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Email: mlynn-at-ameslab.gov Name: Matthew Lynn
Organization: Ames Laboratory, Dept. of Energy
Title-Subject: [Filtered] TEM: Postdoctoral position at the Ames Laboratory
Message: The Division of Materials Science and Engineering (DMSE) at the Ames Laboratory, a Department of Energy National Laboratory affiliated with Iowa State University, is searching for a qualified Postdoctoral Research Associate.
This is an opportunity to support a diverse range of research programs using advanced aberration-corrected STEM and associated techniques. More details and application instructions can be found here:
From fadecice330ojoi-at-gmail.com Mon Jan 6 07:32:29 2020 Return-Path: {fadecice330ojoi-at-gmail.com} Received: from gmail.com (a3.68474.cn [23.228.73.183] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 006DWSaq013173 for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 6 Jan 2020 07:32:28 -0600 Message-ID: {029F40E5.7510914E-at-gmail.com}
Dear Colleagues,
It's the beginning of the new year and it's time to plane your future training opportunities!
We are excited to be offering the first, of what promises to be annual, Big Data, Big Problems light-sheet workshop, held at Princeton University, Princeton, NJ, USA.  The course will run July 27-July 31, 2020.
Applications are due April 15, 2020.  Apply through the course web site at BigDataBigProblems.com.
Multi-dimensional live and fixed microscope data can be collected in so many ways, and the various solutions seem to grow almost daily.  There are many courses that focus on general optical principals and the use of conventional microscope platforms such as widefield fluorescence, confocal and multiphoton imaging, but none that are specifically designed to demystify rapidly evolving and increasingly prescient methods such as light-sheet imaging.  Ultimately this is the goal of this course, what is light-sheet microscopy in all its flavors, what can you do with it, how do you choose between platforms and once you have a system or are proficient with a device what do you do with the data?  This week long course brings together experts in conventional optics, all aspects of light sheet microscopy and image analysis to help you bring your light-sheet based cellular, animal and large sample/cleared sample imaging to the next level.  From the syllabus you will see that we start out with principals and choices and then move into specific systems instructed by both academic and industry faculty.  In fact, we have will have almost all the available commercial solutions on site for you to use.  We will provide samples, but equally you are welcome to use your own.   Our goal is that you will return to your home institution fully capable to implement and use these truly exciting new tools.
Course Speakers;
Amy Elliot National Institutes of Health
Holly Gibbs Texas A&M
Elizabeth Hillman Columbia University
Jan Huisken Morgridge Institute for Research
Gary Laevsky Princeton University
Talley Lambert Harvard University
Wesley Legant University of North Carolina
Paul Maddox University of North Carolina
Alison North The Rockefeller University
Eszter Posfai Princeton University
Doug Richardson Harvard University
Kelly Seagraves Princeton University
Hari Shroff National Institutes of Health
Claudette St. Croix University of Pittsburgh
Sebastian Streichan University of California Santa Barbara
Jared Toettcher Princeton University
Simon Watkins University of Pittsburgh
  Applications are due April 15, 2020.  Apply through the course website, BigDataBigProblems.com
  Course Directors
Gary Laevsky, Princeton University
Simon Watkins, University of Pittsburgh
--
Best,
Gary Laevsky, Ph.D. Director, Confocal Imaging Facility Nikon Center of Excellence Co-Founder, North Atlantic Microscopy Society (NAMS)  https://namsmicroscopy.com/ Dept. of Molecular Biology Washington Rd. Princeton University  Princeton, New Jersey, 08544-1014 (O) 609 258 5432 (C) 508 507 1310
North Atlantic Microscopy Society Spring Meeting at UPENN, April 23, 2020.
==============================Original Headers============================== 41, 37 -- From gary_laevsky-at-yahoo.com Mon Jan 6 07:51:12 2020 41, 37 -- Received: from sonic315-13.consmr.mail.bf2.yahoo.com (sonic315-13.consmr.mail.bf2.yahoo.com [74.6.134.123]) 41, 37 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 006DpBht013837 41, 37 -- for {microscopy-at-microscopy.com} ; Mon, 6 Jan 2020 07:51:12 -0600 41, 37 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1578314901; bh=rX7HfAq0Y2/NoJUTDdnvnFAviZgA0eej4+RFezJCCeM=; h=Date:From:To:Subject:References:From:Subject; b=OXcV0x0ZCFfA6d317PBpVbvWlhbevchds3nTIafEqV6Y9f6mpKf/Iz9s7GmpDaU3OKzd78jAeiFGdxWI60CTML4aKcLsI7TPVHwVrtRBD8cVKkgu5c2woinJ0pec6JZptOz0NHxzLpE8J5ZtpkcWgyOViUuOu5zbTYj0uXymAOg/YJLUqO+fPKg9aKFegW9LSuNHCgOisO7jGtpJMxwJ1w66X+AlO0V3cPWf8GqX+oTRXsOwxajyJZOqHgl2VJRvwCmF9Iia5nraq+S5I6A+nsKvR+zEZ3yWub+zcPq8M+1RQpyHvtZf7lgb1mRPYCbDqDEHyrqSUaPGDBEQpLaaKg== 41, 37 -- X-YMail-OSG: mdZJxeAVM1nFjQCN23ybY5ckxENFiF1gEzEVsHJ2ea2bEkxL874ESTqP9oKpld2 41, 37 -- w9VpANKCGFxTiS2ZQOlrG8ETv3qE_LCSFxjbk1.Ig_crPYbLYkQ83MocVZx198O7KK6XELM4nao0 41, 37 -- 5kAeFRJrImnq9jkEoeLNtEcffzXRywTAbXJASoMqXvET8mEySlzO9rXoo5eAhmXQc3x7ptCrIN0c 41, 37 -- 4EwMVdVtVquE8lAxuefUeUafQatqiAUYl10XabTWP3vH3xebv.uVaa6CsXQyAtwi.9UQyfgV6QHZ 41, 37 -- m959uGbqGvuIs2b9IgAgEYslI2zCR2QGxvkwrqvxj.ygkJ4o0a9vD.Bk07VL20hzPAEYyYywHJY. 41, 37 -- HLInwIrC8BT8jyksQLaGu7LNmgpAuANHEh1.se0tFVgxNCSS3uJpKwco9b3LgIFHkH5MiDMLJ_rs 41, 37 -- .sDUcyQPi7yIghNsXpdtO0IFgcaF78SgjnMFD5GCrjsqSlk7cedqLz_GxDRI1ooWPq2_0ejMODOT 41, 37 -- KUxSIf90r9fcy57iFhHOog.u.jdCh_gUgoTQEXzre.KC0lhrwnJfKjci0Lw3TzkD3uHBrRdl6YaI 41, 37 -- o_umD6xk9kz6WicbUY83kazuuzJq_i5IyvgLoM319E3DJPNwAR8YmDxSP.N96YWH76XmP7U5QsuP 41, 37 -- VrA9KEGH24cpNqjvj4xmYTjWSYpPFGAhz13cnQ8UcCm4It9_qbvbfa9pVn76_XvUMieCYyUalPbx 41, 37 -- jExCWiDMsyvEVYN0ZGXhVMWmoKUsqcok6IARB_bHtIsTHPQV85ImfqGUZqO8USy.v2.w2FIGxxwC 41, 37 -- X8R8YAtkGjv5H4la6ZGxxxgpfyV90qyeX65wiRLq6z3wKmFhMzM2rPeE87JLlmXL_jNH459RQx1u 41, 37 -- 7x.8tR7O6nJI6L8aEdmrCziP32im5FjfEYKWZvBcsYnFO7eMlWAKk.jypbH6r4RoFfI928JiX7E8 41, 37 -- FVOSbKhXymt5Wk0W9KHX5Fy26TjnAALu36y_G4VhZprobUC7vXuBxoD_i19TbOqHuZRdMfCAVOVu 41, 37 -- l7XH4ni_Vm7BDXIso81d6aDb15HVQIkrS6lHhvnNWH7eExpednpUd2.QrlW1Pn5pSqKqw8OcT_eo 41, 37 -- IKRkHieQKoMltE2JI6tRJI3xvD8VoqmHUJdDUTIz3GFOrJoc69_p5kKnNmW1AHcCiUnuYUKKqtAx 41, 37 -- 2ZOntyh9rOxfPNzi0hjGWHgm1Q.3aOKif1Bg2VITpdMFHTlGWN8rtvHfIDp1g_sgEfvu93LuUjvC 41, 37 -- ZV8FO38msGslorEusPO0tOt02.d3D6lPttfAeg3.hQZpfnzRvSsWrDxqp9sXLZ.QqcDw2NYts0n7 41, 37 -- xmJ4llhZpeOhApgAdNXUG 41, 37 -- Received: from sonic.gate.mail.ne1.yahoo.com by sonic315.consmr.mail.bf2.yahoo.com with HTTP; Mon, 6 Jan 2020 12:48:21 +0000 41, 37 -- Date: Mon, 6 Jan 2020 12:48:19 +0000 (UTC) 41, 37 -- From: Gary Laevsky {gary_laevsky-at-yahoo.com} 41, 37 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 41, 37 -- Message-ID: {1153505689.4734631.1578314899793-at-mail.yahoo.com} 41, 37 -- Subject: Big Data, Big Problems; Light-sheet workshop Princeton University, 41, 37 -- July 27-31, 2020 41, 37 -- MIME-Version: 1.0 41, 37 -- Content-Type: text/plain; charset=UTF-8 41, 37 -- References: {1153505689.4734631.1578314899793.ref-at-mail.yahoo.com} 41, 37 -- X-Mailer: WebService/1.1.14873 YMailNorrin Mozilla/5.0 (Macintosh; Intel Mac OS X 10.13; rv:71.0) Gecko/20100101 Firefox/71.0 41, 37 -- Content-Transfer-Encoding: 8bit 41, 37 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 006DpBht013837 ==============================End of - Headers==============================
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Title-Subject: [Filtered] question about fib milling parameters on polymer membrane
Message: Dear Colleagues,
First at all, Happy New Year !
then, I have a question for you : i started my phd in last october and i work on polymer membrane. My goal is to image, in a fist time, the 3D structure of my membrane. For this, i want to use the FIB/SEM available in my lab. I know this is fragile sample and i need to adapt my parameters for milling my surface without damage them.
This is my question : Someone alrady use this technique on polymer membrane ? And have an idea of optimal parameters (current, energy, deep, dual time..) for use the ion beam ? I have PES and PAN membranes. I need reference parameters for this type of sample.
Thanks a lot and have a nice day
Best regards
HÈlËne Roberge Phd student IMN - Institut des MatÈriaux Jean Rouxel 2, rue de la HoussiniËre - BP 32229 44 322 NANTES CEDEX 3 https://www.cnrs-imn.fr/ 0240376316
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I’m excited to announce that I’ll be hosting the spring 2020 meeting of the North Atlantic Microscopy Society (NAMS) here at the Perelman School of Medicine at the University of Pennsylvania on April 23rd.
NAMS was founded by Gary Laevsky and Paul Shao at Princeton University. Its mission is to promote microscopy education and to provide researchers and vendors in the region with opportunities to connect and hear about the latest applications of microscopy techniques. 
The focus of this meeting will be Correlative Light-Electron Microscopy (CLEM). Light and electron microscopy (EM) speakers will highlight their respective techniques, while CLEM speakers will demonstrate the benefits of bringing the two modalities together.
You can register for the meeting here: https://namsmicroscopy.com/meeting-registration
I hope to see some of you here in April!
Andrea
Andrea L. Stout, Ph. D. Director,  CDB Microscopy Core Facility Perelman School of Medicine at the University of Pennsylvania 1107 BRB 2/3 421 Curie Boulevard Philadelphia, PA 19104
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Title-Subject: [Filtered] Reminder EGU 2020: Advances in microanalysis: Insights into nanoscale trace element heterogeneities
Message: Dear colleagues,
We would like to invite submissions of abstracts to our EGU 2020 session applying advanced microanalysis techniques to investigate chemical heterogeneities.
GMPV1.3 "Advances in microanalysis: Insights into nanoscale trace element heterogeneities" Convener: Renelle DubosqECS | Co-conveners: Tyler BlumECS, Sandra Piazolo
Invited speakers: Dr. Desmond Moser, Western University, will present on trace elements and microstructures from Early Mars and the geochronology of habitability
Dr. Ana Cernok, Royal Ontario Museum, will present on shock‐induced microtextures in lunar apatite and merrillite
Please follow this link: https://meetingorganizer.copernicus.org/EGU2020/session/35196 for a full description of the session.
Abstract submission (deadline: 15 January 2020, 13:00 CET)
Looking forward to seeing you in Vienna, Best regards,
Renelle, Tyler and Sandra
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Email: johnshields59-at-gmail.com Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Biological TEM Workshop, March 11
Message: *Biological TEM Workshop* This intensive, three-day workshop provides a practical and basic theoretical introduction to the Transmission Electron Microscope and biological sample preparation techniques. Each day will consist of lecture, discussion and *hands-on* training led by GEM staff. Who: Anyone requiring training on TEM and biological sample preparation. The workshop will be limited to 6 participants based on the availability of equipment. When: Wednesday through Friday, March 11-13 2020, 8am-5pm each day (lunch is provided) Where: 154 Barrow Hall, University of Georgia, Athens, GA 30602
Registration: Contact John Shields (jpshield-at-uga.edu) for more information and to sign up. Registration requires iLab account through the GEM website. https://uga.ilabsolutions.com/account/login Deadline: March 4, 2020
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Email: john.kron-at-atlanticexperts.com Name: John Kron
Organization: Atlantic Professional Services
Title-Subject: [Filtered] jeol JSM 6360 SEM
Message: Does anyone have any information on error codes, specifically error code 147 Vacuum system failure
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one of my customers is producing active carbon, I suppose, from different kind of wood.
Is there any (SEM microscopic) way to find out the active surface value in square meters per gram of carbon? In a more precise way than to estimate it?
Thanks, and happy new year
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
My understanding is that the pore structure would push the limit of SEM to characterize it. I don't think there would be any reliable way to correlate the microstructure with an estimate of specific surface area. And if the operator or SEM was having an off day, they would fail to resolve the porosity and thus fail to properly compare samples.
I recall that BET is the norm for measuring specific surface. I presume it works for activated carbon. I think it would give much more reliable number than anything that could be done microscopically.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: diller-at-stefan-diller.com {diller-at-stefan-diller.com} Sent: Wednesday, January 08, 2020 5:18 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
Dear All,
one of my customers is producing active carbon, I suppose, from different kind of wood.
Is there any (SEM microscopic) way to find out the active surface value in square meters per gram of carbon? In a more precise way than to estimate it?
Thanks, and happy new year
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Apologies for the double post, but the deadline for our advertised 3-year postdoc position in STEM imaging at Monash University has been extended to Friday 24th January. If you know anyone interested who may not have been able to apply over the holiday period, there's still time.
Full details at: http://careers.pageuppeople.com/513/cw/en/job/600527/research-fellow-in-atomicscale-structure-determination-in-thick-nanostructures
Kind regards, Scott
On 22/11/2019 8:08 am, Scott Findlay wrote: } Dear colleagues, } } I'm advertising a 3-year postdoc position in atomic-scale structure } determination in thick nanostructures at the School of Physics and } Astronomy, Monash University, Australia.  Further details below. } } Please bring this to the attention of anyone who may be interested. } } Many thanks, } Scott Findlay } } ___________________ } } Position Descriptions and application details at: } http://careers.pageuppeople.com/513/cw/en/job/600527/research-fellow-in-atomicscale-structure-determination-in-thick-nanostructures } } } Position overview: The Research Fellow will work on developing methods } for atomic-scale structure determination via scanning transmission } electron microscopy. This project aims to develop a theoretical and } computational toolkit for structure retrieval at atomic resolution that } is robust in the presence of multiple scattering (“dynamical } diffraction”) of the electron probe, and to apply it to large } experimental data sets obtained from the new generation of fast-readout } electron detectors. The project may draw on methods from inverse } scattering theory, phase retrieval, iterative algorithms, machine } learning, and high-performance computing. } } Duration: 3-year fixed-term appointment } Remuneration package: $68,040 - $92,343 pa Level A (plus 17% employer } superannuation) } Closing date: Friday 10th January, 2020
-- *SCOTT FINDLAY* Senior Lecturer
*Monash University* School of Physics and Astronomy G08, New Horizons Centre, 20 Research Way, Clayton Campus Clayton, VIC 3800 Australia
From normanatlas54ykur-at-gmail.com Wed Jan 8 16:53:37 2020 Return-Path: {normanatlas54ykur-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [42.231.162.215] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 008Mrajs019679 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 8 Jan 2020 16:53:36 -0600 Received: from webmail.halftomorrow.com ([Thu, 09 Jan 2020 03:37:34 +0600]) by snmp.otwaloow.com with QMQP; Thu, 09 Jan 2020 03:37:34 +0600 Received: from unknown (HELO mxs.perenter.com) (Thu, 09 Jan 2020 03:26:46 +0600) by mail.webhostings4u.com with ASMTP; Thu, 09 Jan 2020 03:26:46 +0600 Received: from [162.44.252.169] by smtp18.yenddx.com with SMTP; Thu, 09 Jan 2020 03:24:53 +0600 Received: from unknown (38.247.92.211) by smtp4.cyberemailings.com with SMTP; Thu, 09 Jan 2020 03:11:34 +0600 Received: from rsmail.alkoholic.net ([126.90.161.200]) by nntp.pinxodet.net with QMQP; Thu, 09 Jan 2020 02:57:57 +0600 Message-ID: {64EC1C84.C2559439-at-gmail.com}
Hi Everyone,
We are in the midst of refurbishing an RMC MT-7000 ultramicrotome to replace our MT-7 teaching ultramicrotome that has developed a problem we haven't been able to fix. Unfortunately, the instrument I acquired is missing the horizontal overhead light (but luckily, not the mounting bar). RMC has been fantastic in support for this project, but doesn't have a light available that I can purchase. We've thought about modifying the light from the MT-7, but I still have hopes for getting it going again.
If anyone should happen to have an out of service MT-7000 and would be willing to part with the light fixture, curly cord and/or the plug for the light, please let me know. I have images of the overhead light available that I can send by regular email. The lamp from our other MT-7000 works perfectly with the unit under construction, so no worries about the rest of the circuit.
Thanks for any help, Heather
Heather A. Owen, Ph.D. Director, Electron Microscope Laboratory Department of Biological Sciences University of Wisconsin - Milwaukee 3209 N. Maryland Ave. Milwaukee, WI† 53211
Job Location: Johnson Matthey, Clean Air, Wayne, PA, USA
Job Description: The Development Analytical Services (DAS) Scientist II takes day-to-day responsibility for OM and SEM instrumentation and provides support to R&D projects that generate new products, processes and understanding of commercial value to JM while building an understanding of the science involved. The incumbent will perform routine analytical analyses using the scanning electron microscopy and a significant amount of non-routine work using existing analytical procedures that serve to characterize the properties, function, or composition of catalytic materials or materials of catalytic interest. The incumbent supports JM through all aspects of catalytical material discovery and delivery including analytical analysis, liaise with internal customers, and support of JM processes and practices. Individual will have responsibility for managing projects from inception to close.
For more details and how to apply: {https://chu.tbe.taleo.net/chu01/ats/careers/requisition.jsp?org=JOHNSONMATTHEY&cws=1&rid=9712} .
Louis Gambino, PhD Associate Scientist Johnson Matthey 436 Devon Park Drive Wayne, PA 19087-1816 If the reader of this email is not the intended recipient(s), please be advised that any dissemination, distribution or copying of this information is strictly prohibited. Johnson Matthey Inc. has its main place of business at 435 Devon Park Drive, Wayne, PA, 19087 USA. While Johnson Matthey aims to keep its network free from viruses you should note that we are unable to scan certain emails, particularly if any part is encrypted or password-protected, and accordingly you are strongly advised to check this email and any attachments for viruses. The company shall NOT ACCEPT any liability with regard to computer viruses transferred by way of email. Please note that your communication may be monitored in accordance with Johnson Matthey internal policy documentation.
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Email: chrisbrantner-at-gwu.edu Name: Chris Brantner
The Nanofabrication and Imaging Center at George Washington University will be holding its 4th annual CLEM workshop June 8-12, 2020 in Washington DC. If you are interested in applications and workflow for CLEM projects, then this workshop is for you.
Please visit the website for further details.
https://nic.gwu.edu/clem-workshop
Happy New Year Chris Brantner
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Title-Subject: [Filtered] Upcoming Microscopy Workshops at EMS Microscopy Academy
Message: Materials Ultramicrotomy February 24, 2020 For those unfamiliar with microtomy to prepare for the workshop. February 25 - 27, 2020 This workshop will introduce individuals to the unique application of ultramicrotomy to materials. Learn more at https://www.emsmicroscopyacademy.com/product-page/materials-feb20 Aurion Immunogold Silver Staining April 15 - 17, 2020 Three days of hands-on training for students, researchers, and microscopists who want to learn the most up to date theory and practice in immunogold labeling. Learn more at https://www.emsmicroscopyacademy.com/product-page/immunogold-apr20 Cryosectioning/Immunogold April 20 - 24, 2020 Hands-on training for students, researchers, and microscopists who want to learn the most up to date theory and practice in cryosectioning and immunogold labeling. Learn more at https://www.emsmicroscopyacademy.com/product-page/cryoimmuno-apr20
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The Analytical Instrumentation Facility (AIF) and Research Triangle Nanotechnology Network (RTNN) at North Carolina State University, in partnership with Protochips and ThermoFisher Scientific, are pleased to announce the first annual In Situ Microscopy Congress (ISMC). The meeting will be held at North Carolina State University in Raleigh, NC on March 2nd and 3rd, 2020.
This is a two day workshop which provides one the opportunity to learn about liquid phase and gaseous phase in situ capabilities, and includes two plenary talks from leaders in the respective fields along with hands-on demonstrations of liquid and gas cell holders and our Talos F200X, probe-corrected Titan, and Quanta 3D FIB/SEM platforms.
This workshop is open to all. Attendees are encouraged to submit an abstract to be considered for an oral or poster presentation. To register, or for more information, please visit the following registration link:
We look forward to seeing you, Chris -- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
==============================Original Headers============================== 6, 49 -- From crwinkler-at-ncsu.edu Mon Jan 13 18:14:24 2020 6, 49 -- Received: from mail-io1-f44.google.com (mail-io1-f44.google.com [209.85.166.44]) 6, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 00E0EOJ0001110 6, 49 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jan 2020 18:14:24 -0600 6, 49 -- Received: by mail-io1-f44.google.com with SMTP id t26so11721675ioi.13 6, 49 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jan 2020 15:11:59 -0800 (PST) 6, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 49 -- d=ncsu.edu; s=google; 6, 49 -- h=mime-version:from:date:message-id:subject:to; 6, 49 -- bh=jy/FXDxX1SDmv+M4BrhZrv6qZ1a/6WcuPc/gN88MZLA=; 6, 49 -- b=ZPgouZqxT/+9XFsRXj7H/A8k4ay8hTGSoIzfFnebjd7ZrcAIy163cU7wxl7NSw7WIl 6, 49 -- +LU7nk/pnnUWob+DUiGZYfnhceE9uiaABanwHMeNVJLuxKbX+atpooFiWjkIqQo20mRY 6, 49 -- N/39g5jv5qaJ3UBaLTH0DJzRXgacWYzGB+YikkEfkFnUPR5R1ZqTNktXbhJ8SbKzgxne 6, 49 -- SvtwRIE73/4RPbbpQxX8OQ7Ztex/dNq/XQWf1+PKozZY/rHrMq9swf8lyOmP2s4QQnRb 6, 49 -- UBWDvC5YAM8WqPEmTsZwOT2x/VQa8xjfftSpUhllquEja5NSQv4FANypZzkuPFQC0rEA 6, 49 -- xoig== 6, 49 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 6, 49 -- d=1e100.net; s=20161025; 6, 49 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 6, 49 -- bh=jy/FXDxX1SDmv+M4BrhZrv6qZ1a/6WcuPc/gN88MZLA=; 6, 49 -- b=UJ+dTbjUKyk0DfLdawyri+9VtbSJ8QuZx9bw1nyUIJWzvzFFxanwaSh+ifue8weLxu 6, 49 -- hr2Ruz16gtPhDaIv/pH7/9Z8jYcBLyWGMH1dmwqPJXe5bnXMqVTTKcoRvFPk6884kA/9 6, 49 -- HSJYxei27cPCVbpfwFxpsAQ4ISZv/MmsVCWchxNhKgKOIRQN4YInNn0SIBjbEQ7BZXkm 6, 49 -- DbPIW4+Glneq7ZMeQLzIdcZSFzFlub0sfzl/tRznPuKDhEmIepXCa1KwvfNpfzdz6N/z 6, 49 -- Svc4KNFJ47euTBWxt/NpDlX8rOV7ihpmU0j8dsH571zkm866aOsLVwycSgXbZVL2cvQU 6, 49 -- RAew== 6, 49 -- X-Gm-Message-State: APjAAAX+XwLPPXkhCrmAs0p/CO940k/usRGyiGU4qlKBz+BZi3B0myZ+ 6, 49 -- kDtj4ob7Eyll0G2hNamE6FLrmcl0wS01uDhxkuzHtvO0JAw3i7YOtxMBxYB64U9FdgpuYZXBd/r 6, 49 -- BAWHTnthUPLXm9Mla66Sw1wp5Aj6kDwSU/tsp53LK6hOAhohX1VCit8sJzqt7iR1ny/me 6, 49 -- X-Google-Smtp-Source: APXvYqy1fW+j+9EYk0Hh3NwBpJoNwUw7zxiLU3a4dAHmGVaFgsZDY/KVHo8YZ1/aSxjQeWPBZXz26w== 6, 49 -- X-Received: by 2002:a02:742:: with SMTP id f63mr16442709jaf.138.1578957118637; 6, 49 -- Mon, 13 Jan 2020 15:11:58 -0800 (PST) 6, 49 -- Received: from mail-io1-f49.google.com (mail-io1-f49.google.com. [209.85.166.49]) 6, 49 -- by smtp.gmail.com with ESMTPSA id x77sm4290851ilk.34.2020.01.13.15.11.58 6, 49 -- for {Microscopy-at-microscopy.com} 6, 49 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 6, 49 -- Mon, 13 Jan 2020 15:11:58 -0800 (PST) 6, 49 -- Received: by mail-io1-f49.google.com with SMTP id n21so11708956ioo.10 6, 49 -- for {Microscopy-at-microscopy.com} ; Mon, 13 Jan 2020 15:11:58 -0800 (PST) 6, 49 -- X-Received: by 2002:a05:6638:24f:: with SMTP id w15mr16827781jaq.130.1578957117827; 6, 49 -- Mon, 13 Jan 2020 15:11:57 -0800 (PST) 6, 49 -- MIME-Version: 1.0 6, 49 -- From: Christopher Winkler {crwinkler-at-ncsu.edu} 6, 49 -- Date: Mon, 13 Jan 2020 18:11:46 -0500 6, 49 -- X-Gmail-Original-Message-ID: {CAA8T2PP83eLbLQ3kuosXxrNeFSymOCtd2-9u3HVUCXEKKKoSUQ-at-mail.gmail.com} 6, 49 -- Message-ID: {CAA8T2PP83eLbLQ3kuosXxrNeFSymOCtd2-9u3HVUCXEKKKoSUQ-at-mail.gmail.com} 6, 49 -- Subject: In Situ Microscopy Congress - NCSU AIF - March 2nd and 3rd 6, 49 -- To: Microscopy-at-microscopy.com 6, 49 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
From NegativeSEOyrfxu-at-mailinator.com Mon Jan 13 20:51:42 2020 Return-Path: {NegativeSEOyrfxu-at-mailinator.com} Received: from mailinator.com (hn.kd.ny.adsl [42.231.162.213] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 00E2pfJH027377 for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 13 Jan 2020 20:51:42 -0600 Message-ID: {151756C7.AAB54645-at-mailinator.com}
X-from: tescanmicroscopy-at-gmail.com
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Email: tescanmicroscopy-at-gmail.com Name: dj miller
Organization: Tescan USA
Title-Subject: [Filtered] position available - Applications Specialist in FIB-SEM Tescan USA
Message: TESCAN USA, a fast-growing solution provider in electron, ion and x-ray CT in the microscope business, is looking for an Applications Specialist in FIB-SEM. This position is responsible for supporting the sales process of Tescan FIB-SEMs through tool demonstrations, sample runs, development of new techniques, contributions to technical papers and other applications activities. Qualified candidates will have extensive experience in Scanning Electron and Ion Microscopy and intimate familiarity with FIB-SEM tools and techniques such as TEM sample Preparation, 3D cross-sectioning and reconstruction (image, EDS & EBSD), delayering, nano-probing, circuit edit, lithography and other. Experience in other techniques such as TOF-SIMS, cryo sample preparation, APT, TEM would be a plus. Excellent communication in English is required. This position located in the Pleasanton, CA, but will require travel up to 50% of the time.
TESCAN USA will only employ individuals who are legally authorized to work in the United States for this opening (i.e. ñ no sponsorship is available). Any offer of employment may be conditional upon the successful completion of a background investigation and drug screening. TESCAN USA offers competitive salaries and benefits. Qualified candidates should send their resume, cover letter and salary requirements to: Gary.Hawkinson-at-tescan.com.
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Email: georg.ramm-at-monash.edu Name: Georg Ramm
Organization: Monash Ramaciotti Centre Title-Subject: [Filtered] Staff position CLEM and Bio EM at Monash University, Melbourne, Australia
Message: We are looking for a staff member with expertise in CLEM to join our team at the Monash Ramaciotti Centre at Monash University (https://www.monash.edu/researchinfrastructure/cryo-em) Senior Research Officer - Correlative Light and Electron Microscopy Location: Clayton campus, Melbourne, Australia
Employment Type: Full-time, 3 year Fixed-term appointment
Contact: Georg Ramm, Georg.ramm-at-monash.edu, +61-3 - 9905 1280
Closing date: 31 January 2020 Link to job advertisement: https://careers.pageuppeople.com/513/cw/en/job/599948/senior-research-officer-correlative-light-and-electron-microscopy
As the successful candidate you will:
Manage, plan, coordinate and oversee EM projects in collaboration with users Develop new protocols for correlative light and electron microscopy and life sciences EM techniques and apply them to research projects Teach specialised EM techniques such as CLEM and immuno EM to Monash researchers and to the wider Australian and international EM community Perform microscopy and related EM techniques including immuno EM, (cryo-) ultramicrotomy, high pressure freezing, and cryo-preparation
The Monash Ramaciotti Centre is a leading facility for life sciences electron microscopy. It houses Australiaís first Titan Krios microscope, a cryo-FIBSEM Helios G4 with Leica VCT500 cryo-stage, a Talos Arctica, as well as two 120keV TEMs and a FESEM. A suite of advanced sample preparation and other equipment is available, including a Zeiss LSM900 Airyscan with Linkam cryo-stage, a Wohlwend high pressure freezer, Leica AFS2 and FC7 cryo-ultramicroscopes. The facilityís expert team supports and collaborates on a large number of bio EM techniques ranging from standard SEM and TEM to immuno EM, correlative light and electron microscopy, cryotomography and single particle analysis.
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==============================Original Headers============================== 14, 54 -- From microscopy.listserver-at-gmail.com Mon Jan 13 21:37:40 2020 14, 54 -- Received: from mail-il1-f182.google.com (mail-il1-f182.google.com [209.85.166.182]) 14, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 00E3bege012610 14, 54 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2020 21:37:40 -0600 14, 54 -- Received: by mail-il1-f182.google.com with SMTP id s15so10132080iln.1 14, 54 -- for {microscopy-at-microscopy.com} ; Mon, 13 Jan 2020 18:35:15 -0800 (PST) 14, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 54 -- d=gmail.com; s=20161025; 14, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 14, 54 -- :in-reply-to:content-language:content-transfer-encoding; 14, 54 -- bh=Sn2y0f89Mh4r8oYttlALvoBIzs0SKEeNaQvfe58RP5s=; 14, 54 -- b=sxGh3u07OFI3bgsaUEu8jwzkQYmZq0BFhDcm3hGgNRrdfaBXJw6QNgm5dcitDJlZU0 14, 54 -- WqBy1rEBsYYmwuhrZBNmTWLYIBss5GlbwMq1+/WbNvlIAiNsnOjAkwyMqU+gbQqPk99D 14, 54 -- pUtJulK3Eg+I9Bq6JLbRBlOhnUptKCB/xjnEGLbzwfV1Ml1yAkIA1cWcQa5BPy3CgvI2 14, 54 -- aK+Ff8osk/KES6o+MS9NgAuC5D1WQEQ38cP5d7g0Oov017TfsntCtAQKdST3PLQHNBSY 14, 54 -- BAa4XIMxjzEe30DWLR144C4aTdovW+2MVE41wrnVXT5K5OV8lihenYc9cYBuN7F7HFkt 14, 54 -- 1v+g== 14, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 14, 54 -- d=1e100.net; s=20161025; 14, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date 14, 54 -- :user-agent:mime-version:in-reply-to:content-language 14, 54 -- :content-transfer-encoding; 14, 54 -- bh=Sn2y0f89Mh4r8oYttlALvoBIzs0SKEeNaQvfe58RP5s=; 14, 54 -- b=gdhM5F7B3vaEextjM8o9G5t4w9TCzoc7jDFFKBCTiItXavneUrKnKyWX3xB/rh7AvB 14, 54 -- 71umfj7Ffihi9fEVILpnPoTS3bkDBlbkGoNDejyPdzwBT7RrKSysuNpWWOhFwxaej0B6 14, 54 -- WnUKcaPJ+SuKMW/xhnyMwzaN0RprAFaYmXw3jxiprDyOngSa2Noi+hJLKz9fdBFA6z/0 14, 54 -- HPGlRoF800tv75WRGuZl2ncek0mSAG1AETKMM4x8VCGfq1tGpwGuolFl4ArFJoJ/VKg/ 14, 54 -- kxVoXaFSgbad4Pv+C2h1/POq1HbsDvW9ru6h62r4B4mtzEv5E3E3i/Bj+zhKbntxXH+Q 14, 54 -- 1P3A== 14, 54 -- X-Gm-Message-State: APjAAAVLEY6ZBiEbkZik1JCpoPmFel1Hm6ajRSBG3i4zM9PGSVLvzltA 14, 54 -- jtwBZkQfRvv0KSfWVFKnCPYPI5nX 14, 54 -- X-Google-Smtp-Source: APXvYqyhg4DcHTpPfxL4P5/4ZL6zXe+l6G7F00tgH9170dcUXCH14LM1g/lcCoyAOGuGzh1AurIhsA== 14, 54 -- X-Received: by 2002:a92:7787:: with SMTP id s129mr1421441ilc.129.1578969314818; 14, 54 -- Mon, 13 Jan 2020 18:35:14 -0800 (PST) 14, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:4432:db4b:adc8:d84b]) 14, 54 -- by smtp.googlemail.com with ESMTPSA id d12sm4485981iln.63.2020.01.13.18.35.14 14, 54 -- for {microscopy-at-microscopy.com} 14, 54 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 14, 54 -- Mon, 13 Jan 2020 18:35:14 -0800 (PST) 14, 54 -- Subject: viaWWW:Staff position CLEM and Bio EM at Monash University, 14, 54 -- Melbourne, Australia 14, 54 -- References: {202001130304.00D34SuG007875-at-microscopy.com} 14, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 14, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 14, 54 -- X-Forwarded-Message-Id: {202001130304.00D34SuG007875-at-microscopy.com} 14, 54 -- Message-ID: {1a370250-39d1-95c5-4302-389e8e1379b9-at-gmail.com} 14, 54 -- Date: Mon, 13 Jan 2020 20:35:14 -0600 14, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) 14, 54 -- Gecko/20100101 Thunderbird/68.3.1 14, 54 -- MIME-Version: 1.0 14, 54 -- In-Reply-To: {202001130304.00D34SuG007875-at-microscopy.com} 14, 54 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 54 -- Content-Language: en-US 14, 54 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: bowmanpond-at-gmail.com Name: Mike Codner
Title-Subject: [Filtered] Light Leakage from Microscope into Digital Camera
Message: While familiarizing myself with a newly purchased Swiftcam 18 MP Microscope Camera, I was having a problem mating it to my LW Scientific Revelation III Binocular Microscope using Swift's 3.0 software and Windows 10. Upon viewing pond water specimens, the background lighting kept changing from white to darker colors and vice-versa. I had made all the adjustments the manual had recommended including exposure, white balance, color settings, and the like. Nothing worked. To make a long story short, the problem resolved itself when I plugged the non-camera binocular with a black rubber stopper after removing the lens. Apparently there had been light leakage from this binocular into the one containing the camera. Just wanted to alert your members since this problem was very frustrating and I could find no helpful information either in the manual or online.
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Email: sichen-at-anl.gov
Name: Si Chen
Organization: Argonne National Laboratory
Title-Subject: [Filtered] Post Doctoral Position - Developing a Cryo-compatible Specimen Prep workflow for Xray and Electron Microscopy -at- Argonne National Laboratory
Message: Position Description
The X-ray Science Division (XSD) of Advanced Photon Source (APS) at Argonne National Laboratory is looking for a Postdoctoral Researcher who will be responsible for developing novel apparatus and methods to enable multi-scale and multi-modality analysis of materials via both electron microscopy and X-ray microscopy at the synchrotron facility. The research aims to deliver a cryo-compatible workflow and supporting techniques to optimize sample preparation for X-ray fluorescence nanoprobe as well as the combined use of multiple analytical platforms. It will also involve software development for image registration across different analytical platforms and correlative data analysis. The successful candidate will work in a multidisciplinary team environment including physicists, chemists, biologists and computer scientist. The candidate will lead the effort in developing the methodology, the deployment at the APS beamlines, as well as applications to materials, biological and environmental sciences. Results will be reported in appropriate forms: publications in refereed journals and oral presentations at meetings, conferences, and seminars. The position will begin in April 2020. It is a one-year position, and renewable for a second year.
Position Requirements
*Ph.D. degree in physics, materials science, engineering, or a related discipline; *Comprehensive knowledge in at least two of the following fields: X-ray physics, X-ray microscopy, electron microscopy, image processing; *Considerable experience with electron microscopy, focused ion beam, synchrotron facility, preferably cryogenic instruments; *Strong data analysis and trouble-shooting skills; *Considerable experience with programming and software tools such as python and git; *Experience with automation, control, and instrument infrastructure is a plus; *Knowledge of biology, chemistry is a plus; *Experience with cryogenic is a plus; *Demonstrated experience working successfully as part of a team in a collaborative and multidisciplinary scientific environment; *Ability to communicate effectively with internal and external collaborators and ability to work in a team environment.
Additional Details and Application Forms can be found at this URL
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Email: whiteto-at-missouri.edu Name: Tommi White
Organization: University of Missouri Electron Microscopy Core
Title-Subject: [Filtered] Cellular & Molecular Electron Microscopy
Message: Hello Microscopy Listserve,
I am taking over a graduate level class entitled "Cellular & Molecular Electron Microscopy" from a retiring faculty member. Would any of you be so kind to share syllabus/lecture materials that I could use in this course? Of course, full credit will be given to the provider of the information! :) Thanks in advance to helping educate the next generation of electron microscopists and light that fire. Tommi Tommi A. White, Ph.D. Director, Electron Microscopy Core Assistant Research Professor, Biochemistry University of Missouri Mail: W117 Veterinary Medicine Bldg 1520 E Rollins St., Columbia, MO 65211 EMC: 573-882-8304 Direct: 573-884-7338 Email: whiteto-at-missouri.edu Web: http://emc.missouri.edu Tweets: -at-MizzouEMC
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There is still plenty of time to apply for Analytical and Quantitative Light Microscopy 2020, held at the Marine Biological Laboratory, Woods Hole, MA, USA. The course will run April 28-May 8, 2020.
Applications are due February 5, 2020. Apply through the course web site at http://www.mbl.edu/aqlm. We especially invite those from underrepresented groups to apply. Reach out if you have any questions!
AQLM is a comprehensive and intensive course in light microscopy for researchers in biology, medicine, and material sciences. This course provides a systematic and in-depth examination of the theory of image formation and application of digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field microscopes, confocal microscopes, and new emerging technologies.
Applications are due February 5, 2020. Apply through the course web site at http://www.mbl.edu/aqlm.
Some refer to it as "microscopy boot camp" because we work hard, but we have a lot of fun in the process!
Course Directors: Peter Kner, University of Georgia Paul Maddox, University of North Carolina at Chapel Hill Wendy Salmon, Whitehead Institute for Biomedical Research Course Laboratory Director: Gary Laevsky, Princeton University
Happy Imaging! Peter, Paul, Wendy and Gary
Best, Wendy
-------------------- Wendy C Salmon, M.A. Light Microscopy Specialist W.M. Keck Facility for Biological Imaging Whitehead Institute for Biomedical Research 455 Main St., Rm 447 Cambridge, MA 02142 e: wsalmon-at-wi.mit.edu w: microscopy.wi.mit.edu
The CCEM just announced that it will be holding its annual summer school on electron microscopy from June 8 - 12, 2020!
A 5-DAY COURSE for users with experience in electron microscopy, on the fundamentals of aberration-corrected imaging, electron energy loss spectroscopy, energy dispersive X-ray spectroscopy, electron tomography, ultimate physical limits (beam damage and resolution), DPC microscopy and the use of aberration-corrected electron microscopes. The aim is to provide students a device in solving characterization problems with the help of experts. The course will include lectures given by experts in the use of the technique and experts in electron optics, alignment and optimization of electron microscopes and EELS spectrometers. Students will have plenty of opportunities for hands-on training on the alignment and operation of the electron microscopes with the experts from the microscope and spectrometer companies. Two FEI Titan microscopes with correctors and monochromators (GIF Quantum and K2) will be used for training, as well as a Talos F200X. Several hands-on data processing sessions are also organised.
DATE: June 8 - 12, 2020
COST: All meals and course notes are included in the registration fee ranging from $700.CDN/full-time students to $2000.CDN/industry researchers. Accommodation will be separate and the responsibility of attendees (see full details on registration form).
REGISTRATION: Register online by January 31, 2020: https://ccem.mcmaster.ca/ccem-summer-school-2020
--† Dr. Andreas Korinek
Manager
Canadian Centre for Electron Microscopy McMaster University 1280 Main Street West, Hamilton ON Canada, L8S 4M1 phone: +1 905-525-9140 ext 20400
==============================Original Headers============================== 10, 24 -- From korinek-at-mcmaster.ca Tue Jan 21 16:06:23 2020 10, 24 -- Received: from FHSHC4H16-1.csu.mcmaster.ca (fhshc4h16-1.csu.mcmaster.ca [130.113.22.3]) 10, 24 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 00LM6NCV011274 10, 24 -- for {microscopy-at-microscopy.com} ; Tue, 21 Jan 2020 16:06:23 -0600 10, 24 -- Received: from FHSDB4H16-2.csu.mcmaster.ca ([fe80::74a1:2461:9f81:dcf6]) by 10, 24 -- FHSHC4H16-1.csu.mcmaster.ca ([2002:8271:1603::8271:1603]) with mapi id 10, 24 -- 14.03.0468.000; Tue, 21 Jan 2020 16:04:23 -0500 10, 24 -- From: "Korinek, Andreas" {korinek-at-mcmaster.ca} 10, 24 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 10, 24 -- Subject: CCEM TEM Summer School 2020 10, 24 -- Thread-Topic: CCEM TEM Summer School 2020 10, 24 -- Thread-Index: AdXQni4YUMlvldb5RU+8pIUNlcb1PQ== 10, 24 -- Date: Tue, 21 Jan 2020 21:04:23 +0000 10, 24 -- Message-ID: {4CB3BB07DE33A340B9F8F8DAFE73D7A50128A63437-at-FHSDB4H16-2.csu.mcmaster.ca} 10, 24 -- Accept-Language: en-CA, en-US 10, 24 -- Content-Language: en-US 10, 24 -- X-MS-Has-Attach: 10, 24 -- X-MS-TNEF-Correlator: 10, 24 -- x-originating-ip: [130.113.22.232] 10, 24 -- x-tm-snts-smtp: 1EFC72829D7B8CA3BCC17918393EA6E5DF4299295DDE40C98E96DACBA1D4A6742 10, 24 -- Content-Type: text/plain; charset="iso-8859-1" 10, 24 -- MIME-Version: 1.0 10, 24 -- Content-Transfer-Encoding: 8bit 10, 24 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 00LM6NCV011274 ==============================End of - Headers==============================
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Email: bassimn-at-mcmaster.ca
Name: Nabil Bassim
Organization: McMaster University
Title-Subject: [Filtered] Postdoc/Research Associate Position Available - McMaster University
Message: The Bassim research group is seeking a postdoc or research associate who will work on a varied portfolio of projects, including developing novel 2-D van der Waals heterostructures, catalysts, and metamaterials. The chosen candidate would also be encouraged to develop their own microscopy techniques using the CCEM infrastructure. The research projects involve strong collaboration with synthesis teams, and proven ability to work in interdisciplinary groups should be demonstrated. Teaching, communication, and presentation skills are part of a successful post-doctoral or research associate position, and academic/industrial/governmental experience in these areas should be clearly detailed. Day-to-day supervisory tasks with graduate students in the CCEM and within the Bassim research group would be expected, and examples of administrative, project management, and supervisory skills would be a plus. Requirements for the role include: MANDATORY - A Ph.D. in Materials Science or Physics - Experience in aberration-corrected imaging and electron energy loss spectroscopy - A strong track record in publishing TEM-related research
DESIRED - Interest in mentoring graduate students - Experience with in-situ techniques - Capability to perform image simulations and scattering theory - Sample preparation capabilities using FIB Pay will range based on level of experience and track record. The appointment is for 1 year, renewable up to 3 years.
Located at McMaster University in Hamilton, Ontario, the work would be performed at the Canadian Centre for Electron Microscopy (CCEM), which is home to 10 technical staff and hundreds of users with many diverse interests. The CCEM has a very elaborate suite of advanced instrumentation with plans for future expansion. Hamilton is a lovely city in Southern Ontario, located midway between Toronto and Niagara Falls with a mild (by Canadian standards) winter.
If you are interested, please contact bassimn at mcmaster.ca with a CV and several references.
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Message: We are looking to extend the life of our WinNT XL30 ESEM with an upgrade. It seems FEI no longer offers an upgrade but there are apparently several options available through third party vendors.
I would appreciate hearing directly from anyone willing to share their experiences who have gone the third party route.
Also if you're contemplating decommissioning a Win 2000 XL30 in the near future and are looking for someone to take it off your hands, please contact me.
Thanks, Bill Mushock wim5-at-lehigh.edu
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----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: xbren-at-uw.edu Name: Shirley
Organization: UW
Title-Subject: [Filtered] UCT controller Message: Hi, Can someone give me any ideas? One of my lab's ultra-cut microtome(Leica UCT) controller doesn't work any more, and we found that the power unit is down. We reached Leica, but they said they don't have this model and don't do repair on this model. The microtome is good, but it's only the controller that doesn't work. Do you know where to repair/replace this kind of controller?
Many thanks!
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Email: jhyun-at-gatan.com Name: John Hyun
Organization: Gatan, Inc.
Title-Subject: [Filtered] EELS & EFTEM Analysis Training School April 2020
Message: EELS & EFTEM Analysis Training School April 2020 April 21 ñ 24, 2020 Gatan R&D Headquarters, Pleasanton, CA
We invite you to our upcoming course to learn best practices to set up your EELS hardware, optimize experimental protocols, then capture, and extract the maximum amount of compositional and chemical information from your TEM samples. Topics include:
ï Fundamentals of EELS and energy-filtered imaging in TEM ï Principles of operation of EFTEM and EELS systems ï Optimization of EFTEM and EELS data acquisition ï Quantification of elemental composition ï Other information provided by EFTEM/EELS and how best to extract it ï Use of EELS signals to form maps of elemental and chemical composition ï EFTEM and STEM EELS spectrum imaging techniques ï Identification of material phases via EELS fine structure mapping ï Applications to biological and physical science specimens
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Title-Subject: [Filtered] pathological report for mouse kidney EM
Message: Hi, Do you know any lab that could do TEM of mouse kidneys, including a pathology report?
Closer to NYC area would be a plus.
Thank you!
AMALIA Hilda Amalia Pasolli, Ph.D. Director and Research Associate Professor Electron Microscopy Resource Center RRB 120F The Rockefeller University 1230 York Avenue, Box 230 New York, NY 10065 Phone 212 327 8325
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Message: Location: Peter MacCallum Cancer Centre, Melbourne Australia
Peter MacCallum Cancer Centre is seeking an experienced and highly motivated scientist to join the Centre for Advanced Histology and Microscopy (CAHM). CAHM encompasses research histology, optical and electron microscopy and provides researchers with advice, tuition and technical expertise.
The applicant will have a passion for optical microscopy and in-depth understanding of the operation of optical microscopes, including confocal, super resolution, and multiphoton microscopy. The applicant will enjoy interacting with researchers and assisting them with their imaging needs.
The position is initially for a fixed term of 12 months with the opportunity for future extension.
Contact Person: Sarah Ellis Contact Number: +613 8559 7822 Contact Email: sarah.ellis-at-petermac.org Closing Date: 08 April 2020
To see the position description and apply go to https://petermac.mercury.com.au/ViewPosition.aspx?id=heS2osrVG/U=&jbc=ere
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X-from: Lee Cohen-Gould {lcgould-at-med.cornell.edu}
Hi Amalia- Happy New Year. What kind of pathology do you expect?† In the cortex or medulla?† Glomeruli, tubules or calex? I can send you a protocol for mouse kidney, or we can do it here, but we don't do the pathology analysis.† You should speak to the people in your animal facility. They should be able to either do it or refer you to someone, perhaps at the Animal Medical Center. I could put you in touch with our clinical EM facility here. They specialize in human kidney pathology. Let me know what you need. Best, Lee
---------------------------------------------------------------------------------------------------- *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* Wednesday, January 29, 2020 10:14 AM *To:* Lee Cohen-Gould {lcgould-at-med.cornell.edu} *Subject:* [EXTERNAL] [Microscopy] viaWWW: pathological report for mouse kidney EM
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Title-Subject: [Filtered] pathological report for mouse kidney EM
Message: Hi, Do you know any lab that could do TEM of mouse kidneys, including a pathology report?
Closer to NYC area would be a plus.
Thank you!
AMALIA Hilda Amalia Pasolli, Ph.D. Director and Research Associate Professor Electron Microscopy Resource Center RRB 120F The Rockefeller University 1230 York Avenue, Box 230 New York, NY 10065 Phone 212 327 8325
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Email: harry.murray-at-dfo-mpo.gc.ca
Name: Harry M. Murray
Organization: Fisheries and Oceans Canada
Title-Subject: [Filtered] Error Code for SU1510
Message: We purchased a Hitachi SU1510 SEM about 4 years ago and have had some good luck with it but recently our roughing pump has gone down and while now functionalÖ.we still canít get our scope to boot up. We keep getting the 7313 error code indicating a pump issue still exists. We are wondering if anybody might have experienced such an error in the past and thus might know a fix that would not require a Hitachi engineer and the associated cost.
Thanks in advance,
Harry
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Title-Subject: [Filtered] M&M 2020 - Important Submission Info!
Message: The M&M 2020 submission portal is OPEN. The submission DEADLINE is February 21, 2020; 11:59 PM U.S. Pacific Time. Click on the link below to access the online portal: {b} https://www.abstractscorecard.com/cfp/submit/login.asp?EventKey=AKMWPRYI {b}
Please note! M&M is using a NEW submission system this year, so the form structure and process are different from recent previous years. Visit the M&M 2020 website for important details and submission information! https://www.microscopy.org/MandM/2020/program/submit.cfm
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Organization: Royal Microscopical Society
Title-Subject: [Filtered] European Microscopy Congress 2020 - Abstract Submission Deadline 1 March 2020
Message: The European Microscopy Congress 2020 (emc2020) in Copenhagen is being hosted by SCANDEM, and organised by the Royal Microscopical Society under the auspices of the EMS and IFSM. The European Microscopy Congress is known for being the largest European stage for cross-disciplinary research. The broad scientific programme will welcome contributions from all over the world, showcasing the latest research in life sciences, physical sciences and engineering across all microscopy and imaging techniques. Over 30 conference sessions, an exhibition with more than 100 companies represented and a brilliant selection of features such as pre-event workshops and social networking events.
Oral and Poster abstract submission is currently open for the European Microscopy Congress 2020. Go to www.emc2020.eu/abstract-submission where you can create an account and then submit your abstract. The deadline for submitting your abstract is 1 MARCH 2020.
We are accepting abstracts for the following symposia: ï Life Sciences: Applications ï Life Sciences: Tools & Techniques ï Physical Sciences: Applications ï Physical Sciences: Tools & Techniques ï Data Handling and Analysis
To see a full list of the individual sessions and their descriptions, the provisional programme including the fantastic line up of invited speakers, please visit: https://www.emc2020.eu/conference/conference-sessions.html. You can also view the confirmed plenary speakers here: https://www.emc2020.eu/conference/plenary-speakers.html.
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Electron source with beam current of a few uA wouldn't exactly be from SEM or EBL domain, but.... I would suggest to try speaking to: Kimball Physics https://www.kimballphysics.com/
No connection other then a satisfied customer here.
Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 2/6/2020 5:25 AM, koeck-at-kth.se wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } ​ } } I'm looking for an electron source, used or new, that can produce a beam with the following characteristics: } } energy: 1 keV or less } current: in the order of a few microAmps } beam waist diameter: 1 micrometer or less } } I'm thinking of a gun and a single condenser lens if that is possible. Small is good. } } Something used in a SEM, in e-beam lithography or electron evaporation might be a possibility. } } Can anyone recommend something? } } All the best, } } Philip } } } ==============================Original Headers============================== } 9, 44 -- From koeck-at-kth.se Thu Feb 6 04:24:56 2020 } 9, 44 -- Received: from smtp-3.sys.kth.se (smtp-3.sys.kth.se [130.237.48.192]) } 9, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 016AOt2x016207 } 9, 44 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2020 04:24:56 -0600 } 9, 44 -- Received: from smtp-3.sys.kth.se (localhost.localdomain [127.0.0.1]) } 9, 44 -- by smtp-3.sys.kth.se (Postfix) with ESMTP id 70CA4A197 } 9, 44 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2020 10:21:32 +0100 (CET) } 9, 44 -- X-Virus-Scanned: by amavisd-new at kth.se } 9, 44 -- Received: from smtp-3.sys.kth.se ([127.0.0.1]) } 9, 44 -- by smtp-3.sys.kth.se (smtp-3.sys.kth.se [127.0.0.1]) (amavisd-new, port 10024) } 9, 44 -- with LMTP id Ls36YmGqaqdO for {microscopy-at-microscopy.com} ; } 9, 44 -- Thu, 6 Feb 2020 10:21:31 +0100 (CET) } 9, 44 -- Received: from exdb02.ug.kth.se (exdb02.ug.kth.se [192.168.32.112]) } 9, 44 -- by smtp-3.sys.kth.se (Postfix) with ESMTPS id BE9BEA0CD } 9, 44 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2020 10:21:31 +0100 (CET) } 9, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=kth.se; s=default; } 9, 44 -- t=1580980891; bh=Wy1rFUq+dDYbR/0UIILMHN9U2veLzq4h3W6GL8RulTA=; } 9, 44 -- h=From:To:Subject:Date; } 9, 44 -- b=XB6XzMXCuDFNZXxVSisrZJ/j8aThW2472gE9kX9OxpPEdAGFzldnLl9P7+OR3LVV/ } 9, 44 -- QTFX3Qxbqaez1ZgIYOPAC7c5/rd2/IFF7vGNUu491N4NPfpMiNTSQXezh/ANjXp2hd } 9, 44 -- vHtSMvVoBEwhPe3hP34MpsPL0vfvJ9qGgv1R0Z0Y= } 9, 44 -- Received: from exdb01.ug.kth.se (192.168.32.111) by exdb02.ug.kth.se } 9, 44 -- (192.168.32.112) with Microsoft SMTP Server (TLS) id 15.0.1473.3; Thu, 6 Feb } 9, 44 -- 2020 10:21:31 +0100 } 9, 44 -- Received: from exdb01.ug.kth.se ([192.168.32.111]) by exdb01.ug.kth.se } 9, 44 -- ([192.168.32.111]) with mapi id 15.00.1473.005; Thu, 6 Feb 2020 10:21:30 } 9, 44 -- +0100 } 9, 44 -- From: =?utf-8?B?UGhpbGlwIEvDtmNr?= {koeck-at-kth.se} } 9, 44 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 9, 44 -- Subject: electron source } 9, 44 -- Thread-Topic: electron source } 9, 44 -- Thread-Index: AQHV3M3i8bnQRRu9D0Sdsm6FG8iYOw== } 9, 44 -- Date: Thu, 6 Feb 2020 09:21:30 +0000 } 9, 44 -- Message-ID: {1580980891958.41450-at-kth.se} } 9, 44 -- Accept-Language: sv-SE, en-US } 9, 44 -- Content-Language: sv-SE } 9, 44 -- X-MS-Has-Attach: } 9, 44 -- X-MS-TNEF-Correlator: } 9, 44 -- x-ms-exchange-transport-fromentityheader: Hosted } 9, 44 -- x-originating-ip: [46.59.23.142] } 9, 44 -- Content-Type: text/plain; charset="utf-8" } 9, 44 -- MIME-Version: 1.0 } 9, 44 -- Content-Transfer-Encoding: 8bit } 9, 44 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id 016AOt2x016207 } ==============================End of - Headers============================== }
Electron source with beam current of a few uA wouldn't exactly be from SEM or EBL domain, but.... I would suggest to try speaking to: Kimball Physics https://www.kimballphysics.com/
No connection other then a satisfied customer here.
Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 2/6/2020 5:25 AM, koeck-at-kth.se wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } ​ } } I'm looking for an electron source, used or new, that can produce a beam with the following characteristics: } } energy: 1 keV or less } current: in the order of a few microAmps } beam waist diameter: 1 micrometer or less } } I'm thinking of a gun and a single condenser lens if that is possible. Small is good. } } Something used in a SEM, in e-beam lithography or electron evaporation might be a possibility. } } Can anyone recommend something? } } All the best, } } Philip } } } ==============================Original Headers============================== } 9, 44 -- From koeck-at-kth.se Thu Feb 6 04:24:56 2020 } 9, 44 -- Received: from smtp-3.sys.kth.se (smtp-3.sys.kth.se [130.237.48.192]) } 9, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 016AOt2x016207 } 9, 44 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2020 04:24:56 -0600 } 9, 44 -- Received: from smtp-3.sys.kth.se (localhost.localdomain [127.0.0.1]) } 9, 44 -- by smtp-3.sys.kth.se (Postfix) with ESMTP id 70CA4A197 } 9, 44 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2020 10:21:32 +0100 (CET) } 9, 44 -- X-Virus-Scanned: by amavisd-new at kth.se } 9, 44 -- Received: from smtp-3.sys.kth.se ([127.0.0.1]) } 9, 44 -- by smtp-3.sys.kth.se (smtp-3.sys.kth.se [127.0.0.1]) (amavisd-new, port 10024) } 9, 44 -- with LMTP id Ls36YmGqaqdO for {microscopy-at-microscopy.com} ; } 9, 44 -- Thu, 6 Feb 2020 10:21:31 +0100 (CET) } 9, 44 -- Received: from exdb02.ug.kth.se (exdb02.ug.kth.se [192.168.32.112]) } 9, 44 -- by smtp-3.sys.kth.se (Postfix) with ESMTPS id BE9BEA0CD } 9, 44 -- for {microscopy-at-microscopy.com} ; Thu, 6 Feb 2020 10:21:31 +0100 (CET) } 9, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=kth.se; s=default; } 9, 44 -- t=1580980891; bh=Wy1rFUq+dDYbR/0UIILMHN9U2veLzq4h3W6GL8RulTA=; } 9, 44 -- h=From:To:Subject:Date; } 9, 44 -- b=XB6XzMXCuDFNZXxVSisrZJ/j8aThW2472gE9kX9OxpPEdAGFzldnLl9P7+OR3LVV/ } 9, 44 -- QTFX3Qxbqaez1ZgIYOPAC7c5/rd2/IFF7vGNUu491N4NPfpMiNTSQXezh/ANjXp2hd } 9, 44 -- vHtSMvVoBEwhPe3hP34MpsPL0vfvJ9qGgv1R0Z0Y= } 9, 44 -- Received: from exdb01.ug.kth.se (192.168.32.111) by exdb02.ug.kth.se } 9, 44 -- (192.168.32.112) with Microsoft SMTP Server (TLS) id 15.0.1473.3; Thu, 6 Feb } 9, 44 -- 2020 10:21:31 +0100 } 9, 44 -- Received: from exdb01.ug.kth.se ([192.168.32.111]) by exdb01.ug.kth.se } 9, 44 -- ([192.168.32.111]) with mapi id 15.00.1473.005; Thu, 6 Feb 2020 10:21:30 } 9, 44 -- +0100 } 9, 44 -- From: =?utf-8?B?UGhpbGlwIEvDtmNr?= {koeck-at-kth.se} } 9, 44 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 9, 44 -- Subject: electron source } 9, 44 -- Thread-Topic: electron source } 9, 44 -- Thread-Index: AQHV3M3i8bnQRRu9D0Sdsm6FG8iYOw== } 9, 44 -- Date: Thu, 6 Feb 2020 09:21:30 +0000 } 9, 44 -- Message-ID: {1580980891958.41450-at-kth.se} } 9, 44 -- Accept-Language: sv-SE, en-US } 9, 44 -- Content-Language: sv-SE } 9, 44 -- X-MS-Has-Attach: } 9, 44 -- X-MS-TNEF-Correlator: } 9, 44 -- x-ms-exchange-transport-fromentityheader: Hosted } 9, 44 -- x-originating-ip: [46.59.23.142] } 9, 44 -- Content-Type: text/plain; charset="utf-8" } 9, 44 -- MIME-Version: 1.0 } 9, 44 -- Content-Transfer-Encoding: 8bit } 9, 44 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id 016AOt2x016207 } ==============================End of - Headers============================== }
*********************************************************************************** Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. *********************************************************************************** Name: Aubrey Penn Email: anpenn-at-ncsu.edu
Avoiding surface damage during FIB sample preparation is fairly straight-forward: prior to ever exposing sample ion beam it should be coated with sufficient thickness (~100nm optimal, but } 30nm is a minimal requirement) of some protective layer that will "absorb" ion beam damage.
Following coatings may typically be used for such protective layer, depending on nature of the sample: (a) e-beam deposition of C, Pt, Mo, W, SiOx, etc...; (b) evaporated carbon or metal; (c) sputter coating by Au, Au/Pd, TiO, Cr, Ir, etc...; (d) conductive polymer coating by spinning or ultrasonic nozzle dispensing; (e) ink coating (i.e. "Sharpie trick), etc...
For atomic resolution TEM amorphous layer on the sides of the lamella would also need to be cleaned, but that is already another question.
Happy sample prepping :) Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 2/7/2020 11:52 AM, oshel1pe-at-cmich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely not a member the listserver, and } **any reply should go directly to the poster** as well as to the list. } Using the "reply" function in your email does *not* send your answer to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } Name: Aubrey Penn } Email: anpenn-at-ncsu.edu } Subject: FIB sample preparation } Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging? } } I'm not a FIB expert, but there are plenty on the microscopy list. There will probably be other people who would like to know the answer to this question, too, so please reply both directly to Penn and to the list. } } ------------- } Philip Oshel } Microscopy Society of America } Ask a Microscopist } www(dot)microscopy(dot)org/resources/ask(dot)cfm } } } } ==============================Original Headers============================== } 5, 73 -- From oshel1pe-at-cmich.edu Fri Feb 7 10:52:13 2020 } 5, 73 -- Received: from NAM04-BN3-obe.outbound.protection.outlook.com (mail-eopbgr680123.outbound.protection.outlook.com [40.107.68.123]) } 5, 73 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 017GqDnD026736 } 5, 73 -- for {microscopy-at-microscopy.com} ; Fri, 7 Feb 2020 10:52:13 -0600 } 5, 73 -- ARC-Seal: i=1; a=rsa-sha256; s=arcselector9901; d=microsoft.com; cv=none; } 5, 73 -- b=da/U+R0sXKlzOTSmd81mndzAIiEF6CYYfnreGverm4M7nWp/g+xp2MHBfLbj7TsJNWtztnrUJ7s3NDz8mgKWbCxwmIiNUX6C/gzQSAjNN9N1+B0zHGww5XQkdiaky/nUnwFfaO/y2QEqLydaiP4dYELc6udXby/GdEeHRAAafRsLSM3PVAXxBOOXl7ZOGWyHK0s7D3IoGjnQlwJ5S+RYspATzHPMCJN9/j+Yk4yp4INPHLk3y+l0fUrSHH6Y4U0YG/fGD9pwkTcnPYsbgN0HDmKATDoU5666L+QdMSO1VehIRShsB19O+2sToE9bPC5HsIkH65LOCJAWxgiU/p3F6A== } 5, 73 -- ARC-Message-Signature: i=1; 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Avoiding surface damage during FIB sample preparation is fairly straight-forward: prior to ever exposing sample ion beam it should be coated with sufficient thickness (~100nm optimal, but } 30nm is a minimal requirement) of some protective layer that will "absorb" ion beam damage.
Following coatings may typically be used for such protective layer, depending on nature of the sample: (a) e-beam deposition of C, Pt, Mo, W, SiOx, etc...; (b) evaporated carbon or metal; (c) sputter coating by Au, Au/Pd, TiO, Cr, Ir, etc...; (d) conductive polymer coating by spinning or ultrasonic nozzle dispensing; (e) ink coating (i.e. "Sharpie trick), etc...
For atomic resolution TEM amorphous layer on the sides of the lamella would also need to be cleaned, but that is already another question.
Happy sample prepping :) Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 2/7/2020 11:52 AM, oshel1pe-at-cmich.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely not a member the listserver, and } **any reply should go directly to the poster** as well as to the list. } Using the "reply" function in your email does *not* send your answer to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } Name: Aubrey Penn } Email: anpenn-at-ncsu.edu } Subject: FIB sample preparation } Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging? } } I'm not a FIB expert, but there are plenty on the microscopy list. There will probably be other people who would like to know the answer to this question, too, so please reply both directly to Penn and to the list. } } ------------- } Philip Oshel } Microscopy Society of America } Ask a Microscopist } www(dot)microscopy(dot)org/resources/ask(dot)cfm } } } } ==============================Original Headers============================== } 5, 73 -- From oshel1pe-at-cmich.edu Fri Feb 7 10:52:13 2020 } 5, 73 -- Received: from NAM04-BN3-obe.outbound.protection.outlook.com (mail-eopbgr680123.outbound.protection.outlook.com [40.107.68.123]) } 5, 73 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 017GqDnD026736 } 5, 73 -- for {microscopy-at-microscopy.com} ; Fri, 7 Feb 2020 10:52:13 -0600 } 5, 73 -- ARC-Seal: i=1; a=rsa-sha256; s=arcselector9901; d=microsoft.com; cv=none; } 5, 73 -- b=da/U+R0sXKlzOTSmd81mndzAIiEF6CYYfnreGverm4M7nWp/g+xp2MHBfLbj7TsJNWtztnrUJ7s3NDz8mgKWbCxwmIiNUX6C/gzQSAjNN9N1+B0zHGww5XQkdiaky/nUnwFfaO/y2QEqLydaiP4dYELc6udXby/GdEeHRAAafRsLSM3PVAXxBOOXl7ZOGWyHK0s7D3IoGjnQlwJ5S+RYspATzHPMCJN9/j+Yk4yp4INPHLk3y+l0fUrSHH6Y4U0YG/fGD9pwkTcnPYsbgN0HDmKATDoU5666L+QdMSO1VehIRShsB19O+2sToE9bPC5HsIkH65LOCJAWxgiU/p3F6A== } 5, 73 -- ARC-Message-Signature: i=1; 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Email: elena.belluso-at-unito.it
Name: Elena Belluso
Organization: University of Torino - I
Title-Subject: [Filtered] session 13d ìAirborne particles and fibers: characteristics, sources, toxicology, and impacts on ecosystems and human healthî, Goldschmidt 2020
Message: Dear Colleague, We are pleased to invite you to submit an abstract for an oral presentation (or a poster) to the session 13d: AIRBORNE PARTICLES AND FIBERS: CHARACTERISTICS, SOURCES, TOXICOLOGY, AND IMPACTS ON ECOSYSTEMS AND HUMAN HEALTHî (Theme 13 https://goldschmidt.info/2020/program/programViewThemes) at Goldschmidt 2020, the international conference on geochemistry and related subjects, scheduled from 21 to 26 June 2020 in Honolulu, Hawaii.
The key-note speaker of the session is Reto GierÈ (University of Pennsylvania): ìMineralogy, Chemistry, and Toxicology of Particulate Emissions from Combustion Processesî.
Convenors: Elena Belluso (Universit‡ degli Studi di Torino, Torino, Italy), Janice Brahney (Utah State University, Logan, UT, USA), Francesco Di Benedetto (Universit‡ degli Studi di Firenze, Firenze, Italy), Peggy O'Day (University of California, Merced, CA, USA)
Note that the abstract deadline is February, 14, 2020 (https://goldschmidt.info/2020/abstracts)
We look forward to seeing you in Honolulu in June!
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X-from: Richard E. Edelmann {Edelmare-at-miamioh.edu}
Andrew,
There are really only three factors:
(1) The film. Is it old or bad? Has something changed in the development? (temp or developer aged etc.)
(2) Is the Sens seeting on the scope still correct? Which you've chanegd all the way up to 20? That is very high.
Finally I am betting it is:
(3) Is the beam current being correct read by the scope? As Page-1 of the CRT display shows the Curr Dens as received on the screen. Does that adjust when you adjust the brightness control?
--} Secondly, there is an adjustment that is made to the Curr Dens reading when the focusing screen is in the beam path vs when it is out. Does the vale look reasonable in vs out? --} I have had both bad signal from the main viewing screen and the adjustment from the focusing screen in vs out.
On 19 Dec 2019 at 8:52, microscopy.listserver-at-gmail.c wrote:
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Recently, the film started to come out completely } under-exposed. It was discovered that the film exposure setting had } changed to 20 out of 20. Since then, we have tried many different } parameters and have had no positive results as far as useful images. } After adjusting the FSE we have an image again, but now we are } struggling to obtain a scale of contrast. Despite being in-focus and } displaying layers of contrast on screen - the film continues to } develop in a more black-and-white situation. } } We have gone through changing accelerating voltage, new film, new } developing chem, exposure times, the full range of FSE, aperture } adjustments and we are still hitting a wall. Has anyone dealt with } this or (preferably) know how to resolve this issue? 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Richard E. Edelmann, Ph.D., Director Center for Advanced Microscopy & Imaging 9C Upham Hall Miami University, Oxford, OH 45056 Ph: 513.529.5712 Fax: 513.529.4243 E-mail: edelmare-at-miamioh.edu http://www.cami.muohio.edu
Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging?
I'm going to answer this in several parts.
You don't say what the material or the substrate is. I assume that it is a semiconductor. If it is and the sample doesn't have to be site-specific, then the best way to prepare a 20 nm thick epitaxial film and avoid Ga damage is not to use the FIB at all.
1. The absolute best way to prepare your sample with no amorphous damage at all is the Small Angle Cleavage Technique originally developed by John McCaffrey and then modified quite a bit by John and myself. A major disadvantage of this technique is that there is no amorphous damage and so it can be difficult to focus and stigmate in a field emission TEM. I will sometimes put a thin layer of ca carbon on the top surface just to have something amorphous that I can use in the FFT to help focus and stigmata. If you want, I can send you a detailed presentation on how to do the technique. Just send a request to my work email: (scott-dot-d-dot-walck2-dot-ctr-at-mail-dot-mil).
2. The next best way of preparing your sample would be low angle, low energy Ar ion milling developed by Arpad Barna. Now, all the manufacturers of ion mills have low angle, low energy capabilities. Arpad has several publications out that show the amorphous damage at different energies and angles.
3. OK, so you have a FIB and you don't want to learn how to make samples the old fashioned way. You have to protect the top layer from ion damage before putting on the ion beam Pt (or W) protective layer. You can put it on by e-beam deposition. All you need is 200-250 nm thick to stop and ions from hitting the top surface. Just don't let the ion beam hit the surface with too high of a current or for very long (minimize the dosage to the surface or you will erode the protective layer.) If your sample is not site specific and you don't want to have a high Z material next to your very thin layer, you should consider putting carbon down. Any easy way to do this is to use a Sharpie(R) pen and coat the surface before you put it in the FIB. This works, but can lead to open areas and what looks a bit stringy. A better way is to use a carbon coater to put a uniform layer down. We have a Leica ACE 600 and I just measured the thickness of carbon using a double carbon thread and using it all up. It gave a thickness of 390 nm, which is plenty. At any rate, putting the carbon layer next to your thin layer gives a better contrast between the layer of interest and the protective top layer to see it better. The high Z of Pt or W can make it difficult to see a very thin layer at the top of the surface of your sample.
When preparing the sample by FIB, you will have an amorphous damage layer on top and bottom with a thickness that is dependent on the energy of the beam that you last use. The newest FIBs are capable of "polishing" with a 2 keV (or lower) Ga beam to minimize the damage layer. It is done by exposing the two surfaces at an angle of 3∞. Samples prepared this way are pretty good, but remember, there is still an amorphous layer on both the top and bottom surfaces.
There are also other ways of removing FIB damage from the surfaces of FIB lamella using Ar ion milling. In decreasing order of cost:
-E.A. Fischione makes the NanoMill and the PicoMill, which use a scanning Ar beam to polish the surfaces with low energy Ar. They have presentations and product information on these tools.
-Several manufacturers of ion mills, specifically Gatan and Technoorg Linda of recipes for removing damage using their tools. You should contact them for papers and presentations on how to do that.
-Another technique that I patented while working at South Bay Technology is the Plasma Trimming method, which uses the shape of the FIB sample and a bias to create a field that causes ions from a plasma to polish the two surface of the sample. The plasma was generating using Ar gas in a plasma cleaner. This patent is now owned by Ted Pella, Inc. I can provide a Plasma Trimming presentation upon request. (scott-dot-d-dot-walck2-dot-ctr-at-mail-dot-mil)
BTW, you can estimate the amount of damage and depth for different ions, energies, and angles by using SRIM and TRIM calculations. See www.srim.org for more information and to download the software and manual.
I hope that this helps. I'm sure it is more than you need.
-Scott
-----Original Message----- X-from: oshel1pe-at-cmich.edu Sent: Friday, February 7, 2020 11:55 AM To: s.walck-at-comcast.net
**************************************************************************** ******* Forwarded from "Ask a Microscopist" Please remember that the person asking the question is likely not a member the listserver, and **any reply should go directly to the poster** as well as to the list. Using the "reply" function in your email does *not* send your answer to the person asking the question. Please copy their email address from their question. **************************************************************************** ******* Name: Aubrey Penn Email: anpenn-at-ncsu.edu
Hello Aubrey,
Good to see you on the listserv!
In regards to your question, Valery and Scott gave you some good advice. I've always referred students to the following paper: https://www.sciencedirect.com/science/article/abs/pii/S030439911200006X I can give you a copy the next time I see you.
There are a lot of ways to skin a cat, as they say, but it can be challenging to get good FIB samples if you're used to the results generated by tripod polishing and low energy, angle ion milling. The best results I've seen are from FIB samples prepared in a similar manner as in the paper above, followed by a cleanup in a low energy ion mill (Fischione, Gatan, Technoorg-Linda...all are fine). However, the ion mill cleanup is not magic. You can start to etch your liftout if the angle and voltages are not ideal, or if you mill for too long to compensate for a thicker liftout.
Depending on the materials, you may also be able to dip the liftout in a dilute acid or base to thin the liftout, or use a flash electropolishing setup like the one they have at PNNL to reduce the liftout thickness and remove damage layers. Regardless of what post-FIB step you use, you need to do your best to create a thin, flat liftout with a minimal amount of sputter redep in the first place, and that's where that paper gives some valuable tips.
Good luck, Chris
On Fri, Feb 7, 2020 at 10:52 AM {oshel1pe-at-cmich.edu} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } *********************************************************************************** } Forwarded from "Ask a Microscopist" } Please remember that the person asking the question is likely not a member the listserver, and } **any reply should go directly to the poster** as well as to the list. } Using the "reply" function in your email does *not* send your answer to the person asking the question. } Please copy their email address from their question. } *********************************************************************************** } Name: Aubrey Penn } Email: anpenn-at-ncsu.edu } Subject: FIB sample preparation } Your Question: What are the best ways to avoid damaging or outright destroying an epitaxial thin film (~20nm thick) film using Ga ion FIB for atomic resolution STEM imaging? } } I'm not a FIB expert, but there are plenty on the microscopy list. There will probably be other people who would like to know the answer to this question, too, so please reply both directly to Penn and to the list. } } ------------- } Philip Oshel } Microscopy Society of America } Ask a Microscopist } www(dot)microscopy(dot)org/resources/ask(dot)cfm } } } } ==============================Original Headers============================== } 5, 73 -- From oshel1pe-at-cmich.edu Fri Feb 7 10:52:13 2020 } 5, 73 -- Received: from NAM04-BN3-obe.outbound.protection.outlook.com (mail-eopbgr680123.outbound.protection.outlook.com [40.107.68.123]) } 5, 73 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 017GqDnD026736 } 5, 73 -- for {microscopy-at-microscopy.com} ; Fri, 7 Feb 2020 10:52:13 -0600 } 5, 73 -- ARC-Seal: i=1; a=rsa-sha256; s=arcselector9901; d=microsoft.com; cv=none; } 5, 73 -- b=da/U+R0sXKlzOTSmd81mndzAIiEF6CYYfnreGverm4M7nWp/g+xp2MHBfLbj7TsJNWtztnrUJ7s3NDz8mgKWbCxwmIiNUX6C/gzQSAjNN9N1+B0zHGww5XQkdiaky/nUnwFfaO/y2QEqLydaiP4dYELc6udXby/GdEeHRAAafRsLSM3PVAXxBOOXl7ZOGWyHK0s7D3IoGjnQlwJ5S+RYspATzHPMCJN9/j+Yk4yp4INPHLk3y+l0fUrSHH6Y4U0YG/fGD9pwkTcnPYsbgN0HDmKATDoU5666L+QdMSO1VehIRShsB19O+2sToE9bPC5HsIkH65LOCJAWxgiU/p3F6A== } 5, 73 -- ARC-Message-Signature: i=1; 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-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
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Title-Subject: [Filtered] We are hiring! Electron Microscopist / Imaging Specialist
Message: Hello! Sivananthan Laboratories, Inc. is a high-tech business incubator focused on promoting economic growth in Illinois and the United States through fostering cutting-edge, fundamental research and development. We work on R&D for AI, infrared detectors, image processing, solar energy, and more. We are currently looking for a successful candidate who is skilled in all aspects on scanning electron microscopy (SEM), transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM) with experience in image simulation and state-of-the-art processing and analytics packages. If you are interested, please go to this link (http://sivananthanlabs.us/employment/) and apply for this position!
If you have any questions, feel free to email me at mrajasingham-at-sivananthanlabs.us
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Email: xbren-at-uw.edu Name: Shirley Ren
Organization: University of Washington
Title-Subject: [Filtered] Covid-19 virus Message: Dear Colleagues, Has someone found the virus under TEM? Is it possible to do RNA-FISH on thick sections (Epoxy embedded block)? Many thanks,
Shirley
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X-from: Beck, Stephen J. {Stephen.Beck-at-ncc.edu}
Hi David,
Thanks for the reply. I’m not sure how I could get samples to you. My students were just working on getting samples prepared. My microbial/Paramecium prep was canceled by the corona virus. My students and I can’t even get on campus at present. Do you have any “stock” biological samples on hand? Can you even get to your own scopes? My students have required samples to image but I’m sure I’ll have to cut that back given the current situation. They would image 2 soft tissues, a cryofractured soft tissue, hard tissue, botanical sample (leaf stomates), pollen, microbial/Paramecium, insect, diatoms. Perhaps I could do a trial demo with you initially?
Thanks,
Steve
Stephen J. Beck Professor Coordinator, Bio-Imaging Center Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530
Sent from my iPhone - thanks Steve (1955-2011)!
} On Mar 22, 2020, at 12:46 PM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } } X-from: David Huskisson {david-at-emanalytical.co.uk} } } } Hi Stephen, } } We have a couple of our SEMs connected to the internet for remote access. Through this interface, } the microscope is completely controllable from your own PC. We can load your samples and then you or } your students would be able to take control of the instrument and practice from your own homes. } } Let me know how we can help. } } Kind regards, } } /David Huskisson, PhD./ } Project Scientist & Microscopist } } } Alderley Park } Macclesfield } SK10 4TG } } Office: +44 (0) 1625 704 467 } DD: +44 (0) 1625 238 869 } Mob: +44 (0) 7837 718 098 } } www.emanalytical.co.uk {http://www.emanalytical.co.uk/} } } } */Confidentiality Notice: /* } } This message is private and confidential. If received in error, please destroy and notify sender. } Sender does not intend to waive confidentiality or privilege. Dissemination, use of or reliance upon } this email is prohibited when received in error. Email may be susceptible to data corruption, } interception and unauthorised amendment, and no liability is accepted by the sender for any of the } foregoing. It is the recipient’s responsibility to scan the email and any attachment for viruses. } } } } On Sat, Mar 21, 2020 at 1:48 PM {microscopy.listserver-at-gmail.com } {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} , Microscopy-at-Microscopy.com so that all } Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck } } Organization: Nassau Community College } } Title-Subject: [Filtered] Teaching SEM Online } } Message: Dear Colleagues, } I am teaching my SEM course this semester and like many institutions, we are trying to teach } online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam } online, } however, after that we need to get on the SEM to image the required samples. } Does anyone have any ideas regarding teaching SEM online? 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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both drk-at-shcc.org, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: drk-at-shcc.org Name: Doug Keene
Organization: Shriners Hospital for Childern
Title-Subject: [Filtered] I'd like to help with Corona virus research
Message: Hello Everyone,
I'd like to apply my talents in Microscopy (TEM, SEM, Confocal, Micro-CT) to the Corona pandemic. How do I get the word out that I would like to contribute?
Thanks,
Doug Keene Shriner Hospital for Children Portland, Oregon 503-819-3600 (cell)
Login Host: 76.105.214.204 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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X-from: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
It seems that you replied to the entire list. Since you did, there should be many scopes in many labs that should be able to help. The FEI scopes are setup to use UltraVNC as part of their RAPID support service. It can be used just as well by lab staff and clients for their own purposes. (In fact, I have used it more for my own benefit than FEI has ever used it for theirs.) It would need to be setup properly with a single computer controlling the computer. Multiple others might be able to watch along. I would offer to help, but I am in the materials science area and would barely know what I am looking at. We also do not have samples handy for demo purposes. I have a counterpart that would have such expertise and samples, but she runs a Hitachi SEM and it is not setup for remote control or viewing. Warren Straszheim, Ph.D., manager Materials Analysis and Research Lab Iowa State University 515-294-8187
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, March 24, 2020 8:32 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
X-from: Beck, Stephen J. {Stephen.Beck-at-ncc.edu}
Hi David,
Thanks for the reply. I’m not sure how I could get samples to you. My students were just working on getting samples prepared. My microbial/Paramecium prep was canceled by the corona virus. My students and I can’t even get on campus at present. Do you have any “stock” biological samples on hand? Can you even get to your own scopes? My students have required samples to image but I’m sure I’ll have to cut that back given the current situation. They would image 2 soft tissues, a cryofractured soft tissue, hard tissue, botanical sample (leaf stomates), pollen, microbial/Paramecium, insect, diatoms. Perhaps I could do a trial demo with you initially?
Thanks,
Steve
Stephen J. Beck Professor Coordinator, Bio-Imaging Center Electron Microscopy Department of Biology Nassau Community College Garden City, NY 11530
Sent from my iPhone - thanks Steve (1955-2011)!
} On Mar 22, 2020, at 12:46 PM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } } } X-from: David Huskisson {david-at-emanalytical.co.uk} } } } Hi Stephen, } } We have a couple of our SEMs connected to the internet for remote } access. Through this interface, the microscope is completely } controllable from your own PC. We can load your samples and then you or your students would be able to take control of the instrument and practice from your own homes. } } Let me know how we can help. } } Kind regards, } } /David Huskisson, PhD./ } Project Scientist & Microscopist } } } Alderley Park } Macclesfield } SK10 4TG } } Office: +44 (0) 1625 704 467 } DD: +44 (0) 1625 238 869 } Mob: +44 (0) 7837 718 098 } } www.emanalytical.co.uk {http://www.emanalytical.co.uk/} } } } */Confidentiality Notice: /* } } This message is private and confidential. If received in error, please destroy and notify sender. } Sender does not intend to waive confidentiality or privilege. } Dissemination, use of or reliance upon this email is prohibited when } received in error. Email may be susceptible to data corruption, } interception and unauthorised amendment, and no liability is accepted by the sender for any of the foregoing. It is the recipient’s responsibility to scan the email and any attachment for viruses. } } } } On Sat, Mar 21, 2020 at 1:48 PM {microscopy.listserver-at-gmail.com } {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------- } ------ } } X-from: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} , Microscopy-at-Microscopy.com so that all } Microscopy Listserver Subscribers can } benefit from our collective wisdom } } ---------------------------------------------------------------------- } ----- } } Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: } Steve Beck } } Organization: Nassau Community College } } Title-Subject: [Filtered] Teaching SEM Online } } Message: Dear Colleagues, } I am teaching my SEM course this semester and like many institutions, we are trying to teach } online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam } online, } however, after that we need to get on the SEM to image the required samples. } Does anyone have any ideas regarding teaching SEM online? 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I have tried to visualize E cloi capsular polysaccharide by following the protocol from the book chapter "Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell" by Sven Hammerschmidt and Manfred Rohde from the published book entitled "Streptococcus pneumoniae, Method and protocol" published by Humana Press. I compared alcian blue and ruthenium red in the presence or absence of lysine.
At least in this first trial, ruthenium red in the presence of lysine appeared to be superb among all conditions tested in our study setup. However, based on the EM analysis, I sensed that the preservation of CPS is suboptimal and suggesting room for improvement.
I was wondering if CPS experts could give me any suggestions about how I can improve the presentation of CPS. Many thanks,
Dear Hiro, I would recommend checking the review Ruthenium Red and the Bacterial Glycocaly by Fassel et al. (1999) https://doi.org/10.3109/10520299909047974 it compares several different fixation and staining protocols with indications of what works best for which type of CPS. best regards! Rob
--- Dr. Rob Mesman Post-Doc Department of Microbiology Faculty of Science Radboud University Nijmegen Heyendaalseweg 135 NL-6525 AJ Nijmegen The Netherlands
On 2020-03-24 14:43, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: hiro uryu {hiro.uryu-at-emscopic.com} } } } Dear List, } } I have tried to visualize E cloi capsular polysaccharide by following } the protocol from the book } chapter "Electron Microscopy to Study the Fine Structure of the } Pneumococcal Cell" by Sven } Hammerschmidt and Manfred Rohde from the published book entitled } "Streptococcus pneumoniae, Method } and protocol" published by Humana Press. I compared alcian blue and } ruthenium red in the presence or } absence of lysine. } } At least in this first trial, ruthenium red in the presence of lysine } appeared to be superb among } all conditions tested in our study setup. However, based on the EM } analysis, I sensed that the } preservation of CPS is suboptimal and suggesting room for improvement. } } I was wondering if CPS experts could give me any suggestions about how } I can improve the } presentation of CPS. Many thanks, } } Sincerely, } Hiro } ------ } Kunihiro Uryu, } } } ==============================Original } Headers============================== } 8, 53 -- From microscopy.listserver-at-gmail.com Tue Mar 24 08:33:05 2020 } 8, 53 -- Received: from mail-il1-f178.google.com } (mail-il1-f178.google.com [209.85.166.178]) } 8, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id } 02ODX5La027708 } 8, 53 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2020 08:33:05 } -0500 } 8, 53 -- Received: by mail-il1-f178.google.com with SMTP id } r5so11998874ilq.6 } 8, 53 -- for {microscopy-at-microscopy.com} ; Tue, 24 Mar 2020 } 06:33:19 -0700 (PDT) } 8, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=gmail.com; s=20161025; } 8, 53 -- } h=subject:references:to:from:message-id:date:user-agent:mime-version } 8, 53 -- } :in-reply-to:content-language:content-transfer-encoding; } 8, 53 -- bh=RT4nGuTjtbspSuKPh6kiwaS5fInU0499H0wIMC8Wcy4=; } 8, 53 -- } b=a8i1Xg70t6DbybebaIpDmD67r6FLD7UgJmbNbIF17+AYFKSMAD6SrKU31oNbqlVlmk } 8, 53 -- } qqAa8aVYtMSWevsO1ixWsB0XSpQnJCnBbGyL273IpNwrQyJhvxlPEF4WkjnUO33TloAo } 8, 53 -- } vrWq3fAlyEhO2fvvSCQI0M9Gpx/uEjATzrphfd/xymYSANuLTRYZXptfMIdeZf50jPgr } 8, 53 -- } ycJ/wnluKgmeFUfVow8C9Hbaaieg7MxiKGggBecub2LyX6980QVFLN5Qjv8RQc3YGS4/ } 8, 53 -- } P6qRpG83Mdwvx/UV2pp01agxAvZa4Bw5ael3gU2AhwkpWVVXgVrE2pHQBAweKQxiEFO9 } 8, 53 -- hMKQ== } 8, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=1e100.net; s=20161025; } 8, 53 -- } h=x-gm-message-state:subject:references:to:from:message-id:date } 8, 53 -- :user-agent:mime-version:in-reply-to:content-language } 8, 53 -- :content-transfer-encoding; } 8, 53 -- bh=RT4nGuTjtbspSuKPh6kiwaS5fInU0499H0wIMC8Wcy4=; } 8, 53 -- } b=b/Bnly+nsTxA0HQN7J37XrrPa2ckB6Z3tS5T8AyYA2wxJlmhbH/XB7yR4DlVnT5D2P } 8, 53 -- } WukntUke2wlV11xyRKQvZntIhQByFAXozEw/dtE8wLc1x5KAhmyQYmo5Vv2c3Wo7X/G7 } 8, 53 -- } 3BEQjV46o0a8k4HxE++GNKTIx50PbF/UjG+BPws0gsQ/1Q7GbaEFcxCb3kd9oRNGoaoZ } 8, 53 -- } 8gL1Rsi96P/JOvm6aEGrCaWzTCw0Rw+XVek1ImE286gIedxBgXYeH4W058oSytIjH4mk } 8, 53 -- } xzGTRf17BdCQOwWUJvX1MR+WP+0kyVJbnaQoe6v5hSyG3O3pmt3Wlt0OL+QrFJCpXSdG } 8, 53 -- fqXQ== } 8, 53 -- X-Gm-Message-State: } ANhLgQ0lgx7paBjSX48pHPI2WRaYxiNPcUfDYFs5PcXMkInj1qloZ9+P } 8, 53 -- x2nc3YluGQUtapE+w3c3Vzv2L4vY } 8, 53 -- X-Google-Smtp-Source: } ADFU+vvPHf9jO3asg5Gs80mntLOFMBZ2ZgSV9OAtoVA8vI9svoUk9Yn+w2cZRJLnLD7//Jg2z/C/Hw== } 8, 53 -- X-Received: by 2002:a92:7eda:: with SMTP id } q87mr25309489ill.179.1585056798648; } 8, 53 -- Tue, 24 Mar 2020 06:33:18 -0700 (PDT) } 8, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:7174:d30f:fad6:337f]) } 8, 53 -- by smtp.googlemail.com with ESMTPSA id } s7sm5102890iob.53.2020.03.24.06.33.18 } 8, 53 -- for {microscopy-at-microscopy.com} } 8, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 } bits=128/128); } 8, 53 -- Tue, 24 Mar 2020 06:33:18 -0700 (PDT) } 8, 53 -- Subject: Fwd: E coli capsule polysaccharide } 8, 53 -- References: } {CADderMKZfYLMkSt0OfnMPDfcw0_o9Uq1OW_LdAFuLenSg61EXQ-at-mail.gmail.com} } 8, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 8, 53 -- X-Forwarded-Message-Id: } {CADderMKZfYLMkSt0OfnMPDfcw0_o9Uq1OW_LdAFuLenSg61EXQ-at-mail.gmail.com} } 8, 53 -- Message-ID: {af421fd8-b9b8-0147-b29d-6826422afe0c-at-gmail.com} } 8, 53 -- Date: Tue, 24 Mar 2020 08:33:17 -0500 } 8, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; } rv:68.0) } 8, 53 -- Gecko/20100101 Thunderbird/68.6.0 } 8, 53 -- MIME-Version: 1.0 } 8, 53 -- In-Reply-To: } {CADderMKZfYLMkSt0OfnMPDfcw0_o9Uq1OW_LdAFuLenSg61EXQ-at-mail.gmail.com} } 8, 53 -- Content-Type: text/plain; charset=utf-8; format=flowed } 8, 53 -- Content-Language: en-US } 8, 53 -- Content-Transfer-Encoding: 8bit } ==============================End of - } Headers==============================
Hi everyone, I have a full FEI remote package that we bought with our Themis Z but I wondered if there was another solution to remote TEM that people are using. I have a spare set of hand panels already.
Chris He/Him/His Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility
Dear Dr. Kunihiro Uryu, seconding Dr. Rob Mesman: fixation conditions…
Unfortunately not in possession of the protocol Dr. Kunihiro Uryu quoted*) only „active working“ colleagues might have the protocol in their collection, read and apply or have an answer for your request.
*) only for convenience: https://www.ncbi.nlm.nih.gov/pubmed/30929202 Methods Mol Biol. 2019;1968:13-33. doi: 10.1007/978-1-4939-9199-0_2.
Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell. Hammerschmidt S1, Rohde M2. Author information Abstract Electron microscopy allows for studying bacterial ultrastructure at high resolutions. Two types of electron microscopes are used for this purpose. The transmission electron microscope allows for access to inner bacterial ultrastructure when imaging ultrathin sections as well as cell wall-attached structures by negative staining, whereas scanning electron microscopy allows for the detection of structures on the bacterial cell surface alone or to study the interplay between pneumococci and their host cells. This chapter deals with recommendations for well-adapted methodologies to examine pneumococcal ultrastructure in detail. Especially, we focus on the preservation of the pneumococcal capsular polysaccharide, which represents an important virulence factor of pneumococci. Since capsules are highly hydrated structures, the introduction of a new fixation protocol involving lysine acetate, ruthenium red, and osmium (LRR fixation) results in a very well-preserved capsular structure in such a way that the amount of capsular material bound on the bacterial surface can be compared within different serotypes. In our method, capsular ultrastructure is preserved without the need for serotype-specific antibodies, which have been used in other studies to preserve the pneumococcal capsule. In addition, the new LRR fixation allows for studying the presence or absence of capsular material during adhesion and invasion of pneumococci on epithelial or endothelial host cells in cell culture experiments. KEYWORDS: Critical point drying; Cryo-FESEM; Field emission scanning electron microscopy; Infection; LRR embedding; LRWhite resin; Pneumococcal capsule; Pneumococci; Transmission electron microscopy PMID: 30929202 DOI: 10.1007/978-1-4939-9199-0_2 [Indexed for MEDLINE] ⇒SPRINGER: https://link.springer.com/protocol/10.1007%2F978-1-4939-9199-0_2
It would be interesting whether you tried "additives" like e.g. TA, La+++ and / or PPD (at least for a part of your specimens) with regard to fixation of the bacteria**) (ok, I know that E. coli is something different from....but you might have a look into my Poster https://www.researchgate.net/publication/215824406_Purpura_fulminans_ultrastructural_findings_by_transmission_electron_microscopy_in_four_cases_with_special_reference_to_fixation_of_skin_biopsies and the oral presentation, uploaded into RG https://www.researchgate.net/publication/281120633_Oral_Lecture_PURPURA_FULMINANS_Ultrastructural_findings_by_Transmission_Electron_Microscopy_in_4_cases_with_special_reference_to_fixation_of_skin_biopsies
Eventually you might have tried also the variation (".....substituting ruthenium red with a 0.5% alcian blue pyridine variant (pH 7.2).), cf. https://www.researchgate.net/publication/313875937_Molecular_characterization_of_invasive_capsule_null_Neisseria_meningitidis_in_South_Africa https://www.researchgate.net/figure/Transmission-electron-micrographs-showing-the-presence-of-surface-capsular-polysaccharide
Beware of Covid-19/SARS-CoV-2 infection, keep distance and STAY WELL,
best wishes AND best regards
Wolfgang Muß (MUSS) Retired Member of MSA SALZBURG, Austria
**) what I wanted to say with this is only pointing to other possibilities for retaining otherwise eluted "cellular or matricial substrate(s) in preparation for TEM.... (Sorry if I bothered anyone in this time of obviously global crisis)
====================================================================== Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Dienstag, 24. März 2020 15:10 An: wij.muss-at-aon.at Betreff: [Microscopy] Re: E coli capsule polysaccharide
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X-from: Rob Mesman {R.Mesman-at-science.ru.nl}
Dear Hiro, I would recommend checking the review Ruthenium Red and the Bacterial Glycocaly [Glycocalyx] by Fassel et al. (1999) https://doi.org/10.3109/10520299909047974 it compares several different fixation and staining protocols with indications of what works best for which type of CPS. best regards! Rob
--- Dr. Rob Mesman Post-Doc Department of Microbiology Faculty of Science Radboud University Nijmegen Heyendaalseweg 135 NL-6525 AJ Nijmegen The Netherlands
On 2020-03-24 14:43, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } X-from: hiro uryu {hiro.uryu-at-emscopic.com} } } } Dear List, } } I have tried to visualize E cloi capsular polysaccharide by following } the protocol from the book chapter "Electron Microscopy to Study the } Fine Structure of the Pneumococcal Cell" by Sven Hammerschmidt and } Manfred Rohde from the published book entitled "Streptococcus } pneumoniae, Method and protocol" published by Humana Press. I } compared alcian blue and ruthenium red in the presence or absence } of lysine. } } At least in this first trial, ruthenium red in the presence of lysine } appeared to be superb among all conditions tested in our } study setup. However, based on the EM analysis, I sensed that the } preservation of CPS is suboptimal and suggesting room for improvement. } } I was wondering if CPS experts could give me any suggestions about how } I can improve the presentation of CPS. 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==============================Original Headers============================== 23, 20 -- From wij.muss-at-aon.at Tue Mar 24 09:57:51 2020 23, 20 -- Received: from bsmtp2.bon.at (bsmtp2.bon.at [213.33.87.16]) 23, 20 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 02OEvoCG026169 23, 20 -- for {Microscopy-at-Microscopy.com} ; Tue, 24 Mar 2020 09:57:51 -0500 23, 20 -- Received: from MussTHINK (unknown [93.83.25.98]) 23, 20 -- by bsmtp2.bon.at (Postfix) with ESMTPSA id 48mvWJ0Vwcz5tl9 23, 20 -- for {Microscopy-at-Microscopy.com} ; Tue, 24 Mar 2020 15:58:03 +0100 (CET) 23, 20 -- From: "MUSS Wolfgang Dr. phil./PhD \(OR i.R, retired\)" {wij.muss-at-aon.at} 23, 20 -- To: {Microscopy-at-Microscopy.com} 23, 20 -- Subject: [Microscopy] Re: E coli capsule polysaccharide 23, 20 -- Date: Tue, 24 Mar 2020 15:58:04 +0100 23, 20 -- Message-ID: {007201d601ec$9f1fa850$dd5ef8f0$-at-aon.at} 23, 20 -- MIME-Version: 1.0 23, 20 -- Content-Type: text/plain; 23, 20 -- charset="UTF-8" 23, 20 -- X-Mailer: Microsoft Outlook 15.0 23, 20 -- Thread-Index: AdYB7EW7KJd4afC2S/qdEozFvxB1WQ== 23, 20 -- Content-Language: de 23, 20 -- Content-Transfer-Encoding: 8bit 23, 20 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 02OEvoCG026169 ==============================End of - Headers==============================
While there are many software solutions for sharing desktops, they all fail a similar hurdle - you have to have your microscope PC connected to the network directly. This is NOT a good idea as your Themis computer will not be protected from the internet.
Over the last few months I've been suing a different solution on our "Classic" Titan cubed. Making use of KVM over IP gear. These consist of sender boxes that have HDMI and USB (and audio) inputs that connect (and can be powered by) Cat5 cables to your network. Then any number of receiver boxes can be used with HDMI/USB outputs anywhere you have a network port. These systems have MANY advantages over the FEI remote software;
1) They are FAST - they do real time up to 4k - not just 10fps you will be lucky to get over VNC 2) There is no intrinsic connection between the microscope and the network - as such there is no security issue (except if someone managed to work out your IP/passwords for the boxes, or put a virus on a USB stick - but this is just be careful..) 3) You can have multiple receivers for every sender - so many people can watch each instrument. 4) USB can be used for the hand panels, keyboard and mice for the computers. 5) Partner these with HDMI switches behind monitors and you can switch between local/remote super quickly. 6) They are microscope/instrument agnostic - if the instrument uses USB and HDMI etc it will work.
Downsides;
1) They work with little configuration inside a subnet (such an individual building - our situation where we have been running the instrument from multiple different locations due to building work) but if you are outside the subnet you'll need some help from your network people. 2) The boxes cost - on the order of $500 for each box. But compared to a microscope.. 3) And you'll need one sender for each screen.
I've been using boxes made by Kramer (I have NO connection to this company) but there are many manufacturers (Lindy, geffen etc). They are mostly used is point of sale displays (all those screens at malls and movie theatres) so they are pretty ubiquitous. I have three boxes (left screen, right screen and accessory screen which runs Gatan, Bruker, EMPAD AND support computer through a remote controlled switch). This has worked really well. Let me know if you have any follow up questions.
