micelles in solution in methanol. Two points made it tricky: 1) The methanol evaporates very quickly compared to water to sample volumes and blotting times need to be experimented with for you specific sample, and 2) the boiling point of methanol is obviously much lower than water so you have to work very fast/low dose in the TEM so that your sample doesn't disappear in front of your eyes! Reference below....

Macromolecules 2011, 44, 24, 9574-9585 https://pubs.acs.org/doi/abs/10.1021/ma2020936

Synthesis and Characterization of Amphiphilic Cyclic Diblock Copolypeptoids from N-Heterocyclic Carbene-Mediated Zwitterionic Polymerization of N-Substituted N-Carboxyanhydride

Thomas

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Email: sayitugurlu-at-gmail.com Name: Sayit Ugurlu

Title-Subject: [Filtered] Using Methanol instead of Water in Single Particle Cryo-EM.
Message: Hi,

Is there any one who try to use methanol in Cryo-EM instead of water? I just wonder because the
vitrified water has 1000 kg/m3 density and the vitrified methanol has approximate 800 kg/m3 density.
So if someone can put his/her sample inside methanol and can vitrified it, he/she can get a better
contrast. Surroundings of the protein would be less dense So this can give better contrast to the
protein.
Of Course, there would be some issue using methanol. I am just asking this because of my curiosity.
Best wishes,
Sayit Ugurlu

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18, 80 -- Subject: RE: [EXTERNAL] [Microscopy] viaWWW: Using Methanol instead of Water
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From: nizets2-at-yahoo.com
Date: Mon, 19 Oct 2020 09:41:38 -0500
Subject: [EXTERNAL] [Microscopy] viaWWW: Using Methanol instead of Water in Single Particle Cryo-EM.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi!


Just my cents:
It is an interesting question but as always with science, the first question to ask is:
Does it make any sense?


What I mean is, the main reason Cryo-EM was developed is to keep the system as native as possible and thus avoid the artifacts coming from preparing the material in artificial conditions. Since most biological systems swim in some sort of watery soup, water seems to be the optimal medium for cryo-EM, not because the properties of water are optimal for observation in TEM but because it is the most meaningful medium.


Regards,
Stephane






On Sunday, October 18, 2020, 09:15:52 AM GMT+2, koeck-at-kth.se {koeck-at-kth.se} wrote:








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Hi.

The important quantity for phase contrast TEM is the electrostatic potential, not the density.
You'd have to check how much the mean inner potential of water and methanol differ to see how much contrast you gain.
Then there's also the biological question. Is the protein functional in methanol and does it keep its native conformation?

It's certainly an interesting idea, in any case.

Philip
________________________________________
Från: tsmart-at-eastman.com {tsmart-at-eastman.com}
Skickat: den 17 oktober 2020 18:35
Till: Philip Köck
Ämne: [Microscopy] RE: [EXTERNAL] viaWWW: Using Methanol instead of Water

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Good morning Sayit,

I previously worked with methanol - it's possible...
We were looking at block copolymer micelles in solution in methanol.  Two points made it tricky: 1) The methanol evaporates very quickly compared to water to sample volumes and blotting times need to be experimented with for you specific sample, and 2) the boiling point of methanol is obviously much lower than water so you have to work very fast/low dose in the TEM so that your sample doesn't disappear in front of your eyes!  Reference below....

Macromolecules 2011, 44, 24, 9574-9585  https://pubs.acs.org/doi/abs/10.1021/ma2020936

Synthesis and Characterization of Amphiphilic Cyclic Diblock Copolypeptoids from N-Heterocyclic Carbene-Mediated Zwitterionic Polymerization of N-Substituted N-Carboxyanhydride

Thomas

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Email: sayitugurlu-at-gmail.com Name: Sayit Ugurlu

Title-Subject: [Filtered] Using Methanol instead of Water in Single Particle Cryo-EM.
Message: Hi,

Is there any one who try to use methanol in Cryo-EM instead of water? I just wonder because the
vitrified water has 1000 kg/m3 density and the vitrified methanol has approximate 800 kg/m3 density.
So if someone can put his/her sample inside methanol and can vitrified it, he/she can get a better
contrast. Surroundings of the protein would be less dense So this can give better contrast to the
protein.
Of Course, there would be some issue using methanol. I am just asking this because of my curiosity.
Best wishes,
Sayit Ugurlu

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From: stefano-at-soquelec.com
Date: Mon, 19 Oct 2020 09:54:48 -0500
Subject: [EXTERNAL] [Microscopy] viaWWW: Using Methanol instead of Water in Single Particle Cryo-EM.

Contents Retrieved from Microscopy Listserver Archives
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Dear Stephane,

Thank you for your input. It is a very good question (does it make sense?) but I think it does. After all, we use cryo because we cannot observe those systems in their native state. Adding an extra step (methanol) may bring us further away from native conditions but may also enable us to observe features that may not be visible in water. There is always the caveat that those features could be caused by the methanol.

Regards,
Stefano


X-from: nizets2-at-yahoo.com {nizets2-at-yahoo.com}
Sent: Monday, October 19, 2020 07:44 AM
To: Stefano Rubino {stefano-at-soquelec.com}

Hi!


Just my cents:
It is an interesting question but as always with science, the first question to ask is:
Does it make any sense?


What I mean is, the main reason Cryo-EM was developed is to keep the system as native as possible and thus avoid the artifacts coming from preparing the material in artificial conditions. Since most biological systems swim in some sort of watery soup, water seems to be the optimal medium for cryo-EM, not because the properties of water are optimal for observation in TEM but because it is the most meaningful medium.


Regards,
Stephane






On Sunday, October 18, 2020, 09:15:52 AM GMT+2, koeck-at-kth.se {koeck-at-kth.se} wrote:








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Hi.

The important quantity for phase contrast TEM is the electrostatic potential, not the density.
You'd have to check how much the mean inner potential of water and methanol differ to see how much contrast you gain.
Then there's also the biological question. Is the protein functional in methanol and does it keep its native conformation?

It's certainly an interesting idea, in any case.

Philip
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Skickat: den 17 oktober 2020 18:35
Till: Philip Kck
mne: [Microscopy] RE: [EXTERNAL] viaWWW: Using Methanol instead of Water

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Good morning Sayit,

I previously worked with methanol - it's possible...
We were looking at block copolymer micelles in solution in methanol. Two points made it tricky: 1) The methanol evaporates very quickly compared to water to sample volumes and blotting times need to be experimented with for you specific sample, and 2) the boiling point of methanol is obviously much lower than water so you have to work very fast/low dose in the TEM so that your sample doesn't disappear in front of your eyes! Reference below....

Macromolecules 2011, 44, 24, 9574-9585 https://pubs.acs.org/doi/abs/10.1021/ma2020936

Synthesis and Characterization of Amphiphilic Cyclic Diblock Copolypeptoids from N-Heterocyclic Carbene-Mediated Zwitterionic Polymerization of N-Substituted N-Carboxyanhydride

Thomas

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Email: sayitugurlu-at-gmail.com Name: Sayit Ugurlu

Title-Subject: [Filtered] Using Methanol instead of Water in Single Particle Cryo-EM.
Message: Hi,

Is there any one who try to use methanol in Cryo-EM instead of water? I just wonder because the
vitrified water has 1000 kg/m3 density and the vitrified methanol has approximate 800 kg/m3 density.
So if someone can put his/her sample inside methanol and can vitrified it, he/she can get a better
contrast. Surroundings of the protein would be less dense So this can give better contrast to the
protein.
Of Course, there would be some issue using methanol. I am just asking this because of my curiosity.
Best wishes,
Sayit Ugurlu

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18, 80 -- Subject: RE: [EXTERNAL] [Microscopy] viaWWW: Using Methanol instead of Water
18, 80 -- in Single Particle Cryo-EM.
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18, 80 -- in Single Particle Cryo-EM.
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25, 51 -- Subject: SV: [Microscopy] RE: [EXTERNAL] viaWWW: Using Methanol instead of
25, 51 -- Water
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25, 51 -- Water
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48, 39 -- Date: Mon, 19 Oct 2020 14:52:59 +0000 (UTC)
48, 39 -- From: Stephane Nizet {nizets2-at-yahoo.com}
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48, 39 -- Subject: Re: [Microscopy] SV: RE: [EXTERNAL] viaWWW: Using Methanol instead
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From: nessonm-at-oregonstate.edu
Date: Mon, 19 Oct 2020 17:25:24 -0500
Subject: [Microscopy] RCA EMU-2B vs EMU-2D

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Are there any very old-timers still around who can provide information about the differences between an RCA EMU-2B and an EMU-2D.
OSU’s microscope that was donated to a local museum has always been described as an EMU-2B in the EM facility history; but I note that the manufacturer’s label says EMU-2D (Serial No. 775, MI-12957-E).

I seem to remember an seeing an article in Microscopy Today that dealt with the early history of the EMU series, but I haven’t found it yet.

Thanks for your memories!


Michael Nesson, Ph.D.
ALS 2011
Dept Biochem/Biophys
Oregon State University
Corvallis OR



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From: microscopy.listserver-at-gmail.com
Date: Tue, 20 Oct 2020 15:33:14 -0500
Subject: [Microscopy] viaWWW: Looking for a used or demo EDS

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all!

I wondered if any of you already tried to detect viral particles in urine using TEM or SEM?
Clearly one has to isolate somehow the particles from the soup. But perhaps I don't have to reinvent the wheel?
I found many papers using different kinds of chromatography but before I try all of them, maybe I can rely on personal experience from my fellows here? 

Best regards,
Stephane


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Email: amini-at-rowan.edu Name: Shahram Amini

Title-Subject: [Filtered] Looking for a used or demo EDS

Message: Hello colleagues,
We are in the process of purchasing a used ZEISS Supra 35 Variable Pressure FESEM but it doesn't
have EDS capability on it. We are interested in buying a demo or used EDS for our new microscope. If
you have any leads please kindly let me know.
Thanks a lot.
Regards,
Shahram
samini-at-pulsetechnologies.com
amini-at-rowan.edu
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From: jzheng-at-calit2.uci.edu
Date: Thu, 22 Oct 2020 14:23:20 -0500
Subject: [Microscopy] On-line TEM workshop on November 5-7, 2020

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues
We would like to invite you to participate in virtual school "AI for atoms: How to machine learn STEM" to be held at ORNL, December 7-10, 2020 organized by Maxim Ziatdinov, Rama Vasudevan, Debangshu Mukherjee, and Sergei V. Kalinin. The school will feature the combination of invited and contributed talks and hands-on tutorials for AtomAI, GPim, and PyCroscopy packages. If interested, please register at the https://www.surveymonkey.com/r/257WD3B and contact Sergei Kalinin (sergei2-at-ornl.gov) to submit the abstract by November 1. The first call for the school is provided below, and looking forward to (virtually) meeting you at Oak Ridge!
Sergei V. Kalinin


First call:
Machine learning (ML) has emerged as a powerful tool for data and image analysis and as an enabling component of autonomous systems in areas ranging from biological and medical imaging to self-driving cars. This rapid growth in ML applications poses the question as to which of these methods can be applied in electron microscopy, and perhaps more importantly, what insights into the physics and chemistry of real materials can they yield. This virtual school on AI for atoms: how to machine learn STEM, to be held December 7-10, will combine invited and contributed presentations at the forefront of ML applications in Scanning Transmission Electron Microscopy (STEM), Electron Energy Loss Spectroscopy (EELS), and 4D STEM, as well as for physics and chemistry extraction from STEM data sets. It will further feature tutorials on recent developments in ML analysis of mesoscopic and atomically resolved images and spectroscopy in STEM, including classical graph analysis of STEM data, deep convolutional neural networks (DCNNs) for feature identifications, symmetry-invariant (variational) autoencoders ((V)AE), and Gaussian Processes based super-resolution imaging and image reconstruction. The tutorials will be followed by the hands-on tutorial sessions introducing the attendees to the AtomAI (https://github.com/pycroscopy/atomai), GPim (https://github.com/ziatdinovmax/GPim), and various Pycroscopy (https://github.com/pycroscopy/pycroscopy and https://www.github.com/pycroscopy/stemtool/) packages. All the technologies and workflows discussed during the tutorials will be open source. The attendees are encouraged to contact the organizers in advance to setup analysis of own datasets. The meeting will be free of charge. The final program will be available by November 7 and registration deadline is November 13.


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From wandaver2m-at-gmail.com Thu Oct 22 01:47:49 2020
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Third International Symposium on Advanced Microscopy and Spectroscopy
on the Occasion of Celebrating the NSF MRSEC at UCI
University of California, Irvine

November 5-7, 2020

To celebrate the establishment of the Center for Complex and Active
Materials (CCAM) - a new Materials Research Science and Engineering
Center (MRSEC) funded by National Science Foundation (NSF), UCI
Irvine Materials Research Institute (IMRI) is delighted to host a
special materials research workshop in conjunction with the Third
International Symposium on Advanced Microscopy and Spectroscopy
(ISAMS-3), on November 5-7, 2020. A workshop centered on CCAM
activities will be held on November 5, to kick off the UCI MRSEC,
followed by ISAMS-3 on November 6-7, 2020. The ISAMS-3 will bring
together the scientific community working on various aspects of
transmission electron microscopy to exchange latest research
results and ideas, and to address challenges in the development of
atomic scale imaging and spectroscopy for advancing materials and
biological sciences. Both events will be held virtually via Zoom
and are open to the scientific community. For more information
about the events, please visit: https://sites.uci.edu/isams3/.

Register here at https://sites.uci.edu/isams3/#Registration,
A Zoom link will be sent to all registered attendees by email.

The National Science Foundation has awarded $18 million to UCI
in support of the CCAM - a new NSF MRSEC. The CCAM builds on campus
strengths in multidisciplinary science and engineering research,
world-class facilities, and commitment to diversity. The primary
mission of CCAM is to establish foundational knowledge in the
science and engineering of new classes of materials offering
unique and broad functionality via an interplay among design,
simulation, synthesis, and advanced characterization.

Established in 2015, UCI IMRI is an interdisciplinary special
research program under the Office of Research, and a key enabler
of the CCAM initiative. It serves as the cross-campus nexus for
materials research in Southern California. IMRI operates a wide
range of state-of-the-art shared facilities for the analysis and
characterization of materials and devices ranging from sub- to
macroscopic length scales. The facilities are professionally
staffed, convenient, and affordable, with user-friendly services.

We are honored to invite you to join us at the special materials
research workshop and the ISAMS-3, to share with you our
accomplishments and to enable future collaborations amongst attendees.

Xiaoqing Pan
Director, IMRI and CCAM - An NSF MRSEC


==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Fri, 23 Oct 2020 18:00:22 -0500
Subject: [Microscopy] viaWWW:Stanford EM-X Webinar Series

Contents Retrieved from Microscopy Listserver Archives
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Email: afm-at-stanford.edu Name: Ann Marshall

Title-Subject: [Filtered] Stanford EM-X Webinar Series

Message: We are excited to announce the launching of the Stanford Electron Microscopy-X (EM-X)
Symposium series. The organizing co-chairs are Professors Robert Sinclair, Wah Chiu, Jennifer Dionne
and Yi Cui at Stanford University/SLAC. We would like to invite you to attend this online event.

Time: Nov. 2 (Monday), 8-9:30am California time. Speakers: Professor Jacques Dubochet (2017 Nobel
Laureate in Chemistry) University of Lausanne Talk title"A Nobel prize, how and what for?"
Professor Yi Cui (2017 Blavatnik Laureate in Physical Sciences and Engineering) Stanford
University/SLAC National Lab
Talk title"Cryo and in-situ electron microscopy for clean energy" Please register at:
https://stanford.zoom.us/webinar/register/WN_-ELnXVi1S1qgE9PlaIGeDw

The EM-X event is in on-line format. It will be delivered Iive". Each presentation is 30 min plus
15 min Q&A, with a possible panel discussion at the end. We plan to hold this symposium series
monthly. Each event will usually consist of two speakers (one in the biomedical area and the other
in physical science and engineering). Here we use "X" to indicate the breadth of EM technique
development and the many rich scientific problems EM can address. Due to the COVID-19 situation, we
believe that it is timely to initiate such a symposium series to bring the global electron
microscopy community together, to highlight exciting progress and to stimulate creative thinking for
future developments. We invite attendees internationally from academia, national labs, industry,
funding agencies and manufacturers.
Ann Marshall
TEM Manager
Stanford Nano Shared Facilities

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From: korinek-at-mcmaster.ca
Date: Sun, 25 Oct 2020 09:05:06 -0500
Subject: [Microscopy] Job opening: Canadian Centre for Electron Microscopy is looking for a

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The Canadian Centre for Electron Microscopy (CCEM), located at McMaster University in Hamilton, ON, Canada provides world-class electron microscopy capabilities and expertise to Canadian researchers and industry working in a broad range of fields. Our vision is to be one of the leading electron microscopy facilities in the world for the quality of the scientific research, to be the go-to provider of electron microscopy services to Canadian industry and to play a leading role in promoting interactions amongst researchers in various fields nationally and internationally.

The CCEM is seeking a NION microscope operator to join CCEM's research staff team. The Nion operator will be responsible for operations, trouble shooting and data acquisition of CCEM's Nion Hermes UltraSTEM transmission electron microscope which will be installed in early 2021. The position requires in-depth knowledge in the science and practical operation of the instrument, knowledge of the physics of monochromated low-loss electron energy loss spectroscopy as well as a strong background in data processing and hardware scripting using Python. Additionally, excellent skills in communicating and working collaboratively with team members and partners are required.
This position reports to the Facilities Manager of the CCEM.

