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From: Andreas Brech :      andreas.brech-at-bio.uio.no
Date: Thu, 28 Dec 1995 07:22:49 +0100
Subject: subscribe

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subscribe microscopy abrech-at-darwin.uio.no



Andreas Brech
Electron Microscopical Unit for Biological Sciences
Department of Biology, University of Oslo.
P.O.Box 1062 Blindern
N-0316 Oslo 3
Norway
Tel.: + 43-22 85 61 89 (work)
+ 43-22 43 83 23 (privat)
Fax.: + 43-22 85 47 26
e-mail.: abrech-at-bio.uio.no





From: wesleysm-at-biology.und.ac.za (James Wesley-Smith)
Date: Tue, 2 Jan 1996 08:52:00 +0200
Subject: Re: Texts for confocal and cryomicrotomy

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Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za}

Dear Fred

On the cryo side I recommend Patrick Echlin's
James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa





From: wesleysm-at-biology.und.ac.za (James Wesley-Smith)
Date: Tue, 2 Jan 1996 09:00:59 +0200
Subject: Re: Texts for confocal and cryomicrotomy

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Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za}

Ooops. Let's try again!
Dear Fred
On the cyo side I recommend Patick Echlin's Low Temperature
Microscopy and Analysis (Plenum Press) as an "all rounder"
reference textbook on the subject, including a chapter on cryomicrotomy.

Wish you and membres of this listeserver a prosperous and
aberration-free 1996.

James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa





From: hawkey-at-neuro.duke.edu (Larry Hawkey)
Date: Tue, 2 Jan 1996 09:15:29 -0500
Subject: advice needed on how to sell a TEM

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Dear Fellow Electron Microscopist

I am trying to sell our TEM. I have advertised in Microscopy Today and
Journal of the Microscopies and have had some interest, but I have not
gotten any bids. Our scope is five years old and in great condition. I
have heard that there is a flood of TEMs on the market, so the value of
TEMs has dropped. I would be interested in talking to anyone who has sold
or bought a scope resently. Duke has told us that we should take bids.
Would it be better for us if we set a price we could advertise?

Any help on this would be greatly appreciated.

Larry Hawkey
hawkey-at-neuro.duke.edu






From: Peter P Molnar MD PhD med./habil Debrecen :      MOLNARP-at-lib.dote.hu
Date: Tue, 2 Jan 1996 16:19:46 +100
Subject: rat karyotyping

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Happy New Year to Everybody! - Does anyone have a reference for
karyotyping rat cells? Any reference would be welcome!
Thanks in advance.
Peter P. Molnar, M.D., Ph.D.
molnarp-at-lib.dote.hu




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Tue, 2 Jan 1996 09:48:59 GMT
Subject: Archive

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For the past few months we have been archiving information from this mail
server, and other sources, that related to biological microscopy. There are
now 45 topics available. We hope that others can make good use of this
information.

go to: http://www.biotech.ufl.edu/~emcl. Then click the Wizard on the Tips
and Tricks block.

We will include any contributions that you might want to make and will
appreciate any comments that you might have.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: colijn-at-kcgl1.eng.ohio-state.edu (Henk Colijn)
Date: Tue, 02 Jan 1996 13:11:01 -0500 (EST)
Subject: Q: Polaroid negative clearing tanks

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Recently there was a thread about both commercial and home-made Polaroid
negative clearing tanks/racks. I apparently didn't keep a copy of the
thread. Could someone point me to the listserver archives or email me a
copy of the thread?

Thanks,

Henk Colijn

Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility
-------------------------------------------------------------------
An optimist believes that we live in the best of all possible worlds.
A pessimist fears this is true.






From: RNBALDUC-at-ARCRIDE.EDU.AR
Date: Tue, 2 Jan 1996 15:45 -0300
Subject: OIL IDENTIFICATION

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HY

I need know the caracteristics of the diffusion pump oil CIT - ALCATEL 220.

PLEASE, send the answer to "csedax-at-arcride.edu.ar"

thanks, in advance.

F. Balducci.




From: wise-at-vaxa.cis.uwosh.edu
Date: Tue, 02 Jan 1996 14:36:38 +0000
Subject: Flatbed scanners

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To all,

Although not strictly a microscopy question, perhaps someone has an
answer for me. We are considering buying a scanner for a Macintosh
computer. I have a catalog that lists them from $300 to $3000. Obviously,
there must be differences. Has anyone had any experience with scanners
(Relisys, Umax, Epson, Microtek)? Color vs B&W? Scan speed? DPI? How
about software? What's a good OCR program? Etc?

Bob


Robert R. Wise, PhD
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: Darrell.Wiens-at-uni.edu
Date: Tue, 02 Jan 1996 14:38:19 -0600 (CST)
Subject: join bulliten board

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subscribe Darrell.Wiens-at-uni.edu




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 2 Jan 1996 15:52:26 -0400
Subject: RE-Clearing Polaroid Negs

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Message-ID: {n1391524395.91458-at-mse.engin.umich.edu}

Subject: Time: 3:42 PM
OFFICE MEMO RE:Clearing Polaroid Negs Date: 1/2/96

For several years now three SEM labs here at the Univ of Michigan have not
been using sulfite baths for treating Poloroid P/N negatives. Instead, they
merely rinse them for an hour or so in warm running water (only a slow flow
is needed-just enough to keep the water lukish) and then hang them up by the
corner to drain and dry on spring-clip-type clothespins that are strung on a
piece of rope or wire. This eliminates the cost of the sulfite bath, the
problems of disposing of the spent bath liquor, and the ungodly mess that
students always produce by splashing the sulfite solution all over the lab.
Try it, you might find it satisfactory for your purposes. Wil Bigelow
(bigelow-at-umich.edu)





From: DENNIS WARD :      dw0005-at-epfl2.epflbalto.org
Date: Tue, 2 Jan 1996 18:45:07 -0500 (EST)
Subject: hair mitochondria

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Can anyone provide a few micrographs showing the gross
presence/distribution/morphology of mitochondria in the human
hair shaft? For teaching and demonstration purposes only.

Dennis Ward
dw0005-at-epfl2.epflbalto.org




From: f.lawrence-at-qut.edu.au (Felicity Lawrence)
Date: Wed, 03 Jan 1996 12:30:04 +1000
Subject: microscopical identification of minerals

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I am studying the microfractographic network and the mineral content of
bricks. In doing so it is my hope to use confocal microscopy to analyse the
microfractographical network of the bricks. However, I am not certain of the
common techniques used to identify the minerals that make up the bricks and
how to associate different mineral grains to the fissures that may traverse
them. It is my hope that I can develope a multi-digital image analysis of
the bricks' fracture network and its mineral composition. For mineral
identification I have found a number of techniques discussed in the
literature such as polarised light microscopy, acoustic microscopy, SEM
(Backscattered electron imaging and energy diepersive X-Ray mapping) and
uranium -induced fission tracked micro-mapping. I would like to request any
information on these techniques or any such others that may be used in
conjunction with CLSM for mineral identification of the composition of clay
bricks as to form a multi-image analysis technique.

Thank-you,

Felicity Lawrence
e-mail : f.lawrence-at-qut.edu.au





From: Robert McDonald :      robert-at-geology.gla.ac.uk
Date: Wed, 3 Jan 1996 08:39:58 GMT
Subject: microscopical identification of minerals

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Message-Id: {9912.199601030839-at-starav.}


subscribe microscopy robert-at-geology.gla.ac.uk




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Wed, 3 Jan 1996 08:58:33 GMT
Subject: Re: Flatbed scanners

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} To all,
}
} Although not strictly a microscopy question, perhaps someone has an
} answer for me. We are considering buying a scanner for a Macintosh
} computer. I have a catalog that lists them from $300 to $3000. Obviously,
} there must be differences. Has anyone had any experience with scanners
} (Relisys, Umax, Epson, Microtek)? Color vs B&W? Scan speed? DPI? How
} about software? What's a good OCR program? Etc?
}
} Bob
}
}
} Robert R. Wise, PhD
} Department of Biology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} (414) 424-3404 tel
} (414) 424-1101 fax
} wise-at-vaxa.cis.uwosh.edu
}
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
We are running a Umax Power-look with a transparency scanner for doing
negatives. It come with all the right software including Adobe Photoshop
for either PC or MAC. I think it was about $2600. directly from Umax. We
have been very happy with it so far. Another lab has the same scanner
running on an SGI Indy. Apparently the software is more tricky on that
platform but the results have been very good.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Chris Frethem (CBN) :      frethem-at-lenti.med.umn.edu
Date: Wed, 3 Jan 1996 11:45:52 -0600 (CST)
Subject: Subscribe

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Message-Id: {m0tXVRp-0005UzC-at-island.amtsgi.bc.ca}


subscribe frethem-at-lenti.med.umn.edu



=======================================================================
Chris Frethem (612)624-4652 (voice)
Cell Biology & Neuroanatomy (612)624-8118 (FAX)
U of MN, Minneapolis e-mail: frethem-at-lenti.med.umn.edu







From: wschweitzer-at-access.ch (Wolf Schweitzer)
Date: Wed, 3 Jan 1996 18:11:02 +0100
Subject: Re: Flatbed scanners

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X-Sender: wschweitzer-at-mail.access.ch
Message-Id: {v01510100ad1067b05190-at-[193.246.40.108]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I am using a Hewlett Packard ScanJett IIc, about 800 dpi with some
interpolation,
color / bw. This is an "older" model, so I bought a used one for 500 sFr.
(swiss franks).

- OCR even for small printed text (as in journals) is satisfying with OmniPage.
- Microscopic slides *can* be scanned, resulting in about a 30 fold
magnification.

Personally I am very satisfied with this device. Good Luck, Wolf.



} To all,
}
} Although not strictly a microscopy question, perhaps someone has an
} answer for me. We are considering buying a scanner for a Macintosh
} computer. I have a catalog that lists them from $300 to $3000. Obviously,
} there must be differences. Has anyone had any experience with scanners
} (Relisys, Umax, Epson, Microtek)? Color vs B&W? Scan speed? DPI? How
} about software? What's a good OCR program? Etc?
}
} Bob
}
}
} Robert R. Wise, PhD
} Department of Biology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} (414) 424-3404 tel
} (414) 424-1101 fax
} wise-at-vaxa.cis.uwosh.edu


------------------------------------------------------------------
W.Schweitzer, MD / CH-8596 Scherzingen/TG / wschweitzer-at-access.ch
Tel+Fax 077 877203/URL:"http://www.access.ch/whoiswho/wschweitzer"






From: hallel :      hallel-at-macgw1.crd.ge.com
Date: 3 Jan 1996 09:22:50 U
Subject: MSA Call For Nominations

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Message-Id: {n1391461579.82389-at-Macgw1.crd.ge.com}

MSA is seeking nominations for its major annual awards, to be presented at the
1996 Annual Meeting in Minneapolis, MN in August. Nominations must be
received by January 15, 1996, and should consist of a brief description of why
the nominee is deserving of the award and, if possible, a CV or resume.
Additional notes of support from colleagues are also helpful.

The major MSA awards are as follows:

MSA Distinguished Scientist Awards (Physical and Biological): given for
unique and distinguished contributions to microscopy, imaging, and/or
compositional analysis.

Burton Medal: given for the most important contribution(s) in the fields of
microscopy, imaging, and/or compositional analysis during the last five years
by a person under the age of 35 on June 30, 1996. Preference will be given
for work carried out in North America.

Morton D Maser Distinguished Service Award: given to honor significant
volunteer service to MSA over a period of years and generally in more than one
area.

Outstanding Technologist Award: given to honor outstanding contributions by a
technologist.

Nominations or questions can be addressed to:

Ernie Hall
MSA Physical Sciences Director
GE CRD, PO Box 8 (1 Research Circle), Schenectady, NY 12301
e-mail: hallel-at-crd.ge.com
fax: 518-387-6972
phone: 518-387-6677

Conly Rieder
MSA Biological Sciences Director
Wadsworth Center, Emipre State Plaza, PO Box 509, Albany, NY 12201
e-mail: rieder-at-wadsworth.org
fax: 518-486-4901
phone: 518-474-6774




From: Ilene Sugino :      suginoik-at-UMDNJ.EDU
Date: Wed, 3 Jan 1996 12:23:05 -0500 (EST)
Subject: job openings

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Message-Id: {199601031723.LAA03657-at-Sparc5.Microscopy.Com}

The Ocular Cell Transplantation Laboratory in the Department of
Ophthalmology at UMDNJ, New Jersey Medical School has the following job
openings:

ELECTRON MICROSCOPIST: The individual will be expected to perform all
aspects of TEM from harvesting animal tissue to preparation of
publication prints. In addition, some specialized EM techniques will be
performed such as immunocytochemistry and in situ hybridization. Animal
work is required and will involve assisting in surgeries and euthanasia.
We are seeking a skilled electron microscopist with a minimum of three
years experience after receiving a bachelor's degree.

RESEARCH TEACHING SPECIALIST: This individual will be expected to
harvest tissue and prepare it for light and electron microscopy.
Embedding media will mainly involve epon, JB4, or lowicryl. Animal work
is required. A bachelor's degree plus one year of relevant experience
after receipt of the degree are the minimum requirements for this
position. Individual must have experience embedding and sectioning
tissue embedded in epon and JB4. Experience with black and white
photography highly desirable.

MICROSCOPIST: Medjet, Inc., a biotech company specializing in the
development of ophthalmic instruments is seeking a skilled microscopist
to conduct animal studies. This position requires an individual with
skills in both TEM and SEM. The individual will be expected to work
independently and prepare and present data at weekly meetings. The
position requires commuting between UMDNJ in Newark to the Medjet
facilities in Edison, NJ (about an hour drive). A PHD or MA is required
with a minimum of 3 years relevant experience.

Interested individuals should mail or fax their resumes to:--



------------------------------------------------------------------------------

Ilene Sugino e-mail: suginoik-at-umdnj.edu
UMDNJ-Ophthalmology
DOC 6th Floor fax: (201) 982-7762
90 Bergen Street
Newark, New Jersey 07103

------------------------------------------------------------------------------




From: huffe-at-pgL7.chem.nyu.edu (Edward J. Huff)
Date: Wed, 3 Jan 1996 15:44:56 -0500
Subject: How are coverslips made?

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Does anyone have a reference to a description of the
actual method of making coverslips? Is it true that
they are float glass? Wouldn't that mean that one side
might be different from another (not optically, but maybe
chemically)? Inquiring minds need to know...




From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Wed, 3 Jan 1996 12:11:54 -0500
Subject: Re: Flatbed scanners

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Message-Id: {v01520d00ad104b6e3c75-at-[155.37.2.10]}
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{ We are considering buying a scanner}


Bob, we are using extensively flatbed scanners for digital imaging of our
negatives (fluorescence and histology color LM, TEM and old Polaroids from
my FSEM). My suggestion: Buy an Agfa Arcus II for true 8-12 bit graytone
or 24-48 bit color "output" in TIFF. AGFA is experienced, has excellent
color handling and will be around for a long time for support. The street
price of US$ 2,000 (any US catalog company, i.e., The Mac/PC Zone
1-800-248-0800) includes a "full version" of Photoshop 3.04 which reads and
writes 8-12 bit Tif files. TIFF is the MSA standard. Both versions (PC and
Mac) of Photoshop 3.04 are now available and allow us to work with 12-bit
Tiff's (at my microscope on a PC and at my networked office with a Mac).
The scanner comes with AGFA's FOTOLOOK acquisition software which is opened
from within Photoshop: Files-Acquisition.

Please consider: Not spatial resolution but "contrast resolution" is
important for the acquisition of digital image data, i.e., if you read more
intensity steps you can work with more intensity levels per pixels and can
"see" smaller contrasts. Our experience is that all microscopes can deliver
12-14 bit contrast resolution. The jump from 8-bit to 12-bit gives you an
increase in contrast resolution of 1:16 which is adequate for utilizing the
full range of contrasts found in (TEM, Polaroid) negatives. Their is
already some software out for the handling of 12-bit image data and I
believe that soon many other software packages will available for working
at the level of 12-bit contrast resolution already available in many
microscopes, e.g. AFM microscopes, good CCD cameras for TEM and several SEM
acquisition systems. Even, if you cannot handle 12-bit data at this time
and may have to wait for an upgrade of NIH image, a foreseeing investment
in adequate hardware will pay of very soon when 12-bit contrast resolution
will have a common place in microscopy imaging.

Optimal scanning of negatives requires that the negatives are exposed 1-2
stops more than for ordinary photographic printing (high lights should be
"covered" and not be empty, dark areas should be very dark. Good scanners
can handle more than 3.0 optical density which is "very dense" for a
photographic negative). Also the scanning procedures must be adapted in
order to take advantage of the full contrast transfer function of the
negatives as well as the scanner. I am in the process of putting a report
together at my WWW site on our experience with 8-bit versus 12-bit negative
scanning.

Best regards Klaus

******************************************************************************
* : *
* Klaus-Ruediger Peters, Ph.D. : WWW Home Page: *
* Director, Molecular Imaging Laboratgory : *
* Biomolecular Structure Analysis Center : Molecular Imaging Laboratory *
* University of Connecticut Health Center : http://panda.uchc.edu/ *
* 263 Farmington Ave. : htklaus/index.html *
* Farmington, CT 06030-2017; U.S.A : Differential Hysteresis *
* : Processing Demo at http:// *
* Tel: (203) 679-3977; Fax: (203) 679-1989 : panda.uchc.edu./htbit/indiv/ *
* e-mail: Peters-at-BSAC.UCHC.EDU : /software_docs/dhp.html *
* : *
****************************************************************************
**






From: Greg :      greg-at-umic.umic.sunysb.edu
Date: Wed, 3 Jan 1996 16:55:32 EST5DST
Subject: TEM of yeast

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I am looking for a protocol for the embedding of yeast for
TEM. I made a few attempts and the results have been
mixed. The biggest problem is that the cut sections look
like Swiss cheese. Where yeast should be there are holes.
The fixation appears to be fine in the yeast that do not
fall out. I am treating the cells with KMnO4.
Over weekend infiltrations with Spurr's or Epon 812 have
not worked. Any suggestions would be helpful.

Thanks, Greg Rudomen
Greg-at-umic.umic.sunysb.edu





From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 3 Jan 1996 12:06:54 -0500 (EST)
Subject: Re: Flatbed scanners

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} We are running a Umax Power-look with a transparency scanner for doing
} negatives. It come with all the right software including Adobe Photoshop
} for either PC or MAC. I think it was about $2600. directly from Umax. We
} have been very happy with it so far. Another lab has the same scanner
} running on an SGI Indy. Apparently the software is more tricky on that
} platform but the results have been very good.

Dear Greg,
What is the resolution of this scanner? How accurate is the quan-
titation of the OD's? Would it be a suitable substitute for the Perkin-
Elmer flatbed microdensitometer? TIA.
Yours,
Bill Tivol




From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 3 Jan 1996 15:50:15 -0500
Subject: Gray FB scan

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Message-Id: {n1391437919.10686-at-msmail.tmc.tulane.edu}

Macintosh system

If black and white prints are to be scanned, the Apple one Scanner (up to 1200
dpi) cost now less than 700 dollars and is very good with OFOTO software.
Equivalent scanners (e.g. LaCie are just a good), but I am not sure about PC
equivalent and would certainly like to know the outcome of recommendations.
Images in the page below were scanned with the Apple one scanner at 300dpifrom
black and white prints without any manipulation (filtering, etc).


******************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} Prof. Pathology & Otolaryngology *
* http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html *
******************************************************************

Felices Pascuas!






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Wed, 3 Jan 1996 19:43:47 -0600
Subject: Re: TEM of yeast

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} I am looking for a protocol for the embedding of yeast for
} TEM. I made a few attempts and the results have been
} mixed. The biggest problem is that the cut sections look
} like Swiss cheese. Where yeast should be there are holes.
} The fixation appears to be fine in the yeast that do not
} fall out. I am treating the cells with KMnO4.
} Over weekend infiltrations with Spurr's or Epon 812 have
} not worked. Any suggestions would be helpful.
}
} Thanks, Greg Rudomen
} Greg-at-umic.umic.sunysb.edu

RESPONSE:

Hi Greg,
I've worked with Candida albicans and Saccharomyces over the years and
rarely had problems using KMnO4 fixation and embedding in either Spurr's or
Epon-type resins. Check that the cells are completely dehydrated (is the
alcohol or acetone really absolute?) and that there is no water in the
resins.

I have used the following protocol:
After fixation, rinse 3x in distilled water 20 min ea. If individual cells,
suspend the cells in 2% agarose at 45 C, pour onto glass slide and allow to
solidify. Cut into small cubes. Suspend briefly in dist water and then
dehydrate in ethanol series (25 min ea), 25, 50, 75, 95, ABS x 3 changes.
Prop oxide 3 x 25 min. I use a l:l mix of Epon/Spurr's resin made up for
long pot life. Infiltrate as follows: 2 PO/1 resin for 1 day, equal parts
PO/resin for 1 day. Pure resin in capped container at RT 3 changes at 1 day
each. Transfer specimens into capsules and polymerize at 60 C for 48 hr.

CALL IF YOU NEED MORE INFO.




#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: albrite-at-netcom.com (larry)
Date: Wed, 3 Jan 1996 20:13:32 -0800
Subject: WTB Iris Diaphragm

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Message-Id: {199601040305.AA16027-at-ux1.cso.uiuc.edu}

I am looking for an iris diaphragm for a Nikon SMZ-10 Stereo microscope.
They have discontinued this item, it is a double diaphragm wafer that goes
in the microscope stack. The Part number is 76270. I am also looking for
a fine focus attachment for the Nikon camera UFX. It fits over the eyepiece
of the camera.
Larry Albright
419 Sunset Avenue
Venice, California 90291
Phone 310-399-0865...Fax 310-392-9222 or albrite-at-netcom.com




From: wesleysm-at-biology.und.ac.za (James Wesley-Smith)
Date: Thu, 4 Jan 1996 12:41:20 +0200
Subject: Re: TEM of yeast

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Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za}

Dear Greg
You may also want to try freeze-substitution. Although it sounds
like more than you bargained for, it works superbly for yeast
(personal experience). Because of their small size, they freeze and
substitute well, and the preservation attained is worth the effort.

Try and get hold of the article by Baba and Osumi in the Journal
of Electron Microscopy Technique, March 1987, Vol 5, number 3, p249.

Good luck.

James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa





From: sco.umc2-at-Mail.health.ufl.edu
Date: Thu, 04 Jan 1996 05:02:20 -0500
Subject: Flatbed Scanners

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One user's opinions:
1. Consider the media you will be scanning. Some scanners do
better at transparencies than others (nikon has a slide scanner
for 3K$), some are designed for large format (Umax has a 17 x 12
inch for 7k$), some do black and white on the cheap (HP has one
B&W for $400).

2. Consider the destination of the image. There is no use
buying a 30 bit high definition scanner if all you will do is
newsprint. A 300dpi 256 gray scanner goes a long way with laser
printers and costs $400.

3. Consider the resolution you need. If you are going to scan
stamps that are 1 inch, and display them at poster size, you need
a very high res scanner (1200 dpi or more) if you are going to
scan 8 x 10 positives and display them in a small window, then
600 is plenty, 300 adequate.

4. Consider the speed. Some scanners take 30 seconds to scan
one page. This can be a real pain if you have any number of
images to scan. Some are available with a document feeder, which
is a nice thing to have, but not if all your scans will be of
pictures (which must be hand fed anyway)
"Single pass" scanning means that all 3 colors (red green blue)
are captured simultaneously--this is faster (HP scanjet 4 offers
this)

5. Real Res. Most scanners interpolate the dpi at high res.
This is ok, but non-interpolated scans are better. Be willing to
pay more for a hardware 600dpi than a software 600dpi.





From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Thu, 04 Jan 1996 15:53:50 +0000
Subject: Cell substrates for TEM sections

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Message-Id: {s0ebf7db.028-at-wpo.nerc.ac.uk}
X-Mailer: Novell GroupWise 4.1

Does anyone have any experience or advice on substrates suitable for
ultramicrotomy of settled organisms for TEM?

We have someone culturing small marine sea-squirts (Ascideans) who
wants to cut ultrathin sections. These form spreading colonies about 1 mm
in height. I have seen references somewhere to Mercox/Mercanox? but do
not know its source/supplier. Also can this be cut on glass knives - marine
organisms are notorious harbourers of small sand particles which do not go
well with diamond knives!

Any comments welcome.

Keith Ryan
Plymouth Marine Laboratory
Citadel Hill
Plymouth PL1 2PB, UK

e-mail: k.ryan-at-pml.ac.uk





From: Darrell.Wiens-at-uni.edu
Date: Thu, 04 Jan 1996 10:55:23 -0600 (CST)
Subject: LM Microtome shopping

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I am prepared to purchase a microtome for LM. I have info on the Zeiss HM 315
and Zeiss HM 325 (the one with specimen retraction. I also have info on the
Leica Histocut 820. I wish to section chick embryos from very early stages, in
paraffin blocks for immunostaining purposes. I have been using a very old AO
Spencer. I am interested in "bang for the buck" value. Is the specimen
retraction feature worthwhile for paraffin block sectioning? Are the
disposable blades as stable as the stainless steel knives or can one get
sectioning artifacts when using very small block faces? Any advice or
suggestions?
Darrell Wiens
University of Northern Iowa




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 4 Jan 1996 09:30:48 GMT
Subject: Re: TEM of yeast

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} I am looking for a protocol for the embedding of yeast for
} TEM. I made a few attempts and the results have been
} mixed. The biggest problem is that the cut sections look
} like Swiss cheese. Where yeast should be there are holes.
} The fixation appears to be fine in the yeast that do not
} fall out. I am treating the cells with KMnO4.
} Over weekend infiltrations with Spurr's or Epon 812 have
} not worked. Any suggestions would be helpful.
}
} Thanks, Greg Rudomen
} Greg-at-umic.umic.sunysb.edu
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

Greg,
See: Anderson et al., 1991 J EM Tech. 18:172 for a protocol using
periodate treatment of the wall.

and, Byers & Goetsch, 1991 Methods in Enzymology 194:602 for a
method using enzymatic treatment of the wall.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 4 Jan 1996 09:15:39 GMT
Subject: Re: Flatbed scanners

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K.-R. Peters wrote:

} Optimal scanning of negatives requires that the negatives are exposed 1-2
} stops more than for ordinary photographic printing (high lights should be
} "covered" and not be empty, dark areas should be very dark. Good scanners
} can handle more than 3.0 optical density which is "very dense" for a
} photographic negative). Also the scanning procedures must be adapted in
} order to take advantage of the full contrast transfer function of the
} negatives as well as the scanner. I am in the process of putting a report
} together at my WWW site on our experience with 8-bit versus 12-bit negative
} scanning.
}
} Best regards Klaus
}
} ******************************************************************************
} * : *
} * Klaus-Ruediger Peters, Ph.D. : WWW Home Page: *
} * Director, Molecular Imaging Laboratgory : *
} * Biomolecular Structure Analysis Center : Molecular Imaging Laboratory *
} * University of Connecticut Health Center : http://panda.uchc.edu/ *
} * 263 Farmington Ave. : htklaus/index.html *
} * Farmington, CT 06030-2017; U.S.A : Differential Hysteresis *
} * : Processing Demo at http:// *
} * Tel: (203) 679-3977; Fax: (203) 679-1989 : panda.uchc.edu./htbit/indiv/ *
} * e-mail: Peters-at-BSAC.UCHC.EDU : /software_docs/dhp.html *
} * : *
} ****************************************************************************
} **
}
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
we have partially overcome this problem by overlaying negatives with
photographic contrast filters or neutral density filters. This may not be
appropriate for very high res. work but is no problem for video display and
printing to a laser printer
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: wise-at-vaxa.cis.uwosh.edu
Date: Thu, 04 Jan 1996 14:07:07 +0000
Subject: "Antique" microscopes

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Our department has about a dozen old Spencer light microscopes--monoccular,
black laquer with lots of brass, probably pre-WWII. Does anyone know if
there is a market for such things out there? Are there 'antique'
microscope dealers who might be interested in these? They are not very
functional but too nice to throw away.

Bob


Robert R. Wise, PhD
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: jbpawley-at-facstaff.wisc.edu (Jim Pawley)
Date: Thu, 4 Jan 1996 12:13:15 -0600
Subject: Re: Flatbed scanners

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In response to Klaus Peter's eloquent plea for 12-bit versus 8-bit scanners:

While I would not want to be thought of against precision, it must be
admitted that there are some limitations to using 12-bit data, namely
greater storage (and display?) requirements. So one should consider this
step carefully.

Assuming that it is done correctly, 12-bit data is digitized to 1 part in
4,000 while 8-bit data is digitized to only 1 part in 256. Therefore, to
make proper use of such precision one must have data of similar precision.
Although many considerations can reduce the precision of recorded data:
measurement noise, low contrast, it can never be better than the
statistical limitations placed by quantum mechanics on the number of events
counted (grains of silver (assuming that they are all of the same size
which whey aren't)/pixel or photons/pixel or electrons/pixel). Clearly,
this all depends on the size of a pixel: larger pixels imply the chance to
count more quanta (but they also mean lower scanner resolution) so you may
need less intensity resolution if you have greater real (non interpolated)
spatial resolution.

Now it is clear that, for instance, in STM, where a 0.05 nm height
resolution can be detected and the piezo may have a height sensing range of
many microns, there is no difficulty in obtaining 12-bit data (or even
16-bit data) although you may have trouble displaying all of this "depth"
to a human observer at one time. Likewise, CCD sensors having noise levels
of +/- 3-4 RMS electrons/pixel and full-well signal levels of 300,000
electrons/pixel easily satisfy the 4,000:1 (more like 100,000:1 possible)
requirement as long as you use a long enough exposure to actually record at
least 20,000 electrons/pixel. However, in confocal microscopy, 256
photons/pixel is often quite a high signal and digitizing from a color
slide of a fluorescent image recorded on high speed film (depending on
original magnification and pixel size. Remember, 1200 DPI implies 20 micron
pixels.) one may find it difficult to find even 10 distinguishable levels.

So far we have only spoken of linear digitization (as is appropriate to
scanners which usually make every effort to be linear). However, for
signals degraded mainly by statistical noise and recorded by the direct
digitization of electron signals, the separation between "meaningful" grey
levels (those separated in intensity from neighboring levels by an amount
at least equal to their standard deviation?) the difference between the
first two such levels (1 event/pixel and 4 events/pixel) is 3 event/pixel
whereas that between the 15th level and the 16th is 31 events/pixel. In
other words, if we are only interested in recording the INFORMATION in a
signal limited only by quantum noise (SEM? Confocal?), we could first take
its square root and then digitize this. This could be done using only one
half of the number of bits that would have been necessary to preserve the
information in a linear signal and each SQRTBit would be a "real" grey
level.

All this said, one must admit that getting real 8-bit data requires more
than the use of an 8-bit DAC (0.4% illumination stability, low-noise
detector, freedom from digital electronic interference, proper bandwidth
for sampling rate in both the electron and optical parts of the information
path, etc) and many scanners may not provide the performance they claim.
This might explain much of the difference claimed to exist between 8-bit
and 12-bit results.

(To any owners who have read this far: have you any MEASUREMENTS on scanner
performance on known images?)

SEM might provide a good case in point: The SE signal variation associated
with small features is often less than 1% of the total signal. As "seeing"
a small feature with only 1% contrast requires collecting at least 1/0.01
x0.01 = 10,000 electrons/pixel, it might seem worthwhile to use 12-bits to
preserve these small variations. However, unless there are "holes" in your
specimen from which virtually no signal is obtained (i.e. if your signal
does not have "important" high contrast), little would be lost if one
digitized only the 256 levels nearest in intensity to those of the small
feature and a positive improvement would be gained (all the information
that could possibly be coded in up to 65,000 detected electrons vs. 10,000)
by digitizing the square-root of the signal into only 8 bits. In either
case you would have to "process" the signal before you displayed it on the
screen.

Just some thoughts. Sorry that they were so long but I felt a trend to
Digital-One-up-man-ship...

Jim Pawley

****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU






From: Joyce Palmer, ECE Marcus 20 413-545-4647 :      PALMER-at-ecs.umass.edu
Date: Thu, 04 Jan 1996 15:51:40 -0500
Subject: ball and stick models of crystals

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Dear Colleages,
I'm having trouble locating a simple item - those plastic ball and
stick models for making models of simple crystals. I need some models for a
course I'm teaching in the spring, and so far the models I've seen in the
scientific/lab products catalogs I've looked in are a bit disapointing. By
that, I mean they are more oriented toward molecular models, and the emphasis
in the models are on the bonds, not the arrangement of the atoms, which is
what I'd rather stress for this class (intro to semiconductors).
Does anyone out there know of any good sources for ball and stick
models?
Thanks

Joyce Palmer
Umass Amherst ECE department





From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 4 Jan 1996 17:00:38 -0500 (EST)
Subject: Re: TEM of yeast

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}
} I am looking for a protocol for the embedding of yeast for
} TEM. I made a few attempts and the results have been
} mixed. The biggest problem is that the cut sections look
} like Swiss cheese. Where yeast should be there are holes.

Dear Greg,
I had the same problem with pollen grains, and it arises from the
difference in hardness between the specimen and the resin. Our solution
was to experiment with different proportions of the constituents of the
resin until its hardness matched that of the specimen. Of course, this has
to be a trial-and-error process. Good luck.
Yours,
Bill Tivol




From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Thu, 4 Jan 1996 15:20:41 -0500
Subject: Re: Flatbed scanners: 12-bit versus 8-bit

Contents Retrieved from Microscopy Listserver Archives
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} } K.-R. Peters wrote:
} }
} } Optimal scanning of negatives requires that the negatives are exposed 1-2
} } stops more than for ordinary photographic printing (high lights should be
} } "covered" and not be empty, dark areas should be very dark. Good scanners


} Greg Erdos responded:
}
} we have partially overcome this problem by overlaying negatives with
} photographic contrast filters or neutral density filters. This may not be
} appropriate for very high res. work but is no problem for video display and
} printing to a laser printer
} -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
} Greg Erdos Phone: 352-392-1295
} Scientific Director,
} ICBR Electron Microscopy Core Lab
} 218 Carr Hall Fax: 352-846-0251
} University of Florida E-mail: gwe-at-biotech.ufl.edu
} Gainesville, FL 32611
} -at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-


Thanks Greg, you make an important point concerning acquisition and display:

The application of contrast or neutral filters in photography shifts the
brightness (level) or contrast (slope) of the image data to a range at
which the eye is more perceptive (details in high lights may be preserved,
but reduced in the shadows or visa versa, or both may be compressed). These
procedures do not produce more "image detail" than the negative contains
but modifies the display of the details. If during image acquisition
(negative exposure) the highlights in the negatives were underexposed (or
the shadows overexposed) then the possibly lost detail information can not
be recovered, neither analog nor digitally.

It is therefore important (for both analog as well as digital imaging of
the negative) to the set the brightness level of the negative so that it
captures as much information as possible. Generally, the contrast transfer
function (slope) is not changed, i.e., through variation of developing
conditions or change of film/developing combination. But why don't we
normally care much about optimal exposures (or acquisition)?

Photography (analog) had long accepted that there is more information in a
negative (raw data) than the eye can "see" ("image" being that part of the
displayed image data which the eye can perceive and recognize). It
developed many useful tools for enhancing the contrast of certain image
components of the negative so that they become visible, but consider our
following findings. Measurments of the intensity ranges of contrast
patterns in digitized EM negatives and the contrast ranges required for
visual display of these contrast patterns (as images) indicates that an
optimally exposed TEM negative has an information depth of approximately 12
bit, or a 12-bit contrast resolution whereas the "image" has merely an
approximate 6-bit contrast resolution. We find in digital image data from
nearly all microscopies that the detail contrasts (structures of lowest
contrast levels) occupy only 1-10 % of the overall contrast range of the
image data whereas we need about 25% of the contrast range of an image for
visual recognition (not perception) of a contrast pattern (in an amorphous
environment). This means, most of the low-contrast data components (which
constitute the high-resolution details) are not visible in a conventional
image of the data. Unfortunately, we have adapted to this fact and tend to
acquire images at an insufficiently low contrast resolution level equal to
that of our visual system because we are not missing anything in the
"empty" high-lights or empty shadows although our microscopes can acquire
images at much higher contrast resolution. On the other hand, these facts
underline why digital acquisition (from the imaging sensor or from the
negative) should be done at a 12-bit level instead of an conventional 8-bit
level. Many manufactures of acquisition and processing equipment start to
provide a 12-bit "output" level, 12-bit scanners are now offered at catalog
firms, and Photoshop is now available for PCs and Macs with 12-bit TIFF
capabilities (version 3.04).

The basic idea of scanning negatives is to "preserve" all information of
the negative with a linear transfer function, irrespectively if the eye on
the negative or print can see it or not, and then use digital imaging for
the display of all the available image information. Thus, when exposing a
negative for digital scanning, we should capture the contrasts of interest
with the largest possible contrast range. Since good scanners can penetrate
the darkest black of a negative (max. OD = 3-3.5) capturing the details in
the shadows, we should expose more than conventionally done in order of
acquiring also as much detail as possible in the high-lights.

Additionally, one should consider that laser printing (HP LaserJet 4
providing 5-bit gray levels and 100 lines/inch) combined with the
LazarPrint expansion can print 8-bit gray levels at 300 lines/inch, thus
provides a good output for detail rich images at the 1Kx1K level (I tested
recently several printers and reported the results in my home page).



******************************************************************************
* : *
* Klaus-Ruediger Peters, Ph.D. : WWW Home Page: *
* Director, Molecular Imaging Laboratgory : *
* Biomolecular Structure Analysis Center : Molecular Imaging Laboratory *
* University of Connecticut Health Center : http://panda.uchc.edu/ *
* 263 Farmington Ave. : htklaus/index.html *
* Farmington, CT 06030-2017; U.S.A : Differential Hysteresis *
* : Processing Demo at http:// *
* Tel: (203) 679-3977; Fax: (203) 679-1989 : panda.uchc.edu./htbit/indiv/ *
* e-mail: Peters-at-BSAC.UCHC.EDU : /software_docs/dhp.html *
* : *
****************************************************************************
**






From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: Thu, 04 Jan 1996 14:21:55 +0000
Subject: Scanners

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To all,

I got a number of good responses on my question regarding flatbed
scanners. Everybody seemed pleased with the particular one they
owned--there were no clear favorites. A couple of tips: 1) Make sure
your computer has the right output. All of the scanners I have looked at
have a SCSI 2 output while my 2.5-year-old Mac only has a SCSI. I have
been told that I have to upgrade my computer in order to even hook it up to
a SCSI 2 scanner. I'm still investigating this. 2) Make sure you get the
software you need to manipulate the scanned image or text. A lot of
scanners come with software bundles. 3) Look into warranties. Some
devices have none--others are up to 2 years (longer?). 4) Photoshop
comes in two versions--LE and 3.1. The latter is more powerful (and
expensive) than the former. 5) Transparency adapters and document feeders
are usually extra. Both can be useful.--if you need them. 6) Read the
first response (from {sco.umc2-at-Mail.health.ufl.edu} ). It contains a lot of
good basic advice. 7) Good luck.

Bob
________________


One user's opinions:

1. Consider the media you will be scanning. Some scanners do
better at transparencies than others (nikon has a slide scanner
for 3K$), some are designed for large format (Umax has a 17 x 12
inch for 7k$), some do black and white on the cheap (HP has one
B&W for $400).

2. Consider the destination of the image. There is no use
buying a 30 bit high definition scanner if all you will do is
newsprint. A 300dpi 256 gray scanner goes a long way with laser
printers and costs $400.

3. Consider the resolution you need. If you are going to scan
stamps that are 1 inch, and display them at poster size, you need
a very high res scanner (1200 dpi or more) if you are going to
scan 8 x 10 positives and display them in a small window, then
600 is plenty, 300 adequate.

4. Consider the speed. Some scanners take 30 seconds to scan
one page. This can be a real pain if you have any number of
images to scan. Some are available with a document feeder, which
is a nice thing to have, but not if all your scans will be of
pictures (which must be hand fed anyway)
"Single pass" scanning means that all 3 colors (red green blue)
are captured simultaneously--this is faster (HP scanjet 4 offers
this)

5. Real Res. Most scanners interpolate the dpi at high res.
This is ok, but non-interpolated scans are better. Be willing to
pay more for a hardware 600dpi than a software 600dpi.
__________________

Sender: bob-at-befvax.uchicago.edu

What resolution do you need. Many of the inexpensive scanners have a very
small numerical aperature which leads to line broadening. So a step
size of (for example) 5 microns will not resolve a 5 micron line in
single (or two) pixels but rather 10 or more. The requirement for a
large numerical aperature coupled with dimensional stability is
what makes high resolution cost so much.

Bob J.
______________

Response from John Bozzola:

We use a Mac IIci attached to a LaCie 600 dpi color scanner (it's
equivalent to an Epson 300) to occasionally digitize prints. It cost $1,800
- a similar unit may now be purchased for around $600. I have used it for
over 3 years now with no problems whatsoever. 97% of the scanning is done
in the bw mode set to no more than 300 dpi. The program most often used to
drive the scanner is Adobe PhotoShop. As an OCR program, I use WordScan
Plus. It's OK, a bit slow, but it is also 3 years old and a newer version
may be better. I rarely use this program. However, when the documents are
of high quality (typed characters no smaller than 9 point) then it is a
great time saver. There are better OCR programs out there than this one.
_________________

Our Graphics Artist uses a Macintosh computer with a Hewlett Packard scanner
which he likes very much. The one we have is color and has a 400 optical
DPI, although the new Hewlett Packard has a 600 optical DPI. It comes with
Deskscan software and a limited version of PhotoShop. If you want to use
color, though, you need to have the full version of PhotoShop.

Hope this is helpful.

Kathy Stangenberg
Ted Pella, Inc.
_______________

I have a AGFA StudioScan IIsi scanner with Transparency module. I
think that it is a very good scanner. I using it every day.
Some technical data:

- Max. res. 400(H) x 800(V) optical
2400(H) x 2400(H) ppi through interpolation

Sample depth:
10 bits for grey
30 bits for colour

Scan mode: one pass

Scanning speed:
grey 4.5 ms/line
colour 10 ms/line

Scanning area: 316x355 mm (8.5"x14")

MAC PC
SM QM SM QM
Preview colour 16 16 15 15
A4 colour 200 ppi 54 54 32 39
A4 colour 400 ppi 96 104 86 126
15x10 cm colour
400 ppi 44 54 30 38
A4 grey 200 ppi 17 18 12 12
A4 grey 400 ppi 39 42 39 65
A4 line art 400 ppi 20 34 20 34

SM=Speed mode (in seconds)
QM=Quality mode (in seconds)

Software:

- OmniPage Direct OCR
(I suggest you to buy Recognita OCR software, because it is the best as
I think)
- FotoTune
- PhotoShop LE

Price: Scanner + Transparency Module = 250.000 HuF =} 1.700 USD

dr. Peter Kasa jr.

ALBERT SZENT-GYORGYI MEDICAL UNIVERSITY
DEPARTMENT OF PHARMACEUTICAL TECHNOLOGY
6720 SZEGED-HUNGARY

E-MAIL: KASA-at-PHARMA.SZOTE.U-SZEGED.HU
________________


The one chosen to be included in the Kodak Imaging Station is the high end
EPSON with or
without the transparency adapter. I have seen the output from this
equipment rated at an
interpolated 4800dpi , and it is very impressive. When prints are made on
a dye-sub printer
(Kodak or Codonics), it is hard to tell that they were not made from a
negative in a
conventional darkroom.

Regards, Skip
___________________

We are running a Umax Power-look with a transparency scanner for doing
negatives. It came with all the right software including Adobe Photoshop
for either PC or MAC. I think it was about $2600. directly from Umax. We
have been very happy with it so far. Another lab has the same scanner
running on an SGI Indy. Apparently the software is more tricky on that
platform but the results have been very good.

Greg Erdos E-mail: gwe-at-biotech.ufl.edu
______________________

We have an HP Scanjet 3c/T scanner on a Powermac 9500
used for image analysis and presentation purposes. It is
a superb accessory. You can scan virtually anything that
is 8.5 X 11 or smaller with it, although it does not substitute
for a good slide scanner. Since it has the transparency adapter
gels come out very nicely. It has its own miniprogram for
image adjustment, but if you are doing serious work we have
an Adobe suite (Illustrator, Pagemaker & Photoshop) installed
on the Mac and that is what just about everyone uses. For many
types of text (but definitely not all) you do not even need OCR.
If you can afford it, it seems to be the way to go. I am a user and
do not have any financial connection to Hewlett - Packard.

Bruce Cutler, Microscopy Laboratory, Univ. Kansas, Lawrence.
_________________

We have experience with some HP-IIcx's. One is on a Mac, the other on a IBM
PS/1. Both seem to work quite well. They ran about $1000 over a year ago. I
think they support 1200 dpi.

They came with minimal software. The software allowed scanning images well
enough. But optical character recognition required third-party software.
That may all have changed.

Warren E. Straszheim
E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
___________________


I am using a Hewlett Packard ScanJett IIc, about 800 dpi with some
interpolation,
color / bw. This is an "older" model, so I bought a used one for 500 sFr.
(swiss franks).

- OCR even for small printed text (as in journals) is satisfying with OmniPage.
- Microscopic slides *can* be scanned, resulting in about a 30 fold
magnification.

Personally I am very satisfied with this device. Good Luck, Wolf.
______________


We use a UMAX 1260 color flatbed scanner, which will scan to 3000 dpi, and
we love it. It is a three pass color scanner, but the speed isn't slow
enough to justify another $1000 for a one pass scanner. It works as a
plugin to Photoshop.

Also, we use OmniPage Pro for OCR, and again, it's great!

Any questions, just email me.

Scott
________________


Bob, we are using extensively flatbed scanners for digital imaging of our
negatives (fluorescence and histology color LM, TEM and old Polaroids from
my FSEM). My suggestion: Buy an Agfa Arcus II for true 8-12 bit graytone
or 24-48 bit color "output" in TIFF. AGFA is experienced, has excellent
color handling and will be around for a long time for support. The street
price of US$ 2,000 (any US catalog company, i.e., The Mac/PC Zone
1-800-248-0800) includes a "full version" of Photoshop 3.04 which reads and
writes 8-12 bit Tif files. TIFF is the MSA standard. Both versions (PC and
Mac) of Photoshop 3.04 are now available and allow us to work with 12-bit
Tiff's (at my microscope on a PC and at my networked office with a Mac).
The scanner comes with AGFA's FOTOLOOK acquisition software which is opened
from within Photoshop: Files-Acquisition.

Please consider: Not spatial resolution but "contrast resolution" is
important for the acquisition of digital image data, i.e., if you read more
intensity steps you can work with more intensity levels per pixels and can
"see" smaller contrasts. Our experience is that all microscopes can deliver
12-14 bit contrast resolution. The jump from 8-bit to 12-bit gives you an
increase in contrast resolution of 1:16 which is adequate for utilizing the
full range of contrasts found in (TEM, Polaroid) negatives. Their is
already some software out for the handling of 12-bit image data and I
believe that soon many other software packages will available for working
at the level of 12-bit contrast resolution already available in many
microscopes, e.g. AFM microscopes, good CCD cameras for TEM and several SEM
acquisition systems. Even, if you cannot handle 12-bit data at this time
and may have to wait for an upgrade of NIH image, a foreseeing investment
in adequate hardware will pay of very soon when 12-bit contrast resolution
will have a common place in microscopy imaging.

Optimal scanning of negatives requires that the negatives are exposed 1-2
stops more than for ordinary photographic printing (high lights should be
"covered" and not be empty, dark areas should be very dark. Good scanners
can handle more than 3.0 optical density which is "very dense" for a
photographic negative). Also the scanning procedures must be adapted in
order to take advantage of the full contrast transfer function of the
negatives as well as the scanner. I am in the process of putting a report
together at my WWW site on our experience with 8-bit versus 12-bit negative
scanning.
_____________


If black and white prints are to be scanned, the Apple one Scanner (up to 1200
dpi) cost now less than 700 dollars and is very good with OFOTO software.
Equivalent scanners (e.g. LaCie are just a good), but I am not sure about PC
equivalent and would certainly like to know the outcome of recommendations.
Images in the page below were scanned with the Apple one scanner at 300dpifrom
black and white prints without any manipulation (filtering, etc).








From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Fri, 5 Jan 1996 09:00:35 -500
Subject: RE: KMnO4? TEM of Yeasts

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KMnO4 fixation? This is interesting, why are you using KMnO4?

Back in the early 1980's we did a pretty frightening study on KMnO4
fixation in fungi (T. M. Hammill, et al. refs available), in which it
was found that at concentrations of upto 8.5% KMnO4 the test fungi
(Mucor mucedo) continued to grow! (Albeit, abnormally, but ransfer
to fresh media resulted in a return to normal growth). So if these
fungi continue to GROW at 8.5% KMnO4 how good of a "fixative" is
1-3%?


Additionally, Greg, I agree with Bill Tivol's suggestion that the
"swisscheese" effect is due to the fact your resin (1) is too soft -
the chitin cellwalls of fungi are hard, and (2) you are seeing poor
infiltration of your resin - try a lower viscosity resin, I've had
excellent results with fungi using Spurr's Hard or Quetol 651/MNA
(Kushida, 1975).

Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu

int.




From: minter-at-kodak.com (John Minter)
Date: Fri, 5 Jan 1996 08:43:34 -0500
Subject: Negative scanning

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I would like to add a couple of comments to the recent thread about
negative scanning.

Several people have mentioned using moderately priced flat-bed scanners
with transparency adapters. My evaluation of one of these (Epson Scantastic)
was that it was useful for quick prints of light (density 1 or less)
negatives to show qualitative microstructure. This unit had a
non linear response to density, especially over 1. This made the output
unsuitable for quantitative analysis and often resulted in "posterization",
where large areas with visible contrast differences were all mapped to
the same gray level. Check for this by scanning a density step tablet.
Scanning periodic features in the negative (i.e. lattice images) resulted
in moire patterns (check the FFT of some of your images).

As a result, we chose not to purchase this scanner and added a new
PC interface to our old Optronics P1000 rotating drum scanner. With
the addition of a new module for the log amplifier that permits a wider
range of gain and DC offsets, we can select linear ranges of density
of 0.5, 1.0, 2.0, 3.0, and 4.0 with a variable offset of up to 1.5
density units. (This all was supplied by CSI, a small company in England).
This provides the ability to get the most significant 8 bits of data per
pixel and is free from interference effects.

For the highest resolution, we send the negatives to another lab which
has a Perkin Elmer flatbed microdensitometer. These scans are very slow,
but have the potential of very high resolution (we have scanned down to
5 microns t0o be certain we oversampled the film).

Best Regards,
John

John R. Minter, Ph. D. Phone: (716) 722-3407
Eastman Kodak Company FAX: (716) 477-3029
Analytical Technology Division email: minter-at-kodak.com
Rochester, NY 14562-3712






From: kennedy-at-nsi.edu (grace kennedy)
Date: Fri, 5 Jan 1996 08:50:04 -0800
Subject: tissue water content

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A colleague needs to accurately determine the water content of very small
tissue samples (fish embryos at various stages of development). What would
be the best way to do this? Thanks for any suggestions. Grace






From: Ronald Cohn (415) 8556059 :      Ronald.Cohn-at-syntex.com
Date: Fri, 05 Jan 1996 10:05:53 -0800 (PST)
Subject: LM - immuno background problems

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Microscopists,

Does anyone on the list have tried-and-true methods for eliminating or
minimizing background while immunolabeling paraffin-embedded lung tissue? My
attempts to label a protein in rat lung using the Vector avidin-biotin alkaline
phosphatase kit and the Vector Red substrate development kit have resulted in
what I suspect is specific labeling, but also unacceptably high background.
Control experiments in which 1) primary antibody has been omitted, 2) spurious
avidin binding has been blocked by use of an avidin/biotin blocking kit, and 3)
the potential presence of endogenous alkaline phosphatase was blocked by
levamisole, have not reduced the background. The background appears to be
primarily over connective tissues, and develops simultaneously with specific
label.

Any suggestions will be greatly appreciated. If you would respond directly to
me, I will compile all reponses and post a summary in a few days. Thanks in
advance!

Ron Cohn
Roche Bioscience
ronald.cohn-at-syntex.com






From: Doug Davis :      doug_davis-at-maillink.berkeley.edu
Date: 5 Jan 1996 11:04:51 -0800
Subject: TEM and ID of G+/G- bacteri

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Message-ID: {n1391282501.36904-at-maillink.berkeley.edu}

Subject: Time: 12:09 PM
OFFICE MEMO TEM and ID of G+/G- bacteria Date: 1/5/96

To all:
Is there a definitive EM method of determining whether an unknown bacterium
(in this case a plant pathogen) is G+ or G-? This one gives ambiguous
results at the LM level. TIA.

Doug Davis doug_davis-at-maillink.berkeley.edu
EML
UC Berkeley





From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Fri, 5 Jan 1996 10:07:16 -0500
Subject: Re: TEM of yeast

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} To: William Tivol {tivol-at-wadsworth.org}
} From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
} Subject: Re: TEM of yeast
}
} } }
} } } I am looking for a protocol for the embedding of yeast for
} } } TEM. I made a few attempts and the results have been
} } } mixed. The biggest problem is that the cut sections look
} } } like Swiss cheese. Where yeast should be there are holes.
} }
} } Dear Greg,
} } I had the same problem with pollen grains, and it arises from the
} } difference in hardness between the specimen and the resin. Our solution
} } was to experiment with different proportions of the constituents of the
} } resin until its hardness matched that of the specimen. Of course, this has
} } to be a trial-and-error process. Good luck.
} } Yours,
} } Bill Tivol
} }
} } Greg,
} I've got a protocol that works well with yeast
(infiltration/embedding):
}
} 1)Fix in suspension 4%pf/2%ga 3hrs rotating. Spin after ~13K 10 sec.
} 2)Rinse in buffer 3 x 10 min (last rinse should be cacodylate)
} 3)Postfix 2-4% KMNO4 in caco, on ice 1 hr
} 4)3 X 5 min D-H2O
} 5)en-bloc 2%UA filtered aq 1hr dark
} 6)Quick 50% ETOH then break up pellet into poppy seed size granules
} 7)50-70-90% ETOH 5 min ea
} 8)3 x 100% ETOH
} 9)1:1 Spurrs:ETOH overnight rotating
} 10)Vac infiltrate 15 psi fresh Spurrs R.T. 2 hrs
} 11)Rotate fresh Spurrs 2 hrs
} 12)Repeat step 10
} 13)Change Spurrs and vac infiltrate 15 psi 30 min
} 14)Turn on oven cure o\n 60 degrees 15 psi
}
} You will need an oven that can be pumped down, if not use house vac.
} The KMNO4 extracts some ribosomes so that you can see organelles better
} than you would with Osmium. Don't fix too many cells, a 1-3 mm pellet
} in a 1.5 ml eppy is plenty. Of course your adding a 2x fix at equal
} vol to the suspension to get the final concentration in step 1. Not
} all samples will fix optimaly so you can play with fix conc. and time.
} Microwave fixation is an option to cut time down.
} Good Luck
} Mike D.
}
}





From: kennedy-at-nsi.edu (grace kennedy)
Date: Fri, 5 Jan 1996 11:12:48 -0800
Subject: water content II

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I forgot to mention in my former query that the obvious method of weighing,
drying, weighing again is not practical with these samples. They are too
small and delicate to accurately removes excess water from the outside--the
gross error is too large. Thanks Grace






From: mmdisko-at-erenj.com (Mark M Disko)
Date: Fri, 5 Jan 1996 10:58:23 -0500
Subject: Postdoctoral position TEM, Exxon R & E

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Posted-Date: Fri, 5 Jan 1996 10:58:23 -0500
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POSTDOCTORAL POSITION IN ELECTRON MICROSCOPY
AND MATERIALS SCIENCE OF CATALYTIC MATERIALS

Corporate Research Laboratory
Exxon Research and Engineering Company.

The position will involve the use of high resolution and
analytical electron microscopy for understanding model
supported catalysts, and for developing new catalysts used in
refining or chemical production. Requirements include
expertise with high resolution electron microscopy, electron
holography, field-emission analytical microscopy, and
scattering physics. Experience with the materials science of
catalysts is desirable for this rapidly advancing research
program. Research in this area involves a multidisciplinary
team approach which provides an excellent learning environment.
Our laboratory offers advanced instrumentation for
structure-property studies of catalysts, including a new
field-emission TEM equipped with a rotatable electron biprism
for electron holography. We have extensive software and
computer systems available for the computational component of
this research. Candidates should have experience with digital
imaging using CCD systems, and experience with or knowledge of
the analytical methods for processing electron holograms.

The term of the position is one year beginning early in 1996
with a likely extension to two years. Our research lab in
rural New Jersey offers convenient access to Philadelphia,
Princeton and New York City. Exxon offers an excellent
working environment, salary commensurate with skills and
experience, and excellent benefits.

Applicants for this position who meet the majority of the
qualifications outlined above should forward a resume,
publication list and two or three letters of reference to:

Dr. Mark M. Disko
Corporate Research Laboratory
Exxon Research and Engineering Company
1545 Route 22 East
Annandale, New Jersey 08801

(908) 730-2503
FAX (908) 730-3314

Please do not respond directly to this list server. In the
event you need further information rapidly, forward electronic
mail to me at mmdisko-at-erenj.com .

Equal Opportunity Employer M/F/H/V







From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 5 Jan 1996 13:51:32 -0500
Subject: K-11 fixer

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Message-Id: {n1391272195.84003-at-msmail.tmc.tulane.edu}

Someone approached me today for details on a K-11 fixer, that I never used.
Any info. ref. will be appreciated.

******************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} Prof. Pathology & Otolaryngology *
* http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html *
******************************************************************






From: em-at-mediacity.com (E. Monberg)
Date: Fri, 5 Jan 1996 10:29:06 -0800
Subject: tissue water content

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} From: kennedy-at-nsi.edu (grace kennedy)
} Subject: tissue water content
}
} A colleague needs to accurately determine the water content of very small
} tissue samples (fish embryos at various stages of development). What would
} be the best way to do this? Thanks for any suggestions. Grace
}


Gross Analysis: Weigh,
Freeze Dry
Weigh again.

The difference in weights = H2O Content.










Regards,


Ed Monberg, GM em-at-mediacity.com
LMDC (Laser Motion Development Company)
3101 Whipple Road Union City, CA 94587-1216
510-429-1060 voice
510-429-1065 fax


Inventory Catalogue Listing:

Send empty e-mail to: Cat-at-LaserMotion.com






From: STANSMAN-at-aol.com
Date: Sat, 6 Jan 1996 11:19:54 -0500
Subject: Re: Scanners

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boundary="PART.BOUNDARY.0.17542.mail06.mail.aol.com.820945190"


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Hello all,

The recent mail threads concerning flatbed scanners and negative scanning
provided very good information on the resolution and contrast requirements
needed to satisfy various imaging formats. I would like to provide for
informational purposes only technical specifications on two Nikon scanners
that have not been mentioned.

If you do not wish this to view this information please don't download the
attached file.

Regards,

Stan Schwartz
Nikon Inc.

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SCANTOUCH: $1,535 SRP
=0D
Gives Your Images That Professional Touch
=0D
If you spend a lot of time scanning, you'll appreciate Nikon's fast,
versatile, and affordable high-resolution color scanner. Backed by Nikon'=
s
renowned design and quality, the ScanTouch flatbed scanner offers the hig=
h
level of performance and value you demand. Take for instance its outstand=
ing
1,200 dpi resolution with a super-fast scanning method. And professional
scanning software for both Macintosh and IBM computers. Plus other rich,
image-enhancing features ideal for your professional-quality layouts and
publications using both images and text.
=0D
High resolution: 1,200 x 1,200 dpi
Featuring 24-bit full color with an optical resolution of 565 x 1,200 dpi=
,
the Nikon ScanTouch offers an impressive 1,200 x 1,200 dpi hardware
interpolated resolution. If you require finer image detail for line art a=
nd
graphics, its integrated powerful driver software provides a software
interpolated resolution of 2,400 by 2,400 dpi or higher.
=0D
10-bit internal processing per color
When it comes to detailed color reproduction, the Nikon ScanTouch makes n=
o
compromises. A 10-bit A/D conversion for each color (R, G, B) with ColorS=
ync
compatibility ensures that your photos and artwork are accurately reprodu=
ced
with fine color gradations.
=0D
Three scanning modes
Because requirements vary from job to job, the ScanTouch offers three
versatile modes for speed and quality (A4-size, 300 dpi full color): Norm=
al
Mode (approx. 60 sec.); Fast Mode (30 sec.); and High-Quality Mode (appro=
x.
120 sec.).
=0D
Compatibility with your personal computer
The ScanTouch comes ready to use with popular scanning packages - Photosh=
op
plug-in for Mac and PC and TWAIN compliant drivers (for PC only).
=0D
Fastest-class color preview
Just as important to productivity as great image quality is being able to=

preview your scanned images quickly. At 12 sec. per page (A4), ScanTouch
provides one of the fastest color preview times in its class.
=0D
Wide scanning area
A wide surface scanning area of 8.5 x 14 in. lets you use the ScanTouch f=
or
most standard paper sizes such as A4, US Letter, and Legal.
=0D
Multifunctional, easy-to-use driver software
Supports four types of output: line art, halftone, grayscale and full col=
or.
Available with automatic exposure, manual gamma compensation, manual gamm=
a
simulation, as well as cropping. You can also customize resolution and im=
age
specifications and adjust sharpness processing to emphasize contours and
blurring processing to prevent moire effect when scanning printed materia=
ls.
=0D
SCSI-II compatibility
For fast transfer rate of image data to your computer, this
high-quality scanner supports the advanced SCSI-II standard.
=0D
High character resolution for OCR applications
When used with standard commercial OCR (optical character recognition)
software, ScanTouch provides quick and accurate character reading.
=0D
Bundled software
Comes with Caere's OmniPage Direct OCR software and Light Source's Ofoto,=
an
imaging application featuring automated scanning functions, color calibra=
tion
and image editing capabilities. (Note: Nikon will temporarily be providin=
g
Photoshop LE instead of Ofoto for the Macintosh model Scantouch. Ofoto wi=
ll
be made available at a later date, free of charge. However, use of Ofoto =
will
require a modification to Scantouch. There will be a service fee for this=

modification. Please contact Nikon's Service Department -at- 516-547-4351 fo=
r
further information).
=0D
Specifications
- Original Document: Reflective original (printed/photo), max. 8.5 x 14 i=
n.;
Transparency or negative original (35mm to
5 x 5.7 in. film, at up to 1,200 dpi), Max. size with full rotation: 4 x =
5
in.
- Reading Method: Fixed original, moving optical unit flatbed
scanning
- Sensor: CCD linear image sensor
- Light Source: Fluorescent lamp
- Color Separation: 3-pass RGB
- Effective Reading Area: Reflective Original 8.5 x 14 in. (216 x 356mm);=

Optical Resolution: 565 x 1200 dpi; Interpolated Resolution: 1,200 x 1,20=
0
dpi (hardware), 2,400 x 2,400 dpi (software)
- A/D Conversion: 10 bits/color
- Output Data: Line Art, Halftone: 1-bit/pixel; Grayscale: 8 bits/pixel; =
Full
Color: 24 bits/pixel
- Reference Reading Time (A4 300 -dpi Full Color): Approx. 30 sec. (Fast
Mode, without exposure compensation)
- Interface: SCSI (Complies with SCSI-II)
- Dimensions (W x H x D): 14.8 x 6.3 x 23.6 in. (376 x 159 x 599mm)
- Weight: Approx. 26.5 lbs. (12kg)
- Power Requirements: AC 100 - 240V, 50/60Hz, max. 50W
- Environmental Conditions - Temperature: +50xF to +95xF (+10xC to +35xC)=

=0D

Optional Transparency Unit AT-45: $675 SRP
To scan transparencies and negative film (35mm to 5 x 5.7 in. film, at up=
to
1,200 dpi), Nikon offers a low-profile, lightweight transparency unit tha=
t's
remarkably easy to use.
=0D

All names of companies and products are trademarks or registered trademar=
ks
of their respective holders.
=0D
Specifications and equipment are subject to change without notice or
obligation on the part of the manufacturer. June, 1994.
=0D
LS-4500AF Multi-format Film Scanner
SRP: $11,295
=0D
New Technology for Enhanced Productivity
=0D
* Supports formats from 35mm to 4x5 in.
* Scanning head with dual-optical system
* Precision 12-bit A/D conversion maintains wide dynamic range =

* Autofocus ensures consistently sharp scans
* 360x-rotation film carriers with masks for all popular frame sizes
streamlines production =

* Easy-access front-loading design simplifies film handling
=0D
One scanner for multiple formats
=0D
The new Nikon multi-format scanner can handle popular film sizes from 35m=
m up
to 4x5 in. It features 12-bit A/D conversion, 3000 dpi resolution on 35m=
m
and 1000 dpi on 4x5, and it's small enough to fit on your desktop! The
LS-4500AF has an automatic energy-saving cycle and can handle your most
demanding high-end film scanning requirements. =

=0D
Dual-optical CCD scanning heads support multiple formats
=0D
Now, a single device can fulfill your multi-format needs. Nikon solved t=
he
challenge of matching film size to scanning engine by combining two scann=
ing
systems within the same device. The result is a scanner with high resolu=
tion
and performance, giving you the quality you need for all of its formats.
=0D
High-performance signal processing and mechanical accuracy
=0D
Preserving the wide dynamic range found on commercial transparencies, the=

LS-4500AF is ideal for professional applications. Color accuracy is tigh=
tly
controlled through 3D color matrix processing in hardware. The LS-4500's=

DSPs provide the power to scan and process any final size up to 3000 dpi =
in 1
dpi increments, even from panorama formats! Its native scanner intellige=
nce
handles a wide array of positive and negative film types, formats, color
masks and dye sets. =

The LS-4500's unique illumination path ensures a compact desktop footprin=
t,
and provides uniform edge-to-edge brightness, regardless of the film form=
at,
or its particular angle of rotation in the film carrier. High-definition=

optics overcome the problem of geometric distortion, enabling 3000/1000 d=
pi
high-quality output. With a precision-machined ballscrew drive, LS-4500A=
F

delivers remarkably accurate color registration.
=0D
Easy-to-use front loading design
=0D
Film handling with the LS-4500AF is a streamlined operation cutting hours=

from your scanning production time. With a versatile carrier and mask se=
t,
film can be quickly mounted, rotated to a precise angle, and inserted int=
o
the autoloading slot. It is positioned by a special servo system to
precisely locate the film in the optical path. You can be scanning withi=
n
seconds, confident that the alignment and focus are accurate.
=0D
High-speed scanning and preview
=0D
The LS-4500AF scans fast - 120 seconds for full-frame 35mm at 3000dpi, an=
d
180 seconds for full-frame 4x5 at 1000 dpi. Prescan is only 30 seconds o=
n
4x5 and 20 seconds on 35mm. In addition to precise servo-controlled manu=
al
focus, Nikon's autofocus takes care of precision focusing for you. This
scanner is suited to large-volume pre-press scanning operations. LS-4500=
AF
can breeze through complex assignments, putting the profit back into your=

scanning operation.Easy-to-use Nikon software
=0D
The LS-4500AF is also an easy scanner to use, giving you the comprehensiv=
e
controls you need for professional results. Bundled with the scanner, Ni=
kon
introduces a completely re-architected Photoshopx plug-in for Mac OS, and=

TWAIN source for Windows users. The new Nikon Scan driver runs with any
Photoshop or TWAIN-compatible image editing software, or standalone, usin=
g
the Nikon Control application shell.
=0D
Nikon Scan Driver Software Requirements
=0D
Operating System Mac OSr / Macintosh Windowsr
Computer Platform 68xxx CPU without FPU, or PowerPCx CPU IBMr PC-AT
compatibles (386, 486, or Pentiumx CPU
RAM Plug-in requires a minimum of 2MB free RAM, Virtual Memory and Modern=

Memory Manager compatible. Image editing applications typically require =
a
minimum of 5-8MB RAM. With system memory requirements exceeding 2MB, tot=
al
recommended RAM should be greater than 16MB for productive scanning. TWAI=
N
module requires a minimum of 2MB free RAM, Virtual Memory compatible. Im=
age
editing applications typically require a minimum of 5-8MB RAM. With syst=
em
memory requirements exceeding 2MB, total recommended RAM should be greate=
r
than 16MB for productive scanning.
Hard Disk Installation requires a minimum of 1MB free space. 300 MB or
larger disk is recommended for scanning operation Installation requires a=

minimum of 1MB free space. 300 MB or larger disk is recommended for scan=
ning
operation
Display 640 x 400 (or larger) full color (24-bit) display recommended 640=
x
480 VGA (or larger) full color (24-bit) display recommended
Interface SCSI-II ASPI compliant board supporting WINASPI.DLL
OS Version System 7.0 or later, in English, German, French versions
* MS-DOSr version 5.0 or later (requires enhanced mode)
* IBMr-DOS version 5.0 or later (requires enhanced mode)
* MSr-Windows version 3.1 or later (Win16 environment), English, German,
French versions
=0D
Nikon Scan Driver Software Features
=0D
Scanner source selection, source image type selection, resizable dialog b=
ox,
resizable preview, autoexpose, autofocus, manual focus, crop, zoom,
resolution, resize, fiducial reference scale, pixel address coordinate
display, on screen densitometer, sharpening, analog exposure, analog colo=
r
balance, contrast, brightness, color balance, white point, gamma curve ed=
it,
histogram, black point, final scan, eject, instant screen update on densi=
ty
and color adjustment, interactive helpLS-4500AF Specifications
=0D
1. Reading System/Optics
=0D
Film types: 4x5 in.; 40mm, 65mm, 75mm, and120/220 formats, including 6x4.=
5,
6x6, 6x7, 6x9, up to panorama formats; 35mm film (single frame, 6-frame
strip, mounted film)
Transparency, positive or negative, color or monochrome
Reading resolution: 5000-pixel monochrome linear CCD x 2
[A]: 1000 dpi reading resolution
[B]: 3000 dpi reading resolution
Note: Dual-optical system modes are indicated by [A] and [B] in this docu=
ment
No. of pixels [A]: Maximum 5000 x 12000 pixels
[B]: Maximum 5000 x 18000 pixels
Effective scanning area [A]: 5 in. (Main scan) x 6 in. (Sub-scan)
[B]: 42mm (Main scan) x 6 in. (Sub-scan)
Light source: 12V- 20W halogen lamp
Color separation: RGB frame sequential
Film carrier: The following film formats are supported by three types of =
film
carriers, and masks are used to mount the film according to aperture/fram=
e
size. All carriers can be manually rotated for exact alignment during sc=
an,
except the 35mm 6-frame strip carrier; 4x5 in. sheet film; 6x4.5, 6x6, 6x=
7,
6x9cm cut frames; 35mm cut frames (three-up) 35mm mounted slides (four-up=
);
35mm x 6 frame strip
Imaging optics:
[A]: 8 lenses in 4 groups
[B]: 6 lenses in 4 groups
Autofocus: Contrast detection by CCD, focusing area selectable, manual
focusing by software-controlled servo
=0D
2. Scanning/Signal processing
=0D
Image scanning Three-pass RGB
Main scan: 5000-pixel monochrome linear CCD
[A]: 5000 pixels (hardware-interpolated to 10000)
[B]: 5000 pixels
Sub-scanning: stepper driven film stage
[A]: 3 steps / line (2000 dpi)
[B]: 3 steps / line (3000 dpi)
Scan time:
[A] Pre-scan: Approx. 30 seconds; Final scan: Standard mode - Approx. 210=

sec, Medium-speed mode - approx. 180 sec
(-at- 1000 dpi, 4500 x 3600 pixels for approx. 46.3MB)
[B Prescan: Approx. 20 seconds; Final scan: Standard mode - Approx. 200 s=
ec,
Medium-speed mode - approx. 120 sec
(-at- 3000 dpi, 3900 x 2600 pixels for approx. 29MB)
Scanning spatial density: Pixel density:
[A]: maximum 2000 dpi interpolated from 1000 dpi
[B]: maximum 3000 dpi
Pixel size: [A]: 12.7um square
[B]: 8.5um square
A/D conversion: 12-bits per color channel
Output data: 8-bits per color channel
=0D
3. Data transfer
=0D
Panel indicators:READY, BUSY, and ERROR states indicated by LED
Scanning software: Photoshop plug-in for Mac OS and TWAIN source for Wind=
ows.
NikonScan application supports Photoshop plug-in and TWAIN source on both=
Mac
OS and Windows platforms
Interface: SCSI-II
Image transfer: Three-pass RGB frame sequential
Maximum transfer rate: 1 MB/sec, or better
=0D
4. Operating conditions
=0D
Power requirements: 100 - 120VAC/200 - 240VAC; 0.8A/0.4A, 50/60Hz
Environmental Temperature: 10xC - 35xC (50xF - 95xF)
Relative Humidity: 30 - 85% (non-condensing)
Dimensions and weight: =

295 (W) x 420 (D) x 250 (H)mm; approx. 13kg
11.6 (W) x 16.5 (D) x 9.8 (H) in.; approx. 28.7 lb.
=0D
Macintosh is a registered trademark and Mac OS is a trademark of Apple
Computer, Inc.
MS, MS-DOS and Windows are registered trademarks of Microsoft Corporation=

Photoshop is a trademark of Adobe Systems, Incorporated
IBM is a registered trademark, and PowerPC is a trademark of Internationa=
l
Business Machines Corporation
Pentium is a trademark of Intel Corporation
=0D
Contacting Nikon is Easy!
=0D
In the US, for product literature and the name and number of a dealer nea=
r
you, call 800-52-NIKON. For Marketing, Technical and Service support for=

Nikon Electronic Imaging Products in North, Central, and South America pl=
ease
contact Nikon EID personnel at:
=0D
Nikon Inc.
Electronic Imaging Department
1300 Walt Whitman Road,
Melville, NY 11747-3064
Attention: Marketing, Technical Support or Sales =

Phone: 516-547-4355
Fax: 516-547-0305
800: 800-52-Nikon
America Online: Keyword Nikon
CompuServe: Go Nikon
=0D
For all other regions, consult the list below to find the Nikon subsidiar=
y
closest to you:
=0D
Nikon Corporation
Electronic Image Engineeering Division
Fuji Bldg., 2-3 Marunouchi 3-chome,
Chiyoda-ku, Tokyo 100, Japan
Phone: 81-3-3216-1034
Fax: 81-3-3214-8193
=0D
Nikon Canada Inc.
1366 Aerowood Drive,
Mississauga, Ontario, Canada L4W 1C1
Phone: 416-625-9910
Fax: 416-625-0103
=0D
Nikon AG
Kaspar-Fenner Strasa 6,
8700 Kunacht/ZH, Switzerland
Phone: 41-1-913-61-11
Fax: 41-1-913-63-63
=0D
Nikon GmbH
Tiefenbroicher Weg 25,
40472 Dusseldorf, Germany
Phone: 49-211-9414-0
Fax: 49-211-9414-330
=0D
Nikon UK Ltd.
Nikon House
380 Richmond Road,
Kingston-upon-Thames,
Surrey, KT2 5PR, United Kingdom
Phone: 44-181-541-4440
Fax: 44-181-541-4584
=0D
Nikon France S.A.
191, rue du Marche Rollay,
94504 Champigny-sur-Mame, France
Phone: 33-1-45-16-46-00
Fax: 33-1-45-16-00-33
=0D
Anam Instruments Co., Ltd.
197-7 Guro 3-Dong,
Guro-Ku, Seoul, Korea
Phone: 82-2-460-5032
Fax: 82-2-861-4895
=0D
Lin Trading Co., Ltd.
2F. No. 270, Sec 3., Nanking E. Road, =

Taipei, Taiwan, Republic of China
Phone: 886-2-740-3366
Fax: 886-2-773-5577
=0D
Shriro (H.K.) Ltd.
2nd Floor, Hutchison House,
10 Harcourt Road (G.P.O. Box 181), Hong Kong
Phone: 852-2524-5031
Fax: 852-2810-6586
=0D
Shriro (Singapore) Pte. Ltd.
Shriro House
11 Chang Cham, Singapore 0315
Phone: 65-472-7777
Fax: 65-472-1792
=0D
Shriro (Malaysia) SDN BHD
Lots 22 & 24Julan 225, Section 51A,
46100 Petaling, Jaya, Selangor, Daryl Ehsan,
Malaysia (P.O. Box 10571, 50718 Kula Lumpur)
Phone: 60-3-774-9842
Fax: 60-3-775-2463/775-2400
=0D
Maxwell Optical Industries Pty Ltd.
Unit 27, Level A, 100 Harris Street Pyrmont N.S.W
2009 Australia
Phone: 61-2-660-7088
Fax: 61-2-660-8739
=0D
T.A. Macalister Limited
Private Bag 92146, Aukland, New Zealand
Phone: 64-9-303-4334
Fax: 64-9-309-6502

--PART.BOUNDARY.0.17542.mail06.mail.aol.com.820945190--





From: Norm Granholm :      Norman.Granholm-at-uc.edu
Date: Sun, 07 Jan 1996 09:48:50 -0500 (EST)
Subject: Re: Scanners

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help





From: user image :      schmutzm-at-lear.u-strasbg.fr
Date: Mon, 8 Jan 1996 10:43:26 +0100
Subject: Cell culture in SEM

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HI everybody,

Best wishes for this new year, hope that you will receive nice pictures...