Cheers
Matthew
-- Dr Matthew Weyland Joint Deputy Director - Monash Centre for Electron Microscopy Associate Professor - Department of Materials Science and Engineering
Monash Centre for Electron Microscopy 10 Innovation Walk, Clayton Campus Monash University Clayton VIC 3800 Australia
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Hi everyone, I have a full FEI remote package that we bought with our Themis Z but I wondered if there was another solution to remote TEM that people are using. I have a spare set of hand panels already.
Chris He/Him/His Christopher J. Gilpin Ph.D. Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility
After 12+ great years at the UBC BioImaging Facility, I am moving on to a new position in the private sector next month! As such, my position here is available now and has been posted to the UBC HR website:
This is a great opportunity for someone with experience in various advanced TEM techniques including electron tomography, cryo TEM, HRTEM, and their associated sample prep and image processing techniques. We have also just moved the facility into brand new lab space, so the world will be your oyster! There is opportunity to work with our SEM and optical microscope instruments as well.
Concurrently, we are also looking for an optical microscopy specialist to run our three laser scanning confocal instruments, including an Olympus Multiphoton system, a Perkin-Elmer spinning disk, and their associated equipment. https://www.hr.ubc.ca/careers-postings/staff-s.php Posting ID: 36794
I am happy to answer other questions about the TEM tech position, but please direct any formal inquiries to our facility manager, Miki Fujita. BIF.manager-at-ubc.ca Cheers, Bradford Ross Electron Microscopy Technician BioImaging Facility University of British Columbia Biological Sciences Rm. 0120 6270 University Blvd. Vancouver, B.C. V6T 1Z4 phone 604-822-6996 ==============================Original Headers============================== 7, 47 -- From bradford.ross-at-botany.ubc.ca Tue Feb 11 14:26:05 2020 7, 47 -- Received: from vmaprod2.mail-relay.ubc.ca (vmaprod2.mail-relay.ubc.ca [142.103.117.133]) 7, 47 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 01BKQ2wI000320 7, 47 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2020 14:26:02 -0600 7, 47 -- Received: from vmaprod2.mail-relay.ubc.ca (localhost.localdomain [127.0.0.1]) 7, 47 -- by localhost (Email Security Appliance) with SMTP id B03D162D115_E42FF10B 7, 47 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2020 19:22:56 +0000 (GMT) 7, 47 -- Received: from mx3.mail-relay.ubc.ca (lbpglfsc01gstp01-ents01-f5-vrfglue-float.systems.it.ubc.ca [10.45.24.97]) 7, 47 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 7, 47 -- (Client CN "mx3.mail-relay.ubc.ca", Issuer "mx3.mail-relay.ubc.ca" (not verified)) 7, 47 -- by vmaprod2.mail-relay.ubc.ca (Sophos Email Appliance) with ESMTPS id 5C0C962A5FB_E42FF10F 7, 47 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2020 19:22:56 +0000 (GMT) 7, 47 -- Received: from smtp.mail.ubc.ca (lbpglfsc01gstp01-ents01-f5-vrfglue-float.systems.it.ubc.ca [10.45.24.97]) 7, 47 -- (using TLSv1.2 with cipher AES256-GCM-SHA384 (256/256 bits)) 7, 47 -- (Client did not present a certificate) 7, 47 -- by mx3.mail-relay.ubc.ca (Postfix) with ESMTPS id BC8B415F648 7, 47 -- for {microscopy-at-microscopy.com} ; Tue, 11 Feb 2020 11:22:56 -0800 (PST) 7, 47 -- Received: from EXCH-SRV02AP.ead.ubc.ca (10.19.216.28) by 7, 47 -- EXCH-SRV01BP.ead.ubc.ca (10.19.216.29) with Microsoft SMTP Server 7, 47 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id 7, 47 -- 15.1.1847.3; Tue, 11 Feb 2020 11:22:51 -0800 7, 47 -- Received: from EXCH-SRV05AP.ead.ubc.ca (10.19.216.49) by 7, 47 -- EXCH-SRV02AP.ead.ubc.ca (10.19.216.28) with Microsoft SMTP Server 7, 47 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_GCM_SHA256) id 7, 47 -- 15.1.1847.3; Tue, 11 Feb 2020 11:18:55 -0800 7, 47 -- Received: from EXCH-SRV05AP.ead.ubc.ca ([fe80::c0d9:94b3:5718:3f80]) by 7, 47 -- EXCH-SRV05AP.ead.ubc.ca ([fe80::c0d9:94b3:5718:3f80%4]) with mapi id 7, 47 -- 15.01.1847.005; Tue, 11 Feb 2020 11:18:55 -0800 7, 47 -- From: "Ross, Bradford" {bradford.ross-at-botany.ubc.ca} 7, 47 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 47 -- Subject: Core Facility Research Tech Position Available 7, 47 -- Thread-Topic: Core Facility Research Tech Position Available 7, 47 -- Thread-Index: AQHV4Qy+GzcqnRAw0k+U9Bc56Rhg7A== 7, 47 -- Date: Tue, 11 Feb 2020 19:18:55 +0000 7, 47 -- Message-ID: {e676603a70694f9a847ea3ac343a7adc-at-botany.ubc.ca} 7, 47 -- Accept-Language: en-US, en-CA 7, 47 -- Content-Language: en-US 7, 47 -- X-MS-Has-Attach: 7, 47 -- X-MS-TNEF-Correlator: 7, 47 -- x-originating-ip: [137.82.85.238] 7, 47 -- x-pmwin-version: 4.0.4, Antivirus-Engine: 3.77.1, Antivirus-Data: 5.72 7, 47 -- Content-Type: text/plain; charset="iso-8859-1" 7, 47 -- MIME-Version: 1.0 7, 47 -- X-PMWin-Version: 4.0.4, Antivirus-Engine: 3.77.1, Antivirus-Data: 5.72 7, 47 -- X-SASI-RCODE: 200 7, 47 -- Content-Transfer-Encoding: 8bit 7, 47 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 01BKQ2wI000320 ==============================End of - Headers==============================
Does anybody happen to have an SAD-aperture assembly (aka field limiting aperture assembly, manual version) for a Jeol JEM2100F lying outside the microscope at the moment?
I would be really interested in a photo of it with some measurements, mainly the diameter and length of the rod.
Alternatively, a sketch with dimensions would also be great.
All the best,
Philip
PS: I'm trying to avoid opening up the microscope and getting drawings from Jeol is a lengthy procedure.
==============================Original Headers============================== 15, 49 -- From koeck-at-kth.se Tue Feb 18 09:24:18 2020 15, 49 -- Received: from smtp-3.sys.kth.se (smtp-3.sys.kth.se [130.237.48.192]) 15, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 01IFOHAs017009 15, 49 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2020 09:24:18 -0600 15, 49 -- Received: from smtp-3.sys.kth.se (localhost.localdomain [127.0.0.1]) 15, 49 -- by smtp-3.sys.kth.se (Postfix) with ESMTP id 25879A242 15, 49 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2020 15:21:34 +0100 (CET) 15, 49 -- X-Virus-Scanned: by amavisd-new at kth.se 15, 49 -- Received: from smtp-3.sys.kth.se ([127.0.0.1]) 15, 49 -- by smtp-3.sys.kth.se (smtp-3.sys.kth.se [127.0.0.1]) (amavisd-new, port 10024) 15, 49 -- with LMTP id VRJC0qWBXGaA for {microscopy-at-microscopy.com} ; 15, 49 -- Tue, 18 Feb 2020 15:21:33 +0100 (CET) 15, 49 -- Received: from exdb02.ug.kth.se (exdb02.ug.kth.se [192.168.32.112]) 15, 49 -- by smtp-3.sys.kth.se (Postfix) with ESMTPS id 3DAA3A281 15, 49 -- for {microscopy-at-microscopy.com} ; Tue, 18 Feb 2020 15:21:32 +0100 (CET) 15, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=kth.se; s=default; 15, 49 -- t=1582035693; bh=YFkqfWEL7AxKOjOPgs44CMGZs78Sm//g4ePcYboHFhU=; 15, 49 -- h=From:To:Subject:Date; 15, 49 -- b=PARXhxD1lKqXFGxroRSsrSkYZEJp+8zrNkFQLUmRAW48F9z85oFG6uRKz1FlY0Qa8 15, 49 -- +ANRgA9fYyVIeUixwBD8Tbvv5I2I5la5mr4+pG1IajwIwhFrmA4MAg/H1BiNj2GzB5 15, 49 -- fyNSqfnSHO5A9b8RiNj9YveW6OzNLb9khlg0sTBw= 15, 49 -- Received: from exdb03.ug.kth.se (192.168.32.113) by exdb02.ug.kth.se 15, 49 -- (192.168.32.112) with Microsoft SMTP Server (TLS) id 15.0.1473.3; Tue, 18 Feb 15, 49 -- 2020 15:21:08 +0100 15, 49 -- Received: from exdb01.ug.kth.se (192.168.32.111) by exdb03.ug.kth.se 15, 49 -- (192.168.32.113) with Microsoft SMTP Server (TLS) id 15.0.1473.3; Tue, 18 Feb 15, 49 -- 2020 15:21:08 +0100 15, 49 -- Received: from exdb01.ug.kth.se ([192.168.32.111]) by exdb01.ug.kth.se 15, 49 -- ([192.168.32.111]) with mapi id 15.00.1473.005; Tue, 18 Feb 2020 15:21:08 15, 49 -- +0100 15, 49 -- From: =?utf-8?B?UGhpbGlwIEvDtmNr?= {koeck-at-kth.se} 15, 49 -- To: "3dem-at-ncmri.ucsd.edu" {3dem-at-ncmri.ucsd.edu} , 15, 49 -- "microscopy-at-microscopy.com" 15, 49 -- {microscopy-at-microscopy.com} 15, 49 -- Subject: SAD-aperture assembly 15, 49 -- Thread-Topic: SAD-aperture assembly 15, 49 -- Thread-Index: AQHV5mahbPDcSWghfEuLD4b8dHeypg== 15, 49 -- Date: Tue, 18 Feb 2020 14:21:07 +0000 15, 49 -- Message-ID: {1582035668079.3393-at-kth.se} 15, 49 -- Accept-Language: sv-SE, en-US 15, 49 -- Content-Language: sv-SE 15, 49 -- X-MS-Has-Attach: 15, 49 -- X-MS-TNEF-Correlator: 15, 49 -- x-ms-exchange-transport-fromentityheader: Hosted 15, 49 -- x-originating-ip: [130.229.133.148] 15, 49 -- Content-Type: text/plain; charset="utf-8" 15, 49 -- MIME-Version: 1.0 15, 49 -- Content-Transfer-Encoding: 8bit 15, 49 -- X-MIME-Autoconverted: from base64 to 8bit by microscopy.com id 01IFOHAs017009 ==============================End of - Headers==============================
The Coln-Ramos lab at Yale University is looking for a research associate to provide ongoing electron microscopy work for a variety of projects in the lab. Under the direction of the Principal Investigator and working in collaboration with laboratory personnel, this position will be in charge of the design, collection, optimization and interpretation of electron microscopy research in the Coln-Ramos lab as it pertains to the ultrastructure of C. elegans nervous system. The position will prepare and process C. elegans worms for electron microscopy to better understand the function and structure of the C. elegans nervous system. More information about the position can be found here: https://www.nature.com/naturecareers/job/electron-microscopy-research-associate-yale-university-715443
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both tprozoro-at-ameslab.gov, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Title-Subject: [Filtered] Postdoctoral position at the Ames Laboratory
Message: The Division of Materials Science and Engineering (DMSE) at the Ames Laboratory, a Department of Energy National Laboratory affiliated with Iowa State University, is searching for a qualified Postdoctoral Research Associate.
We are looking for a motivated postdoctoral research associate to work on two projects focused on characterization of structure and liquid phase dynamics of beam-sensitive materials by using electron microscopy in-situ. The first project involves spatio-chemical characterization of polymer-nanoparticle complexes and DNA origami nanostructures in liquid phase. The second project involves monitoring cation mobility in low-contrast layered minerals in liquid phase and in-situ. We are looking for applicants with hands-on experience using aberration-corrected scanning transmission electron microscopy (S/TEM), electron energy loss spectroscopy (EELS), and energy dispersive spectroscopy (EDS). More details and application instructions can be found here: https://isu.wd1.myworkdayjobs.com/en-US/IowaStateJobs/job/Ames-IA/Postdoctoral-Research-Associate---Ames-Laboratory_R1870
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Dear All, I apologize for a job posting that appears containing an invalid link. Thanks for those of you who brought it to my attention. Here is the link to the Yale HR website, and it works. https://sjobs.brassring.com/TGnewUI/Search/Home/Home?partnerid=25053&siteid=5248#jobDetails=1405083_5248.
Thanks you for your attention.
Xinran Nick Liu, M.D. & Ph.D. Director, Center for Cellular and Molecular Imaging Bio & Cryo Electron Microscopy Core Facilities Yale University School of Medicine Office: (203) 785-4050 Lab: (203) 785-5390 http://medicine.yale.edu/ccmi/em
Dear Collogues, The Colón-Ramos lab at Yale University is looking for a research associate to provide ongoing electron microscopy work for a variety of projects in the lab. Under the direction of the Principal Investigator and working in collaboration with laboratory personnel, this position will be in charge of the design, collection, optimization and interpretation of electron microscopy research in the Colón-Ramos lab as it pertains to the ultrastructure of C. elegans nervous system. The position will prepare and process C. elegans worms for electron microscopy to better understand the function and structure of the C. elegans nervous system. More information about the position can be found here: https://www.nature.com/naturecareers/job/electron-microscopy-research-associate-yale-university-715443 Thanks for your attention.
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both dkc25-at-cam.ac.uk, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: dkc25-at-cam.ac.uk Name: Darran Clements
Organization: University of Cambridge
Title-Subject: [Filtered] Stitching confocal volumes of organ in 3 dimensions
Message: Hi All, just wanted to throw out a general question to the list. We are currently considering embarking on a project where we have been asked to reconstruct an organ from mosaics of confocal volumes in serial sections. We can stitch volumes in each section together easily enough, however, I wanted to ask if anyone else has experience of then stitching these kinds of volumes together to reconstruct the whole organ volume, and if so, could they impart some advice on what they found works best.
We are looking at slices of tissue around 100 microns thick and around 80-100 of these volumes stitched together, then stitching multiple of these volume mosaics, one above the other, to reconstruct the tissue.
We're mainly looking at the software aspect of this but of course, tangential replies and alternative ideas are more than welcome too.
Many thanks Darran
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By the way, the solid angle would be the active area of the detector divided by the square of the distance to specimen.
Krassimir.
} On Feb 19, 2020, at 2:45 PM, nizets2-at-yahoo.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear colleagues, } } I'd have a simple question regarding the detector area of EDX (EDS) systems in SEM: } Clearly the largest the detector, the highest the sensitivity for everything else equal but what if everything else was NOT equal? } } One clear example: } } - detector 1: 30mm², optimal distance to pole piece 10mm } - detector 2: 150mm², optimal distance to pole piece 15mm } } Here the largest detector is also the furthest! } } I don't know how to appreciate the influence of the 2 factors, detector area and work distance, on the final sensitivity of the system. } In other words, is the extra $$$ for the larger detector worth it if the work distance is at the same time increased? } } Many thanks in advance. } Stephane } } } ==============================Original Headers============================== } 8, 36 -- From nizets2-at-yahoo.com Wed Feb 19 16:40:48 2020 } 8, 36 -- Received: from sonic306-48.consmr.mail.gq1.yahoo.com (sonic306-48.consmr.mail.gq1.yahoo.com [98.137.68.111]) } 8, 36 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 01JMelux001954 } 8, 36 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2020 16:40:48 -0600 } 8, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1582148289; bh=IDWrBC77DmQ8crCMi3VeBrepgESkQS9w38Lnx1B7gCA=; h=Date:From:To:Subject:References:From:Subject; b=Z5qqm6sz+L42XrA1L8rOQdfY6SYUoPrW1kaa7PuNRtfJpQgkW/usMK/A5gNKEzH/L/AwjzemL9yhlSoEZv6GJxXtgjHmqRu9bPrd2u4NHmp+VRGpoEZb+zV2kwx2sPwJKOR9NRtnRpc/1+wSzS7vGk8ya71C0gMU7f4pf2vwSqNtOME0KSJbR4SjjcFnp8PaxFUmsjP0ijnoE76mBjiWHqiyBJn1j6cBf4bJXJPQlQiZ4F1OlliLFOYk81jM0pQPgwgqF/om/P8+9OKz9a1xIeLBnKkwnKt2dBjhZeZ1tFpruTLdR1LWZjIPOmWKZfV82Qmgcek1O7KtFH4F/x9e8w== } 8, 36 -- X-YMail-OSG: KcDAD2IVM1miC7U6QuOqrAbAE3ZpEChGohU34TtHPElCud1VQEfiO8GR6tBqwW0 } 8, 36 -- WqHRIUFFUqKg6sXLtvXKj7d0PS7vHc41I8dv1SCKucrTUKw7RpEJLkcussukkz9yd.b0.uvFE9xs } 8, 36 -- FfqtjLBqgAV0mh4QmDfJI.quhjfONSWBwUL30JlVTe.EqrE.llX75rjZ.Je8vC7uJWbwlXo3VzjG } 8, 36 -- pbzdcFWDSous2wcm9RfCGRJBEL9HGHDSTBNQ2bOcOXsGAr_mpacd0kmX9CpOiP910On_xWwh_37y } 8, 36 -- lkOPBXDHES_X.MLBsNz8m1JxPj4vRM_dG1JgiOCbVyePA2V78i2htZvBdJ4Yfoxfn5EBg_4TdfZ4 } 8, 36 -- sgUy8IbcjKGCGKjjzSKihRM6D.QMwbZB9GruGnIa4rUWfU6Wv18eKYfBYHvhufSyCcnp4WESYX6B } 8, 36 -- tstgB93l.t8f2E9XV2XZHkEF980bP3X6oS1h5zZ_0EqPPdynJPFdMRCz7yPyJl4f3zgcNB3Zd1XM } 8, 36 -- 08yETtbZoTjTA4eGqP8RLf2R5.FKfsc02UPriDFjnw3GURjL7zbDdvSUe5RWp8PXhTQ_0ztmZS0Q } 8, 36 -- taC2HKOh2369e6kypne13SBNyG45YVie63HGXsuUl6bzgltnS7zfvnFIRxTDMJlksEt.WhMXPgxU } 8, 36 -- EUE7d9XL9UBj1ed.JkvAvHGsZF8CS8kkJz5.JBFxXglEYAe.7kWpvhEc0jkOYRzpJmePAXNS.ivY } 8, 36 -- t_bLjEMwTKgnDLL9Z0F9f_t0IvpA7iYCaw2RZdARCcBLK1p3gtipfMinYe1O2xI7R1NRSJ4g9C_L } 8, 36 -- wBpY6G4I4bAzKaDvourEDIO.7U8_Htci6d1bPkl7IUB6btxD6coaH8yg5csJyjSGtzwJf7Z4jtUt } 8, 36 -- 7d_m.e5hGkqaNHTxM8531QqFJ68A6yH7isf69J3I37mlUp.di8feQpIZtGn0sFpZ.N9dY882OkA1 } 8, 36 -- LA0nDRw4aivOyeN9XTIeMQqtRv_kBmInKN8JV2r2axc_6qQC62IdNmFGYh_E4J5Lm4IPxm_7Zaa6 } 8, 36 -- a6eKuKdyvm1vEvF0VClCeTbqLuGVyX06wLxvv4g7rivJpxabKL2.ajnTDx_Cbf90inh7_LmUMQ4M } 8, 36 -- xlNjyk7kLSUwn_g0vrhNaliH_hUzDaoNgHdX8_ESQKmnOBzHA4KC250WyeRW3n4oSZSYkzKC0Isg } 8, 36 -- 7agAb9UJc_VkXWiqhKYVFOfAvaIq3he7w7iXAvn2bqgQkgPSsyPo29MEgXs9q88zmDKKAZf.GLxp } 8, 36 -- FKLHhT8dKDw2131DHpN4TwheOxUpohl9VWngZ6XU480qtLJrRt8IYkj9t7jiGauPcf.BABJPTm2w } 8, 36 -- 2fsHZFAYz9cs.QwKT6xG9viLb6vEcZA-- } 8, 36 -- Received: from sonic.gate.mail.ne1.yahoo.com by sonic306.consmr.mail.gq1.yahoo.com with HTTP; Wed, 19 Feb 2020 21:38:09 +0000 } 8, 36 -- Date: Wed, 19 Feb 2020 21:36:07 +0000 (UTC) } 8, 36 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 8, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 8, 36 -- Message-ID: {317764846.5589118.1582148167228-at-mail.yahoo.com} } 8, 36 -- Subject: question about EDS in SEM } 8, 36 -- MIME-Version: 1.0 } 8, 36 -- Content-Type: text/plain; charset=UTF-8 } 8, 36 -- References: {317764846.5589118.1582148167228.ref-at-mail.yahoo.com} } 8, 36 -- X-Mailer: WebService/1.1.15199 YMailNorrin Mozilla/5.0 (Windows NT 10.0; Win64; x64; rv:72.0) Gecko/20100101 Firefox/72.0 } 8, 36 -- Content-Transfer-Encoding: 8bit } 8, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 01JMelux001954 } ==============================End of - Headers==============================
We are looking after a traceable standard specimen for TEM calibration. We had a MAG*I*CAL sample that got damaged, and it is in a temporary backorder at the EMSDiasum website.
Does any of you know and have used another traceable standard specimen for TEM calibration?
Thank you,
-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
Go to this WWW site. It is an on-line solid angle calculator for XEDS systems.
http://www.zaluzec.com/NJZTools/
It will give you an unbiased calculation of solid angles.
Importantly I must contradict Krassimir, Area/distance^2 is incorrect, that is an approximation which quickly breaks down for large detectors and small distances.
On that Solid Angle WWW site is also a link to a paper you might want to read.
Cheers
Nestor Your Friendly Neighborhood SysOp
On 2/20/20 9:40 AM, nizets2-at-yahoo.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear colleagues, } } I'd have a simple question regarding the detector area of EDX (EDS) systems in SEM: } Clearly the largest the detector, the highest the sensitivity for everything else equal but what if everything else was NOT equal? } } One clear example: } } - detector 1: 30mm², optimal distance to pole piece 10mm } - detector 2: 150mm², optimal distance to pole piece 15mm } } Here the largest detector is also the furthest! } } I don't know how to appreciate the influence of the 2 factors, detector area and work distance, on the final sensitivity of the system. } In other words, is the extra $$$ for the larger detector worth it if the work distance is at the same time increased? } } Many thanks in advance. } Stephane } } } ==============================Original Headers============================== } 8, 36 -- From nizets2-at-yahoo.com Wed Feb 19 16:40:48 2020 } 8, 36 -- Received: from sonic306-48.consmr.mail.gq1.yahoo.com (sonic306-48.consmr.mail.gq1.yahoo.com [98.137.68.111]) } 8, 36 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 01JMelux001954 } 8, 36 -- for {microscopy-at-microscopy.com} ; Wed, 19 Feb 2020 16:40:48 -0600 } 8, 36 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=yahoo.com; s=s2048; t=1582148289; bh=IDWrBC77DmQ8crCMi3VeBrepgESkQS9w38Lnx1B7gCA=; h=Date:From:To:Subject:References:From:Subject; b=Z5qqm6sz+L42XrA1L8rOQdfY6SYUoPrW1kaa7PuNRtfJpQgkW/usMK/A5gNKEzH/L/AwjzemL9yhlSoEZv6GJxXtgjHmqRu9bPrd2u4NHmp+VRGpoEZb+zV2kwx2sPwJKOR9NRtnRpc/1+wSzS7vGk8ya71C0gMU7f4pf2vwSqNtOME0KSJbR4SjjcFnp8PaxFUmsjP0ijnoE76mBjiWHqiyBJn1j6cBf4bJXJPQlQiZ4F1OlliLFOYk81jM0pQPgwgqF/om/P8+9OKz9a1xIeLBnKkwnKt2dBjhZeZ1tFpruTLdR1LWZjIPOmWKZfV82Qmgcek1O7KtFH4F/x9e8w== } 8, 36 -- X-YMail-OSG: KcDAD2IVM1miC7U6QuOqrAbAE3ZpEChGohU34TtHPElCud1VQEfiO8GR6tBqwW0 } 8, 36 -- WqHRIUFFUqKg6sXLtvXKj7d0PS7vHc41I8dv1SCKucrTUKw7RpEJLkcussukkz9yd.b0.uvFE9xs } 8, 36 -- FfqtjLBqgAV0mh4QmDfJI.quhjfONSWBwUL30JlVTe.EqrE.llX75rjZ.Je8vC7uJWbwlXo3VzjG } 8, 36 -- pbzdcFWDSous2wcm9RfCGRJBEL9HGHDSTBNQ2bOcOXsGAr_mpacd0kmX9CpOiP910On_xWwh_37y } 8, 36 -- lkOPBXDHES_X.MLBsNz8m1JxPj4vRM_dG1JgiOCbVyePA2V78i2htZvBdJ4Yfoxfn5EBg_4TdfZ4 } 8, 36 -- sgUy8IbcjKGCGKjjzSKihRM6D.QMwbZB9GruGnIa4rUWfU6Wv18eKYfBYHvhufSyCcnp4WESYX6B } 8, 36 -- tstgB93l.t8f2E9XV2XZHkEF980bP3X6oS1h5zZ_0EqPPdynJPFdMRCz7yPyJl4f3zgcNB3Zd1XM } 8, 36 -- 08yETtbZoTjTA4eGqP8RLf2R5.FKfsc02UPriDFjnw3GURjL7zbDdvSUe5RWp8PXhTQ_0ztmZS0Q } 8, 36 -- taC2HKOh2369e6kypne13SBNyG45YVie63HGXsuUl6bzgltnS7zfvnFIRxTDMJlksEt.WhMXPgxU } 8, 36 -- EUE7d9XL9UBj1ed.JkvAvHGsZF8CS8kkJz5.JBFxXglEYAe.7kWpvhEc0jkOYRzpJmePAXNS.ivY } 8, 36 -- t_bLjEMwTKgnDLL9Z0F9f_t0IvpA7iYCaw2RZdARCcBLK1p3gtipfMinYe1O2xI7R1NRSJ4g9C_L } 8, 36 -- wBpY6G4I4bAzKaDvourEDIO.7U8_Htci6d1bPkl7IUB6btxD6coaH8yg5csJyjSGtzwJf7Z4jtUt } 8, 36 -- 7d_m.e5hGkqaNHTxM8531QqFJ68A6yH7isf69J3I37mlUp.di8feQpIZtGn0sFpZ.N9dY882OkA1 } 8, 36 -- LA0nDRw4aivOyeN9XTIeMQqtRv_kBmInKN8JV2r2axc_6qQC62IdNmFGYh_E4J5Lm4IPxm_7Zaa6 } 8, 36 -- a6eKuKdyvm1vEvF0VClCeTbqLuGVyX06wLxvv4g7rivJpxabKL2.ajnTDx_Cbf90inh7_LmUMQ4M } 8, 36 -- xlNjyk7kLSUwn_g0vrhNaliH_hUzDaoNgHdX8_ESQKmnOBzHA4KC250WyeRW3n4oSZSYkzKC0Isg } 8, 36 -- 7agAb9UJc_VkXWiqhKYVFOfAvaIq3he7w7iXAvn2bqgQkgPSsyPo29MEgXs9q88zmDKKAZf.GLxp } 8, 36 -- FKLHhT8dKDw2131DHpN4TwheOxUpohl9VWngZ6XU480qtLJrRt8IYkj9t7jiGauPcf.BABJPTm2w } 8, 36 -- 2fsHZFAYz9cs.QwKT6xG9viLb6vEcZA-- } 8, 36 -- Received: from sonic.gate.mail.ne1.yahoo.com by sonic306.consmr.mail.gq1.yahoo.com with HTTP; Wed, 19 Feb 2020 21:38:09 +0000 } 8, 36 -- Date: Wed, 19 Feb 2020 21:36:07 +0000 (UTC) } 8, 36 -- From: Stephane Nizet {nizets2-at-yahoo.com} } 8, 36 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} } 8, 36 -- Message-ID: {317764846.5589118.1582148167228-at-mail.yahoo.com} } 8, 36 -- Subject: [Filtered] question about EDS in SEM } 8, 36 -- MIME-Version: 1.0 } 8, 36 -- Content-Type: text/plain; charset=UTF-8 } 8, 36 -- References: {317764846.5589118.1582148167228.ref-at-mail.yahoo.com} } 8, 36 -- X-Mailer: WebService/1.1.15199 YMailNorrin Mozilla/5.0 (Windows NT 10.0; Win64; x64; rv:72.0) Gecko/20100101 Firefox/72.0 } 8, 36 -- Content-Transfer-Encoding: 8bit } 8, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 01JMelux001954 } ==============================End of - Headers============================== } }
Let me be sure I understand your question correctly. Your first option of a 30-mm2 detector would allow a 10-mm working distance. Your second option of a 150mm2 detector would require a 15-mm working distance to the pole piece. That does not say anything about the sample to detector distance which is required to calculate the solid angle. The 15-mm working distance might be your better option. That is similar to the situation we ran into. I wished I had posed the question to the list 9 years ago. - We had been using a 10mm2 detector on a Hitachi 2460N which was setup for a 25-mm working distance for the eucentric height and analytical distance. We were able to bring the EDS detector in below the pole piece so that it was quite close to the sample and gave us a pretty good solid angle. It did mean that the EDS detector was the first thing in harm's way. A user scratched up one of our aluminum sample platens when they raised it into the end of the detector then proceeded to move the sample around. - We upgraded to a FEI Quanta with field emission. It's eucentric height was 10-mm which was better for imaging. We knew we wanted to get a large detector in order to maintain as much image resolution as possible when doing EDS so we opted for an 80-mm2 detector figuring we would get 8x more counts at the same beam current. It turned out that we only got about 3x more. We had ordered the system configured to work at 10-mm working distance. With the design of the pole piece, that meant our detector had to stand about 70% further off than before. So we gained 8x from the detector area but lost almost 3x due to the greater distance. I have wondered about redoing our system to operate at 15-mm working distance and bring the detector in much closer. It would greatly improve our solid angle. If I was only doing EDS, I would probably go for it. It would mean different operating conditions for EDS versus just imaging. That might confuse some of our many users. We also have WDS installed, and I understand it is aimed at 10-mm. So, I will probably just leave things as they are and bring up the current a bit more. Now to the other question of performance and resolution as a function of size. It is true that all other things being equal, you may give up a little energy resolution by going to a bigger detector. It is harder to find a crystal of the same, good resolution when it has to be 8x larger, but it can be done. They will probably charge a premium for it. You probably want to compare spectra on your real samples. I choose to back off on the process time to push more counts at the same deadtime. So, I am already sacrificing some resolution. I am glad that I started with a better detector. There are times that I want for better resolution so I increase the process time and cut the count rate - or I switch over to the WDS. Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: nizets2-at-yahoo.com {nizets2-at-yahoo.com} Sent: Wednesday, February 19, 2020 4:41 PM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
Dear colleagues,
I'd have a simple question regarding the detector area of EDX (EDS) systems in SEM: Clearly the largest the detector, the highest the sensitivity for everything else equal but what if everything else was NOT equal?
One clear example:
- detector 1: 30mm², optimal distance to pole piece 10mm - detector 2: 150mm², optimal distance to pole piece 15mm
Here the largest detector is also the furthest!
I don't know how to appreciate the influence of the 2 factors, detector area and work distance, on the final sensitivity of the system. In other words, is the extra $$$ for the larger detector worth it if the work distance is at the same time increased?
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Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM sample preparation
Message: Hello,
I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use TEM to check the morphology/size distribution of the sample i.e. individual particles or agglomerates. I have prepared the samples by dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.
I am aware that due to drying of the solvent from the TEM grid, the carbon dots that are present in the drop are forced together and may form agglomerates. I would like to eliminate/minimize the agglomerate formation in the sample preparation process as it is influencing my analysis which is used to evaluate the synthesis method (formation of single particles/formation of agglomerates).
What are the possible methods I could use in my sample prep, to get the least possible particle agglomeration?
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We searched and after several years the answer was clearly no. We settled our traceable TEM calibration problem by calibrating with a replica grating and then averaging the measurement of 200 particles of a NIST traceable nanospheres. We selected the 200nm (actually203nm) traceable spheres and typically get numbers within 5%. There are smaller traceable spheres, but the 200 have the smallest Std deviation and expanded uncertainty,
Please let me know if you find a better solution.
Stay safe..
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
Orignal Message:
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X-from: Erico Freitas {ericotadeu-at-ufmg.br}
Dear all,
We are looking after a traceable standard specimen for TEM calibration. We had a MAG*I*CAL sample that got damaged, and it is in a temporary backorder at the EMSDiasum website.
Does any of you know and have used another traceable standard specimen for TEM calibration?
Thank you,
-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antnio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
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Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM sample preparation
Message: Hello,
I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use TEM to check the morphology/size distribution of the sample i.e. individual particles or agglomerates. I have prepared the samples by dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.
I am aware that due to drying of the solvent from the TEM grid, the carbon dots that are present in the drop are forced together and may form agglomerates. I would like to eliminate/minimize the agglomerate formation in the sample preparation process as it is influencing my analysis which is used to evaluate the synthesis method (formation of single particles/formation of agglomerates).
What are the possible methods I could use in my sample prep, to get the least possible particle agglomeration?
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Arunas, I forgot to mention UA is incompatible with phosphate and will precipitate in its presence. If you use it then briefly rinse in DH2O before the negative staining (after sample adsorbtion). Good Luck, Michael Delannoy Johns Hopkins SOM Microscope Facility
Dear Arunas, You probably already considered this, but whatever you see on TEM is not going to be representative of what's happening in solution, irrespective of preparation method. (Except, maybe freezing/Cryo_TEM?). If what you want to know is how they are dispersed in solution, then DLS or a similar light scattering technique might be best. 20nm particles are well within the size range that's possible. Although, I suppose there will be relatively little contrast due to the material, it should be feasible. In any case, it's a very quick method to try. Good luck, Pete. ____________________________________________________________________________ Atomic Force Microscopy Dr Peter Eaton and Dr Paul West Available through all good bookshops, or direct from Oxford University Press at: http://ukcatalogue.oup.com/product/9780199570454.do http://afmhelp.com
________________________________________ X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: 21 February 2020 22:25 To: petereaton-at-hotmail.com
To: Zaluzec-at-microscopy.com
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Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM sample preparation
Message: Hello,
I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use TEM to check the morphology/size distribution of the sample i.e. individual particles or agglomerates. I have prepared the samples by dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension.
I am aware that due to drying of the solvent from the TEM grid, the carbon dots that are present in the drop are forced together and may form agglomerates. I would like to eliminate/minimize the agglomerate formation in the sample preparation process as it is influencing my analysis which is used to evaluate the synthesis method (formation of single particles/formation of agglomerates).
What are the possible methods I could use in my sample prep, to get the least possible particle agglomeration?
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Look up "Ammonium Laurate Surfactant for Cleaner Deposition of Carbon Nanotubes" by Hannah Nisson, DOI:10.1021/acs.langmuir.5b01175 on using surfactants to avoid agglomeration during TEM sample prep.
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 2/26/2020 6:21 AM, petereaton-at-hotmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } Dear Arunas, } You probably already considered this, but whatever you see on TEM is not going to be representative of what's happening in solution, irrespective of preparation method. (Except, maybe freezing/Cryo_TEM?). If what you want to know is how they are dispersed in solution, then DLS or a similar light scattering technique might be best. 20nm particles are well within the size range that's possible. Although, I suppose there will be relatively little contrast due to the material, it should be feasible. In any case, it's a very quick method to try. } Good luck, } Pete. } ____________________________________________________________________________ } Atomic Force Microscopy } Dr Peter Eaton and Dr Paul West } Available through all good bookshops, or direct from Oxford University Press at: } http://ukcatalogue.oup.com/product/9780199570454.do http://afmhelp.com } } } ________________________________________ } X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} } Sent: 21 February 2020 22:25 } To: petereaton-at-hotmail.com } Subject: [Microscopy] viaWWW:Avoiding nanoparticle agglomeration in TEM sample preparation } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } To: Zaluzec-at-microscopy.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both arunas.mesceriakovas-at-uef.fi, } Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: arunas.mesceriakovas-at-uef.fi Name: Arûnas Meðèeriakovas } } Organization: University of Eastern Finland } } Title-Subject: [Filtered] Avoiding nanoparticle agglomeration in TEM } sample preparation } } Message: Hello, } } } I synthesize 20 nm sized individual amorphous carbon spheres/dots. I use } TEM to check the morphology/size distribution of the sample i.e. } individual particles or agglomerates. I have prepared the samples by } dipping the TEM grid in a tip sonicated water/Ethanol/carbon dot suspension. } } I am aware that due to drying of the solvent from the TEM grid, the } carbon dots that are present in the drop are forced together and may } form agglomerates. } I would like to eliminate/minimize the agglomerate formation in the } sample preparation process as it is influencing my analysis which is } used to evaluate the synthesis method (formation of single } particles/formation of agglomerates). } } What are the possible methods I could use in my sample prep, to get the } least possible particle agglomeration? } } Login Host: 193.167.228.180 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } } ==============================Original Headers============================== } 13, 53 -- From microscopy.listserver-at-gmail.com Fri Feb 21 16:24:33 2020 } 13, 53 -- Received: from mail-pf1-f180.google.com (mail-pf1-f180.google.com [209.85.210.180]) } 13, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 01LMOXxh006217 } 13, 53 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2020 16:24:33 -0600 } 13, 53 -- Received: by mail-pf1-f180.google.com with SMTP id s1so1901822pfh.10 } 13, 53 -- for {microscopy-at-microscopy.com} ; Fri, 21 Feb 2020 13:22:01 -0800 (PST) } 13, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 13, 53 -- d=gmail.com; s=20161025; } 13, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 13, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 13, 53 -- bh=AgZ7Q+ThYg4tKsmX2OzO9C21VnfQI3XQrXOjquhXKf4=; } 13, 53 -- b=tfDlZvElkm1nR5T/H3gSOFp+QE4BcIJqpNhyz6EuUROV7Hd923chs2aBLRLiRGkf3z } 13, 53 -- 4dWWb+gEidcOIGibW0h7mn7SPOGFlv0IRN+SSynCMwYnKdvEy2K0mEpvobIgdR4mLC2E } 13, 53 -- Hg16QjO5o8JCEhxp/ku8doudfv7bBcX1jPW8KYG6xZr7gxIuju/y6M4MIOADq1vcPDDo } 13, 53 -- 292LRPqe3hJUtUuIUMaddy1+5U3b6LtX+CGA/a4xprCZKtJWRiA8MhvsRwuX1wW6atZj } 13, 53 -- JaHHZzJz4YjifwSqzv3VaucCZZz9GaraADYB9J00Ip+3x3Bb7mkAvRzwOxAdi7QFIxfO } 13, 53 -- 5fJw== } 13, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 13, 53 -- d=1e100.net; s=20161025; } 13, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 13, 53 -- :user-agent:mime-version:in-reply-to:content-language } 13, 53 -- :content-transfer-encoding; } 13, 53 -- bh=AgZ7Q+ThYg4tKsmX2OzO9C21VnfQI3XQrXOjquhXKf4=; } 13, 53 -- b=GWWoctZ1bNfLY1sDyUEt2rWcVG4WW6B2HND40eHRLns4A6mLuiOZ3QdBUQFyUMmgva } 13, 53 -- q0yL05igk6Ql6oJlfpPwFYLYTr+aHZ2gZUCs5Ys9V+OloSKg9sbfHcLgMF1xezfVGqh3 } 13, 53 -- Ni/F3t+ynoIRSdCD+a2gP/jhMiPm0Lc7xTOKXd4ZTxEmQhrfA8fMnuiWWDkxF0ZWGsy1 } 13, 53 -- pdxXXCzGWpEWgbz+VimO62zMKX1835MZ8/xfynprOVMpFGg1ITe607Ii+LqZfySH0cWR } 13, 53 -- ihl+I/Uw53VK9FVQkPRmMsa9lzY3/FCmVUtHTZqN3YJLlSUQfwRoNJg6OLJkfIO8FzkU } 13, 53 -- csLA== } 13, 53 -- X-Gm-Message-State: APjAAAVqzkTe8GFWvX0sVLi0a6oj0w6NvQcd1DEY5seUy+11wAmmElCF } 13, 53 -- CJ4yUOzIroWyi144dCd4uE4H0RUZ } 13, 53 -- X-Google-Smtp-Source: APXvYqzLwQobnTnTS8ReSVCwYYHOccwU6ygcXmaQgYUXoZQRRjLVc9FFlSw9E3LyWGkw+2thRMQxKg== } 13, 53 -- X-Received: by 2002:a62:8e0a:: with SMTP id k10mr40655825pfe.49.1582320120226; } 13, 53 -- Fri, 21 Feb 2020 13:22:00 -0800 (PST) } 13, 53 -- Received: from Nestor-MacBookAir-Pro-2014-ElCapitan.local (ppp59-167-172-94.static.internode.on.net. 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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both hancocksk-at-vt.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: hancocksk-at-vt.edu
Name: Sandy Hancock
Organization: College of Veterinary Medicine, Virginia Tech
Title-Subject: [Filtered] EM Lab Manager position at CVM Virginia Tech
Message: Hello EM Colleagues,
The Electron Microscopy Laboratory at the College of Veterinary Medicine at Virginia Tech has a position open for a Lab Manager. The position is responsible for the day-to-day operations of the service laboratory, which primarily supports researchers on campus who submit biological samples, with the occasional nanoparticle/materials sample. The laboratory houses a JEOL JEM-1400 TEM, Zeiss EVO 40 SEM and supporting equipment for specimen preparation. More information can be found at the position listing http://careers.pageuppeople.com/968/cw/en-us/job/512843/electron-microscopy-lab-mgr
Thank you! Sandy
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Email: ralvaradojr-at-ufl.edu Name: Rudy Organization: University of Florida
Title-Subject: [Filtered] Buffers other than PBS to use for IEM
Message: Hi everyone, I am trying to process a strain of Lactobacillus johnsonii bacterial cells for immunoelectron microscopy. These cells hate PBS. My question is: is there other buffers I can use to process this sample that don't interfere with antigenicity? Thanks Login Host: 128.227.146.124 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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On Fri, Feb 28, 2020 at 9:38 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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Email: ralvaradojr-at-ufl.edu {mailto:ralvaradojr-at-ufl.edu} Name: Rudy Organization: University of Florida
Title-Subject: [Filtered] Buffers other than PBS to use for IEM
Message: Hi everyone, I am trying to process a strain of Lactobacillus johnsonii bacterial cells for immunoelectron microscopy. These cells hate PBS. My question is: is there other buffers I can use to process this sample that don't interfere with antigenicity? Thanks Login Host: 128.227.146.124 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
********************************************** Geoff McAuliffe, Ph.D. Assistant Professor Director, Electron Microscopy Core Department of Neuroscience and Cell Biology Rutgers, Robert Wood Johnson Medical School 675 Hoes Lane West, Piscataway, NJ 08854 voice: (732) 235-4583 mcauliff-at-rwjms.rutgers.edu **********************************************
I suggest to process in a buffer that is compatible with your bacterial strain. Buffers in general will not have a negative effect on antigenicity. I mainly used 100mM PB and PHEM buffers in the past, when processing cells in culture.
Are you going to chemically fix your specimen with aldehydes? Do you intend to embed in a plastic? Choose/work out a fixation method and embedding method compatible with the characteristics of the primary antibody. You may test the effect of fixation on signal intensity already on the LM level using an immunofluorescent method or combine immunogold labeling with silver enhancement.
During the actual immuno incubation it will be beneficial to use a buffer with sodium chloride. In general PBS, pH 7.4 is a good option. TBS is an alternative. The buffer capacity of Tris at pH 7.4 is however suboptimal. You may go up to a higher pH, e.g., pH 8.2 depending on the additional additives you use to diminish background staining. I recommend https://aurion.nl/sharing-our-knowledge/newsletters-and-newsflyers/newsletter-1-background-suppression/for additional information.
Kind regards,
Peter van de Plas
} On 28 Feb 2020, at 01:39, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } X-from: shakeel waqqar {shakeelwaqqar-at-gmail.com {mailto:shakeelwaqqar-at-gmail.com} } } } } Try Normal Saline } } On Fri, Feb 28, 2020 at 9:38 AM {microscopy.listserver-at-gmail.com } {mailto:microscopy.listserver-at-gmail.com} } {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America } To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver } On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } X-from: ralvaradojr-at-ufl.edu {mailto:ralvaradojr-at-ufl.edu} {mailto:ralvaradojr-at-ufl.edu} } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both ralvaradojr-at-ufl.edu {mailto:ralvaradojr-at-ufl.edu} } {mailto:ralvaradojr-at-ufl.edu} , } Microscopy-at-Microscopy.com {mailto:Microscopy-at-Microscopy.com} so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } } --------------------------------------------------------------------------- } } Email: ralvaradojr-at-ufl.edu {mailto:ralvaradojr-at-ufl.edu} {mailto:ralvaradojr-at-ufl.edu} } Name: Rudy } Organization: University of Florida } } Title-Subject: [Filtered] Buffers other than PBS to use for IEM } } Message: Hi everyone, } I am trying to process a strain of Lactobacillus johnsonii bacterial } cells for immunoelectron microscopy. These cells hate PBS. 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Peter van de Plas Aurion Binnenhaven 5 Wageningen 6709 PD The Netherlands Phone: +31 317 415094 http://www.aurion.nl
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Email: rvancamp-at-kettering.edu
Name: Rick Van Camp
Organization: Kettering University
Title-Subject: [Filtered] Printing tiff images
Message: I would like to display some of the tiff images that have been collected with our TEM in the hall outside the lab. Can anyone advise me on what properties a given printer needs to possess such that these images retain the high quality they inherently possess? The idea is to leave a positive impression on those people that walk by the lab.
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Dear Rick, Unfortunately I don’t have practical experience with PINTING TIFF files properly…don’t own a TIFF-printable printer (☺)
But: Information is available: e.g. https://itstillworks.com/print-large-tiff-file-6469452.html
Copy & Paste: Just for convenience of all Readers/colleagues eventually not being able to access the given URL: QUOTE: How to Print a Large Tiff File Step 1 Select a paper that is suited to print photographic images. Using plain typing paper or copy paper will not capture the range of values and colors inherent in a high-quality Tiff file. If possible, select the same type of paper as the manufacturer of your printer. Step 2 Access a printer that is designed to Tiff quality images, if possible. Most home inkjet printers will print a photographic image of an acceptable quality, though photo-specific printers will give the contrast and color range that a Tiff is designed to provide. These types of printers often have the word "photo" in their model name, and use individual-color ink cartridges. Step 3 Clean the print heads on your printer. This prevents lines, known as banding, as well as color shifts. Right-click on the printer icon in the taskbar, located on the lower right corner of your screen. Follow the step-by-step instructions after clicking the option to clean the print heads. Step 4 Open the Tiff file in your computer's imaging software or default picture viewer. Make any changes to contrast, color and cropping as necessary or possible. Step 5 Select Print from the File menu in the software. Select the option called Page Options or Print Options, depending on your software model. This will bring up a dialog box specific to your printer. Step 6 Choose the paper size, orientation, the source. The size will likely be legal, or 8.5 by 11 inches. The orientation refers to whether the image is tall (portrait) or long (landscape). The source is either a sheet or roll of paper. Step 7 Select the type of media being used. The instruction sheet with your paper may suggest a certain media based on your printer manufacturer. There may be a number of options to experiment with, but the important detail is to select whether the paper you have chosen has a glossy surface or is matte. Step 8 Select the highest print quality available. This option is known under various terms depending on the manufacturer, but will usually be the top or last quality in a list of options. Load the paper into the printer on its correct side as explained by the paper's instruction sheet. Press print. If the print has problems with its contrast or color, return to the file in the imaging software. Make the necessary changes and repeat the process as needed. Tip • So as not to waste ink, use the selector tool in your imaging software to choose a portion of the image with a variety of colors or contrast. Copy and paste this portion into a new document. Make the necessary changes and print test strips until it is determined what changes to apply to the entire image. Items you will need • Computer with imaging software or default picture viewer • Tiff file • Printer, preferably of photographic quality
Elizabeth MOTT, My Printer Is Not Printing TIFF Files https://smallbusiness.chron.com/printer-not-printing-tiff-files-57302.html [ Related Articles, References (3) https://lbis.kenyon.edu/helpline/printers/troubleshooting Microsoft: Description of the Guidelines for Selecting the Appropriate Picture Format in an Office Program Adobe Systems: Developer Resources: TIFF Resources (1) Dux Computer Digest: How to Troubleshoot Printer Problems ] What Is the Best Image Format for Printing? https://www.shutterstock.com/support/article/best-image-format-for-printing
Asked Feb 2019: https://photo.stackexchange.com/questions/104804/jpeg-or-tiff-files-better-for-print-images JPEG or TIFF files better for print images?
Asked August 2007 https://www.photo.net/discuss/threads/is-it-possible-to-print-tiff-files.283228/
Best regards and good luck,
Wolfgang MUSS SALZBURG, AUSTRIA
========================================================= MUSS Wolfgang Dr. phil. / PhD - retired Ignaz-Rieder-Kai 19/6 A-5020 SALZBURG Österreich-AUSTRIA Mobile-Tel.: 0043(0)676 5 369 456 E-mail: wij.muss-at-aon.at E-Mail altern.: womuss-at-gmail.com FRMS, Retired Member of MSA & other (Inter-)National Societies Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS inviting you to join RG (Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html, and join 15+ million researchers, including 63 Nobel Laureates) „ResearchGate is the professional network for scientists and researchers. Over 15 million members from all over the world use it to share, discover, and discuss research. We're guided by our mission to connect the world of science and make research open to all.“
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If you are interested in printing out your images with your printer. Make sure that your printer has the capabilities to print on photo paper and what type of ink it uses. If you check the name/version of your printer you can look up to see what your printer can and cannot do for printing qualities. When you do print be sure to make sure that the file format/ size of image you have. Due to when you print out your image and you manipulate it may warp the image. Here are some links to help you. -Julie
Link best printer for photos } } https://www.bestproducts.com/tech/electronics/a26933257/top-rated-photo-printers/?src=arb_ga_bp_m_bm_a26933257&utm_source=google&utm_medium=cpc&utm_campaign=arb_ga_bp_m_bm_a26933257&gclid=EAIaIQobChMIgPyip-CE6AIVkspkCh1Mowy8EAAYASAAEgJGa_D_BwE
Why do pictures look different when I print them link } } https://www.google.com/amp/s/www.howtogeek.com/397798/why-do-photos-look-different-when-i-print-them/amp/
Sent from my iPhone
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Can anyone advise me on what properties a given printer needs to possess } such that these images retain the high quality they inherently possess? 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1) Epson Photo printer with Photo inks, 6 to 9 color (the blue ink is used in B&W printing). But! Don't buy one unless you will be printing at least once a month, maybe even once a week. Inks are hideously expensive and 3rd party inks don't work well or at all (inks are bar coded so a printer company can prevent other brands or refills from being used). However. Unless you do print something, even a test page, every so often, the ink dries, clogs a valve or three, and this cannot be fixed. The printer is trash. So, if you just want a set of images for the hallway, use the campus printing service or a business.
2) High quality photo paper. Let the ink completely dry (it sits on top of the coating, hence higher resolution printing) for 30-60 minutes. *Laminate* the print. This seals out oxygen, which fades images.
3) When moving the image files, do *not* use drag-and-drop, use copy/paste. Drag and drop in Windows can change the image resolution to the monitor's resolution. This was true, I do not know if it is still true with Windows 10 (or 7), but I don't trust it. I don't know about Linux, and I don't think MacOS does this. But copy/paste is easy.
4) Not what you asked, but more of a tech-looking thing that makes admin types happy: Put up a display monitor or three in the hallway, and set up a computer to send images to the monitors. No printer, no buying expensive inks, no worrying about file types and sizes, easier to change images, etc. You can maybe get the monitors and computer from campus IT - ones that have been replaced with something newer, but are still perfectly good and likely free.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office
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We have a job vacancy for a permanent position, part time, technician in our microscopy/microanalysis laboratory here at AgResearch Institute in New Zealand.
We are primarily working on biological and bio-based materials using SEM, TEM and other similar methods. We are looking to hire a technician to carry out a range of tasks from assisting with sample preparation to helping look after the instrumentation and do some troubleshooting etc. The role would suit someone with a good working knowledge with instrumentation, an eye for detail and good collaboration skills.
For more details look under AgResearch on the Science New Zealand website under careers for more details.
*AgResearch Limited | *Lincoln Research Centre *| New Zealand* Dr Duane P Harland, Senior Scientist *T*+64 3 321 8710 *E*duane.harland-at-agresearch.co.nz {mailto:duane.harland-at-agresearch.co.nz}
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Title-Subject: [Filtered] We are hiring! Electron Microscopist / Imaging Specialist
Message: Hello! Sivananthan Laboratories, Inc. is a high-tech business incubator focused on promoting economic growth in Illinois and the United States through fostering cutting-edge, fundamental research and development. We work on R&D for AI, infrared detectors, image processing, solar energy, and more. We are currently looking for a successful candidate who is skilled in all aspects on scanning electron microscopy (SEM), transmission electron microscopy (TEM) and scanning transmission electron microscopy (STEM) with experience in image simulation and state-of-the-art processing and analytics packages. If you are interested, please go to this link (http://sivananthanlabs.us/employment/) and apply for this position!
If you have any questions, feel free to email me at mrajasingham-at-sivananthanlabs.us
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One of my users wants to replace a tungsten filament with aLaB6tip in my FEI BioTwin12 for screening/imaging cryoEM samples. I know aLaB6is much more expensive. But regardless the cost, what are other downside impact on my other users who primarily use the scope for imaging plastic sections, negative-stained samples? Thank you!
We're about to the same in our Tecnai Spirit Biotwin T12. The FEI/Thermofisher guys have no concern about that.
I've used a T12 in CMM at the University of Queensland, Brisbane/Australia that has got a LaB6.
You must clean the gun assembly and the Wehnelt as well in order to get rid of W residues before.
Although a LaB6 is more expensive than a W filament, its life time is much longer. It might last over 1000 ours. It lasts 2000 hours in average on our Tecnai T20. You know it will give you a brighter beam, and its energy spread is lower.
Regarding the other users, they will be quite happy when looking at their plastic sections and negative staining, not only the cryo-EM users.
It's always a good practice to set a lower C2 aperture and play around with the spot size to a suitable electron dose for each sample. I know some users don't like to change the apertures and do the alignments but they should get use of it.
My only concern is about the gun preassure. As the Tecnai Spirit Biotwin T12 does have a dedicated IGP for the gun (ours doesn't have), the vacuum in the gun is broken down every time you run the cryo cycle. So the LaB6 cathode gets contaminated. But I still think the benefits of the LaB6 will give you are worth to have it on your T12.
Best wishes,
On Sat, 7 Mar 2020, 16:56 , {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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X-from: Ning, Gang {gxn7-at-psu.edu {mailto:gxn7-at-psu.edu} }
Dear Listers,
One of my users wants to replace a tungsten filament with aLaB6tip in my FEI BioTwin12 for screening/imaging cryoEM samples. I know aLaB6is much more expensive. But regardless the cost, what are other downside impact on my other users who primarily use the scope for imaging plastic sections, negative-stained samples? Thank you!
if your FEI TEM is equipped with a getter pump and does reach a sufficient vacuum value you will generally benefit from switching to LaB6 cathodes. An LaB6 cathode can be pre-mounted in a spare wehnelt and interchanged within an hour, if the vacuum system in the rest of the scope is pumped down to limit. Use dry Nitrogen gas for flooding and let it run until cathode / wehnelt is changed... LaB6 cathode needs to be centred VERY well, within a few 1/100 mm. For accurate height settings see the data sheets at Kimball or DENKA websites.
The spot diameter will be smaller than with Tungsten, the electrons coming from the cathode more monochromatic. Both things will help getting better resolution / better focus and astigmatism corrections in the images.
The luminosity of the gun will be significantly higher; that would help at magnifications more than 50-100 k... But: very thin UM cuts could burn under a concentrated beam.
I suppose the automatic heating regime for the LaB6 cathode could be selected at the TEM setup. It will need slow heating (and cooling), ca. 2 minutes each.
Other than the cathode handling and the costs (but: cathode will run ca. 1000-2000 hours) there is only win-win...
I use LaB6 since a lot of years at a Philips / FEI EM420.
Best,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] lead aspartate as post-stain
Message: Hello everyone, I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate). Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep. If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate any and all information.
THanks! John Shields Univ. of Georgia
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
John,
Lead aspartate? Isn't that used as a sweetner in some of the heavier diet pastries?
Phil ----------------------------------------- Philip Oshel Imaging Center Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 office (989) 774-7567 lab
________________________________________ X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Monday, March 9, 2020 16:08 To: Oshel, Philip Eugene
X-from: jpshield-at-uga.edu
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] lead aspartate as post-stain
Message: Hello everyone, I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate). Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep. If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate any and all information.
THanks! John Shields Univ. of Georgia
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No no no, you're think of sugar of lead or lead acetate. Hey, it worked for the Roman Empire.
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Monday, March 9, 2020 7:19 PM To: Frank Karl {frank_karl-at-ardl.com}
X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
John,
Lead aspartate? Isn't that used as a sweetner in some of the heavier diet pastries?
Phil ----------------------------------------- Philip Oshel Imaging Center Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 office (989) 774-7567 lab
________________________________________ X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Monday, March 9, 2020 16:08 To: Oshel, Philip Eugene
X-from: jpshield-at-uga.edu
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Email: jpshield-at-uga.edu Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] lead aspartate as post-stain
Message: Hello everyone, I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate). Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep. If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate any and all information.
THanks! John Shields Univ. of Georgia
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Oh Right, right, Sorry, I mean the "Stain formerly known as Lead Aspartame". Sheesh John S
On Wed, Mar 11, 2020 at 9:04 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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X-from: Frank Karl {frank_karl-at-ardl.com {mailto:frank_karl-at-ardl.com} }
No no no, you're think of sugar of lead or lead acetate. Hey, it worked for the Roman Empire.
Stay safe...........
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} [mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} ] Sent: Monday, March 9, 2020 7:19 PM To: Frank Karl {frank_karl-at-ardl.com {mailto:frank_karl-at-ardl.com} } Subject: [Microscopy] Fwd: Re: viaWWW: lead aspartate as post-stain
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} }
John,
Lead aspartate? Isn't that used as a sweetner in some of the heavier diet pastries?
Phil ----------------------------------------- Philip Oshel Imaging Center Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 office (989) 774-7567 lab
________________________________________ X-from: microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } Sent: Monday, March 9, 2020 16:08 To: Oshel, Philip Eugene Subject: [Microscopy] viaWWW: lead aspartate as post-stain
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Email: jpshield-at-uga.edu {mailto:jpshield-at-uga.edu} Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] lead aspartate as post-stain
Message: Hello everyone, I have been searching for any work on lead aspartate used as a post-stain (similar to lead citrate). Unfortunately I only pull up its use as an en bloc stain - lately for serial section SEM sample prep. If anyone has literature, or has done some previous work seeing if LA would work, I would appreciate any and all information.
THanks! John Shields Univ. of Georgia
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Email: mbrukman-at-seas.upenn.edu Name: Matthew Brukman
Organization: University of Pennsylvania
Title-Subject: [Filtered] High Vacuum STM available
Message: Good morning!
We have a surplus high vacuum STM system free to a good home. Components include
RHK controller Omicron STM-1 head vacuum chamber PC with RHK software roughing, turbo, and ion pumps frame with air-supported legs
Recipient would be responsible for transport and reassembly. Please contact me with questions.
Regards, Matt
Matthew Brukman Scanning and Local Probe Facilities Director Singh Center for Nanotechnology 3205 Walnut St. Phila., PA 19104 215-746-2373
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Is there a recommended procedure /materials for disinfecting optical microscope eyepieces, optics and surfaces against COVID-19?
Alan Paris Leica Microsystems Inc. alan.paris-at-leica-microsystems.com
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Email: nmz787-at-gmail.com Name: Nathan McCorkle
Organization: Sole proprietor
Title-Subject: [Filtered] How to clean nickel shim of magnetic and or glass particles?
Message: I have a nickel shim destined for nanoimprint lithography, made by electroforming e-beam exposed photoresist. I don't have a proper cleanroom, but I've been trying to strip what seemed like residual ZEP e-beam resist... And it has not been going so well. I've tried acetone, dichloromethane, n-methyl pyrrolidone, and 10% NaOH. Sonicated with heat in both acetone and NaOH (at different times). The NaOH is the most recent attempt, and it seemed to show improvement under FIB imaging, but I also noticed what appeared to be redeposition. I can only imagine this is due to particulate in my solvents, dirty air as I blow dry the shim or carry it from my sonicator to my FIB desk, or maybe insoluble particles like glass or ferromagnetic dust which start to settle onto the sample as soon as the sonicator is turned off.
Features are around 150nm linewidth, high frequency and complex shaped... So lots of small say 500nm sized holes/crevices which I'd been thinking was just diffusion limited for the solvent to get into and do it's work. But now I'm pretty confused.
Should I invest in some .45 and .22 micron syringe filters for all my fluid work? Should I tape a magnet to the outside of the beaker I've been sonicating in, to try and collect such particles? What's a standard semiconductor lab method for cleaning magnetic particles from magnetic layers? How about the idea of insolubles? Or can someone recommend a solution that will etch glass but not nickel?
Thanks, -Nathan
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Research Scientist Materials Research Laboratory University of Illinois at Urbana-Champaign
The Materials Research Laboratory (MRL) at the University of Illinois at Urbana-Champaign is seeking a highly motivated Research Scientist to participate and actively support research in the area of scanning electron microscopy. The Research Scientist will provide comprehensive technical support and user training for equipment, procedures, and safety in the broad areas of materials microanalysis and microfabrication.
The successful candidate will become a member of the dedicated staff of approximately 20 scientists and engineers, who maintain major research instruments in the MRL's core facilities for electron microscopy, soft materials characterization, scanning probe microscopy, laser and optical spectroscopy, surface analysis, x-ray diffraction, and nanofabrication facilities including two cleanrooms. Approximately 1,000 researchers from across our campus as well as other academic institutions, industry, and national laboratories use the facilities, logging more than 100,000 user hours annually. The lab is recognized as one of the premier mid-sized user facilities in the nation. For details, please visit us online at https://mrl.illinois.edu/facilities/.
The University of Illinois is an Equal Opportunity, Affirmative Action employer. Minorities, women, veterans and individuals with disabilities are encouraged to apply. For more information, visit http://go.illinois.edu/EEO.
Responsibilities include: * Prepare and deliver primary and advanced training on the various techniques and instrumentation available in the electron microscopy core, particularly in scanning electron microscopy and related techniques. * Formulate, compile and distribute suitable suggestions for documentation improvements for collective staff scientist review. Incorporate approved modifications into training documents and procedures. This includes the creation of video and multimedia tutorials. * Actively participate in research using electron microscopy techniques in new materials, such as ceramics, metals, semiconductor multilayers and super lattices, polymer and biological materials, etc. * Perform routine preventative maintenance tasks which vary daily, monthly, and annually for assigned laboratory instrumentation. Examples include daily checks to ensure instruments are running correctly; monthly proactive checks to ensure no hazards are present; annual maintenance service on vacuum pumps and supporting mechanical equipment. * Identify hazards and/or potential failure modes by comparing equipment usage and performance to establish safety protocols while conducting user training or performing maintenance. If the equipment is not operating within tolerances or any engineering safety controls are malfunctioning, determine corrective actions to hardware, operating procedures or user training in conjunction with the assigned staff scientist and implement the changes. * Crosstrain in other facility techniques in order to act as backup for other staff members and increase knowledge and experience. * Give oral presentations to large audiences, including recording training material for media outlets, and online, live video streaming. * Perform facility-wide safety tasks as assigned. * Conduct department or campus specified lab inspections for assigned operating areas and participate in reviews of non-assigned areas as requested. * Assume additional responsibilities to promote the unit's mission as needed.
Minimum Qualifications * Ph.D. degree in engineering, chemical, physical sciences or related field. * Two years of hands-on experience in the following areas: * operation of scanning electron microscopes, including detailed knowledge of main physical principles, concepts and applications of electron microscopy; * training researchers in the use of scanning electron microscopes including data interpretation on related techniques; * troubleshooting, preventive maintenance and routine repair of scanning electron microscopes; * use of advanced analytical techniques such as energy-dispersive X-ray spectrometry, cathodoluminescence imaging and spectrometry, and electron backscatter diffraction using the scanning electron microscope. * sample preparation techniques for electron microscopy including polishing, coating, milling, etc. * Three years of instructional/training experience delivering technical information. * Previous experience in creating/developing instructional, instrument operation and training material in electron microscopy. * Excellent oral and written communication skills.
Preferred Qualifications * Experience working in a multi-user academic research facility. * Post-doctoral experience in engineering, chemical, physical sciences or related field. * Chemistry background necessary for identifying chemicals for waste processing. * Experience with operation of focused ion beam (FIB/SEM) systems and transmission electron microscopes and related techniques.
This is a full-time, benefits-eligible academic professional position appointed on a 12-month service basis. The expected start date is as soon as possible. Salary is commensurate with experience and qualifications. To apply, please complete a candidate profile at http://jobs.illinois.edu and upload the following as a single file: a cover letter, resume, and the names and contact information for three professional references. To ensure full consideration, all requested information must be submitted by April 2, 2020.
For further information regarding application procedures, contact Summer Redman at mailto:sredman-at-illinois.edu or 217-300-5400.
The University of Illinois conducts criminal background checks on all job candidates upon acceptance of a contingent offer. As a qualifying federal contractor, the University of Illinois System uses E-Verify to verify employment eligibility. The University of Illinois must also comply with applicable federal export control laws and regulations and, as such, reserves the right to employ restricted party screening procedures for applicants.
________________________________________ James C Mabon, Ph.D. Senior Research Scientist University of Illinois at Urbana-Champaign Materials Research Laboratory 104 S. Goodwin Ave. Urbana, Illinois, 61801 ________________________________________
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Email: Stephen.Beck-at-ncc.edu Name: Steve Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Teaching SEM Online
Message: Dear Colleagues, I am teaching my SEM course this semester and like many institutions, we are trying to teach online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online, however, after that we need to get on the SEM to image the required samples. Does anyone have any ideas regarding teaching SEM online? I have informed my administration that this is impossible - I haven't received the courtesy of a response yet!
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It is very challenging situation, but everything depends on what generation of hardware you have in the lab. Depends on who is your vendor, it may be possible to share the screens and allow users to move the mouse and type on the screen. Such things exist for the troubleshooting on most of the modern instruments. Technically, some instruments can be even used remotely for a high quality work. The only bottleneck I'd - someone needs to.prepare the sample and mount it inside the analytical tool. As the instruments get more and more sophisticated and automated - that approach may become more popular anyway.
Ask your vendor - they may already have the answer for you.
Stay healthy!
Lolita 8:45 AM, March 21, 2020, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} :
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Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Teaching SEM Online
Message: Dear Colleagues, I am teaching my SEM course this semester and like many institutions, we are trying to teach online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online, however, after that we need to get on the SEM to image the required samples. Does anyone have any ideas regarding teaching SEM online? I have informed my administration that this is impossible - I haven't received the courtesy of a response yet!
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We have a couple of our SEMs connected to the internet for remote access. Through this interface, the microscope is completely controllable from your own PC. We can load your samples and then you or your students would be able to take control of the instrument and practice from your own homes.
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Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Teaching SEM Online
Message: Dear Colleagues, I am teaching my SEM course this semester and like many institutions, we are trying to teach online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online, however, after that we need to get on the SEM to image the required samples. Does anyone have any ideas regarding teaching SEM online? I have informed my administration that this is impossible - I haven't received the courtesy of a response yet!
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did you try using a plasma cleaner for cleaning the surfaces and also a plasma cleaner like the Evactron at the FIB chamber to keep the specimen clean during scanning?
If you can mount the specimen with the surface to be cleaned facing down to the bottom of the beaker you might get rid of deposits coming from above ;-)
Another try to clean the surfaces might be to plunge it in liquid nitrogen or to use a vacuum chamber with the cleaning solution and pump to a level below sublimation.
And sure: clean micro-filtered solutions would help.
Nickel and magnetism: you could use a demagnetizer to decrease / erase the magnetism in the shim first.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 21.03.20 um 14:55 schrieb microscopy.listserver-at-gmail.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: nmz787-at-gmail.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } nmz787-at-gmail.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: nmz787-at-gmail.com Name: Nathan McCorkle } } Organization: Sole proprietor } } Title-Subject: [Filtered] How to clean nickel shim of magnetic and or glass particles? } } Message: I have a nickel shim destined for nanoimprint lithography, made by electroforming e-beam } exposed photoresist. I don't have a proper cleanroom, but I've been trying to strip what seemed like } residual ZEP e-beam resist... And it has not been going so well. I've tried acetone, } dichloromethane, n-methyl pyrrolidone, and 10% NaOH. Sonicated with heat in both acetone and NaOH } (at different times). The NaOH is the most recent attempt, and it seemed to show improvement under } FIB imaging, but I also noticed what appeared to be redeposition. I can only imagine this is due to } particulate in my solvents, dirty air as I blow dry the shim or carry it from my sonicator to my FIB } desk, or maybe insoluble particles like glass or ferromagnetic dust which start to settle onto the } sample as soon as the sonicator is turned off. } } Features are around 150nm linewidth, high frequency and complex shaped... So lots of small say 500nm } sized holes/crevices which I'd been thinking was just diffusion limited for the solvent to get into } and do it's work. But now I'm pretty confused. } } Should I invest in some .45 and .22 micron syringe filters for all my fluid work? Should I tape a } magnet to the outside of the beaker I've been sonicating in, to try and collect such particles? } What's a standard semiconductor lab method for cleaning magnetic particles from magnetic layers? How } about the idea of insolubles? Or can someone recommend a solution that will etch glass but not nickel? } } Thanks, } -Nathan } } Login Host: 50.39.163.117 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 11, 53 -- From microscopy.listserver-at-gmail.com Sat Mar 21 08:47:00 2020 } 11, 53 -- Received: from mail-il1-f182.google.com (mail-il1-f182.google.com [209.85.166.182]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 02LDl0KO030119 } 11, 53 -- for {microscopy-at-microscopy.com} ; Sat, 21 Mar 2020 08:47:00 -0500 } 11, 53 -- Received: by mail-il1-f182.google.com with SMTP id h3so8517536ils.3 } 11, 53 -- for {microscopy-at-microscopy.com} ; Sat, 21 Mar 2020 05:46:01 -0700 (PDT) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=mH3hQX0JfJ+qEqtaq+MXuNo/ABuFHuYdS+6Lkr4Y6yM=; } 11, 53 -- b=dnnl/o8YK+SszO3Nm5rJjrGFzXDjuoO5012DEK5glVzMak6UpaVSkXllHz30ah4dAv } 11, 53 -- G66sme2fXNthcQ5O0XdjTmWqGVc+8VxfFN2cxlHCFB7a5Y67E3B/w+3vpW6QbV/OwjOZ } 11, 53 -- 17vqHlxRNI9UvrRRGHmLRqdIEmKtMIACWFkjZ7dqR5lH2w8+kgOR/T5RBDcKpXr3InG1 } 11, 53 -- qGJIMRf+jMmpAvUQYDZitQaKgtWxAFM8FdCGKxj796bg0FDI72jIjozC+3N/Go1W+Wnk } 11, 53 -- VnbVRHvSoeU2k0gYmIseWSVfZbh8soOB3Udj+YGWxDkkDOwyfwKKN0lo+N3MmcAISK7A } 11, 53 -- UCIw== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=mH3hQX0JfJ+qEqtaq+MXuNo/ABuFHuYdS+6Lkr4Y6yM=; } 11, 53 -- b=hXZas+rlSuV0lba6didsrmxBAeXacuYQe7mUNe4DfyHww5h2MmRBFx0D+Sb4ItbEBD } 11, 53 -- joqvretzWGXjPstPy5pGe5fkSGqV17N1rrESsiNMPm3Z0Yuruz973pMxNcA+ReBe6SDQ } 11, 53 -- z69BxT6UV6CU0GkO3YZFe9r9c2b5gsqWlJwpb8JbrqZyh4Zs9zsUwt2ARArCxEhVrCLq } 11, 53 -- 10UrcVCj9xnsslxdB/kPKxC2vQ6J7x6X03H6PJstFULA/WAQdGvjmFnRF0BWIPAyyxY2 } 11, 53 -- EelGn5xXqZWbv5OKiNIaGAasJFtajGL2cwkcwDe8D4BOYFePF48Y3pGZHUKM1S8KxU15 } 11, 53 -- J7RA== } 11, 53 -- X-Gm-Message-State: ANhLgQ0adY4veLh0VKZn/9UFDOB+linnE0u8xD34kP+ALeXDuI6InkR/ } 11, 53 -- lcMX8MnBbO+Fm3L6zZxcNLs3iwoe } 11, 53 -- X-Google-Smtp-Source: ADFU+vttqwpYeeGgojuWRhepav+vjsfSWPwdoBoZ4wOHhalNwUAOzE60ZtLJhw72LbYEdbjDf2RflQ== } 11, 53 -- X-Received: by 2002:a92:6b06:: with SMTP id g6mr12985242ilc.84.1584794761288; } 11, 53 -- Sat, 21 Mar 2020 05:46:01 -0700 (PDT) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:8069:12b3:af30:117f]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id 27sm3107763ilv.75.2020.03.21.05.46.00 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 11, 53 -- Sat, 21 Mar 2020 05:46:01 -0700 (PDT) } 11, 53 -- Subject: viaWWW: How to clean nickel shim of magnetic and or glass particles? 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12, 29 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , nmz787-at-gmail.com 12, 29 -- References: {202003211355.02LDtIt2008929-at-microscopy.com} 12, 29 -- From: stefan diller {diller-at-stefan-diller.com} 12, 29 -- Message-ID: {4488c29b-7c90-12c6-6981-c8e1f6800948-at-stefan-diller.com} 12, 29 -- Date: Sat, 21 Mar 2020 17:59:08 +0100 12, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:68.0) 12, 29 -- Gecko/20100101 Thunderbird/68.6.0 12, 29 -- MIME-Version: 1.0 12, 29 -- In-Reply-To: {202003211355.02LDtIt2008929-at-microscopy.com} 12, 29 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 29 -- Content-Transfer-Encoding: 7bit 12, 29 -- Content-Language: en-GB 12, 29 -- X-Envelope-From: {diller-at-stefan-diller.com} 12, 29 -- X-Scanned-By: rockenstein AG ==============================End of - Headers==============================
if nobody can help you nearby and as long as Corona will stay outside my lab we can try set something as a life conference from my lab (I might need some help for this, so it might take some time to set it up...).
I am using a FE-SEM TESCAN MIRA3 and a Philips / FEI TEM EM420.
Some nice resources can be found by searching at google for "teaching electron microscopy"
and here
https://www.fei.com/education-resources/
For some outreach to your students you can use my nanoflight channel at vimeo to show what is possible with electron microscopy and a lot of effort.
https://vimeo.com/channels/nanoflight
and if you like I can put some more 3D nanoflights online for the OCULUS or other 3D glasses.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
To clean a surface of particulates, I would use replicating tape. This is a cellulose acetate tape (non-adhesive) that you soften with acetone and press down onto the surface. Let it dry and peel it off. All the particulates should come with it. I've had better luck in removing particulates this way compared with ultrasonics, rinses, etc.
I'm not sure if an adhesive tape will work but if you don't have replicating tape, you might try some of the tape with the "Post-it" type adhesive. It may take several applications to remove everything. Replicating tape is available from most of the EM supply houses. It comes in both a thick and thin form. Cheers, Henk
----------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Saturday, March 21, 2020 9:53 AM To: Colijn, Hendrik {colijn.1-at-osu.edu}
Hi Stephen,
Depending on the size of your class - you can use one of remote desktop packages, such as Teamviwer, VNC, RDP, Chrome remote desktop, etc... to let your students see (and even operate) GUI of the SEM, and hear your explanations.
I've been using TeamViewer for remote troubleshooting and training of FIB operators a couple of years, although haven't tried it in "classroom" kind of approach.
Best Wishes, Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 3/21/2020 9:43 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Stephen.Beck-at-ncc.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } Stephen.Beck-at-ncc.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: Stephen.Beck-at-ncc.edu Name: Steve Beck } } Organization: Nassau Community College } } Title-Subject: [Filtered] Teaching SEM Online } } Message: Dear Colleagues, } I am teaching my SEM course this semester and like many institutions, we are trying to teach } online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online, } however, after that we need to get on the SEM to image the required samples. } Does anyone have any ideas regarding teaching SEM online? I have informed my administration that } this is impossible - I haven't received the courtesy of a response yet! } } Thanks! } Login Host: 173.77.159.14 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 8, 53 -- From microscopy.listserver-at-gmail.com Sat Mar 21 08:42:55 2020 } 8, 53 -- Received: from mail-io1-f52.google.com (mail-io1-f52.google.com [209.85.166.52]) } 8, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 02LDgtJo026786 } 8, 53 -- for {microscopy-at-microscopy.com} ; Sat, 21 Mar 2020 08:42:55 -0500 } 8, 53 -- Received: by mail-io1-f52.google.com with SMTP id h131so9143628iof.1 } 8, 53 -- for {microscopy-at-microscopy.com} ; Sat, 21 Mar 2020 05:41:56 -0700 (PDT) } 8, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=gmail.com; s=20161025; } 8, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 8, 53 -- :in-reply-to:content-language:content-transfer-encoding; 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X-from: David Huskisson {david-at-emanalytical.co.uk}
Hi Stephen,
We have a couple of our SEMs connected to the internet for remote access. Through this interface, the microscope is completely controllable from your own PC. We can load your samples and then you or your students would be able to take control of the instrument and practice from your own homes.
This message is private and confidential. If received in error, please destroy and notify sender. Sender does not intend to waive confidentiality or privilege. Dissemination, use of or reliance upon this email is prohibited when received in error. Email may be susceptible to data corruption, interception and unauthorised amendment, and no liability is accepted by the sender for any of the foregoing. It is the recipient’s responsibility to scan the email and any attachment for viruses.
On Sat, Mar 21, 2020 at 1:48 PM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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Email: Stephen.Beck-at-ncc.edu {mailto:Stephen.Beck-at-ncc.edu} Name: Steve Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Teaching SEM Online
Message: Dear Colleagues, I am teaching my SEM course this semester and like many institutions, we are trying to teach online/remotely as our campus is closed due to COVID-19. I have administered my midterm exam online, however, after that we need to get on the SEM to image the required samples. Does anyone have any ideas regarding teaching SEM online? I have informed my administration that this is impossible - I haven't received the courtesy of a response yet!
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From kelnanc57ctyee-at-gmail.com Sat Mar 28 20:49:33 2020 Return-Path: {kelnanc57ctyee-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [123.11.215.48] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 02T1nWc6020483 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 28 Mar 2020 20:49:33 -0500 Message-ID: {4660B6DC.5BA45810-at-gmail.com}
X-from: Erico Freitas {ericotadeu-at-ufmg.br}
Dear all,
I'm just forwarding you the following TEM/AFM Researcher position (191486) at CNPEM.
The CNPEM is located in Campinas, S=C3=A3o Paulo state, Brazil.
This position is likely for the LNNano (Brazilian National Nanotechnology Laboratory) at CNPEM (https://lnnano.cnpem.br/)
Cheers,
-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
This position is likely for the LNNano (Brazilian National Nanotechnology Laboratory) at CNPEM (https://lnnano.cnpem.br/)
Cheers,
-- Erico Freitas
Physicist/Microscopist at Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Coordinator:Transmission Electron Microscopy laboratory
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Email: phillipa.timmins-at-aurox.co.uk
Name: Phillipa Timmins
Organization: Aurox Ltd
Title-Subject: [Filtered] Aurox On-line Conference on Microscopy 7th and 8th April 2020
Message: Dear All,
I hope you are all staying safe in these troubling and turbulent times. We send out our best wishes from Aurox.
We have had a hugely positive response to our upcoming On-line Conference on Microscopy on 7th and 8th April. We have a fantastic line up of speakers from academia and industry covering a range of topics including: Raman Microscopy, Optical Diffraction Tomography, Cryo-microscopy, Super resolution, Live cell imaging, AI, Image processing, Adaptive Optics, Mesolens, Expansion Microscopy as well as keynote by Tony Wilson and updates from various companies.
I just wanted to let you know that the deadline to register your interest in attending is this Friday at 2PM UK time.
It is free to attend and you can find the program and sign up page here:
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Email: xbren-at-uw.edu Name: Shirley
Organization: UWMC
Title-Subject: [Filtered] Clinical EM lab
Message: Hello colleagues, Does anyone know how many clinical EM labs are in America? our department manager would like to know, and may need help in the future.
Thank you,
Shirley
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From andralbi54nuxdo-at-gmail.com Sat Apr 4 22:18:40 2020 Return-Path: {andralbi54nuxdo-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [123.11.215.6] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 0353IdLn030280 for {microscopylistserverarchive7-at-microscopy.com} ; Sat, 4 Apr 2020 22:18:40 -0500 Message-ID: {413101d60ab5$fbf3b720$ce8d52e7-at-andralbi54nuxdo}
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Email: xbren-at-uw.edu Name: Shirley
Organization: UWMC
Title-Subject: [Filtered] Clinical EM lab
Message: Hello colleagues, Does anyone know how many clinical EM labs are in America? our department manager would like to know, and may need help in the future.
Thank you,
Shirley
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both leroy-at-icmpe.cnrs.fr, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: leroy-at-icmpe.cnrs.fr
Name: Eric Leroy
Organization: CNRS
Title-Subject: [Filtered] Vintage DTSA
Message: Hi, I am searching for the old DTSA software. The web page of the NIST still exists but all the download links are dead. Does anybody have a backup of DTSA 2.5 or 3.1 ? Thank you by advance, Best regards, Eric
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From solizbula031axig-at-gmail.com Wed Apr 8 17:16:46 2020 Return-Path: {solizbula031axig-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [123.11.215.50] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 038MGh6J023039 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 8 Apr 2020 17:16:45 -0500 Message-ID: {A16504D1.F1B47E53-at-gmail.com}
Hello Eric,
if you still need it: I found sea.hqx archive files of PowerDTSAv2.5.1 and PPC_DTSA301 on my computer.
Also the manual.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Email: srousselle-at-stagebio.com
Name: Serge Rousselle
Organization: StageBio
Title-Subject: [Filtered] SEM sample prep: Critical Point Drying and Sputter Coating
Message: 1) Looking for drying chamber and sputter coating equipment for SEM sample preparation that can accommodate large specimens (up to 5cm in diameter or up to 8-10cm long with a narrower diameter such as a large vessel). What equipment would you recommen?
2) Anyone able to provide remote/web-based training for SEM sample preparation (not imaging, just sample prep)? Thank you Serge
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I am a materials scientist and not a biologist, but I will offer some ideas.
I have only seen a couple CPD systems. I don't think they would have been able to accommodate such a large sample. There may be some that could, but I think they would be rare. I recall you will also have issues with thickness. The processing time will depend on the thickness and if all the alcohol is not removed, you will disrupt your structure.
We purchased a Denton sputter coater with a turntable designed to evenly coat samples up to 6 inches in diameter. It worked, but was slower than the standard coater. There was a sectored aperture in front of the target to ensure even coating and that slowed the rate per unit area, and then there was much more area to cover so more time was required.
There may also be issues with the SEM. Our FEI Quanta is the 250 model with a 2-inch stage. Our low-mag limit is 50x at 10 mm working distance, so we can only image 2mm x 1.5mm at a time. We can only survey a 2-inch x 2-inch area at a time. We can load larger samples, but we would have to remove and reset them in order to cover the entire area.
I think you will also find that you may not have good coating over the entire surface. It depends on how convoluted your sample is. You may need to ground the surface in many places to make sure the charge can bleed away to the stage.
With all those issues, I wonder if you might be better off to prepare multiple, smaller samples that can be dehydrated, coated, and imaged using readily available equipment. Hopefully the features of interest would not fall only on the cut lines between the sub-samples.
Also, do you need high magnification to look at small surface structures? If not, the variable pressure or environmental modes of an SEM like the Quanta might not require any sample preparation. It depends on what you want to see.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, April 09, 2020 6:35 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
X-from: srousselle-at-stagebio.com
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Email: srousselle-at-stagebio.com
Name: Serge Rousselle
Organization: StageBio
Title-Subject: [Filtered] SEM sample prep: Critical Point Drying and Sputter Coating
Message: 1) Looking for drying chamber and sputter coating equipment for SEM sample preparation that can accommodate large specimens (up to 5cm in diameter or up to 8-10cm long with a narrower diameter such as a large vessel). What equipment would you recommen?
2) Anyone able to provide remote/web-based training for SEM sample preparation (not imaging, just sample prep)? Thank you Serge
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From smithejoseph18faoog-at-gmail.com Thu Apr 9 17:47:00 2020 Return-Path: {smithejoseph18faoog-at-gmail.com} Received: from gmail.com (hn.kd.ny.adsl [123.11.215.30] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 039Mk2YZ002860 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 9 Apr 2020 17:46:57 -0500 Received: from unknown (131.27.23.190) by mx03.listsystemsf.net with NNFMP; Thu, 09 Apr 2020 17:34:24 -0500 Received: from smtp-server1.cfdenselr.com ([Thu, 09 Apr 2020 17:30:53 -0500]) by mail.naihautsui.co.kr with SMTP; Thu, 09 Apr 2020 17:30:53 -0500 Received: from [76.3.117.196] by mmx09.tilkbans.com with QMQP; Thu, 09 Apr 2020 17:16:38 -0500 Received: from external.newsubdomain.com ([116.202.95.28]) by mts.locks.grgtween.net with QMQP; Thu, 09 Apr 2020 17:15:01 -0500 Message-ID: {564FB272.034354B5-at-gmail.com}
X-from: Desert Rat {desertrat99-at-verizon.net}
Hi folks,
I have a friend who is trying to identify the features in some SEM images but they are bio and I am hard materials. The images are at the following link https://drive.google.com/drive/folders/1vzKbmfoDZT6f9fsJduTgxx_hwKj1-sGE?usp=sharing They were collected from a white microbial mat in a cave at Lava Beds National Monument with NPS permission. They were mounted to SEM stubs with carbon tape and dried in a vacuum oven. Then coated in gold/palladium and put in the SEM. They think the big, 100 micron, thing is a mite? Any other idea welcome. What they really need to know is what are the little wormy things that look like chains of balls |--500nm in diameter? They think they might be bacteria eating the dead mite?
Nick
--
Dr Nicholas Seaton
SEM, FIB & LM Specialist Department Safety Officer Characterization Facility
College of Science and Engineering
University of Minnesota
12 Shepherd Labs
100 Union Street SE
Minneapolis
MN, 55455
email: seato008-at-umn.edu
phone: 612-626-5314
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I am also a materials guy rather than a biologist.
I am inclined to agree with your identification of a mite of some kind.
I don't think the semicircular features are bacteria. The size may be about right, but I don't think the distribution is. I wonder if it might be some sort of exude from the drying process.
I think the very fine structures in the 3500x and 12,000x images are coating grains. I have seen similar things before where the coating does not "like" the substrate. It "beads up", if you will and creates those recognizable structures. I wonder what an uncoated version of the sample would look like.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: seato008-at-umn.edu [mailto:seato008-at-umn.edu] Sent: Saturday, April 11, 2020 11:56 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
Hi folks,
I have a friend who is trying to identify the features in some SEM images but they are bio and I am hard materials. The images are at the following link https://drive.google.com/drive/folders/1vzKbmfoDZT6f9fsJduTgxx_hwKj1-sGE?usp=sharing They were collected from a white microbial mat in a cave at Lava Beds National Monument with NPS permission. They were mounted to SEM stubs with carbon tape and dried in a vacuum oven. Then coated in gold/palladium and put in the SEM. They think the big, 100 micron, thing is a mite? Any other idea welcome. What they really need to know is what are the little wormy things that look like chains of balls |--500nm in diameter? They think they might be bacteria eating the dead mite?
Nick
--
Dr Nicholas Seaton
SEM, FIB & LM Specialist Department Safety Officer Characterization Facility
College of Science and Engineering
University of Minnesota
12 Shepherd Labs
100 Union Street SE
Minneapolis
MN, 55455
email: seato008-at-umn.edu
phone: 612-626-5314
==============================Original Headers============================== 16, 54 -- From seato008-at-umn.edu Sat Apr 11 11:54:59 2020 16, 54 -- Received: from mta-p8.oit.umn.edu (mta-p8.oit.umn.edu [134.84.196.208]) 16, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 03BGswrx002108 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 11:54:59 -0500 16, 54 -- Received: from localhost (unknown [127.0.0.1]) 16, 54 -- by mta-p8.oit.umn.edu (Postfix) with ESMTP id 4901HH4WXDz9vKYm 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 16:56:11 +0000 (UTC) 16, 54 -- X-Virus-Scanned: amavisd-new at umn.edu 16, 54 -- Received: from mta-p8.oit.umn.edu ([127.0.0.1]) 16, 54 -- by localhost (mta-p8.oit.umn.edu [127.0.0.1]) (amavisd-new, port 10024) 16, 54 -- with ESMTP id aXkuqK2uE4lh for {Microscopy-at-microscopy.com} ; 16, 54 -- Sat, 11 Apr 2020 11:56:11 -0500 (CDT) 16, 54 -- Received: from mail-io1-f69.google.com (mail-io1-f69.google.com [209.85.166.69]) 16, 54 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 16, 54 -- (No client certificate requested) 16, 54 -- by mta-p8.oit.umn.edu (Postfix) with ESMTPS id 4901HH3R9sz9vKYh 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 11:56:11 -0500 (CDT) 16, 54 -- Received: by mail-io1-f69.google.com with SMTP id a12so5310757ioe.17 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 09:56:11 -0700 (PDT) 16, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 54 -- d=umn.edu; s=google; 16, 54 -- h=mime-version:from:date:message-id:subject:to; 16, 54 -- bh=pNRK4qwo1Kl/3La6hMDxJ7xSbHi5dvlta3VlKU/V+MA=; 16, 54 -- b=p9gHfS1zhZDhn59d83ZJsC9WaEdGXmFPAYTReNCbo9+WW2B2+34E9y+XDJnYTO5CVF 16, 54 -- ieE6nqqD3nERNCVoe5lckTPOiLeGNTK3xORfYMQqwKSvKxh1jpYsydIC1qn0S26daZ1e 16, 54 -- F7G3tnDwIwM6Xt8eJw5wkk6rSYclznMn1IYfp0py3ZIjGAsyjwY9uDJCEkDZ1Sz7XmiP 16, 54 -- KqlKQb6Fxg7FJRSsPyOG+pSqk4BCKtQfOoYV7Yi5pE9hhbzkoGekI5ewhv3ShLcDho2b 16, 54 -- OPDodGIqQ6GVwHw8aNMCZltTr3JADbEISP2eNstVM69Yw+zZlUfGxyQXWwchrZTdY9r4 16, 54 -- cTmg== 16, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 54 -- d=1e100.net; s=20161025; 16, 54 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 16, 54 -- bh=pNRK4qwo1Kl/3La6hMDxJ7xSbHi5dvlta3VlKU/V+MA=; 16, 54 -- b=arGaRjpcZdvMQdGrgMLOiCnbEqhvGNxFbsHM+NePALW1jnGZktAiZYEQ5wjVrWo2uL 16, 54 -- 2cjalp/dY4qRGSNGRGfH4yrpiCTZZcXSxY4sHTuQbV6Eqvx0rDFUzYXu4FM+KVjLJ/pa 16, 54 -- TD7Jx5aqHZuaNNFqNg2KSuyl9uVd84xyI1FallIhccX5DI+Wzho3Y3Dl6fO84X0H9+5B 16, 54 -- oW0SKlb6C1wyPDuFhKvNBNbaKxvd1lT5CuM6KHXrfK+7rrTzkiUuOs9sk6vSfUluo8B5 16, 54 -- KdGt+mCNYF2ZDuEY9+pcxoW38gRZfY84QUuN9Uv/DOA/b7aKWThXiFEzv1f5LxZXza1h 16, 54 -- gMgg== 16, 54 -- X-Gm-Message-State: AGi0PuapHwgIK3MZU0O1DKCkqngfvA5AsGw7OzK553UJODZEbptUWBnu 16, 54 -- q+/mQ2rxQOPrQCrI3IrfUz5nmO0zMwYGrJKwp7IBpJMdsMFQptWqRim8Ph+aWhYvowbFxXx43z1 16, 54 -- q7qTEjMAchZo+FgsvzwKispzSBqnzqwvFVdaq/xAA 16, 54 -- X-Received: by 2002:a05:6602:1da:: with SMTP id w26mr9302826iot.191.1586624170865; 16, 54 -- Sat, 11 Apr 2020 09:56:10 -0700 (PDT) 16, 54 -- X-Google-Smtp-Source: APiQypI5UqP3RYAu1aj6ARd/tbQMtSFoGB5rP1sVnhaMt3kD78qSa7b60yiCUQGh8HZ0wqN3LaFtLVZ7/HJnC86z9fU= 16, 54 -- X-Received: by 2002:a05:6602:1da:: with SMTP id w26mr9302817iot.191.1586624170591; 16, 54 -- Sat, 11 Apr 2020 09:56:10 -0700 (PDT) 16, 54 -- MIME-Version: 1.0 16, 54 -- From: Nick Seaton {seato008-at-umn.edu} 16, 54 -- Date: Sat, 11 Apr 2020 11:55:59 -0500 16, 54 -- Message-ID: {CAMJSFzKnWoBhQP5oft0XZppEOaqR2Jq48SFmSQMZwR2TdSMQKg-at-mail.gmail.com} 16, 54 -- Subject: SEM images of microbes 16, 54 -- To: Microscopy-at-microscopy.com 16, 54 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
Tel/fax +30 210 8957677 mobile +30 6945 107477 www.eikonika.netwww.aim.cat *************************************
-----Original Message----- X-from: wesaia-at-iastate.edu [mailto:wesaia-at-iastate.edu] Sent: Saturday, April 11, 2020 20:43 To: eikonika-at-otenet.gr
I am also a materials guy rather than a biologist.
I am inclined to agree with your identification of a mite of some kind.
I don't think the semicircular features are bacteria. The size may be about right, but I don't think the distribution is. I wonder if it might be some sort of exude from the drying process.
I think the very fine structures in the 3500x and 12,000x images are coating grains. I have seen similar things before where the coating does not "like" the substrate. It "beads up", if you will and creates those recognizable structures. I wonder what an uncoated version of the sample would look like.
Warren Straszheim Materials Analysis and Research Lab Iowa State University
-----Original Message----- X-from: seato008-at-umn.edu [mailto:seato008-at-umn.edu] Sent: Saturday, April 11, 2020 11:56 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
Hi folks,
I have a friend who is trying to identify the features in some SEM images but they are bio and I am hard materials. The images are at the following link https://drive.google.com/drive/folders/1vzKbmfoDZT6f9fsJduTgxx_hwKj1-sGE?usp =sharing They were collected from a white microbial mat in a cave at Lava Beds National Monument with NPS permission. They were mounted to SEM stubs with carbon tape and dried in a vacuum oven. Then coated in gold/palladium and put in the SEM. They think the big, 100 micron, thing is a mite? Any other idea welcome. What they really need to know is what are the little wormy things that look like chains of balls |--500nm in diameter? They think they might be bacteria eating the dead mite?
Nick
--
Dr Nicholas Seaton
SEM, FIB & LM Specialist Department Safety Officer Characterization Facility
College of Science and Engineering
University of Minnesota
12 Shepherd Labs
100 Union Street SE
Minneapolis
MN, 55455
email: seato008-at-umn.edu
phone: 612-626-5314
==============================Original Headers============================== 16, 54 -- From seato008-at-umn.edu Sat Apr 11 11:54:59 2020 16, 54 -- Received: from mta-p8.oit.umn.edu (mta-p8.oit.umn.edu [134.84.196.208]) 16, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 03BGswrx002108 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 11:54:59 -0500 16, 54 -- Received: from localhost (unknown [127.0.0.1]) 16, 54 -- by mta-p8.oit.umn.edu (Postfix) with ESMTP id 4901HH4WXDz9vKYm 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 16:56:11 +0000 (UTC) 16, 54 -- X-Virus-Scanned: amavisd-new at umn.edu 16, 54 -- Received: from mta-p8.oit.umn.edu ([127.0.0.1]) 16, 54 -- by localhost (mta-p8.oit.umn.edu [127.0.0.1]) (amavisd-new, port 10024) 16, 54 -- with ESMTP id aXkuqK2uE4lh for {Microscopy-at-microscopy.com} ; 16, 54 -- Sat, 11 Apr 2020 11:56:11 -0500 (CDT) 16, 54 -- Received: from mail-io1-f69.google.com (mail-io1-f69.google.com [209.85.166.69]) 16, 54 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 16, 54 -- (No client certificate requested) 16, 54 -- by mta-p8.oit.umn.edu (Postfix) with ESMTPS id 4901HH3R9sz9vKYh 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 11:56:11 -0500 (CDT) 16, 54 -- Received: by mail-io1-f69.google.com with SMTP id a12so5310757ioe.17 16, 54 -- for {Microscopy-at-microscopy.com} ; Sat, 11 Apr 2020 09:56:11 -0700 (PDT) 16, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 54 -- d=umn.edu; s=google; 16, 54 -- h=mime-version:from:date:message-id:subject:to; 16, 54 -- bh=pNRK4qwo1Kl/3La6hMDxJ7xSbHi5dvlta3VlKU/V+MA=; 16, 54 -- b=p9gHfS1zhZDhn59d83ZJsC9WaEdGXmFPAYTReNCbo9+WW2B2+34E9y+XDJnYTO5CVF 16, 54 -- ieE6nqqD3nERNCVoe5lckTPOiLeGNTK3xORfYMQqwKSvKxh1jpYsydIC1qn0S26daZ1e 16, 54 -- F7G3tnDwIwM6Xt8eJw5wkk6rSYclznMn1IYfp0py3ZIjGAsyjwY9uDJCEkDZ1Sz7XmiP 16, 54 -- KqlKQb6Fxg7FJRSsPyOG+pSqk4BCKtQfOoYV7Yi5pE9hhbzkoGekI5ewhv3ShLcDho2b 16, 54 -- OPDodGIqQ6GVwHw8aNMCZltTr3JADbEISP2eNstVM69Yw+zZlUfGxyQXWwchrZTdY9r4 16, 54 -- cTmg== 16, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 16, 54 -- d=1e100.net; s=20161025; 16, 54 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 16, 54 -- bh=pNRK4qwo1Kl/3La6hMDxJ7xSbHi5dvlta3VlKU/V+MA=; 16, 54 -- b=arGaRjpcZdvMQdGrgMLOiCnbEqhvGNxFbsHM+NePALW1jnGZktAiZYEQ5wjVrWo2uL 16, 54 -- 2cjalp/dY4qRGSNGRGfH4yrpiCTZZcXSxY4sHTuQbV6Eqvx0rDFUzYXu4FM+KVjLJ/pa 16, 54 -- TD7Jx5aqHZuaNNFqNg2KSuyl9uVd84xyI1FallIhccX5DI+Wzho3Y3Dl6fO84X0H9+5B 16, 54 -- oW0SKlb6C1wyPDuFhKvNBNbaKxvd1lT5CuM6KHXrfK+7rrTzkiUuOs9sk6vSfUluo8B5 16, 54 -- KdGt+mCNYF2ZDuEY9+pcxoW38gRZfY84QUuN9Uv/DOA/b7aKWThXiFEzv1f5LxZXza1h 16, 54 -- gMgg== 16, 54 -- X-Gm-Message-State: AGi0PuapHwgIK3MZU0O1DKCkqngfvA5AsGw7OzK553UJODZEbptUWBnu 16, 54 -- q+/mQ2rxQOPrQCrI3IrfUz5nmO0zMwYGrJKwp7IBpJMdsMFQptWqRim8Ph+aWhYvowbFxXx43z1 16, 54 -- q7qTEjMAchZo+FgsvzwKispzSBqnzqwvFVdaq/xAA 16, 54 -- X-Received: by 2002:a05:6602:1da:: with SMTP id w26mr9302826iot.191.1586624170865; 16, 54 -- Sat, 11 Apr 2020 09:56:10 -0700 (PDT) 16, 54 -- X-Google-Smtp-Source: APiQypI5UqP3RYAu1aj6ARd/tbQMtSFoGB5rP1sVnhaMt3kD78qSa7b60yiCUQGh8HZ0wqN3LaFt LVZ7/HJnC86z9fU= 16, 54 -- X-Received: by 2002:a05:6602:1da:: with SMTP id w26mr9302817iot.191.1586624170591; 16, 54 -- Sat, 11 Apr 2020 09:56:10 -0700 (PDT) 16, 54 -- MIME-Version: 1.0 16, 54 -- From: Nick Seaton {seato008-at-umn.edu} 16, 54 -- Date: Sat, 11 Apr 2020 11:55:59 -0500 16, 54 -- Message-ID: {CAMJSFzKnWoBhQP5oft0XZppEOaqR2Jq48SFmSQMZwR2TdSMQKg-at-mail.gmail.com} 16, 54 -- Subject: SEM images of microbes 16, 54 -- To: Microscopy-at-microscopy.com 16, 54 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
The big thing looks like an Oribatid mite. The setation (pattern and morphology of the hairs) would probably ID at least the family, but I don't have the necessary keys. The other 2 images ... hm. The "wormy things" could be bacterial. They might be part of the mite. Some mites do have some pretty intricate decorations. I don't think the "worms", are a coating artifact - I've never seen an artifact like this, certainly. The smaller rosettes might be coating artifact, but I don't think they are either, the shapes are too intricate and consistent.
What were the coating parameters? Time, pressure, mA?