Assets:
- Participation and leadership in scientific projects
- Processing of data into scientific results
- Development of custom acquisition and processing methods using the NION API and Python
- Participate/lead publishing results in peer-reviewed journals
- Expert knowledge in operating a Nion microscope
- Acquisition and processing of monchromated electron energy loss spectroscopy data
- Ability to troubleshoot and service the microscope with help of NION support
- Keeping informed about new techniques and instrumentation
- Dealing with vendors in consistent manner
- Must be able to collaboratively work in a team and with external partners/customers
- Must be able to plan concurrent projects effectively
- Must be able to stay within the allocated resources (time, materials) for a given task
- Represents CCEM's vision and mission in a positive way to partners and customers
- Teaches operations and theory of microscopy to others
- PhD in Physics, Engineering or related fields a strong asset
- Strong track record of publications an asset
Additional Information
This position is initially limited to 2 years, with the possibility of renewal.

To apply for this position, please use the following link: https://careers.mcmaster.ca/psp/prepprd/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_JBPST&Action=U&FOCUS=Applicant&SiteId=1001&JobOpeningId=34884&PostingSeq=1

Andreas Korinek,PhD
Acting Executive Director
Canadian Centre for Electron Microscopy
1280 Main St W
ABB B161
Hamilton, ON, L8S 4M1
location:ABB B161
phone:+1 905 525 9140 x 20400
email:korinek-at-mcmaster.ca
web:http://ccem.mcmaster.ca


McMaster University| Brighter World




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Date: Thu, 29 Oct 2020 06:21:41 -0500
Subject: [Microscopy] viaWWW:size distribution for negatively stained exosomes

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Email: ralvaradojr-at-ufl.edu Name: Rudy Alvarado

Title-Subject: [Filtered] HELP! Processing Spleen FFPE tissue for Immunoelectron Microscopy

Message: Hi everyone,
I am in need of help.
I'm trying to process spleen tissue that was formalin-fixed paraffin embedded. I have processed this
tissue twice, and I had the same results. The tissue get embedded by HM20 resin with Z6040 embedding
primer, however, when I sectioned the tissue, it crumpled as if it was not properly infiltrated.
To process the FFPE tissue, I excised 1-2 mm2 core punches from the FFPE blocks. I did extensive
xylene washes (7-10 washes in 3-5 days) on a rocker. The tissue was reverse processed with the aid
of a microwave processor: 5-6x 100% EtOH, 75% EtOH, 50% EtOH, 2x water washes, 1x PBS wash, fixed in
4% PFA/0.5% GA in PBS (overnight on shaker), 3-5x PBS washes, agarose encapsulated one of the
samples (sample #1), PBS wash, 2x water washes, short EtOH dehydration series, one of the samples
was en bloc treated in 2% UA in 75% EtOH (sample #2), 2x anhydrous EtOH washes, 50% resin (anhydrous
acetone: HM20 resin), 6-10x 100% resin exchanges (during 5-7 days; no MW was used; tissue was kept
in 4 deg C during the exchange on a shaker at 100 RPM). When I transferred the pieces to flat bottom
capsules or Eppendorf tubes, I added some nitrogen gas to remove any oxygen present and then cap the
tubes. I placed the samples in a freeze sub unit and uv cold cured at - 20 deg C for at least 48
hours. Lastly, I did use a new HM20 kit.
I know this was overkill, but if anyone has any suggestions, I very much appreciate it. I
successfully processed FFPE lung and intestine tissue before.
Thank you,
Rudy

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7, 54 -- Subject: viaWWW: HELP! Processing Spleen FFPE tissue for Immunoelectron
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From fantjame060utg-at-gmail.com Tue Oct 27 06:49:54 2020
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Message-ID: {A68D20A3.DF9BF432-at-gmail.com}

Hi Rudy!

My suggestion would be to stop wasting time and resources making EM with FFPE samples.
Formalin contains methanol and histology fixation leads to heavy artifacts like protein precipitation, sample shrinkage etc.

In EM you'll get a high resolution view of something very far from native state, what's the point?
In short: you'll generate results but absolutely not meaningful.

My 2 cents

Best regards,
Stephane







On Monday, October 26, 2020, 11:15:13 PM GMT+1, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:








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Email: ralvaradojr-at-ufl.edu Name: Rudy Alvarado

Title-Subject: [Filtered] HELP! Processing Spleen FFPE tissue for Immunoelectron Microscopy

Message: Hi everyone,
I am in need of help.
I'm trying to process spleen tissue that was formalin-fixed paraffin embedded. I have processed this
tissue twice, and I had the same results. The tissue get embedded by HM20 resin with Z6040 embedding
primer, however, when I sectioned the tissue, it crumpled as if it was not properly infiltrated.
To process the FFPE tissue, I excised 1-2 mm2 core punches from the FFPE blocks. I did extensive
xylene washes (7-10 washes in 3-5 days) on a rocker. The tissue was reverse processed with the aid
of a microwave processor: 5-6x 100% EtOH, 75% EtOH, 50% EtOH, 2x water washes, 1x PBS wash, fixed in
4% PFA/0.5% GA in PBS (overnight on shaker), 3-5x PBS washes, agarose encapsulated one of the
samples (sample #1), PBS wash, 2x water washes, short EtOH dehydration series, one of the samples
was en bloc treated in 2% UA in 75% EtOH (sample #2), 2x anhydrous EtOH washes, 50% resin (anhydrous
acetone: HM20 resin), 6-10x 100% resin exchanges (during 5-7 days; no MW was used; tissue was kept
in 4 deg C during the exchange on a shaker at 100 RPM). When I transferred the pieces to flat bottom
capsules or Eppendorf tubes, I added some nitrogen gas to remove any oxygen present and then cap the
tubes. I placed the samples in a freeze sub unit and uv cold cured at - 20 deg C for at least 48
hours. Lastly, I did use a new HM20 kit.
I know this was overkill, but if anyone has any suggestions, I very much appreciate it. I
successfully processed FFPE lung and intestine tissue before.
Thank you,
Rudy

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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] size distribution for negatively stained exosomes.

Message: Hi,
I am trying to measure the size distribution for the negatively stained exosome particles. But the
background is unevenly stained, which makes it difficult to set the threshold. Can anyone please
suggest any tool where I can select individual particles and perform the analysis?
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From: stefan.diller-at-t-online.de
Date: Thu, 29 Oct 2020 07:02:45 -0500
Subject: [Microscopy] Spirulina SEM protocol

Contents Retrieved from Microscopy Listserver Archives
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Dear All,

I am looking for a protocol to critical-point-dry spirulina algae grown on glass substrates.

Normally I would go directly into 2% Glutaraldehyde for fixation.

Or would you recommend using a buffer first?

Next steps would be postfix with 2% OsO4, washing and the Ethanol up to 100%.

Any other recommendations?


Thanks,

Stefan


--


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Stefan Diller - Scientific Photography
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Websites:
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Anfahrt: http://Mail.map24.com/Stefan.Diller
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15, 24 -- Subject: Spirulina SEM protocol
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From: sergei2-at-ornl.gov
Date: Thu, 29 Oct 2020 12:57:41 -0500
Subject: [Microscopy] Abstract submissions: AI in (S)TEM

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Dear colleagues
We would like to invite you to submit an abstract for the virtual school "AI for atoms: How to machine learn (S)TEM" to be held at ORNL, December 7-10, 2020 organized by Maxim Ziatdinov, Rama Vasudevan, Debangshu Mukherjee, and Sergei V. Kalinin. The contributed talks will be selected on the topics merging (S)TEM/EELS/4DSTEM with quantification and ML.
The school will feature the combination of invited talks from leading experts in physics-applied machine learning and electron microscopy, including Paul Voyles (U Wisc), Viren Jain and Peter Battaglia (Google), Colin Ophus (Berkeley), Shirley Ho (Flatiron institute), Pinshane Huang (UIUC), A.G. Wilson (New York University) and contributed talks selected from submitted abstracts, as well as hands-on tutorials for AtomAI, GPim, and PyCroscopy packages. Please register at the https://www.surveymonkey.com/r/257WD3B.
Submit the abstracts by November 3 via e-mail directly to Sergei Kalinin (sergei2-at-ornl.gov). The second call for the school is provided below, and looking forward to (virtually) meeting you at Oak Ridge!
Sergei V. Kalinin


Second call:
Machine learning (ML) has emerged as a powerful tool for data and image analysis and as an enabling component of autonomous systems in areas ranging from biological and medical imaging to self-driving cars. This rapid growth in ML applications poses the question as to which of these methods can be applied in electron microscopy, and perhaps more importantly, what insights into the physics and chemistry of real materials can they yield. This virtual school on AI for atoms: how to machine learn (S)TEM, to be held December 7-10, will combine invited and contributed presentations at the forefront of ML applications in Scanning Transmission Electron Microscopy (STEM) and Transmission Electron Microscopy (TEM), Electron Energy Loss Spectroscopy (EELS), and 4D STEM, as well as for physics and chemistry extraction from STEM data sets. The school will feature the combination of invited talks from leading experts in physics-applied machine learning and electron microscopy, including Paul Voyles (U Wisc), Viren Jain and Peter Battaglia (Google), Colin Ophus (Berkeley), Shirley Ho (Flatiron institute), Pinshane Huang (UIUC), A.G. Wilson (New York University) and contributed talks selected from submitted abstracts. It will further feature tutorials on recent developments in ML analysis of mesoscopic and atomically resolved images and spectroscopy in STEM, including classical graph analysis of STEM data, deep convolutional neural networks (DCNNs) for feature identifications, symmetry-invariant (variational) autoencoders ((V)AE), and Gaussian Processes based super-resolution imaging and image reconstruction. The tutorials will be followed by the hands-on tutorial sessions introducing the attendees to the AtomAI (https://github.com/pycroscopy/atomai), GPim (https://github.com/ziatdinovmax/GPim), and various Pycroscopy (https://github.com/pycroscopy/pycroscopy and https://www.github.com/pycroscopy/stemtool/) packages. All the technologies and workflows discussed during the tutorials will be open source. The a!
ttendees
are encouraged to contact the organizers in advance to setup analysis of own datasets. The meeting will be free of charge. The final program will be available by November 7 and registration deadline is November 13.


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From: antoniom-at-ictan.csic.es
Date: Thu, 29 Oct 2020 17:55:01 -0500
Subject: [Microscopy] Re: Spirulina SEM protocol

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

What about using cryo-SEM? In many cases, it avoids structural
distorsions associated to drying and other artifacts, with almost no
preparation work.

Best regards,

Antonio D. Molina-Garcia
ICTAN-CSIC
Madrid, Spain



stefan.diller-at-t-online.de escribió:

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} Dear All,
}
} I am looking for a protocol to critical-point-dry spirulina algae
} grown on glass substrates.
}
} Normally I would go directly into 2% Glutaraldehyde for fixation.
}
} Or would you recommend using a buffer first?
}
} Next steps would be postfix with 2% OsO4, washing and the Ethanol up to 100%.
}
} Any other recommendations?
}
}
} Thanks,
}
} Stefan
}
}
} --
}
}
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} Anfahrt: http://Mail.map24.com/Stefan.Diller
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}
} ==============================Original Headers==============================
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} 15, 24 -- Subject: Spirulina SEM protocol
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From: microscopy.listserver-at-gmail.com
Date: Fri, 30 Oct 2020 08:55:39 -0500
Subject: [Microscopy] viaWWW:Free Webinars - 3D Panfocal Microscopy

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From: benada-at-biomed.cas.cz
Date: Wed, 4 Nov 2020 19:12:16 -0600
Subject: [Microscopy] Re: [SPAM] viaWWW:size distribution for negatively

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Hello Ravi,
It all depends on the quality of negative staining. What kind of
support film did you use, carbon or carbon/formvar? Did you use a glow
discharge?
In our hands, ~1% ammonium molybdate with about 0.1% trehalose worked
well on glow discharge treated carbon/formvar grids for negative
staining of exosome particles. Ammonium molybdate produces lower
contrast than uranyl salts, but it can make evenly spread negative
stain.

Regards

Oldrich

--
Oldřich Benada
Institute of Microbiology, Czech Acad. Sci.
Laboratory of Molecular Structure Characterization
Electron Microscopy Group
Vídeňská 1083
142 20 Prague 4
Czech Republic

On Thu, 29 Oct 2020 06:23:06 -0500, microscopy.listserver-at-gmail.com
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} Title-Subject: [Filtered] size distribution for negatively stained
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} Message: Hi,
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From: rstern-at-ualberta.ca
Date: Sun, 8 Nov 2020 15:27:06 -0600
Subject: [Microscopy] SEM-scintillator vs. semiconductor BSE detector performance

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

Our rather old (27 years and counting) 2010 TEM has developed a problem.

I came in yesterday and it had completely shut down.. Up until yesterday it had been working well. Checking the records, there were no power issues (we have a UPS plus generator backed up power), no cooling water issues, no gas (solenoid or insulating) issues, and no room temperature issues either. As it had completely shut down there were no alarms showing either. A quick investigation showed that the main circuit breaker on the PS had activated.

Resetting this enabled the TEM to restart, but after around 30 seconds the breaker activated again and the TEM shut down. But, the actual breaker switch doesn't drop fully to the open position. The only way that happens it to manually push the breaker down. I have tried a few times and always it shuts down, but the breaker never drops fully. Could it be a faulty breaker?

I was wondering if anyone out there has experienced a similar issue and may have any clues.

I realise that there may be a multitude of reasons, but any hint would be useful.

Thank you very much in advance!!

Kind regards and stay safe.

Colin Veitch

Manager, Microscopy - Waurn Ponds Laboratory CSIRO Manufacturing

E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057 Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia.
www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx

CSIRO acknowledges the Traditional Owners of the lands that we live and work on across Australia and pays its respect to Elders past, present and emerging.



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From fritschemichael755-at-gmail.com Fri Nov 6 06:52:39 2020
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Message-ID: {2B9C7D75.C8E814D1-at-gmail.com}

We are a mineral isotope lab with a Zeiss EVO MA15 SEM (LaB6) used in a
supporting role to characterize mineral compositional contrast (often very
subtle) with backscattered electrons, normally at high vacuum, high kV, and
beam currents up to 5 nA. BSE detection currently utilizes Zeiss' 4
quadrant semiconductor detector under the pole piece. I'm interested to
hear from those who have experience using a YAG scintillator detector for
similar purposes, either the one made by Zeiss or others, and particularly
if you've any evidence of their relative sensitivity to compositional
contrast. I'm led to believe that the YAG detector is more sensitive than
the semiconductor type at the analytical conditions mentioned above (high
vacuum, high kV). What do the users have to say? Do any publications exist
in which the YAG detector performance is objectively evaluated?

Thanks and best regards,


Richard Stern
Managing Director, CCIM (SIMS facility)
Department of Earth & Atm Sciences
University of Alberta
116 St. & 85 Ave.
Edmonton, AB T6G 2R3
Canada

780-248-1063 (lab)



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From: diller-at-stefan-diller.com
Date: Thu, 12 Nov 2020 11:07:47 -0600
Subject: [Microscopy] Quanta 200 FEG PUC communication error

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear All,

at a friend`s Quanta 200 FEG-SEM a message is shown:

"Communication Problems with controller PUC"

and the TAD test shows at "CAN Tests" a problem with FGSU and VCB.

Anyone out there who knows if this is a hardware problem or more a software problem?

Even after a cold restart the scope comes back with the same errors.


Best wishes,

Stefan

--


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Arndtstrasse 22
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Websites:
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www.stefan-diller.com
www.electronmicroscopy.info
www.elektronenmikroskopie.info
www.zwillingsprojekt.de
www.assisi.de
Anfahrt: http://Mail.map24.com/Stefan.Diller
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From: sergei2-at-ornl.gov
Date: Thu, 12 Nov 2020 12:54:27 -0600
Subject: [Microscopy] AI in STEM - program/last day registration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues - as a reminder, this Thursday-Friday are the last days to register for the virtual school "AI for atoms: How to machine learn STEM" to be held at ORNL, December 7-10. The registration site is https://www.surveymonkey.com/r/257WD3B.

Please contact Sergei Kalinin (sergei2-at-ornl.gov) to submit the abstract. The tentative program is provided below. Given the recent progress in the field, the open invited and contributed talks will be selected from submitted abstracts.

Looking forward to (virtually) meeting you at Oak Ridge!

Sergei

Tentative program

December 7.
9.30 am - 9.45 am Opening presentation and workshop plan
9.45 am - 10.30 am, Max Tegmark (MIT), TBA
10.30 am - 11.00 am, Paul Voyles (U. Wisc.) Supervised and Unsupervised Machine Learning for 4D STEM Data
11.00 am - 11.30 am, Pinshane Huang (U. Illinois) Probing the Strain Fields of Single-Atom Defects via Deep Learning
11.30 am - 11.45 am Contributed talk 1
11.45 am - 12.00 am Contributed talks 2
12.00 am - 12.30 pm Peter Battaglia (Google) Learning simulation using structured models

1.30 pm - 4.00 pm Tutorial 1: Intro and semantic segmentation of images
* Intro to NN in PyTorch: Regression
* Intro to NN in PyTorch: Image-level classification (+ visualization of activation maps, etc.)
* Semantic segmentation: a basic example in Pytorch and transition to AtomAI.