There is a guy here in Strasbourg, who wishes to have a look on his oligodentrocytes in culture
by SEM. For these purpose he uses antibodies coupled to gold to label the cells.

My question is, what is the minimal size of the gold particles to have a chance to see them by SEM
and the best way to prepare the sample (metallisation or not, carbon spputering, tension etc...) for SEM.

The SEM we have is a XL 20 from Philips.

Any related litterature is welcome as we are novice in this field.

TIA


Marc Schmutz



______________________________________

Dr. SCHMUTZ Marc
Electron microscopy lab.
IGBMC
BP 163
F67404 ILLKIRCH Cedex
France

Phone: 33 88 65 33 32
Fax: 33 88 65 32 01
email: schmutzm-at-lear.u-strasbg.fr

--------------------------------------






From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Mon, 08 Jan 1996 09:43:06 +0000
Subject: water content II -Reply

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Message-Id: {s0f0e6e8.094-at-wpo.nerc.ac.uk}
X-Mailer: Novell GroupWise 4.1

Dear Grace

There is a method of estimating water content of specimens if you can do
TEM with X-ray microanalysis and work carefully; after the method of
Ingram FD & Ingram MJ (1986) Cell volume regulation studies with the
elctron microprobe. In: The Science of Biological Specimen Preparation for
Microscopy and Microanalysis (eds. J-P Revel, T Barnard and GH Haggis)
pp.139-146. SEM Inc. Chicago.

It means working on sections and may be too detailed for what you need.

I did something similar, using the chlorine peak of Spurr's resin to represent
100% hydration, and then lesser peak counts to represent replacement of
the water by tissue components. Tissue chlorine is normally pretty labile
and is lost in processing.

Keith Ryan
PLymouth Marine laboratory
Citadel Hill
Plymouth PL1 2PB, UK






From: Marc D'Olieslaeger :      mdoliesl-at-luc.ac.be
Date: Mon, 8 Jan 1996 13:33:52 +0100 (MET)
Subject: water content II -Reply

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unsubscribe microscopy mdoliesl-at-luc.ac.be




From: Norman Charnley :      norman-at-earth.ox.ac.uk
Date: 08 Jan 1996 12:28:35 +0000
Subject: Image transfer and re-use

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To: microscopy-at-Sparc5.Microscopy.Com


I am looking for advice and pointers in the realm of image transfer
and manipulation, and I know this forum has a wealth of people's
experiences available.

The department here has a number of instruments which can generate
images of various sorts - optical (light microscope), element maps
(SEMs/EMPA), secondary/back-scattered electron (SEMs/EMPA), isotope
maps(SIMS).

Any one sample may pass through several of these instruments, so that
the same area can be examined using different techniques. At the moment
images are transferred as hardcopy photographs or plots. We are starting
to investigate the possibilities of storing images electronically,
transferring them between instruments by wire rather than as hardcopy,
then redisplaying them and relocating areas of interest. (We are also
going to have to consider the problem of longer term image archiving,
which has recently been subject to useful discussion on the list.)

Obvious questions immediately occur:

In what format should the images be stored? TIFF seems to be a current
common standard for this sort of work, but are there better alternatives?
Are there any reviews/publications which would help? (We are also going
to have to consider the problem of longer term image archiving, which has
recently been subject to useful discussion on the list.)

Each instrument's specimen stage has its own particular coordinate
system. Relating coordinate systems should be possible, given
reference locations on the samples, but how to define such reference
points - using distinctive features, or by marking the specimen
somehow?

Other laboratories must be doing this sort of thing - how have they
got on, what are the problems? If they could start again, how would they
change things? What do we need to look out for, and take particular
care of?

This posting is one of the first steps on what is clearly going to be a
long term project. I look forward to the sort of interesting responses
and discussion which commonly arise in this mailing list.

Norman Charnley


======================================================================
Dr. Norman Charnley Tel: +44 1865 272012 (Laboratory)
Electron Microprobe Laboratory +44 1865 272053 (Office)
Department of Earth Sciences Fax: +44 1865 272072
University of Oxford
Oxford OX1 3PR, UK. E-mail:Norman.Charnley-at-earth.ox.ac.uk





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 08 Jan 96 09:28:09 EST
Subject: Re: Cell culture in SEM

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We have a technical paper available on colloidal gold immunolabelling and SEM at
our Web site.
The URL is:
http://www.mwrn.com/ebs/ebs.htm
I hope you find it helpful.
Steven Slap, Vice-President





From: wise-at-vaxa.cis.uwosh.edu
Date: Thu, 04 Jan 1996 14:07:07 +0000
Subject: "Antique" microscopes

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Message-ID: {n1391031777.18894-at-qmgate.anl.gov}
wise-at-vaxa.cis.uwosh.edu
X-Mailer: Mail*Link SMTP-QM 3.0.2

RE} "Antique" microscopes 1/8/96

Good morning. For your information, there is a society which I belong to that
is called The Microscope Historical Society, 14 Tall Acres Dr., Pittsford, NY
14534 that can help you. Also, there are dealers which focus on antique
microscopes . Contact Conrad Schure at 908.431.5191 about their next
instrument fair in February at Hyatt Regency Hotel in New Brunswick, NJ.

--------------------------------------

Bob


Robert R. Wise, PhD
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu



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From: wise-at-vaxa.cis.uwosh.edu
Date: Mon, 08 Jan 1996 12:08:20 +0000
Subject: "Antique" microscopes

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To all,

Thank you for all the responses to my request regarding possible
home for used light microscopes. Apparently, there is a market for
reasonbly priced scopes. I received several names of people to contact and
20 requests (18 email, one phone and one fax) for the 12 scopes we have.
Many of the respondents wanted more than one scope. I have kept all
requests and will talk to our purchasing agent to see what can be done.
I'll get back to you all when I know more.

Bob


Robert R. Wise, PhD
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: randy nessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Mon, 8 Jan 1996 11:39:35 -0600 (CST)
Subject: Increased LN2 usage of EDS detector?

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Hello,
It seems that one of our EDS detectors consumption of LN2 has
increased as of late. I am making the assumption that possibly the vacuum
needs regeneration in the dewar. Does anyone know where one might acquire
the fitting to interface a vacuum system to the dewar of a Kevex detector?
Thank you in advance,
Randy Nessler






From: William R Oliver :      oliver-at-ipas4.afip.mil
Date: Mon, 8 Jan 1996 11:22:50 -0500 (EST)
Subject: Re: Image transfer and re-use

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On 8 Jan 1996, Norman Charnley wrote:

}
}
} Obvious questions immediately occur:
}
} In what format should the images be stored? TIFF seems to be a current
} common standard for this sort of work, but are there better alternatives?
} Are there any reviews/publications which would help? (We are also going
} to have to consider the problem of longer term image archiving, which has
} recently been subject to useful discussion on the list.)

Well, for my two cents worth, my opinion is that format is almost
irrelevant, except for a couple of points. The reason for this is
that there are numerous utilities for moving between formats, both
public domain (such as the pbmplus and netpbm libs) and commercial.

Some formats have standardized compression methods, and many don't,
but even that doesn't really matter in most cases since you can
compress things without relying on the format. For instance,
you can use LZW compression as a part of the TIFF file, or you can
save it unformatted and then compress the file using a compression
routine. For my images, the difference in efficiency between the
two approaches has been small. Certainly, some methods incorporate
more efficient compression than others, but still, I have found
the practical effect of, say, 45% compressions versus 40% compression
to be of little importance for the number of images I manipulate.
You can certainly obsess over each little bit of efficiency, and
there are a number of religious arguments for a number of methods,
but I have found the differences of little practical value for
my archives.

With the differences between the formats of minimal interest to me
(with a few exceptions listed below), then the most important thing
to me becomes what *manipulation* software I use for which set of
images. For instance, I have one package that can read and write
TARGA files easily, but reads and writes TIFF images slowly and with
occasional errors. For images I use this software on, I use TARGA
files. A different program does a better job with Sun raster format.

I tend to save all my images in TARGA, but that is because it seems
to be read and written faster and with fewer errors for the
software I use. But, frankly, it really doesn't matter. I suggest
you first decide on what software you plan to use to manipulate your
images and *then* worry about format.

With all of this, there are a couple of things that become
important quickly:

1) Make sure you can save your images with enough depth. I generally
use 24-, 32- and 48-bit images. Thus, formats which do not support
at least 24 bit "truecolor" representation are useless for me. On
the other hand, if you are going to be happy with 8-bit images, then
you have greater flexibility. I don't think there is a 24-bit GIF
format, for instance, and relatively few formats will easily allow
you go move to 32-bits (Targa supports it well).

2) Make sure you use lossless compression if you plan to do
any image enhancement. Lossy compression, such as jpeg, can
destroy an image for enhancement -- you end up enhancing the
compression artifacts instead of the data. Jpeg and fractal
compression are notorious for this.

Given these two caveats, then, I suggest you first look at the
software you plan to use for image manipulation, and then choose
a format that fits that software nicely.


billo





From: loewe-at-uni-bonn.de (Andreas Loewe)
Date: Tue, 9 Jan 1996 09:43:55 -0500
Subject: TEM EM300 available (in Germany)

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Hello friends of electron microscopy,

we have a Phillips EM300 from 1971 to sell. It was well serviced throughout
its existence in our department. It is perfectly suited for routine
checking for biologist or medicine personal. There is a high resolution
bridge and a standart goniometer available. We will be able to offer this
microsope on a negotiational base. Transfer of the equipment must be
handled by the buyer. For futher details contact me through email or by
fax.
DEADLINE is 31.01.96

Andreas Loewe


Guten Tag Freunde der Elektronenmikroskopie,

in unserer Abteilung ist ein Phillips EM300 aus dem Jahr 1971 zu verkaufen.
Das Ger=E4t wurde w=E4hrend der gesamten Zeit durch einen Wartungsvertrag
gepflegt und ist in sehr gutem Zustand. Dieses Mikroskop ist ideal f=FCr
Routinearbeiten von Biologen und Medizinern. Es ist sowohl mit einer
Hochaufl=F6sungsplattform als auch mit dem normalen Goniometer ausgestattet.
Transport und die Transportkosten =FCbernimmt der K=E4ufer. Preis ist
Verhandlungssache. F=FCr weitere Informationen schreiben Sie mich bitte unte=
r
meiner email-Adresse oder per Fax an.
EINSENDESCHLUSS f=FCr Interessenten ist der 31.01.96


Andreas Loewe

______________________________________________________________
Andreas Loewe Tel: +49-228-550-355
University of Bonn Fax: +49-228-678-413
Insitute for Inorganic Chemistry email: loewe-at-uni-bonn.de
Inorganic Material Research
Roemerstr. 164
53117 Bonn
Germany http://www.elmi.uni-bonn.de/
______________________________________________________________






From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Tue, 9 Jan 1996 09:11:32 -0500
Subject: Re: Image transfer and re-use

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Message-Id: {v01520d01ad18200e08bf-at-[155.37.2.10]}
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Hi Norman,

I can only emphasize what Bill Oliver wrote: Start with the basic question
and ask at what precision you want to set up your digital lab. The
acquisition precision (contrast resolution) of most microscopes (except
some operation modes for confocal, see Jim Pawley's recent remarks at "RE:
Flatbed scanners: 12-bit versus 8-bit") is far more than 8-bit. Most
hardware and new software is already available for 12-16 bit graytone and
color (per channel). If you plan for the future, start with 16-bit
uncompressed raw data handling. Every thing else may change monthly. You
may start using what is available now for handling } 8-bit data and expand
as new software and hardware becomes available. And don't spend much money
for a "good" printer, a HP LaserJet is just fine when expanded with one of
the available high resolution boards. I started to put many comparative
image data from my own experience in designing a "Precision Digital Imaging
Laboratory" in a WWW page at http://panda.uchc.edu/htklaus/index.html. May
be this is of help to you. Best regards Klaus




******************************************************************************
* : *
* Klaus-Ruediger Peters, Ph.D. : WWW Home Page: *
* Director, Molecular Imaging Laboratgory : *
* Biomolecular Structure Analysis Center : Molecular Imaging Laboratory *
* University of Connecticut Health Center : http://panda.uchc.edu/ *
* 263 Farmington Ave. : htklaus/index.html *
* Farmington, CT 06030-2017; U.S.A : Differential Hysteresis *
* : Processing Demo at http:// *
* Tel: (203) 679-3977; Fax: (203) 679-1989 : panda.uchc.edu./htbit/indiv/ *
* e-mail: Peters-at-BSAC.UCHC.EDU : /software_docs/dhp.html *
* : *
****************************************************************************
**






From: Owen P. Mills :      opmills-at-mtu.edu
Date: Tue, 9 Jan 1996 09:42:35 -0500
Subject: Increased LN2 usage of EDS detector?

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Regarding Randy Nessler's question about a valve to interface with the
vacuum jacket of an EDS detector.

I have an old valve that is manufactured by Cryolab, 159 Santa Fe Rd., San
Luis Obispo, CA. This is an old valve and the company may be gone/moved. I
have no connection with this company whatsoever.

I too have a detector with high LN2 consumption and am considering doing the
job myself. I assume that one would need the appropraite valve, a high
vacuum station, leak detector and bakeout blank/tape. Is anyone familiar
with the procedure for regenerating the EDS cyrostat?

Thanks,
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: images1-at-biosci.mbp.missouri.edu (Michael Stanley)
Date: Tue, 9 Jan 1996 11:51:34 -0600
Subject: tektronix 340+

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Hello All,

We are thinking about a new printer and are looking at the Tektronix 340+
Phaser. The quote that we received lists costs associated with printing as
"20% fill of color is $0.13 per page". Does anyone know the actual costs
per page? Is this the maximum if "1% of color is $0.01 per page"? How
much does it vary with size/color of image? What does this really mean?
Anyone tried this machine yet?

Any/all help will be greatly appreciated. TIA.

C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia, MO. 65211
(314) 882-4895
fax= 314-882-0123






From: oliver-at-ipas4.afip.mil (William R Oliver)
Date: Tue, 9 Jan 1996 13:46:19 -0500
Subject: Where to get free file conversion routines

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After my reply about file formats, I got a bunch of requests
about this mysterious pbmplus/netpbm library routine package.

Here's the story:

Way back when, there was this truly great dude by the name of
Jef Poskanzer, who decided to write a little library to
convert between formats. He decided to create his own
simple, easily editable and readable ascii format for
greyscale, binary, and color images. The idea was to
concentrate on making the format simple to poke around
in rather than being space-efficient since any image would
be in the simple format only "in transit". This would make
it easier for folk to write specific routines to convert their
pet formats to and from these simple forms rather than
worrying about trying to figure out every possible combination.

These simple formats were called Portable GreyMap, Portable
BitMap,and Portable PixMap images respectively, or pgm, pbm, and
ppm images. He and his buddies then wrote a ton of routines to
go to and from these simple formats. Thus, for instance, to
convert from GIF to TIFF, one would go from GIF to ppm using the
routine giftoppm, and then from ppm to TIFF by the routine ppmtotiff.

A number of simple manipulation routines were
added (rotation, scaling, smoothing, etc.) as well as some
other sophisticated and useful things. In addition, a number
of routines attempted to make the distinction between pgm, ppm,
and pbm transparent, and rolled them into the "Portable aNyMap"
or pnm format. Thus, one could also go from GIF to TIFF by
using giftopnm and pnmtotiff routines without having to
know if the image was greyscale or palette-based color. The pnm
format wasn't really a new format, but was used to denote those
routines which could accept pgm, pbm, and ppm format images and figure out
which "real" conversion routine to call.

This work of public service, called the "pbmplus" library
became too much of a time sink, and Jef Pokanzer stopped
putting out updates in 1991. It nonetheless represents a
public service effort right up there with GNU and the Fish disks.
All Hail these folk.

After this work, a number of other community-minded authors
added newer formats and other routines to the library. Since
these sometimes did not conform to Pokanzer's structural and
stylistic conventions and verification requirements, they
represented a superset over the pbmplus library rather than
an extension of it. This was labeled the netpbm library.
Thus, new stuff like SGI formats, are found in the netpbm
libraries, but not the pbmplus libraries.

I am including the beginning of the README file from the last
distribution I have installed on my system. I am sure that
things have been added since.

There are some formats, particularly the vector-based things,
that are not handled. If you are using Wavefront, 3DS, DXF,
and other CAD-type formats, you might want to look elsewhere.
One such place is the old Avalon site. The Avalon site was
a repository of 3D format convertors, models, etc. for 3D and
VR software. This site, originally maintained at a US Government
military base, was discontinued and was picked up by Viewpoint,
a company which makes 3D model datasets. It can be found at
http://www.viewpoint.com. Viewpoint has gotten mixed reviews for
its stewardship; some have attempted to set up alternate
repositories and others have applauded Viewpoint's effort if
not it's accomplishment. I neither endorse nor criticise Viewpoint or
the Viewpoint site, but simply state that it is still currently
the most complete site for finding public domain 3D format
conversion routines. If you are interested in the alternate sites,
I suggest you look at the 3D news groups and mailing lists. It
is a common topic of conversation on the Autodesk 3D studio
newsgroup and list.

Sites for these and other routines can also be found in the
FAQs for the graphics newsgroups.


One final note: Some of these routines are in turn dependent
on other library distributions. For isntance, the tiff routines
are dependent on the libtiff libraries which are a separate
distribution. A libtiff library *is* included in the standard
netpbm distribution, but the libtiff development history is
rather independent. Thus, you will want to keep these
libraries current if you want to be able to read and write
newer flavors of tiff.

Now, here's the start of the netpbm README, which gives
the sites as of early 1994 for the packages:

This README is dated 1993, but was current as of MARCH 1994.


N E T P B M
Release 7 December 1993

Netpbm is a toolkit for conversion of images between a variety of
different formats, as well as to allow a few basic image operations.
The package is intended to be portable to many platforms. It has been
tested under UNIX (BSD and SYSV, e.g. SGI, Sun4, Sun386i, DEC and
Apollo DN 3500), VMS and Amiga OS. There are also compiler directives
in it for MS-DOS.

You'll find the latest release of Netpbm at the following sites:
* wuarchive.wustl.edu (128.252.135.4),
directory /graphics/graphics/packages/NetPBM
* ikaros.fysik4.kth.se (130.237.35.2), directory /pub/netpbm.
* ftp.informatik.uni-oldenburg.de (134.106.1.9). This site also carries
binaries for the Amiga.
* peipa.essex.ac.uk (155.245.115.161), directory ipa/src/manip
* ftp.rahul.net (192.160.13.1), directory /pub/davidsen/source
* ftp.cs.ubc.ca, directory /ftp/archive/netpbm

You'll also find a mirror site at the BBS:
* sixhub.tmr.com, phone +1 518 3468033, in the "source" area.


Hope you find this useful.

billo

Any opinions are mine, and not necessarily those of Uncle Sam, or any
of his kith, kin, minions, or functionaries.






From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Tue, 9 Jan 1996 12:22:31 -0500
Subject: Re: Image transfer and re-use

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Hi Bill,

you recently replyed on the MSA-Listserver

that format is almost
} irrelevant, except for a couple of points. The reason for this is
} that there are numerous utilities for moving between formats, both
} public domain (such as the pbmplus and netpbm libs) and commercial.


} 1) Make sure you can save your images with enough depth. I generally
} use 24-, 32- and 48-bit images. Thus, formats which do not support
} at least 24 bit "truecolor" representation are useless for me.

and relatively few formats will easily allow
} you go move to 32-bits (Targa supports it well).
}
} 2) Make sure you use lossless compression

Thanks, that you came forward and stressed the same points I try to emphasis.


A quick question to you: I started to do my digtal DH imaging on a Silicon
Graphics workstation and of cause there is Tara File Format at home.
However, most of my PC and Mac based image acquisition is still in
MSA-Standard TIFF. You mentioned free software for file conversion. Could
you please provide a source for me?


Thanks, best regards Klaus.



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******************************************************************************
* : *
* Klaus-Ruediger Peters, Ph.D. : WWW Home Page: *
* Director, Molecular Imaging Laboratgory : *
* Biomolecular Structure Analysis Center : Molecular Imaging Laboratory *
* University of Connecticut Health Center : http://panda.uchc.edu/ *
* 263 Farmington Ave. : htklaus/index.html *
* Farmington, CT 06030-2017; U.S.A : Differential Hysteresis *
* : Processing Demo at http:// *
* Tel: (203) 679-3977; Fax: (203) 679-1989 : panda.uchc.edu./htbit/indiv/ *
* e-mail: Peters-at-BSAC.UCHC.EDU : /software_docs/dhp.html *
* : *
****************************************************************************
**



--============_-1390914345==_============--





From: James S MArtin :      James.S.Martin-at-williams.edu
Date: Tue, 9 Jan 1996 15:38:39 -0500 (EST)
Subject: Epi-fluorescence attachments for PLMs

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I'm preparing a presentation on polarized light microscopes that are
equipped for epi-fluorescence illumination, and need information from
microscope manufacturers regarding instruments purchased thus equipped
and retro-fitted with vertical fluorescence attachments.

I'm assuming that microscope manufacturers monitor this list. Please
reply directly to me. Thanks.

James Martin
Director of Analytical Services and Research
Williamstown Art Conservation Center
Williamstown, MA




From: bsgphy3-at-uconnvm.uconn.edu (JIM ROMANOW)
Date: Tue, 9 Jan 1996 17:40:09 -0500
Subject: Peldri

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We have used critical point drying for many years in our laboratory to dry
biological samples for SEM. Recently I decided to purchase some Peldri II
to test as an alternative to critical point drying, however when I
contacted Ted Pella they informed me that they are no longer selling it.
Instead they are selling two substitutes: tetramethylsilane and
hexamethyldisilazane. Does anyone have any experience with these yet? How
do they compare to Peldri and critical point drying? Why was Peldri
discontinued?

Jim Romanow
Electron Microscopy Facility
Physiology and Neurobiogy Department
The University Of Connecticut
Storrs
bsgphy3-at-uconnvm.uconn.edu






From: Andy Gilicinski :      giliciag-at-ttown.apci.com
Date: Tue, 9 Jan 1996 18:28:11 -0500 (EST)
Subject: Workshop: Industrial Applications of SPM

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______________________________________________
Preliminary Announcement:
THIRD WORKSHOP ON INDUSTRIAL APPLICATIONS OF
SCANNED PROBE MICROSCOPY
May 2-3 1996
NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY
Gaithersburg MD 20899
______________________________________________

A third Workshop on Industrial Applications of Scanned Probe
Microscopy (IASPM) will be held at NIST on May 2-3 1996.
The purpose is to continue exploring SPM standardization
and development needs anticipated for the next decade, with
a focus on industrial SPM users and hurdles that limit
broader use of SPM for industrial problem solving.

For more information, contact:
Dr. John Dagata
National Insitute of Standards and Technology
220-A117
Gaithersburg MD 20899
301-975-3597 voice
301-869-0822 fax
john.dagata-at-nist.gov






From: Gargent-at-aol.com
Date: Tue, 9 Jan 1996 18:13:32 -0500
Subject: Image Analysis

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Message-ID: {n1390904714.68273-at-mse.engin.umich.edu}

Are there any EM people heavy into Image processing and analysis. Currently
I am using a Kontron IBAS (version 2.0) and a KS400 Image analysis system.
Soon we will be incorporating a Telepathology system for communications
between the US, Switzerland, and Japan. Current Image imput for our routine
analysis come from EM micrographs, stored images, Kodak digital camera, from
the light microscope, and any source that allows us to capture, store, and
process images. We have several video camera, including a Sony 960 color
camera, B&W camera. We currently use PAL and NTSC formats.

I would be interested in knowing how people use computerized Image Analysis
for Stereology and Morphometry. Would be interested in knowing how to get
around point counting techniques with Electron Micrographs.

I would be willing to share IBAS macros with interested parties.
Gregory Argentieri
Sandoz Electron Microscopy lab
East Hanover, NJ 07936
201-503-8617
Greg2NJ-at-AOL.COM



Would welcome interested parties to exchange macros, ideas, image processing
tips etc.




From: Gargent-at-aol.com
Date: Tue, 9 Jan 1996 18:13:32 -0500
Subject: Image Analysis

Contents Retrieved from Microscopy Listserver Archives
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Are there any EM people heavy into Image processing and analysis. Currently
I am using a Kontron IBAS (version 2.0) and a KS400 Image analysis system.
Soon we will be incorporating a Telepathology system for communications
between the US, Switzerland, and Japan. Current Image imput for our routine
analysis come from EM micrographs, stored images, Kodak digital camera, from
the light microscope, and any source that allows us to capture, store, and
process images. We have several video camera, including a Sony 960 color
camera, B&W camera. We currently use PAL and NTSC formats.

I would be interested in knowing how people use computerized Image Analysis
for Stereology and Morphometry. Would be interested in knowing how to get
around point counting techniques with Electron Micrographs.

I would be willing to share IBAS macros with interested parties.
Gregory Argentieri
Sandoz Electron Microscopy lab
East Hanover, NJ 07936
201-503-8617
Greg2NJ-at-AOL.COM



Would welcome interested parties to exchange macros, ideas, image processing
tips etc.




From: Tony Bruton :      bruton-at-emu.unp.ac.za (by way of zaluzec-at-Microscopy.Com (Nestor J. Zaluzec))
Date: Wed, 10 Jan 1996 08:02:48 -0600
Subject: Old TEM available

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G'day world

We have a Hitachi HU-11E (~1968) which has to make way for new
developments. It was in regular use until about 2 years ago, now needs
reference batteries and a kick-start. If anyone has a use for spares or
the complete unit then please make contact with us ASAP. No
reasonable offer refused !! It has to be cleared by March.

Tony Bruton
Electron Microscope Unit
University of Natal
Pietermaritzburg
Kwa-Zulu Natal
South Africa
Phone 0331 2605155 or fax 0331 2605776








From: davilla-at-4pi.com (Scott D. Davilla)
Date: Wed, 10 Jan 1996 09:38:10 -0600
Subject: Increased LN2 usage of EDS detector?

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Message-Id: {199601101419.GAA08413-at-holonet.net}

If you are in need of vaccum pumping of an EDS detector, you might
give contact;

Doug Conners
TN Analyzer Service
608-798-2005

Scott D. Davilla Phone: 919 489-1757 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707







From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Wed, 10 Jan 1996 11:00:58 -0600
Subject: Re: "Antique" microscopes

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Message-Id: {199601101604.KAA28262-at-BCM.TMC.EDU}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Bob -

There is, or was, a wonderful shop called Historical Technology in
Marblehead, Mass. that buys and sells old microscopes as well as other
pieces of technology. I haven't delt with them in a long time, and they may
no longer be in business. But it would be worth the effort to contact them
just to get one of their catalogs. They are:

Historical Technology
6 Mugford St.
Marblehead, Massachusetts 01945
(617) 631-2275

Sorry that this reply is so late, but I had to go mining in a closet to find
the catalog.

Joiner
************************************

At 02:07 PM 1/4/96 +0000, you wrote:
} Our department has about a dozen old Spencer light microscopes--monoccular,
} black laquer with lots of brass, probably pre-WWII. Does anyone know if
} there is a market for such things out there? Are there 'antique'
} microscope dealers who might be interested in these? They are not very
} functional but too nice to throw away.
}
} Bob
}
}
} Robert R. Wise, PhD
} Department of Biology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} (414) 424-3404 tel
} (414) 424-1101 fax
} wise-at-vaxa.cis.uwosh.edu
}
}
}
}





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Wed, 10 Jan 1996 11:58:36 -0600
Subject: Re: Peldri

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X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
Message-Id: {v02130501ad19ad3d832e-at-[131.230.97.68]}
Mime-Version: 1.0
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Jim Romanow asked:
} We have used critical point drying for many years in our laboratory to dry
} biological samples for SEM. Recently I decided to purchase some Peldri II
} to test as an alternative to critical point drying, however when I
} contacted Ted Pella they informed me that they are no longer selling it.
} Instead they are selling two substitutes: tetramethylsilane and
} hexamethyldisilazane. Does anyone have any experience with these yet? How
} do they compare to Peldri and critical point drying? Why was Peldri
} discontinued?
}

Peldri II was dropped because it is a Freon - restrictions are becoming
tighter - and because Pelco is an environmentally conscious company (I have
no financial or emotional connection to Pelco). In our facility, we have
used HMDS and it gives similar results as Peldri and CPD. Since your
results will be highly dependent upon the type of specimen and processing
(fixation, dehydration, etc) it is best to run a comparative trial using
HMDS, TMS and CPD. I suspect that HMDS will be fine.
Contact me if you need more info.

#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 10 Jan 1996 12:20:35 -0500
Subject: EM Fixative

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Message-Id: {n1390845623.57279-at-msmail.tmc.tulane.edu}

I posted this some time ago and got not a single response. Someone here
approached me about a fixative called K11 that I never used. Please let me
know what you know about it or give a reference for starter. Thanks.
******************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} Prof. Pathology & Otolaryngology *
* http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html *
******************************************************************






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Wed, 10 Jan 1996 09:10:08 GMT
Subject: Re: Peldri

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} We have used critical point drying for many years in our laboratory to dry
} biological samples for SEM. Recently I decided to purchase some Peldri II
} to test as an alternative to critical point drying, however when I
} contacted Ted Pella they informed me that they are no longer selling it.
} Instead they are selling two substitutes: tetramethylsilane and
} hexamethyldisilazane. Does anyone have any experience with these yet? How
} do they compare to Peldri and critical point drying? Why was Peldri
} discontinued?
}
} Jim Romanow
} Electron Microscopy Facility
} Physiology and Neurobiogy Department
} The University Of Connecticut
} Storrs
} bsgphy3-at-uconnvm.uconn.edu
}
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

We frequently use HMDS instead of critical point(CPD) It does not work well
on all samples. Plants generally require CPD. Many microbes, insects,
cells cultures and animal tissues can be successfully dried via HMDS. We
only briefly tested Peldri and were not impressed. It showed no advantage
over HMDS and was more tedious to use. We have not tried the
Tetramethylsilane, but I suspect it would perform much the same as HMDS.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Wed, 10 Jan 1996 09:10:08 GMT
Subject: Re: Peldri

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} We have used critical point drying for many years in our laboratory to dry
} biological samples for SEM. Recently I decided to purchase some Peldri II
} to test as an alternative to critical point drying, however when I
} contacted Ted Pella they informed me that they are no longer selling it.
} Instead they are selling two substitutes: tetramethylsilane and
} hexamethyldisilazane. Does anyone have any experience with these yet? How
} do they compare to Peldri and critical point drying? Why was Peldri
} discontinued?
}
} Jim Romanow
} Electron Microscopy Facility
} Physiology and Neurobiogy Department
} The University Of Connecticut
} Storrs
} bsgphy3-at-uconnvm.uconn.edu
}
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

We frequently use HMDS instead of critical point(CPD) It does not work well
on all samples. Plants generally require CPD. Many microbes, insects,
cells cultures and animal tissues can be successfully dried via HMDS. We
only briefly tested Peldri and were not impressed. It showed no advantage
over HMDS and was more tedious to use. We have not tried the
Tetramethylsilane, but I suspect it would perform much the same as HMDS.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 10 Jan 1996 11:08:58 -0500 (EST)
Subject: Re: tissue water content

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} A colleague needs to accurately determine the water content of very small
} tissue samples (fish embryos at various stages of development). What would
} be the best way to do this? Thanks for any suggestions. Grace

Dear Grace,
Can your colleague measure the weights and/or volumes of both the
hydrated and lyophylized embryos? ESEM could give you the hydrated volumes,
and coating the residue and using SEM might be adequate for the dehydrated
volume as long as the residue packs with no voids. It might be easier simply
to weigh both specimens. I realize, of course, that volatile salts will be
lost during lyophylization, so that may not give accurate enough results.
On another tack, is there a measure--such as osmotic activity--
which could be used? If so, one could put the embryos in a solution of PEG
or some other macromolecular or impermient solute and note the concentration
at which the embryos neither swell nor shrink. That might give enough infor-
mation to determine the water content if there are a series of tissue samples
of differing contents to use as standards. Good luck.
Yours,
Bill Tivol




From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Wed, 10 Jan 1996 20:16:52 +0000 (GMT)
Subject: Re: SCANNING 96

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Message-ID: {199601101405.JAA22696-at-IndyNet.indy.net}
To: SPM List {spm-at-di.com} , Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

Dear Lynn:

I got your round robin e-mail about Scanning 96. I am submitting two
papers but find there is an ambiguity in the instructions for preparing
the abstracts.
Please advise ASAP tp pe13-at-cus.cam.ac.uk whether the abstract should be
only about 750 words long OR whether I can write an abstract which nearly
fills the two pages on the Instructions to Authors. I would prefer the
latter as I have a lot I want to cram in. Rest assured the two abstracts
will be wih you by the end of the month OR when you have dug your self
out of the snow drifts

Best wishes

Patrick Echlin

On Wed, 10 Jan 1996, Lynn Savino wrote:

} To all those interested in participating in SCANNING 96
}
} Deadline for abstracts is February 1, 1996.
}




From: Stephanie Evans :      stevans-at-bgsm.edu
Date: Wed, 10 Jan 1996 15:31:30 -0500 (EST)
Subject: Re: Peldri

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On Tue, 9 Jan 1996, JIM ROMANOW wrote:

} We have used critical point drying for many years in our laboratory to dry
} biological samples for SEM. Recently I decided to purchase some Peldri II
} to test as an alternative to critical point drying, however when I
} contacted Ted Pella they informed me that they are no longer selling it.
} Instead they are selling two substitutes: tetramethylsilane and
} hexamethyldisilazane. Does anyone have any experience with these yet? How
} do they compare to Peldri and critical point drying? Why was Peldri
} discontinued?
}
} Jim Romanow
} Electron Microscopy Facility
} Physiology and Neurobiogy Department
} The University Of Connecticut
} Storrs
} bsgphy3-at-uconnvm.uconn.edu
}
}
}
Jim,
We have used HMDS(hexamethyldisilazane) in the past with good result. I
still like to CPD the non-repeateble samples but have found HMDS to be
suitable for most samples.
Hope this helps



-------------
Stephanie Evans
WFU/BGSM
W-S,N.C.27157









From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 10 Jan 1996 16:54:43 -0500 (EST)
Subject: Re: Increased LN2 usage of EDS detector?

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} It seems that one of our EDS detectors consumption of LN2 has
} increased as of late. I am making the assumption that possibly the vacuum
} needs regeneration in the dewar. Does anyone know where one might acquire
} the fitting to interface a vacuum system to the dewar of a Kevex detector?
} Thank you in advance,

Dear Randy,
When this occurred with our Kevex, after a few times sending the
whole thing back for regeneration, I had our shop make a back plate with
a 1/2" copper tube attached. A swagelok fitting and valve allowed me to
attach to the column vacuum, and I've been able to use that for the rege-
neration ever since. The resolution is still reasonable after } 10 years--
155 ev, as I remember--so this method works for us. If you have a very
clean column vacuum, you can attach a turbo or ion pump (possibly a cold-
trapped DP will do, but ask Kevex) to the tubing and not foul up the column.
~10^-6 or so will regenerate the cryosorbant. Good luck.
Yours,
Bill Tivol




From: HAHNP-at-JEFLIN.TJU.EDU
Date: Thu, 11 Jan 1996 0:50:46 -0500 (EST)
Subject: color image processing

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hello fellow digital microscopists.
from snow bound Philly comes a query for advice on color image processing of
images. does anyone use a package that can quantify/image process color data
instead of just greyscale?


please post replies directy and I will share summary with all.



._____________________
| Peter J. Hahn
| -------------
| Thomas Jefferson University
3401 Spring Garden Street
Philadelphia, PA. 19104
tel : 215-349-0099 |
fax : 610-356-3451 |
peter.hahn-at-mail.tju.edu |
-------------+-----------------+----+------+





From: dremsen-at-mbl.edu (David Remsen)
Date: Thu, 11 Jan 1996 10:05:21 -0500
Subject: COURSE ANNOUCEMENT: Cell and Molecular Imaging Using Cryotechniques

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Message-Id: {v01530505ad1ad69e913c-at-[128.128.172.251]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

The Marine Biological Laboratory in Woods Hole, MA has announced the
following course. More information and applications can be obtained at
http://www.mbl.edu/html/guide/guide.home.html or by writing admissions-at-mbl.edu


Cell and Molecular Imaging Using Cryotechniques

Courses Dates: May 28-June 5, 1996

Course Description: An eight-day comprehensive course/workshop on
cryotechniques for cell and molecular imaging and analysis. Lecture and
special discussion sessions in the mornings and hands-on lab sessions in
the afternoons and evenings will form the heart of the course. Limited to
24 participants.

Topics will include freezing methods (including high pressure freezing),
freeze-substitution, cryosectioning, immunogold localization,
freeze-fracture, Low Temperature SEM, cryo microscopy (TEM), image
processing and X-ray microanalysis. Lectures and labs will be conducted by
experts in the field from universities and industry.

Attendees will be expected to have a basic knowledge of electron
microscopy. The class will attend common lecture sessions and will divide
into groups for lab sessions and discussions on special topics. Attendees
will be encouraged to consult with the course director about bringing their
own specimens for use during lab sessions.

Director: M.V. Parthasarathy, Cornell University.

Faculty: Frank Booy, National Institutes of Health; Patrick Echlin,
Cambridge University, UK; Kent McDonald, University of California,
Berkeley; Martin Mueller, ETH, Zurich, Switzerland; Klaus-Ruediger Peters,
University of Connecticut Health Center; E. B. Prestridge, Princeton
Gamma-Tech, Inc.; John Rash, Colorado State University; Daniel Studer,
University of Bern, Switzerland; William Wergin, ARS-USDA; Karl Zierold,
Max-Planck Institute for Molecular Physiology, Germany; and others to be
named.


=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
David Remsen
Marine Biological Laboratory






From: dremsen-at-mbl.edu (David Remsen)
Date: Thu, 11 Jan 1996 10:40:24 -0500
Subject: URL revision for COURSE ANNOUNCEMENT

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Message-Id: {v01530508ad1adf5d9f49-at-[128.128.172.251]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

To my chagrin I have posted an incorrect URL for more information on the
Cryotechniques course at the Marine Biological Laboratory.

The correct URL is

http://www.mbl.edu/html/GUIDE/guide.home.html

Thank you,


=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
David Remsen
Marine Biological Laboratory






From: William R Oliver :      oliver-at-ipas4.afip.mil
Date: Thu, 11 Jan 1996 12:20:51 -0500 (EST)
Subject: Re: color image processing

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Once again, my opinions only:


You need to be a bit more specific. When some folk talk about
"image processing," they mean the kind of things you do to get
an image ready for presentation or publication -- annotation,
a little contrast buffing, etc. Other folk mean playing with
point spread functions and such for image regularization. Still
others mean doing image manipulation for data extraction -- segmentation,
object recognition, metrics of various sorts, etc. Finally, 2D and 3D
products are rather different.

The type of package you might want depends upon the specific task and
the platform you like. For instance, in my work with forensic image
processing, I use AVS (Advanced Visualization Systems) as a programming
environment, and incorporate libraries and code from a number of
academic insititutions (for instance, DIAL and /usr/image from
University of North Carolina and the University of Utrecht). AVS, and
its big brother AVS Express, use a dataflow paradigm which takes the UNIX
pipe to extremes. In this paradigm, you program little modules, say fft,
and then plunk an icon for that module on the screen. You then connect
other modules to it (say, a "read image" module) using little pipes.
You can then build up larger applications by linking these little modules
in networks. AVS comes with a set of supported modules, and there is a
large set of community-donated public domain modules.

This is specific to UNIX-based systems, however. Most specific platforms
have their own image processing libraries that you can buy. I know that
Sun has something, SunVision, I think, and SGI also has its image
processing toolbox. SGI also incorporates some image processing into its
Explorer package, which uses the same kind of dataflow paradigm as AVS.

Excellent public domain Unix image processing packages which include
advanced routines include Khoros from U. New Mexico, and IRAF. Khoros is
both a toolbox and/or development environment which incorporates a
dataflow paradigm much like AVS and Explorer. The AVS-like front end for
Khoros is called Cantata. IRAF, which is the common platform for academic
astronomical image processing, has some very nice tools for deconvolution
and image restoration, but it is geared heavily towards astronomical models
and is a true bear to install.

NIH has a package called, I think, NIH Image, but I have never used it. I
have heard good things, but I think it is specific to Macs, is for
greyscale only, and is 8-bit only.

For some of the cellular morphometric stuff, we tend to use PC-based
software such as Optimas. There are, however, two or three other
relatively good systems for doing metrics and more advanced image
processing such as ImagePro and there's this French program by Noesis
called, I think, Visilog. Each of these have routines for most common
simple, intermediate and advanced image processing and 2D measurement
tasks. I like the programming interface for Optimas. Visilog has, as
far as I can see, a larger set of ready-to-use tools for mathematical
morphologic manipulation, which you would expect from the French, I
suppose. All of these systems allow, in one way or another, incorporation
of home-brewed routines either as a link to compiled code or by using a
macro language.

For annotation and aesthetic stuff, I prefer Picture Publisher by
Micrografx. Many like Photoshop, and I have used it occasionally, but I
still prefer Picture Publisher. Photostyler (Aldus) and Corel are also
good products. I find Picture Publisher to be more intuitive and easier
to use. It has a harder time successfully importing images, however. Our
PCs are on a network with Unix boxes, and the images get to the PCs via
nfs. My installation of Picture Publisher has problems often with reading
large images over the network and with decoding large or compressed
images. I often read an image from the network in using Photostyler, save
it locally, and then manipulate it using Picture Publisher. I have found
the Corel product a memory hog, and gets really slow with big (40 Mbyte)
images.

Aside from the memory and importing hassles, the biggest differences with
these artistic manipulators from that point on involve specific tools --
how you want to set brush sizes, how you want to play with overlays, how
the gui is set up (whether you prefer type-in widgets, etc.). For
instance, Picture Publisher has a slider transparency control on a toolbar
for interactively playing with overlays, and allows you to play with
overlay parameter during a paste. Other programs require multilevel menu
selection to set transparency, which is not as easy. The (rather old)
version of Photostyler I have used has a pretty primitive file requestor
for loading images. Picture Publisher has a requestor that does
thumbprints. This is a mixed blessing with a heterogeneous network -- it
makes browsing easier, but the thumbprints can eat up disk space, they can
"get lost" when the unix directories get mapped to DOS names, etc. Which
set of widgets and approaches is most intuitive or easiest to learn is a
matter of personal taste. Fractal Paint and associated tools have really
nice interfaces for doing true "artistic" manipulation in the sense of
having results that look like true artistic media (charcoal, oils, etc.)
and "natural" textures. There is another package called {something - I
forget} Matisse, which also attempts the "true" artistic approach. The
{something} Matisse product, however, can get really slow with larger images.
I have found it useless for work but a gas to play with.

There are a number of public domain systems which do limited image
manipulation -- scaling, rotating, cropping, etc. Some of these have
their own little areas of surprise. I have already discussed the
pbmplus/netpbm libraries. There are others, such as ImageMagick, Utah
Raster Toolkit, etc. which provide similar routines.

For integration of images into presentations and some buffing for
publication (cropping, playing with backgrounds, etc.) I use
Corel, though many also like Harvard Graphics. I just happened to have
Corel at home, and never wanted to bother with the learning curve for
Harvard Graphics or PowerPoint. My wife uses Harvard Graphics and I have
helped her on occasion -- it is fairly intuitive.

For 3D modeling work, I have worked with AutoCAD, 3d Studio, and Lightwave
3D. Each has specific strengths and weaknesses, but I bet you aren't
interested in these, so I will spare you reviews. For high-end production
on UNIX boxes, there are Wavefront, Alias, SoftImage, and ElectroGig. I
have worked with Wavefront and Gig, if you want reviews.

Finally, there are specific tools for such things as texture generation,
3D painting, titling, etc. For PCs, for instance, Kai's Power Tools is
an excellent product. For UNIX boxes, Xaos has recently released a
number of good tools.

3D visualization tools for medical images which incorporate some
measurement capability include some commercial products which have been
discussed ad-nauseum on the confocal list, so I won't discuss them here.
There is a pseudo-commercial product out of Mayo called Analyze which is
primarily a 3D visualization tool with some measurement capability. Last I
heard, Rich Robb was charging about 5000 bucks for it. There is a
relatively cheap tool from UPenn (Jay Udupa's lab) called 3DViewnix which
runs about a thousand bucks which again is primarily a visualization tool
with some measurement capability. There is a free tool out of Arie
Kaufmann's lab at SUNY called VolVis which is, again, primarily a
visualization tool with some measurement capability. I have heard good
things about a program called Confocal Assistant, but have never seen or
tried it. I believe it is freely distributable, but I am not sure.


hope this helps,

billo


All opinions are my own and do not necessarily reflect those of the US
Govt, Army, Uncle Sam, yada, yada, yada.

On Thu, 11 Jan 1996 HAHNP-at-JEFLIN.TJU.EDU wrote:

} hello fellow digital microscopists.
} from snow bound Philly comes a query for advice on color image processing of
} images. does anyone use a package that can quantify/image process color data
} instead of just greyscale?
}
}
} please post replies directy and I will share summary with all.
}
}
}
} ._____________________
} | Peter J. Hahn
} | -------------
} | Thomas Jefferson University
} 3401 Spring Garden Street
} Philadelphia, PA. 19104
} tel : 215-349-0099 |
} fax : 610-356-3451 |
} peter.hahn-at-mail.tju.edu |
} -------------+-----------------+----+------+
}
}




From: Annette M. Andrews :      AANDREWS-at-NCTR.FDA.GOV
Date: Thu, 11 Jan 1996 12:15:06 CDT
Subject: SEM Examination of Graphite

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Our facility has been requested to examine a series of artificial
heart valves made of high-tech graphite utilizing the SEM. We will
need to initially examine the valves prior to the first test to
establish a baseline of the surface. They will be returned to the
testing site for further testing for wear then returned to us for
re-examination. We will be unable to sputter coat the valves with
any type of conducting material.

I would like any suggestions for proper examination of graphite.
Since this is my first experinece with graphite, my first choice
would be to examine the valves at a low Kv (5, 10, or 15) and at a
long working distance of 39. My instrument is a JEOL JSM-35 SEM.

Any suggestions and/or comments may be forwarded to my direct e-mail
address: aandrews-at-fdant.nctr.fda.gov
Thank you.




From: Gary Login on Wed, Jan 10, 1996 3:46 PM
Date: 11 Jan 1996 12:16:41 -0500
Subject: Re: EM Fixative

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Message-Id: {n1390759410.38417-at-msmail.tmc.tulane.edu}

Thanks to all who responded.
_______________________________________________________________________________

Cesar, I use an aldehyde-based fixative called 'KII'. It is a dilute
Karnovsky's
fixative containing: 2% formaldehyde, 2.5% glutaraldehyde, 0.025% calcium
chloride in 0.1 M sodium cacodylate buffer, pH 7.4.

Gary Login


In message {n1390845623.57279-at-msmail.tmc.tulane.edu} "Fermin, Cesar" writes:
} I posted this some time ago and got not a single response. Someone here
} approached me about a fixative called K11 that I never used. Please let me
} know what you know about it or give a reference for starter. Thanks.
} ******************************************************************
} *Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
} *Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
} *Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
} *New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
} * {Fermin-at-tmc.tulane.edu} Prof. Pathology & Otolaryngology *
} * http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html
*
} ******************************************************************
}


Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676







From: Joyce Palmer, ECE Marcus 20 413-545-4647 :      PALMER-at-ecs.umass.edu
Date: Thu, 11 Jan 1996 15:50:48 -0500
Subject: Optical microscopes

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Dear Colleagues,
I recall recently people "advertizing" used optical microscopes on this
list. I didn't need one at the time, so I did not save the messages, but I now
find myself in need of a optical microscope. I specifically need one
that has a calibrated focus knob so that I can make thickness meaurements in
the 1-50 micron range by focusing on the front and back surfaces of our
(transparent) samples.
Please contact me if you know where I might find an inexpensive (used
is fine, especially if it's inexpensive) microscope for the above task.

Thanks in advance
Joyce Palmer




From: slakmon-at-scottscientific.com (P. Slakmon)
Date: Thu, 11 Jan 1996 18:25:11 -0500
Subject: sputter coater

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If anybody in Canada has a used sputter coater, no more than five year old
to sell, please send me details.


Thank you,

Philip Slakmon
____________________________________________________________________
Scott Scientific Telephone: (514) 485-2309
P.O. Box 66552, Station Cavendish Facsimile: (514) 485-9931
Montreal, Quebec Voice Mail:(514) 888-6509
H4W 3J6, Canada e-mail:
Slakmon-at-ScottScientific.com
URL:
http://www.ScottScientific.com
____________________________________________________________________





From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 11 Jan 1996 17:19:31 -0800
Subject: dye sub gray scale

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Message-Id: {199601120118.RAA21464-at-cats.ucsc.edu}
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Has anyone run into a problem printing grayscale images on a Kodak/Codonics
printer?

Ours have started looking tinged with green/yellow right out of the
printer. I have read that the formulation of the ribbons may have changed
and that the result is this sick green color.

I would like to avoid swapping color/grayscale ribbons if possible but my
users don't like the color cast to their pictures. Any ideas for a
solution?


Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
jmkrupp-at-cats.ucsc.edu






From: krogers-at-ecn.purdue.edu (Kirk Rogers)
Date: Thu, 11 Jan 1996 23:11:17 -0500
Subject: Re: Scanners

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I was leafing through Macworld the other day and realized that they had
reviewed several scanners in the last 6 months. The recommendes ones were
(4 or more stars out of 5):

Flatbed scanners:

HP Scanjet 3c
30 bit dynamic range, 600 dpi optical resolution, list $1179,
street ~$700
208-323-2551

PixelCraft Pro Imager 8000
30 bit, 1200 dpi optical, list $13k
510-562-2480


35 mm negative scanner:

Polaroid SprintScan 35
30 bit, interpolated 2700 dpi, list $2495, street ~$1600


-Kirk
_____________________________________________
Kirk Rogers krogers-at-materials.ecn.purdue.edu
OR
kirk.a.rogers.1-at-purdue.edu
Purdue University, School of Materials Science and Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289
OFFICE: 317-494-8751 FAX: 317-494-1204
http://materials.ecn.purdue.edu/~krogers






From: krogers-at-ecn.purdue.edu (Kirk Rogers)
Date: Thu, 11 Jan 1996 22:40:57 -0500
Subject: Re: color image processing

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Message-Id: {v01530501ad1b79f24a9f-at-[128.46.155.222]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} NIH has a package called, I think, NIH Image, but I have never used it. I
} have heard good things, but I think it is specific to Macs, is for
} greyscale only, and is 8-bit only.

I believe NIH Image supports 4, 8 and 16 bit TIFF (as well as PICT, pics,
macpaint) images in both color and grayscale and 24 bit color images.
Image will also open, edit, compare frames, average frames, etc. on
captured video or quicktime movies. NIH Image now (v. 1.59) includes
several built-in fast fourier transforms, although these run rather slowly
on 68k Macintoshes. And heck, it's free!!

{ftp://zippy.nimh.nih.gov/pub/nih-image/}

The 8-bit part is in the way Image handles adjacent pixels. In other
words, all contiguous pixels are counted during operations, not just the 4
pixels (4-bit) that share a side with the pixel in question. In doing
microstructural analysis of some acicular structures we found our $50k
workstation-based image analysis system was not able to analyze the
microstructure as well as NIH Image because it was a 4-bit system.

For those interested in doing 2D image analysis and reconstruction, The
National Center for Supercomputing Applications (NCSA) at UIUC, has a
program called NCSA Image. I just visited their web site,

{http://www.ncsa.uiuc.edu/General/NCSAHome.html}

and found that Image is now called Collage, and is available for Macintosh
and X-Windows for FREE. Collage uses a proprietary data format, HDF, but
you can get Import2HDF, which will convert FITS, TIFF, GIFF, Pict, ascii
and rastor files to HDF. NCSA Collage also supports real time interaction
between scientists working on the same data sets at remote locations
according to the readme file.

NCSA GelReader, which automates the measurement of DNA length using
digitized electrophoretic gels is also available for free from NCSA, and is
only available for the Macintosh.

(Insert standard disclaimer about not being intimately involved with these
products here.)


-Kirk
_____________________________________________
Kirk Rogers krogers-at-materials.ecn.purdue.edu
OR
kirk.a.rogers.1-at-purdue.edu
Purdue University, School of Materials Science and Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289
OFFICE: 317-494-8751 FAX: 317-494-1204
http://materials.ecn.purdue.edu/~krogers






From: Nancy K. R. Smith :      SMITHN-at-UTHSCSA.EDU
Date: Thu, 11 Jan 1996 20:40:57 -0500 (CDT)
Subject: Free: Image Analysis Software for Windows95

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In response to recent discussion on this listserver regarding image
analysis, I am forwarding description of newly available, FREE image
analysis software, that was specifically designed to do for Windows users
what NIH Image does for Mac users, and much more. I recently saw a
demonstration of it and was impressed. Most impressive is that it is
available absolutely FREE. It can be downloaded on the web from the Dental
Diagnostic Science Dept of the University of Texas Health Science Center at
San Antonio (ddxds.uthscsa.edu). This program was released the same day as
Windows95 & has already been distributed worldwide.
(This is not a commercial message. Even the creators of the software are
not benefitting financially from its distribution).
Soon to be released is a portion of the program that will make possible
quantitation of the gels that molecular biologists love to run. =20
}
} Date: Thu, 11 Jan 1996 16:03:08 -0500 (CDT)
} From: "S. Brent Dove" {dove-at-uthscsa.edu}
} Subject: Re: Image Analysis
} To: "Nancy K.R. Smith" {smithn-at-uthscsa.edu}
}
} Hello Nancy,
}
} Here is a basic description of UTHSCSA ImageTool. Feel free to distribute
this to any colleagues in the microscopy world.
}
} UTHSCSA
} IMAGETOOL
}
} OVERVIEW
}
} UTHSCSA ImageTool (IT) is a free image processing and analysis program for
Microsoft Windows 95=81 or Windows NT=81. IT can acquire, display, edit,
analyze, process, compress, save and print gray scale and color images. IT
can read and write over 22 common file formats including BMP, PCX, TIF, GIF
and JPEG. Image analysis functions include dimensional (distance, angle,
perimeter, area) and gray scale measurements (point, line and area histogram
with statistics). ImageTool supports standard image processing functions
such as contrast manipulation, sharpening, smoothing, edge detection, median
filtering and spatial convolutions with user-defined convolution masks. IT
also has built-in macro capabilities that allow the user to record
repetitive tasks and playback saved macros to automate image analysis.
}
} ImageTool was designed with an open architecture that provides extensiblity
via a variety of plug-ins. Support for image acquisition using either Adobe
Photoshop plug-ins or Twain scanners is built-in. Custom analysis and
processing plug-ins can be developed using the software development kit
(SDK) provided (with source code). This approach makes it possible to solve
almost any data acquisition or analysis problem with IT.
}
} ImageTool provides for geometric transformations such as rotate, flip
vertical, flip horizontal and magnification up to four levels. All analysis
and processing functions are available at any magnification factor. The
program is a multiple document interface (MDI) application supporting any
number of windows (images) simultaneously. =20
}
} Spatial calibration is available to indicate real world dimensional
measurements such as millimeters, microns, feet, miles, etc. for linear and
area. Density or gray scale calibration can be done relative to radiation
or optical density (OD) standards.
}
} IT version 1.1 now provides for object analysis and classification with
over 20 morphological descriptors such as: area/perimeter, roundness, ferret
diameter, compactness, major/minor axis length, centroid and many others.
Any of these factors can be used automatically categorized and count objects
within the image.
}
} ImageTool ver 1.1 supports the Data Translation DT3155 frame grabber for
Windows NT. Other frame grabber board will be added in the coming months.=
=20
}
} ImageTool was written using Borland's C++ version 4.5 and the source code
for the executable is available free of charge. IT was developed in the
Department of Dental Diagnostic Science at The University of Texas Health
Science Center, San Antonio, Texas. The program was developed by C. Donald
Wilcox, S. Brent Dove, W. Doss McDavid and David B. Greer.
}
}
}
}
} S. Brent Dove Voice: (210) 567-3333
} Diagnostic Sciences Fax: (210) 567-3334
} University of Texas Email: dove-at-uthscsa.edu
} Health Science Center Web: ddxds.uthscsa.edu
} San Antonio, TX USA ftp: maxrad6.uthscsa.edu
}
}





From: zqliu-at-pccms.pku.edu.cn (zhenquan Liu)
Date: 11 Jan 1996 12:16:41 -0500
Subject: Re: color image processing

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HAHNP-at-JEFLIN.TJU.EDU
Once again, my opinions only:


You need to be a bit more specific. When some folk talk about
"image processing," they mean the kind of things you do to get
an image ready for presentation or publication -- annotation,
a little contrast buffing, etc. Other folk mean playing with
point spread functions and such for image regularization. Still
others mean doing image manipulation for data extraction -- segmentation,
object recognition, metrics of various sorts, etc. Finally, 2D and 3D
products are rather different.

The type of package you might want depends upon the specific task and
the platform you like. For instance, in my work with forensic image
processing, I use AVS (Advanced Visualization Systems) as a programming
environment, and incorporate libraries and code from a number of
academic insititutions (for instance, DIAL and /usr/image from
University of North Carolina and the University of Utrecht). AVS, and
its big brother AVS Express, use a dataflow paradigm which takes the UNIX
pipe to extremes. In this paradigm, you program little modules, say fft,
and then plunk an icon for that module on the screen. You then connect
other modules to it (say, a "read image" module) using little pipes.
You can then build up larger applications by linking these little modules
in networks. AVS comes with a set of supported modules, and there is a
large set of community-donated public domain modules.

This is specific to UNIX-based systems, however. Most specific platforms
have their own image processing libraries that you can buy. I know that
Sun has something, SunVision, I think, and SGI also has its image
processing toolbox. SGI also incorporates some image processing into its
Explorer package, which uses the same kind of dataflow paradigm as AVS.

Excellent public domain Unix image processing packages which include
advanced routines include Khoros from U. New Mexico, and IRAF. Khoros is
both a toolbox and/or development environment which incorporates a
dataflow paradigm much like AVS and Explorer. The AVS-like front end for
Khoros is called Cantata. IRAF, which is the common platform for academic
astronomical image processing, has some very nice tools for deconvolution
and image restoration, but it is geared heavily towards astronomical models
and is a true bear to install.

NIH has a package called, I think, NIH Image, but I have never used it. I
have heard good things, but I think it is specific to Macs, is for
greyscale only, and is 8-bit only.

For some of the cellular morphometric stuff, we tend to use PC-based
software such as Optimas. There are, however, two or three other
relatively good systems for doing metrics and more advanced image
processing such as ImagePro and there's this French program by Noesis
called, I think, Visilog. Each of these have routines for most common
simple, intermediate and advanced image processing and 2D measurement
tasks. I like the programming interface for Optimas. Visilog has, as
far as I can see, a larger set of ready-to-use tools for mathematical
morphologic manipulation, which you would expect from the French, I
suppose. All of these systems allow, in one way or another, incorporation
of home-brewed routines either as a link to compiled code or by using a
macro language.

For annotation and aesthetic stuff, I prefer Picture Publisher by
Micrografx. Many like Photoshop, and I have used it occasionally, but I
still prefer Picture Publisher. Photostyler (Aldus) and Corel are also
good products. I find Picture Publisher to be more intuitive and easier
to use. It has a harder time successfully importing images, however. Our
PCs are on a network with Unix boxes, and the images get to the PCs via
nfs. My installation of Picture Publisher has problems often with reading
large images over the network and with decoding large or compressed
images. I often read an image from the network in using Photostyler, save
it locally, and then manipulate it using Picture Publisher. I have found
the Corel product a memory hog, and gets really slow with big (40 Mbyte)
images.

Aside from the memory and importing hassles, the biggest differences with
these artistic manipulators from that point on involve specific tools --
how you want to set brush sizes, how you want to play with overlays, how
the gui is set up (whether you prefer type-in widgets, etc.). For
instance, Picture Publisher has a slider transparency control on a toolbar
for interactively playing with overlays, and allows you to play with
overlay parameter during a paste. Other programs require multilevel menu
selection to set transparency, which is not as easy. The (rather old)
version of Photostyler I have used has a pretty primitive file requestor
for loading images. Picture Publisher has a requestor that does
thumbprints. This is a mixed blessing with a heterogeneous network -- it
makes browsing easier, but the thumbprints can eat up disk space, they can
"get lost" when the unix directories get mapped to DOS names, etc. Which
set of widgets and approaches is most intuitive or easiest to learn is a
matter of personal taste. Fractal Paint and associated tools have really
nice interfaces for doing true "artistic" manipulation in the sense of
having results that look like true artistic media (charcoal, oils, etc.)
and "natural" textures. There is another package called {something - I
forget} Matisse, which also attempts the "true" artistic approach. The
{something} Matisse product, however, can get really slow with larger images.
I have found it useless for work but a gas to play with.

There are a number of public domain systems which do limited image
manipulation -- scaling, rotating, cropping, etc. Some of these have
their own little areas of surprise. I have already discussed the
pbmplus/netpbm libraries. There are others, such as ImageMagick, Utah
Raster Toolkit, etc. which provide similar routines.

For integration of images into presentations and some buffing for
publication (cropping, playing with backgrounds, etc.) I use
Corel, though many also like Harvard Graphics. I just happened to have
Corel at home, and never wanted to bother with the learning curve for
Harvard Graphics or PowerPoint. My wife uses Harvard Graphics and I have
helped her on occasion -- it is fairly intuitive.

For 3D modeling work, I have worked with AutoCAD, 3d Studio, and Lightwave
3D. Each has specific strengths and weaknesses, but I bet you aren't
interested in these, so I will spare you reviews. For high-end production
on UNIX boxes, there are Wavefront, Alias, SoftImage, and ElectroGig. I
have worked with Wavefront and Gig, if you want reviews.

Finally, there are specific tools for such things as texture generation,
3D painting, titling, etc. For PCs, for instance, Kai's Power Tools is
an excellent product. For UNIX boxes, Xaos has recently released a
number of good tools.

3D visualization tools for medical images which incorporate some
measurement capability include some commercial products which have been
discussed ad-nauseum on the confocal list, so I won't discuss them here.
There is a pseudo-commercial product out of Mayo called Analyze which is
primarily a 3D visualization tool with some measurement capability. Last I
heard, Rich Robb was charging about 5000 bucks for it. There is a
relatively cheap tool from UPenn (Jay Udupa's lab) called 3DViewnix which
runs about a thousand bucks which again is primarily a visualization tool
with some measurement capability. There is a free tool out of Arie
Kaufmann's lab at SUNY called VolVis which is, again, primarily a
visualization tool with some measurement capability. I have heard good
things about a program called Confocal Assistant, but have never seen or
tried it. I believe it is freely distributable, but I am not sure.


hope this helps,

billo


All opinions are my own and do not necessarily reflect those of the US
Govt, Army, Uncle Sam, yada, yada, yada.

On Thu, 11 Jan 1996 HAHNP-at-JEFLIN.TJU.EDU wrote:

} hello fellow digital microscopists.
} from snow bound Philly comes a query for advice on color image processing of
} images. does anyone use a package that can quantify/image process color data
} instead of just greyscale?
}
}
} please post replies directy and I will share summary with all.
}
}
}
} ._____________________
} | Peter J. Hahn
} | -------------
} | Thomas Jefferson University
} 3401 Spring Garden Street
} Philadelphia, PA. 19104
} tel : 215-349-0099 |
} fax : 610-356-3451 |
} peter.hahn-at-mail.tju.edu |
} -------------+-----------------+----+------+
}
}


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Message-Id: {n1390759410.38417-at-msmail.tmc.tulane.edu}

**** Very Important !!!!
My Telphone and Fax Numbers have been changed recently !!!

Zhen Quan Liu, Director, Ph.D Tel (86) 10 275 1427 (Office)
Physics Building Tel (86) 10 275 3727 (Home)
Electron Microscope Laboratory Fax (86) 10 275 1615 (Office)
Peking University
Beijing 100871, China E-mail(Office): zqliu-at-pku.edu.cn
Email (Home) wl-at-ibmstone.pku.edu.cn





From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Fri, 12 Jan 1996 09:24:20 -0200 (EDT)
Subject: Re: EM Fixative

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"Zaluzec, Nestor" {ZALUZEC-at-aaem.amc.anl.gov}
In-Reply-To: {n1390845623.57279-at-msmail.tmc.tulane.edu}
Message-ID: {Pine.3.87.9601120920.A26254-0100000-at-biomed}
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What exactly you want to know?
_____________________________________________________________________________
Francisco Javier Hernandez Blazquez * Av. Prof. Lineu Prestes, 1524
Departamento de Histologia e Embriologia * 05508-900 Sao Paulo
Instituto de Ciencias Biomedicas * e-mail fjhblazq-at-spider.usp.br
Universidade de Sao Paulo * fjhblasq-at-biomed.icb2.usp.br
______________________________________________________________________________






From: ghanem-solvay-at-e-mail.com
Date: Fri, 12 Jan 1996 06:36:40 EST
Subject: SEM : magnification

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Hello,

I am looking for references which contain detailed information about how the
magnification changes with working distance, spot size and voltage. On our
Cambridge Stereoscan II, I have noticed a "curious" behaviour, that is, for a
given set of microscope conditions, if we plot the real magnification as a
function of nominal magnification, we get maxima and minima which seem
reproducible. The range of magnification investigated is 100x to 10000x, no
sample tilt, secondary electrons signal, linear micrometer with 10 micron
spacings.

Thank you in advance for your help.

Antoine Ghanem
e-mail address : ghanem-solvay-at-e-mail.com

Extra X400 information begins:
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From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Fri, 12 Jan 1996 08:30:50 -500
Subject: 3x4 grey scale printers?

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Any suggestions?

I am looking for a small format (3-4" x 4-5" images, i.e. Polaroid
size) grey scale printer, that is NOT restricted to screen dumps or
Video dumps, but will function like a 'normal' printer output
device. It can be parallel port, serial port or SCSI.



Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu

int.




From: jlibert-at-cpcug.org (John M. Libert)
Date: Fri, 12 Jan 1996 07:17:04 -0500
Subject: Re: color image processing

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William R Oliver {oliver-at-ipas4.afip.mil}

At 10:40 PM 1/11/96 -0500, Kirk Rogers wrote:
} } NIH has a package called, I think, NIH Image, but I have never used it. I
} } have heard good things, but I think it is specific to Macs, is for
} } greyscale only, and is 8-bit only.
}
} I believe NIH Image supports 4, 8 and 16 bit TIFF (as well as PICT, pics,
} macpaint) images in both color and grayscale and 24 bit color images.
} Image will also open, edit, compare frames, average frames, etc. on
} captured video or quicktime movies. NIH Image now (v. 1.59) includes
} several built-in fast fourier transforms, although these run rather slowly
} on 68k Macintoshes. And heck, it's free!!
}
} {ftp://zippy.nimh.nih.gov/pub/nih-image/}
-----------------------------------------------------
Response:
** Actually, I believe that NIH Image currently cannot process color images
and cannot acquire color images except as single 8-bit channels. Possible
that someone has written some color extensions, but I am not aware of it.
Check before buying a new computer.
------------------------------------------------------------------------------
} The 8-bit part is in the way Image handles adjacent pixels. In other
} words, all contiguous pixels are counted during operations, not just the 4
} pixels (4-bit) that share a side with the pixel in question. In doing
} microstructural analysis of some acicular structures we found our $50k
} workstation-based image analysis system was not able to analyze the
} microstructure as well as NIH Image because it was a 4-bit system.
----------------------------------------------------------------}
Response:
**This is not correct. The 4, 8, 24 bit designation describes the number of
bits the program uses to represent the intensity value of each pixel, and
has nothing to do with how the pixel neighborhoods are processed. The number
of bits used to represent a numerical value in the computer is important to
how well the system can represent the image information and derived
information. The number of bits determine the range of values possible and
specifies the power of 2 topping the range. For example, with 4 bits one can
only represent 2^4 or 16 different values. With 8 bits the system can
represent 2^8 or 256 values. Generally, color images are represented as
three channels, each using 8-bits to quantize each pixel. Your old
workstation may have also had algorithm deficiencies in doing neighborhood
operations on images -- probably to save computation cost, but the main
problem may have been in allowing only 4 bits to represent the entire
dynamic range of your data.

** A second, but related, problem would arise if the system limits the bits
assigned to represent derived images. The best systems will use floating
point, or at least long integer, to represent intermediate values before the
derived image. If you wanted to average images or do some transformation,
you could run into serious problems if the values could be stored using 4 or
even 8 bits. Once truncated, the image information is lost.
------------------------------------------------------------------------------
} For those interested in doing 2D image analysis and reconstruction, The
} National Center for Supercomputing Applications (NCSA) at UIUC, has a
} program called NCSA Image. I just visited their web site,
}
} {http://www.ncsa.uiuc.edu/General/NCSAHome.html}
}
} and found that Image is now called Collage, and is available for Macintosh
} and X-Windows for FREE. Collage uses a proprietary data format, HDF, but
} you can get Import2HDF, which will convert FITS, TIFF, GIFF, Pict, ascii
} and rastor files to HDF. NCSA Collage also supports real time interaction
} between scientists working on the same data sets at remote locations
} according to the readme file.
}
} NCSA GelReader, which automates the measurement of DNA length using
} digitized electrophoretic gels is also available for free from NCSA, and is
} only available for the Macintosh.
}
} (Insert standard disclaimer about not being intimately involved with these
} products here.)
}
}
} -Kirk
} _____________________________________________
} Kirk Rogers krogers-at-materials.ecn.purdue.edu
} OR
} kirk.a.rogers.1-at-purdue.edu
} Purdue University, School of Materials Science and Engineering,
} 1289 MSEE building, W. Lafayette, IN 47907-1289
} OFFICE: 317-494-8751 FAX: 317-494-1204
} http://materials.ecn.purdue.edu/~krogers
}
}
}
}





From: dan-at-clark.isrv.com (Dan Focht)
Date: Fri, 12 Jan 1996 10:38:22 -0500
Subject: Subscribe

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From: Gisele Larocque :      LAROCQUEG-at-em.agr.ca
Date: Fri, 12 Jan 1996 09:58:33 -0500
Subject: Used Microscope

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** High Priority **

Hi,
We are looking to purchase a used scanning microscope such as
an AMR100 on which we can install a cold stage. We must take
delivery and pay for the microscope by the end of March 1996. If
anyone can tell us who could supply us with such a microscope
this quickly please reply to me directly. We are located in
Ottawa Canada. Thank you.
Gisele Larocque
larocqueg-at-em.agr.ca






From: Serita Frey :      serita-at-NREL.ColoState.EDU
Date: Fri, 12 Jan 1996 11:15:14 -0700
Subject: Used Microscope

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subscribe microscopy Serita Frey




From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 12 Jan 1996 10:06:35 -0500 (EST)
Subject: Re: Optical microscopes

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} I specifically need one
} that has a calibrated focus knob so that I can make thickness meaurements in
} the 1-50 micron range by focusing on the front and back surfaces of our
} (transparent) samples.

Dear Joyce,
There are also thickness gauges which can do the job, if the samples
are flat and the thickness uniform (some can also measure samples of non-
uniform thickness). These may or may not be more expensive than used micro-
scopes.
Yours,
Bill Tivol




From: GREG VAUGHN MARTIN :      gmartin-at-welchlink.welch.jhu.edu
Date: Fri, 12 Jan 1996 10:19:38 -0500 (EST)
Subject: Re: Image Analysis

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Greg --
I have been using a PC based image analysis package from Imaging
Research Inc.(no relation)to do morphometry and automated gold particle
counting on digitized EM negs for a couple of years. The use of digital
image analysis (IA) vs. point counting is highly dependant on your images
and what information you want to extract from them. If the structures of
interest can be easily segmented from the rest of the image, then IA is
the way to go. But if the measurement involves structures which need a
human eye and brain (still the best image analysis system avaliable) to
pick them out then point counting may be a much more efficient method.
An example of the latter would be measuring lengths of plasma
membrane sub-domains or the limiting membrane of non-descript
organelles. While you can trace the membranes using the cursor of an IA
system and get good data, its VERY tedious. You can do alot more
sampling with point counting techniques. If the specimen was immuno-gold
labeled, then IA would work well to count the gold particles along those
membranes since the computer can recognize the particles quite well
because they are very dense and have a very circular profile. But I
would still count them manually if there weren't too many.
There are IA systems out there that allow you to use point
counting methods on digital images. Imaging Reserch has an add-on to
their systems that does stereology, but I haven't had a chance to try it
out yet. I suspect that the ability to segment out the structures of
interest will still be crucial, at least for automated measurements. But as
long as you can manually define "hits" of the sampling grid on the
specimen these systems may be pretty useful.
Sorry for being long-winded, I'm alone in a snow-bound lab.

Greg Martin
Dept. Cell Biology and Anatomy
Johns Hopkins School of Medicine
gmartin-at-welchlink.welch.jhu.edu
On Tue, 9 Jan
1996 Gargent-at-aol.com wrote:

} Are there any EM people heavy into Image processing and analysis. Currently
} I am using a Kontron IBAS (version 2.0) and a KS400 Image analysis system.
} Soon we will be incorporating a Telepathology system for communications
} between the US, Switzerland, and Japan. Current Image imput for our routine
} analysis come from EM micrographs, stored images, Kodak digital camera, from
} the light microscope, and any source that allows us to capture, store, and
} process images. We have several video camera, including a Sony 960 color
} camera, B&W camera. We currently use PAL and NTSC formats.
}
} I would be interested in knowing how people use computerized Image Analysis
} for Stereology and Morphometry. Would be interested in knowing how to get
} around point counting techniques with Electron Micrographs.
}
} I would be willing to share IBAS macros with interested parties.
} Gregory Argentieri
} Sandoz Electron Microscopy lab
} East Hanover, NJ 07936
} 201-503-8617
} Greg2NJ-at-AOL.COM
}
}
}
} Would welcome interested parties to exchange macros, ideas, image processing
} tips etc.
}




From: GREG VAUGHN MARTIN :      gmartin-at-welchlink.welch.jhu.edu
Date: Fri, 12 Jan 1996 10:19:38 -0500 (EST)
Subject: Re: Image Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greg --
I have been using a PC based image analysis package from Imaging
Research Inc.(no relation)to do morphometry and automated gold particle
counting on digitized EM negs for a couple of years. The use of digital
image analysis (IA) vs. point counting is highly dependant on your images
and what information you want to extract from them. If the structures of
interest can be easily segmented from the rest of the image, then IA is
the way to go. But if the measurement involves structures which need a
human eye and brain (still the best image analysis system avaliable) to
pick them out then point counting may be a much more efficient method.
An example of the latter would be measuring lengths of plasma
membrane sub-domains or the limiting membrane of non-descript
organelles. While you can trace the membranes using the cursor of an IA
system and get good data, its VERY tedious. You can do alot more
sampling with point counting techniques. If the specimen was immuno-gold
labeled, then IA would work well to count the gold particles along those
membranes since the computer can recognize the particles quite well
because they are very dense and have a very circular profile. But I
would still count them manually if there weren't too many.
There are IA systems out there that allow you to use point
counting methods on digital images. Imaging Reserch has an add-on to
their systems that does stereology, but I haven't had a chance to try it
out yet. I suspect that the ability to segment out the structures of
interest will still be crucial, at least for automated measurements. But as
long as you can manually define "hits" of the sampling grid on the
specimen these systems may be pretty useful.
Sorry for being long-winded, I'm alone in a snow-bound lab.

Greg Martin
Dept. Cell Biology and Anatomy
Johns Hopkins School of Medicine
gmartin-at-welchlink.welch.jhu.edu
On Tue, 9 Jan
1996 Gargent-at-aol.com wrote:

} Are there any EM people heavy into Image processing and analysis. Currently
} I am using a Kontron IBAS (version 2.0) and a KS400 Image analysis system.
} Soon we will be incorporating a Telepathology system for communications
} between the US, Switzerland, and Japan. Current Image imput for our routine
} analysis come from EM micrographs, stored images, Kodak digital camera, from
} the light microscope, and any source that allows us to capture, store, and
} process images. We have several video camera, including a Sony 960 color
} camera, B&W camera. We currently use PAL and NTSC formats.
}
} I would be interested in knowing how people use computerized Image Analysis
} for Stereology and Morphometry. Would be interested in knowing how to get
} around point counting techniques with Electron Micrographs.
}
} I would be willing to share IBAS macros with interested parties.
} Gregory Argentieri
} Sandoz Electron Microscopy lab
} East Hanover, NJ 07936
} 201-503-8617
} Greg2NJ-at-AOL.COM
}
}
}
} Would welcome interested parties to exchange macros, ideas, image processing
} tips etc.
}




From: Warshall-at-aol.com
Date: Fri, 12 Jan 1996 18:00:10 -0500
Subject: SUBSCRIBE

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From: CASORIA-at-odin.ssec.honeywell.com
Date: Mon, 15 Jan 1996 10:50:52 -0600 (CST)
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casoria-at-ssec.honeywell.com




From: dan-at-clark.isrv.com (Dan Focht)
Date: Mon, 15 Jan 1996 00:55:58 -0500
Subject: Re: Subscribe

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I am interested in subscribing to this list. Will someone advise me as to
what I have to do to subscribe. Thanks in advance!