Phil ----------------------------------------- Philip Oshel Imaging Center Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 office (989) 774-7567 lab
________________________________________ X-from: seato008-at-umn.edu {seato008-at-umn.edu} Sent: Saturday, April 11, 2020 13:01 To: Oshel, Philip Eugene
Hi folks,
I have a friend who is trying to identify the features in some SEM images but they are bio and I am hard materials. The images are at the following link https://drive.google.com/drive/folders/1vzKbmfoDZT6f9fsJduTgxx_hwKj1-sGE?usp=sharing They were collected from a white microbial mat in a cave at Lava Beds National Monument with NPS permission. They were mounted to SEM stubs with carbon tape and dried in a vacuum oven. Then coated in gold/palladium and put in the SEM. They think the big, 100 micron, thing is a mite? Any other idea welcome. What they really need to know is what are the little wormy things that look like chains of balls |--500nm in diameter? They think they might be bacteria eating the dead mite?
Nick
--
Dr Nicholas Seaton
SEM, FIB & LM Specialist Department Safety Officer Characterization Facility
College of Science and Engineering
University of Minnesota
12 Shepherd Labs
100 Union Street SE
Minneapolis
MN, 55455
email: seato008-at-umn.edu
phone: 612-626-5314
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Email: joern1911-at-gmail.com
Name: Joern
Organization: service Sem
Title-Subject: [Filtered] Leo S430 EO Board
Message: Hello Anybody,
please can you Help Please?
I have a Leo S430 SEM.
On my EO Board is a Error on C1 Coil . i have 3 ampere on the Output.
On my EO-Board is the Vision with 2 OP . I have found a Schcematic with 1 Op for the C1 Output. I think , the first Op is for the Setpoint and the second Op is for actual value from the Output currend from the C1 Coil Lens.
Have a anybody a schcematic from the EO_Board.
Thanks and best regards
Joern
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We know some institutions are shutting down completely during this pandemic. Our facility is still open (we have been designated as essential and will be required to stay open if at all possible). If you can't access your usual EM facilities, we would be glad to help. We will provide you with a D.O.T. approved shipping container kit for you to use to send your samples to us. If you need help contact me.
Tom Bargar Electron Microscopy Specialist UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 tbargar-at-unmc.edu 402-559-7347
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Email: pmccurdy-at-colostate.edu Name: Pat McCurdy
Organization: Colorado State University
Title-Subject: [Filtered] Best carbon coating method
Message: To Whom It May Concern:
We are seeking to purchase a new carbon coater. Our center does EDS and EBSD. We would like to coat down to 5 nm of carbon. We are looking at both carbon rod coaters as well as a carbon thread coater. I would appreciate your input on these two types of coaters or any additional kinds that you may have any experience with.
Thanks, Pat McCurdy Colorado State University
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With due disclaimer that (a) I've been doing C coatings mostly for charge mitigation on FIB Circuit Edit samples, and (b) below are personal impressions and not a conclusion from any kind of comparative study:
The best (perceived as smoothest, cleanest, and most uniform) carbon coatings I've seen were produced by Gatan's PECS system, using ion sputtering. I haven't operated PECS myself, but coatings made in it for me were just perfect.
Overall impression is that good cord and rod evaporation coatings typically come from turbo-pumped systems run by an operator with enough patience to wait for a full pump-down. I have been using high-vacuum version of Safematic, and despite of my initial skepticism am very pleased with it. Automated exchange of evaporation cord is oh so convenient..
No vested interest in Gatan/AMETEK or Safematic here....
Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
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Our center does EDS and EBSD. We would like to coat } down to 5 nm of carbon. We are looking at both carbon rod coaters as well as a carbon thread coater. } I would appreciate your input on these two types of coaters or any additional kinds that you may } have any experience with. } } Thanks, } Pat McCurdy } Colorado State University } } Login Host: 71.218.168.219 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 10, 53 -- From microscopy.listserver-at-gmail.com Tue Apr 14 19:46:48 2020 } 10, 53 -- Received: from mail-il1-f169.google.com (mail-il1-f169.google.com [209.85.166.169]) } 10, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 03F0klw6012677 } 10, 53 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2020 19:46:48 -0500 } 10, 53 -- Received: by mail-il1-f169.google.com with SMTP id z12so1631523ilb.10 } 10, 53 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2020 17:48:11 -0700 (PDT) } 10, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=gmail.com; s=20161025; } 10, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 10, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 10, 53 -- bh=1ee4xznFwFyrBO+xx2iSZ7NNuqJR5XCk6VlAjPUG1c4=; } 10, 53 -- b=oLL8ass6fcT1yng++a/9cfk2yUjc0VIhk7lScAAMj/+esVR/uJAsHD5gW7BG31h388 } 10, 53 -- SL8ksAPWdvU9gvDrakZrHk4er9oT/+ZajMq5BtVsGwU86DOz8OvOeP00jHpmbRshkVJP } 10, 53 -- ae2MBYPN57IIHIWNhsVk7vaOl0T6y63/ATeYUvnQEuoQxvot+jlZBlPoNyIMPWZgrAHb } 10, 53 -- Xup23eCPu/vu29nd5usmLKr9SJyq1qj81SJtFUl+1+VnUwb0erY00IxNKDrxryEswWZm } 10, 53 -- Ts5VKUKPjBDiV2U2geN7twQX6rr2rgmjrlzRCEWShrB9E9N7+vJVRNErm3tTjLQb3s/s } 10, 53 -- Ap0w== } 10, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 53 -- d=1e100.net; s=20161025; } 10, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 10, 53 -- :user-agent:mime-version:in-reply-to:content-language } 10, 53 -- :content-transfer-encoding; } 10, 53 -- bh=1ee4xznFwFyrBO+xx2iSZ7NNuqJR5XCk6VlAjPUG1c4=; } 10, 53 -- b=AlwytNlowa3WulcJuZIHYElcwkm+ZBvQkP32kPsWWuzPKOjIyWsSkDpOMMuCAWjPqm } 10, 53 -- +v/cVJBawZX5FL4grusdWaqbuto2GsF2pBAhQyajq10wejaDQgegMHfNYzflVEP60/5a } 10, 53 -- QdYNuWwbsk5/GWdUg8bbyPZCZeUy4jGZH+VqBjZDVr+DLEx+GSPy7lnjSCXIZY/7HO+I } 10, 53 -- dj7ctICdoZuVVlxCeWNulJZGXp+pq/X3lKU7/FEqRqYheAbaf3Lwzee0jrBgoaXm/msR } 10, 53 -- Vu6aqh2zbFJO9mtZjKIfGx+2w14TQeEobaeF2APgKN9agFQgMafNy68pDfo/BLqk6ZeA } 10, 53 -- wGZw== } 10, 53 -- X-Gm-Message-State: AGi0PuYGQJrC6IKCtVEqzdzZY6Q9ZArWtEJDqhsJ8ZBi88JYFlaWHBBu } 10, 53 -- T+AovQ7AHSptNqVZjNdaYRNky0yT } 10, 53 -- X-Google-Smtp-Source: APiQypIXsIRUwMPEBl0zhyQWIowV9wKGLydWDfLuZNjuqIUqp3gaHAbDKc81aE9nQWZsbtH6ia8ZWg== } 10, 53 -- X-Received: by 2002:a92:5f17:: with SMTP id t23mr3086187ilb.2.1586911691128; } 10, 53 -- Tue, 14 Apr 2020 17:48:11 -0700 (PDT) } 10, 53 -- Received: from anlvpn001.nst.anl.gov ([130.202.235.1]) } 10, 53 -- by smtp.googlemail.com with ESMTPSA id u20sm1748594iow.7.2020.04.14.17.48.10 } 10, 53 -- for {microscopy-at-microscopy.com} } 10, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 10, 53 -- Tue, 14 Apr 2020 17:48:10 -0700 (PDT) } 10, 53 -- Subject: viaWWW: Best carbon coating method } 10, 53 -- References: {202004141748.03EHmiHT017504-at-microscopy.com} } 10, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 10, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 10, 53 -- X-Forwarded-Message-Id: {202004141748.03EHmiHT017504-at-microscopy.com} } 10, 53 -- Message-ID: {02f5abf3-62ab-a8ae-254a-401c1e89a4ad-at-gmail.com} } 10, 53 -- Date: Tue, 14 Apr 2020 19:48:09 -0500 } 10, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 10, 53 -- Gecko/20100101 Thunderbird/68.7.0 } 10, 53 -- MIME-Version: 1.0 } 10, 53 -- In-Reply-To: {202004141748.03EHmiHT017504-at-microscopy.com} } 10, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 10, 53 -- Content-Language: en-US } 10, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
From andralbi54c-at-gmail.com Wed Apr 15 13:13:36 2020 Return-Path: {andralbi54c-at-gmail.com} Received: from gmail.com (karibu.dreformarra.com [134.73.232.206]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 03FIDY38022005 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 15 Apr 2020 13:13:34 -0500 Message-ID: {CF822039.D410E819-at-gmail.com}
X-from: rvancamp-at-kettering.edu
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Message: Please understand I am as surprised to post this issue as you are at reading it. This adjustment is usually performed without incident. However, in reading notes from previous alignment efforts with our JEOL 2100+ I find/recall that there was a period of time when I found removing the oval shape of the beam at 40k MAG virtually impossible. This occurred during the latter stages of our W-filament life. This may be a strong contributing factor. I am hoping some of you can improve my understanding and problem solving skills associated with this phenomena.
Use of the 2d function pendant controls did not do the trick as they typically do. As I continued to think about how to remove the CL astigmatism I first reduced the TEM to 20k MAG and experienced excellent results on several occasions. However, as I continued to think about this issue I suspected I needed to migrate further up the column. This implied I needed to examine the Gun Alignment. I find some additional improvement here with adjustments to gun tilt. Despite this, I would openly state the effort at 20k MAG was a greater gain than the work gun tilt.
Please try to help me understand this concern better and shoot me down as is necessary....I've been in physical science a long time...I am used to this arcane treatment! If I did something wrong I need to know. It is that simple.
Also note I am not well skilled with the "Filament Image" function on our microscope but I currently suspect I have the right understanding regarding the use of this control. Use it at cross over. I have observed the filament image function used on an SEM before but this was a completely different image/result so I see no meaningful comparison at this time. I have frequently used the symmetric structure of Cat's Eye when aligning a W-filament but this has been during the transient conditions during Beam On/Off. I recorded these observations using my smartphone camera to refer to them as a learning aide. I obtained 272 h from my initial attempt aligning a K-type filament so I am pleased with this result but I have a lot to learn.
Thank you for your time.
Rick
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You were probably seeing coma, rather than astigmatism, caused by a badly aligned beam tilt, mistakenly corrected with beam shift. They can look very similar, but if it is tilt when you hit the HT wobbler button you will see the beam move across the screen and change shape. Adjust BF tilt until the wobbling is concentric (you will probably need to adjust beam shift too to keep the beam on the screen).
Having said this, if you are trying to align alpha 1 rather than alpha 3 the rules are different. If this is the case let me know and I can give you the more complicated version! [In the U.K. we are locked down and this is the closest I am going to get to touching a TEM for the next month I think. Stay safe.]
Best wishes Richard
} On 17 Apr 2020, at 16:32, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: rvancamp-at-kettering.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } rvancamp-at-kettering.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers } can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: rvancamp-at-kettering.edu } } Name: Rick Van Camp } } Organization: Kettering University } } Title-Subject: [Filtered] Correcting CL Astigmatism } } Message: Please understand I am as surprised to post this issue as you are at reading it. This } adjustment is usually performed without incident. However, in reading notes from previous alignment } efforts with our JEOL 2100+ I find/recall that there was a period of time when I found removing the } oval shape of the beam at 40k MAG virtually impossible. This occurred during the latter stages of } our W-filament life. This may be a strong contributing factor. I am hoping some of you can improve } my understanding and problem solving skills associated with this phenomena. } } Use of the 2d function pendant controls did not do the trick as they typically do. As I continued } to think about how to remove the CL astigmatism I first reduced the TEM to 20k MAG and experienced } excellent results on several occasions. However, as I continued to think about this issue I } suspected I needed to migrate further up the column. This implied I needed to examine the Gun } Alignment. I find some additional improvement here with adjustments to gun tilt. Despite this, I } would openly state the effort at 20k MAG was a greater gain than the work gun tilt. } } Please try to help me understand this concern better and shoot me down as is necessary....I've been } in physical science a long time...I am used to this arcane treatment! If I did something wrong I } need to know. It is that simple. } } Also note I am not well skilled with the "Filament Image" function on our microscope but I currently } suspect I have the right understanding regarding the use of this control. Use it at cross over. I } have observed the filament image function used on an SEM before but this was a completely different } image/result so I see no meaningful comparison at this time. I have frequently used the symmetric } structure of Cat's Eye when aligning a W-filament but this has been during the transient conditions } during Beam On/Off. I recorded these observations using my smartphone camera to refer to them as a } learning aide. I obtained 272 h from my initial attempt aligning a K-type filament so I am pleased } with this result but I have a lot to learn. } } Thank you for your time. } } Rick } } Login Host: 68.40.45.131 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 14, 53 -- From microscopy.listserver-at-gmail.com Fri Apr 17 10:31:47 2020 } 14, 53 -- Received: from mail-il1-f177.google.com (mail-il1-f177.google.com [209.85.166.177]) } 14, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 03HFVlc5000438 } 14, 53 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2020 10:31:47 -0500 } 14, 53 -- Received: by mail-il1-f177.google.com with SMTP id s10so1749224iln.11 } 14, 53 -- for {microscopy-at-microscopy.com} ; Fri, 17 Apr 2020 08:33:20 -0700 (PDT) } 14, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 14, 53 -- d=gmail.com; s=20161025; } 14, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 14, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 14, 53 -- bh=GqtEkmGCKWehLf8eHrWLpv6fKcv/Zw0pwRQqPlUPMQw=; } 14, 53 -- b=K1s6qaPJJ+hh498POMgb3LgzzEnew4xV1jt72nwLprds9cPRacuA32+p0nmS3n3GJz } 14, 53 -- uZfuFxb8JBDahf+EzD9J85kOiDXHA4L9C182WIwZHbbF11RTM6Z4eu2HcdmBb7oYN0wB } 14, 53 -- GyKlxuLPweMQHlJ1ZKRWkGpcqrrTT8rNhJS5E1IH3Jlr9Dzkr3iX+TgiQqVJp1G8xP8/ } 14, 53 -- C+YxEp1wPjdrq/NC5cN+R3zriSHdsWKRrb9QOcio6GsZgmpqTqyrgKEdPQoW5jgWj3CI } 14, 53 -- yfNk/9jMEccRa39fCEcoi2bRnoFV617DVpcvqHk/4S5emzjcbTVOqo5ra8WdZ1aPsT7o } 14, 53 -- 41jA== } 14, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 14, 53 -- d=1e100.net; s=20161025; } 14, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 14, 53 -- :user-agent:mime-version:in-reply-to:content-language } 14, 53 -- :content-transfer-encoding; } 14, 53 -- bh=GqtEkmGCKWehLf8eHrWLpv6fKcv/Zw0pwRQqPlUPMQw=; } 14, 53 -- b=akLT0LAF0XYISaoqSwWOVlnKFpDo9n5chxSxQ/YqPYcBmjcrVlGFFH34QZoshePjKu } 14, 53 -- FoYYwjntPk2s1uswFfZkoTn/tzNmDmYBxtz5kBIfn8qUqA51kO6N+t/CRK1vAOlAa/sc } 14, 53 -- vzhCmlIuUKhiic9IWTErWEzOO5jCub1KGwHpTXR8KpDhOdWm+fkcr7dHL4ZhNEzHGLOj } 14, 53 -- JX5BxSjFtx3ytYz4XncXZUqS5h9/KpbbF3vR4gkS/ZaXVTinPWiaqlSl2j7MXbTe7EIL } 14, 53 -- 7HmUCv9ujlsSSQE5GumRDRbIodMwSwaqinJ44MJqPNFfuxebcDF0VCt04UoRJhVOZV1K } 14, 53 -- M6+A== } 14, 53 -- X-Gm-Message-State: AGi0PuY0Dc0w2Ese1PAUwxBJNbqOEDG1lEDeNex7w2khADAvO7jnnCOB } 14, 53 -- v4517ZjTmNt21RFjq5DQZSAu8St7 } 14, 53 -- X-Google-Smtp-Source: APiQypLTu3ptqIzFALHkk+u8wqYDQ/r4sfB6t9qY3kjwhPvN/qZbQO+kCnm8f9n6k8lomuEbUJ1nlg== } 14, 53 -- X-Received: by 2002:a92:d44b:: with SMTP id r11mr3511209ilm.120.1587137599419; } 14, 53 -- Fri, 17 Apr 2020 08:33:19 -0700 (PDT) } 14, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:85ca:a902:98f2:1e67]) } 14, 53 -- by smtp.googlemail.com with ESMTPSA id z21sm4939290iog.31.2020.04.17.08.33.18 } 14, 53 -- for {microscopy-at-microscopy.com} } 14, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 14, 53 -- Fri, 17 Apr 2020 08:33:18 -0700 (PDT) } 14, 53 -- Subject: viaWWW: Correcting CL Astigmatism } 14, 53 -- References: {202004171516.03HFGaCD032177-at-microscopy.com} } 14, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 14, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 14, 53 -- X-Forwarded-Message-Id: {202004171516.03HFGaCD032177-at-microscopy.com} } 14, 53 -- Message-ID: {3020eff6-7640-8dc4-399d-2a1e1ba6e6af-at-gmail.com} } 14, 53 -- Date: Fri, 17 Apr 2020 10:33:17 -0500 } 14, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 14, 53 -- Gecko/20100101 Thunderbird/68.7.0 } 14, 53 -- MIME-Version: 1.0 } 14, 53 -- In-Reply-To: {202004171516.03HFGaCD032177-at-microscopy.com} } 14, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 14, 53 -- Content-Language: en-US } 14, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
I have only done carbon rod and an old Gatan PECS ion deposition system. My qualitative observations are below and I have no financial interest in coating materials companies.
The PECS is slow, expensive to operate, and for our samples was limited to one at a time, but it worked well until we stopped maintaining it due to lack of use.
We have a 10 year old EMS rod type turbo pumped carbon coater (not the one they currently sell). It works fine down to 3nm on EBSD, but is a bit trickier to get the desired thickness. Consistently sharpening the rod is key to getting a consistent thickness. Some of my geology people complain that they have to run two coating cycles since the sharpened rod will not get them to 25nm if they are going to microprobe.
If you want a high quality C coat high vacuum is a must.
Greg
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Email: pmccurdy-at-colostate.edu {mailto:pmccurdy-at-colostate.edu} Name: Pat McCurdy
Organization: Colorado State University
Title-Subject: [Filtered] Best carbon coating method
Message: To Whom It May Concern:
We are seeking to purchase a new carbon coater. Our center does EDS and EBSD. We would like to coat down to 5 nm of carbon. We are looking at both carbon rod coaters as well as a carbon thread coater. I would appreciate your input on these two types of coaters or any additional kinds that you may have any experience with.
Thanks, Pat McCurdy Colorado State University
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With due disclaimer that (a) I've been doing C coatings mostly for charge mitigation on FIB Circuit Edit samples, and (b) below are personal impressions and not a conclusion from any kind of comparative study:
The best (perceived as smoothest, cleanest, and most uniform) carbon coatings I've seen were produced by Gatan's PECS system, using ion sputtering. I haven't operated PECS myself, but coatings made in it for me were just perfect.
Overall impression is that good cord and rod evaporation coatings typically come from turbo-pumped systems run by an operator with enough patience to wait for a full pump-down. I have been using high-vacuum version of Safematic, and despite of my initial skepticism am very pleased with it. Automated exchange of evaporation cord is oh so convenient..
No vested interest in Gatan/AMETEK or Safematic here....
Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 4/14/2020 8:47 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: pmccurdy-at-colostate.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } pmccurdy-at-colostate.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers } can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: pmccurdy-at-colostate.edu Name: Pat McCurdy } } Organization: Colorado State University } } Title-Subject: [Filtered] Best carbon coating method } } Message: To Whom It May Concern: } } We are seeking to purchase a new carbon coater. Our center does EDS and EBSD. We would like to coat } down to 5 nm of carbon. We are looking at both carbon rod coaters as well as a carbon thread coater. } I would appreciate your input on these two types of coaters or any additional kinds that you may } have any experience with. } } Thanks, } Pat McCurdy } Colorado State University } } Login Host: 71.218.168.219 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 10, 53 -- From microscopy.listserver-at-gmail.com Tue Apr 14 19:46:48 2020 } 10, 53 -- Received: from mail-il1-f169.google.com (mail-il1-f169.google.com [209.85.166.169]) } 10, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 03F0klw6012677 } 10, 53 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2020 19:46:48 -0500 } 10, 53 -- Received: by mail-il1-f169.google.com with SMTP id z12so1631523ilb.10 } 10, 53 -- for {microscopy-at-microscopy.com} ; Tue, 14 Apr 2020 17:48:11 -0700 (PDT) } 10, 53 -- DKIM-Signature: v=1; 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Patrick - great question for the ListServ. There are advantages for both Rod and Thread for Carbon Coating. There is also e-Beam Carbon and Thermal Evaporation which are more spendy but can control a more precise and thinner layer.
If you go with traditional rod or thread coating, then you should get a system with high vacuum (TMP) to have a finer grain size since you want a layer down to 5nm. While a carbon rod can be more precise for very thin layers and finer grain size, some thread systems can be pulsed so the coating occurs slower to produce good control of thin layers also. Thread systems are also a little easier to use since you don not have to sharpen and handle delicate carbon rods. A thread system would be good for new users that just want a quick conductive coating.
Why compromise though? There are a couple systems that can do both Rod or Thread or can add Rod capability at a later date as an upgrade. We offer such a coater, see this link: https://elementpi.com/sputter-coaters-carbon-evaporators/
Good luck with your research!
Mike Toalson
element Pi 833.314.1593 mike.toalson-at-elementpi.com {mailto:mike.toalson-at-elementpi.com} web www.elementpi.com {http://www.elementpi.com}
We have a Denton 502A Carbon Coater with a turbo molecular pump and Cressington thickness monitor. Using carbon rods we deposit between 7-10 nm for CL and 20 nm for imaging, EDS, and EPMA. It has a cold finger so liquid nitrogen can be added to hasten a quick coat, otherwise an hour pump down gets us to 10 to the minus 6 torr. The stage rotates as its a line of site coater, 10 one inch rounds or maybe 5 thin sections can go in at one pump down.
We do a ton of EBSD, but rely on a Leica Ace600 coater to apply a 1nm coat of Iridium to any EBSD sample. It is flawless on geological thin sections and never charges.
Bil Schneider UW Madison Geosciences SEM Lab Manager wfschneider-at-wisc.edu
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Email: pmccurdy-at-colostate.edu Name: Pat McCurdy
Organization: Colorado State University
Title-Subject: [Filtered] Best carbon coating method
Message: To Whom It May Concern:
We are seeking to purchase a new carbon coater. Our center does EDS and EBSD. We would like to coat down to 5 nm of carbon. We are looking at both carbon rod coaters as well as a carbon thread coater. I would appreciate your input on these two types of coaters or any additional kinds that you may have any experience with.
Thanks, Pat McCurdy Colorado State University
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Hello dear colleagues!
Specially to my american fellows, I want to offer my compassion and sympathy in the hard times you (we) are currently experiencing. I have a specific question regarding the preparation of biological material containing minerals for FIB-SEM (look at it like for example the examination of wound skin biopsies upon application of submicroparticles). The purpose is to etch a block parallel to the surface and sequentially using a FIB-SEM (like horizonzal sectionning but with a FIB) and looking for mineral particles inside the block, building a 3D image of the block. We performed a preliminary experiment showing that the particles can be easily detected (particle contrast and resolution are good enough). However, we faced 2 problems: (1) paraffin is etched much too fast compared to the particles inside the sample (2) we got a strong charging effect
So now I am looking for alternative embedding material which could replace paraffin. Does anyone have any experiences with epoxy blocks in FIB-SEM? Can I expect epoxy resins to be harder to etch than paraffin? Is there a rule to a priori calculate the "hardness" (FIB-etchiness) of a material to be etched to match the embedding material to the particles embedded inside? How can I make an epoxy resin conductive? I thought above mixing silver nanoparticles in the embedding material (they wouldn't disturb the analysis because they are very different from the particles we are looking for). Does anyone have an experience to share? Thank you in advance and stay safe!
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I thought I understood how phase contrast microscopy works, but then I was reading MicroscopyU (https://www.microscopyu.com/techniques/phase-contrast/introduction-to-phase-contrast-microscopy) and other sites and now I am confused. My understanding is that it works because of refractive index differences in different parts of the light path (cells) leading to retardation of refracted light and eventual phase differences relative to un-refracted (surround) light that passes through the thinned area of the phase plate in the objective. However, in the web site indicated and others, they use refracted light and diffracted light almost interchangeably in explaining phase contrast. To my “biologist” level understanding, diffraction and refraction are very different phenomenon and I did not think that diffraction changed the optical path length like refractive index differences. Refraction makes total sense to me in the context of phase contrast, but I don’t see how diffraction is relevant. Can someone explain what I am missing? Thanks, Dave
Dr. David Knecht Professor, Department of Molecular and Cell Biology University of Connecticut 91 N. Eagleville Rd. U-3125 Storrs, CT 06269-3125
I am a biologist too and sometimes have a hard time to understand such complex physical phenomena, so perhaps I can use the right words for you to understand.
My understanding is: - Diffraction is due to the interaction of the focused incoming light on the sample. Diffraction is the reason why an object is visible in light and electron microscopy. - Refraction is related to the change in light speed through a change in the medium (lense) thickness, here the phase ring (which you must choose according to the objective used).
The principle here is to separate in physical space the normal (not diffracted) light from the light diffracted by the sample. If you look at the picture shown in the link you gave, you see only a yellow beam before the sample but after the sample you see a light pink color between (and also outside) the yellow beams (called direct surround light). This is the diffracted light (the lines point to the rosy color between the yellow light beams).
I think I identified your problem : the phase difference doesn't occur by passing through the sample, it occurs due to the phase ring placed after (or within) the objective lense. The interaction of light with the sample give you diffracted, not refracted light (or perhaps some negligible refraction).
The diffracted light (by the specimen) will be refracted by the objective lense just as the non-diffracted light is BUT it will not strike the lense at the same place. Only the non-diffracted light with pass through the phase ring (because the ring is calculated to be in the path of non-diffracted light only) and this will result in a phase difference between the light diffracted by the specimen and the light that passes through it unchanged. This difference is then reconstructed on the image plane to give a contrast.
I hope I could help, it is hard to explain a 3D view only with words.
Stay safe and don't hesitate to ask for more explanations
Stephane
On Wednesday, April 22, 2020, 03:48:03 PM GMT+2, david.knecht-at-uconn.edu {david.knecht-at-uconn.edu} wrote:
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I thought I understood how phase contrast microscopy works, but then I was reading MicroscopyU (https://www.microscopyu.com/techniques/phase-contrast/introduction-to-phase-contrast-microscopy) and other sites and now I am confused. My understanding is that it works because of refractive index differences in different parts of the light path (cells) leading to retardation of refracted light and eventual phase differences relative to un-refracted (surround) light that passes through the thinned area of the phase plate in the objective. However, in the web site indicated and others, they use refracted light and diffracted light almost interchangeably in explaining phase contrast. To my “biologist” level understanding, diffraction and refraction are very different phenomenon and I did not think that diffraction changed the optical path length like refractive index differences. Refraction makes total sense to me in the context of phase contrast, but I don’t see how diff! raction is relevant. Can someone explain what I am missing? Thanks, Dave
Dr. David Knecht Professor, Department of Molecular and Cell Biology University of Connecticut 91 N. Eagleville Rd. U-3125 Storrs, CT 06269-3125
With due disclaimer that I am doing ion and electron beam processing and my recollection of light optics is from loooong time ago - diffraction encompasses multitude of phenomena, including separating spectral components of white light and possible interference between waves diffracted at different locations of the object. Separation of the spectrum with white light illumination and interference could both produce delayed wavefront resulting in phase contrast.
My feeling however is that most of phase contrast from thin, uniform, transparent biological specimens would be produced by dirrerences in the index of refraction...
"Diffracted light" notation on microscopyu page refers to separation of diffraction orders within the light path of the microscope. During diffraction contrast imaging direct light (DC background) is blocked by illumination aperture (condenser annulus) and the diffraction plate, while first-order diffracted light is passed to image plane to form phase-contrast images.
I'm sure that many people with more current and fundamental knowledge of optical microscopy will correct and expand my qualitative and exceedingly hand-waving explanation...
Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 4/22/2020 9:40 AM, david.knecht-at-uconn.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I thought I understood how phase contrast microscopy works, but then I was reading MicroscopyU (https://www.microscopyu.com/techniques/phase-contrast/introduction-to-phase-contrast-microscopy) and other sites and now I am confused. My understanding is that it works because of refractive index differences in different parts of the light path (cells) leading to retardation of refracted light and eventual phase differences relative to un-refracted (surround) light that passes through the thinned area of the phase plate in the objective. However, in the web site indicated and others, they use refracted light and diffracted light almost interchangeably in explaining phase contrast. To my “biologist†level understanding, diffraction and refraction are very different phenomenon and I did not think that diffraction changed the optical path length like refractive index differences. Refraction makes total sense to me in the context of phase contrast, but I don’t see how diff! } raction is relevant. Can someone explain what I am missing? Thanks, Dave } } Dr. David Knecht } Professor, Department of Molecular and Cell Biology } University of Connecticut } 91 N. Eagleville Rd. } U-3125 } Storrs, CT 06269-3125 } } } } } } ==============================Original Headers============================== } 6, 74 -- From david.knecht-at-uconn.edu Wed Apr 22 08:39:52 2020 } 6, 74 -- Received: from NAM12-MW2-obe.outbound.protection.outlook.com (mail-mw2nam12on2100.outbound.protection.outlook.com [40.107.244.100]) } 6, 74 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 03MDdpU5003878 } 6, 74 -- for {Microscopy-at-Microscopy.Com} ; Wed, 22 Apr 2020 08:39:51 -0500 } 6, 74 -- ARC-Seal: i=1; a=rsa-sha256; s=arcselector9901; d=microsoft.com; cv=none; } 6, 74 -- b=eYDdp2RGuh8RDvuaTaa/Z+igU01ijJcW7MbL76yzwO3QZEc69UnmJsiCIrsPMZngA3MW/e95RGDfSKnvjihhsVak0q8yCZG47HfMl/ujkrHNSKckQmx0WQaDEhcAhH+RwGx6DhZv+HuxJwXDdhucAJxb1nwThkuhn5P2ik180oCNHXM77CRe7n8OVDSxLIPRMxQ7LmO446wnd3YOwP5QgLafbITruyBVjMvOG3qdHXitlLMmh7AjfK6YMuxCFH3vsD1uXjhFHmuGeJG8ftunG5GWl5SEzwT8yGwg6gijk62mseqzuTYQwM1s+y1WomligyB6i6SpbB9pIMx4CfzH2g== } 6, 74 -- ARC-Message-Signature: i=1; a=rsa-sha256; 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With due disclaimer that I am doing ion and electron beam processing and my recollection of light optics is from loooong time ago - diffraction encompasses multitude of phenomena, including separating spectral components of white light and possible interference between waves diffracted at different locations of the object. Separation of the spectrum with white light illumination and interference could both produce delayed wavefront resulting in phase contrast.
My feeling however is that most of phase contrast from thin, uniform, transparent biological specimens would be produced by dirrerences in the index of refraction...
"Diffracted light" notation on microscopyu page refers to separation of diffraction orders within the light path of the microscope. During diffraction contrast imaging direct light (DC background) is blocked by illumination aperture (condenser annulus) and the diffraction plate, while first-order diffracted light is passed to image plane to form phase-contrast images.
I'm sure that many people with more current and fundamental knowledge of optical microscopy will correct and expand my qualitative and exceedingly hand-waving explanation...
Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 www.partbeamsystech.com www.freudlabs.com E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479
On 4/22/2020 9:40 AM, david.knecht-at-uconn.edu wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I thought I understood how phase contrast microscopy works, but then I was reading MicroscopyU (https://www.microscopyu.com/techniques/phase-contrast/introduction-to-phase-contrast-microscopy) and other sites and now I am confused. My understanding is that it works because of refractive index differences in different parts of the light path (cells) leading to retardation of refracted light and eventual phase differences relative to un-refracted (surround) light that passes through the thinned area of the phase plate in the objective. However, in the web site indicated and others, they use refracted light and diffracted light almost interchangeably in explaining phase contrast. To my “biologist†level understanding, diffraction and refraction are very different phenomenon and I did not think that diffraction changed the optical path length like refractive index differences. Refraction makes total sense to me in the context of phase contrast, but I don’t see how diff! } raction is relevant. Can someone explain what I am missing? Thanks, Dave } } Dr. David Knecht } Professor, Department of Molecular and Cell Biology } University of Connecticut } 91 N. 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As one of the symposium organizers for EMC 2020 23-28 August in Copenhagen I have just received the announcement that the meeting has been canceled as per the announcement from the Danish Government. The link to the official announcement prohibiting meetings of more than 500 people until after Sept 1 is below.
I also have receive confirming Email from the Royal Microscopy Society of the cancellation.
The "rough translation" of the Danish announcement on the above WWW site is:
--------------------------------------------------------------------------------------------------------- PRESS RELEASE - The government has decided to set an upper limit for assemblies of 500 people. It will be valid until 1 September 2020.
The government has decided that a ban on major events will be prohibited over the summer and until 1 September 2020. The limit for large events has now been set so that events with more than 500 people may not be held. The decision is made as a result of the health authorities' assessment that the corona epidemic will continue to develop in Denmark over the coming months. However, setting the ceiling of 500 people for large events will not have any practical significance in the first place.
The ban on gathering more than 10 people was extended on Friday up to and including 10 May 2020.
The government closely monitors the development of the epidemic and therefore also adjusts the limit on how many people are allowed to congregate on an ongoing basis.
Contact Info: Ministry of Health and Senior Services, Press Telephone: 21 32 47 27 ---------------------------------------------------------------------------------------------------------
From koepketemi17uno-at-gmail.com Thu Apr 23 04:31:23 2020 Return-Path: {koepketemi17uno-at-gmail.com} Received: from gmail.com (wpcnn.dreformarra.com [134.73.232.208]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 03N9VMkY009972 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 23 Apr 2020 04:31:22 -0500 Message-ID: {357C82DD.EC9F911A-at-gmail.com}
X-from: smodla-at-udel.edu
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Email: smodla-at-udel.edu Name: Shannon Modla
Organization: University of Delaware
Title-Subject: [Filtered] Third Party Service for TEM
Message: The manufacturer of our TEM will be ending service within two years. It's a 120kV LaB6 TEM running on Windows XP. Does anyone have advice or can recommend another company that would provide service to the instrument until we are able to acquire funds for a replacement?
Thanks,
Shannon Research Associate III University of Delaware
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Email: john.hyun-at-ametek.com
Name: John Hyun
Organization: Gatan
Title-Subject: [Filtered] Virtual M&M 2020 or emc 2020?
Message: We hope you are safe and well. In lieu of a live conference, would you be interested in a virtual 2020 M&M Meeting or virtual emc 2020? M&M is still scheduled as a live event. These are our industry's premiere events, and it would be a shame to cancel one or both without a real option to "attend." Fantastic virtual platforms and management companies are available. This will be the future of events.
If you would like to see M&M and emc go virtual, we encourage you to email the meeting managers (see below) with your support. Lets meet in a safe and dynamic way!
If it is a JEOL, try Joe Bricker. I'm sure some ex-engineers from other companies can handle those EMs. Start by asking your regular service engineer whom he might know.
On 4/29/2020 11:37 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: smodla-at-udel.edu } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.msa.microscopy.org/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } smodla-at-udel.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: smodla-at-udel.edu Name: Shannon Modla } } Organization: University of Delaware } } Title-Subject: [Filtered] Third Party Service for TEM } } Message: The manufacturer of our TEM will be ending service within two years. It's a 120kV LaB6 TEM } running on Windows XP. Does anyone have advice or can recommend another company that would provide } service to the instrument until we are able to acquire funds for a replacement? } } Thanks, } } Shannon } Research Associate III } University of Delaware } } Login Host: 76.99.200.236 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 11, 53 -- From microscopy.listserver-at-gmail.com Wed Apr 29 10:33:24 2020 } 11, 53 -- Received: from mail-il1-f181.google.com (mail-il1-f181.google.com [209.85.166.181]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 03TFXO5I008813 } 11, 53 -- for {microscopy-at-microscopy.com} ; Wed, 29 Apr 2020 10:33:24 -0500 } 11, 53 -- Received: by mail-il1-f181.google.com with SMTP id i16so2792807ils.12 } 11, 53 -- for {microscopy-at-microscopy.com} ; Wed, 29 Apr 2020 08:35:35 -0700 (PDT) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=YLGbziFVo+uofsuGhTKV3nA2ClpvWNnD0SzDm/932Bs=; } 11, 53 -- b=gNxHfsgPA6sdZiNSRyleDf3Ul1Caf7bbvPhSSoLHuZyYvT0sLA3abbA3e3PO/qkQnB } 11, 53 -- RZNnFZKIyfA6Qz0bjKe8PLHJYiekczO660uKTdTg2khIezlbta+PC1lRy33H0aCWvHYS } 11, 53 -- sn1zy3X1+acih60+YxLTxHZx1r+9CSEs5N6mtoS2QCNFVte6VvgfUtHj0NgPPKOZLYug } 11, 53 -- v5mNR88OhnADHlyx+ZntqH3JRriJnuupCKF+Gj0zaJszU2kyPnrBnORdHNmzBVJIoBLK } 11, 53 -- nHFiW33+LrEzT8S6g9DstWdTMILLDhA52wSJ/2nlUdcopV902Bvpb+wuMhQjccE8S62U } 11, 53 -- Yo8g== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=YLGbziFVo+uofsuGhTKV3nA2ClpvWNnD0SzDm/932Bs=; } 11, 53 -- b=sQu+EpLSoGuP0inf9GAPL3rCdIK1SU/1TzaRatvw8JE6mQS576i5K0QZvLeUQwin// } 11, 53 -- ceqvRJhLpj0C+8zmQOELlHA9k+CPRqTYs7RJJ8Wm9m4iRtG1MTcQ8yeYHvd3FKsAA+/u } 11, 53 -- E7DMTVcGrwnObICpQQJWzRCIH3CaeczuteflohZuYpOC8GlNjy9oE6vC5cQsNZuPcnep } 11, 53 -- p5AB6BFbmjnkXgXsIyguFSIWHSEDPOAnraKbkd5XPf11UpEzWKYX0qZ511/CXGA7qn5V } 11, 53 -- ZSBVnTXY8p68thnkvGNJX1B4z5dMOFWa5zLZu4HfvEfTkU/J6x8VFzM3/HGkuIE9JpOi } 11, 53 -- PCmQ== } 11, 53 -- X-Gm-Message-State: AGi0PubH5BgCKMVzFuhNyknu7LlRj9/T5M+8xUxUAME65TrfJMVIW//9 } 11, 53 -- ZgW4BzxwpTB31lJSg4jzaEJBxN/8 } 11, 53 -- X-Google-Smtp-Source: APiQypJnDFxzknfvI+kf1nstVqjaZ5u+1EP/o6ZYj1MYXmcXrUaLALcljh/3/O6FEA6XJKXHsgZCFg== } 11, 53 -- X-Received: by 2002:a92:d912:: with SMTP id s18mr29050585iln.30.1588174535155; } 11, 53 -- Wed, 29 Apr 2020 08:35:35 -0700 (PDT) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:697c:3b96:960e:6784]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id y3sm1089561ilp.54.2020.04.29.08.35.34 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 11, 53 -- Wed, 29 Apr 2020 08:35:34 -0700 (PDT) } 11, 53 -- Subject: viaWWW:Third Party Service for TEM } 11, 53 -- References: {202004291430.03TEUdsc005063-at-microscopy.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: {202004291430.03TEUdsc005063-at-microscopy.com} } 11, 53 -- Message-ID: {e1ae30c5-ce61-14d1-44aa-53c3e30b3e15-at-gmail.com} } 11, 53 -- Date: Wed, 29 Apr 2020 10:35:33 -0500 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 11, 53 -- Gecko/20100101 Thunderbird/68.7.0 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: {202004291430.03TEUdsc005063-at-microscopy.com} } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
From gunnclar77z-at-gmail.com Fri May 1 16:30:21 2020 Return-Path: {gunnclar77z-at-gmail.com} Received: from gmail.com (asovou.whillegain.com [134.73.232.199]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 041LULmR008612 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 1 May 2020 16:30:21 -0500 Message-ID: {959067AB.1930B568-at-gmail.com}
X-from: Raffaella.carzaniga-at-crick.ac.uk
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Email: Raffaella.carzaniga-at-crick.ac.uk
Name: Raffa Carzaniga
Organization: The Francis Crick Institute
Title-Subject: [Filtered] Webinar: Imaging SARS-CoV-2 safely: Protecting the microscopy community (Friday 22nd May 13:00-16:00)
Message: Dear Colleagues, A webminar about Imaging SARS-CoV-2 safely: Protecting the microscopy community, which will take place virtually on Friday 22nd May from 13:00 to 16:00 BST. Please find further details below, and visit the following link to register:
Meeting description: As the SARS-CoV-2 pandemic progresses, scientific focus will gradually shift from frontline testing and tracking capability, to studying the fundamental cell biology of the virus and clinical progression of virus infection in human tissues. Imaging virus-infected cells and tissues with light, electron and X-ray microscopes will play a critical role in understanding the pathogen and in designing future vaccines and therapies. In this context, it is likely that research and diagnostic imaging facilities will soon face requests from researchers and clinicians to handle virus-infected samples. It is therefore critical that we understand how to safely handle these samples, and ensure that we protect the UK microscopy community from lab-acquired infections. This webinar brings together expert pathologists, microscopists and virologists who are studying inactivation of virus in cells and tissues for safe handling, imaging the cell biology of viruses, and handling virus-infected human tissues. Invited speakers will give short talks sharing their expertise, followed by a Q&A session to answer questions from the microscopy community. Programme 13:00 - 13:10 Introduction and webinar etiquette Lucy Collinson (Francis Crick Institute, London, UK) 13:10 - 13:30 Looking CoViD-19 in the eye: a perspective from an NHS histopathologist Dr Emyr Wyn Benbow (University of Manchester/Manchester Royal Infirmary) 13:30 - 13:50 Virus Inactivation for Imaging how to get images of SARS-CoV-2 without endangering yourself or the community Dr Matthew Hannah (Public Health England) 13:50 - 14:10 Imaging viruses in high containment research labs Professor Pippa Hawes (The Pirbright Institute) 14:10 - 14:30 Understanding coronavirus replication organelles Dr Helena Maier (The Pirbright Institute) 14:30 - 14:50 The virologists toolbox. An overview of methods to manipulate RNA viruses and cells for imaging Dr Rachel Ulferts (Francis Crick Institute) 14:50 - 16:00 Panel Discussion/ Q&A All
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From johnsonloretta061voaz-at-gmail.com Thu May 21 16:19:56 2020 Return-Path: {johnsonloretta061voaz-at-gmail.com} Received: from gmail.com (a122.designerforumail.com [157.52.193.122] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 04LLJtRY019972 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 21 May 2020 16:19:56 -0500 Received: from unknown (HELO mmx09.tilkbans.com) (Fri, 22 May 2020 03:13:51 +0600) by mxs.perenter.com with SMTP; Fri, 22 May 2020 03:13:51 +0600 Received: from qnx.mdrost.com [108.128.234.180] by relay37.vosimerkam.net with SMTP; Fri, 22 May 2020 03:00:43 +0600 Message-ID: {24B93BF1.B487FE89-at-gmail.com}
X-from: vitha-at-tamu.edu
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Email: vitha-at-tamu.edu
Name: Stanislav Vitha
Organization: Texas A&M University
Title-Subject: [Filtered] Eyepiece cameras for TEM as a focusing aid/demo tool?
Message: I was wondering if anybody has an experience or opinion on using an eyepiece camera (it would replace one of the eyepieces in the focusing binoculars on the TEM) as an aid or a demonstration/training tool on TEMs that do not have the live view option for their imaging cameras.
I could see it being used when demonstrating astigmatism (and stigmation), and focusing. There are some monochrome eyepiece cameras that are used for hobby astrophotography. Is it a bad idea or could it actually be useful?
Thank you!
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Do you have a digital camera on your TEM? Even the lower end ones should be able to generate a frame rate adequate for illustrative movies. You can work at frame rates as low as 4 frames/sec and still be able to see the process. You can also reduce your pixel resolution (512x512te is adequate) on the camera (binning) to increase the frame rate if necessary.
I've done this with a camera that didn't have movie recording capability and I couldn't install a recording program on the instrument PC. I had, however, a program that allowed me to record the screen on my laptop. I used VNC to remote into my TEM PC and then recorded my laptop screen.
God luck, Henk
----------------
Hendrik O. Colijn Center forElectronMicroscopy andAnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, May 21, 2020 7:51 PM To: Colijn, Hendrik {colijn.1-at-osu.edu}
Dear All,
We are looking for a used CM-series or Tecnai-series FEI microscope that someone wants to decommission.
The TEM will be used in our laboratory for beginner training purpose and to use as a backup machine for basic imaging purpose. The costs of transport will be covered by an intermediate company that we have contacted and the microscope can be in Europe, United States of America or any other part of the world.
Please message me personally if you are planning to offer such a microscope.
Dr. Surya Snata Rout M-26, Betriebseinheit Elektronenmikroskopie Technische Universität Hamburg (Hamburg University of Technology) Eißendorfer Straße 42 21073 Hamburg Germany
Tel.: +49 (0) 40 - 428 78 4881
==============================Original Headers============================== 13, 35 -- From surya.rout-at-tuhh.de Fri May 22 06:19:46 2020 13, 35 -- Received: from smtp1.rz.tu-harburg.de (smtp1.rz.tu-harburg.de [134.28.205.38]) 13, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 04MBJjqW031520 13, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2020 06:19:46 -0500 13, 35 -- Received: from mail.tu-harburg.de (mail4.rz.tu-harburg.de [134.28.202.83]) 13, 35 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 13, 35 -- (Client CN "mail.tuhh.de", Issuer "DFN-Verein Global Issuing CA" (verified OK)) 13, 35 -- by smtp1.rz.tu-harburg.de (Postfix) with ESMTPS id 49T3y64JVTzxVV 13, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2020 13:23:10 +0200 (CEST) 13, 35 -- Received: from mailspring.rz.tuhh.de (mailspring.rz.tuhh.de [134.28.202.181]) 13, 35 -- (using TLSv1 with cipher ECDHE-RSA-AES256-SHA (256/256 bits)) 13, 35 -- (No client certificate requested) 13, 35 -- (Authenticated sender: csr6105-at-KERBEROS.TU-HARBURG.DE) 13, 35 -- by mail.tu-harburg.de (Postfix) with ESMTPSA id 49T3y6450gzJrC5 13, 35 -- for {Microscopy-at-microscopy.com} ; Fri, 22 May 2020 13:23:10 +0200 (CEST) 13, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=tuhh.de; s=x2020-21; 13, 35 -- t=1590146590; bh=p+NNRmPdJ276sDFhRYOqvWSNCwQKHeZEcZGSFHhx3bY=; 13, 35 -- h=Date:Message-ID:From:To:Subject:Content-Type:MIME-Version: 13, 35 -- Content-Transfer-Encoding; 13, 35 -- b=FfmZMkDkDWyCt6qofB5fsZzixUuTB+aebfWoKXa1ln6YLhG0kN3twB4OAyuPw3TaO 13, 35 -- NpwT5tWeblwwgcU7TeW9tpm9lZNYHbtYuVvVjnhhfVI/8JX+2VkCsDY2v1VTYKgRIy 13, 35 -- F2DyKpUOGiitAlJlCs8rufGE+eD3sgAMQy/h8YTg= 13, 35 -- Received: from x4d011e6a.dyn.telefonica.de (x4d011e6a.dyn.telefonica.de 13, 35 -- [77.1.30.106]) by webmail.tu-harburg.de (Horde Framework) with HTTPS; Fri, 13, 35 -- 22 May 2020 13:23:10 +0200 13, 35 -- Date: Fri, 22 May 2020 13:23:10 +0200 13, 35 -- Message-ID: {20200522132310.Horde.gRJoM8SBH1rpIKGsLjxyxwZ-at-webmail.tu-harburg.de} 13, 35 -- From: Surya Snata Rout {surya.rout-at-tuhh.de} 13, 35 -- To: Microscopy-at-microscopy.com 13, 35 -- Subject: Looking For Used FEI (CM-series) TEM (TU Hamburg) 13, 35 -- User-Agent: Horde Application Framework 5 13, 35 -- Content-Type: text/plain; charset=utf-8; format=flowed; DelSp=Yes 13, 35 -- MIME-Version: 1.0 13, 35 -- Content-Disposition: inline 13, 35 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
It can be a good idea, yes. FEI sold a green screen cover with integrated Logitech webcam as part of the TARO (Tecnai advanced remote operation) package, and it can be used for the purpose you described. We have a bottom mount digital so we just use that. An eyepiece camera should work, assuming you can match the focal length to the optics of the stereoscope or get an adjustable one. Ideally you can get one that easily pops off and on, or work with your machine shop to make a quick change adapter. You don't realize how often you use the little screen and binoculars until you can't.
As Henk mentioned, you can simulate a live view by binning your camera down and snapping pictures at very low exposure times. You can use a mouse control macro on Windows to automatically click the capture button, and even automatically generate a FFT from each image.
For aberrations in STEM mode, there are simulation packages that can also be used to show aberrations in the Ronchigram like Dr. Probe. I really enjoy these as training tools as you can adjust them, making them worse then better, so the users can see how aberrations affect the Ronchigram and play around offline. You are probably talking about TEM mode, though, so maybe not so useful.
When I was in charge of an EM420 with no live view camera, I got lazy and just adjusted objective stig and told users not to change it. It was stable for many weeks and we did not have the budget for a live camera or even eyepiece camera. Focusing was pretty easy to demonstrate without the live view.
Good luck, Chris
On Thu, May 21, 2020 at 7:56 PM {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: vitha-at-tamu.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } vitha-at-tamu.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: vitha-at-tamu.edu } } Name: Stanislav Vitha } } Organization: Texas A&M University } } Title-Subject: [Filtered] Eyepiece cameras for TEM as a focusing aid/demo tool? } } Message: I was wondering if anybody has an experience or opinion on using an eyepiece camera (it } would replace one of the eyepieces in the focusing binoculars on the TEM) as an aid or a } demonstration/training tool on TEMs that do not have the live view option for their imaging cameras. } } I could see it being used when demonstrating astigmatism (and stigmation), and focusing. There are } some monochrome eyepiece cameras that are used for hobby astrophotography. } Is it a bad idea or could it actually be useful? } } Thank you! } } Stan Vitha } Login Host: 128.194.151.97 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 11, 53 -- From microscopy.listserver-at-gmail.com Thu May 21 18:45:58 2020 } 11, 53 -- Received: from mail-il1-f172.google.com (mail-il1-f172.google.com [209.85.166.172]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 04LNjwix032710 } 11, 53 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2020 18:45:58 -0500 } 11, 53 -- Received: by mail-il1-f172.google.com with SMTP id j3so8964252ilk.11 } 11, 53 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2020 16:49:23 -0700 (PDT) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=PuXgvFrpQAf3jqelHiMtUivldewJhqojKV8Rnio16Rg=; } 11, 53 -- b=OlvWf2cGLnzeobxQn/jmKZjx7aRbFYwrWQanZPlPQa/lWnXhuQ1OndCYKQmVDk/Uny } 11, 53 -- qx8aG6CU6CI0+2z5YvNUrNsKa8h68z5WyzxuBHogVSrB6COxrhuYitmqr2l6O/SSMY0w } 11, 53 -- Ol+pBI7MYBnm+0DkM/P7jRYSNJ/hugjHWzbPECMPKKuzXR+Svot5RjjbhcwKsg7oeFbM } 11, 53 -- JYJwtwL1R9apn0jv7BShj/StqNfffIK1Y8BVvkseVWIwCi4V9Kk1uYSv3IhLPHM0HJBg } 11, 53 -- Ew+6nlSxseZdPZ0PNIQ0a0oP9jKs+NrrXyu6lpal+Bdo+UhqoIQZ0DZaFLwWwXxsMJyA } 11, 53 -- nltw== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=PuXgvFrpQAf3jqelHiMtUivldewJhqojKV8Rnio16Rg=; } 11, 53 -- b=o7LeCoKntJJsrd+uQLwkpn3qF/dQINGgF8uKEFDCmWxKe8efOAKB2NUWa9K/bjz2QI } 11, 53 -- nj+uN9cSAGMn2McrOPRsrYBp7X+gWxiCtY7QMaIkJJ9NNZu8O9EA/lh5i9Y5RB89V7cW } 11, 53 -- M2f59YG6YkFyJkd/TbXqBN1fGTikzZ3KbeXVi1+XspNQAOZicV8ZIOxPo2athAU5KlSQ } 11, 53 -- KcnxYp/9lOKCCzwUnJlW5x9NqrQMIpqIPjAhClKHDesf5LrCb5INGp6VmQxQZyH4GrJl } 11, 53 -- cdYldFU8RcULsTDDMo+eeXSufYeDAU3FXFeHBBme1gi/3/74bkuD8rsOYIb8i6e+fgg3 } 11, 53 -- A/ww== } 11, 53 -- X-Gm-Message-State: AOAM533mtHxu9oqPDm3LTUy89ZbLkA77LI8aFbHKgWVYeUZ/6CWL9kiJ } 11, 53 -- YSQyA0BGWWsAWupNV5swVgtj3ZjG } 11, 53 -- X-Google-Smtp-Source: ABdhPJwsfXvMGuISqsFmaDFKN3V0ZBdht6c8/FsblS2wuW4iJzakTQKQdZ2d44V5xxEPuYyJ6TFdDA== } 11, 53 -- X-Received: by 2002:a92:c94f:: with SMTP id i15mr10294840ilq.185.1590104963116; } 11, 53 -- Thu, 21 May 2020 16:49:23 -0700 (PDT) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:5128:efc5:59bd:6bfa]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id f9sm3026975iow.47.2020.05.21.16.49.22 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 11, 53 -- Thu, 21 May 2020 16:49:22 -0700 (PDT) } 11, 53 -- Subject: viaWWW: Eyepiece cameras for TEM as a focusing aid/demo tool? } 11, 53 -- References: {202005211742.04LHgrdS006342-at-microscopy.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: {202005211742.04LHgrdS006342-at-microscopy.com} } 11, 53 -- Message-ID: {b903887d-3778-bc75-bdea-3ab7df740316-at-gmail.com} } 11, 53 -- Date: Thu, 21 May 2020 18:49:21 -0500 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 11, 53 -- Gecko/20100101 Thunderbird/68.8.0 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: {202005211742.04LHgrdS006342-at-microscopy.com} } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
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It would be ideal if such a camera could provide a real-time power spectrum. That would depend on the granularity of the viewing-screen phosphor and the magnification used.
On 5/21/2020 7:48 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: vitha-at-tamu.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } vitha-at-tamu.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: vitha-at-tamu.edu } } Name: Stanislav Vitha } } Organization: Texas A&M University } } Title-Subject: [Filtered] Eyepiece cameras for TEM as a focusing aid/demo tool? } } Message: I was wondering if anybody has an experience or opinion on using an eyepiece camera (it } would replace one of the eyepieces in the focusing binoculars on the TEM) as an aid or a } demonstration/training tool on TEMs that do not have the live view option for their imaging cameras. } } I could see it being used when demonstrating astigmatism (and stigmation), and focusing. There are } some monochrome eyepiece cameras that are used for hobby astrophotography. } Is it a bad idea or could it actually be useful? } } Thank you! } } Stan Vitha } Login Host: 128.194.151.97 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 11, 53 -- From microscopy.listserver-at-gmail.com Thu May 21 18:45:58 2020 } 11, 53 -- Received: from mail-il1-f172.google.com (mail-il1-f172.google.com [209.85.166.172]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 04LNjwix032710 } 11, 53 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2020 18:45:58 -0500 } 11, 53 -- Received: by mail-il1-f172.google.com with SMTP id j3so8964252ilk.11 } 11, 53 -- for {microscopy-at-microscopy.com} ; Thu, 21 May 2020 16:49:23 -0700 (PDT) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=PuXgvFrpQAf3jqelHiMtUivldewJhqojKV8Rnio16Rg=; } 11, 53 -- b=OlvWf2cGLnzeobxQn/jmKZjx7aRbFYwrWQanZPlPQa/lWnXhuQ1OndCYKQmVDk/Uny } 11, 53 -- qx8aG6CU6CI0+2z5YvNUrNsKa8h68z5WyzxuBHogVSrB6COxrhuYitmqr2l6O/SSMY0w } 11, 53 -- Ol+pBI7MYBnm+0DkM/P7jRYSNJ/hugjHWzbPECMPKKuzXR+Svot5RjjbhcwKsg7oeFbM } 11, 53 -- JYJwtwL1R9apn0jv7BShj/StqNfffIK1Y8BVvkseVWIwCi4V9Kk1uYSv3IhLPHM0HJBg } 11, 53 -- Ew+6nlSxseZdPZ0PNIQ0a0oP9jKs+NrrXyu6lpal+Bdo+UhqoIQZ0DZaFLwWwXxsMJyA } 11, 53 -- nltw== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=PuXgvFrpQAf3jqelHiMtUivldewJhqojKV8Rnio16Rg=; } 11, 53 -- b=o7LeCoKntJJsrd+uQLwkpn3qF/dQINGgF8uKEFDCmWxKe8efOAKB2NUWa9K/bjz2QI } 11, 53 -- nj+uN9cSAGMn2McrOPRsrYBp7X+gWxiCtY7QMaIkJJ9NNZu8O9EA/lh5i9Y5RB89V7cW } 11, 53 -- M2f59YG6YkFyJkd/TbXqBN1fGTikzZ3KbeXVi1+XspNQAOZicV8ZIOxPo2athAU5KlSQ } 11, 53 -- KcnxYp/9lOKCCzwUnJlW5x9NqrQMIpqIPjAhClKHDesf5LrCb5INGp6VmQxQZyH4GrJl } 11, 53 -- cdYldFU8RcULsTDDMo+eeXSufYeDAU3FXFeHBBme1gi/3/74bkuD8rsOYIb8i6e+fgg3 } 11, 53 -- A/ww== } 11, 53 -- X-Gm-Message-State: AOAM533mtHxu9oqPDm3LTUy89ZbLkA77LI8aFbHKgWVYeUZ/6CWL9kiJ } 11, 53 -- YSQyA0BGWWsAWupNV5swVgtj3ZjG } 11, 53 -- X-Google-Smtp-Source: ABdhPJwsfXvMGuISqsFmaDFKN3V0ZBdht6c8/FsblS2wuW4iJzakTQKQdZ2d44V5xxEPuYyJ6TFdDA== } 11, 53 -- X-Received: by 2002:a92:c94f:: with SMTP id i15mr10294840ilq.185.1590104963116; } 11, 53 -- Thu, 21 May 2020 16:49:23 -0700 (PDT) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:5128:efc5:59bd:6bfa]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id f9sm3026975iow.47.2020.05.21.16.49.22 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 11, 53 -- Thu, 21 May 2020 16:49:22 -0700 (PDT) } 11, 53 -- Subject: viaWWW: Eyepiece cameras for TEM as a focusing aid/demo tool? } 11, 53 -- References: {202005211742.04LHgrdS006342-at-microscopy.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: {202005211742.04LHgrdS006342-at-microscopy.com} } 11, 53 -- Message-ID: {b903887d-3778-bc75-bdea-3ab7df740316-at-gmail.com} } 11, 53 -- Date: Thu, 21 May 2020 18:49:21 -0500 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 11, 53 -- Gecko/20100101 Thunderbird/68.8.0 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: {202005211742.04LHgrdS006342-at-microscopy.com} } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
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We are looking for a staff member with expertise in TEM, cryo-TEM and electron tomography at the Bioimaging Facility at University of British Columbia. Link to job advertisement: https://www.hr.ubc.ca/careers-postings/staff-s.php Job ID # is 37507 Closing date: June 15th, 2020 Facility website: www.bioimaging.ubc.ca
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I am looking for a Pre-Installation Manual on a Philips/FEI/ThermoFisher CM10 TEM.
Does anybody have one available in PDF?
I would appreciate it very much.
Thanks,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
==============================Original Headers============================== 12, 24 -- From stefan.diller-at-t-online.de Wed May 27 03:32:49 2020 12, 24 -- Received: from mailout10.t-online.de (mailout10.t-online.de [194.25.134.21]) 12, 24 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 04R8WmUN006475 12, 24 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2020 03:32:49 -0500 12, 24 -- Received: from fwd17.aul.t-online.de (fwd17.aul.t-online.de [172.20.27.64]) 12, 24 -- by mailout10.t-online.de (Postfix) with SMTP id A9F9241A906A 12, 24 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2020 10:36:30 +0200 (CEST) 12, 24 -- Received: from mac-pro.local (JT4LAGZHrhUd80I5vke9tNjUHh+uc9xuWd5mT777wO-EP7KKcckFFCwDLgqgAOGgTQ-at-[31.16.253.92]) by fwd17.t-online.de 12, 24 -- with (TLSv1.2:ECDHE-RSA-AES256-GCM-SHA384 encrypted) 12, 24 -- esmtp id 1jdrY4-01Lruq0; Wed, 27 May 2020 10:36:28 +0200 12, 24 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 12, 24 -- From: Stefan Diller {stefan.diller-at-t-online.de} 12, 24 -- Subject: CM10 Pre-Installation Manual 12, 24 -- Message-ID: {189e486f-bba8-c7b4-0f4e-b77288cbadf9-at-t-online.de} 12, 24 -- Date: Wed, 27 May 2020 10:36:27 +0200 12, 24 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:68.0) 12, 24 -- Gecko/20100101 Thunderbird/68.8.1 12, 24 -- MIME-Version: 1.0 12, 24 -- Content-Type: text/plain; charset=windows-1252; format=flowed 12, 24 -- Content-Transfer-Encoding: 7bit 12, 24 -- Content-Language: en-GB 12, 24 -- X-ID: JT4LAGZHrhUd80I5vke9tNjUHh+uc9xuWd5mT777wO-EP7KKcckFFCwDLgqgAOGgTQ 12, 24 -- X-TOI-EXPURGATEID: 150726::1590568588-00006CDB-98847FDB/0/0 CLEAN NORMAL 12, 24 -- X-TOI-MSGID: 1207db31-4fd9-4517-9d42-c83a2374afd6 ==============================End of - Headers==============================
From buviregi380yjnbn-at-gmail.com Wed May 27 07:02:02 2020 Return-Path: {buviregi380yjnbn-at-gmail.com} Received: from gmail.com (a121.designerforumail.com [157.52.193.121] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 04RC21Ev027507 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 27 May 2020 07:02:02 -0500 Message-ID: {D3672C55.685A55AE-at-gmail.com}
Thanks, I got a CM100 Pre-Installation manual.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 27.05.20 um 10:39 schrieb stefan.diller-at-t-online.de: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All, } } I am looking for a Pre-Installation Manual on a Philips/FEI/ThermoFisher CM10 TEM. } } Does anybody have one available in PDF? } } I would appreciate it very much. } } } Thanks, } } Stefan }
==============================Original Headers============================== 7, 30 -- From diller-at-stefan-diller.com Wed May 27 10:56:36 2020 7, 30 -- Received: from mailout010.rox.net (mailout010.rox.net [212.63.85.210]) 7, 30 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 04RFuZCu009433 7, 30 -- for {microscopy-at-microscopy.com} ; Wed, 27 May 2020 10:56:35 -0500 7, 30 -- Received: from localhost ([127.0.0.1]) 7, 30 -- by mailout01.rox.net with esmtp (Exim 4.80) 7, 30 -- (envelope-from {diller-at-stefan-diller.com} ) 7, 30 -- id 1jdyTa-0006Nh-Bf 7, 30 -- for microscopy-at-microscopy.com; Wed, 27 May 2020 18:00:18 +0200 7, 30 -- Received: from [31.16.253.92] (helo=mac-pro.local) 7, 30 -- by mailout01.rox.net with esmtpsa (TLSv1.2:DHE-RSA-AES128-SHA:128) 7, 30 -- (Exim 4.80) 7, 30 -- (envelope-from {diller-at-stefan-diller.com} ) 7, 30 -- id 1jdyTa-0006Nb-5z 7, 30 -- for microscopy-at-microscopy.com; Wed, 27 May 2020 18:00:18 +0200 7, 30 -- Subject: Re: [Microscopy] CM10 Pre-Installation Manual - Solved 7, 30 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 30 -- References: {202005270839.04R8dQqv013421-at-microscopy.com} 7, 30 -- From: stefan diller {diller-at-stefan-diller.com} 7, 30 -- Message-ID: {7b8b5b72-c6bd-d5c2-4e94-6164e76e2c0c-at-stefan-diller.com} 7, 30 -- Date: Wed, 27 May 2020 18:00:17 +0200 7, 30 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:68.0) 7, 30 -- Gecko/20100101 Thunderbird/68.8.1 7, 30 -- MIME-Version: 1.0 7, 30 -- In-Reply-To: {202005270839.04R8dQqv013421-at-microscopy.com} 7, 30 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 30 -- Content-Transfer-Encoding: 7bit 7, 30 -- Content-Language: en-GB 7, 30 -- X-Envelope-From: {diller-at-stefan-diller.com} 7, 30 -- X-Scanned-By: rockenstein AG ==============================End of - Headers==============================
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Title-Subject: [Filtered] Ultrastructural differences between carcinoma and sarcoma cells.
Message: Can anyone help me to find the difference between carcinoma and sarcoma cells under TEM and SEM?
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Message: Dear all, The webinar: Imaging SARS-CoV-2 safely: Protecting the microscopy community is now available on demand here the link:
https://www.crick.ac.uk/whats-on/webinarimaging-sars-cov-2-safely-protecting-the-microscopy-community Best wishes, Raffa
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Our Vice Chancellor of Research asked us to develop metrics to evaluate productivity and growth of our electron microscopy core facility. We would like to hear if you have developed specific metrics to assess operations of your facility and what you have learned about the utility and value of different metrics. We appreciate input from any facility, even if you have not established metrics for your program.
Tom Bargar Electron Microscopy Specialist UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 tbargar-at-unmc.edu 402-559-7347
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We have a local physics laboratory that might be interested in acquiring a used TEM or SEM (working condition including computer support) to test novel electron sources and develop new electron imaging modalities. We would like to know if anyone has an instrument available in storage or is preparing to decommission an electron microscope.
Tom Bargar Electron Microscopy Specialist UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 tbargar-at-unmc.edu 402-559-7347
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
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Title-Subject: [Filtered] 2 PhD positions in Liquid Phase Electron Microscopy - apply before 21st June
Message: Are you or someone you know intersted in t studying nanoscale processes live in the liquid phase?
We have two PhD positions with international collaboration open where you can apply before 21st June 2020:
PhD scholarship in Nanochannel Liquid Cells for in situ Biochemical Phase Change Studies, in collaboration with Nanyang Tech. Uni. Singapore. https://www.nanolab.dtu.dk/english/aboutus/organisation/vacant-positions/job?id=b856978d-3f3b-4f92-8558-03a36138b9c0
PhD scholarship in Charges and Potentials in Liquid Phase Electron Microscopy, in collaboration with the Ernst-Ruska Center in Germany and Paul Alivisatos' group at UC Berkeley:
From doripama101ja-at-gmail.com Fri Jun 5 10:56:01 2020 Return-Path: {doripama101ja-at-gmail.com} Received: from gmail.com (a87.designerforumail.com [157.52.193.87] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 055Fu1wQ001807 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 5 Jun 2020 10:56:01 -0500 Message-ID: {A6BABEA1.606D677B-at-gmail.com}
Dear All We have a puzzling image problem with our JSM 5600LV SEM: We achieve a decent image at say X20K; then we lower the magnification at X50 or beyond and keep it there for about minute and then return it to X20K; now the image is very fussy and there is no way to clear it with focus or stigma; but if we wait for about 5 minutes without doing anything the image clears up by itself.
We have also noticed that when we are at high mag with clear image and apply stigma reset the stigma shifts too much and needs many button turns to get corrected.
Since we have a second working JSM 5600LV with same software version (2.05) we exchanged all boards between them but there was no effect on either microscope, so we think the problem is not there. Also we open the column of the problematic scope but couldnt see anything wrong.
Any idea of what could be the problem will be greatly appreciated Best regards yorgos
first: Had this happened at high-vac condition? Did you check the effect on another sample? There might be outgassing or an effect of specimen preparation.
Another thing could be the beam situation at low and high mag: different diameter with the chance that the more intense low mag beam might produce charging in the scope inliner.
And there is the good chance the the photomultiplier tube / scintillator has seen "too much light". Normally this shows up as more noise, streaky patterns in the image.
I would do a check-up with a non-biological specimen, check at some kx magnification with a larger spotsize setting, and see if you can reproduce the effect at another set of magnifications.
If yes, you should next clean the inliner and change apertures. And / or change the SE detector head.
Best,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 08.06.20 um 08:01 schrieb eikonika-at-otenet.gr: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear All } We have a puzzling image problem with our JSM 5600LV SEM: We achieve a } decent image at say X20K; then we lower the magnification at X50 or beyond } and keep it there for about minute and then return it to X20K; now the } image is very fussy and there is no way to clear it with focus or stigma; } but if we wait for about 5 minutes without doing anything the image clears } up by itself. } } We have also noticed that when we are at high mag with clear image and apply } stigma reset the stigma shifts too much and needs many button turns to get } corrected. } } Since we have a second working JSM 5600LV with same software version (2.05) } we exchanged all boards between them but there was no effect on either } microscope, so we think the problem is not there. Also we open the column of } the problematic scope but couldnt see anything wrong. } } Any idea of what could be the problem will be greatly appreciated } Best regards } yorgos } } Dr Yorgos Nikas } Athens Innovative Microscopy } Skra 36 Voula 16673 GREECE } } Tel/fax +30 210 8957677 } mobile +30 6945 107477 } www.eikonika.netwww.aim.cat } ************************************* } } } } } ==============================Original Headers============================== } 8, 21 -- From eikonika-at-otenet.gr Mon Jun 8 00:53:39 2020 } 8, 21 -- Received: from hydra.otenet.gr (hydra.otenet.gr [83.235.69.20]) } 8, 21 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0585rdW6016179 } 8, 21 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jun 2020 00:53:39 -0500 } 8, 21 -- Received: from ozymandias (ppp-2-84-175-154.home.otenet.gr [2.84.175.154]) } 8, 21 -- (Authenticated sender: eikonika-at-otenet.gr) } 8, 21 -- by hydra.otenet.gr (ESMTP) with ESMTPSA } 8, 21 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jun 2020 08:57:48 +0300 (EEST) } 8, 21 -- From: "Yorgos Nikas" {eikonika-at-otenet.gr} } 8, 21 -- To: {microscopy-at-microscopy.com} } 8, 21 -- Subject: JSM 5600LV SEM fussy image problem } 8, 21 -- Date: Mon, 8 Jun 2020 08:57:44 +0300 } 8, 21 -- Message-ID: {000601d63d59$bbe29260$33a7b720$-at-otenet.gr} } 8, 21 -- MIME-Version: 1.0 } 8, 21 -- Content-Type: text/plain; } 8, 21 -- charset="iso-8859-1" } 8, 21 -- X-Mailer: Microsoft Outlook 14.0 } 8, 21 -- Thread-Index: AdY9WYihXlUhs1BYSX6IQmPfsrzvug== } 8, 21 -- Content-Language: en-us } 8, 21 -- Content-Transfer-Encoding: 8bit } 8, 21 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 0585rdW6016179 } ==============================End of - Headers==============================
==============================Original Headers============================== 14, 31 -- From diller-at-stefan-diller.com Mon Jun 8 01:45:49 2020 14, 31 -- Received: from mailout010.rox.net (mailout010.rox.net [212.63.85.210]) 14, 31 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0586jm9C028193 14, 31 -- for {microscopy-at-microscopy.com} ; Mon, 8 Jun 2020 01:45:48 -0500 14, 31 -- Received: from localhost ([127.0.0.1]) 14, 31 -- by mailout01.rox.net with esmtp (Exim 4.80) 14, 31 -- (envelope-from {diller-at-stefan-diller.com} ) 14, 31 -- id 1jiBbl-0007tH-Iw; Mon, 08 Jun 2020 08:50:09 +0200 14, 31 -- Received: from ip1f10fd5c.dynamic.kabel-deutschland.de ([31.16.253.92] helo=mac-pro.local) 14, 31 -- by mailout01.rox.net with esmtpsa (TLSv1.2:DHE-RSA-AES128-SHA:128) 14, 31 -- (Exim 4.80) 14, 31 -- (envelope-from {diller-at-stefan-diller.com} ) 14, 31 -- id 1jiBbl-0007t8-C9; Mon, 08 Jun 2020 08:50:09 +0200 14, 31 -- Subject: Re: [Microscopy] JSM 5600LV SEM fussy image problem 14, 31 -- To: eikonika-at-otenet.gr, 14, 31 -- "Microscopy-at-microscopy.com" 14, 31 -- {microscopy-at-microscopy.com} 14, 31 -- References: {202006080601.05861uIj023388-at-microscopy.com} 14, 31 -- From: stefan diller {diller-at-stefan-diller.com} 14, 31 -- Message-ID: {33de6061-e147-ef82-d343-0cb3050f140a-at-stefan-diller.com} 14, 31 -- Date: Mon, 8 Jun 2020 08:50:08 +0200 14, 31 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:68.0) 14, 31 -- Gecko/20100101 Thunderbird/68.9.0 14, 31 -- MIME-Version: 1.0 14, 31 -- In-Reply-To: {202006080601.05861uIj023388-at-microscopy.com} 14, 31 -- Content-Type: text/plain; charset=windows-1252; format=flowed 14, 31 -- Content-Language: en-GB 14, 31 -- X-Envelope-From: {diller-at-stefan-diller.com} 14, 31 -- X-Scanned-By: rockenstein AG 14, 31 -- Content-Transfer-Encoding: 8bit 14, 31 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 0586jm9C028193 ==============================End of - Headers==============================
-----Original Message----- X-from: diller-at-stefan-diller.com {diller-at-stefan-diller.com} Sent: Monday, June 8, 2020 8:52 AM To: Houet, Jack {jack.houet-at-thermofisher.com}
Yorgos -
I suspect you have something in the beam path that receives charge at low magnification because of the wide sweep at low mag.
However, I do not know enough about the JSM 5600LV to give suggestions on cleaning the column.
However, I would suggest trying to put in a very large metal sample, to eliminate the charging on the sample holder. Also, double check the grounding on the sample holder.
regards, - Jim
==============================Original Headers============================== 5, 35 -- From james.quinn-at-stonybrook.edu Mon Jun 8 09:48:12 2020 5, 35 -- Received: from mail-ot1-f48.google.com (mail-ot1-f48.google.com [209.85.210.48]) 5, 35 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 058EmC59011896 5, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 8 Jun 2020 09:48:12 -0500 5, 35 -- Received: by mail-ot1-f48.google.com with SMTP id k15so13865809otp.8 5, 35 -- for {Microscopy-at-microscopy.com} ; Mon, 08 Jun 2020 07:52:35 -0700 (PDT) 5, 35 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 35 -- d=stonybrook.edu; s=sbu-gmail; 5, 35 -- h=mime-version:from:date:message-id:subject:to; 5, 35 -- bh=n+9lhwyX4ncbfDfjLuAm8hC7OM97iTwvU4lRr+r+IOs=; 5, 35 -- b=cIvTd8G8fryhddhmhy9S1b6T47pKw+KvrWixVrs7aztUhxunnYq8p7fa7HqCKVB+P2 5, 35 -- 8g+bpFuJuttKHpRwNrFSqwZJwlVFKvyzmtYL852+AGTTtgI5fdJYN+G52sqGHplh+oDj 5, 35 -- x3Az+Y8b3RaRRyFE0Id6D1kWo9DJUU0Qvqlvw= 5, 35 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 5, 35 -- d=1e100.net; s=20161025; 5, 35 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 5, 35 -- bh=n+9lhwyX4ncbfDfjLuAm8hC7OM97iTwvU4lRr+r+IOs=; 5, 35 -- b=ljKDOWGlxsxNmPsgQiInob2wVKZ2RcFhH0hg2dqAz+El7tHwO4YhyLDMHUTgdrW8c4 5, 35 -- hEs7GaVIY11wYYfKU83rFY4mNLG5ZsQEQ1SnvuGUVGy/NFeoRZj16sExfCiBFs5kq6dS 5, 35 -- AIZ0QFzx5w7LpmksBr1Xc/6n/tFaLUszh+ri7IzU89RI/pMRZxLYHisaeKpv4FR9zTI1 5, 35 -- ak0LtkW0CdIeu4L8ip8N98q4XPSfrs7FoWpHlIl9nZNU4dvQ+n8S/v1EthAw0JpY4lsC 5, 35 -- QXNo+L167DamkDOOizzbKmRyk+FWg3XO9o3D2y/25P8XAZoRShx0vdALRg8KhTU3FajL 5, 35 -- otGA== 5, 35 -- X-Gm-Message-State: AOAM53008Wl0N2oyK0peDr9QymxNe0b5GnzBPthx7x/A6xE95VzLqBG0 5, 35 -- +NpHgC+m+f9aEedHeCBc1uPLiMmRrBTfZEIhb2EVKIBISSM= 5, 35 -- X-Google-Smtp-Source: ABdhPJyfNvZmdg/IhxgZ7rS+vrSfIHFPOvc31piwciom6XSSITgLivRCt6YbIW7Ahm4bamAK3C1dF0t10Xd0RZJbSVM= 5, 35 -- X-Received: by 2002:a9d:665a:: with SMTP id q26mr15561889otm.174.1591627954657; 5, 35 -- Mon, 08 Jun 2020 07:52:34 -0700 (PDT) 5, 35 -- MIME-Version: 1.0 5, 35 -- From: Jim Quinn {james.quinn-at-stonybrook.edu} 5, 35 -- Date: Mon, 8 Jun 2020 10:52:24 -0400 5, 35 -- Message-ID: {CAMtM03HzwcxrSd2JC2bcu_NTQdRP=8TRTAaw4UYMaZpUdskAKQ-at-mail.gmail.com} 5, 35 -- Subject: re: JSM 5600LV SEM fussy image problem 5, 35 -- To: Microscopy-at-microscopy.com 5, 35 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
From jeverett397guhno-at-gmail.com Mon Jun 8 19:58:50 2020 Return-Path: {jeverett397guhno-at-gmail.com} Received: from gmail.com (a110.designerforumail.com [157.52.193.110] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 0590wnrO023255 for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 8 Jun 2020 19:58:50 -0500 Message-ID: {38255F0B.81CFAE62-at-gmail.com}
X-from: alice.dohnalkova-at-pnnl.gov
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Email: alice.dohnalkova-at-pnnl.gov
Name: Alice Dohnalkova
Organization: Pacific Northwest National Laboratory
Title-Subject: [Filtered] Leica EM Trim schematics?
Message: Dear fellow microscopists,
My lab has a Leica EM Trim tool (the 'old' one, not EM Trim2), and the advance wheel on the front has stopped working. I will ask our machine shop guys to fix it, but it would be great to have the EM Trim schematics ahead for them.
I realize this is a 20+ year old instrument, but if anyone still kept the brochure, I'd really appreciate your help sharing it.
Best regards, Alice.
Alice Dohnalkova, Ph.D. ENVIRONMENTAL MOLECULAR SCIENCES LABORATORY Pacific Northwest National Laboratory 902 Battelle Boulevard P.O. Box 999, MSIN K8-93 Richland, WA 99352 USA Tel: 509-371-6515
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Hi again Thank you Stefan, Jim, Warren, and Robert for your help to solve this problem. With your wise advice and the power of having a twin system I could reliably check the suggested points and narrow the problem down to the beam path. Indeed after cleaning the objective lens aperture (there were some traces of oil on it) the SEM works very well already several hours now and with a bit of luck will stay so. There is still a minute drift of stigmatism barely noticeable but doesn't seem to be an ominous sign. All the best yorgos
-----Original Message----- X-from: Yorgos Nikas [mailto:eikonika-at-otenet.gr] Sent: Monday, June 8, 2020 08:58 To: 'microscopy-at-microscopy.com'
X-from: arunasme-at-gmail.com
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Email: arunasme-at-gmail.com Name: Arunas
Title-Subject: [Filtered] Principle of how the SAED pattern is formed
Message: Hello,
Could someone please explain to me the principle of how is a selected area diffraction pattern formed inside the TEM column?
I understand the principles by which diffractograms from x-ray powder diffraction (XRD) are obtained. X-ray Wavelengths, inter-planar spacings and constructive/destructive interferences.
However, I simply cannot wrap my head around the SAED pattern, how are they obtained? How is it that its possible to get a pattern from a 2D material such a sheet of graphene? Shouldn't there be at least two layers to generate constructive interference (hexagonal dot pattern)? I magine the beam hitting the graphene sheet perpendicularly while the sheet is perfectly horizontal on the TEM grid. The scatter points (atoms) would scatter the electrons in all directions equally without a particular preference, yet we still can see intensity maxima in the shape of a hexagonal pattern. I realize there must be a visual/logical flaw in how I imagine this process, any help to explain/visualize the real situation would be welcome. I've gone through materials online, however it seems the explanations lack visual representation and are very trigonometry heavy - that is not helping me to picture the situation in my head.
Regards,
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X-from: Ron Anderson {randerson20-at-tampabay.rr.com}
Arunas,
With one exception, your understanding of the scattering/diffraction process is correct. The one exception is the assumption that the thin graphene specimen on the grid is one atom layer thick. Not true. If you are seeing a sharp, low background, hexagonal pattern on the screen the sample is at least 10nm thick, which translates to 30 or so atomic planes thick. The atoms in each plane scatter the electrons in all directions. All of these spheres of scattered electrons encounter electron spheres from their neighbors with the result that most scattering directions are cancelled out. Only those scattering directions that satisfy Bragg's Law will allow for the passage of a diffracted beam through the specimen, which results in the observed diffraction pattern.
Ron Anderson, Happily retired in Florida but keeping an eye of the listserver
On 6/10/2020 10:08 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: arunasme-at-gmail.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } arunasme-at-gmail.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: arunasme-at-gmail.com Name: Arunas } } Title-Subject: [Filtered] Principle of how the SAED pattern is formed } } Message: Hello, } } Could someone please explain to me the principle of how is a selected area diffraction pattern } formed inside the TEM column? } } I understand the principles by which diffractograms from x-ray powder diffraction (XRD) are } obtained. X-ray Wavelengths, inter-planar spacings and constructive/destructive interferences. } } However, I simply cannot wrap my head around the SAED pattern, how are they obtained? How is it that } its possible to get a pattern from a 2D material such a sheet of graphene? Shouldn't there be at } least two layers to generate constructive interference (hexagonal dot pattern)? I magine the beam } hitting the graphene sheet perpendicularly while the sheet is perfectly horizontal on the TEM grid. } The scatter points (atoms) would scatter the electrons in all directions equally without a } particular preference, yet we still can see intensity maxima in the shape of a hexagonal pattern. } I realize there must be a visual/logical flaw in how I imagine this process, any help to } explain/visualize the real situation would be welcome. I've gone through materials online, however } it seems the explanations lack visual representation and are very trigonometry heavy - that is not } helping me to picture the situation in my head. } } Regards, } } Login Host: 193.167.228.180 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 12, 53 -- From microscopy.listserver-at-gmail.com Wed Jun 10 21:07:25 2020 } 12, 53 -- Received: from mail-io1-f51.google.com (mail-io1-f51.google.com [209.85.166.51]) } 12, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 05B27OFd016661 } 12, 53 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2020 21:07:25 -0500 } 12, 53 -- Received: by mail-io1-f51.google.com with SMTP id d5so4593738ios.9 } 12, 53 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2020 19:11:56 -0700 (PDT) } 12, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 53 -- d=gmail.com; s=20161025; } 12, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 12, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 12, 53 -- bh=z6VuXgUHN9hLzjoKseQ5cK5q+piL2H3xJ03wiJciflQ=; } 12, 53 -- b=II9VbKvceuQ+lxbd1Q/mUw784i7xFSQsWNwnqMj3O5HipxCUunEwer5BOnuffiNNsL } 12, 53 -- IaYAqktPu8ue6RCXb6/JnuiPyTkQCg7zKzQblKS/VGLBvki/XWStnhCoYfzv3vV4KsT7 } 12, 53 -- NglgGlcleJTiV7GPwbBUAce4De0G2N7NK/fTgLGd+gcrv4r25OL+fcdNmbqBcsvupG7T } 12, 53 -- 6plflxpjWITSGk0HH1+whOP/ekjF+7a9RjgxeFMgmitDf4DyK4XEBNIy1Vw0Q6i5oZs5 } 12, 53 -- uyMCGxr2SUuMMzV4TJQmenVrieSM86nnfdztd70zoNyrZ9HsEPySSBX+Wca4y6V+N8uB } 12, 53 -- F7Jw== } 12, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 53 -- d=1e100.net; s=20161025; } 12, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 12, 53 -- :user-agent:mime-version:in-reply-to:content-language } 12, 53 -- :content-transfer-encoding; } 12, 53 -- bh=z6VuXgUHN9hLzjoKseQ5cK5q+piL2H3xJ03wiJciflQ=; } 12, 53 -- b=terdINbtcMOOjJmDEUo9zYtJau+L0mHGp5SPG1+JQ92mgFNwTBlENYulFo7KCTBetE } 12, 53 -- hcIyJNpNIh4p4sABlJ/TIQ4KWE8kyXhh2oqlA6KVdZyCJrWzaVDo0vEGVrhM921Lh/SB } 12, 53 -- EYiL3/aDEAHPudIaSQX7B+87bfuN4LownRFiQJ3syuTzgfYQdrBAm+JUV7VvbaZvR2Td } 12, 53 -- 8JfRewVkWFklh8fbzBUXmq5h/T0xsZO2cflqE28z+x2YSe3giuEOOhyBc0bOkjJs4Sa2 } 12, 53 -- oyTIddtD87712t4ZKFU5xhXpn/rLgxS/bGCyM3S58R3MpgOwljYw+RnUsphYfaiCUCh+ } 12, 53 -- Nm7g== } 12, 53 -- X-Gm-Message-State: AOAM530eS76CkVPdHemUW+k4R/iYJ9SQ4uK2AUFcn/iz1sfIZxrRO99g } 12, 53 -- fClgjQTaAJDJgJslUBFXWQ41bX0h } 12, 53 -- X-Google-Smtp-Source: ABdhPJyLM4OyI0Jz4hoqNkzfsYddNyDQTRsD38vkGivBJViSnkszWTTsDIGWixIDMhvqOPS2fq4+3w== } 12, 53 -- X-Received: by 2002:a5d:9e55:: with SMTP id i21mr6351876ioi.130.1591841515147; } 12, 53 -- Wed, 10 Jun 2020 19:11:55 -0700 (PDT) } 12, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:3de2:3386:52e6:cafc]) } 12, 53 -- by smtp.googlemail.com with ESMTPSA id a2sm798598iln.38.2020.06.10.19.11.53 } 12, 53 -- for {microscopy-at-microscopy.com} } 12, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 12, 53 -- Wed, 10 Jun 2020 19:11:54 -0700 (PDT) } 12, 53 -- Subject: viaWWW: Principle of how the SAED pattern is formed } 12, 53 -- References: {202006100901.05A91m3d013813-at-microscopy.com} } 12, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 12, 53 -- X-Forwarded-Message-Id: {202006100901.05A91m3d013813-at-microscopy.com} } 12, 53 -- Message-ID: {76cdd245-360f-80c5-b1e6-18fb6cf90e1b-at-gmail.com} } 12, 53 -- Date: Wed, 10 Jun 2020 21:11:53 -0500 } 12, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 12, 53 -- Gecko/20100101 Thunderbird/68.9.0 } 12, 53 -- MIME-Version: 1.0 } 12, 53 -- In-Reply-To: {202006100901.05A91m3d013813-at-microscopy.com} } 12, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 12, 53 -- Content-Language: en-US } 12, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
I suspect that you are picturing X-ray diffraction in reflection. In that case, you are looking at the planes parallel to the surface and, indeed, one plane won't give diffraction. In TEM, we are working in transmission. Each atom becomes a scattering center. You can think of this in manner similar to Young's slit experiment. You have 2 slits and the waves from each slit interfere to form a diffraction pattern. In TEM, the atoms are scattering centers and act in a manner analogous to the slits. I hope this helps, Henk
----------------
Hendrik O. Colijn Center forElectronMicroscopy andAnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Wednesday, June 10, 2020 10:13 PM To: Colijn, Hendrik {colijn.1-at-osu.edu}
X-from: Philip Kck {koeck-at-kth.se}
Hi.
I would say you should treat a 2D crystal as a phase grating. You can find quite a lot about diffraction by phase gratings on the web and the book on Fourier optics by Goodman also covers it.
Here's an attempt at an explanation: If every point of the graphene sheet gives rise to a spherical wave (Huygens construction) then the relative phases of these waves will be different depending on whether a point contains an atom or not. You can only get constructive interference in directions where the Huygens waves are in phase. Those directions are the diffraction spots. In the direction of the spots the Huygens waves coming from the atoms interfere constructively with another and so do the waves coming from the gaps between the atoms.
Does that make sense?
Philip
________________________________________ Frn: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Skickat: den 11 juni 2020 04:16 Till: Philip Kck mne: [Microscopy] viaWWW: Principle of how the SAED pattern is formed
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Email: arunasme-at-gmail.com Name: Arunas
Title-Subject: [Filtered] Principle of how the SAED pattern is formed
Message: Hello,
Could someone please explain to me the principle of how is a selected area diffraction pattern formed inside the TEM column?
I understand the principles by which diffractograms from x-ray powder diffraction (XRD) are obtained. X-ray Wavelengths, inter-planar spacings and constructive/destructive interferences.
However, I simply cannot wrap my head around the SAED pattern, how are they obtained? How is it that its possible to get a pattern from a 2D material such a sheet of graphene? Shouldn't there be at least two layers to generate constructive interference (hexagonal dot pattern)? I magine the beam hitting the graphene sheet perpendicularly while the sheet is perfectly horizontal on the TEM grid. The scatter points (atoms) would scatter the electrons in all directions equally without a particular preference, yet we still can see intensity maxima in the shape of a hexagonal pattern. I realize there must be a visual/logical flaw in how I imagine this process, any help to explain/visualize the real situation would be welcome. I've gone through materials online, however it seems the explanations lack visual representation and are very trigonometry heavy - that is not helping me to picture the situation in my head.
Regards,
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I would say there are examples of single-layer crystals that give rise to electron diffraction. Most membrane protein crystals are only periodic in 2 directions and they diffract very nicely.
I would say 3D-periodicity or multi-layeredness is not necessary for diffraction. I wouldn't be surprised if a single graphene layer also gives a visible diffraction pattern.
All the best,
Philip ________________________________________ Frn: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Skickat: den 11 juni 2020 23:56 Till: Philip Kck mne: [Microscopy] Fwd: Re: viaWWW: Principle of how the SAED pattern is
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X-from: Ron Anderson {randerson20-at-tampabay.rr.com}
Arunas,
With one exception, your understanding of the scattering/diffraction process is correct. The one exception is the assumption that the thin graphene specimen on the grid is one atom layer thick. Not true. If you are seeing a sharp, low background, hexagonal pattern on the screen the sample is at least 10nm thick, which translates to 30 or so atomic planes thick. The atoms in each plane scatter the electrons in all directions. All of these spheres of scattered electrons encounter electron spheres from their neighbors with the result that most scattering directions are cancelled out. Only those scattering directions that satisfy Bragg's Law will allow for the passage of a diffracted beam through the specimen, which results in the observed diffraction pattern.
Ron Anderson, Happily retired in Florida but keeping an eye of the listserver
On 6/10/2020 10:08 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: arunasme-at-gmail.com } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } arunasme-at-gmail.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: arunasme-at-gmail.com Name: Arunas } } Title-Subject: [Filtered] Principle of how the SAED pattern is formed } } Message: Hello, } } Could someone please explain to me the principle of how is a selected area diffraction pattern } formed inside the TEM column? } } I understand the principles by which diffractograms from x-ray powder diffraction (XRD) are } obtained. X-ray Wavelengths, inter-planar spacings and constructive/destructive interferences. } } However, I simply cannot wrap my head around the SAED pattern, how are they obtained? How is it that } its possible to get a pattern from a 2D material such a sheet of graphene? Shouldn't there be at } least two layers to generate constructive interference (hexagonal dot pattern)? I magine the beam } hitting the graphene sheet perpendicularly while the sheet is perfectly horizontal on the TEM grid. } The scatter points (atoms) would scatter the electrons in all directions equally without a } particular preference, yet we still can see intensity maxima in the shape of a hexagonal pattern. } I realize there must be a visual/logical flaw in how I imagine this process, any help to } explain/visualize the real situation would be welcome. I've gone through materials online, however } it seems the explanations lack visual representation and are very trigonometry heavy - that is not } helping me to picture the situation in my head. } } Regards, } } Login Host: 193.167.228.180 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 12, 53 -- From microscopy.listserver-at-gmail.com Wed Jun 10 21:07:25 2020 } 12, 53 -- Received: from mail-io1-f51.google.com (mail-io1-f51.google.com [209.85.166.51]) } 12, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 05B27OFd016661 } 12, 53 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2020 21:07:25 -0500 } 12, 53 -- Received: by mail-io1-f51.google.com with SMTP id d5so4593738ios.9 } 12, 53 -- for {microscopy-at-microscopy.com} ; Wed, 10 Jun 2020 19:11:56 -0700 (PDT) } 12, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 53 -- d=gmail.com; s=20161025; } 12, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 12, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 12, 53 -- bh=z6VuXgUHN9hLzjoKseQ5cK5q+piL2H3xJ03wiJciflQ=; } 12, 53 -- b=II9VbKvceuQ+lxbd1Q/mUw784i7xFSQsWNwnqMj3O5HipxCUunEwer5BOnuffiNNsL } 12, 53 -- IaYAqktPu8ue6RCXb6/JnuiPyTkQCg7zKzQblKS/VGLBvki/XWStnhCoYfzv3vV4KsT7 } 12, 53 -- NglgGlcleJTiV7GPwbBUAce4De0G2N7NK/fTgLGd+gcrv4r25OL+fcdNmbqBcsvupG7T } 12, 53 -- 6plflxpjWITSGk0HH1+whOP/ekjF+7a9RjgxeFMgmitDf4DyK4XEBNIy1Vw0Q6i5oZs5 } 12, 53 -- uyMCGxr2SUuMMzV4TJQmenVrieSM86nnfdztd70zoNyrZ9HsEPySSBX+Wca4y6V+N8uB } 12, 53 -- F7Jw== } 12, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 53 -- d=1e100.net; s=20161025; } 12, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 12, 53 -- :user-agent:mime-version:in-reply-to:content-language } 12, 53 -- :content-transfer-encoding; } 12, 53 -- bh=z6VuXgUHN9hLzjoKseQ5cK5q+piL2H3xJ03wiJciflQ=; } 12, 53 -- b=terdINbtcMOOjJmDEUo9zYtJau+L0mHGp5SPG1+JQ92mgFNwTBlENYulFo7KCTBetE } 12, 53 -- hcIyJNpNIh4p4sABlJ/TIQ4KWE8kyXhh2oqlA6KVdZyCJrWzaVDo0vEGVrhM921Lh/SB } 12, 53 -- EYiL3/aDEAHPudIaSQX7B+87bfuN4LownRFiQJ3syuTzgfYQdrBAm+JUV7VvbaZvR2Td } 12, 53 -- 8JfRewVkWFklh8fbzBUXmq5h/T0xsZO2cflqE28z+x2YSe3giuEOOhyBc0bOkjJs4Sa2 } 12, 53 -- oyTIddtD87712t4ZKFU5xhXpn/rLgxS/bGCyM3S58R3MpgOwljYw+RnUsphYfaiCUCh+ } 12, 53 -- Nm7g== } 12, 53 -- X-Gm-Message-State: AOAM530eS76CkVPdHemUW+k4R/iYJ9SQ4uK2AUFcn/iz1sfIZxrRO99g } 12, 53 -- fClgjQTaAJDJgJslUBFXWQ41bX0h } 12, 53 -- X-Google-Smtp-Source: ABdhPJyLM4OyI0Jz4hoqNkzfsYddNyDQTRsD38vkGivBJViSnkszWTTsDIGWixIDMhvqOPS2fq4+3w== } 12, 53 -- X-Received: by 2002:a5d:9e55:: with SMTP id i21mr6351876ioi.130.1591841515147; } 12, 53 -- Wed, 10 Jun 2020 19:11:55 -0700 (PDT) } 12, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:3de2:3386:52e6:cafc]) } 12, 53 -- by smtp.googlemail.com with ESMTPSA id a2sm798598iln.38.2020.06.10.19.11.53 } 12, 53 -- for {microscopy-at-microscopy.com} } 12, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 12, 53 -- Wed, 10 Jun 2020 19:11:54 -0700 (PDT) } 12, 53 -- Subject: viaWWW: Principle of how the SAED pattern is formed } 12, 53 -- References: {202006100901.05A91m3d013813-at-microscopy.com} } 12, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 12, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 12, 53 -- X-Forwarded-Message-Id: {202006100901.05A91m3d013813-at-microscopy.com} } 12, 53 -- Message-ID: {76cdd245-360f-80c5-b1e6-18fb6cf90e1b-at-gmail.com} } 12, 53 -- Date: Wed, 10 Jun 2020 21:11:53 -0500 } 12, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 12, 53 -- Gecko/20100101 Thunderbird/68.9.0 } 12, 53 -- MIME-Version: 1.0 } 12, 53 -- In-Reply-To: {202006100901.05A91m3d013813-at-microscopy.com} } 12, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 12, 53 -- Content-Language: en-US } 12, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
Does anybody know of a service engineer in the northeast who can provide service for an FEI Morgagni TEM?
Please reply to jan.factor-at-purchase.edu
--Many thanks, *Jan Factor* /**/ /*_STAY _ home. _STAY_ safe. _STOP_ the spread. _SAVE_ lives.*/ Jan Robert Factor, Ph.D. Professor of Biology, Chair of Biology Program Purchase College, State University of New York Purchase, NY 10577 jan.factor-at-purchase.edu {mailto:jan.factor-at-purchase.edu}
*/Coral Reef Biology and Ecology Program/ * *January in Roatan, purchase.edu/coralreef {www.purchase.edu/coralreef} *
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Message: Today we received the renewal of our service contract for our 3DHistech p250 slide scanner and I was shocked to see that 2020/2021 is the last year they will offer the service contract on this instrument-eventhough the instrument is only 4 years old right now! I am used to getting at least 10 years of service contracts on confocals and other microscopes, so I was very shocked to see that for this scanner, 5 years is considered "end of life"....I'm just curious, does anyone have experience with the p250 once it's beyond end of life, is it still possible to get parts and service? I'm also interested to hear if anyone knows for how long Zeiss offers a contract on their slide scanner?
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a lot of time has passed without dealing much with TEM and I am afraid I must recognize that my memory is failing on this one question. I want to avoid propylene oxide as a transfer medium to Epon, so I am dehydrating biological samples with acetone and then I make mixes of acetone:Epon until 100% Epon. I see no reason why it wouldn't work but all protocols use PO as intermediate between dehydrating agent and Epon so I feel lonesome with this idea :-) Are there issues when mixing Epon directly with acetone without using PO? I am quite sure that I already did in the past, some 25 years ago, but perhaps it was already a mistake at this time.
From hennjohn6j-at-gmail.com Fri Jun 19 11:58:59 2020 Return-Path: {hennjohn6j-at-gmail.com} Received: from gmail.com (a99.designerforumail.com [157.52.193.99] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 05JGwwGU001934 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 19 Jun 2020 11:58:59 -0500 Message-ID: {8310319C.4896EB30-at-gmail.com}
X-from: jeffb-at-bio.umass.edu
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Email: jeffb-at-bio.umass.edu
Name: Jeffrey Blanchard
Organization: University of Massachusetts Amherst
Title-Subject: [Filtered] Sharing images
Message: We have a collection of 500 TEM images (each ~35Mb) and growing that we would like to further analyze, annotate and share back with colleagues. To date our collaborators have made the images available via FTP and I send them back notes. Not very satisfactory. It seems like even FLICKR and tags would be an improvement.
} From a few searches and reading an older paper (Eliceiri et al. Biological Imaging Software Tools. Nat Methods. 2012) there are 2 platforms Bisque and OMERO that have been/are being used. Bisque has ties with the iPlant collaborative and is now hosted by Cyverse (and available for a local download). However, I am not sure this is still an active project as I had trouble uploading single images. OMERO looks great and plays well with FIJI and other tools, but seems to require a group/institution set up a server.
Any suggestions? Login Host: 162.245.142.25 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
From stepnoah502ybato-at-gmail.com Thu Jun 25 17:11:15 2020 Return-Path: {stepnoah502ybato-at-gmail.com} Received: from gmail.com (a87.designerforumail.com [157.52.193.87] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 05PMBFO0021093 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 25 Jun 2020 17:11:15 -0500 Message-ID: {F19C3E5C.398E8210-at-gmail.com}
X-from: pragnp-at-rpi.edu
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I am trying to understand the diffraction contrast for my in situ TEM experiments on multilayered samples. I request guidance on any accessible software to simulate the Howie Whelan coupled differential equations. All suggestions are welcome! Thanks in advance.
Best, Prachi
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Email: derrick.horne-at-botany.ubc.ca
Name: Derrick Horne
Organization: UBC BioImaging Facility
Title-Subject: [Filtered] Stage Unit Port Error- Hitachi H-7600
Message: Like many other labs, we have been shut down since mid-March due to COVID-19 and have been trying to get ourselves upright again over the last week or so.
In so doing, we have experienced a new to us error on our Hitachi H7600.
6067: Stage unit port error
This is not a vacuum error, and it seems to be some sort of communication port problem, the effect of which is the TEM software freezing, and the microscope console resetting. This only happens AFTER we apply High Voltage, regardless of whether the filament voltage or beam are turned ON, or if the sample rod is in the column. If we do not turn the HV on, then the TEM will sit happily. Sometimes it takes long enough for the error to be generated that we get a beam, and other times it happens during HV ramp-up or as the filament is heating up. Prior to the error, all stage controls work through both the console and software package. After the error, neither the console controls work (console lights go off) nor the software (all functions)
Letting it sit after the error generates a second error; 6354: Comm Unit Reset error. The software will eventually ask to be reset, after which the console will be active again.