December 8
10.00 am - 10.30 am, Colin Ophus (Berkeley) Computational Imaging, data analysis, and machine learning in STEM and TEM
10.30 am - 11.00 am, Shirley Ho (Flatiron Institute) Discovering symbolic equations using deep learning
11.00 am - 11.30 am, Andrew G. Wilson, (NYU) Learning Symmetries in Neural Networks
11.30 am - 11.45 am Contributed talk 1
11.45 am - 12.00 am Contributed talks 2
1200 pm - 12.30 pm Invited talk

1.30 pm - 4.00 pm Tutorial 2: Autoencoders
* Autoencoders for data cleaning
* Im2spec encoder-decoder networks
* Variational and invariant autoencoders

December 9
10.00 am - 10.30 am Vipin Kumar (UMN), Physics-Guided Machine Learning: A New Framework for Accelerating Scientific Discovery
10.30 am - 11.00 am Magnus Nord (NTNU) Processing very large datasets using pyXem: STEM-DPC of ferromagnetic domains
11.00 am - 11.30 am, Gerd Duscher (UTK) How can STEM be quantitative?
11.30 am - 11.45 am Contributed talk 1
11.45 am - 12.00 am Contributed talks 2
1200 pm - 12.30 pm Invited talk

1.30 pm - 4.00 pm Tutorial 3: Probabilistic ML
* Gaussian processes
* Bayesian neural networks
* Bayesian optimization (Ising model, dummy experiment)

December 10
10.00 am - 10.30 am Robert Howden, Maximum resolution from the Ronchigram: human vs. deep learning
10.30 am - 11.00 am Martin Jankowiak (Broad Institute) Bayesian methods for adaptive experimental design
11.00 am - 11.30 am, Dhiresha Kudithipudi (UTSA), Task Agnostic Lifelong Learning
11.30 am - 11.45 am Contributed talk 1
11.45 am - 12.00 am Contributed talk 2
12.00 pm - 12.30 pm Viren Jain (Google) Synapse-resolution connectomics in fly, bird, and human brains

1.30 pm - 4.00 pm Tutorial 4: PyCroscopy and STEM tools


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From: Colin.Veitch-at-csiro.au
Date: Thu, 12 Nov 2020 17:59:14 -0600
Subject: [Microscopy] JEOL JEM 2010 problem solved

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi,

Thank you all for your suggestions on possible causes for the circuit breaker opening on my 2010.

The problem has been resolved and was relatively straightforward.

The facilities people (unknown to me) turned the temperature of the cooling water down to around 16 degrees C. This was below the dew point of the lab for a few vey humid days. Water condensed onto the diffusion pump (and other places) and from there dripped into the diffusion pump heater shield. This caused a short which threw the breaker when it was powered up (~ 30 s after initial start-up).

The cooling water temperature has been changed and all the parts dried out. It is now back on line and running fine.

Kind regards and stay safe.

Colin Veitch

Manager, Microscopy - Waurn Ponds Laboratory
CSIRO Manufacturing

E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057
Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia.
www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx

CSIRO acknowledges the Traditional Owners of the lands that we live and work on across Australia and pays its respect to Elders past, present and emerging.



PLEASE NOTE
The information contained in this email may be confidential or privileged. Any unauthorised use or disclosure is prohibited. If you have received this email in error, please delete it immediately and notify the sender by return email. Thank you. To the extent permitted by law, CSIRO does not represent, warrant and/or guarantee that the integrity of this communication has been maintained or that the communication is free of errors, virus, interception or interference.

Please consider the environment before printing this email.

Hi,

Our rather old (27 years and counting) 2010 TEM has developed a problem.

I came in yesterday and it had completely shut down.. Up until yesterday it had been working well. Checking the records, there were no power issues (we have a UPS plus generator backed up power), no cooling water issues, no gas (solenoid or insulating) issues, and no room temperature issues either. As it had completely shut down there were no alarms showing either. A quick investigation showed that the main circuit breaker on the PS had activated.

Resetting this enabled the TEM to restart, but after around 30 seconds the breaker activated again and the TEM shut down. But, the actual breaker switch doesn't drop fully to the open position. The only way that happens it to manually push the breaker down. I have tried a few times and always it shuts down, but the breaker never drops fully. Could it be a faulty breaker?

I was wondering if anyone out there has experienced a similar issue and may have any clues.

I realise that there may be a multitude of reasons, but any hint would be useful.

Thank you very much in advance!!

Kind regards and stay safe.

Colin Veitch


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From: microscopy.listserver-at-gmail.com
Date: Fri, 13 Nov 2020 06:36:09 -0600
Subject: [Microscopy] Fwd: O-rings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


X-from: Frank Karl {frank_karl-at-ardl.com}
Hello Everyone, I have just used my last 0-ring on the sample rod for our FEI CM12. We are no
longer covered by their service contract and I need to find a replacement source. I thought I had a
life time supply, but it seems I'm wrong.
I believe the O-ring is Viton but their 11 digit number doesn't mean anything outside of the FEI
walls. Does anyone have any information on size, perhaps a catalog number for a company from which
I can order from? Thanks!!!




Stay calm...Be brave....watch for signs

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305


==============================Original Headers==============================
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From: microscopy.listserver-at-gmail.com
Date: Fri, 13 Nov 2020 06:36:59 -0600
Subject: [Microscopy] Fwd: AI in STEM - program/last day registration

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Kalinin, Sergei V. {sergei2-at-ornl.gov}

Dear colleagues - as a reminder, this Thursday-Friday are the last days to register for the virtual
school "AI for atoms: How to machine learn STEM" to be held at ORNL, December 7-10. The registration
site is https://www.surveymonkey.com/r/257WD3B.
Please contact Sergei Kalinin (sergei2-at-ornl.gov) to submit the abstract. The tentative program is
provided below. Given the recent progress in the field, the open invited and contributed talks will
be selected from submitted abstracts.
Looking forward to (virtually) meeting you at Oak Ridge!

Sergei

Tentative program

December 7. 9.30 am - 9.45 am Opening presentation and workshop plan
9.45 am - 10.30 am, Max Tegmark (MIT), TBA 10.30 am - 11.00 am, Paul Voyles (U. Wisc.) Supervised
and Unsupervised Machine Learning for 4D STEM Data
11.00 am - 11.30 am, Pinshane Huang (U. Illinois) Probing the Strain Fields of Single-Atom Defects
via Deep Learning
11.30 am - 11.45 am Contributed talk 1
11.45 am - 12.00 am Contributed talks 2
12.00 am - 12.30 pm Peter Battaglia (Google) TBA
1.30 pm - 4.00 pm Tutorial 1: Intro and semantic segmentation of images
* Intro to NN in PyTorch: Regression
* Intro to NN in PyTorch: Image-level classification (+ visualization of activation maps, etc.)
* Semantic segmentation: a basic example in Pytorch and transition to AtomAI.


December 8
10.00 am - 10.30 am, Colin Ophus (Berkeley) Computational Imaging, data analysis, and machine
learning in STEM and TEM
10.30 am - 11.00 am, Shirley Ho (Flatiron Institute) Discovering symbolic equations using deep
learning 11.00 am - 11.30 am, Andrew G. Wilson, (NYU) Learning Symmetries in Neural Networks
11.30 am - 11.45 am Contributed talk 1
11.45 am - 12.00 am Contributed talks 2
1200 pm - 12.30 pm Invited talk

1.30 pm - 4.00 pm Tutorial 2: Autoencoders
* Autoencoders for data cleaning
* Im2spec encoder-decoder networks
* Variational and invariant autoencoders

December 9
10.00 am - 10.30 am Vipin Kumar (UMN), Physics-Guided Machine Learning: A New Framework for
Accelerating Scientific Discovery 10.30 am - 11.00 am Magnus Nord (NTNU) Processing very large
datasets using pyXem: STEM-DPC of ferromagnetic domains
11.00 am - 11.30 am, Gerd Duscher (UTK) How can STEM be quantitative?
11.30 am - 11.45 am Contributed talk 1
11.45 am - 12.00 am Contributed talks 2
1200 pm - 12.30 pm Invited talk

1.30 pm - 4.00 pm Tutorial 3: Probabilistic ML
* Gaussian processes
* Bayesian neural networks
* Bayesian optimization (Ising model, dummy experiment)

December 10
10.00 am - 10.30 am Robert Howden, Maximum resolution from the Ronchigram: human vs. deep learning
10.30 am - 11.00 am Martin Jankowiak (Broad Institute) Bayesian methods for adaptive experimental design
11.00 am - 11.30 am, Dhiresha Kudithipudi (UTSA), Task Agnostic Lifelong Learning
11.30 am - 11.45 am Contributed talk 1
11.45 am - 12.00 am Contributed talk 2 12.00 pm - 12.30 pm Viren Jain (Google) Synapse-resolution
connectomics in fly, bird, and human brains

1.30 pm - 4.00 pm Tutorial 4: PyCroscopy and STEM tools

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From: microscopy.listserver-at-gmail.com
Date: Fri, 13 Nov 2020 07:31:19 -0600
Subject: [Microscopy] viaWWW: IEEE PAINE 2020-Call for Participation

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From: vitaly-at-sia-cam.com
Date: Fri, 13 Nov 2020 10:53:23 -0600
Subject: [Microscopy] Re: Fwd: O-rings

Contents Retrieved from Microscopy Listserver Archives
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viton
2mm wide
8mm ID
12mm OD

A good source among many:
https://www.mcmaster.com/o-rings/width~2-000mm/material~viton-fluoroelastomer-rubber/od~12mm/

Vitaly Feingold
SIA
2773 Heath Lane
Duluth GA 30096
Ph. 770-232-7785
Fax 770-232-1791
www.sia-cam.com
vitaly-at-sia-cam.com

On 11/13/2020 7:39 AM, microscopy.listserver-at-gmail.com wrote:
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} X-from: Frank Karl {frank_karl-at-ardl.com}
} Hello Everyone, I have just used my last 0-ring on the sample rod for our FEI CM12. We are no
} longer covered by their service contract and I need to find a replacement source. I thought I had a
} life time supply, but it seems I'm wrong.
} I believe the O-ring is Viton but their 11 digit number doesn't mean anything outside of the FEI
} walls. Does anyone have any information on size, perhaps a catalog number for a company from which
} I can order from? Thanks!!!
}
}
}
}
} Stay calm...Be brave....watch for signs
}
} Frank Karl
} Microscopist
} Akron Rubber Development Laboratory
} 2887 Gilchrist Road
} Akron, Ohio 44305
}
}
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5, 45 -- Subject: Re: [Microscopy] Fwd: O-rings
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5, 45 -- References: {202011131239.0ADCdAx4024812-at-microscopy.com}
5, 45 -- From: Vitaly Feingold {vitaly-at-sia-cam.com}
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From: microscopy.listserver-at-gmail.com
Date: Fri, 13 Nov 2020 17:23:46 -0600
Subject: [Microscopy] Fwd: Virtual Cryo Microscopy Group annual meeting 18/19 November 2020

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



X-from: Raffaella Carzaniga {raffaella.carzaniga-at-crick.ac.uk}



Dear colleagues,

This year the annual Cryo Microscopy Group meeting will take place virtually on two consecutive
afternoons.

On Wednesday 18th November, the talks are mostly TEM related with expert talks on Cryo Electron
Tomography, MicroED and the exciting new technique of Cryo Ptychography.

On Thursday 19th November, we have talks on experimentation with cooled micromanipulators in SEM ,
CryoSEM in food science and developments at B24 Beamline at Diamond in Cryo X-ray and Cryo Super
Resolution Microscopy

There are also going to be opportunities to engage with the trade representatives using break-out
rooms to try to simulate the live meeting experience.

Full programme details herehttp://www.cryomicroscopygroup.org.uk/CMG2020.php
{https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.cryomicroscopygroup.org.uk%2FCMG2020.php&data=04%7C01%7C%7Ca3d1b5ffcf7c4c032c1808d88724a974%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C0%7C637407938815688566%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=dhhwhjkaFpk%2Fm31BT6jMR1F3%2BQJsSDPm%2B%2FY1AShZhBM%3D&reserved=0}

You don’t need to register - just join us using the Zoom link and passcode.

Time: Nov 18, 2020 02:00 PM London

        Every day, until Nov 19, 2020, 2 occurrence(s)

        Nov 18, 2020 02:00 PM

        Nov 19, 2020 02:00 PM

Please download and import the following iCalendar (.ics) files to your calendar system.

Daily:https://crick.zoom.us/meeting/u5IkcuGsqzwtHtBFPqmbEYnwVDZIyGMKkU7E/ics?icsToken=98tyKu-upz4iHtGStx2DR_MAHY_4WejwmGZagrd_ui_0DiJ2Ww3hAMZ4G6tYPfPb
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Join Zoom Meeting

https://crick.zoom.us/j/66958457744?pwd=Q1gxV0xxTS80eitla2plZmJ0LzVOUT09
{https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fcrick.zoom.us%2Fj%2F66958457744%3Fpwd%3DQ1gxV0xxTS80eitla2plZmJ0LzVOUT09&data=04%7C01%7C%7Ca3d1b5ffcf7c4c032c1808d88724a974%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C0%7C637407938815698558%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=aacui0okskXGqp1345nZ52E6rnp64Kjr8HquusOv0L0%3D&reserved=0}

Meeting ID: 669 5845 7744

Passcode: 382236

One tap mobile

+442080806591,,66958457744#,,,,,,0#,,382236# United Kingdom

+442080806592,,66958457744#,,,,,,0#,,382236# United Kingdom

Dial by your location

        +44 208 080 6591 United Kingdom

        +44 208 080 6592 United Kingdom

        +44 330 088 5830 United Kingdom

        +44 131 460 1196 United Kingdom

        +44 203 481 5237 United Kingdom

        +44 203 481 5240 United Kingdom

        +44 203 901 7895 United Kingdom

Meeting ID: 669 5845 7744

Passcode: 382236

Find your local number:https://crick.zoom.us/u/cW6T8wJom
{https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fcrick.zoom.us%2Fu%2FcW6T8wJom&data=04%7C01%7C%7Ca3d1b5ffcf7c4c032c1808d88724a974%7C4eed7807ebad415aa7a99170947f4eae%7C0%7C0%7C637407938815698558%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C1000&sdata=ALo9bbL9UD%2F%2FEUckypwFqK9jpAbTM735TOKs6awqqHg%3D&reserved=0}

Join by SIP

66958457744-at-zoomcrc.com {mailto:66958457744-at-zoomcrc.com}

Join by H.323

213.19.144.110 (Amsterdam Netherlands)

213.244.140.110 (Germany)

207.226.132.110 (Japan)

Meeting ID: 669 5845 7744

Passcode: 382236

Please spread the word and we are looking forward to you joining us.

Raffa

Raffaella Carzaniga PhD

Deputy Head of Electron Microscopy

The Francis Crick Institute

1 Midland Road

London

NW1 1AT

UK

T: +44 (0) 20379 61732

E: Raffaella.carzaniga-at-crick.ac.uk {mailto:Raffaella.carzaniga-at-crick.ac.uk}

W: www.crick.ac.uk {http://www.crick.ac.uk/}

The Francis Crick Institute Limited is a registered charity in England and Wales no. 1140062 and a
company registered in England and Wales no. 06885462, with its registered office at 1 Midland Road
London NW1 1AT


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58, 53 -- Subject: Fwd: Virtual Cryo Microscopy Group annual meeting 18/19 November 2020
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From: microscopy.listserver-at-gmail.com
Date: Fri, 13 Nov 2020 17:25:11 -0600
Subject: [Microscopy] Fwd: Fwd: O-rings

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


X-from: V.Ray {vray-at-partbeamsystech.com}

Hi Frank,

O-rings are generic supply and you would be just fine with Viton material for TEM holder.

To find correct size - first measure diameter of the O-ring thread and then find closest size in a
standard chart, AS568 or metric. Charts are widely available online, and if you have difficulty
finding exact match then order couple of close sizes - the cost is literally pennies so just toss
away the wrong size and use what fits.

There are *hundreds* places to order viton O-rings online, from generics like RubberStore where I
get large quantities for pennies, to Duniway Stockroom where rubber gaskets and O-rings are cleaned,
degassed in vacuum oven, and packaged for immediate use.

A word of caution - if you buy o-rings from a general rubbers supply outlet then keep in mind that
they likely have been touched by hands, didn't go through critical cleaning, and weren't packaged in
a cleanroom environment. They also may have seams along the edge from manufacturing. Thus you have
to inspect and clean them, but difference in price is absolutely worth it.

Best Wishes,
Valery

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
www.partbeamsystech.com
www.fibsemproducts.com
www.freudlabs.com

"Only the Paranoid Survive" (A.Grove & SpaceX QA)

On 11/13/2020 7:36 AM, microscopy.listserver-at-gmail.com wrote:
} ----------------------------------------------------------------------------
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} X-from: Frank Karl {frank_karl-at-ardl.com}
} Hello Everyone, I have just used my last 0-ring on the sample rod for our FEI CM12. We are no
} longer covered by their service contract and I need to find a replacement source. I thought I had a
} life time supply, but it seems I'm wrong.
} I believe the O-ring is Viton but their 11 digit number doesn't mean anything outside of the FEI
} walls. Does anyone have any information on size, perhaps a catalog number for a company from which
} I can order from? Thanks!!!
}
}
}
}
} Stay calm...Be brave....watch for signs
}
} Frank Karl
} Microscopist
} Akron Rubber Development Laboratory
} 2887 Gilchrist Road
} Akron, Ohio 44305
}
}
} ==============================Original Headers==============================
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From: koeck-at-kth.se
Date: Sun, 15 Nov 2020 02:24:11 -0600
Subject: [Microscopy] polarity of lenses

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Hi.