Daniel Focht
Bioptechs, Inc.
3560 Beck Road
Butler, PA 16001
dan-at-bioptechs.com
Web page for Live-Cell Microscopy products
http://www.bioptechs.com






From: kbart-at-itsmail1.hamilton.edu (Ken Bart)
Date: Mon, 15 Jan 1996 11:14:05 +0100
Subject: Re: Subscribe

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subscribe microscopy kbart-at-hamilton.edu

Kenneth M. Bart
Director, Electron Microscopy Facility
Hamilton College
Clinton, New York 13323 USA
kbart-at-hamilton.edu
(315) 859-4715






From: H.BRINKIES -SE108/TEL.8657 :      HANS-at-mechman.mm.swin.edu.au
Date: Mon, 15 Jan 1996 12:05:51 EST+10
Subject: cataracts

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Message-Id: {199601151420.IAA18934-at-Sparc5.Microscopy.Com}

I have tried various reflectors to receive some answers to the
following question but to date without any success. Here it is again:

Is there any evidence available that the extensive use of TEM's
and/or SEM's may lead to the development of cataracts ?

Thanking in advance for any reply.

Hans Brinkies




From: Brett W. Cockman :      bcockman-at-uniwa.uwa.edu.au
Date: Mon, 15 Jan 1996 12:13:04 +0700 (WAST)
Subject: Low viscosity nitrocellulose

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Gooday all!
I am seeking some help with locating a source of 'low viscosity
nitrocellulose (LVN)'. I have used it some years ago to embed large
specimens (approx. 10cm x 12cm x 10cm) for histology. LVN was obtainable
from BDH but apparently they stopped selling it in 1991. I wish to find an
alternative source of LVN or a suitable alternative embedding material.
Any help would be appreciated.
Thanks in advance.

Brett Cockman
Brett W. Cockman
Technologist in Charge
School of Dentistry
University of Western Australia
179 Wellington st., Perth, W.A. 6000
Voice: (619-2205834)
Fax: (619-2213829)
e-mail; bcockman-at-uniwa.uwa.edu.au




From: philf-at-NEWTON.UMSL.EDU (Phil Fraundorf)
Date: Mon, 15 Jan 1996 08:48:54 -0600
Subject: a few remarkable TEM facts

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Message-Id: {9601151447.AA29788-at-newton.umsl.edu.noname}
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Lonely electrons: I think it was John Armstrong of Caltech who once
pointed out to me that the number of microscope beam electrons in your TEM
specimen at any one time is so small that the odds of such electrons
interfering with each other to form diffraction patterns is quite small.
Under some illumination conditions there may be no more than 1 beam electron
in the column at a time! Hence diffraction patterns in the TEM are
basically formed by individual electrons interfering with themselves! As
you know, such interference will occur only if we don't take steps to
determine the path of individual electrons through the specimen! If we look
too closely at these paths, the diffraction patterns would disappear (cf. a
Sci. Amer. ariticle in the past year or so on quantum erasure).

Fat electrons: The transverse coherence widths of electrons which make
possible electron phase contrast (HREM) lattice imaging and probably
electron holography might also be seen as lateral broadening of individual
electron wave-packets via the uncertainty principle, which results because
we know too much about their transverse momentum! My intuition tells we
that we're talking about lateral wave-function spreads of say 15 Angstroms
in a LAB6 HREM to more than 100 Angstroms in field emission gun system. Are
these numbers reasonable? By increasing the spread of electron angles in
the incident beam, this transverse coherence width can presumably be
decreased (e.g. you want it small for Z-contrast imaging, I think), or
varied as in the variable coherence-width strategies of Murray Gibson at U.
of I.

Long electrons: The tight tolerences on high voltage stability and the
emitted spread in electron energies means that our uncertainty in the
longitudinal momentum of TEM electrons is quite small, and hence again by
the uncertainty principle that the wave-packet spread in the direction of
motion for TEM electrons can be quite large. Distances of say 1000
Angstroms come to mind! The associated tight distribution of incident
electron energies decreases chromatic and instability damping of fine
details in CTEM and HREM images, so that for most applications you may want
your electrons "as long as possible". An exception might be in
variable-coherence strategies (mentioned above), where shorter electrons
might provide sensitivity to shorter-range vertical correlations.

Fast electrons: a back of the envelope calculation for 300 keV electrons
gives gamma = (300+511)/511 = 1.587, so that they travel at w = c
Sqr[1-(1/gamma)^2] = 0.777 c or [lightyears per inertial year] of elapsed
time. However, if we consider traveler (i.e. electron or proper) time for
such a speeding electron, this would give that they travel u = gamma*w =
1.232 [lightyears per traveler year] of elapsed time! With this spatial
4-vector velocity well over c, we're dealing with relativity in action! I
wonder how many g's of acceleration they experience in the electron gun in
order to get up to speed? For more on this subject, you might want to check
our browser-interactive relativistic Accel-One problem solver at
{http://newton.umsl.edu/~run/index.html} , and the theory pages attached.

The foregoing thoughts on lonely, fat, long and fast electrons are not
really things I've had time to think much about, but they are interesting,
and hence I would enjoy other perspectives on them, as well as suggestions
for other "remarkable TEM facts" to add to the list!

Cheers. /philf :)


//\/\/\/\---}
// Phil Fraundorf Physics & Astronomy/CME 314+5165044 philf-at-newton.umsl.edu
\\ B503 U.Missouri-SL St.Louis MO 63121 USA http://newton.umsl.edu/~philf
\\/\/\/\/\/\/\/---}





From: zqliu-at-pccms.pku.edu.cn (zhenquan Liu)
Date: Fri, 12 Jan 1996 18:12:05 -0800 (PST)
Subject: Re: Image Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greg --
I have been using a PC based image analysis package from Imaging
Research Inc.(no relation)to do morphometry and automated gold particle
counting on digitized EM negs for a couple of years. The use of digital
image analysis (IA) vs. point counting is highly dependant on your images
and what information you want to extract from them. If the structures of
interest can be easily segmented from the rest of the image, then IA is
the way to go. But if the measurement involves structures which need a
human eye and brain (still the best image analysis system avaliable) to
pick them out then point counting may be a much more efficient method.
An example of the latter would be measuring lengths of plasma
membrane sub-domains or the limiting membrane of non-descript
organelles. While you can trace the membranes using the cursor of an IA
system and get good data, its VERY tedious. You can do alot more
sampling with point counting techniques. If the specimen was immuno-gold
labeled, then IA would work well to count the gold particles along those
membranes since the computer can recognize the particles quite well
because they are very dense and have a very circular profile. But I
would still count them manually if there weren't too many.
There are IA systems out there that allow you to use point
counting methods on digital images. Imaging Reserch has an add-on to
their systems that does stereology, but I haven't had a chance to try it
out yet. I suspect that the ability to segment out the structures of
interest will still be crucial, at least for automated measurements. But as
long as you can manually define "hits" of the sampling grid on the
specimen these systems may be pretty useful.
Sorry for being long-winded, I'm alone in a snow-bound lab.

Greg Martin
Dept. Cell Biology and Anatomy
Johns Hopkins School of Medicine
gmartin-at-welchlink.welch.jhu.edu
On Tue, 9 Jan
1996 Gargent-at-aol.com wrote:

} Are there any EM people heavy into Image processing and analysis. Currently
} I am using a Kontron IBAS (version 2.0) and a KS400 Image analysis system.
} Soon we will be incorporating a Telepathology system for communications
} between the US, Switzerland, and Japan. Current Image imput for our routine
} analysis come from EM micrographs, stored images, Kodak digital camera, from
} the light microscope, and any source that allows us to capture, store, and
} process images. We have several video camera, including a Sony 960 color
} camera, B&W camera. We currently use PAL and NTSC formats.
}
} I would be interested in knowing how people use computerized Image Analysis
} for Stereology and Morphometry. Would be interested in knowing how to get
} around point counting techniques with Electron Micrographs.
}
} I would be willing to share IBAS macros with interested parties.
} Gregory Argentieri
} Sandoz Electron Microscopy lab
} East Hanover, NJ 07936
} 201-503-8617
} Greg2NJ-at-AOL.COM
}
}
}
} Would welcome interested parties to exchange macros, ideas, image processing
} tips etc.
}


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**** Very Important !!!!
My Telphone and Fax Numbers have been changed recently !!!

Zhen Quan Liu, Director, Ph.D Tel (86) 10 275 1427 (Office)
Physics Building Tel (86) 10 275 3727 (Home)
Electron Microscope Laboratory Fax (86) 10 275 1615 (Office)
Peking University
Beijing 100871, China E-mail(Office): zqliu-at-pku.edu.cn
Email (Home) wl-at-ibmstone.pku.edu.cn





From: jbpawley-at-facstaff.wisc.edu (Jim Pawley)
Date: Mon, 15 Jan 1996 12:20:52 -0600
Subject: Re: SEM magnification

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Antoine,

If you haven't received many replies to your magnification problem, it may
be because there are many sources of possible error in SEM magnification.
What the mag circuit does is define the current through the scan coils and
these establish a maximum scan ANGLE that raster will have as it leaves the
final lens. How big an AREA this raster will cover on the specimen depends
on how far the specimen is below the lens (the working distance, WD) and
the square-root of the energy of the electrons in the beam.

Old SEMs where designed to only work at one working distance to eliminate
the WD variable. Later it was found that one could get a measure of the WD
(at a given beam energy and assuming no hysteresis in the magnetic circuit
of the objective lens) by measuring the current in the final lens and later
instruments used this information internally to compensate the scan
current. Likewise the scan-currents were normalized to provide the same
scan angles first at a few, and then later at a large variety of beam
voltages.

To make all of this compensation work, there are a number of potentiometers
in the scanning amplifier to allow the magnification to be adjusted in x
and y under a number of standard conditions (certain Mags, WDs and kVs).
It would seem at first glance that these pots may not be prperly adjusted
on your instrument.

Normally SEMs are adjusted so that the magnification refers to the ratio of
the length of a feature on a photograph (usually a Polaroid) produced by
the microscopy divided by the length of the same feature in real life on
the specimen. You have added the additional complication of a video
printer. These can be useful but often only operate at video-scan-rate
where SEM image distortion is high and where the printer makes specific
assumptions aboutthe dimensions of the TV raster (number of lines, ration
of H to V) that the SEM Manufacturer does not fulfill (and this is
particularly true if you use a mixture of PAL (625 line) and NTSC (525
lines) equipment). You may also be using a computer-based image capture
system that has a video-rate display which you print with your printer. On
the assumption that this image-capture system is active (generates the
scanning signals) rather than passive (listens to the scan signals
generated by the microscope electronics) it seems likely that the
misadjustments are in the scan-generation software of your image capture
system rather than in the potentiometers noted above.

A lot of this information can be found a good basic SEM text.

Good LUck,

Jim Pawley

****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU






From: ghanem-solvay-at-e-mail.com
Date: Mon, 15 Jan 1996 03:33:36 EST
Subject: SEM magnigication

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Hello,

I realized that my first message about maxima and minima in SEM magnification
might have been misleading, so I would like to provide some details. What we
plot is the ratio of measured and nominal magnifications as a function of
nominal magnification. The measured values come from black and white
videoprints (Sony UP-850). The ratio ranges betwwen 0.75 and 0.94, depending on
the microscope setting. The average and standard deviation of 73 ratios are
0.83 and 0.05, respectively. The curves exhibit the following behaviour :
increase to a maximum about 200x, followed by a decrease to a mimium about
1000x, then an increase to about 3000x followed by a decrease down to 10000x.
Please note that the resolution of the curve is rather low (8 data points in
total), and it's not always easy to measure the magnification below 200x with a
10 micron spacing micrometer (which means the maximum at 200x is only observed
when there are data points below 200x).

I hope this rings a bell to somebody...

Regards,

Antoine Ghanem
ghanem-solvay-at-e-mail.com

Extra X400 information begins:
Originator
Name: Antoine (AGM) GHANEM
Org Units: AC
: NOH
: LC-AN001
Organisation: NOHX400DEC
Domain: BE/RTT/SOLVAY
Node.Userid: IBMX400.184470

Message Id: 65239051106991/91343 MHS
Importance: Normal
Sent by
Name: Antoine (AGM) GHANEM
Org Units: AC
: NOH
: LC-AN001
Organisation: NOHX400DEC
Domain: BE/RTT/SOLVAY
Node.Userid: IBMX400.184470
Free Fmt Name: Antoine GHANEM
Phone Number: 3422
Subject: SEM magnigication
Recipients
Name: INTERNET INTERNET
Domain: GB/IBMX400/IBMMAIL
Node.Userid: IBMMAIL.INTERNET
Free Fmt Name: INTERNET INTERNET




From: ghanem-solvay-at-e-mail.com
Date: Mon, 15 Jan 1996 08:10:31 EST
Subject: SEM magnification

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I realized that my first message about maxima and minima in SEM magnification
might have been misleading, so I would like to provide some details. What we
plot is the ratio of measured and nominal magnifications as a function of
nominal magnification. The measured values come from black and white
videoprints (Sony UP-850). The ratio ranges betwwen 0.75 and 0.94, depending on
the microscope setting. The average and standard deviation of 73 ratios are
0.83 and 0.05, respectively. The curves exhibit the following behaviour :
increase to a maximum about 200x, followed by a decrease to a mimium about
1000x, then an increase to about 3000x followed by a decrease down to 10000x.
Please note that the resolution of the curve is rather low (8 data points in
total), and it's not always easy to measure the magnification below 200x with a
10 micron spacing micrometer (which means the maximum at 200x is only observed
when there are data points below 200x).

I hope this rings a bell to somebody...

Regards,

Antoine Ghanem
ghanem-solvay-at-e-mail.com

Extra X400 information begins:
Originator
Name: Antoine (AGM) GHANEM
Org Units: AC
: NOH
: LC-AN001
Organisation: NOHX400DEC
Domain: BE/RTT/SOLVAY
Node.Userid: IBMX400.184470

Message Id: 50704151106991/92175 MHS
Importance: Normal
Sent by
Name: Antoine (AGM) GHANEM
Org Units: AC
: NOH
: LC-AN001
Organisation: NOHX400DEC
Domain: BE/RTT/SOLVAY
Node.Userid: IBMX400.184470
Free Fmt Name: Antoine GHANEM
Phone Number: 3422
Subject: SEM magnification
Recipients
Name: INTERNET INTERNET
Domain: GB/IBMX400/IBMMAIL
Node.Userid: IBMMAIL.INTERNET
Free Fmt Name: INTERNET INTERNET




From: jaakko-at-butler.cc.tut.fi (Jaakko Ker\dnen)
Date: Mon, 15 Jan 1996 09:06:37 +0200
Subject: subscribe

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Microscopy ListServ {microscopy-at-Sparc5.Microscopy.Com}
Message-id: {01I01501O90I95RFNU-at-MAIL-CLUSTER.PCY.MCI.NET}
X-Mailer: e-mailMCI v2.3
Content-transfer-encoding: 7BIT

-- [ From: Scott Ireland * EMC.Ver #2.3 ] --

Nancy - There is an error in the Web address listed - it should be
ddsdx.uthscsa.edu, not ddxds.uthscsa.edu. It took me almost an hour to
find it, then I realized what was wrong. Hope everyone else finds it ok -

BTW - I have tried to subsribe to the ,mail list for IT as listed on the
Web page, but I keep getting error messages back - Any ideas?

Scott Ireland
Oncor Imaging

-------- REPLY, Original message follows --------


sumscribe jaakko-at-butler.cc.tut.fi





From: houpt-at-worldaccess.nl (houpt)
Date: Sat, 13 Jan 1996 13:41:09 +0100
Subject: microscopy list

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subscribe microscopy






From: zqliu-at-pccms.pku.edu.cn (zhenquan Liu)
Date: Fri, 12 Jan 1996 18:12:05 -0800 (PST)
Subject: Re: Image Analysis

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Greg --
I have been using a PC based image analysis package from Imaging
Research Inc.(no relation)to do morphometry and automated gold particle
counting on digitized EM negs for a couple of years. The use of digital
image analysis (IA) vs. point counting is highly dependant on your images
and what information you want to extract from them. If the structures of
interest can be easily segmented from the rest of the image, then IA is
the way to go. But if the measurement involves structures which need a
human eye and brain (still the best image analysis system avaliable) to
pick them out then point counting may be a much more efficient method.
An example of the latter would be measuring lengths of plasma
membrane sub-domains or the limiting membrane of non-descript
organelles. While you can trace the membranes using the cursor of an IA
system and get good data, its VERY tedious. You can do alot more
sampling with point counting techniques. If the specimen was immuno-gold
labeled, then IA would work well to count the gold particles along those
membranes since the computer can recognize the particles quite well
because they are very dense and have a very circular profile. But I
would still count them manually if there weren't too many.
There are IA systems out there that allow you to use point
counting methods on digital images. Imaging Reserch has an add-on to
their systems that does stereology, but I haven't had a chance to try it
out yet. I suspect that the ability to segment out the structures of
interest will still be crucial, at least for automated measurements. But as
long as you can manually define "hits" of the sampling grid on the
specimen these systems may be pretty useful.
Sorry for being long-winded, I'm alone in a snow-bound lab.

Greg Martin
Dept. Cell Biology and Anatomy
Johns Hopkins School of Medicine
gmartin-at-welchlink.welch.jhu.edu
On Tue, 9 Jan
1996 Gargent-at-aol.com wrote:

} Are there any EM people heavy into Image processing and analysis. Currently
} I am using a Kontron IBAS (version 2.0) and a KS400 Image analysis system.
} Soon we will be incorporating a Telepathology system for communications
} between the US, Switzerland, and Japan. Current Image imput for our routine
} analysis come from EM micrographs, stored images, Kodak digital camera, from
} the light microscope, and any source that allows us to capture, store, and
} process images. We have several video camera, including a Sony 960 color
} camera, B&W camera. We currently use PAL and NTSC formats.
}
} I would be interested in knowing how people use computerized Image Analysis
} for Stereology and Morphometry. Would be interested in knowing how to get
} around point counting techniques with Electron Micrographs.
}
} I would be willing to share IBAS macros with interested parties.
} Gregory Argentieri
} Sandoz Electron Microscopy lab
} East Hanover, NJ 07936
} 201-503-8617
} Greg2NJ-at-AOL.COM
}
}
}
} Would welcome interested parties to exchange macros, ideas, image processing
} tips etc.
}


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**** Very Important !!!!
My Telphone and Fax Numbers have been changed recently !!!

Zhen Quan Liu, Director, Ph.D Tel (86) 10 275 1427 (Office)
Physics Building Tel (86) 10 275 3727 (Home)
Electron Microscope Laboratory Fax (86) 10 275 1615 (Office)
Peking University
Beijing 100871, China E-mail(Office): zqliu-at-pku.edu.cn
Email (Home) wl-at-ibmstone.pku.edu.cn





From: Larry Maser :      lmaser-at-mbl.edu
Date: Sun, 14 Jan 1996 00:08:56 -0500 (EST)
Subject: Microscopy & Microanalysis '96, Minneapolis

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Microscopy & Microanalysis '96, the joint 54th Annual Meeting of
the Microscopy Society of America, 30th of the Microbeam Analysis
Society and the 23rd of the Microscopical Society of Canada /
Societe de Microscopie du Canada, will be held August 11-15,
1996, in Minneapolis, Minnesota.

The deadline for receipt of papers (extended abstracts) is March
15, 1996. The Registration Bulletin / Call for Papers will be
mailed automatically to members of the three Societies by the end
of January.

For information contact:

Microscopy & Microanalysis '96
4 Barlows Landing Rd., Suite 8
Pocasset, MA 02644

toll free 800-538-3672
phone 508-563-1155
fax 508-563-1211
email BusinessOffice-at-MSA.Microscopy.Com




From: krogers-at-ecn.purdue.edu (Kirk Rogers)
Date: Fri, 12 Jan 1996 15:18:03 -0500
Subject: Re: Import/processing questions

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John and List Members-

I posed the following questions to the nih-image mailing list to clear up
some of the current discussion. More info on the mailing list can be found
at the bottom of the page. The response is from wayne Rasband, the author
of NIH Image.

} Date: Fri, 12 Jan 1996 13:03:30 -0600 (CST)
} Reply-To: nih-image-at-Soils.Umn.EDU
} Originator: nih-image-at-soils.umn.edu
} Sender: nih-image-at-Soils.Umn.EDU
} Precedence: bulk
} From: wayne-at-helix.nih.gov (Wayne Rasband)
} To: Multiple recipients of list {nih-image-at-Soils.Umn.EDU}
} Subject: Re: Import/processing questions
} X-Comment: NIH Image Distribution List
} Mime-Version: 1.0
} Status: O
}
} } Hey-
} }
} } On the microscopy list server (Microscopy-at-MSA.Microscopy.Com) we are
} } currently having a discussion about Image's capabilities. They are as
} } follows.
} }
} } 1. NIH Image supports 4, 8 and 16 bit TIFF (as well as PICT, pics,
} } macpaint) images in both color and grayscale and 24 bit color images. Is
} } that right?
}
} NIH Image can read 4, 8 and 16 bit grayscale TIFF files as well as 24 bit
} color TIFF files. It can't read compressed TIFF fles.
}
} } has any one written macros for importing 30 bit (or more) color
} } (or Greyscale) TIFF images?
}
} Not that I know of. Do such TIFF files exist?
}
} } I looked in the macros file on zippy, but none
} } of them seem to do just this?
}
} } 2. Image processes 8 pixel neighborhoods surrounding the central pixel.
}
} The built-in filters process 3x3 (8 pixel) neighborhoods. The Convolve
} command supports user defined kernels up to 63x63 (3968 pixel
} neighborhoods).
}
} }
} } 3. Image uses either 8 or 16 bit integers, depending on the input image,
} } when doing image math, yes or no?
}
} The Image Math command uses real operations but scales the result to
} 8-bits. V1.59 adds the ability to process 32-bit real images.
}
} } 4. UTHSCSA ImageTool for Windows sounds very much like Image, does it use
} } NIH Image source code?
}
} I doubt it uses any nih-image source code since it's written in C++
} (nih-image is written in Pascal) and it doesn't look much like nih-image.
} It does, however, appear to be in the spirit of nih-image. There is a link
} to the Image Tool home page on the nih-image home page
} (http://rsb.info.nih.gov/nih-image/).
}
} --wayne

Join the NIH-Image mailing list. by sending a message to
listproc-at-soils.umn.edu with subscribe nih-image {first name} {last name} in
the message body.

archived email (since 09/93) is available at the following:

Searchable index:
{gopher://gopher.soils.umn.edu:7151/77/wais-sources/nih-image-archive}

Browse messages by date:
{ftp://ftp.soils.umn.edu:pub/info/email-lists/nih-image/}
{gopher://gopher.soils.umn.edu:10082/11/email-lists/nih-image}


-Kirk
_____________________________________________
Kirk Rogers krogers-at-materials.ecn.purdue.edu
OR
kirk.a.rogers.1-at-purdue.edu
Purdue University, School of Materials Science and Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289
OFFICE: 317-494-8751 FAX: 317-494-1204
http://materials.ecn.purdue.edu/~krogers






From: Nancy K. R. Smith :      SMITHN-at-UTHSCSA.EDU
Date: Fri, 12 Jan 1996 15:46:02 -0500 (CDT)
Subject: IMAGETOOL

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Hopefully the attached message from Brent Dove will clarify instructions for
downloading Image Tool (IT), free image analysis software for Windows 95 and
Windows NT....nkrs

} Return-path: {DOVE-at-uthscsa.edu}
} Date: Fri, 12 Jan 1996 11:03:30 -0500 (CDT)
} Date-warning: Date header was inserted by uthscsa.edu
} From: "S. Brent Dove" {dove-at-uthscsa.edu}
} Subject: IMAGETOOL
} To: Nancy Smith {smithn-at-uthscsa.edu}
}
} Hello Nancy,
}
} I am being inundated with email. Please update your listserver with the
following information. About how to get UTHSCSA ImageTool IT is available
via anonymous ftp at=20
}
} ftp://maxrad6.uthscsa.edu
}
} Please do not send email to dove-at-uthscsa.edu
}
} I have enclosed the original write up including the ftp address at the
bottom. Sorry that was my fault for not including the ftp address. Thank
you for getting the information to other microscopist.
}
} UTHSCSA
} IMAGETOOL
}
} OVERVIEW
}
} UTHSCSA ImageTool (IT) is a free image processing and analysis program for
Microsoft Windows 95=81 or Windows NT=81. IT can acquire, display, edit,
analyze, process, compress, save and print gray scale and color images. IT
can read and write over 22 common file formats including BMP, PCX, TIF, GIF
and JPEG. Image analysis functions include dimensional (distance, angle,
perimeter, area) and gray scale measurements (point, line and area histogram
with statistics). ImageTool supports standard image processing functions
such as contrast manipulation, sharpening, smoothing, edge detection, median
filtering and spatial convolutions with user-defined convolution masks. IT
also has built-in macro capabilities that allow the user to record
repetitive tasks and playback saved macros to automate image analysis.
}
} ImageTool was designed with an open architecture that provides extensiblity
via a variety of plug-ins. Support for image acquisition using either Adobe
Photoshop plug-ins or Twain scanners is built-in. Custom analysis and
processing plug-ins can be developed using the software development kit
(SDK) provided (with source code). This approach makes it possible to solve
almost any data acquisition or analysis problem with IT.
}
} ImageTool provides for geometric transformations such as rotate, flip
vertical, flip horizontal and magnification up to four levels. All analysis
and processing functions are available at any magnification factor. The
program is a multiple document interface (MDI) application supporting any
number of windows (images) simultaneously. =20
}
} Spatial calibration is available to indicate real world dimensional
measurements such as millimeters, microns, feet, miles, etc. for linear and
area. Density or gray scale calibration can be done relative to radiation
or optical density (OD) standards.
}
} IT version 1.1 now provides for object analysis and classification with
over 20 morphological descriptors such as: area/perimeter, roundness, ferret
diameter, compactness, major/minor axis length, centroid and many others.
Any of these factors can be used automatically categorized and count objects
within the image.
}
} ImageTool ver 1.1 supports the Data Translation DT3155 frame grabber for
Windows NT. Other frame grabber board will be added in the coming months.=
=20
}
} ImageTool was written using Borland's C++ version 4.5 and the source code
for the executable is available free of charge. IT was developed in the
Department of Dental Diagnostic Science at The University of Texas Health
Science Center, San Antonio, Texas. The program was developed by C. Donald
Wilcox, S. Brent Dove, W. Doss McDavid and David B. Greer.
}
} UTHSCSA ImageTool is availabel via anonymous ftp at=
ftp://maxrad6.uthscsa.edu
}
} S. Brent Dove Voice: (210) 567-3333
} Diagnostic Sciences Fax: (210) 567-3334
} University of Texas Email: dove-at-uthscsa.edu
} Health Science Center Web: ddxds.uthscsa.edu
} San Antonio, TX USA ftp: maxrad6.uthscsa.edu
}
}





From: Eric A. Rosen :      earosen-at-indirect.com
Date: Mon, 15 Jan 1996 20:25:48 +0000
Subject: Re: Things not to do while defending your Thesis (Humor)

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Message-Id: {199601160327.UAA00999-at-ns1.indirect.com}
Comments: Authenticated sender is {earosen-at-indirect.com}



Hi! Our graduate assistant had this floating around the school, not sure where
he found it. I thought you might appreciate this considering you're working
on your thesis.

150 THINGS (NOT) TO DO OR SAY AT OR FOR YOUR THESIS DEFENSE
}
1) "Ladies and Gentlemen, please rise for the singing of our National
Anthem..."
2) Charge 25 cents a cup for coffee.
3) "Charge the mound" when a professor beans you with a high fast question.
4) Describe parts of your thesis using interpretive dance.
5) "Musical accompaniment provided by..."
6) Stage your own death/suicide.
7) Lead the specators in a Wave.
8) Have a sing-a-long.
9) "You call THAT a question? How the hell did they make you a professor?"
10) "Ladies and Gentlemen, as I dim the lights, please hold hands and
concentrate so that we may channel the spirit of Lord Kelvin..."
11) Have bodyguards outside the room to "discourage" certain professors
from sitting in.
12) Puppet show.
13) Group prayer.
14) Animal sacrifice to the god of the Underworld.
15) Sell T-shirts to recoup the cost of copying, binding, etc.
16) "I'm sorry, I can't hear you - there's a banana in my ear!"
17) Imitate Groucho Marx.
18) Mime.
19) Hold a Tupperware party.
20) Have a bikini-clad model be in charge of changing the overheads.
21) "Everybody rhumba!!"
22) "And it would have worked if it weren't for those meddling kids..."
23) Charge a cover and check for ID.
24) "In protest of our government's systematic and brutal opression of
minorities..."
25) "Anybody else as drunk as I am?"
26) Smoke machines, dramatic lighting, pyrotechnics...
27) Use a Super Soaker to point at people.
28) Surreptitioulsy fill the room with laughing gas.
29) Door prizes and a raffle.
30) "Please phrase your question in the form of an answer..."
31) "And now, a word from our sponsor..."
32) Present your entire talk in iambic pentameter.
33) Whine piteously, beg, cry...
34) Switch halfway through your talk to Pig Latin. Or Finnish Pig Latin.
35) The Emperor's New Slides ("only fools can't see the writing...")
36) Table dance (you or an exotic dancer).
37) Fashion show.
38) "Yo, a smooth shout out to my homies..."
39) "I'd like to thank the Academy..."
40) Minstrel show (blackface, etc.).
41) Previews, cartoons, and the Jimmy Fund.
42) Pass the collection basket.
43) Two-drink minimum.
44) Black tie only.
45) "Which reminds me of a story - A Black guy, a Chinese guy, and a
Jew walked into a bar..."
46) Incite a revolt.
47) Hire the Goodyear Blimp to circle the building.
48) Release a flock of doves.
49) Defense by proxy.
50) "And now a reading from the Book of Mormon..."
51) Leave Jehovah's Witness pamphlets scattered about.
52) "There will be a short quiz after my presentation..."
53) "Professor Walcerz, will you marry me?"
54) Bring your pet boa.
55) Tell ghost stories.
56) Do a "show and tell".
57) Food fight.
58) Challenge a professor to a duel. Slapping him with a glove is optional.
59) Halftime show.
60) "Duck, duck, duck, duck... GOOSE!"
61) "OK - which one of you farted?"
62) Rimshot.
63) Sell those big foam "We're number #1" (sic) hands.
64) Pass out souvenir matchbooks.
65) 3-ring defense.
66) "Tag - you're it!"
67) Circulate a vicious rumor that the Dead will be opening, making sure that
it gets on the radio stations, and escape during all the commotion.
68) Post signs: "Due to a computer error at the Registrar's Office, the
original room is not available, and the defense has been relocated to
(Made-up non-existent room number)"
69) Hang a pinata over the table and have a strolling mariachi band.
70) Make each professor remove an item of clothing for each question he asks.
71) Rent a billboard on the highway proclaiming "Thanks for passing me
Professors X,Y, and Z" - BEFORE your defense happens.
72) Have a make-your-own-sundae table during the defense.
73) Make committee members wear silly hats.
74) Simulate your experiment with a virtual reality system for the
spectators.
75) Do a soft-shoe routine.
76) Throw a masquerade defense, complete with bobbing for apples and
pin-the-tail-on-the-donkey.
77) Use a Greek Chorus to highlight important points.
78) "The responsorial psalm can be found on page 124 of the thesis..."
79) Tap dance.
80) Vaudeville.
81) "I'm sorry Professor Smith, I didn't say 'SIMON SAYS any questions?'.
You're out."
82) Flex and show off those massive pecs.
83) Dress in top hat and tails.
84) Hold a pre-defense pep rally, complete with cheerleaders, pep band, and
a bonfire.
85) Detonate a small nuclear device in the room. Or threaten to.
86) Shadow puppets.
87) Show slides of your last vacation.
88) Put your overheads on a film strip. Designate a professor to be in
charge of turning the strip when the tape recording beeps.
89) Same as #88, but instead of a tape recorder, go around the room
making a different person read the pre-written text for each picture.
90) "OK, everybody - heads down on the desk until you show me you can behave."
91) Call your advisor "sweetie".
92) Have everyone pose for a group photo.
93) Instant replay.
94) Laugh maniacally.
95) Talk with your mouth full.
96) Start speaking in tongues.
97) Explode.
98) Implode.
99) Spontaneously combust.
100) Answer every question with a question.
101) Moon everyone in the room after you are done.
102) "Laugh, will you? Well, they laughed at Galileo, they laughed at
Einstein..."
103) Hand out 3-D glasses.
104) "I'm rubber, you're glue..."
105) Go into labor (especially for men).
106) Give your entire speech in a "Marvin Martian" accent.
107) "I don't know - I didn't write this."
108) Before your defense, build trapdoors underneath all the seats.
109) Swing in through the window, yelling a la Tarzan.
110) Lock the department head and his secretary out of the defense room. And
the coffee lounge, the department office, the copy room, and the mail
room. Heck, lock them out of the building. And refuse to sell them
stamps (NOTE: This is an inside gripe, based on conditions that
existed in the ME department at WPI while we were there. Sorry.)
111) Roll credits at the end. Include a "key grip", and a "best boy".
112) Hang a disco ball in the center of the room. John Travolta pose optional.
113) Invite the homeless.
114) "I could answer that, but then I'd have to kill you"
115) Hide.
116) Get a friend to ask the first question. Draw a blank-loaded gun and
"shoot" him. Have him make a great scene of dying (fake blood helps).
Turn to the stunned audience and ask "any other wise-ass remarks?"
117) Same as #116, except use real bullets.
118) "Well, I saw it on the Internet, so I figured it might be a good idea..."
119) Wear clown makeup, a clown wig, clown shoes, and a clown nose. And
nothing else.
120) Use the words "marginalized", "empowerment", and "patriarchy".
121) Play Thesis Mad Libs.
122) Try to use normal printed paper on the overhead projector.
123) Do your entire defense operatically.
124) Invite your parents. Especially if they are fond of fawning over you.
("We always knew he was such an intelligent child")
125) Flash "APPLAUSE" and "LAUGHTER" signs.
126) Mosh pit.
127) Have cheerleaders. ("Gimme an 'A'!!")
128) Bring Howard Cosell out of retirement to do color commentary.
(NOTE: Because of recent events, this has to be changed to "Bring
Howard Cosell back from the dead to do color commentary.")
129) "I say Hallelujah, brothers and sisters!"
130) Claim political asylum.
131) Traffic reports every 10 minutes on the 1's.
132) Introduce the "Eyewitness Thesis Team". Near the end of your talk, cut
to Jim with sports and Alison with the weather.
133) Live radio and TV coverage.
134) Hang a sign that says "Thank you for not asking questions"
135) Bring a microphone. Point it at the questioner, talk-show style.
136) Use a TelePrompTer
137) "Take my wife - please!"
138) Refuse to answer questions unless they phrase the question as a limerick.
139) Have everyone bring wine glasses. When they clink the glasses with a
spoon, you have to kiss your thesis. Or your advisor.
140) Offer a toast.
141) Firewalk.
142) Start giving your presentation 15 minutes early.
143) Play drinking thesis games. Drink for each overhead. Drink for each
question. Chug for each awkward pause. This goes for the audience
as well.
144) Swoop in with a cape and tights, Superman style.
145) "By the power of Greyskull..."
146) Use any past or present Saturday Night Live catchphrase. Not.
147) Stand on the table.
148) Sell commercial time for your talk and ad space on your overheads.
149) Hold a raffle.
150) "You think this defense was bad? Let me read this list to show you
what I COULD have done..."
}
(FINAL NOTE: Depending on the subject of your thesis, some of these
things, such as tap dance, virtual reality, or reading from the Book
of Mormon might be entirely appropriate, of course.)
}

Cheers ;o) :o) %o)
Eric





From: Lijie Zhao :      lijie-at-junction.ucsb.edu
Date: Mon, 15 Jan 1996 20:57:03 -0800
Subject: Re: Things not to do while defending your Thesis (Humor)

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subscribe lijie-at-junction.ucsb.edu




From: Lijie Zhao :      lijie-at-junction.ucsb.edu
Date: Mon, 15 Jan 1996 22:43:06 -0800
Subject: Re: Things not to do while defending your Thesis (Humor)

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subscribe Lijie Zhao




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Tue, 16 Jan 1996 09:07:20 -0500
Subject: Re: cataracts

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To: microscopy-at-aaem.amc.anl.gov

} On 15 Jan 1996 Hans Brinkies wrote:
}
} } Is there any evidence available that the extensive use of TEM's
} } and/or SEM's may lead to the development of cataracts ?
} }
} }
} } Hans Brinkies
}
} This is an interesting point. Some time ago someone asked a similar
} question but I cannot remember seeing any discussion on the issue. Does
} anyone know about the long term health effects of EM usage? Have any studies
} been done on any aspect of this (eyesight, radiation exposure, anything else
} I haven't thought of)?
}
} Ian MacLaren,


EMSA (now MSA) published a "Handbook of X-ray safety for Electron
Microscopists" by D.F} Parsons, V.A. Phillips, and J.S. Lally in 1973. Its
a short pamphlet and I don't know if it is still available. A few points
from the handbook:

In regards to the need to perform an annual survey for x-ray leaks: "It
should be noted that about 50% of those monitoring their microscopes found
detectable leaks once or more". They reccomend film badges but I have
never been at an institution that did this for their EM people.

Under the Health Checks chapter: "An annual eye exam is desirable. Vision
defects should be recorded and the lenses checked for opacities
(cataracts)". No references or data supporting this statement are given.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 16 Jan 1996 09:58:31 -0500 (EST)
Subject: Re: a few remarkable TEM facts

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Dear Phil,
}
} Lonely electrons:
[snip]
} Under some illumination conditions there may be no more than 1 beam electron
} in the column at a time!

With our HVEM (1.2 MV) and its ~3m column this turns out to be cor-
rect--especially since we use low-dose conditions for ED work.

} Hence diffraction patterns in the TEM are
} basically formed by individual electrons interfering with themselves!

But wait; there's more! The electrons are boiled off our W filament
as particles, interfere with themselves as waves when scattering off the
crystal, then strike a film grain as particles. ED is the quintessential
demonstration of the particle-wave duality.

} Fat electrons: The transverse coherence widths of electrons which make
} possible electron phase contrast (HREM) lattice imaging and probably
} electron holography might also be seen as lateral broadening of individual
} electron wave-packets via the uncertainty principle, which results because
} we know too much about their transverse momentum! [snip]

What are the predictions for hollow-cone illumination where we know
the magnitude of the transverse momentum, but not its direction?

} [snip]
} Fast electrons: a back of the envelope calculation for 300 keV electrons
} gives gamma = (300+511)/511 = 1.587, so that they travel at w = c
} Sqr[1-(1/gamma)^2] = 0.777 c or [lightyears per inertial year] of elapsed
} time.

Of course, for 1.2 MeV electrons the speed is even closer to c.
Yours,
Bill Tivol




From: akracher-at-iastate.edu (Alfred Kracher)
Date: Tue, 16 Jan 1996 10:41:42 -0600
Subject: Image file conversion

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X-Sender: akracher-at-pop-1.iastate.edu
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Sorry for bugging once again all those helpful people who have given advice
on the conversion of image file formats. I specifically have a question
about AUTOCAD drawings. If anyone knows how to convert these to more common
image files, please get in touch with me directly.
Thanks, Alfred

Alfred Kracher
akracher-at-iastate.edu
http://www.public.iastate.edu/~akracher






From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Tue, 16 Jan 1996 11:43:56 -0500 (EST)
Subject: Re: dye sub gray scale

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Yes, the ribbons have changed. If you complain enough and to the right
person at Kodak, Kodak will send you a disk with new color look uop
tables that can be downloaded to the printer. Your b/w prints will come
out with a slight blue tint instead of the green tint, but the blue tint
is less objectionable.
-Michael Cammer
{null signature file}

On Thu, 11 Jan 1996, Jon Krupp wrote:

} Hi:
}
} Has anyone run into a problem printing grayscale images on a Kodak/Codonics
} printer?
}
} Ours have started looking tinged with green/yellow right out of the
} printer. I have read that the formulation of the ribbons may have changed
} and that the result is this sick green color.
}
} I would like to avoid swapping color/grayscale ribbons if possible but my
} users don't like the color cast to their pictures. Any ideas for a
} solution?
}
}
} Jonathan Krupp
} Microscopy and Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (408) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}




From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Tue, 16 Jan 1996 11:43:56 -0500 (EST)
Subject: Re: dye sub gray scale

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Yes, the ribbons have changed. If you complain enough and to the right
person at Kodak, Kodak will send you a disk with new color look uop
tables that can be downloaded to the printer. Your b/w prints will come
out with a slight blue tint instead of the green tint, but the blue tint
is less objectionable.
-Michael Cammer
{null signature file}

On Thu, 11 Jan 1996, Jon Krupp wrote:

} Hi:
}
} Has anyone run into a problem printing grayscale images on a Kodak/Codonics
} printer?
}
} Ours have started looking tinged with green/yellow right out of the
} printer. I have read that the formulation of the ribbons may have changed
} and that the result is this sick green color.
}
} I would like to avoid swapping color/grayscale ribbons if possible but my
} users don't like the color cast to their pictures. Any ideas for a
} solution?
}
}
} Jonathan Krupp
} Microscopy and Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (408) 459-2477
} jmkrupp-at-cats.ucsc.edu
}
}
}




From: howard-at-CSHL.org (Tamara Howard in Cold Spring Harbor Laboratory)
Date: Tue, 16 Jan 1996 14:02:16 -0500
Subject: LM - Technovit resin

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Does anyone have any experience with Technovit resin? Someone here has
heard about it, but we don't have any "real" info from users. They want to
use it for LM immuno...enzyme-based, as the tissue is massively
autofluorescent.
Thanks for the help!
Tamara Howard






From: Thomas P Schuck :      schuck-at-csd.uwm.edu
Date: Tue, 16 Jan 1996 13:16:46 -0600 (CST)
Subject: VACANCY ANNOUNCEMENT

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VACANCY ANNOUNCEMENT

UNIVERSITY OF WISCONSIN-MILWAUKEE

DIRECTOR OF ELECTRON MICROSCOPY FACILITY


The Department of Biological Sciences is seeking applications for
Director of the Electron Microscope (EM) Facility as an academic staff
appointment. Broad experience with a diversity of biological systems and
EM techniques and Ph.D. in the Biological Sciences required, postdoctoral
experience preferred. Responsibilities include: 1) management of the
Biological Science EM facility, 2) research support for and collaboration
with university faculty, 3) instruction of faculty and students in the use
of EM. An independent funded research program is desirable. The
Biological Sciences Electron Microscopy Facility consists of two
laboratories totaling 2000+ square feet, SEM and TEM scopes and support
equipment.

Applicants should submit a curriculum vitae, statement of professional
interests, and three letters of reference to:

Chairperson, EM Director Search Committee
Department of Biological Sciences
University of Wisconsin--Milwaukee
P.O. Box 413, Milwaukee, WI 53201.

The deadline for receipt of applications is February 16,1996.

Additional information about the Biological Sciences Department is
available at http:\www.uwm.edu\Dept\Biology\. The University of
Wisconsin-Milwaukee is an Equal Opportunity/Affirmative Action Employer.
Women and minority scientists are encouraged to apply for these positions.
The names of those nominees and applicants who have notrequested that
their identities be withheld and the names of finalists will be released
upon request.







From: Eric A. Rosen :      earosen-at-indirect.com
Date: Tue, 16 Jan 1996 13:36:50 +0000
Subject: disregard Forrest Gump Humor ;o)

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Message-Id: {199601162037.NAA08585-at-ns1.indirect.com}
Comments: Authenticated sender is {earosen-at-indirect.com}

Disregard the forrest gump humor I was supposed to send it to a
single person and not to the whole group} } }

Cheers ;o) :o) %o)
Eric





From: suecheng-at-codon.nih.gov (Susan Cheng)
Date: Tue, 16 Jan 1996 16:41:11 -0500
Subject: subscribe

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subscribe suecheng-at-codon.nih.gov



Susan J.-H. Tao Cheng
NIH, Bldg 36, Rm 2A-29
Bethesda, MD 20892
Tel: (301) 496 0579
Fax: (301) 402 6875





From: Thomas P Schuck :      schuck-at-csd.uwm.edu
Date: Tue, 16 Jan 1996 13:16:46 -0600 (CST)
Subject: VACANCY ANNOUNCEMENT

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VACANCY ANNOUNCEMENT

UNIVERSITY OF WISCONSIN-MILWAUKEE

DIRECTOR OF ELECTRON MICROSCOPY FACILITY


The Department of Biological Sciences is seeking applications for
Director of the Electron Microscope (EM) Facility as an academic staff
appointment. Broad experience with a diversity of biological systems and
EM techniques and Ph.D. in the Biological Sciences required, postdoctoral
experience preferred. Responsibilities include: 1) management of the
Biological Science EM facility, 2) research support for and collaboration
with university faculty, 3) instruction of faculty and students in the use
of EM. An independent funded research program is desirable. The
Biological Sciences Electron Microscopy Facility consists of two
laboratories totaling 2000+ square feet, SEM and TEM scopes and support
equipment.

Applicants should submit a curriculum vitae, statement of professional
interests, and three letters of reference to:

Chairperson, EM Director Search Committee
Department of Biological Sciences
University of Wisconsin--Milwaukee
P.O. Box 413, Milwaukee, WI 53201.

The deadline for receipt of applications is February 16,1996.

Additional information about the Biological Sciences Department is
available at http:\www.uwm.edu\Dept\Biology\. The University of
Wisconsin-Milwaukee is an Equal Opportunity/Affirmative Action Employer.
Women and minority scientists are encouraged to apply for these positions.
The names of those nominees and applicants who have notrequested that
their identities be withheld and the names of finalists will be released
upon request.







From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Tue, 16 Jan 1996 14:07:16 -0600
Subject: Re: cataracts

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MSA published an updated verion of the Safety Handbook edited by Joe
Mascorro and Vernon Barber in 1994. It is available from San Francisco
Press, Inc. Box 426800, CA 94142-6800. I believe it costs $17.00.

In our central facility, all of our electron microscopes are evaluated by
radiological professionals annually (as mandated by state law) who use
extremely sensitive detection devices. They have never detected x-rays
above background levels. Nonetheless, best to be safe and cautious.


} } On 15 Jan 1996 Hans Brinkies wrote:
} }
} } } Is there any evidence available that the extensive use of TEM's
} } } and/or SEM's may lead to the development of cataracts ?
} } }
} } }
} } } Hans Brinkies
} }
} } This is an interesting point. Some time ago someone asked a similar
} } question but I cannot remember seeing any discussion on the issue. Does
} } anyone know about the long term health effects of EM usage? Have any studies
} } been done on any aspect of this (eyesight, radiation exposure, anything else
} } I haven't thought of)?
} }
} } Ian MacLaren,
}
}
} EMSA (now MSA) published a "Handbook of X-ray safety for Electron
} Microscopists" by D.F} Parsons, V.A. Phillips, and J.S. Lally in 1973. Its
} a short pamphlet and I don't know if it is still available. A few points
} from the handbook:
}
} In regards to the need to perform an annual survey for x-ray leaks: "It
} should be noted that about 50% of those monitoring their microscopes found
} detectable leaks once or more". They reccomend film badges but I have
} never been at an institution that did this for their EM people.
}
} Under the Health Checks chapter: "An annual eye exam is desirable. Vision
} defects should be recorded and the lenses checked for opacities
} (cataracts)". No references or data supporting this statement are given.

#############################################################################
John J. Bozzola, Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Ted Boden -at-MR.SEMATECH.Org
Date: Thu, 11 Jan 1996 15:03:00 CST
Subject: here it is...

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MR-Received: by mta GATEV3; Relayed; Tue, 16 Jan 1996 11:37:37 -0600
Alternate-recipient: prohibited
Disclose-recipients: prohibited
Murphy-at-ms.sjdccd.cc.ca.us, MikeTSR-at-TXNetcom.Com
Cc: Philippe Maillot {Philippe.Maillot-at-SEMATECH.Org} ,
Carolyn Gondran {Carolyn.Gondran-at-SEMATECH.Org}
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--Boundary (ID kU5thsFKm6o0UW844dcHaQ)
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SEMATECH
2706 Montopolis Drive
Austin TX 78741


JOB TITLE: TEM Lab Technician
REQ. #: SHIFT: 21601046A/Shift 1
PAY RANGE: Tech 5-6
FLSA: Non-Exempt
DEPT. NAME: Internal Technical Support
MANAGER: Carolyn Gondran.
Tel. 512 356 3149


JOB DESCRIPTION:
Sample preparation of semiconductor materials and devices, both
plan view and cross-section, for TEM. Develop improved methods of
sample preparation. TEM operation to facilitate this development
will be encouraged. Photographic processing and filing of TEM
negatives and prints. Maintenance of sample preparation and dark
room equipment and supplies


JOB REQUIREMENTS:
2+ years experience in TEM sample preparation and photographic
processing required. Familiarity with tripod, dimpling and FIB
techniques desired. This position requires strong organizational
skills, and in particular, the ability to work on multiple tasks
while maintaining precise records and tracking procedure.
Experience in microelectronics industry a plus.

Associate degree in physics, chemistry or related subject.

--Boundary (ID kU5thsFKm6o0UW844dcHaQ)--




From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 16 Jan 1996 16:18:12 -0800
Subject: CD-R Drives

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Message-Id: {199601170016.QAA21578-at-cats.ucsc.edu}
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Content-Type: text/plain; charset="us-ascii"

Hi again,

We are considering establishing an image archiving proceedure based on
CD's. In addition the CD burner would be used by an electronic publishing
class for prototype CD's.

The choices in hardware seem to have increased a lot recently and prices
have really fallen.

If you have any sage advice concerning general guidelines or specific
choices, I would like to hear from you.

Thanks,

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
jmkrupp-at-cats.ucsc.edu






From: Eric A. Rosen :      earosen-at-indirect.com
Date: Tue, 16 Jan 1996 11:11:13 +0000
Subject: Forrest Gump Humor ;o)

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Message-Id: {199601161813.LAA18691-at-ns2.indirect.com}
Comments: Authenticated sender is {earosen-at-indirect.com}

To the list Here is something else for you to peruse through if you
are not busy and are interested in a laugh

How do you like this?? A friend mailed it to me. Its long.



FORREST in EVERYONE'S LIFE

Forrest Gump Life is like a Box of chocolates...
Forrest Dahmer People are like a box of chocolate, YUM!
Forrest Simpson Mmmmm, choolate
Forrest the Hun Chocolate all mine!
Forrest Simmons Chocolate is bad!, EXERCISE EXERCISE!
Forrest Rivera People who like Chocolate..Next on 'Forrest'
Forrest Jackson Little kids like my box of chocolates
Forrest Hefner Keep the chocolate, lose the box.
Forrest Shakespeare Chocolate, or no chocolate that's the question
Forrest Of Borg All chocolates must be assimilated
Forrest Presley Hunk a hunk of milk chocolate
Forrest Zen I am one with the chocolate
Forrest McClain I used to be a box of Chocolates
Forrest Ventura Chocolates..Alll-Riighty then...
Forrest Lauper People just wanna have chocolate
Forrest Turner What's chocolate gotta do, gotta do with it?
DR.Forrest McCoy Dammit jim, I'm a Dr., not a box of chocolate
Forrest Spock Logically speaking, we are all chocolate
Forrest Scotty The box, she's breaking apart Capt'n
Forrest Christ Let he without sin, eat the first chocolate
Forrest Rooney Why is it, that we are all chocolates?
Forrest Butler Frankly Scarlett, I don't like chocolate
Forrest O'Hara Tommorrow, is another box of chocolates.
Forrest Lee Fight with your inner chocolate
Forrest Clinton I didn't inhale the cream centers
Forrest Doo Roinks Raggy, Rocolates!
Forrest Pig Life is a box of chok-choa-che..candy
Forrest Marx That's the weirdest box of chocolates I've
ever seen....
Forrest Nicholson You want chocolate, you can't handle chocolate
Forrest Copperfield Poof, the chocolates are gone!
Forrest X We didn't land in the box of chocolate
The box of chocolate landed on us!
Forrest Hitler White Chocolates only!
Forrest the Frog Someday we'll find it,
The chocolate connections
The plain ones,
The cream filled....and me...
Forrest Eastwood I know what your thinking..
Did he eat five chocolates, or did he eat six
Well let me ask you...
Do you feel hungry PUNK?..well...DO YOU?
Forrest Barney I'm cream filled, your with nuts.
We're a box of chocoluts
Forrest Adam and Eve ADAM=Chocolates are forbidden
EVE=Just eat one....
Forrest Moses I command the chocolates to seperate!
Forrest Noah 2 creams, 2 nuts, 2 coconuts, 2 peanut butter
Forrest Ali I am the chocolate boxer!
Forrest on phonics Lief es lyk a boks uv chakolets
Forrest PsychicLine Yes, I knew you were a chocolate
1-900-FORREST oooh, can I suck your cream filled chocolates?
Forrest DatingGame Bacholer number two...
if I was a piece of chocolate..
What would you fill me with?
Forrest Alimony The Box is mine!
Forrest Adultry You just can't have just one chocolate.
The Forrest plague Ewww..these Chocolates are bad
Chief Justice Forrest Thomas I never touched her milk-duds!
Forrest Andrews The Hills are alive..like a box of chocolates
Forrest Allen Chocolate, huroof..
Forrest Costello Whose eating chocolates?
Forrest Abbot No, who is not eating chocolate
Forrest Vader Luke, I am your chocolate
Forrest Yoda There is a dark chocolate, and a light
chocolate..
Forrest Butthead Uh...life is like a box of um..chocolates
Forrest Beavis Heh heh, you said Box
Forrest Frued Is life really a box of chocolate..
or is it your mother you want?
Forrest SkeeLo I wish I had a box of Chocolates
Forrest Trebeck The answer:This is like a box of Chocolate
The Question:What is Life...
Forrest Hillary Hey Bill...those are my Chocolates!
Forrest Fudd Wife is wike a box uv chocowates
Forrest Calvin It's not a box of chocolates,
It's a transmorfgorizing ray!!
Forrest Tannen Chocolates...McFly
Dr. Forrest Ruth Chocolate is nice, but sex is better!
The Forrest Zone There is another dimension,
beyond that which is known to man,
It's a dimension of cream-filled bon-bons,
Or nutty carmel turtles,
and it lies in the white cardboard box,
in the pit of my lap.
It is a candy coated center of comparision,
And it is a place we call, The Forrest Zone.
Forrest Latin ifela sia a oxba foa hocolatesa
Forrest Lincoln Forescore and several chocolates ago,
Forrest Satan Life is a box of melted chocolates (Sign)
Forrest Churchlady Chocolates...well, isn't that special
Forrest Wayne Sh-yeah..and life is like a box of chocolates
as if...
Forrest Garth Hey mister chocolate man..whose trying to kill
you?...I don't know but her better not...
Forrest Bond Lifes a box of exploding chocolate
Forrest Smurf Smurf is a smurf of smurfs
Forrest '95 The box is the same,
The chocolates are upgraded....
Forrest Lennon Imagine there's no chocolate.
Forrest Hall Will you take the Box of Chocolates...
or what's behind CURTAIN NUMBER TWO?
Forrest Press your Luck Box of Chocolates! No Whammies...STOP!
Forrest of Fortune LIFE I_ LIKE _ __X _F CH_C_L_TE_
Forrest Popeye I yam a box of Chocolets...eg eg eg eg
Forrest Ice If you got a chocolate,
Yo I'll box it.
Check out my life,
As my D.J Rocks it!
Forrest Ross Life is a happy little box of chocolates
Forrest Bill and Ted Dude, Life's totally a box of Chocolate!
---EXCELLENT!----
___AIR GUITARS TRIUMPHANTLY_____
Forrest Fife This Box of Chocolates is a lethal weapons!
Forrest Stooges Look Chocolates....nyuk nyuk nyuk
Scram Wise guy **BOink**
Leave him ALone Moe!
Oh you want in on it too...**SLAP**/CRASH/
Forrest Reagan Life is a box of um.....
Forrest Bush This will not be another Box of Chocolates!
Forrest Limbaugh Life may be a box of Chocolates...
But the Democrats are all nuts...
Forrest COPS on location Bon bons-bon bons
whatcha going to do,
whatcha gonna do, when someone eats you!
Forrest Fued Life is like a?
Box of Chocolates...
Oh Good ANswer..Good Answer!
Box of chocolates...SURVEY SAYS?
XX|BOX OF CHOCOLATES|XX=} 54
Forrest Native Americans These chocolates are moving to a smaller box.




From: William R Oliver :      oliver-at-ipas4.afip.mil
Date: Tue, 16 Jan 1996 14:31:43 -0500 (EST)
Subject: Re: Image file conversion

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It depends on what you have and what you want. If you have a
flat line drawing in AutoCAD and just want to get it in a form
you can print out, then you just need to choose Export from the
File menu (in R13 for Windows) and export it as an EPS (encapsulated
Postscript) file. This will print directly on most Postscript printers.
You can also save it as a BMP file.

If you have a flat line drawing in a DXF file, then you will need to
convert it from a geometry file (which is what DXF is) to an image. You
can do this by loading it into AutoCAD and then exporting it as above, you
can use a file conversion program such as HiJaak Pro, or you can use one
of the DXF utilities at the Viewpoint/Avalon site I mentioned before.

If you are in AutoCAD, have a 3D object, and want to save a rendering,
then you have a couple more options. You can render in AutoCAD, either
with the native renderer or with RenderMan (I can't remember whether or
not R13 comes with RenderMan bundled or not), and save it as a BMP or EPS.
You can export the geometry file into another geometry format, load that
file into a different renderer, render there, and save the file as an
image. I, for instance, do much of my modeling in AutoCAD, and render
using 3D Studio or Lightwave 3D. There are a number of fairly good public
domain renderers as well; the best known among the folk I travel with is
POV.

If you have a geometry saved as a DXF file and want a rendered image,
remember that DXF is a *geometry* not *image* file. While, for line
drawings, many convertors will just do a simple projection (which is how
HiJaak Pro and others work), if you really want an image with shaded
graphics, you will have to make a rendering and save the image as an image
from your renderer.


On Tue, 16 Jan 1996, Alfred Kracher wrote:

} Sorry for bugging once again all those helpful people who have given advice
} on the conversion of image file formats. I specifically have a question
} about AUTOCAD drawings. If anyone knows how to convert these to more common
} image files, please get in touch with me directly.
} Thanks, Alfred
}
} Alfred Kracher
} akracher-at-iastate.edu
} http://www.public.iastate.edu/~akracher
}
}
}




From: EDWARDS-at-ssmd.mrl.dsto.defence.gov.au (Edwards, Darren)
Date: 17 Jan 1996 13:01 +0100
Subject: unsubscribe

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Message-Id: {199601170203.MAA05715-at-fang.dsto.defence.gov.au}



Unsubscribe please
==========================================================
_--_|\ D.P. Edwards [Darren.Edwards-at-dsto.defence.gov.au]
/ DSTO Ship Structures and Materials Division
\_.--._/ Defence Science & Technology Organisation
v
===========================================================









From: Brett W. Cockman :      bcockman-at-uniwa.uwa.edu.au
Date: Wed, 17 Jan 1996 12:11:02 +0700 (WAST)
Subject: Glass for Ralph knives

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X-NUPop-Charset: English

Has anybody using Ralph knives to cut resin sections, used ultramicrotome
-quality glass for making their knives? A sales person has tried to convince
me that using this quality of glass 'should' provide good knives as opposed
to 'histology - grade' glass. I pose this dilemma as I have found that
'ultramicrotome - grade' glass is locally cheaper.
Does anyone on the list have an opinion and/or advice as to a good source of
reliable glass for the Ralph knives.
Regards,

Brett Cockman




From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 17 Jan 96 08:17:25 EST
Subject: Re: Glass for Ralph knives

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Brett-
I guess I qualify as a salesperson, since we manufacture Ralph Knife Makers, and
also sell glass for them. However, I am a professional manager, not a salesman,
and I am interested primarily in selling equipment, not consumables, and it is
important to me that the equipment we sell works properly.
With that "introduction," let me give you my opinion. "Ultramicrotome glass"
and "histology glass" are labels that any vendor can put on any glass- that is,
there are no rules governing how these terms are applied. According to
conventional wisdom, "ultramicrotome glass" should be the highest quality, since
cryoultramicrotomes are much more sensitive to glass quality than are the rotary
microtomes used in histology. In our catalog, the ultramicrotome glass is of
higher quality than the histology glass, although both begin life as "float
glass" and then are cut for use in microscopy.
That all being said, there can be tremendous quality differences between glass
obtained from different sources.If you are concerened, I would recommend that
you ask your salesperson for a sample piece of glass.
Steven Slap, Vice-President
Energy Beam Sciences, Inc.





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Wed, 17 Jan 1996 10:20:52 -0600
Subject: Re: disregard Forrest Gump Humor ;o)

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Message-Id: {199601171524.JAA19206-at-BCM.TMC.EDU}
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Mime-Version: 1.0
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At 01:36 PM 1/16/96 +0000, you wrote:
} Disregard the forrest gump humor I was supposed to send it to a
} single person and not to the whole group} } }
}
} Cheers ;o) :o) %o)
} Eric
}
************************
Oh, sure we're gonna disregard it now!





From: Corvos-at-aol.com
Date: Wed, 17 Jan 1996 11:55:08 -0500
Subject: Re: disregard Forrest Gump Humor ;o)

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In a message dated 96-01-17 11:51:32 EST, you write: Yea... It is too
late.. It is now around the world with your name on it......

Walter Protheroe
E-MAC

} microscopy-at-Sparc5.Microscopy.Com






From: Ruth Hughes :      RMH-at-CIDMV1.WUSTL.EDU
Date: Wed, 17 Jan 1996 11:19:36 -0500 (CDT)
Subject: Macintosh-based software

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Hello,
I was wondering if anyone is using a Macintosh-based software for
image aquisition and manipulation that they are happy with? I have been
using Metamorph (Love it!) but now need something for the Mac.
Thanks in advance.

Ruth Hughes
Central Institute for the Deaf
818 S. Euclid
St. Louis, MO 63110
e-mail RMH-at-CIDMV1.WUSTL.EDU





From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 17 Jan 1996 13:48:43 -0500 (EST)
Subject: Amorphous Ge grids

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Does anyone have a source for amorphous Ge grids at { { $100's?
If not, does anyone know a good source for Ge--not necessarily the ultra-
pure stuff? TIA.
Yours,
Bill Tivol




From: Richard Lee :      richard_lee-at-QMGATE.ANL.GOV
Date: 17 Jan 1996 13:23:28 -0600
Subject: Used stereo/scope

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Message-ID: {n1390237174.84641-at-qmgate.anl.gov}

1/17/96
1:20 PM
Used stereo/scope

A veterinarian friend of mine has a son who wants a good stereomicroscope for
examining insects. Any offers would be appreciated under $200, especially if
it is a zoom model.





From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 17 Jan 1996 11:11:42 -0500 (EST)
Subject: Re: cataracts

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Dear Thomas,
}
} EMSA (now MSA) published a "Handbook of X-ray safety for Electron
} Microscopists" by D.F} Parsons, V.A. Phillips, and J.S. Lally in 1973. Its
} a short pamphlet and I don't know if it is still available.

It was still available about a year ago from San Francisco Press.

} A few points
} from the handbook:
}
} In regards to the need to perform an annual survey for x-ray leaks: "It
} should be noted that about 50% of those monitoring their microscopes found
} detectable leaks once or more". They reccomend film badges but I have
} never been at an institution that did this for their EM people.

We wear them routinely at the HVEM, and our users carry dosimeters
on themselves in the scope room. We have had only one incident where } ~1mr
was measured, and that was apparently not from the scope.
Yours,
Bill Tivol




From: Corvos-at-aol.com
Date: Wed, 17 Jan 1996 11:52:09 -0500
Subject: Re: equipment surveys

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All,

The amount of radiation exposure from Probes, SEMs and all, is minimum, but
it still has to be monitored by certified individuals. Most states consider
the equipment as minimumal threat devices and do not require any testing...

You must be careful, in some states like Texas and Colorado, you must be a
RSO (radiation safety officer) for that specific piece of equipment (i.e.
Cameca, JEOL, ARL, ISI, .....) to do a survey or monitor the equipment. You
must have a minimum of 5 years in repair and calibration and take a course in
radiation safety and in certain cases you must take a test.

Some other states like California, they would like you to also have
Hazwoper/Hazmat certification also, but is not required by law. It is incase
their is a lawsuit. You could be taken to the cleaners without it.

Walter Protheroe
E-MAC




From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Wed, 17 Jan 1996 14:12:41 -0500 (EST)
Subject: Re: Forrest Gump Humor ;o)

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After coming back to work and seeing this on my email, it was a welcome
treat. There were bats in the belfry from staying in the house after all
the snow we had here in Baltimore. Yes, I do know there are people out there
who will get more snow in one month than we usually get all winter. So,
thanks for your mistake.

Peace,

Phil Rutledge 8-{)





From: Nancy Desmond :      nld-at-avery.med.virginia.edu
Date: Wed, 17 Jan 1996 14:01:15 -0500
Subject: video signal averagers

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We are thinking about purchasing a Dage DSP-2000 processor for
image processing of light microscopic video images. Several
features seem useful for our images: averaging (particularly
useful), integration, and gating. It's about $6,000, and I'd
like to look at similar signal averagers for video before
ordering the Dage product. Any suggestions from readers of this
list? Thanks for your help.

--
Nancy L Desmond, Ph.D. nld-at-virginia.edu
Department of Neurosurgery 804.924.5607 (voice) 804.982.3829 (fax)
University of Virginia Health Sciences Center, Box 420
Charlottesville, VA 22908




From: slakmon-at-soquelec.com (SOQUELEC Ltd.)
Date: Wed, 17 Jan 1996 18:34:00 -0500
Subject: suscription

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Message-Id: {199601171649.RAA07123-at-gate.sbbio.be}

subscribe slakmon-at-soquelec.com
________________________________________________________________

Jean-Pierre Slakmon, Eng. Tel: (514) 482-6427
SOQUELEC Ltd. Fax: (514) 482-1929
5757 Cavendish Blvd., Suite 101
Montreal, Quebec e-mail: slakmon-at-soquelec.com
H4W 2W8, Canada http://www.cam.org/~telecom/
________________________________________________________________





From: Michael Shaffer :      mshaf-at-darkwing.uoregon.edu
Date: Wed, 17 Jan 1996 15:25:45 -0800
Subject: EPMA standards' database standard

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I'm just about to put a work study student on a project to digitize our
standards' library. Once digitized, my libray might then be put on the web
(with other libraries ???? ) But, before I do I can well imagine at least a
hundred different formats for the database entry. Has this been done
before?? Can anyone suggest the most useful format for searching the database??

cheers, shaf
{\/} /\ {\/} /\ {\/} /\ {\/} cognito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/}
Michael Shaffer, R.A. - University of Oregon Electron Probe Facility
mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu
http://darkwing.uoregon.edu/~mshaf/epmahome/





From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Wed, 17 Jan 1996 04:32:29 -0400
Subject: Re: disregard Forrest Gump Humor ;o) erroneous postings

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To those of you who are replying to people complaining about a post.
PLEASE PLEASE try and remember to post the reply to the sender and not to
the list.
Some mailers are dumb and assume the list IS the sender, but others are
not. Please check the configuration of your email software.

Many thanks.


John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html






From: waheeschen-at-dow.com (Bill Heeschen (517)636-4005 PMRC/ASL)
Date: Wed, 17 Jan 1996 14:35:26 -0500
Subject: re: Macintosh-based software

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Message-Id: {199601171936.AA14297-at-na3.dow.com}

Ruth:

The best place to start for Macintosh imaging is NIH Image. It is public
domain software from NIH and is available by anonymous FTP from:

zippy.nimh.nih.gov

There is a WWW home page, as well:

http://rsb.info.nih.gov/nih-image/

It can inherently acquire images via frame grabbers (NTSC,PAL) as well as a
host of other video-type inputs if you have the appropriate Photoshop plug-
in modules (PIM). There are also SEM/EDS interfaces (4pi analysis) for
digital acquistion using PIMs. It is able to open TIFF and PICT files
directly, any 8- or 16-bit bitmap file and "many" other formats through
import PIMs. A wide range of spatial and intensity information extraction
is available as well as image processing functions. The software is
inherently very useful and becomes extremely useful with the built-in
Pascal-like macro language, PIMs and source level programming (the Pascal
source code is public domain, as well). The manual includes a fairly
comprehensive list of commercial imaging products as well. Best of all,
getting up and running is very straightforward, particularly for someone
who has already been using imaging software. Even better than best of all
is the worldwide support network available through the NIH Image listserver.

My personal rule-of-thumb commercial value of the software would be in the
$1000 to $2000 price range, based on the feature set of NIH Image and the
comparable feature set for commercial software (Mac,Wintel, etc).

Have fun.

Bill
==========================
Bill Heeschen/Analyt. Sci.- Mat. Char. -----
1897-F Building / The Dow Chemical Co. --- ---
Midland, MI 48667 U.S.A. --- D O W ---
phone: (517)636-4005 fax: (517)636-5453 --- ---
Email: waheeschen-at-dow.com -----
========================== R




From: wong-at-msg.ucsf.edu (Mei Lie Wong)
Date: Wed, 17 Jan 1996 16:13:09 -0800
Subject: Position available

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I received this job description from someone who is not on the net. If any
one is interested all the pertinent facts are listed below.

Position description

A Research Assistant position is available in a research laboratory in the
Gerontology Division at the VA Medical Center, Palo Alto, CA. Studies
carried out in this laboratory focus on cell biology questions relating to
lipid pathways in cells and on age-related changes in cells. The position
will be available in April or May of 1996, and some overlap with the
retiring research assistant is desired. The position requires someone with
well grounded electron microscope (EM) skills with experience and an
interest in carrying out related morphological techniques including
immunocytochemistry (at the light microscope level and EM level using
cryoultramicrotomy), fluorescence microscopy, and in situ hybridization
methodology. Persons applying for this job must also have basic
biochemical skills, be adept at problem solving, flexible in working on
several projects simultaneously, and willing to learn new techniques and
use new equipment when necessary.

Employment will be through the Palo Alto Institute for Research and
Education (PRAIRIE). Salary and hours are negotiable and dependent on
training and experience. Please send resumes to Eve Reaven, PhD, VA
Medical Center, (GRECC, 182B), 3801 Miranda Ave., Palo Alto, CA 94304.
(FAX = (415) 855-9437; telephone = (415) 493-5000 x64144)

Mei Lie Wong
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
email wong-at-msg.ucsf.edu






From: Donald P Robertson :      donald-at-csd.uwm.edu
Date: Wed, 17 Jan 1996 21:44:27 -0600 (CST)
Subject: subscribe

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Subscribe microscopy Donald P. Robertson {donald-at-csd.uwm.edu}




From: carim-at-ems.psu.edu (Altaf H. Carim)
Date: Wed, 17 Jan 1996 17:22:24 -0500 (EST)
Subject: TEM - staff position

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Hello all -

If you have questions about the opening described below, contact me
by e-mail, but all application letters and resumes should be sent as
hardcopy to be considered. Note that the position involves physical
sciences and that we do not intend to consider applicants with a biological
sciences background.

--------------------------------------

TRANSMISSION ELECTRON MICROSCOPY

THE PENNSYLVANIA STATE UNIVERSITY


The Materials Characterization Laboratory at Penn State is seeking
candidates for a staff position in transmission electron microscopy. The
successful applicant will take direct responsibility for use and support of
the laboratory's 200 kV field-emission instrument, which is equipped with
EDS and PEELS detectors and a SS-CCD camera for digital acquisition. The
person selected will be expected to work with users from a variety of
disciplines on a wide range of materials problems. In addition, candidates
should be prepared to give advice on and assistance with sample
preparation, technique selection, and analysis of images, diffraction
patterns, and spectra. The person hired will also be able to pursue
collaborative or independent research as other duties allow.

Requirements include a B.S. and preferably an M.S. in Materials Science or
a related field, and additional experience beyond the degree is preferred.
The position is not intended to be a postdoctoral appointment, although
this may be considered if other suitable candidates are not found.
Candidates should have extensive experience with transmission electron
microscopy and must be skilled in one or more of the following areas:
high-resolution imaging and simulation, quantitative EDS, PEELS, electron
holography, field-emission TEM operation.

The position will be filled initially on a two-year appointment, but it is
intended that the position will be made permanent if performance is
satisfactory. Respond to Prof. A. H. Carim, 118 Steidle Building, The
Pennsylvania State University, University Park, PA, 16802.


------------------------------------------------------------------------
Prof. Altaf H. Carim tel.: (814) 863-4296
Dept. of Materials Science & Engineering fax: (814) 865-0016
118 Steidle Building e-mail: carim-at-ems.psu.edu
The Pennsylvania State University
University Park, PA 16802
------------------------------------------------------------------------






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Wed, 17 Jan 1996 21:45:51 -0800
Subject: Re: cataracts

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Dear All,
As I understand it, the concern over cataracts in an EM lab was from the UV
radiation from the evaporator, not X-rays from the EMs. The Safety in the
EM Handbook has a discussion of various eye hazards from lasers and UV
sources, but we always use a pair of welders goggles when evaporating
carbon, since the arc is dangerous to the eyes, as is a welder's arc. A few
years ago I tested my sputter coater for UV light while it was operating
and was dismayed at how much UV was coming out of the thick glass. I advise
people not to stare intently at their samples while they are coating.

A few months ago several people on the Listserver tried to see if their
physical ailments were common to other microscopists, problems such as
shoulder joint and neck pains. There were many different ailments, but no
common threads. We're all just getting older, I guess.
Hope this helps,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: jlibert-at-cpcug.org (John M. Libert)
Date: Thu, 18 Jan 1996 07:35:43 -0500
Subject: Re: Macintosh-based software

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Yes. An excellent package for color image acquisition and analysis for the
Macintosh is made by Signal Analytics. I can send you a demo disk and
literature if you are interested. I represent Signal as well as several
other software companies.

John Libert

At 11:19 AM 1/17/96 -0500, Ruth Hughes wrote:
} Hello,
} I was wondering if anyone is using a Macintosh-based software for
} image aquisition and manipulation that they are happy with? I have been
} using Metamorph (Love it!) but now need something for the Mac.
} Thanks in advance.
}
} Ruth Hughes
} Central Institute for the Deaf
} 818 S. Euclid
} St. Louis, MO 63110
} e-mail RMH-at-CIDMV1.WUSTL.EDU
}
}
}





From: davilla-at-4pi.com (Scott D. Davilla)
Date: Thu, 18 Jan 1996 07:48:03 -0600
Subject: To 4pi product users

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4pi Analysis, Inc. now has direct internet access.

For information and access see:

Web Server: http://www.4pi.com/

FTP Server: ftp.4pi.com (for software upgrades)

email: eieio-at-4pi.com (for general information)
support-at-4pi.com (for technical support)
sales-at-4pi.com (for sales information)

The old email address ( go4pi-at-applelink.apple.com) is no longer valid.

Scott D. Davilla Phone: 919 489-1757 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534







From: waheeschen-at-dow.com (Bill Heeschen (517)636-4005 PMRC/ASL)
Date: Thu, 18 Jan 1996 09:53:37 -0500
Subject: Re: video signal averagers

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Message-Id: {199601181453.AA28403-at-na3.dow.com}

Nancy:

If you are willing to use a computer as part of your video averaging
scheme, there are a number of boards which will do video-rate signal
averaging (e.g., Scion 301-695-7870, D1887-at-applelink.apple.com for Mac and
probably Wintel "soon"; Matrox 800-804-6243, imaginginfo-at-matrox.com for
Wintel). You could also consider on-chip integration, but that is camera
specific.


Bill
==========================
Bill Heeschen/Analyt. Sci.- Mat. Char. -----
1897-F Building / The Dow Chemical Co. --- ---
Midland, MI 48667 U.S.A. --- D O W ---
phone: (517)636-4005 fax: (517)636-5453 --- ---
Email: waheeschen-at-dow.com -----
========================== R




From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 18 Jan 1996 10:22:30 -0600
Subject: Forrest Gump Humor

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Message-Id: {199601181525.JAA20754-at-BCM.TMC.EDU}
X-Sender: joiner-at-bcm.tmc.edu
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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

RE: Rosen's "mistake"

Let's lighten up on the guy. A little humor...OK, long humor...is OK.

Joiner





From: James R. Stets :      stetsjr-at-ttown.apci.com
Date: Thu, 18 Jan 1996 10:42:52 -0500 (EST)
Subject: Registration and Inspection of EM's

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Regarding the thread about radiation safety and electron microscopes:

Don't assume that your state doesn't require some type of registration and
periodic inspection of your electron microscope for radiation safety. It
could cost you a hefty fine from the government agency in your state
responsible for such matters if you don't register your instruments and
have a program in place to assure compliance with the regulations.

The instrument manufacturers generally don't mention anything about the
requirements to register their instruments when you purchase and install
them. It's your responsibility to find out what your state requires.

Hope this saves somebody some headaches and some money.

Jim Stets
Air Products and Chemicals, Inc.
Allentown, PA
stetsjr-at-ttown.apci.com




From: Pal Baggethun :      MCLPALB-at-mh1.mcc.ac.uk
Date: Thu, 18 Jan 1996 20:08:13 GMT
Subject: TEM - SAD beam intensities

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TEM - SAD: Intensities of scattered beams

By measuring the intensity distribution around a Debye - ring over a
range of goniometer tilt positions I can produce a partial pole figure
of the selected specimen area. The diffracted intensity in a certain
direction is taken as the difference between the reading at the ring
and reading just inside it. The readings can be accurate to about 2
(azimuth angle).