The first error can be recreated by unplugging the ribbon cable on port 6507 on the stage controller unit before the HV is applied. I checked continuity (good) and resistance (all the same low value) on the five pins, so Im calling the cable good.
Continuing conversation with our service engineers has not moved us closer to a resolution.
One last point for completeness. The last known thing done to the instrument before we started getting this error was the installation of Chrome Remote Desktop and an antivirus program on the Camera control computer (Win 7) which is separate from the TEM control computer (WinXP). We had those programs removed from the computer, and double checked the log files, which shows the AVP having performed no actions.
Does this problem sound familiar? If so, wed love to hear from you.
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AnnotatePro with Google Drive - does the job and priced just right as freeware.
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 www.partbeamsystech.com www.fibsemproducts.com www.freudlabs.com
On 6/24/2020 8:11 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: jeffb-at-bio.umass.edu } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } jeffb-at-bio.umass.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: jeffb-at-bio.umass.edu } } Name: Jeffrey Blanchard } } Organization: University of Massachusetts Amherst } } Title-Subject: [Filtered] Sharing images } } Message: We have a collection of 500 TEM images (each ~35Mb) and growing that we would like to } further analyze, annotate and share back with colleagues. To date our collaborators have made the } images available via FTP and I send them back notes. Not very satisfactory. It seems like even } FLICKR and tags would be an improvement. } } } From a few searches and reading an older paper (Eliceiri et al. Biological Imaging Software Tools. Nat Methods. 2012) there are 2 platforms Bisque and OMERO that have been/are being used. Bisque has ties with the iPlant collaborative and is now hosted by Cyverse (and available for a local download). However, I am not sure this is still an active project as I had trouble uploading single images. OMERO looks great and plays well with FIJI and other tools, but seems to require a group/institution set up a server. } } Any suggestions? } Login Host: 162.245.142.25 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 11, 53 -- From microscopy.listserver-at-gmail.com Wed Jun 24 19:11:16 2020 } 11, 53 -- Received: from mail-io1-f50.google.com (mail-io1-f50.google.com [209.85.166.50]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 05P0BGDw025768 } 11, 53 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jun 2020 19:11:16 -0500 } 11, 53 -- Received: by mail-io1-f50.google.com with SMTP id f23so4211179iof.6 } 11, 53 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jun 2020 17:16:33 -0700 (PDT) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=ez/GaXamU19+wbcEhB8pnFHl3qeXgYSGQ14yaLRUzhc=; } 11, 53 -- b=R70mIdTvmO82tKIgEHAFiGg6NA9P2k9jydsvkzSCXwS+WBMIx3s5I4zUMKU3MUleWD } 11, 53 -- KyrlXdNmNN5y1+4mXFWiXNWQyo2wYVr9cktrNEWyef20CrUZadBX9G43dpzYC/GvvLTk } 11, 53 -- EDop60kiljGk9lNWAQ0QFn6+p/I8wf4xD4t+YP68np12IZrf874soI0Gk7pq36y11TjE } 11, 53 -- 9ItGgYGivO8+JWB0QDlmHG35DjIALj9mV5b18EkYPvVb9wh1CAwRq822ldkoAu/L5OcC } 11, 53 -- SZ8R79eZ8uCJTRhl6aA7V/mhIz5tnmDuyU95uYIc0DRU/1ubUIXUV9gPnu/wgqjl8s4Q } 11, 53 -- aOrA== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=ez/GaXamU19+wbcEhB8pnFHl3qeXgYSGQ14yaLRUzhc=; } 11, 53 -- b=fkXrYjzVkNrHYsp6FhNhBcQPcLHQe+OrhR1JhACXI+jmZ2ID4RhViOYgVfuyIePbap } 11, 53 -- MDxlbQjD1f/ZPYLz1Bd/69IpoZaVbzkRB+sZK7My1R8l7y1PoeUX7G4JkdwbE6PFR2B7 } 11, 53 -- AE29suvoFh4ljKnglbK7VjoyodpU/ZsrgObLAhTT6EkLnKVxLFqaLquxSiUB5BFY/H5R } 11, 53 -- XDmuOVKFjioaOoHaPEX7hucc4VL5yphHXoKAuNelNojNJFZbZjZLNBNNknSWwGxJgami } 11, 53 -- AMIMkbU+gQBz4p29EeyE+ANHwQ1kORXHXmtGiHOi2MWsoMzuSSIQPsXYoGKDBzvW/0oW } 11, 53 -- +OYQ== } 11, 53 -- X-Gm-Message-State: AOAM532zkYAd8EAVHCGEzcGbTt3S/RGBgL0z3X7TMePHmq7MAbJE9hVL } 11, 53 -- dTUl/lf2ICsMM13gCnD3P5qoD90u } 11, 53 -- X-Google-Smtp-Source: ABdhPJyrjT/yLw/VKiHiFjxrh9+BWclEw+xwK6chMpKd4HGxUgxduSfVU2N6hywvlbQKrluXi7yStQ== } 11, 53 -- X-Received: by 2002:a5e:9705:: with SMTP id w5mr33746538ioj.188.1593044192366; } 11, 53 -- Wed, 24 Jun 2020 17:16:32 -0700 (PDT) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:dc5:1bc4:71b2:6c17]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id n7sm11933194ile.76.2020.06.24.17.16.31 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 11, 53 -- Wed, 24 Jun 2020 17:16:31 -0700 (PDT) } 11, 53 -- Subject: viaWWW: Sharing images } 11, 53 -- References: {202006240229.05O2TkHE029722-at-microscopy.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: {202006240229.05O2TkHE029722-at-microscopy.com} } 11, 53 -- Message-ID: {61bddfce-609d-10ea-8f35-d9eecae14b1b-at-gmail.com} } 11, 53 -- Date: Wed, 24 Jun 2020 19:16:30 -0500 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 11, 53 -- Gecko/20100101 Thunderbird/68.9.0 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: {202006240229.05O2TkHE029722-at-microscopy.com} } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
Box.com has a contract with our university, and sharing images there is great. 🤓
Sent from my iPad
} On Jun 24, 2020, at 7:19 PM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: jeffb-at-bio.umass.edu } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } jeffb-at-bio.umass.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: jeffb-at-bio.umass.edu } } Name: Jeffrey Blanchard } } Organization: University of Massachusetts Amherst } } Title-Subject: [Filtered] Sharing images } } Message: We have a collection of 500 TEM images (each ~35Mb) and growing that we would like to } further analyze, annotate and share back with colleagues. To date our collaborators have made the } images available via FTP and I send them back notes. Not very satisfactory. It seems like even } FLICKR and tags would be an improvement. } } } From a few searches and reading an older paper (Eliceiri et al. Biological Imaging Software Tools. Nat Methods. 2012) there are 2 platforms Bisque and OMERO that have been/are being used. Bisque has ties with the iPlant collaborative and is now hosted by Cyverse (and available for a local download). However, I am not sure this is still an active project as I had trouble uploading single images. OMERO looks great and plays well with FIJI and other tools, but seems to require a group/institution set up a server. } } Any suggestions? } Login Host: 162.245.142.25 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 11, 53 -- From microscopy.listserver-at-gmail.com Wed Jun 24 19:11:16 2020 } 11, 53 -- Received: from mail-io1-f50.google.com (mail-io1-f50.google.com [209.85.166.50]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 05P0BGDw025768 } 11, 53 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jun 2020 19:11:16 -0500 } 11, 53 -- Received: by mail-io1-f50.google.com with SMTP id f23so4211179iof.6 } 11, 53 -- for {microscopy-at-microscopy.com} ; Wed, 24 Jun 2020 17:16:33 -0700 (PDT) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=ez/GaXamU19+wbcEhB8pnFHl3qeXgYSGQ14yaLRUzhc=; } 11, 53 -- b=R70mIdTvmO82tKIgEHAFiGg6NA9P2k9jydsvkzSCXwS+WBMIx3s5I4zUMKU3MUleWD } 11, 53 -- KyrlXdNmNN5y1+4mXFWiXNWQyo2wYVr9cktrNEWyef20CrUZadBX9G43dpzYC/GvvLTk } 11, 53 -- EDop60kiljGk9lNWAQ0QFn6+p/I8wf4xD4t+YP68np12IZrf874soI0Gk7pq36y11TjE } 11, 53 -- 9ItGgYGivO8+JWB0QDlmHG35DjIALj9mV5b18EkYPvVb9wh1CAwRq822ldkoAu/L5OcC } 11, 53 -- SZ8R79eZ8uCJTRhl6aA7V/mhIz5tnmDuyU95uYIc0DRU/1ubUIXUV9gPnu/wgqjl8s4Q } 11, 53 -- aOrA== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=ez/GaXamU19+wbcEhB8pnFHl3qeXgYSGQ14yaLRUzhc=; } 11, 53 -- b=fkXrYjzVkNrHYsp6FhNhBcQPcLHQe+OrhR1JhACXI+jmZ2ID4RhViOYgVfuyIePbap } 11, 53 -- MDxlbQjD1f/ZPYLz1Bd/69IpoZaVbzkRB+sZK7My1R8l7y1PoeUX7G4JkdwbE6PFR2B7 } 11, 53 -- AE29suvoFh4ljKnglbK7VjoyodpU/ZsrgObLAhTT6EkLnKVxLFqaLquxSiUB5BFY/H5R } 11, 53 -- XDmuOVKFjioaOoHaPEX7hucc4VL5yphHXoKAuNelNojNJFZbZjZLNBNNknSWwGxJgami } 11, 53 -- AMIMkbU+gQBz4p29EeyE+ANHwQ1kORXHXmtGiHOi2MWsoMzuSSIQPsXYoGKDBzvW/0oW } 11, 53 -- +OYQ== } 11, 53 -- X-Gm-Message-State: AOAM532zkYAd8EAVHCGEzcGbTt3S/RGBgL0z3X7TMePHmq7MAbJE9hVL } 11, 53 -- dTUl/lf2ICsMM13gCnD3P5qoD90u } 11, 53 -- X-Google-Smtp-Source: ABdhPJyrjT/yLw/VKiHiFjxrh9+BWclEw+xwK6chMpKd4HGxUgxduSfVU2N6hywvlbQKrluXi7yStQ== } 11, 53 -- X-Received: by 2002:a5e:9705:: with SMTP id w5mr33746538ioj.188.1593044192366; } 11, 53 -- Wed, 24 Jun 2020 17:16:32 -0700 (PDT) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:dc5:1bc4:71b2:6c17]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id n7sm11933194ile.76.2020.06.24.17.16.31 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 11, 53 -- Wed, 24 Jun 2020 17:16:31 -0700 (PDT) } 11, 53 -- Subject: viaWWW: Sharing images } 11, 53 -- References: {202006240229.05O2TkHE029722-at-microscopy.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: {202006240229.05O2TkHE029722-at-microscopy.com} } 11, 53 -- Message-ID: {61bddfce-609d-10ea-8f35-d9eecae14b1b-at-gmail.com} } 11, 53 -- Date: Wed, 24 Jun 2020 19:16:30 -0500 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 11, 53 -- Gecko/20100101 Thunderbird/68.9.0 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: {202006240229.05O2TkHE029722-at-microscopy.com} } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
From codyhuey981y-at-gmail.com Mon Jun 29 19:31:36 2020 Return-Path: {codyhuey981y-at-gmail.com} Received: from gmail.com (a82.designerforumail.com [157.52.193.82] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 05U0VaLG018525 for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 29 Jun 2020 19:31:36 -0500 Message-ID: {776B2AAB.15AC0D6D-at-gmail.com}
X-from: joseph.mowery-at-usda.gov
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Email: joseph.mowery-at-usda.gov
Name: Joe Mowery
Organization: USDA ARS
Title-Subject: [Filtered] CMMS: Summer Speaker Series
Message: Hello everyone, On behalf of the Chesapeake Microscopy & Microanalysis Society (CMMS), were pleased to present a Summer Speaker Series, featuring three engaging microscopy talks in July by guest speakers Nestor J.Zaluzec, John Shields and Bernd Zechmann. Youre invited to join us on Zoom next week for our first virtual talk on July 9th at 3pm.
(1) July 9th at 3pm Nestor J. Zaluzec Ph.D. "Hyperspectral Imaging of Soft and Hard Matter in the Analytical Electron Microscope: Current and Future Prospects"
(2) July 14 at 3pm John Shields Ph.D. "Challenges in preparing samples for food sciences"
(3) July 23 at 3pm Bernd Zechmann Ph.D. "Preparation of plant samples for TEM and SEM investigations why small labs struggle to keep up with technological advances"
Please register at https://forms.gle/QYGo9GWASx7X1NX46
There will be a brief question & answer session after each talk. Attendees are encouraged to use their webcams and microphones to encourage discussion. Hope to see you there!
Sincerely, - Joe Mowery
CMMS President-Elect Biologist, Electron & Confocal Microscopy Unit, USDA ARS Login Host: 199.133.37.143 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both r-bleher-at-northwestern.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: r-bleher-at-northwestern.edu
Name: Reiner Bleher
Organization: Northwestern University
Title-Subject: [Filtered] Postdoc position available at Northwestern University
Message: Dear Colleagues,
a Postdoc position is available in the laboratory of Professor Vinayak Dravid at Northwestern University (Evanston campus). The area of interest for this position is Characterizing the Structure and Dynamics of Soft Matter with multimodal Electron Microscopy. Soft Matter here refers to engineered proteins, megamolecules, protein complexes, MOFs and COFs. The candidate will participate in an extensive cross-disciplinary and collaborative project across Chemistry, Biomedical Engineering, and Cell & Molecular Biology experts at Northwestern and the University of Chicago. The research will be conducted in the NUANCE Center (NSF Center for Facility Excellence in the greater Midwest), which houses comprehensive sample preparation and atomic-nanoscale characterization capabilities for biological, soft, hard, and hybrid structures. In this vibrant environment, there is ample opportunity to learn diverse aspects of synthetic biology, simulation and imaging of unconventional molecules/materials. We are looking for a researcher with hands-on experience in electron microscopy, image processing, and data-processing for the reconstruction of sample structures. The position is initially available for two years, with the potential for extension.
Interested in this unique opportunity? Please send your application with an introduction letter, a CV, a research statement (1 page), and contact information for 3 references (name, postal & email address, phone number) in a single PDF to tara.sadera-at-northwestern.edu and v-dravid-at-northwestern.edu.
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X-from: Richard Beanland {contact-at-integrityscientific.com}
Dear Prachi,
towards the end of last year, like you I was searching for some code to do this - and asked on this listserver. Although some people here were very helpful I did not find a package that I could run easily. My main interest is in simulating diffraction contrast images of dislocations.
I managed to find a copy of the 1973 book by Head, Humble & co. (who covered this work quite comprehensively) in a charity shop. This has Fortran source code. However the version of Fortran is so old I could hardly recognise it - and I think comments were seem as a waste of space at the time, so it is difficult to unpick.
A couple of weeks ago I had a little time to spare so I tackled the problem myself from scratch, with the help of the old books. You can find some python code for 2-beam simulation of dislocations at https://github.com/rbeanland/Howie-Whelan_2-beam. This seems to do a reasonable job for dislocations and will produce a simulation in a minute or two for most cases of interest. There is a trick to speed up the calculation by a factor of a hundred or so by using linear combinations of already calculated solutions that I still need to implement, which will mean it should be possible to calculate these images in less than a second on a modern PC.
This is very much a work in progress and I expect to carry on improving and adding to the code (I would welcome anyone else who is interested in doing the same!) As well as speeding the code up the next obvious step is to add stacking faults and multiple dislocations, which would only take a little work. Three(+)-beam calculations would probably be the next goal, to allow weak-beam images to be simulated.
All the best
Richard
On 28/06/2020 15:47, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: pragnp-at-rpi.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MicroscopyListserver/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } pragnp-at-rpi.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: pragnp-at-rpi.edu } } Name: Prachi Pragnya } } Organization: Rensselaer Polytechnic Institute } } Title-Subject: [Filtered] TEM: Howie Whelan equations; accessible software } } Message: Hello, } } I am trying to understand the diffraction contrast for my in situ TEM experiments on multilayered } samples. I request guidance on any accessible software to simulate the Howie Whelan coupled } differential equations. All suggestions are welcome! Thanks in advance. } } Best, } Prachi } } Login Host: 67.242.92.162 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 11, 53 -- From microscopy.listserver-at-gmail.com Sun Jun 28 09:47:08 2020 } 11, 53 -- Received: from mail-io1-f48.google.com (mail-io1-f48.google.com [209.85.166.48]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 05SEl8Cc023713 } 11, 53 -- for {microscopy-at-microscopy.com} ; Sun, 28 Jun 2020 09:47:08 -0500 } 11, 53 -- Received: by mail-io1-f48.google.com with SMTP id k23so14518386iom.10 } 11, 53 -- for {microscopy-at-microscopy.com} ; Sun, 28 Jun 2020 07:52:37 -0700 (PDT) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=/ZKI7ZZQaCD3DVLXz5Y5IzNReIaNd0M6KWKxQGNFdNo=; } 11, 53 -- b=RhMYyckJFKKYt2Br9Jir7orAcnfc8cO9RmHNrWQbZoz0eMhL6tyy4ekRN9lz0PMVUy } 11, 53 -- afcs70rWf8KMA7d60n9iHJ6xjKLh0ylRqERl9gPTScKt+LWaeXTdidAgF3aKpXMbPnAL } 11, 53 -- +R4SYTbRivXfkXodYlHl0lY52STYxSwCdEZ5wGYFNTihVp3S3FNRe+dar2RjvVt96rpI } 11, 53 -- dZk7W459mAzN+6lgqWAE4rrBdZHvGhaRk+ONeBxqz+917E75Lefvx/nx4ABdmnoDMMXP } 11, 53 -- TIzFGp/nI6+Tqu1e55m+wYY5s1PX66GvZcTauff5cesVxFL8UJR3a5BlxTd8XXSWTySF } 11, 53 -- CMwg== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=/ZKI7ZZQaCD3DVLXz5Y5IzNReIaNd0M6KWKxQGNFdNo=; } 11, 53 -- b=SMqe3EGc5VsV7dbKd182rOgFviKREfOmh5Bdr214pgbpfpaxghR78mLWpGOOJvk8d2 } 11, 53 -- HvSJ0jTrNZU65yuAO3rmOANyZaRaJIORO+ikWd8r3eorCJlRtG1HKKDRfUuub2/alksU } 11, 53 -- WEewOi2LWECGAxuozMbyS6/ZsyFsLX8Y9KgUUC93M5rG/zqjmyBa/4RwGn6lYZPW+T/Z } 11, 53 -- 6NTEg/LJNdDOycdnJZ9QFVJNX4JyvBopzazVYRuyVzeBgoDpDxS2V/6Lt9GMHSFGruvU } 11, 53 -- ASRr9MJXQZkzQ454f+vcVn0tq3RP6bGFQ8cO6Jz5iiqj4Z05kR7ryowJcKKdibOqp0Ce } 11, 53 -- +JvQ== } 11, 53 -- X-Gm-Message-State: AOAM530Mtgdr5iePFDnxQxZFftozSAgsATo3M96Nhte4cDRQ3Ee8teZt } 11, 53 -- lsIEUPRgWb8+cAm6RL+gtBTniPrC } 11, 53 -- X-Google-Smtp-Source: ABdhPJymvFAcepVl1XF/6AtzGF/shF9pXkWfpEVhaF6YrbwNauCHOkG4JoXqr+j7BgE63TpRJkqy5A== } 11, 53 -- X-Received: by 2002:a5d:9288:: with SMTP id s8mr12811737iom.42.1593355956635; } 11, 53 -- Sun, 28 Jun 2020 07:52:36 -0700 (PDT) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:1e1:cd7f:4315:c3ca]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id a73sm5287675ill.83.2020.06.28.07.52.35 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 11, 53 -- Sun, 28 Jun 2020 07:52:36 -0700 (PDT) } 11, 53 -- Subject: viaWWW: TEM: Howie Whelan equations; accessible software } 11, 53 -- References: {202006280158.05S1wPuc015445-at-microscopy.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: {202006280158.05S1wPuc015445-at-microscopy.com} } 11, 53 -- Message-ID: {a7669071-7e4d-2254-f321-65a0030f91a7-at-gmail.com} } 11, 53 -- Date: Sun, 28 Jun 2020 09:52:35 -0500 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 11, 53 -- Gecko/20100101 Thunderbird/68.9.0 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: {202006280158.05S1wPuc015445-at-microscopy.com} } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
I was at Ebay and noticed a microchemical test block for sale and it got me wondering. Does Cornell Univ still teach microchemical testing? And who was Shillaber? I recognize Chamot and Mason. I looked at the images and I'm not sure what microcghemical test I could perform with those reagents. Any information you got would be welcome.
Stay calm...Be brave....watch for signs
Frank Karl Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
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Hi List Server,
I am hoping for advice from anyone experienced on collection of blood for TEM analysis of red blood cells. Please advise - is it a good idea to use blood collection tubes with anticoagulants, for example EDTA or LiHeparin. Or is it better not to use these tubes and put the blood straight into fixative.
The blood samples I am about to receive are from reptiles, consequently the volume of blood will be small, only a couple of ml to play with. So any advice on general sample preparation of blood would also be appreciated.
Many thanks in advance,
Sandy Crameri
*Sandy Crameri* Electron Microscopy Australian Centre for Disease Preparedness (ACDP) |CSIRO sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au} |03 5227 5396 |0468 775 317 5 Portarlington Rd East Geelong 3220 |Private Bag 24, Geelong, VIC 3220
| FWD: Re to 'r-bleher-at-northwestern.edu'; Sandra.Crameri-at-csiro.au, and MSA-LISTSERVER: [Microscopy] TEM examination of RBC - which blood collection tube should be [used?] | CAVE: MSA-LISTSERVER ⇒ TEXT FORMATE!
Von: MUSS Wolfgang Dr. phil./PhD (OR i.R, retired) [mailto:wij.muss-at-aon.at] Gesendet: Donnerstag, 2. Juli 2020 15:05 An: 'r-bleher-at-northwestern.edu' {r-bleher-at-northwestern.edu} ; 'Microscopy-at-Microscopy.com' {Microscopy-at-Microscopy.com} Betreff: Re to 'r-bleher-at-northwestern.edu'; Sandra.Crameri-at-csiro.au, and MSA-LISTSERVER: [Microscopy] TEM examination of RBC - which blood collection tube should be [used?]
Hi, dear Sandy,
would you mind to specify more in detail,
i) what the ‚expected‘ volume of „a couple of ml“ would be, and, ii) if that – the whole collected volume of- (peripheral) blood then will be used exclusively for analysis by TEM (= buffy coat preparation) or not.
There might be solutions, but it could turn out to be/ become somehow tricky to do /perform the classical ‚buffy coat‘ – job with small volumina below – say – 4 ml. Thanking you in advance for your kind reply,
Wolfgang
STICK IT OUT! KEEP DISTANCE – SPATIAL, NOT SOCIAL! Keep Distance – Save a Life / perhaps Yours- Stay Safe and Well Stay well and healthy !
===================================================== MUSS Wolfgang Dr. phil. [OR i. R.] / PhD - retired Ignaz-Rieder-Kai 19/6 A-5020 SALZBURG Österreich-AUSTRIA Mobile-Tel.: 0043(0)676 5 369 456 E-mail: wij.muss-at-aon.at E-Mail altern.: womuss-at-gmail.com (will pop up via iPhone-App too)
FRMS, Retired Member of MSA & other (Inter-)National Societies Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS inviting you to join RG (Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html, and join 15+ million researchers, including 63 Nobel Laureates) „ResearchGate is the professional network for scientists and researchers. Over 15 million members from all over the world use it to share, discover, and discuss research. We're guided by our mission to connect the world of science and make research open to all.“
Former Secretary Long standing Member (until March 2018) and (until June2017) Board Member of the SCUR {The Society for Cutaneous Ultrastructure Research} „The Skin Imaging Society“ { www.scur.org }
--------------------------------------------------------------------------------------------------------------------------------------- Von: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Gesendet: Donnerstag, 2. Juli 2020 13:34 An: wij.muss-at-aon.at Betreff: [Microscopy] TEM examination of RBC - which blood collection tube should be [used?]
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Hi List Server,
I am hoping for advice from anyone experienced on collection of blood for TEM analysis of red blood cells. Please advise - is it a good idea to use blood collection tubes with anticoagulants, for example EDTA or LiHeparin. Or is it better not to use these tubes and put the blood straight into fixative.
The blood samples I am about to receive are from reptiles, consequently the volume of blood will be small, only a couple of ml to play with. So any advice on general sample preparation of blood would also be appreciated.
Many thanks in advance,
Sandy Crameri
*Sandy Crameri* Electron Microscopy Australian Centre for Disease Preparedness (ACDP) |CSIRO sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au} |03 5227 5396 |0468 775 317 5 Portarlington Rd East Geelong 3220 | Private Bag 24, Geelong, VIC 3220
Dear Sandra Pleased to greet you, I tell you that I have worked for a while with the blood of patients to diagnose platelet diseases, what I do is obtain the blood in tubes with sodium citrate and it has been wonderful ... If you use tubes with EDTA, I do not think there is a problem ... however, something that has worked for me wonderfully is separating and wash with buffer before fixing, it is important that you do this because the large amount of proteins present in the blood makes the glutaraldehyde to precipitate... Greetings and I hope I can have helped .. If you share your images I will be grateful …
*Biol. Juan Carlos León C.* Investigador en Ciencias Médicas Laboratorio de Microscopia Electrónica Sección Patología Experimental Departamento de Patología Instituto Nacional de Ciencias Médicas y Nutrición S. Z. Ciudad de México. MÉXICO
} Inicio del mensaje reenviado: } } *De: *microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } *Asunto: **[Microscopy] Fwd: TEM examination of RBC - which blood collection tube should be* } *Fecha: *2 de julio de 2020, 6:38:29 GMT-5 } *Para: *jcleonc-at-hotmail.com {mailto:jcleonc-at-hotmail.com} } *Responder a: *microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Crameri, Sandra (AAHL, Geelong ACDP) {Sandra.Crameri-at-csiro.au } {mailto:Sandra.Crameri-at-csiro.au} } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } r-bleher-at-northwestern.edu {mailto:r-bleher-at-northwestern.edu} , Microscopy-at-Microscopy.com } {mailto:Microscopy-at-Microscopy.com} so that all Microscopy Listserver Subscribers } can benefit from our collective wisdom } --------------------------------------------------------------------------- } } } } Hi List Server, } } I am hoping for advice from anyone experienced on collection of blood for TEM analysis of red blood } cells. Please advise - is it a good idea to use blood collection tubes with anticoagulants, for } example EDTA or LiHeparin. Or is it better not to use these tubes and put the blood straight into } fixative. } } The blood samples I am about to receive are from reptiles, consequently the volume of blood will be } small, only a couple of ml to play with. So any advice on general sample preparation of blood would } also be appreciated. } } Many thanks in advance, } } Sandy Crameri } } *Sandy Crameri* } Electron Microscopy } Australian Centre for Disease Preparedness (ACDP) |CSIRO } sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au} {mailto:sandra.crameri-at-csiro.au} |03 5227 } 5396 |0468 775 317 } 5 Portarlington Rd East Geelong 3220 | Private Bag 24, Geelong, VIC 3220 } } } } ==============================Original Headers============================== } 12, 54 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Thu Jul 2 } 06:32:50 2020 } 12, 54 -- Received: from mail-il1-f182.google.com {http://mail-il1-f182.google.com} } (mail-il1-f182.google.com {http://mail-il1-f182.google.com} [209.85.166.182]) } 12, 54 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id } 062BWoPe011195 } 12, 54 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 2 Jul 2020 } 06:32:50 -0500 } 12, 54 -- Received: by mail-il1-f182.google.com {http://mail-il1-f182.google.com} with SMTP id } a11so15651918ilk.0 } 12, 54 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 02 Jul } 2020 04:38:32 -0700 (PDT) } 12, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 54 -- d=gmail.com {http://gmail.com} ; s=20161025; } 12, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 12, 54 -- :in-reply-to:content-language:content-transfer-encoding; } 12, 54 -- bh=vjMtFWkbkzxs2k4Q5KIdDh7Q60t28Q44j7Rzmk1McGA=; } 12, 54 -- b=iKGP4Olq66+NBimys/CCMD1crSDdr1KTnl5kNe4RB9rd+lZoYwnR3avgaPX622yAih } 12, 54 -- JDxOWPEGqgjYBvbPzCItfn2DetASUxHhAJQ1xoBynT8Y72Y/hAQqaaNQnAmcx6fQqtED } 12, 54 -- wwdoaXuf+6SAlZvTjkcQywueZKxbs6S2UhkImCcfFOgjfgt03DI016wrWpFuN28YCQ6+ } 12, 54 -- y6WJb49iAfjgupLGpiGqZgxRi4YonAMO/Z5dXfLIUagx9yocepaR6nUQeOz27gKwBj8r } 12, 54 -- 6BsdI69F7/H3fzpnqH10Zz9zPHjLSYNtrK5/QtnK2cjgDHp/AHPOyAKeU/OCcoSPmUW9 } 12, 54 -- 9J3Q== } 12, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 54 -- d=1e100.net {http://1e100.net} ; s=20161025; } 12, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 12, 54 -- :user-agent:mime-version:in-reply-to:content-language } 12, 54 -- :content-transfer-encoding; } 12, 54 -- bh=vjMtFWkbkzxs2k4Q5KIdDh7Q60t28Q44j7Rzmk1McGA=; } 12, 54 -- b=cZ+uy2Fnf8ztY4N+MgySh07OcXmF7d26zKhqQW2H0o1ryHtRbUH5YIuMRIseacAzXI } 12, 54 -- ZsTvWYbxWF9eaO1AxJKwko7KAWmLBJyNYZA1/ErtKba+hCCe+vC08ZjcaFiFTm7j3DH8 } 12, 54 -- 9MmmQD06uiksj6hfs4+Nne79q0OF1kGrwtfe4K2ETc7HOTDigX4wrGR9Po5YGoLlW3KC } 12, 54 -- 5Tiqfp0OLygEd9iOKdIAaLJ6YMAZh87alhFWmz+ygznaYG/nZHx4hMqgmWyeZJMxFEUA } 12, 54 -- 9Jj2yEH2Au3vUiEPCLVLKdN1Di5qfbIeCptSulLC1d8X2hIZ+2P3vPqiKXMiLjXkSzJf } 12, 54 -- Adbg== } 12, 54 -- X-Gm-Message-State: AOAM533x+4HUyX0nvglsBDau05z+HDVMxPc7VsU7JRHP85K9d/cuo/RX } 12, 54 -- xvsmXsWj+ThR8eiNaY6mmwtcEpdv } 12, 54 -- X-Google-Smtp-Source: } ABdhPJyjOfuzXTI/VZaDmVGrOK6M2srngKcNz/OK9rI0YqitvBu+lAoJjxju3xmbaO50fxGJANOnBg== } 12, 54 -- X-Received: by 2002:a92:25c9:: with SMTP id l192mr12043985ill.135.1593689911538; } 12, 54 -- Thu, 02 Jul 2020 04:38:31 -0700 (PDT) } 12, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } {http://96-65-115-77-static.hfc.comcastbusiness.net} ([2603:300a:f04:7100:d057:9b65:5010:6949]) } 12, 54 -- by smtp.googlemail.com {http://smtp.googlemail.com} with ESMTPSA id } f9sm4788652ilq.9.2020.07.02.04.38.30 } 12, 54 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } } 12, 54 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 12, 54 -- Thu, 02 Jul 2020 04:38:31 -0700 (PDT) } 12, 54 -- Subject: Fwd: TEM examination of RBC - which blood collection tube should be } 12, 54 -- used with anticoagulant or none at all? 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we normally put the blood straight into fixative. Later we normally have to use agar or gelatine to keep the pellet together throughout the processing.
best Leandro
—
Leandro Lemgruber, PhD FRMS
Glasgow Imaging Facility Institute of Infection, Immunity and Inflammation Sir Graeme Davis Building - room B621 University of Glasgow 120 University Place Glasgow, G12 8TA UK
The University of Glasgow, charity number SC004401
The Institute of Infection, Immunity & Inflammation encourages flexible working, so this email may have been sent outside normal working hours. A response is not expected until you return to work.
} On 2 Jul 2020, at 12:34, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Crameri, Sandra (AAHL, Geelong ACDP) {Sandra.Crameri-at-csiro.au } {mailto:Sandra.Crameri-at-csiro.au} } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } r-bleher-at-northwestern.edu {mailto:r-bleher-at-northwestern.edu} , Microscopy-at-Microscopy.com } {mailto:Microscopy-at-Microscopy.com} so that all Microscopy Listserver Subscribers } can benefit from our collective wisdom } --------------------------------------------------------------------------- } } } } Hi List Server, } } I am hoping for advice from anyone experienced on collection of blood for TEM analysis of red blood } cells. Please advise - is it a good idea to use blood collection tubes with anticoagulants, for } example EDTA or LiHeparin. Or is it better not to use these tubes and put the blood straight into } fixative. } } The blood samples I am about to receive are from reptiles, consequently the volume of blood will be } small, only a couple of ml to play with. So any advice on general sample preparation of blood would } also be appreciated. } } Many thanks in advance, } } Sandy Crameri } } *Sandy Crameri* } Electron Microscopy } Australian Centre for Disease Preparedness (ACDP) |CSIRO } sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au} {mailto:sandra.crameri-at-csiro.au} |03 5227 } 5396 |0468 775 317 } 5 Portarlington Rd East Geelong 3220 | Private Bag 24, Geelong, VIC 3220 } } } } ==============================Original Headers============================== } 12, 54 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Thu Jul 2 } 06:32:50 2020 } 12, 54 -- Received: from mail-il1-f182.google.com {http://mail-il1-f182.google.com} } (mail-il1-f182.google.com {http://mail-il1-f182.google.com} [209.85.166.182]) } 12, 54 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id } 062BWoPe011195 } 12, 54 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 2 Jul 2020 } 06:32:50 -0500 } 12, 54 -- Received: by mail-il1-f182.google.com {http://mail-il1-f182.google.com} with SMTP id } a11so15651918ilk.0 } 12, 54 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 02 Jul } 2020 04:38:32 -0700 (PDT) } 12, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 54 -- d=gmail.com {http://gmail.com} ; s=20161025; } 12, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 12, 54 -- :in-reply-to:content-language:content-transfer-encoding; } 12, 54 -- bh=vjMtFWkbkzxs2k4Q5KIdDh7Q60t28Q44j7Rzmk1McGA=; } 12, 54 -- b=iKGP4Olq66+NBimys/CCMD1crSDdr1KTnl5kNe4RB9rd+lZoYwnR3avgaPX622yAih } 12, 54 -- JDxOWPEGqgjYBvbPzCItfn2DetASUxHhAJQ1xoBynT8Y72Y/hAQqaaNQnAmcx6fQqtED } 12, 54 -- wwdoaXuf+6SAlZvTjkcQywueZKxbs6S2UhkImCcfFOgjfgt03DI016wrWpFuN28YCQ6+ } 12, 54 -- y6WJb49iAfjgupLGpiGqZgxRi4YonAMO/Z5dXfLIUagx9yocepaR6nUQeOz27gKwBj8r } 12, 54 -- 6BsdI69F7/H3fzpnqH10Zz9zPHjLSYNtrK5/QtnK2cjgDHp/AHPOyAKeU/OCcoSPmUW9 } 12, 54 -- 9J3Q== } 12, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 54 -- d=1e100.net {http://1e100.net} ; s=20161025; } 12, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 12, 54 -- :user-agent:mime-version:in-reply-to:content-language } 12, 54 -- :content-transfer-encoding; } 12, 54 -- bh=vjMtFWkbkzxs2k4Q5KIdDh7Q60t28Q44j7Rzmk1McGA=; } 12, 54 -- b=cZ+uy2Fnf8ztY4N+MgySh07OcXmF7d26zKhqQW2H0o1ryHtRbUH5YIuMRIseacAzXI } 12, 54 -- ZsTvWYbxWF9eaO1AxJKwko7KAWmLBJyNYZA1/ErtKba+hCCe+vC08ZjcaFiFTm7j3DH8 } 12, 54 -- 9MmmQD06uiksj6hfs4+Nne79q0OF1kGrwtfe4K2ETc7HOTDigX4wrGR9Po5YGoLlW3KC } 12, 54 -- 5Tiqfp0OLygEd9iOKdIAaLJ6YMAZh87alhFWmz+ygznaYG/nZHx4hMqgmWyeZJMxFEUA } 12, 54 -- 9Jj2yEH2Au3vUiEPCLVLKdN1Di5qfbIeCptSulLC1d8X2hIZ+2P3vPqiKXMiLjXkSzJf } 12, 54 -- Adbg== } 12, 54 -- X-Gm-Message-State: AOAM533x+4HUyX0nvglsBDau05z+HDVMxPc7VsU7JRHP85K9d/cuo/RX } 12, 54 -- xvsmXsWj+ThR8eiNaY6mmwtcEpdv } 12, 54 -- X-Google-Smtp-Source: } ABdhPJyjOfuzXTI/VZaDmVGrOK6M2srngKcNz/OK9rI0YqitvBu+lAoJjxju3xmbaO50fxGJANOnBg== } 12, 54 -- X-Received: by 2002:a92:25c9:: with SMTP id l192mr12043985ill.135.1593689911538; } 12, 54 -- Thu, 02 Jul 2020 04:38:31 -0700 (PDT) } 12, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } {http://96-65-115-77-static.hfc.comcastbusiness.net} ([2603:300a:f04:7100:d057:9b65:5010:6949]) } 12, 54 -- by smtp.googlemail.com {http://smtp.googlemail.com} with ESMTPSA id } f9sm4788652ilq.9.2020.07.02.04.38.30 } 12, 54 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } } 12, 54 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 12, 54 -- Thu, 02 Jul 2020 04:38:31 -0700 (PDT) } 12, 54 -- Subject: Fwd: TEM examination of RBC - which blood collection tube should be } 12, 54 -- used with anticoagulant or none at all? 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From slavfree818guuof-at-gmail.com Fri Jul 3 14:34:45 2020 Return-Path: {slavfree818guuof-at-gmail.com} Received: from gmail.com (a95.designerforumail.com [157.52.193.95] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 063JYiJs008494 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 3 Jul 2020 14:34:45 -0500 Message-ID: {9B771D54.25D52C94-at-gmail.com}
X-from: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
I have a Quanta FEG 250 that is now about 10 years old. It is running the older version of software under Windows XP. I have operated a lot of other scopes from JEOL and Hitachi, but not Zeiss. I have found the software quite usable. I have grown particularly fond of their drag over to zoom function. I use it often and try to use it on other scopes until I realize that they are not an FEI and don't support it. If you have the option to get all the Navigation and stage features, please do. We have an FEI Inspect on campus without those features and it is obviously a lessor SEM. Warren
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Friday, July 3, 2020 7:08 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
X-from: Dimitri Scholz {dimitri.scholz-at-ucd.ie}
Hi, has anybody got practical experience with two FEG SEMs: Zeiss Sigma 300 and FEI Quanta 600?
I have to decide between them. Thank you, Dimitri
/Dimitri Scholz, PhD, Dr. Sci. Director of Biological Imaging Conway Institute University College Dublin UCD Belfield, Dublin 4 Ireland Office: +353-1 716 6736 Mobile: +353-87-7961547 Web: http://conway.ucd.ie/coretech/ LinkedIn Profile {https://www.linkedin.com/profile/view?id=AAIAAAVjAg0BCmFc5Rk54thvrW6k0ZirNtCW_qo&trk=nav_responsive_tab_profile_pic} Publications {https://www.researchgate.net/profile/Dimitri_Scholz/publications}
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we normally put the blood straight into fixative. Later we normally have to use agar or gelatine to keep the pellet together throughout the processing.
best Leandro
—
Leandro Lemgruber, PhD FRMS
Glasgow Imaging Facility Institute of Infection, Immunity and Inflammation Sir Graeme Davis Building - room B621 University of Glasgow 120 University Place Glasgow, G12 8TA UK
The University of Glasgow, charity number SC004401
The Institute of Infection, Immunity & Inflammation encourages flexible working, so this email may have been sent outside normal working hours. A response is not expected until you return to work.
} On 2 Jul 2020, at 12:34, microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Crameri, Sandra (AAHL, Geelong ACDP) {Sandra.Crameri-at-csiro.au } {mailto:Sandra.Crameri-at-csiro.au} } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } r-bleher-at-northwestern.edu {mailto:r-bleher-at-northwestern.edu} , Microscopy-at-Microscopy.com } {mailto:Microscopy-at-Microscopy.com} so that all Microscopy Listserver Subscribers } can benefit from our collective wisdom } --------------------------------------------------------------------------- } } } } Hi List Server, } } I am hoping for advice from anyone experienced on collection of blood for TEM analysis of red blood } cells. Please advise - is it a good idea to use blood collection tubes with anticoagulants, for } example EDTA or LiHeparin. Or is it better not to use these tubes and put the blood straight into } fixative. } } The blood samples I am about to receive are from reptiles, consequently the volume of blood will be } small, only a couple of ml to play with. So any advice on general sample preparation of blood would } also be appreciated. } } Many thanks in advance, } } Sandy Crameri } } *Sandy Crameri* } Electron Microscopy } Australian Centre for Disease Preparedness (ACDP) |CSIRO } sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au} {mailto:sandra.crameri-at-csiro.au} |03 5227 } 5396 |0468 775 317 } 5 Portarlington Rd East Geelong 3220 | Private Bag 24, Geelong, VIC 3220 } } } } ==============================Original Headers============================== } 12, 54 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Thu Jul 2 } 06:32:50 2020 } 12, 54 -- Received: from mail-il1-f182.google.com {http://mail-il1-f182.google.com} } (mail-il1-f182.google.com {http://mail-il1-f182.google.com} [209.85.166.182]) } 12, 54 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id } 062BWoPe011195 } 12, 54 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 2 Jul 2020 } 06:32:50 -0500 } 12, 54 -- Received: by mail-il1-f182.google.com {http://mail-il1-f182.google.com} with SMTP id } a11so15651918ilk.0 } 12, 54 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 02 Jul } 2020 04:38:32 -0700 (PDT) } 12, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 54 -- d=gmail.com {http://gmail.com} ; s=20161025; } 12, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 12, 54 -- :in-reply-to:content-language:content-transfer-encoding; } 12, 54 -- bh=vjMtFWkbkzxs2k4Q5KIdDh7Q60t28Q44j7Rzmk1McGA=; } 12, 54 -- b=iKGP4Olq66+NBimys/CCMD1crSDdr1KTnl5kNe4RB9rd+lZoYwnR3avgaPX622yAih } 12, 54 -- JDxOWPEGqgjYBvbPzCItfn2DetASUxHhAJQ1xoBynT8Y72Y/hAQqaaNQnAmcx6fQqtED } 12, 54 -- wwdoaXuf+6SAlZvTjkcQywueZKxbs6S2UhkImCcfFOgjfgt03DI016wrWpFuN28YCQ6+ } 12, 54 -- y6WJb49iAfjgupLGpiGqZgxRi4YonAMO/Z5dXfLIUagx9yocepaR6nUQeOz27gKwBj8r } 12, 54 -- 6BsdI69F7/H3fzpnqH10Zz9zPHjLSYNtrK5/QtnK2cjgDHp/AHPOyAKeU/OCcoSPmUW9 } 12, 54 -- 9J3Q== } 12, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 12, 54 -- d=1e100.net {http://1e100.net} ; s=20161025; } 12, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 12, 54 -- :user-agent:mime-version:in-reply-to:content-language } 12, 54 -- :content-transfer-encoding; } 12, 54 -- bh=vjMtFWkbkzxs2k4Q5KIdDh7Q60t28Q44j7Rzmk1McGA=; } 12, 54 -- b=cZ+uy2Fnf8ztY4N+MgySh07OcXmF7d26zKhqQW2H0o1ryHtRbUH5YIuMRIseacAzXI } 12, 54 -- ZsTvWYbxWF9eaO1AxJKwko7KAWmLBJyNYZA1/ErtKba+hCCe+vC08ZjcaFiFTm7j3DH8 } 12, 54 -- 9MmmQD06uiksj6hfs4+Nne79q0OF1kGrwtfe4K2ETc7HOTDigX4wrGR9Po5YGoLlW3KC } 12, 54 -- 5Tiqfp0OLygEd9iOKdIAaLJ6YMAZh87alhFWmz+ygznaYG/nZHx4hMqgmWyeZJMxFEUA } 12, 54 -- 9Jj2yEH2Au3vUiEPCLVLKdN1Di5qfbIeCptSulLC1d8X2hIZ+2P3vPqiKXMiLjXkSzJf } 12, 54 -- Adbg== } 12, 54 -- X-Gm-Message-State: AOAM533x+4HUyX0nvglsBDau05z+HDVMxPc7VsU7JRHP85K9d/cuo/RX } 12, 54 -- xvsmXsWj+ThR8eiNaY6mmwtcEpdv } 12, 54 -- X-Google-Smtp-Source: } ABdhPJyjOfuzXTI/VZaDmVGrOK6M2srngKcNz/OK9rI0YqitvBu+lAoJjxju3xmbaO50fxGJANOnBg== } 12, 54 -- X-Received: by 2002:a92:25c9:: with SMTP id l192mr12043985ill.135.1593689911538; } 12, 54 -- Thu, 02 Jul 2020 04:38:31 -0700 (PDT) } 12, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } {http://96-65-115-77-static.hfc.comcastbusiness.net} ([2603:300a:f04:7100:d057:9b65:5010:6949]) } 12, 54 -- by smtp.googlemail.com {http://smtp.googlemail.com} with ESMTPSA id } f9sm4788652ilq.9.2020.07.02.04.38.30 } 12, 54 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } } 12, 54 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 12, 54 -- Thu, 02 Jul 2020 04:38:31 -0700 (PDT) } 12, 54 -- Subject: Fwd: TEM examination of RBC - which blood collection tube should be } 12, 54 -- used with anticoagulant or none at all? 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Email: randi.olsen-at-uit.no
Name: Randi Olsen
Organization: Univsersity of Tromso
Title-Subject: [Filtered] Antigen retrieval in MW posessor
Message: Hello to everyone from up north in Norway.
I have a young scientist here looking for a protocol for antigent retrieval in cryostat sections. She has tried a lot, without finding a sucessful portocol, so therefor I would kindly as anyone that might be able to help us out:
Do you have a protocol for antigen retrieval using a Pelco BioWave Pro system?
Best regards, Randi Olsen senior engeneer Advanced microscopy core facility UiT - The arctic University of Tromso Norway
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I think the person who collects the green sea turtle blood I have worked on just puts it into Trump's fixative. I will double-check. Terrestrial reptiles?
Aloha, Tina
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Hi List Server,
I am hoping for advice from anyone experienced on collection of blood for TEM analysis of red blood cells. Please advise - is it a good idea to use blood collection tubes with anticoagulants, for example EDTA or LiHeparin. Or is it better not to use these tubes and put the blood straight into fixative.
The blood samples I am about to receive are from reptiles, consequently the volume of blood will be small, only a couple of ml to play with. So any advice on general sample preparation of blood would also be appreciated.
Many thanks in advance,
Sandy Crameri
*Sandy Crameri* Electron Microscopy Australian Centre for Disease Preparedness (ACDP) |CSIRO sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au {mailto:sandra.crameri-at-csiro.au} } |03 5227 5396 |0468 775 317 5 Portarlington Rd East Geelong 3220 | Private Bag 24, Geelong, VIC 3220
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both anpenn-at-ncsu.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: anpenn-at-ncsu.edu Name: Aubrey Penn
Organization: Microscopy Society of America Student Council
Title-Subject: [Filtered] Preparing for M&M Webinar
Message: Dear Microscopists,
While we regret that the annual Microscopy & Microanalysis conference will not be held in person in Milwaukee in August as originally planned, we are excited to let you know that MSA is planning for a virtual conference August 3-7, and that our X-60 Pre-Meeting Congress for Students, Post-Docs, and Early-Career Professionals will also be held virtually on August 3.
To assist meeting attendees in preparing for the virtual conference, we are hosting a webinar entitled Preparing for M&M at 4 pm Eastern Time on Thursday, July 16. The goal of this webinar is to prepare meeting attendees for what to expect at the virtual conference and to help them get the most out of the experience. To that end, our panelists will answer questions about the layout of the conference, scientific symposia, networking opportunities, vendor tutorials and booths, student programs, and more!
This webinar will present important information for the virtual conference, as well as general opportunities available through the M&M experience. As such, this is a great opportunity to learn more about M&M even if youve attended the conference before.
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Also, please be on the lookout for more information about the webinar and our panelists through email, MSA updates, and our social media platforms.
Please consider joining us on July 16 for this webinar, and we look forward to seeing you at M&M!
Sincerely,
Jackson Spurling Treasurer, MSA Student Council
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Please allow me to express my excitement to be at last able for the first time in my life to attend to the M&M conference! I've never been able to attend to it because of the distance/costs. I hope will be held virtually again in the future! Only problem for europeans is the time lag. Will all sessions be available for replay on demand?
Best regards, Stephane
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Email: anpenn-at-ncsu.edu Name: Aubrey Penn
Organization: Microscopy Society of America Student Council
Title-Subject: [Filtered] Preparing for M&M Webinar
Message: Dear Microscopists,
While we regret that the annual Microscopy & Microanalysis conference will not be held in person in Milwaukee in August as originally planned, we are excited to let you know that MSA is planning for a virtual conference August 3-7, and that our X-60 Pre-Meeting Congress for Students, Post-Docs, and Early-Career Professionals will also be held virtually on August 3.
To assist meeting attendees in preparing for the virtual conference, we are hosting a webinar entitled “Preparing for M&M” at 4 pm Eastern Time on Thursday, July 16. The goal of this webinar is to prepare meeting attendees for what to expect at the virtual conference and to help them get the most out of the experience. To that end, our panelists will answer questions about the layout of the conference, scientific symposia, networking opportunities, vendor tutorials and booths, student programs, and more!
This webinar will present important information for the virtual conference, as well as general opportunities available through the M&M experience. As such, this is a great opportunity to learn more about M&M — even if you’ve attended the conference before.
If you are interested in attending the webinar, please register at this link: https://docs.google.com/forms/d/e/1FAIpQLSeyjz5Ky2EvVvYJYVIDt5mR6kDJjuNXT68Yz3ohAObqcvjrqQ/viewform?usp=sf_link
Also, please be on the lookout for more information about the webinar and our panelists through email, MSA updates, and our social media platforms.
Please consider joining us on July 16 for this webinar, and we look forward to seeing you at M&M!
Sincerely,
Jackson Spurling Treasurer, MSA Student Council
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We, too, are excited about the possibilities opened up by having a virtual M&M meeting! The registration fee for students and postdocs is only $20 For members, $75, and non-members, $150.
All of the platform talks and posters will be available on demand for the entire length of the meeting. At scheduled times the presentations will be streamed, and presenters will be available for live text chats. — Presenters have the option of having their content for an extra month, if they wish.
Some events will be live-streamed, including the plenary session, live chats with exhibitors, and some social gatherings.
The week-at-a-glance calendar for the meeting will be coming out within the next few days, so keep an eye out for that.
See you there!
Sincerely, Esther — Esther Bullitt, Ph.D. President 2020, Microscopy Society of America
} On Jul 8, 2020, at 3:26 AM, nizets2-at-yahoo.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Dear colleagues, } } Please allow me to express my excitement to be at last able for the first time in my life to attend to the M&M conference! } I've never been able to attend to it because of the distance/costs. I hope will be held virtually again in the future! } Only problem for europeans is the time lag. } Will all sessions be available for replay on demand? } } Best regards, } Stephane } } } } } } } On Wednesday, July 8, 2020, 12:47:01 AM GMT+2, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote: } } } } } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: anpenn-at-ncsu.edu } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } anpenn-at-ncsu.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: anpenn-at-ncsu.edu Name: Aubrey Penn } } Organization: Microscopy Society of America Student Council } } Title-Subject: [Filtered] Preparing for M&M Webinar } } Message: Dear Microscopists, } } While we regret that the annual Microscopy & Microanalysis conference will not be held in person in } Milwaukee in August as originally planned, we are excited to let you know that MSA is planning for a } virtual conference August 3-7, and that our X-60 Pre-Meeting Congress for Students, Post-Docs, and } Early-Career Professionals will also be held virtually on August 3. } } To assist meeting attendees in preparing for the virtual conference, we are hosting a webinar } entitled “Preparing for M&M” at 4 pm Eastern Time on Thursday, July 16. The goal of this webinar is } to prepare meeting attendees for what to expect at the virtual conference and to help them get the } most out of the experience. To that end, our panelists will answer questions about the layout of the } conference, scientific symposia, networking opportunities, vendor tutorials and booths, student } programs, and more! } } This webinar will present important information for the virtual conference, as well as general } opportunities available through the M&M experience. As such, this is a great opportunity to learn } more about M&M — even if you’ve attended the conference before. } } If you are interested in attending the webinar, please register at this link: } https://docs.google.com/forms/d/e/1FAIpQLSeyjz5Ky2EvVvYJYVIDt5mR6kDJjuNXT68Yz3ohAObqcvjrqQ/viewform?usp=sf_link } } Also, please be on the lookout for more information about the webinar and our panelists through } email, MSA updates, and our social media platforms. } } Please consider joining us on July 16 for this webinar, and we look forward to seeing you at M&M! } } Sincerely, } } Jackson Spurling } Treasurer, MSA Student Council } } Login Host: 107.13.165.234 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 16, 53 -- From microscopy.listserver-at-gmail.com Tue Jul 7 17:32:38 2020 } 16, 53 -- Received: from mail-io1-f54.google.com (mail-io1-f54.google.com [209.85.166.54]) } 16, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 067MWcfO022896 } 16, 53 -- for {microscopy-at-microscopy.com} ; 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Join us for the webinar series on X-ray Computed Tomography for Materials Science & Life Science to find out how X-ray CT can help your research.
When: July 15, 2020 11:00 AM Pacific Daylight Saving Time
X-from: jpshield-at-uga.edu
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both jpshield-at-uga.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: jpshield-at-uga.edu
Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] SEM filaments
Message: I have two unopened boxes of "standard loop filaments" for "AEI, Cambridge S Series, Novascan, Semco and Nanolab"(EMS Cat# 81020). Our Zeiss 1450 SEM is no longer with us and so we no longer need them.
If you can use the filaments, I will send them (USA shipping only).
Gracias, John Shields Georgia Electron Microscopy UGA Athens, GA
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Hi Randi, Could you provide information on what type of tissue it is? I don't have a lot of personal experience, but one of my users has found that an InstantPot (kitchen pressure cooker) works best for her liver and skin sections. Wishing you peace and good health,
Wendy
-------------------- Wendy C Salmon, M.A. Manager, W.M. Keck Facility for Biological Imaging /Limited availability before 1pm M,W,F due to 2-year-old side-kick/
Whitehead Institute for Biomedical Research 455 Main St., Rm 447 Cambridge, MA 02142 USA e: wsalmon-at-wi.mit.edu {mailto:wsalmon-at-wi.mit.edu} w: microscopy.wi.mit.edu {http://microscopy.wi.mit.edu} she/her/hers {http://microscopy.wi.mit.edu}
On Wed, Jul 8, 2020 at 12:39 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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Title-Subject: [Filtered] Antigen retrieval in MW posessor
Message: Hello to everyone from up north in Norway.
I have a young scientist here looking for a protocol for antigent retrieval in cryostat sections. She has tried a lot, without finding a sucessful portocol, so therefor I would kindly as anyone that might be able to help us out:
Do you have a protocol for antigen retrieval using a Pelco BioWave Pro system?
Best regards, Randi Olsen senior engeneer Advanced microscopy core facility UiT - The arctic University of Tromso Norway
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I'm looking for an instruction manual for a AO Microtome Model 880. This is a manual. Clamp to table microtome. I've been interested in cutting TS of wood but really like the instructions. Does anyone have a e-copy?
Stay calm…Be brave….Watch for signs
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
The virtual M&M 2020 schedule is out now! https://www.microscopy.org/MandM/2020/MM2020_schedule.pdf
The meeting website is: https://www.microscopy.org/MandM/2020/
And to register for 'Preparing for M&M webinar’ presented by the Microscopy Society Student Counci on July 16 at 4, Go to: https://docs.google.com/forms/d/e/1FAIpQLSeyjz5Ky2EvVvYJYVIDt5mR6kDJjuNXT68Yz3ohAObqcvjrqQ/viewform?usp=sf_link
There are a couple of protocols at the TPI website using the Pelco BioWave system. A buffer of choice would be EDTA, although you can try several other buffers depending upon the biomarkers you are using. Make sure you are using fresh charged slides for your sections to avoid lifting or trapping.
Kindest regards, Jerry Jasso, Regional Manager Element Pi jerry-at-elementpi.com {mailto:jerry-at-elementpi.com} (330) 319-4236
On Thu, Jul 9, 2020 at 8:34 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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Hi Randi, Could you provide information on what type of tissue it is? I don't have a lot of personal experience, but one of my users has found that an InstantPot (kitchen pressure cooker) works best for her liver and skin sections. Wishing you peace and good health,
Wendy
-------------------- Wendy C Salmon, M.A. Manager, W.M. Keck Facility for Biological Imaging /Limited availability before 1pm M,W,F due to 2-year-old side-kick/
Whitehead Institute for Biomedical Research 455 Main St., Rm 447 Cambridge, MA 02142 USA e: wsalmon-at-wi.mit.edu {mailto:wsalmon-at-wi.mit.edu} {mailto:wsalmon-at-wi.mit.edu {mailto:wsalmon-at-wi.mit.edu} } w: microscopy.wi.mit.edu {http://microscopy.wi.mit.edu} {http://microscopy.wi.mit.edu} she/her/hers {http://microscopy.wi.mit.edu}
On Wed, Jul 8, 2020 at 12:39 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} {mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } } wrote:
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Title-Subject: [Filtered] Antigen retrieval in MW posessor
Message: Hello to everyone from up north in Norway.
I have a young scientist here looking for a protocol for antigent retrieval in cryostat sections. She has tried a lot, without finding a sucessful portocol, so therefor I would kindly as anyone that might be able to help us out:
Do you have a protocol for antigen retrieval using a Pelco BioWave Pro system?
Best regards, Randi Olsen senior engeneer Advanced microscopy core facility UiT - The arctic University of Tromso Norway
Login Host: 129.242.153.53 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both eric.gautron-at-cnrs-imn.fr, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: eric.gautron-at-cnrs-imn.fr
Name: Eric
Organization: CNRS
Title-Subject: [Filtered] TEM H-9000NAR trouble
Message: Dear all, I have this problem with my old H-9000 NAR : the sample position display indicates E-2 on X, and there is no other indication on Y, Tilt and azimuth. I do not remember what it coresponds to. Any idea to solve that ? Best regards, Eric
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both jpshield-at-uga.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: jpshield-at-uga.edu
Name: John Shields
Organization: University of Georgia
Title-Subject: [Filtered] Filaments gone!
Message: Hi all! The aforementioned SEM filaments have already been spoken for. Thanks! John S Georgia Electron Microscopy
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From 111-at-agrizaturini.com.com Sat Jul 11 22:27:04 2020 Return-Path: {111-at-agrizaturini.com.com} Received: from mail.agrizaturini.com (mail.agrizaturini.com [185.82.217.205] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 06C3R4fM008511; Sat, 11 Jul 2020 22:27:04 -0500 Message-Id: {202007120327.06C3R4fM008511-at-microscopy.com} Received: from [176.32.27.236] (helo=User) by mail.agrizaturini.com with esmtpa (Exim 4.92.3) (envelope-from {111-at-agrizaturini.com} ) id 1juSfl-0005uH-RR; Sat, 11 Jul 2020 23:29:13 -0400 Reply-To: {fernandezfernandez3-at-aol.com}
X-from: nyilmaz-at-mersin.edu.tr
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Email: nyilmaz-at-mersin.edu.tr
Name: sakir necat yilmaz
Organization: University of Mersin
Title-Subject: [Filtered] Megaview III camera problem
Message: Hi All,
We're using a Megaview III camera installed on Jeol JEM 1011 microscope. We've an air leakage problem from o-rings of camera prism bars. Does anybody knows the sizes and/or part number of these o-rings on that bars. Cheers...
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Email: ravi.thakkar369-at-gmail.com
Name: Ravi
Title-Subject: [Filtered] SEM filament blown.
Message: Hi, I am using Hitachi S3500-N SEM, it runs on tungsten filament. The filament is getting blown as I start the HV, it shows 0 current. I lost of three brand new filaments in a row. Can anyone help me how to troubleshoot this?
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We are decommissioning our vintage JEOL 2000FX system and all associated accessories--chiller, UPS, sample holders, and spare parts separate from the instrument itself. The system is in working order though many of the attachments are no longer functional, including the EDS system and STEM scan generator and detectors. Basically, it's useful as a 80-200kV TEM. A Gatan bottom mount Orius camera is attached and functional.
The system will be sent to surplus in 30 days or less. If you're interested in taking the complete system, or just want the holders/spare parts, please contact me ASAP. We cannot cover the expense of moving the system nor shipping of any parts. We also cannot cannibalize the microscope column for parts. Most importantly, since we are a non-profit organization, we can only donate to other non-profits; we cannot sell or give away the system and/or accessories to any for-profit organizations.
You can email or call me directly to discuss.
Thanks, Chris -- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
Hi Ravi We had exactly the same problem with a Jeol JSM5600 and we found that the high tension control board had a transistor blown, we changed it and then was OK. That's all I know and wish you good luck in your diagnosis -then the treatment is simple.. Best regards Yorgos
Tel/fax +30 210 8957677 mobile +30 6945 107477 www.eikonika.netwww.aim.cat *************************************
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Email: ravi.thakkar369-at-gmail.com
Name: Ravi
Title-Subject: [Filtered] SEM filament blown.
Message: Hi, I am using Hitachi S3500-N SEM, it runs on tungsten filament. The filament is getting blown as I start the HV, it shows 0 current. I lost of three brand new filaments in a row. Can anyone help me how to troubleshoot this?
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Rebuilt? (uncommon today, but not unheard-of) Solution - use new filaments, not rebuilts
Pre -centered? Solution - do not trust that they are really pre-centered and make sure and critically center them if necessary.
Make sure that you are setting the correct height in the Wehnelt cap. Do not trust that the height did not change when you took it off. The manual tell you how to properly do this. The depth of the filament in the aperture will determine how much current it takes to cause emission and may cause premature failure.
Important! Critically clean the Wehnelt aperture along with the whole anode cap and cathode cap every time (shiny caps). It is easy to get "in a hurry" and just change the filament. (Also, I know that you know this, but do not handle any of the components with bare fingers.)
Let the chamber pump down extra time after a filament exchange to allow for any potential outgassing in the gun section.
I have had a few bad batches of filaments that have uneven heights and were just poorly made.
It is possible for the current control to be set way too high, or the current control circuit may have failed. While it is a great work horse SEM, the Hitachi S3500-N is getting a little long in the tooth.
Dan Crane (Long time with S3500-N)
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X-from: Regan M {reganhll-at-gmail.com}
Hi, there might be 3 causes.
1. Poor vacuum. Please check ur vacuum gauges before starting the HV. If the gauges are faulty and you open the HV leading to burn out of the filaments. Check the tip using a Light microscope based on how the burn filaments ends appear you could guess the cause of the issue.
2. What is the age of tungsten filaments and from which batch they are. If it so happens that they are all from the same batch it could be that the batch is faulty itself. Test it out with other batch filaments. Supplier must have this data from other users of these filaments.
3. Check your voltage board electronics, There could be a surge which might lead to overload.
Hope it helps, Greetings,
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Message: Hi, I am using Hitachi S3500-N SEM, it runs on tungsten filament. The filament is getting blown as I start the HV, it shows 0 current. I lost of three brand new filaments in a row. Can anyone help me how to troubleshoot this?
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Ravi, I’m inclined to think something is grounding at the grid-cap or anode. Is the grid cap touching the filament tip? How many spacers are going in to the base before screwing on the Wehnalt? Is the anode/grid cap discolored, dirty? How long had your filaments been lasting before this event?
Bil Schneider SEM Lab Manager UW Madison Geosciences 608-333-7874
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Email: ravi.thakkar369-at-gmail.com
Name: Ravi
Title-Subject: [Filtered] SEM filament blown.
Message: Hi, I am using Hitachi S3500-N SEM, it runs on tungsten filament. The filament is getting blown as I start the HV, it shows 0 current. I lost of three brand new filaments in a row. Can anyone help me how to troubleshoot this?
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Can you post a photo of what the burnt out filament looks like somewhere, preferably under an inspection scope? In some cases it can be very helpful for diagnostics.
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Please remember that if you try to post attachements/images to the microscopylistserver they will be rejected. The photo suggested above is fine, but it should be on a public viewing site and just include the URL/link to the image.
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Message: Hi, I am using Hitachi S3500-N SEM, it runs on tungsten filament. The filament is getting blown as I start the HV, it shows 0 current. I lost of three brand new filaments in a row. Can anyone help me how to troubleshoot this?
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From smithdiana157ma-at-gmail.com Thu Jul 16 13:05:54 2020 Return-Path: {smithdiana157ma-at-gmail.com} Received: from gmail.com (a91.designerforumail.com [157.52.193.91] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 06GI5rvM025681 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 16 Jul 2020 13:05:54 -0500 Message-ID: {1FC30DDC.E6EAB99E-at-gmail.com}
X-from: Erico Freitas {ericotadeu-at-ufmg.br}
Hi Ravi,
I would suggest you the same as Dan Crane already said. We have had a similar issue with our W-TEM. A perfect Wehnelt cleaning procedure helped us overcome that. We also vacuum cleaned the gun assembly with a dedicated vacuum cleaner for that with a clean tip (you better not touch the gun assembly with the vacuum cleaner tip, even if that was nicely cleaned up).
Wish you success Regards, Erico
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X-from: Bil Schneider {wfschneider-at-wisc.edu {mailto:wfschneider-at-wisc.edu} }
Ravi, I’m inclined to think something is grounding at the grid-cap or anode. Is the grid cap touching the filament tip? How many spacers are going in to the base before screwing on the Wehnalt? Is the anode/grid cap discolored, dirty? How long had your filaments been lasting before this event?
Bil Schneider SEM Lab Manager UW Madison Geosciences 608-333-7874
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Message: Hi, I am using Hitachi S3500-N SEM, it runs on tungsten filament. The filament is getting blown as I start the HV, it shows 0 current. I lost of three brand new filaments in a row. Can anyone help me how to troubleshoot this?
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Email: becks-at-ncc.edu Name: Steve Beck
Organization: Nassau Community College
Title-Subject: [Filtered] Water sensor
Message: Dear Colleagues,
I have an old Hitachi HS-8 TEM that is having a start up problem. Last year I had to troubleshoot the vacuum power supply module. Now, I cannot get even a low vacuum gauge movement. I noticed the diffusion pump is not heating. There seems to be good flow from my water chiller, however, I am used to hearing a microswitch click when turning the water on and off. Could it be a defective water flow sensor? Are there replacements available for such an old TEM!
Thanks for any suggestions!
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Problem: EDX counts fluctuating down to zero rate for a few seconds Anyone experienced this before ?
The beam current measured at stage with A Keithley meter and illumination from SE are very stable. So I suspect this LN2 EDX is going to need repair. I just had it pumped down to a very good vacuum level hence rid of any ice.
Sylvain
Private owner / hobbyist with an Amray 1850 & Isis EDX
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Email: miketoalson-at-gmail.com Name: Mike Toalson
Organization: Element Pi
Title-Subject: [Filtered] SEM Filament Vibration
Message: Looking for feedback from the EM community. It was recently suggested to me that what appears to be wave like image distortion normally attributed to EMI could actually be induced by vibration of the Tungsten filament that results from incorrect centering or height adjustment in relation to the Wehnelt.
Has anyone looked into this or know if this is possible?
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
Sylvain,
I had a similar problem in a WinDISS EDS system some years ago. The problem was a dead DC/DC converter in the pre-amp at the detector. Easy, cheap fix once diagnosed. I suspect your issue is something like this.
Phil ----------------------------------------- Philip Oshel Imaging Center Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 office (989) 774-7567 lab
________________________________________ X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Friday, July 24, 2020 17:21 To: Oshel, Philip Eugene
Problem: EDX counts fluctuating down to zero rate for a few seconds Anyone experienced this before ?
The beam current measured at stage with A Keithley meter and illumination from SE are very stable. So I suspect this LN2 EDX is going to need repair. I just had it pumped down to a very good vacuum level hence rid of any ice.
Sylvain
Private owner / hobbyist with an Amray 1850 & Isis EDX
Hello Mike, EMI effects can be easily checked when you go to a low Working Distance like 6 mm and set SYNC on scan at 50 or 60 Hz (whatver your power grid has). If effect decreases you have some EMI influence. Using a magnetic fiekd cancellation system like a Spicer 22 will greatly help. Only uf this is ruled out then it might be something as strange as „filament vibration“... Is it dependent on HV value? It might also be a problem with the scan amp or a power supply there.
Best wishes Stefan
------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D-97072 Wuerzburg Germany Tel. +49-931-7848700 Fax +49-931-7848701 Mobile +49-175-7177051
Noise is the usual subject for bickering between users complaining about noise and OEMs unwilling (or being unable) to either resolve the issue with instrument, or convincingly demonstrate that problem originates from the environment. Fortunately there are a few tests you can run on the SEM to point in the direction of the root-cause:
1. As already suggested, change working distance. If the root cause is EMI picked up by the electron beam, then larger WD will have increased noise. Same will happen if the root cause is mechanical vibration of the source. But if source of the noise is in mechanical vibrations picked up by the stage or body of the instrument then magnitude of vibrations wouldn't depend on working distance.
2. Change acceleration voltage by a lot, like 3kV to 30kV (if you can). If the source of noise is EMI picked up by the beam, then lower-energy electrons would be more susceptible to it, and magnitude of the noise with lower acceleration voltage would increase.
3. Go through the range of acceleration voltages. If the source of noise some kind of resonance or cyclic process taking place with the source, or oscillations in high-voltage power supply, then you may see not only magnitude, but also frequency of the noise change.
4. Tap gently on SEM column with the screwdriver. You will see additional noise, but look instead how such tapping affects the original noise that was present. If tapping enhances original noise, or maybe suppresses it for some time, then the likely cause is some kind of mechanical resonance in the column or with the source.
5. Get a stethoscope (or surface-pickup microphone) and listen to sounds of the column. If you hear something appearing when the source is up, and disappearing when the source is off then there is a possibility of root cause being associated with the source operation (oscillations, resonance, etc...).
6. Connect that surface-pickup microphone to audio input of your laptop and run audio spectrum analyzer - frequency and it changes may point to the root cause. For example, if you see frequency matching rotation of your turbo then that could be the original source of noise.
7. Connect spectrum analyzer to the imaging output of your SEM. You will see frequencies associated with line scanning, but look for something related to 50/60Hz or 100/120Hz - would be indicative of poor filtration or a ground loop somewhere. Do this with electron beam blanked, open, and scanning - differences could pin-point which part of the SEM circuitry is affected.
8. I spectrum analyzer is way too exotic animal then try working out frequency of the noise from periodicity of waves and how it changes with change of the scanning frequency.
9. Switching frequency of high-voltage power supply or intermodulation between switching frequencies of its various modules could be the culprit, if decoupling or filtration is inadequate.
10. Change imaging modes of the SEM. If noise is present in one of the modes and not in another - root cause is in SEM electronics.
11. Everything else fails - get engineering help from someone with experience in noise troubleshooting.
Cheers :) Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 www.partbeamsystech.com www.fibsemproducts.com www.freudlabs.com
On 7/26/2020 11:21 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: miketoalson-at-gmail.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } miketoalson-at-gmail.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers } can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: miketoalson-at-gmail.com Name: Mike Toalson } } Organization: Element Pi } } Title-Subject: [Filtered] SEM Filament Vibration } } Message: Looking for feedback from the EM community. It was recently suggested to me that what } appears to be wave like image distortion normally attributed to EMI could actually be induced by } vibration of the Tungsten filament that results from incorrect centering or height adjustment in } relation to the Wehnelt. } } Has anyone looked into this or know if this is possible? } } Login Host: 71.56.132.183 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 9, 53 -- From microscopy.listserver-at-gmail.com Sun Jul 26 10:19:55 2020 } 9, 53 -- Received: from mail-io1-f44.google.com (mail-io1-f44.google.com [209.85.166.44]) } 9, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 06QFJtQE005469 } 9, 53 -- for {microscopy-at-microscopy.com} ; Sun, 26 Jul 2020 10:19:55 -0500 } 9, 53 -- Received: by mail-io1-f44.google.com with SMTP id l17so14460718iok.7 } 9, 53 -- for {microscopy-at-microscopy.com} ; Sun, 26 Jul 2020 08:26:55 -0700 (PDT) } 9, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 53 -- d=gmail.com; s=20161025; } 9, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 9, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 9, 53 -- bh=N7tO81DWnJXEDNfoLrtrL7zyJ2qpka/bi04JcvUce18=; } 9, 53 -- b=Ml3LKKBHFy1nHtfiFAAuSCNyQrNJHxPF+G/9r8Wb6I3Uh4z8WQ3iPZDsELLiLcwdMt } 9, 53 -- 4KjY2HAT9zSZXy9nXOWOZIodhoWMtO6aWwUEKZXbvCEtjo7ViWJ/xNUFgR2TFu8Yt9c1 } 9, 53 -- tqPqnW+dtGeunxXKVgOIc/uHTYL5Y+hlVht5iiIo8pP5aSwSLk/sKNzQNDyn1XJohPQ7 } 9, 53 -- 29rVEtQkZYOWtgdvf5wdXwyRCCCCDNo2aQIT7tPVfLgS70K9EFDKv+qbMDYzCbsFdR2O } 9, 53 -- KbuMEMHO8eJAahJ+l8e27gvEaxASvlUD/OudaqX1mUsO/FaPAX2EFQ93e5gmb5X+i0sc } 9, 53 -- SxTg== } 9, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 53 -- d=1e100.net; s=20161025; } 9, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 9, 53 -- :user-agent:mime-version:in-reply-to:content-language } 9, 53 -- :content-transfer-encoding; } 9, 53 -- bh=N7tO81DWnJXEDNfoLrtrL7zyJ2qpka/bi04JcvUce18=; } 9, 53 -- b=sHShFqwZmrV23uYlpTZB1YUE55YqcQREbdBd4MVnbKifbhPT9c5P5gbPhrnwMAoKMQ } 9, 53 -- Sa2N/I5RwSzw7qk9CvhdQadwNQ4ll1j5lgoxm5Z5nIjqrQ4wj+ciyc5RxdhNqG69FpIr } 9, 53 -- K49loAOte7yuFfOwTNhwvJbLc1ageR6NHGk51iR0hVw950vy05cAW9mK58/EyIYN6fJL } 9, 53 -- /Ooh3AwT8BMCIjgkwRxJWjPJadJ2eR+YEYLXbBh3zDJG7DLSn3HqSzigzqEnZKl/lYTY } 9, 53 -- UCckAc1VN+/qbjx33tAfTGyZJCXRLWBveKsIe+ar9lX8TeSH/vlo4C84Jb6rReUlTJel } 9, 53 -- goEQ== } 9, 53 -- X-Gm-Message-State: AOAM532yv7a1+WJKdvGz7fcEZ50zDaX6oNK5XDWPPlTQvYf0r5q1zm/i } 9, 53 -- P3iXJGInbkJWeLXjaruhHXmUOfGm } 9, 53 -- X-Google-Smtp-Source: ABdhPJxUtwQENYgveDdQVbJfe7kMunC+uPY1EH85g0uqIMIRiNQeiTN1V0khOIVi9pBF8fyBKfS0mA== } 9, 53 -- X-Received: by 2002:a05:6602:220f:: with SMTP id n15mr19955702ion.103.1595777214828; } 9, 53 -- Sun, 26 Jul 2020 08:26:54 -0700 (PDT) } 9, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:446c:64b8:b492:b8e5]) } 9, 53 -- by smtp.googlemail.com with ESMTPSA id c14sm6725765ild.41.2020.07.26.08.26.53 } 9, 53 -- for {microscopy-at-microscopy.com} } 9, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 9, 53 -- Sun, 26 Jul 2020 08:26:54 -0700 (PDT) } 9, 53 -- Subject: viaWWW: SEM Filament Vibration } 9, 53 -- References: {202007252138.06PLcxsg023531-at-microscopy.com} } 9, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 9, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 9, 53 -- X-Forwarded-Message-Id: {202007252138.06PLcxsg023531-at-microscopy.com} } 9, 53 -- Message-ID: {ac84dc49-51ec-2374-4d56-a45da0cdb3ed-at-gmail.com} } 9, 53 -- Date: Sun, 26 Jul 2020 10:26:53 -0500 } 9, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 9, 53 -- Gecko/20100101 Thunderbird/68.10.0 } 9, 53 -- MIME-Version: 1.0 } 9, 53 -- In-Reply-To: {202007252138.06PLcxsg023531-at-microscopy.com} } 9, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 9, 53 -- Content-Language: en-US } 9, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
From normanatlas54-at-gmail.com Sun Jul 26 16:35:55 2020 Return-Path: {normanatlas54-at-gmail.com} Received: from gmail.com (a99.designerforumail.com [157.52.193.99] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 06QLZtMr026464 for {microscopylistserverarchive7-at-microscopy.com} ; Sun, 26 Jul 2020 16:35:55 -0500 Message-ID: {6C8A0E7D.E874874F-at-gmail.com}
X-from: Pat Kelly {probe-at-geotrack.com.au}
We had this problem with our very old but still working EDAX detector recently. Spectra collecting as normal and then suddenly no counts for a few seconds and then back to counting. We tracked it down to the HV supply to the detector. (-750V for our detector) It may be worth checking, make sure you use a high voltage probe with a high input impedence to measure.
Good Luck.
Patrick R Kelly Operations Manager Geotrack International Pty Ltd ABN16 006 821 209 37 Melville Road, Brunswick West, Victoria 3055 Australia Telephone: +613 93801077 Facsimile: +613 93801477 http://www.geotrack.com.au
-----Original Message----- From: microscopy.listserver-at-gmail.com Sent: Saturday, July 25, 2020 7:21 AM To: probe-at-geotrack.com.au
Problem: EDX counts fluctuating down to zero rate for a few seconds Anyone experienced this before ?
The beam current measured at stage with A Keithley meter and illumination from SE are very stable. So I suspect this LN2 EDX is going to need repair. I just had it pumped down to a very good vacuum level hence rid of any ice.
Sylvain
Private owner / hobbyist with an Amray 1850 & Isis EDX
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Email: ali.ibn.rafi-at-gmail.com
Name: Ali Husain
Organization: University of Illinois at Urbana-Champaign Title-Subject: [Filtered] RMC MT6000XL Manual?
Message: Hi all,
I managed to pull an old RMC MT6000XL ultramicrotome from a scrapper and plan to work on it to make is usable. *Does anyone have a copy of the manual for this microtome (or a related model)?* It's so old that I'm even having trouble finding the specifications for it.
Thanks Dr. Ali Husain University of Illinois
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-------- Forwarded Message -------- X-from: Mazurkiewicz, Joseph {MazurkJ-at-amc.edu}
I do. Im on vacation until Aug 10th. Ill scan and send a pdf whe I get back home.
Joe Mazurkiewicz mazurkj-at-amc.edu
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Email: ali.ibn.rafi-at-gmail.com
Name: Ali Husain
Organization: University of Illinois at Urbana-Champaign Title-Subject: [Filtered] RMC MT6000XL Manual?
Message: Hi all,
I managed to pull an old RMC MT6000XL ultramicrotome from a scrapper and plan to work on it to make is usable. *Does anyone have a copy of the manual for this microtome (or a related model)?* It's so old that I'm even having trouble finding the specifications for it.
Thanks Dr. Ali Husain University of Illinois
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From janiceking866isobi-at-gmail.com Thu Jul 30 05:47:37 2020 Return-Path: {janiceking866isobi-at-gmail.com} Received: from gmail.com (a87.designerforumail.com [157.52.193.87] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 06UAlaTY027482 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 30 Jul 2020 05:47:36 -0500 Message-ID: {59B5BA1F.4DD39242-at-gmail.com}
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Email: dale_cornwell-at-yahoo.com
Name: Dale Cornwell
Organization: none
Title-Subject: [Filtered] etec sem filaments
Message: I have a box of ten filaments. anyone interested? also a 545 film holder
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545 film holder. We are showing our age on this one. Remember when one out of the 20 pieces of film would get stuck and then of course one had to clean the developing chemicals out of the holder? With best regards,
Jeff
} On Jul 30, 2020, at 8:50 PM, "microscopy.listserver-at-gmail.com" {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } dale_cornwell-at-yahoo.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers } can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: dale_cornwell-at-yahoo.com } } Name: Dale Cornwell } } Organization: none } } Title-Subject: [Filtered] etec sem filaments } } Message: I have a box of ten filaments. anyone interested? } also a 545 film holder } } Login Host: 108.87.25.11 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 9, 53 -- From microscopy.listserver-at-gmail.com Thu Jul 30 19:34:31 2020 } 9, 53 -- Received: from mail-il1-f175.google.com (mail-il1-f175.google.com [209.85.166.175]) } 9, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 06V0YVGZ018685 } 9, 53 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2020 19:34:31 -0500 } 9, 53 -- Received: by mail-il1-f175.google.com with SMTP id c16so12243547ils.8 } 9, 53 -- for {microscopy-at-microscopy.com} ; Thu, 30 Jul 2020 17:41:46 -0700 (PDT) } 9, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 53 -- d=gmail.com; s=20161025; } 9, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 9, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 9, 53 -- bh=PNLnfVH1FJRyC3Aaupzd6xu92SCRGS6qp0WQ3GXcxAc=; } 9, 53 -- b=iXWpUZ/tuq9FjkZ0r8UW8ih8tZSEpw833H0LsDrvNjpIMRTaO0EwicSZPv0nNJmBI3 } 9, 53 -- X5mwG4N4Tba4hfYLCGVYoOsol20p5OCYx7VeuuA3gSgFI57QguKxz6q/QH92zwSqUl6n } 9, 53 -- csnN9KPCeX4bwqbaYw4L3qNbaDYLm30ZzDg+XHFg4gubT93RUmFMkB+4AU8f77lTW+eK } 9, 53 -- vpwSmY8sz+F4fSQ1FoohC2Fd3IQwVTufo6ilVJAjYSUoXsogUC/I7W+xhUOt7+eHYZIh } 9, 53 -- xxf4mNLG8VujF1j1bnfVl+h/EyGbQXXSLAWSOCsVJR/lOZ6yS6rukjyxgjRPG4PHGufI } 9, 53 -- UEYg== } 9, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 9, 53 -- d=1e100.net; s=20161025; } 9, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 9, 53 -- :user-agent:mime-version:in-reply-to:content-language } 9, 53 -- :content-transfer-encoding; } 9, 53 -- bh=PNLnfVH1FJRyC3Aaupzd6xu92SCRGS6qp0WQ3GXcxAc=; } 9, 53 -- b=qYJl6EN6X5zFL1W41VTBO1KR0MoX3HzjKMrH3gTAUKaiu7Cn55k5SgXbzNvecvh3m6 } 9, 53 -- 5XBXbGknUfLz7Tax8x1Bk3V6hGm43hhLjbvSlS2ysx0z0aeP1dmVC5xxAPKZwhhZ3WJB } 9, 53 -- NNMp5sbnJ/FLOQzmNVfuW8QsGx/LFuyqNtLvfwyoAziicklMO9l9A7w2sTgBN+NZldOM } 9, 53 -- pRqWsV3Z1DGxEjVLt/zVsfkqAZUILwi+K62pdMspoD5eiKO6ui45jTIfA0nnFsmO+UAe } 9, 53 -- 5idCOW3gvrUy0/QWiFWLRtkodS/fob7NXFJLa2Kp+TC8lNk2IMKwxJ7SZ8lbkcbtBjQa } 9, 53 -- lOBQ== } 9, 53 -- X-Gm-Message-State: AOAM5334+qZj/Zdv9nqO+feyzyCcMG6cXBuID7Z4KVsXREGP36WeikdK } 9, 53 -- EPF7qefI/uDcqrU0mXZE2H316nWI } 9, 53 -- X-Google-Smtp-Source: ABdhPJzidfBRfgi8eKwOzzvH1WBypZ4JiCv8GmlV3t6CvKvvgD+U8aetzGRKkZ+Kw2cWPxFizASpLQ== } 9, 53 -- X-Received: by 2002:a05:6e02:e43:: with SMTP id l3mr1183203ilk.11.1596156105459; } 9, 53 -- Thu, 30 Jul 2020 17:41:45 -0700 (PDT) } 9, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:94f9:e304:914c:58d0]) } 9, 53 -- by smtp.googlemail.com with ESMTPSA id j4sm3380976iog.35.2020.07.30.17.41.44 } 9, 53 -- for {microscopy-at-microscopy.com} } 9, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 9, 53 -- Thu, 30 Jul 2020 17:41:44 -0700 (PDT) } 9, 53 -- Subject: viaWWW: ETEC sem filaments } 9, 53 -- References: {202007302256.06UMu5k4010793-at-microscopy.com} } 9, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 9, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 9, 53 -- X-Forwarded-Message-Id: {202007302256.06UMu5k4010793-at-microscopy.com} } 9, 53 -- Message-ID: {6b714227-99d6-b7f7-588e-991d489fbac3-at-gmail.com} } 9, 53 -- Date: Thu, 30 Jul 2020 19:41:44 -0500 } 9, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 9, 53 -- Gecko/20100101 Thunderbird/68.10.0 } 9, 53 -- MIME-Version: 1.0 } 9, 53 -- In-Reply-To: {202007302256.06UMu5k4010793-at-microscopy.com} } 9, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 9, 53 -- Content-Language: en-US } 9, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
From ketcdutj170faazi-at-gmail.com Sun Aug 2 12:29:25 2020 Return-Path: {ketcdutj170faazi-at-gmail.com} Received: from gmail.com (a92.designerforumail.com [157.52.193.92] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 072HTOLS024760 for {microscopylistserverarchive7-at-microscopy.com} ; Sun, 2 Aug 2020 12:29:25 -0500 Message-ID: {8A7B4280.D4A41E9E-at-gmail.com}
X-from: bassimn-at-mcmaster.ca
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Email: bassimn-at-mcmaster.ca Name: Nabil Bassim
Organization: McMaster University
Title-Subject: [Filtered] Postdoc/Research Associate position(s) available
Message: The Bassim research group is looking for up to 4 postdocs to start in the next 6 months. All work would be performed at the Canadian Centre for Electron Microscopy (CCEM) - (web - ccem.mcmaster.ca).
The first position is in TEM of 2-D materials, including sample preparation, EELS mapping of structures, and high resolution STEM imaging. We are seeking a Postdoc or Research Associate who will work on a varied portfolio of projects, including developing novel 2-D van der Waals heterostructures.
The second position is related to in-situ TEM electrochemistry experiments to monitor the microstructural evolution of catalysts.
The 3rd position is a hybrid of optical materials, materials synthesis and low-loss EELS characterization of plasmonic and phononic structures.
The 4th position is in developing Focused Ion Beam techniques, including patterning, serial sectioning and an exploration of ion-sample interactions.
The chosen candidates would also be encouraged to develop their own microscopy techniques using the CCEM infrastructure. The research projects involve strong collaboration with synthesis teams, and proven ability to work in interdisciplinary groups should be demonstrated. Teaching, communication, and presentation skills are part of a successful post-doctoral or research associate position. Day-to-day supervisory tasks with graduate students in the CCEM and within the Bassim research group would be expected.
Requirements for the role include:
MANDATORY
- A Ph.D. in Materials Science or Physics
- A strong track record in publishing TEM-related research
DESIRED
- Interest in mentoring graduate students
- - Experience in aberration-corrected imaging and electron energy loss spectroscopy
- Experience with in-situ techniques
- Capability to perform image simulations and scattering theory
- Sample preparation capabilities using FIB
Pay will range based on level of experience and track record. The appointments are for 1 year, renewable up to 3 years.
Located at McMaster University in Hamilton, Ontario, the CCEM is home to 10 technical staff and hundreds of users with many diverse interests. Hamilton is a lovely city in Southern Ontario, located midway between Toronto and Niagara Falls with a mild (by Canadian standards) winter.
If you are interested, please contact bassimn at mcmaster.ca with a CV and several references.Erase this text and type your question here
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I am going to have to embed skin biopsies in Epon. To give you an idea of the size and form of the sample, imagine a cylinder 6mm in diameter and 6mm deep, then cut this cylinder in 4 pieces along the length. You'll 1/4 of a cylinder with 3mm width and 6mm length. This is quite big to embed but I cannot cut it further, believe me.
I always prepared my samples in Epon with one dimension being at most 1mm, this is the first time that I have to embed such big samples. Fortunately the skin is not that hard so penetration shouldn't be so difficult after all, I just need to adapt the incubation times to be sure that the samples will be entirely perfectly embedded.
Did somebody already deal with samples that size? Which incubation durations would you advise ? The samples have been already kept in fixative (PFA+Glut) for several weeks at 4°C. I want to post-fix in OsO4, dehydrate in acetone and go directly from acetone in Epon. I plan to cure the resin for 3 days at 60-70°C. Thank you in advance!
Dear Stephane, You might find (perhaps!) helpful my general approaches to skin biopsies of that size...(to be cut 'semithin' with a Histo-Diamond Knive edge length 6 mm, from a well known company....Mr. H.Gnaegi!) for correlative TEM Diamond knive -same company - cutting edge 4-5 mm...it is possible to take ultrathin sections e.g. 1.5-2 mm width x 4-5 mm length) in: https://www.researchgate.net/profile/Wolfgang_MUSS/publications:
Restoration of functional skin at an inveterate large ulceration site in a patient with junctional epidermolysis bullosa (EB-J) using autologous, genetically corrected keratinocytes: immunohistochemical, light and electron microscopy findings (https://www.researchgate.net/publication/326045619_Restoration_of_functiona l_skin_at_an_inveterate_large_ulceration_site_in_a_patient_with_junctional_e pidermolysis_bullosa_EB-J_using_autologous_genetically_corrected_keratinocyt es_immunohistochemical_l)
and related Poster https://www.researchgate.net/publication/326045434_Restoration_of_functional _skin_at_an_inveterate_large_ulceration_site_in_a_patient_with_junctional_ep idermolysis_bullosa_EB-J_using_autologous_genetically_corrected_keratinocyte s_immunohistochemical_l
If you want more detailed info on how I processed tissue specs and performed those embeddings and sectioning (semithin & ultrathin) just request. Greetings, best regards
STAY SAFE- Keep spatial not social Distance STAY HEALTY Wolfgang
============================================= MUSS Wolfgang Dr. phil./PhD [OR i. R./retired]
FRMS, Retired Member of MSA & other (Inter-)National Societies
Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG
'Scientific' Profile including 'publications' at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS inviting you to join ResGate. (Sign up - ResearchGate -at- https://www.researchgate.net/signup.SignUp.html, and join 14+ million researchers, including 63 Nobel Laureates
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Hello dear colleagues over the world!
I am going to have to embed skin biopsies in Epon. To give you an idea of the size and form of the sample, imagine a cylinder 6mm in diameter and 6mm deep, then cut this cylinder in 4 pieces along the length. You'll 1/4 of a cylinder with 3mm width and 6mm length. This is quite big to embed but I cannot cut it further, believe me.
I always prepared my samples in Epon with one dimension being at most 1mm, this is the first time that I have to embed such big samples. Fortunately the skin is not that hard so penetration shouldn't be so difficult after all, I just need to adapt the incubation times to be sure that the samples will be entirely perfectly embedded.
Did somebody already deal with samples that size? Which incubation durations would you advise ? The samples have been already kept in fixative (PFA+Glut) for several weeks at 4°C. I want to post-fix in OsO4, dehydrate in acetone and go directly from acetone in Epon. I plan to cure the resin for 3 days at 60-70°C. Thank you in advance!
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both ahk-at-bu.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: ahk-at-bu.edu Name: Anlee Krupp
Organization: Boston University
Title-Subject: [Filtered] EDAX Genesis manual
Message: Hi all,
We replaced our EDAX EDS unit using Genesis software couple years ago, and about to recycle the manual at end of August. Please let me know if you're interested in getting them. They are: 1. EDAX Genesis User's manual 2. Genesis Service/Installation manual 3. TSL (EBSD) operators training course
Thanks.
Anlee Krupp Laboratory Manager Precision Measurement Laboratory Boston University Photonics Center 8 Saint Marys Street, 9th Floor email: ahk-at-bu.edu
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both yuanyuan.2.zhu-at-uconn.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Title-Subject: [Filtered] Postdoc position available on in-situ environmental (S)TEM
Message: The University of Connecticut (UConn), one of the top 20 public universities in the nation, invites applications for a Postdoctoral Research Associate position in the Department of Materials Science and Engineering (MSE) in the School of Engineering and the Institute of Materials Science (IMS). Applicants with a strong background in (scanning) transmission electron microscopy ((S)TEM) and in-situ experiment experiences are encouraged to apply. In this position, you will have the opportunity to engage creative research on investigating structure-property dynamics in advanced functional and structural materials including but not limited to heterogeneous catalysts. You will have the opportunity to work in a state-of-the-art TEM center hosting a probe-corrected Titan Themis STEM, and perform in-situ (gaseous) environmental microscopy at our latest InToEM center (https://today.uconn.edu/school-stories/intoem/). DUTIES AND RESPONSIBILITIES The successful candidate will share a deep commitment to transmission electron microscopy. Working under the supervision of Prof. Yuanyuan Zhu, the candidate will be expected to lead in-situ TEM researches to further the understanding of materials dynamics. The candidate will also contribute to TEM sample preparation, mentor graduate and undergraduate students; write progress reports; interact with research collaborators; prepare and maintain lab equipment and supplies, submit and publish peer reviewed journal papers. MINIMUM QUALIFICATIONS An earned doctorate in Materials Science, Chemistry, Physics or a related discipline. A strong background and extensive research experience in TEM sample preparation and (scanning) transmission electron microscopy characterization. Good written and verbal communication skills. Good research capabilities as evidenced by a record of publication of results in peer-reviewed journals and external presentations at scientific conferences.
PREFERRED QUALIFICATIONS Additionally, a strong background in one or several of these fields is desirable: heterogeneous catalysis, surface science, solid state physics. The candidate is expected to be proficient at three or more of the following techniques including but not limited to: SAED, Nanobeam Electron Diffraction, HRTEM, probe-corrected STEM, EDS mapping, core- (and low-) loss EELS, in-situ heating TEM. Skills and experience in in-situ gas-cell microscopy is highly desired. Strong interpersonal skills including the ability to interact effectively with staffs, students and collaborators. APPOINTMENT TERMS The selected candidate is expected to start immediately or upon mutual agreement. This is a full-time (12-month appointment) position, and is renewable every year. The successful candidates primary academic appointment will be at the UConn main campus in Storrs, CT. Salary will commensurate with qualifications and experience.
TO APPLY Please submit the following: a cover letter; curriculum vitae (with a full list of publication), copies of two representative publications to yuanyuan.2.zhu-at-uconn.edu, with a subject title In-situTEMPostdoc_yourname. Evaluation of applicants will begin immediately and continue until the position is filled. Employment of the successful candidate will be contingent upon the successful completion of a pre-employment criminal background check.
All employees are subject to adherence to the State Code of Ethics, which may be found at http://www.ct.gov/ethics/site/default.asp.
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both g.auchterlonie-at-uq.edu.au, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Organization: Centre for Microscopy & Microanalysis, UQ
Title-Subject: [Filtered] Surface Analysis Scientist - The University of Queensland
Message: Surface Analysis Scientist: Applications close: 18 Aug 2020 (11:00 PM) E. Australia Standard Time
About this opportunity: CMM provides a broad range X-ray diffraction, spectroscopy and imaging services to UQ, including state-of-the-art X-ray Photoelectron Spectroscopy (XPS) with insitu inoperando and correlative capabilities. UQ requires a highly-skilled and experienced Surface Analysis Scientist to both operate and maintain the instrument and to train clients in the design of experiments for, the operation of, and the analysis and interpretation of data from the XPS instrument. In addition, the role will also provide support for the provision of other advance X-ray analysis techniques including XRD, XRF and/or SAXS. The Surface Analysis Scientist needs to be capable of operating effectively within a team environment but also with a high degree of independence. They will need to provide a very high level of expertise in the areas of surface science analysis, and advance X-ray characterisation to support the research groups within UQ, which have a strong focus on the understanding and engineering of material-, chemical- or bio-interfaces. The role will also service the future needs of the next generation of researchers through research into and the development of new and/or enhanced experimental methods, instrumental capabilities and workflows (including data analysis) relevant to these disciplines.
Our ideal candidate: The successful candidate will be a creative, surface scientist and dedicated XPS specialist with a strong interest in the development of new methods in their field of expertise. You will be driven by the desire to understand the molecular structure, chemistry and dynamics of surfaces and how to accurately elucidate these through the latest advances in scientific methods. You will be client focused, enjoy sharing their knowledge and ready to contribute to our team. Although current work rights in Australia would be an advantage, CMM welcomes applications from candidates who do not yet have a work permit. However, any appointment will be conditional to the candidate having secured unconditional work rights in Australia. We value diversity and inclusion, and actively encourage applications from those who bring diversity to the University. Our Diversity and Inclusion webpage contains further information if you require additional support. Accessibility requirements and/or adjustments can be directed to recruitment-at-uq.edu.au.
Please see http://search.jobs.uq.edu.au/caw/en/job/509618/surface-analysis-scientist for full details and to apply.
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both stefano-at-soquelec.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Title-Subject: [Filtered] Glow discharge for TEM grids
Message: Hi, We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like to use it for making TEM grids hydrophilic. We would appreciate any suggestion or comment on how to determine the best parameters for this process.
Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I am wondering if this can cause any differences.
What is an optimal height of the stage or the distance from the bottom surface of the glow discharge insert to the surface of the stage/TEM grids?
In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any comments on how those parameters affect the process? Thank you in advance and best regards, Stefano Rubino
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both stefano-at-soquelec.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Title-Subject: [Filtered] Glow discharge for TEM grids
Message: Hi, We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like to use it for making TEM grids hydrophilic. We would appreciate any suggestion or comment on how to determine the best parameters for this process.
Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I am wondering if this can cause any differences.
What is an optimal height of the stage or the distance from the bottom surface of the glow discharge insert to the surface of the stage/TEM grids?
In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any comments on how those parameters affect the process? Thank you in advance and best regards, Stefano Rubino
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This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both g.auchterlonie-at-uq.edu.au, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Organization: Centre for Microscopy & Microanalysis, UQ
Title-Subject: [Filtered] Surface Analysis Scientist - The University of Queensland
Message: Surface Analysis Scientist: Applications close: 18 Aug 2020 (11:00 PM) E. Australia Standard Time
About this opportunity: CMM provides a broad range X-ray diffraction, spectroscopy and imaging services to UQ, including state-of-the-art X-ray Photoelectron Spectroscopy (XPS) with insitu inoperando and correlative capabilities. UQ requires a highly-skilled and experienced Surface Analysis Scientist to both operate and maintain the instrument and to train clients in the design of experiments for, the operation of, and the analysis and interpretation of data from the XPS instrument. In addition, the role will also provide support for the provision of other advance X-ray analysis techniques including XRD, XRF and/or SAXS. The Surface Analysis Scientist needs to be capable of operating effectively within a team environment but also with a high degree of independence. They will need to provide a very high level of expertise in the areas of surface science analysis, and advance X-ray characterisation to support the research groups within UQ, which have a strong focus on the understanding and engineering of material-, chemical- or bio-interfaces. The role will also service the future needs of the next generation of researchers through research into and the development of new and/or enhanced experimental methods, instrumental capabilities and workflows (including data analysis) relevant to these disciplines.
Our ideal candidate: The successful candidate will be a creative, surface scientist and dedicated XPS specialist with a strong interest in the development of new methods in their field of expertise. You will be driven by the desire to understand the molecular structure, chemistry and dynamics of surfaces and how to accurately elucidate these through the latest advances in scientific methods. You will be client focused, enjoy sharing their knowledge and ready to contribute to our team. Although current work rights in Australia would be an advantage, CMM welcomes applications from candidates who do not yet have a work permit. However, any appointment will be conditional to the candidate having secured unconditional work rights in Australia. We value diversity and inclusion, and actively encourage applications from those who bring diversity to the University. Our Diversity and Inclusion webpage contains further information if you require additional support. Accessibility requirements and/or adjustments can be directed to recruitment-at-uq.edu.au.
Please see http://search.jobs.uq.edu.au/caw/en/job/509618/surface-analysis-scientist for full details and to apply.
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Hello Stefano, We are using an old Polaron sputter-coater for glow-discharge activation of carbon or carbon/formvar coated grids. We have modified it for glow-discharge over 30 years ago and since then we are using it at following settings:
Electrode distance = 40 mm Current = 10 mA (DC) Time = 30 seconds Pressure = 0.1 torr (reduced air atmosphere)
Grids are placed exactly at the border of Crookes dark space on a support made of a mica sheet.
I hope this could help you in setting up your Q150T ES.
Regards Oldrich
P.S. We published a paper on modification of our Polaron sputter-coater in "Microscopy Research and Technique" 30 years ago. If you would like, I could send you a link.