Does anybody know how the magnetic fields produced by neighbouring lenses in a TEM are usually oriented.

Do the fields have the same or opposite polarity?

I'm especially interested in the OL, mini OL and 1.IL in a Jeol2100.

All the best,

Philip

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From: koeck-at-kth.se
Date: Mon, 16 Nov 2020 04:08:11 -0600
Subject: [Microscopy] magnetic field in the column of a TEM

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Hi.

Can anyone give an estimate of the magnetic field outside the actual lenses in a typical TEM?

In particular I'm interested in the plane of the SAD-aperture, from the optical axis to the wall of the column.

A rough estimate of the strength and also some idea about the orientation of the field would be interesting.

All the best,

Philip

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From: microscopy.listserver-at-gmail.com
Date: Wed, 18 Nov 2020 16:50:53 -0600
Subject: [Microscopy] viaWWW:

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Title-Subject: [Filtered] Researcher/Scientist III Position Opening, University of Arizona

Message: Dear Colleagues,

I would like to draw your attention to an open position for a staff scientist position in The Kuiper
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} From the job posting:

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From: microscopy.listserver-at-gmail.com
Date: Wed, 18 Nov 2020 16:51:42 -0600
Subject: [Microscopy] viaWWW: Ask A Microscopist Webinar

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StC is excited to announce that any MSA member who attends all webinars in this years Webinar
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From: microscopy.listserver-at-gmail.com
Date: Wed, 18 Nov 2020 16:52:21 -0600
Subject: [Microscopy] viaWWW:how to crop image in Gatan Digital Micrograph?

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Email: ravi.thakkar369-at-gmail.com Name: Ravi
Title-Subject: [Filtered] how to crop image in Gatan Digital Micrograph?

Message: Hi,
Can anyone please help me to crop the TEM images on Gatan Digital Micrograph?

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From: louise.jensen-at-epfl.ch
Date: Thu, 19 Nov 2020 07:58:26 -0600
Subject: [Microscopy] Job opening, lab manager, cryoSEM, EPFL

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Dear Colleagues,
I am leaving my job in beautiful Switzerland and I would like to draw your attention to the job posting for my replacement as a lab manager for a small lab with a CryoSEM (Gemini500 SEM w low vac, low-kV ion gun for polishing cryo samples, EDX, STEM) , a table-top SEM and a cryoSEM prep suite from Leica (UC7, ACE600, VCT500 and loading station). The lab is integrated in the Center for Advanced Surface Analysis, which is a platform shared between EPFL and UNIL in Lausanne, Switzerland. There are several large and excellent EM facilities to collaborate with in close vicinity and plenty of possibilities for technical development.
The position is permanent.
https://recruiting.epfl.ch/Vacancies/1571/Description/2?fbclid=IwAR1RobCCQvND-CNbEAGzDlElK-JHF640LKM1Pwa3eHQp_V13vaS4UZurIyA
Best wishes,
Louise.

________________________________
Louise Helene Sgaard Jensen
Lab manager, cryo-SEM
LGB EPFL
Quartier UNIL-Mouline
Btiment Gopolis
CH-1015 Lausanne Switzerland
Phone +41 21 692 44 22





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From: ielbaggari-at-rowland.harvard.edu
Date: Thu, 19 Nov 2020 09:38:48 -0600
Subject: [Microscopy] Post-doc: cryogenic STEM of quantum materials

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues,

My lab an available postdoc position in the field of atomic-resolution cryogenic scanning transmission electron microscopy (cryo-STEM) of quantum materials. The newly formed lab has unique in situ capabilities at the interdisciplinary Rowland Institute at Harvard (https://www2.rowland.harvard.edu/) and offers exciting and collaborative research projects that aim to simultaneously image and manipulate materials with exotic quantum ground states.

The candidate will benefit from close contact with the PI at the interdisciplinary Rowland Institute and engagement with the broad quantum research community at Harvard University. Our lab has a dedicated in situ cryogenic/electrical TEM holder for custom low temperature experiments, a 1.5K/9T cryostat system for ex situ characterization of imaged devices, and a suite of sample preparation and characterization tools. For microscopy experiments, we use the aberration-corrected electron microscopes (JEOL ARM 200F, forthcoming Hitachi HF-3300), focused ion beams and other imaging tools located at the Harvard Center for Nanoscale Systems (https://cns1.rc.fas.harvard.edu/).

More details on the position and the application process are available at https://www.elbaggarilab.com/opportunities/. Pre-submission inquiries via email are welcome.

Regards,

Ismail El Baggari PhD
Principal Investigator & Rowland Fellow
The Rowland Institute at Harvard
Cambridge, MA
www.elbaggarilab.com

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From: neumann-at-mpipz.mpg.de
Date: Fri, 20 Nov 2020 03:28:41 -0600
Subject: [Microscopy] TEM: plant peroxisomal marker for IGL

Contents Retrieved from Microscopy Listserver Archives
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Dear all,

I am looking for an antibody to be used as a peroxisomal marker at the
TEM level on sections of resin-embedded plant tissue, preferably a
rabbit polyclonal, but a monoclonal would also work.

Any recommendations, also on working dilutions, would be very much
appreciated!

Thanks a lot in advance, Ulla Neumann

Central Microscopy, MPI Plant Breeding Research, Cologne, Germany

==============================Original Headers==============================
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From: jacques.faerber-at-ipcms.unistra.fr
Date: Fri, 20 Nov 2020 15:34:27 -0600
Subject: [Microscopy] SEM first in lens SE detector ?

Contents Retrieved from Microscopy Listserver Archives
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Hi all

Could someone remind me when and by which manufacturer (on which
instrument) the first in lens SE detector was implemented on a SEM ? And
if you could point me on some papers ?

I know that in the deveoppement of the STEM by Akashi et al. on the JEOL
100 CX TEM (~1970 ?) they put a SE detector above the upper polepiece
(no room enough between the two polepieces) and got a "pure" SE signal.
But I don't remember when it was for the SEM and if Jeol did that. Or
was it ISI/ABT ?

Many thanks and best regards

Jacques

--

J. Faerber
IPCMS-DSI
Institut de Physique et Chimie des Matériaux de Strasbourg
Département Surfaces et Interfaces
23, rue de Loess ; BP43
67034 Strasbourg CEDEX 2
France

E-mail : Jacques.Faerber-at-ipcms.unistra.fr


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From: microscopy.listserver-at-gmail.com
Date: Sat, 21 Nov 2020 19:00:19 -0600
Subject: [Microscopy] viaWWW: JEOL 2010 built-in computer Program

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Email: hailstone-at-cis.rit.edu Name: Rich Hailstone

Title-Subject: [Filtered] JEOL 2010 built-in computer

Message: We recently had to shut down our JEOL 2010 for a repair. When we restarted the TEM, we
found the JEOL built-in computer had some issues. The computer had a program called "HTUP" which
allowed the user to program the HT to move up from one kV to another, over a prescribed time period
and in prescribed kV steps. Now the computer says "file not found" when we try to use that program.
Does anyone have such a program on their 2010 that they can share? Otherwise I'll have to figure out
the JEOL rudimentary programming language and re-create the program. Not looking forward to that!

Rich
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From: microscopy.listserver-at-gmail.com
Date: Sat, 21 Nov 2020 19:01:11 -0600
Subject: [Microscopy] viaWWW:Fixing a EM208S system

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Email: desertrat99-at-verizon.net Name: Eric Rosen

Title-Subject: [Filtered] Fixing a EM208S system.
Message: Does anyone know someone who can service a EM208S? I am having a problem with the High
Tension.
The system will all me to scope for a while. When unused we leave the vacuum system on all the
time. Then all of the sudden the HT will not energize when I push and hold the button.
To get it working again I have to shutdown the entire system and wait overnight. Power up the
scope and vacuum and the HT work again for a while.
Any suggestions?
I have not smelled anything burning or melting plastic on the circuit boards. I do not have enough
skill set to test out the HT rack boards.
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From: diller-at-stefan-diller.com
Date: Sun, 22 Nov 2020 03:33:11 -0600
Subject: [Microscopy] Re: viaWWW:Fixing a EM208S system

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Hello Eric,

I once had an EM208 my own but sold it some time later. Unfortunately I don`t have any manuals left to help you or somebody
electronically knowledgable to the exact point where to look...

...

Some questions:

- Did the HT voltage switch off on its own after some time when working with the scope? That would point more to a heat problem in
the elctronics... Or a faulty relay or missing 24 volts control voltage (but then the whole scope will shut down...)

- "HT voltage ON" state depends on a lot of closed "interlock" states from various (safety) circuit in the instrument, like
sufficient air pressure, cooling water, temperature of power regulators, vacuum pressure etc.

There might be something wrong or coming up wrong when using the scope for some time. Voltage at one of the power supplies might
just be right when electronics is cold and the be out of spec when warm...

To find out you have to provide more information how exactly and when the error occurs.

...

Did you change the high tension voltage at some point and does the problem also arise ? Lower HT voltage and lower magnification
mostly means lower power needed from the supplies and maybe - if this is a thermally induced behaviour - the drop-out will come
later. You have to check this first...


Best wishes,

Stefan


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Arndtstrasse 22
D - 97072 Wuerzburg Germany
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Websites:
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} Email: desertrat99-at-verizon.net Name: Eric Rosen
}
} Title-Subject: [Filtered] Fixing a EM208S system.
} Message: Does anyone know someone who can service a EM208S? I am having a problem with the High
} Tension.
} The system will all me to scope for a while. When unused we leave the vacuum system on all the
} time. Then all of the sudden the HT will not energize when I push and hold the button.
} To get it working again I have to shutdown the entire system and wait overnight. Power up the
} scope and vacuum and the HT work again for a while.
} Any suggestions?
} I have not smelled anything burning or melting plastic on the circuit boards. I do not have enough
} skill set to test out the HT rack boards.
} Login Host: 149.142.103.136
} Listserver Email Form V - 20120416
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From: clj46-at-drexel.edu
Date: Sun, 22 Nov 2020 08:03:37 -0600
Subject: [Microscopy] SEM Research Instrumentation Specialist Position at Drexel University

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

The Materials Characterization Core at Drexel University is hiring a Research Instrument Specialist to manage our scanning electron microscopes (2 SEMs and a FIBSEM). Complete information and a link to apply can be found on Drexel's Careers site:

https://careers.drexel.edu/en-us/job/495258/research-instrumentation-specialist

The Materials Characterization Core (MCC) is a centrally managed shared-instrumentation facility that provides Drexel University and external users access to advanced materials characterization instrumentation, training and expertise. The facility is centrally administered through the University's Office of Research and Innovation and has been providing research instrumentation access for over 14 years. The Research Instrumentation Specialist reports to the facility's full-time Director of Operations.

Essential Functions of the Research Instrumentation Specialist

* Provide outstanding service to MCC users through comprehensive training MCC SEMs, FIBSEM and sample preparation equipment. Applicant should be able to train a user who has no prior knowledge of the instruments
* Assist trained users to optimize the instrument and samples for their desired experiment
* Coordinate the maintenance of the instruments
* Provide expert consultation on experimental design and applications
* Manage users and track & confirm usage of instruments using iLab software
* Develop multimedia training materials for users
* Advise Director of Operations and others on instrument acquisitions and upgrades
* Other duties as assigned

Thanks,
Craig Johnson, Ph.D.
Director of Operations
TEM & FIBSEM Manager
Materials Characterization Core (MCC)
drexel.edu/core-facilities/facilities/material-characterization/



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From: microscopy.listserver-at-gmail.com
Date: Sun, 22 Nov 2020 08:09:35 -0600
Subject: [Microscopy] Fwd: Leica AFS electric scheme

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Denis Korneev {denis.korneev-at-monash.edu}


Dear colleagues,

I'm trying to fix one old Leica AFS (automatic freeze substitution) unit now.

Does anyone have the electric scheme for this old but still good machine?

Thank you in advance,
Denis
*---------------------------
*
*Denis Korneev, PhD *
Research Fellow
School of Biological Sciences
Monash University
25 Rainforest Walk, 3800 Clayton (Australia)

M:*+61 48 123 2155*_
_

Email: denis.korneev-at-monash.edu {mailto:denis.korneev-at-monash.edu}

Web: https://www.researchgate.net/profile/Denis_Korneev
{https://www.researchgate.net/profile/Denis_Korneev}


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From: microscopy.listserver-at-gmail.com
Date: Sun, 22 Nov 2020 08:11:16 -0600
Subject: [Microscopy] Fwd: viaWWW:how to crop image in Gatan Digital

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Ray Twesten {Ray.Twesten-at-ametek.com}

Ravi,
Select the region you wish to keep using the rectangular ROI tool (for GMS3 right-click on the
image to select the tool or for regular raster images this is the default tool so hold ctrl-key left
click and drag to select the ROI). Copy the region to the clip board (ctrl-c) and paste to a new
image (ctrl-alt-v) to create the cropped version.
The "paste new image" is a one of those legacy features that does not show up in the menus (I
know it really should). There is a list of these features in the help file which I have copied below.

Best regards,
Ray

Shortcuts
DigitalMicrograph provides many keyboard shortcuts. Most of those shortcuts are equivalent to menu
items and the shortcut can be read on the menu item. For example in the File menu, the Open... item
has "Ctrl+O" listed on it. This means that pressing the "O" key, while holding down the Control key
will be equivalent to choosing Open... from the File menu.
Some other shortcuts in DigitalMicrograph are not displayed on menu items:
Shift-Ctrl-W
Close all windows, but ask the user whether to save them, if needed.
Alt-Shift-Ctrl-W
Close all windows, including the Results window, and do not ask the user if data needs to be saved.
If you want to just close all the image windows, you can hold down the Alt, Ctrl and Shift keys and
then click in the close box of one of the image windows. In a similar way can you close all script
windows.
Alt-Ctrl-W
Close the foremost window without asking the user if the related data needs to be saved. You can
also hold down the Alt key, and then click in the close box of the image window you want to close.
Alt-Ctrl-F4
Close DigitalMicrograph without asking the user to save any data.
Alt-Ctrl-V
Paste image data in the paste buffer in a new image document window.
Alt-Ctrl-X
Cut the ROI from the image. With the normal Cut command on the File menu, or with Ctrl-X, you cut
the data in the ROI. When you hold down the Alt key as well, you actually cut the ROI itself, which
you can then paste into another document.
Alt-Ctrl-C
Copy the ROI from the image. With the normal Copy command on the File menu, or with Ctrl-C, you copy
the data in the ROI. When you hold down the Alt key as well, you actually copy the ROI itself, which
you can then paste into another document.
Alt-Ctrl-H
Show the options dialog for setting the number of channels in the Histogram and then display the
histogram. See "Using Histograms".
Alt-Ctrl-T
Show the options dialog for setting the number of channels in the Histogram and then display the
histogram for Thresholding. See "Thresholding".


Ray D. Twesten, Ph.D.
Product Manager - Analytical Instruments
Gatan, Inc.
Tel. +1 (925) 224-7392

This message and any attachments are solely for the use of intended recipients. They may contain
privileged and/or confidential information.

-----Original Message-----
X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Wednesday, November
18, 2020 2:55 PM
To: Ray Twesten {Ray.Twesten-at-ametek.com}

X-from: Tom Schamp {tom.schamp-at-protonmail.com}

Hi Ravi,

I think the easiest way is through scripting (ctrl + k). So if you are trying to crop image A, the
command to create a new image that is a cropped version of A where you have already defined a
rectangular ROI is "AA=A[]"

Note that you will not be transferring any tags or attributes to the new image.

Best regards,

Tom


Sent with ProtonMail Secure Email.

‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐
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From: neumann-at-mpipz.mpg.de
Date: Tue, 24 Nov 2020 01:41:43 -0600
Subject: [Microscopy] LM: antibodies to label plant peroxisomes by IFL/IC

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear all,

as my last email asking for anti-peroxisomal antibodies to be used for
IGL of resin-embedded plant tissues at the TEM level yielded zero
replies, I modify my request and switch to the LM level.

Can someone recommend an antibody to be used as a peroxisomal marker for
IFL/IC at the LM level on sections of resin-embedded plant tissue
(preferably a rabbit polyclonal, but a monoclonal would also work).

Any recommendations, also on working dilutions, would be very much
appreciated!

Thanks a lot in advance, Ulla Neumann

Central Microscopy, MPI Plant Breeding Research, Cologne, Germany




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From: nizets2-at-yahoo.com
Date: Thu, 26 Nov 2020 05:57:57 -0600
Subject: [Microscopy] methylene blue/Azur II staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Dear colleagues,

a skin (punch) biopsy was cut in half, one half was embedded in paraffin and H&E stained, the other half was epon-embedded and methylene blue/Azur II stained.
Some parts of the biopsy are made of freshly healed tissue and are therefore devoid of collagen fibers. This is very easy to see with H&E.
But what about methylene blue/azur II? Is there any chance that collagen is stained as well?
As a biologist I was trained to recognize healthy tissue but I have a hard tissue analyzing abnormal tissue patterns.
Can a pathologist help me here?