Now, as the specimen is tilted through a decided range it becomes
necessary to make some corrections to the measured intensities since
the specimen thickness increase, as do the illuminated volume.
Absorption and extinction changes should be taken into account.

It is possible to estimate such a correction by some simple
experiments. However, what I would like to know is if there exist any
software that can calculate the needed corrections. If so, is this
calculation/software available free of charge?


Yours sincerely

P. Baggethun
Manchester Materials Science Centre
UMIST




From: philf-at-NEWTON.UMSL.EDU (Philip Fraundorf)
Date: Thu, 18 Jan 1996 14:28:25 -0600
Subject: Re[2]: some remarkable TEM facts

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Message-ID: {MAPI.Id.0016.00683536202020204437333230303030-at-MAPI.to.RFC822}
Read-Receipt-To: Jean-Louis Beek {ah56-at-solo.pipex.co.za}
Priority: Normal
To: microscopy-at-Sparc5.Microscopy.Com
MIME-Version: 1.0

Dear Bill,

You replied to:

[snip]
} } Under some illumination conditions there may be no more than 1 beam electron
} } in the column at a time!
} With our HVEM (1.2 MV) and its ~3m column this turns out to be cor-
} rect--especially since we use low-dose conditions for ED work.
Wow! The current of electrons hitting the specimen must be quite low.
How do you estimate it?

[snip]
} What are the predictions for hollow-cone illumination where we know
} the magnitude of the transverse momentum, but not its direction?
I haven't thought about it yet, but I think hollow-cone illumination was
being used by Dr. Murray Gibson's group at U. of I. (Champaign-Urbana) to
vary the coherence widths (and lengths?) of their electrons. Check with them.

I should add for listserver readers as well, that I am interested in
developing these informal observations on fast, lonely, fat and long
electrons a bit further, and of course to add new ones as well. Feedback is
invited! Some of you may see a bit more of this on paper in days ahead, but
in the meantime we're sponsoring a (hopefully ever-improving) version of the
list through our "relativity rap" web page at
{http://newton.umsl.edu/~run/rap.html} .

Cheers. /philf :)


//\/\/\/\---}
// Phil Fraundorf Physics & Astronomy/CME (314)5165044 philf-at-newton.umsl.edu
\\ B503 U.Missouri-SL St.Louis MO 63121 USA http://newton.umsl.edu/~philf
\\/\/\/\/\/\/\/---}





From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 18 Jan 1996 16:26:54 -0500 (EST)
Subject: Re: Ge grids

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} we make diamond grids. We could make amorphous Ge grids if you want.
} What do you want them for? Is there a general demand for these grids?
}
Dear Richard,
I want the grid for measuring spherical aberration from the zeros of
the contrast transfer function by Krivanek's method. I saw this described
in Ultramicroscopy 38 (1991) 225-233--which was more easily obtained than
Krivanek's paper in Optik 45 (1976) 97ff. There may be some general demand
for these grids, but I cannot tell you how much.
Yours,
Bill Tivol




From: Pal Baggethun :      MCLPALB-at-mh1.mcc.ac.uk
Date: Thu, 18 Jan 1996 22:04:36 GMT
Subject: TEM - SAD beam intensities

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TEM - SAD: Intensities of scattered beams

By measuring the intensity distribution around a Debye - ring over a
range of goniometer tilt positions I can produce a partial pole
figure
of the selected specimen area. The diffracted intensity in a certain
direction is taken as the difference between the reading at the ring
and reading just inside it. The readings can be accurate to about 2
(azimuth angle).

Now, as the specimen is tilted through a decided range it becomes
necessary to make some corrections to the measured intensities since
the specimen thickness increase, as do the illuminated volume.
Absorption and extinction changes should be taken into account.

It is possible to estimate such a correction by some simple
experiments. However, what I would like to know is if there exist any
software that can calculate the needed corrections. Is it possible by
theoretical means to evaluate this quantitatively?



Yours sincerely

Paul Baggethun
Manchester Materials Science Centre
UMIST




From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 18 Jan 1996 16:39:51 -0500 (EST)
Subject: Re: Re[2]: some remarkable TEM facts

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} [snip]
} } } Under some illumination conditions there may be no more than 1 beam electron
} } } in the column at a time!
} } With our HVEM (1.2 MV) and its ~3m column this turns out to be cor-
} } rect--especially since we use low-dose conditions for ED work.
} Wow! The current of electrons hitting the specimen must be quite low.
} How do you estimate it?
}
Dear Philip,
For the 100 micrometer C2 aperture, we get ~10^-11 amps, for the 30
micrometer C2 aperture (which is better because the spot at crossover is
~350 nm--the size of the P1 aperture) the current is too low to show up on
the screen current meter or the second Faraday cage. We made a high-precision
Faraday cage which goes on the bottom of the column, and we measure the beam
current with an electrometer.
Yours,
Bill Tivol




From: t.bostrom-at-qut.edu.au (Thor Bostrom)
Date: Fri, 19 Jan 1996 18:06:54 +1000
Subject: Re: Macintosh-based software

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About Macintosh-based software for image capture and manipulation --
We use the Prism image analysis & measurement package (Analytical Vision,
Raleigh NC) together with a RasterOps graphics display & video image capture
board on our Macintosh Quadra. We acquire color images with Adobe Photoshop.
Works well and yes we're happy with it.
(Usual disclaimer...)

Thor Bostrom

At 11:19 AM 1/17/96 -0500, Ruth Hughes wrote:
} Hello,
} I was wondering if anyone is using a Macintosh-based software for
} image aquisition and manipulation that they are happy with? I have been
} using Metamorph (Love it!) but now need something for the Mac.
} Thanks in advance.
}
} Ruth Hughes
} Central Institute for the Deaf
} 818 S. Euclid
} St. Louis, MO 63110
} e-mail RMH-at-CIDMV1.WUSTL.EDU
}
}

=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
Dr Thor Bostrom
Analytical EM Facility
Queensland University of Technology (QUT)
GPO Box 2434, Brisbane, QLD 4001, Australia
Ph: +61 7 3864-2351 FAX: +61 7 3864-5100
http://www.sci.qut.edu.au/aemf/
=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=





From: Arthur Gillman :      ARGIL-at-delphi.com
Date: Fri, 19 Jan 1996 03:02:46 -0500 (EST)
Subject: video signal averagers

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Nancy Desmond asked about signal averagers similar to the Dage DSP-2000.
Our company has been making for many years a box called the OMNEX which
is a stand-alone real time processor in some ways similar to the Dage.

The OMNEX has a lot of features. It can average, and integrate video
frames. Integration comes in 4 flavors including some automatic modes.
A unique feature is that it can control integrating cameras
for long exposure in cases of low light. It can do background subtraction,
dynamic subtraction, psuedocolor. It has a measurement section for
linear measurements, area, path, counting, stopwatch. It has a built-in
clock calender which may be displayed on the screen. It can zoom, too.
4 labels may be put on the screen. The whole thing is mouse controlled
and extremely easy to use. There is even an onscreen help facility.

Very useful: complete digital contrast enhancement including histogram
equalization and 4 custom lookup tables.

A very powerful feature is that it can do matrix operations like sharpening,
gradient, edge detection, etc. Two color overlay is also possible.

It has a section to store 4 images and sequence them like a slide show (but
up to 30 times per second.)

I am sure that there are other features that I am forgetting.

The Dage unit does not do most of these things. It is designed more for
precise control of the video parameters like black level, etc.

The OMNEX costs $5350. It is also sold by Zeiss.

I can send you a brochure if you send along your address.
Please call or Email with any questions.

We also have a very powerful video marking and measuring system called
the XR-2000.

Regards,

Arthur Gillman
Princeton, NJ





From: akracher-at-iastate.edu (Alfred Kracher)
Date: Fri, 19 Jan 1996 08:53:10 -0600
Subject: Radiation Safety

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Further on the subject of radiation safety:

When I took my legally required training for radiation protection a few
decades ago, I learned the (approximate) formula

d.r. = (0.3*(U**2)*I*Z)/r**2

where:

d.r. dose rate in [r/h] (roentgen per hour)
U accelerating voltage in [kV]
I electron beam current in [mA]
Z (mean) atomic number of the target,
r distance from the target in [cm]

Since practically all electrons that come out of the source are accelerated
to U and hit something somewhere inside the instrument, setting I equal to
the emission current (for which there is a meter on most instruments) will
give you an upper limit on the electrons that produce x-rays. Most guns are
designed for emission in the 0.1 A range. What most electrons hit is made
out of stainless steel or brass, although some also hit Pt or W apertures,
so setting Z=40 or 50 should be high enough to overestimate x-ray
production by a bunch. Assuming that it takes, say, 8mm of steel to keep a
small electron beam instrument from imploding under its own vacuum, we can
calculate that under the worst case conditions no measurable x-rays can
escape for "low voltage" work typical of SEMs. Although there are a lot of
x-rays generated, the vacuum enclosure is necessarily a very efficient
shield.

The instrument I work with, an ARL SEMQ microprobe, actually has a
half-inch steel enclosure, and its 30kV power supply cannot be cranked up
high enough to measure x-rays outside the instrument. Since we, too, have a
law requiring annual inspections, we have verified by actual measurements
under different conditions that this is true. To be on the safe side, the
manufacturer has also specified that in the case of modifications no
enclosure parts are to be made from aluminum (shielding efficiency goes
with a high power of Z).

However, the above formula also tells you that the situation quickly
changes if you use higher voltages. X-ray generation goes with the square
of U, and I typically increases as well when the voltage increases, so that
the "net" dose rate increases with something around 2.5 to 3rd power of U,
and the shielding efficiency of metal decreases as the generated x-rays get
more energetic. With 100 or 200 keV instruments, i.e. most TEMs, I would
definitely start to worry about x-ray leakage, and require people to wear
film badges or (perhaps preferably) dosimeter rings.

P.S.: Mid-Iowa temperature minus 9, wind chill index minus 60.


Alfred Kracher
akracher-at-iastate.edu
http://www.public.iastate.edu/~akracher






From: Paul Webster :      Paul.Webster-at-quickmail.yale.edu
Date: 19 Jan 1996 13:55:43 -0500
Subject: Re: cryomicrotomy

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Message :
I'm interested in suggestions for embedding media for polymeric thin films,
typically automotive coatings, which will be cryomicrotomed in the -60 to -90C
range. Most coatings will originate from a water based system.

I'm also interested in suggestions for cryomicrotomy courses which
would include bulk and embedded polymeric materials (elastomers, coatings,
alloys, and composites).

Thanks for your help.

Question :

How thin do you want the sections to be?

Paul Webster
Center for Cell imaging
Yale School of Medicine
333 Cedar Street
New Haven, CT 06520.





From: slakmon-at-soquelec.com (SOQUELEC Ltd.)
Date: Fri, 19 Jan 1996 14:57:46 -0500
Subject: ETEC SEM's service

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Would anybody know of an individual or service organization on the East
coast that services old ETEC SEM's?

Jean-Pierre Slakmon
Soquelec Limited
Montreal, Canada
________________________________________________________________

Jean-Pierre Slakmon, Eng. Tel: (514) 482-6427
SOQUELEC Ltd. Fax: (514) 482-1929
5757 Cavendish Blvd., Suite 101
Montreal, Quebec e-mail: slakmon-at-soquelec.com
H4W 2W8, Canada http://www.soquelec.com
________________________________________________________________





From: Serita Frey :      serita-at-NREL.ColoState.EDU
Date: Fri, 19 Jan 1996 14:20:59 -0700
Subject: image analysis

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I am interested in using image analysis to count bacteria, measure
fungal hyphal lengths, and estimate bacterial and fungal biovolumes in
soil samples. I am currently trying to use NIH-image for this purpose.
If you are using NIH-image or another software package for this or a
similar purpose, I would be interested in hearing from you. I am just
getting started and have a number of questions.

Thanks,

Serita Frey

------------------------------------------------------------------------
Serita Frey
Natural Resource Ecology Laboratory
Colorado State University
Ft. Collins, CO 80523
------------------------------------------------------------------------




From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 19 Jan 1996 16:40:28 -0500 (EST)
Subject: Re: Ge grids

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} This may be a naive suggestion but, wouldn't amorphous C work as well?

Dear John,
It's not a naive suggestion; however, since I am applying the
method for a 1.2 MV scope, the contrast is too low with a C film. I'm
prepared for the possibility that even a Ge film might not be quite good
enough. What I will need to do is to take an optical or computer trans-
form of the image and find the zeros of the CTF, so the better the con-
trast, the more accurate the results.
Yours,
Bill Tivol




From: Bonnie Davis - Kennametal Inc. :      bld_kmt-at-prlc.org
Date: Fri, 19 Jan 1996 14:21:21 -0500 (EST)
Subject: Job Announcement

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January 18, 1996

As part of our action-oriented programs under our Affirmative
Action Plan, this letter serves as notification that we are seeking
minority and female candidates for the following open position, but all
qualified candidates will be considered:

Senior Engineer, Mechanical Properties

- This position will be located at our Corporate Technology Center
in Latrobe, PA, working in Research and Development.

- The Sr. Engineer position requires a Masters degree, with a Ph.D.
preferred, in engineering science or materials related discipline and 2-8
years experience, dependent on the applicants degree, in the study of
structure/property relationships. Effective communication, interactive
and supervisory skills are required to work with other research personnel
and the academic community on both short and long term R&D projects.

- This responsibilities for this position are:

Utilizing TEM (STEM), SEM, EDS, and related instrumentation, provides
microstructural, physical property and mechanical property expertise on
materials including but not limited to powder and sintered, carbide,
cermet, and superhard materials.

Conducts mechanical property testing including tensile, compressive,
fracture toughness, transverse rupture and wear at room and elevated
temperatures.

Liaisons with outside organizations, universities and other laboratories
involved in joint material related research programs.

Investigates and recommends purchase of new analytical instrumentation.

Conducts validation studies of testing procedures using SPC techniques
and recommend solutions to resolve discrepancies.

Documents new and existing testing methods with specific procedures.

Represents Kennametal through technical presentations/publications,
organizational memberships, serving on committees and elected offices.

- Kennametal Inc. offers an excellent relocation and benefits
package and a salary range for this position from $3,920 per month to
$5,880 per month dependent on the candidates qualifications and experience.

All candidates for whom resumes are submitted must acknowledge
their referral source. Resumes should be sent directly to:
Kennametal Inc.
Attn: Dennis B. Harr
Manager, Human Resource Services
P.O. Box 231
Latrobe, PA 15650-0231
(412) 539-5434

Please respond ONLY to the above. DO NOT respond to the originator of
this e-mail message.




From: jlibert-at-cpcug.org (John M. Libert)
Date: Sat, 20 Jan 1996 08:09:12 -0500
Subject: Re: image analysis

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
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Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential
Microscopy ListServer {Microscopy-at-Sparc5.Microscopy.Com}
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Serita,

Please ask your questions. I would be pleased to try to answer them as would
others on the listserve.

John Libert


At 02:20 PM 1/19/96 -0700, Serita Frey wrote:
}
} I am interested in using image analysis to count bacteria, measure
} fungal hyphal lengths, and estimate bacterial and fungal biovolumes in
} soil samples. I am currently trying to use NIH-image for this purpose.
} If you are using NIH-image or another software package for this or a
} similar purpose, I would be interested in hearing from you. I am just
} getting started and have a number of questions.
}
} Thanks,
}
} Serita Frey
}
} ------------------------------------------------------------------------
} Serita Frey
} Natural Resource Ecology Laboratory
} Colorado State University
} Ft. Collins, CO 80523
} ------------------------------------------------------------------------
}
}





From: Nancy K. R. Smith :      SMITHN-at-UTHSCSA.EDU
Date: Sat, 20 Jan 1996 18:47:26 -0500 (CDT)
Subject: Update, ImageTools for Windows 95

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} Hello ImageTool Users,
}
} First we would like to thank all of you for your patience and suggestions
} for improving ImageTool. The reception for a image analysis and
} processing package for Microsoft Windows has been great. We have
} distributed over 1,000 copies world wide to major research institution
} since our initial release on August 24, 1995.
}
} Extra! Extra! ImageTool NEW RELEASE Verion 1.1
}
} Version 1.1 of ImageTool is presently available from our ftp site
} ftp://maxrad6.uthscsa.edu The new version includes all bugs reported as
} of 12/22/95 and some really nice features. We have developed a complete
} object analysis and classification plug-in. This plug-in provides for
} automated object counting and analysis with over 20 unique measurements.
} These include: area, perimeter, x, y center, feret diameter, roundness,
} compactness, major/minor axis length, slope and endpoints. Also included
} are integrated density, binary and gray centroid. Using Objects
} Classification, objects can be categorized into sub groups based on any
} of the above object measurements.
}
} Also new in version 1.1 is support for the Data Translation DT3155
} High-Accuracy Monochrome PCI Bus Frame Grabber. The driver that is
} shipping presently supports Windows NT and not Windows 95. Data
} Translation will release the Win95 driver for this acquisition board in
} February 1996.
}
} We hope that this new version will help many of you do your research.
} Please keep those suggestions of new features and any bug reports coming.
} Also please send information about how you are using ImageTool in your
} research and what features you would like to see in the next release. We
} hope to have a new release sometime in February.
}
} Thank you again for your support.
}
} S. Brent Dove
} Diagnostic Sciences
} University of Texas
} Health Science Center
} San Antonio, TX USA
} Voice: (210) 567-3333
} Fax: (210) 567-3334
} Email: dove-at-uthscsa.edu
} Web: ddsdx.uthscsa.edu
} ftp: maxrad6.uthscsa.edu
}
}
} ----------
} From: nih-image
} To: Multiple recipients of list
} Subject: Windows version
} Date: Saturday, January 20, 1996 2:36AM
}
} Does anyone know if there is a Windows version of Image, or a program
} similar.
}
} Thanks
}
} Candy
}
}
}





From: shirley.turner-at-nist.gov (Shirley Turner)
Date: Sun, 21 Jan 1996 15:09:10 -0400 (EDT)
Subject: Revised deadline for postdoctoral applications at NIST

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Due to the government furlough, the deadline for submitting applications to
NIST for postdoctoral positions has been revised as shown below.


POSTDOCTORAL POSITIONS AT NIST

The National Institute of Standards and Technology (NIST) is interested in
receiving applicants for National Research Council (NRC) postdoctoral
positions. In particular, applicants with interests in electron microscopy
are welcomed for work in the Chemical Science and Technology Laboratory.
Projects can be proposed in the fields of material science, chemistry,
mineralogy or other related fields using a variety of techniques including
high-resolution TEM, EDS, PEELS, holography, and electron energy loss
imaging, etc.


INSTRUMENTATION AVAILABLE: A Philips CM300 FEG equipped with a Gatan
Imaging Filter and a Philips CM30 with a PEELS unit are the primary TEM
instruments. There are a variety of associated instruments available
including scanning electron microscopes, secondary ion microprobes, x-ray
diffraction units, etc.

SALARY: $45,500 per year (2 year appointments)

APPLICATION (WITH DRAFT OF PROPOSAL) DUE BY: February 1, 1996

SUPPORTING DOCUMENTS AND FINAL PROPOSAL DUE BY: February 15, 1996

EXPECTED STARTING DATE: Fall 1996 (PhD must be complete by this time)

FOR MORE INFORMATION: contact Eric Steel (301) 975-3902,
eric.steel-at-nist.gov or Shirley Turner (301) 975-3923, shirley.turner-at-nist.gov.

NOTE: US Citizenship is required.





From: Keith Moulding :      mcmouldk-at-uxmail.ust.hk
Date: Mon, 22 Jan 1996 09:24:33 +0800
Subject: TEM Cameras

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Hello,

I am looking for information on a company which I believe is called AMT. I
have been told they make interface flanges for cameras on TEMs. In
particular, I need to connect a camera to a 100CXII - either below the
camera chamber or on the 35 mm port, and to a CM20 below the camera chamber.

The cameras will not be used for high resolution work, but for training
purposes.

If anybody knows of the company or any other sources please let me know.

Keith Moulding.

Materials Characteristion Preparation Centre,
Hong Kong Univeristy of Science and Technology,

e-mail: mcmouldk-at-uxmail.ust.hk






From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Mon, 22 Jan 1996 08:10:55 +0000 (GMT)
Subject: Re: cryomicrotomy

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In reply to Paul Webster from Yale about any Cryomicrotomy Courses.
Suggest contacting David Remsen on e-Mail address
dremsen-at-aaem.amc.anl.gov who is organizing a course on Cryomicroscopy
and related techniques at Woods Hole MBL May 29-June5 1996.

Patrick Echlion
Multi-Imaging Centre
Cambridge University

On 19 Jan 1996,
Paul Webster wrote:

} Message :
} I'm interested in suggestions for embedding media for polymeric thin films,
} typically automotive coatings, which will be cryomicrotomed in the -60 to -90C
} range. Most coatings will originate from a water based system.
}
} I'm also interested in suggestions for cryomicrotomy courses which
} would include bulk and embedded polymeric materials (elastomers, coatings,
} alloys, and composites).
}
} Thanks for your help.
}
} Question :
}
} How thin do you want the sections to be?
}
} Paul Webster
} Center for Cell imaging
} Yale School of Medicine
} 333 Cedar Street
} New Haven, CT 06520.
}
}




From: luciom-at-NEWTON.UMSL.EDU (lucio MuleStagno)
Date: Fri, 19 Jan 1996 18:00:18 -0600
Subject: Image analysis : Image-Pro Plus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
I am considering buying Image-Pro plus software to do some of the
things a lot of you have mentioned in the past : feature counting on
prints/images, digitalization of negatives, image enhancement and printing.

The system we are thinking of will be attached to a Nomarski
microscope for optical work ( defect counting of etched wafer surfaces ),
and to a seperate camera mounted on a light-box to digitalize negatives and
prints. We have not made a final decision on :
1. resolution of cameras to buy.
2. black and white vs. color.
3. type of printer/s to have available ( the company suggests a Kodak
for high quality work )

Anyone out there using a similar setup to count/size and digitalize ?
Anyone using Image Pro Plus ?

thanks

Lucio


***********************************************************************
Lucio Mule'Stagno
Physics Dept & Center for molecular electronics
Univ. of Missouri- St.Louis
tel 314 - 516 5933
fax : 314-516 6152
e- mail LUCIOM-at-NEWTON.UMSL.EDU

MEMC Electronic Materials Inc.,
Materials Technology Grp.,
St.Peters,MO
tel 314 279-5000 ext 2315
fax 314 279 5157
e-mail LMULESTAGNO-at-MEMC.COM

************************************************************************

" War is over.. If you want it " Give Peace a Chance, John Lennon


*************************************************************************





From: luciom-at-NEWTON.UMSL.EDU (lucio MuleStagno)
Date: Fri, 19 Jan 1996 18:00:18 -0600
Subject: Image analysis : Image-Pro Plus

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hi all,
I am considering buying Image-Pro plus software to do some of the
things a lot of you have mentioned in the past : feature counting on
prints/images, digitalization of negatives, image enhancement and printing.

The system we are thinking of will be attached to a Nomarski
microscope for optical work ( defect counting of etched wafer surfaces ),
and to a seperate camera mounted on a light-box to digitalize negatives and
prints. We have not made a final decision on :
1. resolution of cameras to buy.
2. black and white vs. color.
3. type of printer/s to have available ( the company suggests a Kodak
for high quality work )

Anyone out there using a similar setup to count/size and digitalize ?
Anyone using Image Pro Plus ?

thanks

Lucio


***********************************************************************
Lucio Mule'Stagno
Physics Dept & Center for molecular electronics
Univ. of Missouri- St.Louis
tel 314 - 516 5933
fax : 314-516 6152
e- mail LUCIOM-at-NEWTON.UMSL.EDU

MEMC Electronic Materials Inc.,
Materials Technology Grp.,
St.Peters,MO
tel 314 279-5000 ext 2315
fax 314 279 5157
e-mail LMULESTAGNO-at-MEMC.COM

************************************************************************

" War is over.. If you want it " Give Peace a Chance, John Lennon


*************************************************************************





From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 22 Jan 96 11:01:27 EST
Subject: TEM Cameras

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Message-id: {23928743-at-dancer.Dartmouth.EDU}

It has been my experience that a camera mounted below the camera chamber
subjects the final image to a magnification of at least twenty (20)times that
of a film.If this is not desired I suggest you install the camera above the
camera chamber where the magnification is a more reasonable five (5) times.
Kate Connolly




From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Mon, 22 Jan 1996 08:10:55 +0000 (GMT)
Subject: Re: cryomicrotomy

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199601221447.JAA04642-at-hoh.mbl.edu}

In reply to Paul Webster from Yale about any Cryomicrotomy Courses.
Suggest contacting David Remsen on e-Mail address
dremsen-at-aaem.amc.anl.gov who is organizing a course on Cryomicroscopy
and related techniques at Woods Hole MBL May 29-June5 1996.

Patrick Echlion
Multi-Imaging Centre
Cambridge University

On 19 Jan 1996,
Paul Webster wrote:

} Message :
} I'm interested in suggestions for embedding media for polymeric thin
films,
} typically automotive coatings, which will be cryomicrotomed in the -60
to -90C
} range. Most coatings will originate from a water based system.
}
} I'm also interested in suggestions for cryomicrotomy courses which
} would include bulk and embedded polymeric materials (elastomers,
coatings,
} alloys, and composites).
}
} Thanks for your help.
}
} Question :
}
} How thin do you want the sections to be?
}
} Paul Webster
} Center for Cell imaging
} Yale School of Medicine
} 333 Cedar Street
} New Haven, CT 06520.
}
}


------- End of Forwarded Message





From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Mon, 22 Jan 1996 18:20:49 +0000 (GMT)
Subject: EELS analyser makers

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Dear all,

I am looking for information about EELS analyser makers, for
implementation on a 300 KV TEM. I heard of Gatan analyser or image
filtering system, but would like to know if there are any other makers
providing this kind of apparatus.

Thank you in advance for providing any fax number or E-mail address,

Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Luis Sole i Sabaris
E-08028 BARCELONA

Tel +34 3 402 16 95
Fax +34 3 402 13 98




From: allardlfjr-at-ornl.gov (Larry Allard)
Date: Mon, 22 Jan 1996 13:41:20 -0500
Subject: TEM Cameras

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Regarding magnification: The Gatan camera mounted below our HF-2000 and
our 4000EX instruments has a magnification factor over the film of 1.44
times the width of the displayed image in inches (since the chip is about 1
inch on a side). For an image displayed on a hard-copy output that is say
7 inches wide, this give a final image magnification of 10 times the
microscope magnification.

Larry Allard






From: Paul Webster :      Paul.Webster-at-quickmail.yale.edu
Date: 22 Jan 1996 15:04:33 -0500
Subject: Correction Re- cryomicrotom

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Message-Id: {n1389799217.90988-at-QuickMail.Yale.edu}

May I also add to this that I was not the one requesting information about
cryomicrotomy or courses. I was responding to an anonymous contributor from
Dupont (ratyrg-at-ESVAX.DNET.DUPONT.COM) including the original message with my
question.
Since then we have had a short discussion on the subject away from the BBS.







From: MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Mon, 22 Jan 1996 10:16:26 +0800PST
Subject: NIH image and Mac

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Someone in our depeartment would like to know what type of Mac they are
using, or would recommend to use, to run NIH-Image?? They are typically
dealing with images that are 1kbx1kb 24 bit colour. They are
contemplating buying a new Mac to run Image and would like input as to
what people are using and how well they run Image before buying a new
computer. Any and all comments would be appreciated.

Mark Elliott
Research Associate
UBC-Pulmonary Research Lab





From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Mon, 22 Jan 1996 17:16:19 -0500
Subject: Re: Image analysis : Image-Pro Plus

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Detection of features in Nomarski (DIC) images can be tricky. Although
your eye will see them, thresholding may be difficult because of the
"shadowing effect" that DIC has. Even selection by color can be
difficult. I had poor results thresholding transmitted light DIC
(biological); better results with reflected light DIC (ceramics).

We use Optimas, an alternative to Image-Pro for Windows based image
processing (color and b/w). You should also do a serious evaluation of
frame grabber boards, including how well they work with your I.A. system.
Our first system has an Imaging Technology CFG board, which was pricey but
worked well. Our second system has a Coreco TCX, which has some timing
delays with Optimas.

No commercial endorsement should be implied.

David Rothbard

} Hi all,
} I am considering buying Image-Pro plus software to do some of the
} things a lot of you have mentioned in the past : feature counting on
} prints/images, digitalization of negatives, image enhancement and printing.
}
} The system we are thinking of will be attached to a Nomarski
} microscope for optical work ( defect counting of etched wafer surfaces ),
} and to a seperate camera mounted on a light-box to digitalize negatives and
} prints. We have not made a final decision on :
} 1. resolution of cameras to buy.
} 2. black and white vs. color.
} 3. type of printer/s to have available ( the company suggests a Kodak
} for high quality work )
}
} Anyone out there using a similar setup to count/size and digitalize ?
} Anyone using Image Pro Plus ?
}
} thanks
}
} Lucio
}

--
Institute of Paper Science and Technology






From: Xinyang Li :      xyl-at-uow.edu.au
Date: Tue, 23 Jan 1996 10:35:15 +1100 (EST)
Subject: subscribe

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subscribe




From: Silvia Moreno :      smoreno-at-kinase.uba.ar
Date: Mon, 22 Jan 1996 20:29:13 ARG
Subject: Help

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I'm at present trying to visualize through TEM an atypical form of growth
of a dimorphic fungus, Mucor rouxii, which under normal conditions grows
as mycelia and when cAMP analogs are added to the growth media grows iso-
diametrically; germ tube emission is impaired, but growth is not impaired.
Cells , under these growth conditions are very fragile, and even tend to explode
when being under a coverslip. The cell wall is several times wider than the
normal cell wall. The microscopic preparations for TEM are not good; the cell
looks completely collapsed and detached from the wall. We have already tried
adding sorbitol 0.5 M and KCl 0.6 M at the end of the growth period, before
isolating the cells, with no success. Can someone give me a suggestion, or
indicate me a reference . We obviously are no experts in electronic microscopic
observations.
Thank you very much.
--
Silvia Moreno
smoreno-at-kinase.uba.ar




From: shayashi-at-opt.olympus.co.jp (Shinichi Hayashi)
Date: Tue, 23 Jan 1996 19:48:06 +0900
Subject: Inquiry

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To all;

Does anyone in this list know the e-mail address or the FAX number of prof.
Ernst H. K. Stelzer at European Molecular Biology Lab?
Please reply to me directly. Thanks!

-------------------------------
Shinichi Hayashi $B!JNS!!??;T!K(B
Optical R&D 2nd Group
Olympus Optical Co., Ltd.
2951 Ishikawa-cho
Hachioji, Tokyo 192
Tel: (81) 426-42-5007
Fax: (81) 426-42-2102
e-mail: shayashi-at-opt.olympus.co.jp





From: ROBIN CROSS :      EURC-at-giraffe.ru.ac.za
Date: Tue, 23 Jan 1996 12:41:37 GMT+0200
Subject: cataracts

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About a year ago, after using EM's for 27 years and having
developed cataracts in both eyes at an unusually early age
(49), I asked the question through this medium about eye damage
resulting from long-term use of electron microscopes. As in the
current discussion some interesting comments were made. There is
definitely evidence that radiation from a variety of sources
causes cataracts, typically in the posterior capsule of the lens
- not the nucleus where senile cataracts usually develop.
However, modern electron microscopes should be adequately
screened to prevent this. In my case the cataracts were
apparently typical of radiation-induced cataracts although when
and where the irradiation took place is anyone's guess. Someone
has mentioned UV radiation from vacuum evaporators. This could
be a possibility because I know of little in the way of
precautions which are routinely taken to prevent this.



Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)




From: King :      King-at-bioscience.biology.utah.edu
Date: 23 Jan 1996 07:50:34 U
Subject: Charging for Services

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Message-ID: {n1389739019.47591-at-bioscience.utah.edu}

Subject: Time: 7:20 AM
Charging for Services Date: 1/23/96

This request is made in the context of a U.S. university laboratory run as a
'recharge center' with a 'revolving account' where most of the users are
funded by NSF or NIH. The laboratory provides a range of services in light
and electron microscopy for researchers from several departments. Service
contract costs for the instruments are recovered through hourly usage
charges. Consumable supplies are charged at replacement cost or are replaced
'in kind' by the users. Other services are provided at cost. The university
requires that, over time, the account will not make a profit and will not
carry a continued balance greater than 10% of the operating costs of the
facility.

There has been increasing interest in devising a system whereby users could
be charged a fixed sum that would provide 'unlimited' access to one or more
instruments/services for a specified time. My understanding is that such a
system would violate granting agency prohibitions against prepayment for
services, and would similarly violate current university regulations
governing recharge centers.

I am interested, therefore, in hearing from anyone who has a system in place
that provides in some way for 'lump sum' payments for instrument
access/laboratory services. Since the university's recharge regulations will
be revised this year, I would also like to hear from anyone whose laboratory
appears to operate under less stringent university regulation.

Thank you.

Edward J. King
king-at-bioscience.utah.edu
Department of Biology
University of Utah






From: Joe D Geller :      geller-at-world.std.com
Date: Tue, 23 Jan 1996 10:22:31 +0001 (EST)
Subject: EDS algorithms

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We are looking for algorithms to process EDS spectra. The algorithms
should include background subtration and calculation of k-ratios from
unknown specimens (standardless analysis). Any information would be
very helpfull.

Joe Geller
Geller MicroAnalytical Lab.
426e Boston St.
Topsfield, Ma 01983-1212
508 887-7000
fax 887-6671




From: emccjb-at-ttuhsc.edu (Charles J. Butterick)
Date: Tue, 23 Jan 1996 09:58:41 -0600
Subject: EM Tech Position

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The Electron Microscopy Center at Texas Tech University Health Sciences
Center has an EM Technician II position open.
Basic requirements are a bachelor's degree and one year experience with
EM. The bachelor's degree can be substituted with an associate's degree
from a 2 year EM program or a MSA accreditation and 3 years of experience.
Any combination of experience in EDXA, morphometry, image analysis, light
microscopy, confocal, histology, and/or flow cytometry would be a plus.
Please reply before January 31, 1996 to: TTUHSC Office of Human
Resources, 3601 4th Street, Lubbock, Texas 79430
TTUHSC IS AN EEO/AA EMPLOYER



Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: waheeschen-at-dow.com (Bill Heeschen 517-636-4005 Materials/ASL)
Date: Tue, 23 Jan 1996 10:47:34 -0500
Subject: Re: NIH image and Mac

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Message-Id: {199601231547.AA10606-at-na3.dow.com}


The PowerMacs (PPC601 or 604 based) are generically the best way to go for
running NIH Image. I have been using the 7500 (which is a PCI-bus based
system) and the PCI Scion LG-3 frame grabber (PAL version). The PCI
version is very fast for video capture, although it sounds like your user
has an alternate source of images. (Also, they need to be aware that NIH-
Image is not a true 24-bit color package - It handles the three color
planes as a stack of 8-bit images. They should consider something like IP-
lab Spectrum for true-color quantification or Photoshop for "graphic arts
type" image massaging and printing.) I have also used the built-in video
on the 7500. It is a reasonable image quality, but lacks the "scientific"
capabilities that the Scion has (grayscale quality, gain/level adjust,
analog out, digital I/O, etc.).

The 7500/8500/9500 PowerMacs all have high-speed internal SCSI, so disk I/O
is very good. The 7500 is probably the best "bang/buck" system right now.
It also uses a daughterboard processor card, so it can be upgraded to a PPC
604, in principal. If I had the budget and had to spend it right now, I
would look very hard at the 132 MHz 9500. Of course, this family has now
been out long enough that it is likely Apple will introduce a new even-
faster Mac family soon. My impression has been that the best value has
been on the model which is two steps back and within the same family as the
most-recent top model (Current model example: high end is the 9500, hence
the 7500 is the best value - i.e., affordable on tight budget, but
excellent performance. Most recent past example: the 950 high-end/650 best
buy). The clones have some VERY IMPRESSIVE specifications, (combination
PCI/NuBus, high processor speed, etc) but may require optional hardware
that is already in the Mac, depending on your use environment.

Hope this helps

Bill
==========================
Bill Heeschen/Analyt. Sci.- Mat. Char. -----
1897-F Building / The Dow Chemical Co. --- ---
Midland, MI 48667 U.S.A. --- D O W ---
phone: (517)636-4005 fax: (517)636-5453 --- ---
Email: waheeschen-at-dow.com -----
========================== R




From: Tina Carvalho :      tina-at-halia.pbrc.Hawaii.Edu
Date: Tue, 23 Jan 1996 08:55:14 -1000 (HST)
Subject: Re: Charging for Services

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As I am in one of the facilities who used to have a "pre-paid, lump-sum"
system in place that appeared to make everybody happy, only to have a
federal audit come in and SEVERLY penalize us for doing so, I would also
be interested in what other recharge facilities are doing. Please keep
this dialog going, or post a compilation of replies!

Mahalo,
Tina

*****************************************
Tina (Weatherby) Carvalho *
Biological Electron Microscope Facility *
University of Hawaii *
(808) 956-6251 *
tina-at-ahi.pbrc.hawaii.edu *
http://www.pbrc.hawaii.edu/bemf/ *
*****************************************





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 23 Jan 1996 14:18:17 -0400
Subject: RE-EDS Algorithms

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Message-ID: {n1389715487.38368-at-mse.engin.umich.edu}

Subject: Time: 2:09 PM
OFFICE MEMO RE:EDS Algorithms Date: 1/23/96

Years ago John Russ published a couple of dozen algorithms for carrying out
various tasks involved in processing EDS spectra in the "EDAX EDITor", a
publication of the EDAX Company. Although these are now several years old,
they still might be useful as a starting point, and are certainly of some
historical interest. If you are interested in them, John can probably help
you with this; otherwise, I still have copies of a number of issues of the
EDAX EDITor, and could let you borrow them.
W. C. Bigelow (bigelow-at-umich.edu)





From: huffe-at-pgL7.chem.nyu.edu (Edward J. Huff)
Date: Tue, 23 Jan 1996 16:46:06 -0500
Subject: Re: NIH image and Mac

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Look at the nih-image web site, http://rsb.info.nih.gov/nih-image/
which you can also find using "Net Search" for "nih image", in your
favorite web browser like Netscape.

This is an interesting web site even if you don't want to use NIH Image.

(NIH Image runs on power macs as well as the 68k macs).




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Tue, 23 Jan 1996 10:47:55 EST
Subject: cryomicrotomy of polymer thin films

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

Someone wrote recently:

"I'm interested in suggestions for embedding media for polymeric thin
films, typically automotive coatings, which will be cryomicrotomed in
the -60 to -90C range. Most coatings will originate from a water
based system"

Could you be more specific as to whether you are interested in the
dispersion of additives within the thin film (of which there are
typically high loadings in many "automotive" coatings) or if you are
interested in the air interface surface detail. In either case, you do
have to think about "passivating" the top (and bottom) surfaces in
order to keep the embedding resin, what ever it is you eventually use,
from possibly swelling or other wise changing in some subtle way the
polymer layer of interest. We have found that a thin film of
sputtered gold works fine, Pt slightly better. For the opposite size,
we like to coat with Al so that there is never any question as to which
side is which once in the TEM.

If interested primarily in the dispersion of additives in the layer,
then the best approach is to embed completely the now both-side
passivated film and section.

If interested primarily in the top layer, we "back embed" only (e.g.
the bottom surface but after Al coating), gold sputter the top surface
but do not embed the top surface. That way, by following the gold layer
one can fairly easily discriminate between fact and artifact, that is,
surface disruption that was there to begin with vs. surface disruption
caused by the knife. We find that additive particles tend to be more
likely to be pulled out of the section when using this variation of the
approach.

We have found overall several of the so-called "Epon substitutes" are
ideal for this particular application, such as our own "SPI-Pon 812"
resin. Since the hardness of the final block has to be made to reflect
the "hardness" of inorganic additives, if present, the "Epon 812" type
systems permit what we find to be the very widest latitude in terms of
hardness levels. The fact the system was once "water based" should not
make any difference in terms of your choice of embedding system since
by the time the resin "sees" the sample, any water has long since
disappeared. If your sections do not come out perfect that first time,
then varying the hardness of the block might be the first next thing
you would want to try. But don't lose confidence in the resin system.

And assuming you are the cost conscious type, the "Epon 812 substitute"
resins are about the lowest cost of any resin.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Wed, 24 Jan 1996 10:27:34 +1100
Subject: SEM - free ETEC filaments

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X-Sender: st004718-at-brandywine.otago.ac.nz
Message-Id: {v01530500ad2b1d5ff373-at-[139.80.120.183]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


We have the following 23 filaments which were purchased for an ETEC
Autoscan, they are free to anyone who would like them for an ETEC or
similar instrument:
"EBTEC rebuilt filaments for ETEC, MR 73" (Probing and Structure) - 13 filaments
"VL-EO-R" (Energy Beam Sciences) - 10 filaments

Regards



Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD















From: H.BRINKIES -SE108/TEL.8657 :      hbrinkies-at-swin.edu.au
Date: Wed, 24 Jan 1996 10:39:29 EST+10
Subject: OrtecEEDS-II

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Message-Id: {199601232339.AA19555-at-lucy.swin.edu.au}
Comments: Authenticated sender is {hbrinkies-at-gpo.swin.edu.au}

This message might be mainly of interest to the Australian EM community,
however, I did not receive any reply to my advertisement in the latest
Australian EM Newsletter (48).

So let's try again:

For sale (very cheap). Preloved ED-System. Ortec EEDS-II (needs
attention). Consisting of Si(Li) horizontal detector (refurbished by
HNU Systems in 1993). Two consoles: Mark I (1981) + Mark II (1983).
Epson Printer. Software on 8" floppies, circuit diagrams, manuals,
spare 8" disk drives, several spare boards. Best offer accepted.

Hans G Brinkies
SWINBURNE, University of Technology
Mechnical and Manufacturing Engineering
P.O.Box 218 - HAWTHORN, 3122 - Australia
Phone: + 61 3 9214 8657 Fax: + 61 3 9214 8264




From: emccjb-at-ttuhsc.edu (Charles J. Butterick)
Date: Tue, 23 Jan 1996 13:39:20 -0600
Subject: EM Technician Position

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The Electron Microscopy Center at Texas Tech University Health Sciences
Center has an EM Technician II position open. Basic requirements are a
bachelor's degree and one years experience with EM. The bachelor's degree
can be substituted with an associate's degree from a 2 year EM program or a
MSA accreditation and 3 years of experience. Any combination of experience
in EDXA, morphometry, image analysis, light microscopy, histology, and/or
flow cytometry would be a plus. Please reply before January 31, 1996 to:
TTUHSC Office of Human Resources, 3601 4th Street, Lubbock, Texas 79430
TTUHSC IS AN EEO/AA EMPLOYER



Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: dbd1-at-uclink4.berkeley.edu
Date: Tue, 23 Jan 1996 09:31:09 -0800
Subject: email address change

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microscopy-at-aaem.amc.anl.gov, sbenson-at-darwin.sci.csuhayward.edu,
102700.1337-at-CompuServe.COM, bischof-at-maroon.tc.umn.edu,
susan_brady-at-maillink.berkeley.edu, view-at-nature.berkeley.edu,
tina-at-ahi.pbrc.hawaii.edu, csencits-at-aaem.amc.anl.gov,
sdangelo-at-cgl.ucsf.edu, DAVIS_JON/HP4700_M1-at-hpesf.cs.itc.hp.com,
mrdunlap-at-ucdavis.edu, richard.easingwood-at-stonebow.otago.ac.nz,
Paul.Feigenbaum-at-ncal.kaiperm.org, dougflyfsh-at-aol.com,
efujikawa-at-idec.com, garcar-at-nature.berkeley.edu,
jgilkey-at-ccit.arizona.edu, barbara-at-are.berkeley.edu,
lhalvson-at-nature.berkeley.edu, phamil-at-uclink2.berkeley.edu,
fahayes-at-ucdavis.edu, helena-at-nature.berkeley.edu,
trish-at-nature.berkeley.edu, holt-at-bmt.ca.boeing.com,
andyoj-at-uclink.berkeley.edu, labequip-at-aol.com,
ckeith-at-biosci.mbp.missouri.edu, erol-at-uclink2.berkeley.edu,
lkerr-at-mbl.edu, sgkcck-at-aol.com, knoff-at-lipovx.lbl.gov, jkolk-at-daystar.com,
emlab-at-ucsco.ucsc.edu, skurland-at-gatan.com, dlapidus-at-uclink.berkeley.edu,
mdlaws-at-cmsa.berkeley.edu, listserver-at-aaem.amc.anl.gov, mitch-at-meq.com,
murphy-at-ms.sjdccd.cc.ca.us, bnordhau-at-cvdls-e201.ucdavis.edu,
dpardoe-at-darwin.sci.csuhayward.edu, gpoinar-at-nature.berkeley.edu,
peiqi-at-mendel.berkeley.edu, lab_shatz-at-maillink.berkeley.edu,
stever-at-mendel.berkeley.edu, grogers-at-uclink4.berkeley.edu,
ruzin-at-nature.berkeley.edu, David_Sanan.GICD-at-quickmail.ucsf.edu,
blay-at-pondside.uchicago.edu, jerk-at-uclink.berkeley.edu,
schulzp-at-alm.usf.ca.edu, shima1-at-levi.com, shimeta-at-violet.berkeley.edu,
mwsiegel-at-gene.com, nsmith-at-darwin.sci.csuhayward.edu,
steiger-at-LCBVAX.cchem.berkeley.edu, tibbetts-at-uclink.berkeley.edu,
ltravis-at-semi.org, frits-at-cse.ucsc.edu, jeffv-at-dnai.com,
karenw-at-ucmp1.berkeley.edu, wilt-at-garnet.berkeley.edu, wong-at-msg.ucsf.edu,
dwray-at-indra.com, tyasumura-at-vines.colostate.edu

If you already have ben notified, ignore this.

If not, please update your e-mail address list with my new location:

dbd1-at-uclink4.berkeley.edu

Thank y'all.

Doug Davis
EML Berkeley


=F8=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=
=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=F8
=F8 Doug Davis =F8
=F8 Staff Research Associate =F8
=F8 Electron Microscope Facility =F8
=F8 University of California =F8
=F8 Berkeley, CA 94720 =F8
=F8 (510) 642-2085 =F8
=F8 dbd1-at-uclink4.berkeley.edu =F8
=F8=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=
=B5=B5=B5=B5=B5=B5=B5=B5=B5=B5=F8






From: dbd1-at-uclink4.berkeley.edu
Date: Tue, 23 Jan 1996 14:40:07 -0800
Subject: email address change

Contents Retrieved from Microscopy Listserver Archives
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microscopy-at-aaem.amc.anl.gov, sbenson-at-darwin.sci.csuhayward.edu,
102700.1337-at-CompuServe.COM, bischof-at-maroon.tc.umn.edu,
susan_brady-at-maillink.berkeley.edu, view-at-nature.berkeley.edu,
tina-at-ahi.pbrc.hawaii.edu, DAVIS_JON/HP4700_M1-at-hpesf.cs.itc.hp.com,
mrdunlap-at-ucdavis.edu, richard.easingwood-at-stonebow.otago.ac.nz,
Paul.Feigenbaum-at-ncal.kaiperm.org, dougflyfsh-at-aol.com,
efujikawa-at-idec.com, garcar-at-nature.berkeley.edu,
jgilkey-at-ccit.arizona.edu, barbara-at-are.berkeley.edu,
lhalvson-at-nature.berkeley.edu, phamil-at-uclink2.berkeley.edu,
fahayes-at-ucdavis.edu, helena-at-nature.berkeley.edu,
trish-at-nature.berkeley.edu, holt-at-bmt.ca.boeing.com,
andyoj-at-uclink.berkeley.edu, labequip-at-aol.com,
ckeith-at-biosci.mbp.missouri.edu, erol-at-uclink2.berkeley.edu,
lkerr-at-mbl.edu, sgkcck-at-aol.com, knoff-at-lipovx.lbl.gov, jkolk-at-daystar.com,
emlab-at-ucsco.ucsc.edu, skurland-at-gatan.com, dlapidus-at-uclink.berkeley.edu,
mdlaws-at-cmsa.berkeley.edu, listserver-at-aaem.amc.anl.gov, mitch-at-meq.com,
murphy-at-ms.sjdccd.cc.ca.us, bnordhau-at-cvdls-e201.ucdavis.edu,
dpardoe-at-darwin.sci.csuhayward.edu, gpoinar-at-nature.berkeley.edu,
peiqi-at-mendel.berkeley.edu, lab_shatz-at-maillink.berkeley.edu,
stever-at-mendel.berkeley.edu, grogers-at-uclink4.berkeley.edu,
ruzin-at-nature.berkeley.edu, David_Sanan.GICD-at-quickmail.ucsf.edu,
blay-at-pondside.uchicago.edu, jerk-at-uclink.berkeley.edu,
schulzp-at-alm.usf.ca.edu, shima1-at-levi.com, shimeta-at-violet.berkeley.edu,
mwsiegel-at-gene.com, nsmith-at-darwin.sci.csuhayward.edu,
steiger-at-LCBVAX.cchem.berkeley.edu, tibbetts-at-uclink.berkeley.edu,
ltravis-at-semi.org, frits-at-cse.ucsc.edu, jeffv-at-dnai.com,
karenw-at-ucmp1.berkeley.edu, wilt-at-garnet.berkeley.edu, wong-at-msg.ucsf.edu,
dwray-at-indra.com, tyasumura-at-vines.colostate.edu

If you already have ben notified, ignore this.

If not, please update your e-mail address list with my new location:

dbd1-at-uclink4.berkeley.edu

Thank y'all.

Doug Davis
EML Berkeley


=3DF8=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=
=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3D
=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DF8
=3DF8 Doug Davis =3DF8
=3DF8 Staff Research Associate =3DF8
=3DF8 Electron Microscope Facility =3DF8
=3DF8 University of California =3DF8
=3DF8 Berkeley, CA 94720 =3DF8
=3DF8 (510) 642-2085 =3DF8
=3DF8 dbd1-at-uclink4.berkeley.edu =3DF8
=3DF8=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=
=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3D
=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DB5=3DF8






From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 23 Jan 1996 22:52:54 -0600
Subject: Re: TEM - SAD beam intensities

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Pal & others

To do this experiment correctly you must energy filter
the diffraction pattern to correctly determine
the diffracted intensities. This filtering removes all the inelastic
scattering. It is not sufficient to simply measure the
intensity just inside the ring to correct for "background". You had best check
the literature before doing alot more work.

There are some very good papers by David Cockayne & Colleagues
at the University of Sydney. Circa late 1980's in Acta Crys.

I would suggest you look them up before continuing.


Nestor

Your Friendly Neighborhood SysOp.






From: philippe.buffat-at-cime.uhd.epfl.ch (Philippe-Andre Buffat)
Date: Wed, 24 Jan 1996 08:59:03 +0100
Subject: Winter School/Diffraction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-ID: {MAPI.Id.0016.00683536202020204434454530303237-at-MAPI.to.RFC822}
Read-Receipt-To: Jean-Louis Beek {ah56-at-solo.pipex.co.za}
Priority: Normal
To: microscopy-at-Sparc5.Microscopy.Com
MIME-Version: 1.0

The Association Vaudoise des Chercheurs en Physique organizes a

**** Winter School****

M=E9thodes modernes de diffusion et de diffraction des neutrons, =E9lectrons=
et RX

in Grimentz (Valais) Switzerland
(nice for skiing, isn't it?)
Feb. 25 to March 2 1996

It is intended for people who are not specialized in (all) these methods
and who would like to get a general knowledge of the similarities, the
differences and the potential applications of these techniques. You can get
all the information on Internet

http://cimewww.epfl.ch/avcp/index.html

The lectures will be approx. 60% in French and 40% in English (25 hours at
total, 15 lecturers)
The deadline for registration is Feb. 3

__________________________________________________________________
Philippe Buffat Ecole Polytechnique Federale de
Lausanne (EPFL)
Centre Interdepartemental
de Microscopie Electronique
Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
E-mail: philippe.buffat-at-cime.uhd.epfl.ch // pabuffat-at-cimesg1.epfl.ch
______________________________ Eudora F2.1 ___________________________






From: philippe.buffat-at-cime.uhd.epfl.ch (Philippe-Andre Buffat)
Date: Wed, 24 Jan 1996 08:59:03 +0100
Subject: Winter School/Diffraction

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-Sender: pabuffat-at-cimedec1.epfl.ch
Message-Id: {ad2b944b020210040ae1-at-[128.178.98.14]}
Mime-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable

The Association Vaudoise des Chercheurs en Physique organizes a

**** Winter School****

M=E9thodes modernes de diffusion et de diffraction des neutrons, =E9lectrons=
et RX

in Grimentz (Valais) Switzerland
(nice for skiing, isn't it?)
Feb. 25 to March 2 1996

It is intended for people who are not specialized in (all) these methods
and who would like to get a general knowledge of the similarities, the
differences and the potential applications of these techniques. You can get
all the information on Internet

http://cimewww.epfl.ch/avcp/index.html

The lectures will be approx. 60% in French and 40% in English (25 hours at
total, 15 lecturers)
The deadline for registration is Feb. 3

__________________________________________________________________
Philippe Buffat Ecole Polytechnique Federale de
Lausanne (EPFL)
Centre Interdepartemental
de Microscopie Electronique
Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
E-mail: philippe.buffat-at-cime.uhd.epfl.ch // pabuffat-at-cimesg1.epfl.ch
______________________________ Eudora F2.1 ___________________________






From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Wed, 24 Jan 1996 12:01:05 +0000 (GMT)
Subject: AREMCO fax number

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Dear all,

I am looking for the fax number (or even E-Mail address) of the company
AREMCO products, OSSINING,NY. They sell Aremco crystalbond 509 glue, that
we use for preparing samples.

thank you in advance



Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Luis Sole i Sabaris
E-08028 BARCELONA

Tel +34 3 402 16 95
Fax +34 3 402 13 98





From: Johari, Dr. Om :      73211.647-at-CompuServe.COM
Date: 24 Jan 96 05:47:47 EST
Subject: Scanning Probe Microscopies Meeting at Bethesda during May 11-16, 1996

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Dr. Om Johari * EMC.Ver #2.0 ] --

please circulate/post

Please complete/return form below to remain/get on our lists.

Scanning Microscopy International
Post Office Box 66507, Chicago (A.M.F. O'Hare), IL 60666-0507,
U.S.A.
Telephone: (708) 529-6677 / FAX: (708) 980-6677
E.mail: 73211.647-at-compuserve.com

Scanning Microscopy 1996 meeting
May 11-16, 1996, Bethesda, Maryland (suburb of Washington, DC)

Symposium on: Scanning Probe Microscopies and Related Techniques
for the Biological and Materials Sciences

Note: A number of SPM papers will also be presented durin the
program on "Pattern Formation and Nanoscaled Structures in Thin
Film Formation" at the same time at same venue. THOSE PAPERS ARE
NOT LISTED BELOW. Please request / see a separate flier on that.

Program organizers: Dr. David P. Allison, Oak Ridge Natl. Lab., TN
(E-mail: allisondp-at-ornl.gov); Prof. Chunli Bai, Chinese Acad. Sci.,
Beijing (E.mail: clbai-at-infoc3.icas.ac.cn); Prof. Lawrence A.
Bottomley, Georgia Inst. Tech., Atlanta (E.mail:
lawrence.bottomley-at-chemistry.gatech.edu); Prof. Masamichi Fujihira,
Univ. Yokohama, Japan (phone: 81 22 2152021 / FAX: 81 22 2152020 /
E.mail: mfujihira-at-bio.titech.ac.jp); Dr. Heinrich J.K. Hoerber,
European Molec. Biol. Lab., Heidelberg, Germany (E.mail:
hoerber-at-embl-heidelberg.de); and Prof. Douglas J. Thomson, Univ.
Manitoba, Winnipeg, Canada (E.mail: thomson-at-ee.umanitoba.ca).

Following up on the past SPM meetings, this symposium will provide
a nice occasion to present novel discoveries as well as reviews of
recent developments in theory, instrumentation, and applications of
scanning tunneling microscopy and related techniques, including
atomic force microscopy, magnetic force microscopy, near field
optical microscopy, etc. Applications of STM and other scanning
probe techniques should emphasize the studies of adsorbates as well
as physical and chemical process at solid surfaces. Topics of
interest include: studies of processes on metal, semiconductor, and
other solid surfaces: imaging of molecules, especially
biomolecules; imaging of cells and other biological structures;
tip-induced effects; etc.

Papers can still be offered: please contact one of the organizers
or Dr. Om Johari at Scanning Microscopy International.

List of presentations (as of January 23, 1996) in alphabetical
order

M.J. Allen, Digital Instruments, Santa Barbara, CA et al.:
The Chromatin Structure of Well-Spread Demembranated Human Sperm
Nuclei Revealed by AFM

D.P. Allison et al., Oak Ridge National Lab., TN:
High Resolution Physical Mapping of EcoRI restriction Sites on
Intact Cosmids by AFM Imaging

P.C. Zhang, C. BAI, P.K.H. Ho, Q. Li, Y. Dai, Y.S. Wu, B.F. Sheng,
Chinese Acad. Sci., Beijing:
AFM Study of Interactions Between Tumor Necrosis Factor and Its
Monoclonal Antibodies

J. Bereiter-Hahn et al., Univ. Frankfurt, Germany:
Regulation of Cell Surface Motility, as Revealed by Scanning
Acoustic Microscopy

G. Collins, Topomatrix, Santa Clara, CA:
(1) A Novel High Resolution NSOM; (2) Polymer Science Applications
of a Scanning Thermal Microscope Having a Resistively Heatable
Probe; (3) Applications of a Combined SPM/SEM

E.D. Dahlberg et al., Univ. Minn, Minneapolis:
Review: Magnetic Force Microscopy and Magnetotactic Bacteria

O. Enea, Univ. Poiters, France:
Topographic Studies of TiO2 and SnO2 Ceramics by Environmental SEM,
SEM and AFM

R. ESCHRICH, Max-Planck-Inst. Biochem., Martinsried, Germany; G.L.
Kumar, T.A. Keil, R. Guckenberger:
AFM on the Olfactory Dendrites of Giant Silkmoth Antheraea

M. Fujihara, Tokyo Inst. Tech., Japan:
Review: AFM of Solid Surfaces in Aqueous Solutions

D. Goddard, Br. Nucl Fuels, Preston, UK:
AFM of Bacterial Biofilms with Application to Microbially
Influenced Corrosion

H. Hansma, Univ. Calif., Santa Barbara:
Review: Atomic Force Microscopy of Biomaterials

M. HARA, W. Knoll, RIKEN, Saitama, Japan:
Review: STM and AFM Studies of Self-Assembled Monolayer Growth

D.O. HENDERSON, Y.S. Tung, R. Mu, Fisk Univ., Nashville, TN; W.A.
Curby, M. Mercado, Fed. Aviation Rech. Ctr., Atlantic City, NJ:
Optical and Atomic Force Microscopy of Pentaerythritol Tetranitrate
Nanoclusters on Si(100)

E. Henderson, Iowa State Univ., Ames:
Biomolecular Detection with the AFM (Tentative Title)

H. Hoerber, European Molecular Biology Lab., Heidelberg, Germany:
Measuring Surface Forces with the AFM

D. HUANG, Y. Yamamoto, Yamamoto Quantum Fluctuation Project, Tokyo,
Japan and Stanford Univ., CA:
Hydrogen Atom Extraction and Redeposition on the Monohydride
Si(100)-2x1:H Surface

A. Ikai, Tokyo Inst. Tech., Japan:
Review: Measurements of Mechanical Parameters of Proteins and
Chromosomes with AFM

M.D. JOHNSON, Univ. Oklahoma, Norman, and H.W.M. Salemink:
Review: Cross-Sectional STM on Semiconductor Heterostructures (to
be presented during the Semiconductors program)

G.L. KUMAR, Max-Planck-Inst. Verhaltenphysiol., Seewiesen, Germany;
R. Eschrich, R. Guckenberger, T.A. Keil:
In Search for Putative Pheromone Receptors on the Membrane of
Olfactory Dendrites in Silkmoths (A. polyphemus and A. pernyi)
Using the AFM and SEM

L. Kuutti, VTT Biotech. Food Res., Espoo, Finland:
Identification and Surface Structure of Crystalline Cellulose
Studied by AFM

G. LEE, A.D. MacKerell, L.A. Chrisey, R.J. Colton, US Naval Res.
Lab., Washington, DC:
Measuring Inter- and Intramolecular Forces in Biomolecules

Y. Lyubchenko, Arizona State Univ., Tempe:
AFM Studies of RecA-DNA Complexes

J.F. Marchiando, NIST, Gaithersburg, MD::
Methods for Interpreting Measurements from a Scanning Capacitance
Microscope (to be presented during the Semiconductors program)

M. McDermott, Univ. Alberta, Edmonton, Canada et al.:
Review: Chemical Mapping with Force Microscopy

M. Rivera, M. MILES et al., Univ. Bristol, UK:
Phase Transitions in Liquid Crystals Observed by SPM

W. MIZUTANI, M. Motomatsu, H. Ogiso, H. Tokumoto, Nat. Inst. Adv.
Interdiscpl. Res., Tsukuba, Japan:
STM with Local Non-Linearity Detection of Organic Thin Films and
Ion Irradiated Graphite

D.J. MUELLER, F. Schabert, A. Engel, Univ. Basel, Switzerland:
Review: Structural Changes of Native Membrane Proteins Monitored at
Subnanometer Resolution with the AFM

H. MURAMATSU, Seiko Instruments, Chiba, Japan; T. Ataka, N. Chiba,
K. Nakajima, M. Fujihira
Review: Fluorescence Imaging and Spectroscopy of Biomaterials in
Air and Liquid by SNOM/AFM

P. Nagy, MTA KFKI Res. Inst., Budapest, Hungary:
Review: SPM Image Reconstruction

R. Perez, Univ. Cambridge, UK:
First Principles Simulations of Atomic Resolution in Non-Contact
AFM (to be presented during the Fundamental Physics Program)

R. RAITERI, H.-J. Butt, Max-Planck-Inst. Biophy., Frankfurt,
Germany:
Changes in Surface Stress Measured with an AFM

B. Samori, Univ. Calabria, Bologna, Italy:
DNA Imaging by SFM (exact title to come)

W. Haiss and J.K. SASS, Fritz-Haber-Inst., Berlin, Germany:
STM Surface Stress Measurements in Electrochemistry

Y. Shirane, Univ. Tokushima, Japan:
Surface Observation of Calcium Oxalate Monohydrate Crystals by FE-
SEM and AFM (to be presented during the Stones and Crystals
program)

R.P. Singh, Indian Min. Sci. Tech., New Delhi:
STM and Application Possibilities

V. Snitka et al., Kaunas Univ., Lithuania:
Acoustic Waves Investigation by Atomic Force Microscopy

I. Stangel, McGill Univ., Montreal, Canada:
The Applications and Limitations of AFM to Complex Biomaterials
Surfaces: Effects of Acid Demineralization on Human Dentin

M.S. UNLU, B.B. Goldberg, Boston Univ., MA:
Review: Characterization of Materials and Devices by Near Field
Optical Scanning Microscopy (to be presented during the
Semiconductors program)

J. Vesenka, Calif. State Univ., Fresno:
Review: The Diameter of Duplex and Quadruplex DNA Measured by SPM

VU THIEN BINH, F. Feschet, V. Semet, S.T. Purcell, Univ. Lyon,
France:
Fresnel Projection Microscopy: Theory and Experiment: Electron
Microscopy with Nanometer Resolution at ~ 200 eV

Z.Z. Wang, CNRS Lab. Microstr. Microelec., Bagneux, France:
Pulse Width Dependence of Nanowriting

J.W. Wu, J.H. GU, J.H. Fang, Z.H. Lu, Southeast Univ., Nanjing,
China:
A New Phenomenon at a Small Gap Resistance When Using STM to Modify
Gold Surface

T. WU, Z. Ai, Southeast Univ., Nanjing, China:
Autostereogram: A New Method for Scanning Probe Microscopy

Z.D. Xiao et al., Southeast Univ., Nanjing, China:
A New STM System Using Radioactive Tip

F. Zenhausern, IBM Watson Research Ctr., Yorktown Hts., NY:
New Developments in Near Field Optical Microscopy and Spectroscopy

---

Other related programs at the same venue (*: Fliers available):

*Fundamental Physics in Microscopy and Microanalysis,

*Pattern Formation and Nanoscaled Structures in Thin Film Formation

*Scanning Microscopy and Semiconductors: Metrology and Diagnostics;

Several Biological programs including: Microanalysis and Imaging,
*Immunolabelling, *Radiation Effects, Apoptosis, *Dentistry,
Corrosion Casting, *Inner Ear, *Bone Biology, *Stones and Crystals,
etc.);

Several Biomaterials Related Programs organized under "*Cells and
Materials": (1) Skeletal Tissue / Biomaterials; (2) Foreign Body
Reactions; (3) Biointerfacial Reactions at Biomaterial Surfaces;
(4) Innovative Drug Delivery Systems; (5) Blood-Related
Biomaterials; (6) and (7) Dental Biomaterials.

and Food Structure

----------------

For more information, please complete and return the form below to

__ I wish to present at the Scanning Microscopy 1996 meeting
(tentative title and summary on a separate sheet), please send a
Letter of Intent form.

__ I cannot present, but am likely to attend the 1996 meeting,
please keep me informed and send me: ___ 1996 Registration / Hotel
form.

__ I can neither present nor attend; please add/keep my name on
your mailing list. ___ Send me a mailing list form.

Please send: 1996 program fliers (*list programs here):



___ Instructions for Authors / ___ Major subject index / Table
of Contents for: ___ Scanning Microscopy / ___ Cells and
Materials / ___ Food Structure;

Flier on Scanning Microscopy:

___ Supplement 6, 1992 ("Signal and Image Processing in
Microscopy and Microanalysis");

___ Supplement 7, 1993 ("Physics of Generation and Detection of
Signals Used for Microcharacterization");

___ Supplement 8, 1994 ("Science of Biological Microanalysis")

___ Flier on the special issue: Interface Formation and Dynamics in
Layered Structures (Scanning Microscopy, Vol. 8, no. 4, 1994).

___ Flier on 1996 Pfefferkorn Conference on Electron Image and
Signal Processing, May 18-22, 1996 at Silver Bay, New York


Name

Title

Organization / Company


Address

City/State

Postal Code

Country

Phone

FAX

E.mail

Date




From: Johari, Dr. Om :      73211.647-at-CompuServe.COM
Date: 23 Jan 96 17:30:29 EST
Subject: Electron Image and Signal Processing Conference at Silver Bay, NY, May 18-22

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Dr. Om Johari * EMC.Ver #2.0 ] --

Please Circulate

Scanning Microscopy International
P.O. Box 66507, Chicago (AMF O'Hare), IL 60666, USA
Telephone: (708) 529-6677 / FAX: (708) 980-6677
E.mail:73211.647-at-compuserve.com

Fifteenth Pfefferkorn Conference on
Electron Image and Signal Processing

May 18-22, 1996 at Silver Bay Association, Silver Bay, NY

The Conference will provide a forum for discussion of the current
status, recent advances and prospects of many aspects of image and
signal processing in electron and near-field microscopy and
microanalysis. The principal themes are three-dimensional
reconstruction, image restoration, enhancement and analysis
(including morphological and algebraic approaches in particular),
electron holography, the phase problem, processing image sets (from
STEM detectors and from defocus and tilt series, for example),
image simulation, and hardware and software for image acquisition
and processing and for microscope control. Please see below for a
list of speakers.

The organizers are: Dr. Peter W. Hawkes, CNRS Lab. Opt. Elect.,
B.P. 4347, 31055 Toulouse Cedex, France (Phone: 33-62-257884 / FAX
33-62-257999 / E.mail: hawkes-at-cict.fr); Dr. W. Owen Saxton, Univ.
Cambridge, U.K. (FAX: 44-1223-334 567 / E.mail:
wos1-at-cus.cam.ac.uk); and Dr. Joachim Frank, NYS Dept. Health,
Wadsworth Center, Albany, NY 12201-0509, USA (Phone: 518 474 7002
/ FAX: 518 474 8590 / E.mail: joachim-at-wadsworth.org). Interested
contributors should contact either of the organizers with a title
and a one page summary; please also include other relevant
information (e.g., time desired, reprints of relevant publications,
etc.).

Full-length papers will be published in the Conference Proceedings
to be issued as Scanning Microscopy Supplement 11, 1997.

Conference Information: Registration begins at 6 PM on Friday, May
17. The first lecture will be at 8:30 AM on Saturday, May 18; the
Conference will end at lunch on Wednesday, May 22. Sessions will
be daily in mornings (from 8:30 to 1), afternoons (about 4:30-7),
and evenings (about 8:45 to 10). Ample discussion time will be
allowed; active participation by attendees is invited. Because of
the limited number of attendees, the Pfefferkorn Conferences
provide an excellent opportunity for in-depth discussions and
personal contacts. Conference attendance is by application or
invitation only. Qualified registrants will be accepted on a
first-come-first-served basis. To apply: please submit name,
mailing and E.mail addresses, phone (work and home) and FAX
numbers, and a 50-100 statement about your qualification relative
to the theme (and topics) of the conference, accompanied by the
full registration fee of US $200 (includes attendance to sessions,
coffee-breaks, and a copy of the Conference Proceedings).

Conference Location: The sessions will take place at one of the
conference rooms at Silver Bay Association (SBA), Silver Bay, NY
12874 (phone: 518 543 8833 / FAX: 518 543 6733 / E.mail:
sbaconf-at-aol.com), located 80 miles north of Albany, NY. This 600
acres beautiful, peaceful, registered historical place, located on
Lake George and in the Adirondack mountains, offers dozens of water
and land sports, gym programs, hiking, etc. and is filled with
gardens, rolling lawns and gentle streams. It is ideal for a
family vacation or retreat. The room rates (all rates include
three full meals daily!) range from $40 per person (in two and four
bed-room cottages ideal for four and eight persons, respectively),
to $55 and $65 per person (based on two persons to a room) in rooms
without and with private baths, respectively. Accompanying
children aged 4 to 12 pay half of these rates. Single rooms are
available at a cost of $69 (without private bath) or $80 (with
private bath). It is expected that all attendees will stay at this
location. To make accommodation reservations, please contact SBA
directly and inform us of your travel details (mode and day/time).
Details about getting to Silver Bay will be sent to all attendees:
nearest airports are Albany (New York) and Burlington (Vermont);
foreigners may find it easier to fly to Montreal, Canada (about 150
miles north) or New York (about 200 miles south). There is one
daily train each way from New York and Montreal which stops in
Ticonderoga, NY (about 20 miles from Silver Bay); some bus service
is available too. Appropriate van transportation from Albany (at
costs ranging from $50 per van for less than 4 people, or $10 per
person for more than 5 persons) will also be arranged by SBA.
Attemps will also be made to form car pools with those driving.

Past Conferences: Started in 1982, in honor of the late Prof.
Gerhard E. Pfefferkorn, these Conferences present an in-depth
discussion of fundamental topics in scanning microscopy and related
techniques. Two conferences on topics directly related to the
theme of the 1996 Conference were held in 1987 and 1991;
registrants to the 1996 Conference can obtain those proceedings
[containing original peer-reviewed, full-length papers published as
Scanning Microscopy Supplements 2 (1998) and 6 (1992)] at special
package prices (including uninsured cheapest rate mail delivery) of
$95 (for US delivery) and $105 (for outside US delivery).

Scanning Microscopy International (SMI), a not-for-profit
organization, sponsors the annual Scanning Microscopy, Cells and
Materials, and Food Structure meetings: 1996 meeting will be from
May 11 to 16 at Hyatt Regency Hotel, Bethesda, Maryland (suburb of
Washington, D.C.]; publishes the international journals: Scanning
Microscopy (previously Scanning Electron Microscopy till 1986),
Food Structure, Cells and Materials (from 1991), and Scanning
Microscopy Supplements (Proceedings of the Pfefferkorn
Conferences); and issues publications devoted to selected topics
derived from its journals. Details of the programs planned for the
Scanning Microscopy 1996 meeting, a complete current list of
publications, samples Tables of Contents of publications, etc. are
available on request. The topics of this 1996 Conference are
relevant to the themes of our journals; papers for publication are
invited (Instructions for Authors are included in our journals or
available on request).

For more information, please contact Dr. Om Johari, at SMI.

---

List of speakers as of January 22, 1996 (note: * = invited but not
formally accepted as yet; when there are multiple authors, speakers
name is in CAPITALS).

G. ADE, Physikal.-Techns. Bundes., Braunschweig, Germany:
(1) A Digital Method for Noise Reduction in Holographic
Reconstructions and Electron Microscopical Images
(2) Coma-Free Alignment of Electron Microscopes and Digital
Determination of Aberration Coefficients (with R. Lauer)

G. Anstis, Univ. Technol., Sydney, Australia; C.R. Birkeland, S.C.
Anderson, D.J.H. Cockayne:
Computation and Quantitative Analysis of HAADF Lattice Images of
GaAs

N. Boisset, J.C. Taveau, V. You, F. de Haas, J. Lamy, URA CNRS,
Tours, France:
Refinement of Single Particle 3D Reconstruction (Art Method) Based
on Topological Selection of EM Views

N. Bonnet, Univ. Reims, France:
Multi-Dimensional Spectrum and Image Processing

C.B. Boothroyd, Univ. Cambridge, U.K.:
Recent Results on Energy Filtered Image Quantification

C. BURMESTER, Max-Planck-Inst. Mol. Physiol., Dortmund, Germany;
K.C. Holmes, R.R. Schroeder (Max-Planck-Inst. Med. Forschung,
Heidelburg, Germany):
Feasibility of the Multiple Isomorphous Replacement Method in
Protein-Electron-Diffraction

H. CHENG, T. Baker, Purdue Univ., W. Lafayette, IN:
The Correlation Approach to Virus Phasing

*M. Coster, Univ. Caen, France:
Morphology and SEM Image Analysis: Recent Progress

C. DINGES, H. Rose, Tech. Hochsch., Darmstadt, Germany:
Simulation of TEM Images and Diffraction Patterns Considering
Phonon and Electronic Excitations

J. Frank, NYS Dept. Health, Albany, NY:
Three-Dimensional Reconstruction (exact title to come)

S. Swaminathan, I.P. Jones (Univ. Birmingham, UK), N.J. Zaluzec
(Argonne Natl. Lab.), D.M. Maher (No. Carolina State Univ.,
Raleigh), H.L. FRASER, Ohio State Univ., Columbus:
Determination of Accurate Low Order Structure Factors for Si and
TiAl Using Energy Filtered CBED

D.R. Beniac, G.J. Czarnota(1), F.P. Ottensmeyer(1), G. HARAUZ,
Univ. Guelph, Canada; (1 = Ontario Cancer Institute, Toronto):
Challenges of Three Dimensional Reconstruction of Nucleoprotein
Complexes from Electron Spectroscopic Images

P.W. Hawkes, CNRS Lab. Opt. Electron., Toulouse, France:
Image Algebra and Rank-Order Filters

M.J. Hytch., Ctr. d'Etud. Chim. Metall., CNRS, Vitry, France:
Holographic Reconstruction of Geometric Phase

P. Kruit, B.M. Mertens, Delft Univ. Technol., Netherlands:
Image Acquisition by Scanning in Fourier Space

M.K. KUNDMANN, Gatan EELS Software, Downers Grove, IL; S.L.
Friedman, A.J. Gubbens, O.L. Krivanek:
Characterization and Correction of TEM Energy Filter Aberrations

P.L. Bellon, S. LANZAVECCHIA, Univ. Milan, Italy:
The Moving Window Shannon Reconstruction in Real and Fourier
Domain. Applications in Tomography

H. Lichte, Univ. Dresden, Germany:
Pitfalls on the Road to 0.1 nm with Electron Holography

M. MANKOS, IBM Watson Res. Ctr., Yorktown Hts., NY; M.R.
Scheinfein, J.M. Cowley (Arizona State Univ.):
Holography and the STEM

M.R. McCartney, Arizona State Univ., Tempe:
Recent Applications of Electron Holography

D.G. MORGAN, D.J. DeRosier, Brandeis Univ., Waltham, MA:
Analysis of Disordered Helices Using Correlation Methods

P.D. NELLIST, S.J. Pennycook, Oak Ridge Natl. Lab., TN:
Probe and Object Function Reconstruction in Incoherent STEM Imaging

M.A. O'Keefe, Lawrence Berkeley Lab., Univ. Calif., Berkeley:
On-Line Remote-Control Electron Microscopy with Emphasis on the
Image and Signal Processing

P.A. PENCZEK, J. Zhu, R. Schroeder, J. Frank, NYS Dept. Health,
Albany, NY:
Three-Dimensional Reconstruction with CTF Compensation from Focus
Series

T. Plamann, Univ. Bristol, U.K.:
Three-Dimensional Propagation Effects in Fourier-Resolved
Ptychography

G. Matteucci, G.F. Missiroli, G. POZZI, Univ. Bologna, Italy:
Electron Holography and Electrostatic Fields

M. RADERMACHER, C. Lawrence, NYS Dept. Health, Albany, NY:
Radon Transform Techniques for Alignment and Three-Dimensional
Reconstruction from Random Projections

J.M. Rodenburg, Univ. Cambridge, U.K.:
Practical Double Resolution Projection Imaging in STEM

M. SAUNDERS, P.A. Midgley, R. Vincent, Univ. Bristol, UK:
Quantitative Energy-Filtered CBED: Matching Theory to Experiment

W.O. Saxton, Univ. Cambridge, U.K.:
Microscope Control (Exact title to come)

*M. Schatz, Fritz Haber Inst., Berlin, Germany:
Angular Reconstruction in Three-Dimensional Microscopy

Y. TAKAI, T. Ando, R. Shimizu, Univ. Osaka, Japan:
Spherical Aberration Free Observation by Defocus Image Modulation
Processing Electron Microscopy

T. TANJI, Nagoya Univ., Japan; T. Hirayama (JFCC, Nagoya):
Differential Interferometry by TEM Holography

A. Tonomura, Hitachi Advanced Res. Lab., Saitama, Japan:
Dynamic Observation of Superconducting Vortices by Electron Phase
Microscopy

N.K. TOVEY, J. Wang, Univ. E. Anglia, Norwich, U.K.:
Control Hardware and Software for SEM Image Processing

*K. Urban, Forschungszentrum, Juelich, Germany:
Stochastic Reconstruction Algorithms

D. VAN DYCK, M. Op de Beeck, Univ. Antwerp, Belgium:
From Image to Atomic Structure: How Far Are We?

M. VAN HEEL, E. Orlova, P. Dube, H. Stark, F. Zemlin, M. Schatz,
Fritz Haber Inst., Berlin, Germany:
Angular Reconstitution: High-Resolution Three-Dimensional Structure
From Single Particles

E. VOELKL, L.F. Allard, Oak Ridge Natl. Lab., TN, B. Frost (Univ.
Tennessee):
Electron Holography: Recent Developments and Applications

H.S. von Harrach, VG Scientific, E. Grinstead, U.K.:
Maximum Entropy Reconstruction of STEM Images

Z.L. Wang, Georgia Inst. Tech., Atlanta:
Thermal Diffuse Scattering in Energy Filtered Electron Diffraction
and Imaging

This conferences follows the Scanning Microscopy 1996 meeting in
Bethesda, Maryland from May 11-16 (contact Om Johari at SMI for
more information).