-- Oldřich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group Vídeňská 1083 142 20 Prague 4 Czech Republic
On Thu, 6 Aug 2020 08:30:26 -0500, microscopy.listserver-at-gmail.com wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: stefano-at-soquelec.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both stefano-at-soquelec.com, } Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: stefano-at-soquelec.com Name: Stefano Rubino } } Title-Subject: [Filtered] Glow discharge for TEM grids } } Message: Hi, } We have a Quorum coater (Q150T ES - turbopumped) with the glow } discharge attachment. We would like to use it for making TEM grids } hydrophilic. We would appreciate any suggestion or comment on how to } determine the best parameters for this process. } } Should we use air or Argon as the process gas? Currently, both gas } inlets are hooked to Argon, and I am wondering if this can cause any } differences. } } What is an optimal height of the stage or the distance from the } bottom surface of the glow discharge insert to the surface of the } stage/TEM grids? } } In the default recipe, the time is set at 25 seconds and the current } at 25 mA, does anyone have any comments on how those parameters } affect the process? Thank you in advance and best regards, } Stefano Rubino } } Login Host: 207.216.59.87 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original } Headers============================== 10, 53 -- From } microscopy.listserver-at-gmail.com Thu Aug 6 08:29:09 2020 10, 53 -- } Received: from mail-io1-f50.google.com (mail-io1-f50.google.com } [209.85.166.50]) 10, 53 -- by microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id 076D2T2q027163 10, 53 -- } for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2020 08:02:29 } -0500 10, 53 -- Received: by mail-io1-f50.google.com with SMTP id } g19so37439797ioh.8 10, 53 -- for {microscopy-at-microscopy.com} ; } Thu, 06 Aug 2020 06:10:05 -0700 (PDT) 10, 53 -- DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=gmail.com; } s=20161025; 10, 53 -- } h=subject:references:to:from:message-id:date:user-agent:mime-version } 10, 53 } -- :in-reply-to:content-language:content-transfer-encoding; } 10, 53 -- bh=R/nP2FFC8La5TNRaEnzeK3ssrYmUXGrRDqSvXPAKKEg=; } 10, 53 -- } b=IoTKixwb1M3kO/Yn/NDU1V84wv1fC54m6o8ury+RIvJ4mx5h50KviHMSKYo5pY4rZT } 10, 53 -- } 16SuIfprWPVixGEZMK3SOxuwbTTTiE3A0Qc8uyTYKRvB/9So0X10RnmviW/4RKbxyh7g } 10, 53 -- } gQMBlN5Cv8YJVbdvo/bZ3ptUZW0XKXekU8P5CnLt+Viar/fbGohJVsEV7GaWrlVO2VC3 } 10, 53 } -- /GiYD1a5g4AAlNeVOQmDSn+Uo+demVvoGU8PUpQUB12NBKgN0qS4ZLH5EwNQ3MDj1gZR } 10, 53 -- } DGbp12VgIX8TQdHIji+RbLAPTBJ3aZAGjHJeS2uLwCR1R2OuniE2dQ4ad/ou8H8DMosi } 10, 53 -- FXUg== 10, 53 -- X-Google-DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=1e100.net; } s=20161025; 10, 53 -- } h=x-gm-message-state:subject:references:to:from:message-id:date 10, } 53 -- :user-agent:mime-version:in-reply-to:content-language } 10, 53 -- :content-transfer-encoding; 10, 53 -- } bh=R/nP2FFC8La5TNRaEnzeK3ssrYmUXGrRDqSvXPAKKEg=; 10, 53 -- } b=fuAWHll66v6te925D98D2iq/SSTLdEbJS49ScdVrk3Bo3UXciwk/AmF2bVPCM97Ai2 } 10, 53 -- } RMrJNc1in0MUJpGzWSvGXyaw1g/blGHpuVSB0mzsiGNYKmRyVLeMJjzHV+LwetdTBbYZ } 10, 53 -- } c+hKSLj4qpDBbCPpgAYWQAsiUcPB+Cc7p0jBCfXSrjhhB8+loBQyFk65k1Nsv7k452ho } 10, 53 -- } jHWa9IBwg5HUMTnElvYHify3pdGL8DKLjLjkoYq4RQJFqbKqxX1pu30M8cG36pcQpeYN } 10, 53 -- } pM33AUGo0VY55OQ2TmLVVmczXrEWW+cS9rur1JQrcULiIGxX+327x2awz6UxPJisAh6r } 10, 53 -- gjNQ== 10, 53 -- X-Gm-Message-State: } AOAM531Ihb4ijmYVRzsh+QaQiYrVyY8zsA0lobSPyaYdCqpkU6An46Zl 10, 53 -- } OZKvRTuZiuZS2dBuHIRb60jzkZoT 10, 53 -- X-Google-Smtp-Source: } ABdhPJx4unCnWWC0JamBfZtNMmKHFi6mcSgVeOfqZaf5pm8qFh1OHtvh3wlv6/biQhUj1lnrJFP1kw== } 10, 53 -- X-Received: by 2002:a5d:9752:: with SMTP id } c18mr10048552ioo.10.1596719404681; 10, 53 -- Thu, 06 Aug 2020 } 06:10:04 -0700 (PDT) 10, 53 -- Received: from } 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:f4da:732f:96:55a5]) 10, 53 -- by } smtp.googlemail.com with ESMTPSA id } h12sm3968429ilo.79.2020.08.06.06.10.04 10, 53 -- for } {microscopy-at-microscopy.com} 10, 53 -- (version=TLS1_3 } cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 10, 53 -- Thu, } 06 Aug 2020 06:10:04 -0700 (PDT) 10, 53 -- Subject: viaWWW:Glow } discharge for TEM grids 10, 53 -- References: } {202008052354.075NsANb011544-at-microscopy.com} 10, 53 -- To: } MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 53 -- } From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 10, 53 } -- X-Forwarded-Message-Id: } {202008052354.075NsANb011544-at-microscopy.com} 10, 53 -- Message-ID: } {01464288-9785-dfb8-9b05-a8c373c9da57-at-gmail.com} 10, 53 -- Date: Thu, } 6 Aug 2020 08:10:03 -0500 10, 53 -- User-Agent: Mozilla/5.0 } (Macintosh; Intel Mac OS X 10.14; rv:68.0) 10, 53 -- Gecko/20100101 } Thunderbird/68.11.0 10, 53 -- MIME-Version: 1.0 10, 53 -- } In-Reply-To: {202008052354.075NsANb011544-at-microscopy.com} 10, 53 -- } Content-Type: text/plain; charset=windows-1252; format=flowed 10, 53 } -- Content-Language: en-US 10, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
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==============================Original Headers============================== 15, 26 -- From benada-at-biomed.cas.cz Thu Aug 6 10:09:54 2020 15, 26 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 15, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 076F9rp4023040 15, 26 -- for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2020 10:09:54 -0500 15, 26 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 15, 26 -- (using TLSv1.3 with cipher TLS_AES_256_GCM_SHA384 (256/256 bits) 15, 26 -- key-exchange ECDHE (P-256) server-signature RSA-PSS (2048 bits) server-digest SHA256) 15, 26 -- (No client certificate requested) 15, 26 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id EAFB6D0038D 15, 26 -- for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2020 17:17:25 +0200 (CEST) 15, 26 -- Date: Thu, 6 Aug 2020 17:17:25 +0200 15, 26 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 15, 26 -- To: {microscopy-at-microscopy.com} 15, 26 -- Subject: Re: [Microscopy] viaWWW:Glow discharge for TEM grids 15, 26 -- Message-ID: {20200806171725.1e9ae43e-at-u117ob02} 15, 26 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 15, 26 -- =?UTF-8?B?xIxS?= 15, 26 -- X-Mailer: Claws Mail 3.17.3 (GTK+ 2.24.32; i686-pc-linux-gnu) 15, 26 -- MIME-Version: 1.0 15, 26 -- Content-Type: text/plain; charset=UTF-8 15, 26 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 15, 26 -- X-IoP-CAS-MailScanner-ID: EAFB6D0038D.AD20E 15, 26 -- X-IoP-CAS-MailScanner: Processed 15, 26 -- X-Spam-Status: No 15, 26 -- Content-Transfer-Encoding: 8bit 15, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 076F9rp4023040 ==============================End of - Headers==============================
Quorum support provided me with the following parameters for glow discharge using the glow discharge insert (10262) on a Q150T ES. I mainly use this function to prepare grids for negative staining.
distance: 35 mm below the insert (optimal 30-35 mm, shorter distance takes shorter duration) current: 20 mA, 25-30 mA max duration: 20 s gas: room air (I use a filter on the air inlet)
If you overcook it e.g., excess time or current, too close to the insert, you can burn the carbon and result in cracks. In addition, if you use Ar, you will just etch the coating. Cheers!
-- Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519) The Biology Imaging Facility (https://www.biology.washington.edu/facilities/research/imaging)
On Thu, Aug 6, 2020 at 6:41 AM {microscopy.listserver-at-gmail.com} wrote: } } Email: stefano-at-soquelec.com Name: Stefano Rubino } } Title-Subject: [Filtered] Glow discharge for TEM grids } } Message: Hi, } We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like } to use it for making TEM grids hydrophilic. } We would appreciate any suggestion or comment on how to determine the best parameters for this process. } } Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I } am wondering if this can cause any differences. } } What is an optimal height of the stage or the distance from the bottom surface of the glow discharge } insert to the surface of the stage/TEM grids? } } In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any } comments on how those parameters affect the process? } Thank you in advance and best regards, } Stefano Rubino
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Hi Stefano
Quorum support provided me with the following parameters for glow discharge using the glow discharge insert (10262) on a Q150T ES. I mainly use this function to prepare grids for negative staining.
distance: 35 mm below the insert (optimal 30-35 mm, shorter distance takes shorter duration) current: 20 mA, 25-30 mA max duration: 20 s gas: room air (I use a filter on the air inlet)
If you overcook it e.g., excess time or current, too close to the insert, you can burn the carbon and result in cracks. In addition, if you use Ar, you will just etch the coating. Cheers!
-- Pang (Wai Pang Chan, wpchan-at-uw.edu, PAA A087, 206-685-1519) The Biology Imaging Facility (https://www.biology.washington.edu/facilities/research/imaging)
On Thu, Aug 6, 2020 at 6:41 AM {microscopy.listserver-at-gmail.com} wrote: } } Email: stefano-at-soquelec.com Name: Stefano Rubino } } Title-Subject: [Filtered] Glow discharge for TEM grids } } Message: Hi, } We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like } to use it for making TEM grids hydrophilic. } We would appreciate any suggestion or comment on how to determine the best parameters for this process. } } Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I } am wondering if this can cause any differences. } } What is an optimal height of the stage or the distance from the bottom surface of the glow discharge } insert to the surface of the stage/TEM grids? } } In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any } comments on how those parameters affect the process? } Thank you in advance and best regards, } Stefano Rubino ==============================Original Headers============================== 7, 44 -- From wpchan-at-uw.edu Thu Aug 6 15:48:48 2020 7, 44 -- Received: from mxout23.cac.washington.edu (mxout23.cac.washington.edu [140.142.32.140]) 7, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 076KmmEw014018 7, 44 -- for {Microscopy-at-microscopy.com} ; Thu, 6 Aug 2020 15:48:48 -0500 7, 44 -- Received: from mail-pj1-f72.google.com (mail-pj1-f72.google.com [209.85.216.72]) 7, 44 -- by mxout23.cac.washington.edu (8.14.4+UW20.07/8.14.4+UW19.10) with ESMTP id 076KshH0027798 7, 44 -- (version=TLSv1/SSLv3 cipher=AES128-GCM-SHA256 bits=128 verify=OK) 7, 44 -- for {Microscopy-at-microscopy.com} ; 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7, 44 -- Thu, 06 Aug 2020 13:54:42 -0700 (PDT) 7, 44 -- X-Google-Smtp-Source: ABdhPJw95vIQqbR/XNpD1Ll6eVtgX+SriY4Onm8eqSFeCrz6LZ4n4rEClIYlFLpAW4grslx01Lb0VDe49tNjsvC6arg= 7, 44 -- X-Received: by 2002:a17:902:262:: with SMTP id 89mr8750634plc.31.1596747282469; 7, 44 -- Thu, 06 Aug 2020 13:54:42 -0700 (PDT) 7, 44 -- MIME-Version: 1.0 7, 44 -- References: {202008061309.076D9HAh028235-at-microscopy.com} {CALbJ0i4hkWrMrrJ5eT00KLY_TfpxoHYKzs1GK-Or_0YGyzmxTw-at-mail.gmail.com} 7, 44 -- In-Reply-To: {CALbJ0i4hkWrMrrJ5eT00KLY_TfpxoHYKzs1GK-Or_0YGyzmxTw-at-mail.gmail.com} 7, 44 -- From: Wai Pang Chan {wpchan-at-uw.edu} 7, 44 -- Date: Thu, 6 Aug 2020 13:54:31 -0700 7, 44 -- Message-ID: {CALbJ0i6k0W=jDe-SkTn+vQmapDTCSruA52G42FxV+WfFR0zJ-w-at-mail.gmail.com} 7, 44 -- Subject: [Filtered] Fwd: [Microscopy] viaWWW:Glow discharge for TEM grids 7, 44 -- To: Microscopy-at-microscopy.com 7, 44 -- Content-Type: text/plain; charset="UTF-8" 7, 44 -- X-PMX-Version: 6.4.9.2830568, Antispam-Engine: 2.7.2.2107409, Antispam-Data: 2020.8.6.204517, AntiVirus-Engine: 5.74.0, AntiVirus-Data: 2020.5.13.5740002 7, 44 -- X-PMX-Server: mxout23.cac.washington.edu 7, 44 -- X-Sophos-SenderHistory: ip=209.85.216.72,fs=215620,da=73197153,mc=4835,sc=3,hc=4832,sp=0,fso=22992903,re=48,sd=0,hd=30 7, 44 -- X-Uwash-Spam: Gauge=X, Probability=10%, Report= 7, 44 -- TO_IN_SUBJECT 0.5, HTML_00_01 0.05, HTML_00_10 0.05, BODYTEXTP_SIZE_3000_LESS 0, BODY_SIZE_1700_1799 0, BODY_SIZE_2000_LESS 0, BODY_SIZE_5000_LESS 0, BODY_SIZE_7000_LESS 0, CT_TEXT_PLAIN_UTF8_CAPS 0, DATE_TZ_NA 0, DQ_S_H 0, FROM_EDU_TLD 0, FROM_NAME_PHRASE 0, IN_REP_TO 0, KNOWN_MTA_TFX 0, LEGITIMATE_SIGNS 0, MSG_THREAD 0, REFERENCES 0, SENDER_NO_AUTH 0, SINGLE_URI_IN_BODY 0, SPF_PASS 0, SXL_IP_TFX_WM 0, URI_WITH_PATH_ONLY 0, WEBMAIL_SOURCE 0, __ANY_URI 0, __BODY_NO_MAILTO 0, __BODY_VOICEMAIL 0, __BOUNCE_NDR_SUBJ_EXEMPT 0, __CP_URI_IN_BODY 0, __CT 0, __CT_TEXT_PLAIN 0, __DQ_IP_FSO_LARGE 0, __DQ_NEG_HEUR 0, __DQ_NEG_IP 0, __DQ_S_HIST_1 0, __DQ_S_HIST_2 0, __DQ_S_IP_MC_100_P 0, __DQ_S_IP_MC_10_P 0, __DQ_S_IP_MC_1K_P 0, __DQ_S_IP_MC_1_P 0, __DQ_S_IP_MC_5_P 0, __DQ_S_IP_SC_1_P 0, __FORWARDED_MSG 0, __FRAUD_BODY_WEBMAIL 0, __FRAUD_CONTACT_NAME 0, __FRAUD_WEBMAIL 0, __FUR_RDNS_GMAIL 0, 7, 44 -- __HAS_FROM 0, __HAS_MSGID 0, __HAS_REFERENCES 0, __HELO_GMAIL 0, __HTTPS_URI 0, __IN_REP_TO 0, __MAIL_CHAIN 0, __MIME_TEXT_ONLY 0, __MIME_TEXT_P 0, __MIME_TEXT_P1 0, __MIME_VERSION 0, __MSGID_DOMAIN_NOT_IN_HDRS 0, __NO_HTML_TAG_RAW 0, __PHISH_SPEAR_STRUCTURE_1 0, __RDNS_WEBMAIL 0, __REFERENCES 0, __SANE_MSGID 0, __SINGLE_URI_TEXT 0, __STOCK_PHRASE_24 0, __SUBJ_ALPHA_END 0, __SUBJ_ALPHA_NEGATE 0, __SUBJ_FORWARD 0, __TO_IN_SUBJECT2 0, __TO_MALFORMED_2 0, __TO_NO_NAME 0, __URI_IN_BODY 0, __URI_MAILTO 0, __URI_NOT_IMG 0, __URI_NS , __URI_WITH_PATH 0, __X_GOOGLE_DKIM_SIGNATURE 0, __YOUTUBE_RCVD 0 ==============================End of - Headers==============================
Hello Stefano, We are using an old Polaron sputter-coater for glow-discharge activation of carbon or carbon/formvar coated grids. We have modified it for glow-discharge over 30 years ago and since then we are using it at following settings:
Electrode distance = 40 mm Current = 10 mA (DC) Time = 30 seconds Pressure = 0.1 torr (reduced air atmosphere)
Grids are placed exactly at the border of Crookes dark space on a support made of a mica sheet.
I hope this could help you in setting up your Q150T ES.
Regards Oldrich
P.S. We published a paper on modification of our Polaron sputter-coater in "Microscopy Research and Technique" 30 years ago. If you would like, I could send you a link.
-- Oldřich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group Vídeňská 1083 142 20 Prague 4 Czech Republic
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We would like to use it for making TEM grids } hydrophilic. We would appreciate any suggestion or comment on how to } determine the best parameters for this process. } } Should we use air or Argon as the process gas? Currently, both gas } inlets are hooked to Argon, and I am wondering if this can cause any } differences. } } What is an optimal height of the stage or the distance from the } bottom surface of the glow discharge insert to the surface of the } stage/TEM grids? } } In the default recipe, the time is set at 25 seconds and the current } at 25 mA, does anyone have any comments on how those parameters } affect the process? Thank you in advance and best regards, } Stefano Rubino } } Login Host: 207.216.59.87 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original } Headers============================== 10, 53 -- From } microscopy.listserver-at-gmail.com Thu Aug 6 08:29:09 2020 10, 53 -- } Received: from mail-io1-f50.google.com (mail-io1-f50.google.com } [209.85.166.50]) 10, 53 -- by microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id 076D2T2q027163 10, 53 -- } for {microscopy-at-microscopy.com} ; Thu, 6 Aug 2020 08:02:29 } -0500 10, 53 -- Received: by mail-io1-f50.google.com with SMTP id } g19so37439797ioh.8 10, 53 -- for {microscopy-at-microscopy.com} ; } Thu, 06 Aug 2020 06:10:05 -0700 (PDT) 10, 53 -- DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=gmail.com; } s=20161025; 10, 53 -- } h=subject:references:to:from:message-id:date:user-agent:mime-version } 10, 53 } -- :in-reply-to:content-language:content-transfer-encoding; } 10, 53 -- bh=R/nP2FFC8La5TNRaEnzeK3ssrYmUXGrRDqSvXPAKKEg=; } 10, 53 -- } b=IoTKixwb1M3kO/Yn/NDU1V84wv1fC54m6o8ury+RIvJ4mx5h50KviHMSKYo5pY4rZT } 10, 53 -- } 16SuIfprWPVixGEZMK3SOxuwbTTTiE3A0Qc8uyTYKRvB/9So0X10RnmviW/4RKbxyh7g } 10, 53 -- } gQMBlN5Cv8YJVbdvo/bZ3ptUZW0XKXekU8P5CnLt+Viar/fbGohJVsEV7GaWrlVO2VC3 } 10, 53 } -- /GiYD1a5g4AAlNeVOQmDSn+Uo+demVvoGU8PUpQUB12NBKgN0qS4ZLH5EwNQ3MDj1gZR } 10, 53 -- } DGbp12VgIX8TQdHIji+RbLAPTBJ3aZAGjHJeS2uLwCR1R2OuniE2dQ4ad/ou8H8DMosi } 10, 53 -- FXUg== 10, 53 -- X-Google-DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 10, 53 -- d=1e100.net; } s=20161025; 10, 53 -- } h=x-gm-message-state:subject:references:to:from:message-id:date 10, } 53 -- :user-agent:mime-version:in-reply-to:content-language } 10, 53 -- :content-transfer-encoding; 10, 53 -- } bh=R/nP2FFC8La5TNRaEnzeK3ssrYmUXGrRDqSvXPAKKEg=; 10, 53 -- } b=fuAWHll66v6te925D98D2iq/SSTLdEbJS49ScdVrk3Bo3UXciwk/AmF2bVPCM97Ai2 } 10, 53 -- } RMrJNc1in0MUJpGzWSvGXyaw1g/blGHpuVSB0mzsiGNYKmRyVLeMJjzHV+LwetdTBbYZ } 10, 53 -- } c+hKSLj4qpDBbCPpgAYWQAsiUcPB+Cc7p0jBCfXSrjhhB8+loBQyFk65k1Nsv7k452ho } 10, 53 -- } jHWa9IBwg5HUMTnElvYHify3pdGL8DKLjLjkoYq4RQJFqbKqxX1pu30M8cG36pcQpeYN } 10, 53 -- } pM33AUGo0VY55OQ2TmLVVmczXrEWW+cS9rur1JQrcULiIGxX+327x2awz6UxPJisAh6r } 10, 53 -- gjNQ== 10, 53 -- X-Gm-Message-State: } AOAM531Ihb4ijmYVRzsh+QaQiYrVyY8zsA0lobSPyaYdCqpkU6An46Zl 10, 53 -- } OZKvRTuZiuZS2dBuHIRb60jzkZoT 10, 53 -- X-Google-Smtp-Source: } ABdhPJx4unCnWWC0JamBfZtNMmKHFi6mcSgVeOfqZaf5pm8qFh1OHtvh3wlv6/biQhUj1lnrJFP1kw== } 10, 53 -- X-Received: by 2002:a5d:9752:: with SMTP id } c18mr10048552ioo.10.1596719404681; 10, 53 -- Thu, 06 Aug 2020 } 06:10:04 -0700 (PDT) 10, 53 -- Received: from } 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:f4da:732f:96:55a5]) 10, 53 -- by } smtp.googlemail.com with ESMTPSA id } h12sm3968429ilo.79.2020.08.06.06.10.04 10, 53 -- for } {microscopy-at-microscopy.com} 10, 53 -- (version=TLS1_3 } cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 10, 53 -- Thu, } 06 Aug 2020 06:10:04 -0700 (PDT) 10, 53 -- Subject: viaWWW:Glow } discharge for TEM grids 10, 53 -- References: } {202008052354.075NsANb011544-at-microscopy.com} 10, 53 -- To: } MicroscopyListServer-Forward {microscopy-at-microscopy.com} 10, 53 -- } From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 10, 53 } -- X-Forwarded-Message-Id: } {202008052354.075NsANb011544-at-microscopy.com} 10, 53 -- Message-ID: } {01464288-9785-dfb8-9b05-a8c373c9da57-at-gmail.com} 10, 53 -- Date: Thu, } 6 Aug 2020 08:10:03 -0500 10, 53 -- User-Agent: Mozilla/5.0 } (Macintosh; Intel Mac OS X 10.14; rv:68.0) 10, 53 -- Gecko/20100101 } Thunderbird/68.11.0 10, 53 -- MIME-Version: 1.0 10, 53 -- } In-Reply-To: {202008052354.075NsANb011544-at-microscopy.com} 10, 53 -- } Content-Type: text/plain; charset=windows-1252; format=flowed 10, 53 } -- Content-Language: en-US 10, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
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Stefano, I don't process grids, but I can tell you what is going on with your glow discharge. To make the surface hydrophilic, you are cleaning the hydrocarbons from the surface. You need oxygen to do that well, which you can get from air. Argon can give you some cleaning, but it is not the best process gas. If you were to use pure oxygen, your pumps have to be rated for oxygen. Air or a mixture of Ar-25%O2 is safe for any mechanical pump that uses a hydrocarbon based oil. What is cleaning the grids is activated oxygen molecules which is causing the hydrocarbons to break down and H2O and CO2 to form, which is pumped away. Plasma cleaners do the same thing. The difference between plasma cleaning and glow discharge is the energy of the species that strike the surface. In an RF plasma cleaner, the energy is relatively low. In a DC glow discharge, the energy is higher and it can warm the sample. With copper grids, who cares.
With respect to your distance, I don't know that system. However, as long as the grids are in the plasma, they won't take long to clean. Plasma cleaning is usually 5 minutes or less. I would suspect that glow discharge takes about the same amount of time or less. If you want to experiment with your system, take a piece of material such as copper tape, silicon wafer, or perhaps even a glass slide (I don't know whether that will charge in a DC plasma.) . Put a drop of distilled water on the surface and you will see that it beads up on the surface. Because of the hydrocarbons on the surface, the surface is hydrophobic. It has a high contact angle. After processing, a water droplet will wet out the surface and spread. That is hydrophilic and the contact angle is low. To test how long it takes for a given distance in your coater, use that test surface and see how long it takes to process to give a hydrophilic surface. So for your process parameters, just try them on that test surface. If it doesn't work, do it multiple times until it does and then go from there.
-Scott Walck
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, August 6, 2020 9:35 AM To: s.walck-at-comcast.net
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Title-Subject: [Filtered] Glow discharge for TEM grids
Message: Hi, We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like to use it for making TEM grids hydrophilic. We would appreciate any suggestion or comment on how to determine the best parameters for this process.
Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I am wondering if this can cause any differences.
What is an optimal height of the stage or the distance from the bottom surface of the glow discharge insert to the surface of the stage/TEM grids?
In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any comments on how those parameters affect the process? Thank you in advance and best regards, Stefano Rubino
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X-from: Michel Ribardire {m.ribardiere-at-jeol.fr}
In old time we use an air leak to make plasma and only Rotary Pump to make vacuum to make hydrophilic spcimens. The spcimen was set in place of the mtal deposit target. Michel
Envoy de mon Galaxy A5 2017 Orange
-------- Message d'origine -------- De : microscopy.listserver-at-gmail.com Date : 06/08/2020 15:41 (GMT+01:00) : Michel Ribardire {m.ribardiere-at-jeol.fr} Objet : [Microscopy] viaWWW:Glow discharge for TEM grids
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Title-Subject: [Filtered] Glow discharge for TEM grids
Message: Hi, We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like to use it for making TEM grids hydrophilic. We would appreciate any suggestion or comment on how to determine the best parameters for this process.
Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I am wondering if this can cause any differences.
What is an optimal height of the stage or the distance from the bottom surface of the glow discharge insert to the surface of the stage/TEM grids?
In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any comments on how those parameters affect the process? Thank you in advance and best regards, Stefano Rubino
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From wandaver2ofacx-at-gmail.com Fri Aug 7 05:58:12 2020 Return-Path: {wandaver2ofacx-at-gmail.com} Received: from gmail.com (a110.designerforumail.com [157.52.193.110] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 077AwB2L031001 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 7 Aug 2020 05:58:11 -0500 Message-ID: {C97D6E3B.A0E3C7BE-at-gmail.com}
} On Aug 6, 2020, at 6:34 AM, microscopy.listserver-at-gmail.com wrote: } } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } stefano-at-soquelec.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: stefano-at-soquelec.com Name: Stefano Rubino } } Title-Subject: [Filtered] Glow discharge for TEM grids } } Message: Hi, } We have a Quorum coater (Q150T ES - turbopumped) with the glow discharge attachment. We would like } to use it for making TEM grids hydrophilic. } We would appreciate any suggestion or comment on how to determine the best parameters for this process. } } Should we use air or Argon as the process gas? Currently, both gas inlets are hooked to Argon, and I } am wondering if this can cause any differences. } } What is an optimal height of the stage or the distance from the bottom surface of the glow discharge } insert to the surface of the stage/TEM grids? } } In the default recipe, the time is set at 25 seconds and the current at 25 mA, does anyone have any } comments on how those parameters affect the process? } Thank you in advance and best regards, } Stefano Rubino
Hi Stefano, Oxygen in the plasma interacts with the grid surface to make it hydrophilic, so make sure there is enough in the chamber during discharge. As to your other two questions, I would start with the default and check the grids; if a 3 ul droplet spreads evenly and quickly when applied to the grid, it is sufficiently hydrophilic, if not, increase the time until success. Yours, Bill
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Message: I have some beetle wing covers (elytra) to embed and section for TEM. Anyone have success with this? A typical embedment in EMBed812 (Epon) with a medium hardness is not holding the wing in the section. I'm thinking either a harder formulation, or a different resin. I don't believe fixing it like tissue would make a difference, however I'm not an expert in insect cuticle preparation and sectioning.
Thanks! John Shields Georgia Electron Microscopy
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From ronasyre474bogyv-at-gmail.com Fri Aug 14 05:09:25 2020 Return-Path: {ronasyre474bogyv-at-gmail.com} Received: from gmail.com (a97.designerforumail.com [157.52.193.97] (may be forged)) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 07EA9PpZ020166 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 14 Aug 2020 05:09:25 -0500 Message-ID: {E86CF1E3.96009987-at-gmail.com}
X-from: Paula Keene Pierce {paula-at-excaliburpathology.com}
Hi,
insect exoskeleton is mainly made of chitin.
To soften the chitin, after fixation, place the specimens in a liquid such as Lysol or Simple Green Clean Finish that contains alkyl dimethylbenzylammonium chlorides for 20 min. to 1 hour.
No kidding! I use it for drosophila eyes in paraffin sections.
This works in the same way it kills bacteria, fungus, viruses...
Paula Keene Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Drive Norman, OK 73069 PH 405-759-3953 http://www.excaliburpathology.com
A sharp knife is nothing without a sharp eye. - Klingon Proverb
On Thursday, August 13, 2020, 12:52:08 PM CDT, {microscopy.listserver-at-gmail.com} wrote:
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Email: jpshield-at-uga.edu {mailto:jpshield-at-uga.edu} Name: John Shields
Message: I have some beetle wing covers (elytra) to embed and section for TEM. Anyone have success with this? A typical embedment in EMBed812 (Epon) with a medium hardness is not holding the wing in the section. I'm thinking either a harder formulation, or a different resin. I don't believe fixing it like tissue would make a difference, however I'm not an expert in insect cuticle preparation and sectioning.
Thanks! John Shields Georgia Electron Microscopy
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X-from: Calomeni, Edward {Edward.Calomeni-at-osumc.edu}
John, This sounds like a Dear Abby question. I imagine an answer would likely entail the careful use of a Ginsu Knife. Sorry, cannot be of much further help with insect wings.
Congratulates on winning the Morton Maser award.
Ed Edward P. Calomeni Director EM Lab - Pathology The Ohio State University M018 Starling Loving Hall 320 W. 10th Ave. Columbus, OH 43210 614-293-5580 (office) 614-293-8806 (lab) edward.calomeni-at-osumc.edu
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, August 13, 2020 8:58 AM To: Calomeni, Edward {Edward.Calomeni-at-osumc.edu}
X-from: Mowery, Joseph {joseph.mowery-at-usda.gov}
Hi John,
Adding 1% Z6040 to the ethanol during dehydration functions as a coupling agent to bind the resin to the cuticle, and I can confirm it works on insect wings.
Explained in more detail in my recent M&M2020 article: "Utilization of Z-6040 Organosilane as a Coupling Agent to Improve the Adhesion of Epoxy Resins to Waxy Biological Tissues" DOI: https://doi.org/10.1017/S143192762001781X
Best regards - Joe
Joe Mowery | Biologist Electron and Confocal Microscopy Unit USDA Agricultural Research Service
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, August 13, 2020 8:59 AM To: Mowery, Joseph {joseph.mowery-at-usda.gov}
X-from: Kelley,Karen L {vau-at-ufl.edu}
I would recommend adding Z6040 to the resin mixture. I’ve used Z6040 in epoxy, LRW and HM20 (100ul/10ml resin). It will greatly improve the sectioning. Best of luck
Sent from my iPhone
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Good Morning/Afternoon/Evening Colleagues around the world...
The Microscopy Society of America has over many years been working on a detailed project to create a digital library containing copies of the proceedings of all our past meetings.
While our Archivist (Mike Marko) and several of us have a fairly extensive set of the (E)MSA and MM proceedings we are missing a few issues to complete the library.
Does anyone have copies of the following proceedings which we can borrow scan and then return?
We are looking for:
EMSA Proceedings 1961 - Pittsburg EMSA Proceedings 1965 - New York City EMSA Proceedings 1966 - San Francisco
Also if anyone has brochures/pamphlets advertising the 1962 Philadelpha or 1964 Detroit EMSA meetings we would also like to digitize and include them as part of our archives.
MSA will pay the cost for shipping, digitizing, and returning your proceedings.
To digitize the proceedings properly it will necessitate unbinding the hardcopy. The copies will, of course, be rebound and returned.
If you have copies that you can loan us, then please contact Mike Marko {mike.marko.em-at-gmail.com} and he will coordinate with you.
to repair a drifting focus situation at a tube-driven Zeiss EM9 S2 TEM still doing it`s work a a German high-school I am searching for the schematics on the generation of the lens currents.
I have part of it but I am missing a high-def PDF of:
- Lens Current Supply Functional circuit diagram 340600 Wart 64/65
- Lay-Out Ort 6 340600 Wart 31/32 (Component Layout Lens Current Supply - both sides)
- Lay-Out Ort 7 340600 Wart 29/30 (Component Layout Lens Shift - Astigmatism - Condensor - both sides)
It would be great if there is a chance to still get theses documents 55 years after the build.
All the best,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
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Message: When comparing Fe and Ni abundances within a suite of samples with identical crystal structures, prepared in the same way and analyzed under the same conditions (including same total counts in Fe and Ni):
1) Is the uncertainty associated with the Fe and Ni quantifications in each sample due solely to peak intensities (total integrated counts)?
2) Does the same uncertainty apply to each of the samples. Thanks and Aloha.
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Hi John, Are you using a standard less semi-quantitative routine or using standards? Most off the shelf EDS software packages are using standard less and they use k-ratios of peak intensities along with performing either ZAF or PhiRoZ corrections for absorption, fluoresce and atomic number. The standard less routines are much less sensitive to changes in beam current and sample geometry (working distance) compared to running pure elements standards. If you’re running pure standards there are a lot of variables that will influence the uncertainty of the measurement including beam current, working distance, sample flatness, etc.
Most EDS vendors running standardless account for beam current and working distance differences which means for all things being equal it’s sometimes easier to get consistent uncertainties when running a standard less quant routine vs. using elemental standards. At the end of the day it really depends on what type of analysis you’re running and what specifically you’re looking for.
actually I'm not 100% sure to have completely understood your question. The uncertainty is determined by different points, one is the measured net-count counting statistics. But these are total counts at peak area subtracted by background. So another part of uncertainty is the background consideration, the used method (differs by vendors). Just this becomes dominant with uncertainty if the concentration of the element is very small. Keeping with net-count measurement, this value can vary due to systematic errors, e.g. sample Z positioning or just you work with low magnification in SEM an select sample points once left top corner and next then below right. The absolute detector-geometry will change due to this (solid angle). And the beam-current is also a point of uncertainty (disappears at the end usually, if you normalize the Quant results). This is with the counts. If you mean finally the full quantitative results uncertainties (element concentrations), then systematic error will be due to the Quant model and the used method (e.g. with standards or standardless). If you evaluate standardless, then you can assume the systematic error (uncertainty) with the model will be actually same with all your similar samples. This is then indeed this systematic uncertainty applies to each of the samples then.
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X-from: johnbrad-at-hawaii.edu
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Message: When comparing Fe and Ni abundances within a suite of samples with identical crystal structures, prepared in the same way and analyzed under the same conditions (including same total counts in Fe and Ni):
1) Is the uncertainty associated with the Fe and Ni quantifications in each sample due solely to peak intensities (total integrated counts)?
2) Does the same uncertainty apply to each of the samples. Thanks and Aloha.
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I am not quite sure what the question is either. All we know about now is Fe and Ni. There was no mention of the rest of the sample other than it was consistent. What are the general levels of the elements? Is there anything that would be absorbing Ni more than Fe? What x-ray lines are being analyzed? What voltage was used?
Simply put, the precision has to do with the number of counts in the peak and background. For similar numbers for Fe and Ni, the precision should be the same. Of course, the background will be somewhat lower under Ni-K than Fe-K and the intensity of Ni will be less than Fe for the same mass fraction.
I would encourage you to get plenty of counts, period. We have many users in another lab that routinely fail to increase the beam current when doing x-ray analysis. Therefore, the uncertainties are much larger than they could be.
I have done plenty of standardless analysis and plenty of analyses with standards. I am not sure the differences are as much as Neal suggests. Differences in working distance and sample tilt (i.e., roughness) affect both types of analysis similarly. Beam current is usually an issue with standardized analyses since the results are often not normalized. Deviation of the total from 100% will reveal problems with the analysis. However, standardized analyses can be normalized (not necessarily advised) and the differences between the modes disappear.
Warren Straszheim, Ph.D., manager Materials Analysis and Research Lab 23 Town Engineering 515-294-8187
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Sunday, August 16, 2020 1:43 PM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
X-from: Neal Magdefrau {semrental-at-gmail.com}
Hi John, Are you using a standard less semi-quantitative routine or using standards? Most off the shelf EDS software packages are using standard less and they use k-ratios of peak intensities along with performing either ZAF or PhiRoZ corrections for absorption, fluoresce and atomic number. The standard less routines are much less sensitive to changes in beam current and sample geometry (working distance) compared to running pure elements standards. If you’re running pure standards there are a lot of variables that will influence the uncertainty of the measurement including beam current, working distance, sample flatness, etc.
Most EDS vendors running standardless account for beam current and working distance differences which means for all things being equal it’s sometimes easier to get consistent uncertainties when running a standard less quant routine vs. using elemental standards. At the end of the day it really depends on what type of analysis you’re running and what specifically you’re looking for.
} On Aug 16, 2020, at 11:10 AM, microscopy.listserver-at-gmail.com wrote: } } X-from: johnbrad-at-hawaii.edu } } This Question/Comment was submitted to the Microscopy Listserver using } the WWW based Form at } http://microscopy.com/MicroscopyListserver/MLFormMail.html } ---------------------------------------------------------------------- } ----- Remember this posting is most likely not from a Subscriber, so } when replying please copy to both johnbrad-at-hawaii.edu, } Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } ---------------------------------------------------------------------- } ----- } } Email: johnbrad-at-hawaii.edu Name: John Bradley } } Organization: HIGP University of Hawaii } } Title-Subject: [Filtered] Energy-dispersive x-ray quantification } } Message: When comparing Fe and Ni abundances within a suite of samples } with identical crystal structures, prepared in the same way and } analyzed under the same conditions (including same total counts in Fe and Ni): } } 1) Is the uncertainty associated with the Fe and Ni quantifications in } each sample due solely to peak intensities (total integrated counts)? } } 2) Does the same uncertainty apply to each of the samples. } Thanks and Aloha. } } Login Host: 72.130.218.27 } Listserver Email Form V - 20120416 } ---------------------------------------------------------------------- } ----- } } } ==============================Original } Headers============================== } 10, 53 -- From microscopy.listserver-at-gmail.com Sun Aug 16 09:55:42 } 2020 10, 53 -- Received: from mail-il1-f179.google.com } (mail-il1-f179.google.com [209.85.166.179]) 10, 53 -- by } microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 07GEtfNV025993 } 10, 53 -- for {microscopy-at-microscopy.com} ; Sun, 16 Aug 2020 } 09:55:41 -0500 10, 53 -- Received: by mail-il1-f179.google.com with
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Email: vau-at-ufl.edu Name: Karen Kelley
Organization: University of Florida
Title-Subject: [Filtered] HPF/FS archaea
Message: Does anyone have reference or methodology for HPF/FS of archaeal cells for enzyme detection and ultrastructure?
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Hello all, I wonder what other labs are doing to control the humidity in their Cryo EM experiments and scope operations. Are you dehumidifying the whole room with an expensive unit, or has someone figured out how to locally drop the humidity for prep and grid loading. Any comments are welcome. Also what are your target humidity numbers.
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Email: david.evanoff-at-maine.edu Name: David Evanoff
Organization: University of Maine
Title-Subject: [Filtered] Job Opportunity - Electron Microscopy Lab Manager
Message: The University of Maine (Orono Campus) seeks qualified applicants for a Microscopy Laboratory Manager. The centralized lab, which is open to any researcher at UMaine - Orono, as well as external clients, has SEM, TEM, FIB, and various LM capabilities. Please see the link below for more information and to apply.
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Message: Hi, I am pursuing my research in computational protein design. I have designed some inhibitory peptides (17 residues long). I am looking for the institute where get the training for cryo-EM and SPA method to validate the in-silico predicted peptide structures by the Cryo-EM SPA method. (I have an expertise on TEM operation at room temperature mode) It would be advantageous if I can get earn course credit hours for my Ph.D. degree through this course. Login Host: 129.130.146.20 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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Email: nyilmaz-at-mersin.edu.tr Name: sakir necat yilmaz Organization: Mersin University
Title-Subject: [Filtered] Grid holder for Jeol JEM1011
Message: Dear All,
We have a broken pin on the grid holder of our Jeol JEM 1011. Please check the link below for pictures. Do you have any idea to fix it or, can we find it as a spare part? Finally, we can happily accept your unused, old holder as a gift :))) Thanks in advance and best wishes from Turkey.
We just published a manuscript analyzing the use of microscopy and the reporting of imaging materials and methods in biomedical journals: https://elifesciences.org/articles/55133 The punch line can be summarized in the title: Imaging methods are vastly underreported in biomedical research Slightly off topic, and arguably shameless self-promotion, but we think the audience in this list is uniquely positioned to help solve the problem. Thanks for your interest. Best, Guillermo
Guillermo Marques University Imaging Centers University of Minnesota
==============================Original Headers============================== 2, 64 -- From marques-at-umn.edu Tue Aug 25 11:40:40 2020 2, 64 -- Received: from mta-p7.oit.umn.edu (mta-p7.oit.umn.edu [134.84.196.207]) 2, 64 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 07PGeeT0008443 2, 64 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Aug 2020 11:40:40 -0500 2, 64 -- Received: from localhost (unknown [127.0.0.1]) 2, 64 -- by mta-p7.oit.umn.edu (Postfix) with ESMTP id 4BbZhJ4p3Nz9xZ2g 2, 64 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Aug 2020 16:49:04 +0000 (UTC) 2, 64 -- X-Virus-Scanned: amavisd-new at umn.edu 2, 64 -- Received: from mta-p7.oit.umn.edu ([127.0.0.1]) 2, 64 -- by localhost (mta-p7.oit.umn.edu [127.0.0.1]) (amavisd-new, port 10024) 2, 64 -- with ESMTP id rEfjjzXEwxig for {Microscopy-at-microscopy.com} ; 2, 64 -- Tue, 25 Aug 2020 11:49:04 -0500 (CDT) 2, 64 -- Received: from dlp-agent-p1.oit.umn.edu (dlp-agent-p1.oit.umn.edu [134.84.189.201]) 2, 64 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-GCM-SHA384 (256/256 bits)) 2, 64 -- (No client certificate requested) 2, 64 -- by mta-p7.oit.umn.edu (Postfix) with ESMTPS id 4BbZhJ3C9Hz9xZ2f 2, 64 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Aug 2020 11:49:04 -0500 (CDT) 2, 64 -- DMARC-Filter: OpenDMARC Filter v1.3.2 mta-p7.oit.umn.edu 4BbZhJ3C9Hz9xZ2f 2, 64 -- DKIM-Filter: OpenDKIM Filter v2.11.0 mta-p7.oit.umn.edu 4BbZhJ3C9Hz9xZ2f 2, 64 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=umn.edu; 2, 64 -- s=20200309t; t=1598374144; 2, 64 -- bh=JBurNJK/vCpvNBhK4w2dFmPtzavkSq1oQxsriXywdfs=; 2, 64 -- h=From:Date:Subject:To:From; 2, 64 -- b=wHBFxBYQdOeiMCDaImkzl5in4Itp2852i7vn31KmrlCR8srwa4ajGpRy8bSvox3zY 2, 64 -- 2aiQESMX4cWqe8e2NrSAqrsJ5YSgxF3MMl0Rg17mC/h+F+zm2n47EROCwzMisFfk6J 2, 64 -- Y8XbONSYwn8q/HI5zsNaiWzAbYWoylJiyQmgYsLA= 2, 64 -- Received: from pps.filterd (dlp-agent-p1.oit.umn.edu [127.0.0.1]) 2, 64 -- by dlp-agent-p1.oit.umn.edu (8.16.0.42/8.16.0.42) with SMTP id 07PAiE9n017751 2, 64 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Aug 2020 09:49:04 -0700 2, 64 -- Authentication-Results: umn.edu; 2, 64 -- spf=pass smtp.mailfrom=marques-at-umn.edu; 2, 64 -- dkim=pass header.d=umn.edu header.s=google; 2, 64 -- dmarc=pass header.from=umn.edu 2, 64 -- Received: from mail-vk1-f197.google.com (mail-vk1-f197.google.com [209.85.221.197]) 2, 64 -- by dlp-agent-p1.oit.umn.edu with ESMTP id 333117974w-1 2, 64 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES128-GCM-SHA256 bits=128 verify=NOT) 2, 64 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Aug 2020 09:49:04 -0700 2, 64 -- Received: by mail-vk1-f197.google.com with SMTP id k185so3880669vke.10 2, 64 -- for {Microscopy-at-microscopy.com} ; Tue, 25 Aug 2020 09:49:04 -0700 (PDT) 2, 64 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 2, 64 -- d=1e100.net; s=20161025; 2, 64 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 2, 64 -- bh=JBurNJK/vCpvNBhK4w2dFmPtzavkSq1oQxsriXywdfs=; 2, 64 -- b=h7/Aq4wL2dItbee8UaH6fkIZcbz3VC+fLyQKcMRvbfUW9qiEb2p/+yCQwgKy070Lq1 2, 64 -- Cv7PENk46Ec5C/HXx+QYi7wi+xBJ/4rVrKH3beVUfudpeSw9SFaAEz+ag3gTVL1wfbzI 2, 64 -- vPJd8IenaftKSQB0JuUX1Mkow6Tmtgv4YdJLKWXvfXg1xl4MqRT74iyA/9Uabu8w+6Cp 2, 64 -- u9XCy/NqH6vCIjk8J3ixvczArEMbGyufFdmSu2A7U7vbTDWggZABukt3lHzq3I2NDV16 2, 64 -- U93lsP7/j2saLamq4ZMDH3eJzUCpgTyOVGhMnuc8xHcUmZHBtYeBb1GfXv4/RyFEcZcZ 2, 64 -- SKiA== 2, 64 -- X-Gm-Message-State: AOAM532mUmfipbNmHgg3kxIY5krmDxxXjWCiZ0W5ImdOUPISTWprZz8E 2, 64 -- +pJQzZa9O8HYWJVBu5PkfsWWpV1bGUMRBvoqqECtv5agt4vlp+aZoFD6VuKpWVuzsNpP/CPwIt5 2, 64 -- SU3FWqXw2hyvseYCt5DUZD2+ig3M2b/QBqFmotHEqwB3LOA== 2, 64 -- X-Received: by 2002:a05:6102:126f:: with SMTP id q15mr6286846vsg.160.1598374143290; 2, 64 -- Tue, 25 Aug 2020 09:49:03 -0700 (PDT) 2, 64 -- X-Google-Smtp-Source: ABdhPJwShYKokLQJNSPhoVfv6BvwUDObj7fszB9pCDXT0sGChr+a0qJ+8pBVOqEW7fPw3qdqWK0fPSRzerQO8U/g2ZA= 2, 64 -- X-Received: by 2002:a05:6102:126f:: with SMTP id q15mr6286836vsg.160.1598374143013; 2, 64 -- Tue, 25 Aug 2020 09:49:03 -0700 (PDT) 2, 64 -- MIME-Version: 1.0 2, 64 -- From: Guillermo Marques {marques-at-umn.edu} 2, 64 -- Date: Tue, 25 Aug 2020 11:48:51 -0500 2, 64 -- Message-ID: {CAKHVsvAenx9HTWB8aFOSCqE9NdRPGATsQ5=tdi1B-Ep3p3kxBg-at-mail.gmail.com} 2, 64 -- Subject: Microscopy materials and methods in publications 2, 64 -- To: Microscopy-at-microscopy.com 2, 64 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
From klostocc091qove-at-gmail.com Wed Aug 26 08:05:29 2020 Return-Path: {klostocc091qove-at-gmail.com} Received: from gmail.com ([104.148.61.164]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 07QD5S1P004544 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 26 Aug 2020 08:05:29 -0500 Message-ID: {F3977609.3054569C-at-gmail.com}
X-from: Araz qolizadeh {araz79az-at-yahoo.com}
Hi all I am working with EPMA Sx100, and unfortunately the Sp2,3,5 have no signals then we dont have analysis, these spectrometers stopped abruptly but not simultaneous, unfortunately, we did not reach a conclusion during this period, has anyone ever had such a problem and what could be the reason? thanks Kazem
X-from: Gilpin, Christopher J {gilpin-at-purdue.edu}
Tom Schmelzer at 724 453 3865 or tom-at-tgstechnologies.com is the go to for holder repairs. I am just a satisfied customer.
Chris He/Him/His Christopher J. Gilpin Ph.D. Campus-wide Coordinator for Electron Microscopy Director, Life Science Microscopy Facility Purdue University Whistler Hall of Agriculture Research, Room S052 170 S. University St West Lafayette, IN 47907 765-494-7750 gilpin-at-purdue.edu lsmf-at-purdue.edu reaches everyone in the facility. http://ag.purdue.edu/arp/Microscopy/Pages/default.aspx skype cjgilpin
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Tuesday, August 25, 2020 7:47 AM To: Gilpin, Christopher J {gilpin-at-purdue.edu}
X-from: nyilmaz-at-mersin.edu.tr
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Email: nyilmaz-at-mersin.edu.tr Name: sakir necat yilmaz Organization: Mersin University
Title-Subject: [Filtered] Grid holder for Jeol JEM1011
Message: Dear All,
We have a broken pin on the grid holder of our Jeol JEM 1011. Please check the link below for pictures. Do you have any idea to fix it or, can we find it as a spare part? Finally, we can happily accept your unused, old holder as a gift :))) Thanks in advance and best wishes from Turkey.
My only suggestion aside from finding a donor sample holder is to discover a good, strong, non-gassing epoxy. Or, if you are fortunate, a precision machine shop that could spot weld (very clean and precise) the stub back in place.
We have a JEOL 1011, but unfortunately only one holder. If you would like to use it in the future, we have a YouTube video on holder removal and insertion that you could use as a training video.
https://youtu.be/cYDP4C4LpNs
You might reach out to JEOL. Their crew is very very helpful and can probably suggest solutions other than purchasing another.
John Shields Managing Director Georgia Electron Microscopy
On Tue, Aug 25, 2020 at 7:57 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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Email: nyilmaz-at-mersin.edu.tr {mailto:nyilmaz-at-mersin.edu.tr} Name: sakir necat yilmaz Organization: Mersin University
Title-Subject: [Filtered] Grid holder for Jeol JEM1011
Message: Dear All,
We have a broken pin on the grid holder of our Jeol JEM 1011. Please check the link below for pictures. Do you have any idea to fix it or, can we find it as a spare part? Finally, we can happily accept your unused, old holder as a gift :))) Thanks in advance and best wishes from Turkey.
This is just an alignment pin. Most likely its base is screwed in on thread, less likely but possible - pressure fitted
In the threaded mating case - re-drill center of broken pin inside of the hole on a precision drill press and use small screw extractor to remove it. Once removed - use small lathe to manufacture a replacement pin from a suitable screw or even plain metal if you like cutting your own threads. Aluminum bronze would be my first choice, but free-machining phosphor bronze, titanium, or even OFHC copper should work in a pinch.
I the pressure fitting case - on a precision drill press drill out remains of the pin very closely to walls of the hole and pry remains out with a sharp steel needle. Once the hole is cleaned, use small lathe to make a replacement pin and drive it in with arbor press. Note that you would have to place entire rod into suitable tube holder base to prevent any possible damage or deformation while driving in a new pin.
I would try removing base of the pin with screw extractor first, and if it doesn't budge then assume it is pressure fitting and proceed with close drilling.
Best of luck! Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 www.partbeamsystech.com www.fibsemproducts.com www.freudlabs.com
"Only the Paranoid Survive" (A.Grove & SpaceX QA)
On 8/27/2020 8:08 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } X-from: John Shields {johnshields59-at-gmail.com} } } } Good morning Dr. Yilmaz,, } } My only suggestion aside from finding a donor sample holder is to discover a good, strong, } non-gassing epoxy. } Or, if you are fortunate, a precision machine shop that could spot weld (very clean and precise) the } stub back in place. } } We have a JEOL 1011, but unfortunately only one holder. If you would like to use it in the future, } we have a YouTube video on holder removal and insertion that you could use as a training video. } } https://youtu.be/cYDP4C4LpNs } } You might reach out to JEOL. Their crew is very very helpful and can probably suggest solutions } other than purchasing another. } } John Shields } Managing Director } Georgia Electron Microscopy } } On Tue, Aug 25, 2020 at 7:57 AM {microscopy.listserver-at-gmail.com } {mailto:microscopy.listserver-at-gmail.com} } wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: nyilmaz-at-mersin.edu.tr {mailto:nyilmaz-at-mersin.edu.tr} } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } nyilmaz-at-mersin.edu.tr {mailto:nyilmaz-at-mersin.edu.tr} , Microscopy-at-Microscopy.com so that  all } Microscopy Listserver Subscribers } can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: nyilmaz-at-mersin.edu.tr {mailto:nyilmaz-at-mersin.edu.tr} Name: sakir necat yilmaz } Organization: Mersin University } } Title-Subject: [Filtered] Grid holder for Jeol JEM1011 } } Message: Dear All, } } We have a broken pin on the grid holder of our Jeol JEM 1011. Please check the link below for } pictures. Do you have any idea to fix it or, can we find it as a spare part? 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Email: jpatrick-at-morgridge.org Name: Jan Patrick
Organization: Morgridge Institute for Research
Title-Subject: [Filtered] Postdoctoral Research Associate
Message: A Postdoctoral Research Associate position is available in the newly established Grant Laboratory within the Morgridge Institute for Research. The focus of the lab is the development of methods for single-particle cryo electron microscopy (cryo-EM) and cryo electron tomography (cryo-ET). The lab also develops the software package cisTEM (www.cistem.org), a user-friendly package for processing cryo-EM data. Requirements include: A Ph.D. in biology, biophysics, physics or a related field; experience in image / signal processing; experience with cryo-EM data processing is desired; experience with software programming. Ideally C++ programming and a strong publication record.
Qualified individuals interested in this opportunity are required to submit a cover letter and resume as one document via the link below.
From kipemilf56hoojl-at-gmail.com Fri Aug 28 06:39:35 2020 Return-Path: {kipemilf56hoojl-at-gmail.com} Received: from gmail.com ([104.148.61.171]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 07SBdYfw025900 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 28 Aug 2020 06:39:35 -0500 Message-ID: {97029998.628F4E68-at-gmail.com}
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Email: stanvitha-at-tamu.edu Name: Stanislav Vitha
Organization: Texas A&M University
Title-Subject: [Filtered] Re: de-humidifier Message: Hi Michael, we ended up dehumidifying the TEM room only. The original set up was with the building A/C system blowing the cold air under the floor (we have a 2 ft space between the floor and sub-floor) which then rises through several perforated floor tiles and is then extracted through the air return vents in the ceiling. The relative humidity for most of the year is around 55% at 72F. This is determined by the fact that the A/C system initially cools the air down to ~ 55 F, some water drops out and what is 100% RH at 55F is approximately 55% RH at 72F. After a $35k engineering study and a ~$500k cost estimate we decided to try something less costly first.
This was finished about 2 years ago. I purchased two small desiccant wheel dehumidifiers, the model was Munters HD DryCool (no longer available, but see my note below). They are a consumer model primarily for whole house dehumidification, not an industrial/heavy duty model, but for the price it seemed a good option to try. One important factor was its design, where they cool the incoming air and use the heat from it to regenerate the desiccant (you need to blow hot air on the dessicant to regenerate it). The major point is that the dehumidifiers do not heat up the processed air much (maybe 2-3 F), and thus the building A/C system is not trying to pump more cold wet air into the room to keep the set temperature.
The company that I bought the dehumidifier from, BMIL (https://bmil.com/ - no commercial interest, just a reasonably happy customer) was very helpful in running computer models to confirm the dehumidification performance for my proposed setup, also provided advice on sound-dampening ducting, ducting sizes, etc, ... So the overall solution was to intercept the incoming house air (typically ~55 to 65 F,), duct it to the service corridor next door where the dehumidifiers were installed, and return the dry and still cold air back into the room (under the floor) Because the incoming air flow is ~250 to 300 cfm, we needed to use two of the dehumidifiers, each could only handle about 150 cfm, to get a theoretical reduction from ~55 % RH down to ~30% RH.
In practical life it works reasonably well. At least initially we were achieving about 35% RH on normal hot humid Texas days. There may be some leaks of course, this is probably why we did not get down to 30%, but overall the cryo-TEM (200 kV FEI Tecnai) seemed happier in terms of the vacuum system and overall less ice buildup on cold surfaces of the holder during grid mounting and holder insertion (Gatan 626 holder, now also Fichione holder). All is not perfect, though, one of the dehumidifiers needed repair under warranty and then later the same unit had the compressor die out of warranty and had to be fixed. The overall performance seems to be drifting slowly towards higher humidity (currently 37 to 39 % RH on average). Some of it is also because these days during training, the door is propped open to allow for social distancing between the staff and the trainee in a relatively small room, or some users just do not care to keep the door closed. I do not know for sure, I do not do cryo-EM myself I am just a guy who likes to drill holes and construct things. I have some pictures of our current setup, as well as 3D drawing of the design that I could send if anybody is interested.
As I mentioned above, the Munters HD DryCool dehumidifiers are no longer available, but I was told (by the same company where we bought them) that there is a larger unit from a different manufacturer with 300 CFM capability. I wish we had this one when we did our project, it would have simplified the ducting, having just one instead of two units. The overall cost was about $10k for the two dehumidifiers, $1k for duct work inside the room (I ran the ducts myself) $500 for a replacement back door of the room in which I cut holes for the ducts, and about $20k to have the dehumidifiers professionally installed nextdoor - that included building a hanging rack for the two units, running 4 x 50 ft of solid metal duct with insulation, installing new electrical outlets, and ducting the hot wet regenerating air to the building exhaust system. I also spent another $350 for a networked Temp/humidity monitor so that myself and any of our users can check on their computer or phone the current or past Temp and RH. In a somewhat near future we will be doing room remodeling to accommodate a new cryo-FIB-SEM and it will also involve moving, among other things, the cryo-plunging apparatus (for cryo-TEM samples) into a new prep room where we may try reduce air humidity in a similar manner as I described above. Stan
Dr. Stanislav Vitha Microscopy and Imaging Center Texas A&M University ILSB 1131 College Station, TX 77843-2257
http://microscopy.tamu.edu
--------------------------------------------- Sent: Monday, August 24, 2020 1:55 PM
I am looking for the tripod polisher. If anyone has it on the shelf collecting dust - I will be happy to adopt it. Temporary borrowing options are also in consideration.
Please, email me lolita.rotkina-at-Yandex.com
Thank you very much!
Lolita Rotkina, Failure Analysis Engineer at TRUMPF Phitonics Inc.
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Email: john.hyun-at-ametek.com Name: John Hyun
Organization: Gatan Title-Subject: [Filtered] EELS & EFTEM Analysis Virtual Training School
Message: EELS & EFTEM Analysis Virtual Training School - October 19-23, 2020 School fee: $250.00 USD
This is a virtual EELS School for all levels. The focus of the training will be live, interactive microscope, and computer analysis sessions. The daily schedule for the 5-day school will include:
On-demand, pre-recorded lectures on key EELS and EFTEM topics. Q&A break sessions: Live text and video chats with instructors and participants after main sessions. Live microscope laboratories: Remote microscope sessions demonstrating next-generation EELS imaging and spectroscopy systems and software. These sessions will be recorded. Live software analysis laboratories: Live presentations, data analyses, and discussions. These sessions will be recorded. 24/7 text and video chat channels with instructors and participants. Complete training module: Lectures, laboratories, software demonstration software, sample data, product literature, and EELS Atlas app provided as downloads. Full access to the school virtual platform and materials during and 14 days after the school.
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Electron energy loss spectroscopy (EELS) is a powerful technique that provides both compositional and chemical information from sub-nanometer areas in the sample. As a course attendee, you will learn best practices to set up and optimize your EELS hardware and experimental protocols so you can capture and extract the maximum amount of compositional and chemical information from your TEM samples. Topics include:
Fundamentals of EELS and energy-filtered imaging in TEM Principles of operation of EFTEM and EELS systems Optimization of EFTEM and EELS data acquisition Quantification of elemental composition Other information provided by EFTEM/EELS and how best to extract it Use of EELS signals to form maps of elemental and chemical composition EFTEM and STEM EELS spectrum imaging techniques Identification of material phases via EELS fine structure mapping Applications to biological and physical science specimens Registration: https://www.gatan.com/company/events/eels-eftem-analysis-virtual-training-school-october-2020
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We are having a discussion in the lab about a possible caveat/issue using EDS to element map a mineral sample in SEM. Without disclosing too much about our project, I can say that the matrix is an alumosilicate which at one location is highly enriched in an element X. When an element mapping (including line scan) is performed at this location, all we can see in EDS is element X, Al and Si almost disappeared. Now the question is whether there really is no Al and Si at this location or whether they are present but cannot be detected because X is so enriched that it somehow hides Al and Si.
My opinion is that as long as the sensor is not saturated (deadtime } 0), Al and Si should be present in the spectrum, even though one may need to increase the dwell time significantly (except if X overlaps Si and/or Al but both is impossible I guess). My colleague reasoned using a "constant sum", which I don't know of, to explain why X can mask Si and Al. I would need expert opinion on this matter.
If X can really mask Si and Al in EDS, would WDS offer a solution?
Unless the peak from element X overlaps the Al & Si peaks, I would say that you should still see the matrix signal when the beam is next to the precipitate. Depending on the geometry of your system, if the generated X-rays pass through the precipitate on their way to the detector, there may be some significant absorption of Al & Si on one side of the precipitate.
If the precipitate is large enough, you have a reasonable dead time, and it is not showing Al & Si then I would say that those elements aren't in the phase. I'm not sure what your colleague means by "constant sum". The only thing I can think of is that, visually, if peak X grows quickly the Al & Si peaks shrink in comparison.
Cheers, Henk
----------------
Hendrik O. Colijn Center forElectronMicroscopy andAnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
-----Original Message----- X-from: nizets2-at-yahoo.com {nizets2-at-yahoo.com} Sent: Thursday, September 3, 2020 4:56 AM To: Colijn, Hendrik {colijn.1-at-osu.edu}
Stephane -
It would be nice to know the size of the location.
For instance, what if you had a 10um wide (and deep) cube of MgO included in the matrix?
We are having a discussion in the lab about a possible caveat/issue using EDS to element map a mineral sample in SEM. Without disclosing too much about our project, I can say that the matrix is an alumosilicate which at one location is highly enriched in an element X. When an element mapping (including line scan) is performed at this location, all we can see in EDS is element X, Al and Si almost disappeared. Now the question is whether there really is no Al and Si at this location or whether they are present but cannot be detected because X is so enriched that it somehow hides Al and Si.
My opinion is that as long as the sensor is not saturated (deadtime } 0), Al and Si should be present in the spectrum, even though one may need to increase the dwell time significantly (except if X overlaps Si and/or Al but both is impossible I guess). My colleague reasoned using a "constant sum", which I don't know of, to explain why X can mask Si and Al. I would need expert opinion on this matter.
If X can really mask Si and Al in EDS, would WDS offer a solution?
It's always hard to say much without full information. It would be nice to know what element X is and the rest of the composition. It might also help to know the EDS model involved and the instrumental conditions.
Mapping is not all that sensitive to changes in composition. Linescan is a little better, but spectra are the most sensitive to seeing small differences. I use mapping to reveal gross changes in chemistry. The noise per pixel obscures small changes, especially the way that I see lots of maps done.
You mention difficulties with mapping and you make a quick reference to linescan, but you did not say anything about regular spectra. Did you also collect them? That would provide a lot of information and might answer the questions. You did indicate if your sample does mapping with spectral imaging where all the spectrum is available at all points. If it did, then you could reconstruct the spectra for the phases and not go back and collect spectra specifically for the phases. I would really want to see the spectra. Is there something markedly different between the spectra? Is the Al and Si still present at a low level?
Suppose you did quant on the phases without normalization. Would the two areas give similar totals? If they did not, then something else is going on.
I appreciate Henk's comments in another reply. I also don't know what your colleague means by "constant sum". You have also said nothing about oxygen.
I think of two strange phenomena that have caused problems. I don't know if they might be at all related to your situation.
First, I remember someone giving a nice demonstration of the effect of count rate. I don't remember who it was, it has been many years, it might have been Chuck Fiore. The task was to map a copper TEM grid. They setup the SEM for brightness and count rate. They know they had to turn up the beam current from what was normally used for imaging so they setup for 30% deadtime using the entire field of view, maybe more. When they collected the map, they found Cu intensity in the holes of the grid and practically nothing on the bars of the grid. I should have said "Cu" intensity. It was the old school approach of mapping a region of interest without any background correction. When they were mapping the holes, they got some "Cu" intensity from the bremsstrahlung background. When they mapped the bars, the count rate shot up many times higher. If their input count rate was set for 20kcps over the entire grid, it probably jumped to 200 kcps on the bars and that effectively flooded the amplifier and shut it down so very few counts got out. So the holes had "Cu" and the grid bars had none.
Now I tried replicating this experience on an old Oxford ISIS, but I could not do it, per se. The ISIS corrected pixel dwell time for deadtime. So, if the deadtime went from 30 to 95%, my nominal dwell time of 10ms went from an actual dwell time of 14 ms to 200 ms (10ms*100%/(100%-95%). It gave the proper result, but the scanning was quite strange as the beam slowed to a crawl over the bars.
Second, I once tried analyzing a rare-earth phosphor that was severely cathodoluminescent. (Why should that be a surprise?) When the beam hit the phosphor, the count rate went through the roof. That meant dead time went to 100%. That shut down all output on a real-time basis. Even with dead-time correction, the analysis was impossible. In Oxford's defense, I cannot remember if this was their original detector or a replacement detector from Gresham. I do know that our current light-element detector is still subject to interference from light photons whether from the room or the IR camera in the SEM or from cathodoluminsence. Not knowing what element X is, I don't know if this might be relevant.
Warren Straszheim, Ph.D., manager Materials Analysis and Research Lab 23 Town Engineering 515-294-8187
-----Original Message----- X-from: nizets2-at-yahoo.com {nizets2-at-yahoo.com} Sent: Thursday, September 03, 2020 3:54 AM To: Straszheim, Warren E [BIOTC] {wesaia-at-iastate.edu}
Dear colleagues!
We are having a discussion in the lab about a possible caveat/issue using EDS to element map a mineral sample in SEM. Without disclosing too much about our project, I can say that the matrix is an alumosilicate which at one location is highly enriched in an element X. When an element mapping (including line scan) is performed at this location, all we can see in EDS is element X, Al and Si almost disappeared. Now the question is whether there really is no Al and Si at this location or whether they are present but cannot be detected because X is so enriched that it somehow hides Al and Si.
My opinion is that as long as the sensor is not saturated (deadtime } 0), Al and Si should be present in the spectrum, even though one may need to increase the dwell time significantly (except if X overlaps Si and/or Al but both is impossible I guess). My colleague reasoned using a "constant sum", which I don't know of, to explain why X can mask Si and Al. I would need expert opinion on this matter.
If X can really mask Si and Al in EDS, would WDS offer a solution?
Dear Listers, I would like to hear from any member who is proficient in unbiased design based stereological methods. I am looking for advice and help. Please contact privately.
Tom Bargar Electron Microscopy Specialist UNMC Electron Microscopy Core Facility 986395 Nebraska Medical Center Omaha, NE 68198-6395 tbargar-at-unmc.edu 402-559-7347
The information in this e-mail may be privileged and confidential, intended only for the use of the addressee(s) above. Any unauthorized use or disclosure of this information is prohibited. If you have received this e-mail by mistake, please delete it and immediately contact the sender.
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Title-Subject: [Filtered] DI3000/3100 AFM for Donation
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Located in Pittsburgh, PA Covid-19 protocols in place for pickup
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Hi, can anyone perform a one-off job for my client with a He-microscope now? It is about imaging pores in the material. They are really tiny: I could not detect any in my old SEM. Should be smaller than 5 nm. Cheers, Dimitri
/Dimitri Scholz, PhD, Dr. rer. nat. Director of Biological Imaging Conway Institute University College Dublin UCD Belfield, Dublin 4 Ireland Mobile: +353-87-7961547
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Email: fei.long-at-queensu.ca Name: Fei Long
Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for imaging
Message: Hi all,
I got a user want to do HRTEM on magnetite nanoparticles. My concern is that the nanoparticles will be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and contaminate the column. If anyone have similar experiences can provide advice which will be much appreciated. The sample is powder which I usually dilute with alcohol then apply a drop of the suspension onto a carbon coated grid. Fei Login Host: 209.183.28.3 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
Hi Fei, Some times ago, there was a discussion on this topic on the Microscopy ListServer. I'm sorry but I don't remember when. Please go to the Microscopy ListServer Archives and try to find it out: {http://microscopy.com/MicroscopyListserver/MicroscopyArchives.html}
Regards
Oldrich
-- Oldřich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group Vídeňská 1083 142 20 Prague 4 Czech Republic
On Wed, 9 Sep 2020 17:20:45 -0500, microscopy.listserver-at-gmail.com wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: fee.long-at-queensu.ca } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both fei.long-at-queensu.ca, } Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: fei.long-at-queensu.ca Name: Fei Long } } Title-Subject: [Filtered] Loading nano magnetic particles onto TEM } for imaging } } Message: Hi all, } } I got a user want to do HRTEM on magnetite nanoparticles. My concern } is that the nanoparticles will be pull away from the carbon coated } grid by the strong magnetic filed of the TEM lens, and contaminate } the column. If anyone have similar experiences can provide advice } which will be much appreciated. The sample is powder which I usually } dilute with alcohol then apply a drop of the suspension onto a carbon } coated grid. 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==============================Original Headers============================== 10, 29 -- From benada-at-biomed.cas.cz Thu Sep 10 01:49:03 2020 10, 29 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 10, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 08A6n2Tc025487 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 10 Sep 2020 01:49:02 -0500 10, 29 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 10, 29 -- (using TLSv1.3 with cipher TLS_AES_256_GCM_SHA384 (256/256 bits) 10, 29 -- key-exchange ECDHE (P-256) server-signature RSA-PSS (2048 bits) server-digest SHA256) 10, 29 -- (No client certificate requested) 10, 29 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 0553BD02E37; 10, 29 -- Thu, 10 Sep 2020 08:58:14 +0200 (CEST) 10, 29 -- Date: Thu, 10 Sep 2020 08:58:14 +0200 10, 29 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 10, 29 -- To: {microscopy-at-microscopy.com} , fee.long-at-queensu.ca 10, 29 -- Subject: Re: [Microscopy] viaWWW: Loading nano magnetic particles onto TEM 10, 29 -- for imaging 10, 29 -- Message-ID: {20200910085814.2285075c-at-u117ob02} 10, 29 -- In-Reply-To: {202009092220.089MKjUN023906-at-microscopy.com} 10, 29 -- References: {202009092220.089MKjUN023906-at-microscopy.com} 10, 29 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 10, 29 -- =?UTF-8?B?xIxS?= 10, 29 -- X-Mailer: Claws Mail 3.17.3 (GTK+ 2.24.32; i686-pc-linux-gnu) 10, 29 -- MIME-Version: 1.0 10, 29 -- Content-Type: text/plain; charset=UTF-8 10, 29 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 10, 29 -- X-IoP-CAS-MailScanner-ID: 0553BD02E37.AB679 10, 29 -- X-IoP-CAS-MailScanner: Processed 10, 29 -- X-Spam-Status: No 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 08A6n2Tc025487 ==============================End of - Headers==============================
The only "recent" Q/A via MSA-Listserver with regard to cleaning away "magnetic particles" I am aware of
was notification sent: Mon Mar 16 2020 17:34:02 How to clean nickel shim of magnetic and or glass particles? Q by Nathan McCorkle
with two replies ( find text below only for your convenience)
Regards,
Wolfgang Muss Retired MSA-Member SALZBURG AUSTRIA
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~ Q: From MSA-Listserver: Mon Mar 16 2020 17:34:02 I have a nickel shim destined for nanoimprint lithography, made by electroforming e-beam exposed photoresist. I don't have a proper cleanroom, but I've been trying to strip what seemed like residual ZEP e-beam resist...
And it has not been going so well. I've tried acetone, dichloromethane, n-methyl pyrrolidone, and 10% NaOH. Sonicated with heat in both acetone and NaOH (at different times). The NaOH is the most recent attempt, and it seemed to show improvement under FIB imaging, but I also noticed what appeared to be redeposition.
I can only imagine this is due to particulate in my solvents, dirty air as I blow dry the shim or carry it from my sonicator to my FIB desk, or maybe insoluble particles like glass or ferromagnetic dust which start to settle onto the sample as soon as the sonicator is turned off.
Features are around 150nm linewidth, high frequency and complex shaped... So lots of small say 500nm sized holes/crevices which I'd been thinking was just diffusion limited for the solvent to get into and do it's work. But now I'm pretty confused.
Should I invest in some .45 and .22 micron syringe filters for all my fluid work? Should I tape a magnet to the outside of the beaker I've been sonicating in, to try and collect such particles? What's a standard semiconductor lab method for cleaning magnetic particles from magnetic layers? How about the idea of insolubles? Or can someone recommend a solution that will etch glass but not nickel?
Thanks, -Nathan ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~ Re (1) From MSA-Listserver: Sat Mar 21 08:47:00 Hi Nathan, did you try using a plasma cleaner for cleaning the surfaces and also a plasma cleaner like the Evactron at the FIB chamber to keep the specimen clean during scanning? If you can mount the specimen with the surface to be cleaned facing down to the bottom of the beaker you might get rid of deposits coming from above ;-) Another try to clean the surfaces might be to plunge it in liquid nitrogen or to use a vacuum chamber with the cleaning solution and pump to a level below sublimation. And sure: clean micro-filtered solutions would help. Nickel and magnetism: you could use a demagnetizer to decrease / erase the magnetism in the shim first.
Best wishes, Stefan ----------------------------------------------------- Stefan Diller - Scientific Photography ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~ Re (2): From MSA-Listserver: Sat Mar 21 08:47:00 2020 Hi Nathan,
To clean a surface of particulates, I would use replicating tape. This is a cellulose acetate tape (non-adhesive) that you soften with acetone and press down onto the surface. Let it dry and peel it off. All the particulates should come with it. I've had better luck in removing particulates this way compared with ultrasonics, rinses, etc.
I'm not sure if an adhesive tape will work but if you don't have replicating tape, you might try some of the tape with the "Post-it" type adhesive. It may take several applications to remove everything.
Replicating tape is available from most of the EM supply houses. It comes in both a thick and thin form.
Cheers, Henk ---------------- Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ ~~~~~~~~~~~~~~~~~~~~~~~~~~~
-----Ursprngliche Nachricht----- Von: benada-at-biomed.cas.cz [mailto:benada-at-biomed.cas.cz] Gesendet: Donnerstag, 10. September 2020 08:50 An: wij.muss-at-aon.at Betreff: [Microscopy] Re: Loading nano magnetic particles onto TEM
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Regards
Oldrich
-- Oldřich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group Vídeňská 1083 142 20 Prague 4 Czech Republic
On Wed, 9 Sep 2020 17:20:45 -0500, microscopy.listserver-at-gmail.com wrote : } } } } ---------------------------------------------------------------------- } ------ The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------- } ------ } } X-from: fee.long-at-queensu.ca } } This Question/Comment was submitted to the Microscopy Listserver using } the WWW based Form at http://www.microscopy.com/MLFormMail.html } ---------------------------------------------------------------------- } ----- Remember this posting is most likely not from a Subscriber, so } when replying please copy to both fei.long-at-queensu.ca, } Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } ---------------------------------------------------------------------- } ----- } } Email: fei.long-at-queensu.ca Name: Fei Long } } Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for } imaging } } Message: Hi all, } } I got a user want to do HRTEM on magnetite nanoparticles. My concern } is that the nanoparticles will be pull away from the carbon coated } grid by the strong magnetic filed of the TEM lens, and contaminate the } column. If anyone have similar experiences can provide advice which } will be much appreciated. The sample is powder which I usually dilute } with alcohol then apply a drop of the suspension onto a carbon coated } grid. Fei Login Host: 209.183.28.3 } Listserver Email Form V - 20120416 } ---------------------------------------------------------------------- } ----- } } } ==============================Original } Headers============================== 7, 53 -- From } microscopy.listserver-at-gmail.com Wed Sep 9 17:20:19 2020 7, 53 -- } Received: from mail-il1-f177.google.com (mail-il1-f177.google.com } [209.85.166.177]) 7, 53 -- by microscopy.com } (8.12.11.20060308/8.12.8) with ESMTP id 089MKJMu023537 7, 53 -- } for {microscopy-at-microscopy.com} ; Wed, 9 Sep 2020 17:20:19 } -0500 7, 53 -- Received: by mail-il1-f177.google.com with SMTP id } t13so3870439ile.9 7, 53 -- for {microscopy-at-microscopy.com} ; } Wed, 09 Sep 2020 15:29:33 -0700 (PDT) 7, 53 -- DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 7, 53 -- d=gmail.com; } s=20161025; 7, 53 -- } h=subject:references:to:from:message-id:date:user-agent:mime-version } 7, 53 } -- :in-reply-to:content-language:content-transfer-encoding; } 7, 53 -- bh=Kk8kB5glY/9vHet2a2EwaXc3NJAEVkj7IAsVV8/r9yA=; 7, } 53 -- } b=AQ4MAPMJP2zVpg94IzWTmPUQ4atnqzdGcFOxEhhahgdi0NrSWPrL1nK8JpuhtyqGyI } 7, 53 -- } 5WIQG7jVT8xZUmXGt886KIUZhkp8i///VRCkcC8hGOcv9ePp/vdMQjhMp13sgkUUz/ll } 7, 53 -- } qd7odJbew8ivFkSJYYg7giDkzuZ13Ez/451rnFPIwQV26ibN//n2ollQ073FUkEBjDnF } 7, 53 -- } qThJyoqtGfHFKNOCboDnguYceqFWthtEEJmoGpq7ZQGPoOJXzyYUQiYq6+RtGpSo6Ym9 } 7, 53 -- } PIQRKL6O4D5XtynY9/4bFIOclt02Wrmib3SfGSFbcyNfZS6d96SEIJHLosn3S53QCJ2r } 7, 53 -- PjYA== 7, 53 -- X-Google-DKIM-Signature: v=1; } a=rsa-sha256; c=relaxed/relaxed; 7, 53 -- d=1e100.net; } s=20161025; 7, 53 -- } h=x-gm-message-state:subject:references:to:from:message-id:date 7, 53 } -- :user-agent:mime-version:in-reply-to:content-language 7, } 53 -- :content-transfer-encoding; 7, 53 -- } bh=Kk8kB5glY/9vHet2a2EwaXc3NJAEVkj7IAsVV8/r9yA=; 7, 53 -- } b=LNKbG+0nKJIRnVbUFUHApsTIlqF5w5clfbqLmQL1IMW1y4J/JReP2vqacTd+NQp/wZ } 7, 53 -- } P2slKw0irSi0iTlM3FFU1LIdBpqR92wJE1vnQK1hRWSmd/c6PHNbALhEZ12e3qswU69q } 7, 53 -- } DOMzO3AQzFkrn6XFFAF7zUj573P2wnHbZBYaUHQ93u4dFYyvcif5W64nGKTJlOdpxYri } 7, 53 -- } JppCS1mEE0exjK1sZJbhUbC9LpZ67AkY6Hd9ZhRvkqODXudCF9qW4Vqw8Xv5i7Tg63Dg } 7, 53 -- } kk45SL9e4G7AeZixIMcDihZquwZZkd1rcppDVYVbieqnVA/QhKT5jfUN6B1CMAUKQhL8 } 7, 53 -- 7O1A== 7, 53 -- X-Gm-Message-State: } AOAM532OMinlOhyeU26nMNyi3YQJzk6VWCrEBJcY5ZiE+Q63eNdfVOW+ 7, 53 -- } UzoppYy8LIWG7nUMjx4sydT8DcyQoLY= 7, 53 -- } X-Google-Smtp-Source: } ABdhPJxzcoCZs0Jx4QzHzXuM6d0Ue/2eSjH/FdG2UU7IHfD+BKT2Om3E9MmgkIeXFYQdyc } WReDL/TA== 7, 53 -- X-Received: by 2002:a92:58cd:: with SMTP id } z74mr5773583ilf.224.1599690572784; 7, 53 -- Wed, 09 Sep 2020 } 15:29:32 -0700 (PDT) 7, 53 -- Received: from } 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:f06e:3b12:cc26:1638]) 7, 53 -- by } smtp.googlemail.com with ESMTPSA id } z15sm2083121ilb.73.2020.09.09.15.29.31 7, 53 -- for } {microscopy-at-microscopy.com} 7, 53 -- (version=TLS1_3 } cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 7, 53 -- Wed, 09 } Sep 2020 15:29:31 -0700 (PDT) 7, 53 -- Subject: viaWWW: Loading nano } magnetic particles onto TEM for imaging 7, 53 -- References: } {202009091839.089IdHuj013872-at-microscopy.com} 7, 53 -- To: } MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 53 -- } From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 7, 53 -- } X-Forwarded-Message-Id: {202009091839.089IdHuj013872-at-microscopy.com} } 7, 53 -- Message-ID: {e4633ef8-aaff-c5be-4404-93b619b016fc-at-gmail.com} } 7, 53 -- Date: Wed, 9 Sep 2020 17:29:30 -0500 7, 53 -- User-Agent: } Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) 7, 53 -- } Gecko/20100101 Thunderbird/68.12.0 7, 53 -- MIME-Version: 1.0 7, 53 } -- In-Reply-To: {202009091839.089IdHuj013872-at-microscopy.com} 7, 53 -- } Content-Type: text/plain; charset=windows-1252; format=flowed 7, 53 } -- Content-Language: en-US 7, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - } Headers==============================
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==============================Original Headers============================== 10, 29 -- From benada-at-biomed.cas.cz Thu Sep 10 01:49:03 2020 10, 29 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 10, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 08A6n2Tc025487 10, 29 -- for {microscopy-at-microscopy.com} ; Thu, 10 Sep 2020 01:49:02 -0500 10, 29 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 10, 29 -- (using TLSv1.3 with cipher TLS_AES_256_GCM_SHA384 (256/256 bits) 10, 29 -- key-exchange ECDHE (P-256) server-signature RSA-PSS (2048 bits) server-digest SHA256) 10, 29 -- (No client certificate requested) 10, 29 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 0553BD02E37; 10, 29 -- Thu, 10 Sep 2020 08:58:14 +0200 (CEST) 10, 29 -- Date: Thu, 10 Sep 2020 08:58:14 +0200 10, 29 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 10, 29 -- To: {microscopy-at-microscopy.com} , fee.long-at-queensu.ca 10, 29 -- Subject: Re: [Microscopy] viaWWW: Loading nano magnetic particles onto TEM 10, 29 -- for imaging 10, 29 -- Message-ID: {20200910085814.2285075c-at-u117ob02} 10, 29 -- In-Reply-To: {202009092220.089MKjUN023906-at-microscopy.com} 10, 29 -- References: {202009092220.089MKjUN023906-at-microscopy.com} 10, 29 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 10, 29 -- =?UTF-8?B?xIxS?= 10, 29 -- X-Mailer: Claws Mail 3.17.3 (GTK+ 2.24.32; i686-pc-linux-gnu) 10, 29 -- MIME-Version: 1.0 10, 29 -- Content-Type: text/plain; charset=UTF-8 10, 29 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 10, 29 -- X-IoP-CAS-MailScanner-ID: 0553BD02E37.AB679 10, 29 -- X-IoP-CAS-MailScanner: Processed 10, 29 -- X-Spam-Status: No 10, 29 -- Content-Transfer-Encoding: 8bit 10, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 08A6n2Tc025487 ==============================End of - Headers==============================
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X-from: Ravi Thakkar {ravi.thakkar369-at-gmail.com}
Hi Long, You can use another carbon-coated grid on top of the grid loaded with magnetic nanoparticles. With Thanks and Regards. ------------------------------------------------------------------------- Ravindra Thakkar Associate Scientist, Kansas State University, Manhattan, Kansas (USA) -------------------------------------------------------------------------
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Email: fei.long-at-queensu.ca {mailto:fei.long-at-queensu.ca} Name: Fei Long
Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for imaging
Message: Hi all,
I got a user want to do HRTEM on magnetite nanoparticles. My concern is that the nanoparticles will be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and contaminate the column. If anyone have similar experiences can provide advice which will be much appreciated. The sample is powder which I usually dilute with alcohol then apply a drop of the suspension onto a carbon coated grid. Fei Login Host: 209.183.28.3 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
X-from: Michel Ribardire {m.ribardiere-at-jeol.fr}
The emf is here if you have magneric field and movement. So insert sample without magnetic field then use microscope with very slow movement. For retracting sample use same way (no magnetic field) Michel
Envoy de mon Galaxy A5 2017 Orange
-------- Message d'origine -------- De : microscopy.listserver-at-gmail.com Date : 10/09/2020 00:30 (GMT+01:00) : Michel Ribardire {m.ribardiere-at-jeol.fr} Objet : [Microscopy] viaWWW: Loading nano magnetic particles onto TEM for imaging
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Email: fei.long-at-queensu.ca Name: Fei Long
Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for imaging
Message: Hi all,
I got a user want to do HRTEM on magnetite nanoparticles. My concern is that the nanoparticles will be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and contaminate the column. If anyone have similar experiences can provide advice which will be much appreciated. The sample is powder which I usually dilute with alcohol then apply a drop of the suspension onto a carbon coated grid. Fei Login Host: 209.183.28.3 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This is indeed NOT a good idea, the particles WILL be captured by the lenses. I am afraid the user needs to find another method. An alternative may be to embed them and analyze sections? Be finding the right dilution, one should be able to image only 1 isolated particle.
Regards, Stephane
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Email: fei.long-at-queensu.ca Name: Fei Long
Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for imaging
Message: Hi all,
I got a user want to do HRTEM on magnetite nanoparticles. My concern is that the nanoparticles will be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and contaminate the column. If anyone have similar experiences can provide advice which will be much appreciated. The sample is powder which I usually dilute with alcohol then apply a drop of the suspension onto a carbon coated grid. Fei Login Host: 209.183.28.3 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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There seems to be some significant misunderstanding of the scale of forces applied by the 'strong magnetic field' inside a TEM. I've been looking at magnetic nanoparticles of various kinds in every type of TEM/STEM system for many years and never had any problems.
The van der waals forces - the electrostatics sticking your particles to a grid are VASTLY stronger (several orders of magnitude) than the magnetic moment for micro or nanoparticles inside a TEM. As long as you are not dumping vast amounts of this material onto your grid (a good rule of thumb is if you can see anything by eye in the suspension or on the grid after deposition) you are generally safe. A good approach to be really sure is to pass a rare earth magnet over the sample after deposition to pick up any larger boulders/chunks (thanks Nestor).
The real risk for magnetic materials in TEM's is where a bulk specimens can be become magnetised as a single or set of aligned domains (such as a 3mm chunk of ferritic steel), then the combined magnetic moment can tear a sample from a spring (jesus) clip. A safe holder to use in these cases is always a screwed hexnut.
Best of luck,
Matthew
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-- Dr Matthew Weyland Joint Deputy Director - Monash Centre for Electron Microscopy Associate Professor - Department of Materials Science and Engineering
Monash Centre for Electron Microscopy 10 Innovation Walk, Clayton Campus Monash University Clayton VIC 3800 Australia
For a properly loaded TEM sample, the risk of nanoparticles being ripped from the support is very low. By properly loaded, I mean that a small amount of particles are evenly distributed across a TEM support grid. You want to avoid clumps that are visible in a stereoscope or microscope. Large clumps can be weakly adhered, or not adhered at all, and could be pulled to one of the pole pieces. Your plan to dilute with a solvent and drop onto a TEM grid is perfectly reasonable. Just check it in an optical microscope to make sure you don't have any clumps. If you do, dilute and repeat on a new grid.
I've looked at countless magnetite (and other magnetic NPs) and have never observed any particles being removed from the support film. On the TEMs I've used to image these samples, I've inspected the objective pole pieces carefully during service and they were clean, at least at the level of observation permitted by an optical microscope. You can monitor the objective stigmator values over time to see if you're getting gross contamination, but it would take a *very* large number of NPs/clumps to make a perceivable impact. Now, bulk magnetic samples are a very different story! I can tell you stories about "hairy" pole pieces and magnetic samples making their home inside a pole piece (I'm guilty of the latter, I'm afraid to admit).
Good luck, Chris
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My concern is that the nanoparticles will } be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and } contaminate the column. If anyone have similar experiences can provide advice which will be much } appreciated. 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-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
I have done what Ravi suggests and it works fine. Just takes a little time to align the grid bars of the two overlapping grids.
Roy Geiss Research Staff ARC-ISS Colorado State University Fort Collins, CO ---------------------------------------------------------------------------------------------------- *From:* microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} *Sent:* Thursday, September 10, 2020 7:42 AM *To:* Geiss,Roy {Roy.Geiss-at-colostate.edu} *Subject:* [Microscopy] Fwd: Re: viaWWW: Loading nano magnetic particles onto
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X-from: Ravi Thakkar {ravi.thakkar369-at-gmail.com}
Hi Long, You can use anothercarbon-coated grid on top of the grid loaded with magnetic nanoparticles. With Thanks and Regards. ------------------------------------------------------------------------- Ravindra Thakkar Associate Scientist, Kansas StateUniversity, Manhattan, Kansas (USA) -------------------------------------------------------------------------
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Email: fei.long-at-queensu.ca {mailto:fei.long-at-queensu.ca} Name: Fei Long
Title-Subject: [Filtered] Loading nano magnetic particles onto TEM for imaging
Message: Hi all,
I got a user want to do HRTEM on magnetite nanoparticles. My concern is that the nanoparticles will be pull away from the carbon coated grid by the strong magnetic filed of the TEM lens, and contaminate the column. If anyone have similar experiences can provide advice which will be much appreciated. The sample is powder which I usually dilute with alcohol then apply a drop of the suspension onto a carbon coated grid. Fei Login Host: 209.183.28.3 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
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The Stanford Nano Shared Facilities (SNSF) is seeking a Transmission Electron Microscopy (TEM) scientist to lead the operations of the facilities' Transmission Electron Microscopes, including the TF Titan and Tecnai TEMs. The TEM scientist will provide training and support to researchers, maintain and optimize the microscope operation, work closely with equipment vendors, maintain and develop training procedures, develop and implement advanced techniques, assist and advice users on specimen preparation and data analysis, provide proof-of-concept service, and engage in research activities. The SNSF Titan ETEM is equipped with a spherical aberration corrector, EELS and EDS, a monochromator and high brightness gun, Lorentz, holography, and tomography capabilities. The Tecnai TEM has a field-emission gun, STEM unit with bright field/dark field detector and EDS detectors. Both instruments are compatible with a full suite of in situ holders. The TEM scientist will work to utilize this range of advanced techniques to the highest extent, making them known to the user community, matching appropriate techniques to the individual research projects. S(he) will interact with the broader research community and equipment vendors to be aware of advances in the field, and make recommendations and prepare proposals for future equipment purchases. S(he) will promote and collaborate in the publication and presentation of results.
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Title-Subject: [Filtered] Postdoctoral position offer IMN NANTES FRANCE - 3D Cryo FIB/SEM
Message: Hello !
A Postdoctoral Research position is available in the IMN Nantes laboratory within the University of Nantes-FRANCE.The focus of the lab is the development of caracterisation methods for material science. Here, this is about the development of cryos FIB/SEM microscopy techniques for caracterise innovative tissue engineered biomaterials with stemcells encapsulated inside the biomaterials’ structure.
All the informations to apply are in the link : https://emploi.cnrs.fr/Offres/CDD/UMR6502-PATABE-001/Default.aspx?lang=EN
Don't hesitate to share around you, the requierment include a good experience and skills in FIB/SEM microscopy techniques, knowlegde on cryo FIB/SEM techniques is a nice advantage.
The team is cool, don't hesitate !
Thank you and have a nice day
Hélène Roberge Phd student IMN - Institut des Matériaux Jean Rouxel 2, rue de la Houssinière - BP 32229 44 322 NANTES CEDEX 3 https://www.cnrs-imn.fr/ 0240376316
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Hello all,
We've recently moved to non-radioactive staining agents and are finding that these alternatives generally offer less contrast than their radioactive counterparts. In an effort to improve imaging of these lower contrast materials, I've tried to think of every major way to increase contrast from the TEM side of things. Our TEMs are materials 'scopes and we don't have access to a dedicated bio TEM, so I'm working with what I have.
I've moved to lower voltages, from 200 to 80kV, used as small of an objective aperture as I can get away with, applied a generous amount of defocus during imaging, and used the GIF to form images from the elastic contribution.
Am I missing anything major besides digital manipulation of brightness, contrast, and gamma on the images? Is it worth spending the time and effort to align at 20kV to improve contrast in thin sections?
Thanks for any advice you can share, Chris
-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
I was wondering if there would be a way to quantify (NOT detect!!) viral particles by EM (TEM or SEM). Personally I cannot see one, the main probably being to assess the number of viral particles quantitatively. Perhaps by SEM but the viral particles are approx. 100nm in diameter, which I couldn't resolve with our basic W-SEM.
Have somebody some experience with this topic? Best regards, Stephane
I'm not sure what you mean by "GIF" in this case (I'm assuming you don't mean the file format), but if this isn't an aperture ... since you have materials 'scopes, you have a diffraction/field of view aperture. You can use this aperture to increase the contrast without any resolution loss (since this aperture isn't in the objective lens). It will give you a bit of resolution improvement by removing peripheral electrons, but the main negative effect is the vignetting. On our TEM, we can't use even the largest (200 µm) below ~10kX. The contrast increase is less than that from using the objective aperture, but it's noticeable.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office
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Title-Subject: [Filtered] HT tank for our Transmission Electron Microscope
Message: Dear We need to purchase the HT tank for our HRTEM (FEI, TF30, STWIN ) equipped with a Field Emission Gun (FEG) gun and operating at 300 keV accelerating voltage. For this we need to repair/replace our faulty HT tank under buyback scheme. Please do needful if possible for you
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I guess I don't understand how the SA aperture increases contrast since it is in an image plane not a diffraction plane. You are referring to the SA aperture, right?
As a note... GIF is "Gatan Imaging Filter". Chris is trying to improve contrast by energy-filtering.
Cheers, Henk
----------------
Hendrik O. Colijn Center forElectronMicroscopy andAnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
colijn.1-at-osu.edu 614/643-3458 cemas.osu.edu
-----Original Message----- X-from: oshel1pe-at-cmich.edu {oshel1pe-at-cmich.edu} Sent: Thursday, September 17, 2020 8:03 AM To: Colijn, Hendrik {colijn.1-at-osu.edu}
Hello Phil,
I have the same question as Henk. Are you referring to the aperture that is located at the intermediate crossover in JEOL microscopes? I do miss that aperture because you could use it as a pseudo objective to increase contrast in TEM mode, improve BF contrast in STEM mode, and most usefully employ it during EDS. Or do you mean using the selected area in low mag to improve contrast?
As Henk said, the GIF is the Gatan Energy Filter.
Thanks, Chris
On Thu, Sep 17, 2020 at 8:07 AM Oshel, Philip Eugene {oshel1pe-at-cmich.edu} wrote: } } Chris, } } I'm not sure what you mean by "GIF" in this case (I'm assuming you don't mean the file format), but if this isn't an aperture ... since you have materials 'scopes, you have a diffraction/field of view aperture. You can use this aperture to increase the contrast without any resolution loss (since this aperture isn't in the objective lens). It will give you a bit of resolution improvement by removing peripheral electrons, but the main negative effect is the vignetting. On our TEM, we can't use even the largest (200 µm) below ~10kX. The contrast increase is less than that from using the objective aperture, but it's noticeable. } } Phil } ------------- } Philip Oshel } Imaging Facility Director } Biology Department } 1304 Biosciences } 1455 Calumet Ct. } Central Michigan University } Mt. Pleasant, MI 48859 } 989 774-3576 office } } -----Original Message----- } From: "crwinkler-at-ncsu.edu" {crwinkler-at-ncsu.edu} } Reply-To: "crwinkler-at-ncsu.edu" {crwinkler-at-ncsu.edu} } Date: Wednesday, 16September, 2020 at 21:57 } To: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu} } Subject: [External] [Microscopy] Contrast enhancement strategies in TEM? } } Hello all, } } We've recently moved to non-radioactive staining agents and are } finding that these alternatives generally offer less contrast than } their radioactive counterparts. In an effort to improve imaging of } these lower contrast materials, I've tried to think of every major way } to increase contrast from the TEM side of things. Our TEMs are } materials 'scopes and we don't have access to a dedicated bio TEM, so } I'm working with what I have. } } I've moved to lower voltages, from 200 to 80kV, used as small of an } objective aperture as I can get away with, applied a generous amount } of defocus during imaging, and used the GIF to form images from the } elastic contribution. } } Am I missing anything major besides digital manipulation of } brightness, contrast, and gamma on the images? Is it worth spending } the time and effort to align at 20kV to improve contrast in thin } sections? } } Thanks for any advice you can share, } Chris } } -- } Transmission Electron Microscopy Lab Manager } Analytical Instrumentation Facility (AIF) } NC State University } https://www.aif.ncsu.edu/ } Cell: 267-496-0587 } } ==============================Original Headers============================== } 6, 49 -- From crwinkler-at-ncsu.edu Wed Sep 16 20:42:49 2020 } 6, 49 -- Received: from mail-io1-f48.google.com (mail-io1-f48.google.com [209.85.166.48]) } 6, 49 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 08H1gnIe017464 } 6, 49 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Sep 2020 20:42:49 -0500 } 6, 49 -- Received: by mail-io1-f48.google.com with SMTP id y74so387893iof.12 } 6, 49 -- for {Microscopy-at-microscopy.com} ; Wed, 16 Sep 2020 18:52:26 -0700 (PDT) } 6, 49 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 49 -- d=ncsu.edu; s=google; } 6, 49 -- h=mime-version:from:date:message-id:subject:to; } 6, 49 -- bh=zZf3GyyS775gswhkuqP9SD27O1U7tia/j7RcBxYfVeU=; } 6, 49 -- b=aHAYqNkuZ/YklrD03wC/ZsC7Uz+LXukq/M7t0zRtRCyCylfDFAIEroWS4LY8Hy4O9o } 6, 49 -- 0HVY3Eq/rl0gE0rV63zZh2QTdNMAPokkKuCIUu/UAt+hg8PpvboUgqxQgfFpl5kdW2f6 } 6, 49 -- NreKmQgBn9nVAgRaT2wke/1YEGlwS9gjRxXjsR6i8+13VkkctL80JcFG5ct06Rsjj0YX } 6, 49 -- qEwKrCr8cHYFKu1Oz46lpGde5HvcZQk2ahjnUC4B78oDyQILT6wHh7ur0UdmUnblBgfz } 6, 49 -- 8fhumcpss79BjVMI0Xp1T3FYzKUHCyLlB7ATdmr/nk6WubaROCvQ1HFupy8jDwLcDoBR } 6, 49 -- yA0g== } 6, 49 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 6, 49 -- d=1e100.net; s=20161025; } 6, 49 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; } 6, 49 -- bh=zZf3GyyS775gswhkuqP9SD27O1U7tia/j7RcBxYfVeU=; } 6, 49 -- b=ikam2InZNiEj1w43bi3umdbpl0jkStG2JCRPwCiOAnoViTe07lZ79mIvTGeoIYYWwM } 6, 49 -- p/abqM7fgG9ui1YFXkwgcrTewvtavMdMzj3lKkWPTPj53hVDrt97VcjA1zEbDY0pYujB } 6, 49 -- BG+PYy1dFLnMttHaLAgD5Si1V5Rme4BMDoeZdR67EUpQRbI3SOax/vk1qaZNHBGrKBm7 } 6, 49 -- eobqqze7ipSYc1MQWiQJslXJpNdLz225w1c9LXKaRsTxUGlG8aH9UUFGwWvnQ3JDT2NW } 6, 49 -- ceNLhkaF6pOgmw9SdddIajXpTFi3GArZDPmSOW7sSeGhoWm/k/UzZv8AAIIxitWq2my0 } 6, 49 -- GEhg== } 6, 49 -- X-Gm-Message-State: AOAM532YOmqfdQ8FS+El+lFJXb20WbgdditQZvVe9V1xWrxeoXu94UPh } 6, 49 -- CcXe+5liBBhFkBrPrsuMzAp/ZvLP/Qowke1CY+t/gBWgVL6bH1JG105gPblk4+jtVNT0MD6unYz } 6, 49 -- boasTVPJEJFWqZiz/CMaak40kN3O81ShYnG0KGy3WC1ztextnxCDzngoReayenHMm2zYspiI= } 6, 49 -- X-Google-Smtp-Source: ABdhPJyI5LtT2FCyLZs2dxRu2UDIGktkfFH+0vl+5jWL4DT1KtB6EN2Y2pol4kxAdnfZo2Z3X7u9MQ== } 6, 49 -- X-Received: by 2002:a05:6602:2e87:: with SMTP id m7mr21112323iow.106.1600307546224; } 6, 49 -- Wed, 16 Sep 2020 18:52:26 -0700 (PDT) } 6, 49 -- Received: from mail-io1-f43.google.com (mail-io1-f43.google.com. 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-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
I mean the selected-area (SA) aka diffraction aka Hitachi's FOV (field-of-view) aperture. It increases contrast just because it blocks strays and peripheral electrons (like the fixed apertures do), and helps cut aberration a bit by blocking peripheral electrons. Just a side effect of being an aperture. Doesn't matter if it's in an image plane or a diffraction plane. Not a low mag aperture. (I'd have to look at a column schematic to know if this is where the intermediate crossover is in a JEOL. It's below the objective lens, at the first intermediate in the Hitachi 7700.) The effect isn't as strong as with an aperture specifically placed to increase contrast (in the objective lens), but it's still there.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office
-----Original Message----- X-from: Christopher Winkler {crwinkler-at-ncsu.edu}
Hello Teresa,
Thank you for the paper and the protocol. I'll pass it along to the scientist in charge to sample prep.