Best regards,
Stephane

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3, 52 -- From: Stephane Nizet {nizets2-at-yahoo.com}
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From: wij.muss-at-aon.at
Date: Thu, 26 Nov 2020 09:18:52 -0600
Subject: [Microscopy] Re: methylene blue/Azur II staining

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

-----Ursprngliche Nachricht-----
Von: MUSS Wolfgang Dr. phil./PhD (OR i.R, retired) [mailto:wij.muss-at-aon.at]
Gesendet: Donnerstag, 26. November 2020 16:26
An: 'nizets2-at-yahoo.com' {nizets2-at-yahoo.com}
Cc: 'Microscopy-at-Microscopy.com' {Microscopy-at-Microscopy.com} but
unfortunately as 'html'

So this time forwarded only to {Microscopy-at-Microscopy.com}

Conc.: Betreff: Re: [Microscopy] methylene blue/Azur II staining



A semithin resin section - depending on the former processing (starting with
fixation - ending with resin mixture and polymerization) - of a skin punch
biopsy containing collagen fibrils stained with Methylene blue / Azure II
alone (depending on the execution of a specific modification or use of
staining kit...so it would be not only interesting which method is used to
achieve prominent staining without overstaining) not necessarily might stain
('all') collagen fibrils / fibers.

In the case of using an 'AMbF' (Azure II-Methylene blue-basic Fuchsin)-stain
sequence (e.g. HUMPHREY and PITTMAN 1965, unmodified [depending on the resin
used] or modified by myself in the 1980ies and routinely used until 2015 for
diagnostic LM-TEM) I could nearly guarantee that you achieve positive red
staining for collagen fibrils/fibersas said depending on the previous
processing parameters.

Only as a notice added in brief: in the mid 1980ies there was a change in
the sources of EPON 812. At that time I noticed that following the
processing according to HUMPHREY and PITTMAN, as well as LACZK and LVAI
resulted in no more optimal staining for unknown reason. After a certain
modification (not published yet) I was able to get reasonable results again
(without overstaining).

But it might be that you already have seen some semithin section stainings
of this kind elsewhere [as such are displayed in Conference presentations I
uploaded them in the ResearchGate Database under
http://www.researchgate.net/profile/Wolfgang_MUSS ]

References:
HUMPHREY Ch. D., PITTMAN F.E. (1974) (Azur- II
-Methylenblau-basisches Fuchsin)
A simple Methyleneblue-Azure II Basic Fuchsin
Stain
for Epoxy-Embedded Tissue Sections
Stain Technology 49, 9-14 (1974)

or cf. also
LACZK Jen and LVAI Geza
A Simple Differential Staining Method for Semi-thin Sections
of Ossifying Cartilage and Bone Tissues Embedded
in Epoxy Resin
(Eine einfache Frbemethode zur Differenzierung des verkalkenden Knorpel.
und
Knochengewebes nach Einbettung in Epoxy[Epoxid]harz)
Mikroskopie (WIEN/Vienna), 31(1975) p. 1-4

Further information you might find in the MSA-List server archives of the
1980ies / 1990ies

Best regards,
W.H.M



MUSS Wolfgang Dr. phil. (PhD)
[OR i. R. / en retraite / retired]

Ignaz-Rieder-Kai 19/6
A-5020 SALZBURG
sterreich-AUSTRIA
Mobil-Tel.: 0043(0)676 5 369 456
E-mail: wij.muss-at-aon.at
E-Mail altern.: womuss-at-gmail.com

FRMS, Retired Member of MSA,
Member of BSC and other Societies

Scientific Profile at ResearchGate:
http://www.researchgate.net/profile/Wolfgang_MUSS
Former Head of Electron Microscopy Lab at Institute of Pathology
SALK-LKH / Salzburger Landeskliniken | General Hospital and
PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG


-----Ursprngliche Nachricht-----
Von: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com]
Gesendet: Donnerstag, 26. November 2020 12:59
An: wij.muss-at-aon.at
Betreff: [Microscopy] methylene blue/Azur II staining




----------------------------------------------------------------------------
The Microscopy ListServer -- CoSponsor: The Microscopy Society of America

Dear colleagues,

a skin (punch) biopsy was cut in half, one half was embedded in paraffin and
H&E stained, the other half was epon-embedded and methylene blue/Azur II
stained.
Some parts of the biopsy are made of freshly healed tissue and are therefore
devoid of collagen fibers. This is very easy to see with H&E.
But what about methylene blue/azur II? Is there any chance that collagen is
stained as well?
As a biologist I was trained to recognize healthy tissue but I have a hard
tissue analyzing abnormal tissue patterns.
Can a pathologist help me here?

Best regards,
Stephane

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From: vray-at-partbeamsystech.com
Date: Thu, 26 Nov 2020 14:54:48 -0600
Subject: [Microscopy] Raith "BB 8kV" Beam Blank Controller schematics

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

I am wondering if someone on the list may have schematics for "BB 8kV"
Beam Blank Controller made by Raith? I have a unit with a peculiar
failure of switching power supply board that generates 8.25kV output.
Reversing the design isn't a big deal, but takes time and working with
readily available schematics could be so much easier and quicker...

Thanks beforehand,
Valery
--
Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
www.partbeamsystech.com
www.fibsemproducts.com
www.freudlabs.com

"Only the Paranoid Survive" (A.Grove & SpaceX QA)

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From: peter.heimann-at-uni-bielefeld.de
Date: Thu, 26 Nov 2020 17:13:06 -0600
Subject: [Microscopy] Re: methylene blue/Azur II staining ( Peter Heimann )

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Hello Stephane,

collagen usually stains well in semithin sections with methylene
blue/Azur II aka Richardson Blue. In some cases it can become difficult
to discriminate collagen layers / fibers e.g. if tightly adjacent cells
are stained heavily or e.g. you won't be able to discriminate collagen
layers from myelin in myelinated nerves, or, another example, collagen
layers of arteries or arterioles usually stain very well with Richardson
Blue. Please contact me offline if you want to have some examplary
pictures...

regards,

Peter Heimann

(ex-Cell Biology; University of Bielefeld)

++++++++++++++++++++++++++++++++++++++++++++++++++++++++

Am 26.11.2020 um 13:01 schrieb nizets2-at-yahoo.com:
}
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} Dear colleagues,
} a skin (punch) biopsy was cut in half, one half was embedded in paraffin and H&E stained, the other half was epon-embedded and methylene blue/Azur II stained.
} Some parts of the biopsy are made of freshly healed tissue and are therefore devoid of collagen fibers. This is very easy to see with H&E.
} But what about methylene blue/azur II? Is there any chance that collagen is stained as well?
} As a biologist I was trained to recognize healthy tissue but I have a hard tissue analyzing abnormal tissue patterns.
} Can a pathologist help me here?
}
} Best regards,
} Stephane
}
} ==============================Original Headers==============================
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} 3, 52 -- Date: Thu, 26 Nov 2020 12:11:22 +0000 (UTC)
} 3, 52 -- From: Stephane Nizet {nizets2-at-yahoo.com}
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7, 22 -- Subject: Re: [Microscopy] methylene blue/Azur II staining ( Peter Heimann )
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From: vince.carlino-at-ibssgroup.com
Date: Mon, 30 Nov 2020 00:27:15 -0600
Subject: [Microscopy] Deposit thin layer of quartz/SiO2 on a QCTM crystal

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I am curious to learn if anyone on the list has a method to deposit a thin layer of quartz/SiO2 on a QCM (quartz balance crystal) on top of the gold or silver layer of the QCM crystal or a company that can do it?

Thanks beforehand,

Vincent Carlino
ibss Group, Inc.
www.ibssgroup.com
111 Anza Blvd. Suite 110
Burlingame, CA 94010
T: 650.513.1488
C: 415.900.7193





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7, 32 -- From: Vincent Carlino {vince.carlino-at-ibssgroup.com}
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7, 32 -- Subject: Deposit thin layer of quartz/SiO2 on a QCTM crystal
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From: vray-at-partbeamsystech.com
Date: Mon, 30 Nov 2020 09:24:29 -0600
Subject: [Microscopy] Re: Deposit thin layer of quartz/SiO2 on a QCTM crystal

Contents Retrieved from Microscopy Listserver Archives
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Hi Vince,

Either dielectric (optical) E-Beam evaporator or RF sputter coater would
deposit very thin film of SiO2. I am familiar with Denton equipment, but
I'm sure there are others. Many University-based semiconductor
facilities would have these tools, just Google.

Thicker layers may be easier to deposit via SOD (Spin-On Dielectric) or
SOG (Spin-On Glass) formulas made for semiconductor manufacturing. I've
used Honeywell, but others are without doubt are also available.

Valery Ray (also with REFINE Lab, UCONN)
==========================================
MEO Engineering Company, Inc. DBA "PBS&T"
290 Broadway, Suite 298, Methuen MA 01844
E-mail: vray-at-partbeamsystech.com
Phone: +1-978-305-0479
www.partbeamsystech.com
www.fibsemproducts.com
www.freudlabs.com

"Only the Paranoid Survive" (A.Grove & SpaceX QA)

On 11/30/2020 1:27 AM, vince.carlino-at-ibssgroup.com wrote:
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} I am curious to learn if anyone on the list has a method to deposit a thin layer of quartz/SiO2 on a QCM (quartz balance crystal) on top of the gold or silver layer of the QCM crystal or a company that can do it?
}
} Thanks beforehand,
}
} Vincent Carlino
} ibss Group, Inc.
} www.ibssgroup.com
} 111 Anza Blvd. Suite 110
} Burlingame, CA 94010
} T: 650.513.1488
} C: 415.900.7193
}
}
}
}
}
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6, 59 -- Subject: Re: [Microscopy] Deposit thin layer of quartz/SiO2 on a QCTM crystal
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From: crwinkler-at-ncsu.edu
Date: Tue, 1 Dec 2020 10:11:55 -0600
Subject: [Microscopy] X-ray microscopy postdoc opening at NCSU

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello all,

The Analytical Instrumentation Facility (AIF) at North Carolina State
University (NCSU) is seeking a talented and industrious
experimentalist to join our team as an X-ray Microscope (XRM)
Postdoctoral Researcher. Representative duties include training users
on operation of the X-ray microscope and CT instrument, performing
X-ray microscopy services using Zeiss Xradia 510 and the Bruker
SkyScan 1174 instruments, implementing at least 4 workshops and/or
user short courses each year, and staying abreast of software and
hardware developments by reading current literature and attending
relevant technical conferences.

For more details and to apply, please visit:
https://jobs.ncsu.edu/postings/137551

Thanks,
Chris

--
Transmission Electron Microscopy Lab Manager
Analytical Instrumentation Facility (AIF)
NC State University
https://www.aif.ncsu.edu/
Cell: 267-496-0587

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From: microscopy.listserver-at-gmail.com
Date: Thu, 3 Dec 2020 06:08:29 -0600
Subject: [Microscopy] viaWWW: Job opening, EM core facility at St. Jude Children's Research

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Email: cam.robinson-at-stjude.org Name: Cam Robinson

Title-Subject: [Filtered] Job opening, EM core facility at St. Jude Children's Research Hospital

Message: Hello all,

The EM Shared Resource at St. Jude Childrens Research Hospital is hiring at up to the Associate
Scientist level for an electron microscopist to join their team. Full details and a link to apply
can be found at:
https://careers-stjude.icims.com/jobs/6112/associate-scientist/job?mode=view&mobile=false&width=1180&height=500&bga=true&needsRedirect=false&jan1offset=-360&jun1offset=-300

The Cell and Tissue Imaging Center at St. Jude Childrens Research Hospital is looking for a
talented and driven electron microscopist to join the Center at the Associate Scientist level. The
electron microscopy facility houses a Thermo Fisher Scientific F20, a Helios FIB/SEM and a Teneo
VolumeScope SEM as well as a variety of equipment for sample preparation including Leica tissue
processors and ultramicrotomes, equipment for low temperature processing and multiple vacuum
evaporators. The facility is funded by the Hospital and is utilized by faculty from across the
research departments, providing world class imaging to the investigators at St. Jude. Staff members
in the Center collaborate with investigators on a variety of research projects from standard tissue
pathologies through three-dimensional imaging projects by electron tomography or volume SEM and have
the opportunity to participate in development of techniques and protocols to drive St. Judes
research forward. This position requires experience in biological electron microscopy. Skill sets
in volume SEM (scanning block face SEM, array tomography, and FIB/SEM) and/or high pressure freezing
and freeze substitution are highly desirable.
Thanks, Cam

Camenzind G Robinson, PhD
Director, Electron Microscopy Shared Resource
Cell and Tissue Imaging Center
St. Jude Childrens Research Hospital
262 Danny Thomas Place, Mail Stop 312
Memphis, TN 38105-3678

https://www.stjude.org/research/shared-resources/cell-and-tissue-imaging-center/electron-microscopy-core-facility.html

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From: microscopy.listserver-at-gmail.com
Date: Thu, 3 Dec 2020 06:10:00 -0600
Subject: [Microscopy] viaWWW: Stanford EM-X Symposium this Monday Dec. 7th (Cryo-EM & in

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X-from: rvila-at-stanford.edu

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Email: rvila-at-stanford.edu Name: Rafael A. Vila

Title-Subject: [Filtered] Stanford EM-X Symposium this Monday Dec. 7th (Cryo-EM & in situ-EM)

Message: Rafael Vil {rvila-at-stanford.edu}

Join us Monday December 7th at 8:00 AM (PST) for the second symposium in our new EM-X series.


We are excited to have two incredible speakers this month:


Dr. Richard Henderson (2017 Nobel Prize winner in Chemistry) will talk about The path towards
affordable cryo-EM.


Prof. Frances Ross (Prof. at MIT) will talk about "Microscopy in motion: understanding crystal
growth through in situ electron microscopy."


Please visit our website (emx.stanford.edu) for more information (symposium registration, previous
talks, upcoming events, etc.)


For a direct link to register for the symposium please visit:
https://stanford.zoom.us/webinar/register/WN_58NcmULsRciJeTAvWd7dnA



Best Regards,

Rafael A. Vila

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From: microscopy.listserver-at-gmail.com
Date: Thu, 3 Dec 2020 06:11:06 -0600
Subject: [Microscopy] Fwd: thanks for the O-rings

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X-from: Frank Karl {frank_karl-at-ardl.com}

I want to thank everyone who helped out and pointed me at O-rings for my TEM sample rod.

It's iced to know the microscopy community is so strong.



Stay calm...Be brave....watch for signs

Frank Karl
Microscopist
Akron Rubber Development Laboratory
2887 Gilchrist Road
Akron, Ohio 44305


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From: microscopy.listserver-at-gmail.com
Date: Fri, 4 Dec 2020 06:38:57 -0600
Subject: [Microscopy] viaWWW: Holder for t-EBSD/TKD

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X-from: talukder.m.alam-at-gmail.com

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Email: talukder.m.alam-at-gmail.com Name: Talukder Alam

Title-Subject: [Filtered] Holder for t-EBSD/TKD

Message: Dear Microscope community,

I am looking for suggestions for a suitable TKD holder for a Quanta 650 system. If this forum is not
the ideal platform I am available to communicate off-track.

I would also like to know about your experience with the data analysis regards the TKD. Was the
existing EBSD software able to do the analysis?

Appreciate all the help!
Regards,
Talukder Alam


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From: microscopy.listserver-at-gmail.com
Date: Sat, 5 Dec 2020 05:27:00 -0600
Subject: [Microscopy] Fwd: viaWWW: Holder for t-EBSD/TKD

Contents Retrieved from Microscopy Listserver Archives
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X-from: Greg Baty {gbaty-at-pdx.edu}

You should check with your EBSD provider to make sure you have all of
the needed software. My lab has used Oxford Aztec since 2.0. I believe
t EBSD acquisition since Aztec 2.0 and Chanel 5 for analysis is
possible. These days we use Aztec crystal for processing.

As for a sample holder, you may either make one or purchase a readily
available holder. I do not have a recommendation for a quanta. It will
depend on how much lens to stage clearance you have. One of my lab
users has the Bruker t EBSD holder. It is a really nice unit but was a
tight fit in the FEI xl30 Sirion. No problem to fit in our zeiss
sigma.

Greg Baty
Manager, CEMN
Portland State University

Sent from my iPhone

} On Dec 4, 2020, at 4:42 AM, microscopy.listserver-at-gmail.com wrote:
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} Title-Subject: [Filtered] Holder for t-EBSD/TKD
}
} Message: Dear Microscope community,
}
} I am looking for suggestions for a suitable TKD holder for a Quanta 650 system. If this forum is not
} the ideal platform I am available to communicate off-track.
}
} I would also like to know about your experience with the data analysis regards the TKD. Was the
} existing EBSD software able to do the analysis?
}
} Appreciate all the help!
} Regards,
} Talukder Alam
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From: microscopy.listserver-at-gmail.com
Date: Sat, 5 Dec 2020 05:28:10 -0600
Subject: [Microscopy] Fwd: viaWWW: Holder for t-EBSD/TKD

Contents Retrieved from Microscopy Listserver Archives
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-------- Forwarded Message --------


We made our own holder.