Papers can still be offered, please contact one of the organizers
(see above).




From: Naresh Shah :      naresh-at-service1.uky.edu
Date: Wed, 24 Jan 1996 08:44:00 -0500
Subject: Re: AREMCO fax number

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Message-ID: {310637A0.35BD-at-pop.uky.edu}

Yves Maniette wrote:
}
} Dear all,
}
} I am looking for the fax number (or even E-Mail address) of the company
} AREMCO products, OSSINING,NY. They sell Aremco crystalbond 509 glue, that
} we use for preparing samples.
}
} thank you in advance
}
} Yves MANIETTE
} Universitat de Barcelona
} Serveis Cientifico Tecnics
} Unitat ESCA TEM
} Carrer Luis Sole i Sabaris
} E-08028 BARCELONA
}
} Tel +34 3 402 16 95
} Fax +34 3 402 13 98


Aremco Products, Inc.
P.O. Box 429
Ossining, NY 10562-0429
(914)762-0685
(914)762-1663 (FAX)

I do not have e-mail address.

--

Naresh Shah
CFFLS, 341 Bowman Hall
University of Kentucky
Lexington, KY 40506-0059
e-mail: naresh-at-pop.uky.edu
(606) 257-5119
(606) 257-4027 (Dept. office)
(606) 257-7215 (FAX)




From: Joe D Geller :      geller-at-world.std.com
Date: Wed, 24 Jan 1996 09:45:33 +0001 (EST)
Subject: Re: AREMCO fax number

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Crystalbond is available from AREMCO.
Their phone is 914 762-0685 fax: 914 762-1663.

Joe Geller
Geller Microanalytical Lab
426e Boston St.
Topsfield, MA 01983-1212

On Wed, 24 Jan 1996, Yves Maniette wrote:

}
} Dear all,
}
} I am looking for the fax number (or even E-Mail address) of the company
} AREMCO products, OSSINING,NY. They sell Aremco crystalbond 509 glue, that
} we use for preparing samples.
}
} thank you in advance
}
}
}
} Yves MANIETTE
} Universitat de Barcelona
} Serveis Cientifico Tecnics
} Unitat ESCA TEM
} Carrer Luis Sole i Sabaris
} E-08028 BARCELONA
}
} Tel +34 3 402 16 95
} Fax +34 3 402 13 98
}




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 24 Jan 1996 12:11:31 -0400
Subject: RE-EDS Algorithms

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Subject: Time: 12:04 PM
OFFICE MEMO RE:EDS Algorithms Date: 1/24/96

Those of you interested in callculations such as those involved in processing
EDS spectra should by all means get a copy of the book "Data Reduction and
Error Analysis for the Physical Sciences" by P. R. Bevington, McGraw-Hill,
1969 It contains about a dozen self-consistent FORTRAN programs for
carrying out many of the basic mathematical operations involved in such
calculations. W. C. Bigelow (bigelow-at-umich.edu)





From: keller-at-boulder.nist.gov (Bob Keller)
Date: Wed, 24 Jan 1996 09:38:13 -0700
Subject: Re: EBSP system

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X-Sender: keller-at-arc1.mrd.bldrdoc.gov
Message-Id: {v01510103ad2c10bece11-at-[132.163.192.156]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} I'm trying to get in touch with TSL TexSEM Laboratories Incorporated a
} company that makes EBSP software for Windows. I have a phone number
} 801-467-9930, but nobody answers. Anyone know how to get in touch with
} them?

TSL can be reached at 801-344-8990.

Bob Keller
NIST






From: MEIDJOSTRI-at-engvms.unl.edu (Jo Strinz-Colburn)
Date: Wed, 24 Jan 1996 10:32:13 -0600 (CST)
Subject: Hitachi S-310A field emission SEM

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I am looking for any manuals and/or technical data, i.e. circuit diagrams,
on a Hitachi S-310A field emission SEM. We have the column and vacuum
control/power supplies unit but no scan/display unit and I want to find out
how to connect a computer-based scanning/image acquisition system in its
place for a project.



Thanks for any help you can give me.




Jo Strinz-Colburn
meidjostri-at-engvms.unl.edu

c/o Mechanical Engineering
255 WSEC
Lincoln, NE 68588-0656
(402) 472-7920
(402) 472-1465 FAX






From: Leo Marin :      leo-at-spine.med.utoronto.ca
Date: Wed, 24 Jan 1996 14:38:46 -0500 (EST)
Subject: Formvar detachment

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I have been having a problem during "drop" staining of grids for TEM:
I place my slot grid on a drop of stain. Sometimes the formvar which
holds the sections in place detach from the grid - the formvar (and sections)
float on the drop of stain and the grid sinks to the bottom.Has anyone
out there been having a similar problem? Do you know of any solution. I
have taken a number of "remedial" measures but I am not sure whether
they have worked since the problem re-occurs from time to time. For example
I have :
- stained grids after allowing them to "stand" for at least 24 hours
after sectioning.
- tried multiple grid staining
- used a glue pen
- pre-dipped grids in formvar before picking up sections
- cleaned grids thoroughly in acetone-HCl- DW
-used acetone-stored grids
-left grids with sections (on formvar plates) in a desiccator for
extended periods
I would appreciate any suggestions.

Leo




From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 24 Jan 1996 09:59:58 -0500 (EST)
Subject: Re: TEM contrast

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} One of my courses will discuss in length the contrast
} theory used for the TEM.
} Since I've never ever worked with these beasts I wonder if there is a
} S/W simulator or some other means that wouldmake the digestion of the
} theory a little easier ???
}
} I have to start from electron diffraction, white field, dark field,
} Kikuchi pattern etc... all new to me ! to get to the contrast theory.

Dear CFILION,
The Academic Press volumes, Principles of Electron Optics, by
Hawkes & Kasper, go into this in great detail in Vol. 3. Good luck.
Yours,
Bill Tivol




From: jbpawley-at-facstaff.wisc.edu (Jim Pawley)
Date: Wed, 24 Jan 1996 09:21:58 -0600
Subject: "3D MICROSCOPY OF LIVING CELLS" Course, Second announcement

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********** PLEASE RESPOND BY EMAIL TO:jbpawley-at-facstaff.wisc.edu: **********

LIMITED SPACE STILL AVAILABLE.
________________________________________________________________________________

Second announcement:

An intensive, 8-day course on

"3D MICROSCOPY OF LIVING CELLS"

will be given at the

University of British Columbia, Vancouver, BC, Canada

July 27 - August 4, 1996

THE PURPOSE OF THE COURSE

Modern methods of 3D light microscopy promise a revolutionary improvement
in our ability to view living cells. To help convert this promise to
reality for a wider selection of biological scientists, an intensive eight
day residential course concentrating on all aspects of the 3D Microscopy of
Living Cells will be introduced at the University of British Columbia, in
the summer of 1996.
Covering everything from basic microscopy to the technical considerations
that define the highest levels of performance of the confocal microscope,
this course will include:

* Quantitative confocal microscopy
* Pixelation: The Nyquist Criterion
* Lasers and laser tweezers
* Objectives and aberrations
* Scanning-systems
* Wide field/deconvolution techniques
* Detectors: operation and performance
* Optimal pinhole size & photon efficiency
* Dye design, characteristics and use
* How to keep your cells alive
* Two-photon excitation
* Video-rate confocal imaging
* Measuring ion concentrations
* Display and measurement of 3D data
* Digital hard copy and storage
* Fluorescent & gold labeling of living cells
* Backscattered light imaging.

Lecture demonstrations will be interspersed with hands-on laboratory
exercises that will utilize all of the currently available commercial
instruments for 3D microscopic imaging.
Students will work in groups of three throughout the discussion and
laboratory sessions, and will complete a live-cell 3D study on a specimen
of their choice.
At least seven, separate 3D microscopical workstations, attended by a
technical staff of 15, will be available for student use. Overall, the
teacher/student ratio will be more than 2:1.

International Academic Faculty:

* Jon Art University of Illinois
* Milton Charlton University of Toronto
* Rachel Errington Oxford University
* Jim Pawley University of Wisconcon-Madison
* Wallace Marshall U. of California, San Francisco
* Ernst Stelzer EMBL, Heidelberg
* Roger Tsien University of California, San Diego
* Pavel Vesely Academy of Sciences, Prague
* Watt Webb Cornell University
* Michael Weis University of British Columbia
* Nick White Oxford University

International Commercial Faculty:

* Ernst Keller Carl Zeiss, NY
* Paul Millard Molecular Probes, OR
* Sigrid Myrdal Bristol-Myers Squibb, WA
* K. Sam Wells Bio-Rad,,CA

WHO SHOULD ATTEND?

The course is designed for biological research scientists and advanced
graduate students who use, or plan to apply 3D microscopy to studies
involving living cells. No previous experience in advanced light
microscopy is required but applicants will be asked to outline how they
plan to use 3D microscopy and describe a short research project that they
plan to carry out during the course. Most projects will If space permits,
students with other interests in 3D light microscopy will be welcomed.

PLAN OF INSTRUCTION

Classes will meet from 8:30 -12:30 and 1:30 - 5:30 and will alternate
between lecture-demonstrations and laboratory sessions. From Monday to
Friday, facilities and supervision will be available until 11:00 pm, for
students to work on their projects. On average, only two topics will be
covered in each morning or afternoon session.
There will be enough 3D microscopy setups to permit students in groups
of three, to "learn-by-doing" following each demonstration. Lab handouts
will include detailed questions to stimulate group discussions.
Prior to the course, students will be organized into groups and encouraged
to communicate by email/phone, about the "Living-cell" group projects that
they will pursue in the evenings and that will be presented to the class on
the last day of the course.
Students must contact the Course Organizer to make necessary
arrangements for the transport and maintenance of cell lines etc. needed
for their projects.

ACCOMMODATIONS

Campus accommodations border on the luxurious and arrangements can easily
be made for accompanying family members. Rooms and suites will be
situated in the Walter Gage Residence located centrally, a few blocks from
either the lecture-lab facilities or the Student Union Building which
contains a large cafeteria, lounge, bank, etc. Many of the rooms in the
Gage Residence have breathtaking views of the mountains of North and West
Vancouver, and the Pacific Ocean. A variety of accommodation types are
available:

- Single room with shared washroom $24

- Single room with private bath $41

- Double suite (kitchenette, private bath,
TV, phone, double bedroom plus
separate sitting room) $63

- Triple suite (twin bedroom and queen
Murphy bed in sitting room, balcony,
kitchen, bath, TV, phone) $74
(All fees are $ US per night. Add 15% VAT)


Students are encouraged to bring family members to enjoy the pristine
beauty of the Vancouver area and the miles of sandy beaches that surround
the campus.


TUITION

Tuition is $1,500 US. On receipt of the deposit, all students will
receive preliminary assignments and a copy of the textbook, "Handbook of
Biological Confocal Microscopy," (Plenum, 1995).
The tuition fee includes one ticket for the opening reception and the
banquet, the textbook and all handouts. Accommodations and meals are not
included in the tuition fee.

APPLICATIONS

Applicants will complete a questionnaire to assess knowledge level and
field of interest. Enrolment will be limited to 20 participants.
Selection will be made on the basis of background and perceived need.
Those without previous LM experience will be provided with basic texts to
read before the course begins. Application packages may be obtained from


Prof. James Pawley, Rm. 1235,
1500 Johnson Dr., Madison, WI, USA 53706.
Phone: 1-608-263-3147, Fax 1-608-265-5315,
Email: jbpawley-at-facstaff.wisc.edu

Application deadlines:

Applications forms requesting information on field of interest and level
of experience must be received for screening by March 1, 1996.
Successful applicants will be notified by April 1, and a deposit of 50%
must be received by April 15, 1996. In general, refunds of the deposit
will not be possible.


Applications must be received by Mar. 1/96
50% deposit due Apr. 15/96
Registration 3:00 - 5:00 pm Sat., July 27/96
Last class will end with lunch Sun., Aug. 4/96



*****************************************

****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU

****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Wed, 24 Jan 1996 16:29:26 -0600
Subject: Re: Charging for Services

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Message-Id: {199601242132.PAA23101-at-BCM.TMC.EDU}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

At 08:55 AM 1/23/96 -1000, you wrote:
} As I am in one of the facilities who used to have a "pre-paid, lump-sum"
} system in place that appeared to make everybody happy, only to have a
} federal audit come in and SEVERLY penalize us for doing so, I would also
} be interested in what other recharge facilities are doing. Please keep
} this dialog going, or post a compilation of replies!
}
} Mahalo,
} Tina
}
************************
Tina -

I am also in a fee-for-service situation. I get no support from grants,
except that investigators use funds from their grants to pay me for the
electron microscopy I do for their research projects. We have a fee
structure that basically prices our services depending upon what we are
called on to do, and whether there is a signed report that goes into a
patient's file in clinical cases. Some time ago when I was setting this up
and was curious what other service EM labs, especially medical labs like my
own, charged for their work, I was told that asking other labs what they
charged was illegal and amounted to price fixing. I am not sure what agency
enforces that, probably Medicare; and I am not sure if it is still true. But
if you have medical cases, you might inquire about this.


Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 1/23/96 12:53 PM
Subject: cataracts

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About a year ago, after using EM's for 27 years and having
developed cataracts in both eyes at an unusually early age
(49), I asked the question through this medium about eye damage
resulting from long-term use of electron microscopes. As in the
current discussion some interesting comments were made. There is
definitely evidence that radiation from a variety of sources
causes cataracts, typically in the posterior capsule of the lens
- not the nucleus where senile cataracts usually develop.
However, modern electron microscopes should be adequately
screened to prevent this. In my case the cataracts were
apparently typical of radiation-induced cataracts although when
and where the irradiation took place is anyone's guess. Someone
has mentioned UV radiation from vacuum evaporators. This could
be a possibility because I know of little in the way of
precautions which are routinely taken to prevent this.



Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)





From: R Holland Cheng :      RHC-at-justem.bio.purdue.edu
Date: Wed, 24 Jan 1996 12:28:02 -0500 (EST)
Subject: FWD: Meeting at Silver bay

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} Dear Dr. Cheng,
}
} Thank you for your reply.
}
} I have not had good success with the E.mails publicity. I provide the
} latest flier below. Can you please help me by having it posted? Any other
} help you can give to publicize the conference will be most appreciated.
} ----



-- [ From: Dr. Om Johari * EMC.Ver #2.0 ] --

Please Circulate

Scanning Microscopy International
P.O. Box 66507, Chicago (AMF O'Hare), IL 60666, USA
Telephone: (708) 529-6677 / FAX: (708) 980-6677
E.mail:73211.647-at-compuserve.com

Fifteenth Pfefferkorn Conference on
Electron Image and Signal Processing

May 18-22, 1996 at Silver Bay Association, Silver Bay, NY

The Conference will provide a forum for discussion of the current
status, recent advances and prospects of many aspects of image and
signal processing in electron and near-field microscopy and
microanalysis. The principal themes are three-dimensional
reconstruction, image restoration, enhancement and analysis
(including morphological and algebraic approaches in particular),
electron holography, the phase problem, processing image sets (from
STEM detectors and from defocus and tilt series, for example),
image simulation, and hardware and software for image acquisition
and processing and for microscope control. Please see below for a
list of speakers.

The organizers are: Dr. Peter W. Hawkes, CNRS Lab. Opt. Elect.,
B.P. 4347, 31055 Toulouse Cedex, France (Phone: 33-62-257884 / FAX
33-62-257999 / E.mail: hawkes-at-cict.fr); Dr. W. Owen Saxton, Univ.
Cambridge, U.K. (FAX: 44-1223-334 567 / E.mail:
wos1-at-cus.cam.ac.uk); and Dr. Joachim Frank, NYS Dept. Health,
Wadsworth Center, Albany, NY 12201-0509, USA (Phone: 518 474 7002
/ FAX: 518 474 8590 / E.mail: joachim-at-wadsworth.org). Interested
contributors should contact either of the organizers with a title
and a one page summary; please also include other relevant
information (e.g., time desired, reprints of relevant publications,
etc.).

Full-length papers will be published in the Conference Proceedings
to be issued as Scanning Microscopy Supplement 11, 1997.

Conference Information: Registration begins at 6 PM on Friday, May
17. The first lecture will be at 8:30 AM on Saturday, May 18; the
Conference will end at lunch on Wednesday, May 22. Sessions will
be daily in mornings (from 8:30 to 1), afternoons (about 4:30-7),
and evenings (about 8:45 to 10). Ample discussion time will be
allowed; active participation by attendees is invited. Because of
the limited number of attendees, the Pfefferkorn Conferences
provide an excellent opportunity for in-depth discussions and
personal contacts. Conference attendance is by application or
invitation only. Qualified registrants will be accepted on a
first-come-first-served basis. To apply: please submit name,
mailing and E.mail addresses, phone (work and home) and FAX
numbers, and a 50-100 statement about your qualification relative
to the theme (and topics) of the conference, accompanied by the
full registration fee of US $200 (includes attendance to sessions,
coffee-breaks, and a copy of the Conference Proceedings).

Conference Location: The sessions will take place at one of the
conference rooms at Silver Bay Association (SBA), Silver Bay, NY
12874 (phone: 518 543 8833 / FAX: 518 543 6733 / E.mail:
sbaconf-at-aol.com), located 80 miles north of Albany, NY. This 600
acres beautiful, peaceful, registered historical place, located on
Lake George and in the Adirondack mountains, offers dozens of water
and land sports, gym programs, hiking, etc. and is filled with
gardens, rolling lawns and gentle streams. It is ideal for a
family vacation or retreat. The room rates (all rates include
three full meals daily!) range from $40 per person (in two and four
bed-room cottages ideal for four and eight persons, respectively),
to $55 and $65 per person (based on two persons to a room) in rooms
without and with private baths, respectively. Accompanying
children aged 4 to 12 pay half of these rates. Single rooms are
available at a cost of $69 (without private bath) or $80 (with
private bath). It is expected that all attendees will stay at this
location. To make accommodation reservations, please contact SBA
directly and inform us of your travel details (mode and day/time).
Details about getting to Silver Bay will be sent to all attendees:
nearest airports are Albany (New York) and Burlington (Vermont);
foreigners may find it easier to fly to Montreal, Canada (about 150
miles north) or New York (about 200 miles south). There is one
daily train each way from New York and Montreal which stops in
Ticonderoga, NY (about 20 miles from Silver Bay); some bus service
is available too. Appropriate van transportation from Albany (at
costs ranging from $50 per van for less than 4 people, or $10 per
person for more than 5 persons) will also be arranged by SBA.
Attemps will also be made to form car pools with those driving.

Past Conferences: Started in 1982, in honor of the late Prof.
Gerhard E. Pfefferkorn, these Conferences present an in-depth
discussion of fundamental topics in scanning microscopy and related
techniques. Two conferences on topics directly related to the
theme of the 1996 Conference were held in 1987 and 1991;
registrants to the 1996 Conference can obtain those proceedings
[containing original peer-reviewed, full-length papers published as
Scanning Microscopy Supplements 2 (1998) and 6 (1992)] at special
package prices (including uninsured cheapest rate mail delivery) of
$95 (for US delivery) and $105 (for outside US delivery).

Scanning Microscopy International (SMI), a not-for-profit
organization, sponsors the annual Scanning Microscopy, Cells and
Materials, and Food Structure meetings: 1996 meeting will be from
May 11 to 16 at Hyatt Regency Hotel, Bethesda, Maryland (suburb of
Washington, D.C.]; publishes the international journals: Scanning
Microscopy (previously Scanning Electron Microscopy till 1986),
Food Structure, Cells and Materials (from 1991), and Scanning
Microscopy Supplements (Proceedings of the Pfefferkorn
Conferences); and issues publications devoted to selected topics
derived from its journals. Details of the programs planned for the
Scanning Microscopy 1996 meeting, a complete current list of
publications, samples Tables of Contents of publications, etc. are
available on request. The topics of this 1996 Conference are
relevant to the themes of our journals; papers for publication are
invited (Instructions for Authors are included in our journals or
available on request).

For more information, please contact Dr. Om Johari, at SMI.

---

List of speakers as of January 22, 1996 (note: * = invited but not
formally accepted as yet; when there are multiple authors, speakers
name is in CAPITALS).

G. ADE, Physikal.-Techns. Bundes., Braunschweig, Germany:
(1) A Digital Method for Noise Reduction in Holographic
Reconstructions and Electron Microscopical Images
(2) Coma-Free Alignment of Electron Microscopes and Digital
Determination of Aberration Coefficients (with R. Lauer)

G. Anstis, Univ. Technol., Sydney, Australia; C.R. Birkeland, S.C.
Anderson, D.J.H. Cockayne:
Computation and Quantitative Analysis of HAADF Lattice Images of
GaAs

N. Boisset, J.C. Taveau, V. You, F. de Haas, J. Lamy, URA CNRS,
Tours, France:
Refinement of Single Particle 3D Reconstruction (Art Method) Based
on Topological Selection of EM Views

N. Bonnet, Univ. Reims, France:
Multi-Dimensional Spectrum and Image Processing

C.B. Boothroyd, Univ. Cambridge, U.K.:
Recent Results on Energy Filtered Image Quantification

C. BURMESTER, Max-Planck-Inst. Mol. Physiol., Dortmund, Germany;
K.C. Holmes, R.R. Schroeder (Max-Planck-Inst. Med. Forschung,
Heidelburg, Germany):
Feasibility of the Multiple Isomorphous Replacement Method in
Protein-Electron-Diffraction

H. CHENG, T. Baker, Purdue Univ., W. Lafayette, IN:
The Correlation Approach to Virus Phasing

*M. Coster, Univ. Caen, France:
Morphology and SEM Image Analysis: Recent Progress

C. DINGES, H. Rose, Tech. Hochsch., Darmstadt, Germany:
Simulation of TEM Images and Diffraction Patterns Considering
Phonon and Electronic Excitations

J. Frank, NYS Dept. Health, Albany, NY:
Three-Dimensional Reconstruction (exact title to come)

S. Swaminathan, I.P. Jones (Univ. Birmingham, UK), N.J. Zaluzec
(Argonne Natl. Lab.), D.M. Maher (No. Carolina State Univ.,
Raleigh), H.L. FRASER, Ohio State Univ., Columbus:
Determination of Accurate Low Order Structure Factors for Si and
TiAl Using Energy Filtered CBED

D.R. Beniac, G.J. Czarnota(1), F.P. Ottensmeyer(1), G. HARAUZ,
Univ. Guelph, Canada; (1 = Ontario Cancer Institute, Toronto):
Challenges of Three Dimensional Reconstruction of Nucleoprotein
Complexes from Electron Spectroscopic Images

P.W. Hawkes, CNRS Lab. Opt. Electron., Toulouse, France:
Image Algebra and Rank-Order Filters

M.J. Hytch., Ctr. d'Etud. Chim. Metall., CNRS, Vitry, France:
Holographic Reconstruction of Geometric Phase

P. Kruit, B.M. Mertens, Delft Univ. Technol., Netherlands:
Image Acquisition by Scanning in Fourier Space

M.K. KUNDMANN, Gatan EELS Software, Downers Grove, IL; S.L.
Friedman, A.J. Gubbens, O.L. Krivanek:
Characterization and Correction of TEM Energy Filter Aberrations

P.L. Bellon, S. LANZAVECCHIA, Univ. Milan, Italy:
The Moving Window Shannon Reconstruction in Real and Fourier
Domain. Applications in Tomography

H. Lichte, Univ. Dresden, Germany:
Pitfalls on the Road to 0.1 nm with Electron Holography

M. MANKOS, IBM Watson Res. Ctr., Yorktown Hts., NY; M.R.
Scheinfein, J.M. Cowley (Arizona State Univ.):
Holography and the STEM

M.R. McCartney, Arizona State Univ., Tempe:
Recent Applications of Electron Holography

D.G. MORGAN, D.J. DeRosier, Brandeis Univ., Waltham, MA:
Analysis of Disordered Helices Using Correlation Methods

P.D. NELLIST, S.J. Pennycook, Oak Ridge Natl. Lab., TN:
Probe and Object Function Reconstruction in Incoherent STEM Imaging

M.A. O'Keefe, Lawrence Berkeley Lab., Univ. Calif., Berkeley:
On-Line Remote-Control Electron Microscopy with Emphasis on the
Image and Signal Processing

P.A. PENCZEK, J. Zhu, R. Schroeder, J. Frank, NYS Dept. Health,
Albany, NY:
Three-Dimensional Reconstruction with CTF Compensation from Focus
Series

T. Plamann, Univ. Bristol, U.K.:
Three-Dimensional Propagation Effects in Fourier-Resolved
Ptychography

G. Matteucci, G.F. Missiroli, G. POZZI, Univ. Bologna, Italy:
Electron Holography and Electrostatic Fields

M. RADERMACHER, C. Lawrence, NYS Dept. Health, Albany, NY:
Radon Transform Techniques for Alignment and Three-Dimensional
Reconstruction from Random Projections

J.M. Rodenburg, Univ. Cambridge, U.K.:
Practical Double Resolution Projection Imaging in STEM

M. SAUNDERS, P.A. Midgley, R. Vincent, Univ. Bristol, UK:
Quantitative Energy-Filtered CBED: Matching Theory to Experiment

W.O. Saxton, Univ. Cambridge, U.K.:
Microscope Control (Exact title to come)

*M. Schatz, Fritz Haber Inst., Berlin, Germany:
Angular Reconstruction in Three-Dimensional Microscopy

Y. TAKAI, T. Ando, R. Shimizu, Univ. Osaka, Japan:
Spherical Aberration Free Observation by Defocus Image Modulation
Processing Electron Microscopy

T. TANJI, Nagoya Univ., Japan; T. Hirayama (JFCC, Nagoya):
Differential Interferometry by TEM Holography

A. Tonomura, Hitachi Advanced Res. Lab., Saitama, Japan:
Dynamic Observation of Superconducting Vortices by Electron Phase
Microscopy

N.K. TOVEY, J. Wang, Univ. E. Anglia, Norwich, U.K.:
Control Hardware and Software for SEM Image Processing

*K. Urban, Forschungszentrum, Juelich, Germany:
Stochastic Reconstruction Algorithms

D. VAN DYCK, M. Op de Beeck, Univ. Antwerp, Belgium:
From Image to Atomic Structure: How Far Are We?

M. VAN HEEL, E. Orlova, P. Dube, H. Stark, F. Zemlin, M. Schatz,
Fritz Haber Inst., Berlin, Germany:
Angular Reconstitution: High-Resolution Three-Dimensional Structure
From Single Particles

E. VOELKL, L.F. Allard, Oak Ridge Natl. Lab., TN, B. Frost (Univ.
Tennessee):
Electron Holography: Recent Developments and Applications

H.S. von Harrach, VG Scientific, E. Grinstead, U.K.:
Maximum Entropy Reconstruction of STEM Images

Z.L. Wang, Georgia Inst. Tech., Atlanta:
Thermal Diffuse Scattering in Energy Filtered Electron Diffraction
and Imaging

This conferences follows the Scanning Microscopy 1996 meeting in
Bethesda, Maryland from May 11-16 (contact Om Johari at SMI for
more information).

Papers can still be offered, please contact one of the organizers
(see above).





From: Martin K|hler :      mk-at-enk.ks.se
Date: Thu, 25 Jan 1996 01:00:48 +0100
Subject: LM: uncaging instrumentation

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Equipment for "UNCAGING OF CAGED SUBSTANCES"?

I wonder what equipment is used or should be used for uncaging (photolysis)
of caged (photoactivable) compounds.

Background:
Caged compounds usually seems to be optimally activated by a short
(millisecond) pulse of {365 nm light. To get this light one may use light
sources like lasers, flash lamps or maybe arclamps with fast shutter. The
light may reach the sample either through the objective (light through
epifluorescence port of the microscope) or by leading an optical fibre
directly to the cell.

Questions about instrumentation:

- What light sources are used (types of lasers, Xenon arc lamps, Mercury arc
lamps, flash lamps)?

- How is the exact light path to the cell/cells/subcells? What types of
optics and components are used?

- What effect or energy is needed?

- What companies provide necessary parts (like Oriel, Newport, Melles Griot,
Hamamatsu)?=20

- What complete commersial systems are available for this, and how are their
performance?


Thanks in beforehand for your answers,
Martin

-----------------------------------------------
Martin K=F6hler
Department of Molecular Medicine
Rolf Luft Center for Diabetes Research L6B:1
Karolinska Hospital
S-171 76 STOCKHOLM
SWEDEN
phone 46-8-7295732
46-8-7295725
fax 46-8-7293658
E-mail mk-at-enk.ks.se
---------------------------------------------





From: Miguel Avalos B. :      miguel-at-ifisicaen.unam.mx
Date: Wed, 24 Jan 1996 19:53:07 -0800
Subject: ION MILL WANTED..

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I'm looking for a used (and inexpensive) ion mill device for the preparation
of TEM specimens..

Miguel Avalos
IFUNAM
Ensenada, B.C. MEXICO




From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Thu, 25 Jan 1996 09:52:52 +0000 (GMT)
Subject: glues:more

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Dear all,

Here is another message about glues for sample preparation. Hope it helps.
YM.


I saw your message om the Crystalbond. Please note that we sell the
Crystalbond in our catalog and at very competitive prices. If you are
interested please see page 147 of catalog XII. If you do not have this
catalog please let me know and we will send you one.
I hope this information helps.

Stacie Kirsch
Electron Microscopy Sciences.
Tel: 215-646-1566
Fax: 215-646-8931





From: Brett W. Cockman :      bcockman-at-uniwa.uwa.edu.au
Date: Thu, 25 Jan 1996 16:42:40 +0700 (WAST)
Subject: Thanks Re: Ralph knife glass

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Just a note of thanks to all those who provided their views and
recommendations regarding the use of ultramicrotome quality glass (UMG) v's
histology quality glass. The upshot is that UMG should be more than adequate
for the purpose of making Ralph knives.
Thanks again for your interest.

Brett.
Brett W. Cockman
Technologist in Charge
School of Dentistry
University of Western Australia
Voice: (619-2205834)
Fax: (619-2213829)
e-mail; bcockman-at-uniwa.uwa.edu.au




From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Thu, 25 Jan 1996 09:47:09 +0000 (GMT)
Subject: Sample preparation glues

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Dear all,

I got an interesting piece of information about glues for TEM sample
preparation, from D. Henriks, South Bay Technology.
So I am sending it to the list, as it might be of interest. Particularly
I know that it is quite difficult to get the AREMCO 509 glue in Europe,
because it has to go through a network of distributors and
sub-distributors, so that the price comes to be quite elevated.

-- MESSAGE --

We can supply it to you in the same 7" long sticks that you get from Aremco or
you can buy a package of 20 3" long x 1/4" x 1/4" Unwrapped sticks. These are
ideal for sample preparation. I absolutely guarantee that you will be 100%
satisfied with the product.

Below, I have listed quantity pricing and alternative packaging for the
QuickStick 135. This material is the same as "Crystalbond 509".

20 x 3.5" Sticks per tray (360 grams per tray) - this is our standard package.
Minimum order is 1 tray.

1 x .875" diameter x 7" long wrapped in cardboard tube (5 sticks = 450 grams).
Minimum order is 10 sticks.

10 x .875" diameter x 7" long unwrapped in plastic tray (1 tray = 900 grams).
Minimum order is 1 tray.

1 pound bulk pack trays
Minimum order is 10 pounds

Sizes and gross weights for above are approximate.


David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
sbt-at-msa.microscopy.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 25 Jan 96 08:34:03 EST
Subject: Re: Formvar detachment

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Detachment of formvar films from grids during staining is a common problem. We
recommend use of the SynapTek grid stick kit, which holds the grids during
staining in the same plane as the solution flow, minimizing the chances of
losing the films (or collecting surface debris). The grids are held to the grid
stick with a special adhesive which is not attacked by solvents, and which will
not remain on the grid once it is removed from the stick.
Please contact me directly for specific ordering information.
Steven Slap, Vice-President
Energy Beam Sciences, Inc
75767,640-at-compuserve.com





From: krogers-at-ecn.purdue.edu (Kirk Rogers)
Date: Thu, 25 Jan 1996 10:41:18 -0500
Subject: RE:glues

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All-

I used the Crystalbond 509 glue forTEM prep initially, but about a 2 years
ago I switched to quick-bond nail glue (for cosmetic fingernails - a
cyanoacrylate formula, like SuperGlue) for several reasons:
1. specimens do not have to be heated, which seemed to be a problem with
the specimen mounts for the dimpler I was using

2. the glue thickness is about half of what I could achieve with
Crystalbond, which increased my success rate at preparing copper-sapphire
interface specimens

3. specimen adhesion is nearly instantaneous - instant gratification!

4. I can walk across the street and purchase more instead of waiting 3
weeks for the purchase order to get through purchasing

5. Crystalbond properties seemed to degrade during our humid summers.

The only real problems with the stuff are that it takes ~2 hours of soaking
in acetone to remove specimens from the dimpler mount, and when not capped
tightly an entire bottle will cure in about 2 weeks.

For larger jobs, I use a homemade version of the Crystalbond glue (found in
an old Handbook of Chemistry and Physics) which can be made by mixing
equimolal quantities of phthalic anhydride and ethylene glycol for 24 hours
at 200 deg. C in a covered beaker, stirring every couple of hours. Pour
into some sort of flexible mold to make shapes and store in a cool dry
place.




-Kirk
_____________________________________________
Kirk Rogers krogers-at-materials.ecn.purdue.edu
OR kirk.a.rogers.1-at-purdue.edu
Purdue University, School of Materials Science and Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289
OFFICE: 317-494-8751 FAX: 317-494-1204
http://materials.ecn.purdue.edu/~krogers






From: houpt-at-worldaccess.nl (houpt)
Date: Thu, 25 Jan 1996 17:30:51 +0100
Subject: toplens wanted

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For planktonresearch I use an old Leitz Ortholux stand.On this stand a
socalled Berek condensor is used with interchangeable toplenses.
I look for an small toplens of NA 1.4 which fits onto that condensor.
Can any microscopist help me , for a reasonable price.

Thank you in advance

Pieter Houpt

Pieter.M.Houpt.
Marine Plankton & microscopy
tel/fax. 003170504466
The Hague.
The Netherlands.






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 25 Jan 1996 11:24:04 -0600
Subject: Re: Charging for Services

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At 08:51 AM 1/25/96 -0600, you wrote:

} Let me get this straight: you are required to charge "customary fees" for
} your services (otherwise you won't get paid), but you are not allowed to
} find out what the usual fees are? Did anyone ever try a class in elementary
} logic on these guys who make the rules?
}
*******************
I just went down the hall to check with our administrative associate in
charge of billing. Yes, I am right: for me (Director of the medical EM lab)
to ask people in another medical lab, not within our organization, what
their charges are for their services is illegal. As Chuck Garber, points
out, it runs afoul of the anti trust laws. I can ask for, and the other lab
can give me, a RANGE within which their charges fall, but they cannot
disclose, nor can I demand to know, what the actual numbers are. No, I do
not know how broad that "range" must be.


Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 25 Jan 1996 11:24:02 -0600
Subject: Re: Charging for Services

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At 07:22 AM 1/25/96 EST, you wrote:

} While you should not take this as legal advice, at no time has the
} congress ever exempted nonprofit or other tax exempt organizations from
} the anti trust laws. If anyone should ever challenge you on that one,
} ask them to show you where nonprofits were ever given any such
} exemption.
*******************
I assumed that to be the case.
*******************


} Secondly, I for one, sitting in the private sector, get quite a kick
} out of these kinds of conversations. It sounds to me like the decision
} on what to charge someone is about as arbitrary as could be, and there
} also seems to be an admission that there is little relationship between
} what someone costs vs. how it is charged.
*******************
For years that's exactly what I based my charges on, ie. my costs plus a bit
to cover replacement and depreciation. The goal was to run a lab that paid
for itself. There was no pressure to make a profit strictly for profit's
sake. Now with the new politics in health care, the "bottom line" is more
important than patient care, and I am told what to charge.
*******************


} Yet, how would you feel if I was selling you a sputter coater 20% more
} than I would charge someone else only because, say, I thought you could
} afford it more than someone else? I am sure you would not feel very
} good about that at all, as indeed you should feel.


*******************
Hey, it happens! Not from reputable suppliers such as SPI....but it happens.

I appreciate your thoughts on these matters, Chuck, because you view things
from a different perspective.


Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: brmjg-at-ttacs1.ttu.edu (Mark Grimson)
Date: Thu, 25 Jan 1996 13:16:06 -0600 (CST)
Subject: Subscription

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Could you please add my name to the subscription file. Thank you.






From: Kathy Walters :      kwalters-at-emiris.iaf.uiowa.edu
Date: Thu, 25 Jan 1996 14:36:05 -0600 (CST)
Subject: Thin sections of polymers

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Esteemed Colleagues:

I have been asked to image the interior structure of a polymer
suspension.

Ideally I would like to resin embed for TEM. I am not a polymer
scientist, but I do know that these polymers produce spheres when
suspended with small amounts of BSA. The polymer is polyglycolic acid
(PGLA). I called the manufacturers, but they have not done any
significant imaging of it, and do not appear to be interested. They did
tell me that the PGLA is heat sensitive and dissolves in isopropal
alcohol. My experiment with epoxys was an unmitigated disaster, and I am
considering a low temperature embedment procedure (Another researcher
tried Lowicryl, but the spheres did not remain intact).

I referenced a book entitled "Polymer Microscopy" by Sawyer and
Grubb, but it doesn't refer to anything similar to my polymer.

Any (relevent) thoughts would be appriciated.

Kathy Walters
CMRF
U of Iowa





From: John.Wheatley-at-ASU.Edu (John C. Wheatley)
Date: Wed, 24 Jan 1996 15:00:12 -0700
Subject: CCD Cameras for Light Microscopy

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If this question has been asked before, I apologize. I need information on
your experiences with light microscopy CCD camera systems. What systems
are available for easily and inexpensively (?) digitizing images from light
microscopes to PC formats? I need manufacturers (addresses) and prices
please. Please send information to my e-mail address--especially if this
discussion has taken place before. Thank you.

John C. Wheatley
Lab Manager
Center for High Resolution Electron Microscopy
BOX 871704
Tempe, AZ 85287-1704
Phone: (602) 965-3831
FAX: (602) 965-9004
John.Wheatley-at-ASU.Edu






From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 25 Jan 1996 17:02:58 -0500 (EST)
Subject: Re: LM: uncaging instrumentation

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} I wonder what equipment is used or should be used for uncaging (photolysis)
} of caged (photoactivable) compounds.
} [snip]
} - What light sources are used (types of lasers, Xenon arc lamps, Mercury arc
} lamps, flash lamps)?
}
} - How is the exact light path to the cell/cells/subcells? What types of
} optics and components are used?
} [snip]

Dear Martin and especially SPM'ers on this list,
Can this be done using either a NSOM probe or an NSOM-like input
probe? If so, this would likely have many uses.
Yours,
Bill Tivol




From: John Getty :      jgetty-at-du.edu
Date: Thu, 25 Jan 1996 11:44:19 -0700 (MST)
Subject: Call for Papers, Denver X-Ray Conference

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CALL FOR PAPERS (December 1995)
(Deadline for Submission of Abstracts is April 1, 1996)

=09COMBINED MEETING
=0945th ANNUAL DENVER X-RAY CONFERENCE
=09=09- and -
=09POWDER DIFFRACTION SATELLITE MEETING OF THE XVII=20
=09CONGRESS OF THE INTERNATIONAL UNION OF CRYSTALLOGRAPHY

August 3-8, 1996, Denver, Colorado, U.S.A.

Co-Sponsored by the IUCr; the ICDD; and the University of Denver=20
Department of Engineering=20

=09The meeting will be held August 3-8, 1996, at the=20
Marriott Denver Tech Center Hotel,=20
4900 S. Syracuse St., Denver, CO 80237, U.S.A. =20
Phone (303) 779-1100, Fax (303) 740-2523. =20

Prospective attendees should make reservations early. The number of=20
rooms at the special conference rate is limited. The Conference rate=20
of $76.00 per day plus 11.8% tax for a single or double room can be=20
obtained if reservations are made before July 1, 1996, subject to=20
availability. Also available are a limited number of rooms for=20
students at $22.50 per night (double occupancy).=20

The Conference has appointed Monarch Travel as the official travel=20
agency to help with travel and car rental arrangements. Please contact=20
Monarch Travel by phone at (800) 458-7177, or (215) 557-7177=20
outside the U.S., or fax (215) 563-0479, with travel questions. The=20
program is tentatively as follows:

PLENARY SESSION =20
=09GRAZING-INCIDENCE X-RAY ANALYSIS
Organizers:=09T. C. Huang, IBM Almaden Research Center, San Jose, CA=20
=09=09R. J. Cernik, Daresbury Lab, Warrington, England
Opening remarks: by P. K. Predecki, University of Denver
Presentation of the 1996 Birks Award: to John V. Gilfrich, SFA,=20
=09Inc./NRL, Washington, DC
Presentation of ICDD Distinguished Fellow Awards: to J. W. Visser,=20
=09Delft, Netherlands; and D. K. Smith, =09Pennsylvania State University

INVITED PAPERS:
"Grazing Incidence Small-Angle X-Ray Scattering," J. B. Cohen,=20
=09Northwestern Univ., Evanston, IL

"Depth Scaling of Phases in Thin Films," H. Goebel, Siemens AG=20
=09Corp., Munich, Germany=20

"Grazing-Incidence Technique for Surface, Interface, and Thin Film=20
=09Analysis," T. C. Huang,=20
=09IBM Almaden Research Lab, San Jose, CA; and=20
=09P. K. Predecki, Univ. of Denver, CO=20

"Grazing Angle X-Ray Spectrochemical Analysis, Present and=20
=09Future," Y. Gohshi, Univ. of Tokyo=20

"Analysis of Layered Materials Using Glancing-Incidence X-Ray=20
=09Reflection," D.K.G. de Boer and =09
=09A.J.G. Leenaers, Philips Research Labs, Eindhoven,=20
=09The Netherlands


SPECIAL SESSIONS=20


- PHASE QUANTIFICATION (CPD sponsored)
Organizers: D. L. Bish, Los Alamos National Lab, NM (505) 667-1165,=20
=09=09fax (505) 665-3285
=09=09D. K. Smith, Pennsylvania State University, PA (814) 865-5782,=20
=09=09fax (814) 863-7845

INVITED PAPERS:
"Quantitative Phase Analysis Using the Full Powder Diffraction=20
=09Pattern - Its Development and Current Status,"=20
=09R. J. Hill, CSIRO, Port Melbourne, Australia


- THIN FILMS AND MULTILAYERS (CPD sponsored)
Organizers: H. Goebel, Siemens AG, Germany fax 49 89 636 42256, =20
=09=09herb.goebel-at-zfe.siemens.de
=09=09D. E. Cox, Brookhaven National Lab, NY (516) 282-3818,=20
=09=09fax (516) 282-2739

INVITED PAPERS: (for Thin Films and Multilayers session)
"The Domain Structure of Langmuir-Blodgett Multilayers," U.=20
=09Pietsch, Univ. of Potsdam, Germany
"X-Ray Analysis of Magnetic Multilayers," V. Valvoda, Charles=20
=09Univ., Prague, Czech Republic
"Quantitative Characterization of Textures in Thin Films:=20
=09Measurements and Analysis," H. R. Wenk,=20
=09Univ. of California, Berkeley


- QUANTITATIVE XRF
Organizers: =09V. E. Buhrke, The Buhrke Company, Portola Valley,=20
=09=09CA (415) 851-5020, fax (415) 851-3696=20
=09=09H. Ebel, Technische Universit=E4t Wien, Vienna, Austria=20
=09=09fax 43 158 78 876

INVITED PAPERS:
"Routine X-Ray Fluorescence Spectrometric Analysis by VERBA-
=09XRF," P. Verkhovodov, Kiev, Ukraine
"The Permutations and Combinations of Quantitative XRF=20
=09Methods," G. Lachance, Hammond, Ont., Canada
"An Analysis of Secondary Enhancement Effects in Quantitative=20
=09XRFA," M. Mantler, Technical Univ. of Vienna


- TOTAL REFLECTION XRF
Organizers: M. A. Zaitz, IBM East Fishkill, NY (914) 894-6337,=20
=09fax (914) 892-6256 =20
=09=09A. Shimazaki, Toshiba Corp.

INVITED PAPERS:
"A Review on Principles and Recent Developments in TXRF," H.=20
=09Aiginger, Atominstitut, Vienna, Austria
"New Possibilities and Applications of Total Reflection X-Ray=20
=09Fluorescence Analysis," A. Knochel,=20
=09University of Hamburg, Germany


- STRAIN/STRESS DETERMINATION BY DIFFRACTION METHODS
Organizers:=09A. Winholtz, University of Missouri (314) 882-6322,=20
=09=09fax (314) 884-5090
=09=09T. Ericsson, University of Linkoping, Sweden fax 46 1328 2505

INVITED PAPERS:
"Dependence of the Elastic Constants for X-Ray Diffraction on the=20
Diffraction Plane,"=09I. C. Noyan,=20
=09IBM, Yorktown Heights, NY; and C. C. Goldsmith,=20
=09IBM East Fishkill Facility, NY.
"Partially Destructive and Non-Destructive Determination of=20
=09Residual Stress States with Steep Subsurface Gradients,"
=09B. Eigenmann, Univ. Karlsruhe, Germany
"Second Order Stresses in Single Phase and Multiphase Materials:=20
=09Examples of Experimental and Modeling =09Appproaches," J. L.=20
=09Lebrun, ENSAM, Paris, France=20


- DIFFRACTION PEAK PROFILE ANALYSIS (CPD sponsored)
Organizers:=09R. L. Snyder, Alfred University, NY (607) 871-2438,=20
=09=09fax (607) 871-2392
=09=09D. Lou=EBr, University of Rennes, France =20
=09=09fax 33 9938 3487, louer-at-cicb.fr

INVITED PAPERS:
"Model-Based Interpretation of Diffraction Line Profiles: The Case=20
=09of Lattice Distortion," E. Mittemeijer,=20
=09Technical University of Delft, The Netherlands
"Model-Based Interpretation of Diffraction Line Profiles:=20
=09Determination of Crystallite Size and Shape,"=20
=09I. Langford, University of Birmingham, U.K.
"Model-Based Interpretation of Diffraction Line Profiles: Strain=20
=09Broadening Caused by Dislocations," T. Ungar, =09Eotvos=20
=09University, Budapest, Hungary


- ENVIRONMENTAL APPLICATIONS OF XRF
Organizers:=09R. Jenkins, ICDD, PA (610) 325-9810, fax (610) 325-9823
=09=09Y. Gohshi, University of Tokyo, Japan fax 81 3 3812 9254

INVITED PAPERS:
"Use of XRF for Analysis of Contaminants in Seawater," T. Elam,=20
=09NRL, Washington, DC
"Use of Semi-Quantitate Analysis Programs in the Analysis of=20
=09Pollutants," J. Croke, Philips Electronic=20
=09Instruments, Mahwah, NJ
(Title to be announced) Y. Gohshi, University of Tokyo, Japan


- NEW DEVELOPMENTS IN DETECTORS AND OTHER X-RAY INSTRUMENTATION =09
=09(CPD sponsored)
=09Organizers: H. Toraya, Nagoya Inst. of Technology, Japan =20
=09fax 81-572-27-6812, toraya-at-crl.nitech.ac.jp
=09=09J. V. Gilfrich, SFA, Inc./NRL, Washington, DC (301)=20
=09=09365-5070 fax & phone

INVITED PAPERS: (for New Developments in Detectors session)
=09"Novel Use of Imaging Plates in Powder Diffraction on the=20
=09Australian National Beamline Facility," D. Cookson, =09
=09Australian Nuclear Science and Technology=20
=09
=09"High Energy Resolution X-Ray Detectors Using Superconductors,"=20
=09D. Van Vechten
=09
=09"Multiple-Detector System for Powder Diffraction with Synchrotron=20
=09Radiation," H. Toraya,=20
=09Nagoya Inst. of Technology, Japan.


- PRECISION AND ACCURACY IN STRUCTURE REFINEMENT FROM POWDER=20
DATA (CPD sponsored)
Organizers:=09W.I.F. David, Rutherford-Appleton Lab, Chilton,=20
=09=09U.K. wifd-at-isise.rl.ac.uk =20
=09 R. A. Young, Georgia Inst. of Technology, Atlanta =20
=09=09(404) 894-5208, r.young-at-physics.gatech.edu=20

INVITED PAPERS:
"Optimised Data Collection and Refinement Strategies for Powder=20
=09Data Analysis," W.I.F. David,=20
=09Rutherford-Appleton Lab, Chilton, U.K.
=09"Structure Refinement with Combined X-Ray and Neutron=20
=09Diffraction Data - Successes and Failures,"=20
=09R. B. Von Dreele, Los Alamos National Lab, NM=20


- XRF ANALYSIS OF SEMICONDUCTOR WAFERS (BPSG)
=09Organizers: R. Wilson, Rigaku/USA, Danvers, MA (508) 777-
=092446 x124, fax (508) 777-3594
=09=09A. Iida, Photon Factory, Ibaraki, Japan =20
=09=09aiida-at-kekvax.kek.jp

INVITED PAPERS:
"XRF Analysis of Thin Films and Problem Solving in the=20
=09Semiconductor Industry," M. A. Zaitz,=20
=09IBM, East Fishkill, NY.
"Measurement Capabilities of X-Ray Fluorescence for BPSG=20
=09Films," J. Westphall,=20
=09Watkins-Johnson Company, Scotts Valley, CA
"XRF Analysis of Insulator Films on Si Substrate," T. Konishi,=20
=09Asahi Chemical Industry Co., Japan


- HIGH-TEMPERATURE AND NON-AMBIENT APPLICATIONS OF DIFFRACTION
Organizer: C. R. Hubbard, Oak Ridge National Lab, TN =20
=09(423) 574-4472, hubbardcr-at-ornl.gov

INVITED PAPERS:
(To be announced)

TUTORIAL WORKSHOPS


- CREATING WINDOWING INTERFACES FOR FORTRAN=20
=09PROGRAMS: NEW LIVES FOR OLD TOOLS (1/2 day)
=09B. Toby, NIST, Gaithersburg, MD; and L. Finger,=20
=09Geophysical Laboratory, Washington, DC

- TOTAL REFLECTION XRF (one day)
=09P. Wobrauschek, Atominstitut, Vienna

- QUANTITATIVE TECHNIQUES AND ERRORS IN XRF (1/2 day)
=09J. A. Anzelmo, Fisons Instruments

- QUANTITATIVE TECHNIQUES AND ERRORS IN XRPD (1/2 day)
=09R. L. Snyder, Alfred University, NY; and R. Hill, CSIRO,=20
=09Australia

- NEW TECHNIQUES IN SEARCH-MATCHING (ICDD sponsored) (1/2 day)
=09R. Jenkins, ICDD, PA; and D. K. Smith, Penn State=20
=09University

- E-MAIL SYSTEMS AND WWW RESOURCES (x-ray related) (1/2 day)
=09R. Jenkins, ICDD, PA; and L. Cranswick, CSIRO, Australia

- SPECIMEN PREPARATION FOR XRF (one day)
=09V. E. Buhrke, The Buhrke Company, Portola Valley, CA

- ANALYSIS OF METALS BY XRF (1/2 day)
=09F. Feret, Alcan International, Canada

- XRD STANDARDS, CALIBRATION AND ALIGNMENT (1/2 day)
=09J. P. Cline, NIST, Gaithersburg, MD; and R. W. Cheary,=20
=09Univ. of Technology, Sydney, Australia

- TEXTURE AND RELATED 3-D POLYCRYSTAL ANALYSIS (1/2 day)
=09H. Bunge, Technical Univ. Clausthal, Germany; and R. Von=20
=09Dreele, Los Alamos National Lab, NM

- GRAZING INCIDENCE X-RAY CHARACTERIZATION (1/2 day)
=09D. K. Bowen, University of Warwick, U.K.


- MICROANALYSIS APPLICATIONS XRD AND XRF (1/2 day)
=09M. O. Eatough, Sandia National Labs, NM; and J. Brown,=20
=09University of Western Ontario, Canada

- STRATEGY FOR CALIBRATION OF XRF SPECTROMETERS (1/2 day)
=09B. Vrebos, Philips, The Netherlands; and P. A. Pella, NIST,=20
=09Gaithersburg, MD

- METHODS OF CORRECTING FOR MATRIX EFFECTS IN XRF (1/2 day)
=09R. Rousseau, Geological Survey of Canada

- SYNCHROTRON RADIATION ANALYSIS (1/2 day)
=09P. Stephens, State University of New York; and A. Fitch,=20
=09ESRF, Grenoble, France
=20
=09Contributed papers are hereby solicited for any of the above=20
special sessions or the XRD and XRF general sessions. Many of the=20
contributed papers will be placed in poster sessions. Those of more=20
general interest will be placed in oral sessions. =20

=09THE DEADLINE FOR SUBMISSION OF ABSTRACTS IS APRIL 1, 1996. =20

Abstracts are reproduced as-is in the abstracts book. They must not=20
exceed one page in length and must include title, author(s),=20
affiliation(s) and the text. They should be typed single-spaced using a=20
laser printer or equivalent with characters at least 2 mm high (lower=20
case) on a 15 cm wide x 20 cm long image area centered on an 8-1/2"=20
x 11" or A4 page, mailed flat without folds. On a SEPARATE=20
PAGE state: (1) speaker's name, mailing address, phone, fax and e-
mail numbers; and (2) whether you intend to publish this paper in the=20
conference proceedings, Advances in X-Ray Analysis, Vol. 40. The=20
original and one copy of the abstract should be sent to:

=09Paul K. Predecki, Dept. of Engineering, University of=20
Denver, Denver, CO 80208, USA. =20

Additional information may be obtained from Lynne Bonno at the=20
above address, telephone (303) 871-3515, fax (303) 871-4450, or e-
mail denxrcon-at-du.edu. A program for the Conference will be=20
prepared and mailed in May 1996. The program as well as this call for=20
papers will be placed on the University of Denver home page:
=09http://littlebird.engr.du.edu/home.html =20

=09The Organizing Committee considers unprofessional the=20
withdrawal of a paper (except in special circum-stances) after it has=20
been accepted and widely advertised. Non-U.S. authors, in particular,=20
please try to secure travel funding and approvals before submitting=20
your abstract(s).

=09With regard to publication of presented papers, manuscripts=20
must be submitted no later than one month after the conference to=20
allow publication before the next conference. To be acceptable for=20
publication, papers should describe either new methods, theory and=20
applications, improvements in methods or instrumentation, or other=20
advances in the state of the art. Papers emphasizing commercial=20
aspects are discouraged. Information for preparing manuscripts will=20
be mailed in June 1996.




From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Thu, 25 Jan 1996 04:03:37 -0400
Subject: Re: EBSP system

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {n1389523211.42950-at-msmail.tmc.tulane.edu}
"'smtp:Microscopy-at-Msa.Microscopy.Com'" {Microscopy-at-Sparc5.Microscopy.Com}

At 9:13 AM 1/24/96, Griffin, Robin wrote:
} I'm trying to get in touch with TSL TexSEM Laboratories Incorporated a
} company that makes EBSP software for Windows. I have a phone number
} 801-467-9930, but nobody answers. Anyone know how to get in touch with
} them?


Didnt they get bought out by Noran?

I heard a rumor.....

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://wwwpersonal.engin.umich.edu/~jfmjfm/jfmjfm.html






From: Rick Markgraf :      rlmarkgraf-at-ucdavis.edu
Date: Thu, 25 Jan 1996 17:16:55 -0800
Subject: Re: recharge centers

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I recharge for use of specialized optical microscopes. Your University
should have a budget analyst in Budget that can help you.
You need to estimate the amount of non-use you expect and build that
into your rate as an expense. You also charge for your overhead, salaries
(figure in vacation & sick leave, breaks, etc.) and supplies. You also
should calculate depreciation for your equipment and build a reserve so you
can buy new equipment. Our campus even has a high-value equipment reserve
that provides for catastrophic loss of an expensive scope.
When you have figured all your costs, you should be able to divide it
up as fits your program. For instance, charge a one-time lump sum for each
use that recovers costs incurred regardless of the length of use, i.e.
cleaning, prep of scope, etc. Then, in addition, charge a low hourly rate
that covers the variable expenses. Don't forget to find out what the
non-university differential is and mark-up the rate for off-campus
customers. Billing is easier if you automate it by computer and its also
easier to see how you did, so you can adjust your rate next year.
Good Luck and keep good records.

At 07:47 AM 1/24/96 CST, Robin Griffin wrote:
}
} This recharge issue has recently come up here to. We are trying to comply
} with the rules and are looking for ways to do it without hurting the lab.
} My understanding is that
} 1) we need to charge rates that only recover costs (no profit - not ever a
} problem here) and
} 2) we must charge all univerisity users the same rates
}
} I was considering taking the cost of the lab and charging grants a
} percentage, sort of like an overhead that would cover the costs. Our
} accounting department seemed to think this would be okay. I liked it
} because I am worried that if we charge a fixed hourly rate, users would
} minimize lab. use. Many of my expenses are fixed so less use only means
} less bang for the buck. However, the U. of Hawaii writer seemed to think
} this might be illegal. It is also very scary to hear that they will come
} and audit- Eek! How about more specifics about the lump sum charging you
} did that got you in trouble and your experiences with the audit.
}
} Another issue, we have some professors that support the lab when they have
} money but some years they come up dry. We've always given them free time
} during their dry years with the idea that they can't get more grants without
} some work to show. It has always paid off for us. However, this appears to
} be against the recharge center rules because we are charging people with
} grants more than people without.
}
} One other issue, along with doing research, we teach laboratories on our em
} lab instrumentation. Any comments on how or if this is billed? I can't
} find anything in our rules about it.
}
} Thanks
}
} Robin Griffin
} EM Lab Manager
} Materials and Mechanical Engineering
} University of Alabama at Birmingham
}
}
Richard L. Markgraf
Microscope Services
University of California, Davis
Ph. (916)752-3477 Fax (916)752-6363
rlmarkgraf-at-ucdavis.edu





From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Fri, 26 Jan 1996 17:31:27 +1100
Subject: Burleigh Instruments Inc - address anyone?

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X-Sender: st004718-at-brandywine.otago.ac.nz
Message-Id: {v01530501ad2e24ad7ec3-at-[139.80.120.183]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Has anyone out there heard of a company called Burleigh Instruments Inc? If
you have and they are still in existence we would appreciate it if you
could let us know their address or phone number.

Thanks in advance.

Regards
Richard.


Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD















From: Zhiyu Wang :      zhiyu-at-hawaii.edu
Date: Thu, 25 Jan 1996 21:40:59 -1000
Subject: Re: Thin sections of polymers

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Hi, Kathy:

I had some experience in thin sectionning in polymer materials for TEM.

The resin I used is water soluable GMA. The reference can be found on:

Zhiyu Wang etc., The study of microstructure and ultrafiltration membrane
by electron microscope. Preceeding of the 1990 international congress on
membrane and membrane prcess. Vol. II, pp. 1211-1213, Chicago.

Please let me know if you have question.

Zhiyu Wang
Department of Biosystem Engineering
University of Hawaii
Honolulu Hawaii 96822




From: Richard Easingwood
Date: 26 January 1996 01:31
Subject: Burleigh Instruments Inc - address anyon

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Message-ID: {F972E82F01681600-at-c2gate.tcom.co.uk}

Original Subject:
Burleigh Instruments Inc - address anyone?

Has anyone out there heard of a company called Burleigh Instruments Inc? If
you have and they are still in existence we would appreciate it if you
could let us know their address or phone number.

Thanks in advance.

Regards
Richard.


Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD















From: tom thielen :      thielent-at-lurch.winthrop.edu
Date: Fri, 26 Jan 1996 09:37:33 -0500 (EST)
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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please "unsubscribe" me
-thanks

*******************************************************************************
*Tom Thielen "People who make sweeping generalizations are stupid!" *
*Pre-Med Major *
*Winthrop University thielent-at-lurch.winthrop.edu *
*******************************************************************************








From: keller-at-boulder.nist.gov (Bob Keller)
Date: Fri, 26 Jan 1996 07:50:02 -0700
Subject: EBSP system - TSL

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X-Sender: keller-at-arc1.mrd.bldrdoc.gov
Message-Id: {v01510106ad2e99b91186-at-[132.163.192.156]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} At 9:13 AM 1/24/96, Griffin, Robin wrote:
} } I'm trying to get in touch with TSL TexSEM Laboratories Incorporated a
} } company that makes EBSP software for Windows. I have a phone number
} } 801-467-9930, but nobody answers. Anyone know how to get in touch with
} } them?
}
}
} Didnt they get bought out by Noran?
}
} I heard a rumor.....
}
} John Mansfield

They weren't bought out by Noran, but rather Noran is marketing their
systems. You can still deal directly with the TSL people if you want.

Bob Keller
NIST Mat'ls. Reliability Div.
Boulder, CO






From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Fri, 26 Jan 1996 09:30:17 -0700
Subject: Re: Thin sections of polymers

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} Esteemed Colleagues:
}
} I have been asked to image the interior structure of a polymer
} suspension.
}

**specifics deleted**

Any thoughts on the usefulness of freeze fracture to get a look at the
internal structure? I'm not a polymer person either, so don't have any
experience with this. You could, perhaps, avoid chemical changes with a
freezing technique.

John
chandler-at-lamar.ColoState.EDU






From: vskulkar-at-wolf.co.net (Vitthal Kulkarni)
Date: Fri, 26 Jan 1996 12:13:05 -0600
Subject: fluorescence-microscope

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We are interested in setting up a Langmuir trough with fluorescence microscope.
Currently, we are gathering information on fluorescence microscopes used for
the studies of two-dimensional phase states of Langmuir monolayers at
air/water interface. The microscopes are mounted on Langmuir trough and
phase changes in lipid monolayers can be monitored with compression of the
film. [e.g see reference K.J. Stine and C.M. Knobler, Ultramicroscopy 47,
23-34 (1992)]

Any information regarding types of fluorescence microscopes used in such
studies, their resolution, particular requirements, and accessories, etc
will be of great help.
Thank you,
Vitthal
Vitthal Kulkarni, Ph.D.
Membrane Biochemistry
The Hormel Institute
University of Minnesota
801, 16th Avenue NE
Austin, MN 55912
Tel. 507-437-9626
Fax. 507-437-9606





From: Dr. Andrew P. Somlyo :      aps2n-at-elvis.med.virginia.edu
Date: Fri, 26 Jan 1996 09:49:34 -0500 (EST)
Subject: Re: LM: uncaging instrumentation

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For general information, see:



J. A. Mccray D. R. Trentham
Properties and uses of photoreactive caged compounds
Annu. Rev. Biophys. Chem.
1989
18: 239 270


On Thu, 25 Jan 1996, William Tivol wrote:

} } I wonder what equipment is used or should be used for uncaging (photolysis)
} } of caged (photoactivable) compounds.
} } [snip]
} } - What light sources are used (types of lasers, Xenon arc lamps, Mercury arc
} } lamps, flash lamps)?
} }
} } - How is the exact light path to the cell/cells/subcells? What types of
} } optics and components are used?
} } [snip]
}
} Dear Martin and especially SPM'ers on this list,
} Can this be done using either a NSOM probe or an NSOM-like input
} probe? If so, this would likely have many uses.
} Yours,
} Bill Tivol
}




From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Fri, 26 Jan 1996 08:42:04 -0600
Subject: MSA/MAS/MSC-SMC Joint Meeting 1996

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G'day Subscribers....

I've gotten several off-line inquires about the
Microscopy & Microanalysis - 1996 which is being held in Minneapolis
on August 11-15,1996, and is the joint annual meeting
sponsored by MSA, MAS, MSC-SMC . Many have asked about the fact that
they have not yet received registration information.

I am posting this just to reassure all of you, that you will receive
relevant the information, albeit slightly late this year.
The registration booklet and call for papers is behind
schedule this year and we anticipate it being shipped within 2 weeks.
As soon as the booklets are ready for shipping I will post another
announcement to let people know that they are "in-the-mail".

The information will also be put up on the MSA WWW site, probably
by the middle of next week. However, you will not be able to
download the various manuscript forms, since they will not be suitable
for publication.

Abstracts of the Major Symposia and Topics for contributed papers/posters
are already on that site (http://www.msa.microscopy.com) under the
Annual Meetings Topic and you can use that information to start
planning your manuscripts and which session you would like to submit them to.

The deadline for receipt of Abstracts remains March 15th
as in previous years. Registration can be of course accepted right up
to the day of the meeting, by snail-mail, fax, phone, or in-person.


Cheers...
Nestor
Your Friendly Neighborhood SysOp
&
Microscopy & Microanalysis - 1996 Program Chair






From: Richard Thrift :      Richard_Thrift-at-depotech.com
Date: Fri, 26 Jan 1996 12:54:29 -0800
Subject: fluorescence-microscope -Reply

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Message-Id: {s108cf1d.051-at-depotech.com}
X-Mailer: Novell GroupWise 4.1

This is a good question to post to the microscopy listserver:
Microscopy-at-MSA.Microscopy.Com
(ask to have replies sent directly to you if you're not a subscriber).
To subscribe, I think you send the subject & text "subscribe" to
Listserver-at-MSA.Microscopy.Com (They've changed it since I
subscribed.)

Richard Thrift
p.s. again, I'm interested in the response you get. Thanks for providing
the reference.

} } } Vitthal Kulkarni {vskulkar-at-wolf.co.net} 01/26/96 10:13am } } }
We are interested in setting up a Langmuir trough with fluorescence
microscope.
Currently, we are gathering information on fluorescence microscopes
used for the studies of two-dimensional phase states of Langmuir
monolayers at air/water interface. The microscopes are mounted on
Langmuir trough and phase changes in lipid monolayers can be
monitored with compression of the film. [e.g see reference K.J. Stine and
C.M. Knobler, Ultramicroscopy 47,
23-34 (1992)]

Any information regarding types of fluorescence microscopes used in
such studies, their resolution, particular requirements, and accessories,
etc will be of great help.
Thank you,
Vitthal
Vitthal Kulkarni, Ph.D.
Membrane Biochemistry
The Hormel Institute
University of Minnesota
801, 16th Avenue NE
Austin, MN 55912
Tel. 507-437-9626
Fax. 507-437-9606







From: wise-at-vaxa.cis.uwosh.edu
Date: Fri, 26 Jan 1996 10:21:43 +0000
Subject: Re: Burleigh Instruments Inc - address anyone?

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} Has anyone out there heard of a company called Burleigh Instruments Inc? If
} you have and they are still in existence we would appreciate it if you
} could let us know their address or phone number.
}
} Thanks in advance.
}
} Regards
} Richard.
}
}
} Richard Easingwood
} South Campus Electron Microscope Unit
} Otago Medical School
} PO Box 913
} Dunedin
} NEW ZEALAND
}
} Telephone: 64-03-479 7301
} Facsimile: 64-03-479 7254
}
} SOUTHERNMOST E.M UNIT IN THE WORLD

Try

Burleigh Instruments Inc
Burleigh Park
Fishers, NY 14453
(716) 924-9355 fone
(716) 924-9072 fax
97-8379 tlx

UK
Burleigh Inst Ltd
Nine ALlied Business Centre
Cold Harbor Lane
Harpenden, Herts, AL5 4UT
(0582) 766888
(0582) 767888 fax

Europe
Burleigh Inst GmbH
Bergstrasse 104-106, D-6102
Pfungstadt, Germany
(06157) 3047
(06157) 7530 fax

Japan
Techscience Ltd
0489 (64) 3111
0489 (65) 1500

Burleigh has written a very useful 17 page booklet that explains the basic
of scanning probe microscopy called the Scanning Probe Microscope Book
(part number (?) STM 364 493, 51993-0). I have found it to be a great
intro to SPM.

Bob



Robert R. Wise, PhD
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: John_R_Reffner-at-rohmhaas.com (John R Reffner)
Date: 1/25/96 2:36 PM
Subject: Thin sections of polymers

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Mime-Version: 1.0

A technique we use to look at larger polymer particles that are sensitive to
embedding in epoxies is to blend them with a film-forming latex, cast films
and section.


-| Dr. John R. Reffner | email: rsrj2r-at-rohmhaas.com |-
-| --------------------------------------------------------------|-
The opinions expressed are those of the writer and not Rohm and Haas Company
________________________________________________________________



Esteemed Colleagues:

I have been asked to image the interior structure of a polymer
suspension.

Ideally I would like to resin embed for TEM. I am not a polymer
scientist, but I do know that these polymers produce spheres when
suspended with small amounts of BSA. The polymer is polyglycolic acid
(PGLA). I called the manufacturers, but they have not done any
significant imaging of it, and do not appear to be interested. They did
tell me that the PGLA is heat sensitive and dissolves in isopropal
alcohol. My experiment with epoxys was an unmitigated disaster, and I am
considering a low temperature embedment procedure (Another researcher
tried Lowicryl, but the spheres did not remain intact).

I referenced a book entitled "Polymer Microscopy" by Sawyer and
Grubb, but it doesn't refer to anything similar to my polymer.

Any (relevent) thoughts would be appriciated.

Kathy Walters
CMRF
U of Iowa





From: CHAFFEYN :      NIGEL.CHAFFEY-at-bbsrc.ac.uk
Date: Fri, 26 Jan 1996 17:03:14 +0000
Subject: Toluidine blue-quenching of autofluorescence - how?

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Via: uk.ac.bbsrc; Fri, 26 Jan 1996 17:03:27 +0000
X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; Relayed;
Fri, 26 Jan 1996 17:05:10 +0000
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Fri, 26 Jan 1996 17:03:14 +0000

Dear Fellow Microscopists,
Happy New Year to you all (not too late I hope!).

I routinely use a dilute solution of toluidine blue to quench some of
the autofluorescence present in my material prepared for indirect
immunofluorescence microscopy of tubulin. I am convinced that it does reduce
the native fluorescence of my tissue (secondary vascular tissue of horse
chestnut). However, I was floored at question time at the end of a talk at an
international conference when asked, 'how does the quenching work?'. I have
asked colleagues and consulted textbooks, but still do not have the answer.
Can anybody help, please? [I'm sure that having the answer the question will
never be asked again, but it might!]

Many thanks,

Nigel Chaffey: IACR - Long Ashton Research Station, Bristol BS18 9AF,
UK [the Long Ashtonmost EM unit in the universe]




From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Fri, 26 Jan 1996 19:28:44 -0800 (PST)
Subject: Re: Low viscosity nitrocellulose

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Message-Id: {199601262022.OAA12999-at-alpha2.csd.uwm.edu}

Brett,

You can Low Viscosity Nitrocellulose from:
Randolph Products
Carlstadt, NJ 07072

Regards,
Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu






From: SALLY STOWE :      stowe-at-rsbs-central.anu.edu.au
Date: Sat, 27 Jan 1996 14:25:28 EST10
Subject: Re: Toluidine blue-quenching of autofluorescence - how?

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Nigel Chaffey wrote:}
} I routinely use a dilute solution of toluidine blue to quench some of
} the autofluorescence present in my material prepared for indirect
} immunofluorescence microscopy of tubulin. I am convinced that it does reduce
} the native fluorescence of my tissue (secondary vascular tissue of horse
} chestnut). However, I was floored at question time at the end of a talk at an
} international conference when asked, 'how does the quenching work?'. I have
} asked colleagues and consulted textbooks, but still do not have the answer.
} Can anybody help, please? [I'm sure that having the answer the question will
} never be asked again, but it might!]
}
} Many thanks,
}
} Nigel Chaffey: IACR - Long Ashton Research Station, Bristol BS18 9AF,
} UK [the Long Ashtonmost EM unit in the universe]


Brief rinsing with a very dilute solution of tol. blue also works
to quench autofluorescence in Drosophila eyes, using thin frozen
sections and FITC labelling. I'm not sure of the original rationale
-the autofluorescence is very high, we were desperate for something
that would work. I think just a hope that tol. blue probably binds to
double bonds (??) and might just dampen things down..and so it did.
Dont think I ever tried it with either rhodamine or a
short-wavelength chromophore like DAPI ...does tol blue itself
fluoresce under some excitation wavelengths?

Sally Stowe
Australian National University EM Unit, Canberra. (28 degrees centigrade,
blue skies, cool breeze..)
----------------------------------------------------------------------
Sally Stowe |Email: stowe-at-rsbs.anu.edu.au
Facility Coordinator |Post:
ANU Electron Microscopy Unit |ANUEMU (RSBS)
Ph 61 6 249 2743 |Australian National Univ.
FAX 61 6 249 4891 |Canberra,
|AUSTRALIA 0200






From: Richard Abbott :      abbott-at-ligo.caltech.edu (by way of zaluzec-at-Microscopy.Com (Nestor J. Zaluzec))
Date: Sat, 27 Jan 1996 13:38:59 -0600
Subject: My Microscope

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Some time ago I purchased a beautiful microscope. One of the lenses
is an oil immersion type using "Cedar Oil". In a moments foolish
enthusiasm, I immersed the lens in the oil and used it to my delight.
Only after I was done did I realize that I have no idea how to clean
the lens. Now the lens has a gummy oil residue left on it rendering
it useless to me. Can anyone tell me how to clean my lens? I would
be delighted to get an answer.

Thanks, Rich Abbott.









From: Peter D. Barnett :      pbarnett-at-crl.com
Date: Sat, 27 Jan 1996 14:37:54 -0800
Subject: parts for B&L Model L

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I recently obtained a B&L Model L photomicrographic camera with a bunch of
lenses, base, bench, and accessories. It is equipped with a head which has
a reflex viewer and accomodates 5"x7" film holders. I would like to be able
to use this with 4"x5" film holders and thought perhaps someone on this list
might have an old one around with some parts they'd be willing to part with.

Or, maybe someone can suggest where to get a reducing back to go from a
5"x7" to 4"x5" film holder.

If so, please drop me a note directly.

Thanks.
Peter D. Barnett
Forensic Science Associates - Richmond CA
e-mail: pbarnett-at-crl.com FAX_510-222-8887





From: xin yang li :      xl48-at-uow.edu.au
Date: Mon, 29 Jan 1996 10:05:36 +1100 (EST)
Subject: Unsubscribe

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Could you please remove my name from subscription list?

Thanks




From: Finn-Mogens Haug :      f.m.s.haug-at-basalmed.uio.no
Date: Sun, 28 Jan 1996 10:38:47 +0100
Subject: Users/testers of SENSYS or Micromax cameras

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Message-Id: {199601290933.KAA23689-at-pons.uio.no}
X-Sender: finnmog-at-pons.uio.no
X-Mailer: Windows Eudora Pro Version 2.1.2
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

know that they are new, more moderately priced, cooled,
slow-scan cameras from Photometrics and Princeton. (The Photometrics
SENSYS was announced last November and may only now be produced
in volume - but it has been demonstrated in the US, I believe. The Princeton
Micromax is a packaging of the Small cooled camera head and the
ST133 controller).

If you have used or tested one or both for (biological) fluorescence
microscopy, I should be grateful for the opportunity to put some practical
questios, before deciding whether to go for one of them. (For the Princeton
cameras, experience with the TE cameras and ST133/ST133 controllers is
probably equally useful.)

Best regards, Finn-Mogens
*****************************************************************
Finn-Mogens Haug
University of Oslo, Institute of basic medical sciences,
Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Box 1105 Blindern Phone : +47 22 85 12 67
N-0317 Oslo, NORWAY Fax : +47 22 85 12 78
*****************************************************************






From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 29 Jan 1996 12:05:46 -0500 (EST)
Subject: Re: Toluidine blue-quenching of autofluorescence - how?