The idea about switching to STEM in the SEM is quite interesting. Thank you! That's definitely something we will test out.
Cheers, Chris
On Thu, Sep 17, 2020 at 10:26 AM Sawyer, Teresa {sawyerte-at-science.oregonstate.edu} wrote: } } Hello Chris, } } OSU also only has an analytical TEM and it is difficult to image } biologicals. Luck for me I can do STEM imaging on one of our SEM. With } a larger field of view and the extra staining I do it works out great. } } I was introduced to O-T-O-T-O staining a few years back and now I use it } all the time. You might not need to do all three osmium staining but } this method has eliminated the UA or alternative from the protocol. } } I have attached the protocol but I have modified it some over the years. } I cut the osmium staining time in half. } } I can not stress enough how much easier my life is with this staining } and no more post microtome staining. } } Good luck } } Teresa } } On 9/16/2020 6:43 PM, crwinkler-at-ncsu.edu wrote: } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hello all, } } } } We've recently moved to non-radioactive staining agents and are } } finding that these alternatives generally offer less contrast than } } their radioactive counterparts. In an effort to improve imaging of } } these lower contrast materials, I've tried to think of every major way } } to increase contrast from the TEM side of things. Our TEMs are } } materials 'scopes and we don't have access to a dedicated bio TEM, so } } I'm working with what I have. } } } } I've moved to lower voltages, from 200 to 80kV, used as small of an } } objective aperture as I can get away with, applied a generous amount } } of defocus during imaging, and used the GIF to form images from the } } elastic contribution. } } } } Am I missing anything major besides digital manipulation of } } brightness, contrast, and gamma on the images? Is it worth spending } } the time and effort to align at 20kV to improve contrast in thin } } sections? } } } } Thanks for any advice you can share, } } Chris } } } -- } Teresa Sawyer } Electron Microscopy Facility Instrument Manager } Oregon State University } Linus Pauling Science Center 145 } 541-737-5245 } www.science.oregonstate.edu/emfacility }
-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
The University bought 5 Zeiss Primo Star scopes for the Microbiology lab, and wanted them all with trinocular heads. Inexplicably, Zeiss shipped binocular heads with the scopes *and* the additional tinoculars. So we have 5 brand new binocular heads that Zeiss doesn't want back. Can anybody use them? The bean counters are willing to give them away (you pay shipping) but if there's some sort of trade or money involved, I'll look like a hero.
Not that I'm anticipating any takers. Most people want a trinoc as an accessory, not the other way around.
Cheers,
Jim
--
James M. Ehrman Digital Microscopy Facility Mount Allison University 63B York St. Sackville, NB E4L 1G7 CANADA
STEM is definitely the next item on the menu. The trick about inverting the HAADF image is a good one if you don't have a BF detector!
I'm all too familiar with samples blowing up under the beam. A thin coating of carbon on the exit surface (or sometimes both sides, in my experience) usually solves it as you indicate. If only the carbon coater was in the same building as the TEM...
Thanks for the advice, Chris
On Thu, Sep 17, 2020 at 11:18 AM Colijn, Hendrik {colijn.1-at-osu.edu} wrote: } } Hi Chris, } } HAADF STEM will definitely allow you to increase contrast. However, most of the bio people choke when looking at a DF image. Since the bio samples have basically mass-thickness contrast, you should be able to invert the contrast and directly compare it with a std BF TEM image. You can play with the camera length to adjust the image. } } In STEM, the samples may start charging or blowing up if not carbon coated since the local charge buildup in the STEM probe can cause issues. A few nm of carbon takes care of the problem. } } Good luck, } Henk } } ---------------- } } } Hendrik O. Colijn } Center for Electron Microscopy and AnalysiS } The Ohio State University } 1305 Kinnear Rd, Suite 100 } } colijn.1-at-osu.edu 614/643-3458 } cemas.osu.edu } } } -----Original Message----- } From: crwinkler-at-ncsu.edu {crwinkler-at-ncsu.edu} } Sent: Thursday, September 17, 2020 11:00 AM } To: Colijn, Hendrik {colijn.1-at-osu.edu} } Subject: [Microscopy] Re: Contrast enhancement strategies in TEM? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- https://urldefense.com/v3/__http://www.microscopy.com/MicroscopyListserver__;!!KGKeukY!igEIGfhUJ9v2_MZ1e8L5d_s2y_NT-NIeK6NUkt6sBSSFUj_jbiqukf6zvQVwexvM$ } On-Line Help https://urldefense.com/v3/__http://www.microscopy.com/MicroscopyListserver/FAQ.html__;!!KGKeukY!igEIGfhUJ9v2_MZ1e8L5d_s2y_NT-NIeK6NUkt6sBSSFUj_jbiqukf6zvYNZCtsY$ } ---------------------------------------------------------------------------- } } Hello Teresa, } } Thank you for the paper and the protocol. I'll pass it along to the scientist in charge to sample prep. } } The idea about switching to STEM in the SEM is quite interesting. } Thank you! That's definitely something we will test out. } } Cheers, } Chris } } } On Thu, Sep 17, 2020 at 10:26 AM Sawyer, Teresa {sawyerte-at-science.oregonstate.edu} wrote: } } } } Hello Chris, } } } } OSU also only has an analytical TEM and it is difficult to image } } biologicals. Luck for me I can do STEM imaging on one of our SEM. } } With a larger field of view and the extra staining I do it works out great. } } } } I was introduced to O-T-O-T-O staining a few years back and now I use } } it all the time. You might not need to do all three osmium staining } } but this method has eliminated the UA or alternative from the protocol. } } } } I have attached the protocol but I have modified it some over the years. } } I cut the osmium staining time in half. } } } } I can not stress enough how much easier my life is with this staining } } and no more post microtome staining. } } } } Good luck } } } } Teresa } } } } On 9/16/2020 6:43 PM, crwinkler-at-ncsu.edu wrote: } } } -------------------------------------------------------------------- } } } -------- The Microscopy ListServer -- CoSponsor: The Microscopy } } } Society of America To Subscribe/Unsubscribe -- } } } https://urldefense.com/v3/__http://www.microscopy.com/MicroscopyList } } } server__;!!KGKeukY!igEIGfhUJ9v2_MZ1e8L5d_s2y_NT-NIeK6NUkt6sBSSFUj_jb } } } iqukf6zvQVwexvM$ On-Line Help } } } https://urldefense.com/v3/__http://www.microscopy.com/MicroscopyList } } } server/FAQ.html__;!!KGKeukY!igEIGfhUJ9v2_MZ1e8L5d_s2y_NT-NIeK6NUkt6s } } } BSSFUj_jbiqukf6zvYNZCtsY$ } } } -------------------------------------------------------------------- } } } -------- } } } } } } Hello all, } } } } } } We've recently moved to non-radioactive staining agents and are } } } finding that these alternatives generally offer less contrast than } } } their radioactive counterparts. In an effort to improve imaging of } } } these lower contrast materials, I've tried to think of every major } } } way to increase contrast from the TEM side of things. Our TEMs are } } } materials 'scopes and we don't have access to a dedicated bio TEM, } } } so I'm working with what I have. } } } } } } I've moved to lower voltages, from 200 to 80kV, used as small of an } } } objective aperture as I can get away with, applied a generous amount } } } of defocus during imaging, and used the GIF to form images from the } } } elastic contribution. } } } } } } Am I missing anything major besides digital manipulation of } } } brightness, contrast, and gamma on the images? Is it worth spending } } } the time and effort to align at 20kV to improve contrast in thin } } } sections? } } } } } } Thanks for any advice you can share, Chris } } } } } -- } } Teresa Sawyer } } Electron Microscopy Facility Instrument Manager Oregon State } } University Linus Pauling Science Center 145 } } 541-737-5245 } } https://urldefense.com/v3/__http://www.science.oregonstate.edu/emfacil } } ity__;!!KGKeukY!igEIGfhUJ9v2_MZ1e8L5d_s2y_NT-NIeK6NUkt6sBSSFUj_jbiqukf } } 6zve2d9eoM$ } } } } } -- } Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://urldefense.com/v3/__https://www.aif.ncsu.edu/__;!!KGKeukY!igEIGfhUJ9v2_MZ1e8L5d_s2y_NT-NIeK6NUkt6sBSSFUj_jbiqukf6zvbKZeX3X$ } Cell: 267-496-0587 } } ==============================Original Headers============================== } 8, 55 -- From crwinkler-at-ncsu.edu Thu Sep 17 09:57:11 2020 8, 55 -- Received: from mail-il1-f169.google.com (mail-il1-f169.google.com [209.85.166.169]) } 8, 55 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 08HEvACD003430 } 8, 55 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2020 09:57:10 -0500 } 8, 55 -- Received: by mail-il1-f169.google.com with SMTP id a19so2583318ilq.10 } 8, 55 -- for {Microscopy-at-microscopy.com} ; Thu, 17 Sep 2020 08:06:50 -0700 (PDT) } 8, 55 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 55 -- d=ncsu.edu; s=google; } 8, 55 -- h=mime-version:references:in-reply-to:from:date:message-id:subject:to } 8, 55 -- :cc; } 8, 55 -- bh=4qZmelpKDRCs/tRW76F128muCjb4iTgn12RCfqNuZh0=; } 8, 55 -- b=IacG9CAWJgXnkcdIkVaZ8wlYEc8bIu8Y1hkuQ9rF8QDsVzJ+qaDEfdaUQLZBWFXMFm } 8, 55 -- dde+YE9DAHpvk77xEfEQyolWK88COEXLfU/xgMBc9epo6SZtemnZQBWuzDmI9Yflodbu } 8, 55 -- 4xYXVKjgCT9qSkI12Gxv2mPFi67fznQHwEwMIbeD8l1r6x5Lk/NKiYFz0kf7aAI0b6UM } 8, 55 -- 9MuHsSHIdeSM/aeadg/8aRkW2kyZpk6RQ9QpNSMz5jqOcgwz5cnLexeq5PuP+dYj+VOK } 8, 55 -- 6bkGpWQuOij2sYmes7aHjDm6M4Ee5+9VV1mccK7FTV7Y7Qn+r7cTN8BClpy8dTHq05qw } 8, 55 -- +0gw== } 8, 55 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 55 -- d=1e100.net; s=20161025; } 8, 55 -- h=x-gm-message-state:mime-version:references:in-reply-to:from:date } 8, 55 -- :message-id:subject:to:cc; } 8, 55 -- bh=4qZmelpKDRCs/tRW76F128muCjb4iTgn12RCfqNuZh0=; } 8, 55 -- b=uLzTYBZ5btQaknyXL1OkKu5DSebGlkU75sSRhKU5YLUIf8lEotzj50K/zZJy70FIpb } 8, 55 -- Wq+G6xk2/6JEPVgIC6i/Q8Y8w4bLwrXqsURhetbAA0G5f8B5XAExHOcrTBQUQXm9ylSX } 8, 55 -- O3F/DF5GI1D5SYsPY8+I2EOvOYq1zoAal4Fst56wOT0MuOGqBqts/VjRAH7T72wswxog } 8, 55 -- OOwzq/8kRJFiKPHUp/DKowR29u9B5jrRwR9RKlm5i3xMN93shJLLhZYzn6EFV3IniBuD } 8, 55 -- Jdw2w+7DrpbVH9qk/ZapFU3KUQNBZ1gRU3cfbfVr7c0f1gs7UYkiZhjvQe2X9FsUm1+w } 8, 55 -- BzZw== } 8, 55 -- X-Gm-Message-State: AOAM533elxoeg9WJN8LB58jtsNYRwCNqU3JVsUw88X59qc7rIN6Vg4Je } 8, 55 -- Zf8t+RBM056T/L3PTAKyJaDFi++6l5HfIK/E1zvHlvkDY7gewj+eBB4xl5w1HNBeCjTNc1wTOkf } 8, 55 -- SWckzf+KxKZArrXFEByz1LC8zPhdEY603otQamFEDyareEWStyFiyonQu2Rt3PtW6MmmXsWo= } 8, 55 -- X-Google-Smtp-Source: ABdhPJwrsL1cHhh2vuaSfLn1OXjoWlcj/R9A1aDD1oXZ4n/dkBzQVUQdF/BtAH3Pxsm59/fu9BVfUQ== } 8, 55 -- X-Received: by 2002:a92:cb11:: with SMTP id s17mr2188065ilo.218.1600355209312; } 8, 55 -- Thu, 17 Sep 2020 08:06:49 -0700 (PDT) } 8, 55 -- Received: from mail-il1-f180.google.com (mail-il1-f180.google.com. 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-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
I'm not sure about the kind of microscope Phil was referring to, but as for FEI microscopes such as Tecnai the diffraction plane is at the selected aperture plane *only when in low mag mode (objective lens off)*. So I guess in Phil's TEM it might be something similar.
Chris, I have used JEOL TEM a few times, so I am not quite familiar with. But, yes there might be a way of switching off the objective less liki in low magnification mode. You will get a better contrast.
Another possibility that might work, as pointed out by Henk is to use the ADF or HAADF detector to do dark field imaging and invert the image contrast. I've done (inverted contrast) ADF imaging on non stained tissues and it works (with that sample I could see absolutely nothing in TEM mode).
Another thing you can think of is to do BSE-SEM imaging of the sectioned embedded block's surface. Of course you need to carbon coat its surface before. Use the right SEM settings to reduce the interaction volume. Maybe you can try lower kV, lower WD.
Good luck,
Erico
Em qui., 17 de set. de 2020 às 10:45, {colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} } escreveu:
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Hi Phil,
I guess I don't understand how the SA aperture increases contrast since it is in an image plane not a diffraction plane. You are referring to the SA aperture, right?
As a note... GIF is "Gatan Imaging Filter". Chris is trying to improve contrast by energy-filtering.
Cheers, Henk
----------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
-----Original Message----- X-from: oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } Sent: Thursday, September 17, 2020 8:03 AM To: Colijn, Hendrik {colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} } Subject: [Microscopy] Re: [External] Contrast enhancement strategies in TEM?
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I'm not sure what you mean by "GIF" in this case (I'm assuming you don't mean the file format), but if this isn't an aperture ... since you have materials 'scopes, you have a diffraction/field of view aperture. You can use this aperture to increase the contrast without any resolution loss (since this aperture isn't in the objective lens). It will give you a bit of resolution improvement by removing peripheral electrons, but the main negative effect is the vignetting. On our TEM, we can't use even the largest (200 µm) below ~10kX. The contrast increase is less than that from using the objective aperture, but it's noticeable.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office
-----Original Message----- X-from: "crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} " {crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} } Reply-To: "crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} " {crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} } Date: Wednesday, 16September, 2020 at 21:57 To: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } Subject: [External] [Microscopy] Contrast enhancement strategies in TEM?
Hello all,
We've recently moved to non-radioactive staining agents and are finding that these alternatives generally offer less contrast than their radioactive counterparts. In an effort to improve imaging of these lower contrast materials, I've tried to think of every major way to increase contrast from the TEM side of things. Our TEMs are materials 'scopes and we don't have access to a dedicated bio TEM, so I'm working with what I have.
I've moved to lower voltages, from 200 to 80kV, used as small of an objective aperture as I can get away with, applied a generous amount of defocus during imaging, and used the GIF to form images from the elastic contribution.
Am I missing anything major besides digital manipulation of brightness, contrast, and gamma on the images? Is it worth spending the time and effort to align at 20kV to improve contrast in thin sections?
Thanks for any advice you can share, Chris
-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University
It is a FEI/Thermofhisher machine you could try imaging your samples at low magnification (LM) mode. In LM the objective lenses are off (you will see the value around 6% of it strength), and in that case the diffraction lenses act out as the objective lens (you may even want to place the selected area aperture. In FEI machines you will have a better contrast in LM mode. I know the magnification is not that high in LM mode, but you may still benefit from using your GIF camera that will give you an extra magnification.
Best wishes
Em qua., 16 de set. de 2020 às 22:57, {crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} } escreveu:
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Hello all,
We've recently moved to non-radioactive staining agents and are finding that these alternatives generally offer less contrast than their radioactive counterparts. In an effort to improve imaging of these lower contrast materials, I've tried to think of every major way to increase contrast from the TEM side of things. Our TEMs are materials 'scopes and we don't have access to a dedicated bio TEM, so I'm working with what I have.
I've moved to lower voltages, from 200 to 80kV, used as small of an objective aperture as I can get away with, applied a generous amount of defocus during imaging, and used the GIF to form images from the elastic contribution.
Am I missing anything major besides digital manipulation of brightness, contrast, and gamma on the images? Is it worth spending the time and effort to align at 20kV to improve contrast in thin sections?
Thanks for any advice you can share, Chris
-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
Physicist / Microscopist / Lab Manager Transmission Electron Microscopy for Materials Science laboratory Center of Microscopy Universidade Federal de Minas Gerais (UFMG) Av. Antônio Carlos, 6627, Campus Pampulha, Belo Horizonte, MG, Brazil. ZIP Code 31270-901. +55-31-3409-7573 +55-31-3409-7575
Hi Henk and Erico, I can confirm BSE-SEM imaging can really be advantageous even on ultrathin sections. A thin carbon coating is a must, as Erico mentioned in his message. In BSE imaging mode one can play with accelerating voltage, concentric back-scattered detector rings and beam deceleration setting. With an optimal setting, you can image the really huge section on TEM grid with almost no interference of TEM grid bars. Some years ago we used such approaches on imaging of the whole ultrathin sections through mice tooth. We were searching for places with Tomes’ processes in ameloblasts and we were successful.
Regards Oldrich
-- Oldřich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group Vídeňská 1083 142 20 Prague 4 Czech Republic
On Thu, 17 Sep 2020 17:48:37 -0500, microscopy.listserver-at-gmail.com wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Erico Freitas {ericotadeu-at-ufmg.br} } } } } Hi Henk, } } I'm not sure about the kind of microscope Phil was referring to, but } as for FEI microscopes such as Tecnai the diffraction plane is at the } selected aperture plane *only when in low mag mode (objective lens } off)*. So I guess in Phil's TEM it might be something similar. } } Chris, I have used JEOL TEM a few times, so I am not quite familiar } with. But, yes there might be a way of switching off the objective } less liki in low magnification mode. You will get a better contrast. } } Another possibility that might work, as pointed out by Henk is to use } the ADF or HAADF detector to do dark field imaging and invert the } image contrast. I've done (inverted contrast) ADF imaging on non } stained tissues and it works (with that sample I could see absolutely } nothing in TEM mode). } } Another thing you can think of is to do BSE-SEM imaging of the } sectioned embedded block's surface. Of course you need to carbon coat } its surface before. Use the right SEM settings to reduce the } interaction volume. Maybe you can try lower kV, lower WD. } } Good luck, } } Erico } } } } } } Em qui., 17 de set. de 2020 às 10:45, {colijn.1-at-osu.edu } {mailto:colijn.1-at-osu.edu} } escreveu: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line } {http://www.microscopy.com/MicroscopyListserverOn-Line} Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi Phil, } } I guess I don't understand how the SA aperture increases } contrast since it is in an image plane not a diffraction plane. You } are referring to the SA aperture, right? } } As a note... GIF is "Gatan Imaging Filter". Chris is trying to } improve contrast by energy-filtering. } } Cheers, } Henk } } ---------------- } } } Hendrik O. Colijn } Center for Electron Microscopy and AnalysiS } The Ohio State University } 1305 Kinnear Rd, Suite 100 } } colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} 614/643-3458 } cemas.osu.edu {http://cemas.osu.edu} } } } -----Original Message----- } X-from: oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } } Sent: Thursday, September 17, 2020 8:03 AM } To: Colijn, Hendrik {colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} } } Subject: [Microscopy] Re: [External] Contrast enhancement } strategies in TEM? } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society } of America To Subscribe/Unsubscribe -- } } https://urldefense.com/v3/__http://www.microscopy.com/MicroscopyListserver__;!!KGKeukY!jFW1hv1KxTkxRas5T101-Yd5jP024ydHnCLl-ddxiARaJ84iUFKXQOAQqVpOhTuK$ } On-Line } } {https://urldefense.com/v3/__http://www.microscopy.com/MicroscopyListserver__;!!KGKeukY!jFW1hv1KxTkxRas5T101-Yd5jP024ydHnCLl-ddxiARaJ84iUFKXQOAQqVpOhTuK$On-Line} } Help } } https://urldefense.com/v3/__http://www.microscopy.com/MicroscopyListserver/FAQ.html__;!!KGKeukY!jFW1hv1KxTkxRas5T101-Yd5jP024ydHnCLl-ddxiARaJ84iUFKXQOAQqVyx_TAW$ } ---------------------------------------------------------------------------- } } Chris, } } I'm not sure what you mean by "GIF" in this case (I'm assuming } you don't mean the file format), but if this isn't an aperture ... } since you have materials 'scopes, you have a diffraction/field of } view aperture. You can use this aperture to increase the contrast } without any resolution loss (since this aperture isn't in the } objective lens). It will give you a bit of resolution improvement by } removing peripheral electrons, but the main negative effect is the } vignetting. On our TEM, we can't use even the largest (200 µm) below } ~10kX. The contrast increase is less than that from using the } objective aperture, but it's noticeable. } } Phil } ------------- } Philip Oshel } Imaging Facility Director } Biology Department } 1304 Biosciences } 1455 Calumet Ct. } Central Michigan University } Mt. Pleasant, MI 48859 } 989 774-3576 office } } -----Original Message----- } X-from: "crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} " } {crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} } } Reply-To: "crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} " } {crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} } } Date: Wednesday, 16September, 2020 at 21:57 } To: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu } {mailto:oshel1pe-at-cmich.edu} } Subject: [External] [Microscopy] } Contrast enhancement strategies in TEM? } } Hello all, } } We've recently moved to non-radioactive staining agents and } are finding that these alternatives generally offer less contrast than } their radioactive counterparts. In an effort to improve } imaging of these lower contrast materials, I've tried to think of } every major way to increase contrast from the TEM side of things. Our } TEMs are materials 'scopes and we don't have access to a dedicated } bio TEM, so I'm working with what I have. } } I've moved to lower voltages, from 200 to 80kV, used as } small of an objective aperture as I can get away with, applied a } generous amount of defocus during imaging, and used the GIF to form } images from the elastic contribution. } } Am I missing anything major besides digital manipulation of } brightness, contrast, and gamma on the images? Is it worth } spending the time and effort to align at 20kV to improve contrast in } thin sections? } } Thanks for any advice you can share, } Chris } } -- } Transmission Electron Microscopy Lab Manager } Analytical Instrumentation Facility (AIF) } NC State University } } https://urldefense.com/v3/__https://www.aif.ncsu.edu/__;!!KGKeukY!jFW1hv1KxTkxRas5T101-Yd5jP024ydHnCLl-ddxiARaJ84iUFKXQOAQqf0ottp3$ } } Cell: 267-496-0587 } } } } ==============================Original } Headers============================== 23, 140 -- From } colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} Thu Sep 17 08:30:57 2020 } 23, 140 -- Received: from mx0b-002cfd01.pphosted.com } {http://mx0b-002cfd01.pphosted.com} (mx0b-002cfd01.pphosted.com } {http://mx0b-002cfd01.pphosted.com} [148.163.155.97]) 23, 140 -- } by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) } with ESMTP id 08HDUu09006734 23, 140 -- for } {Microscopy-at-Microscopy.com} ; Thu, 17 Sep 2020 08:30:57 -0500 23, 140 } -- Received: from pps.filterd (m0130879.ppops.net } {http://m0130879.ppops.net} [127.0.0.1]) 23, 140 -- by } mx0a-002cfd01.pphosted.com {http://mx0a-002cfd01.pphosted.com} } (8.16.0.42/8.16.0.42 {http://8.16.0.42/8.16.0.42} ) with SMTP id } 08HDZj6P019984; 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==============================Original Headers============================== 9, 29 -- From benada-at-biomed.cas.cz Fri Sep 18 01:31:55 2020 9, 29 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 9, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 08I6VsMC003456 9, 29 -- for {microscopy-at-microscopy.com} ; Fri, 18 Sep 2020 01:31:55 -0500 9, 29 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 9, 29 -- (using TLSv1.3 with cipher TLS_AES_256_GCM_SHA384 (256/256 bits) 9, 29 -- key-exchange ECDHE (P-256) server-signature RSA-PSS (2048 bits) server-digest SHA256) 9, 29 -- (No client certificate requested) 9, 29 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 2CAAED00F9A 9, 29 -- for {microscopy-at-microscopy.com} ; Fri, 18 Sep 2020 08:41:32 +0200 (CEST) 9, 29 -- Date: Fri, 18 Sep 2020 08:41:31 +0200 9, 29 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 9, 29 -- To: {microscopy-at-microscopy.com} 9, 29 -- Subject: Re: [Microscopy] Fwd: Re: [External] Contrast enhancement 9, 29 -- strategies in 9, 29 -- Message-ID: {20200918084131.23b974f8-at-u117ob02} 9, 29 -- In-Reply-To: {202009172248.08HMmbCR025198-at-microscopy.com} 9, 29 -- References: {202009172248.08HMmbCR025198-at-microscopy.com} 9, 29 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 9, 29 -- =?UTF-8?B?xIxS?= 9, 29 -- X-Mailer: Claws Mail 3.17.3 (GTK+ 2.24.32; i686-pc-linux-gnu) 9, 29 -- MIME-Version: 1.0 9, 29 -- Content-Type: text/plain; charset=UTF-8 9, 29 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 9, 29 -- X-IoP-CAS-MailScanner-ID: 2CAAED00F9A.ACB3C 9, 29 -- X-IoP-CAS-MailScanner: Processed 9, 29 -- X-Spam-Status: No 9, 29 -- Content-Transfer-Encoding: 8bit 9, 29 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 08I6VsMC003456 ==============================End of - Headers==============================
Thank you to all for your responses and advice! I want to summarize the responses I received regarding my query on how to improve contrast on low contrast samples in a materials science TEM:
1) Buy a Bio TEM with a large gap objective optimized for high contrast. =D
2) Adjust staining protocols--check pH levels, switch to radioactive stains (EH&S now makes this difficult in some universities), supplement non-radioactive stains with lead citrate, tannic acid, or other agents, and so forth. One responder linked me to the following protocol that worked wonders for her: https://www.sciencedirect.com/science/article/pii/S1047847714002378
3) Try to minimize contribution from the grid by moving to ultrathin carbon or plasma cleaning thicker carbon to thin it down.
4) Acquire EFTEM SI and generate jump-ratio maps around the plasmons peak, carbon peaks, etc.
5) Switch to STEM and see if contrast is improved when using ADF or BF detectors. If you only have an ADF detector, invert the image contrast to give a pseudo BF image (more acceptable to people used to TEM). Similarly, switch to the SEM and try STEM or BSE imaging at 30kV.
6) Send a sample to Delong instruments and see how it looks at 5, 15, and/or 25kV. Also, try working at lower voltages like 40 or 60kV.
7) Work in low mag (LM) and use the selected area aperture to further improve contrast. LM contrast will be inherently higher as the objective lens is turned nearly off.
8) Sum/stack several images to improve SNR. For thin sections with little contrast and no outstanding features for correlation, this will be tricky.
9) Replace one of the objective apertures with a phase plate.
10) For JEOL and Hitachi microscopes, use the field limiting aperture located below the objective to improve contrast.
Thanks again to everyone for all the help. We'll be trying STEM in the TEM and SEM next.
Thanks, Chris
-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
I have a Hitachi 7700. The FOV (field-of-view), aka selected area aperture, works at all mags, not just in low mag. I suspect the same is true for any TEM with a SA (selected area - let's expand the abbreviations in the emails). Just do your imaging as normal, and if you need a contrast bump without losing resolution, put in the SA/FOV aperture. It's just adding another aperture, not changing any imaging parameters or any electronics. You do lose field of view at mags {10,000 or so, depending on the aperture size, but that's it. Assuming you have a manually variable aperture and not some computer-controlled thing that only works when and how the computer thinks it should. If that doesn't exist, it's coming.
Phil ----------------------------------------- Philip Oshel Imaging Center Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 office (989) 774-7567 lab
________________________________________ X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, September 17, 2020 18:54 To: Oshel, Philip Eugene
X-from: Erico Freitas {ericotadeu-at-ufmg.br}
Hi Henk,
I'm not sure about the kind of microscope Phil was referring to, but as for FEI microscopes such as Tecnai the diffraction plane is at the selected aperture plane *only when in low mag mode (objective lens off)*. So I guess in Phil's TEM it might be something similar.
Chris, I have used JEOL TEM a few times, so I am not quite familiar with. But, yes there might be a way of switching off the objective less liki in low magnification mode. You will get a better contrast.
Another possibility that might work, as pointed out by Henk is to use the ADF or HAADF detector to do dark field imaging and invert the image contrast. I've done (inverted contrast) ADF imaging on non stained tissues and it works (with that sample I could see absolutely nothing in TEM mode).
Another thing you can think of is to do BSE-SEM imaging of the sectioned embedded block's surface. Of course you need to carbon coat its surface before. Use the right SEM settings to reduce the interaction volume. Maybe you can try lower kV, lower WD.
Good luck,
Erico
Em qui., 17 de set. de 2020 às 10:45, {colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} } escreveu:
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Hi Phil,
I guess I don't understand how the SA aperture increases contrast since it is in an image plane not a diffraction plane. You are referring to the SA aperture, right?
As a note... GIF is "Gatan Imaging Filter". Chris is trying to improve contrast by energy-filtering.
Cheers, Henk
----------------
Hendrik O. Colijn Center for Electron Microscopy and AnalysiS The Ohio State University 1305 Kinnear Rd, Suite 100
-----Original Message----- X-from: oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } Sent: Thursday, September 17, 2020 8:03 AM To: Colijn, Hendrik {colijn.1-at-osu.edu {mailto:colijn.1-at-osu.edu} } Subject: [Microscopy] Re: [External] Contrast enhancement strategies in TEM?
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I'm not sure what you mean by "GIF" in this case (I'm assuming you don't mean the file format), but if this isn't an aperture ... since you have materials 'scopes, you have a diffraction/field of view aperture. You can use this aperture to increase the contrast without any resolution loss (since this aperture isn't in the objective lens). It will give you a bit of resolution improvement by removing peripheral electrons, but the main negative effect is the vignetting. On our TEM, we can't use even the largest (200 µm) below ~10kX. The contrast increase is less than that from using the objective aperture, but it's noticeable.
Phil ------------- Philip Oshel Imaging Facility Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 989 774-3576 office
-----Original Message----- X-from: "crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} " {crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} } Reply-To: "crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} " {crwinkler-at-ncsu.edu {mailto:crwinkler-at-ncsu.edu} } Date: Wednesday, 16September, 2020 at 21:57 To: "Oshel, Philip Eugene" {oshel1pe-at-cmich.edu {mailto:oshel1pe-at-cmich.edu} } Subject: [External] [Microscopy] Contrast enhancement strategies in TEM?
Hello all,
We've recently moved to non-radioactive staining agents and are finding that these alternatives generally offer less contrast than their radioactive counterparts. In an effort to improve imaging of these lower contrast materials, I've tried to think of every major way to increase contrast from the TEM side of things. Our TEMs are materials 'scopes and we don't have access to a dedicated bio TEM, so I'm working with what I have.
I've moved to lower voltages, from 200 to 80kV, used as small of an objective aperture as I can get away with, applied a generous amount of defocus during imaging, and used the GIF to form images from the elastic contribution.
Am I missing anything major besides digital manipulation of brightness, contrast, and gamma on the images? Is it worth spending the time and effort to align at 20kV to improve contrast in thin sections?
Thanks for any advice you can share, Chris
-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University
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Title-Subject: [Filtered] TEM fungi
Message: Does anyone have a protocol for embedding fungi? thanks sue
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Any specific fungal structures you are targeting? Hyphae, conidia, yeast? Conventional fixation or cryo?
Best Regards, Kirk
Kirk J Czymmek, Ph.D. Director, Advanced Bioimaging Laboratory Principal Investigator Donald Danforth Plant Science Center 975 North Warson Road|Saint Louis, Missouri 63132 t:314.587.1261 | c:203.833.3476 |f:314.587.1503 | e:kczymmek-at-danforthcenter.org www.danforthcenter.org
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Saturday, September 19, 2020 4:14 PM To: Czymmek, Kirk {KCzymmek-at-danforthcenter.org}
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Hi, Sue You may want to contact the CICESE branch of the National Laboratory of Advanced Microscopy https://www.facebook.com/pg/LNMA.CICESE/about/ They are very skilled in EM of fungi. Cheers Vad
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X-from: Susan Van Horn {susan.vanhorn-at-stonybrook.edu}
Hi - hyphae and normal fixation........thanks for quick reply sue
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Any specific fungal structures you are targeting? Hyphae, conidia, yeast? Conventional fixation or cryo?
Best Regards, Kirk
Kirk J Czymmek, Ph.D. Director, Advanced Bioimaging Laboratory Principal Investigator Donald Danforth Plant Science Center 975 North Warson Road|Saint Louis, Missouri 63132 t:314.587.1261 | c:203.833.3476 |f:314.587.1503 | e:kczymmek-at-danforthcenter.org {mailto:e%3Akczymmek-at-danforthcenter.org} www.danforthcenter.org {http://www.danforthcenter.org}
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*************************************************************** Susan C. Van Horn, M.A. C-MIC TEM Facility Office of Scientific Affairs Life Sciences Building Room 046 SUNY-at-Stony Brook Stony Brook, NY 11794-5200 phone: 631-632-8623 fax: 631-632-7728 email: susan.vanhorn-at-stonybrook.edu {mailto:susan.vanhorn-at-sunysb.edu} Central Microscopy Imaging Center (C-MIC) website: https://osa.stonybrookmedicine.edu/research-core-facilities/microscopy/services
Hi Sue, While Embed812 and similar epoxy resins can work, in general I like to use graded acetone series and Quetol 651 for fungi (and plants) as it has good contrast and its low viscosity help reduce the possibility of infiltration problems due to the fungal cell wall.
I will reach out to you directly with more detailed protocol.
Best Regards, Kirk
Kirk J Czymmek, Ph.D. Director, Advanced Bioimaging Laboratory Principal Investigator Donald Danforth Plant Science Center 975 North Warson Road|Saint Louis, Missouri 63132 t:314.587.1261 | c:203.833.3476 |f:314.587.1503 | e:kczymmek-at-danforthcenter.org www.danforthcenter.org
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Monday, September 21, 2020 7:42 AM To: Czymmek, Kirk {KCzymmek-at-danforthcenter.org}
X-from: Susan Van Horn {susan.vanhorn-at-stonybrook.edu}
Hi - hyphae and normal fixation........thanks for quick reply sue
On Sun, Sep 20, 2020 at 9:25 AM {microscopy.listserver-at-gmail.com
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Any specific fungal structures you are targeting? Hyphae, conidia, yeast? Conventional fixation or cryo?
Best Regards, Kirk
Kirk J Czymmek, Ph.D. Director, Advanced Bioimaging Laboratory Principal Investigator Donald Danforth Plant Science Center 975 North Warson Road|Saint Louis, Missouri 63132 t:314.587.1261 | c:203.833.3476 |f:314.587.1503 | e:kczymmek-at-danforthcenter.org {mailto:e%3Akczymmek-at-danforthcenter.org} www.danforthcenter.org {http://www.danforthcenter.org}
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Email: susan.vanhorn-at-stonybrook.edu {mailto:susan.vanhorn-at-stonybrook.edu} Name: Susan C Van Horn
Title-Subject: [Filtered] TEM fungi
Message: Does anyone have a protocol for embedding fungi? thanks sue
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*************************************************************** Susan C. Van Horn, M.A. C-MIC TEM Facility Office of Scientific Affairs Life Sciences Building Room 046 SUNY-at-Stony Brook Stony Brook, NY 11794-5200 phone: 631-632-8623 fax: 631-632-7728 email: susan.vanhorn-at-stonybrook.edu {mailto:susan.vanhorn-at-sunysb.edu} Central Microscopy Imaging Center (C-MIC) website: https://osa.stonybrookmedicine.edu/research-core-facilities/microscopy/services
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Email: eric.gautron-at-cnrs-imn.fr Name: Eric Gautron
Title-Subject: [Filtered] Flucam saturation on Themis Z
Message: Hi all ThemisZ users, since the upgrade of the main TEM software to 2.15.3, the flucam saturates and the monochromator stigmators tuning with Optimono fails (probably due to that saturation). Despite remote and on-site support and a flucam exchange, the situation is still the same than two months ago. Does anybody have the same problem ? Best regards, Eric
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Email: cni-at-udel.edu Name: Chaoying Ni
Title-Subject: [Filtered] Immediate job opening - research associate
Message: University of Delaware has an immediate opening for a post-doctoral research associate ideally with any of TEM/SEM/AFM experience in physical science. The successful candidate may become a permanent staff down the load as the campus life becomes more normal. For more information and application, please check out the site below:
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Title-Subject: [Filtered] TEM Research Associate position at NIH
Message: Dear colleagues,
We have an open Research Associate position at the Frederick National Laboratory for Cancer Research in Frederick, Maryland. The primary responsibilities will be to prepare samples (mostly proteins) for EM imaging by negative staining and plunge freezing and to collect data using a range of electron microscopes, with opportunities to perform other tasks such as computational processing. Traditional EM (plastic embedding/sectioning) will be performed on occasion. This position requires a bachelors degree. Proficiency in the use of transmission electron microscopes and desire to learn will be of high value. Please use the following link to see the details and apply: https://leidosbiomed.csod.com/ats/careersite/jobdetails.aspx?site=4&c=leidosbiomed&id=1380
Yaroslav Tsybovsky, Ph.D. (contractor) Scientist II Frederick National Laboratory for Cancer Research Leidos Biomedical Research, Inc. Post Office Box B Frederick, Maryland 21702 Phone: 301-846-7550
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Email: sobol-at-img.cas.cz Name: Margarita Sobol
Title-Subject: [Filtered] the software script allowing the automatic focusing of the samples during the EM high-throughput acquisition
Message: I use Jeol 1400 Flash EM, which has a montage system using the stage drive to shift the field of view (Limitless Panorama). This system enables a montage panorama image to be captured over a wide area. Actually, I acquire the grid squares one by one and I always focus each grid square separately. Also I check if the focus is OK for each corner and the center of the grid square. Usually, it is so when the acquisition starts, but after the first hour (LLP lasts 6-8 hours for the grid square at x30K) the image becomes to be out of focus and I need to pause the capturing and adjust the focus manually. This means that the overnight LLP is often useless for the analysis. I need the software script which allows the automatic focusing of the samples during the EM high-throughput acquisition Thanks a lot in advance!
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Email: sayitugurlu-at-gmail.com Name: Sayit Uğurlu
Title-Subject: [Filtered] New type of EDS detector
Message: I have an idea about creating a new type of x-ray detector for scanning electron microscopy. Unfortunately, I cannot give much information yet but here is some part I can share without rewiring how the detector works. The detector itself is actually not very complex. It is based on some foundementers physic laws. I am expecting that it would have some unique properties like detecting very low traces of elements and detecting soft x-ray and UV.It also would be a new technology so I am hoping that It would be patent-able. So I am just asking 45 min. to listen to me,If there anyone willingly investigate it. B.R. Sayit Uğurlu
Abstract: The purpose of this project is creating a new kind of detector for X-ray characterization. Each current technology has some level of disadvantage. The Windowless EDS has low resolution and hard to detect low energy x-ray. The WDS is expensive. This detector design for detecting very low energy x-ray and getting better resolution. Also, It would be the cheapest x-ray spectrometer in the market.
In theory it can; -detect Li and other soft X-ray -It is cheap. Like 7000-8000 euro total cost. -It is hard to say something about resolution. If you can wait you can detect very low % of elements Like %0,01.
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Email: marianne.mai-at-boeckeler.com Name: Marianne Mai
Title-Subject: [Filtered] Sales Manager USA Position
Message: Boeckeler Instruments is seeking a Sales Manager for the USA region to develop and manage customer and dealer relationships for our line of ultramicrotomy products and services. Key Responsibilities
Drive growth across sales channels (direct and through intermediaries) Develop and implement sales plans and forecasts to achieve corporate objectives for products and services Oversee and evaluate market research and adjust sales/marketing strategy to meet changing market and competitive conditions Establish and maintain relationships with industry influencers and key strategic partners Direct market channel development activity and coordinate sales distribution by establishing sales territories, quotas and goals Represent company at trade association meetings, workshops and other events Organize online or onsite workshops, demos and other customer related events Meet with key clients and intermediaries to maintain relationships, negotiate and close deals Install and train at customer sites when required Prepare periodic sales report showing sales volume, potential sales, and areas of proposed client base expansion Review and analyze sales performances against programs, quotes and plans to determine effectiveness Collaborate with Applications and R&D teams on market feedback for the development of new solutions Qualifications
Bachelors degree, Masters degree or PhD in Engineering, Biology or related field Minimum of three years or more in managing dealer and or end-user relationships Experience in engineering, life science or materials science research Experience in various microscopy techniques is highly desired Have excellent people skills Able to travel regularly Based in or willing to relocate to Tucson, AZ
Please send your CV to careers-at-boeckeler.com
About Boeckeler Instruments Boeckeler Instruments, Inc. is a privately-owned company that engineers sample preparation equipment for nanoscale research, under the RMC Boeckeler product line. Our solutions are employed in a broad range of areas including materials science and cell biology, with special emphasis in 3D sample preparation. Boeckeler Instruments, Inc. is an Equal Opportunity/Affirmative Action Employer.
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Haven't seen it used with SEMs yet, but (unless you find another off-the-shelf plug-and-play solution) I would suggest you to look at
https://micro-manager.org/
This open-source package ss often used for automating wide area acquisitions with optical microscopes, has built-in autofocus routine, and integrate-able with ImageJ and Matlab. If remote control protocol for your SEM is known then it very much could be adapted for your application.
Just thinking out loud - no connection with any of the mentioned SW packages or their manufacturers here...
Would also suggest to make a summary of the responses you get, as wide area image acquisition is of interest and information you gather could be beneficial to quite a few people .
Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 www.partbeamsystech.com www.fibsemproducts.com www.freudlabs.com
"Only the Paranoid Survive" (A.Grove & SpaceX QA)
On 9/25/2020 5:39 PM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: sobol-at-img.cas.cz } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } sobol-at-img.cas.cz, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: sobol-at-img.cas.cz Name: Margarita Sobol } } Title-Subject: [Filtered] the software script allowing the automatic focusing of the samples during } the EM high-throughput acquisition } } Message: I use Jeol 1400 Flash EM, which has a montage system using the stage drive to shift the } field of view (Limitless Panorama). This system enables a montage panorama image to be captured over } a wide area. Actually, I acquire the grid squares one by one and I always focus each grid square } separately. Also I check if the focus is OK for each corner and the center of the grid square. } Usually, it is so when the acquisition starts, but after the first hour (LLP lasts 6-8 hours for the } grid square at x30K) the image becomes to be out of focus and I need to pause the capturing and } adjust the focus manually. This means that the overnight LLP is often useless for the analysis. } I need the software script which allows the automatic focusing of the samples during the EM } high-throughput acquisition } Thanks a lot in advance! } } Login Host: 147.231.146.127 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 7, 54 -- From microscopy.listserver-at-gmail.com Fri Sep 25 16:39:32 2020 } 7, 54 -- Received: from mail-il1-f174.google.com (mail-il1-f174.google.com [209.85.166.174]) } 7, 54 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 08PLdVt6016125 } 7, 54 -- for {microscopy-at-microscopy.com} ; Fri, 25 Sep 2020 16:39:31 -0500 } 7, 54 -- Received: by mail-il1-f174.google.com with SMTP id z5so3759978ilq.5 } 7, 54 -- for {microscopy-at-microscopy.com} ; Fri, 25 Sep 2020 14:49:38 -0700 (PDT) } 7, 54 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 54 -- d=gmail.com; s=20161025; } 7, 54 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 7, 54 -- :in-reply-to:content-language:content-transfer-encoding; } 7, 54 -- bh=SLbua5UNzYSfsOMlD/FdD236PF/kOrPJ5vIG+00SJLg=; } 7, 54 -- b=t/OCA5fdNUPFjr41w4XYxE9xNm+pcYrxvj7/gLLE8i+0vyl38laIxz+CX/sCLolQyN } 7, 54 -- sjnA2nUNjlxADQBYmHW5EUy/1Hlzyvws7RRVfBROGz1zCgF+tFM4wjac3pflvqV75K+Y } 7, 54 -- HrSDtg89b4zezT70pwvNTg4g14wm0X8WD/akN9M1X1gwuqXB8pxrSfSv3fSVyNkpXger } 7, 54 -- qist5scQh9pQn20NwO06ZPKwj6ubUvY7nKvgIZp8RLwqfdMHvvIOqqFITanO/Ukm5TWM } 7, 54 -- AT/qzvsytnnDoMOs+rdm5tk3pEZkK/rDO0OCCFCIAbNNsVPqBCPny4nJsHOcCgekeRIG } 7, 54 -- o4XQ== } 7, 54 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 54 -- d=1e100.net; s=20161025; } 7, 54 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 7, 54 -- :user-agent:mime-version:in-reply-to:content-language } 7, 54 -- :content-transfer-encoding; } 7, 54 -- bh=SLbua5UNzYSfsOMlD/FdD236PF/kOrPJ5vIG+00SJLg=; } 7, 54 -- b=ENOae8UlW6Z7j1cwyiP6z5mbZfsL+NIyx0ci8uDYBFpgHywnGV+WFtDJdLqGMHz8JC } 7, 54 -- tDIkXi02xaPqj6n7r+pJpfoXjG2iwEdsOh58mZX+qfKMri8xzTakO25neZc/hCcWWUll } 7, 54 -- mM6ldVGa2nN0gkRUPx3V0eg7w4L3QRMKqS6MD+nFS+Dl5dQh1abot50M+e+pyuete6co } 7, 54 -- wf02xiZCWMrsrVN8AUjAHNXfDtjwLkvJMno8IuFNIfI7dbN5B2CC2aiSiO3fhufZiXjz } 7, 54 -- cglfF8MIcoLokYPfrDEHfOvQF2zC42wNlIMsrMPcFXL/Q7MOuyLfc99IjbpwNd8fH0F6 } 7, 54 -- zpLA== } 7, 54 -- X-Gm-Message-State: AOAM533IJ/11zY3mMDdQF5GJjVLnuBGMhLkOidzGgnXtTTSc7L36Ev8t } 7, 54 -- sZKhYr3/bT1o7X0H4KQ/PsqgifzfjEM= } 7, 54 -- X-Google-Smtp-Source: ABdhPJy+5L3r41e9QbIlPsxBRVqPB1FfJFMIoAUD05uMhM6tEj9SrY8/R1OviTK695TojE2E4mFUYQ== } 7, 54 -- X-Received: by 2002:a92:c301:: with SMTP id n1mr1737141ilg.247.1601070577277; } 7, 54 -- Fri, 25 Sep 2020 14:49:37 -0700 (PDT) } 7, 54 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:b1df:6382:4977:7799]) } 7, 54 -- by smtp.googlemail.com with ESMTPSA id i144sm1702436ioa.55.2020.09.25.14.49.36 } 7, 54 -- for {microscopy-at-microscopy.com} } 7, 54 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 7, 54 -- Fri, 25 Sep 2020 14:49:36 -0700 (PDT) } 7, 54 -- Subject: viaWWW:software script allowing the automatic focusing of the samples } 7, 54 -- during the EM high-throughput acquisition } 7, 54 -- References: {202009251532.08PFWd4E028530-at-microscopy.com} } 7, 54 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 7, 54 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 7, 54 -- X-Forwarded-Message-Id: {202009251532.08PFWd4E028530-at-microscopy.com} } 7, 54 -- Message-ID: {19e6cfe4-bd42-ea56-8562-ed95e03be34e-at-gmail.com} } 7, 54 -- Date: Fri, 25 Sep 2020 16:49:35 -0500 } 7, 54 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 7, 54 -- Gecko/20100101 Thunderbird/68.12.0 } 7, 54 -- MIME-Version: 1.0 } 7, 54 -- In-Reply-To: {202009251532.08PFWd4E028530-at-microscopy.com} } 7, 54 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 7, 54 -- Content-Language: en-US } 7, 54 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
1. you work at a Mag of 30k? most likely, on this TEM, you have a bottom-mount camera installed, haven't you? the last time, when I tested this TEM, it had a bottom-mount camera installed. - IF SO: the pixel size on the detector might be in the range of 0.3 to 0.5 nm. Assuming that you work on an ultrathin section, this pixel size is too small (IMO), and you can easily go to 10k or 15k and save a lot of time for collecting the data. Already at Mag 15k, you have 1/4 of the data and you are about ready in 1/4 or 1/3 of the time (dep. on the overhead during data collection). But you do not loose any relevant information, on sections (I guess).
2. For a full grid montage at this MAG, I can imagine that NO grid is flat enough that you do not have to readjust the focus, from time to time. I see your problem, easily. - I wonder if JEOL can provide a solution for this problem? if they offer something like LLP, then regular focussing is mandatory. Also JEOL should be aware of this - you may contact the application specialists, who are in charge for you.
3. The 1400 Flash can easily be run using SerialEM, with almost most modern cameras (look at the webpage of SerialEM , and ask Guenter Resch - Nexperion, Wien - and David Mastronarde). With SerialEM, it is well possible to perform what you want to do: to have a script collecting the data of a full grid and to do regular Autofocus. - you need SerialEM - then install it or have it installed. And then stage calibration, calibration of image shift and beam shift, and autofocus calibration. Then the wonderful SerialEM Navigator - built in - and a script.
kind regards, Reinhard
-- Prof. Dr. Reinhard Rachel University of Regensburg Centre for EM / Anatomy Faculty of Biology & Preclin. Med. Universitaetsstrasse 31 D-93053 Regensburg - Germany tel +49 941 943 -2837, -1720 mail reinhard.rachel-at-biologie.uni-regensburg.de office: VKL 3.1.29 member of the IFSM board
Next microscopy conferences: - next European EM conf.: EMC2024 in Kopenhagen, August 2024 - MC2021 in Vienna, 22-26 Aug 2021(D-A-CH + MCM conference) - IMC20 in Busan, South Korea: Sept 25-30, 2022 - next Microbiol. conferences: VAAM March 2021 Dsseldorf
==============================Original Headers============================== 12, 25 -- From Reinhard.Rachel-at-biologie.uni-regensburg.de Sun Sep 27 07:24:55 2020 12, 25 -- Received: from mx1.uni-regensburg.de (mx1.uni-regensburg.de [194.94.157.146]) 12, 25 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 08RCOsPc018284 12, 25 -- for {Microscopy-at-microscopy.com} ; Sun, 27 Sep 2020 07:24:55 -0500 12, 25 -- Received: from mx1.uni-regensburg.de (localhost [127.0.0.1]) 12, 25 -- by localhost (Postfix) with SMTP id E6C58600004E 12, 25 -- for {Microscopy-at-microscopy.com} ; Sun, 27 Sep 2020 14:35:03 +0200 (CEST) 12, 25 -- Received: from gwsmtp.uni-regensburg.de (gwsmtp1.uni-regensburg.de [132.199.5.51]) 12, 25 -- by mx1.uni-regensburg.de (Postfix) with ESMTP id C915C600004D 12, 25 -- for {Microscopy-at-microscopy.com} ; Sun, 27 Sep 2020 14:35:03 +0200 (CEST) 12, 25 -- Received: from uni-regensburg-smtp1-MTA by gwsmtp.uni-regensburg.de 12, 25 -- with Novell_GroupWise; Sun, 27 Sep 2020 14:35:03 +0200 12, 25 -- Message-Id: {5F7086F602000054000928F8-at-gwsmtp.uni-regensburg.de} 12, 25 -- X-Mailer: Novell GroupWise Internet Agent 18.2.1 12, 25 -- Date: Sun, 27 Sep 2020 14:35:02 +0200 12, 25 -- From: "Reinhard Rachel" {Reinhard.Rachel-at-biologie.uni-regensburg.de} 12, 25 -- To: {sobol-at-img.cas.cz} 12, 25 -- Cc: "microscopy server" {Microscopy-at-microscopy.com} 12, 25 -- Subject: software script incl automatic focusing 12, 25 -- References: {5F7085EA02000054000928F5-at-gwsmtp.uni-regensburg.de} 12, 25 -- Mime-Version: 1.0 12, 25 -- Content-Type: text/plain; charset=ISO-8859-1 12, 25 -- Content-Disposition: inline 12, 25 -- Content-Transfer-Encoding: 8bit 12, 25 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 08RCOsPc018284 ==============================End of - Headers==============================
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Email: philippe.buffat-at-epfl.ch Name: P.Buffat
Title-Subject: [Filtered] SOS PDF file 00-008-0342
Message: Would somebody be able and so kind to send me a copy of the ICCD (ex-JCPDS) files PDF 00-008-0342 (tetragonal HfO2).
This information is needed to confirm my crystal phase identification made with the ICSD7146 structure, answer to a reviewer who disagree with it and get the final acceptance of a manuscript before Sep 30. Our university ICSD database looks out of order for a week (Corona-virus?). This file is often used in literature for the HfO2 tetragonal phase, but the Bravais parameters, space group and author reference are never explicitly given. Thanks for your help Philippe Buffat
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I am trying to locate a paper which seems to have been published in proceedings of Scanning Electron Microscopy conference held back in 1983 in Chicago, IL by SEM Inc.:
"Measurement of elastically reflected electrons (E { 2.5 keV) for imaging of surfaces in a simple ultra high vacuum scanning electron microscope" R Schmid, KH Gaukler, H Seiler - Scanning Electron Microscopy, 1983
Neither the paper nor proceedings aren't available through library system (at least within my ability to navigate it), so hoping that maybe someone else with persistent habit of reading (and keeping) paper books may have a copy...
Thank you very much beforehand, Valery -- Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 www.partbeamsystech.com www.fibsemproducts.com www.freudlabs.com
I have a question about the EDX line profile measurements for the INCA software users, I hope there are some here :)
We use INCA software and Oxford EDX detector for EDX measurements with our JEOL 2100F TEM. After taking line profile measurements, we have 200 points in the line, for each point one spectrum. How do you extract the data? I only found extraction for each point-spectrum by hand, which is really weird and long for 200 points.
Thank you very much in advance!
With my best wishes, Vita Solovyeva
Dr. Vita Solovyeva University of Oldenburg Fak V- Institute of Physics Carl von Ossietzky-Strae 9-11 26129 Oldenburg Tel. +49-4417983547 Vita.solovyeva-at-uni-oldenburg.de https://uol.de/fk5/elektronenmikroskopie
==============================Original Headers============================== 10, 36 -- From vita.solovyeva-at-uni-oldenburg.de Tue Sep 29 01:22:47 2020 10, 36 -- Received: from smtp130.uni-oldenburg.de (smtp131.uni-oldenburg.de [134.106.141.131]) 10, 36 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 08T6Ml0E002790 10, 36 -- for {Microscopy-at-Microscopy.Com} ; Tue, 29 Sep 2020 01:22:47 -0500 10, 36 -- Received: from smtp130.uni-oldenburg.de (localhost.localdomain [127.0.0.1]) 10, 36 -- by localhost (Email Security Appliance) with SMTP id 6D4E9A08241_F72D51FB 10, 36 -- for {Microscopy-at-Microscopy.Com} ; Tue, 29 Sep 2020 06:33:03 +0000 (GMT) 10, 36 -- Received: from mail.uni-oldenburg.de (ex01.w2kroot.uni-oldenburg.de [10.20.10.80]) 10, 36 -- (using TLSv1.2 with cipher ECDHE-RSA-AES256-SHA384 (256/256 bits)) 10, 36 -- (Client CN "mail.uni-oldenburg.de", Issuer "DFN-Verein Global Issuing CA" (verified OK)) 10, 36 -- by smtp130.uni-oldenburg.de (Sophos Email Appliance) with ESMTPS id 4974EA080F0_F72D51FF 10, 36 -- for {Microscopy-at-Microscopy.Com} ; Tue, 29 Sep 2020 06:33:03 +0000 (GMT) 10, 36 -- Received: from ex04.w2kroot.uni-oldenburg.de (10.20.10.83) by 10, 36 -- ex01.w2kroot.uni-oldenburg.de (10.20.10.80) with Microsoft SMTP Server 10, 36 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_256_CBC_SHA384_P256) id 10, 36 -- 15.1.2044.4; Tue, 29 Sep 2020 08:33:02 +0200 10, 36 -- Received: from ex04.w2kroot.uni-oldenburg.de ([fe80::1434:4bad:c315:5504]) by 10, 36 -- ex04.w2kroot.uni-oldenburg.de ([fe80::1434:4bad:c315:5504%14]) with mapi id 10, 36 -- 15.01.2044.006; Tue, 29 Sep 2020 08:33:02 +0200 10, 36 -- From: "Dr. Vita Solovyeva" {vita.solovyeva-at-uni-oldenburg.de} 10, 36 -- To: "Microscopy-at-Microscopy.Com" {Microscopy-at-Microscopy.Com} 10, 36 -- Subject: TEM: EDX, INCA software, line profile measurements 10, 36 -- Thread-Topic: TEM: EDX, INCA software, line profile measurements 10, 36 -- Thread-Index: AdaWKzukq6u5wLu1T5+9EzGgPlrI/w== 10, 36 -- Date: Tue, 29 Sep 2020 06:33:02 +0000 10, 36 -- Message-ID: {c8ee94deebc14d2f85fd50505debbe0c-at-uni-oldenburg.de} 10, 36 -- Accept-Language: en-US, de-DE 10, 36 -- Content-Language: de-DE 10, 36 -- X-MS-Has-Attach: 10, 36 -- X-MS-TNEF-Correlator: 10, 36 -- x-originating-ip: [134.106.114.187] 10, 36 -- Content-Type: text/plain; charset="iso-8859-1" 10, 36 -- MIME-Version: 1.0 10, 36 -- X-SASI-RCODE: 200 10, 36 -- Content-Transfer-Encoding: 8bit 10, 36 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 08T6Ml0E002790 ==============================End of - Headers==============================
From pyledary522s-at-gmail.com Wed Sep 30 20:36:52 2020 Return-Path: {pyledary522s-at-gmail.com} Received: from gmail.com ([104.148.61.183]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 0911ap7D004677 for {microscopylistserverarchive7-at-microscopy.com} ; Wed, 30 Sep 2020 20:36:52 -0500 Message-ID: {E5068D90.F32246C9-at-gmail.com}
Dear all, Please, could anybody give me some hints on the following problem? We have a coppers grid with ultrathin sections of culture cells. The cells were cultured in the medium containing CuO nanoparticles (NPs). Our colleagues want to prove by EDS that the electron opaque clusters found in the sections have copper. The specimen is unique.
We were aware of the artifact of the additional copper signal induced by the copper grid. Nevertheless, we have tried to record EDS spectra from those clusters and nearby within the ultrathin section. When we measured the spectra in the ultrathin section, we got the artificial Cu-K peak caused by a copper grid and no Cu-L peak. However, we obtained a pretty nice Cu-L peak and Cu-K peak when we measured the spectra from NPs clusters.
EDS spectra acquisition conditions: JEOL F200 (200 kV), 30 ls, all other settings were the standard setting of the microscope - we were unallowed to change any settings.
I am thanking you in advance for any comments.
Best regards, Oldrich
-- Oldřich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group Vídeňská 1083 142 20 Prague 4 Czech Republic
Upozorneni: Neni-li v teto zprave vyslovne uvedeno jinak, ma tato E-mailova zprava nebo jeji prilohy pouze informativni charakter. Tato zprava ani jeji prilohy v zadnem ohledu ustavy AV CR, v.v.i. k nicemu nezavazuji. Text teto zpravy nebo jejich priloh neni navrhem na uzavreni smlouvy, ani prijetim pripadneho navrhu na uzavreni smlouvy, ani jinym pravnim jednanim smerujicim k uzavreni jakekoliv smlouvy a nezaklada predsmluvni odpovednost ustavu AV CR, v.v.i.
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==============================Original Headers============================== 12, 26 -- From benada-at-biomed.cas.cz Wed Oct 7 03:20:13 2020 12, 26 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 12, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0978KDBj016717 12, 26 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2020 03:20:13 -0500 12, 26 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 12, 26 -- (using TLSv1.3 with cipher TLS_AES_256_GCM_SHA384 (256/256 bits) 12, 26 -- key-exchange ECDHE (P-256) server-signature RSA-PSS (2048 bits) server-digest SHA256) 12, 26 -- (No client certificate requested) 12, 26 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 461DFD014BF 12, 26 -- for {microscopy-at-microscopy.com} ; Wed, 7 Oct 2020 10:30:55 +0200 (CEST) 12, 26 -- Date: Wed, 7 Oct 2020 10:30:54 +0200 12, 26 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 12, 26 -- To: {microscopy-at-microscopy.com} 12, 26 -- Subject: EDS - copper grid? 12, 26 -- Message-ID: {20201007103054.3f5c5d89-at-u117ob02} 12, 26 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 12, 26 -- =?UTF-8?B?xIxS?= 12, 26 -- X-Mailer: Claws Mail 3.17.3 (GTK+ 2.24.32; i686-pc-linux-gnu) 12, 26 -- MIME-Version: 1.0 12, 26 -- Content-Type: text/plain; charset=UTF-8 12, 26 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 12, 26 -- X-IoP-CAS-MailScanner-ID: 461DFD014BF.A941B 12, 26 -- X-IoP-CAS-MailScanner: Processed 12, 26 -- X-Spam-Status: No 12, 26 -- Content-Transfer-Encoding: 8bit 12, 26 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 0978KDBj016717 ==============================End of - Headers==============================
I am trying to rebuild the computer for a SEM FEI Quanta 200 on a HP XW 4600.
I have first restored a FEI windows XP image and then installed Quanta_2.42_i1401. I followed all the installation instructions from the pdf which came with this DVD kit. Motion controller didn't work, DSPB pci board does not load the dspb.sys driver correctly.
Then I restored a FEI windows 2k image on the HP XW 4600 and installed Quanta_2.42_i1401. Thsi time motion seams to be working, but I have the same error related to DSPB driver.
Anybody had this issue before? Do I need another dspb.sys? Is there any other file needed for the DSPB board? My board is PM DIG SCAN & PAT BRD DSPB 4022 192 91222
Regards,
-- Cătălin Marinescu, Engr., PhD. +40722233858
Asociația Independent Research Str. Timișului nr 58, sect.1 București - 012416 România www.independent-research.ro
==============================Original Headers============================== 8, 30 -- From Catalin.Marinescu-at-independent-research.ro Wed Oct 7 08:36:08 2020 8, 30 -- Received: from mail.bluescreen.ro (mail.bluescreen.ro [195.238.90.1]) 8, 30 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 097Da70G025100 8, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Oct 2020 08:36:08 -0500 8, 30 -- Received: from localhost (localhost.localdomain [127.0.0.1]) 8, 30 -- by mail.bluescreen.ro (Postfix) with ESMTP id C40E080916E6 8, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Oct 2020 16:46:51 +0300 (EEST) 8, 30 -- DKIM-Filter: OpenDKIM Filter v2.11.0 mail.bluescreen.ro C40E080916E6 8, 30 -- X-Virus-Scanned: amavisd-new at bluescreen.ro 8, 30 -- Received: from mail.bluescreen.ro ([127.0.0.1]) 8, 30 -- by localhost (mail.bluescreen.ro [127.0.0.1]) (amavisd-new, port 10024) 8, 30 -- with LMTP id RMqU3szrmJm7 for {Microscopy-at-microscopy.com} ; 8, 30 -- Wed, 7 Oct 2020 16:46:50 +0300 (EEST) 8, 30 -- Received: from [10.5.5.100] (94-53-35-46.next-gen.ro [94.53.35.46]) 8, 30 -- (using TLSv1.2 with cipher ECDHE-RSA-AES128-GCM-SHA256 (128/128 bits)) 8, 30 -- (No client certificate requested) 8, 30 -- by mail.bluescreen.ro (Postfix) with ESMTPSA id 6B49480916C4 8, 30 -- for {Microscopy-at-Microscopy.Com} ; Wed, 7 Oct 2020 16:46:50 +0300 (EEST) 8, 30 -- DKIM-Filter: OpenDKIM Filter v2.11.0 mail.bluescreen.ro 6B49480916C4 8, 30 -- From: Catalin Marinescu {Catalin.Marinescu-at-independent-research.ro} 8, 30 -- Subject: FEI Quanta 200 DSPB pci board Windows 2000 driver 8, 30 -- To: Microscopy-at-Microscopy.Com 8, 30 -- Message-ID: {08020e6e-6246-e4a4-5e39-275dae9c0749-at-independent-research.ro} 8, 30 -- Date: Wed, 7 Oct 2020 16:46:48 +0300 8, 30 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; WOW64; rv:68.0) Gecko/20100101 8, 30 -- Thunderbird/68.12.1 8, 30 -- MIME-Version: 1.0 8, 30 -- Content-Type: text/plain; charset=utf-8; format=flowed 8, 30 -- Content-Language: en-US 8, 30 -- Content-Transfer-Encoding: 8bit ==============================End of - Headers==============================
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Email: whiteto-at-missouri.edu Name: Tommi White
Title-Subject: [Filtered] Faculty position in Biochemistry at University of Missouri
Message: Dear Microscopy List-serve colleagues, University of Missouri is recruiting for a faculty member (all levels!) in the Department of Biochemistry. Applications are due November 16th, 2020. https://biochem.missouri.edu/open-positions/ CryoEM is an area we wish to strengthen, including in situ cryoET. The Universitys Electron Microscopy Core will be installing the following new microscopes in the $260M NextGen Precision Health Institute: Titan Krios G4 CryoTEM with Bioquantum K3, Aquilos 2, Leica Thunder CryoCLEM and VolumeScope. Additional instruments to be added include Helios Hydra UX PFIB with SIMS-TOF and Spectra Aberration-Corrected TEM with Continuum K3. These additions are part of a recent partnership with Thermo Fisher Scientific to create a Center of Excellence in Electron Microscopy with the University of Missouri System focused on 3 areas: instrumentation innovation, research/collaboration between basic/clinical sciences, and workforce development with STEM training and education. This is a great opportunity to get on the ground floor and help us build something amazing. Please feel free to reach out to me with any questions! We look forward to your applications and would appreciate if you could let interested colleagues know too! Tommi A. White, Ph.D. Director, Electron Microscopy Core Assistant Research Professor, Biochemistry University of Missouri Mail: W117 Veterinary Medicine Bldg 1520 E Rollins St., Columbia, MO 65211 EMC: 573-882-8304 Direct: 573-884-7338 Email: whiteto-at-missouri.edu Web: http://emc.missouri.edu Tweets:-at-MizzouEMC
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From tammyhoward072fu-at-gmail.com Mon Oct 12 14:26:32 2020 Return-Path: {tammyhoward072fu-at-gmail.com} Received: from gmail.com ([104.148.61.169]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 09CJQWjn002409 for {microscopylistserverarchive7-at-microscopy.com} ; Mon, 12 Oct 2020 14:26:32 -0500 Received: from unknown (HELO mtu23.bigping.com) (Tue, 13 Oct 2020 00:31:39 +0500) by smtp.doneohx.com with SMTP; Tue, 13 Oct 2020 00:31:39 +0500 Received: from mmx09.tilkbans.com ([Tue, 13 Oct 2020 00:20:38 +0500]) by snmp.otwaloow.com with ASMTP; Tue, 13 Oct 2020 00:20:38 +0500 Received: from [120.11.171.169] by nntp.pinxodet.net with SMTP; Tue, 13 Oct 2020 00:06:52 +0500 Message-ID: {2A363FD7.4632942F-at-gmail.com}
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Were hiring! CMCA-at-UWA are currently advertising two Research Fellow (Level B) positions, which are available for immediate start. If you have the right skills and like sun, surf, short commutes, and microscopes apply now!
1. *CryoTEM specialist*
Exciting opportunity for a passionate researcher with cryoTEM experience to be a key player in the establishment of the first cryoTEM facility in Western Australia and to work in a multi-disciplinary research environment developing and supporting imaging and diffraction applications. Fixed term appointment until 30 June 2023 with an immediate start. Salary range: Level B $100,374 p.a. - $118,776 p.a. plus superannuation
Applications are open to researchers with skills in microscopy techniques and/or associated data processing methods who are looking for an exciting opportunity to develop novel multi-modal applications in a dynamic, interdisciplinary team environment. Fixed term appointment until 30 June 2023 with an immediate start. Salary range: Level B $100,374 p.a. - $118,776 p.a. plus superannuation
Hello everyone, I've been asked to help out by distinguishing between type E, R and D glass by elemental composition from EDS analysis. I thought I have references to the elemental composition from past standards, but they apparently have been place somewhere so safe I can't find them. I found several references but the most useful combined Ca and Mg as a single measurement.
Unfortunately we don't have standards, otherwise I'd simple re-run them.
Any help would be useful.
Thanks!
Stay calm...Be brave....watch for signs
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
X-from: Smart, Thomas Philip {tsmart-at-eastman.com}
Hello Frank, With a lack of standards to reference, the Saint Gobain website https://glassproperties.com/glasses/E_R_and_D_glass_properties.pdf shows you should be able to tell the difference between the three types of glass by general elemental composition... notably the Al, Ca, and B levels in each. Hope this helps you.
Thomas
Thomas Smart Microscopy Scientist Eastman Chemical Company
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I thought I have references to the elemental composition from past } standards, but they apparently have been place somewhere so safe I can't find them. } I found several references but the most useful combined Ca and Mg as a single measurement. } } Unfortunately we don't have standards, otherwise I'd simple re-run them. } } Any help would be useful. } } Thanks! } } } } } Stay calm...Be brave....watch for signs } } Frank Karl } Microscopist } Akron Rubber Development Laboratory } 2887 Gilchrist Road } Akron, Ohio 44305 } } } ==============================Original Headers============================== } 11, 53 -- From microscopy.listserver-at-gmail.com Tue Oct 13 15:39:52 2020 } 11, 53 -- Received: from mail-il1-f175.google.com (mail-il1-f175.google.com [209.85.166.175]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 09DKdq6u025653 } 11, 53 -- for {microscopy-at-microscopy.com} ; Tue, 13 Oct 2020 15:39:52 -0500 } 11, 53 -- Received: by mail-il1-f175.google.com with SMTP id l16so2167637ilj.9 } 11, 53 -- for {microscopy-at-microscopy.com} ; Tue, 13 Oct 2020 13:50:57 -0700 (PDT) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=PXZbD32bZq394s7j/VQ8pWYzSAdjzuyu9gy/L/iBi+o=; } 11, 53 -- b=jYIlRrhSQfirorlv0in12+xSSBi0ObpagA51otl76xINXPuxDppb0QglddzIZyntdO } 11, 53 -- BNlWpv/PiUdAtSN2laiiSAaMFL8/vwmf9kFff5NmFf0cL23m7ZtvLJ3cMSjMe4M2B+lQ } 11, 53 -- MGdkSwFuE3cBTfCZrcR/nD8iiMRICfdSxxjuSDAmqcUR8BFaYNmZ8FfYCVsxKR550158 } 11, 53 -- OphVFjiuDv0FRxcRwYv6xnwVgiST1U2uDYdFL6L7Mu/v66zu1kI7x/UzFkRcqI1PdtqV } 11, 53 -- 6i7bLdLw86zFhQsutexlZLGDe92vsmR89/S7YHExXWrtdDCdnB/btIrXfrEyvHpkuiES } 11, 53 -- H1IA== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=PXZbD32bZq394s7j/VQ8pWYzSAdjzuyu9gy/L/iBi+o=; } 11, 53 -- b=AEcXNRzWtPNHGAOhCNARC9BCnkbu5UZWoOD+71Bmm74dycRN9O06Ii0W/UxHssZmnG } 11, 53 -- omX7uYnyftnLlUHqBhQRGvvnGGNM04Mpl15gbgOcQg/pIbdqWbOAzvCYsrbv4Mb5BvzV } 11, 53 -- JOAckX68/kJ3T0F+TyS7VKiEwoVAicJ230XXd0LHxWTZAp/Mkh5kcjPdDXaZs9X+r/iM } 11, 53 -- KDaU7P18m2PiDKsoqwi58xZalFN+cHzSJPYKd1vGhJcFGOWenTTQXqICC0R4PYd0peo3 } 11, 53 -- +UfShr1mkhLAOjiMTcKcurPDc2hqjhP0HeUHQPYhJ53nMIiH5mODuGfqFJW5COxwZSBg } 11, 53 -- J2CQ== } 11, 53 -- X-Gm-Message-State: AOAM530/JQ3wRCIoFHFFEOtDcad3yQuw8mkIsZreElenqxFhaXyft76W } 11, 53 -- oyLA+bwHaMxnkSJgAfc8VgbE6M6FPvE2srN4 } 11, 53 -- X-Google-Smtp-Source: } ABdhPJzviP79H2MQR7/czqLmms8/9euDoDGMKeKfZiRnce1r4GYi+81zi5gZ7kmQuhCFgWWaJ70ijQ== } 11, 53 -- X-Received: by 2002:a92:7f05:: with SMTP id a5mr1561133ild.112.1602622257019; } 11, 53 -- Tue, 13 Oct 2020 13:50:57 -0700 (PDT) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net } ([2603:300a:f04:7100:25cd:6b45:27f9:b026]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id y6sm849805ili.36.2020.10.13.13.50.56 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 11, 53 -- Tue, 13 Oct 2020 13:50:56 -0700 (PDT) } 11, 53 -- Subject: EDS on Glass } 11, 53 -- References: {BN7PR15MB2260CE0FA91F619B266FEA4A95070-at-BN7PR15MB2260.namprd15.prod.outlook.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: } {BN7PR15MB2260CE0FA91F619B266FEA4A95070-at-BN7PR15MB2260.namprd15.prod.outlook.com} } 11, 53 -- Message-ID: {6f537b67-97d7-beb0-a410-4f6b1872dd65-at-gmail.com} } 11, 53 -- Date: Tue, 13 Oct 2020 15:50:55 -0500 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 11, 53 -- Gecko/20100101 Thunderbird/68.12.1 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: } {BN7PR15MB2260CE0FA91F619B266FEA4A95070-at-BN7PR15MB2260.namprd15.prod.outlook.com} } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
On Tue, Oct 13, 2020 at 4:51 PM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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X-from: Frank Karl {frank_karl-at-ardl.com {mailto:frank_karl-at-ardl.com} }
Hello everyone, I've been asked to help out by distinguishing between type E, R and D glass by elemental composition from EDS analysis. I thought I have references to the elemental composition from past standards, but they apparently have been place somewhere so safe I can't find them. I found several references but the most useful combined Ca and Mg as a single measurement.
Unfortunately we don't have standards, otherwise I'd simple re-run them.
Any help would be useful.
Thanks!
Stay calm...Be brave....watch for signs
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
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Title-Subject: [Filtered] Job opening for a PhD position in cryo-EM and correlative microscopy
Message: The MPI for Polymer Research is currently advertising a PhD position in correlative microscopy of colloids. This position offers the great opportunity to work with a unique cryo-TEM. Currently we are installing a Titan Krios G4 with a K3 DED Continuum EELS. The research project includes the development of novel strategies to correlate the information from superresolution light microscopy with the high resolution information from the EM. Detailed information can be found here: www.mpip-mainz.mpg.de/500074/PhD_position_in_correlative_microscopy_of_colloids_2?c=102396
or just contact me by e-mail (lieberw-at-mpip-mainz.mpg.de).
Best, Ingo
__________________________________________________ Dr. Ingo Lieberwirth Head of EM core facility MPI for Polymer Research Mainz, Germany
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X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz}
Hi
We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB). The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we load a FIB produced slice mounted on a carbon film coated copper grid, the slice disappears within a short time of the beam going onto it. The hole remaining in the carbon film is the same shape as the original slice so I suspect that the slice is heating up and burning a hole in the film. The remainder of the film remains intact.
-----Original Message----- X-from: microscopy.listserver-at-gmail.com To: ls3foxdelta-at-verizon.net Sent: Tue, Oct 13, 2020 4:47 pm
X-from: Frank Karl {frank_karl-at-ardl.com {mailto:frank_karl-at-ardl.com} }
Hello everyone, I've been asked to help out by distinguishing between type E, R and D glass by elemental composition from EDS analysis. I thought I have references to the elemental composition from past standards, but they apparently have been place somewhere so safe I can't find them. I found several references but the most useful combined Ca and Mg as a single measurement.
Unfortunately we don't have standards, otherwise I'd simple re-run them.
Any help would be useful.
Thanks!
Stay calm...Be brave....watch for signs
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
It could be sample heating, sample charging, the carbon sputtering away, or some mechanical vibration of the sample is the culprit of your film failures. Carbon films are resilient at lower beam currents but can quickly be sputtered away during STEM experiments and when higher beam currents are used in TEM mode, so it's possible the aggressive beam current settings needed for good EBSD are sputtering the carbon away (assuming lamella is electron transparent; if thick, then the excitation volume will be mostly confined to your sample). If your sample is an insulator, it may be charging and physically vibrating during the discharge-charge cycle and punching a hole in the carbon film. Depending on the thickness of the lamella and how conductive it is, you could be heating the lamella to higher temperatures during the EBSD acquisition. Carbon films are generally fine up to hundreds of celsius, but the local heating of the lamella may be stressing the carbon film and causing it to rupture. You could also be puncturing the film slightly during the landing of the lamella and the electron beam will easily shred the carbon film. Lots of things to consider, and quite complicated depending on how thick the lamella is.
Are you using "thick" carbon films or ultrathin? Are you able to anchor the FIB lamella to one of the metal grid bars using Pt/W deposition? Could you land the lamella as close to a corner of the carbon foil? Alternatively, if you're able to change support films SiN windows are very mechanically robust and unlikely to fail. If you need to use carbon films, you may try to use a lacey or holey carbon film and anchor the lamella using Pt/W to a couple of carbon filaments. Not trivial to accomplish but it's been done before. I suspect you're trying to do something in situ with the lamella, so your options may be limited.
Good luck, Chris
On Thu, Oct 15, 2020 at 8:02 AM {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz} } } Hi } } We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB). } The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we } load a FIB produced slice mounted on a carbon film coated copper grid, the slice disappears within } a short time of the beam going onto it. } The hole remaining in the carbon film is the same shape as the original slice so I suspect that the } slice is heating up and burning a hole in the film. The remainder of the film remains intact. } } Any thoughts on how to mitigate this? } } Thanks } } Allan } } } ==============================Original Headers============================== } 7, 53 -- From microscopy.listserver-at-gmail.com Thu Oct 15 06:42:05 2020 } 7, 53 -- Received: from mail-il1-f177.google.com (mail-il1-f177.google.com [209.85.166.177]) } 7, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 09FBg5Ud009299 } 7, 53 -- for {microscopy-at-microscopy.com} ; Thu, 15 Oct 2020 06:42:05 -0500 } 7, 53 -- Received: by mail-il1-f177.google.com with SMTP id p16so3706194ilq.5 } 7, 53 -- for {microscopy-at-microscopy.com} ; Thu, 15 Oct 2020 04:53:16 -0700 (PDT) } 7, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 53 -- d=gmail.com; s=20161025; } 7, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 7, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 7, 53 -- bh=3Yei7pjUTFYggTdLpgupo2ZTJWUjRAG7l/W3FqyCK5s=; } 7, 53 -- b=EG9hvIuj5ExXVHj5a5BGxTC/ISPAhu0ZXbniJN6+kIRScPeGpNXiWIww+tvYBJKGy6 } 7, 53 -- nZMFxEQg3TCvC6ZxO2s0ThIuFKCSgHVw9W73zgvinYNDzhJSPmrTDE3+HTVrciO6ojy4 } 7, 53 -- TFp22WMoqk90An8lXc4axWn75E0g2n57PwDmJRsyFShj+DNGoYr5z/fNZ4slix/SVJv1 } 7, 53 -- 6TyBVlA7qD5mfbh84fw+sUILlheWlO8/KCSB9lOyYutsJgg5aJHPKtqGywfy+kZswphb } 7, 53 -- pIrS3bc+lleecOBghfvjckiaqg2AxBTQMJwED49awHWpTIX7F27rW5e3/V0Jel1H6Nhp } 7, 53 -- XOlw== } 7, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 53 -- d=1e100.net; s=20161025; } 7, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 7, 53 -- :user-agent:mime-version:in-reply-to:content-language } 7, 53 -- :content-transfer-encoding; } 7, 53 -- bh=3Yei7pjUTFYggTdLpgupo2ZTJWUjRAG7l/W3FqyCK5s=; } 7, 53 -- b=Nd2aFAV/DHVmrfHRwJA7pGRdzcOMAhVAkGfXqL118SHg9zujV+wDzxc/ttre4OBAj2 } 7, 53 -- bV+sUctb12FtpsWvrb+LIO0PsGCxSW95u1NMcdFbIz8I3a6lxQ42QBIv5u7t8kyfFL5m } 7, 53 -- U837GVIEZcGHv8MbKuQwrvwjzYAyfq/fxk2lcWonGEhIfxcEv9C93csmm/gXyhxAP6zT } 7, 53 -- GBaeM9MiZIhdKYWvx27zcKHWaxc5LanxsZq97mOuspbad67DXZojMPFCULcEW7zk1xec } 7, 53 -- s66OLD/WSlvuPqexsOMAIExTPWxA/+8MsPH8kbMEZrGxaS4KmXHTQ5FyoMtQxxmED1hu } 7, 53 -- S8ng== } 7, 53 -- X-Gm-Message-State: AOAM531nIpbRI6+xp1OUtNam1Tn3tygEzz7S/g/MxNlgK4xCuxNfuDm6 } 7, 53 -- OdSsDNd+0bdKbrBgo7bcLRkFeSR6pomU+dTO } 7, 53 -- X-Google-Smtp-Source: ABdhPJxmtqM9Uyce5h46ANggDjMSJJ0mK/5Qxh/CVQQChAXdgGX9qA49M6AY8uUFX9HXRAxvV5thWg== } 7, 53 -- X-Received: by 2002:a92:ba8d:: with SMTP id t13mr2868019ill.131.1602762795653; } 7, 53 -- Thu, 15 Oct 2020 04:53:15 -0700 (PDT) } 7, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:d987:54be:4634:38b9]) } 7, 53 -- by smtp.googlemail.com with ESMTPSA id x14sm2310710ilg.21.2020.10.15.04.53.15 } 7, 53 -- for {microscopy-at-microscopy.com} } 7, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 7, 53 -- Thu, 15 Oct 2020 04:53:15 -0700 (PDT) } 7, 53 -- Subject: Fwd: Thin dense slice burning hole in carbon film during EBSD } 7, 53 -- References: {1462455C-C636-4AC5-A431-459C4A8C1565-at-otago.ac.nz} } 7, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 7, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 7, 53 -- X-Forwarded-Message-Id: {1462455C-C636-4AC5-A431-459C4A8C1565-at-otago.ac.nz} } 7, 53 -- Message-ID: {eee7e172-5986-16c9-4d02-5af615de4b4e-at-gmail.com} } 7, 53 -- Date: Thu, 15 Oct 2020 06:53:14 -0500 } 7, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 7, 53 -- Gecko/20100101 Thunderbird/68.12.1 } 7, 53 -- MIME-Version: 1.0 } 7, 53 -- In-Reply-To: {1462455C-C636-4AC5-A431-459C4A8C1565-at-otago.ac.nz} } 7, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 7, 53 -- Content-Language: en-US } 7, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
==============================Original Headers============================== 9, 55 -- From crwinkler-at-ncsu.edu Thu Oct 15 10:13:34 2020 9, 55 -- Received: from mail-il1-f177.google.com (mail-il1-f177.google.com [209.85.166.177]) 9, 55 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 09FFDYvB023243 9, 55 -- for {Microscopy-at-microscopy.com} ; Thu, 15 Oct 2020 10:13:34 -0500 9, 55 -- Received: by mail-il1-f177.google.com with SMTP id p16so4634063ilq.5 9, 55 -- for {Microscopy-at-microscopy.com} ; Thu, 15 Oct 2020 08:24:45 -0700 (PDT) 9, 55 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 55 -- d=ncsu.edu; s=google; 9, 55 -- h=mime-version:references:in-reply-to:from:date:message-id:subject:to 9, 55 -- :cc; 9, 55 -- bh=+wy6Tyz89vxn8skQqfQtibMfNHqiSnZp00Ldgkjb7Hk=; 9, 55 -- b=gJ7JZIz8oOOTpJ34M0W8bvGXdpilcc6V3diu2bcL1EVIi2I/iUS8JJHtCQydFOKiXV 9, 55 -- 7nVp+pHXD3FMwpRdtCq7S/TwkND1QhF5jC6xscQsjitPertmLR82obs2dP3j7mIEc99K 9, 55 -- xyMa6Gis8iUI0eulOpk6T4/6auByyMiRcfcINYYX39ISOcHfBeythhWDetfRvgt5fuyT 9, 55 -- AhLx20voWiRcbv8qGv7IVyEoBLHfLf/0D2PZaui+OaAvWX7B1C3eF8Lbos2MkGJPOh7l 9, 55 -- X9a32Lat0p+sQtrlsc23ryIO5bDSXM+4T7vXhbdxZUwcepNYqpY8FRliLwhLHssE6M9q 9, 55 -- h+iw== 9, 55 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 9, 55 -- d=1e100.net; s=20161025; 9, 55 -- h=x-gm-message-state:mime-version:references:in-reply-to:from:date 9, 55 -- :message-id:subject:to:cc; 9, 55 -- bh=+wy6Tyz89vxn8skQqfQtibMfNHqiSnZp00Ldgkjb7Hk=; 9, 55 -- b=e7G/7MbQkaml/ujXovI4LS0Z5CfgKJ58EOHGs8sYE/eLwNxiiXfhz/6y+oOmUBeKEv 9, 55 -- A6pFCTwJLycV6RlI03Vvgy598WwTrI7Em3WtbhcQhF6/kNCXW6TzQiXeHbmZc2RdQ9br 9, 55 -- UDnAycaG+SPpvEGhjvRw+dIslBpyAjGrc2N2Frp+7agYThlfYUcjuxgrq8N4ll1qpe62 9, 55 -- xy+3QPjmj2g+b5Bv7V/MbVTsaawuH/X6fISJWtn3hXv1leZwvkAawLxBRuAK3Z/1+uy7 9, 55 -- vU2+qbm80OwlFlGU3UMbHgTqVRAoaHkEL1ONm+AmtTgrcV0QK3prikiAU2+tPPIu98F/ 9, 55 -- V0Ew== 9, 55 -- X-Gm-Message-State: AOAM531IjFvLmseFnRmtJnOFqyYvqJ7Vka9Aj0uPfuSEaDe0z4JUwYiD 9, 55 -- vRli+m9qtLNB6DKkqIhTXokrIPUyXkHjZBCB9/srKHBS3QhSXduydaZacXDtH4cMJ27mhnENxm8 9, 55 -- Pzm1iR7xeBWZivlY5tYrBVc9I/o/brYX9QKIHrAHc98wA5nQvnmcV+VxSXU2/FzO9mh/X3kM= 9, 55 -- X-Google-Smtp-Source: ABdhPJyuP7EcymWd0p3gGnDMnrwwlNx7uN57gLmzvqv8Vp0KML8OTz7LZl8qLO9/auDvWKUM9OePIw== 9, 55 -- X-Received: by 2002:a92:d28b:: with SMTP id p11mr3652113ilp.264.1602775484321; 9, 55 -- Thu, 15 Oct 2020 08:24:44 -0700 (PDT) 9, 55 -- Received: from mail-il1-f172.google.com (mail-il1-f172.google.com. [209.85.166.172]) 9, 55 -- by smtp.gmail.com with ESMTPSA id a11sm2622678ilk.81.2020.10.15.08.24.43 9, 55 -- for {Microscopy-at-microscopy.com} 9, 55 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 9, 55 -- Thu, 15 Oct 2020 08:24:43 -0700 (PDT) 9, 55 -- Received: by mail-il1-f172.google.com with SMTP id p16so4633951ilq.5 9, 55 -- for {Microscopy-at-microscopy.com} ; Thu, 15 Oct 2020 08:24:43 -0700 (PDT) 9, 55 -- X-Received: by 2002:a05:6e02:df0:: with SMTP id m16mr3462859ilj.220.1602775483101; 9, 55 -- Thu, 15 Oct 2020 08:24:43 -0700 (PDT) 9, 55 -- MIME-Version: 1.0 9, 55 -- References: {202010151151.09FBpSqn028781-at-microscopy.com} 9, 55 -- In-Reply-To: {202010151151.09FBpSqn028781-at-microscopy.com} 9, 55 -- From: Christopher Winkler {crwinkler-at-ncsu.edu} 9, 55 -- Date: Thu, 15 Oct 2020 11:24:32 -0400 9, 55 -- X-Gmail-Original-Message-ID: {CAA8T2PPXA70ETdFzM=ri410QpS34nZoXQ7fwMuNSqupNGwqcFg-at-mail.gmail.com} 9, 55 -- Message-ID: {CAA8T2PPXA70ETdFzM=ri410QpS34nZoXQ7fwMuNSqupNGwqcFg-at-mail.gmail.com} 9, 55 -- Subject: Re: [Microscopy] Fwd: Thin dense slice burning hole in carbon film 9, 55 -- during EBSD 9, 55 -- To: Microscopy-at-microscopy.com 9, 55 -- Cc: Christopher Winkler {crwinkler-at-ncsu.edu} , allan.mitchell-at-otago.ac.nz 9, 55 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
Some details on the nature of the sample could have been helpful, but if I had to guess:
Try doing FIB deposition on SiN window instead of C grid. Prior to depositing sample, etc..., coat SiN window with C evaporation and make sure to provide reliable connection to ground for charge dissipation.
If the "dense slice" isn't conductive it may be blowing up by ESD due to charging by high electron beam current - if so you could try depositing couple of nm C on the side facing away from EBSD camera, and lowering acceleration voltage.
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 www.partbeamsystech.com www.fibsemproducts.com www.freudlabs.com
"Only the Paranoid Survive" (A.Grove & SpaceX QA)
On 10/15/2020 7:42 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz} } } Hi } } We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB). } The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we } load a FIB produced slice mounted on a carbon film coated copper grid, the slice disappears within } a short time of the beam going onto it. } The hole remaining in the carbon film is the same shape as the original slice so I suspect that the } slice is heating up and burning a hole in the film. The remainder of the film remains intact. } } Any thoughts on how to mitigate this? } } Thanks } } Allan } } } ==============================Original Headers============================== } 7, 53 -- From microscopy.listserver-at-gmail.com Thu Oct 15 06:42:05 2020 } 7, 53 -- Received: from mail-il1-f177.google.com (mail-il1-f177.google.com [209.85.166.177]) } 7, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 09FBg5Ud009299 } 7, 53 -- for {microscopy-at-microscopy.com} ; Thu, 15 Oct 2020 06:42:05 -0500 } 7, 53 -- Received: by mail-il1-f177.google.com with SMTP id p16so3706194ilq.5 } 7, 53 -- for {microscopy-at-microscopy.com} ; Thu, 15 Oct 2020 04:53:16 -0700 (PDT) } 7, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 53 -- d=gmail.com; s=20161025; } 7, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 7, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 7, 53 -- bh=3Yei7pjUTFYggTdLpgupo2ZTJWUjRAG7l/W3FqyCK5s=; } 7, 53 -- b=EG9hvIuj5ExXVHj5a5BGxTC/ISPAhu0ZXbniJN6+kIRScPeGpNXiWIww+tvYBJKGy6 } 7, 53 -- nZMFxEQg3TCvC6ZxO2s0ThIuFKCSgHVw9W73zgvinYNDzhJSPmrTDE3+HTVrciO6ojy4 } 7, 53 -- TFp22WMoqk90An8lXc4axWn75E0g2n57PwDmJRsyFShj+DNGoYr5z/fNZ4slix/SVJv1 } 7, 53 -- 6TyBVlA7qD5mfbh84fw+sUILlheWlO8/KCSB9lOyYutsJgg5aJHPKtqGywfy+kZswphb } 7, 53 -- pIrS3bc+lleecOBghfvjckiaqg2AxBTQMJwED49awHWpTIX7F27rW5e3/V0Jel1H6Nhp } 7, 53 -- XOlw== } 7, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 53 -- d=1e100.net; s=20161025; } 7, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 7, 53 -- :user-agent:mime-version:in-reply-to:content-language } 7, 53 -- :content-transfer-encoding; } 7, 53 -- bh=3Yei7pjUTFYggTdLpgupo2ZTJWUjRAG7l/W3FqyCK5s=; } 7, 53 -- b=Nd2aFAV/DHVmrfHRwJA7pGRdzcOMAhVAkGfXqL118SHg9zujV+wDzxc/ttre4OBAj2 } 7, 53 -- bV+sUctb12FtpsWvrb+LIO0PsGCxSW95u1NMcdFbIz8I3a6lxQ42QBIv5u7t8kyfFL5m } 7, 53 -- U837GVIEZcGHv8MbKuQwrvwjzYAyfq/fxk2lcWonGEhIfxcEv9C93csmm/gXyhxAP6zT } 7, 53 -- GBaeM9MiZIhdKYWvx27zcKHWaxc5LanxsZq97mOuspbad67DXZojMPFCULcEW7zk1xec } 7, 53 -- s66OLD/WSlvuPqexsOMAIExTPWxA/+8MsPH8kbMEZrGxaS4KmXHTQ5FyoMtQxxmED1hu } 7, 53 -- S8ng== } 7, 53 -- X-Gm-Message-State: AOAM531nIpbRI6+xp1OUtNam1Tn3tygEzz7S/g/MxNlgK4xCuxNfuDm6 } 7, 53 -- OdSsDNd+0bdKbrBgo7bcLRkFeSR6pomU+dTO } 7, 53 -- X-Google-Smtp-Source: ABdhPJxmtqM9Uyce5h46ANggDjMSJJ0mK/5Qxh/CVQQChAXdgGX9qA49M6AY8uUFX9HXRAxvV5thWg== } 7, 53 -- X-Received: by 2002:a92:ba8d:: with SMTP id t13mr2868019ill.131.1602762795653; } 7, 53 -- Thu, 15 Oct 2020 04:53:15 -0700 (PDT) } 7, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:d987:54be:4634:38b9]) } 7, 53 -- by smtp.googlemail.com with ESMTPSA id x14sm2310710ilg.21.2020.10.15.04.53.15 } 7, 53 -- for {microscopy-at-microscopy.com} } 7, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 7, 53 -- Thu, 15 Oct 2020 04:53:15 -0700 (PDT) } 7, 53 -- Subject: Fwd: Thin dense slice burning hole in carbon film during EBSD } 7, 53 -- References: {1462455C-C636-4AC5-A431-459C4A8C1565-at-otago.ac.nz} } 7, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 7, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 7, 53 -- X-Forwarded-Message-Id: {1462455C-C636-4AC5-A431-459C4A8C1565-at-otago.ac.nz} } 7, 53 -- Message-ID: {eee7e172-5986-16c9-4d02-5af615de4b4e-at-gmail.com} } 7, 53 -- Date: Thu, 15 Oct 2020 06:53:14 -0500 } 7, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 7, 53 -- Gecko/20100101 Thunderbird/68.12.1 } 7, 53 -- MIME-Version: 1.0 } 7, 53 -- In-Reply-To: {1462455C-C636-4AC5-A431-459C4A8C1565-at-otago.ac.nz} } 7, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 7, 53 -- Content-Language: en-US } 7, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
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Email: nanotech-at-psd1.org Name: John Weisenfeld
Title-Subject: [Filtered] Looking For: Industry Certifications Suitable for High School Students
Message: Hello all!
I'm a High School CTE (Career and Technical Education) teacher. I'm looking for some pointers to Certifications / Certificates / Qualifications that a High School Student could earn to put on their re'sume' if they are interested in industry jobs.
We have a Hitachi TableTop SEM at our school so we have access to some equipment that students can demonstrate proficiency.
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I'm looking for information on the magnetic field produced by the objective lens of a typical 200 kVTEM with a focal length of roughly 3 mm, for examplethe Jeol 2100 with the cryo pole piece.
Also I'd like to know what the field would be if the lens is weakened so much that the focal length is in the order of 2 to 3 cm.
Does anybody have plots or values for (some of) this, or is there some free software to do the calculations with realistic pole piece geometries?
All the best,
Philip
==============================Original Headers============================== 7, 46 -- From koeck-at-kth.se Fri Oct 16 02:15:37 2020 7, 46 -- Received: from smtp-3.sys.kth.se (smtp-3.sys.kth.se [130.237.48.192]) 7, 46 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 09G7Falj028890 7, 46 -- for {microscopy-at-microscopy.com} ; Fri, 16 Oct 2020 02:15:37 -0500 7, 46 -- Received: from smtp-3.sys.kth.se (localhost.localdomain [127.0.0.1]) 7, 46 -- by smtp-3.sys.kth.se (Postfix) with ESMTP id AF62BA85D 7, 46 -- for {microscopy-at-microscopy.com} ; Fri, 16 Oct 2020 09:26:49 +0200 (CEST) 7, 46 -- X-Virus-Scanned: by amavisd-new at kth.se 7, 46 -- Received: from smtp-3.sys.kth.se ([127.0.0.1]) 7, 46 -- by smtp-3.sys.kth.se (smtp-3.sys.kth.se [127.0.0.1]) (amavisd-new, port 10024) 7, 46 -- with LMTP id F9KVRBeqmV3z for {microscopy-at-microscopy.com} ; 7, 46 -- Fri, 16 Oct 2020 09:26:49 +0200 (CEST) 7, 46 -- Received: from exdb01.ug.kth.se (exdb01.ug.kth.se [192.168.32.111]) 7, 46 -- by smtp-3.sys.kth.se (Postfix) with ESMTPS id 103CFA802 7, 46 -- for {microscopy-at-microscopy.com} ; Fri, 16 Oct 2020 09:26:49 +0200 (CEST) 7, 46 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=kth.se; s=default; 7, 46 -- t=1602833209; bh=qxrv8WxbvK6HojvE2YiCmBMjokXQ1FY9q3W355xDj28=; 7, 46 -- h=From:To:Subject:Date:References:In-Reply-To; 7, 46 -- b=BlRRgvITOJNKGRF/wjcBRONaacJYFdkCoG5e7ZYH/FsUdR/Mo3JrHDxtYaR1Fz5LI 7, 46 -- YhvqrqiafRNxZiaTkp8yXjO43tYBdoI+NEkEFZxi3yL6HUaIf6dnvc28WxmzP3Icly 7, 46 -- 9ryxIm8K6owSMSgXcFtRJ5baFodCIOsMc05viggM= 7, 46 -- Received: from exdb01.ug.kth.se (192.168.32.111) by exdb01.ug.kth.se 7, 46 -- (192.168.32.111) with Microsoft SMTP Server (TLS) id 15.0.1497.2; Fri, 16 Oct 7, 46 -- 2020 09:26:48 +0200 7, 46 -- Received: from exdb01.ug.kth.se ([192.168.32.111]) by exdb01.ug.kth.se 7, 46 -- ([192.168.32.111]) with mapi id 15.00.1497.006; Fri, 16 Oct 2020 09:26:48 7, 46 -- +0200 7, 46 -- From: =?iso-8859-1?Q?Philip_K=F6ck?= {koeck-at-kth.se} 7, 46 -- To: "microscopy-at-microscopy.com" {microscopy-at-microscopy.com} 7, 46 -- Subject: VB: Magnetic field in TEM 7, 46 -- Thread-Topic: Magnetic field in TEM 7, 46 -- Thread-Index: AQHWo4vz1V6o90ZjFkaAWkhnuAfKyamZ09NI 7, 46 -- Date: Fri, 16 Oct 2020 07:26:47 +0000 7, 46 -- Message-ID: {1602833207675.98924-at-kth.se} 7, 46 -- References: {1602833017345.77199-at-kth.se} 7, 46 -- In-Reply-To: {1602833017345.77199-at-kth.se} 7, 46 -- Accept-Language: sv-SE, en-US 7, 46 -- Content-Language: sv-SE 7, 46 -- X-MS-Has-Attach: 7, 46 -- X-MS-TNEF-Correlator: 7, 46 -- x-ms-exchange-transport-fromentityheader: Hosted 7, 46 -- x-originating-ip: [46.59.23.142] 7, 46 -- Content-Type: text/plain; charset="iso-8859-1" 7, 46 -- MIME-Version: 1.0 7, 46 -- Content-Transfer-Encoding: 8bit 7, 46 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 09G7Falj028890 ==============================End of - Headers==============================
Some details on the nature of the sample could have been helpful, but if I had to guess:
Try doing FIB deposition on SiN window instead of C grid. Prior to depositing sample, etc..., coat SiN window with C evaporation and make sure to provide reliable connection to ground for charge dissipation.
If the "dense slice" isn't conductive it may be blowing up by ESD due to charging by high electron beam current - if so you could try depositing couple of nm C on the side facing away from EBSD camera, and lowering acceleration voltage.