A composite from a standard TEM grid holder mounted on a Jeol TEM grid holder cut in half.


https://www.sciencedirect.com/science/article/abs/pii/S0304399115300619

https://www.google.com/search?source=univ&tbm=isch&q=transmissie+ebsd+sample+holder&sa=X&ved=2ahUKEwjpptOLuLTtAhXiJMUKHZxdA18QjJkEegQIBRAB&biw=1194&bih=809

https://www.researchgate.net/figure/The-experimental-t-EBSD-set-up-in-the-Philips-FEI-FEG-XL30-ESEM-The-t-EBSD-holder-2_fig3_283562763


Good luck building your own :-)

Gert

On 04/12/2020 13:42, microscopy.listserver-at-gmail.com wrote:




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} Email:talukder.m.alam-at-gmail.com Name: Talukder Alam
}
} Title-Subject: [Filtered] Holder for t-EBSD/TKD
}
} Message: Dear Microscope community,
}
} I am looking for suggestions for a suitable TKD holder for a Quanta 650 system. If this forum is not
} the ideal platform I am available to communicate off-track.
}
} I would also like to know about your experience with the data analysis regards the TKD. Was the
} existing EBSD software able to do the analysis?
}
} Appreciate all the help!
} Regards,
} Talukder Alam
}
}
} Login Host: 128.46.207.92
} Listserver Email Form V - 20120416
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--
--
With kind regards,
Gert ten Brink
Dept. Nanostructured Materials and Interfaces
Zernike Institute for Advanced Materials
University of Groningen
Nijenborgh 4
9747 AG Groningen
The Netherlands
Room 5113-213
phone: +31(0)503634889; fax:+31(0)503634881
e-mail:g.h.ten.brink-at-rug.nl;
http://www.rug.nl/research/nanostructured-materials-interfaces/


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From: microscopy.listserver-at-gmail.com
Date: Sat, 5 Dec 2020 05:34:47 -0600
Subject: [Microscopy] viaWWW: Undergraduate Experience Webinar and Scholarship Information

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: anpenn-at-ncsu.edu

This Question/Comment was submitted to the Microscopy Listserver
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Email: anpenn-at-ncsu.edu Name: Aubrey Penn

Title-Subject: [Filtered] Undergraduate Experience Webinar and Scholarship Information Session

Message: Dear Microscopist,
The Microscopy Society of America Student Council (StC) will be hosting the Undergraduate Experience
webinar on December 10 for undergraduate students to learn about navigating undergraduate research
and gather information about the MSA Undergraduate Scholarship
(https://www.microscopy.org/awards/scholarship.cfm). Our invited panelists include previous
scholarship winners and an experienced PI to undergraduate researchers.
Participants can register at the following link to receive reminders and submit questions in advance
of the webinar (https://forms.gle/wpSk3CuKbf2gQg739), and join via Zoom on Thursday, December 10 at
12 PM ET. Participants will need to register to join us on Zoom and have the opportunity to ask
questions during the panel discussion. The webinar will also be live streamed on our Youtube page
(https://www.youtube.com/channel/UC091t-wxZm0-4neu1jU7Log/).

StC is excited to announce that any MSA member who attends all webinars in this years Webinar
Series will be entered in a drawing to win a StC merchandise package including a T-shirt, mug and more!

Our panelists are:
Will Bowman, PhD
Assistant Professor, UC Irvine

Noah Schnitzer
Graduate Student, Cornell University
MSA Undergraduate Scholarship Awardee

Jackson Spurling
MSA Student Council Treasurer Undergraduate Researcher at UT Knoxville


Any questions can be directed to studentcouncil-at-microscopy.org.
Sincerely, Aubrey Penn
President Elect, MSA StC


Login Host: 107.13.169.11
Listserver Email Form V - 20120416
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From: xin-at-magnet.fsu.edu
Date: Mon, 7 Dec 2020 14:31:50 -0600
Subject: [Microscopy] inquiry on standard practice on co-authorship request

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi, All,

I would appreciate if you can give me some input on standard practice
concerning co-authorship on TEM data collection by the staff.
Is it appropriate for the staff to ask for co-authorship if the TEM staff
collects the TEM data and the TEM images are used in the publication? The
TEM user only pays the machine time, not the staff labor time. There is
no data analysis involved.
Or in this case, the user should just acknowledge the staff with funding
information in the acknowledgement?

Thanks very much
Yan Xin
FSU



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From: oystein.prytz-at-fys.uio.no
Date: Tue, 8 Dec 2020 01:39:50 -0600
Subject: [Microscopy] Re: inquiry on standard practice on co-authorship

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello!

This is an interesting question where I feel that there is a lot of
confusion. In an academic setting the question of funding, or who pays
for what, isn't really relevant. The only question that needs to be
answered is if a person has contributed "enough" to the science be
identified as an author. In our lab we try to follow the Vancouver
recommendations:

http://www.icmje.org/icmje-recommendations.pdf

which give the following guidelines:

"2. Who Is an Author?
The ICMJE recommends that authorship be based on the following 4
criteria:
1. _Substantial contributions to_ the conception or design of the
work; _or the acquisition, analysis, or interpretation of data for
the work_; AND
2. Drafting the work or revising it critically for important
intellectual content; AND
3. Final approval of the version to be published; AND
4. Agreement to be accountable for all aspects of the work in
ensuring that questions related to the accuracy or integrity of any
part of the work are appropriately investigated and resolved.

[...]

The criteria are not intended for use as a means to disqualify
colleagues from authorship who otherwise meet authorship criteria by
denying them the opportunity to meet criterion #s 2 or 3.Therefore,
all individuals who meet the first criterion should have the
opportunity to participate in the review,drafting, and final
approval of the manuscript."

For the case that you are describing I believe the underlined part is
the most relevant. As you see, any person who has given substantial
contribution to acquisition of data "for the work" should be recognized
as an author. Any data that is included in the manuscript would have to
fall under the heading of "for the work", and a person who in actual
fact was alone responsible for acquiring that data would necessarily
have to be recognized as giving a "substantial contribution" to the
acquisition. So my conclusion would be that yes, it is usually
appropriate for staff to be included in the list of authors.

On a more general note, I would like to stress that in my opinion
anything beyond the very simplest experiments in TEM require a level of
scientific understanding that goes beyond a "technical" task. Decisions
must be made on sample preparation and design, finding appropriate
projections for diffraction and imaging, in STEM deciding on detectors
and illumination conditions to use, in EELS deciding on which edges to
select to find the information needed, in EDS understanding if
absorption and fluorescence is an issue, and multiple other small and
large decisions taken on the fly during the experiment. Many of these
are things I expect my students to describe and discuss in their thesis,
and if they don't I would conclude that they have not done a proper
scientific job. Personally I would have been hesitant to submit a paper
without including the person actually doing this job; who is then
ensuring that what we write is correct?

Finally, I have never understood the impulse that some people have to
limit the author list as much as possible. Of course, we shouldn't
include people improperly, but neither should we look for ways of
preventing people from becoming authors. That would very easily create a
culture where people are guarded and careful about where they help out,
and may even foster an atmosphere of mistrust and feelings of being
underappreciated. Personally I would much rather err on the side of
being too generous, than risk leaving out people who in reality should
have been included. If people generally feel secure in the knowledge
that their contributions will be acknowledged properly, they will be
more inclined to help out, and may in time also more easily feel that
they can forgo co-authorships for what they consider "trivial" tasks.
This makes for a more healthy scientific environment.

Best regards
Øystein

-
Øystein Prytz, PhD |
Professor of Physics | Mobile: +47 93201512
University of Oslo | Office: +47 22840684
oystein.prytz-at-fys.uio.no | http://folk.uio.no/oysteinp/group








On 07.12.2020 21:35, xin-at-magnet.fsu.edu wrote:
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} Hi, All,
}
} I would appreciate if you can give me some input on standard practice
} concerning co-authorship on TEM data collection by the staff.
} Is it appropriate for the staff to ask for co-authorship if the TEM staff
} collects the TEM data and the TEM images are used in the publication? The
} TEM user only pays the machine time, not the staff labor time. There is
} no data analysis involved.
} Or in this case, the user should just acknowledge the staff with funding
} information in the acknowledgement?
}
} Thanks very much
} Yan Xin
} FSU
}
}
}
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} 5, 110 -- Subject: inquiry on standard practice on co-authorship request
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}

--
Øystein Prytz, PhD |
Professor of Physics | Mobile: +47 93201512
University of Oslo | Office: +47 22840684
oystein.prytz-at-fys.uio.no | http://folk.uio.no/oysteinp/group




==============================Original Headers==============================
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24, 32 -- Subject: Re: [Microscopy] inquiry on standard practice on co-authorship
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==============================End of - Headers==============================




From: greggps-at-umich.edu
Date: Tue, 8 Dec 2020 14:29:41 -0600
Subject: [Microscopy] Fwd: Re: inquiry on standard practice on co-authorship

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

The subjective manner of choosing authorship recognition has always
been too far open to interpretation and bias, in my opinion.

In my Lab Manager job for a university decades ago, I collected all of
the data, from live animal sedation through perfusion, fixation,
staining, blocking and embedding, sectioning, UA-LC staining, imaging,
darkroom work, the Methods section, and all plates and posters labeled
and assembled (remember dry mount?). I didn't even receive
acknowledgement, since "I was being paid to do that" (as a
technician). By that logic, he's paid to be a Principal Investigator,
but I do understand that he does mentor the graduate student author
and assists with editing. To this day, I believe he wanted to claim
only academic creative contributions (versus technical) as
justification for authorship. He also did not include me when I
performed immunohistochemistry and very skill-intensive serial
sectioning montage posters. Personally, I believe he thinks it adds
more prestige to have only one or two authors on his papers.

In industry, we often had several co-authors, and our department head
included himself as last author for no other reason than he had to
read and approve the final result. This was company policy, and
therefore his job. He has over 700 papers to his name. Isn't that
prestigious? I recently read an official document justifying my label
of that behavior as completely inappropriate. Who was going to stop
him, though? He can kill the entire paper with a word.

I'm glad to see more precise definitions for authorship being
published, although most principal investigators will go with policies
based on their own opinion.

If it sounds like a gripe, it's because I've experienced both sides of
the coin. One or two authors as one standard and seven to twelve as
another standard. I think reasonable acknowledgment without bloat is
the way to go.

My opinion, for what it's worth.
~Gregg

Gregg Sobocinski
Microscope Imaging Suite, Managing Director
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA


On Tue, Dec 8, 2020 at 2:59 AM {oystein.prytz-at-fys.uio.no} wrote:
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello!
}
} This is an interesting question where I feel that there is a lot of
} confusion. In an academic setting the question of funding, or who pays
} for what, isn't really relevant. The only question that needs to be
} answered is if a person has contributed "enough" to the science be
} identified as an author. In our lab we try to follow the Vancouver
} recommendations:
}
} http://www.icmje.org/icmje-recommendations.pdf
}
} which give the following guidelines:
}
} "2. Who Is an Author?
} The ICMJE recommends that authorship be based on the following 4
} criteria:
} 1. _Substantial contributions to_ the conception or design of the
} work; _or the acquisition, analysis, or interpretation of data for
} the work_; AND
} 2. Drafting the work or revising it critically for important
} intellectual content; AND
} 3. Final approval of the version to be published; AND
} 4. Agreement to be accountable for all aspects of the work in
} ensuring that questions related to the accuracy or integrity of any
} part of the work are appropriately investigated and resolved.
}
} [...]
}
} The criteria are not intended for use as a means to disqualify
} colleagues from authorship who otherwise meet authorship criteria by
} denying them the opportunity to meet criterion #s 2 or 3.Therefore,
} all individuals who meet the first criterion should have the
} opportunity to participate in the review,drafting, and final
} approval of the manuscript."
}
} For the case that you are describing I believe the underlined part is
} the most relevant. As you see, any person who has given substantial
} contribution to acquisition of data "for the work" should be recognized
} as an author. Any data that is included in the manuscript would have to
} fall under the heading of "for the work", and a person who in actual
} fact was alone responsible for acquiring that data would necessarily
} have to be recognized as giving a "substantial contribution" to the
} acquisition. So my conclusion would be that yes, it is usually
} appropriate for staff to be included in the list of authors.
}
} On a more general note, I would like to stress that in my opinion
} anything beyond the very simplest experiments in TEM require a level of
} scientific understanding that goes beyond a "technical" task. Decisions
} must be made on sample preparation and design, finding appropriate
} projections for diffraction and imaging, in STEM deciding on detectors
} and illumination conditions to use, in EELS deciding on which edges to
} select to find the information needed, in EDS understanding if
} absorption and fluorescence is an issue, and multiple other small and
} large decisions taken on the fly during the experiment. Many of these
} are things I expect my students to describe and discuss in their thesis,
} and if they don't I would conclude that they have not done a proper
} scientific job. Personally I would have been hesitant to submit a paper
} without including the person actually doing this job; who is then
} ensuring that what we write is correct?
}
} Finally, I have never understood the impulse that some people have to
} limit the author list as much as possible. Of course, we shouldn't
} include people improperly, but neither should we look for ways of
} preventing people from becoming authors. That would very easily create a
} culture where people are guarded and careful about where they help out,
} and may even foster an atmosphere of mistrust and feelings of being
} underappreciated. Personally I would much rather err on the side of
} being too generous, than risk leaving out people who in reality should
} have been included. If people generally feel secure in the knowledge
} that their contributions will be acknowledged properly, they will be
} more inclined to help out, and may in time also more easily feel that
} they can forgo co-authorships for what they consider "trivial" tasks.
} This makes for a more healthy scientific environment.
}
} Best regards
} Øystein
}
} -
} Øystein Prytz, PhD |
} Professor of Physics | Mobile: +47 93201512
} University of Oslo | Office: +47 22840684
} oystein.prytz-at-fys.uio.no | http://folk.uio.no/oysteinp/group
}
}
} On 07.12.2020 21:35, xin-at-magnet.fsu.edu wrote:
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hi, All,
} }
} } I would appreciate if you can give me some input on standard practice
} } concerning co-authorship on TEM data collection by the staff.
} } Is it appropriate for the staff to ask for co-authorship if the TEM staff
} } collects the TEM data and the TEM images are used in the publication? The
} } TEM user only pays the machine time, not the staff labor time. There is
} } no data analysis involved.
} } Or in this case, the user should just acknowledge the staff with funding
} } information in the acknowledgement?
} }
} } Thanks very much
} } Yan Xin
} } FSU
} }
} } =====End of - Headers==============================


==============================Original Headers==============================
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10, 44 -- From: Gregg Sobocinski {greggps-at-umich.edu}
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10, 44 -- Subject: Fwd: [Microscopy] Re: inquiry on standard practice on co-authorship
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==============================End of - Headers==============================




From: oystein.prytz-at-fys.uio.no
Date: Wed, 9 Dec 2020 02:43:58 -0600
Subject: [Microscopy] Re: Fwd: Re: inquiry on standard practice on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gregg

I think that your experience unfortunately is quite common, and in my
opinion that way of doing it is inappropriate. A mention in the
acknowledgements should be the very minimum for what you describe.
Regarding who-gets-paid-for-what you are entirely on point: we are all
doing the job we are being paid for, so what?

I should hasten to say that in my first email I was a little imprecise:
fulfilling the first of the Vancouver criteria is not _alone_ enough to
qualify as a co-author, but qualifies to be invited to contribute on the
second (and third) criterion, and thereby further qualify as a co-author.

On the point of department heads or lab-leaders being co-authors as a
matter of policy: In my opinion this is not an acceptable way of doing
it. You might very well have a policy that the department head should
get the opportunity to be involved in such a way it qualifies a
co-author, but it is _that actual involvement_ that qualifies him/her as
a co-author, not the policy! That person should still fulfill the same
authorship criteria as everyone else (e.g. the Vancouver
recommendations). Whether or not he/she actually does fulfill those
criteria has to be evaluated in each specific case.

I don't have too much experience with such policies, I don't think they
are too common in Norway (or the Nordic countries in general). However,
as a student I have experienced it in some international collaborations.
In those cases I think it turned out to improve the final papers quite a
bit. Perhaps I was lucky, but what the policy in those cases did was to
secure input from very experienced scientists to my work. If implemented
properly, such policies could also be of benefit to junior researchers.