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} [snip] However, I was floored at question time at the end of a talk at an
} international conference when asked, 'how does the quenching work?'. I have
} asked colleagues and consulted textbooks, but still do not have the answer.

Dear Nigel,
Fluorescence is the radiative decay of an excited state of a molecule
to (usually) the ground state. Other decay modes exist and are always in
competition with radiative decay--thus the fluorescence yield term in inten-
sity formulas. Quenchers enhance non-radiative decay either by being accep-
tors for energy transfer or by promoting collisional de-excitation, and by-
and-large the energy transfer process is the most important. In this process,
a nearby quencher molecule is converted into an excited state and the fluo-
rescent molecule is converted into the ground state in a single step. The
quencher then decays via a non-radiative process.
Yours,
Bill Tivol




From: dbd1-at-uclink4.berkeley.edu
Date: Mon, 29 Jan 1996 10:55:46 -0800
Subject: ISI SEM parts needed

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psic-at-uclink4.berkeley.edu

The ISI (Topcon) DS-130 SEM at UC Berkeley has expired. Parts, i.e.
electronics are needed.
If you have or know of a DS-130 scope in storage or in a salvage state,
please contact me.
Thank you.


Doug Davis
Staff Research Associate
Electron Microscope Facility
University of California
Berkeley, CA 94720
(510) 642-2085
dbd1-at-uclink4.berkeley.edu







From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 29 Jan 1996 15:05:30 -0400
Subject: RE-Cleaning Oil Imms Lens

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Message-ID: {n1389194398.83112-at-mse.engin.umich.edu}

Subject: Time: 2:47 PM
OFFICE MEMO RE:Cleaning Oil Imms Lens Date: 1/29/96

We always used to use a lens tissue moistened with a bit of xylene to clean
the oil off oil immersion lenses, and this is the procedure recommended in an
article, "Immersion oil and the microscope", by J. R. Cargille, President of
Cargille Laboratories, the outfit that makes most immersion oils. In his
article he points out that synthetic oils such as those manufactured by the
Cargille Co. have a number of advantages over Cedar oil and other natural
oils (i.e. they contain no volatiles, do not degrade from exposure to light
and normal temperatures, etc.) If you'd like a copy of this article, send me
your FAX number and I'll send it off to you. In any event, I think you
should be particularly careful not to use acetone or alcohol, because they
will soften and dissolve the cement that is usually used to hold the lenses
in place. Best of all, check with the manufacturer of the lens.





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 29 Jan 1996 16:35:03 -0400
Subject: Reitveld Method

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X-Nupop-Charset: English

Subject: Time: 4:29 PM
OFFICE MEMO Reitveld Method Date: 1/29/96

Does anyone know of Macintosh or IBM PC software that is readily available
(preferably free) for determination of lattice parameters from unknown x-ray
powder patterns by the Rietveld method?
W. C. Bigelow (bigelow-at-umich.edu)





From: tsi-at-werple.mira.net.au (Thomson Scientific:Paul Thomson)
Date: Tue, 30 Jan 1996 09:30:25 +1100 (EST)
Subject: Automatic LN Refill systems

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Does anyone know of a commercial vendor of an Automatic LN Refill system?


Regards,


Paul Thomson
Thomson Scientific Instruments





From: lporter-at-goodyear.com (LE Porter)
Date: Mon, 29 Jan 1996 13:23:35 -0500
Subject: Re: Thin sections of polymers

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X-Sender: t456b19-at-rds163
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} Esteemed Colleagues:
}
} I have been asked to image the interior structure of a polymer
} suspension.
}
} Ideally I would like to resin embed for TEM. I am not a polymer
} scientist, but I do know that these polymers produce spheres when
} suspended with small amounts of BSA. The polymer is polyglycolic acid
} (PGLA). I called the manufacturers, but they have not done any
} significant imaging of it, and do not appear to be interested. They did
} tell me that the PGLA is heat sensitive and dissolves in isopropal
} alcohol. My experiment with epoxys was an unmitigated disaster, and I am
} considering a low temperature embedment procedure (Another researcher
} tried Lowicryl, but the spheres did not remain intact).
}
} I referenced a book entitled "Polymer Microscopy" by Sawyer and
} Grubb, but it doesn't refer to anything similar to my polymer.
}
} Any (relevent) thoughts would be appriciated.
}
} Kathy Walters
} CMRF
} U of Iowa


Kathy,

Have you seen the article:"Cryo-Ultramicrotomy of Individual Latex
Particles
for Examination of Internal Morphology" by Angela M Marcelli? It was published
in "The Microscope Vol 43:3 117-120 (1995)". If you supply your fax # I
will
fax you a copy.

L E Porter Phone (216) 796-1620
Head of Microscopy Fax (216) 796-3304
The Goodyear Tire & Rubber Company EMail LPORTER-at-GOODYEAR.COM
Dept 415A
142 Goodyear Blvd
Akron, OH 44305
USA







From: Michael OKeefe :      Michael_OKeefe-at-macmail.lbl.gov
Date: 29 Jan 1996 13:47:28 U
Subject: NCEM Visiting Scientist Pro

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Message-Id: {n1389199025.43681-at-macmail.lbl.gov}



University of California
Lawrence Berkeley National Laboratory
Materials Sciences Division


National Center for Electron Microscopy
Visiting Scientist Program


The National Center for Electron Microscopy is offering a fellowship
that will allow participants the opportunity to conduct research in their
own area of interest using the advanced transmission electron microscopes
at the Center.

The program is intended primarily for young faculty/investigator
electron microscopists, resident in the U.S. who are in the process of
setting up their own facilities or are awaiting delivery of new equipment,
and who could benefit from the head-start that use of instrumentation and
interaction with personnel at NCEM would bring. However, other post-
doctoral applicants with suitable experience and graduate students at an
advanced stage of their thesis work would also be considered. Awards will
be made according to the recommendations of the NCEM Steering Committee.

Fellowships will be of up to three-months duration and will carry a
stipend of up to $6,000 to assist in defraying travel and living expenses.
Applications must be received by March 31, 1996.

For further information and to receive application forms, contact


Gretchen Hermes, Coordinator
National Center for Electron Microscopy, Bldg. 72
Lawrence Berkeley National Laboratory
Berkeley, CA 94720
Tel.: (510) 486-5006
Fax: (510) 486-5888
Email: ghermes-at-lbl.gov






From: PHOBOS11-at-aol.com
Date: Mon, 29 Jan 1996 20:59:59 -0500
Subject: Re: Automatic LN Refill systems

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Hi all,

I saw a company at MSA-95 called VBS. They were marketing some very nice
system. Please check the exhibit directories.

Best Regards,

Al Coritz
RMC




From: Michael OKeefe :      Michael_OKeefe-at-macmail.lbl.gov
Date: 29 Jan 1996 13:50:05 U
Subject: NCEM Visiting Scientist Pro

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Message-Id: {n1389198906.51441-at-macmail.lbl.gov}



University of California
Lawrence Berkeley National Laboratory
Materials Sciences Division


National Center for Electron Microscopy
Visiting Scientist Program


The National Center for Electron Microscopy is offering a fellowship
that will allow participants the opportunity to conduct research in their
own area of interest using the advanced transmission electron microscopes
at the Center.

The program is intended primarily for young faculty/investigator
electron microscopists, resident in the U.S. who are in the process of
setting up their own facilities or are awaiting delivery of new equipment,
and who could benefit from the head-start that use of instrumentation and
interaction with personnel at NCEM would bring. However, other post-
doctoral applicants with suitable experience and graduate students at an
advanced stage of their thesis work would also be considered. Awards will
be made according to the recommendations of the NCEM Steering Committee.

Fellowships will be of up to three-months duration and will carry a
stipend of up to $6,000 to assist in defraying travel and living expenses.
Applications must be received by March 31, 1996.

For further information and to receive application forms, contact


Gretchen Hermes, Coordinator
National Center for Electron Microscopy, Bldg. 72
Lawrence Berkeley National Laboratory
Berkeley, CA 94720
Tel.: (510) 486-5006
Fax: (510) 486-5888
Email: ghermes-at-lbl.gov






From: grial-at-relay.starnet.net.ar
Date: 01/30/96 Time: 08:35:52
Subject: LM - Stores in UK

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From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Tue, 30 Jan 1996 08:44:32 -0600
Subject: RE-Cleaning Oil Imms Lens

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Message-Id: {v01520d01ad33de70f660-at-[128.206.15.200]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Greetings,
Will Bigelow wrote:

} [snip]... In any event, I think you
} should be particularly careful not to use acetone or alcohol, because they
} will soften and dissolve the cement that is usually used to hold the lenses
} in place. Best of all, check with the manufacturer of the lens.

What is the reason for xylene being less likely to dissolve cement
than acetone or ethanol? I would have thought that it depended on the
cement and that generalizations would be difficult. Thanks for any insight
on this.

Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: Michael L. Boucher :      BOUCHER-at-tcrca.usbm.gov
Date: Tue, 30 Jan 1996 09:45:06 CST
Subject: Re: RE-Cleaning Oil Imms Lens

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We've used xylol for the last 30 years here with no serious
consequences on any of our Zeiss and Leitz objectives. The service
people use a combination of ether and other stuff, but we don't need
it often enough to deal with having a can of ether on hand. Xylol
should work fine, but never use alcohol or acetone on objectives.
Mike
*****************************************************************
Michael L. Boucher Sr. Boucher-at-TCRCA.USBM.GOV
Geology-Mineralogy/Chemistry Labs Ph 612-725-4614
Twin Cities Research Center Fax 612-725-4527
U.S. Bureau of Mines Center 725-4500
Department of Interior
5629 Minnehaha Avenue South
Minneapolis, MN 55417-3099
U.S.A.
*****************************************************************





From: Theresa A. Fassel :      fassel-at-post.its.mcw.edu
Date: Tue, 30 Jan 1996 10:12:16 -0600 (CST)
Subject: database for microscopy

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Does anyone know of database software that I could use to (1) input the
form our lab uses for each sample that comes in (2) search the records by
chosen field of information like "cell line" or "users name" for example
and (3) build a new file with the records from that search? This would
enable me to answer questions like "what were all the experiments done on
x cell line from y time frame?" or place all the records from one user
in one file that I could then print or give to him/her. I would also
like to make a rapid index with just the identification number, date,
user and cell or tissue type, as a rapid frame of reference.

Is there software to do this? Any recommendations or experiences?

Theresa

Dr. Theresa A. Fassel
Sr. Research Associate fassel-at-post.its.mcw.edu
Department of Microbiology (414)-456-8410
Medical College of Wisconsin Fax (414)-266-8522
8701 Watertown Plank Road
Milwaukee, WI 53226-0509






From: Jun Jiao :      junj-at-u.Arizona.EDU
Date: Tue, 30 Jan 1996 10:33:55 -0700 (MST)
Subject: subscribe

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Subscribe!





From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Tue, 30 Jan 1996 12:30:21 -0500 (EST)
Subject: workshops

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light and electron microscopists;

I was wondering if anyone knows of any upcoming workshops in molecular
biology using molecular probes for confocal and electron microscopy. I
prefer a workshop where "hands on" techniques are emphasized.

Also, does anyone know where 22mm square, quartz coverslips can be purchased?

Thanks in advance.

Peace,

Phil Rutledge




From: nkrsmith-at-juno.com (Nancy K.R. Smith)
Date: Tue, 30 Jan 1996 11:27:18 PST
Subject: SED For Philips STEM

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To: microscopy-at-Sparc5.Microscopy.Com

Dear all:
I have attempted to respond directly to Alwyn Eades at 2 different email
addresses listed in his 1-29 posting & get an error message of "illegal
host/domain". So I apologize for posting this to the list.

I know someone who knows someone who bought a Philips SEM at our
university's auction. I think he has given up on getting it set up. So he
might be willing to sell parts from it. I don't remember the model no. It
was one of only about 3 that were made with a motor-driven stage, about 1978
vintage. I have no idea any parts might be useable on your system. Contact
me if you want to pursue it.

Nancy Smith
nkrsmith-at-juno.com cc:smithn-at-uthscsa.edu
210-567-3861 Fax 210-567-3803




From: DLIETZ-at-trentu.ca
Date: Tue, 30 Jan 1996 15:01:47 -0400 (EDT)
Subject: subscribe

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From: DLIETZ-at-trentu.ca
Date: Tue, 30 Jan 1996 15:05:51 -0400 (EDT)
Subject: subscribe

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I would like to subscribe again to see what's going on in the field of
microscopy.





From: MicroToday-at-aol.com
Date: Tue, 30 Jan 1996 15:21:35 -0500
Subject: Database Software

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Hi Theresa -
Regarding your inquiry, I would MOST strongly recommend "Q&A" for two
reasons:
1) It is a true piece of cake to identify fields as "keyword" fields, then
in each you can have an unlimited number of variables to search on - and
build, if you want separate records, mailings, etc. The variables can be in
any form. I happen to use codes (like "A") to indicate a particular
category, but you can use anything - including full names.
2) Unlike the current highly regarded database systems (i.e. Foxpro, etc.)
Q&A is a snap to learn. A half dozen hours of study, and you are up and
operating.
And, in addition, it is not very expensive.
Good luck and regards,
Don Grimes, Microscopy Today





From: Paul Webster :      Paul.Webster-at-quickmail.yale.edu
Date: 30 Jan 1996 18:18:55 -0500
Subject: Re: Workshops

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Message-Id: {n1389096353.87437-at-QuickMail.Yale.edu}

Phil Rutledge writes
"I was wondering if anyone knows of any upcoming workshops in molecular
biology using molecular probes for confocal and electron microscopy. I
prefer a workshop where "hands on" techniques are emphasized."

The 1996 EMBO course which is being held in Prague will have a session on *in
situ* hybridization (as well as cryosectioning, immunolabeling and stereology).
These courses are always "hands on" but the competition to get accepted on them
is tough.

For more details contact:
Dr. Ivan Raska
Institute of Experimental Medicine AS CR
Albertov 4
CZ-128 00 Praha 2
Czechoslovakia
E-mail: raska-at-site.cas.cz
Phone: +42-2-2491 0315
Fax: +42-2-294590





From: A. Kent Christensen :      akc-at-umich.edu
Date: Tue, 30 Jan 1996 12:06:30 -0500 (EST)
Subject: Re: RE-Cleaning Oil Imms Lens

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The original question referred to a situation where it was indeed
necessary to clean cedarwood oil off an immersion lens. However, in
routine use of an oil immersion lens, it has been my understanding over
the years that it is best not to clean the lens. We use commercial
non-drying immersion oil, and merely wipe the excess oil off the lens with
a tissue after use.

A. Kent Christensen, University of Michigan, {akc-at-umich.edu}

-------------------------------

On Tue, 30 Jan 1996, Tobias Baskin wrote:

} Greetings,
} Will Bigelow wrote:
}
} } [snip]... In any event, I think you
} } should be particularly careful not to use acetone or alcohol, because they
} } will soften and dissolve the cement that is usually used to hold the lenses
} } in place. Best of all, check with the manufacturer of the lens.
}
} What is the reason for xylene being less likely to dissolve cement
} than acetone or ethanol? I would have thought that it depended on the
} cement and that generalizations would be difficult. Thanks for any insight
} on this.
}
} Tobias Baskin
}
} - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
} ___ ____ ^ ____ _____ Tobias I. Baskin
} / \ / / \ / \ / University of Missouri
} / | / / \ / / Biological Sciences
} /___ / /__ /_____\ / /__ 109 Tucker Hall
} / / / \ ( / Columbia, MO 65211 USA
} / / / \ \ / voice: 573-882-0173
} / /____ / \ \____/ /_____ fax: 573-882-0123
}
}
}




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Tue, 30 Jan 1996 15:34:57 GMT
Subject: Flat Scanners

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Back a few weeks to our discussion of flat bed scanners. It was noted that
negatives need to be 1-2 stops overexposed for the transparency adapters to
work properly.

UMAX has come out with a new version of there software that operates
under Windows '95 that allows one to adjust the lamp intensity. I have not
tried it. However one assumes that this would then enable one to compensate
for light or dark materials that fall outside the range of the automatic
settings
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 30 Jan 1996 10:45:48 -0500
Subject: Scann. Microsc. Int. Meet.

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Message-Id: {n1389123256.18761-at-msmail.tmc.tulane.edu}

Scanning Microscopy International
Post Office Box 66507, Chicago (A.M.F. O'Hare), IL 60666-0507,
U.S.A.
Telephone: (708) 529-6677 / FAX: (708) 980-6698
E.mail: 73211.647-at-compuserve.com

Scanning Microscopy, Cells and Materials, and Food Structure 1996
meetings will be from May 11 to 16 at the Hyatt Regency Hotel,
Bethesda, MD (suburb of Washington, D.C.). Programs on the follow-
ing topics are already being planned (*fliers available, list
below): *Scanning Probe Microscopies (including STM, AFM, etc.) and Related
Techniques for the Biological and Materials Sciences (program already has
over 50
papers); Physical sciences programs in: *Fundamental Physics in
Microscopy and Microanalysis (program of 20+ papers), *Pattern
Formation and Nanoscaled Structures in Thin Film Formation (program
of 25+ papers), *Scanning Microscopy and Semiconductors: Metrology
and Diagnostics (Program of 25+ papers); Biological programs in:
Microanalysis and Imaging, *Immunolabelling, *Radiation Effects,
Apoptosis, *Dentistry, Corrosion Casting, *Inner Ear, *Bone
Biology, *Stones and Crystals, etc.); *Cells and Materials
(covering: Skeletal Tissue / Biomaterials; Foreign Body Reactions;
Biointerfacial Reactions at Biomaterials Surfaces; Innovative Drug
Delivery Systems; Blood Related Biomaterials; and Dental
Biomaterials); and Food Structure related topics. For
participation and contribution, please contact Om Johari at address above.

Scanning Microscopy International is also sponsoring another
international meeting: 15th Pfefferkorn Conference on Electron
Image and Signal Processing (immediately after the Bethesda
Meeting) from May 18-22, 1996 at Silver Bay, New York (80 miles
north of Albany, New York). The organizers are: Drs. Peter W.
Hawkes (CNRS, Toulouse, France; FAX: 33-62-257999; E.mail:
hawkes-at-cict.fr; as its chief organizer), W. Owen Saxton (Univ.
Cambridge, U.K.) and Joachim Frank (NY State Dept. Health, Albany,
NY). Nearly 45 invited contributions are planned; a flier is
available on request. Interested contributors should contact one
of the organizers.




From: Rick Markgraf :      rlmarkgraf-at-ucdavis.edu
Date: Tue, 30 Jan 1996 10:54:30 -0800
Subject: Cleaning Oil Imms Lens

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I agree that xylene and alcohol are probably equal. An old Leitz
Ortholux manual warned against using "spirits" to clean lenses. Modern
cements are unlikely to be affected by either, but I still choose to use
them sparingly. I always use 6" cotton-tipped applicator sticks and, after
dipping them in xylene, press the bud against a tissue until it is just
damp. It makes more sense than soaking the lens and cleans just as well. I
also follow up with a dry bud when cleaning oil from a 40X objective. Then,
I use the same method with Windex or other commercial glass cleaner to
remove all traces and clean the lens thoroughly.
Cotton tipped applicators have an enormous advantage over lens paper.
They work better on small, concave, or recessed lenses, they aren't touched
by your fingers, and so never transfer skin oils, and they prevent contact
between your skin and any toxic solvents (such as xylene) you may use.
I never use acetone on an objective. Some (mostly American Optical)
have a painted "mask" around the lens, and acetone can dissolve the mask and
deposit the paint onto the lens.

} Will Bigelow wrote:
}
} } [snip]... In any event, I think you
} } should be particularly careful not to use acetone or alcohol, because they
} } will soften and dissolve the cement that is usually used to hold the lenses
} } in place. Best of all, check with the manufacturer of the lens.
}
} What is the reason for xylene being less likely to dissolve cement
} than acetone or ethanol? I would have thought that it depended on the
} cement and that generalizations would be difficult. Thanks for any insight
} on this.
}
} Tobias Baskin
}
} - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
} ___ ____ ^ ____ _____ Tobias I. Baskin
} / \ / / \ / \ / University of Missouri
} / | / / \ / / Biological Sciences
} /___ / /__ /_____\ / /__ 109 Tucker Hall
} / / / \ ( / Columbia, MO 65211 USA
} / / / \ \ / voice: 573-882-0173
} / /____ / \ \____/ /_____ fax: 573-882-0123
}
}
}
}
Richard L. Markgraf
Microscope Services
University of California, Davis
Ph. (916)752-3477 Fax (916)752-6363
rlmarkgraf-at-ucdavis.edu





From: ScottE57-at-aol.com
Date: Tue, 30 Jan 1996 20:47:17 -0500
Subject: Re: database for microscopy

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Advanced Imaging Concepts Image Central Version 2.0 windows image database is
very applicapble here. Please contact us for further information.

Scott E. Berman
Advanced Imaging Concepts, Inc.
Phone (908) 274-1877
Fax (908) 274-1974
e-mail Scott E57-at-aol.com






From: BAKERK 905-822-3520(265) :      BakerK-at-aa.wl.com
Date: Tue, 30 Jan 1996 14:30:45 -0400 (EDT)
Subject: Fiducial Points

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Mr-Received: by mta SPVX01.MUAS; Relayed; Tue, 30 Jan 1996 14:30:45 -0400
Mr-Received: by mta SPVX01; Relayed; Tue, 30 Jan 1996 14:30:46 -0400
Mr-Received: by mta SRVR01; Relayed; Tue, 30 Jan 1996 14:32:02 -0400
Disclose-Recipients: prohibited



Does anyone have any suggestions for placing fiducial points in tissues that
are intended for embeddment, serial sectioning and immunolabeling? We are
hoping to reconstruct serial sections as image stacks, ultimately for the
purpose of
measurement and visualization.

Some have suggested surface cuts, embeddment of threads, or even the inclusion
of some kind of stainable implant.

Thank You in advance for any and all comments.

KWB
905-822-3520
BAKERK-at-AA.WL.COM






From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 30 Jan 1996 13:50:55 -0500 (EST)
Subject: Re: database for microscopy

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Message-ID: {n1389083118.76446-at-mse.engin.umich.edu}

} Does anyone know of database software that I could use to (1) input the
} form our lab uses for each sample that comes in (2) search the records by
} chosen field of information like "cell line" or "users name" for example
} and (3) build a new file with the records from that search? This would
} enable me to answer questions like "what were all the experiments done on
} x cell line from y time frame?" or place all the records from one user
} in one file that I could then print or give to him/her. I would also
} like to make a rapid index with just the identification number, date,
} user and cell or tissue type, as a rapid frame of reference.
}
} Is there software to do this? Any recommendations or experiences?
}
Dear Theresa,
Any relational database program will do everything you ask. I have
experience with dBASE, which works well, but is not free. All you have to
do in a relational database program is to define fields with the requisite
info, e.g., for the field "C_LINE" put in the cell line, etc., then use the
search utilities to do boolian searches. The index file can be embedded in
the main data file; you only need to print or list those fields in which you
have an interest. dBASE can print these on forms which you can design to
meet your own needs. As I said, almost any database program can do these
things, so the one to choose is the one you can get most cheaply and/or
which you can use best. Good luck.
Yours,
Bill Tivol




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 30 Jan 1996 13:59:07 -0400
Subject: More on Immers Oils

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Message-ID: {n1389111783.51258-at-mse.engin.umich.edu}

Subject: Time: 1:27 PM
OFFICE MEMO More on Immers Oils Date: 1/30/96

Wow, did I open a can of worms when I mentioned the article on immersion
oils by J. J.Cargille. Actually, the article was written in 1964, and is
possibly quite a bit out of date by now. Basically it covers the following
points:
1. describes the function of immersion oil in increasing the
numerical aperture of a lens by increasing the refractive index between the
objective and condenser.
2. Discusses the problems involved in formulating immersion oils, most
of which are of no direct concern to users of them.
4. Notes that a variation of 1 C in temperature can change the
refractive index of an oil by approx. 0.0004, so be sure to work at the temp
given on the bottle
5. Synthetic oils can be formulated to have better properties than most
natural oils;
- freedom from color which may degrade performance
- lower volatility and more resistant to oxidation and
photo-decomposition, and so less likely to thicken and to form a gummy
deposit on the lens. Wont thicken over time and change ref. index.
- lower acidity and so less likely to damage your instrument,
He also mentions that although Red Cedar oil has been one of the most
commonly used oils: it has poor adsorption characteristics because it may be
discolored; it contains volatiles and will leave gummy films on lenses unless
cleaned off promptly, and it may thicken over time due to evaporation and
change refractive index; it usually has a relatively high acidity, and so may
be prone to damage the objective with prolonged use.
He does not explain why it is common practice to use xylene to clean
immersion oil off lenses, except to say that it doesn't damage the lens
cement. I don't know whether this is the solvent recommended by all
manufacturers of microscopes. In these times when such a wide variety of
cements are available there may be some exceptions. However, I did check the
instructions for a Nikon microscope we purchased only a couple of years ago,
and xylene was recommended there.
The rule I facetiously give to my students concerning the approach to
using instruments is, "After all the controls are bent and everything is
completely fouled up, read the instruction manual!"
The present address of the Cargille Company is: R. P Cargille
Laboratories, 55 Commerce Road, Cedar Grove, NJ, 07009 (Ph. 201-239-6633;
Fx: 201-239-6096) if you want more up-to-date info on immersion oils.
Sorry to get everyone so stirred up:
Wil Bigelow (bigelow-at-umich.edu)





From: mrg1995-at-htp.net
Date: Tue, 30 Jan 1996 23:00:16 -0600
Subject: Subscribe Microscopy@MSA.Microscopy.Com

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Subscribe Microscopy-at-MSA.Microscopy.Com
MRG Associates, Inc.
[Worldwide Headquarters]
755 Waverly Avenue
Holtsville, New York, USA 11742
1-800-606-8869 (Voice within USA)
1-516-447-1041 (Voice)
1-516-447-1042 (FAX)
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O





From: Tina Carvalho :      tina-at-halia.pbrc.Hawaii.Edu
Date: Tue, 30 Jan 1996 09:17:15 -1000 (HST)
Subject: Re: Fed audit at Univ Hawaii

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Quite a few people have asked me for details of the federal audit at the
University of Hawaii that deemed our billing practices fraud.
Below is a brief summary that I sent to one interested person a couple
of months ago--

} We apparently had two things going on here that annoyed the Feds. The
} first, and the one that should be of the most concern to other
} facilities that operate on a recharge basis, is that a Federal audit
} alleged that we were making charges to Federal grants in advance of
} receipt of services. We had been selling "shares" of instrument time,
} allowing users to prepay for 80 hour blocks of time in advance. More
} casual users who paid for time by the hour paid roughly twice as much
} per hour as those who bought blocks of time. This was an incentive to
} get people to commit to instrument usage, allowed us to collect monies
} with which to pay our service contracts (which must be paid in advance),
} expedited paperwork, and allowed users to encumber funds in a timely
} manner. Everything went along fine for about 6 years, but then the Feds
} decided that this constituted fraud. And they weren't very nice about
} it, either. (As an aside, the auditors admitted that our bookkeeping
} was exemplary and that the shares bit was a great and efficient idea and
} should be allowed, but it isn't, so they slapped us anyway.)
}
} The second problem is related, and was actually the more serious on in
} the Feds eyes, even though it involved less money. Apparently our
} umbrella research unit transferred some funds to us as sort of a cross
} between a retainer and a gift, to be used by one of the other research
} labs if needed, but as a gift if not used. Unfortunately, they had us
} write an invoice for it that said it was for use of the EMs, which were
} never used. This was "clearly fraud". I guess if those monies had been
} labelled as for core support or as a gift or whatever, it would have
} been (almost) OK. The net result of these two practices was that about
$20,000 was taken from us, presumably to be returned to the Federal grants
} from which it came. Plus interest! In actuality, the $$ sits in some
} account somewhere where no one knows what to do with it, and the matter
} has been shelved. Meanwhile, we have allowed, over the last four years,
} the users involved to continue to "work off" the money they had put into
} the facility, at our loss. And so, with Federal and Hawaii state budget
} cuts, we're in deep kim chee. The $$ that was taken from us would have
} paid all our service contracts this year, for instance, and we are now
} broke...! And we can't see breaking even for years to come.
} I can see the Fed's points, they could see ours, and everybody lost on
} this one. Don't let it happen to you!

Other things that must be considered include the federal granting
agencies rules that you may not charge federal grants any more than the
lowest rate you charge anyone else. With monies for any given
project often coming from several sources, we quit trying to have
differential charges altogether, so now we charge outside, commercial
projects the same as internal U.H. projects. (Fortunately, there are no
provate EM companies against whom we can't compete, so we don't have to
worry about the politics of taking on any outside projects.) But then
there could be a question about the taxpayers footing part of the bill
for commercial users...

We had a site visit from a federal agency that supports roughly a third
of our facility base costs (my salary included) two weeks ago. They
seriously questioned why we didn't give preferential rates for users of
the facility that were also funded by their agency. I'm confused,
because if we have lower rates for their users, then other federal
grantees would not have the lowest rates, and if we lowered them, then
agency #1's rates would not be preferential, and so it would go until it
was all free! BUT THEN one of the site visitors wanted to know what we
would do if a faculty member had no money but needed to get preliminary data
for a proposal, could we provide any services for free? And while one
member was asking me that, another across the room was telling the
facility manager that we CAN'T do any work for anyone for free because
they were supporting us... They obviously don't have a unified plan.

The basic problem still remains that we have to pay our service
contracts IN ADVANCE, but we have no reserve pool of money from which
to do so (and some facilities are prohibited from accumulating a pool
of money, anyway). And you can't collect money in advance of services
rendered. Other recharge facilities here are in the same boat. It
takes a lot of money for, say, DNA sequencing or protein synthesis, or
whatever. The facility here would get the $$ up front to pay for
gearing up to do it. That is now illegal. So they have to do it first
(how?) and then charge the customer. And not make a profit to have the
reserves to gear up for the next task...

Anyone have any words of wisdom?



Aloha nui,
Tina
}
} *****************************************
} Tina (Weatherby) Carvalho *
} Biological Electron Microscope Facility *
} University of Hawaii *
} (808) 956-6251 *
} tina-at-ahi.pbrc.hawaii.edu *
} http://www.pbrc.hawaii.edu/bemf/ *
} *****************************************
p.s., Yesterday was gorgeous, about 83 degrees F, but today we expect
rain, just to keep the mountains green.






From: Pogany Lajos :      pogany-at-power.szfki.kfki.hu
Date: Wed, 31 Jan 1996 05:58:23 +0100
Subject: CD ROM for archieving

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Errors-To: borbas-at-sunserv.kfki.hu

Dear subscribers,

Is anyone able to assist me in finding a CD ROM writer for image archieving
in the price range of about 1000 $?
Would be possibile to send me addresses where are those available?

Thanks for Your help

LAjos Pogany
pogany-at-power.szfki.kfki.hu






From: Hasse Ekwall :      Hans.Ekwall-at-ah.slu.se
Date: Wed, 31 Jan 1996 08:51:47 +0100
Subject: subscribe

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Subscribe Microscopy-at-MSA.Microscopy.Com


SWEDISH UNIVERSITY AGRICULTURAL SCIENCES
Hans Ekwall
Dept. Anatomy & Histology
Box 7011, S-750 07 Uppsala, SWEDEN

E-mail Hans.Ekwall-at-ah.slu.se
Voice: +46 18 672141 Telefax: +46 18 672852






From: s.griffiths-at-ucl.ac.uk (Stephen Griffiths)
Date: Wed, 31 Jan 1996 09:09:28 +0000
Subject: Re: LM: Objective Lens cleaning

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Message-Id: {199601310905.DAA12836-at-Sparc5.Microscopy.Com}
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Going back a few years, as I do unfortunately, I remember the Microscope
Service Engineer telling me never to use Xylene as it dissolved the mounting
medium of the lens. He always used Ether.

The problem was he used to chuck the Ether soaked lens paper into the metal
wastebin. He also used to smoke. And smoking wasn't banned in the Lab in
those days. (I said I went back a few years). When he also threw his
cigarette end in the bin, the result was exciting, (excessively so) to say
the least.

I still use Xylene. Nothing fell out yet. :)

Regards
Stephen

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
{} Stephen Griffiths {} e-mail s.griffiths-at-ucl.ac.uk {}
{} Visual Science Department {} {}
{} Institute of Ophthalmology {} Tel: 0171 608 6914 {}
{} London. EC1V 9EL. UK. {} Fax: 0171 608 6850 {}
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}





From: Stefan.Gunnarsson-at-devbiol.uu.se (Stefan Gunnarsson)
Date: Wed, 31 Jan 1996 08:48:46 +0100
Subject: Re: Cleaning Oil Imms Lens

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It seems that there are quite a lot of different methods of cleaning
lenses. My favourite method, for what it is worth, is to use either a
proprietary mixture form Leica here in Sweden (isopropanol, some detergent
and dist. water, I think) or abs. ethanol (both are OK from the
manufacturer). After wiping the excess oil off around the lens (never on
the lens), I put a drop of this on the lens, let it sit for a while, then
blow it off in one go with clean compressed CO2. I repeat as many times as
necessary. In this way I never need to touch the lens surface which
according to many authorities (e.g. Shinya Inou=E9 in his book "Video
Microscopy") may be detrimental as the surface coating of the lens is very
soft and easily scratched or abrased even by "soft" cotton or lens cleaning
paper.
I prefer not to use solvents like xylene due to their potential health
risks which I think should be considered in this context.
Finally, it would be nice if reps. from each of the large microscope
manufacturers could give the final words in this matter (what is best for
the lenses, what can the cement take, what if there is other stuff than oil
to clean (e.g. Moviol or other mountants), what is the best way to clean
non-oil immersion lenses that have been messed up with oil or other
things).

Stefan



............................................................................=
..

Stefan Gunnarsson
Microscopy Unit,Dept. of Animal Development and Genetics

Uppsala University
Norbyv. 18A, S-75236 UPPSALA, Sweden
e-mail Stefan.Gunnarsson-at-devbiol.uu.se








From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 31 Jan 96 09:14:07 EST
Subject: database

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For Macintosh users,I collate and segregate with Claris Works database. I do
not know if this is a DOS program.
Kate




From: DLIETZ-at-trentu.ca
Date: Wed, 31 Jan 1996 10:06:59 -0400 (EDT)
Subject: computer archiving LM and SEM

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I have recently returned to Trent U and we have started to look at a
computer archiving system which we would like to use in teaching. We have
a small computer (MACS) lab which we are expanding. I am interested in
finding out what systems other labs are using to grab images. Our aim is
to grab images from an SEM, a CCD hooked up to a LM or Video camera and be
able to enhance the images and add script and then download them through
our LAND to the teaching lab. We also wish to do this all in colour. If
you can give me any leads please reply.




From: Doug Keene :      DRK-at-SHCC.ORG
Date: Wed, 31 Jan 1996 09:53:50 -0800 (PST)
Subject: Re: Cleaning Oil Imms Lens

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We have been using Q-tip brand cotton swabs dipped in chloroform to clean our
Zeiss objectives. The method works extremely well, and requires a minimum of
scrubbing. Using this method over the past 7 years has resulted in no
problems. I would caution that other cotton swabs may
scratch the lenses. Doug Keene, Shriners Hospital Research Unit




From: DonPfield-at-aol.com
Date: Wed, 31 Jan 1996 15:56:00 -0500
Subject: Fisons/Kevex upgrade

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I have a Fisons/Kevex 770 XRF system and would like to upgrade the computer
systerm to a Windows based software system. Does anyone know of other
sources of upgrade hardware/software other than the manufacturer?
Thanks

DonP




From: Delilah Irving :      dirving-at-aggie.pw.usda.gov
Date: Wed, 31 Jan 1996 12:30:50 -0800 (PST)
Subject: Re: Cleaning Oil Imms Lens

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I use Kodak lens cleaner on a cotton swab and blot with a clean cotton
swab (Q-tip will do) for both oil immersion and non-oil immersion lenses.
It's certainly of less of a health threat than xylene or chloroform and
since it comes in a squeeze bottle is not likely to become contaminated by
dipping cotton swabs into it.

De Irving, USDA, Albany, CA





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Wed, 31 Jan 1996 17:16:30 -0600
Subject: Stain problem

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I am having a recurring problem with either my grid stain, or water in my
knife boat, or wash water. We stain epoxy sections on copper grids with the
usual uranyl acetate in 50% methanol followed by Reynold's lead citrate.
These are washed in ultra pure water boiled to remove carbonate. We have
done this routine stain procedure successfully for years and have not
changed our materials or procedure in any way. However recently our sections
have been plagued by almost perfectly hexagonal shaped deposits of electron
lucent to opaque "stuff". These deposits aren't embedded within the epoxy as
they ripple and burble under a concentrated beam. Microprobe (EDXEA) of the
deposits gives a solitary lead peak (no uranium peak), but it is unclear
whether the material is an inappropriate lead deposit or another material,
that may have come from the boat or wash water, that is binding the lead
stain. Between the hexagons, we see that the embedded tissue stains well. I
would appreciate any suggestions as to what this might be and how to stop it.


Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: W.Jablonski-at-csl.utas.edu.au (Wis Jablonski)
Date: Thu, 01 Feb 1996 10:19:35 +1000
Subject: Stain problem

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Dear All,
Could you please, help me to locate E-mail and other contact items of Prof.
William A. Miller, Professor of Oral Biology , some times ago at State
University of New York at Buffalo. Please contact me directly on my Email,
W.Jablonski-at-csl.utas.edu.au
Regards to all, and happy New Year 1996,
Wis Jablonski, OiC, EM/X-ray Microanalysis, CSL, Uni of Tasmania





From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Thu, 1 Feb 1996 10:04:08 +1100
Subject: cleaning LM lenses

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I was recommended to clean any gunked up lens with a knob of polystyrene
foam - no chemicals, just the dry foam rubbed in a circular motion onto the
lens. Over the years none of my Zeiss lenses has suffered any damage from
this.


Diana van Driel
Dept Ophthalmology
Sydney University 2006
NSW, AUSTRALIA






From: MicroToday-at-aol.com
Date: Wed, 31 Jan 1996 18:41:04 -0500
Subject: More on database S/W

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Considering the number of comments and questions I received concerning my
recent recommendation of Q&A database software, kindly allow this response to
the full list:
The name of the software is "Q&A" and it is made by Symantec. Going back
some 5-6 years, it was highly recommended by a number of the computer
magazines. Pricing used to be listed by the software vendors in the back of
these computer magazines, and I expect that they still do - or you might
call Symantec direct (408)253-9600.
To further explain its advantage, we all know that all database software has
a common set of fields. And the normal application is to put only one
variable in each field. For example, one might have a "last name" field - in
which goes only last names. Theresa's question was to S/W that can
conviently handle a considerable number of variables for each record.
In Q&A during setup, you can designate a field as a keyword field. Then in
that field you can have hundreds of variables - each separated by a
semicolon. You can then search (include or eliminate) just as if your
variables were in the dedicated fields. And you can have more than one
keyword field - and search (include or eliminate) on variables from BOTH
fields.
For example, one of my keyword fields is "interests". I enter whether the
person is a "user" or a manufacturer/supplier, his/her primary field(s) of
microscopy interest (biology, material or earth science), and then his/her
specific interests (electron, light, confocal, AFM, etc., etc.). In this one
field I happen to have a total of 14 variables. If the person is a user I
enter an "A", or if he/she is a manufacturer I enter a "B", if he/she is
interested in electron and confocal microscopy, I enter an "C" and a "D",
etc.- each code # followed by a semicolon. I could, of course, enter real
text rather than the code. Say I want to find users only with an interest
in biology and light microscopy. A piece of cake! And to extend the search
to include only those who work in an individual company in a specific town is
so, so easy.
I do understand that other database software allows these kind of searches
but all I have looked at, and I have looked at most, makes it somewhat to
very difficult.
I hope that a number of you find these comments of interest.
Regards,
Don Grimes, Microscopy Today




From: Tina Carvalho :      tina-at-halia.pbrc.Hawaii.Edu
Date: Wed, 31 Jan 1996 10:54:05 -1000 (HST)
Subject: Audit woes

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I would like to thank all of you who have responded to me personally
with sympathy or ideas concerning our audit woes. Actually, we are OK
right now (except for always operating in the red, which we are getting
away with for now). My message was primarily to stimulate discussion on
the subject of recharge problems, and as a warning to other facilities
who may be subject to such scrutiny in the future. Rearranging our
billing practices was not so awful; losing a chunk of money and being
psychologically dragged over the coals was.

In my opinion, each facility that is not totally subsidized by their
institution needs to take a good look at their sources of funds, and at
what their outlay is. We basically have to justify every nickle of our
hourly charges for instrument use, for example. We have built into that
charge some percentage of the costs of consumables, technician salary
for maintenance, cost of other down time, whatever. We do not add in a
portion of the service contracts because they are (partially) supported
by other sources. We should, however, add in that portion NOT covered
by other sources. But the calculations have gotten so cumbersome as it
is, that we have not. Plus there recently came up the question of use
by faculty partially supported by the same grant as supports part of the
service contract, and should that be prorated? It gets unbelievably
complex really quickly. So I'm just thorwing this out there to (make
your lives more miserable, and) warn others of the potential
budgetary/political problems that may come out of the blue. I expect
each situation to be different. I am interested in finding ways to go
back to the "lump sum" situation for our regular users. For instance,
perhaps they can pay outright for a percentage of the service
contracts, and then not be charged by the hour. It probably all depends on
the current person sitting in the fiscal or contract office, or what they
are wearing, or whatever. I'm interested in what works for other
facilities.

Our rainstorm blew through, and today it's 83 degrees, clear and sunny.
And I'm stuck in this EM lab...

*****************************************
Tina (Weatherby) Carvalho *
Biological Electron Microscope Facility *
University of Hawaii *
(808) 956-6251 *
tina-at-ahi.pbrc.hawaii.edu *
http://www.pbrc.hawaii.edu/bemf/ *
*****************************************





From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Wed, 31 Jan 1996 23:11:44 -0700
Subject: Re: Audit woes

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Thanks, Tina, for sharing your woes with the readers of the microscopy list.=
As was discussed at the Technologist's Forum at MSA last year, these=
issues affect all EM service labs that deal with federally funded research=
projects. The answers are not always easy, but there are some basic=
guidelines that we use at Colorado State University that could be applied=
at other universities.

** some deleted **

} In my opinion, each facility that is not totally subsidized by their=20
} institution needs to take a good look at their sources of funds, and at=20
} what their outlay is. We basically have to justify every nickle of our=20

This is what we have found. Financial support of EM labs from central=
sources, like college deans and vice presidents for research is essential. =
When we polled about 2 dozen EM labs at universities comparable to ours a=
few years ago, the clear message was that to not have central support was=
"the kiss of death".

} hourly charges for instrument use, for example. We have built into that=20
} charge some percentage of the costs of consumables, technician salary=20
} for maintenance, cost of other down time, whatever. We do not add in a=20

We have had to do rigorous cost accounting to find out as accurately as=
possible what the hourly cost of operating the instruments is. We know=
what the fixed costs for each instrument are. They are service contracts,=
gases, liquid nitrogen, an estimated number of hours for routine=
maintenance, etc. Those expenses are divided by the expected usage, in=
hours, to come up with an estimated hourly charge. Our best estimate for=
our instruments is $45/hr in actual cost to run the instruments.

We have gone through several mechanisms over the last 5-10 years to get=
financial support from our central administration. What happens this year=
and next is that several central sources have paid enough dollars to cover=
some of the fixed costs, specifically service contracts, which allows us to=
bill every user at CSU the same reduced rate of $17/hr. There are three=
important points to remember; 1) no dollars from federal grants are used=
for the up front payments, so there is no pre-payment for services, 2) all=
CSU users are billed the same amount for the entire fiscal year, and 3) the=
hourly charge reflects real expenses.

This entire process is a collosal pain, but it is essential, if we want to=
stay in compliance with federal regulations. We think the effort will=
serve us well, should we be audited. We have been working closely with the=
financial officer of our college and the university's controller to be as=
sure as is possible that our policies will pass their scrutiny, as well as=
that of auditors.

These policies aren't used only in the EM Center, but also at other centers=
around the university, and will be used by more. So what happens at other=
labs at your university might be useful to you.

} portion of the service contracts because they are (partially) supported=20
} by other sources. We should, however, add in that portion NOT covered=20
} by other sources. But the calculations have gotten so cumbersome as it=20
} is, that we have not. Plus there recently came up the question of use=20
} by faculty partially supported by the same grant as supports part of the=20
} service contract, and should that be prorated? It gets unbelievably=20

A multi-tiered fee schedule invites such headaches that we won't even=
consider it. The bookkeeping is so cumbersome and convoluted that we think=
it invites trouble.

Our philosophy is that we will bill for real expenses fairly, so no one gets=
gouged. We had been subsidizing the campus for a long time, and, once we=
decided to clean up the books, we made everyone acccountable.

We are trying to meet our expenses as closely as possible, without making=
money. If we end the year with a deficit, we can legitimately charge that=
against next year's budget and recoup it through adjusted fees next fiscal =
year.

} complex really quickly. So I'm just thorwing this out there to (make=20
} your lives more miserable, and) warn others of the potential=20
} budgetary/political problems that may come out of the blue. I expect=20
} each situation to be different. I am interested in finding ways to go=20
} back to the "lump sum" situation for our regular users. For instance,=20
} perhaps they can pay outright for a percentage of the service=20
} contracts, and then not be charged by the hour. It probably all depends on=
=20

This is the kind of arrangement that we think is sure to get us in trouble,=
so we won't consider it. Once the true costs of running the facility are=
known by the users, they are much more likely to go along with the charges.=
Everyone would like to get the services for free, but it's hard to argue=
with facts like real costs.

So far, this approach is working very well. And, since we're still looking=
for better ways to recoup expenses, I'm interested in any other procedures=
that work.

} the current person sitting in the fiscal or contract office, or what they=
=20
} are wearing, or whatever. I'm interested in what works for other=20
} facilities.

John
chandler-at-lamar.ColoState.EDU






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Wed, 31 Jan 1996 23:29:01 -0800
Subject: Re: Fisons/Kevex upgrade

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} I have a Fisons/Kevex 770 XRF system and would like to upgrade the computer
} systerm to a Windows based software system. Does anyone know of other
} sources of upgrade hardware/software other than the manufacturer?
} Thanks

Dear Don,
The company IXRF have contacted old Kevex EDS users with a system to run
EDS software on a Pentium computer, using the power supplies and pulse
processor of your existing Kevex. With a name like IXFR, I suspect they do
XRF systems, too.
Contact:
Kenny Witherspoon
IXRF Systems, Inc.
15715 Brookford Drive
Houston, TX 7705
tel: 713-286-6485, fax: 713-286-2660

They will probably be at MAS/MSA/MSC, too.
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Wed, 31 Jan 1996 23:20:51 -0800
Subject: Re: database for microscopy

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} Does anyone know of database software that I could use to (1) input the
} form our lab uses for each sample that comes in (2) search the records by
} chosen field of information like "cell line" or "users name" for example
} and (3) build a new file with the records from that search?
Dear Theresa,
I have been using Microsoft Access for keeping track of my billing in my
lab for about three years now. It is a relational database and you can
design the particular "queries" to relate any data to any other. I do
Christmas cards at home, keep track of SEM hours, photos, different
customers and rates at work. I know it will do a lot more than I use, and I
find it easy to change things if I need to.

I suspect any good database would be sufficient for your use, it is just a
matter of choosing one. The learning curve is a bit slow at first, but you
soon learn.

Hope this helps,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: RMacKay :      RMACKAY-at-AC.DAL.CA
Date: Thu, 1 Feb 1996 10:49:23 +0000
Subject: EMPA Software

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Message-Id: {199602011357.JAA16914-at-Snoopy.UCIS.Dal.Ca}
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To those in probe land,

Occasionally I am asked to identify an unknown mineral based on
microprobe data. Does any one know of an inexpensive software
program that would run on a PC ?

Best Regards,

Bob MacKay
Robert MacKay
Department of Earth Sciences
Dalhousie University
Halifax, Nova Scotia, Canada
B3H 3J5
Tel: 902 494-7087
e-mail rmackay-at-ac.dal.ca




From: Leo Marin :      leo-at-spine.med.utoronto.ca
Date: Thu, 1 Feb 1996 09:58:12 -0500 (EST)
Subject: Fiducial points

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I have been thinking about a similar problem with respect to serial
sectioning of the hippocampus. Could the use of a microbeam laser to
produce a micron-size hole in the tissue be part of a solution?
Leo Marin
Dept. of Physiology
University of Toronto




From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 01 Feb 1996 11:03:59 -0600
Subject: Re: Stain problem

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At 08:43 AM 2/1/96 -0500, you wrote:
......My conclusion was that the Pb was present as an impurity on the grids,
some batches have it, some don't.
}
*************
That's something I hadn't thought about sinse we routinely wash our grids
(copper mesh) in acetone followed by alcohol. We will try an acetic acid
wash next followed by thorough rinsing in clean water and see what happens.

* * Joiner Cartwright, Jr. * *





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 01 Feb 1996 11:03:56 -0600
Subject: Re: Stain problem

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At 08:37 AM 2/1/96 -0500, you wrote:
.....perhaps the problem appeared after the resin cartridges were exchanged
and hence
} most likely to leach chelating agent.
}
*************

That's a good point. However the water used is from a reverse osmosis system
and shouldn't have that problem. Also up until the recent past, we had
indeed used resin demineralized water without problem. Thanks for your input.

* * Joiner Cartwright, Jr. * *





From: Clint_Haris-RA3813-at-email.sps.mot.com
Date: 1 Feb 96 11:46:35 -0600
Subject: unsubscribe

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From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 01 Feb 1996 11:04:02 -0600
Subject: Re: Stain problem

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At 09:20 AM 2/1/96 EST, you wrote:

.....we thought it was coming from the lead solder in the water
pipes.....you might ask Don Steel at alcan.....

**************
Paul -

I just read Don's letter and am going to try his suggestion to wash grids
better. We have had this problem with both resin demineralized water as well
as water purified by reverse osmosis that is used in tissue culture. The
success of our tissue cultures seems to preclude a significant lead content
in the latter.

I must admit that I feel a bit better hearing that others have come up
against these -at-#%&*-at-#$! hexagons.
* * Joiner Cartwright, Jr. * *





From: Rea, Thomas -TREA
Date: 1996-02-01 11:02
Subject: looking for MS-Access example databases

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------------------------------------------------------------------------------




Can anyone give me a good http or ftp or even a BBS site that has MS-Access
example databases (i.e., Shareware, etc.....).

I have been looking on the WWW and
I have found next to zilch!!!!


Thanks!

Tom Rea
TREA-at-chevron.com





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 01 Feb 1996 15:16:49 -0600
Subject: Re: Stain problem

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At 10:10 AM 2/1/96 -0800, you wrote:

} The hexangonal crystals are probably uranyl acetate, not the lead. Take
} a look at your Uranyl staining procedure. The stain is probably over
} saturated or drying out at some point or possibly too old.
}
} Dan

**********************
Dan -

I'll check that out and see what happens. However EDXEA microprobe of the
contamination yielded a lead peak only.....but, shoot, I'll try anything at
this point.

* * Joiner Cartwright, Jr. * *





From: Donald Lovett :      lovett-at-trenton.edu
Date: Thu, 1 Feb 1996 08:37:58 -0500 (EST)
Subject: Re: Stain problem

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In response to Joiner Cartwright's question about a precipitate that is
forming on his grids when he uses "ultra pure water boiled to remove
carbonate", I have the following suggestion:

I was taught to never use resin-purified water in solutions for EM work.
The rational was that chelating agents in the resin may become
solubilized and later create unwanted precipitate in the specimen. I
only use glass distilled water for all of my EM work. Perhaps the
problem suggested above is due to the water (I assume that "ultra pure"
refers to a resin purification system. If this is the case, perhaps the
problem appeared after the resin cartridges were exchanged (and hence
most likely to leach chelating agent).

__________________________________________________________________________
Donald L. Lovett e-mail: lovett-at-trenton.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
Trenton State College, NJ 08650-4700 fax: (609) 771-2674






From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 1 Feb 1996 16:39:21 -0500 (EST)
Subject: Re: Fiducial points

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} Could the use of a microbeam laser to
} produce a micron-size hole in the tissue be part of a solution?

Dear Leo & all,
Since the object is to produce contrast between the rest of the
section and the fiducial mark, a micron-sized hole would do the job. One
must remember that during the fixing, staining & embedding the hole might
be bent or otherwise distorted, but that information is useful in itself.
An alternative to using a laser is to use a micropipette. We have been
developing micropipettes for HVEM sample holders & have discovered that
they can be pulled to submicron diameters, so they may offer an advantage
over lasers. (Good old low-tech solutions still have merit. :-))
Yours,
Bill Tivol




From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 01 Feb 1996 16:17:02 -0600
Subject: Re: Stain problem

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Thanks, Chuck. That sounds plausable. We'll give it a try.

Joiner
************************************

At 02:52 PM 2/1/96 -0600, you wrote:
} Joiner,
} I posted a message to Microscopy and never saw it come over the
} newsgroup. So anyway....
} We have had the same problem. If we use fresh sodium citrate in
} the preparation of the lead citrate, it goes away. Information, from ?
} somewhere, indicated that sodium citrate can degrade over time. It isn't
} easy buying small quantities of the stuff so we buy 500 grams and aliquot
} out the powder in 15 - to 20 gram portions in scintillation vials and keep
} it out of the light. When we use a vial, we discard any leftover. If two
} vials/two batches of Pbcitrate show problems, we discard any remaining
} vials and order a new batch of sodium citrate.
} We may be fooling ourselves and the leftover sodium citrate is an
} adequate sacrifice to appease the quirky gods of EM. Either way, it works
} for us.
}
} Chuck
}
}
}
} Charles J. Butterick (Chuck)
} Electron Microscopy Center
} Department of Cell Biology
} and Biochemistry
} Texas Tech University Health
} Sciences Center
} 3601 4th Street
} Lubbock, Texas 79430
}
} vox (806) 743-1633
} fax (806) 743-1219
} email emccjb-at-ttuhsc.edu or
} chuck-at-micron1.lubb.ttuhsc.edu
}
}
}
}





From: emccjb-at-ttuhsc.edu (Charles J. Butterick)
Date: Thu, 1 Feb 1996 09:01:16 -0600
Subject: RE: Stain problem

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Joiner Cartwright said,
"I am having a recurring problem with either my grid stain, or water in my
knife boat, or wash water. We stain epoxy sections on copper grids with the
usual uranyl acetate in 50% methanol followed by Reynold's lead citrate.
These are washed in ultra pure water boiled to remove carbonate. We have
done this routine stain procedure successfully for years and have not
changed our materials or procedure in any way. However recently our sections
have been plagued by almost perfectly hexagonal shaped deposits of electron
lucent to opaque "stuff". These deposits aren't embedded within the epoxy as
they ripple and burble under a concentrated beam. Microprobe (EDXEA) of the
deposits gives a solitary lead peak (no uranium peak), but it is unclear
whether the material is an inappropriate lead deposit or another material,
that may have come from the boat or wash water, that is binding the lead
stain. Between the hexagons, we see that the embedded tissue stains well. I
would appreciate any suggestions as to what this might be and how to stop it."

I've had the same problem. Generally the problem occurs when our
sodium citrate gets too old. When we order fresh sodium citrate, we
aliquot 15-20g into separate scintillation vials and seal the vials
tightly. We use the amount necessary to make Reynold's lead citrate and
dispose of the leftover sodium citrate in the used vial. Apparently, the
reopening of a bulk container allows degradation of the sodium citrate.
Then again, this might be silly superstition and my gift of
leftover sodium citrate might be a suitable sacrifice to appease the fickle
nature of the gods of EM.



Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Thu, 1 Feb 1996 12:02:15 -0800 (PST)
Subject: call for papers, Microscopy Colloquium

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Announcement and Call for Papers

Fourth Annual Microscopy Colloquium
hosted by
California State University and
Southern California Electron Microscope Society

Saturday, May 4, 1996
Sunday, May 5, 1996

at the San Diego State University Aztec Center, San Diego California

Business meeting for CSU delegates
Friday, May 3, 1994

Abstracts Due March 15, 1996

The proceedings of the meeting will be published in
Microscopy Research and Technique.

This Colloquium provides a forum for the exchange of research interests and
experiences in all fields of light and electron microscopy, in biological,
geological, and materials sciences. Participants include students and
scientists from academia and industry. Both platform and poster
presentations are invited.

Student presentations are strongly encouraged

Registration:

Registration Fees: $35 Regular $10 Student $50 Vendor by March 15

Late Registration: $45 Regular $10 Student $60 Vendor, after March 15

Makes checks payable to: EM Facility/Colloquium

Send to: Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego, CA 92182-4614

For additional information, flyers, and abstract forms, contact:
Steve Barlow (619) 594-4523 or sbarlow-at-sunstroke.sdsu.edu


---------------------------------------------------------------------------
------------------------------------------------------------------------------
Dr. Steve Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu





From: W.Jablonski-at-csl.utas.edu.au (Wis Jablonski)
Date: Fri, 02 Feb 1996 09:26:19 +1000
Subject: Crystallographic Congress 1996

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Dear All,
Thank you very much for helping me to locate Bill Miller and especially
Prof. Summers, Irving Delilah and Susan Udin.Thanks again.
Cheers, Wis Jablonski , CSL, Uni-Tas, Australia





From: Cannon9965-at-aol.com
Date: Thu, 1 Feb 1996 19:51:44 -0500
Subject: Objective lens cleaning

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I was advised by my microscope rep. (a very respected microscopist) to use a
heavy polishing compound (like used to remove paint on cars) and light grit
sandpaper to remove oil and lens coatings on our lenses. On particularly
difficult occasions a sandblaster has sufficed. Needless to say that in 23
years we have experienced no difficulty using this method. We have never
published a paper based on our findings but we hope to get a Science article
soon.

I thought that with the huge influx of postings regarding this issue I would
just add my 2 cents worth as it seems everybody had a different idea about
how to do it. You should contact you local microscope rep. if you need to
know how to clean a lens.




From: Murphy, Judy :      murphy-at-sjdccd.cc.ca.us
Date: 1 Feb 1996 17:14:08 -0800
Subject: SEM/EDX Technologist Opening

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Message-ID: {n1388927599.95193-at-sjdccd.cc.ca.us}
"MSA List" {microscopy-at-aaem.amc.anl.gov} ,
"EM Lab" {emlab-at-sjdccd.cc.ca.us} ,
"Hinds, Joan" {jhinds-at-sjdccd.cc.ca.us} ,
"Villalovoz, Frank" {fvillalovoz-at-sjdccd.cc.ca.us}
X-Mailer: Mail*Link SMTP-MS 3.0.2


Altera Corporation
SEM/EDX Technologist (Reliability Technician Opportunity)


COMPANY: Altera Corporation is a leader in advanced programmable logic
devices and software. Our continued growth and success necessitates the need
for the
following talented individual to join our team.

JOB DESCRIPTION: In this position you will work with product, reliability and
technology engineers to identify appropriate failure analysis techniques,
provide physical analysis service to identify the root causes of the failures,

document the results, and inform the engineers of the root causes for
corrective actions. You will also coordinate the maintenance of failure
analysis equipment.

QUALIFICATIONS: The ideal candidate will have one plus years related
experience in physical analysis. Also they will be familiar with usage of
analysis equipment including SEM, EDX, precision cross sectioning, RIE, Jet
Etcher and chemical de-processing set-up. Good communication skills, ability
to handle multiple projects and interact effectively with others is a must.

BENEFITS: Altera offers a challenging environment along with comprehensive
benefits which include profit sharing, company matching 401(k) plan and an
Employee Stock Purchase Plan.

CONTACT: Principals only please. EOE.
Send resume to:

Altera Corporation,
Human Resources,
Attn: JG/SJDC295,
2610 Orchard Parkway,
San Jose, CA 95134-2020.
FAX: 408-435-5065.







From: rlmarkgraf-at-ucdavis.edu (Rick Markgraf)
Date: Thu, 1 Feb 1996 14:16:37 -0800
Subject: Re: cleaning LM lenses

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} Diana van Driel wrote:
} I was recommended to clean any gunked up lens with a knob of polystyrene
} foam - no chemicals, just the dry foam rubbed in a circular motion onto the
} lens. Over the years none of my Zeiss lenses has suffered any damage from
} this.

Have you inspected the front lenses of your objectives under
magnification to see if there are scratches in the coating? If there are
none, is is a testament to the durability of the Zeiss coatings.

I have seen too many microscope lenses (yes, even Zeiss) marred by
rubbing with dry materiels. I don't recommend this for any lens. The dust
particles that settle on lenses, particularly uncovered oculars, act like
sandpaper when rubbed across the lens surface. Solutions such as lens
cleaners lubricate these particles and reduce their abrasive effect. Cotton
buds tend to lift the particles into their fibers, while, I believe,
polystyrene would tend to keep them at the lens surface where they can do
damage.

This discussion of cleaning gunked immersion lenses misses the real
problem, in my mind. I care for 2100 microscopes constantly in use by
university students. We use only synthetic immersion oils. Despite
warnings, a certain number of these microscopes end up with immersion oil on
the high dry (40X) objectives, rendering them useless until cleaned. Since
the microscopes may be of 8 different brands, of varied models and ages, my
cleaning method has to work for them all, and be applicable in a classroom
situation. Cotton buds and glass cleaner (Windex or something similar) used
in a wash and dry pattern, work well for cleaning all lenses and is
relatively benign to microscope and student. Glass cleaner doesn't work
well for immersion oil on the 40X, because it requires repeated application
of the glass cleaner to remove all traces, a time-consuming effort.
The US Government, and particularly the state of California, have
stringent safety rules governing use of chemicals. While xylene, may be the
best cleaner for immersion oil, its toxicity is a drawback and I hesitate to
recommend it for students in a classroom environment. Same goes for pet
ether, methyl-ethyl ketone, acetone or chloroform. Alcohol would be a
preferred solution, but, because of warnings about its use with older
microscopes, I hesitate to recommend it. Perhaps when the older microscopes
are completely phased out of our inventory ...
Immersion oil seems to be a popular thread with this list. I would
appreciate any comments or ideas.

Rick Markgraf
Microscope Services
University of California, Davis
rlmarkgraf-at-ucdavis.edu
PH. (916)752-3477 FAX (916)752-6363







From: vpdravid-at-casbah.acns.nwu.edu
Date: Thu, 1 Feb 1996 23:23:46 -0600
Subject: Position Open

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"Matthew Libera" {mlibera-at-vaxa.stevens-tech.edu} ,
"Eric Lifshin" {lifshin-at-crd.ge.com} , "Charlie Lyman" {cel1-at-lehigh.edu} ,
"Tom Malis" {Tom.Malis-at-cc2smtp.emr.ca} , "Jon McCarthy" {jonm-at-noran.com} ,
"Stuart McKernan" {stuartm-at-maroon.tc.umn.edu} ,
"Mike Miller" {xkm-at-ornl.gov} , "John Mansfield" {jfmjfm-at-umich.edu} ,
"Joe Mayer" {jmayer-at-vaxww1.mpi-stuttgart.mpg.de} ,
{Microscopy-at-aaem.amc.anl.gov} , "Joe Michael" {jrmicha-at-sandia.gov} ,
"Terry Mitchell" {temitchell-at-lanl.gov}

Dear Friends:

There is an immediate opening in our group at Northwestern
University for a Postdoctoral Scholar or a Research Associate, in the area
of Analytical Electron Microscopy/Materials Science. I would greatly
appreciate it if you could pass on the following information to interested
parties. Please have anyone interested contact me at the
address/e-mail/phone below.

Thanks !

Vinayak
============================================================================

POSITION OPEN: Postdoctoral Scholar or Research Associate

Area: Analytical Electron Microscopy & Materials Science

Qualification: A PhD in Materials Science/Physics or related area. Very
strong hands-on experience in various AEM techniques is required, including
transmission EELS, x-ray microanalysis and CBED. Experience in specimen
preparation of variety of materials, especially x-sectional TEM is
required. Preference will be given to those who have demonstrated skills
and publications using FEG TEM/STEM/SEM and interfacial/defect phenomena in
solids.

Instrumentation available at NU in the newly restructured Electron
Probe Instrumentation Center (EPIC) includes: A Hitachi HF-2000 FEG
TEM/STEM with x-ray, EELS, in-situ IV/LHe holder and e- holography set-up,
a Hitachi S4500-II FEG SEM with x-ray, EBSP/OIM and LHe stage with in-situ
IV probes, a Hitachi H8100 cTEM, and access to various other TEMs/SEMs and
other analytical instrumentation.

Research involves use of diverse AEM techniques to probe problems
of thin film growth, interfaces & grain boundaries in oxide systems.

The position is open immediately for at least one year, renewable
upon mutual agreement for longer period. Salary and benefits will
commensurate with experience and skills.

Please contact immediately:

*******************************************************
(Vinayak P. Dravid)
Associate Professor, Materials Science & Engineering
Director, Electron Probe Instrumentation Center (EPIC)
Northwestern University, Evanston, IL 60208, USA
Ph.: (847) 467-1363, Fax: (847) 491-7820
E-mail: v-dravid-at-nwu.edu
*******************************************************


Thanks,

(NOTE THE NEW AREA CODE)
*******************************************************
(Vinayak P. Dravid)
Associate Professor, Materials Science & Engineering
Director, Electron Probe Instrumentation Center (EPIC)
Northwestern University, Evanston, IL 60208, USA
Ph.: (847) 467-1363, Fax: (847) 491-7820
E-mail: v-dravid-at-nwu.edu
*******************************************************






From: DonPfield-at-aol.com
Date: Fri, 2 Feb 1996 07:50:17 -0500
Subject: XRF Upgrade

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Thanks for all the help. I will contact each suggestion.

DonPfield




From: Michaela Just :      MICHAELA-at-MOON.OVI.AC.ZA
Date: Fri, 2 Feb 1996 14:54:46 CAT
Subject: unsubscribe

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unsubscribe microscopy newsgroup, thanx
Michaela Just
Onderstepoort Veterinary Institute
P.O. Box x05
Onderstepoort
0110
RSA
e-mail: Michaela-at-moon.ovi.ac.za
Tel: 27-12-5299215
Fax: 27-12-5299434




From: kelloes-at-emlab.cb.uga.edu
Date: Fri, 2 Feb 1996 11:54:10 +0000
Subject: Short (8-15 pages) Electron Microscopy Article

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Hello on the Net:

I need help in either getting articles or article references
on a good, short EM paper that would be useful for teaching
students. In the July-August 1992 American Scientist Magazine,
there was an excellent article, entitled "The New Vision of Light
Microscopy". This is an excellent paper on LM and image analysis
for students and also for technician references. If anyone out
there has any suggestions or ideas, please let me know. Thank you in
advance. Cathy Kelloes




From: Leo Marin :      leo-at-spine.med.utoronto.ca
Date: Fri, 2 Feb 1996 10:34:36 -0500 (EST)
Subject: Heat Pen

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In the past I have used chloroform routinely for expanding thin sections.
Because of the hazardous nature of this chemical I tried a heat pen
(amps - .125), but it was totally ineffective. Would a more powerful
heat pen be effective in reducing compression ? I would appreciate
some feed back on this.
Thanks
Leo




From: John Gabrovsek :      gabrovj-at-cesmtp.ccf.org
Date: Fri, 02 Feb 1996 10:31:02 -0500
Subject: TEM,Staining problem

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While discussion is going on about the precipitate that is forming on
grids even when ultrapure boiled water is used (Cartwright, Lovett).
I have another question. I see sometimes grids jumping in plastic
dish becose of static electricity. I imagine that if we place charged
grid on stain solution that some stain will precipitate.Is out there
anybody who would comment for this possibility?
John Gabrovsek





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Fri, 02 Feb 1996 10:59:27 -0600
Subject: Re: stain problem

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At 04:28 PM 2/1/96 -0600, you wrote:

"Do you wet your grids before putting them in your methanolic uranyl acetate?"

No, we haven't been wetting the grids first. But that sounds like a
reasonable thing to do. If Mollenhauer suggested it, it must be good.

*********************

"I also have one caution about using acetic acid to clean grids. If you
leave the grids in the acetic acid too long, you'll get flakes of copper on
your sections."


Yes, we're careful about that. One thing that may be contributing to the
problem is that we're using the thin bar "Super 200" grids because we need
to maximize the open space. These grids are difficult to wash as thoroughly
as we'd like because there is less metal for the sections to adhere to and
they are easily washed off the grid. But we've been using these grids and
stain procedure successfully for years. We are looking for things in the
laboratory environment that have recently changed. Our pay scale is not high
on the list of suspects.

Thank you, Jane.

* * Joiner Cartwright, Jr. * *





From: Shea Miller :      MILLERS-at-em.agr.ca
Date: Fri, 02 Feb 1996 09:21:32 -0500
Subject: freeze substitution to retain soluble components

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Greetings all!
I am trying to use freeze substitution to retain soluble
oligosaccharides in plant tissues, and am not yet convinced that I
have been successful, partly because of embedding/infiltration
problems (I think!). We have tried a variation of the tannic acid
fixation with Kaeser's substitution cocktail, and stuff seems to
look ok (tough to really assess when the scope has been down,
unfortunately), although we have been advised that probably LR
White is easier to work with than Lowycryl K4M, which is what we
used the last time, and it doesn't section happily. Would like to
try another run (just got some fresh LR White) and thought it
would be useful to ask for some input from the group. It's
probably of interest to more than me, but if folks prefer to
respond directly, I will summarize the responses and post them.
I'm certainly looking forward to your input!

TIA
shea

S.Shea Miller
Agriculture and Agri-Food Canada
Centre for Food and Animal Research
Central Experimental Farm
Ottawa, Ontario
Canada K1A 0C6
Phone: 613-759-1760
Fax: 613-759-1765
email: millers-at-em.agr.ca





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Fri, 02 Feb 1996 14:14:01 -0600
Subject: Re: Stain problem

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At 08:55 AM 2/2/96 -0800, you wrote:

} Could the UA somehow have been cross-contaminated with Pb?
} Sometimes a weak wash with 0.02 N NaOH will help with Pb deposits-if that
} is the source of the problem.
}
}
} Doug Davis
}
**********************
Doug,

We've been pretty careful not to cross contaminate. We may have to resort to
after-the-fact cleaning, but I would like to eleminate the problem before it
occurs. Thanks for the suggestion.

* * Joiner Cartwright, Jr. * *





From: Andy Blackwood
Date: Fri, 2 Feb 1996 12:00:15 -0500
Subject: Audit at U of Hawaii

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2 February 1996


To: Microscopy BB

I think there is something about the circumstances described over the last
few days by Tina Carvalho that recalls some of the recent discussion of
ethical values.

No matter how high and noble our calling, we all work within rules. Some of
them are institutional, some professional and some come from the laws of our
state and our nation. Whatever their source, however, the rules are what the
rules are.

If we don't like the rules, we can work to change them, but none of us has a
license to violate them. Auditors, internal or external, are in the business
of finding violations of the rules, and some of them do a very good job of
doing just that. All of us are tempted from time to time to bend or to break
the rules under which we operate, sometimes for a very good purpose. In the
case of our microscopy supplies business, for example, we may be asked to
sell the components of a sputter coating system separately so that they can
be bought out of a budget intended for consumables, when the rules say that
the system is capital equipment, to come out of another budget. Is this
right? Or is it a conspiracy to defraud? Does the noble purpose (the fact
that the funds will go back to the sponsor) make any difference? How about
the request that we predate or postdate the invoice, to get it into the
"right" year?

It seems to me that we are all better off if we just force ourselves to live
within the rules instead of trying to rationalize our efforts to get around
them.

BTW, am I the only person who wonders which is the chicken and which is the
egg when Tina comments about the lack of commercial EM facilities within her
state?

Andy

Andrew W. Blackwood, Ph.D.
Structure Probe, Inc.
63 Unquowa Road
Fairfield, CT 06430-5015
Ph: 1 203 254 0000
FAX: 1 203 254 2262
e-mail: AWBlackwoo-at-aol.com
WWW--http://mail.cccbi.chester.pa.us/spi/spihome.html




From: CSENCSITS-at-aaem.amc.anl.gov
Date: Fri, 2 Feb 1996 16:55:14 -0600 (CST)
Subject: Pt TEM grids

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Does anyone make Pt TEM grids? They are needed for high temperature
experiments-other grids oxidize.

Roseann Csencsits
Argonne National Laboratory
Argonne, IL
708-252-4977




From: CSENCSITS-at-aaem.amc.anl.gov
Date: Fri, 2 Feb 1996 16:53:52 -0600 (CST)
Subject: JEOL 4000-double tilt holders

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Does anyone know where I might be able to purchase some used double tilt
top entry specimen holders for a JEOL 4000 EX? Is there someone
decommissioning a JEOL 4000EX that would like to sell their specimen holders?

Roseann Csencsits
Argonne National Laboratory
Argonne, IL
708-252-4977




From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Fri, 2 Feb 1996 16:16:11 -0500 (EST)
Subject: TEM Embedding question (fwd)

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Does anyone have a protocol for using (HPMA?) instead of propylene oxide and
embedding coverslips directly in cell wells, and then using liquid
nitrogen to remove the coverslip after plastic is polymerized?





From: Paul Webster :      Paul.Webster-at-quickmail.yale.edu
Date: 2 Feb 1996 17:40:07 -0500
Subject: Re: freeze substitution

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Message-Id: {n1388839485.36053-at-QuickMail.Yale.edu}

Dear Shea,

Have you considered fixing the tissue in aldehyde and embedding in one of the
other (hydrophobic) Lowicryl resins (e.g. HM 20, HM 23). They polymerize at
lower temperatures and cut very well. We have had some success with soluble
proteins in the pancreas and there are published examples of this method.

Here are a few references which might be of interest:
van Genderen et al, 1991 J. Cell Biol. 115:1009-1019.
Oprins et al, 1994 J. Histochem. Cytochem. 42:497-503.

There is also a recent paper in Microscopy Research & Technique which covers
this subject but omits to refer to the above papers. The paper is in MRT, 1996
33:251-261. Look too at the work by Humbel & Schwarz which is cited in the MRT
paper.





From: :      oliver-at-ipas2.afip.mil
Date: Fri, 2 Feb 1996 17:26:17 -0500 (EST)
Subject: Re: Audit at U of Hawaii

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On Fri, 2 Feb 1996 AWBlackwoo-at-aol.com wrote:

} 2 February 1996
}
} From: Andy Blackwood
}
} To: Microscopy BB
}
} I think there is something about the circumstances described over the last
} few days by Tina Carvalho that recalls some of the recent discussion of
} ethical values.
}
} No matter how high and noble our calling, we all work within rules. Some of
} them are institutional, some professional and some come from the laws of our
} state and our nation. Whatever their source, however, the rules are what the
} rules are.
}
} If we don't like the rules, we can work to change them, but none of us has a
} license to violate them.

On the contrary. The idea that the existence of arbitrary rules defines
ethical behavior is incorrect by construction. The classic examples,
of course are in the realm of human rights violations -- where the
ethical actions are explicitly those which are against the rules imposed
by an unethical or irrational government.

It is *not* necesarily unethical to break rules, particularly
when those rules are arbitrary, capricious, or in themselves
unethical.


} It seems to me that we are all better off if we just force ourselves to live
} within the rules instead of trying to rationalize our efforts to get around
} them.
}

When you call efforts to get around irresponsible regulations
"rationalization" you beg the question that sometimes getting
around the regulation is the only ethical thing to do. Sometimes
finding an excuse for observing the regulations, and thus avoiding
actions which involve personal risk, is the rationalization.

Ethics and rules are not orthogonal, but I, at least, refuse to
let arbitrary bureacrats and supercilious functionaries get away
with calling me "unethical" if I am not sufficiently obsequious
to their dictates.

billo




From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Fri, 2 Feb 1996 20:41:03 -0500
Subject: Re: More on database S/W

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Message-Id: {v02120d01ad3866b7ccdb-at-[128.174.23.207]}
Mime-Version: 1.0
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} Considering the number of comments and questions I received concerning my
} recent recommendation of Q&A database software...
} Don Grimes, Microscopy Today

I have used Q&A, and must agree with Don Grimes. Most database
programs have a lot of unnecessary bells and whistles.

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Fri, 02 Feb 1996 23:50:36 EST
Subject: Platinum grids

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On February 20 was written:

} Subject: Pt TEM grids
}
} Does anyone make Pt TEM grids? They are needed for high temperature
} experiments-other grids oxidize.
}
} Roseann Csencsits
} Argonne National Laboratory
} Argonne, IL
} 708-252-4977
}
}
Pt grids are not in our printed catalog but are to be found in our all-
electronic catalog on our WWW website as indicated below, in several
different mesh sizes.


Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: HENRY P ADAMS :      hadams-at-nmsu.edu
Date: Sat, 3 Feb 1996 10:36:17 -0700 (MST)
Subject: DBC box for Hitachi H7000

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I am in the proces of purchasing a digital image acquisition system for a
Hitachi H7000 stem. To do this I need a digital beam control box to
interface the microscope to the acquisition system. The later models, eg.
Hitachi H7100 have this circuitry built in but the older models (H7000)
need the DBC box which Hitachi made but no longer does. Does anyone
out there have one that they don't use anymore or know of any extras
collecting dust on a shelf somewhere? I am slightly desparate and have
some money (not much) to pay for such a beast.
Thanks in advance for info or sources,
Hank Adams
EML, New Mexico State Univ
email: hadams-at-nmsu.edu
phone: 505/6463600




From: emccjb-at-ttuhsc.edu (Charles J. Butterick)
Date: Sat, 3 Feb 1996 11:44:45 -0600
Subject: Re: Audit at U of Hawaii

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Andrew W. Blackwood, Ph.D. Structure Probe, Inc. wrote:
"......It seems to me that we are all better off if we just force
ourselves to live within the rules instead of trying to rationalize our
efforts to get around
them........".