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 www.partbeamsystech.com www.fibsemproducts.com www.freudlabs.com
"Only the Paranoid Survive" (A.Grove & SpaceX QA)
On 10/15/2020 7:42 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz} } } Hi } } We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB). } The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we } load a FIB produced slice mounted on a carbon film coated copper grid, the slice disappears within } a short time of the beam going onto it. } The hole remaining in the carbon film is the same shape as the original slice so I suspect that the } slice is heating up and burning a hole in the film. The remainder of the film remains intact. } } Any thoughts on how to mitigate this? } } Thanks } } Allan } } } ==============================Original Headers============================== } 7, 53 -- From microscopy.listserver-at-gmail.com Thu Oct 15 06:42:05 2020 } 7, 53 -- Received: from mail-il1-f177.google.com (mail-il1-f177.google.com [209.85.166.177]) } 7, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 09FBg5Ud009299 } 7, 53 -- for {microscopy-at-microscopy.com} ; Thu, 15 Oct 2020 06:42:05 -0500 } 7, 53 -- Received: by mail-il1-f177.google.com with SMTP id p16so3706194ilq.5 } 7, 53 -- for {microscopy-at-microscopy.com} ; Thu, 15 Oct 2020 04:53:16 -0700 (PDT) } 7, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 53 -- d=gmail.com; s=20161025; } 7, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 7, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 7, 53 -- bh=3Yei7pjUTFYggTdLpgupo2ZTJWUjRAG7l/W3FqyCK5s=; } 7, 53 -- b=EG9hvIuj5ExXVHj5a5BGxTC/ISPAhu0ZXbniJN6+kIRScPeGpNXiWIww+tvYBJKGy6 } 7, 53 -- nZMFxEQg3TCvC6ZxO2s0ThIuFKCSgHVw9W73zgvinYNDzhJSPmrTDE3+HTVrciO6ojy4 } 7, 53 -- TFp22WMoqk90An8lXc4axWn75E0g2n57PwDmJRsyFShj+DNGoYr5z/fNZ4slix/SVJv1 } 7, 53 -- 6TyBVlA7qD5mfbh84fw+sUILlheWlO8/KCSB9lOyYutsJgg5aJHPKtqGywfy+kZswphb } 7, 53 -- pIrS3bc+lleecOBghfvjckiaqg2AxBTQMJwED49awHWpTIX7F27rW5e3/V0Jel1H6Nhp } 7, 53 -- XOlw== } 7, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 7, 53 -- d=1e100.net; s=20161025; } 7, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 7, 53 -- :user-agent:mime-version:in-reply-to:content-language } 7, 53 -- :content-transfer-encoding; } 7, 53 -- bh=3Yei7pjUTFYggTdLpgupo2ZTJWUjRAG7l/W3FqyCK5s=; } 7, 53 -- b=Nd2aFAV/DHVmrfHRwJA7pGRdzcOMAhVAkGfXqL118SHg9zujV+wDzxc/ttre4OBAj2 } 7, 53 -- bV+sUctb12FtpsWvrb+LIO0PsGCxSW95u1NMcdFbIz8I3a6lxQ42QBIv5u7t8kyfFL5m } 7, 53 -- U837GVIEZcGHv8MbKuQwrvwjzYAyfq/fxk2lcWonGEhIfxcEv9C93csmm/gXyhxAP6zT } 7, 53 -- GBaeM9MiZIhdKYWvx27zcKHWaxc5LanxsZq97mOuspbad67DXZojMPFCULcEW7zk1xec } 7, 53 -- s66OLD/WSlvuPqexsOMAIExTPWxA/+8MsPH8kbMEZrGxaS4KmXHTQ5FyoMtQxxmED1hu } 7, 53 -- S8ng== } 7, 53 -- X-Gm-Message-State: AOAM531nIpbRI6+xp1OUtNam1Tn3tygEzz7S/g/MxNlgK4xCuxNfuDm6 } 7, 53 -- OdSsDNd+0bdKbrBgo7bcLRkFeSR6pomU+dTO } 7, 53 -- X-Google-Smtp-Source: ABdhPJxmtqM9Uyce5h46ANggDjMSJJ0mK/5Qxh/CVQQChAXdgGX9qA49M6AY8uUFX9HXRAxvV5thWg== } 7, 53 -- X-Received: by 2002:a92:ba8d:: with SMTP id t13mr2868019ill.131.1602762795653; } 7, 53 -- Thu, 15 Oct 2020 04:53:15 -0700 (PDT) } 7, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:d987:54be:4634:38b9]) } 7, 53 -- by smtp.googlemail.com with ESMTPSA id x14sm2310710ilg.21.2020.10.15.04.53.15 } 7, 53 -- for {microscopy-at-microscopy.com} } 7, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 7, 53 -- Thu, 15 Oct 2020 04:53:15 -0700 (PDT) } 7, 53 -- Subject: Fwd: Thin dense slice burning hole in carbon film during EBSD } 7, 53 -- References: {1462455C-C636-4AC5-A431-459C4A8C1565-at-otago.ac.nz} } 7, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 7, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 7, 53 -- X-Forwarded-Message-Id: {1462455C-C636-4AC5-A431-459C4A8C1565-at-otago.ac.nz} } 7, 53 -- Message-ID: {eee7e172-5986-16c9-4d02-5af615de4b4e-at-gmail.com} } 7, 53 -- Date: Thu, 15 Oct 2020 06:53:14 -0500 } 7, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) } 7, 53 -- Gecko/20100101 Thunderbird/68.12.1 } 7, 53 -- MIME-Version: 1.0 } 7, 53 -- In-Reply-To: {1462455C-C636-4AC5-A431-459C4A8C1565-at-otago.ac.nz} } 7, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 7, 53 -- Content-Language: en-US } 7, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
Is there any chance that your sample is hydrated or is reacting under the beam to release oxygen? Try another sample material like silicon and see if it still a problem. If it is, you might have a vacuum problem in your microscope -water or air leak.
If it is, it's reacting with the film and beam. I would try silicon nitride films. -Scott
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Thursday, October 15, 2020 7:44 AM To: s.walck-at-comcast.net
X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz}
Hi
We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB). The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we load a FIB produced slice mounted on a carbon film coated copper grid, the slice disappears within a short time of the beam going onto it. The hole remaining in the carbon film is the same shape as the original slice so I suspect that the slice is heating up and burning a hole in the film. The remainder of the film remains intact.
I would suggest using something like a SiN window grid rather than a carbon grid. They should be much more resistant to heating related problems.
On Thu, Oct 15, 2020 at 6:54 AM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz {mailto:allan.mitchell-at-otago.ac.nz} }
Hi
We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB). The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we load a FIB produced slice mounted on a carbon film coated copper grid, the slice disappears within a short time of the beam going onto it. The hole remaining in the carbon film is the same shape as the original slice so I suspect that the slice is heating up and burning a hole in the film. The remainder of the film remains intact.
Allan, Have you considered coating the whole sample? We use 1nm of Iridium for EBSD which works for most samples including fragile biological specimens. I don’t know how thick your “dense thin slice”is?
Bil Schneider SEM Lab Manager UW Madison Geosciences 608-333-7874
On Oct 15, 2020, at 6:57 AM, microscopy.listserver-at-gmail.com wrote:
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X-from: Allan Mitchell {allan.mitchell-at-otago.ac.nz}
Hi
We are trying to do EBSD (in an SEM) on a dense thin slice (produced by FIB). The thin slice is mounted on a carbon film, on a copper TEM grid. The problem is that each time we load a FIB produced slice mounted on a carbon film coated copper grid, the slice disappears within a short time of the beam going onto it. The hole remaining in the carbon film is the same shape as the original slice so I suspect that the slice is heating up and burning a hole in the film. The remainder of the film remains intact.
We were able to recover a Leica Cambridge S360 scanning electron microscope that worked for some time. For some reason the license diskette has been damaged. Would any of you have a copy of the SEM S360 license diskette V03.03 to send, borrow or sell?
Thanks for listening, Antonio.
==============================Original Headers============================== 3, 38 -- From acjoaquim-at-gmail.com Fri Oct 16 09:01:45 2020 3, 38 -- Received: from mail-ua1-f44.google.com (mail-ua1-f44.google.com [209.85.222.44]) 3, 38 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 09GE1jLt026828 3, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Oct 2020 09:01:45 -0500 3, 38 -- Received: by mail-ua1-f44.google.com with SMTP id x11so683571uav.1 3, 38 -- for {Microscopy-at-microscopy.com} ; Fri, 16 Oct 2020 07:12:59 -0700 (PDT) 3, 38 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 38 -- d=gmail.com; s=20161025; 3, 38 -- h=mime-version:from:date:message-id:subject:to; 3, 38 -- bh=s3DIsPVoZjykvKdvof34DbU9+pTl+oixquPnJs9v3lc=; 3, 38 -- b=aYd5UY0EUFGW839L/FmGDhOT8/RvhoqNhylDS5n59ZzwmPYg/6fw5zLKWbch9y99D5 3, 38 -- c78r5b+XrZn/ajfcclywypPMnsdqKIfNvdVU+3vnY/xahxNasnXTbhyFc0m2joTGZW+S 3, 38 -- o1uwlmO3Ii+r+aWk9YE/EMzeJ8T5gs0VX21+dYT0zSq7ih3tpWXSkX4lIT70roL2IlaM 3, 38 -- MInPBfWIOLH0B3L45hU578h/+0jO0J0jer8O6juUjBwlBl50FjSugQ/rPybv+SOG3z2+ 3, 38 -- +O6sYJi5EwqxwghX0oKDTH64OnPbp6FwfnYiOWkBEK09eubGC5XMuaNimQ2qEOVgtxMI 3, 38 -- tOZA== 3, 38 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 3, 38 -- d=1e100.net; s=20161025; 3, 38 -- h=x-gm-message-state:mime-version:from:date:message-id:subject:to; 3, 38 -- bh=s3DIsPVoZjykvKdvof34DbU9+pTl+oixquPnJs9v3lc=; 3, 38 -- b=KLv5u3FrjQ9gWuXuNUiyQ81Z3sMtjdfCcU6MjJJz2xDXxe70W/Kvfbg5QOydBveZ0R 3, 38 -- 3Lp6lv+tiDrS+BogsFpnyFg3db/rQ6oz0isu2gtY27ZYnjxfhvfbswtgLZG3PfyH3blu 3, 38 -- 930MhLk77yJxq7x1FpcJjBzDJqucxJwFWjSBxd6mPa+OrxH4ifwizP3C6M2pKo1CmmpL 3, 38 -- Rr+RyNVTkwKDKXDaOi46w3N9SZI8ltKl7fIqJXPhHl5r8JWrTwZaZrvZ/av7KW037skI 3, 38 -- Inajz76vXU4ZR9OPMEijBItuJSYJJHzxkdnfv8mx3ObQkUitD+ddVzvBsyNpaystAKNr 3, 38 -- gZ7g== 3, 38 -- X-Gm-Message-State: AOAM533JT7Je6MuMRSAdphz3kgEGmE1iuGcjkZbN7GmiqzrNBd3ukx37 3, 38 -- rJldZTUZbSOppSpkiUl11KFApK5y6AAGapNwES5cv3y4VqJhPQ== 3, 38 -- X-Google-Smtp-Source: ABdhPJwVPCN6ffD0TBmWBjG78z98OACE90tC4HTCu9xJzurQEeoWPA1q67/8WBbaZeABh+dyfqc5RdDrFyuUoe7TZBQ= 3, 38 -- X-Received: by 2002:ab0:20d5:: with SMTP id z21mr1867923ual.84.1602857578912; 3, 38 -- Fri, 16 Oct 2020 07:12:58 -0700 (PDT) 3, 38 -- MIME-Version: 1.0 3, 38 -- From: Antonio Carlos Joaquim {acjoaquim-at-gmail.com} 3, 38 -- Date: Fri, 16 Oct 2020 11:12:44 -0300 3, 38 -- Message-ID: {CAEK21K1C5ofz1NtJqe=h1Ba=wGTVGhCCirU03_jC18kxG-Tfhg-at-mail.gmail.com} 3, 38 -- Subject: SEM - Need help Cambridge S360 license diskette 3, 38 -- To: Microscopy-at-microscopy.com 3, 38 -- Content-Type: text/plain; charset="UTF-8" ==============================End of - Headers==============================
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Title-Subject: [Filtered] Using Methanol instead of Water in Single Particle Cryo-EM. Message: Hi,
Is there any one who try to use methanol in Cryo-EM instead of water? I just wonder because the vitrified water has 1000 kg/m3 density and the vitrified methanol has approximate 800 kg/m3 density. So if someone can put his/her sample inside methanol and can vitrified it, he/she can get a better contrast. Surroundings of the protein would be less dense So this can give better contrast to the protein. Of Course, there would be some issue using methanol. I am just asking this because of my curiosity. Best wishes, Sayit Ugurlu
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Title-Subject: [Filtered] Research Support Specialist position at the Electron Microscopy Resource Center
Message: Hi, there is an opening in my lab for a:
Research Support Specialist Electron Microscopy Resource Center The Rockefeller University 1230 York Avenue, New York, NY 10065
Rockefeller University is the worlds leading biomedical research University. Our groundbreaking discoveries in basic and clinical research are transforming medicine. We share a singular commitment to advancing science for the benefit of humanity. Our collaborative culture drives each of us to achieve a higher level, fueling the breakthroughs for which we are known. We seek a meticulous and detail-oriented Research Specialist to join our Electron Microscopy Resource Center (EMRC), who enjoys working in an intellectually rich and multi-disciplinary environment. Center Description
The Electron Microscopy Resource Center (EMRC) provides state-of-the-art electron microscopy support for analysis of a wide variety of biological samples, including viruses, bacteria, animal tissues as well as cultured cells and isolated cellular components for ultrastructural analyses or immuno-electron microscopy. The EMRC is equipped with two transmission electron microscopes, a conventional scanning electron microscope, and a high-pressure freezing and a freeze-substitution unit. (http://www.rockefeller.edu/emrc/)
Job Description Reporting to the Director of the EMRC, the Research Support Specialist will perform specimen preparation, including fixation, resin embedding, negative staining, ultrathin sectioning, and immunolabeling, operate the microscopes and associated equipment, train users, and consults with scientists on the design of experiments, data processing/analysis, interpretation of results, and informs users about the latest methodologies through familiarity with relevant literature. The Research Support Specialists will participate in all of EMRC daily operations, including maintenance, upkeep of the electron microscopes, associated equipment, and laboratory space, ordering supplies, and administrative tasks, including center billing. Qualifications
M.S. or Ph.D. degree or equivalent background in biology, bioengineering or a related field A minimum of five years of hands-on experience in electron microscopy, including biological sample preparation, ultramicrotomy, immuno-electron microscopy, image analysis, and interpretation Ability to produce high-quality EM samples and images Affinity for tasks requiring fine motor skills Strong communication skills and the ability to work collaboratively in a team as well as independently on a wide variety of research projects Ability to manage time efficiently, complete tasks on time, and to adapt to unscheduled requests Detail-oriented, focused, and highly motivated A strong background in computation and image acquisition/analysis programs (SerialEM, Image J) would be a plus
How to apply We offer a competitive salary, comprehensive benefits, and a collaborative working environment. Please visit the URL below and apply to the job code IRC25045. Please make sure to upload your resume and a cover letter: http://www2.rockefeller.edu/hr/jobs.php?url=https://recruit.rockefeller.edu/OA_HTML/OA.jsp?OAFunc=IRC_VIS_VAC_DISPLAY%26p_svid=25045%26p_spid=986026%26p_site_id=4361 Rockefeller University is an Equal Opportunity Employer - Minorities/Women/Disabled/Veterans
Hilda Amalia Pasolli, Ph.D. Director and Research Associate Professor Electron Microscopy Resource Center RRB 120F The Rockefeller University 1230 York Avenue, Box 230 New York, NY 10065
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I previously worked with methanol - it's possible... We were looking at block copolymer micelles in solution in methanol. Two points made it tricky: 1) The methanol evaporates very quickly compared to water to sample volumes and blotting times need to be experimented with for you specific sample, and 2) the boiling point of methanol is obviously much lower than water so you have to work very fast/low dose in the TEM so that your sample doesn't disappear in front of your eyes! Reference below....
Synthesis and Characterization of Amphiphilic Cyclic Diblock Copolypeptoids from N-Heterocyclic Carbene-Mediated Zwitterionic Polymerization of N-Substituted N-Carboxyanhydride
Thomas
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Saturday, October 17, 2020 8:34 AM To: Smart, Thomas Philip {tsmart-at-eastman.com}
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Title-Subject: [Filtered] Using Methanol instead of Water in Single Particle Cryo-EM. Message: Hi,
Is there any one who try to use methanol in Cryo-EM instead of water? I just wonder because the vitrified water has 1000 kg/m3 density and the vitrified methanol has approximate 800 kg/m3 density. So if someone can put his/her sample inside methanol and can vitrified it, he/she can get a better contrast. Surroundings of the protein would be less dense So this can give better contrast to the protein. Of Course, there would be some issue using methanol. I am just asking this because of my curiosity. Best wishes, Sayit Ugurlu
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The important quantity for phase contrast TEM is the electrostatic potential, not the density. You'd have to check how much the mean inner potential of water and methanol differ to see how much contrast you gain. Then there's also the biological question. Is the protein functional in methanol and does it keep its native conformation?
It's certainly an interesting idea, in any case.
Philip ________________________________________ Frn: tsmart-at-eastman.com {tsmart-at-eastman.com} Skickat: den 17 oktober 2020 18:35 Till: Philip Kck mne: [Microscopy] RE: [EXTERNAL] viaWWW: Using Methanol instead of Water
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Good morning Sayit,
I previously worked with methanol - it's possible... We were looking at block copolymer micelles in solution in methanol. Two points made it tricky: 1) The methanol evaporates very quickly compared to water to sample volumes and blotting times need to be experimented with for you specific sample, and 2) the boiling point of methanol is obviously much lower than water so you have to work very fast/low dose in the TEM so that your sample doesn't disappear in front of your eyes! Reference below....
Synthesis and Characterization of Amphiphilic Cyclic Diblock Copolypeptoids from N-Heterocyclic Carbene-Mediated Zwitterionic Polymerization of N-Substituted N-Carboxyanhydride
Thomas
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Saturday, October 17, 2020 8:34 AM To: Smart, Thomas Philip {tsmart-at-eastman.com}
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Title-Subject: [Filtered] Using Methanol instead of Water in Single Particle Cryo-EM. Message: Hi,
Is there any one who try to use methanol in Cryo-EM instead of water? I just wonder because the vitrified water has 1000 kg/m3 density and the vitrified methanol has approximate 800 kg/m3 density. So if someone can put his/her sample inside methanol and can vitrified it, he/she can get a better contrast. Surroundings of the protein would be less dense So this can give better contrast to the protein. Of Course, there would be some issue using methanol. I am just asking this because of my curiosity. Best wishes, Sayit Ugurlu
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Just my cents: It is an interesting question but as always with science, the first question to ask is: Does it make any sense?
What I mean is, the main reason Cryo-EM was developed is to keep the system as native as possible and thus avoid the artifacts coming from preparing the material in artificial conditions. Since most biological systems swim in some sort of watery soup, water seems to be the optimal medium for cryo-EM, not because the properties of water are optimal for observation in TEM but because it is the most meaningful medium.
Regards, Stephane
On Sunday, October 18, 2020, 09:15:52 AM GMT+2, koeck-at-kth.se {koeck-at-kth.se} wrote:
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Hi.
The important quantity for phase contrast TEM is the electrostatic potential, not the density. You'd have to check how much the mean inner potential of water and methanol differ to see how much contrast you gain. Then there's also the biological question. Is the protein functional in methanol and does it keep its native conformation?
It's certainly an interesting idea, in any case.
Philip ________________________________________ Från: tsmart-at-eastman.com {tsmart-at-eastman.com} Skickat: den 17 oktober 2020 18:35 Till: Philip Köck Ämne: [Microscopy] RE: [EXTERNAL] viaWWW: Using Methanol instead of Water
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Good morning Sayit,
I previously worked with methanol - it's possible... We were looking at block copolymer micelles in solution in methanol. Two points made it tricky: 1) The methanol evaporates very quickly compared to water to sample volumes and blotting times need to be experimented with for you specific sample, and 2) the boiling point of methanol is obviously much lower than water so you have to work very fast/low dose in the TEM so that your sample doesn't disappear in front of your eyes! Reference below....
Synthesis and Characterization of Amphiphilic Cyclic Diblock Copolypeptoids from N-Heterocyclic Carbene-Mediated Zwitterionic Polymerization of N-Substituted N-Carboxyanhydride
Thomas
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Saturday, October 17, 2020 8:34 AM To: Smart, Thomas Philip {tsmart-at-eastman.com}
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Title-Subject: [Filtered] Using Methanol instead of Water in Single Particle Cryo-EM. Message: Hi,
Is there any one who try to use methanol in Cryo-EM instead of water? I just wonder because the vitrified water has 1000 kg/m3 density and the vitrified methanol has approximate 800 kg/m3 density. So if someone can put his/her sample inside methanol and can vitrified it, he/she can get a better contrast. Surroundings of the protein would be less dense So this can give better contrast to the protein. Of Course, there would be some issue using methanol. I am just asking this because of my curiosity. Best wishes, Sayit Ugurlu
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==============================Original Headers============================== 7, 53 -- From microscopy.listserver-at-gmail.com Sat Oct 17 07:26:30 2020 7, 53 -- Received: from mail-io1-f47.google.com (mail-io1-f47.google.com [209.85.166.47]) 7, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 09HCQUTa025475 7, 53 -- for {microscopy-at-microscopy.com} ; Sat, 17 Oct 2020 07:26:30 -0500 7, 53 -- Received: by mail-io1-f47.google.com with SMTP id u19so7332806ion.3 7, 53 -- for {microscopy-at-microscopy.com} ; Sat, 17 Oct 2020 05:37:48 -0700 (PDT) 7, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 53 -- d=gmail.com; s=20161025; 7, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version 7, 53 -- :in-reply-to:content-language:content-transfer-encoding; 7, 53 -- bh=/v416V+8SZB1NhjnB6QEJWBcU/cMiRFzfxGhlWi7JtA=; 7, 53 -- b=NvqPqgnAGLjrmAhrGdi75MipgFzlG0IpHM6gHDq8flnL1SIDHddvzo3XDSSsEU01xX 7, 53 -- Y5r5EH+qzFijFOneP+rXl92sibanFA7v+5gX9P3u/EfNo8d5fE3SPSBZOBr8XEXsrUts 7, 53 -- K+YezQ6ZWlGbgopaz1nZXjRT8q9XjDWUQnMlaYP8tGDuP8AR+VlN0Q2o/AkpyK2P6GkB 7, 53 -- VOodyptyn8mtUBoXG6uPDUJF38z74Q4Ggk1O6e9mLjNcoFTKbC8AG79uuPlu1/StIzdI 7, 53 -- bC+iP+W6USbnbzTH6n25+8KutMl6mQpPykGZRXYRsUjnTm3Ybh2s+d5UInis5JdZ2sI5 7, 53 -- z/DQ== 7, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 7, 53 -- d=1e100.net; s=20161025; 7, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date 7, 53 -- :user-agent:mime-version:in-reply-to:content-language 7, 53 -- :content-transfer-encoding; 7, 53 -- bh=/v416V+8SZB1NhjnB6QEJWBcU/cMiRFzfxGhlWi7JtA=; 7, 53 -- b=XHkF3qQJXJJ3KHmauMVLabVLb3Iu/iuWk3oYvzp9WzXTu+9R6JKUiQUbpsUS3jFjRz 7, 53 -- 0OrhhK1NldXoQQajnVAz0Yr/FMR6CGrIWzA47YnRWylN7+rnX/HKz1vHLaKSF70tWRek 7, 53 -- oJ1W9IxqM/UfvOA8JLbmQNtrvdVTnmiDh7TR5v1N/yxQZZhxXvXe5ZrQp9H8haJrs919 7, 53 -- +Kyg6Z5Thy2VwY/hua+Q2t82oluzOx7EZK1nXzkaE6j/Mm3u1EGpiB7s6L59TfOXfHbZ 7, 53 -- F62EQ4xfM5vUTKhAT+bnrSkgd0qhlhe9xdWmFbupZHq83qVUmvWNdYNQ8iku/1DfcVd3 7, 53 -- IBaw== 7, 53 -- X-Gm-Message-State: AOAM5318ZinsJMkLBfRXPyvQQekENNHndrWgKRFCKXyHiU41yiccRfZm 7, 53 -- Acm9NuLmMAW4MJtkCmFy5PSNIu+uITKu+A== 7, 53 -- X-Google-Smtp-Source: ABdhPJz/7cfEqrt58X87Xan6v2VtJ0+98j+QqdwW+H8+3l7NEPpLLDNJ9mys7c9k2ZdyE7Y5k9l+Pw== 7, 53 -- X-Received: by 2002:a05:6602:2fc2:: with SMTP id v2mr5433383iow.19.1602938267383; 7, 53 -- Sat, 17 Oct 2020 05:37:47 -0700 (PDT) 7, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:6c35:e3b5:ba72:fdca]) 7, 53 -- by smtp.googlemail.com with ESMTPSA id o72sm5572255ilb.6.2020.10.17.05.37.45 7, 53 -- for {microscopy-at-microscopy.com} 7, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); 7, 53 -- Sat, 17 Oct 2020 05:37:46 -0700 (PDT) 7, 53 -- Subject: viaWWW: Using Methanol instead of Water in Single Particle Cryo-EM. 7, 53 -- References: {202010161246.09GCkoAp018230-at-microscopy.com} 7, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} 7, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} 7, 53 -- X-Forwarded-Message-Id: {202010161246.09GCkoAp018230-at-microscopy.com} 7, 53 -- Message-ID: {ca6be784-1777-1c97-795f-b642556e36d9-at-gmail.com} 7, 53 -- Date: Sat, 17 Oct 2020 07:37:45 -0500 7, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:68.0) 7, 53 -- Gecko/20100101 Thunderbird/68.12.1 7, 53 -- MIME-Version: 1.0 7, 53 -- In-Reply-To: {202010161246.09GCkoAp018230-at-microscopy.com} 7, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed 7, 53 -- Content-Language: en-US 7, 53 -- Content-Transfer-Encoding: 7bit ==============================End of - Headers==============================
Thank you for your input. It is a very good question (does it make sense?) but I think it does. After all, we use cryo because we cannot observe those systems in their native state. Adding an extra step (methanol) may bring us further away from native conditions but may also enable us to observe features that may not be visible in water. There is always the caveat that those features could be caused by the methanol.
Regards, Stefano
X-from: nizets2-at-yahoo.com {nizets2-at-yahoo.com} Sent: Monday, October 19, 2020 07:44 AM To: Stefano Rubino {stefano-at-soquelec.com}
Hi!
Just my cents: It is an interesting question but as always with science, the first question to ask is: Does it make any sense?
What I mean is, the main reason Cryo-EM was developed is to keep the system as native as possible and thus avoid the artifacts coming from preparing the material in artificial conditions. Since most biological systems swim in some sort of watery soup, water seems to be the optimal medium for cryo-EM, not because the properties of water are optimal for observation in TEM but because it is the most meaningful medium.
Regards, Stephane
On Sunday, October 18, 2020, 09:15:52 AM GMT+2, koeck-at-kth.se {koeck-at-kth.se} wrote:
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Hi.
The important quantity for phase contrast TEM is the electrostatic potential, not the density. You'd have to check how much the mean inner potential of water and methanol differ to see how much contrast you gain. Then there's also the biological question. Is the protein functional in methanol and does it keep its native conformation?
It's certainly an interesting idea, in any case.
Philip ________________________________________ Frn: tsmart-at-eastman.com {tsmart-at-eastman.com} Skickat: den 17 oktober 2020 18:35 Till: Philip Kck mne: [Microscopy] RE: [EXTERNAL] viaWWW: Using Methanol instead of Water
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America
Good morning Sayit,
I previously worked with methanol - it's possible... We were looking at block copolymer micelles in solution in methanol. Two points made it tricky: 1) The methanol evaporates very quickly compared to water to sample volumes and blotting times need to be experimented with for you specific sample, and 2) the boiling point of methanol is obviously much lower than water so you have to work very fast/low dose in the TEM so that your sample doesn't disappear in front of your eyes! Reference below....
Synthesis and Characterization of Amphiphilic Cyclic Diblock Copolypeptoids from N-Heterocyclic Carbene-Mediated Zwitterionic Polymerization of N-Substituted N-Carboxyanhydride
Thomas
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Saturday, October 17, 2020 8:34 AM To: Smart, Thomas Philip {tsmart-at-eastman.com}
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Title-Subject: [Filtered] Using Methanol instead of Water in Single Particle Cryo-EM. Message: Hi,
Is there any one who try to use methanol in Cryo-EM instead of water? I just wonder because the vitrified water has 1000 kg/m3 density and the vitrified methanol has approximate 800 kg/m3 density. So if someone can put his/her sample inside methanol and can vitrified it, he/she can get a better contrast. Surroundings of the protein would be less dense So this can give better contrast to the protein. Of Course, there would be some issue using methanol. I am just asking this because of my curiosity. Best wishes, Sayit Ugurlu
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Are there any very old-timers still around who can provide information about the differences between an RCA EMU-2B and an EMU-2D. OSU’s microscope that was donated to a local museum has always been described as an EMU-2B in the EM facility history; but I note that the manufacturer’s label says EMU-2D (Serial No. 775, MI-12957-E).
I seem to remember an seeing an article in Microscopy Today that dealt with the early history of the EMU series, but I haven’t found it yet.
Thanks for your memories!
Michael Nesson, Ph.D. ALS 2011 Dept Biochem/Biophys Oregon State University Corvallis OR
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I wondered if any of you already tried to detect viral particles in urine using TEM or SEM? Clearly one has to isolate somehow the particles from the soup. But perhaps I don't have to reinvent the wheel? I found many papers using different kinds of chromatography but before I try all of them, maybe I can rely on personal experience from my fellows here?
From smithdiana157-at-gmail.com Tue Oct 20 06:12:33 2020 Return-Path: {smithdiana157-at-gmail.com} Received: from gmail.com ([104.148.61.172]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 09KBCWlo024303 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 20 Oct 2020 06:12:33 -0500 Message-ID: {94D6A519.EA64F81B-at-gmail.com}
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Email: amini-at-rowan.edu Name: Shahram Amini
Title-Subject: [Filtered] Looking for a used or demo EDS
Message: Hello colleagues, We are in the process of purchasing a used ZEISS Supra 35 Variable Pressure FESEM but it doesn't have EDS capability on it. We are interested in buying a demo or used EDS for our new microscope. If you have any leads please kindly let me know. Thanks a lot. Regards, Shahram samini-at-pulsetechnologies.com amini-at-rowan.edu 215-510-9589
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Dear colleagues We would like to invite you to participate in virtual school "AI for atoms: How to machine learn STEM" to be held at ORNL, December 7-10, 2020 organized by Maxim Ziatdinov, Rama Vasudevan, Debangshu Mukherjee, and Sergei V. Kalinin. The school will feature the combination of invited and contributed talks and hands-on tutorials for AtomAI, GPim, and PyCroscopy packages. If interested, please register at the https://www.surveymonkey.com/r/257WD3B and contact Sergei Kalinin (sergei2-at-ornl.gov) to submit the abstract by November 1. The first call for the school is provided below, and looking forward to (virtually) meeting you at Oak Ridge! Sergei V. Kalinin
First call: Machine learning (ML) has emerged as a powerful tool for data and image analysis and as an enabling component of autonomous systems in areas ranging from biological and medical imaging to self-driving cars. This rapid growth in ML applications poses the question as to which of these methods can be applied in electron microscopy, and perhaps more importantly, what insights into the physics and chemistry of real materials can they yield. This virtual school on AI for atoms: how to machine learn STEM, to be held December 7-10, will combine invited and contributed presentations at the forefront of ML applications in Scanning Transmission Electron Microscopy (STEM), Electron Energy Loss Spectroscopy (EELS), and 4D STEM, as well as for physics and chemistry extraction from STEM data sets. It will further feature tutorials on recent developments in ML analysis of mesoscopic and atomically resolved images and spectroscopy in STEM, including classical graph analysis of STEM data, deep convolutional neural networks (DCNNs) for feature identifications, symmetry-invariant (variational) autoencoders ((V)AE), and Gaussian Processes based super-resolution imaging and image reconstruction. The tutorials will be followed by the hands-on tutorial sessions introducing the attendees to the AtomAI (https://github.com/pycroscopy/atomai), GPim (https://github.com/ziatdinovmax/GPim), and various Pycroscopy (https://github.com/pycroscopy/pycroscopy and https://www.github.com/pycroscopy/stemtool/) packages. All the technologies and workflows discussed during the tutorials will be open source. The attendees are encouraged to contact the organizers in advance to setup analysis of own datasets. The meeting will be free of charge. The final program will be available by November 7 and registration deadline is November 13.
From wandaver2m-at-gmail.com Thu Oct 22 01:47:49 2020 Return-Path: {wandaver2m-at-gmail.com} Received: from gmail.com ([104.148.61.171]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 09M6lmb5003637 for {microscopylistserverarchive7-at-microscopy.com} ; Thu, 22 Oct 2020 01:47:49 -0500 Message-ID: {CB44E4D4.7E285E50-at-gmail.com}
Third International Symposium on Advanced Microscopy and Spectroscopy on the Occasion of Celebrating the NSF MRSEC at UCI University of California, Irvine
November 5-7, 2020
To celebrate the establishment of the Center for Complex and Active Materials (CCAM) - a new Materials Research Science and Engineering Center (MRSEC) funded by National Science Foundation (NSF), UCI Irvine Materials Research Institute (IMRI) is delighted to host a special materials research workshop in conjunction with the Third International Symposium on Advanced Microscopy and Spectroscopy (ISAMS-3), on November 5-7, 2020. A workshop centered on CCAM activities will be held on November 5, to kick off the UCI MRSEC, followed by ISAMS-3 on November 6-7, 2020. The ISAMS-3 will bring together the scientific community working on various aspects of transmission electron microscopy to exchange latest research results and ideas, and to address challenges in the development of atomic scale imaging and spectroscopy for advancing materials and biological sciences. Both events will be held virtually via Zoom and are open to the scientific community. For more information about the events, please visit: https://sites.uci.edu/isams3/.
Register here at https://sites.uci.edu/isams3/#Registration, A Zoom link will be sent to all registered attendees by email.
The National Science Foundation has awarded $18 million to UCI in support of the CCAM - a new NSF MRSEC. The CCAM builds on campus strengths in multidisciplinary science and engineering research, world-class facilities, and commitment to diversity. The primary mission of CCAM is to establish foundational knowledge in the science and engineering of new classes of materials offering unique and broad functionality via an interplay among design, simulation, synthesis, and advanced characterization.
Established in 2015, UCI IMRI is an interdisciplinary special research program under the Office of Research, and a key enabler of the CCAM initiative. It serves as the cross-campus nexus for materials research in Southern California. IMRI operates a wide range of state-of-the-art shared facilities for the analysis and characterization of materials and devices ranging from sub- to macroscopic length scales. The facilities are professionally staffed, convenient, and affordable, with user-friendly services.
We are honored to invite you to join us at the special materials research workshop and the ISAMS-3, to share with you our accomplishments and to enable future collaborations amongst attendees.
Xiaoqing Pan Director, IMRI and CCAM - An NSF MRSEC
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Email: afm-at-stanford.edu Name: Ann Marshall
Title-Subject: [Filtered] Stanford EM-X Webinar Series
Message: We are excited to announce the launching of the Stanford Electron Microscopy-X (EM-X) Symposium series. The organizing co-chairs are Professors Robert Sinclair, Wah Chiu, Jennifer Dionne and Yi Cui at Stanford University/SLAC. We would like to invite you to attend this online event.
Time: Nov. 2 (Monday), 8-9:30am California time. Speakers: Professor Jacques Dubochet (2017 Nobel Laureate in Chemistry) University of Lausanne Talk title"A Nobel prize, how and what for?" Professor Yi Cui (2017 Blavatnik Laureate in Physical Sciences and Engineering) Stanford University/SLAC National Lab Talk title"Cryo and in-situ electron microscopy for clean energy" Please register at: https://stanford.zoom.us/webinar/register/WN_-ELnXVi1S1qgE9PlaIGeDw
The EM-X event is in on-line format. It will be delivered Iive". Each presentation is 30 min plus 15 min Q&A, with a possible panel discussion at the end. We plan to hold this symposium series monthly. Each event will usually consist of two speakers (one in the biomedical area and the other in physical science and engineering). Here we use "X" to indicate the breadth of EM technique development and the many rich scientific problems EM can address. Due to the COVID-19 situation, we believe that it is timely to initiate such a symposium series to bring the global electron microscopy community together, to highlight exciting progress and to stimulate creative thinking for future developments. We invite attendees internationally from academia, national labs, industry, funding agencies and manufacturers. Ann Marshall TEM Manager Stanford Nano Shared Facilities
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The Canadian Centre for Electron Microscopy (CCEM), located at McMaster University in Hamilton, ON, Canada provides world-class electron microscopy capabilities and expertise to Canadian researchers and industry working in a broad range of fields. Our vision is to be one of the leading electron microscopy facilities in the world for the quality of the scientific research, to be the go-to provider of electron microscopy services to Canadian industry and to play a leading role in promoting interactions amongst researchers in various fields nationally and internationally.
The CCEM is seeking a NION microscope operator to join CCEM's research staff team. The Nion operator will be responsible for operations, trouble shooting and data acquisition of CCEM's Nion Hermes UltraSTEM transmission electron microscope which will be installed in early 2021. The position requires in-depth knowledge in the science and practical operation of the instrument, knowledge of the physics of monochromated low-loss electron energy loss spectroscopy as well as a strong background in data processing and hardware scripting using Python. Additionally, excellent skills in communicating and working collaboratively with team members and partners are required. This position reports to the Facilities Manager of the CCEM.
Assets: - Participation and leadership in scientific projects - Processing of data into scientific results - Development of custom acquisition and processing methods using the NION API and Python - Participate/lead publishing results in peer-reviewed journals - Expert knowledge in operating a Nion microscope - Acquisition and processing of monchromated electron energy loss spectroscopy data - Ability to troubleshoot and service the microscope with help of NION support - Keeping informed about new techniques and instrumentation - Dealing with vendors in consistent manner - Must be able to collaboratively work in a team and with external partners/customers - Must be able to plan concurrent projects effectively - Must be able to stay within the allocated resources (time, materials) for a given task - Represents CCEM's vision and mission in a positive way to partners and customers - Teaches operations and theory of microscopy to others - PhD in Physics, Engineering or related fields a strong asset - Strong track record of publications an asset Additional Information This position is initially limited to 2 years, with the possibility of renewal.
To apply for this position, please use the following link: https://careers.mcmaster.ca/psp/prepprd/EMPLOYEE/HRMS/c/HRS_HRAM.HRS_APP_SCHJOB.GBL?Page=HRS_APP_JBPST&Action=U&FOCUS=Applicant&SiteId=1001&JobOpeningId=34884&PostingSeq=1
Andreas Korinek,PhD Acting Executive Director Canadian Centre for Electron Microscopy 1280 Main St W ABB B161 Hamilton, ON, L8S 4M1 location:ABB B161 phone:+1 905 525 9140 x 20400 email:korinek-at-mcmaster.ca web:http://ccem.mcmaster.ca
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Email: ralvaradojr-at-ufl.edu Name: Rudy Alvarado
Title-Subject: [Filtered] HELP! Processing Spleen FFPE tissue for Immunoelectron Microscopy
Message: Hi everyone, I am in need of help. I'm trying to process spleen tissue that was formalin-fixed paraffin embedded. I have processed this tissue twice, and I had the same results. The tissue get embedded by HM20 resin with Z6040 embedding primer, however, when I sectioned the tissue, it crumpled as if it was not properly infiltrated. To process the FFPE tissue, I excised 1-2 mm2 core punches from the FFPE blocks. I did extensive xylene washes (7-10 washes in 3-5 days) on a rocker. The tissue was reverse processed with the aid of a microwave processor: 5-6x 100% EtOH, 75% EtOH, 50% EtOH, 2x water washes, 1x PBS wash, fixed in 4% PFA/0.5% GA in PBS (overnight on shaker), 3-5x PBS washes, agarose encapsulated one of the samples (sample #1), PBS wash, 2x water washes, short EtOH dehydration series, one of the samples was en bloc treated in 2% UA in 75% EtOH (sample #2), 2x anhydrous EtOH washes, 50% resin (anhydrous acetone: HM20 resin), 6-10x 100% resin exchanges (during 5-7 days; no MW was used; tissue was kept in 4 deg C during the exchange on a shaker at 100 RPM). When I transferred the pieces to flat bottom capsules or Eppendorf tubes, I added some nitrogen gas to remove any oxygen present and then cap the tubes. I placed the samples in a freeze sub unit and uv cold cured at - 20 deg C for at least 48 hours. Lastly, I did use a new HM20 kit. I know this was overkill, but if anyone has any suggestions, I very much appreciate it. I successfully processed FFPE lung and intestine tissue before. Thank you, Rudy
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From fantjame060utg-at-gmail.com Tue Oct 27 06:49:54 2020 Return-Path: {fantjame060utg-at-gmail.com} Received: from gmail.com ([104.148.61.169]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 09RBnqPI006898 for {microscopylistserverarchive7-at-microscopy.com} ; Tue, 27 Oct 2020 06:49:53 -0500 Message-ID: {A68D20A3.DF9BF432-at-gmail.com}
Hi Rudy!
My suggestion would be to stop wasting time and resources making EM with FFPE samples. Formalin contains methanol and histology fixation leads to heavy artifacts like protein precipitation, sample shrinkage etc.
In EM you'll get a high resolution view of something very far from native state, what's the point? In short: you'll generate results but absolutely not meaningful.
My 2 cents
Best regards, Stephane
On Monday, October 26, 2020, 11:15:13 PM GMT+1, microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} wrote:
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Email: ralvaradojr-at-ufl.edu Name: Rudy Alvarado
Title-Subject: [Filtered] HELP! Processing Spleen FFPE tissue for Immunoelectron Microscopy
Message: Hi everyone, I am in need of help. I'm trying to process spleen tissue that was formalin-fixed paraffin embedded. I have processed this tissue twice, and I had the same results. The tissue get embedded by HM20 resin with Z6040 embedding primer, however, when I sectioned the tissue, it crumpled as if it was not properly infiltrated. To process the FFPE tissue, I excised 1-2 mm2 core punches from the FFPE blocks. I did extensive xylene washes (7-10 washes in 3-5 days) on a rocker. The tissue was reverse processed with the aid of a microwave processor: 5-6x 100% EtOH, 75% EtOH, 50% EtOH, 2x water washes, 1x PBS wash, fixed in 4% PFA/0.5% GA in PBS (overnight on shaker), 3-5x PBS washes, agarose encapsulated one of the samples (sample #1), PBS wash, 2x water washes, short EtOH dehydration series, one of the samples was en bloc treated in 2% UA in 75% EtOH (sample #2), 2x anhydrous EtOH washes, 50% resin (anhydrous acetone: HM20 resin), 6-10x 100% resin exchanges (during 5-7 days; no MW was used; tissue was kept in 4 deg C during the exchange on a shaker at 100 RPM). When I transferred the pieces to flat bottom capsules or Eppendorf tubes, I added some nitrogen gas to remove any oxygen present and then cap the tubes. I placed the samples in a freeze sub unit and uv cold cured at - 20 deg C for at least 48 hours. Lastly, I did use a new HM20 kit. I know this was overkill, but if anyone has any suggestions, I very much appreciate it. I successfully processed FFPE lung and intestine tissue before. Thank you, Rudy
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Title-Subject: [Filtered] size distribution for negatively stained exosomes.
Message: Hi, I am trying to measure the size distribution for the negatively stained exosome particles. But the background is unevenly stained, which makes it difficult to set the threshold. Can anyone please suggest any tool where I can select individual particles and perform the analysis? Login Host: 129.130.132.160 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I am looking for a protocol to critical-point-dry spirulina algae grown on glass substrates.
Normally I would go directly into 2% Glutaraldehyde for fixation.
Or would you recommend using a buffer first?
Next steps would be postfix with 2% OsO4, washing and the Ethanol up to 100%.
Any other recommendations?
Thanks,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Dear colleagues We would like to invite you to submit an abstract for the virtual school "AI for atoms: How to machine learn (S)TEM" to be held at ORNL, December 7-10, 2020 organized by Maxim Ziatdinov, Rama Vasudevan, Debangshu Mukherjee, and Sergei V. Kalinin. The contributed talks will be selected on the topics merging (S)TEM/EELS/4DSTEM with quantification and ML. The school will feature the combination of invited talks from leading experts in physics-applied machine learning and electron microscopy, including Paul Voyles (U Wisc), Viren Jain and Peter Battaglia (Google), Colin Ophus (Berkeley), Shirley Ho (Flatiron institute), Pinshane Huang (UIUC), A.G. Wilson (New York University) and contributed talks selected from submitted abstracts, as well as hands-on tutorials for AtomAI, GPim, and PyCroscopy packages. Please register at the https://www.surveymonkey.com/r/257WD3B. Submit the abstracts by November 3 via e-mail directly to Sergei Kalinin (sergei2-at-ornl.gov). The second call for the school is provided below, and looking forward to (virtually) meeting you at Oak Ridge! Sergei V. Kalinin
Second call: Machine learning (ML) has emerged as a powerful tool for data and image analysis and as an enabling component of autonomous systems in areas ranging from biological and medical imaging to self-driving cars. This rapid growth in ML applications poses the question as to which of these methods can be applied in electron microscopy, and perhaps more importantly, what insights into the physics and chemistry of real materials can they yield. This virtual school on AI for atoms: how to machine learn (S)TEM, to be held December 7-10, will combine invited and contributed presentations at the forefront of ML applications in Scanning Transmission Electron Microscopy (STEM) and Transmission Electron Microscopy (TEM), Electron Energy Loss Spectroscopy (EELS), and 4D STEM, as well as for physics and chemistry extraction from STEM data sets. The school will feature the combination of invited talks from leading experts in physics-applied machine learning and electron microscopy, including Paul Voyles (U Wisc), Viren Jain and Peter Battaglia (Google), Colin Ophus (Berkeley), Shirley Ho (Flatiron institute), Pinshane Huang (UIUC), A.G. Wilson (New York University) and contributed talks selected from submitted abstracts. It will further feature tutorials on recent developments in ML analysis of mesoscopic and atomically resolved images and spectroscopy in STEM, including classical graph analysis of STEM data, deep convolutional neural networks (DCNNs) for feature identifications, symmetry-invariant (variational) autoencoders ((V)AE), and Gaussian Processes based super-resolution imaging and image reconstruction. The tutorials will be followed by the hands-on tutorial sessions introducing the attendees to the AtomAI (https://github.com/pycroscopy/atomai), GPim (https://github.com/ziatdinovmax/GPim), and various Pycroscopy (https://github.com/pycroscopy/pycroscopy and https://www.github.com/pycroscopy/stemtool/) packages. All the technologies and workflows discussed during the tutorials will be open source. The a! ttendees are encouraged to contact the organizers in advance to setup analysis of own datasets. The meeting will be free of charge. The final program will be available by November 7 and registration deadline is November 13.
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Hello Ravi, It all depends on the quality of negative staining. What kind of support film did you use, carbon or carbon/formvar? Did you use a glow discharge? In our hands, ~1% ammonium molybdate with about 0.1% trehalose worked well on glow discharge treated carbon/formvar grids for negative staining of exosome particles. Ammonium molybdate produces lower contrast than uranyl salts, but it can make evenly spread negative stain.
Regards
Oldrich
-- Oldřich Benada Institute of Microbiology, Czech Acad. Sci. Laboratory of Molecular Structure Characterization Electron Microscopy Group Vídeňská 1083 142 20 Prague 4 Czech Republic
On Thu, 29 Oct 2020 06:23:06 -0500, microscopy.listserver-at-gmail.com wrote : } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of } America To Subscribe/Unsubscribe -- } http://www.microscopy.com/MicroscopyListserver On-Line Help } http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } X-from: ravi.thakkar369-at-gmail.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/ } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when } replying please copy to both ravi.thakkar369-at-gmail.com, } Microscopy-at-Microscopy.com so that all Microscopy Listserver } Subscribers can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: ravi.thakkar369-at-gmail.com Name: Ravi } Title-Subject: [Filtered] size distribution for negatively stained } exosomes. } } Message: Hi, } I am trying to measure the size distribution for the negatively } stained exosome particles. But the background is unevenly stained, } which makes it difficult to set the threshold. Can anyone please } suggest any tool where I can select individual particles and perform } the analysis? 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==============================Original Headers============================== 10, 30 -- From benada-at-biomed.cas.cz Wed Nov 4 19:12:16 2020 10, 30 -- Received: from mail2.biomed.cas.cz (mail2.biomed.cas.cz [147.231.40.32]) 10, 30 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0A51CFgM003818 10, 30 -- for {microscopy-at-microscopy.com} ; Wed, 4 Nov 2020 19:12:16 -0600 10, 30 -- Received: from u117ob02 (nb170ph.mbu.cas.cz [147.231.44.133]) 10, 30 -- (using TLSv1.3 with cipher TLS_AES_256_GCM_SHA384 (256/256 bits) 10, 30 -- key-exchange ECDHE (P-256) server-signature RSA-PSS (2048 bits) server-digest SHA256) 10, 30 -- (No client certificate requested) 10, 30 -- by mail2.biomed.cas.cz (Postfix) with ESMTPSA id 9560AD00C4B; 10, 30 -- Mon, 2 Nov 2020 08:54:35 +0100 (CET) 10, 30 -- Date: Mon, 2 Nov 2020 08:54:34 +0100 10, 30 -- From: Oldrich Benada {benada-at-biomed.cas.cz} 10, 30 -- To: {microscopy-at-microscopy.com} 10, 30 -- Cc: ravi.thakkar369-at-gmail.com 10, 30 -- Subject: Re: [SPAM] [Microscopy] viaWWW:size distribution for negatively 10, 30 -- stained exosomes 10, 30 -- Message-ID: {20201102085434.3262d222-at-u117ob02} 10, 30 -- In-Reply-To: {202010291123.09TBN6WD004000-at-microscopy.com} 10, 30 -- References: {202010291123.09TBN6WD004000-at-microscopy.com} 10, 30 -- Organization: =?UTF-8?B?TWlrcm9iaW9sb2dpY2vDvSDDunN0YXY=?= AV 10, 30 -- =?UTF-8?B?xIxS?= 10, 30 -- X-Mailer: Claws Mail 3.17.3 (GTK+ 2.24.32; i686-pc-linux-gnu) 10, 30 -- MIME-Version: 1.0 10, 30 -- Content-Type: text/plain; charset=UTF-8 10, 30 -- X-IoP-CAS-MailScanner-Information: Please contact the ISP for more information 10, 30 -- X-IoP-CAS-MailScanner-ID: 9560AD00C4B.A9B9F 10, 30 -- X-IoP-CAS-MailScanner: Processed 10, 30 -- X-Spam-Status: No 10, 30 -- Content-Transfer-Encoding: 8bit 10, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 0A51CFgM003818 ==============================End of - Headers==============================
Our rather old (27 years and counting) 2010 TEM has developed a problem.
I came in yesterday and it had completely shut down.. Up until yesterday it had been working well. Checking the records, there were no power issues (we have a UPS plus generator backed up power), no cooling water issues, no gas (solenoid or insulating) issues, and no room temperature issues either. As it had completely shut down there were no alarms showing either. A quick investigation showed that the main circuit breaker on the PS had activated.
Resetting this enabled the TEM to restart, but after around 30 seconds the breaker activated again and the TEM shut down. But, the actual breaker switch doesn't drop fully to the open position. The only way that happens it to manually push the breaker down. I have tried a few times and always it shuts down, but the breaker never drops fully. Could it be a faulty breaker?
I was wondering if anyone out there has experienced a similar issue and may have any clues.
I realise that there may be a multitude of reasons, but any hint would be useful.
E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057 Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia. www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx
CSIRO acknowledges the Traditional Owners of the lands that we live and work on across Australia and pays its respect to Elders past, present and emerging.
PLEASE NOTE The information contained in this email may be confidential or privileged. Any unauthorised use or disclosure is prohibited. If you have received this email in error, please delete it immediately and notify the sender by return email. Thank you. To the extent permitted by law, CSIRO does not represent, warrant and/or guarantee that the integrity of this communication has been maintained or that the communication is free of errors, virus, interception or interference.
Please consider the environment before printing this email.
From fritschemichael755-at-gmail.com Fri Nov 6 06:52:39 2020 Return-Path: {fritschemichael755-at-gmail.com} Received: from gmail.com ([104.148.61.172]) by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 0A6CqcS5030151 for {microscopylistserverarchive7-at-microscopy.com} ; Fri, 6 Nov 2020 06:52:38 -0600 Message-ID: {2B9C7D75.C8E814D1-at-gmail.com}
We are a mineral isotope lab with a Zeiss EVO MA15 SEM (LaB6) used in a supporting role to characterize mineral compositional contrast (often very subtle) with backscattered electrons, normally at high vacuum, high kV, and beam currents up to 5 nA. BSE detection currently utilizes Zeiss' 4 quadrant semiconductor detector under the pole piece. I'm interested to hear from those who have experience using a YAG scintillator detector for similar purposes, either the one made by Zeiss or others, and particularly if you've any evidence of their relative sensitivity to compositional contrast. I'm led to believe that the YAG detector is more sensitive than the semiconductor type at the analytical conditions mentioned above (high vacuum, high kV). What do the users have to say? Do any publications exist in which the YAG detector performance is objectively evaluated?
Thanks and best regards,
Richard Stern Managing Director, CCIM (SIMS facility) Department of Earth & Atm Sciences University of Alberta 116 St. & 85 Ave. Edmonton, AB T6G 2R3 Canada
780-248-1063 (lab)
==============================Original Headers============================== 6, 29 -- From rstern-at-ualberta.ca Sun Nov 8 15:27:05 2020 6, 29 -- Received: from smtp-03.srv.ualberta.ca (smtp-03.srv.ualberta.ca [129.128.162.23]) 6, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0A8LR5eD006272 6, 29 -- for {microscopy-at-microscopy.com} ; Sun, 8 Nov 2020 15:27:05 -0600 6, 29 -- Received: from T580RSTERN (t580-rstern.eas.ualberta.ca [129.128.30.109]) 6, 29 -- by smtp-03.srv.ualberta.ca (Postfix) with ESMTPSA id 2AAC8D7DC8 6, 29 -- for {microscopy-at-microscopy.com} ; Sun, 8 Nov 2020 14:39:35 -0700 (MST) 6, 29 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; d=ualberta.ca; 6, 29 -- s=smtp-20200808; t=1604871575; 6, 29 -- bh=2Tb64VjVi/r/Bb1IcLsoGwhKlSavQ47hrkiWKhaPn3g=; 6, 29 -- h=From:To:Subject:Date:Message-ID:From:To:Subject; 6, 29 -- b=DCDAnFhx2LuJJlHMxijp8gExGSWWdZL7jtbmXtQd93Bw5gxbfEkqCDYLIG0qR/gsv 6, 29 -- mGg9sqFW3XHixQWvlp92JzkY1WCO8nCeIXh0Y9mrAxHq9yMWUsNq5RdPBWWlNZk/KL 6, 29 -- n9V3IKcsKhMJEnFYqakRrxf4q+2ePkriTykZIov/Y3fAJdXeOhjikMhgbHmaRFZTnB 6, 29 -- 9WPjhgxW2GGHFRFkm1LvvPiGw1clqVcq2NRHuAr1lUqtnKKdI3yiPMJoZVkzbZpffV 6, 29 -- b80XYOHGE20oSzA5zXf6+V9G3E/eF3PK/c4ZMVTnbJJWdzhkpJcMpZKurUWHT6xE36 6, 29 -- Y4mba+m3PZSgw== 6, 29 -- From: "Richard Stern" {rstern-at-ualberta.ca} 6, 29 -- To: {microscopy-at-microscopy.com} 6, 29 -- Subject: SEM-scintillator vs. semiconductor BSE detector performance 6, 29 -- Date: Sun, 8 Nov 2020 14:41:28 -0700 6, 29 -- Message-ID: {005701d6b617$ea5e4d00$bf1ae700$-at-ualberta.ca} 6, 29 -- MIME-Version: 1.0 6, 29 -- Content-Type: text/plain; 6, 29 -- charset="us-ascii" 6, 29 -- Content-Transfer-Encoding: 7bit 6, 29 -- X-Mailer: Microsoft Outlook 14.0 6, 29 -- Thread-Index: Ada2Fu2d8QvChTeLQguV8fhHUEMQhw== 6, 29 -- Content-Language: en-ca ==============================End of - Headers==============================
at a friend`s Quanta 200 FEG-SEM a message is shown:
"Communication Problems with controller PUC"
and the TAD test shows at "CAN Tests" a problem with FGSU and VCB.
Anyone out there who knows if this is a hardware problem or more a software problem?
Even after a cold restart the scope comes back with the same errors.
Best wishes,
Stefan
--
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Dear colleagues - as a reminder, this Thursday-Friday are the last days to register for the virtual school "AI for atoms: How to machine learn STEM" to be held at ORNL, December 7-10. The registration site is https://www.surveymonkey.com/r/257WD3B.
Please contact Sergei Kalinin (sergei2-at-ornl.gov) to submit the abstract. The tentative program is provided below. Given the recent progress in the field, the open invited and contributed talks will be selected from submitted abstracts.
Looking forward to (virtually) meeting you at Oak Ridge!
Sergei
Tentative program
December 7. 9.30 am - 9.45 am Opening presentation and workshop plan 9.45 am - 10.30 am, Max Tegmark (MIT), TBA 10.30 am - 11.00 am, Paul Voyles (U. Wisc.) Supervised and Unsupervised Machine Learning for 4D STEM Data 11.00 am - 11.30 am, Pinshane Huang (U. Illinois) Probing the Strain Fields of Single-Atom Defects via Deep Learning 11.30 am - 11.45 am Contributed talk 1 11.45 am - 12.00 am Contributed talks 2 12.00 am - 12.30 pm Peter Battaglia (Google) Learning simulation using structured models
1.30 pm - 4.00 pm Tutorial 1: Intro and semantic segmentation of images * Intro to NN in PyTorch: Regression * Intro to NN in PyTorch: Image-level classification (+ visualization of activation maps, etc.) * Semantic segmentation: a basic example in Pytorch and transition to AtomAI.
December 8 10.00 am - 10.30 am, Colin Ophus (Berkeley) Computational Imaging, data analysis, and machine learning in STEM and TEM 10.30 am - 11.00 am, Shirley Ho (Flatiron Institute) Discovering symbolic equations using deep learning 11.00 am - 11.30 am, Andrew G. Wilson, (NYU) Learning Symmetries in Neural Networks 11.30 am - 11.45 am Contributed talk 1 11.45 am - 12.00 am Contributed talks 2 1200 pm - 12.30 pm Invited talk
1.30 pm - 4.00 pm Tutorial 2: Autoencoders * Autoencoders for data cleaning * Im2spec encoder-decoder networks * Variational and invariant autoencoders
December 9 10.00 am - 10.30 am Vipin Kumar (UMN), Physics-Guided Machine Learning: A New Framework for Accelerating Scientific Discovery 10.30 am - 11.00 am Magnus Nord (NTNU) Processing very large datasets using pyXem: STEM-DPC of ferromagnetic domains 11.00 am - 11.30 am, Gerd Duscher (UTK) How can STEM be quantitative? 11.30 am - 11.45 am Contributed talk 1 11.45 am - 12.00 am Contributed talks 2 1200 pm - 12.30 pm Invited talk
December 10 10.00 am - 10.30 am Robert Howden, Maximum resolution from the Ronchigram: human vs. deep learning 10.30 am - 11.00 am Martin Jankowiak (Broad Institute) Bayesian methods for adaptive experimental design 11.00 am - 11.30 am, Dhiresha Kudithipudi (UTSA), Task Agnostic Lifelong Learning 11.30 am - 11.45 am Contributed talk 1 11.45 am - 12.00 am Contributed talk 2 12.00 pm - 12.30 pm Viren Jain (Google) Synapse-resolution connectomics in fly, bird, and human brains
Thank you all for your suggestions on possible causes for the circuit breaker opening on my 2010.
The problem has been resolved and was relatively straightforward.
The facilities people (unknown to me) turned the temperature of the cooling water down to around 16 degrees C. This was below the dew point of the lab for a few vey humid days. Water condensed onto the diffusion pump (and other places) and from there dripped into the diffusion pump heater shield. This caused a short which threw the breaker when it was powered up (~ 30 s after initial start-up).
The cooling water temperature has been changed and all the parts dried out. It is now back on line and running fine.
E colin.veitch-at-csiro.au T +61 3 5246 4891 M 0438 538 475 F +61 3 5246 4057 Address CSIRO Manufacturing, 75 Pigdons Road, Waurn Ponds, Vic 3216, Australia. www.csiro.au | http://www.csiro.au/Organisation-Structure/Flagships/Manufacturing.aspx
CSIRO acknowledges the Traditional Owners of the lands that we live and work on across Australia and pays its respect to Elders past, present and emerging.
PLEASE NOTE The information contained in this email may be confidential or privileged. Any unauthorised use or disclosure is prohibited. If you have received this email in error, please delete it immediately and notify the sender by return email. Thank you. To the extent permitted by law, CSIRO does not represent, warrant and/or guarantee that the integrity of this communication has been maintained or that the communication is free of errors, virus, interception or interference.
Please consider the environment before printing this email.
Hi,
Our rather old (27 years and counting) 2010 TEM has developed a problem.
I came in yesterday and it had completely shut down.. Up until yesterday it had been working well. Checking the records, there were no power issues (we have a UPS plus generator backed up power), no cooling water issues, no gas (solenoid or insulating) issues, and no room temperature issues either. As it had completely shut down there were no alarms showing either. A quick investigation showed that the main circuit breaker on the PS had activated.
Resetting this enabled the TEM to restart, but after around 30 seconds the breaker activated again and the TEM shut down. But, the actual breaker switch doesn't drop fully to the open position. The only way that happens it to manually push the breaker down. I have tried a few times and always it shuts down, but the breaker never drops fully. Could it be a faulty breaker?
I was wondering if anyone out there has experienced a similar issue and may have any clues.
I realise that there may be a multitude of reasons, but any hint would be useful.
X-from: Frank Karl {frank_karl-at-ardl.com} Hello Everyone, I have just used my last 0-ring on the sample rod for our FEI CM12. We are no longer covered by their service contract and I need to find a replacement source. I thought I had a life time supply, but it seems I'm wrong. I believe the O-ring is Viton but their 11 digit number doesn't mean anything outside of the FEI walls. Does anyone have any information on size, perhaps a catalog number for a company from which I can order from? Thanks!!!
Stay calm...Be brave....watch for signs
Frank Karl Microscopist Akron Rubber Development Laboratory 2887 Gilchrist Road Akron, Ohio 44305
Dear colleagues - as a reminder, this Thursday-Friday are the last days to register for the virtual school "AI for atoms: How to machine learn STEM" to be held at ORNL, December 7-10. The registration site is https://www.surveymonkey.com/r/257WD3B. Please contact Sergei Kalinin (sergei2-at-ornl.gov) to submit the abstract. The tentative program is provided below. Given the recent progress in the field, the open invited and contributed talks will be selected from submitted abstracts. Looking forward to (virtually) meeting you at Oak Ridge!
Sergei
Tentative program
December 7. 9.30 am - 9.45 am Opening presentation and workshop plan 9.45 am - 10.30 am, Max Tegmark (MIT), TBA 10.30 am - 11.00 am, Paul Voyles (U. Wisc.) Supervised and Unsupervised Machine Learning for 4D STEM Data 11.00 am - 11.30 am, Pinshane Huang (U. Illinois) Probing the Strain Fields of Single-Atom Defects via Deep Learning 11.30 am - 11.45 am Contributed talk 1 11.45 am - 12.00 am Contributed talks 2 12.00 am - 12.30 pm Peter Battaglia (Google) TBA 1.30 pm - 4.00 pm Tutorial 1: Intro and semantic segmentation of images * Intro to NN in PyTorch: Regression * Intro to NN in PyTorch: Image-level classification (+ visualization of activation maps, etc.) * Semantic segmentation: a basic example in Pytorch and transition to AtomAI.
December 8 10.00 am - 10.30 am, Colin Ophus (Berkeley) Computational Imaging, data analysis, and machine learning in STEM and TEM 10.30 am - 11.00 am, Shirley Ho (Flatiron Institute) Discovering symbolic equations using deep learning 11.00 am - 11.30 am, Andrew G. Wilson, (NYU) Learning Symmetries in Neural Networks 11.30 am - 11.45 am Contributed talk 1 11.45 am - 12.00 am Contributed talks 2 1200 pm - 12.30 pm Invited talk
1.30 pm - 4.00 pm Tutorial 2: Autoencoders * Autoencoders for data cleaning * Im2spec encoder-decoder networks * Variational and invariant autoencoders
December 9 10.00 am - 10.30 am Vipin Kumar (UMN), Physics-Guided Machine Learning: A New Framework for Accelerating Scientific Discovery 10.30 am - 11.00 am Magnus Nord (NTNU) Processing very large datasets using pyXem: STEM-DPC of ferromagnetic domains 11.00 am - 11.30 am, Gerd Duscher (UTK) How can STEM be quantitative? 11.30 am - 11.45 am Contributed talk 1 11.45 am - 12.00 am Contributed talks 2 1200 pm - 12.30 pm Invited talk
December 10 10.00 am - 10.30 am Robert Howden, Maximum resolution from the Ronchigram: human vs. deep learning 10.30 am - 11.00 am Martin Jankowiak (Broad Institute) Bayesian methods for adaptive experimental design 11.00 am - 11.30 am, Dhiresha Kudithipudi (UTSA), Task Agnostic Lifelong Learning 11.30 am - 11.45 am Contributed talk 1 11.45 am - 12.00 am Contributed talk 2 12.00 pm - 12.30 pm Viren Jain (Google) Synapse-resolution connectomics in fly, bird, and human brains
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/ --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both nasadi-at-ece.ufl.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: nasadi-at-ece.ufl.edu Name: Navid Asadi
Title-Subject: [Filtered] IEEE PAINE 2020-Call for Participation
Message: Dear Colleagues,
IEEE PAINE registration is open now.
Please join if interested to hear from our outstanding speakers, or see demos from sponsors. PAINE is focused on "physical assurance and inspection of electronics" which covers, Microscopy, AI, trust and assurance, hardware security, failure analysis, etc. Program: http://paine-conference.org/program-2020/ Speakers: http://paine-conference.org/speakers-paine-2020/ Sponsors: http://paine-conference.org/sponsorship/
Login Host: 99.20.116.214 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
This year the annual Cryo Microscopy Group meeting will take place virtually on two consecutive afternoons.
On Wednesday 18th November, the talks are mostly TEM related with expert talks on Cryo Electron Tomography, MicroED and the exciting new technique of Cryo Ptychography.
On Thursday 19th November, we have talks on experimentation with cooled micromanipulators in SEM , CryoSEM in food science and developments at B24 Beamline at Diamond in Cryo X-ray and Cryo Super Resolution Microscopy
There are also going to be opportunities to engage with the trade representatives using break-out rooms to try to simulate the live meeting experience.
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O-rings are generic supply and you would be just fine with Viton material for TEM holder.
To find correct size - first measure diameter of the O-ring thread and then find closest size in a standard chart, AS568 or metric. Charts are widely available online, and if you have difficulty finding exact match then order couple of close sizes - the cost is literally pennies so just toss away the wrong size and use what fits.
There are *hundreds* places to order viton O-rings online, from generics like RubberStore where I get large quantities for pennies, to Duniway Stockroom where rubber gaskets and O-rings are cleaned, degassed in vacuum oven, and packaged for immediate use.
A word of caution - if you buy o-rings from a general rubbers supply outlet then keep in mind that they likely have been touched by hands, didn't go through critical cleaning, and weren't packaged in a cleanroom environment. They also may have seams along the edge from manufacturing. Thus you have to inspect and clean them, but difference in price is absolutely worth it.
Best Wishes, Valery
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 www.partbeamsystech.com www.fibsemproducts.com www.freudlabs.com
"Only the Paranoid Survive" (A.Grove & SpaceX QA)
On 11/13/2020 7:36 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } } X-from: Frank Karl {frank_karl-at-ardl.com} } Hello Everyone, I have just used my last 0-ring on the sample rod for our FEI CM12. We are no } longer covered by their service contract and I need to find a replacement source. I thought I had a } life time supply, but it seems I'm wrong. } I believe the O-ring is Viton but their 11 digit number doesn't mean anything outside of the FEI } walls. Does anyone have any information on size, perhaps a catalog number for a company from which } I can order from? Thanks!!! } } } } } Stay calm...Be brave....watch for signs } } Frank Karl } Microscopist } Akron Rubber Development Laboratory } 2887 Gilchrist Road } Akron, Ohio 44305 } } } ==============================Original Headers============================== } 8, 53 -- From microscopy.listserver-at-gmail.com Fri Nov 13 06:36:08 2020 } 8, 53 -- Received: from mail-il1-f175.google.com (mail-il1-f175.google.com [209.85.166.175]) } 8, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0ADCa8Fd020503 } 8, 53 -- for {microscopy-at-microscopy.com} ; Fri, 13 Nov 2020 06:36:08 -0600 } 8, 53 -- Received: by mail-il1-f175.google.com with SMTP id y17so8370891ilg.4 } 8, 53 -- for {microscopy-at-microscopy.com} ; Fri, 13 Nov 2020 04:48:53 -0800 (PST) } 8, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=gmail.com; s=20161025; } 8, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 8, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 8, 53 -- bh=NCmodS2snHRwbrdyjUXf/+vZ2isc9tfVNCt19ecNVEQ=; } 8, 53 -- b=m+KwI7QlgJMFBjz5zp2BE7WdGcoSfwI5gNEJ/YiBHM1c/n5/Ouj0AKgfZMezjtX2oq } 8, 53 -- sXyo13DLVhTF1FT5sQa/LPoxaZmcEsmYfjXuryGAPEXXdF6yZbpzUqKWc7mWlf63sBq5 } 8, 53 -- qCmqQ0Ht+kSqnvujDnfoWqbwZiqGvcnU0P4aZUfvDMppdKH12R1pOkeWLz8O96fvXjZR } 8, 53 -- gVa+IzWjyjYyxK5dSCgqLZYy2HMjCzvoWYFovmdbi5r3dq/+gRloxEXRQPpmq1URUh1/ } 8, 53 -- ud8oE82Eb6Yd6s4wuOrN6Nmy0a3fmqDFojDLV4Xjlb4eFZTGhwMOOTSuOfbFvQPggzYL } 8, 53 -- UxZQ== } 8, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 8, 53 -- d=1e100.net; s=20161025; } 8, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 8, 53 -- :user-agent:mime-version:in-reply-to:content-language } 8, 53 -- :content-transfer-encoding; } 8, 53 -- bh=NCmodS2snHRwbrdyjUXf/+vZ2isc9tfVNCt19ecNVEQ=; } 8, 53 -- b=DFzZspbr6+Bu2x6YjFEqHWQPFKDfeCArtkkSFIGPt4KyaJEsPc+3QS+owL583aVAqd } 8, 53 -- xSU3reht/q3FcnUB/WJs6wDqg+QfEckTmy0yVBmKYg7lt6J0rwia3my1SsD2bvdDcGza } 8, 53 -- AHpiw4yxTGw9zLtp6VhYrI1AHL1Yv6+rsZqQDsuFAk+6qjJftepXy27gNXQPWa2V8FeP } 8, 53 -- ksEckEvnAl9SVGJCdbZKFWaaSHCSvp+1biNboH85mL5xuDU3bzUPw8otnrBqEU2XsxVH } 8, 53 -- 8vPy1uEzPoITP2WCFYDk3weRMg4TYKi5KpAGPNWFT83/GYKlYzHuKwcD9kUnISDXDN2s } 8, 53 -- 8q/A== } 8, 53 -- X-Gm-Message-State: AOAM532TyICMtwbvLAyNvcUENeFM+WwqItkmIpIQ+QCHw9zSehLdqUv4 } 8, 53 -- Zb1wvdDzMSbAqvj148AhT+f3CItB7To= } 8, 53 -- X-Google-Smtp-Source: ABdhPJx1k8WPmaCX5KG9KtQcIXS9uSfbQjsnuk0oYrZvtxjxXN7gIMtZ1VZYOUXMyh6jZkU/1dn9YQ== } 8, 53 -- X-Received: by 2002:a92:7914:: with SMTP id u20mr1761240ilc.203.1605271733238; } 8, 53 -- Fri, 13 Nov 2020 04:48:53 -0800 (PST) } 8, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:15c5:50ab:c81:6c18]) } 8, 53 -- by smtp.googlemail.com with ESMTPSA id r3sm4421080iot.21.2020.11.13.04.48.52 } 8, 53 -- for {microscopy-at-microscopy.com} } 8, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 8, 53 -- Fri, 13 Nov 2020 04:48:52 -0800 (PST) } 8, 53 -- Subject: Fwd: O-rings } 8, 53 -- References: {BN7PR15MB2260A3B5FE9F8EEACD064C2995E80-at-BN7PR15MB2260.namprd15.prod.outlook.com} } 8, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 8, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 8, 53 -- X-Forwarded-Message-Id: {BN7PR15MB2260A3B5FE9F8EEACD064C2995E80-at-BN7PR15MB2260.namprd15.prod.outlook.com} } 8, 53 -- Message-ID: {6740aded-1eb4-4499-ab85-f0a3c7f6f987-at-gmail.com} } 8, 53 -- Date: Fri, 13 Nov 2020 06:48:51 -0600 } 8, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:78.0) } 8, 53 -- Gecko/20100101 Thunderbird/78.4.1 } 8, 53 -- MIME-Version: 1.0 } 8, 53 -- In-Reply-To: {BN7PR15MB2260A3B5FE9F8EEACD064C2995E80-at-BN7PR15MB2260.namprd15.prod.outlook.com} } 8, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 8, 53 -- Content-Language: en-US } 8, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers============================== }
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Email: tzega-at-lpl.arizona.edu Name: Tom Zega
Title-Subject: [Filtered] Researcher/Scientist III Position Opening, University of Arizona
Message: Dear Colleagues,
I would like to draw your attention to an open position for a staff scientist position in The Kuiper Materials Imaging and Characterization Facility.
} From the job posting:
The Kuiper Materials Imaging and Characterization Facility (KMICF) at the Lunar and Planetary Laboratory, University of Arizona (https://kmicf.lpl.arizona.edu) invites applications for the position of Research Scientist III. KMICF is an analytical facility dedicated to research excellence in imaging, spectroscopy, and analysis of heterogeneous materials from the millimeter to atomic scale. Laboratories include electron microprobe, scanning electron microscopy, transmission electron microscopy, and focused ion beam scanning electron microscopy. The staff scientist will work with the instrument scientist and be responsible for managing daily operations of the focused-ion-beam and scanning electron microscope laboratories.
The Research Scientist will have access to world-class electron- and ion-beam instrumentation and the opportunity to work in a dynamic research environment. KMICF will figure prominently in the analysis of samples to be returned from asteroid Bennu by NASAs OSIRIS-REx Mission (https://www.asteroidmission.org), and so the successful candidate will have an opportunity to participate in measurements of the returned sample.
The successful candidate will be a highly organized individual with knowledge of electron and ion-beam instrumentation and laboratory practices. A professional demeanor and demonstrated interpersonal skills are required for working with all levels of users including: students, postdoctoral fellows, faculty, other staff scientists, their guests and visitors, as well as the general public. Outstanding UA benefits include health, dental, and vision insurance plans; life insurance and disability programs; paid vacation, sick leave, and holidays; UA/ASU/NAU tuition reduction for the employee and qualified family members; state retirement plan; access to UA recreation and cultural activities; and more.
The University of Arizona has been listed by Forbes as one of Americas Best Employers in the United States and WorldatWork and the Arizona Department of Health Services have recognized us for our innovative work-life programs. For more information about working at the University of Arizona, please click here.
For more information on the position including how to apply, please visit https://arizona.csod.com/ux/ats/careersite/4/home/requisition/3156?c=arizona. The position will remain open until filled.
Thank you.
Tom Zega
----------------------------------------------------------------------------------------------------- Thomas J. Zega Associate Professor Lunar and Planetary Laboratory Materials Science and Engineering Scientific Director, Kuiper Materials Imaging and Characterization Facility University of Arizona 1629 E. University Blvd. Tucson AZ 85721-0092 (v) 520-626-1356 (f) 520-621-4933 https://www.lpl.arizona.edu/PMRG/ https://kmicf.lpl.arizona.edu -----------------------------------------------------------------------------------------------------
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Email: anpenn-at-ncsu.edu Name: Aubrey Penn
Title-Subject: [Filtered] Ask A Microscopist Webinar Message: Dear Microscopist, The Microscopy Society of America Student Council will be hosting the "Ask a Microscopist" webinar on November 19 for students and new microscopists to bring their practical microscopy questions to the experts. Our invited panelists span a variety of careers with both biological and physical science backgrounds, as well as light and electron microscopy expertise. Panelists will answer your questions about sample preparation, microscopy experimentation, and data analysis. Participants can register (https://forms.gle/DQZEYyxb85yDNJyU9) to receive reminders and submit questions in advance of the webinar, and join via Zoom on Thursday, November 19 at 12:00 PM ET. Participants will need to register to join us on Zoom and have the opportunity to ask questions during the panel discussion. The webinar will also be live streamed on our Facebook and Youtube pages.
StC is excited to announce that any MSA member who attends all webinars in this years Webinar Series will be entered in a drawing to win a StC merchandise package including a T-shirt, mug and more!
Our panelists are: Paul Voyles, PhD Professor of Materials Science and Engineering University of Wisconsin, Madison
Mariena Silvestry Ramos, PhD Electron Microscopy Research Support Staff Cornell Center for Materials Research
Sai Veeraraghavan, PhD Assistant Professor of Biomedical Engineering Ohio State University
Any questions can be directed to studentcouncil-at-microscopy.org. Sincerely, Aubrey Penn President-elect, MSA StC
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Email: ravi.thakkar369-at-gmail.com Name: Ravi Title-Subject: [Filtered] how to crop image in Gatan Digital Micrograph?
Message: Hi, Can anyone please help me to crop the TEM images on Gatan Digital Micrograph?
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Dear Colleagues, I am leaving my job in beautiful Switzerland and I would like to draw your attention to the job posting for my replacement as a lab manager for a small lab with a CryoSEM (Gemini500 SEM w low vac, low-kV ion gun for polishing cryo samples, EDX, STEM) , a table-top SEM and a cryoSEM prep suite from Leica (UC7, ACE600, VCT500 and loading station). The lab is integrated in the Center for Advanced Surface Analysis, which is a platform shared between EPFL and UNIL in Lausanne, Switzerland. There are several large and excellent EM facilities to collaborate with in close vicinity and plenty of possibilities for technical development. The position is permanent. https://recruiting.epfl.ch/Vacancies/1571/Description/2?fbclid=IwAR1RobCCQvND-CNbEAGzDlElK-JHF640LKM1Pwa3eHQp_V13vaS4UZurIyA Best wishes, Louise.
My lab an available postdoc position in the field of atomic-resolution cryogenic scanning transmission electron microscopy (cryo-STEM) of quantum materials. The newly formed lab has unique in situ capabilities at the interdisciplinary Rowland Institute at Harvard (https://www2.rowland.harvard.edu/) and offers exciting and collaborative research projects that aim to simultaneously image and manipulate materials with exotic quantum ground states.
The candidate will benefit from close contact with the PI at the interdisciplinary Rowland Institute and engagement with the broad quantum research community at Harvard University. Our lab has a dedicated in situ cryogenic/electrical TEM holder for custom low temperature experiments, a 1.5K/9T cryostat system for ex situ characterization of imaged devices, and a suite of sample preparation and characterization tools. For microscopy experiments, we use the aberration-corrected electron microscopes (JEOL ARM 200F, forthcoming Hitachi HF-3300), focused ion beams and other imaging tools located at the Harvard Center for Nanoscale Systems (https://cns1.rc.fas.harvard.edu/).
More details on the position and the application process are available at https://www.elbaggarilab.com/opportunities/. Pre-submission inquiries via email are welcome.
Regards,
Ismail El Baggari PhD Principal Investigator & Rowland Fellow The Rowland Institute at Harvard Cambridge, MA www.elbaggarilab.com
==============================Original Headers============================== 6, 22 -- From ielbaggari+5faeb4f6-at-rowland.harvard.edu Thu Nov 19 09:38:48 2020 6, 22 -- Received: from netrider.rowland.org (netrider.rowland.org [192.131.102.5]) 6, 22 -- by microscopy.com (8.12.11.20060308/8.12.8) with SMTP id 0AJFcmg7014763 6, 22 -- for {Microscopy-at-Microscopy.Com} ; Thu, 19 Nov 2020 09:38:48 -0600 6, 22 -- Received: (qmail 574017 invoked by uid 982); 19 Nov 2020 10:51:52 -0500 6, 22 -- Received: from 24.61.247.87 (ielbaggari-at-24.61.247.87) by netrider.rowland.org (envelope-from {ielbaggari-at-rowland.harvard.edu} , uid 982) with qmail-scanner-2.01 6, 22 -- (clamdscan: 0.102.2/25716. 6, 22 -- Clear:RC:1(24.61.247.87):. 6, 22 -- Processed in 0.031161 secs); 19 Nov 2020 15:51:52 -0000 6, 22 -- Received: from c-24-61-247-87.hsd1.ma.comcast.net (HELO ?192.168.0.23?) (ielbaggari-at-24.61.247.87) 6, 22 -- by netrider.public.rowland.org with ESMTPA; 19 Nov 2020 10:51:52 -0500 6, 22 -- From: Ismail El Baggari {ielbaggari-at-rowland.harvard.edu} 6, 22 -- Content-Type: text/plain; 6, 22 -- charset=us-ascii 6, 22 -- Mime-Version: 1.0 (Mac OS X Mail 11.5 \(3445.9.1\)) 6, 22 -- Subject: Post-doc: cryogenic STEM of quantum materials 6, 22 -- Message-Id: {90FB673C-A0CD-4C89-B59C-759BFB6C2CB1-at-rowland.harvard.edu} 6, 22 -- Date: Thu, 19 Nov 2020 10:51:51 -0500 6, 22 -- To: Microscopy-at-Microscopy.Com 6, 22 -- X-Mailer: Apple Mail (2.3445.9.1) 6, 22 -- Content-Transfer-Encoding: 8bit 6, 22 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 0AJFcmg7014763 ==============================End of - Headers==============================
I am looking for an antibody to be used as a peroxisomal marker at the TEM level on sections of resin-embedded plant tissue, preferably a rabbit polyclonal, but a monoclonal would also work.
Any recommendations, also on working dilutions, would be very much appreciated!
Thanks a lot in advance, Ulla Neumann
Central Microscopy, MPI Plant Breeding Research, Cologne, Germany
==============================Original Headers============================== 5, 26 -- From neumann-at-mpipz.mpg.de Fri Nov 20 03:28:41 2020 5, 26 -- Received: from mr.service.mpipz.mpg.de (mr.service.mpipz.mpg.de [195.37.46.46]) 5, 26 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0AK9Se1H017770 5, 26 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2020 03:28:41 -0600 5, 26 -- Received: from mta02.mpipz.mpg.de ([10.1.0.104]) 5, 26 -- by mr.service.mpipz.mpg.de with ESMTP id 0AK9fkoN010149-0AK9fkoP010149 5, 26 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NO) 5, 26 -- for {Microscopy-at-microscopy.com} ; Fri, 20 Nov 2020 10:41:47 +0100 5, 26 -- Received: from [10.10.18.1] (helo=[10.1.19.21]) 5, 26 -- by mta02.mpipz.mpg.de with esmtpsa (TLS1.3:ECDHE_RSA_AES_128_GCM_SHA256:128) 5, 26 -- (Exim 4.92) 5, 26 -- (envelope-from {neumann-at-mpipz.mpg.de} ) 5, 26 -- id 1kg2vK-0003mU-UF 5, 26 -- for Microscopy-at-microscopy.com; Fri, 20 Nov 2020 10:41:46 +0100 5, 26 -- To: Microscopy-at-microscopy.com 5, 26 -- From: Ulla Neumann {neumann-at-mpipz.mpg.de} 5, 26 -- Subject: TEM: plant peroxisomal marker for IGL 5, 26 -- Message-ID: {7a50be7c-95c0-1dd4-c6bd-f0396734ed8b-at-mpipz.mpg.de} 5, 26 -- Date: Fri, 20 Nov 2020 10:41:46 +0100 5, 26 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; Win64; x64; rv:78.0) Gecko/20100101 5, 26 -- Thunderbird/78.4.0 5, 26 -- MIME-Version: 1.0 5, 26 -- Content-Type: text/plain; charset=utf-8; format=flowed 5, 26 -- Content-Transfer-Encoding: 7bit 5, 26 -- Content-Language: en-US 5, 26 -- X-FEAS-SYSTEM-WL: 10.1.0.104 ==============================End of - Headers==============================
Could someone remind me when and by which manufacturer (on which instrument) the first in lens SE detector was implemented on a SEM ? And if you could point me on some papers ?
I know that in the deveoppement of the STEM by Akashi et al. on the JEOL 100 CX TEM (~1970 ?) they put a SE detector above the upper polepiece (no room enough between the two polepieces) and got a "pure" SE signal. But I don't remember when it was for the SEM and if Jeol did that. Or was it ISI/ABT ?
Many thanks and best regards
Jacques
--
J. Faerber IPCMS-DSI Institut de Physique et Chimie des Matériaux de Strasbourg Département Surfaces et Interfaces 23, rue de Loess ; BP43 67034 Strasbourg CEDEX 2 France
E-mail : Jacques.Faerber-at-ipcms.unistra.fr
==============================Original Headers============================== 9, 21 -- From jacques.faerber-at-ipcms.unistra.fr Fri Nov 20 15:34:27 2020 9, 21 -- Received: from webmail.ipcms.fr (webmail.ipcms.fr [130.79.210.2]) 9, 21 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0AKLYQkI006612 9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2020 15:34:27 -0600 9, 21 -- Received: from [192.168.1.19] (lfbn-str-1-583-186.w90-126.abo.wanadoo.fr [90.126.47.186]) 9, 21 -- (Authenticated sender: jacques.faerber-at-ipcms.unistra.fr) 9, 21 -- by webmail.ipcms.fr (Postfix) with ESMTPSA id 5EA4F406E5 9, 21 -- for {microscopy-at-microscopy.com} ; Fri, 20 Nov 2020 22:47:34 +0100 (CET) 9, 21 -- From: jfaerber {jacques.faerber-at-ipcms.unistra.fr} 9, 21 -- Subject: SEM first in lens SE detector ? 9, 21 -- To: microscopy-at-microscopy.com 9, 21 -- Message-ID: {c841001a-b1dd-d4a1-32d9-34fc5150ec54-at-ipcms.unistra.fr} 9, 21 -- Date: Fri, 20 Nov 2020 22:47:33 +0100 9, 21 -- User-Agent: Mozilla/5.0 (X11; Linux x86_64; rv:78.0) Gecko/20100101 9, 21 -- Thunderbird/78.4.0 9, 21 -- MIME-Version: 1.0 9, 21 -- Content-Type: text/plain; charset=utf-8; format=flowed 9, 21 -- Content-Transfer-Encoding: 8bit 9, 21 -- Content-Language: fr 9, 21 -- X-Bm-Milter-Handled: f5e5d9bb-a6db-4872-b8bc-cffd451f4758 9, 21 -- X-Bm-Transport-Timestamp: 1605908854458 ==============================End of - Headers==============================
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Message: We recently had to shut down our JEOL 2010 for a repair. When we restarted the TEM, we found the JEOL built-in computer had some issues. The computer had a program called "HTUP" which allowed the user to program the HT to move up from one kV to another, over a prescribed time period and in prescribed kV steps. Now the computer says "file not found" when we try to use that program. Does anyone have such a program on their 2010 that they can share? Otherwise I'll have to figure out the JEOL rudimentary programming language and re-create the program. Not looking forward to that!
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Email: desertrat99-at-verizon.net Name: Eric Rosen
Title-Subject: [Filtered] Fixing a EM208S system. Message: Does anyone know someone who can service a EM208S? I am having a problem with the High Tension. The system will all me to scope for a while. When unused we leave the vacuum system on all the time. Then all of the sudden the HT will not energize when I push and hold the button. To get it working again I have to shutdown the entire system and wait overnight. Power up the scope and vacuum and the HT work again for a while. Any suggestions? I have not smelled anything burning or melting plastic on the circuit boards. I do not have enough skill set to test out the HT rack boards. Login Host: 149.142.103.136 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
I once had an EM208 my own but sold it some time later. Unfortunately I don`t have any manuals left to help you or somebody electronically knowledgable to the exact point where to look...
...
Some questions:
- Did the HT voltage switch off on its own after some time when working with the scope? That would point more to a heat problem in the elctronics... Or a faulty relay or missing 24 volts control voltage (but then the whole scope will shut down...)
- "HT voltage ON" state depends on a lot of closed "interlock" states from various (safety) circuit in the instrument, like sufficient air pressure, cooling water, temperature of power regulators, vacuum pressure etc.
There might be something wrong or coming up wrong when using the scope for some time. Voltage at one of the power supplies might just be right when electronics is cold and the be out of spec when warm...
To find out you have to provide more information how exactly and when the error occurs.
...
Did you change the high tension voltage at some point and does the problem also arise ? Lower HT voltage and lower magnification mostly means lower power needed from the supplies and maybe - if this is a thermally induced behaviour - the drop-out will come later. You have to check this first...
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 22.11.20 um 02:08 schrieb microscopy.listserver-at-gmail.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: desertrat99-at-verizon.net } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://www.microscopy.com/MLFormMail.html } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } desertrat99-at-verizon.net, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers } can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: desertrat99-at-verizon.net Name: Eric Rosen } } Title-Subject: [Filtered] Fixing a EM208S system. } Message: Does anyone know someone who can service a EM208S? I am having a problem with the High } Tension. } The system will all me to scope for a while. When unused we leave the vacuum system on all the } time. Then all of the sudden the HT will not energize when I push and hold the button. } To get it working again I have to shutdown the entire system and wait overnight. Power up the } scope and vacuum and the HT work again for a while. } Any suggestions? } I have not smelled anything burning or melting plastic on the circuit boards. I do not have enough } skill set to test out the HT rack boards. } Login Host: 149.142.103.136 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 5, 53 -- From microscopy.listserver-at-gmail.com Sat Nov 21 19:01:11 2020 } 5, 53 -- Received: from mail-il1-f182.google.com (mail-il1-f182.google.com [209.85.166.182]) } 5, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0AM11BBh022234 } 5, 53 -- for {microscopy-at-microscopy.com} ; Sat, 21 Nov 2020 19:01:11 -0600 } 5, 53 -- Received: by mail-il1-f182.google.com with SMTP id w10so12136601ilq.5 } 5, 53 -- for {microscopy-at-microscopy.com} ; Sat, 21 Nov 2020 17:14:24 -0800 (PST) } 5, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 5, 53 -- d=gmail.com; s=20161025; } 5, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 5, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 5, 53 -- bh=OUKO+a378WkAABPj5VJN1fchZ2TWUKILBga1V2OY5Po=; } 5, 53 -- b=RrruQAdtVT7AoLUBDV8qbfmEZz2giRHM3rq+QF2g0Y5GEybhvOFLcZOOst5nFsgz4A } 5, 53 -- q+IZPv9J4UIOreU2FXNb9Y8pN0t3RvGApQOYCxRlHeLeSRoBo4vIF6xGbomsKzFd/92J } 5, 53 -- sqfNtdm3gZ7hJYZpOq2YPEI/BytVwsqFM1xcrlQa4+/rfMJKf3916j87YkjQ04OhAuwM } 5, 53 -- YJ9B7Npa7wVrtkkX4zFJ1fO86Isll9QM59XbnyW9GMAQv9f9DNg4/6I8CNCu7YsIZVV1 } 5, 53 -- VgjOZAN0hQ0CojofSApiqRH3A+9jZI6NkooXG59uPZcP8fxY2ykTy4qe4uBZ40LMe0nq } 5, 53 -- v0Tg== } 5, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 5, 53 -- d=1e100.net; s=20161025; } 5, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 5, 53 -- :user-agent:mime-version:in-reply-to:content-language } 5, 53 -- :content-transfer-encoding; } 5, 53 -- bh=OUKO+a378WkAABPj5VJN1fchZ2TWUKILBga1V2OY5Po=; } 5, 53 -- b=MmqM95w5az49EQbq5gxGUyEwJ/sTlir4XDL8H4JHV880Q5HFydvNEOWI3RSFLSPum8 } 5, 53 -- w6k3IC6QhH8C5KX6JyKe57dm+7YRgpm+wCJRd6me/h7FGjfN/aB86FiL2bU/sc/Vw3gy } 5, 53 -- kRZjg1DlwH6YsecgzxHg2lUDAgz5/9Gf4BtsGkR0XJMEUuXnc4xyAhBu0fvofsTILETc } 5, 53 -- 7IpaGbUS+GrusZCILM2dwofL7zLX6Vowi2BvcBqW8eR4hiuWHZgDFCSEyYomgEJ8V2ZE } 5, 53 -- HSA2wOmZkmJzmTyj8XCrfxsytH46jltq1jWp7L1yuqg8CYS0Bx6Jeo7kI69Kjm9pcvFy } 5, 53 -- mvgw== } 5, 53 -- X-Gm-Message-State: AOAM531beeXdoUpLY68+f9mmcx2+EdIBDCkMlx8aZfZM07QoVTWchWS/ } 5, 53 -- 1P7BHZlLdlzvlPv9on+ouIdcVlq+cJE= } 5, 53 -- X-Google-Smtp-Source: ABdhPJwVfhBN8phkerMg5wmzZVYx4l02Vq/JzmHQm8vlNzbXfW9iymMAXGg28zpytyJSyJIi+ulmPg== } 5, 53 -- X-Received: by 2002:a92:7109:: with SMTP id m9mr32112815ilc.232.1606007663800; } 5, 53 -- Sat, 21 Nov 2020 17:14:23 -0800 (PST) } 5, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:c526:6806:f172:ccbc]) } 5, 53 -- by smtp.googlemail.com with ESMTPSA id u1sm4619919ilb.74.2020.11.21.17.14.23 } 5, 53 -- for {microscopy-at-microscopy.com} } 5, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 5, 53 -- Sat, 21 Nov 2020 17:14:23 -0800 (PST) } 5, 53 -- Subject: viaWWW:Fixing a EM208S system } 5, 53 -- References: {202011201820.0AKIKiwA027099-at-microscopy.com} } 5, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 5, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 5, 53 -- X-Forwarded-Message-Id: {202011201820.0AKIKiwA027099-at-microscopy.com} } 5, 53 -- Message-ID: {ee7dd8ae-bd49-3294-cf2c-20ef5655e756-at-gmail.com} } 5, 53 -- Date: Sat, 21 Nov 2020 19:14:22 -0600 } 5, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:78.0) } 5, 53 -- Gecko/20100101 Thunderbird/78.5.0 } 5, 53 -- MIME-Version: 1.0 } 5, 53 -- In-Reply-To: {202011201820.0AKIKiwA027099-at-microscopy.com} } 5, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 5, 53 -- Content-Language: en-US } 5, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
==============================Original Headers============================== 19, 30 -- From diller-at-stefan-diller.com Sun Nov 22 03:33:10 2020 19, 30 -- Received: from mailout018.rox.net (mailout018.rox.net [212.63.85.218]) 19, 30 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0AM9X9pi001904 19, 30 -- for {microscopy-at-microscopy.com} ; Sun, 22 Nov 2020 03:33:10 -0600 19, 30 -- Received: from localhost ([127.0.0.1]) 19, 30 -- by mailout01.rox.net with esmtp (Exim 4.80) 19, 30 -- (envelope-from {diller-at-stefan-diller.com} ) 19, 30 -- id 1kglwt-0002A6-DX; Sun, 22 Nov 2020 10:46:23 +0100 19, 30 -- Received: from ip1f10fd5c.dynamic.kabel-deutschland.de ([31.16.253.92] helo=mac-pro.local) 19, 30 -- by mailout01.rox.net with esmtpsa (TLSv1.2:AES128-GCM-SHA256:128) 19, 30 -- (Exim 4.80) 19, 30 -- (envelope-from {diller-at-stefan-diller.com} ) 19, 30 -- id 1kglwt-00029z-8t; Sun, 22 Nov 2020 10:46:23 +0100 19, 30 -- Subject: Re: [Microscopy] viaWWW:Fixing a EM208S system 19, 30 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 19, 30 -- desertrat99-at-verizon.net 19, 30 -- References: {202011220108.0AM18Zm9002688-at-microscopy.com} 19, 30 -- From: stefan diller {diller-at-stefan-diller.com} 19, 30 -- Message-ID: {1633f823-fd91-9156-85fa-971678d10439-at-stefan-diller.com} 19, 30 -- Date: Sun, 22 Nov 2020 10:46:22 +0100 19, 30 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:78.0) 19, 30 -- Gecko/20100101 Thunderbird/78.5.0 19, 30 -- MIME-Version: 1.0 19, 30 -- In-Reply-To: {202011220108.0AM18Zm9002688-at-microscopy.com} 19, 30 -- Content-Type: text/plain; charset=windows-1252; format=flowed 19, 30 -- Content-Language: de-DE 19, 30 -- X-Envelope-From: {diller-at-stefan-diller.com} 19, 30 -- X-Scanned-By: rockenstein AG 19, 30 -- Content-Transfer-Encoding: 8bit 19, 30 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 0AM9X9pi001904 ==============================End of - Headers==============================
The Materials Characterization Core at Drexel University is hiring a Research Instrument Specialist to manage our scanning electron microscopes (2 SEMs and a FIBSEM). Complete information and a link to apply can be found on Drexel's Careers site:
The Materials Characterization Core (MCC) is a centrally managed shared-instrumentation facility that provides Drexel University and external users access to advanced materials characterization instrumentation, training and expertise. The facility is centrally administered through the University's Office of Research and Innovation and has been providing research instrumentation access for over 14 years. The Research Instrumentation Specialist reports to the facility's full-time Director of Operations.
Essential Functions of the Research Instrumentation Specialist
* Provide outstanding service to MCC users through comprehensive training MCC SEMs, FIBSEM and sample preparation equipment. Applicant should be able to train a user who has no prior knowledge of the instruments * Assist trained users to optimize the instrument and samples for their desired experiment * Coordinate the maintenance of the instruments * Provide expert consultation on experimental design and applications * Manage users and track & confirm usage of instruments using iLab software * Develop multimedia training materials for users * Advise Director of Operations and others on instrument acquisitions and upgrades * Other duties as assigned
Thanks, Craig Johnson, Ph.D. Director of Operations TEM & FIBSEM Manager Materials Characterization Core (MCC) drexel.edu/core-facilities/facilities/material-characterization/
I'm trying to fix one old Leica AFS (automatic freeze substitution) unit now.
Does anyone have the electric scheme for this old but still good machine?
Thank you in advance, Denis *--------------------------- * *Denis Korneev, PhD * Research Fellow School of Biological Sciences Monash University 25 Rainforest Walk, 3800 Clayton (Australia)
Ravi, Select the region you wish to keep using the rectangular ROI tool (for GMS3 right-click on the image to select the tool or for regular raster images this is the default tool so hold ctrl-key left click and drag to select the ROI). Copy the region to the clip board (ctrl-c) and paste to a new image (ctrl-alt-v) to create the cropped version. The "paste new image" is a one of those legacy features that does not show up in the menus (I know it really should). There is a list of these features in the help file which I have copied below.
Best regards, Ray
Shortcuts DigitalMicrograph provides many keyboard shortcuts. Most of those shortcuts are equivalent to menu items and the shortcut can be read on the menu item. For example in the File menu, the Open... item has "Ctrl+O" listed on it. This means that pressing the "O" key, while holding down the Control key will be equivalent to choosing Open... from the File menu. Some other shortcuts in DigitalMicrograph are not displayed on menu items: Shift-Ctrl-W Close all windows, but ask the user whether to save them, if needed. Alt-Shift-Ctrl-W Close all windows, including the Results window, and do not ask the user if data needs to be saved. If you want to just close all the image windows, you can hold down the Alt, Ctrl and Shift keys and then click in the close box of one of the image windows. In a similar way can you close all script windows. Alt-Ctrl-W Close the foremost window without asking the user if the related data needs to be saved. You can also hold down the Alt key, and then click in the close box of the image window you want to close. Alt-Ctrl-F4 Close DigitalMicrograph without asking the user to save any data. Alt-Ctrl-V Paste image data in the paste buffer in a new image document window. Alt-Ctrl-X Cut the ROI from the image. With the normal Cut command on the File menu, or with Ctrl-X, you cut the data in the ROI. When you hold down the Alt key as well, you actually cut the ROI itself, which you can then paste into another document. Alt-Ctrl-C Copy the ROI from the image. With the normal Copy command on the File menu, or with Ctrl-C, you copy the data in the ROI. When you hold down the Alt key as well, you actually copy the ROI itself, which you can then paste into another document. Alt-Ctrl-H Show the options dialog for setting the number of channels in the Histogram and then display the histogram. See "Using Histograms". Alt-Ctrl-T Show the options dialog for setting the number of channels in the Histogram and then display the histogram for Thresholding. See "Thresholding".
Ray D. Twesten, Ph.D. Product Manager - Analytical Instruments Gatan, Inc. Tel. +1 (925) 224-7392
This message and any attachments are solely for the use of intended recipients. They may contain privileged and/or confidential information.
-----Original Message----- X-from: microscopy.listserver-at-gmail.com {microscopy.listserver-at-gmail.com} Sent: Wednesday, November 18, 2020 2:55 PM To: Ray Twesten {Ray.Twesten-at-ametek.com}
X-from: Tom Schamp {tom.schamp-at-protonmail.com}
Hi Ravi,
I think the easiest way is through scripting (ctrl + k). So if you are trying to crop image A, the command to create a new image that is a cropped version of A where you have already defined a rectangular ROI is "AA=A[]"
Note that you will not be transferring any tags or attributes to the new image.
Best regards,
Tom
Sent with ProtonMail Secure Email.
‐‐‐‐‐‐‐ Original Message ‐‐‐‐‐‐‐ On Wednesday, November 18, 2020 5:01 PM, {microscopy.listserver-at-gmail.com} wrote:
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as my last email asking for anti-peroxisomal antibodies to be used for IGL of resin-embedded plant tissues at the TEM level yielded zero replies, I modify my request and switch to the LM level.
Can someone recommend an antibody to be used as a peroxisomal marker for IFL/IC at the LM level on sections of resin-embedded plant tissue (preferably a rabbit polyclonal, but a monoclonal would also work).
Any recommendations, also on working dilutions, would be very much appreciated!