Best regards
Øystein



On 08.12.2020 21:34, greggps-at-umich.edu wrote:
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} The subjective manner of choosing authorship recognition has always
} been too far open to interpretation and bias, in my opinion.
}
} In my Lab Manager job for a university decades ago, I collected all of
} the data, from live animal sedation through perfusion, fixation,
} staining, blocking and embedding, sectioning, UA-LC staining, imaging,
} darkroom work, the Methods section, and all plates and posters labeled
} and assembled (remember dry mount?). I didn't even receive
} acknowledgement, since "I was being paid to do that" (as a
} technician). By that logic, he's paid to be a Principal Investigator,
} but I do understand that he does mentor the graduate student author
} and assists with editing. To this day, I believe he wanted to claim
} only academic creative contributions (versus technical) as
} justification for authorship. He also did not include me when I
} performed immunohistochemistry and very skill-intensive serial
} sectioning montage posters. Personally, I believe he thinks it adds
} more prestige to have only one or two authors on his papers.
}
} In industry, we often had several co-authors, and our department head
} included himself as last author for no other reason than he had to
} read and approve the final result. This was company policy, and
} therefore his job. He has over 700 papers to his name. Isn't that
} prestigious? I recently read an official document justifying my label
} of that behavior as completely inappropriate. Who was going to stop
} him, though? He can kill the entire paper with a word.
}
} I'm glad to see more precise definitions for authorship being
} published, although most principal investigators will go with policies
} based on their own opinion.
}
} If it sounds like a gripe, it's because I've experienced both sides of
} the coin. One or two authors as one standard and seven to twelve as
} another standard. I think reasonable acknowledgment without bloat is
} the way to go.
}
} My opinion, for what it's worth.
} ~Gregg
}
} Gregg Sobocinski
} Microscope Imaging Suite, Managing Director
} University of Michigan, MCDB Dept.
} Ann Arbor, Michigan
} USA
}
}
} On Tue, Dec 8, 2020 at 2:59 AM {oystein.prytz-at-fys.uio.no} wrote:
} }
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hello!
} }
} } This is an interesting question where I feel that there is a lot of
} } confusion. In an academic setting the question of funding, or who pays
} } for what, isn't really relevant. The only question that needs to be
} } answered is if a person has contributed "enough" to the science be
} } identified as an author. In our lab we try to follow the Vancouver
} } recommendations:
} }
} } http://www.icmje.org/icmje-recommendations.pdf
} }
} } which give the following guidelines:
} }
} } "2. Who Is an Author?
} } The ICMJE recommends that authorship be based on the following 4
} } criteria:
} } 1. _Substantial contributions to_ the conception or design of the
} } work; _or the acquisition, analysis, or interpretation of data for
} } the work_; AND
} } 2. Drafting the work or revising it critically for important
} } intellectual content; AND
} } 3. Final approval of the version to be published; AND
} } 4. Agreement to be accountable for all aspects of the work in
} } ensuring that questions related to the accuracy or integrity of any
} } part of the work are appropriately investigated and resolved.
} }
} } [...]
} }
} } The criteria are not intended for use as a means to disqualify
} } colleagues from authorship who otherwise meet authorship criteria by
} } denying them the opportunity to meet criterion #s 2 or 3.Therefore,
} } all individuals who meet the first criterion should have the
} } opportunity to participate in the review,drafting, and final
} } approval of the manuscript."
} }
} } For the case that you are describing I believe the underlined part is
} } the most relevant. As you see, any person who has given substantial
} } contribution to acquisition of data "for the work" should be recognized
} } as an author. Any data that is included in the manuscript would have to
} } fall under the heading of "for the work", and a person who in actual
} } fact was alone responsible for acquiring that data would necessarily
} } have to be recognized as giving a "substantial contribution" to the
} } acquisition. So my conclusion would be that yes, it is usually
} } appropriate for staff to be included in the list of authors.
} }
} } On a more general note, I would like to stress that in my opinion
} } anything beyond the very simplest experiments in TEM require a level of
} } scientific understanding that goes beyond a "technical" task. Decisions
} } must be made on sample preparation and design, finding appropriate
} } projections for diffraction and imaging, in STEM deciding on detectors
} } and illumination conditions to use, in EELS deciding on which edges to
} } select to find the information needed, in EDS understanding if
} } absorption and fluorescence is an issue, and multiple other small and
} } large decisions taken on the fly during the experiment. Many of these
} } are things I expect my students to describe and discuss in their thesis,
} } and if they don't I would conclude that they have not done a proper
} } scientific job. Personally I would have been hesitant to submit a paper
} } without including the person actually doing this job; who is then
} } ensuring that what we write is correct?
} }
} } Finally, I have never understood the impulse that some people have to
} } limit the author list as much as possible. Of course, we shouldn't
} } include people improperly, but neither should we look for ways of
} } preventing people from becoming authors. That would very easily create a
} } culture where people are guarded and careful about where they help out,
} } and may even foster an atmosphere of mistrust and feelings of being
} } underappreciated. Personally I would much rather err on the side of
} } being too generous, than risk leaving out people who in reality should
} } have been included. If people generally feel secure in the knowledge
} } that their contributions will be acknowledged properly, they will be
} } more inclined to help out, and may in time also more easily feel that
} } they can forgo co-authorships for what they consider "trivial" tasks.
} } This makes for a more healthy scientific environment.
} }
} } Best regards
} } Øystein
} }
} } -
} } Øystein Prytz, PhD |
} } Professor of Physics | Mobile: +47 93201512
} } University of Oslo | Office: +47 22840684
} } oystein.prytz-at-fys.uio.no | http://folk.uio.no/oysteinp/group
} }
} }
} } On 07.12.2020 21:35, xin-at-magnet.fsu.edu wrote:
} } }
} } }
} } }
} } } ----------------------------------------------------------------------------
} } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
} } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } } ----------------------------------------------------------------------------
} } }
} } } Hi, All,
} } }
} } } I would appreciate if you can give me some input on standard practice
} } } concerning co-authorship on TEM data collection by the staff.
} } } Is it appropriate for the staff to ask for co-authorship if the TEM staff
} } } collects the TEM data and the TEM images are used in the publication? The
} } } TEM user only pays the machine time, not the staff labor time. There is
} } } no data analysis involved.
} } } Or in this case, the user should just acknowledge the staff with funding
} } } information in the acknowledgement?
} } }
} } } Thanks very much
} } } Yan Xin
} } } FSU
} } }
} } } =====End of - Headers==============================
}
}
} ==============================Original Headers==============================
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--
Øystein Prytz, PhD |
Professor of Physics | Mobile: +47 93201512
University of Oslo | Office: +47 22840684
oystein.prytz-at-fys.uio.no | http://folk.uio.no/oysteinp/group




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13, 32 -- Subject: Re: [Microscopy] Fwd: Re: inquiry on standard practice on
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From: nizets2-at-yahoo.com
Date: Thu, 10 Dec 2020 02:46:24 -0600
Subject: [Microscopy] Re: Fwd: Re: inquiry on standard practice on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hi Gregg,


you make a very good point.
As a PhD student, my mentor even published my results without my name! And yes he can do it, he is all-powerful and you as a student simply have no right, the university is not a democracy. 
The same person also never included his technical assistant in his publication, although he couldn't publish anything without her, she was a very skillful technician. Another research group next door included all personel in all publication, independently of who did the real work (which may be questionable too but it doesn't hurt anybody, does it?).

I completely share you opinion: everyone involved in the paper should be mentionned. 
But scientists are human beings like others, with their ego problems and the complexity of human relationships as we know them.

Kind regards,
Stephane





On Tuesday, December 8, 2020, 09:49:17 PM GMT+1, greggps-at-umich.edu {greggps-at-umich.edu} wrote:








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The subjective manner of choosing authorship recognition has always
been too far open to interpretation and bias, in my opinion.

In my Lab Manager job for a university decades ago, I collected all of
the data, from live animal sedation through perfusion, fixation,
staining, blocking and embedding, sectioning, UA-LC staining, imaging,
darkroom work, the Methods section, and all plates and posters labeled
and assembled (remember dry mount?). I didn't even receive
acknowledgement, since "I was being paid to do that" (as a
technician). By that logic, he's paid to be a Principal Investigator,
but I do understand that he does mentor the graduate student author
and assists with editing. To this day, I believe he wanted to claim
only academic creative contributions (versus technical) as
justification for authorship. He also did not include me when I
performed immunohistochemistry and very skill-intensive serial
sectioning montage posters. Personally, I believe he thinks it adds
more prestige to have only one or two authors on his papers.

In industry, we often had several co-authors, and our department head
included himself as last author for no other reason than he had to
read and approve the final result. This was company policy, and
therefore his job. He has over 700 papers to his name. Isn't that
prestigious? I recently read an official document justifying my label
of that behavior as completely inappropriate. Who was going to stop
him, though? He can kill the entire paper with a word.

I'm glad to see more precise definitions for authorship being
published, although most principal investigators will go with policies
based on their own opinion.

If it sounds like a gripe, it's because I've experienced both sides of
the coin. One or two authors as one standard and seven to twelve as
another standard. I think reasonable acknowledgment without bloat is
the way to go.

My opinion, for what it's worth.
~Gregg

Gregg Sobocinski
Microscope Imaging Suite, Managing Director
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA


On Tue, Dec 8, 2020 at 2:59 AM {oystein.prytz-at-fys.uio.no} wrote:
}
}
}
}
} ----------------------------------------------------------------------------
} The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
} To  Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver
} On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} ----------------------------------------------------------------------------
}
} Hello!
}
} This is an interesting question where I feel that there is a lot of
} confusion. In an academic setting the question of funding, or who pays
} for what, isn't really relevant. The only question that needs to be
} answered is if a person has contributed "enough" to the science be
} identified as an author. In our lab we try to follow the Vancouver
} recommendations:
}
} http://www.icmje.org/icmje-recommendations.pdf
}
} which give the following guidelines:
}
} "2. Who Is an Author?
} The ICMJE recommends that authorship be based on the following 4
} criteria:
} 1. _Substantial contributions to_ the conception or design of the
} work; _or the acquisition, analysis, or interpretation of data for
} the work_; AND
} 2. Drafting the work or revising it critically for important
} intellectual content; AND
} 3. Final approval of the version to be published; AND
} 4. Agreement to be accountable for all aspects of the work in
} ensuring that questions related to the accuracy or integrity of any
} part of the work are appropriately investigated and resolved.
}
} [...]
}
} The criteria are not intended for use as a means to disqualify
} colleagues from authorship who otherwise meet authorship criteria by
} denying them the opportunity to meet criterion #s 2 or 3.Therefore,
} all individuals who meet the first criterion should have the
} opportunity to participate in the review,drafting, and final
} approval of the manuscript."
}
} For the case that you are describing I believe the underlined part is
} the most relevant. As you see, any person who has given substantial
} contribution to acquisition of data "for the work" should be recognized
} as an author. Any data that is included in the manuscript would have to
} fall under the heading of "for the work", and a person who in actual
} fact was alone responsible for acquiring that data would necessarily
} have to be recognized as giving a "substantial contribution" to the
} acquisition. So my conclusion would be that yes, it is usually
} appropriate for staff to be included in the list of authors.
}
} On a more general note, I would like to stress that in my opinion
} anything beyond the very simplest experiments in TEM require a level of
} scientific understanding that goes beyond a "technical" task. Decisions
} must be made on sample preparation and design, finding appropriate
} projections for diffraction and imaging, in STEM deciding on detectors
} and illumination conditions to use, in EELS deciding on which edges to
} select to find the information needed, in EDS understanding if
} absorption and fluorescence is an issue, and multiple other small and
} large decisions taken on the fly during the experiment. Many of these
} are things I expect my students to describe and discuss in their thesis,
} and if they don't I would conclude that they have not done a proper
} scientific job. Personally I would have been hesitant to submit a paper
} without including the person actually doing this job; who is then
} ensuring that what we write is correct?
}
} Finally, I have never understood the impulse that some people have to
} limit the author list as much as possible. Of course, we shouldn't
} include people improperly, but neither should we look for ways of
} preventing people from becoming authors. That would very easily create a
} culture where people are guarded and careful about where they help out,
} and may even foster an atmosphere of mistrust and feelings of being
} underappreciated. Personally I would much rather err on the side of
} being too generous, than risk leaving out people who in reality should
} have been included. If people generally feel secure in the knowledge
} that their contributions will be acknowledged properly, they will be
} more inclined to help out, and may in time also more easily feel that
} they can forgo co-authorships for what they consider "trivial" tasks.
} This makes for a more healthy scientific environment.
}
} Best regards
} Øystein
}
} -
} Øystein Prytz, PhD        |
} Professor of Physics      | Mobile: +47 93201512
} University of Oslo        | Office: +47 22840684
} oystein.prytz-at-fys.uio.no | http://folk.uio.no/oysteinp/group
}
}
} On 07.12.2020 21:35, xin-at-magnet.fsu.edu wrote:
} }
} }
} }
} } ----------------------------------------------------------------------------
} } The Microscopy ListServer -- CoSponsor:  The Microscopy Society of America
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} } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html
} } ----------------------------------------------------------------------------
} }
} } Hi, All,
} }
} } I would appreciate if you can give me some input on standard practice
} } concerning co-authorship on TEM data collection by the staff.
} } Is it appropriate for the staff to ask for co-authorship if the TEM staff
} } collects the TEM data and the TEM images are used in the publication? The
} } TEM user only pays the machine time, not the staff labor time.  There is
} } no data analysis involved.
} } Or in this case, the user should just acknowledge the staff with funding
} } information in the acknowledgement?
} }
} } Thanks very much
} } Yan Xin
} } FSU
} }
} } =====End of - Headers==============================


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From: microscopy.listserver-at-gmail.com
Date: Thu, 10 Dec 2020 06:38:44 -0600
Subject: [Microscopy] viaWWW:inquiry on standard practice on co-authorship

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: crya-at-loc.gov

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Email: crya-at-loc.gov Name: Cindy Connelly Ryan

Title-Subject: [Filtered] Fwd: Re: inquiry on standard practice on co-authorship

Message: I took a workshop earlier this year on exactly this topic - I suggest checking out the
flowcharts and discussion documents on the COPE website (Committee on Publication Ethics).
https://publicationethics.org/
Very helpful for working through who might only warrant an acknowledgement for a minor contribution
and who deserves co-authorship. Also rather harsh words about ghost, guest, and courtesy "authors".
So in the original poster's case, the question of whether that technician deserves a nod likely
centers on whether they did the bulk of your benchwork for you, or just a little bit of it.
(As a point of amusement - I am currently working on a review article summarizing how my lab's
approach to one particular topic has evolved since our founding in 1971, and I have 49 years worth
of possible people to credit! In many of those cases, posthumously. That will be a fun one to sort
out.)

best,

Cindy Connelly Ryan
Preservation Science Specialist
Library of Congress Preservation Research and Testing Division
Washington, D.C.


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From: microscopy.listserver-at-gmail.com
Date: Thu, 10 Dec 2020 17:28:33 -0600
Subject: [Microscopy] Fwd: viaWWW:inquiry on standard practice on

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Tobias Baskin {baskin-at-bio.umass.edu}


Greetings,
            When journal articles got started (I am thinking late 1600's) and for a while after,
authorship was easy. The author (usually just one) wrote the paper. Somewhere along the line the
concept morphed from the writer to the doer, usually more than one. Complexity ensued. The
guidelines that various societies have written up are helpful, if sometimes overly idealistic (for
example, I think it is unrealistic to demand that person in lab X be "responsible" for results from
lab Y in a paper that combines results from both). For me, I use the golden rule: If I had
contributed to the project in such and such a way, and I was *not* given authorship, would I feel
cheated? Admittedly subjective but works pretty well.
    Write on!
                        Tobias Baskin
Biology Department, UMass Amherst, USA

On 12/10/20 8:06 AM, microscopy.listserver-at-gmail.com wrote:
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} Email:crya-at-loc.gov Name: Cindy Connelly Ryan
}
} Title-Subject: [Filtered] Fwd: Re: inquiry on standard practice on co-authorship
}
} Message: I took a workshop earlier this year on exactly this topic - I suggest checking out the
} flowcharts and discussion documents on the COPE website (Committee on Publication Ethics).
} https://publicationethics.org/
} Very helpful for working through who might only warrant an acknowledgement for a minor contribution
} and who deserves co-authorship. Also rather harsh words about ghost, guest, and courtesy "authors".
} So in the original poster's case, the question of whether that technician deserves a nod likely
} centers on whether they did the bulk of your benchwork for you, or just a little bit of it.
} (As a point of amusement - I am currently working on a review article summarizing how my lab's
} approach to one particular topic has evolved since our founding in 1971, and I have 49 years worth
} of possible people to credit! In many of those cases, posthumously. That will be a fun one to sort
} out.)
}
} best,
}
} Cindy Connelly Ryan
} Preservation Science Specialist
} Library of Congress Preservation Research and Testing Division
} Washington, D.C.
}
}
} Login Host: 140.147.94.239
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From: microscopy.listserver-at-gmail.com
Date: Thu, 10 Dec 2020 17:29:01 -0600
Subject: [Microscopy] Fwd: viaWWW:inquiry on standard practice on

Contents Retrieved from Microscopy Listserver Archives
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X-from: Gregg Sobocinski {greggps-at-umich.edu}

Thank you, Cindy Connelly Ryan, for that reference.

It took me a bit to find the correct document, but it was
appropriately succinct, describing my situations as "ghost author" and
"gift author". Since department heads were specifically mentioned in
the article, I'm guessing that my gift author experience was not
unusual.
Here is the shortcut, for those who wish to go right to it:
https://publicationethics.org/files/2003pdf12_0.pdf

Regards,
~Gregg
Gregg Sobocinski
Microscope Imaging Suite, Managing Director
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA


On Thu, Dec 10, 2020 at 8:25 AM {microscopy.listserver-at-gmail.com} wrote:
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} Email: crya-at-loc.gov Name: Cindy Connelly Ryan
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} Title-Subject: [Filtered] Fwd: Re: inquiry on standard practice on co-authorship
}
} Message: I took a workshop earlier this year on exactly this topic - I suggest checking out the
} flowcharts and discussion documents on the COPE website (Committee on Publication Ethics).
} https://publicationethics.org/
} Very helpful for working through who might only warrant an acknowledgement for a minor contribution
} and who deserves co-authorship. Also rather harsh words about ghost, guest, and courtesy "authors".
} So in the original poster's case, the question of whether that technician deserves a nod likely
} centers on whether they did the bulk of your benchwork for you, or just a little bit of it.
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} of possible people to credit! In many of those cases, posthumously. That will be a fun one to sort
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}
} Cindy Connelly Ryan
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From: microscopy.listserver-at-gmail.com
Date: Thu, 10 Dec 2020 17:31:47 -0600
Subject: [Microscopy] viaWWW: Uranyl Acetate

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Email: andrew.sydor-at-sickkids.ca Name: Andrew Sydor

Title-Subject: [Filtered] Uranyl Acetate

Message: Hi everyone,

We are having a hard time finding uranyl acetate up here in Canada. Does anyone:
1) Know a Canadian supplier?
2) Know if this is a Canada-only problem or are other countries also experiencing this?
3) Know what the story is behind this issue (super curious)?