Andy is correct. The rules and laws must be followed. Those we
don't agree with we can work to change. But am I the only one who reads
Andy's entire commentary (that only mentions what academics do to get
around some regulation in order to purchase an item) and feels resentment?
I have been approached many times by vendors who ask me to write
lock-out specifications so I will buy from only one source. Other vendors
have informed me that if I bought a certain dollar amount of supplies, they
would throw in the instrument for free that uses those supplies. More than
once I have successfully appealed to the State of Texas on a bid where an
unscrupulous vendor misrepresents their instrumentation and/or the price.
Some vendors are as eager to earn the academic dollar as the academic is to
stretch what little he/she has.
What about vendors/business people who appeal to the government
and/or granting agencies to restrict the activities of the academics, thus
reducing competition? Chuck Garber will readily inform you of the meeting
that promulagated the rule that says instrumentation purchased with federal
dollars cannot be used for commercial purposes. When that rule was made,
there was no academic representation (the perspective of scientists at
NSF/NIH is different than that of scientists in colleges and universities).
Is that fair? What about the politically incorrect white vendor(s) who set
up, or use, minority/women fronts in order to sell their products? Is
there any noble purpose for vendors who abuse the system for profit and
gain?
A more important question would ask why is there such a strident
militancy against academics among certain individuals at SPI? Let it out,
guys, so maybe we can feel your pain......

Chuck




Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: AMCGroup2-at-aol.com
Date: Sat, 3 Feb 1996 22:21:16 -0500
Subject: Re: Anonymous Inquiry

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In response to the inquiry from anonymous subscriber,
ratyrg-at-esvax.dnet.dupont.com, posted on 96-01-24 (regarding availability of
cryomicrotomy courses), please be advised that AMC Group offers a series of
intensive hands-on workshops on cryomicrotomy on a regular basis. We also
offer other advanced workshops including the FIB cross-sectioning and
wedge-polishing for site-specific TEM specimen preparation. For the
workshops schedule and other informative material on the general subject of
specimen preparation, please see our page on WWW at the Microscopy-Online
site (http:// www.Microscopy-Online.com/). For further information, please
contact me directly. Thanks.

Renee E. Nicholas
Workshops Coordinator
AMC Group




From: Arkadiusz Kloc :      al969-at-freenet.toronto.on.ca
Date: Sun, 4 Feb 1996 14:51:40 -0500 (EST)
Subject: unsubscribe

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Please "unsubscribe" me
- Thank You




From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Sun, 4 Feb 1996 13:03:54 -0500
Subject: Re: Audit at U of Hawaii

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} What about vendors/business people who appeal to the government
} and/or granting agencies to restrict the activities of the academics, thus
} reducing competition?
} Charles J. Butterick (Chuck)

Or, as an actual experience, the SEM vendor who threatened--the
correct word--to file a complaint with US Customs, and force a *previous*
employer to pay a multi-thousand-dollar penalty if we didn't buy a US-made
SEM.
(Needless to say, we didn't, and they didn't, since it was a
fatuous threat. And a permanent loss of business.)
Phil Oshel
(Not at U. Ill. at above the time)

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: Arkadiusz Kloc :      al969-at-freenet.toronto.on.ca
Date: Sun, 4 Feb 1996 14:53:11 -0500 (EST)
Subject: Unsubscribe

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Please "unsubscribe" me
-Thank You




From: MicroToday-at-aol.com
Date: Sun, 4 Feb 1996 17:36:11 -0500
Subject: Microscopy Images/Prints

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Group:
I am sure that many of you are familiar with the nifty microscopy images
produced by David Scharf. If not, allow me to note that they have been
featured in Life, Time, National Geographic, Scientific American, Discover,
Natural History, Harpers, The Saturday Evening Post, etc.
As it happens, I am in the process of producing a series of 16 of his
best images - typically as 23 in x 19 in, HIGH quality, lithographic prints.
Descriptions, etc. of the series is contained in the next issue of
Microscopy Today, which will be mailed in a week's time. Should you,
including our international associates, not be receiving the newsletter and
have a potiential interest in the prints, kindly advise by eMail and I would
be delighted to send you a no cost description of the series.
And, should the publication of microscopy prints be an "enjoyable"
hobby, I may extend the series. Should you have microscopy images that you
consider worthy of reproduction, I would appreciate hear from you.
Understanding that there is a bit of "commercialism" in this note, I
hope that the uniqueness of the subject causes not many of you to be
offended.
Best regards to all,
Don Grimes, Microscopy Today






From: Mobiewan-at-aol.com
Date: Sun, 4 Feb 1996 19:43:46 -0500
Subject: SEM for sale

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Do you know anyone who might be interested in an ISI Super III-A SEM that's
in excellent operating condition for only $8,000? We are in the St. Paul, MN
area and would be happy to demo the scope in our location on your specimen.
This workhorse would be an excellent instrument for a small
college/lab/hobbyist or manufacturer. Contact me for more specs or info.
Ask me about a $300 travel rebate offer.

Mark Overmyer
Kevmar Equipment Co.




From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Mon, 5 Feb 1996 11:53:02 +1100
Subject: Re: Heat Pen

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} In the past I have used chloroform routinely for expanding thin sections.
} Because of the hazardous nature of this chemical I tried a heat pen
} (amps - .125), but it was totally ineffective. Would a more powerful
} heat pen be effective in reducing compression ? I would appreciate
} some feed back on this.
} Thanks
} Leo

The heat pen I use is fine as long as it is held close to the section and
used for around 30 secs (longer for some sections).


Diana van Driel
Dept Ophthalmology
Sydney University 2006
NSW, AUSTRALIA






From: Keith Moulding :      mcmouldk-at-uxmail.ust.hk
Date: Mon, 05 Feb 1996 15:40:10 +0800
Subject: Sixth Asia-Pacific Conference On Electron Microscopy

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Sixth Asia-Pacific Conference On Electron Microscopy.

Under the Auspices of The International Federation of Societies
for Electron Microscopy (IFSEM) and The Committee of Asia-Pacific
Societies for Electron Microscopy (CAPSEM).

The Sixth Asia-Pacific Conference on electron Microscope will be
held at the Chinese University of Hong Kong, to be held on
July 1-5, 1996.

Final call for abstracts.

The deadline for abstracts has been extend to 15th February, 1996.
If you are close to the deadline, please use an express mail service
to submit your abstracts. Faxed abstracts cannot be accepted.


The conference will provide a forum for the dissemination of new
information on the application of scanning and transmission electron
microscopy, diffraction and microanalysis in life and materials
sciences. The conference will include scientific sessions (plenary
lectures, symposia oral presentations and posters). There will also
be an exhibition of scientific instruments together with workshops
and open laboratories.

*******************************************************************
Contact address:

The 6APEM conference,
Secretariat,
Chintek Promotion Services,
13/C Trust Tower,
68 Johston Road,
Wanchai,
Hong Kong.

FAX: (852) 28611022, attention Ms. Karina Ng.
(852) 25296045


*******************************************************************
Please contact the above address only. Please do not send requests
for information to this e-mail address.





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Mon, 05 Feb 1996 11:01:57 -0600
Subject: Re: Staining Problems

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Message-ID: {199602051315.IAA18418-at-IndyNet.indy.net}
To: Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

At 02:41 PM 2/4/96 -0500, you wrote:

} Dear Joiner,
} If you find a solution to your staining problems, could you please let me
} know? I have used the same receipe for UA and LC for over 20 years without
} problems. But recently, have been haunted by the symptoms you relayed, with
} always using ddwater and trying NaOH washes, different grid suppliers... I am
} ready to record the position of the planets when I get good staining. I
} wonder if such can be related to low humidity and increased static
} electricity???????????? I hope you are able to solve this curse and that you
} will share such with me. Needless to say, I will let you know if I have any
} luck.
}
} Best regards,
}
} Robyn Rufner, Ph.D.
} Deborah Research Institute, 20 Pine Mill Rd., Browns Mills, NJ 08015
} 609-893-1016, fax: 609-893-2441, email: rrufner-at-aol.com
}
********************
Robyn -

I will indeed share what I've gleened from this thread, but as I told Dwight
Beebe, not until you all have solved my problem. No, actually I will pass it
on. That's what this listserver is for. The static charge sometimes found on
grids has been suggested to be causing precipitation of the stain. If I can
find one of those antistatic guns that we used to de-charge our LP's with,
I'll try it, being the student of reason and logic that I am and staunch
supporter of the scientific meathod. Then at the next full moon I'm going to
nail a dead cat to the north side of an oak tree....

* * Joiner Cartwright, Jr. * *





From: John_R_Reffner-at-rohmhaas.com (John R Reffner)
Date: 1/31/96 5:58 AM
Subject: CD ROM for archiving

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Mime-Version: 1.0
Pogany Lajos {pogany-at-power.szfki.kfki.hu}
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
Content-Description: cc:Mail note part

I'm also looking in to CDR's for archiving. I think we will get the Pinnacle
5040, a 2x write 4x read, which runs about $1300 ($1000 internal). HP makes
one for about the same cost, Yamaha and Sony have models for a bit more $.

Any comments from people already using any of these?


______________________________ Reply Separator _________________________________


Dear subscribers,

Is anyone able to assist me in finding a CD ROM writer for image archieving
in the price range of about 1000 $?
Would be possibile to send me addresses where are those available?

Thanks for Your help

LAjos Pogany
pogany-at-power.szfki.kfki.hu






From: Steinmeyer-at-mbox.biophysik.uni-hannover.de
Date: Mon, 5 Feb 1996 18:14:12 +0100 (MET)
Subject: Ca2+-Measurements with fura

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Hello all,

does anyone know if there is a newsgroup or a listserver discussing problems
of measuring Ca2+ with fura?
Perhaps a group dealing with fluorescence microscopy in general would help.

Thanks for any help

--
Ralf Steinmeyer (Steinmeyer-at-mbox.biophysik.uni-hannover.de)
UNI Hannover Herrenhaeuser Str. 2
Inst. f. Biophysik 30419 Hannover




From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 5 Feb 1996 10:03:53 -0800 (PST)
Subject: Re: cleaning LM lenses

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X-Sender: glenmac-at-homer20.u.washington.edu

We use Kodak lens cleaner for routine cleaning, plastic dropper bottles
are refilled from the more affordable quart containers. Chloroform is
reserved for obstinate deposits, like when someone immerses a non-DPX
lens into the DPX or Permount.

A couple of microscope technicians that have worked on our equipment use
lighter fluid. It dries without leaving a film and yet is claimed not to
attack lens adhesives or coatings. Has anyone else used lighter fluid on
lenses? Personally, I've only used to take scuff marks of vinyl
flooring.

Regards,


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu










From: William R Oliver :      oliver-at-ipas4.afip.mil
Date: Mon, 5 Feb 1996 17:43:21 -0500 (EST)
Subject: Re: Ethics

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On Mon, 5 Feb 1996, Don Chernoff at ASM wrote:

} Recent messages by Blackwood and Oliver are examples of two opposing points
} of view about ethics and philosophy. On one side is the normative view:
} that there exist absolute standards ('norms') for conduct. On the other
} side is the relative view: standards depend on the situation.

I'm not so sure that it's a question of absolute versus situational
ethics as much as *who* defines the "right" and "wrong." My position
is not that ethics are situational; my position is that bureaucratic
rules and ethics are not the same. Thus, noncompliance is not prima
facie evidence that someone has done something "wrong." My primary
concern is with the increasing criminalization of noncompliance, leading
to allegations of "fraud," "scientific misconduct," and such.

I am not a scofflaw, and I certainly try to live within rules within
reason. However, I don't mistake regulatory pronouncements for divine
dispensation. Not confusing the two does not equate to the support of
anarchy, nor does necessarily make it easy to rationalize any actions,
as a different respondent suggests. Indeed, I might suggest the
opposite -- that recognizing and accepting individual responsibility
for individual ethical decisions independent of an external rulemaking
bureaucracy might lead to more careful consideration of action, not less.
Relying on legalistic justifications is perhaps not the last, but certainly
one of the favorite refuges of a scoundrel.

} Let me remind the reader that we are not talking about human rights
} violations, tyranny or murder here. We are only talking about money. It
} really isn't worth doing more than 'blowing off steam'. I say follow the
} rules that exist and work to change the rules that appear improper.

You bring up another interesting idea, that we are not talking about
human rights, but, instead, "We are only talking about money."

In fact, we *are* talking about human rights. It isn't "only money" if a
governmental agency, by its regulatory or enforcement rights, deprives you
of your livelihood, appropriates your possessions, and criminalizes
violation. When investigations result in allegations of fraud there is
resultant criminal liability; the only question then is whether or not the
regulatory agency wants to take you down bad enough to expend the effort.
It may be only money, but when legal fees and seizures destroy life
savings it is more than a mere inconvenience. When allegations of fraud
or "scientific misconduct" based on technical violations of regulatory
pronouncements leads to the destruction of a career, it is a nontrivial
life event. For me, at least, it would not be "only money" at risk. As
ex-Secretary Donovan said after the trials that destroyed his career in
spite of finding him innocent, "Where do I go to get my reputation back?"

When the time and effort it takes to attempt to satisfy the voracious
appetitite of a regulatory agency detracts from scientific investigation,
patient care, or stifles innovation and experimentation, it is a loss to
more than a single investigator. When regulatory demands and limitations
force new intellectual exploration and development off the shores of the
US, it hurts this nation badly.

In spite of all of this, I would take a more sanguine attitude about
today's regulatory atmosphere were it not for the problem that, in my
personal opinion, in many areas the regulatory morass is so byzantine and
self-contratictory that it is fundamentally impossible to remain in
compliance given limited time and resources to devote to doing so. In
many a regulatory setting, particularly an investigation, the person being
investigated is by no means considered "innocent until proven guilty."
Instead, the assumption is that the worst possible interpretation is the
correct one, and it is the burden of the person being investigated to
prove his or her innocence. And the investigators have all the
resources. It is no surprise that even the innocent plea bargain when
they face financial ruin in the face of defending themselves against the
charges of "fraud," "conspiracy," etc., etc., etc.

In such an atmosphere, simply trying to do one's best is just not good
enough. A person who thinks he or she is working within the rules simply
doesn't know all the rules. There are no innocent, only the untargeted,
and obscurity is a weak defense.

Of course, I could be wrong.

billo







From: WARRENJ1-at-cliffy.polaroid.com
Date: 2/3/96 1:44 PM
Subject: Re: Audit at U of Hawaii

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I believe that I am one of the vendors that Chuck Butterick is
referring to that offered *free* equipment with the purchase of a
certain amount of supplies. It has been a a year or two since I
offered the program to Chuck.

He is correct that the idea was to get around budget rules that allow
unlimited purchase of supplies but no capital or equipment purchases.
In this particular instance, if you bought the equipment and the
supplies(film, in this case), paying the lowest available pricing, you
would spend the same $ as you would if we gave you the hardware and
you paid the premium price for the requisite cases of film.

I will admit that we have a vested interest in placing our equipment
with customers so that we sell film. However, I would suggest that if
our customers have a need for any of our products and due to various
constraints are unable to acquire it through equipment purchase
funding, we are working to meet the customers needs-we cant make the
customer invest in equipment or participate in a program if they do
not want to.

Yes, I am in sales and my livelihood depends on what I sell. I believe
that what I sell offers my customers a better way of doing their
job;more effectively and more efficiently-if I didnt, I'd quit.

Certainly I would agree that some vendors may have unscrupulous
practices and I do not condone that. I am asking you to consider that
the intent behind our program and possibly others is to benefit you-so
you can have the tools you need to do your job.

John D. Warren
Eastern US Sales Manager
Helios Scientific Group
Polaroid Corporation
______________________________ Reply Separator _________________________________


Andrew W. Blackwood, Ph.D. Structure Probe, Inc. wrote:
"......It seems to me that we are all better off if we just force
ourselves to live within the rules instead of trying to rationalize our
efforts to get around
them........".

Andy is correct. The rules and laws must be followed. Those we
don't agree with we can work to change. But am I the only one who reads
Andy's entire commentary (that only mentions what academics do to get
around some regulation in order to purchase an item) and feels resentment?
I have been approached many times by vendors who ask me to write
lock-out specifications so I will buy from only one source. Other vendors
have informed me that if I bought a certain dollar amount of supplies, they
would throw in the instrument for free that uses those supplies. More than
once I have successfully appealed to the State of Texas on a bid where an
unscrupulous vendor misrepresents their instrumentation and/or the price.
Some vendors are as eager to earn the academic dollar as the academic is to
stretch what little he/she has.
What about vendors/business people who appeal to the government
and/or granting agencies to restrict the activities of the academics, thus
reducing competition? Chuck Garber will readily inform you of the meeting
that promulagated the rule that says instrumentation purchased with federal
dollars cannot be used for commercial purposes. When that rule was made,
there was no academic representation (the perspective of scientists at
NSF/NIH is different than that of scientists in colleges and universities).
Is that fair? What about the politically incorrect white vendor(s) who set
up, or use, minority/women fronts in order to sell their products? Is there
any noble purpose for vendors who abuse the system for profit and gain?
A more important question would ask why is there such a strident
militancy against academics among certain individuals at SPI? Let it out,
guys, so maybe we can feel your pain......

Chuck




Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu




From: Richard.Larker-at-mb.luth.se (Richard Larker)
Date: Mon, 5 Feb 1996 17:32:04 +0100
Subject: TEM spec. prep. by Gatan Disc Grinder on SBT diamond films

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This is a request for information from colleagues experienced with the
thinning of TEM specimens held by a Gatan Disc Grinder against South Bay
Technology (or 3M Imperial) diamond abrasive films. First some information:


My TEM specimens are taken from joints that have been diffusion bonded by
Hot Isostatic Pressing, using the method described in J. Mater. Sci. 29
(1994) 4404-4414. The diffusion couples are as paired semicylinders fitted
into a small metal tube (3 mm outer diameter) of stainless steel or nickel.
This tube acts as an encapsulation for the isostatic argon pressure (200
MPa) during HIP, and afterwards the capsule is simply sliced with diamond
saw before thinning,leaving the capsule as a stabilizing collar around the
diffusion couples avoiding cracking for materials having very different
coefficients of thermal expansion, such as silicon nitride and Ni-base
superalloys. The materials joined so far include Ni-base superalloys (both
single crystal and polycrystalline), silicon nitride, titanium nitride, as
well as CMC (Si3N4/TiN) and MMC (TiN/Ni) compositionally graded materials
(FGM´s).

In June 1995, I participated in a very informative TEM Spec. Prep. Course
(lead by Ron Anderson, IBM, East Fishkill Lab, NY, USA) held at BAL-TEC AG
in Liechtenstein. After that, I decided to change the type of thinning
abrasives from diamond spray on a soft cloth to SBT diamond abrasive films
with fixed diamonds (30µm, 15µm, 6µm, 3µm, 1µm and 0.5µm grain sizes),
finally followed by colloidal silica on a soft cloth.

However, due to the usefulness of retaining the stabilizing collar of
capsule tube, I still want to use the Gatan Disk Grinder (GDG) for parallel
thinning, instead of the otherwise impressive SBT Tri-pod polisher
demonstrated at the course. Regarding the glue for fixing the specimen on
the specimen mount of the GDG, I have changed from wax to Crystalbond 509,
in order to avoid remaining films after cleaning in aceton. Finally, I
intend to perform low-angle ion beam milling using the BAL-TEC RES 010,
possibly preceeded by dimple grinding.


Based on this, I would like your comments on the following questions:

-What are the experiences from the combination Gatan Disc Grinder and South
Bay Technology (or 3M Imperial) diamond abrasive films (8´´, plain back),
adhering by capillary forces to a slowly (50-100rpm) rotating glass plate?

-Is it always (or exclusively for ductile materials) necessary to use all
of the above grain size steps and/or remove a damage layer from the
previous step corresponding up to three times the grinding grain size? (If
so, given a cutting wheel such as Struers 330CA having 68µm (Grit #220)
diamonds, it seems necessary to cut samples thicker than 400µm!)

-Is it necessary/unsuitable to press the GDG against the diamond abrasive
film, or is the weight of the GDG itself (660g) sufficient? (If the
specimen area would carry the total weight, it would correspond to a
pressure of approx. 1MPa (135psi).

-Is it beneficial to rotate the GDG itself during polishing?

-Is it beneficial to grind down the specimen continously or incrementally
(50µm steps are recommended by Gatan). If stepwise, should the steps be
related to the diamond film grain size?

-Is the fit between the specimen mount and the GDG itself crucial for the
integrity of the glued specimen? Which tolerances (after wear) can be
tolerated?


Thanks in advance,

/Richard
________________________________________________________________________
Dr. Richard Larker, Ph.D. Phone: +46 920 91107
Research fellow Fax: +46 920 99309
Division of Engineering Materials
Lulea University of Technology E-mail: Richard.Larker-at-mb.luth.se
S-97187 Lulea, SWEDEN
________________________________________________________________________







From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Mon, 5 Feb 1996 16:02:50 -0600
Subject: Textbook(s)

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I am going to be teaching a mixed TEM/SEM course to undergraduates
in Materials Science next quarter. I am sending this note out to ask
for comments/suggestions about textbooks, both from people who have taught
and those who have been at the recieving end.

NOTE: For obvious reasons, please respond directly to me rather than
everyone on the listserver unless you really intend to do the latter.





From: elaine.levy-at-well.ox.ac.uk (Elaine Levy)
Date: Tue, 06 Feb 1996 09:20:56 +0000
Subject: subscribe

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Please would someone send me the list serv address for subscribing to this list.

Thank you

Elaine Levy
--------------------------------------------------------------
Elaine Levy PhD
Cytogenetics Laboratory
Wellcome Trust Centre For Human Genetics
Windmill Rd.,
Oxford OX3 7BN

tel 01865 740022
fax 01865 742186
email elaine.levy-at-well.ox.ac.uk
-----------------------------------------------------------------





From: CSEDAX-at-ARCRIDE.EDU.AR
Date: Tue, 6 Feb 1996 08:08 -0300
Subject: Need information about CIT ALCATEL difussion pumps oil

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Hi everyone,

our group has received 2 bottles of CIT ALCATEL 220
diffusion pumps oil for free. We have no information at all about how good
it would be for our JEOL SEM and we have found no cataloges around. If any of
you knows something about vapor presion of this oil, or has experience using
it, and has a little time for writing to us, we would appreciate it very
much. Thanks in advance,


Silvia Montoro
csedax-at-arcride.edu.ar

Regional Center for Research and Development
Santa Fe
Argentina





From: Stephan Helfer :      S.Helfer-at-rbge.org.uk
Date: Tue, 6 Feb 1996 09:48:44 BST
Subject: Re: Ethics

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Bill Oliver wrote:

} ... recognizing and accepting individual responsibility
} for individual ethical decisions ...

To me this seems to be the key; together with a well estabished line
of accountability (very important). We will still make mistakes, but
hopefully won't be alone in them...

Stephan




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Tue, 06 Feb 1996 07:08:50 EST
Subject: Comments of Chuck Butterick

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

I would like to set the record straight about "militancy against
academics among certain individuals at SPI" and also, the record with
regard to the true complexion of those at NSF who were resonsibile for
the drafting and implimentation of NSF Important Notice 91.

First, there is no such "militancy", indeed our firm relies on the
graduates of the nation's academic institutions just like any other
employer. Our future business prospects in fact are closely
intertwined with the future prospects of our academic customers. So
any suggestion to the contrary, as they say on the other side of the
ocean, is just pure rubbish.

If there is indeed any "militancy" at all, it is on the part of a small
handfull of individuals and departments who are literally running
businesses out of their university laboratories and who feel threatened
should anyone suggest that what they are doing is not right.

So surely there is concern about anyone using university equipment in
competition against private sector laboratories such as our own. For
the most part, people I know in academia are on "our" side of this
issue and hold the view that the university's instrumentation in fact
should not be used this way. They seem to feel that those projects that
are basic and fundamental in nature and suitable for inclusion in a
student's thesis (e.g. those projects that do contribute to educational
objectives) take up the available time for their instrumentation anyhow
and that outside commercial work just gets in the way and interferes
with the progress of the students and the quality of the work being
done. So those who advocate some contrary view are clearly on the
wrong side of the tracks on this issue, and are also on the wrong side
with respect to their own academic peers.

Second, at the time of the drafting of NSF Important Notice 91, the
Director of NSF was Prof. George Pimentel, perhaps one of the most
respected and published academicians ever to head NSF. He came to NSF
from the Chemistry Department at the University of California-Berkeley.
He was an "academic" and represented "academic interests" in every
sense of the word. To suggest otherwise is to misrepresent Dr.
Pimentel's attitudes, perspectives, and outlook.

The Associate Direrctor at the time was Dr. Donald Langenberg. Prior
to his position at NSF he was a full professor of physics (at the U. of
Pennsylvania, if I remember correctly), and after his tenure at NSF
moved on to serve as President of the American Association for the
Advancement of Science, the Chancellor (I think that was the postion)
of the University of Illinois - Chicago and from there to his present
position of Chancellor of the University of Maryland system, with over
sight over all state universities in the State of Maryland.

Both Drs. Pimentel and Langenberg were in attendence at most of the
meetings that led up to the drafting of NSF Important Notice 91. And
their participation in the drafting of the document was certainly in no
way to be thought of as being passive. So for anyone to suggest that
in some way the academic community was not properly represented is
again "pure rubbish".

But intentional or not, it is obvious that there are still some who
would try to discredit the NSF Important Notice 91 document in every
possible way, in this case, by suggesting there was a lack of academic
representation among those involved with its drafting and
implimentation.

With regard to some of Chuck Butterick's other comments, unethical
behavior of any type should not be tolerated in any environment where
high quality research is being conducted. Vendors guilty of the kinds
of infractions described in Chuck's posting should be reported to the
appropriate procurement officials who have in place mechanisms by which
such abuses of process can be rooted-out and stopped. Of course it
does take two to tango and therefore the other side of the equation,
that is, the customer who is encouraging such inappropriate practices
should also be reported. While the wheel of "justice" might move
slowly, the wheel does move, but please don't paint the behavior of the
entire vendor community with the same brush. The electron optics
industry of manufacturers and distributors by and large is highly
professional and honest and when instances do occur that do not fit
this pattern, there is a process by which one can keep that kind of
circumstance from recurring in the future.

As a final comment, for anyone not personally familiar with NSF
Important Notice 91, I would be happy to FAX you a copy, just send me
your FAX number.


Chuck (Garber)
======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================







From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Tue, 06 Feb 1996 10:56:47 -0600
Subject: Re: static

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At 08:21 AM 2/6/96 EST, you wrote:
} Joiner-
} If you *do* find an old Zerostat gun (which is a little like searching for
} brontosaurus chow), it's probably not going to be any good any more. The same
} effect can be acheived by using glow discharge. Have you got that on an old
} evaporator or coater? Good luck!
} Steven
}
}
**************
Yes, I do; although the evaporator ain't no spring chicken.





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Tue, 06 Feb 1996 10:56:45 -0600
Subject: Re: Staining Problems

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At 09:06 AM 2/6/96 -0500, you wrote:
} Good Morning.
}
} Further to the note I sent you last week where I fingered the grids as my
} most likely suspect for the introduction of Pb hexagons:
}
} My understanding is that these things are made by a plating method. We had a
} program here for a while looking at the microstructure of thin film magnetic
} media for one of our then subsidiaries. The magnetic media is deposited on
} plated substrates.
}
} One of the things we struggled with, is that the plating procedure was done
} in baths contained by tanks soldered together with (you guessed it) Pb. The
} Pb impurities found their way into the plated metal (concentrating at the
} surface), and when I would microtome these things, I'd get hexagons aplenty!
}
} Perhaps the source of contamination in the grids?
}


*********************
That has been proposed (the grids being the source) and we are gearing up to
improve our grid washing.

* * Joiner Cartwright, Jr. * *





From: emccjb-at-ttuhsc.edu (Charles J. Butterick)
Date: Tue, 6 Feb 1996 11:16:35 -0600
Subject: Comments of Chuck Garber

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On June 22, 1995, Chuck Garber wrote, "A special committee, made up
of top NSF officials (the late Dr. George Pimentel played a key role in the
formulation of the final document as did also Dr. Donald Langenberg, now
Chancellor of the University of Maryland system), lawyers representing the
NSF (Mr. Charles Herz who is still at NSF in that same capacity), members
of the independent laboratory community (including myself) plus some other
knowledgeable people came up with the first draft of NSF Important Notice
91."

I still stand by my statement, no academic representation existed.
Chuck Garber was once a part of academics, but he doesn't represent
academic interests. NSF scientists, though academics at one time, are
representing NSF and no one else. That committee appears to have been a
stacked deck of cards. This is a democracy. Bad law can be overturned.




Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: VETO-at-BCRSSU.AGR.CA
Date: 06 Feb 1996 14:02:39 -0400 (EDT)
Subject: Re: freeze substitution to retain soluble components

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Dear Shea,

Low temrerature embedding/infiltration is not an easy procedure. The
concetration and the distribution of the soluble proteins play an important
role of successful LT embedding. I have had some success to retain soluble
proteins in different type of plant tissues. Enzyme activity in vacuoles
and in cell wall,using post-embedding techniques is our interest. Lowicryl
resins: HM23 and K4M, as well as LR White were used at different temperatures
and times (-80 to -20C; 2days to 2 weeks) after "mild" GA fixation OR cryo-
fixed plant tissues, using PLT techniques (dehydration at progressively
lower temperatures). LR White, after UV polymerization developed very small
air (?) "packets". It doesn't section happily, yet not a major problem.
"An Osmium-free Method of Epon Embedment That Preserves both Ultrastructure
and Antigenicity for Post-embedding Immunocytochemistry", Phen, K.D.,
Rustioni.A., and Weinberg, R.J., J. Histochem.Cytochem. 42, 1995. You may
adopt this procedure to LR White. It should work well. Good luck!
Any other ideas, please let me know.
Regards,

Laszlo J. Veto
Electron Microscopist
Agriculture and Agri-Food Canada
Summerland, BC
Ph: 604-494-7711
Fax: 604-494-0755
e-Mail: veto-at-bcrssu.agr.ca




From: John Menzies :      jmenzies-at-spartan.ac.brocku.ca
Date: Tue, 6 Feb 1996 14:57:54 +0001 (EST)
Subject: Re: freeze substitution to retain soluble components

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Please Unsubscribe me.

Thanks






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Tue, 06 Feb 1996 10:56:48 -0600
Subject: Re: Staining Problems & static

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} Robyn -
}
} I will indeed share what I've gleened from this thread, but as I told Dwight
} Beebe, not until you all have solved my problem. No, actually I will pass it
} on. That's what this listserver is for. The static charge sometimes found on
} grids has been suggested to be causing precipitation of the stain. If I can
} find one of those antistatic guns that we used to de-charge our LP's with,
} I'll try it, being the student of reason and logic that I am and staunch
} supporter of the scientific meathod. Then at the next full moon I'm going to
} nail a dead cat to the north side of an oak tree....
}
} * * Joiner Cartwright, Jr. * *

Barring a Zero-Stat gun, which I've still seen advertised
somewhere, try touching your charged grid to a bit a metal on a grounded
piece of electrical equipment. It's safe (this is how some powerbars drain
your personal static charge so you don't zap your computer).
Phil

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu








From: Liang, Long :      LLIANG-at-is.arco.com
Date: 06 Feb 1996 15:45:15 CST
Subject: SEM/cathodoluminescence

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Message-Id: {MACMS.LLIANG.653140150096037FMACMS-at-IS.ARCO.COM}

Dear Microscopists,

I received a used ISI DS-130 SEM equipped with a cathodoluminescence
(CL) detector. This detector has no ellipsoidal mirror coupling to it.
I guess this must be a "diode" detector which can detect CL directly
from the sample.

(1) Does anyone have information about this type of detector ? How does
it compare to the detector with an ellipsoidal mirror ?

(2) Does anyone have experience taking CL images of quartz? I know we
cannot focus the CL, but at least we can use proper settings (by
adjusting accelerating voltage, beam current, etc) to optimize the CL
resolution. Thanks.

Long Liang
ARCO EPMA/SEM Lab
lliang-at-is.arco.com






From: sassaroli-at-msvax.mssm.edu
Date: Tue, 06 Feb 1996 17:46:18 -0500
Subject: Re: Battle of the Chuck's

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} On June 22, 1995, Chuck Garber wrote, "A special committee, made up
} of top NSF officials (the late Dr. George Pimentel played a key role in the
} formulation of the final document as did also Dr. Donald Langenberg, now
} Chancellor of the University of Maryland system), lawyers representing the
} NSF (Mr. Charles Herz who is still at NSF in that same capacity), members
} of the independent laboratory community (including myself) plus some other
} knowledgeable people came up with the first draft of NSF Important Notice
} 91."
}
} I still stand by my statement, no academic representation existed.
} Chuck Garber was once a part of academics, but he doesn't represent
} academic interests. NSF scientists, though academics at one time, are
} representing NSF and no one else. That committee appears to have been a
} stacked deck of cards. This is a democracy. Bad law can be overturned.
}
}
}
}
} Charles J. Butterick (Chuck)
} Electron Microscopy Center
} Department of Cell Biology
} and Biochemistry
} Texas Tech University Health
} Sciences Center
} 3601 4th Street
} Lubbock, Texas 79430
}
} vox (806) 743-1633
} fax (806) 743-1219
} email emccjb-at-ttuhsc.edu or
} chuck-at-micron1.lubb.ttuhsc.edu

Dear Chuck's (Butterick and Garber),

I really believe that this sort of dialog (?!) does not belong here.

Thank you for the excitement, though!!!

Best regards

Massimo Sassaroli, D.Sc.
Dept. of Physiology & Biophysics
Box 1218
Mount Sinai School of Medicine
1 Gustave L. Levy Pl.
New York, NY 10029-6574

sassaroli-at-msvax.mssm.edu







From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Wed, 7 Feb 1996 14:06:49 +1100
Subject: EM: Silver screens for 3D images

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We would like to know if anyone has an opinion on the effectiveness of
using a silver screen and double projectors for 3D imaging. We do not want
to commit ourselves to buying a screen (which is quite expensive) and then
find it has been a waste of money.

We intend to use the system for illustrating talks to members of the the
public visiting the Unit and also for student classes. We would be
projecting mainly SEM images.

We would like to hear from anyone who has tried this or any other
alternative method of displaying 3D images to groups of people.

Thanks in advance,

Mark Gould


Richard Easingwood
South Campus Electron Microscope Unit
Otago Medical School
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD















From: VETO-at-BCRSSU.AGR.CA
Date: 06 Feb 1996 14:02:39 -0400 (EDT)
Subject: RE: freeze substitution to retain soluble components

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From: James S MArtin :      James.S.Martin-at-williams.edu
Date: Tue, 6 Feb 1996 21:55:04 -0500 (EST)
Subject: SEM services near Albany

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I am seeking facilities for SEM-EDS and/or SEM-WDS in proximity to
Berkshire county, Massachusetts (to include western MA, southern VT and
eastern NY). Anyone knowing of a commercial, industrial or academic
facility in this area, please contact me directly.

Thanks.

James Martin




From: Richard.Larker-at-mb.luth.se (Richard Larker)
Date: Tue, 6 Feb 1996 22:48:38 +0100
Subject: More on TEM spec prep for matls science by polishing

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Dear Dr. Hendriks, (and other colleagues interested in the topic related to
the first question (Q1) below),

I have the following comments/questions (C1-6) caused by your answers
(A1-6) to my questions (Q1-6):

Q1: What are the experiences from the combination Gatan Disc Grinder and
SouthBay Technology (or 3M Imperial) diamond abrasive films (8´´, plain
back), adhering by capillary forces to a slowly (50-100rpm) rotating glass
plate?

A1: While I have not used the Gatan tool myself, I can answer you in
terms of using our SBT Polishing Fixtures. Other than the Tripod Polisher
(R) we also have other fixtures which are similar in size and weight to the
Gatan. We have had very good experience in having the films remain firmly
attached - which I presume is your question. We typically recommend that
people use the plain back films as they adhere well, are easy to remove
without damaging them and then can be re-used. This is important as the
films are not inexpensive! You may also want to note that the bottom of
the Gatan fixture is stainless steel (I believe) and it will wear when
polishing with diamond. The SBT fixtures described above have tungsten
carbide feet at the bottom to help resist wear. However, even the tungsten
carbide will wear when polishing diamond. For final polishing on samples
less than a few microns thick, it is best to use a much slower wheel - ie
less than 5 rpm.

Comment 1: In my tests so far, the SBT diamond films were firmly attached
to the wet glass plate by squeezing the water out simply by pressing a PMMA
ruler in several directions on the films. In spite of this, the specimen
(glued to the mount by Crystalbond 509 at 130 C) sticked several times to
the 30 micron diamond film and loosened from the mount, causing expensive
scratches in the film!
I tried to reduce both the incremental steps (from initially 25 micron
down to 5 micron steps) and the rotational speed from 100 rpm to 50 rpm,
but with limited success. I experienced the same problem with the 6 micron
diamond film (but not with the intermediate 15 micron!) when using 2.5
micron steps. The feeling during this "seizure" was that the whole GDG was
sucked (by capillary forces?) firmly to the film, twisting it loose,
causing scratches and loosening of the specimen from the mount. This kind
of experiences did not occur earlier when using diamond spray on soft
cloths! Perhaps I have used a little too high pressure on the GDG, but the
unpleasant tendency for it to "follow" the film was noticeable even without
any extra load. It was even noticeable when trying without any specimen
mount present in the middle of the GDG!
This was peculiar, since the instructions inside the cover of the Gatan
Disc Grinder 623 specify the following: "Polish specimen preferably using
3M Imperial Lapping films". Furthermore, the instructions recommend as
large increments as 50 micron!
Concerning your comment that the WC-Co feet of the SBT fixtures should
wear less than the large SS plate of the GDG does; supposing that is true,
what does it imply for the wear of the soft Delrin feet of the Tripod?
How is it then possible to know that the micrometer is not
"underestimating" the thinning of the specimen?
What is the influence of the specimen/feet area ratio?


Q2: Is it always (or exclusively for ductile materials) necessary to use
all of the above grain size steps and/or remove a damage layer from the
previous step corresponding up to three times the grinding grain size? (If
so, given a cutting wheel such as Struers 330CA having 68 micron (Grit
#220) diamonds, it seems necessary to cut samples thicker than 400 micron!)


A2: The short answer is NO. You do not need to use every grain size. You
do however, need to be mindful of the 3x rule when polishing. I assume
that you are interested in the microstructure of the sample being polished.
You can polish a sample from 500 microns thick down to 5 microns thick by
using only 0.5 micron film. The problem is that it will take you many,
many, many hours and consume many pieces of film. Reason the many steps
are used is to minimize the polishing time. You can obviously remove
material much faster with a 30 micron film than with a 1 micron film, but
you will also cause more damage. The polishing sequence is determined by
1) amount of material to be removed 2) desired final thickness 3) budget
for film 4) level of patience. I do not fully understand your example of
the Struers 330CA so I won't address that here.

C2: The example of the Struers 330CA diamond cutting wheel, which I have
used for slicing my samples up to now, was after some investigation found
to have a diamonds of 68 micron size (Grit #220), and if this causes 3x68
micron of damage on each side, and you follow the 3x rule with
30-15-6-3-1-0.5 micron diamond films, you simply need to cut thicker (} 740
micron!) than I have been doing so far (400 micron) to end up with any
specimen thickness at all! However, may it be the case that the cutting
wheel causes less than the anticipated 3x diamond grain size damage, due to
the low level of normal force against the surface during cutting, when
compared to polishing conditions?
Regarding thinning of ceramics like silicon nitride, which diamond grain
size steps might be omitted ? Can any step be omitted for Ni-base
superalloys?


Q3: Is it necessary/unsuitable to press the GDG against the diamond
abrasive film, or is the weight of the GDG itself (660g) sufficient? (If
the specimen area would carry the total weight, it would correspond to a
pressure of approx. 1MPa (135psi)).

A3: In general, it may be advisable to add some additional pressure
during the rough grinding stages, however, the during final polishing
stages it is best to minimize the load on the sample. Unfortunately, the
Gatan Disc Grinder has no facility which will allow you to reduce the
specimen load below the weight of the fixture. If you have had problems
with samples cracking when thinning at the final stages, excessive load is
probably the problem.

C3: Due to the experiences given in C1 above, I am not confident with
adding any extra pressure at all! Regarding the thickness of the water
film between the GDG and the diamond film, are there any estimations of the
"regimes of lubrication" (hydrodynamic, partial or boundary lubrication, as
related to the diamond grain size) below the GDG or SBT polishing fixtures,
or even below the specimen itself?


Q4: Is it beneficial to rotate the disc grinder itself during polishing?

A4: Yes, for several reasons. It is best to continually introduce new
cutting faces will increase removal efficiency and also reduce preferential
scratching in the surface. However, in some instances, people will
continue polishing in 1 orientation per grit size. This will provide a
scratch pattern in one direction. When changing to the next size film, you
can rotate the sample 90 degrees and see a new scratch pattern develop.
Once the new scratch pattern covers the surface, you know that you have
removed the scratches from the previous grit size - but not the damage!
Remember the 3x rule.

C4: For the GDG, the specimen mount may not be unambigously prevented
from rotating relative to the GDG orientation; is this also the case for
the SBT polishing fixtures that you have described?


Q5: Is it beneficial to grind down the specimen continuously or incrementally?

A5: This is a question which is peculiar to the Gatan Disc Grinder. With
the Gatan Disc Grinder your specimen is mounted to a post which DOES NOT
freely float inside the larger outside ring. Polishing is accomplished by
extending the sample below the surface of the larger outside ring. This
means that the entire weight of you fixture is resting on your sample.
There is a great likelihood that you will damage your sample and/or round
the edges of your sample. Because of this inadequacy, Gatan recommends an
incremental procedure which minimizes the effect, but maximizes the effort!
In more thoughtful designs (such as the South Bay Technology Fixtures
:-)) the sample is mounted to a free floating rod which is gravity fed into
the abrasive surface. The load on the specimen is thus spread across the
entire base of the fixture. Edge rounding is eliminated because the edges
of the sample are protected by the base of the fixture. Using this type of
fixture, the incremental polishing is not necessary.

C5: How are the SBT polishing fixtures designed to stop further thinning
of the specimen after reaching the intended thickness in each step?


Q6: Is the fit between the specimen mount and the disc grinder crucial
for the integrity of the glued specimen? What tolerances can be tolerated?


A6: Think of the disc grinder as a 2 part device 1) the piston with a
sample mounted at the bottom 2) an outside ring with a center hole. The
fit between the piston and the center hole should be very precise. This
precision is increased by making a longer contact area between the 2.
Unfortunately, the Gatan Disc Grinder has a very short contact area which
will magnify any problems. As the Gatan piston/hole are nearly in contact
with the abrasive surface, I would think the chance for abrasive wearing
away the center hole is rather likely. The sample mount is replaceable so
that part is not a big problem. I would think that if you do wear the
center hole uniformly, you could make special sample mounts to correspond
to the new center hole size. Certainly the accuracy in these 2 areas is
crucial - especially when polishing very thin.
I did notice that you are familiar with Tripod Polishing and that you
said you did not want to do that because you wanted to retain the
stabilizing collar. Since you attended Ron Anderson's course, he probably
spoke to you primarily about "wedge polishing". You may like to know that
it is also possible to use the Tripod Polisher (R) for parallel polishing
by mounting the sample in the center of the 3 micrometers. In fact, this
was the way Tripod Polishing originated. The "L" brackets and wedge
polishing techniques were later developments.
You also mentioned that you now use Crystalbond 509 for mounting your
sample to the holder. As a matter of interest, we supply an identical
product called QuickStick 135 which can be purchased in a package of 20
small (3" x .25" x .25") unwrapped sticks under part no. MWH135. An
alternative may be to use "super glue" or any cyanoacrylate. It bonds
quickly and removes completely in acetone. The only problem is that
removing it is somewhat unpredictable. Sometimes it can come off quickly,
other times it may take quite some time. It is also important to be sure
to use fresh super glue. We use super glue for wedge polishing of our TEM
samples. You can pick it up at a local hardware store.
If you would like information on any of our products, you can contact me
directly or you may contact our office in Sweden, Tudor Barnard (snip)

C6"+": How useful is the Tripod for parallel (not wedge) polishing,
compared to the other SBT polishing fixtures that you have described?
Regarding your representative in Sweden, Tudor Barnard, I bought my SBT
diamond films through him, but he was at that time (august ´95) not able to
find a cyanoacrylic glue that was soluble in acetone (but only in
di-methylformamide or tetra-chlormethane, which I consider to be far more
harmful substances!)


Thanks in advance,

/Richard
________________________________________________________________________
Dr. Richard Larker, Ph.D. Phone: +46 920 91107
Research fellow Fax: +46 920 99309
Division of Engineering Materials
Lulea University of Technology E-mail: Richard.Larker-at-mb.luth.se
S-97187 Lulea, SWEDEN
________________________________________________________________________










From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Tue, 6 Feb 1996 23:42:17 -0500 (EST)
Subject: Re: EM: Silver screens for 3D images

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We have used the silver screen method of projecting 3-D slides of all
varieties for over 10 years. We find it by far the best method for
displaying 3-D material to a large group of people. We use separate
projectors with cross polarizers (I have heard of but not seen large
screen projector wich takes a single slide containing two images).The down
side of the procedure is the time it takes to align the two projectors
and the necessity that all slides be exactly aligned in the same way.
Nothing will make an audience sea sick faster than realigning projectors
between stereo-pairs to accomodate different alignments. We sometime
spend an entire day making sure a group of slides is all set up
similarly. For this reason, for showing one or two people our stereo
work we use premounted pairs in small, hand held viewers. However, this is
not nearly as dramatic as a huge image on a screen. Any one who has ever
stood in front of an audience and presented a stereo presentation will
also tell you that the second down side of the method is trying to keep
your composure while looking out at an audience full of people wearing
silly dark glasses in darkened room.

I hope this helps-

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************

On Wed, 7 Feb 1996, Richard Easingwood wrote:

}
} We would like to know if anyone has an opinion on the effectiveness of
} using a silver screen and double projectors for 3D imaging. We do not want
} to commit ourselves to buying a screen (which is quite expensive) and then
} find it has been a waste of money.
}
} We intend to use the system for illustrating talks to members of the the
} public visiting the Unit and also for student classes. We would be
} projecting mainly SEM images.
}
} We would like to hear from anyone who has tried this or any other
} alternative method of displaying 3D images to groups of people.
}
} Thanks in advance,
}
} Mark Gould
}
}
} Richard Easingwood
} South Campus Electron Microscope Unit
} Otago Medical School
} PO Box 913
} Dunedin
} NEW ZEALAND
}
} Telephone: 64-03-479 7301
} Facsimile: 64-03-479 7254
}
} SOUTHERNMOST E.M UNIT IN THE WORLD
}
}
}
}
}
}
}
}
}
}
}
}




From: SIMONE THERESIA BOESCH :      Simone.T.Boesch-at-uibk.ac.at
Date: Wed, 7 Feb 1996 09:22:31 +0200
Subject: unsubscribe

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Please unsubscribe

Thanks, Simone Boesch

+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+-+
Simone T. Boesch {simone.t.boesch-at-uibk.ac.at}
Dept. of Zoology, University of Innsbruck
Technikerstrasse 25, A - 6020 Innsbruck, Austria
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From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Wed, 07 Feb 1996 09:48:12 +0000
Subject: EM: Silver screens for 3D images -Reply

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Message-Id: {s1188232.025-at-wpo.nerc.ac.uk}
X-Mailer: Novell GroupWise 4.1

Dear Richard

A simple way of projecting stereo images is to get a friendly photographer to
double expose your stereo pair through red and green filters onto slide film.
All you need then is to hand out the old red green glasses and appeal for
them to be returned! Its cheaper that way!

The other main item that you need to know is that there is a convention as
to which way around the exposures are made so as to register re. the red
and green and whch way around the glasses are held.

Keith Ryan
Plymouth Marine Lab.
Citadel Hill
Plymouth PL1 2PB, England





From: Richard.Larker-at-mb.luth.se (Richard Larker)
Date: Wed, 7 Feb 1996 12:20:19 +0100
Subject: Is Gatan active on the net?

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Message-Id: {199602071118.AA23745-at-ursus.mb.luth.se}
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Content-Transfer-Encoding: binary

Does anybody know if Gatan has a WWW-site with FAQ´s on the net, or even
just an E-mail address?

Thanks in advance,

/Richard
________________________________________________________________________
Dr. Richard Larker, Ph.D. Phone: +46 920 91107
Research fellow Fax: +46 920 99309
Division of Engineering Materials
Lulea University of Technology E-mail: Richard.Larker-at-mb.luth.se
S-97187 Lulea, SWEDEN
________________________________________________________________________






From: Manoj.Misra-at-urlus.sprint.com
Date: Wed, 7 Feb 1996 08:36:00 -0500
Subject: Uranyl Acetate

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We are encountering problems in disposal of uranium salts as our site
safety officer considers it as a mixed/radioactive waste. I wonder
what is the experience of other microscopists regarding
radioactivity/disposal of uranium compounds.


Manoj MISRA
Unilever Research US
Edgewater NJ




From: Ker{nen Jaakko-Tuomas :      jaakko-at-butler.cc.tut.fi
Date: Wed, 7 Feb 1996 16:49:00 +0200
Subject: Re: Receiving MicroWorld News e-mail newsletter

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From: Ker{nen Jaakko-Tuomas :      jaakko-at-butler.cc.tut.fi
Date: Wed, 7 Feb 1996 16:51:15 +0200
Subject: Re: Receiving MicroWorld News e-mail newsletter

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Yes, please. I would like receive MicroWord News e-mail newsletter.

jaakko-at-butler.cc.tut.fi




From: geosclmr-at-showme.missouri.edu (Lou Ross)
Date: Wed, 7 Feb 1996 09:10:10 -0600
Subject: X-sectioning of filter paper

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A student in Chemical Engineering wants to examine pore membranes in the
SEM. Currently we are looking at the membrane surfaces but he was wondering
about viewing the membranes in cross-section. Does anyone know a method to
prepare such a section? Please respond to his email address at the
University of Missouri at c517837-at-showme.missouri.edu or you can contact me
directly.

Thanks in advance.

Lou Ross

101 Geological Sciences Bldg.
University of Missouri
Columbia, MO 65211
(314) 882-4777, 882=5458 fax






From: ppons-at-cmefcm.uncor.edu (Patricia Pons)
Date: Wed, 07 Feb 1996 12:16:37 -0500
Subject: Jeol 100B manual

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We have recived a JEOL 100B electron microscope (Series 155037-30) as a gift
from University of Pennsylvania. Although this microscope is in good
conditions, it needs complete rehabilitacion. We have highly qualified
personnel to do this job but we found that the instruction manual and
circuits diagrams we have recived, do not much with the specifications of
the microscope.
If somebody has these manuals from JEOL 100B electron microscope (with the
series number higher than ours) which is not longer useful, we can do good
use of them in this part of the third world.

Dra Patricia Pons
Centro de Microscopia Electronica
Universidad Nacional de Cordoba
Cordoba - Argentina





From: huffe-at-carbon.chem.nyu.edu (Edward J. Huff)
Date: Wed, 7 Feb 1996 12:08:01 -0500
Subject: Re: Is Gatan active on the net?

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} From: Richard.Larker-at-mb.luth.se (Richard Larker)
} Subject: Is Gatan active on the net?

In Netscape navagator, I clicked on "net search"

http://home.netscape.com/home/internet-search.html

waited a moment, typed "gatan" into the search field,
pressed return, and immediately received

http://guide-p.infoseek.com/WW/NS/Titles?qt=gatan&col=WW

which also had a "similar pages" button:

http://guide-p.infoseek.com/WW/NS/frames/Titles?qt=gatan&rel=278189&col=WW&st=0

The text under "Gatan Inc" points to

http://www.macfaq.com/vendor/software/1449.html

and also gives a home page and e-mail address.

http://www.gatan.com/

I would say that it is not necessary to ask mailing lists
or Usenet news groups for www addresses. Use net search.






From: Terri_Mengelt-R26202-at-email.sps.mot.com
Date: 7 Feb 96 07:54:00 -0600
Subject: TMAH for Back Metal Etch

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Fellow Microscopists:

Does anyone have information on TMAH as a back-etch for silicon devices? I have
one paper "A New Back-Etch for Silicon Devices" P. Malberti, M. Ciappa , P.
Scacco. Also, in this paper it refers to a "bain marie". Can anyone tell me
exactly what it is? I am assuming it is like a double boiler.


Terri Mengelt
Motorola COM 1
Phoenix, Arizona 85201

phone: (602) 244-4914
fax: (602) 244-6492

email: R26202-at-email.sps.mot.com




From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Wed, 7 Feb 1996 10:00:54 -0600
Subject: Textbook(s) - Follow up

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I would like to thank everyone who responded to my earlier email
(see the end). A number of you were interested in the responses, which I
am including here (with editorial comments omitted). As a follow up, I
heard almost exclusively from faculty and only one student - how about
a few more comments from the students (I will preseverse anonimity).

This is a list of the responses:

4 Responses suggesting (but SEM only)
"Scanning Electron Microscopy and X-Ray Microanalysis", by Goldstein, et.al.,
Plenum Press

3 Responses suggesting
"Electron microscopy and analysis"
P.J.Goodhew and F.J.Humphries, Taylor and Francis 1988.

"Light and Electron Microscopy" by Slaytor and Slaytor.

"Scanning Electron Microscopy and X-Ray Microanalysis" by Robert E. Lee

"Scanning and Transmission Electron Microscopy,"
by Flegler, Heckman and Klomparens (W.H. Freeman and Co., 1993)

"Electron Beam Analysis of Materials" by Mike Loretto

---- Copy of Original Message -----

I am going to be teaching a mixed TEM/SEM course to undergraduates
in Materials Science next quarter. I am sending this note out to ask
for comments/suggestions about textbooks, both from people who have taught
and those who have been at the recieving end.

NOTE: For obvious reasons, please respond directly to me rather than
everyone on the listserver unless you really intend to do the latter.




From: zaluzec-at-microscopy.com (Nestor J. Zaluzec)
Date: Wed, 7 Feb 1996 16:31:59 -0500
Subject: Minor Problems

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G'day Subscribers...

We've got a minor glitch in the subscribe/unsubscribe system.
Unfortunately, I'm on vacation and too far away to fix the link.
This just means there will be a delay if your trying to
unsubscribe. I'll be logging in remotely and doing manual
updates, but the connections are sometimes slow from here.

I'll be back in town the middle of next week and we should
be back to normal.

Sorry for the inconvenience

Nestor
Your Friendly Neighborhood SysOp

Weather report: 34 C, partly cloudy, and the view on
the beach is great. ;-)






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Wed, 07 Feb 1996 10:59:52 -0600
Subject: Re: Battle of the Chuck's

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At 05:46 PM 2/6/96 -0500, you wrote:

} Dear Chuck's (Butterick and Garber),
}
} I really believe that this sort of dialog (?!) does not belong here.
}
} Thank you for the excitement, though!!!
}
} Best regards
}
} Massimo Sassaroli, D.Sc.
}
********************************

Massimo -

At the risk of adding UNNECESSARY fuel to the fire, I'm going to disagree
with you. These are issues that are indeed important, and if these two
gentlemen (and they ARE gentlemen) can hash them out without resorting to
nastiness, we will all benefit. It is easy for us academics to overlook the
position of the commercial/for profit operator, and I assume that the
converse is also true.

I would point out that there may be more than two sides to the question, or
at least an intermediate position. My lab, for example, is in a medical
school and I work WITH investigators who pay me for my services with their
grant money. My lab receives no direct grant funding, either in its capital
set-up and improvements or its operation. The only financial support we get
is what we earn in fee-for-service.


Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Wed, 7 Feb 1996 17:19:51 -0600
Subject: Seeks Position

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I am a microscopist with 21 years experience in Scanning Electron Microscopy in
materials science. I am seeking a new position as a lab supervisor or a lab
support technician. If you have or know of a position available that would
utilize these basic qualifications, and to receive a copy of my resume, please
contact me:

Ms. Kathy Vulu (612)521-2049





From: zaluzec-at-microscopy.com (Nestor J. Zaluzec)
Date: Wed, 7 Feb 1996 16:34:11 -0500
Subject: Minor Problems

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Message-Id: {199602071416.IAA23407-at-Sparc5.Microscopy.Com}

G'day Subscribers...

We've got a minor glitch in the subscribe/unsubscribe system.
Unfortunately, I'm on vacation and too far away to fix the link.
This just means there will be a delay if your trying to
unsubscribe. I'll be logging in remotely and doing manual
updates, but the connections are sometimes slow from here.

I'll be back in town the middle of next week and we should
be back to normal.

Sorry for the inconvenience

Nestor
Your Friendly Neighborhood SysOp

Weather report: 34 C, partly cloudy, and the view on
the beach is great. ;-)






From: slc6-at-lehigh.edu (Sharon Coe)
Date: Thu, 8 Feb 1996 13:17:37 -0400
Subject: Minor Problems

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Please publish the following information in the MicroWorld e-mail newsletter:


1996 Lehigh Microscopy Short Courses
June 10-21, 1996
SEM, X-ray Microanalysis, AFM and SPM, AEM
Contact: Sharon L. Coe
Department of Materials Science & Engineering
Whitaker Labaoratory
5 East Packer Avenue
Bethlehem, PA 18015
610/758-5133 (phone)
610/758-4244 (fax)
slc6-at-lehigh.edu
http://www.lehigh.edu/~inmatsci/Microscourses.html




Thanks you for your help.

Sharon L. Coe
Department of Materials Science & Engineering
5 East Packer Avenue
Lehigh University
Bethlehem, PA 18015
610/758-5133
e-mail: slc6-at-lehigh.edu






From: colijn-at-kcgl1.eng.ohio-state.edu (Henk Colijn)
Date: Wed, 07 Feb 1996 09:03:53 -0500 (EST)
Subject: Re: EM: Silver screens for 3D images

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I have projected stereo images successfully by creating red/green anaglyphs
on transparency material. We took our digital images, combined them using
PhotoShop or similar program, and printed them on a dye-sublimation
printer. Using a standard overhead projector and the red/green glasses,
the images greatly impressed my audience. The dual projector system seems
a bit specialized.

Henk

} We would like to know if anyone has an opinion on the effectiveness of
} using a silver screen and double projectors for 3D imaging. We do not want
} to commit ourselves to buying a screen (which is quite expensive) and then
} find it has been a waste of money.
}
} We intend to use the system for illustrating talks to members of the the
} public visiting the Unit and also for student classes. We would be
} projecting mainly SEM images.
}
} We would like to hear from anyone who has tried this or any other
} alternative method of displaying 3D images to groups of people.
}
} Thanks in advance,
}
} Mark Gould
}
}
} Richard Easingwood
} South Campus Electron Microscope Unit
} Otago Medical School
} PO Box 913
} Dunedin
} NEW ZEALAND
}
} Telephone: 64-03-479 7301
} Facsimile: 64-03-479 7254
}
} SOUTHERNMOST E.M UNIT IN THE WORLD
}
}
}
}
}
}
}
}
}

Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility
-------------------------------------------------------------------
An optimist believes that we live in the best of all possible worlds.
A pessimist fears this is true.






From: jbpawley-at-facstaff.wisc.edu (Jim Pawley)
Date: Wed, 7 Feb 1996 18:33:38 -0600
Subject: Re: EM: Silver screens for 3D images

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} I have projected stereo images successfully by creating red/green anaglyphs
} on transparency material. We took our digital images, combined them using
} PhotoShop or similar program, and printed them on a dye-sublimation
} printer. Using a standard overhead projector and the red/green glasses,
} the images greatly impressed my audience. The dual projector system seems
} a bit specialized.
}
} Henk

The Anaglyph system is indeed simple and easy to use and requires only an
ordinary projector and screen but it does have at least two snags. Apart
from the opportunities that it gives to "Murphy" to attack when two
exposures must be recorded on the same piece of film in register and
through different colored filters, many people get very uncomfortable when
the color of the image presented to one eye is very different from that
presented to the other. The phenomenon is referred to as "color
bombardment" and was much discussed by Vernon Barber in the late
seventies/early eighties with reference to Scanning Electron Microscopy.

The second snag is that you cannot show colored images using this system.
For instance you cannot use it to show stereo views of confocal data in
which you have superimposed separate 3D images obtained from 2 or 3
different fluorescent dyes in the same specimen. Likewise, you cannot show
stereo SEM images in which the SE signal is recorded in tones of grey while
the BSE image is superimposed in shades of red.

In addition, although there is usually less overlap between the red and
blue filters than between the red and the green, the light output in the
blue from most projectors is insufficient to convey many grey levels to the
screen and back.

Once you accept the difficulty of finding an "aluminized" screen, the Pol
system has much to recommend it.

Jim Pawley

****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU






From: jerry-at-biochem.dental.upenn.edu
Date: Wed, 7 Feb 1996 10:43:19 -0500
Subject: Re: EM: Silver screens for 3D images

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Hello Mark Gould and Richard Easingwood,

I have done just as you suggest. Using a portable silver lenticular
(sp?) screen (~$200.00) which does not depolarize the images, a beautiful 3D
image slide show can be projected.

In our department, we have had the capability of mixing 2D and 3D images in
a single slide presentation for about 5 years now and I have even done a few
3D demonstrations at a local high school -- with great success (held their
attention from start to finish)!

As you have outlined, two matched projectors are required as well as
polaroid transparancy sheets and glasses. Using a single piece of Scotch
Tape to attach (hang) a piece of the polaroid transparancy over the front of
the objective lens of each slide projector (each piece oriented 90 degrees
to the other) is sufficient to project a polarized image on the screen.

The glasses, which each person viewing the projected images must have,
represents a bit of work. I made a template for the eyes (no ear
supports/hangers needed - see below dowell sticks) and traced the template
image for as many glasses as I needed on firm, but flexible, cardboard that
could be cut with a good pair of sharp tipped sissors.

After tracing and cutting the cardboard, using carefully applied small
pieces of Scotch Tape, I taped the polaroid 'lens pieces' from the same
sheet of polaroid material used in front of the projector lenses [CORRECTLY
ORIENTED] for left and right eye. By correctly oriented, I referring to the
necessity of having all glasses with the angular orientation of the polaroid
pieces identical -- all left and right eye orientation must be the same in
the glasses and matched to the orientation of the pieces in front of the
projector lenses. This way, all observers receive the same (correct) image
in the left and right eye.

When the 'lenses' were all taped in place, I then taped these glasses by
their right or left edge (makes no difference) to a 12 inch length of 1/4
inch wooden dowell stick so that viewers could hold the glasses in front of
their eyes (and personal prescription glasses if that's the case) much like
holding those small masks at a masquerade party. This may seem a little
amusing, and sometimes gets a laugh at the beginning of a 3D presentation,
but it was the method I developed to circumvent any health hazard concerns
from the repeated public use these glasses -- this is why there are no ear
supports as on regular glasses.

From the SEM image standpoint, I take two photos of the same subject
(carefully aligned to be nearly the same field) at angles of +/- 5 degrees
off verticle by stage tilts. I can then make direct positive slide
transparancies with B/W 35 mm microfilm for projection. Both images are
matched for size when projected on the screen and simply superimposed -- the
glasses and polaroid pieces do all the separation of images to create a very
clear and satisfactory 3D image for the viewers.

Hope this helps. This note has been a sort of 'stream of thought'
description so please don't hesitate to ask any questions.

Good luck -- Jerry Harrison






From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 7 Feb 1996 13:37:37 -0500 (EST)
Subject: Re: Uranyl Acetate

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} We are encountering problems in disposal of uranium salts as our site
} safety officer considers it as a mixed/radioactive waste. I wonder
} what is the experience of other microscopists regarding
} radioactivity/disposal of uranium compounds.
}
Dear Manoj,
The law in New York allows us to pour uranyl acetate down the regular
sink for the amounts used for staining. For larger amounts, they have speci-
fied procedures, and our responsibility is to give the material to them with
the activity listed on a data sheet--they do the rest.
Yours,
Bill Tivol





From: Scott Holt :      102467.2752-at-CompuServe.COM
Date: 07 Feb 96 18:53:12 EST
Subject: GATAN disc grinder on diamond films

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Dear Dr. Larker,

In response to you questions regarding the use of a GATAN disc grinder on
diamond lapping films:

While I don't mean for this response to be an advertisement for product, I must
preface my remarks by mentioning that the experiences and methods described here
occurred using the products of my employer: BUEHLER, LTD in our development
laboratory. Therefore, I, like Mr. Henriks, do have a commercial interest in
your successful specimen preparation.

A1: In response to your query on experiences with GATAN's disc grinder on
Diamond Lapping Films, I also have not used the GATAN fixture; however, I have
tried (successfully, I might add) to grind metallurgically mounted samples
(1-1/4" in diameter) on BUEHLER's ULTRA-PREP diamond films. The problem of
adhesion between the sample and the film, when water is used as a lubricant,
does cause movement of the diamond film on the glass wheel. Our experiences
indicate that wheel speed makes no difference. However, success was achieved by
using a PSA (Pressure Sensitive Adhesive) backed film, attached to the surface
of a POLIMET Pad. It was determined that the diamond films are extremely flat
compared to comparably sized silicon carbide papers, and therefore a thin film
of water between sample and abrasive film causes the sticking problem. Breaking
up this film of water is the key to stopping the sticking. The POLIMET pad is
perforated, leaving small depressions in spots under the film, while maintaining
the rigidity needed to keep the sample flat. Another option for you is to
break up the smooth, flat surface in contact with the film; i.e. the bottom of
your GATAN fixture (this may not be the more convenient option, however).

With regard to your question about the DELRIN feet of tripodding type
polishers, I have had experience here as well. BUEHLER's TRIPOINT POLISHER(TM)
also uses DELRIN feet. These DO wear when held against the diamond films.
However, the purpose of the TRIPOINT POLISHER(TM) type fixtures was originally
to cross-section microelectronic materials. While the DELRIN does abrade
slowly, it does not abrade anywhere near the rate of silicon, GaAs, etc. The
micrometers on which the feet are mounted are there for the purpose of adjusting
tilt and pitch of the sample, as well as adjusting for the insignificant (when
polishing most materials) wear encountered on the feet.

The influence of the DELRIN foot area is important with regard to the sticking
problem. Obviously, the smaller the feet, the less sticking that will occur.
However, another variable should be considered: The abrasive size. On a 30
micron diamond film, the TRIPOINT POLISHER(TM) does not stick because the
diamond is large enough to allow air to break up the water film between foot and
diamond film. However, when you get in the smaller sizes, e.g. 1 to 0.5
microns, the DELRIN feet become 'polished', and the sticking problem begins to
increase. This is sometimes felt as a steadily growing chattering of sample on
abrasive surface. My suggestion is to cut grooves in the DELRIN feet in order
to break up the large surface area found there.

A2: The 3X rule of abrasive damage removal was proposed by a competing
metallurgical supply company a number of years ago. It is a very good guideline
to work with, but it is not a scientifically proven rule, as far as I am aware.
Of course ductile materials will tend to follow the rule more than hard, tough
materials. Therefore, I don't see any reason why you must use every abrasive
size while grinding your materials. However, for samples where edge retention
is important, or where a cross-section of extremely thin layers is being
attempted, I have found that the prescribed steps of 30, 15, 6, 3, 1 and 0.5
micron diamond films are necessary. Especially the latter case.

If you would like to eliminate a significant portion of the grinding that is
necessary, you may want to try a diamond wafering blade which contains a finer
abrasive than the 68 micron diamond you are currently using. We offer a blade
which contains diamond about 1/13th this size, and which is designed for exactly
this purpose. We call this the L5 series blade. You can contact me directly by
e-mail, phone or fax for more info. The surface finish produced is close to
that produced by a 3 micron abrasive film. I do have a BUEHLER produced,
technical brochure (Note: contains info on only our products) which describes
the effects of cutting force, speed, abrasive size, etc., if you would be
interested.

A3: Our experience has been that low loads give better results on diamond
lapping films than high loads. This is especially true with ductile materials.
Cleaning the films with a fresh, folded paper towel during the grinding step
also improves the result obtained on most materials. The towel captures loose
diamond and swarf before it can be reintroduced to the sample; causing scratches
or embedding. Make sure to use a fresh towel with every abrasive size change.

A4: I differ with Mr. Henriks on this question. I don't suggest rotating the
specimen during each grinding step. Rotating the specimen does not introduce
fresh abrasive to the sample. Only the wheel rotation does this.

When the scratch pattern from the previous abrasive is removed, the damage may
or may not be removed (again, depending on the material). However, this may not
be relevant in the case of ceramics such as the silicon nitride you mentioned.
This is because the damage mechanisms are different between ductile and
hard/tough materials.

A5: I do agree with Mr. Henriks on this question. I was, at one time,
Application Engineer at SBT, and have had the opportunity to work with all of
their carbide footed grinding fixtures. The free-floating nature of their
fixtures does reduce the load on the specimen. I believe they still offer a
fixture with spring counterbalancing, which further reduces the specimen load.
These fixtures have an adjustable lock-nut which is dialed to a relative depth.
This nut contacts the piston housing when the piston reaches it's lowest point.
This stops the polishing.

A6: I am not familiar enough with the GATAN fixture to answer this question
fully. However, my experience with using a tripod type device (as Mr. Henriks
suggested) with the sample mounted in the middle of the fixture produces a
sample damaging instability during polishing. Shifting of the fixture from a
base created by the sample and two DELRIN feet to the sample and another set of
feet causes edge chipping if not carefully monitored.

If I can answer any questions for you regarding diamond lapping films,
fixturing, etc., please don't hesitate to contact me directly.

Best regards,

Scott D. Holt
BUEHLER, LTD.
41 Waukegan Rd.
Lake Bluff, IL 60044
Phone: (847)295-4546
Fax: (847)295-7942









From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 07 Feb 96 18:36:13 EST
Subject: Re: TEM-Prep Polishing

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Dear Dr. Larker:

Here are my respones R1-6 to your comments C1-6 from my answers A1-6 to your
questions Q1-6:

Response 1: As you may have read in the response from Scott Walck:

"If you use the diamond films with the GDG, when the sample becomes planar with
the base of the grinder, then the grinder tends to stick to the film by surface
tension because of the large area. This is even more of a problem at the lower
grit sizes."

This will not happen on a soft cloth with diamond spray because you are not
able to form a vacuum as you can with our film. The downside is that you will
end up with rounded edges on your sample. The vacuum problem is exacerbated by
using the small increments which are best to use with the gatan Disc Grinder.
As you polish the sample down or use very small increments, the entire base
becomes nearly co-planar which causes the vacuum to form. On the SBt fixtures,
there is a space between the outside ring and the center piston which minimizes
this contact area and hence the vacuum effect.

I am not surprised that Gatan recommends 3M films, they are not too likely to
refer their customers to SBT! As far as the 50u increments go, that may be an
advisable number to use where it minimizes rocking on the sample caused by the
sample sticking out below the bottom of the fixture, but also may minimize the
vacuum effect. I'm guessing that is the reason.

RE: The Tripod Polisher (R)
The sof t delrin feet of the Tripod Polisher are actually made to wear out. The
idea is that you don't want any material which is harder than your sample to
make contact with the wheel. We use this logic in Tripod Polishing because the
sample becomes very thin and fragile and we do not want any particles from WC-Co
feet to containate the film. Furthermore, the Tripod Polisher (R) has the
facility to adjust for the change in level of the feet. The fixtures with the
tungsten carbide do not have the ability to be adjusted after polishing has
begun so we try to maintain the parallelism as best we can by resisting wear.
The measurements on the micrometer are, for the most part, not used. You make
"relative" adjustments with the micrometers, but you do not actually measure
your sample thickness with them. Sample thickness is typically determined
optically.

The delrin feet on the Tripod Polisher (R) can also create a vacuum if the are
polished flat and co-planar. In fact, we sometimes grind facets onto the feet
to maintain more of a point contact with the film. One of our competitors sells
a tripod Polisher with "non-rotating" micrometers. They charge you more for
them and they are counterproductive. By utilizing non-rotating micrometers you
are creating the very situation you are trying to avoid.

Response 2: If I understand this now, you are cutting your sample with 68u
diamonds which would indicate a damage layer of 3 x 68 or 204u. I suppose you
would need a fairly thick sample if you need to cut on both sides of the sample.
I'm not sure how to avoid this. Of course, this 3x rule is a general rule and
will vary with sample type. Perhaps you would like to do a little study for us
in your spare time?! :} ) Assuming that you are looking for a 1u final thickness
and that you will use each of these steps, the process would then be something
like this:

30u: Polish until 105u thick
15u: Polish until 51u thick
6u: Polish until 21u thick
3u: Polish until 10u thick
1u: Polish until 4u thick
0.5u: Polish until 2u thick
Colloidal silica for final polish.

The rough formula I use is (3 x current particle size) + particle size of next
film in sequence. This gives me the amount of material I need remaining after a
particular step in order to remove thedamage and still have "good" material to
work with. Of course, you may want to be a little more conservative or
aggressive depending on your confidence level. You can remove film steps and
apply the same formula to come up with alternative sequences.

Response 3: You got me on this one. Actually, you probably got me on the other
ones too, but at least I could come up with some reasonable answer for you! : {)

Response 4: No. The SBT polishing fixtures have specimen mounts that are
maintain in a single orientation by way of 2 locating pins that come out of the
center piston and fit into holes in the back of the removable mouting block.
The piston does not rotate because there is a slot in the piston. A set screw
then passes through the outside ring and into the slot to prevent rotation of
the piston relative to the outside ring.

Response 5: We have a micrometer type arrangement at the top of the piston which
allows you to dial in an amount of material to remove. This is done after a
simple zeroing procedure. The dial at the top of the piston will stop the
polishing process at the desired thickness by making physical contact with the
outside ring. This prevents the piston was moving down any further.

Response 6: The Tripod Polisher is extremely effective in polishing very flat
and parralel samples. You have great ability to make adjustments to correct for
any errors in sample mounting and other inaccuracies in the process. However,
since you have the ability to make the changes, you generally need to make them
which makes the process a bit more cumbersome. You can definitely get a great
sample, but you do have to work for it.

Our other fixtures rely more heavily on minimizing the variables. We have a
mounting press of sorts that you use to firmly press your sample against your
mounting block minimizing glue thickness and maximizing parallelism. We also
recommend that you polish your mounting block while mounted in the fixture prior
to mounting your sample. This ensures that your moutning block is parallel to
the plane of the tungsten carbide feet. As there are no adjustments possible,
it is best to follow the entire procedure carefully. Even with no adjustments,
you can get a pretty darn parallel sample! Wealso make a polishing machine
(Model 920) that can be fitted with workstations to hold and rotate the
fixtures. We also build custom attachments (for less than you might think!)
which allow a cusomer to polish 2 sides parallel to each other by simply
flipping the sample over and not re-mounting.

RE: Cyanoacrylate
My understanding is that any cyanoacrylate will be acetone soluble. Perhaps
they just don't list acetone on the label for some reason? A sure bet is to get
something like a nail bonding glue that is used to fix fingernails or attach
fake nails. Check the cosmetic counter. I will try to put a tube of super glue
in the mail to you, but I am not certain if it will still be good when it
arrives. I have tried this before and had problems. That's the reason we
stopped supplying it - it seemed a little silly to make an unhappy customer
because of a $3 bottle of glue that we don't even make!

Well I think I am worn out. You have asked a lot of very good questions and it
is obvious that you take your sample preparation very seriously. I am always
pleased to help in any way i can and i encourage you to contact me whenever i
may be of assistance. Of course, next time i wouldn't mind if talked about your
application for some South bay Technology equipment! : {)

Best regards-

P.S. I would love to get a copy of other responses you get concerning this
topic. Thank you!

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
sbt-at-msa.microscopy.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Wed, 7 Feb 1996 16:51:23 -500
Subject: Sputter Coater Comments?

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At the risk of getting the vendors upset ....


I am getting ready to purchase a replacement sputter coater and I
was hoping to sollicit user comments (either good or bad or general)
regarding either the Pellco SC-7 Automatic sputter coater or the
EMITech K550 automatic sputter coater (aka EMS 550) and/or the
integration of the thickness monitors available for each unit.

PLEASE EMAIL ME DIRECTLY !!!!

ALL COMMENTS WILL BE KEPT PRIVATE !!!!

(I am not looking to publically condemn nor praise either instrument
- I like both companies, but without having worked with either
instrument, I'm looking for comments from those out there who have.)

Thank you in advance.


Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu

int.




From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 7 Feb 1996 13:29:04 -0500 (EST)
Subject: Re: EM: Silver screens for 3D images

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} We would like to know if anyone has an opinion on the effectiveness of
} using a silver screen and double projectors for 3D imaging. We do not want
} to commit ourselves to buying a screen (which is quite expensive) and then
} find it has been a waste of money.
}
} We would like to hear from anyone who has tried this or any other
} alternative method of displaying 3D images to groups of people.