Thanks a lot in advance, Ulla Neumann
Central Microscopy, MPI Plant Breeding Research, Cologne, Germany
==============================Original Headers============================== 9, 27 -- From neumann-at-mpipz.mpg.de Tue Nov 24 01:41:43 2020 9, 27 -- Received: from mr.service.mpipz.mpg.de (mr.service.mpipz.mpg.de [195.37.46.46]) 9, 27 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0AO7fg4Q012426 9, 27 -- for {microscopy-at-microscopy.com} ; Tue, 24 Nov 2020 01:41:43 -0600 9, 27 -- Received: from mta02.mpipz.mpg.de ([10.1.0.104]) 9, 27 -- by mr.service.mpipz.mpg.de with ESMTP id 0AO7t145017472-0AO7t147017472 9, 27 -- (version=TLSv1.2 cipher=ECDHE-RSA-AES256-SHA bits=256 verify=NO) 9, 27 -- for {Microscopy-at-microscopy.com} ; Tue, 24 Nov 2020 08:55:01 +0100 9, 27 -- Received: from [10.10.18.1] (helo=[10.1.19.21]) 9, 27 -- by mta02.mpipz.mpg.de with esmtpsa (TLS1.3:ECDHE_RSA_AES_128_GCM_SHA256:128) 9, 27 -- (Exim 4.92) 9, 27 -- (envelope-from {neumann-at-mpipz.mpg.de} ) 9, 27 -- id 1khTAD-0002yC-L7 9, 27 -- for Microscopy-at-microscopy.com; Tue, 24 Nov 2020 08:55:01 +0100 9, 27 -- To: Microscopy-at-microscopy.com 9, 27 -- From: Ulla Neumann {neumann-at-mpipz.mpg.de} 9, 27 -- Subject: LM: antibodies to label plant peroxisomes by IFL/IC 9, 27 -- Message-ID: {257c64e3-193d-3ecb-ee38-6b986acf444c-at-mpipz.mpg.de} 9, 27 -- Date: Tue, 24 Nov 2020 08:55:01 +0100 9, 27 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; Win64; x64; rv:78.0) Gecko/20100101 9, 27 -- Thunderbird/78.4.0 9, 27 -- MIME-Version: 1.0 9, 27 -- Content-Type: text/plain; charset=utf-8; format=flowed 9, 27 -- Content-Language: en-US 9, 27 -- X-FEAS-SYSTEM-WL: 10.1.0.104 9, 27 -- Content-Transfer-Encoding: 8bit 9, 27 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 0AO7fg4Q012426 ==============================End of - Headers==============================
a skin (punch) biopsy was cut in half, one half was embedded in paraffin and H&E stained, the other half was epon-embedded and methylene blue/Azur II stained. Some parts of the biopsy are made of freshly healed tissue and are therefore devoid of collagen fibers. This is very easy to see with H&E. But what about methylene blue/azur II? Is there any chance that collagen is stained as well? As a biologist I was trained to recognize healthy tissue but I have a hard tissue analyzing abnormal tissue patterns. Can a pathologist help me here?
-----Ursprngliche Nachricht----- Von: MUSS Wolfgang Dr. phil./PhD (OR i.R, retired) [mailto:wij.muss-at-aon.at] Gesendet: Donnerstag, 26. November 2020 16:26 An: 'nizets2-at-yahoo.com' {nizets2-at-yahoo.com} Cc: 'Microscopy-at-Microscopy.com' {Microscopy-at-Microscopy.com} but unfortunately as 'html'
So this time forwarded only to {Microscopy-at-Microscopy.com}
Conc.: Betreff: Re: [Microscopy] methylene blue/Azur II staining
A semithin resin section - depending on the former processing (starting with fixation - ending with resin mixture and polymerization) - of a skin punch biopsy containing collagen fibrils stained with Methylene blue / Azure II alone (depending on the execution of a specific modification or use of staining kit...so it would be not only interesting which method is used to achieve prominent staining without overstaining) not necessarily might stain ('all') collagen fibrils / fibers.
In the case of using an 'AMbF' (Azure II-Methylene blue-basic Fuchsin)-stain sequence (e.g. HUMPHREY and PITTMAN 1965, unmodified [depending on the resin used] or modified by myself in the 1980ies and routinely used until 2015 for diagnostic LM-TEM) I could nearly guarantee that you achieve positive red staining for collagen fibrils/fibersas said depending on the previous processing parameters.
Only as a notice added in brief: in the mid 1980ies there was a change in the sources of EPON 812. At that time I noticed that following the processing according to HUMPHREY and PITTMAN, as well as LACZK and LVAI resulted in no more optimal staining for unknown reason. After a certain modification (not published yet) I was able to get reasonable results again (without overstaining).
But it might be that you already have seen some semithin section stainings of this kind elsewhere [as such are displayed in Conference presentations I uploaded them in the ResearchGate Database under http://www.researchgate.net/profile/Wolfgang_MUSS ]
References: HUMPHREY Ch. D., PITTMAN F.E. (1974) (Azur- II -Methylenblau-basisches Fuchsin) A simple Methyleneblue-Azure II Basic Fuchsin Stain for Epoxy-Embedded Tissue Sections Stain Technology 49, 9-14 (1974)
or cf. also LACZK Jen and LVAI Geza A Simple Differential Staining Method for Semi-thin Sections of Ossifying Cartilage and Bone Tissues Embedded in Epoxy Resin (Eine einfache Frbemethode zur Differenzierung des verkalkenden Knorpel. und Knochengewebes nach Einbettung in Epoxy[Epoxid]harz) Mikroskopie (WIEN/Vienna), 31(1975) p. 1-4
Further information you might find in the MSA-List server archives of the 1980ies / 1990ies
Best regards, W.H.M
MUSS Wolfgang Dr. phil. (PhD) [OR i. R. / en retraite / retired]
FRMS, Retired Member of MSA, Member of BSC and other Societies
Scientific Profile at ResearchGate: http://www.researchgate.net/profile/Wolfgang_MUSS Former Head of Electron Microscopy Lab at Institute of Pathology SALK-LKH / Salzburger Landeskliniken | General Hospital and PMU (private) PARACELSUS MEDICAL UNIVERSITY of SALZBURG
-----Ursprngliche Nachricht----- Von: nizets2-at-yahoo.com [mailto:nizets2-at-yahoo.com] Gesendet: Donnerstag, 26. November 2020 12:59 An: wij.muss-at-aon.at Betreff: [Microscopy] methylene blue/Azur II staining
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Dear colleagues,
a skin (punch) biopsy was cut in half, one half was embedded in paraffin and H&E stained, the other half was epon-embedded and methylene blue/Azur II stained. Some parts of the biopsy are made of freshly healed tissue and are therefore devoid of collagen fibers. This is very easy to see with H&E. But what about methylene blue/azur II? Is there any chance that collagen is stained as well? As a biologist I was trained to recognize healthy tissue but I have a hard tissue analyzing abnormal tissue patterns. Can a pathologist help me here?
I am wondering if someone on the list may have schematics for "BB 8kV" Beam Blank Controller made by Raith? I have a unit with a peculiar failure of switching power supply board that generates 8.25kV output. Reversing the design isn't a big deal, but takes time and working with readily available schematics could be so much easier and quicker...
Thanks beforehand, Valery -- Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 www.partbeamsystech.com www.fibsemproducts.com www.freudlabs.com
collagen usually stains well in semithin sections with methylene blue/Azur II aka Richardson Blue. In some cases it can become difficult to discriminate collagen layers / fibers e.g. if tightly adjacent cells are stained heavily or e.g. you won't be able to discriminate collagen layers from myelin in myelinated nerves, or, another example, collagen layers of arteries or arterioles usually stain very well with Richardson Blue. Please contact me offline if you want to have some examplary pictures...
I am curious to learn if anyone on the list has a method to deposit a thin layer of quartz/SiO2 on a QCM (quartz balance crystal) on top of the gold or silver layer of the QCM crystal or a company that can do it?
Thanks beforehand,
Vincent Carlino ibss Group, Inc. www.ibssgroup.com 111 Anza Blvd. Suite 110 Burlingame, CA 94010 T: 650.513.1488 C: 415.900.7193
==============================Original Headers============================== 7, 32 -- From vince.carlino-at-ibssgroup.com Mon Nov 30 00:27:14 2020 7, 32 -- Received: from smtp115.ord1c.emailsrvr.com (smtp115.ord1c.emailsrvr.com [108.166.43.115]) 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0AU6RE1Z023498 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2020 00:27:14 -0600 7, 32 -- Received: from smtp192.mex09.emailsrvr.com (unknown [184.106.73.70]) 7, 32 -- by smtp7.relay.ord1c.emailsrvr.com (SMTP Server) with ESMTPS id 4C0D4A0083 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2020 01:40:54 -0500 (EST) 7, 32 -- Received: from MBX05C-ORD1.mex09.mlsrvr.com (172.29.128.24) by 7, 32 -- MBX05C-ORD1.mex09.mlsrvr.com (172.29.128.24) with Microsoft SMTP Server 7, 32 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id 7, 32 -- 15.1.1913.5; Mon, 30 Nov 2020 00:40:53 -0600 7, 32 -- Received: from MBX05C-ORD1.mex09.mlsrvr.com ([fe80::d047:39db:d71d:cdc8]) by 7, 32 -- MBX05C-ORD1.mex09.mlsrvr.com ([fe80::d047:39db:d71d:cdc8%20]) with mapi id 7, 32 -- 15.01.1913.007; Mon, 30 Nov 2020 00:40:53 -0600 7, 32 -- From: Vincent Carlino {vince.carlino-at-ibssgroup.com} 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} 7, 32 -- Subject: Deposit thin layer of quartz/SiO2 on a QCTM crystal 7, 32 -- Thread-Topic: Deposit thin layer of quartz/SiO2 on a QCTM crystal 7, 32 -- Thread-Index: AdbG47PvDzbZS0xISxe8Hsow40j2EA== 7, 32 -- Date: Mon, 30 Nov 2020 06:40:53 +0000 7, 32 -- Message-ID: {3c2581508ba045e28b6fa77659cdf748-at-ibssgroup.com} 7, 32 -- Accept-Language: en-US 7, 32 -- Content-Language: en-US 7, 32 -- X-MS-Has-Attach: 7, 32 -- X-MS-TNEF-Correlator: 7, 32 -- x-originating-ip: [69.181.198.10] 7, 32 -- Content-Type: text/plain; charset="us-ascii" 7, 32 -- MIME-Version: 1.0 7, 32 -- X-Classification-ID: 1cf2d350-48a2-4907-9110-198bfe504760-1-1 7, 32 -- X-Sender-ID: vince.carlino-at-ibssgroup.com 7, 32 -- Content-Transfer-Encoding: 8bit 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 0AU6RE1Z023498 ==============================End of - Headers==============================
Either dielectric (optical) E-Beam evaporator or RF sputter coater would deposit very thin film of SiO2. I am familiar with Denton equipment, but I'm sure there are others. Many University-based semiconductor facilities would have these tools, just Google.
Thicker layers may be easier to deposit via SOD (Spin-On Dielectric) or SOG (Spin-On Glass) formulas made for semiconductor manufacturing. I've used Honeywell, but others are without doubt are also available.
Valery Ray (also with REFINE Lab, UCONN) ========================================== MEO Engineering Company, Inc. DBA "PBS&T" 290 Broadway, Suite 298, Methuen MA 01844 E-mail: vray-at-partbeamsystech.com Phone: +1-978-305-0479 www.partbeamsystech.com www.fibsemproducts.com www.freudlabs.com
"Only the Paranoid Survive" (A.Grove & SpaceX QA)
On 11/30/2020 1:27 AM, vince.carlino-at-ibssgroup.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } I am curious to learn if anyone on the list has a method to deposit a thin layer of quartz/SiO2 on a QCM (quartz balance crystal) on top of the gold or silver layer of the QCM crystal or a company that can do it? } } Thanks beforehand, } } Vincent Carlino } ibss Group, Inc. } www.ibssgroup.com } 111 Anza Blvd. Suite 110 } Burlingame, CA 94010 } T: 650.513.1488 } C: 415.900.7193 } } } } } } ==============================Original Headers============================== } 7, 32 -- From vince.carlino-at-ibssgroup.com Mon Nov 30 00:27:14 2020 } 7, 32 -- Received: from smtp115.ord1c.emailsrvr.com (smtp115.ord1c.emailsrvr.com [108.166.43.115]) } 7, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0AU6RE1Z023498 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2020 00:27:14 -0600 } 7, 32 -- Received: from smtp192.mex09.emailsrvr.com (unknown [184.106.73.70]) } 7, 32 -- by smtp7.relay.ord1c.emailsrvr.com (SMTP Server) with ESMTPS id 4C0D4A0083 } 7, 32 -- for {Microscopy-at-microscopy.com} ; Mon, 30 Nov 2020 01:40:54 -0500 (EST) } 7, 32 -- Received: from MBX05C-ORD1.mex09.mlsrvr.com (172.29.128.24) by } 7, 32 -- MBX05C-ORD1.mex09.mlsrvr.com (172.29.128.24) with Microsoft SMTP Server } 7, 32 -- (version=TLS1_2, cipher=TLS_ECDHE_RSA_WITH_AES_128_CBC_SHA256_P256) id } 7, 32 -- 15.1.1913.5; Mon, 30 Nov 2020 00:40:53 -0600 } 7, 32 -- Received: from MBX05C-ORD1.mex09.mlsrvr.com ([fe80::d047:39db:d71d:cdc8]) by } 7, 32 -- MBX05C-ORD1.mex09.mlsrvr.com ([fe80::d047:39db:d71d:cdc8%20]) with mapi id } 7, 32 -- 15.01.1913.007; Mon, 30 Nov 2020 00:40:53 -0600 } 7, 32 -- From: Vincent Carlino {vince.carlino-at-ibssgroup.com} } 7, 32 -- To: "Microscopy-at-microscopy.com" {Microscopy-at-microscopy.com} } 7, 32 -- Subject: Deposit thin layer of quartz/SiO2 on a QCTM crystal } 7, 32 -- Thread-Topic: Deposit thin layer of quartz/SiO2 on a QCTM crystal } 7, 32 -- Thread-Index: AdbG47PvDzbZS0xISxe8Hsow40j2EA== } 7, 32 -- Date: Mon, 30 Nov 2020 06:40:53 +0000 } 7, 32 -- Message-ID: {3c2581508ba045e28b6fa77659cdf748-at-ibssgroup.com} } 7, 32 -- Accept-Language: en-US } 7, 32 -- Content-Language: en-US } 7, 32 -- X-MS-Has-Attach: } 7, 32 -- X-MS-TNEF-Correlator: } 7, 32 -- x-originating-ip: [69.181.198.10] } 7, 32 -- Content-Type: text/plain; charset="us-ascii" } 7, 32 -- MIME-Version: 1.0 } 7, 32 -- X-Classification-ID: 1cf2d350-48a2-4907-9110-198bfe504760-1-1 } 7, 32 -- X-Sender-ID: vince.carlino-at-ibssgroup.com } 7, 32 -- Content-Transfer-Encoding: 8bit } 7, 32 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 0AU6RE1Z023498 } ==============================End of - Headers============================== }
The Analytical Instrumentation Facility (AIF) at North Carolina State University (NCSU) is seeking a talented and industrious experimentalist to join our team as an X-ray Microscope (XRM) Postdoctoral Researcher. Representative duties include training users on operation of the X-ray microscope and CT instrument, performing X-ray microscopy services using Zeiss Xradia 510 and the Bruker SkyScan 1174 instruments, implementing at least 4 workshops and/or user short courses each year, and staying abreast of software and hardware developments by reading current literature and attending relevant technical conferences.
For more details and to apply, please visit: https://jobs.ncsu.edu/postings/137551
Thanks, Chris
-- Transmission Electron Microscopy Lab Manager Analytical Instrumentation Facility (AIF) NC State University https://www.aif.ncsu.edu/ Cell: 267-496-0587
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Title-Subject: [Filtered] Job opening, EM core facility at St. Jude Children's Research Hospital
Message: Hello all,
The EM Shared Resource at St. Jude Childrens Research Hospital is hiring at up to the Associate Scientist level for an electron microscopist to join their team. Full details and a link to apply can be found at: https://careers-stjude.icims.com/jobs/6112/associate-scientist/job?mode=view&mobile=false&width=1180&height=500&bga=true&needsRedirect=false&jan1offset=-360&jun1offset=-300
The Cell and Tissue Imaging Center at St. Jude Childrens Research Hospital is looking for a talented and driven electron microscopist to join the Center at the Associate Scientist level. The electron microscopy facility houses a Thermo Fisher Scientific F20, a Helios FIB/SEM and a Teneo VolumeScope SEM as well as a variety of equipment for sample preparation including Leica tissue processors and ultramicrotomes, equipment for low temperature processing and multiple vacuum evaporators. The facility is funded by the Hospital and is utilized by faculty from across the research departments, providing world class imaging to the investigators at St. Jude. Staff members in the Center collaborate with investigators on a variety of research projects from standard tissue pathologies through three-dimensional imaging projects by electron tomography or volume SEM and have the opportunity to participate in development of techniques and protocols to drive St. Judes research forward. This position requires experience in biological electron microscopy. Skill sets in volume SEM (scanning block face SEM, array tomography, and FIB/SEM) and/or high pressure freezing and freeze substitution are highly desirable. Thanks, Cam
Camenzind G Robinson, PhD Director, Electron Microscopy Shared Resource Cell and Tissue Imaging Center St. Jude Childrens Research Hospital 262 Danny Thomas Place, Mail Stop 312 Memphis, TN 38105-3678
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Email: rvila-at-stanford.edu Name: Rafael A. Vila
Title-Subject: [Filtered] Stanford EM-X Symposium this Monday Dec. 7th (Cryo-EM & in situ-EM)
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Join us Monday December 7th at 8:00 AM (PST) for the second symposium in our new EM-X series.
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Email: talukder.m.alam-at-gmail.com Name: Talukder Alam
Title-Subject: [Filtered] Holder for t-EBSD/TKD
Message: Dear Microscope community,
I am looking for suggestions for a suitable TKD holder for a Quanta 650 system. If this forum is not the ideal platform I am available to communicate off-track.
I would also like to know about your experience with the data analysis regards the TKD. Was the existing EBSD software able to do the analysis?
Appreciate all the help! Regards, Talukder Alam
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You should check with your EBSD provider to make sure you have all of the needed software. My lab has used Oxford Aztec since 2.0. I believe t EBSD acquisition since Aztec 2.0 and Chanel 5 for analysis is possible. These days we use Aztec crystal for processing.
As for a sample holder, you may either make one or purchase a readily available holder. I do not have a recommendation for a quanta. It will depend on how much lens to stage clearance you have. One of my lab users has the Bruker t EBSD holder. It is a really nice unit but was a tight fit in the FEI xl30 Sirion. No problem to fit in our zeiss sigma.
Greg Baty Manager, CEMN Portland State University
Sent from my iPhone
} On Dec 4, 2020, at 4:42 AM, microscopy.listserver-at-gmail.com wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: talukder.m.alam-at-gmail.com } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/ } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } talukder.m.alam-at-gmail.com, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers } can benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: talukder.m.alam-at-gmail.com Name: Talukder Alam } } Title-Subject: [Filtered] Holder for t-EBSD/TKD } } Message: Dear Microscope community, } } I am looking for suggestions for a suitable TKD holder for a Quanta 650 system. If this forum is not } the ideal platform I am available to communicate off-track. } } I would also like to know about your experience with the data analysis regards the TKD. Was the } existing EBSD software able to do the analysis? } } Appreciate all the help! } Regards, } Talukder Alam } } } Login Host: 128.46.207.92 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 11, 53 -- From microscopy.listserver-at-gmail.com Fri Dec 4 06:38:56 2020 } 11, 53 -- Received: from mail-il1-f173.google.com (mail-il1-f173.google.com [209.85.166.173]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0B4Ccu2Z023419 } 11, 53 -- for {microscopy-at-microscopy.com} ; Fri, 4 Dec 2020 06:38:56 -0600 } 11, 53 -- Received: by mail-il1-f173.google.com with SMTP id b8so5064195ila.13 } 11, 53 -- for {microscopy-at-microscopy.com} ; Fri, 04 Dec 2020 04:52:50 -0800 (PST) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=mmYmdQt517qB+31NOQxD3pkELR37k7a7EjexTbA7K/0=; } 11, 53 -- b=QZ35ytlub9dh2yV7eQkI+iggh5l4M5ZwBS4WZrNBjXguzGbTALsZ33gmzWxO0CwdNa } 11, 53 -- OD+BG/t1JjJgoSnXqArKDnLqhiQyD6fIfCZ8wkrlnkuit29ZZ9Z3E3MAhNPngnoVx7t5 } 11, 53 -- UCMFM8WC5g+b5PAW7pi3S2V1vA47fm1asJufoiKn2mR7tOwQZrRMr1UoRt+mTBxCF2Ic } 11, 53 -- N73AzouQuBF1uu32Xc8Wo6waWcj3reSXnIw12pYffoQSAeThCCCOXEFUmYb89gky2wuP } 11, 53 -- DHQkESDsdMZPI5sGBhBImWglnwMCziKxqFmZjQ/GjDaeg38spNaMmKICHFpLAjcxHqLB } 11, 53 -- vBHA== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=mmYmdQt517qB+31NOQxD3pkELR37k7a7EjexTbA7K/0=; } 11, 53 -- b=H2WqC304RhlfmYaZ8ge2BCsJPSzUsheJHn8+hG30un/97BMdpQpRivX5KAbzKofqBT } 11, 53 -- YpYr6hJvLCY2O0OUtE43RgBN+wozjTms6PPUJI8PUVnSGbP2W50Q6eDET42CW7R4GMx5 } 11, 53 -- +tfoEA3PG2pVYkHSamBxFbw/LRtk5CO5Cm7tKWVdaapk7cjoTT56txZB3Qweh33ZzfzU } 11, 53 -- eNfCUfpz6Edls/wlmV9UX3afjnlOvIDbZHN1jGb9S42/hRZf4x43CsoQaKYWSpDslVt6 } 11, 53 -- /syq8uwp4Lt1JtG/PGCt442wQuL8Bz9TUrawdy2FLO3Lt+syBk1iLo0FN/fkYT0K7a60 } 11, 53 -- ehTg== } 11, 53 -- X-Gm-Message-State: AOAM531NYyZGDgMO60DGUZthdAJdd6S3oFZitWTpfgxlAGbLX9TOYNdf } 11, 53 -- NNSWg817zm5J4NgU1slBznZ4Df4Rezk= } 11, 53 -- X-Google-Smtp-Source: ABdhPJzatHMh2jDDNwjeWtud4vqpMqfnmCGpDdDjb3OZddRj6uDcVF0hW3GGyVR6MBcW1x20r6VYfw== } 11, 53 -- X-Received: by 2002:a92:85d6:: with SMTP id f205mr6149276ilh.133.1607086369864; } 11, 53 -- Fri, 04 Dec 2020 04:52:49 -0800 (PST) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:b053:9464:4383:3a79]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id q5sm1710816ilg.62.2020.12.04.04.52.48 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 11, 53 -- Fri, 04 Dec 2020 04:52:48 -0800 (PST) } 11, 53 -- Subject: viaWWW: Holder for t-EBSD/TKD } 11, 53 -- References: {202012031629.0B3GTcEa029074-at-microscopy.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: {202012031629.0B3GTcEa029074-at-microscopy.com} } 11, 53 -- Message-ID: {d614de20-9447-37b1-1703-1282d86d102a-at-gmail.com} } 11, 53 -- Date: Fri, 4 Dec 2020 06:52:47 -0600 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:78.0) } 11, 53 -- Gecko/20100101 Thunderbird/78.5.1 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: {202012031629.0B3GTcEa029074-at-microscopy.com} } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
On 04/12/2020 13:42, microscopy.listserver-at-gmail.com wrote:
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If this forum is not } the ideal platform I am available to communicate off-track. } } I would also like to know about your experience with the data analysis regards the TKD. Was the } existing EBSD software able to do the analysis? } } Appreciate all the help! } Regards, } Talukder Alam } } } Login Host: 128.46.207.92 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } } ==============================Original Headers============================== } 11, 53 -- Frommicroscopy.listserver-at-gmail.com Fri Dec 4 06:38:56 2020 } 11, 53 -- Received: from mail-il1-f173.google.com (mail-il1-f173.google.com [209.85.166.173]) } 11, 53 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0B4Ccu2Z023419 } 11, 53 -- for {microscopy-at-microscopy.com} ; Fri, 4 Dec 2020 06:38:56 -0600 } 11, 53 -- Received: by mail-il1-f173.google.com with SMTP id b8so5064195ila.13 } 11, 53 -- for {microscopy-at-microscopy.com} ; Fri, 04 Dec 2020 04:52:50 -0800 (PST) } 11, 53 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=gmail.com; s=20161025; } 11, 53 -- h=subject:references:to:from:message-id:date:user-agent:mime-version } 11, 53 -- :in-reply-to:content-language:content-transfer-encoding; } 11, 53 -- bh=mmYmdQt517qB+31NOQxD3pkELR37k7a7EjexTbA7K/0=; } 11, 53 -- b=QZ35ytlub9dh2yV7eQkI+iggh5l4M5ZwBS4WZrNBjXguzGbTALsZ33gmzWxO0CwdNa } 11, 53 -- OD+BG/t1JjJgoSnXqArKDnLqhiQyD6fIfCZ8wkrlnkuit29ZZ9Z3E3MAhNPngnoVx7t5 } 11, 53 -- UCMFM8WC5g+b5PAW7pi3S2V1vA47fm1asJufoiKn2mR7tOwQZrRMr1UoRt+mTBxCF2Ic } 11, 53 -- N73AzouQuBF1uu32Xc8Wo6waWcj3reSXnIw12pYffoQSAeThCCCOXEFUmYb89gky2wuP } 11, 53 -- DHQkESDsdMZPI5sGBhBImWglnwMCziKxqFmZjQ/GjDaeg38spNaMmKICHFpLAjcxHqLB } 11, 53 -- vBHA== } 11, 53 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 11, 53 -- d=1e100.net; s=20161025; } 11, 53 -- h=x-gm-message-state:subject:references:to:from:message-id:date } 11, 53 -- :user-agent:mime-version:in-reply-to:content-language } 11, 53 -- :content-transfer-encoding; } 11, 53 -- bh=mmYmdQt517qB+31NOQxD3pkELR37k7a7EjexTbA7K/0=; } 11, 53 -- b=H2WqC304RhlfmYaZ8ge2BCsJPSzUsheJHn8+hG30un/97BMdpQpRivX5KAbzKofqBT } 11, 53 -- YpYr6hJvLCY2O0OUtE43RgBN+wozjTms6PPUJI8PUVnSGbP2W50Q6eDET42CW7R4GMx5 } 11, 53 -- +tfoEA3PG2pVYkHSamBxFbw/LRtk5CO5Cm7tKWVdaapk7cjoTT56txZB3Qweh33ZzfzU } 11, 53 -- eNfCUfpz6Edls/wlmV9UX3afjnlOvIDbZHN1jGb9S42/hRZf4x43CsoQaKYWSpDslVt6 } 11, 53 -- /syq8uwp4Lt1JtG/PGCt442wQuL8Bz9TUrawdy2FLO3Lt+syBk1iLo0FN/fkYT0K7a60 } 11, 53 -- ehTg== } 11, 53 -- X-Gm-Message-State: AOAM531NYyZGDgMO60DGUZthdAJdd6S3oFZitWTpfgxlAGbLX9TOYNdf } 11, 53 -- NNSWg817zm5J4NgU1slBznZ4Df4Rezk= } 11, 53 -- X-Google-Smtp-Source: ABdhPJzatHMh2jDDNwjeWtud4vqpMqfnmCGpDdDjb3OZddRj6uDcVF0hW3GGyVR6MBcW1x20r6VYfw== } 11, 53 -- X-Received: by 2002:a92:85d6:: with SMTP id f205mr6149276ilh.133.1607086369864; } 11, 53 -- Fri, 04 Dec 2020 04:52:49 -0800 (PST) } 11, 53 -- Received: from 96-65-115-77-static.hfc.comcastbusiness.net ([2603:300a:f04:7100:b053:9464:4383:3a79]) } 11, 53 -- by smtp.googlemail.com with ESMTPSA id q5sm1710816ilg.62.2020.12.04.04.52.48 } 11, 53 -- for {microscopy-at-microscopy.com} } 11, 53 -- (version=TLS1_3 cipher=TLS_AES_128_GCM_SHA256 bits=128/128); } 11, 53 -- Fri, 04 Dec 2020 04:52:48 -0800 (PST) } 11, 53 -- Subject: viaWWW: Holder for t-EBSD/TKD } 11, 53 -- References: {202012031629.0B3GTcEa029074-at-microscopy.com} } 11, 53 -- To: MicroscopyListServer-Forward {microscopy-at-microscopy.com} } 11, 53 -- From: MIcroscopyListserver {microscopy.listserver-at-gmail.com} } 11, 53 -- X-Forwarded-Message-Id: {202012031629.0B3GTcEa029074-at-microscopy.com} } 11, 53 -- Message-ID: {d614de20-9447-37b1-1703-1282d86d102a-at-gmail.com} } 11, 53 -- Date: Fri, 4 Dec 2020 06:52:47 -0600 } 11, 53 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.14; rv:78.0) } 11, 53 -- Gecko/20100101 Thunderbird/78.5.1 } 11, 53 -- MIME-Version: 1.0 } 11, 53 -- In-Reply-To: {202012031629.0B3GTcEa029074-at-microscopy.com} } 11, 53 -- Content-Type: text/plain; charset=windows-1252; format=flowed } 11, 53 -- Content-Language: en-US } 11, 53 -- Content-Transfer-Encoding: 7bit } ==============================End of - Headers==============================
-- -- With kind regards, Gert ten Brink Dept. Nanostructured Materials and Interfaces Zernike Institute for Advanced Materials University of Groningen Nijenborgh 4 9747 AG Groningen The Netherlands Room 5113-213 phone: +31(0)503634889; fax:+31(0)503634881 e-mail:g.h.ten.brink-at-rug.nl; http://www.rug.nl/research/nanostructured-materials-interfaces/
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Email: anpenn-at-ncsu.edu Name: Aubrey Penn
Title-Subject: [Filtered] Undergraduate Experience Webinar and Scholarship Information Session
Message: Dear Microscopist, The Microscopy Society of America Student Council (StC) will be hosting the Undergraduate Experience webinar on December 10 for undergraduate students to learn about navigating undergraduate research and gather information about the MSA Undergraduate Scholarship (https://www.microscopy.org/awards/scholarship.cfm). Our invited panelists include previous scholarship winners and an experienced PI to undergraduate researchers. Participants can register at the following link to receive reminders and submit questions in advance of the webinar (https://forms.gle/wpSk3CuKbf2gQg739), and join via Zoom on Thursday, December 10 at 12 PM ET. Participants will need to register to join us on Zoom and have the opportunity to ask questions during the panel discussion. The webinar will also be live streamed on our Youtube page (https://www.youtube.com/channel/UC091t-wxZm0-4neu1jU7Log/).
StC is excited to announce that any MSA member who attends all webinars in this years Webinar Series will be entered in a drawing to win a StC merchandise package including a T-shirt, mug and more!
Our panelists are: Will Bowman, PhD Assistant Professor, UC Irvine
Noah Schnitzer Graduate Student, Cornell University MSA Undergraduate Scholarship Awardee
Jackson Spurling MSA Student Council Treasurer Undergraduate Researcher at UT Knoxville
Any questions can be directed to studentcouncil-at-microscopy.org. Sincerely, Aubrey Penn President Elect, MSA StC
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I would appreciate if you can give me some input on standard practice concerning co-authorship on TEM data collection by the staff. Is it appropriate for the staff to ask for co-authorship if the TEM staff collects the TEM data and the TEM images are used in the publication? The TEM user only pays the machine time, not the staff labor time. There is no data analysis involved. Or in this case, the user should just acknowledge the staff with funding information in the acknowledgement?
Thanks very much Yan Xin FSU
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This is an interesting question where I feel that there is a lot of confusion. In an academic setting the question of funding, or who pays for what, isn't really relevant. The only question that needs to be answered is if a person has contributed "enough" to the science be identified as an author. In our lab we try to follow the Vancouver recommendations:
http://www.icmje.org/icmje-recommendations.pdf
which give the following guidelines:
"2. Who Is an Author? The ICMJE recommends that authorship be based on the following 4 criteria: 1. _Substantial contributions to_ the conception or design of the work; _or the acquisition, analysis, or interpretation of data for the work_; AND 2. Drafting the work or revising it critically for important intellectual content; AND 3. Final approval of the version to be published; AND 4. Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
[...]
The criteria are not intended for use as a means to disqualify colleagues from authorship who otherwise meet authorship criteria by denying them the opportunity to meet criterion #s 2 or 3.Therefore, all individuals who meet the first criterion should have the opportunity to participate in the review,drafting, and final approval of the manuscript."
For the case that you are describing I believe the underlined part is the most relevant. As you see, any person who has given substantial contribution to acquisition of data "for the work" should be recognized as an author. Any data that is included in the manuscript would have to fall under the heading of "for the work", and a person who in actual fact was alone responsible for acquiring that data would necessarily have to be recognized as giving a "substantial contribution" to the acquisition. So my conclusion would be that yes, it is usually appropriate for staff to be included in the list of authors.
On a more general note, I would like to stress that in my opinion anything beyond the very simplest experiments in TEM require a level of scientific understanding that goes beyond a "technical" task. Decisions must be made on sample preparation and design, finding appropriate projections for diffraction and imaging, in STEM deciding on detectors and illumination conditions to use, in EELS deciding on which edges to select to find the information needed, in EDS understanding if absorption and fluorescence is an issue, and multiple other small and large decisions taken on the fly during the experiment. Many of these are things I expect my students to describe and discuss in their thesis, and if they don't I would conclude that they have not done a proper scientific job. Personally I would have been hesitant to submit a paper without including the person actually doing this job; who is then ensuring that what we write is correct?
Finally, I have never understood the impulse that some people have to limit the author list as much as possible. Of course, we shouldn't include people improperly, but neither should we look for ways of preventing people from becoming authors. That would very easily create a culture where people are guarded and careful about where they help out, and may even foster an atmosphere of mistrust and feelings of being underappreciated. Personally I would much rather err on the side of being too generous, than risk leaving out people who in reality should have been included. If people generally feel secure in the knowledge that their contributions will be acknowledged properly, they will be more inclined to help out, and may in time also more easily feel that they can forgo co-authorships for what they consider "trivial" tasks. This makes for a more healthy scientific environment.
Best regards Øystein
- Øystein Prytz, PhD | Professor of Physics | Mobile: +47 93201512 University of Oslo | Office: +47 22840684 oystein.prytz-at-fys.uio.no | http://folk.uio.no/oysteinp/group
On 07.12.2020 21:35, xin-at-magnet.fsu.edu wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, All, } } I would appreciate if you can give me some input on standard practice } concerning co-authorship on TEM data collection by the staff. } Is it appropriate for the staff to ask for co-authorship if the TEM staff } collects the TEM data and the TEM images are used in the publication? The } TEM user only pays the machine time, not the staff labor time. There is } no data analysis involved. } Or in this case, the user should just acknowledge the staff with funding } information in the acknowledgement? } } Thanks very much } Yan Xin } FSU } } } } ==============================Original Headers============================== } 5, 110 -- From xin-at-magnet.fsu.edu Mon Dec 7 14:31:50 2020 } 5, 110 -- Received: from NAM10-MW2-obe.outbound.protection.outlook.com (mail-mw2nam10on2060.outbound.protection.outlook.com [40.107.94.60]) } 5, 110 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0B7KVmoB026243 } 5, 110 -- for {Microscopy-at-microscopy.com} ; Mon, 7 Dec 2020 14:31:49 -0600 } 5, 110 -- ARC-Seal: i=1; a=rsa-sha256; s=arcselector9901; d=microsoft.com; cv=none; } 5, 110 -- b=dVY6cG1/OpjQQuUsM7APkyrqvLtqyLnKKXwXrqmCfJkwDpInXWXz9m7VOB2FpyMuHeaRz1h54lWyjCS2Zln7znvKNwfezGgUZ9lo7FrUz+n8BGkjyzf5TFuxu1oDbQwq8siRjzpumyqZp+iThG5OLcCe9CxpU6RLuiZZXRemqQ/nsYFx8TxzhxQYUF3zkdyICxRzrlSY8y1AeSbOWSelAn9yEsStseDhAusVcj90EQrnOEuKX3BUreMPj+GAdyu/8q/zZoMRqgLnXjXm4r4MsSbUie+HQZM5HhlXBGAdIf7Av7N2YwaVSInjc2RnCEBEf56N2qB7X9g4DLjf7hbgeg== } 5, 110 -- ARC-Message-Signature: i=1; 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-- Øystein Prytz, PhD | Professor of Physics | Mobile: +47 93201512 University of Oslo | Office: +47 22840684 oystein.prytz-at-fys.uio.no | http://folk.uio.no/oysteinp/group
==============================Original Headers============================== 24, 32 -- From oystein.prytz-at-fys.uio.no Tue Dec 8 01:39:50 2020 24, 32 -- Received: from mail-out02.uio.no (mail-out02.uio.no [129.240.10.71]) 24, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0B87dn5f016544 24, 32 -- for {microscopy-at-microscopy.com} ; Tue, 8 Dec 2020 01:39:49 -0600 24, 32 -- Received: from mail-mx05.uio.no ([129.240.10.49]) 24, 32 -- by mail-out02.uio.no with esmtps (TLS1.2) tls TLS_ECDHE_RSA_WITH_AES_256_GCM_SHA384 24, 32 -- (Exim 4.93.0.4) 24, 32 -- (envelope-from {oystein.prytz-at-fys.uio.no} ) 24, 32 -- id 1kmXoo-0002aO-Sc 24, 32 -- for microscopy-at-microscopy.com; Tue, 08 Dec 2020 08:53:54 +0100 24, 32 -- Received: from cm-84.212.212.95.getinternet.no ([84.212.212.95] helo=[192.168.0.237]) 24, 32 -- by mail-mx05.uio.no with esmtpsa (TLS1.2:ECDHE-RSA-AES128-GCM-SHA256:128) 24, 32 -- user oysteinp (Exim 4.93.0.4) 24, 32 -- (envelope-from {oystein.prytz-at-fys.uio.no} ) 24, 32 -- id 1kmXom-0004DJ-MJ; Tue, 08 Dec 2020 08:53:54 +0100 24, 32 -- From: =?UTF-8?Q?=c3=98ystein_Prytz?= {oystein.prytz-at-fys.uio.no} 24, 32 -- Subject: Re: [Microscopy] inquiry on standard practice on co-authorship 24, 32 -- request 24, 32 -- To: Microscopy-at-microscopy.com 24, 32 -- References: {202012072035.0B7KZcUn030072-at-microscopy.com} 24, 32 -- Message-ID: {68524f77-cf22-b733-cc0f-f7095c338646-at-fys.uio.no} 24, 32 -- Date: Tue, 8 Dec 2020 08:53:53 +0100 24, 32 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; Win64; x64; rv:68.0) Gecko/20100101 24, 32 -- Thunderbird/68.12.1 24, 32 -- MIME-Version: 1.0 24, 32 -- In-Reply-To: {202012072035.0B7KZcUn030072-at-microscopy.com} 24, 32 -- Content-Type: text/plain; charset=utf-8; format=flowed 24, 32 -- Content-Language: en-US 24, 32 -- Content-Transfer-Encoding: 8bit 24, 32 -- X-UiO-SPF-Received: Received-SPF: neutral (mail-mx05.uio.no: 84.212.212.95 is neither permitted nor denied by domain of fys.uio.no) client-ip=84.212.212.95; envelope-from=oystein.prytz-at-fys.uio.no; helo=[192.168.0.237]; 24, 32 -- X-UiO-Spam-info: not spam, SpamAssassin (score=-5.3, required=5.0, autolearn=disabled, AWL=-0.253,NICE_REPLY_A=-0.001,UIO_MAIL_IS_INTERNAL=-5, uiobl=NO, uiouri=NO) 24, 32 -- X-UiO-Scanned: A295906D791B2240671FAF6DD9D830FECAA362EB ==============================End of - Headers==============================
The subjective manner of choosing authorship recognition has always been too far open to interpretation and bias, in my opinion.
In my Lab Manager job for a university decades ago, I collected all of the data, from live animal sedation through perfusion, fixation, staining, blocking and embedding, sectioning, UA-LC staining, imaging, darkroom work, the Methods section, and all plates and posters labeled and assembled (remember dry mount?). I didn't even receive acknowledgement, since "I was being paid to do that" (as a technician). By that logic, he's paid to be a Principal Investigator, but I do understand that he does mentor the graduate student author and assists with editing. To this day, I believe he wanted to claim only academic creative contributions (versus technical) as justification for authorship. He also did not include me when I performed immunohistochemistry and very skill-intensive serial sectioning montage posters. Personally, I believe he thinks it adds more prestige to have only one or two authors on his papers.
In industry, we often had several co-authors, and our department head included himself as last author for no other reason than he had to read and approve the final result. This was company policy, and therefore his job. He has over 700 papers to his name. Isn't that prestigious? I recently read an official document justifying my label of that behavior as completely inappropriate. Who was going to stop him, though? He can kill the entire paper with a word.
I'm glad to see more precise definitions for authorship being published, although most principal investigators will go with policies based on their own opinion.
If it sounds like a gripe, it's because I've experienced both sides of the coin. One or two authors as one standard and seven to twelve as another standard. I think reasonable acknowledgment without bloat is the way to go.
My opinion, for what it's worth. ~Gregg
Gregg Sobocinski Microscope Imaging Suite, Managing Director University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
On Tue, Dec 8, 2020 at 2:59 AM {oystein.prytz-at-fys.uio.no} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello! } } This is an interesting question where I feel that there is a lot of } confusion. In an academic setting the question of funding, or who pays } for what, isn't really relevant. The only question that needs to be } answered is if a person has contributed "enough" to the science be } identified as an author. In our lab we try to follow the Vancouver } recommendations: } } http://www.icmje.org/icmje-recommendations.pdf } } which give the following guidelines: } } "2. Who Is an Author? } The ICMJE recommends that authorship be based on the following 4 } criteria: } 1. _Substantial contributions to_ the conception or design of the } work; _or the acquisition, analysis, or interpretation of data for } the work_; AND } 2. Drafting the work or revising it critically for important } intellectual content; AND } 3. Final approval of the version to be published; AND } 4. Agreement to be accountable for all aspects of the work in } ensuring that questions related to the accuracy or integrity of any } part of the work are appropriately investigated and resolved. } } [...] } } The criteria are not intended for use as a means to disqualify } colleagues from authorship who otherwise meet authorship criteria by } denying them the opportunity to meet criterion #s 2 or 3.Therefore, } all individuals who meet the first criterion should have the } opportunity to participate in the review,drafting, and final } approval of the manuscript." } } For the case that you are describing I believe the underlined part is } the most relevant. As you see, any person who has given substantial } contribution to acquisition of data "for the work" should be recognized } as an author. Any data that is included in the manuscript would have to } fall under the heading of "for the work", and a person who in actual } fact was alone responsible for acquiring that data would necessarily } have to be recognized as giving a "substantial contribution" to the } acquisition. So my conclusion would be that yes, it is usually } appropriate for staff to be included in the list of authors. } } On a more general note, I would like to stress that in my opinion } anything beyond the very simplest experiments in TEM require a level of } scientific understanding that goes beyond a "technical" task. Decisions } must be made on sample preparation and design, finding appropriate } projections for diffraction and imaging, in STEM deciding on detectors } and illumination conditions to use, in EELS deciding on which edges to } select to find the information needed, in EDS understanding if } absorption and fluorescence is an issue, and multiple other small and } large decisions taken on the fly during the experiment. Many of these } are things I expect my students to describe and discuss in their thesis, } and if they don't I would conclude that they have not done a proper } scientific job. Personally I would have been hesitant to submit a paper } without including the person actually doing this job; who is then } ensuring that what we write is correct? } } Finally, I have never understood the impulse that some people have to } limit the author list as much as possible. Of course, we shouldn't } include people improperly, but neither should we look for ways of } preventing people from becoming authors. That would very easily create a } culture where people are guarded and careful about where they help out, } and may even foster an atmosphere of mistrust and feelings of being } underappreciated. Personally I would much rather err on the side of } being too generous, than risk leaving out people who in reality should } have been included. If people generally feel secure in the knowledge } that their contributions will be acknowledged properly, they will be } more inclined to help out, and may in time also more easily feel that } they can forgo co-authorships for what they consider "trivial" tasks. } This makes for a more healthy scientific environment. } } Best regards } Øystein } } - } Øystein Prytz, PhD | } Professor of Physics | Mobile: +47 93201512 } University of Oslo | Office: +47 22840684 } oystein.prytz-at-fys.uio.no | http://folk.uio.no/oysteinp/group } } } On 07.12.2020 21:35, xin-at-magnet.fsu.edu wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi, All, } } } } I would appreciate if you can give me some input on standard practice } } concerning co-authorship on TEM data collection by the staff. } } Is it appropriate for the staff to ask for co-authorship if the TEM staff } } collects the TEM data and the TEM images are used in the publication? The } } TEM user only pays the machine time, not the staff labor time. There is } } no data analysis involved. } } Or in this case, the user should just acknowledge the staff with funding } } information in the acknowledgement? } } } } Thanks very much } } Yan Xin } } FSU } } } } =====End of - Headers==============================
==============================Original Headers============================== 10, 44 -- From greggps-at-umich.edu Tue Dec 8 14:29:40 2020 10, 44 -- Received: from mail-yb1-f169.google.com (mail-yb1-f169.google.com [209.85.219.169]) 10, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0B8KTehg020955 10, 44 -- for {microscopy-at-microscopy.com} ; Tue, 8 Dec 2020 14:29:40 -0600 10, 44 -- Received: by mail-yb1-f169.google.com with SMTP id v67so4891728ybi.1 10, 44 -- for {microscopy-at-microscopy.com} ; Tue, 08 Dec 2020 12:43:48 -0800 (PST) 10, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 44 -- d=umich.edu; s=google-2016-06-03; 10, 44 -- h=mime-version:references:in-reply-to:from:date:message-id:subject:to 10, 44 -- :content-transfer-encoding; 10, 44 -- bh=KzFfLVSa2xSFjW1xezLX8LIlPT+oewq9arOmXv/fwhw=; 10, 44 -- b=ijUyIA6wylS5ep7aSD/4fITy9GA/VaeTqZrVig5sNUa0br4JicTvRh5caRLfSHy6e7 10, 44 -- siDsiG+gYkQ/6sH3RXPMpA4P8ULQq0a3xJ6grra8e59pUvABQu5Dhp4ZZ1+qR8FMbUDU 10, 44 -- jZrIxRmmtZ9xWEcJQjVNYYDGaHPK41q3YwPIPdabtyuKVYRQQakVQQhIFyAr4o4zhofn 10, 44 -- jbtPupU1QkZ8cNFyY0SVWD/JtjkQUqZjNo0FWCUGpmbYYuJbTZKPFIdAxISl3pGjg2Yu 10, 44 -- DMvzjxbMayXTGl3TpD5L0/eUDLrqY0rGapKFX+7Dpxo+mJvVM+jYQ0ovfCneFv+COPE2 10, 44 -- mOcQ== 10, 44 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 44 -- d=1e100.net; s=20161025; 10, 44 -- h=x-gm-message-state:mime-version:references:in-reply-to:from:date 10, 44 -- :message-id:subject:to:content-transfer-encoding; 10, 44 -- bh=KzFfLVSa2xSFjW1xezLX8LIlPT+oewq9arOmXv/fwhw=; 10, 44 -- b=RXMCqwu0/aZnZdoNph2k+SbbuYcXNaQ801fkawuWzCgGhd3Z+WDYPHgq85PrqeCAFe 10, 44 -- iLCiqub+538+JGm4+MIT9XTxzIwGCsPipcBWrBYEkiLo5/TeBfq1+QnmzV92jDCjuivI 10, 44 -- wo1Q69gWBx0n4QDzBbSLXVW6qOHYgD9MeR4vlsKC435OH0P8aVO94g4TKX1Yk0M0ceu6 10, 44 -- w/eg3H7Fz31KrHW+3qtsVnKfbD0fqFbw8kSA4WJj+YX45pF1fvw6z6il4OA2G11/p/Fq 10, 44 -- VLAzQ9jDSvBoQ8mXjX9mxyN0fYsBy2KDC5EFVnZ5l5/hfOnlLB1VUzG6pkbWXk1b7u5e 10, 44 -- aTIg== 10, 44 -- X-Gm-Message-State: AOAM532ipnlEgz7FQ6W9qHdpE/SNm/TOR7afPa5qRgvvD61LljnBiFx1 10, 44 -- 5wpL/syyk2BPhoXpDX7LUHHkvUM6wlW1Jj4Uac7tJoayR+w= 10, 44 -- X-Google-Smtp-Source: ABdhPJwcOQd6SCxKo12iaR3YHin4MZtFP+w1NCn5kc1PSjnkrQnXO6tFb6z6BZocQj1y0Up1ePmQgIH4/ndWHdMGGMs= 10, 44 -- X-Received: by 2002:a25:be89:: with SMTP id i9mr31094258ybk.35.1607460227892; 10, 44 -- Tue, 08 Dec 2020 12:43:47 -0800 (PST) 10, 44 -- MIME-Version: 1.0 10, 44 -- References: {202012080744.0B87irkU021566-at-microscopy.com} {CABg+575vSM_MtEZB=VZW-MJTL5Mi6PwbSCp52oeZWFTSdNLf=Q-at-mail.gmail.com} 10, 44 -- In-Reply-To: {CABg+575vSM_MtEZB=VZW-MJTL5Mi6PwbSCp52oeZWFTSdNLf=Q-at-mail.gmail.com} 10, 44 -- From: Gregg Sobocinski {greggps-at-umich.edu} 10, 44 -- Date: Tue, 8 Dec 2020 15:43:11 -0500 10, 44 -- Message-ID: {CABg+574GG7uzR+M+eWrxCt_cBZg52O-dA=fc0BP4dKpu3Rzr0g-at-mail.gmail.com} 10, 44 -- Subject: Fwd: [Microscopy] Re: inquiry on standard practice on co-authorship 10, 44 -- To: microscopy-at-microscopy.com 10, 44 -- Content-Type: text/plain; charset="UTF-8" 10, 44 -- Content-Transfer-Encoding: 8bit 10, 44 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 0B8KTehg020955 ==============================End of - Headers==============================
I think that your experience unfortunately is quite common, and in my opinion that way of doing it is inappropriate. A mention in the acknowledgements should be the very minimum for what you describe. Regarding who-gets-paid-for-what you are entirely on point: we are all doing the job we are being paid for, so what?
I should hasten to say that in my first email I was a little imprecise: fulfilling the first of the Vancouver criteria is not _alone_ enough to qualify as a co-author, but qualifies to be invited to contribute on the second (and third) criterion, and thereby further qualify as a co-author.
On the point of department heads or lab-leaders being co-authors as a matter of policy: In my opinion this is not an acceptable way of doing it. You might very well have a policy that the department head should get the opportunity to be involved in such a way it qualifies a co-author, but it is _that actual involvement_ that qualifies him/her as a co-author, not the policy! That person should still fulfill the same authorship criteria as everyone else (e.g. the Vancouver recommendations). Whether or not he/she actually does fulfill those criteria has to be evaluated in each specific case.
I don't have too much experience with such policies, I don't think they are too common in Norway (or the Nordic countries in general). However, as a student I have experienced it in some international collaborations. In those cases I think it turned out to improve the final papers quite a bit. Perhaps I was lucky, but what the policy in those cases did was to secure input from very experienced scientists to my work. If implemented properly, such policies could also be of benefit to junior researchers.
Best regards Øystein
On 08.12.2020 21:34, greggps-at-umich.edu wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } The subjective manner of choosing authorship recognition has always } been too far open to interpretation and bias, in my opinion. } } In my Lab Manager job for a university decades ago, I collected all of } the data, from live animal sedation through perfusion, fixation, } staining, blocking and embedding, sectioning, UA-LC staining, imaging, } darkroom work, the Methods section, and all plates and posters labeled } and assembled (remember dry mount?). I didn't even receive } acknowledgement, since "I was being paid to do that" (as a } technician). By that logic, he's paid to be a Principal Investigator, } but I do understand that he does mentor the graduate student author } and assists with editing. To this day, I believe he wanted to claim } only academic creative contributions (versus technical) as } justification for authorship. He also did not include me when I } performed immunohistochemistry and very skill-intensive serial } sectioning montage posters. Personally, I believe he thinks it adds } more prestige to have only one or two authors on his papers. } } In industry, we often had several co-authors, and our department head } included himself as last author for no other reason than he had to } read and approve the final result. This was company policy, and } therefore his job. He has over 700 papers to his name. Isn't that } prestigious? I recently read an official document justifying my label } of that behavior as completely inappropriate. Who was going to stop } him, though? He can kill the entire paper with a word. } } I'm glad to see more precise definitions for authorship being } published, although most principal investigators will go with policies } based on their own opinion. } } If it sounds like a gripe, it's because I've experienced both sides of } the coin. One or two authors as one standard and seven to twelve as } another standard. I think reasonable acknowledgment without bloat is } the way to go. } } My opinion, for what it's worth. } ~Gregg } } Gregg Sobocinski } Microscope Imaging Suite, Managing Director } University of Michigan, MCDB Dept. } Ann Arbor, Michigan } USA } } } On Tue, Dec 8, 2020 at 2:59 AM {oystein.prytz-at-fys.uio.no} wrote: } } } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hello! } } } } This is an interesting question where I feel that there is a lot of } } confusion. In an academic setting the question of funding, or who pays } } for what, isn't really relevant. The only question that needs to be } } answered is if a person has contributed "enough" to the science be } } identified as an author. In our lab we try to follow the Vancouver } } recommendations: } } } } http://www.icmje.org/icmje-recommendations.pdf } } } } which give the following guidelines: } } } } "2. Who Is an Author? } } The ICMJE recommends that authorship be based on the following 4 } } criteria: } } 1. _Substantial contributions to_ the conception or design of the } } work; _or the acquisition, analysis, or interpretation of data for } } the work_; AND } } 2. Drafting the work or revising it critically for important } } intellectual content; AND } } 3. Final approval of the version to be published; AND } } 4. Agreement to be accountable for all aspects of the work in } } ensuring that questions related to the accuracy or integrity of any } } part of the work are appropriately investigated and resolved. } } } } [...] } } } } The criteria are not intended for use as a means to disqualify } } colleagues from authorship who otherwise meet authorship criteria by } } denying them the opportunity to meet criterion #s 2 or 3.Therefore, } } all individuals who meet the first criterion should have the } } opportunity to participate in the review,drafting, and final } } approval of the manuscript." } } } } For the case that you are describing I believe the underlined part is } } the most relevant. As you see, any person who has given substantial } } contribution to acquisition of data "for the work" should be recognized } } as an author. Any data that is included in the manuscript would have to } } fall under the heading of "for the work", and a person who in actual } } fact was alone responsible for acquiring that data would necessarily } } have to be recognized as giving a "substantial contribution" to the } } acquisition. So my conclusion would be that yes, it is usually } } appropriate for staff to be included in the list of authors. } } } } On a more general note, I would like to stress that in my opinion } } anything beyond the very simplest experiments in TEM require a level of } } scientific understanding that goes beyond a "technical" task. Decisions } } must be made on sample preparation and design, finding appropriate } } projections for diffraction and imaging, in STEM deciding on detectors } } and illumination conditions to use, in EELS deciding on which edges to } } select to find the information needed, in EDS understanding if } } absorption and fluorescence is an issue, and multiple other small and } } large decisions taken on the fly during the experiment. Many of these } } are things I expect my students to describe and discuss in their thesis, } } and if they don't I would conclude that they have not done a proper } } scientific job. Personally I would have been hesitant to submit a paper } } without including the person actually doing this job; who is then } } ensuring that what we write is correct? } } } } Finally, I have never understood the impulse that some people have to } } limit the author list as much as possible. Of course, we shouldn't } } include people improperly, but neither should we look for ways of } } preventing people from becoming authors. That would very easily create a } } culture where people are guarded and careful about where they help out, } } and may even foster an atmosphere of mistrust and feelings of being } } underappreciated. Personally I would much rather err on the side of } } being too generous, than risk leaving out people who in reality should } } have been included. If people generally feel secure in the knowledge } } that their contributions will be acknowledged properly, they will be } } more inclined to help out, and may in time also more easily feel that } } they can forgo co-authorships for what they consider "trivial" tasks. } } This makes for a more healthy scientific environment. } } } } Best regards } } Øystein } } } } - } } Øystein Prytz, PhD | } } Professor of Physics | Mobile: +47 93201512 } } University of Oslo | Office: +47 22840684 } } oystein.prytz-at-fys.uio.no | http://folk.uio.no/oysteinp/group } } } } } } On 07.12.2020 21:35, xin-at-magnet.fsu.edu wrote: } } } } } } } } } } } } ---------------------------------------------------------------------------- } } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } } ---------------------------------------------------------------------------- } } } } } } Hi, All, } } } } } } I would appreciate if you can give me some input on standard practice } } } concerning co-authorship on TEM data collection by the staff. } } } Is it appropriate for the staff to ask for co-authorship if the TEM staff } } } collects the TEM data and the TEM images are used in the publication? The } } } TEM user only pays the machine time, not the staff labor time. There is } } } no data analysis involved. } } } Or in this case, the user should just acknowledge the staff with funding } } } information in the acknowledgement? } } } } } } Thanks very much } } } Yan Xin } } } FSU } } } } } } =====End of - Headers============================== } } } ==============================Original Headers============================== } 10, 44 -- From greggps-at-umich.edu Tue Dec 8 14:29:40 2020 } 10, 44 -- Received: from mail-yb1-f169.google.com (mail-yb1-f169.google.com [209.85.219.169]) } 10, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0B8KTehg020955 } 10, 44 -- for {microscopy-at-microscopy.com} ; Tue, 8 Dec 2020 14:29:40 -0600 } 10, 44 -- Received: by mail-yb1-f169.google.com with SMTP id v67so4891728ybi.1 } 10, 44 -- for {microscopy-at-microscopy.com} ; Tue, 08 Dec 2020 12:43:48 -0800 (PST) } 10, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 44 -- d=umich.edu; s=google-2016-06-03; } 10, 44 -- h=mime-version:references:in-reply-to:from:date:message-id:subject:to } 10, 44 -- :content-transfer-encoding; } 10, 44 -- bh=KzFfLVSa2xSFjW1xezLX8LIlPT+oewq9arOmXv/fwhw=; } 10, 44 -- b=ijUyIA6wylS5ep7aSD/4fITy9GA/VaeTqZrVig5sNUa0br4JicTvRh5caRLfSHy6e7 } 10, 44 -- siDsiG+gYkQ/6sH3RXPMpA4P8ULQq0a3xJ6grra8e59pUvABQu5Dhp4ZZ1+qR8FMbUDU } 10, 44 -- jZrIxRmmtZ9xWEcJQjVNYYDGaHPK41q3YwPIPdabtyuKVYRQQakVQQhIFyAr4o4zhofn } 10, 44 -- jbtPupU1QkZ8cNFyY0SVWD/JtjkQUqZjNo0FWCUGpmbYYuJbTZKPFIdAxISl3pGjg2Yu } 10, 44 -- DMvzjxbMayXTGl3TpD5L0/eUDLrqY0rGapKFX+7Dpxo+mJvVM+jYQ0ovfCneFv+COPE2 } 10, 44 -- mOcQ== } 10, 44 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; } 10, 44 -- d=1e100.net; s=20161025; } 10, 44 -- h=x-gm-message-state:mime-version:references:in-reply-to:from:date } 10, 44 -- :message-id:subject:to:content-transfer-encoding; } 10, 44 -- bh=KzFfLVSa2xSFjW1xezLX8LIlPT+oewq9arOmXv/fwhw=; } 10, 44 -- b=RXMCqwu0/aZnZdoNph2k+SbbuYcXNaQ801fkawuWzCgGhd3Z+WDYPHgq85PrqeCAFe } 10, 44 -- iLCiqub+538+JGm4+MIT9XTxzIwGCsPipcBWrBYEkiLo5/TeBfq1+QnmzV92jDCjuivI } 10, 44 -- wo1Q69gWBx0n4QDzBbSLXVW6qOHYgD9MeR4vlsKC435OH0P8aVO94g4TKX1Yk0M0ceu6 } 10, 44 -- w/eg3H7Fz31KrHW+3qtsVnKfbD0fqFbw8kSA4WJj+YX45pF1fvw6z6il4OA2G11/p/Fq } 10, 44 -- VLAzQ9jDSvBoQ8mXjX9mxyN0fYsBy2KDC5EFVnZ5l5/hfOnlLB1VUzG6pkbWXk1b7u5e } 10, 44 -- aTIg== } 10, 44 -- X-Gm-Message-State: AOAM532ipnlEgz7FQ6W9qHdpE/SNm/TOR7afPa5qRgvvD61LljnBiFx1 } 10, 44 -- 5wpL/syyk2BPhoXpDX7LUHHkvUM6wlW1Jj4Uac7tJoayR+w= } 10, 44 -- X-Google-Smtp-Source: ABdhPJwcOQd6SCxKo12iaR3YHin4MZtFP+w1NCn5kc1PSjnkrQnXO6tFb6z6BZocQj1y0Up1ePmQgIH4/ndWHdMGGMs= } 10, 44 -- X-Received: by 2002:a25:be89:: with SMTP id i9mr31094258ybk.35.1607460227892; } 10, 44 -- Tue, 08 Dec 2020 12:43:47 -0800 (PST) } 10, 44 -- MIME-Version: 1.0 } 10, 44 -- References: {202012080744.0B87irkU021566-at-microscopy.com} {CABg+575vSM_MtEZB=VZW-MJTL5Mi6PwbSCp52oeZWFTSdNLf=Q-at-mail.gmail.com} } 10, 44 -- In-Reply-To: {CABg+575vSM_MtEZB=VZW-MJTL5Mi6PwbSCp52oeZWFTSdNLf=Q-at-mail.gmail.com} } 10, 44 -- From: Gregg Sobocinski {greggps-at-umich.edu} } 10, 44 -- Date: Tue, 8 Dec 2020 15:43:11 -0500 } 10, 44 -- Message-ID: {CABg+574GG7uzR+M+eWrxCt_cBZg52O-dA=fc0BP4dKpu3Rzr0g-at-mail.gmail.com} } 10, 44 -- Subject: Fwd: [Microscopy] Re: inquiry on standard practice on co-authorship } 10, 44 -- To: microscopy-at-microscopy.com } 10, 44 -- Content-Type: text/plain; charset="UTF-8" } 10, 44 -- Content-Transfer-Encoding: 8bit } 10, 44 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 0B8KTehg020955 } ==============================End of - Headers============================== }
-- Øystein Prytz, PhD | Professor of Physics | Mobile: +47 93201512 University of Oslo | Office: +47 22840684 oystein.prytz-at-fys.uio.no | http://folk.uio.no/oysteinp/group
==============================Original Headers============================== 13, 32 -- From oystein.prytz-at-fys.uio.no Wed Dec 9 02:43:58 2020 13, 32 -- Received: from mail-out01.uio.no (mail-out01.uio.no [129.240.10.50]) 13, 32 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0B98hvJP018570 13, 32 -- for {microscopy-at-microscopy.com} ; Wed, 9 Dec 2020 02:43:58 -0600 13, 32 -- Received: from mail-mx05.uio.no ([129.240.10.49]) 13, 32 -- by mail-out01.uio.no with esmtps (TLS1.2) tls TLS_ECDHE_RSA_WITH_AES_256_GCM_SHA384 13, 32 -- (Exim 4.93.0.4) 13, 32 -- (envelope-from {oystein.prytz-at-fys.uio.no} ) 13, 32 -- id 1kmvIT-0003WI-Rf; Wed, 09 Dec 2020 09:58:05 +0100 13, 32 -- Received: from da-cluster-p08-int.uio.no ([129.240.177.107] helo=[IPv6:2001:700:100:4026:e490:15a9:9304:7d6]) 13, 32 -- by mail-mx05.uio.no with esmtpsa (TLS1.2:ECDHE-RSA-AES128-GCM-SHA256:128) 13, 32 -- user oysteinp (Exim 4.93.0.4) 13, 32 -- (envelope-from {oystein.prytz-at-fys.uio.no} ) 13, 32 -- id 1kmvIR-000GyY-Mh; Wed, 09 Dec 2020 09:58:05 +0100 13, 32 -- Subject: Re: [Microscopy] Fwd: Re: inquiry on standard practice on 13, 32 -- co-authorship 13, 32 -- References: {202012082034.0B8KY5Bq025126-at-microscopy.com} 13, 32 -- From: =?UTF-8?Q?=c3=98ystein_Prytz?= {oystein.prytz-at-fys.uio.no} 13, 32 -- To: greggps-at-umich.edu 13, 32 -- Cc: Microscopy-at-microscopy.com 13, 32 -- Message-ID: {86cd9ccb-45f2-2314-743e-414f36df99ce-at-fys.uio.no} 13, 32 -- Date: Wed, 9 Dec 2020 09:58:02 +0100 13, 32 -- User-Agent: Mozilla/5.0 (Windows NT 10.0; Win64; x64; rv:68.0) Gecko/20100101 13, 32 -- Thunderbird/68.12.1 13, 32 -- MIME-Version: 1.0 13, 32 -- In-Reply-To: {202012082034.0B8KY5Bq025126-at-microscopy.com} 13, 32 -- Content-Type: text/plain; charset=utf-8; format=flowed 13, 32 -- Content-Language: en-US 13, 32 -- Content-Transfer-Encoding: 8bit 13, 32 -- X-UiO-SPF-Received: Received-SPF: neutral (mail-mx05.uio.no: 129.240.177.107 is neither permitted nor denied by domain of fys.uio.no) client-ip=129.240.177.107; envelope-from=oystein.prytz-at-fys.uio.no; helo=[IPv6:2001:700:100:4026:e490:15a9:9304:7d6]; 13, 32 -- X-UiO-Spam-info: not spam, SpamAssassin (score=-5.1, required=5.0, autolearn=disabled, AWL=-0.193,NICE_REPLY_A=-0.001,PDS_LITECOIN_ID=0.122,UIO_MAIL_IS_INTERNAL=-5, uiobl=NO, uiouri=NO) 13, 32 -- X-UiO-Scanned: 4C54CF0DB3C3949F62B6CAE16B0B2C6D3F210B44 ==============================End of - Headers==============================
you make a very good point. As a PhD student, my mentor even published my results without my name! And yes he can do it, he is all-powerful and you as a student simply have no right, the university is not a democracy. The same person also never included his technical assistant in his publication, although he couldn't publish anything without her, she was a very skillful technician. Another research group next door included all personel in all publication, independently of who did the real work (which may be questionable too but it doesn't hurt anybody, does it?).
I completely share you opinion: everyone involved in the paper should be mentionned. But scientists are human beings like others, with their ego problems and the complexity of human relationships as we know them.
Kind regards, Stephane
On Tuesday, December 8, 2020, 09:49:17 PM GMT+1, greggps-at-umich.edu {greggps-at-umich.edu} wrote:
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The subjective manner of choosing authorship recognition has always been too far open to interpretation and bias, in my opinion.
In my Lab Manager job for a university decades ago, I collected all of the data, from live animal sedation through perfusion, fixation, staining, blocking and embedding, sectioning, UA-LC staining, imaging, darkroom work, the Methods section, and all plates and posters labeled and assembled (remember dry mount?). I didn't even receive acknowledgement, since "I was being paid to do that" (as a technician). By that logic, he's paid to be a Principal Investigator, but I do understand that he does mentor the graduate student author and assists with editing. To this day, I believe he wanted to claim only academic creative contributions (versus technical) as justification for authorship. He also did not include me when I performed immunohistochemistry and very skill-intensive serial sectioning montage posters. Personally, I believe he thinks it adds more prestige to have only one or two authors on his papers.
In industry, we often had several co-authors, and our department head included himself as last author for no other reason than he had to read and approve the final result. This was company policy, and therefore his job. He has over 700 papers to his name. Isn't that prestigious? I recently read an official document justifying my label of that behavior as completely inappropriate. Who was going to stop him, though? He can kill the entire paper with a word.
I'm glad to see more precise definitions for authorship being published, although most principal investigators will go with policies based on their own opinion.
If it sounds like a gripe, it's because I've experienced both sides of the coin. One or two authors as one standard and seven to twelve as another standard. I think reasonable acknowledgment without bloat is the way to go.
My opinion, for what it's worth. ~Gregg
Gregg Sobocinski Microscope Imaging Suite, Managing Director University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
On Tue, Dec 8, 2020 at 2:59 AM {oystein.prytz-at-fys.uio.no} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hello! } } This is an interesting question where I feel that there is a lot of } confusion. In an academic setting the question of funding, or who pays } for what, isn't really relevant. The only question that needs to be } answered is if a person has contributed "enough" to the science be } identified as an author. In our lab we try to follow the Vancouver } recommendations: } } http://www.icmje.org/icmje-recommendations.pdf } } which give the following guidelines: } } "2. Who Is an Author? } The ICMJE recommends that authorship be based on the following 4 } criteria: } 1. _Substantial contributions to_ the conception or design of the } work; _or the acquisition, analysis, or interpretation of data for } the work_; AND } 2. Drafting the work or revising it critically for important } intellectual content; AND } 3. Final approval of the version to be published; AND } 4. Agreement to be accountable for all aspects of the work in } ensuring that questions related to the accuracy or integrity of any } part of the work are appropriately investigated and resolved. } } [...] } } The criteria are not intended for use as a means to disqualify } colleagues from authorship who otherwise meet authorship criteria by } denying them the opportunity to meet criterion #s 2 or 3.Therefore, } all individuals who meet the first criterion should have the } opportunity to participate in the review,drafting, and final } approval of the manuscript." } } For the case that you are describing I believe the underlined part is } the most relevant. As you see, any person who has given substantial } contribution to acquisition of data "for the work" should be recognized } as an author. Any data that is included in the manuscript would have to } fall under the heading of "for the work", and a person who in actual } fact was alone responsible for acquiring that data would necessarily } have to be recognized as giving a "substantial contribution" to the } acquisition. So my conclusion would be that yes, it is usually } appropriate for staff to be included in the list of authors. } } On a more general note, I would like to stress that in my opinion } anything beyond the very simplest experiments in TEM require a level of } scientific understanding that goes beyond a "technical" task. Decisions } must be made on sample preparation and design, finding appropriate } projections for diffraction and imaging, in STEM deciding on detectors } and illumination conditions to use, in EELS deciding on which edges to } select to find the information needed, in EDS understanding if } absorption and fluorescence is an issue, and multiple other small and } large decisions taken on the fly during the experiment. Many of these } are things I expect my students to describe and discuss in their thesis, } and if they don't I would conclude that they have not done a proper } scientific job. Personally I would have been hesitant to submit a paper } without including the person actually doing this job; who is then } ensuring that what we write is correct? } } Finally, I have never understood the impulse that some people have to } limit the author list as much as possible. Of course, we shouldn't } include people improperly, but neither should we look for ways of } preventing people from becoming authors. That would very easily create a } culture where people are guarded and careful about where they help out, } and may even foster an atmosphere of mistrust and feelings of being } underappreciated. Personally I would much rather err on the side of } being too generous, than risk leaving out people who in reality should } have been included. If people generally feel secure in the knowledge } that their contributions will be acknowledged properly, they will be } more inclined to help out, and may in time also more easily feel that } they can forgo co-authorships for what they consider "trivial" tasks. } This makes for a more healthy scientific environment. } } Best regards } Øystein } } - } Øystein Prytz, PhD | } Professor of Physics | Mobile: +47 93201512 } University of Oslo | Office: +47 22840684 } oystein.prytz-at-fys.uio.no | http://folk.uio.no/oysteinp/group } } } On 07.12.2020 21:35, xin-at-magnet.fsu.edu wrote: } } } } } } } } ---------------------------------------------------------------------------- } } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } } ---------------------------------------------------------------------------- } } } } Hi, All, } } } } I would appreciate if you can give me some input on standard practice } } concerning co-authorship on TEM data collection by the staff. } } Is it appropriate for the staff to ask for co-authorship if the TEM staff } } collects the TEM data and the TEM images are used in the publication? The } } TEM user only pays the machine time, not the staff labor time. There is } } no data analysis involved. } } Or in this case, the user should just acknowledge the staff with funding } } information in the acknowledgement? } } } } Thanks very much } } Yan Xin } } FSU } } } } =====End of - Headers==============================
==============================Original Headers============================== 10, 44 -- From greggps-at-umich.edu Tue Dec 8 14:29:40 2020 10, 44 -- Received: from mail-yb1-f169.google.com (mail-yb1-f169.google.com [209.85.219.169]) 10, 44 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0B8KTehg020955 10, 44 -- for {microscopy-at-microscopy.com} ; Tue, 8 Dec 2020 14:29:40 -0600 10, 44 -- Received: by mail-yb1-f169.google.com with SMTP id v67so4891728ybi.1 10, 44 -- for {microscopy-at-microscopy.com} ; Tue, 08 Dec 2020 12:43:48 -0800 (PST) 10, 44 -- DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 44 -- d=umich.edu; s=google-2016-06-03; 10, 44 -- h=mime-version:references:in-reply-to:from:date:message-id:subject:to 10, 44 -- :content-transfer-encoding; 10, 44 -- bh=KzFfLVSa2xSFjW1xezLX8LIlPT+oewq9arOmXv/fwhw=; 10, 44 -- b=ijUyIA6wylS5ep7aSD/4fITy9GA/VaeTqZrVig5sNUa0br4JicTvRh5caRLfSHy6e7 10, 44 -- siDsiG+gYkQ/6sH3RXPMpA4P8ULQq0a3xJ6grra8e59pUvABQu5Dhp4ZZ1+qR8FMbUDU 10, 44 -- jZrIxRmmtZ9xWEcJQjVNYYDGaHPK41q3YwPIPdabtyuKVYRQQakVQQhIFyAr4o4zhofn 10, 44 -- jbtPupU1QkZ8cNFyY0SVWD/JtjkQUqZjNo0FWCUGpmbYYuJbTZKPFIdAxISl3pGjg2Yu 10, 44 -- DMvzjxbMayXTGl3TpD5L0/eUDLrqY0rGapKFX+7Dpxo+mJvVM+jYQ0ovfCneFv+COPE2 10, 44 -- mOcQ== 10, 44 -- X-Google-DKIM-Signature: v=1; a=rsa-sha256; c=relaxed/relaxed; 10, 44 -- d=1e100.net; s=20161025; 10, 44 -- h=x-gm-message-state:mime-version:references:in-reply-to:from:date 10, 44 -- :message-id:subject:to:content-transfer-encoding; 10, 44 -- bh=KzFfLVSa2xSFjW1xezLX8LIlPT+oewq9arOmXv/fwhw=; 10, 44 -- b=RXMCqwu0/aZnZdoNph2k+SbbuYcXNaQ801fkawuWzCgGhd3Z+WDYPHgq85PrqeCAFe 10, 44 -- iLCiqub+538+JGm4+MIT9XTxzIwGCsPipcBWrBYEkiLo5/TeBfq1+QnmzV92jDCjuivI 10, 44 -- wo1Q69gWBx0n4QDzBbSLXVW6qOHYgD9MeR4vlsKC435OH0P8aVO94g4TKX1Yk0M0ceu6 10, 44 -- w/eg3H7Fz31KrHW+3qtsVnKfbD0fqFbw8kSA4WJj+YX45pF1fvw6z6il4OA2G11/p/Fq 10, 44 -- VLAzQ9jDSvBoQ8mXjX9mxyN0fYsBy2KDC5EFVnZ5l5/hfOnlLB1VUzG6pkbWXk1b7u5e 10, 44 -- aTIg== 10, 44 -- X-Gm-Message-State: AOAM532ipnlEgz7FQ6W9qHdpE/SNm/TOR7afPa5qRgvvD61LljnBiFx1 10, 44 -- 5wpL/syyk2BPhoXpDX7LUHHkvUM6wlW1Jj4Uac7tJoayR+w= 10, 44 -- X-Google-Smtp-Source: ABdhPJwcOQd6SCxKo12iaR3YHin4MZtFP+w1NCn5kc1PSjnkrQnXO6tFb6z6BZocQj1y0Up1ePmQgIH4/ndWHdMGGMs= 10, 44 -- X-Received: by 2002:a25:be89:: with SMTP id i9mr31094258ybk.35.1607460227892; 10, 44 -- Tue, 08 Dec 2020 12:43:47 -0800 (PST) 10, 44 -- MIME-Version: 1.0 10, 44 -- References: {202012080744.0B87irkU021566-at-microscopy.com} {CABg+575vSM_MtEZB=VZW-MJTL5Mi6PwbSCp52oeZWFTSdNLf=Q-at-mail.gmail.com} 10, 44 -- In-Reply-To: {CABg+575vSM_MtEZB=VZW-MJTL5Mi6PwbSCp52oeZWFTSdNLf=Q-at-mail.gmail.com} 10, 44 -- From: Gregg Sobocinski {greggps-at-umich.edu} 10, 44 -- Date: Tue, 8 Dec 2020 15:43:11 -0500 10, 44 -- Message-ID: {CABg+574GG7uzR+M+eWrxCt_cBZg52O-dA=fc0BP4dKpu3Rzr0g-at-mail.gmail.com} 10, 44 -- Subject: Fwd: [Microscopy] Re: inquiry on standard practice on co-authorship 10, 44 -- To: microscopy-at-microscopy.com 10, 44 -- Content-Type: text/plain; charset="UTF-8" 10, 44 -- Content-Transfer-Encoding: 8bit 10, 44 -- X-MIME-Autoconverted: from quoted-printable to 8bit by microscopy.com id 0B8KTehg020955 ==============================End of - Headers==============================
This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://microscopy.com/ --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both crya-at-loc.gov, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom ---------------------------------------------------------------------------
Email: crya-at-loc.gov Name: Cindy Connelly Ryan
Title-Subject: [Filtered] Fwd: Re: inquiry on standard practice on co-authorship
Message: I took a workshop earlier this year on exactly this topic - I suggest checking out the flowcharts and discussion documents on the COPE website (Committee on Publication Ethics). https://publicationethics.org/ Very helpful for working through who might only warrant an acknowledgement for a minor contribution and who deserves co-authorship. Also rather harsh words about ghost, guest, and courtesy "authors". So in the original poster's case, the question of whether that technician deserves a nod likely centers on whether they did the bulk of your benchwork for you, or just a little bit of it. (As a point of amusement - I am currently working on a review article summarizing how my lab's approach to one particular topic has evolved since our founding in 1971, and I have 49 years worth of possible people to credit! In many of those cases, posthumously. That will be a fun one to sort out.)
best,
Cindy Connelly Ryan Preservation Science Specialist Library of Congress Preservation Research and Testing Division Washington, D.C.
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Greetings, When journal articles got started (I am thinking late 1600's) and for a while after, authorship was easy. The author (usually just one) wrote the paper. Somewhere along the line the concept morphed from the writer to the doer, usually more than one. Complexity ensued. The guidelines that various societies have written up are helpful, if sometimes overly idealistic (for example, I think it is unrealistic to demand that person in lab X be "responsible" for results from lab Y in a paper that combines results from both). For me, I use the golden rule: If I had contributed to the project in such and such a way, and I was *not* given authorship, would I feel cheated? Admittedly subjective but works pretty well. Write on! Tobias Baskin Biology Department, UMass Amherst, USA
On 12/10/20 8:06 AM, microscopy.listserver-at-gmail.com wrote: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe --http://www.microscopy.com/MicroscopyListserver } On-Line Helphttp://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from:crya-at-loc.gov } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form athttp://microscopy.com/ } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } crya-at-loc.gov,Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit } from our collective wisdom } --------------------------------------------------------------------------- } } Email:crya-at-loc.gov Name: Cindy Connelly Ryan } } Title-Subject: [Filtered] Fwd: Re: inquiry on standard practice on co-authorship } } Message: I took a workshop earlier this year on exactly this topic - I suggest checking out the } flowcharts and discussion documents on the COPE website (Committee on Publication Ethics). } https://publicationethics.org/ } Very helpful for working through who might only warrant an acknowledgement for a minor contribution } and who deserves co-authorship. Also rather harsh words about ghost, guest, and courtesy "authors". } So in the original poster's case, the question of whether that technician deserves a nod likely } centers on whether they did the bulk of your benchwork for you, or just a little bit of it. } (As a point of amusement - I am currently working on a review article summarizing how my lab's } approach to one particular topic has evolved since our founding in 1971, and I have 49 years worth } of possible people to credit! In many of those cases, posthumously. That will be a fun one to sort } out.) } } best, } } Cindy Connelly Ryan } Preservation Science Specialist } Library of Congress Preservation Research and Testing Division } Washington, D.C. } } } Login Host: 140.147.94.239 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- }
Thank you, Cindy Connelly Ryan, for that reference.
It took me a bit to find the correct document, but it was appropriately succinct, describing my situations as "ghost author" and "gift author". Since department heads were specifically mentioned in the article, I'm guessing that my gift author experience was not unusual. Here is the shortcut, for those who wish to go right to it: https://publicationethics.org/files/2003pdf12_0.pdf
Regards, ~Gregg Gregg Sobocinski Microscope Imaging Suite, Managing Director University of Michigan, MCDB Dept. Ann Arbor, Michigan USA
On Thu, Dec 10, 2020 at 8:25 AM {microscopy.listserver-at-gmail.com} wrote: } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: crya-at-loc.gov } } This Question/Comment was submitted to the Microscopy Listserver } using the WWW based Form at http://microscopy.com/ } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } crya-at-loc.gov, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit } from our collective wisdom } --------------------------------------------------------------------------- } } Email: crya-at-loc.gov Name: Cindy Connelly Ryan } } Title-Subject: [Filtered] Fwd: Re: inquiry on standard practice on co-authorship } } Message: I took a workshop earlier this year on exactly this topic - I suggest checking out the } flowcharts and discussion documents on the COPE website (Committee on Publication Ethics). } https://publicationethics.org/ } Very helpful for working through who might only warrant an acknowledgement for a minor contribution } and who deserves co-authorship. Also rather harsh words about ghost, guest, and courtesy "authors". } So in the original poster's case, the question of whether that technician deserves a nod likely } centers on whether they did the bulk of your benchwork for you, or just a little bit of it. } (As a point of amusement - I am currently working on a review article summarizing how my lab's } approach to one particular topic has evolved since our founding in 1971, and I have 49 years worth } of possible people to credit! In many of those cases, posthumously. That will be a fun one to sort } out.) } } best, } } Cindy Connelly Ryan } Preservation Science Specialist } Library of Congress Preservation Research and Testing Division } Washington, D.C. } } } Login Host: 140.147.94.239 } Listserver Email Form V - 20120416 } --------------------------------------------------------------------------- } } ====End of - Headers==============================
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Email: andrew.sydor-at-sickkids.ca Name: Andrew Sydor
Title-Subject: [Filtered] Uranyl Acetate
Message: Hi everyone,
We are having a hard time finding uranyl acetate up here in Canada. Does anyone: 1) Know a Canadian supplier? 2) Know if this is a Canada-only problem or are other countries also experiencing this? 3) Know what the story is behind this issue (super curious)?
Thanks! -Andrew
Andrew Sydor, PhD Research Associate Brumell Lab, Cell Biology Program The Hospital for Sick Children Peter Gilgan Centre for Research and Learning 686 Bay Street, Rm. 19.9400 Bench N-S East Toronto ON M5G 0A4 P 416.813.6626
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Each lab and university obviously sets their own policy, but I do have some recommendations.
All facilities should require acknowledgments for use of their equipment, whether the user pays for just instrument time or a full service. This is a really easy argument to make. If the users are making use of your facility it means they need it, and will want it in the future and will want it to be excellent and well equipped. Acknowledgements make it easy to track the publication impact of usage and in doing so this invests in the future of the facility. So the argument you make to your users is "Do you want this facility to be here next year?", " Do you want us to be able to invest in equipment and staff that make your research easier?", "Do you want to avoid having to outsource experiments, and great inconvenience and cost?". An acknowledgement is not a large price to pay as a future investment!
The other argument you can make is that users do not pay the full cost and that their work is subsidised by the institution, and as such they need to acknowledge this. This is a negative argument and not as useful as the above. I have long been a fan of billing users the FULL costs of their microscope and staff time, and then adding a subsidy line to the bill so it is explicit who is actually paying.
Regarding co-authorship that is a grey area. As an academic (and a facility scientist) I am able to require co-authorship after a certain amount of work, usually if I spend more than a day on a users research (not training) this is, as far as I am concerned non-negotiable. But I appreciate that most facility staff are not in my position. As soon as staff have an intellectual investment in a project, beyond their day to day duties I feel this should be a co-authorship. Doing something new, development tasks anything that takes more time than the routine should be acknowledged with co-authorship. For small extra work a personalised mention in the acknowledgements is polite, but often this gets forgotten.
In your situation below I think the staff, if they are doing routine microscopy and this is part of their job, and not any special analysis, should not expect co-authorship. A personal acknowledgement would be polite, but a facility acknowledgement should be expected. The time factor is also an issue, if your staff are going above and beyond regarding the amount of time on one project, and in doing so offering intellectual input top a project this is a different matter.
Good luck,
Matthew
On 07.12.2020 21:35, xin-at-magnet.fsu.edu wrote: } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } Hi, All, } } I would appreciate if you can give me some input on standard practice } concerning co-authorship on TEM data collection by the staff. } Is it appropriate for the staff to ask for co-authorship if the TEM staff } collects the TEM data and the TEM images are used in the publication? The } TEM user only pays the machine time, not the staff labor time. There is } no data analysis involved. } Or in this case, the user should just acknowledge the staff with funding } information in the acknowledgement? } } Thanks very much } Yan Xin } FSU
Good morning Regulations on Uranyl Acetate have become very tough. It is not that there is a shortage of it we are making it and have it the question becomes does your facility allow for Depleted Uranium on site. We have ample supply to ship so please let us know how we can help to get you what you need. As well we offer and this may be of assistance to you and anyone else Uranyl replacements such as Uranyless and UAR . We are here to discuss the shipping of UA and any of the replacements s to you
Thank you and we look forward to hearing from you
Stacie Kirsch Electron Microscopy Sciences 1560 Industry Road Hatfield Pa 19440 E:Mail: stacie-at-ems-secure.com or info-at-emsdiasum.com Tel: 215-412-8400 Fax: 215-412-8450 www.emsdiasum.com
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Email: andrew.sydor-at-sickkids.ca {mailto:andrew.sydor-at-sickkids.ca} Name: Andrew Sydor
Title-Subject: [Filtered] Uranyl Acetate
Message: Hi everyone,
We are having a hard time finding uranyl acetate up here in Canada. Does anyone: 1) Know a Canadian supplier? 2) Know if this is a Canada-only problem or are other countries also experiencing this? 3) Know what the story is behind this issue (super curious)?
Thanks! -Andrew
Andrew Sydor, PhD Research Associate Brumell Lab, Cell Biology Program The Hospital for Sick Children Peter Gilgan Centre for Research and Learning 686 Bay Street, Rm. 19.9400 Bench N-S East Toronto ON M5G 0A4 P 416.813.6626
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The lab I was in at Harvard had heard that it was becoming more difficult to get UA in Canada. We don't know of any supplier in Canada as we purchase nearly everything from Electron Microscopy Sciences. Canada is likely going the route that Japan took in severely limiting the use of UA. There is a group at the University of Toronto you might reach out to that uses UA, at least for HPF-FS (ref - Mulcahy et al. Frontiers in Neural Circuits 2018).
You might want to consider alternatives such as gadolinium acetate and samarium acetate (refs: Nakakoshi et al. JEM 2011; Odriozola et al. bioRxiv 2017) which have shown promise particularly as /en bloc/ stains. You could also try UranyLess (from EMS - not sure about a Canadian supplier for this), but I have found it useful only for post-staining on grids, not for /en bloc/ work (if that's a concern).
Best,
Brandon
On Thu, Dec 10, 2020 at 6:52 PM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote:
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Email: andrew.sydor-at-sickkids.ca {mailto:andrew.sydor-at-sickkids.ca} Name: Andrew Sydor
Title-Subject: [Filtered] Uranyl Acetate
Message: Hi everyone,
We are having a hard time finding uranyl acetate up here in Canada. Does anyone: 1) Know a Canadian supplier? 2) Know if this is a Canada-only problem or are other countries also experiencing this? 3) Know what the story is behind this issue (super curious)?
Thanks! -Andrew
Andrew Sydor, PhD Research Associate Brumell Lab, Cell Biology Program The Hospital for Sick Children Peter Gilgan Centre for Research and Learning 686 Bay Street, Rm. 19.9400 Bench N-S East Toronto ON M5G 0A4 P 416.813.6626
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I want to image coronavirus particles sitting on dust particles by SEM. I intend to capture the dust particles on a filter for easy washing, dehydrate and sputter coat the filter.
I will start with a virus suspension so at some point I need to wash and dehydrate. I am not sure if virus particles withstand drying from water without fixation. What would you recommend me? Fixation? Any of dehydration process? Thank you in advance!
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Title-Subject: [Filtered] TEM - Oil is leaking for ODP pump
Message: Hi, I am using Tecnai12 Biotwin 120 kV TEM. The machine is not getting a vacuum in the projection chamber. I find out that oil is leaking from the ODP pump to the buffer tank.
Is anyone came across this issue? What makes an oil leak and how should I prevent it?
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Ravindra have you checked the sealing O-rings from the ODP pump? There must be 2 O-rings. One to the inlet point of the oil to the ODP and one to the draining point of the ODP. Sometimes, due to high temperatures, these O-rings are damaged and need replacement. Damaged O-rings can cause oil leaking. Panos
_________________________________________________________________ Dr. Panagiotis Berillis Associate Professor Microscopy and Image Analysis in Histology and in Aquatic Organisms Department of Ichthyology and Aquatic Environment School of Agricultural Sciences University of Thessaly Hellas (Greece) Tel: (+30)2421093248, (+30)6909711300
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, December 16, 2020 11:40 PM To: pveril-at-uth.gr
X-from: ravithakkar-at-ksu.edu
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Title-Subject: [Filtered] TEM - Oil is leaking for ODP pump
Message: Hi, I am using Tecnai12 Biotwin 120 kV TEM. The machine is not getting a vacuum in the projection chamber. I find out that oil is leaking from the ODP pump to the buffer tank.
Is anyone came across this issue? What makes an oil leak and how should I prevent it?
Login Host: 129.130.132.160 Listserver Email Form V - 20120416 ---------------------------------------------------------------------------
8 Heller Park Lane Somerset, NJ 08873 Telephone: 732-672-4840 E-mail: Jeff-at-ravescientific.com Web site: www.ravescientific.com {http://www.ravescientific.com}
---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html ---------------------------------------------------------------------------- X-from: Brandon Drescher {brandon.drescher-at-gmail.com} Hi Andrew, The lab I was in at Harvard had heard that it was becoming more difficult to get UA in Canada. We don't know of any supplier in Canada as we purchase nearly everything from Electron Microscopy Sciences. Canada is likely going the route that Japan took in severely limiting the use of UA. There is a group at the University of Toronto you might reach out to that uses UA, at least for HPF-FS (ref - Mulcahy et al. Frontiers in Neural Circuits 2018). You might want to consider alternatives such as gadolinium acetate and samarium acetate (refs: Nakakoshi et al. JEM 2011; Odriozola et al. bioRxiv 2017) which have shown promise particularly as /en bloc/ stains. You could also try UranyLess (from EMS - not sure about a Canadian supplier for this), but I have found it useful only for post-staining on grids, not for /en bloc/ work (if that's a concern). Best, Brandon On Thu, Dec 10, 2020 at 6:52 PM {microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} } wrote: ---------------------------------------------------------------------------- The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver On-Line {http://www.microscopy.com/MicroscopyListserverOn-Line} Help http://www.microscopy.com/MicroscopyListserver/FAQ.html {http://www.microscopy.com/MicroscopyListserver/FAQ.html} ---------------------------------------------------------------------------- X-from: andrew.sydor-at-sickkids.ca {mailto:andrew.sydor-at-sickkids.ca} This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at http://www.microscopy.com/MLFormMail.html {http://www.microscopy.com/MLFormMail.html} --------------------------------------------------------------------------- Remember this posting is most likely not from a Subscriber, so when replying please copy to both andrew.sydor-at-sickkids.ca {mailto:andrew.sydor-at-sickkids.ca} , Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can benefit from our collective wisdom --------------------------------------------------------------------------- Email: andrew.sydor-at-sickkids.ca {mailto:andrew.sydor-at-sickkids.ca} Name: Andrew Sydor Title-Subject: [Filtered] Uranyl Acetate Message: Hi everyone, We are having a hard time finding uranyl acetate up here in Canada. Does anyone: 1) Know a Canadian supplier? 2) Know if this is a Canada-only problem or are other countries also experiencing this? 3) Know what the story is behind this issue (super curious)? Thanks! -Andrew Andrew Sydor, PhD Research Associate Brumell Lab, Cell Biology Program The Hospital for Sick Children Peter Gilgan Centre for Research and Learning 686 Bay Street, Rm. 19.9400 Bench N-S East Toronto ON M5G 0A4 P 416.813.6626 Login Host: 192.75.158.166 Listserver Email Form V - 20120416 --------------------------------------------------------------------------- ==============================Original Headers============================== 10, 53 -- From microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} Thu Dec 10 17:31:47 2020 10, 53 -- Received: from mail-io1-f52.google.com {http://mail-io1-f52.google.com} (mail-io1-f52.google.com {http://mail-io1-f52.google.com} [209.85.166.52]) 10, 53 -- by microscopy.com {http://microscopy.com} (8.12.11.20060308/8.12.8) with ESMTP id 0BANVktO011352 10, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; Thu, 10 Dec 2020 17:31:47 -0600 10, 53 -- Received: by mail-io1-f52.google.com {http://mail-io1-f52.google.com} with SMTP id z5so7586572iob.11 10, 53 -- for {microscopy-at-microscopy.com {mailto:microscopy-at-microscopy.com} } ; 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if diffusion pump oil is spread to the buffer tank that mostly means that there is a big vacuum leak somewhere in the pumped volume of the TEM.
You have to search for leaks...
There should be a paragraph in the manual for this.
Best wishes,
Stefan
----------------------------------------------------- Stefan Diller - Scientific Photography Arndtstrasse 22 D - 97072 Wuerzburg Germany ++49-931-7848700 Phone ++49-931-7848701 Fax ++49-175-7177051 Mobile
Am 16.12.20 um 22:56 schrieb microscopy.listserver-at-gmail.com: } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America } To Subscribe/Unsubscribe -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: Panagiotis Berillis {pveril-at-apae.uth.gr} } } Ravindra have you checked the sealing O-rings from the ODP pump? There must be 2 O-rings. One to the } inlet point of the oil to the ODP and one to the draining point of the ODP. Sometimes, due to high } temperatures, these O-rings are damaged and need replacement. Damaged O-rings can cause oil leaking. } Panos } } _________________________________________________________________ } Dr. Panagiotis Berillis } Associate Professor Microscopy and Image Analysis in Histology and in Aquatic Organisms Department } of Ichthyology and Aquatic Environment } School of Agricultural Sciences University of Thessaly Hellas (Greece) } Tel: (+30)2421093248, (+30)6909711300 } } -----Original Message----- } X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com] Sent: Wednesday, } December 16, 2020 11:40 PM } To: pveril-at-uth.gr } Subject: [Microscopy] viaWWW: TEM - Oil is leaking for ODP pump } } } } } ---------------------------------------------------------------------------- } The Microscopy ListServer -- CoSponsor: The Microscopy Society of America To Subscribe/Unsubscribe } -- http://www.microscopy.com/MicroscopyListserver } On-Line Help http://www.microscopy.com/MicroscopyListserver/FAQ.html } ---------------------------------------------------------------------------- } } X-from: ravithakkar-at-ksu.edu } } This Question/Comment was submitted to the Microscopy Listserver using the WWW based Form at } http://microscopy.com/ } --------------------------------------------------------------------------- } Remember this posting is most likely not from a Subscriber, so when replying please copy to both } ravithakkar-at-ksu.edu, Microscopy-at-Microscopy.com so that all Microscopy Listserver Subscribers can } benefit from our collective wisdom } --------------------------------------------------------------------------- } } Email: ravithakkar-at-ksu.edu Name: Ravindra Thakkar } } Title-Subject: [Filtered] TEM - Oil is leaking for ODP pump } } Message: Hi, } I am using Tecnai12 Biotwin 120 kV TEM. The machine is not getting a vacuum in the projection } chamber. I find out that oil is leaking from the ODP pump to the buffer tank. } } Is anyone came across this issue? 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==============================Original Headers============================== 10, 29 -- From diller-at-stefan-diller.com Wed Dec 16 16:11:28 2020 10, 29 -- Received: from mailout022.rox.net (mailout022.rox.net [212.63.85.222]) 10, 29 -- by microscopy.com (8.12.11.20060308/8.12.8) with ESMTP id 0BGMBRe0032222 10, 29 -- for {microscopy-at-microscopy.com} ; Wed, 16 Dec 2020 16:11:28 -0600 10, 29 -- Received: from localhost ([127.0.0.1]) 10, 29 -- by mailout02.rox.net with esmtp (Exim 4.80) 10, 29 -- (envelope-from {diller-at-stefan-diller.com} ) 10, 29 -- id 1kpfFA-0006RQ-L3; Wed, 16 Dec 2020 23:26:00 +0100 10, 29 -- Received: from ip1f10fd5c.dynamic.kabel-deutschland.de ([31.16.253.92] helo=mac-pro.local) 10, 29 -- by mailout02.rox.net with esmtpsa (TLSv1.2:AES128-GCM-SHA256:128) 10, 29 -- (Exim 4.80) 10, 29 -- (envelope-from {diller-at-stefan-diller.com} ) 10, 29 -- id 1kpfFA-0006RH-Dy; Wed, 16 Dec 2020 23:26:00 +0100 10, 29 -- Subject: Re: [Microscopy] Fwd: viaWWW: TEM - Oil is leaking for ODP pump 10, 29 -- To: "Microscopy-at-microscopy.com" {microscopy-at-microscopy.com} , 10, 29 -- ravithakkar-at-ksu.edu 10, 29 -- References: {202012162156.0BGLuRk5021348-at-microscopy.com} 10, 29 -- From: stefan diller {diller-at-stefan-diller.com} 10, 29 -- Message-ID: {563aa276-c72e-0307-cf5a-ad4c1f84dc19-at-stefan-diller.com} 10, 29 -- Date: Wed, 16 Dec 2020 23:25:58 +0100 10, 29 -- User-Agent: Mozilla/5.0 (Macintosh; Intel Mac OS X 10.11; rv:78.0) 10, 29 -- Gecko/20100101 Thunderbird/78.5.1 10, 29 -- MIME-Version: 1.0 10, 29 -- In-Reply-To: {202012162156.0BGLuRk5021348-at-microscopy.com} 10, 29 -- Content-Type: text/plain; charset=windows-1252; format=flowed 10, 29 -- Content-Transfer-Encoding: 7bit 10, 29 -- Content-Language: de-DE 10, 29 -- X-Envelope-From: {diller-at-stefan-diller.com} 10, 29 -- X-Scanned-By: rockenstein AG ==============================End of - Headers==============================
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---------------------------------------------------------------------------------------------------- *From:* MIcroscopyListserver {microscopy.listserver-at-gmail.com} *Sent:* Wednesday, December 16, 2020 4:04 PM *To:* MicroscopyListServer-Forward {microscopy-at-microscopy.com} ; ravithakkar-at-ksu.edu {ravithakkar-at-ksu.edu} *Subject:* Fwd: [Microscopy] viaWWW: TEM - Oil is leaking for ODP pump X-from: Panagiotis Berillis {pveril-at-apae.uth.gr}
Ravindra have you checked the sealing O-rings from the ODP pump? There must be 2 O-rings. One to the inlet point of the oil to the ODP and one to the draining point of the ODP. Sometimes, due to high temperatures, these O-rings are damaged and need replacement. Damaged O-rings can cause oil leaking. Panos
_________________________________________________________________ Dr. Panagiotis Berillis Associate Professor Microscopy and Image Analysis in Histology and in Aquatic Organisms Department of Ichthyology and Aquatic Environment School of Agricultural Sciences University of Thessaly Hellas (Greece) Tel: (+30)2421093248, (+30)6909711300
-----Original Message----- X-from: microscopy.listserver-at-gmail.com [mailto:microscopy.listserver-at-gmail.com {mailto:microscopy.listserver-at-gmail.com} ] Sent: Wednesday, December 16, 2020 11:40 PM To: pveril-at-uth.gr
X-from: ravithakkar-at-ksu.edu
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Title-Subject: [Filtered] TEM - Oil is leaking for ODP pump
Message: Hi, I am using Tecnai12 Biotwin 120 kV TEM. The machine is not getting a vacuum in the projection chamber. I find out that oil is leaking from the ODP pump to the buffer tank.
Is anyone came across this issue? What makes an oil leak and how should I prevent it?
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X-from: Oshel, Philip Eugene {oshel1pe-at-cmich.edu}
Stephane,
I would suggest not dehydrating, etc. Freeze-fix - high-pressure freezing if you have access to that - followed by cryocoating with Pt or Ir and examing with a cryostage. If you can't cryocoat or don't have a cryostage, then freeze-dry (not in a lyophilizer) and coat with Pt or Ir. Not a regular sputter coater.
Phil ----------------------------------------- Philip Oshel Imaging Center Director Biology Department 1304 Biosciences 1455 Calumet Ct. Central Michigan University Mt. Pleasant, MI 48859 (989) 774-3576 office (989) 774-7567 lab
________________________________________ X-from: nizets2-at-yahoo.com {nizets2-at-yahoo.com} Sent: Wednesday, December 16, 2020 10:27 To: Oshel, Philip Eugene
Dear colleagues,
I want to image coronavirus particles sitting on dust particles by SEM. I intend to capture the dust particles on a filter for easy washing, dehydrate and sputter coat the filter.
I will start with a virus suspension so at some point I need to wash and dehydrate. I am not sure if virus particles withstand drying from water without fixation. What would you recommend me? Fixation? Any of dehydration process? Thank you in advance!