Thanks!
-Andrew

Andrew Sydor, PhD
Research Associate
Brumell Lab, Cell Biology Program
The Hospital for Sick Children
Peter Gilgan Centre for Research and Learning
686 Bay Street, Rm. 19.9400 Bench N-S East
Toronto ON M5G 0A4
P 416.813.6626

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From: matthew.weyland-at-monash.edu
Date: Thu, 10 Dec 2020 17:46:14 -0600
Subject: [Microscopy] Re: inquiry on standard practice on co-authorship

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Dear Yan,

Each lab and university obviously sets their own policy, but I do have
some recommendations.

All facilities should require acknowledgments for use of their
equipment, whether the user pays for just instrument time or a full
service. This is a really easy argument to make. If the users are making
use of your facility it means they need it, and will want it in the
future and will want it to be excellent and well equipped.
Acknowledgements make it easy to track the publication impact of usage
and in doing so this invests in the future of the facility. So the
argument you make to your users is "Do you want this facility to be here
next year?", " Do you want us to be able to invest in equipment and
staff that make your research easier?", "Do you want to avoid having to
outsource experiments, and great inconvenience and cost?". An
acknowledgement is not a large price to pay as a future investment!

The other argument you can make is that users do not pay the full cost
and that their work is subsidised by the institution, and as such they
need to acknowledge this. This is a negative argument and not as useful
as the above. I have long been a fan of billing users the FULL costs of
their microscope and staff time, and then adding a subsidy line to the
bill so it is explicit who is actually paying.

Regarding co-authorship that is a grey area. As an academic (and a
facility scientist) I am able to require co-authorship after a certain
amount of work, usually if I spend more than a day on a users research
(not training) this is, as far as I am concerned non-negotiable. But I
appreciate that most facility staff are not in my position. As soon as
staff have an intellectual investment in a project, beyond their day to
day duties I feel this should be a co-authorship. Doing something new,
development tasks anything that takes more time than the routine should
be acknowledged with co-authorship. For small extra work a personalised
mention in the acknowledgements is polite, but often this gets forgotten.

In your situation below I think the staff, if they are doing routine
microscopy and this is part of their job, and not any special analysis,
should not expect co-authorship. A personal acknowledgement would be
polite, but a facility acknowledgement should be expected. The time
factor is also an issue, if your staff are going above and beyond
regarding the amount of time on one project, and in doing so offering
intellectual input top a project this is a different matter.

Good luck,

Matthew

On 07.12.2020 21:35, xin-at-magnet.fsu.edu wrote:
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} Hi, All,
}
} I would appreciate if you can give me some input on standard practice
} concerning co-authorship on TEM data collection by the staff.
} Is it appropriate for the staff to ask for co-authorship if the TEM staff
} collects the TEM data and the TEM images are used in the publication? The
} TEM user only pays the machine time, not the staff labor time. There is
} no data analysis involved.
} Or in this case, the user should just acknowledge the staff with funding
} information in the acknowledgement?
}
} Thanks very much
} Yan Xin
} FSU

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From: microscopy.listserver-at-gmail.com
Date: Fri, 11 Dec 2020 16:51:55 -0600
Subject: [Microscopy] viaWWW: Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
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X-from: Sgkcck {sgkcck-at-aol.com}



Good morning Regulations on Uranyl Acetate have become very tough.  It is not that there is a
shortage of it we are making it and have it the question becomes does your facility allow for
Depleted Uranium on site.
We have ample supply to ship so please let us know how we can help to get you what you need.  As
well we offer and this may be of assistance to you and anyone else Uranyl replacements such as
Uranyless and UAR .
We are here to discuss the shipping of UA and any of the replacements s to you

Thank you and we look forward to hearing from you

Stacie Kirsch
Electron Microscopy Sciences
1560 Industry Road
Hatfield Pa 19440
E:Mail: stacie-at-ems-secure.com  or info-at-emsdiasum.com
Tel: 215-412-8400 Fax: 215-412-8450
www.emsdiasum.com


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Email: andrew.sydor-at-sickkids.ca {mailto:andrew.sydor-at-sickkids.ca} Name: Andrew Sydor

Title-Subject: [Filtered] Uranyl Acetate

Message: Hi everyone,

We are having a hard time finding uranyl acetate up here in Canada. Does anyone:
1) Know a Canadian supplier?
2) Know if this is a Canada-only problem or are other countries also experiencing this?
3) Know what the story is behind this issue (super curious)?

Thanks!
-Andrew

Andrew Sydor, PhD
Research Associate
Brumell Lab, Cell Biology Program
The Hospital for Sick Children
Peter Gilgan Centre for Research and Learning
686 Bay Street, Rm. 19.9400 Bench N-S East
Toronto ON M5G 0A4
P 416.813.6626

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From: microscopy.listserver-at-gmail.com
Date: Fri, 11 Dec 2020 16:52:18 -0600
Subject: [Microscopy] Fwd: viaWWW: Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
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X-from: Brandon Drescher {brandon.drescher-at-gmail.com}




Hi Andrew,

The lab I was in at Harvard had heard that it was becoming more difficult to get UA in Canada.  We
don't know of any supplier in Canada as we purchase nearly everything from Electron Microscopy
Sciences.  Canada is likely going the route that Japan took in severely limiting the use of UA. 
There is a group at the University of Toronto you might reach out to that uses UA, at least for
HPF-FS (ref - Mulcahy et al. Frontiers in Neural Circuits 2018).

You might want to consider alternatives such as gadolinium acetate and samarium acetate (refs:
Nakakoshi et al. JEM 2011; Odriozola et al. bioRxiv 2017) which have shown promise particularly as
/en bloc/ stains.  You could also try UranyLess (from EMS - not sure about a Canadian supplier for
this), but I have found it useful only for post-staining on grids, not for /en bloc/ work (if that's
a concern).

Best,

Brandon

On Thu, Dec 10, 2020 at 6:52 PM {microscopy.listserver-at-gmail.com
{mailto:microscopy.listserver-at-gmail.com} } wrote:




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Email: andrew.sydor-at-sickkids.ca {mailto:andrew.sydor-at-sickkids.ca} Name: Andrew Sydor

Title-Subject: [Filtered] Uranyl Acetate

Message: Hi everyone,

We are having a hard time finding uranyl acetate up here in Canada. Does anyone:
1) Know a Canadian supplier?
2) Know if this is a Canada-only problem or are other countries also experiencing this?
3) Know what the story is behind this issue (super curious)?

Thanks!
-Andrew

Andrew Sydor, PhD
Research Associate
Brumell Lab, Cell Biology Program
The Hospital for Sick Children
Peter Gilgan Centre for Research and Learning
686 Bay Street, Rm. 19.9400 Bench N-S East
Toronto ON M5G 0A4
P 416.813.6626

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--
Brandon Drescher
Postdoctoral Research Associate
Brown University
Department of Molecular Microbiology and Immunology
Box G-B5
Providence, RI 02912

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From: nizets2-at-yahoo.com
Date: Wed, 16 Dec 2020 09:22:25 -0600
Subject: [Microscopy] Advice on how to prepare viral particles bound to mineral particles

Contents Retrieved from Microscopy Listserver Archives
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Dear colleagues,


I want to image coronavirus particles sitting on dust particles by SEM.
I intend to capture the dust particles on a filter for easy washing, dehydrate and sputter coat the filter.

I will start with a virus suspension so at some point I need to wash and dehydrate.
I am not sure if virus particles withstand drying from water without fixation.
What would you recommend me? Fixation? Any of dehydration process?
Thank you in advance!


Stephane


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From: microscopy.listserver-at-gmail.com
Date: Wed, 16 Dec 2020 15:39:43 -0600
Subject: [Microscopy] viaWWW: TEM - Oil is leaking for ODP pump

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X-from: ravithakkar-at-ksu.edu

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Email: ravithakkar-at-ksu.edu Name: Ravindra Thakkar

Title-Subject: [Filtered] TEM - Oil is leaking for ODP pump

Message: Hi,
I am using Tecnai12 Biotwin 120 kV TEM. The machine is not getting a vacuum in the projection
chamber. I find out that oil is leaking from the ODP pump to the buffer tank.

Is anyone came across this issue? What makes an oil leak and how should I prevent it?

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From: microscopy.listserver-at-gmail.com
Date: Wed, 16 Dec 2020 15:50:04 -0600
Subject: [Microscopy] viaWWW: TEM - Oil is leaking for ODP pump

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X-from: Panagiotis Berillis {pveril-at-apae.uth.gr}

Ravindra have you checked the sealing O-rings from the ODP pump? There must be 2 O-rings. One to the
inlet point of the oil to the ODP and one to the draining point of the ODP. Sometimes, due to high
temperatures, these O-rings are damaged and need replacement. Damaged O-rings can cause oil leaking.
Panos

_________________________________________________________________
Dr. Panagiotis Berillis
Associate Professor Microscopy and Image Analysis in Histology and in Aquatic Organisms Department
of Ichthyology and Aquatic Environment
School of Agricultural Sciences University of Thessaly Hellas (Greece)
Tel: (+30)2421093248, (+30)6909711300

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Email: ravithakkar-at-ksu.edu Name: Ravindra Thakkar

Title-Subject: [Filtered] TEM - Oil is leaking for ODP pump

Message: Hi,
I am using Tecnai12 Biotwin 120 kV TEM. The machine is not getting a vacuum in the projection
chamber. I find out that oil is leaking from the ODP pump to the buffer tank.

Is anyone came across this issue? What makes an oil leak and how should I prevent it?

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From: microscopy.listserver-at-gmail.com
Date: Wed, 16 Dec 2020 15:56:51 -0600
Subject: [Microscopy] Fwd: Fwd: viaWWW: Uranyl Acetate

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-from: Jeffrey Streger {jeff-at-ravescientific.com}

Dear All,

You can get Uranyless from Edge Scientific in Canada.

See this link:

https://edgescientific.com/product/uranyless-uranium-free-aqueous-staining-solution-for-leica-em-stain-200ml-bottle/


In addition you can also get this from our web site in the United States.

https://ravescientific.com/rave-shop/category/uranyless-staining



With best regards,

Jeff Streger
Principal

8 Heller Park Lane Somerset, NJ 08873
Telephone: 732-672-4840
E-mail: Jeff-at-ravescientific.com Web site: www.ravescientific.com {http://www.ravescientific.com}




On 12/11/20, 6:14 PM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote:

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X-from: Brandon Drescher {brandon.drescher-at-gmail.com}
Hi Andrew,
The lab I was in at Harvard had heard that it was becoming more difficult to get UA in
Canada. We don't know of any supplier in Canada as we purchase nearly everything from Electron
Microscopy Sciences. Canada is likely going the route that Japan took in severely limiting the
use of UA. There is a group at the University of Toronto you might reach out to that uses UA,
at least for HPF-FS (ref - Mulcahy et al. Frontiers in Neural Circuits 2018).
You might want to consider alternatives such as gadolinium acetate and samarium acetate
(refs: Nakakoshi et al. JEM 2011; Odriozola et al. bioRxiv 2017) which have shown promise
particularly as /en bloc/ stains. You could also try UranyLess (from EMS - not sure about a
Canadian supplier for this), but I have found it useful only for post-staining on grids, not for
/en bloc/ work (if that's a concern).
Best,
Brandon
On Thu, Dec 10, 2020 at 6:52 PM {microscopy.listserver-at-gmail.com
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Email: andrew.sydor-at-sickkids.ca {mailto:andrew.sydor-at-sickkids.ca} Name: Andrew Sydor
Title-Subject: [Filtered] Uranyl Acetate
Message: Hi everyone,
We are having a hard time finding uranyl acetate up here in Canada. Does anyone:
1) Know a Canadian supplier?
2) Know if this is a Canada-only problem or are other countries also experiencing this?
3) Know what the story is behind this issue (super curious)?
Thanks!
-Andrew
Andrew Sydor, PhD
Research Associate
Brumell Lab, Cell Biology Program
The Hospital for Sick Children
Peter Gilgan Centre for Research and Learning
686 Bay Street, Rm. 19.9400 Bench N-S East
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From: diller-at-stefan-diller.com
Date: Wed, 16 Dec 2020 16:11:28 -0600
Subject: [Microscopy] Re: Fwd: viaWWW: TEM - Oil is leaking for ODP pump

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Hello Ravindra,

if diffusion pump oil is spread to the buffer tank that mostly means that there is a big vacuum leak somewhere in the pumped
volume of the TEM.

You have to search for leaks...

There should be a paragraph in the manual for this.


Best wishes,

Stefan

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Stefan Diller - Scientific Photography
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Am 16.12.20 um 22:56 schrieb microscopy.listserver-at-gmail.com:
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} X-from: Panagiotis Berillis {pveril-at-apae.uth.gr}
}
} Ravindra have you checked the sealing O-rings from the ODP pump? There must be 2 O-rings. One to the
} inlet point of the oil to the ODP and one to the draining point of the ODP. Sometimes, due to high
} temperatures, these O-rings are damaged and need replacement. Damaged O-rings can cause oil leaking.
} Panos
}
} _________________________________________________________________
} Dr. Panagiotis Berillis
} Associate Professor Microscopy and Image Analysis in Histology and in Aquatic Organisms Department
} of Ichthyology and Aquatic Environment
} School of Agricultural Sciences University of Thessaly Hellas (Greece)
} Tel: (+30)2421093248, (+30)6909711300
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} -----Original Message-----
} X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday,
} December 16, 2020 11:40 PM
} To: pveril-at-uth.gr
} Subject: [Microscopy] viaWWW: TEM - Oil is leaking for ODP pump
}
}
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} Email: ravithakkar-at-ksu.edu Name: Ravindra Thakkar
}
} Title-Subject: [Filtered] TEM - Oil is leaking for ODP pump
}
} Message: Hi,
} I am using Tecnai12 Biotwin 120 kV TEM. The machine is not getting a vacuum in the projection
} chamber. I find out that oil is leaking from the ODP pump to the buffer tank.
}
} Is anyone came across this issue? What makes an oil leak and how should I prevent it?
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From: microscopy.listserver-at-gmail.com
Date: Wed, 16 Dec 2020 16:15:38 -0600
Subject: [Microscopy] viaWWW: TEM - Oil is leaking for ODP pump

Contents Retrieved from Microscopy Listserver Archives
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X-from: Ravindra Thakkar {ravithakkar-at-vet.k-state.edu}


Yea, I changed the O-rings, but it didn't work.

With Thanks and Regards.

--------------------------------------

Ravindra Thakkar

Associate Scientist

Nanotechnology Innovation Center of Kansas State (NICKS),

Dept. of Anatomy & Physiology,

Kansas State University,

1800 Denison Avenue, Mosier Hall K206,

Manhattan, KS -66506

Tel. (785) 532 4742

Fax (785) 532 4953

Cell(785) 210 6108

E-mail: ravithakkar-at-vet.k-state.edu

http://nicks.ksu.edu/electron-microscopy/

------------------------------------------

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*To:* MicroscopyListServer-Forward {microscopy-at-microscopy.com} ; ravithakkar-at-ksu.edu
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*Subject:* Fwd: [Microscopy] viaWWW: TEM - Oil is leaking for ODP pump
X-from: Panagiotis Berillis {pveril-at-apae.uth.gr}

Ravindra have you checked the sealing O-rings from the ODP pump? There must be 2 O-rings. One to the
inlet point of the oil to the ODP and one to the draining point of the ODP. Sometimes, due to high
temperatures, these O-rings are damaged and need replacement. Damaged O-rings can cause oil leaking.
Panos

_________________________________________________________________
Dr. Panagiotis
Berillis
Associate Professor Microscopy and Image Analysis in Histology and in Aquatic Organisms Department
of Ichthyology and Aquatic Environment
School of Agricultural Sciences University of Thessaly Hellas (Greece)
Tel: (+30)2421093248, (+30)6909711300

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Email: ravithakkar-at-ksu.edu Name: Ravindra Thakkar

Title-Subject: [Filtered] TEM - Oil is leaking for ODP pump

Message: Hi,
I am using Tecnai12 Biotwin 120 kV TEM. The machine is not getting a vacuum in the projection
chamber. I find out that oil is leaking from the ODP pump to the buffer tank.

Is anyone came across this issue? What makes an oil leak and how should I prevent it?

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From: microscopy.listserver-at-gmail.com
Date: Thu, 17 Dec 2020 17:04:52 -0600
Subject: [External] [Microscopy] Advice on how to prepare viral particles bound to mineral particles

Contents Retrieved from Microscopy Listserver Archives
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}

Stephane,

I would suggest not dehydrating, etc. Freeze-fix - high-pressure freezing if you have access to that
- followed by cryocoating with Pt or Ir and examing with a cryostage.
If you can't cryocoat or don't have a cryostage, then freeze-dry (not in a lyophilizer) and coat
with Pt or Ir. Not a regular sputter coater.

Phil
-----------------------------------------
Philip Oshel
Imaging Center Director
Biology Department
1304 Biosciences
1455 Calumet Ct.
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576 office​
(989) 774-7567 lab



________________________________________
X-from: nizets2-at-yahoo.com {nizets2-at-yahoo.com}
Sent: Wednesday, December 16, 2020 10:27
To: Oshel, Philip Eugene

Dear colleagues,


I want to image coronavirus particles sitting on dust particles by SEM.
I intend to capture the dust particles on a filter for easy washing, dehydrate and sputter coat the
filter.

I will start with a virus suspension so at some point I need to wash and dehydrate.
I am not sure if virus particles withstand drying from water without fixation.
What would you recommend me? Fixation? Any of dehydration process?
Thank you in advance!


Stephane


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7, 52 -- Subject: Advice on how to prepare viral particles bound to mineral particles
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19, 54 -- Subject: Fwd: [External] [Microscopy] Advice on how to prepare viral particles
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