Dear Mark,
We have two large screens--they are aluminum, but look silvery; I
assume these are what you want--for 3D display with polarized light & dual
projectors. The images are quite spectacular and always make a big impres-
sion. If you have many 3D images to display, this kind of system may be
well worth the initial investment. The screens last for a very long time;
ours are } ~25 years old and are as good as ever. I have no idea of the
current price for these screens.
Yours,
Bill Tivol




From: Interface Analysis Centre :      K.R.Hallam-at-bristol.ac.uk
Date: Thu, 8 Feb 1996 08:53:50 GMT
Subject: Uranyl Acetate (fwd)

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We are encountering problems in disposal of uranium salts as our site
safety officer considers it as a mixed/radioactive waste. I wonder
what is the experience of other microscopists regarding
radioactivity/disposal of uranium compounds.


Manoj MISRA
Unilever Research US
Edgewater NJ

Here in Bristol, we are, as long as the University Safety Office knows, and keeps
some control over it, allowed to dispose of up to 100g of uranium per week (I think
it is) in with the normal office/laboratory rubbish. I was surprised when I first found
this out, but it has allowed us to dispose of a collection of unwanted uranium
oxides and the like.
Keith






From: Halldor Gudmundsson :      Halldor.Gudmundsson-at-iti.is
Date: Thu, 8 Feb 1996 09:03:54 GMT
Subject: Looking for SEM-Cryopreparation systems

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I am looking for suppliers of SEM cryopreparation and cryo-transfer systems.
Vendors, suppliers or colleagues with e-mail or fax numbers please respond
directly to me. And yes, I have done the obligatory netsearch with very few
direct scores.

Your sincerely,

Halldor Gudmundsson
Halldor Gudmundsson | Halldor.Gudmundsson-at-iti.is
Project manager |
Technological Institute of Iceland tel: +354 - 587 - 7000
Keldnaholti, IS-112 Reykjavik fax: +354 - 587 - 7409
Iceland





From: cioni-at-dst.unipi.it
Date: Thu, 8 Feb 1996 13:02:50 +0100
Subject: MicroWord News e-mail newsletter

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Yes, please. I would like receive MicroWord News e-mail newsletter.

cioni-at-dst.unipi.it



--
Raffaello Cioni
Dipartimento Scienze della Terra fax 39 50 500675
V. S. Maria 53 phone 39 50 874214
56126 PISA e-mail cioni-at-dst.unipi.it
Italy






From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 08 Feb 96 08:23:48 EST
Subject: Re: re: bain marie

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Terri-
A bain marie *is* a double boiler. It was invented by a medieval Jewish
alchemist known as Mary the Prophetess, hence the name. Aren't you glad you
asked?
Steven Slap
102134,1660-at-compuserve.com





From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Thu, 8 Feb 1996 09:53:33 -0500
Subject: Mechanisms of fluorescence quenching

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Message-Id: {199602081451.JAA18926-at-lihti.org}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Does anyone know a good reference on the possible mechanisms of
fluorescence quenching? Especially processes which involve the interaction
of fluorophores with transition metal complexes or colloidal particles,
from a chemistry perspective (electron transfer, molecular orbitals and so
on).

Thanks,

Rick Powell






From: CSEDAX-at-ARCRIDE.EDU.AR
Date: Thu, 8 Feb 1996 09:22 -0300
Subject: TEM/SEM: particle size distribution - Request

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Hi everyone,

I was wondering whether any of you could help giving me
some tips or references where to look for counting strategy for particles (such
as number of particles to be counted regarding the size and/or magnification).
At this moment I'm trying to obtain polystherene particles size distributions
by TEM and SEM.

Has anyone experience in working with SIGMA SCAN PRO, made by Handel, for
such a distribution? and for images analysis? I would like to hear any comment
about it or about any other software suggested for the subject.

Thanks a lot.
Silvia Montoro
CERIDE - Centre for Research and Development
Santa Fe - Argentina
csedax-at-arcride.edu.ar





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 08 Feb 1996 10:46:45 -0600
Subject: Re: Battle of the Chuck's

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Message-Id: {199602081547.JAA26446-at-watson.bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

At 07:39 PM 2/7/96 -0600, you wrote:

.....Do you have any info, printed or otherwise, regarding your pricing
structure?

***************
No, I don't have anything formalized. I just sat down and calculated what we
were spending to get one case done (costs, including salaries, supplies,
overhead, etc., divided by total number of micrographs, and then how many
micrographs were shot per case). To that I add what I think is reasonable to
support equipment replacement. That's what I charge. For clinical cases the
department adds a professional component.

Joiner





From: WINDLAND-at-odin.ssec.honeywell.com
Date: Thu, 8 Feb 1996 11:53:07 -0600 (CST)
Subject: Polysilicon etch on SOI

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I have a question on etching polysilicon on SOI material. I need to find an
etch that will etch the polysilicon and not the silicon on the buried oxide.
Feel free to call me or send your phone number. Thanks,
Mark Windland
Honeywell
612-954-2845




From: cxb41-at-po.CWRU.Edu (Christine H. Block)
Date: Thu, 8 Feb 1996 12:33:19 -0500
Subject: Affinity

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Message-Id: {199602081733.MAA02220-at-roo.INS.CWRU.Edu}

I am looking for a phone number/ address of Affinity Labs.
who supposedly produce a monoclonal antibody for nitric oxide synthase?

Any information would be appreciated, including other sources for
NOS antibodies for immunohistochemistry.

Thanks!


Christine H. Block, Ph.D.
VA Medical Center
Cleveland OH 44106


--
```
(o o)
*Limbic Lady*-------oOO--(_)--OOo-----




From: images1-at-biosci.mbp.missouri.edu (Michael Stanley)
Date: Thu, 8 Feb 1996 11:19:57 -0600
Subject: 3d images(silver screens)

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Message-Id: {v01510101ad3fdf78c2f4-at-[128.206.15.185]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

We followed the same procedure as Henk (using PhotoShop to produce
red/green anaglyphs from pixel shifted z-sections) but then digitized the
images onto slide film (Kodak Elite) using a Polaroid Palette. A single
projector then did a great job (in fact it actually worked with red/blue
glasses...)


Hank Colijn wrote:
I have projected stereo images successfully by creating red/green anaglyphs
on transparency material. We took our digital images, combined them using
PhotoShop or similar program, and printed them on a dye-sublimation
printer. Using a standard overhead projector and the red/green glasses,
the images greatly impressed my audience. The dual projector system seems
a bit specialized.

Henk


C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia, MO. 65211
(314) 882-4895
fax= 314-882-0123






From: slakmon-at-scottscientific.com (P. Slakmon)
Date: Thu, 8 Feb 1996 15:04:40 -0500
Subject: Automatic LN Refill systems

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Does anyone know of a commercial vendor of an Automatic LN Refill system?


Regards,


Paul Thomson
Thomson Scientific Instruments


_______________________________________________
SCOTT SCIENTIFIC
P.O. Box 66552 Station Cavendish,
Montreal, Quebec, H4W 3J6, Canada

Telephone: 514-485-2309 Fax: 514-485-9931
Voice Mail: 514-888-6509

WWW Site: http://www.ScottScientific.com

E-Mail: slakmon-at-scottscientific.com
info-at-scottscientific.com
sales-at-scottscientific.com
admin-at-scottscientific.com
_________________________________________________





From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Thu, 8 Feb 1996 09:10:05 -0500 (EST)
Subject: Re: EM: Silver screens for 3D images

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A few comments on this thread from our experience:

We have mounted on polarizers on the ends of cardboard tubes. The tubes
are approximately the diameter of the Kodak projector lens barrel. We put
a lenght of the thin packing sponge material that our hazzardous chemicals
come wrapped in around the inside of the tube to provide friction and
hold the tube in place. This allows the polarizers to be rotated to get
maximum extinction with the glasses. We order bulk glasses for a nominal
cost. Most come with the polarizers set at 45 degrees to the horizon.
However, some glasses come with the polarizers horizontal and vertical.
The tube system allows us to adjust or projector polarizers to match
either set of glasses.

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************





From: wise-at-vaxa.cis.uwosh.edu
Date: Thu, 08 Feb 1996 09:12:37 +0000
Subject: Minor Problems

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} Date: Wed, 07 Feb 1996 16:31:59 -0500
} From: zaluzec-at-microscopy.com (Nestor J. Zaluzec)
} Subject: Minor Problems
} X-Sender: zaluzec-at-microscopy.com (Unverified)
} To: Microscopy-at-Sparc5.Microscopy.Com
}
} G'day Subscribers...
}
} We've got a minor glitch in the subscribe/unsubscribe system.
} Unfortunately, I'm on vacation and too far away to fix the link.
} This just means there will be a delay if your trying to
} unsubscribe. I'll be logging in remotely and doing manual
} updates, but the connections are sometimes slow from here.
}
} I'll be back in town the middle of next week and we should
} be back to normal.
}
} Sorry for the inconvenience
}
} Nestor
} Your Friendly Neighborhood SysOp
}
} Weather report: 34 C, partly cloudy, and the view on
} the beach is great. ;-)
}
}


Vacation? VACATION?! That's pretty cheeky. And 34C? Last week we had
MINUS 34 C! Enjoy your break, Nestor.

Bob






From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 8 Feb 1996 08:17:18 -0500
Subject: Regulations galore!

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Message-Id: {n1388354350.95607-at-msmail.tmc.tulane.edu}

After reading a few of the messages on this server from users in different
states representing various regions of the USA, I think about the growing
pains we are facing with what it seems "inconsistent regulation in the UNITED"
deserves a revisit? Two items are worth recollecting: osmium and uranyl
disposal. If you think that the disposal of these chemical (which by all means
are hazardous) think about the follwing. During one my visits to California
(NASA AMES RESEARCH CENTER) I was prohibited (and instructed so formally) from
pouring SALINE (That's right PBS!) down the drain! Thus, I immediately asked
where I should urinate (you know amonia, amino acids, etc). Even though the
biosafety officer did not have a good answer (take that back... regulations)
he was rather upset. The moral of the story: I learn quickly after arriving
to the states long ago, that -the outcome of a procedure matters less than the
implementation of the regulation that requires it-. That is the good news, the
bad one is thatit will get worse if we do not inject a tiny bit of common
sense into all of this mess and others. My two daughters were taught at
school already that animals are terribly mistreated by industry and
researchers, but I had to point out to her the reality behind the production
of a juicy burger and/or taste fried chicken nudget? This was easy for me
because when I was her age I butchered the chickens we ate and witness the
killing and butchering of the pigs, cows, etc. Go on, make your contribution,
and get started now-tell as really is!


*********************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} ----} Prof. Pathology & Otolaryngology *
*http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html*
*********************************************************************






From: Evelyn Clausnitzer :      clausnz-at-itsa.ucsf.edu
Date: Thu, 8 Feb 1996 15:03:54 -0800 (PST)
Subject: TISSUE ADHESIVE

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A visitor to our lab from Switzerland brought us an adhesive used for
sealing wounds, called HISTOACRYL Blue. We have found it to be very
useful and would like to find a supplier in the US. Any leads will be
appreciated.

Evelyn Clausnitzer
U. C. San Francisco




From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Thu, 8 Feb 1996 16:17:30 -0500
Subject: Re: Antibody to Nitric Oxide Synthase

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Message-Id: {199602082115.QAA20410-at-lihti.org}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear Christine H. Block:

This question was asked before, and I replied direct to the poster not to
the listserver. Your supplier for polyclonal nitric oxide synthetase
antibody is:

AFFINITY BIOREAGENTS, INC
14818 West 6th Avenue, #13A
Golden, CO 80401

Tel: (800) 527-4535, (303) 278-4535
Fax: (303) 278-2424
Email: affinity-at-bioreagents.com

In addition, you could check out:

We have just received the new (1996) Lindscott's Directory of Immunological
and Biological reagents (Order from Lindscott's Directory, 4877 Grange
Road, Santa Rosa, CA 95404; phone (707) 544-9555, Fax (415) 389-6025) which
is a good place to start looking for antibodies. Some entries for
anti-nitric oxide synthetase antibodies:

POLYCLONALS:

Rabbit antibody against inducible NOS, mouse macrophage:

Alexis Corp.
P.O. Box 927190, San Diego, CA 92192, USA
Tel: (619) 658-0065, Fax (619) 658-9224

Rabbit antibody against inducible, neuronal or endothelial NOS:

Oxford Biomedical Research
P.O. Box 522, Oxford, MI 48371
Tel (in US) 800-692-4633, Fax (810) 852-4466

MONOCLONALS:

IgG2A against brain NOS, against mac, inducible NOS, against NOS EC:

Transduction Laboratories
133 Venture Crescent #5, Lexington, KY 40510, USA
Tel (606) 259-1550, Fax (606) 259-1413

Against inducible NOS:

Research and Diagnostic antibodies
P.O. Box 8300, Berkeley, CA 94707
Tel (510) 262-9000, Fax (510) 262-9127

Since I've never used these I can't tell you whether they work, but they
are probably good places to start.

Richard D. Powell
*************************************************************
NANOPROBES, Incorporated
25 East Loop Road, Suite 124, Stony Brook, NY 11790-3350, USA
http://www.lihti.org/research/ecodev/incubten/nano/home.html

} } Does anyone in cyberspace know where I can obtain and antibody to Nitric
} } Oxide Synthase which will work on mouse brain.
} }
} } Dr Terry Robertson
} } Electron Microscopist
} } Department of Pathology
} } University of Western Australia
} } Nedlands 6009
} }
} } phone 346 2935
} } Fax 346 2891
} } email troberts-at-eosin.path.uwa.edu.au
}






From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Thu, 8 Feb 1996 18:30:11 -0500
Subject: Re: affinity/antibodies for nitric oxide synthetase

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Message-Id: {199602082328.SAA20856-at-lihti.org}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

(If this appears twice, please ignore the earlier version-my mistake for
E-mailing Microscopy-at-Sparc5 instead of MSA. My apologies)

Dear Christine Block:

Someone else asked about antibodies to nitric oxide synthetase, and I
replied direct to the poster not to the listserver. Affinity Bioreagents
supplies a polyclonal antibody to nitric oxide synthetase; their address
is:

AFFINITY BIOREAGENTS, INC
14818 West 6th Avenue, #13A
Golden, CO 80401

Tel: (800) 527-4535, (303) 278-4535
Fax: (303) 278-2424
Email: affinity-at-bioreagents.com

For other polyclonal and monoclonal antibodies (in reply to the earlier
question):

We have just received the new (1996) Lindscott's Directory of Immunological
and Biological reagents (Order from Lindscott's Directory, 4877 Grange
Road, Santa Rosa, CA 95404; phone (707) 544-9555, Fax (415) 389-6025) which
is a good place to start looking for antibodies. Some entries for
anti-nitric oxide synthetase antibodies:

POLYCLONALS:

Rabbit antibody against inducible NOS, mouse macrophage:

Alexis Corp.
P.O. Box 927190, San Diego, CA 92192, USA
Tel: (619) 658-0065, Fax (619) 658-9224

Rabbit antibody against inducible, neuronal or endothelial NOS:

Oxford Biomedical Research
P.O. Box 522, Oxford, MI 48371
Tel (in US) 800-692-4633, Fax (810) 852-4466

MONOCLONALS:

IgG2A against brain NOS, against mac, inducible NOS, against NOS EC:

Transduction Laboratories
133 Venture Crescent #5, Lexington, KY 40510, USA
Tel (606) 259-1550, Fax (606) 259-1413

Against inducible NOS:

Research and Diagnostic antibodies
P.O. Box 8300, Berkeley, CA 94707
Tel (510) 262-9000, Fax (510) 262-9127

Since I've never used these I can't tell you whether they work, but they
are probably good places to start.

Richard D. Powell
*************************************************************
NANOPROBES, Incorporated
25 East Loop Road, Suite 124, Stony Brook, NY 11790-3350, USA
http://www.lihti.org/research/ecodev/incubten/nano/home.html

} } Does anyone in cyberspace know where I can obtain and antibody to Nitric
} } Oxide Synthase which will work on mouse brain.
} }
} } Dr Terry Robertson
} } Electron Microscopist
} } Department of Pathology
} } University of Western Australia
} } Nedlands 6009
} }
} } phone 346 2935
} } Fax 346 2891
} } email troberts-at-eosin.path.uwa.edu.au
}






From: Ted Boden -at-MR.SEMATECH.Org
Date: Thu, 8 Feb 1996 10:57:00 CST
Subject: op3

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MR-Received: by mta GATEV3; Relayed; Thu, 08 Feb 1996 11:21:30 -0600
Alternate-recipient: prohibited
Disclose-recipients: prohibited


--Boundary (ID D1sGwLyQksj+RO0n0DfJlw)
Content-type: TEXT/PLAIN; CHARSET=US-ASCII



--Boundary (ID D1sGwLyQksj+RO0n0DfJlw)
Content-type: MESSAGE/RFC822



The Materials Analysis group at SEMATECH in Austin, TX has an
immediate opening for a TEM lab Technician. SEMATECH is a
government/industry consortium working on advanced projects related
to the fabrication of 0.25/0.35um CMOS integrated circuits.

For more information please contact Carolyn Gondran -at- 512-356-3149
, Philippe Maillot -at-512-356-3944 or Ted Boden 512-356-3324 or
respond by direct e-mail.



JOB DESCRIPTION:
Sample preparation of semiconductor materials and devices, both
plan view and cross-section, for TEM. Develop improved methods of
sample preparation. TEM operation to facilitate this development
will be encouraged. Photographic processing and filing of TEM
negatives and prints. Maintenance of sample preparation and dark
room equipment and supplies


JOB REQUIREMENTS:
2+ years experience in TEM sample preparation and photographic
processing required. Familiarity with tripod, dimpling and FIB
techniques desired. This position requires strong organizational
skills, and in particular, the ability to work on multiple tasks
while maintaining precise records and tracking procedure.
Experience in microelectronics industry a plus.

Associate degree in physics, chemistry or related subject.

--Boundary (ID D1sGwLyQksj+RO0n0DfJlw)--




From: Ronald Cohn (415) 8556059 :      Ronald.Cohn-at-syntex.com
Date: Thu, 08 Feb 1996 17:25:49 -0800 (PST)
Subject: LM - Detection of apoptotic cells

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Registered-mail-reply-requested-by: Ronald.Cohn-at-SYNTEX.COM
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List subscribers,

We need to identify apoptotic cells in cultures of cells grown in suspension.
We would like to do this using the TUNEL (terminal transferase-mediated
dUTP-biotin nick end labeling) technique with either fluoresence or
enzyme-substrate reaction product as the read-out. I would appreciate hearing
from anyone who has used this technique regarding which commercial labeling
kits may be better than others, recommended protocols, etc. Thanks in advance.

Ron Cohn
Structural Biology Laboratory
Roche Bioscience
Palo Alto, CA
ronald.cohn-at-syntex.com






From: Peter.Stalmans-at-med.kuleuven.ac.be (Peter Stalmans)
Date: Fri, 9 Feb 1996 09:55:27 +0100
Subject: Workshop Confocal Microscopy

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On March 8, 1996, a workshop on confocal microscopy will be held in the
Laboratory of Physiology, KULeuven, Belgium.

Program:

08.30: Registration
09.00: Welcome: Prof. Dr. J. Janssens, Dean of the Faculty of Medicin KU Leuven
09.15: Confocal vs conventional microscopy.
H. van der Voort, J. Bauman, H. Vrolijk, W. Sloos & H. Tanke,
SVI Hilversum, UA & RU Leiden, The Netherlands
09.45: Spontaneously spreading calcium waves in rat cardiac myocytes:
implications of the data acquisition by confocal microscopy.
W. Wussling, Martin Luther University, Halle, Germany
10.15: Coffee and demonstrations on confocal microscopes
11.00: Confocal laser scanning in the reflection mode.
Z. Mischal, CNRS, Villejuif, France
11.30: Free communications
12.00: Lunch
13.15: Demonstrations on confocal microscopes
14.00: Confocal fluorescence life time imaging with two-photon excitation.
H. Gerritsen, J. Sytsma & J. Vroom. Universiteit Utrecht, The Netherlands
14.30: Manipulation, N-dimensional visualisation and measurement of the
living cell with super-resolution and super-contrast.
P. Van Oostveldt, U Gent, Belgium
15.00: Coffee and demonstrations on confocal microscopes
15.45: Free communications
17.00: Final conclusions and farewell. B. Himpens, KU Leuven

Date and location:
The workshop will be held on Friday, March 8th, 1996 at the KULeuven
Lab Physiology, 8th floor
Onderwijs & Navorsing, Gasthuisberg
Herestraat 49
B - 3000 Leuven
Belgium

Contact person:
B. Himpens
Lab Physiology
Herestraat 49
B - 3000 Leuven
tel + 32 16 34 57 27 or + 32 16 34 71 46
fax + 32 16 34 59 91
E-mail: Bernard.Himpens-at-med.kuleuven.ac.be

More information and online registration:
See URL: http://www.kuleuven.ac.be/kuleuven/news/wcm/

Peter Stalmans
Peter.Stalmans-at-med.kuleuven.ac.be
Laboratory of Physiology KULeuven
Herestraat 49
B-3000 Leuven
Belgium
tel: +32-16-34 71 46
fax: +32-16-34 59 91





From: Peter.Stalmans-at-med.kuleuven.ac.be (Peter Stalmans)
Date: Fri, 9 Feb 1996 10:19:07 +0000 (GMT)
Subject: No microscopy - active waste disposal

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Message-Id: {199602091019.KAA14329-at-zeus.bris.ac.uk}

Dear All,
A few of you have expressed surprise at my comment on disposal of active waste here in Bristol. I thought (though there is no direct microcsopy content - shouldn't
this be in one of the safety groups??) you might like a couple of quotes from the relevant University safety handbook.
"Aqueous waste. Our primary waste disposal route is via the sink... This is
both the least restrictive and the least environmentally damaging" It then goes
on about how this would be diluted by all the other waste water the University
discharges into the sewars.
"Very low levels of solid radioactive waste disposal are permitted by out authorisation from HMIP (Her Majesty's Inspectors of Pollution) to the normal waste
bins.""No visible radioactive signs are to be present on the box or bag used for
disposal and please try to ensure that the package does not look interesting..."
There are records that have to be kept, and regulations on there being no
external contamination, maximum permitted levels, only one lot per waste bin, etc.

--
Dr. Keith R. Hallam University of Bristol, Interface Analysis Centre, Oldbury
House, 121, St. Michael's Hill, Bristol, BS2 8BS, England
Telephone: + 44 (0)117 925 5666 | E-mail: k.r.hallam-at-bristol.ac.uk
Facsimile: + 44 (0)117 925 5646 | URL: http://zeus.bris.ac.uk/~phkrh/




From: Neal Nicklaus :      nnicklaus-at-nova.sarnoff.com
Date: Fri, 09 Feb 1996 09:08:36 -0500
Subject: Image Processing Software

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Message-Id: {199602091411.JAA28919-at-vaxserv}
X-Sender: nnicklaus-at-cave.sarnoff.com
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Does anyone know of some "good" PC bases image processing software designed
for microscopy applications? I need to do some time based studies on
fluorescent DNA strands.

I have an optical trap set up to hold and maneuver the strands as attached
to sub micron latex beads. I want to study the effects of various enzymes
and environments on the DNA while in the trap.

Any suggestions as to software and its required (desired) hardware would be
appreciated.






Neal Nicklaus

SEQ Limited

Voice: 609-452-6033 Ext. 13
Fax: 609-452-5955
email nnicklaus-at-seq.sarnoff.com





From: Neal Nicklaus :      nnicklaus-at-nova.sarnoff.com
Date: Fri, 09 Feb 1996 09:15:15 -0500
Subject: UV Microscope Objectives

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Message-Id: {199602091417.JAA28934-at-vaxserv}
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Mime-Version: 1.0
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Does anyone know of custom or commercially available UV microscope
objectives for use in Laser Induced Fluorescence studies?

I have contacted Zeiss, Nikon, Olympus and Leica. Zeiss has the best of the
group but the UltraFluars all fluoresce in my UV laser beam (266 nm). I
want as high an NA as possible, i.e. immersed optics, so the reflective
designs, e.g. Ealing, I have seen are not fast enough.

Fluorescence excitation wavelengths are tunable between 260 and 290 nm.
Fluorescence emissions are from 300 to 500 nm.

Any suggestions would be appreciated. I can afford a custom design after I
prove the concept at more limited wavelengths.





Neal Nicklaus

SEQ Limited

Voice: 609-452-6033 Ext. 13
Fax: 609-452-5955
email nnicklaus-at-seq.sarnoff.com





From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Fri, 9 Feb 1996 09:43:13 -0500
Subject: PolyCutEase or SureCut Plastic additives

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Message-Id: {v01510100ad410824134e-at-[128.206.15.189]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Any comments from cyberspace on the additives that EM supply houses sell to
add to epoxy resins that are supposed to improve sectioning properties? I
am referring to products such as Poly Cut Ease (Polysciences) or SureCut
(EMS). Do people think they actually help? Are there any disadvantages?


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: gbza40-at-udcf.gla.ac.uk
Date: Fri, 9 Feb 1996 13:24:00 GMT
Subject: Aluminised screens for 3D projection

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With regard to using 3D aluminised screens for projecting polarised images ,
I can only add in support of Jim Pawley's comments on the advantages for
imaging colour - in my case cell reconstructions with colour coded
organelles - which come out only with the polarised system.

As regards sourcing a screen try your Chemistry colleagues who probably have
one lurking somewhere as they may project molecular graphics in this way for
teaching. This is where I borrow my portable screen from here in Glasgow !

Laurence Tetley
Dr Laurence Tetley
IBLS EM Centre
Joseph Black Building
University of Glasgow
Glasgow G12 8QQ

email gbza40-at-udcf.gla.ac.uk
tel. 0141 330 4431
fax 0141 307 8016





From: Sheryl K. Brining :      skb-at-helix.nih.gov
Date: Fri, 9 Feb 1996 12:57:40 -0500 (EST)
Subject: Suscribe

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Please send to: skb-at-helix.nih.gov Thanks.





From: bsgphy3-at-uconnvm.uconn.edu (JIM ROMANOW)
Date: Fri, 9 Feb 1996 12:58:09 -0500
Subject: Critical Point Drying

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I am searching for the most comprehensive articles about critical point
drying techniques. All suggestions are appreciated.

Any problems with going directly from ethanol into carbon dioxide or is
amyl acetate useful in between? My specimens look OK using just ethanol.

Also: I am experiencing a run of failing (most often between 400 and 800
psi) front window Dowty seals on my drying unit even if I do only 'dry'
(carbon dioxide only) test runs. Is anyone else having this problem? This
device has been operating for 17 years with few leaks. All sealing surfaces
are smooth and clean.

Thank you in advance.

Jim Romanow
Electron Microscopy Facility
Physiology and Neurobiogy Department
The University Of Connecticut
Storrs
bsgphy3-at-uconnvm.uconn.edu






From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Fri, 9 Feb 1996 11:11:29 -0500 (EST)
Subject: Pol Stereo Glasses

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I have had over a dozen requests for this information so I am posting it
directly to the list:

We have always purchased our stereo glasses from Ted Pella
1-800-237-3526 outside of California
They are in cardboard mounts and relatively inexpensive.
However, I did not see them listed in the current catalog. I hope they
still carry them. Any one know of an alternate supplier?


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************





From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 9 Feb 1996 16:16:10 -0500
Subject: Pol Stereo Glasses

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From: jbpawley-at-facstaff.wisc.edu (Jim Pawley)
Date: Sat, 10 Feb 1996 12:30:41 -0600
Subject: Interested in purchasing cold stage for Philips microscope

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Hello all,

I have prototype LVSEM that employs the stage mechanism from a Philips EM
430 TEM. I would like to purchase a cold stage for this instrument if I
can find one (standard Philips rod). Anyone out there about to
"decommission" one?

Jim Pawley

****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU






From: Glenn Poirier :      glennp-at-eps.mcgill.ca
Date: Sat, 10 Feb 1996 13:14:18 -0500 (EST)
Subject: Re: MULTIPLE USERS ON THE SEM

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Hello Everyone,
I'd just like to put in my two cents on the subject of multiple users of
an SEM (or in my case an electron microprobe)

Our instrument is used at least 16 hours a day and since I'm only paid
for eight almost all our users are trained to use the machine
independantly. This allows them faster access to the machine and saves them
or their advisors money. The only people we don't encourage to become
independant users are those who will only use the machine once or twice.
Most users require 3-4 daytime shifts before i will let them use the
machine independantly (I give them my home phone number too).

In the six years I've been running the lab I can't recall one incident
where the machine has actually been damaged by inexperienced users. On
several occasions it has been temporarily put out of commission (computer
problems, etc) but usually I can get it back on line immediately the next
day. Although there is the potential for a user to damage the instrument
(i.e during a sample change) most of the rest of the instrument is fairly
fool proof. It is easy to screw up your analyses, but difficult to hurt
the machine.

I'm sure the machine would last longer and require less service if I was
the only one operating it, but we don't have that option. I also feel
that users are much better off acquiring their own data, they know what
they want and can change their strategy if they find something unexpected

Hope this is useful.

Glenn





From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Fri, 9 Feb 1996 21:23:55 -0800
Subject: Re: MULTIPLE USERS ON THE SEM

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} I'd like some input, opinions etc. on the pitfalls of having multiple
} operators on an SEM.

Dear Bonnie,
I run a Materials Engineering EM lab at the University of British
Columbia, with two SEMs and a TEM. Most of the researchers who use the
instruments are graduate students and Research Engineers. I have always
encouraged these people to do their own research on the SEMs, as much as
possible, since only they know exactly what they want and that way one me
can keep three instruments optimally used. After I show them how to use the
SEM, I watch and encourage them to ask me for further instruction for
picture taking, higher mag, etc. Some people, who have a lot of work to do,
may gain my permission to use the SEM after hours, but only after I feel
they have gained a lot of experience during working hours, when I am there
to supervise, and only after I have instructed them on how to properly shut
down and what to do in the event of problems. I also purchased the
instruments originally with ease-of-use in mind. They are fully automatic
and easy to get good results on.
I must feel that the person has a good understanding of the important
issues and knows how to properly handle the instrument. Mind you, I don't
have a field emission SEM. I have had damage, but only once in 15 years and
it could have happened in normal hours.. It also helps to scare them, be
"the ogre of the EM lab". Mind you, we are a teaching lab, so the learning
is as important as the doing.
I know many EM operators do not like the idea of letting any ham-fisted
graduate student or engineer loose on their instrument, but modern SEMs
really can be operated by any intelligent being.
So long as you satisfy yourself as to this person's knowledge,
experience and caring, I'd see no harm.
Hope this helps.

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Fri, 09 Feb 1996 15:29:24 -0600
Subject: Re: Re[2]: Staining Problems & static

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Message-Id: {199602092029.OAA28451-at-watson.bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
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At 10:17 AM 2/8/96 EST, you wrote:

".....to form yellow, iridescent hexagonal prisms of lead iodide....."

I stained one grid with our lead stain alone, and another with our uranyl
acetate stain alone. The lead stained grid had the hexagonal deposits and
the other did not. So it's not the uranyl acetate. It's interesting that
lead iodide forms hexagonal crystals.
*******************

".....Perhaps these are your crystals - a bit of a long shot since you did
not mention iodine in our elemental analysis of the crystals...."

No, there is no iodine in either of our stains, or any of the sample
preparation steps; and I did not get an iodine peak with the microprobe.
*******************


I have borrowed an anti static gun like the Zerostatic Eliminator that you
suggest. We are in the process of trying it out. At least the grids don't
jump up and cling to the lid of the plastic petri dish any more.


* * Joiner Cartwright, Jr. * *





From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Fri, 9 Feb 1996 15:15:04 GMT
Subject: Re: PolyCutEase or SureCut Plastic additives

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} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

These things help a great deal when using a glass knife. We use lecithin in
the epoxy accelerator . ( use eual weight of lecithin and accelerator, then
double the volume for use) A glass knife will last a long time if you
have it in the resin. It was a great help to students just learning to
section, They could get usable stuff the first day at the microtome. It
can give a blotchy look to empty resin areas or those that have little
density, but most tissues are "busy " enough that you never notice it. See
Mollenhauer , 1986, J. EM Tech 3:217-222
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: K. M. Anisur Rahman :      anis-at-execpc.com
Date: Fri, 09 Feb 1996 16:13:35 -0800
Subject: Does tin-oxide dissolve in Acetone?

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Message-ID: {311BE32F.7B15-at-execpc.com}

Hi,
Can anybody let me know if SnO2 and Sb2O5 would dissolve in
Acetone at about 100°C? Many thanks,

-Anis.




From: Bonnie Davis - Kennametal Inc. :      bld_kmt-at-prlc.org
Date: Fri, 9 Feb 1996 13:09:08 -0500 (EST)
Subject: MULTIPLE USERS ON THE SEM

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I'd like some input, opinions etc. on the pitfalls of having multiple
operators on an SEM. My situation is that I am part of a materials
analysis group that provides analytical services to corporate
technology. Our group consists of a number of engineers and technicians
with expertise in a variety of analytical areas. Our company has
recently hired a young PhD to do materials research in another department .
He is pushing very hard to be allowed to use our SEM at night and on weekends. He has 'used
an SEM before' and doesn't see a problem in using ours. It has always
been our practice to encourage researchers to come into the lab while
their samples are being analyzed, but not to allow individuals to use the
instrument themselves. I believe that the analysis is done more effectively
by individuals whose expertise is in that area. However, management
appears to be leaning towards changing the rules for this individual, and
having me train him on the instrument.

We have a four year old JEOL 6400 instrument with a Link eXL analyzer,
UTW detector, complete stage automation. We also have a computer system
attached for collecting digital images, we do not use polaroid film.
While this individual has used and SEM he has not used either a JEOL or
LINK system before. I feel that training would be extensive. This is currently the only SEM we have. It
is used approximately 12 hours per day by myself and my staff. Quite
often EDS analysis is carried out using computer control and stage
automation overnight. However, management feels that if someone is
willing to utilize the instrument in those few hours a week when it is
not being used, they need to take advantage of that to receive a maximum
benefit from the asset.

Several years ago we had two older SEMS and due to 'corporate
downsizing', we had only one operator. At them time I trained
approximately 2 dozen engineers who desired to use the 'extra' SEM on
their own. We disposed of the instrument about a year ago when it had
not been touched for almost a year. The time I spent in training
all of those individuals was probably greater than the time they as
a group spent using the instrument. Hindsight being perfect I wish we had
kept the old instrument!

Anyway, I would appreciate any input both pro and con from the list
regarding opening up an instrument to multiple infrequent users.




From: James S MArtin :      James.S.Martin-at-williams.edu
Date: Fri, 9 Feb 1996 17:53:36 -0500 (EST)
Subject: Thanks for SEM-EDS info

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Thank you to all who provided information on SEM-EDS services in and
around western Massachusetts.

James Martin




From: AMCGroup2-at-aol.com
Date: Sun, 11 Feb 1996 20:41:36 -0500
Subject: Microscopy Online's URL

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As a correction to my message of 02-04, please note that the URL for the
Microscopy Online Web-journal is:

http://www.Microscopy-Online.com/

Please accept my apologies.

Rene E. Nicholas
Spec. Prep. Workshop Coordinator
AMC Group




From: AMCGroup2-at-aol.com
Date: Sun, 11 Feb 1996 20:33:11 -0500
Subject: Re: R. Larker's Spec. Prep. Ques

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For your information, the following two companies also sell the
“wedge-polishing” tool, supplies, and various consumables (diamond lapping
films/suspensions, mounting wax/glues, etc.) for TEM specimen preparation of
materials:

UltraMetrix
4802 E. Ray Rd., No. 23-267
Phoenix, AZ 85044
(602) 706-5745
FAX (602) 496-6505
e-Mail ULTMTRX-at-aol.com

Allied High-Tech Products
P.O. Box 4608
2376 E. Pacifica Place
Rancho Dominguez, CA 90220
(310) 635-2466
FAX (310) 762-6808

Rene E. Nicholas
Spec. Prep. Workshop Coordinator
AMC Group




From: rw9-at-psu.edu (Rosemary A. Walsh)
Date: Sun, 11 Feb 1996 18:50:21 GMT
Subject: Re: MULTIPLE USERS ON THE SEM

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Microscopy ListServer {Microscopy-at-Sparc5.Microscopy.Com}

At 1:09 PM 2/9/96 -0500, Bonnie Davis - Kennametal Inc. wrote:
} I'd like some input, opinions etc. on the pitfalls of having multiple
} operators on an SEM. My situation is that I am part of a materials
} analysis group that provides analytical services to corporate
} technology. Our group consists of a number of engineers and technicians
} with expertise in a variety of analytical areas. Our company has
} recently hired a young PhD to do materials research in another department .
} He is pushing very hard to be allowed to use our SEM at night and on weekends.
} He has 'used
} an SEM before' and doesn't see a problem in using ours. It has always
} been our practice to encourage researchers to come into the lab while
} their samples are being analyzed, but not to allow individuals to use the
} instrument themselves. I believe that the analysis is done more effectively
} by individuals whose expertise is in that area. However, management
} appears to be leaning towards changing the rules for this individual, and
} having me train him on the instrument.
}
} We have a four year old JEOL 6400 instrument with a Link eXL analyzer,
} UTW detector, complete stage automation. We also have a computer system
} attached for collecting digital images, we do not use polaroid film.
} While this individual has used and SEM he has not used either a JEOL or
} LINK system before. I feel that training would be extensive. This is
} currently the only SEM we have. It
} is used approximately 12 hours per day by myself and my staff. Quite
} often EDS analysis is carried out using computer control and stage
} automation overnight. However, management feels that if someone is
} willing to utilize the instrument in those few hours a week when it is
} not being used, they need to take advantage of that to receive a maximum
} benefit from the asset.
}
} Several years ago we had two older SEMS and due to 'corporate
} downsizing', we had only one operator. At them time I trained
} approximately 2 dozen engineers who desired to use the 'extra' SEM on
} their own. We disposed of the instrument about a year ago when it had
} not been touched for almost a year. The time I spent in training
} all of those individuals was probably greater than the time they as
} a group spent using the instrument. Hindsight being perfect I wish we had
} kept the old instrument!
}
} Anyway, I would appreciate any input both pro and con from the list
} regarding opening up an instrument to multiple infrequent users.

Bonnie,
Since we encourage use of the JEOL TEM and SEM by trained users, we
run into this situation often. Currently, we require training on our
instruments plus a minimum of 15 hours of daytime use prior to an approval
of use during off hours. The principal investigator (of the grad. student
or postdoc) is also asked to agree to cover costs should any malfunction
necessitate lengthy (costly) alignment procedures. I constructed a form
for this purpose and the signed agreement is kept with the log book at the
scope. This has given me some control over the situation and those users
who agree to our demands are usually competent users. If the SEM uses a
LaB6 or is a FE, I would be more cautious, i.e., demand more daytime use
and obtain written consent to replace the crystal/filament.
One last thing to consider---an estimate of costs incurred by your
unit during the required "daytime" use if your unit does not charge other
units for instrument use.
Regards,
Rosemary Walsh






From: Richard Beanland +44 1327 356363 :      richard.beanland-at-gecm.com
Date: Mon, 12 Feb 1996 09:01:00 +0000 (GMT)
Subject: TEM: ferroelectric materials

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Disclose-Recipients: prohibited

Hello all,
I am currently doing TEM of ferroelectric materials (PZT, PST etc.)
and seem to be seeing domains or domain walls in thin TEM samples but not in
thick ones. I know that TEM of ferroelectrics has been done for some years -
does anyone on the listserver know of any references which describe the
contrast mechanism which makes them visible in TEM?

Many thanks in advance,

RIchard Beanland,
GMMTL Caswell,
Towcester,
Northants NN12 8EQ,
UK
Tel. +44 1327 356363
Fax. +44 1327 356775
Email richard.beanland-at-gecm.com





From: j_j_hooper-at-ecc-jkh.ccmail.compuserve.com
Date: 12 Feb 96 04:42:11 EST
Subject: Microscopy listserver

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Subscribe Microscopy j_j_hooper-at-ecc-jkh.ccmail.compuserve.com





From: Henrik Kaker, SZ - Metal Ravne :      Henrik.Kaker-at-guest.arnes.si
Date: Mon, 12 Feb 1996 09:43:08 +0000 (GMT)
Subject: Database

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Dear All,

All modern EDS systems include in the quantitative procedures an option
"standardless analysis". When standardless analysis is performed, tipically
the analysis of stainless steel is demonstrated with measuring transition
elements Fe, Cr, Ni, Mn, Co and V. The K-lines of these elements span in
the range 5 - 7.5 keV where the spectrometer efficiency is constant and
close to unity. The product is well behaved, and a reasonably accurate
analysis can be obtained, especially if the procedure has been "optimized"
for steel analyses. For testing standardless programs we need suitable data
base of measured intensity data with detailed information about analysis
(accelerating voltage, geometry) and detector constants. Unfortunately no
systematic collection of such data to be available in the area standardless
analysis. This is a sad situation in comparison with , for example analysis
with standards were there is an large collection of data. For this reason we
are in our laboratory decided to collect the intensity data for testing
standardless programs. Your help in adding new data sets would be much
appreciated. The rules for the data in the collection are very simple:

a) only experimental data from certified samples are included.

b) all data must be collected under known experimental conditions:
acclerating voltage, geometry (tilt angle, take-off angle) and
detector constants (Be window or other thickness, Au layer
thickness, Si dead layer thickness and Si crystal thickness).

c) only pure intensity data (after background substraction and peak
deconvolution procedure) are included.

d) weight fraction of analyzed elements must be known.

Final database will be available at EMMPDL (Electron Microscopy and
Microanalysis Public Domain Library) on anonymous FTP server WWW.AMC.ANL.GOV (Argonne
National Laboratory, IL, USA). We have no any commercial interest with
this database.

Thank you.

Henrik Kaker
Metal d.o.o.
SEM/EDS Laboratory
Koroska c.14
62390 Ravne
Slovenia

Phone: + 386 602 21 131 int.5562
Fax: + 386 602 20 436
E-mail: kaker-at-ctklj.ctk.si
http://www2.arnes.si/guest/sgszmera1/index.html






From: Richard Beanland +44 1327 356363 :      richard.beanland-at-gecm.com
Date: Mon, 12 Feb 1996 12:40:43 +0000 (GMT)
Subject: TEM: Ferroelectric materials

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Disclose-Recipients: prohibited

Hello all,
I am currently doing TEM of ferroelectric materials (PZT, PST etc.)
and seem to be seeing domains or domain walls in thin TEM samples but not in
thick ones. I know that TEM of ferroelectrics has been done for some years -
does anyone on the listserver know of any references which describe the
contrast mechanism which makes them visible in TEM?

Many thanks in advance,

RIchard Beanland,
GMMTL Caswell,
Towcester,
Northants NN12 8EQ,
UK
Tel. +44 1327 356363
Fax. +44 1327 356775
Email richard.beanland-at-gecm.com





From: Joe D Geller :      geller-at-world.std.com
Date: Mon, 12 Feb 1996 08:40:01 +0001 (EST)
Subject: Re: USGS TEM Lab - Free

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IS the Gatan Ion mill still available?
Joe Geller
508 887-7000


On Thu, 11 Jan 1996, Gordon L. Nord Jr. wrote:

} Dear Microscopists,
}
} Because of the recent downsizing of the US Geological Survey the TEM lab
} must go. In addition, because the last remanents of the Bureau of Mines (may
} it rest in peace) are coming to the USGS in Reston the lab space is needed for
} other purposes by next week (gasp). All or part of the following are available
} for transfer to US academic institutions. The USGS has not mentioned any
} possibility of help with respect to shipping but I could be surprised.
}
} (1) JEOL 200B Transmission Electron Microscope in working condition (1974) with
} STEM attachment (1978).
}
} (2) Multiple stages including Be double-tilt stage by Gatan and heating stage
} by JEOL.
}
} (3) Technics Ion Mill, 1974 (works)
}
} (4) Gatan Duo Ion Mill with low voltage guns and cooling stage, 1983 (works)
}
} (5) Tracor Northern 2000 Multichannel analyser and detector 1978 (works)
}
} (6) LogEtronics enlarger 1974 (works)
}
} Contact me at the following address.
}
} Cheers,
} Gordon
}
}
} Gordon L. Nord Jr.
} 959 National Center
} U. S. Geological Survey
} Reston, VA 22092
}
} Office: 703-648-6745
} FAX: 703-648-6789
}
} gnord-at-mactem.er.usgs.gov
}
}




From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 12 Feb 1996 10:48:14 -0500 (EST)
Subject: Re: message with no text

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Dear All,
It seems that I inadvertantly posted a message to this news-
group with no text. Please ignore.
Yours,
Bill Tivol




From: Stephan Helfer :      S.Helfer-at-rbge.org.uk
Date: Mon, 12 Feb 1996 15:30:01 BST
Subject: histology techniques (for botany) etc.

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Dear Microscopists
Could anyone advise me on current developments in the embedding (wax
or otherwise) sectioning and staining of botanical specimens for
histological and anatomical examination. We have got as far as
Histoclear, but most other techniques seem to be more than fifty
years old [and working satisfactorily, I may add]. Some of the old
stains are fading though and we have to replace old slides, and this
might be an opportunity to apply any up to date technique.

Cheers

Stephan Helfer
Royal Botanic Garden
Edinburgh
Scotland UK




From: waheeschen-at-dow.com (Bill Heeschen 517-636-4005 Materials/ASL)
Date: Mon, 12 Feb 1996 12:07:38 -0500
Subject: Re: Multiple User Question

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Message-Id: {199602121708.AA28812-at-na2.dow.com}

I must echo Bob Craig's observations about "external" users. He could have
easily written that word-for-word as a fellow employee here. Our back-up
plan for extended use of high-demand instruments is to go to an informal
swing schedule. Fortunately we have not had to resort to that officially,
but most of our gang have a lot of "after hours" time logged. One method
we have utilized for increasing throughput on our "automated" instruments
is to dial in from home with Timbuktu and AppleTalk Remote Access to check
on progress. If something has gone amuck, we can either fix it remotely or
zip in to work, fix it and resume the experiment. It's not pleasant to
come in at midnight instead of going to bed, but our customer's anxiety
(hence ours!) is greatly diminished the next day.

Bill
==========================
Bill Heeschen/Analyt. Sci.- Mat. Char. -----
1897-F Building / The Dow Chemical Co. --- ---
Midland, MI 48667 U.S.A. --- D O W ---
phone: (517)636-4005 fax: (517)636-5453 --- ---
Email: waheeschen-at-dow.com -----
========================== R




From: Igor Polyakov :      Igor_Polyakov-at-qmgate.arc.nasa.gov
Date: 12 Feb 1996 11:28:50 -0800
Subject: Antibody to TRH

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Subject: Time:11:04 AM
OFFICE MEMO Antibody to TRH Date:2/12/96

Hello Everybody,
I am looking for antibody to Thyrotropin-releasing-hormone for
immunocytochemistry which will work on rat brain. Does anyone know where I can
get it?
I. Polyakov
NASA-Ames Research Center
FAX: (415)604-0046
Email:Igor_Polyakov-at-qmgate.arc.nasa.gov





From: Dave King 857-1248 T37/257-3C Ext DEKING-at-VNET.IBM.COM
Date: 12 Feb 1996 09:29:02 EST
Subject: Multiple SEM Users

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To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}


This has been a problem for us at times. The real issues are; the
ability of people to work together, and the cost of maintenance,
and keeping the instrument in top condition. We find it possible
to have up to about 3 operators, as long as they can work
together well. They also need to be in the same department (i.e.
financed together). Pride in the condition of the instrument and
the quality of results is critical.

There can be other issues, like job security. If allowing someone
access you are putting someone else out of work...........

When the machine breaks, whoever had their hand on it, must
immediately get it fixed. This works best when there's a service
contract.If there's an assigned technician, this may not be a
problem for you.

My overall impression is that the number of operators must be
limited to a couple who can work together well, and not quibble
over who did what.

{
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Mon, 12 Feb 1996 17:03:43 -0600
Subject: ALL M: Detecting Fly Ash in River Sediment

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We have a researcher who wishes to determine if coal fly ash is present in
river water and river sediment. By LM I detect some "pearly" particulates
not present in control river sediment. Next step will be examination by
SEM.

My question: is there an established protocol for detecting and quantifying
fly ash in river water and sediment?

I will post any responses not sent to the server directly - unless
otherwise directed. Many thanks.

#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 12 Feb 1996 10:38:32 -0500 (EST)
Subject: Re: MULTIPLE USERS ON THE SEM

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Message-ID: {199602122331.SAA16597-at-IndyNet.indy.net}
To: Richard Beanland +44 1327 356363 {richard.beanland-at-gecm.com} ,
Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

[various snips]
} I'd like some input, opinions etc. on the pitfalls of having multiple
} operators on an SEM.
} Our company has
} recently hired a young PhD to do materials research in another department .
} He is pushing very hard to be allowed to use our SEM at night and on weekends.

He has to be trained very well to use the machine when no staff mem-
ber is around. It is much easier to have him use the SEM during the day, and
I would insist that he do so after training until you are absolutely satis-
fied that he is competant before allowing him to work without a net.

} He has 'used
} an SEM before' and doesn't see a problem in using ours. It has always
} been our practice to encourage researchers to come into the lab while
} their samples are being analyzed, but not to allow individuals to use the
} instrument themselves.

For our facility--a NIH biotech resource--we have several clas-
sifications for outside users, from novice, for whom the staff does every-
thing, to expert, who can use the instrument without supervision. We train
the user in many steps, the first being the basics for tilting and transla-
ting the specimen, focussing and taking a picture, then changing specimens,
later changing film, starting and shutting down the scope, etc. All this
takes several months for an in-house user, and longer for someone who visits
occasionally.

} This is currently the only SEM we have. It
} is used approximately 12 hours per day by myself and my staff. Quite
} often EDS analysis is carried out using computer control and stage
} automation overnight. However, management feels that if someone is
} willing to utilize the instrument in those few hours a week when it is
} not being used, they need to take advantage of that to receive a maximum
} benefit from the asset.

There is a large negative benefit if anything goes wrong; try to
impress management that a conservative approach is best.

} Anyway, I would appreciate any input both pro and con from the list
} regarding opening up an instrument to multiple infrequent users.

This will take a *lot* of your time. Good luck, you'll need it.
Yours,
Bill Tivol




From: Greg2NJ-at-aol.com
Date: Mon, 12 Feb 1996 19:54:57 -0500
Subject: Re: Image Processing Software

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Yes, there are various programs on the market. Depending on your budget, and
your need for continued support.
Probably the most ecconomic of the bunch is Image Pro Plus, soon to be
available using 32 bit, and windows 95.

The second PC based system, that I find very intuitive and has excellent
support is Kontron's KS 400 IA system. Kontron KS versions are distributed
and supported by Zeiss, Thornwood NY. The best person to contact is Uli
Kolhaaus, or Elise Shumpsy at Zeiss.

Gregory Argentieri
Sandoz Pharmaceuticals Corp
East Hanover, NJ
201-503-8617




From: Marilee.Sellers-at-nau.edu
Date: Mon, 12 Feb 1996 15:14:18 -0700 (MST)
Subject: Embedding Problems

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Hello everyone,
I have been embedding pinon pine root tips for mycorrhiza research.
We are close to solving most of our problems but still seem to have some
left. And I was wondering if some fresh ideas may help.
Problem: Portions (it seemed to be mainly the cortical tissue) of
the root tip will not completely infiltrate and polymerize. The mantel
looks okey and central vascular area seems to embed okey. The cortical
cells seem to be our main source of infiltration problems. Some of the
samples float (even after hours or days in a vacuum). At first, we used
Spurrs resin with graded Etoh. We have not used acetone or propylene oxide
(yet).

Figuring we were not getting all the water out of the tissue nor
getting our resin into the tissue we have tried the following procedures:
1. Cut our root tip sample even smaller.
2. We obtained some Z-6040 which has been mentioned several times on this
list for helping to infiltrate impervious biological specimens. We used it
at 1% as advised by Virginia Lindley's article. But we also continued its
use in the LR White resin as recommended by Stacie Kirsch at EMS.
3. We found we had to extend our Abs. ETOH times to over night and several
days of changes of LR White. We switched to LR White as recommended in
Lindley's article.

At this point, we had some success, but still had some root tips
that wanted to float (even under vacuum). I have not worked with root tips
or mycorrhiza before, but I may be doing more in the future. Do we need to
use acetone or propylene oxide to remove all the water?

Thank-you for any comments or helpful suggestions. If you should
like respond to me at my local address, I shall summarize for the listserver.

Good day,
Marilee

Marilee Sellers
Manager, Electron Microscope Facility
Northern Arizona University
Flagstaff, AZ 86011
E-mail marilee.sellers-at-nau.edu





From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 2/9/96 5:05 PM
Subject: Critical Point Drying

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I am searching for the most comprehensive articles about critical point
drying techniques. All suggestions are appreciated.

Any problems with going directly from ethanol into carbon dioxide or is
amyl acetate useful in between? My specimens look OK using just ethanol.

Also: I am experiencing a run of failing (most often between 400 and 800
psi) front window Dowty seals on my drying unit even if I do only 'dry'
(carbon dioxide only) test runs. Is anyone else having this problem? This
device has been operating for 17 years with few leaks. All sealing surfaces
are smooth and clean.

Thank you in advance.

Jim Romanow
Electron Microscopy Facility
Physiology and Neurobiogy Department
The University Of Connecticut
Storrs
bsgphy3-at-uconnvm.uconn.edu







From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Mon, 12 Feb 1996 15:54:21 -0600
Subject: Staining problem & static

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Message-Id: {199602122054.OAA28915-at-watson.bcm.tmc.edu}
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Thanks, Gib, for your message.

"....that some stray electrostatic charges floating around on these low
humidity winter days can precipitate beautiful hex lead crystals just
doesn't click for me...."

As I had said before, I also don't think that static charges would cause
inappropriate stain precipitation, but then as you can see from the response
on the listserver, a lot of people have experienced this problem and the
cause(es) have not been determined. At this point I am willing to try
anything and the suggestion didn't seem unreasonable.
***********************

"....I say chuck the whole damn works and switch to the Ted Pella "Grid
Stick" kit to do your uranium and lead staining...."

We have tried Pella's Grid Stick. However that procedure washes the sections
off of our grids. Our type of work necessitates the use of large sections
and 200 mesh "Super Grids" (grids with very thin grid bars). The use of
these grids results in much reduced area of contact between grid and
section, and the sections just do not adhere to the grids well. Now, if you
have any suggestions as to how we can get the sections to stick to the grids
better, I would appreciate them very much. Then perhaps we could institute a
more vigerous wash.
***********************

"....For lead, I use the Sato triple lead stain, not straight Reynold's lead
citrate...."

I do know of the Sato tripple lead stain, but would appreciate the
reference, if you have it handy. We have tried both the Reynold's recipe as
well as the recipe from Dr. Chandler Fulton. I don't have that reference.
However nothing could be simpler. To 10 ml of decarbonated, distilled water
you just add 0.02 gm lead citrate powder (EMS cat no. 17800), followed by
0.1 ml of 10N NaOH. This stain worked very well for me throughout my
graduate school experience.


Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 2/9/96 8:07 PM
Subject: MULTIPLE USERS ON THE SEM

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I'd like some input, opinions etc. on the pitfalls of having multiple
operators on an SEM. My situation is that I am part of a materials
analysis group that provides analytical services to corporate
technology. Our group consists of a number of engineers and technicians
with expertise in a variety of analytical areas. Our company has
recently hired a young PhD to do materials research in another department .
He is pushing very hard to be allowed to use our SEM at night and on weekends.
He has 'used
an SEM before' and doesn't see a problem in using ours. It has always
been our practice to encourage researchers to come into the lab while
their samples are being analyzed, but not to allow individuals to use the
instrument themselves. I believe that the analysis is done more effectively
by individuals whose expertise is in that area. However, management
appears to be leaning towards changing the rules for this individual, and
having me train him on the instrument.

We have a four year old JEOL 6400 instrument with a Link eXL analyzer,
UTW detector, complete stage automation. We also have a computer system
attached for collecting digital images, we do not use polaroid film.
While this individual has used and SEM he has not used either a JEOL or
LINK system before. I feel that training would be extensive. This is currently
the only SEM we have. It
is used approximately 12 hours per day by myself and my staff. Quite
often EDS analysis is carried out using computer control and stage
automation overnight. However, management feels that if someone is
willing to utilize the instrument in those few hours a week when it is
not being used, they need to take advantage of that to receive a maximum
benefit from the asset.

Several years ago we had two older SEMS and due to 'corporate
downsizing', we had only one operator. At them time I trained
approximately 2 dozen engineers who desired to use the 'extra' SEM on
their own. We disposed of the instrument about a year ago when it had
not been touched for almost a year. The time I spent in training
all of those individuals was probably greater than the time they as
a group spent using the instrument. Hindsight being perfect I wish we had
kept the old instrument!

Anyway, I would appreciate any input both pro and con from the list
regarding opening up an instrument to multiple infrequent users.





From: cgarri-at-mastnet.net (Craig Garrison)
Date: Mon, 12 Feb 1996 22:21:13 -0600
Subject: Multiple SEM Users

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A different perspective:

In a commercial organization, an instrument is a resource for all. The
question can not be is a person allowed to use a resource because they
are or are not a member of a particular work group. The question must
be how do we maximize productivity. Working within this framework, here
are two observations to balance:

It is typically counter-productive to have relatively inexperienced users
operate instruments which are either finicky or complex.

It is typically counter-productive to have expert users repeatedly perform
a relatively simple analysis.

Now for the reduction to practice. We have trained researchers from a
variety of groups to use our microscopes, OM, SEM, and TEM. This has
included training an inexperienced researcher on a FEG-SEM. We have never
had a mishap which cost us more than a few hours of instrument time. The
OM and SEM training has benefited productivity. However, TEM training has
been another story. Inexperienced researchers were repeatedly retrained,
resulting in an inefficient use of expert personnel.

Good luck to all,

Craig Garrison
The Dow Chemical Company
Bldg. B-1225
2301 N. Brazosport Blvd.
Freeport, TX 77541





From: petford-at-vax.ox.ac.uk
Date: Tue, 13 Feb 1996 09:23:25 +0000
Subject: TEM postdoc position

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I am looking for a postdoctoral researcher to fill the following post. Please
could any interested parties contact me directly.

Thankyou, Amanda Petford-Long.

*************************************************************
Advertisement for postdoctoral research post in the Department of
Materials, University of Oxford, UK funded by Hewlett-Packard.

A post is available, in the first instance for 1 year, to study layered films
for magnetoresistive read head applications. This will form part of an ongoing
collaboration with Hewlett-Packard Laboratories in Palo Alto, involving both
experimentalists and modellers within the Department of Materials.

The research will involve the characterisation of the microstructure and
magnetic domain structure, by electron optical techniques, of films grown at
HPL by sputter deposition. The films under investigation will be spin-valve
structures, and the interest lies in the role of microstructure on giant
magnetoresistance and magnetic properties. The microstructure of the films will
be analysed using high resolution electron microscopy, and we have already had
some considerable success in quantifying the nature of the interfaces between
the layers in the spin valve using imaging processing techniques on HREM
images, and would hope that this work could be continued. The magnetic domain
structure of the films will be studied using our extensive Lorentz transmission
electron microscopy facilities. These include facilities for in situ
magnetising and heating, and also for the collection of quantitat- ive
magnetisation maps. Some experience in electron microscopy (preferably HREM) is
therefore essential.

The post is fully funded by HPL on a rolling grant basis, so that further
funding after the 1 year period is extremely likely but cannot be guaranteed at
this time.

The post is available from 1st July 1996 and will be on the University salary
scale GBP14,317 - 21,511 p.a.

Interested applicants should submit their applications, including a curriculum
vitae and the names and addresses of two referees to the Administrator,
Department of Materials, Parks Road, Oxford OX1 3PH, UK. Please quote ref.
AKPL. The University is an Equal Opportunities employer.

For informal queries please contact Dr Amanda Petford-Long in the Department of
Materials. e-mail address: amanda.petford-long-at-materials.ox.ac.uk




From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Tue, 13 Feb 1996 07:34:05 -0500
Subject: Re: antibody to thyrotropin-releasing hormone

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Message-Id: {199602131232.HAA18482-at-lihti.org}
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Dear Dr. Polyakov:

Polyclonal antibodies to thyrotropin-releasing hormone are sold by:

AMERICAN RESEARCH PRODUCTS
489 Common Street
Belmont, MA 02178

Tel: (800) 832-2611, (617) 489-1120
Fax: (617) 489-5120

(Probably the best since it supplies them as IgG rather than serum).

A number of companies supply this antibody as serum, including

ACCURATE CHEMICAL AND SCIENTIFIC CO.
300 Shames Drive,
Westbury, NY 11590.

Tel: (800) 645-6264, (516) 333-2221, (619) 235-9400 (West Coast)
Fax: (516) 997-4948

(They also supply antibody to the free acid).

Others you might try:Arnel Products Company (Tel. 212-620-4622), Biogenesis
Ltd. (Tel. 603-887-4600, Email biogenesis-at-ltd.co.uk), Chemicon
International (Tel. 800-437-7500), Eurodiagnostics Lab (Sweden, fax
46-409231000), Cappel-Organon Technica (Tel. 800-523-7620) and UCB
Bioproducts (carried by Accurate Chemical and Scientific Corp, see above).

All these were raised in rabbit, so they should work on rat brain, although
I havn't tried any.


This is from the 1996 Lindscott's Directory of Immunological and Biological
reagents (Order from Lindscott's Directory, 4877 Grange Road, Santa Rosa,
CA 95404; phone (707) 544-9555, Fax (415) 389-6025), one of the best places
to start looking for this kind of information.

Richard D. Powell
*************************************************************
NANOPROBES, Incorporated
25 East Loop Road, Suite 124, Stony Brook, NY 11790-3350, USA
http://www.lihti.org/research/ecodev/incubten/nano/home.html






From: RMacKay :      RMACKAY-at-AC.DAL.CA
Date: Tue, 13 Feb 1996 10:06:14 +0000
Subject: Multiple Users

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Message-Id: {199602131409.KAA17548-at-Snoopy.UCIS.Dal.Ca}
Comments: Authenticated sender is {RMACKAY-at-ac.dal.ca}

RE: Multiple Users

Dear Bonnie,

We have operated a JEOL 733 electron microprobe ( presently with
a Link eXL system ) for the past 11 years, and prior to that a
Mark V. Our policy has always been to allow multiple users including
graduate and undergraduate students. In 25 years we have had only
one bad experience.

Best Regards,

Bob MacKay
Robert MacKay
Department of Earth Sciences
Dalhousie University
Halifax, Nova Scotia, Canada
B3H 3J5
Tel: 902 494-7087
e-mail rmackay-at-ac.dal.ca




From: Marilee.Sellers-at-nau.edu
Date: Tue, 13 Feb 1996 09:45:31 -0700 (MST)
Subject: Re: Embedding problems

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} Marilee, A microwave protocol is especially well suited for your work. I
} recommend checking the recent article by Giberson RT, Demaree RS, Jr.
Microwave
} fixation: understanding the variables to achieve rapid reproducible results.
} Microsc Res Tech 1995;32: 246-54.
}
} Gary R. Login, Beth Israel Hospital, Pathology
} 617-667-2034; glogin-at-bih.harvard.edu

Gary, This is one alternative I hadn't even considered. Thank-you. I do
not have a research microwave. But will see if ours would do. Marilee

Marilee Sellers
Manager, Electron Microscope Facility
Northern Arizona University
Flagstaff, AZ 86011
E-mail marilee.sellers-at-nau.edu





From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Tue, 13 Feb 1996 12:24:53 -0600
Subject: Ion Beam Damage

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I have a question which maybe someone can answer. Assuming
constant angle during ion-beam thinning, which of these two leads to
less nett damage (point defects etc) within a sample, assuming that
both remove the same amount of material:
a) A longer time at low energy
b) A shorter time at higher energy
?




From: ln-at-noesisvision.com (Luc Nocente)
Date: Tue, 13 Feb 1996 12:45:39 -0500
Subject: Re: Image PRocessing Software

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You can also try Visilog from Noesis Vision Inc. It provides the widest
selection of algorithms on the market along with an easy to use GUI and
macro language. We are soon coming out with a 32 bit version on both Unix
and Windows NT. You can contact me for more information.
Luc Nocente Tel: 514 345 1400
Noesis Vision Inc. Fax: 514 345 1575
e-mail: ln-at-noesisvision.com http://www.cam.org/~noesis
6800 Cote de Liesse, Suite 200
St-Laurent, PQ
H4T 2A7,Canada





From: Marilee.Sellers-at-nau.edu
Date: Tue, 13 Feb 1996 09:40:43 -0700 (MST)
Subject: Embedding Problems

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} Marilee,
}
} A year or so ago, we were working on a similar sounding project looking
} at mycorrhizal associations in pine roots (different pine). We were
} interested mainly in the composition of some inclusions but prepared material
} for general morphology too. We used a "standard glut/Fa" primary fix and an Os
} tet secondary followed by acetone dehydration and embedment in Spurr'Us or
} our own variant thereof (VCD and Quetol). We had no problem with infiltration
} or sectioning.
}
} Here's my twenty questions:
}
} 1. Do you use vacuum during fixation? I generally bounce the tissue
} during primary fixation by cycling the vacuum until the tissue stays
} submerged up to the boiling pressure of the fixative.

We have not used vacuum during fixation. Most of the time the tissue did
not float until after the Abs. Etoh. We shall include this.

} 2. Did you try to embed any of the floating material? I generally don't
} try to embed "floaters". My thinking is that if they have enough air in them
} to float after Os tet density increase, they've got too much air for good
} transfer of any thing into or out of the tissue.

We did embed some floaters. And they were poorly infiltrated.

} 3. When do you notice the problem in infiltration? i.e. during
} sectioning, or after observation. I've had occasional problems with LR white
} bonding to cell walls. Usually we can get enough to hold still for a picture
} if we carbon coat them after labeling and staining and before viewing. The
} stuff is supposed to be able to handle some water as an impurity, but I'm not
} sure I believe it.
} 4. What kind of knife do you use? I used glass since our root samples came
} from the real world and had entrained some sand as well as air. If yours don't
} have anything hard in them (or you'Uve got lots of money;-)) might use a
} diamond if youUre ot now. I cut a lot of hydroponically grown roots for class
} and generally get O.K. sections in regular resin with my diamond knife. Even
} they hydroponically grown tips have considerable exhaustible air in them.

We notice the problems immediately upon sectioning. These root tips are
from sandy soil, and the tips are cleaned as much as possible before
processing. As a precaution, we use glass knives to cut them.

} John Heckman
} TEM supervisor
} Center for Electron Optics
} Michigan State UniversityMarilee,
}
} heckman-at-pilot.msu.edu

Thank-you John. We will be incorporating your suggestions.
Marilee
Marilee Sellers
Manager, Electron Microscope Facility
Northern Arizona University
Flagstaff, AZ 86011
E-mail marilee.sellers-at-nau.edu





From: Bruce Cutler :      BCutler-at-eureka.chem.ukans.edu
Date: Tue, 13 Feb 1996 16:06:17 -0500 (CDT)
Subject: Multiple users

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View From A University Central Service Microscopy Lab

As the Director (and only employee) in a lab with a TEM, SEM,
confocal, imaging equipment, photographic darkroom, and the
usual ancillary equipment, and no service contracts, I could not
function if I had to do everything for users.
Ninety percent plus of my users operate the equipment
themselves. However, less than 5 users are 100% qualified to operate
the EM's when I am not present. I insert and remove specimens, and
for the TEM, process the negatives. Obviously, users undergo training,
and during training one can pick up cues as to the user's future
behavior. Our equipment is very user friendly, and that makes a big
difference.
Obviously in industry where down time is unacceptable, this approach
would not work.
Bruce Cutler, Microscopy Lab, Univ. Kansas, Lawrence







From: images1-at-biosci.mbp.missouri.edu (Michael Stanley)
Date: Tue, 13 Feb 1996 16:28:51 -0600
Subject: calcium on suspension cells

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Message-Id: {v01510101ad46bddb174e-at-[128.206.15.185]}
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Hello All,

We have a couple of users who want to look at calcium and/or pH changes on
suspension cells (culture cells). Does anyone know of specific tricks for
trying to stabilize single/suspension cells in a perfusion chamber. We
would like to follow a single/small group of cells throughout treatment
with different agents. We have tried both charged and coated coverslips
(silane and gelatin) without sucess. Any and all help would be greatly
appreciated.

TIA,

C. Michael Stanley, Ph.D.
Coordinator, Associate Director
Molecular Cytology Core Facility
Molecular Biology Program
2 Tucker Hall
University of Missouri-Columbia
Columbia, MO. 65211
(314) 882-4895
fax= 314-882-0123






From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 13 Feb 1996 16:56:00 -0500 (EST)
Subject: Re: Database Posting

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} The silicon 'dead layer' in a detector is a mathematical concept to
} describe a region in the detector of reduced sensitivity to the incoming
} x-rays (the Glasgow people coined a term for this which now eludes me).
} The 'dead layer' varies with the detector bias, and cannot be measured
} directly - it is possible only to deduce it from experiments fitting
} measured bremsstrahlung shapes to theoretical predictions.

Dear Tony,
The dead layer of a Si detector can, indeed, be measured experi-
mentally--at least in principle. Using an alpha source, measure the
energies when the alphas are incident on the detector both normal to
its face and at another angle for which the alphas still penetrate the
dead layer. From these measurements the thickness of the dead layer
(plus the gold) can be determined--at a number of bias voltages if
desired. Personally, I'd never subject my detector to this measure-
ment, but it is possible. We used to do this all the time for detec-
tors when I was in grad school.
Yours,
Bill Tivol




From: MelanieOwl-at-aol.com
Date: Tue, 13 Feb 1996 22:49:26 -0500
Subject: Re: MULTIPLE USERS ON THE SEM

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This is an interesting problem. I have had to train other users to use my
SEM, but have always found it to be a waste, since they didn't use it enough
to remember the training. I had to go over things each time they wanted to
use it.

However, if you are forced to let this person use the SEM, here are some
guidelines.
Mark the saturation point clearly, and make it a rule to not go past that
mark.
Have a log book handy for the part time user to record any 'unusual'
occurences in operation, or to write questions down.
Have an emergency number to call for help. (I know this is a pain for you,
but could save on instrument downtime and repair costs.)

That's all I could think of right now, but I hope it helps.

Regards,
Melanie Behrens
Senior Chemist
Texaco, Inc.
Beacon, NY
behrema -at- Texaco.com




From: Brett W. Cockman :      bcockman-at-uniwa.uwa.edu.au
Date: Wed, 14 Feb 1996 13:50:12 +0700 (WAST)
Subject: Fax No. needed

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Hello all,
I need to obtain the address and fax number of 'Triarch Slides' in Wisconsin, USA if
they are still in business.
Any help would be appreciated.
Regards,

Brett Cockman



----------------------------------------------------------------------------




From: CHAFFEYN :      NIGEL.CHAFFEY-at-bbsrc.ac.uk
Date: Wed, 14 Feb 1996 10:27:45 +0000
Subject: toluidine blue-quenching: a thank you

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Via: uk.ac.bbsrc; Wed, 14 Feb 1996 10:28:03 +0000
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Dear All,

Many thanks to all those who responded to my query re mode of action of
toluidine blue in quenching autofluorescence, particularly Bill Tivol and
Bridget Southwell who gave an answer that I can trot out if ever asked again!
In response to those who requested further details of the way I use
tol. blue: it is applied as a 0.01% (w/v) solution made up in
phosphate-buffered saline (pH nominally 7.3 - 7.6) to the sections (I routinely
use 6 um thick methacrylate-embedded Aesculus hippocastanum root sections).
Leave the stain to impart a 'light-blue' colour to the sections, ie, let them
'just stain', that takes about 90 sec with my tissue. Sections then mounted in
anti-fade mountant and viewed - stored if necessary in a fridge (at c. 4 C).
[Sections having been processed for visualisation of tubulin using FITC-linked
secondary antibody, and usually stained with DAPI prior to tol. blue-quenching]
"It works for me"...
I do not know if tol. blue itself fluoresces under some excitation
wavelengths, but it does reduce some of the background/native autofluorescence
present in my tissue (for Sally Stowe).
The reference I have used for this procedure is Baluska, F, Parker,
JS, Barlow, PW, 1992. Specific patterns of cortical and endoplasmic
microtubules associated with cell growth and tissue differentiation in roots of
maize (Zea mays L.). J. Cell Sci. 103, 191-200. Of course, if anybody can
provide an earlier (more definitive?) reference to its first use, I would be
grateful.
Hope the above is useful,

With best regards,

Nigel Chaffey [eMail: nigel.chaffey&bbsrc.ac.uk]




From: Stanley L Flegler :      flegler-at-pilot.msu.edu
Date: Wed, 14 Feb 1996 09:55:05 -0500 (EST)
Subject: LM needed

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I'm posting this request for a friend that needs to buy a light microscope. He
specifically is looking for a microscope with a large stage that will allow a
long working distance for using a micromanipulator. Does anyone have a used
microscope that they want to sell? Also, does anyone know the address or
telephone number of equipment brokers that might have light microscopes? If
so, please respond to Irv Widders, Michigan State University, E-mail
22457iew-at-msu.edu




From: Ciara Mullan :      mullanc-at-mcmail.cis.mcmaster.ca
Date: Wed, 14 Feb 1996 09:53:30 -0500 (EST)
Subject: Re: Ion Beam Damage

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I have found that (a), a longer time at low energy, is
better for my samples- III-V semiconductors.

Ciara

On Tue, 13 Feb 1996, L.D.Marks wrote:

} I have a question which maybe someone can answer. Assuming
} constant angle during ion-beam thinning, which of these two leads to
} less nett damage (point defects etc) within a sample, assuming that
} both remove the same amount of material:
} a) A longer time at low energy
} b) A shorter time at higher energy
} ?
}




From: george.braybrook-at-ualberta.ca (George Braybrook)
Date: Wed, 14 Feb 1996 08:21:24 -0700
Subject: Re: MULTIPLE USERS ON THE SEM

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Hi Bonnie,
One point that everyone seems to have missed on this subject of
multiple users is output quality. There is no substitute for the skill
gained with experience.
We tried running two SEM's in our lab, and found the overall
quality of the images produced by our "trained" multiple users was not as
good as the images produced by an experienced technician.

Cheers
:)
George
Department of Earth and Atmospheric Sciences
University of Alberta
Edmonton, Alberta T6G 2E3
Canada
ph: 403-492-5746
fax: 403-492-2030






From: cvierret-at-misn.com
Date: Wed, 14 Feb 1996 08:23:48 -0500 (EST)
Subject: Multi-User for SEM etc.

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There has been alot of comments on the matter of multi-user of and SEM. I
have been on both sides of that fence. I operated and SEM for years at the
Bureau of Mines until I took a research position. With my position I had the
need for SEM work. This I was able to do most by myself. I am now with a
company that does not have an SEM so I have to use the one at the university.
While I was the operator I was very choosey about who could use it. Only a
hand full of people asked to use it and when I started the training they
decided it was to much to remember and that I should do it because they did
not have the time to do it correctly. Now that I use someoneelses I am very
carful to follow all the instructions, I want to be able to come back. I also
felt very uncomfortable the first few times I was left alone with this
instrument, now I am familiar with it and use it much more. I guess what I am
trying to say is some people have no idea about an instrument and are hot shot
scientist, newly graduated PhD's and others. These are people to be aware of.
Multi-users are okay depending on the type of business you are in and the
personnal. I thought an opion from the other side should be expressesd.

Clarissa Vierrether
Analytical Development Chemist
The Doe Run Company
P.O. Box 500
Viburnum, MO 65566
1-573-244-8109




From: JOY-at-UTKVX.UTCC.UTK.EDU (DAVID JOY)
Date: Wed, 14 Feb 1996 10:08:39 -0400 (EDT)
Subject: Dead Layers in EDS detectors

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Message-Id: {199602141833.MAA06269-at-Sparc5.Microscopy.Com}

As Tony Garratt-Reed pointed out the 'dead layer' in an EDS detector is an
unfortunately chosen name. As first shown by Goulding (Nucl.Instrum. and Methods
142, 213, 1977) the 'dead layer' is simply the region over which drift anf
diffusion approximately cancel each other. The behavior of this region is
crucial when good resolution at low energy ( {2keV) is required. The MAS
Fiori Memorial volume "Xray Microanalysis in Electron Beam Instruments"
published by PLENUM contains two papers which discuss this in detail, and
also contain a bibliography of relevant references - one

Joy DC, "Modeling the Energy Dispersive X-ray detector", in "Xray
Spectrometry in Electron Beam Instruments", ed D B Williams, J I Goldstein
and D E Newbury, (Plenum Press:New York), 53-64, (1995)

which discusses a Monte Carlo simulation of this type of effect, and a second by
Jon McCarthy of NORAN which examines the practical implications of 'dead
layer' behavior. The dead layer can be estimated in several quite convenient
ways, including spectral measurements, matching to simulation ot, best of
all, by an electron beam measurement described in

Joy D C, "The EDS Detector - A Quantitative Model", Rev. Sci. Instrum. 56,
p1772-1779, 1985


As an interesting aside note that the dead layer in silicon at room
temperature is only 700 angstroms or so compared to 2800 A or more at
liquid nitrogen
temperatures

David Joy EM Facility, University of Tennessee





From: ychen-at-MACC.WISC.EDU
Date: Wed, 14 Feb 1996 12:57:39 -0600
Subject: Re: MULTIPLE USERS ON THE SEM

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} I'd like some input, opinions etc. on the pitfalls of having multiple
} operators on an SEM.

Bonnie,

The Integrated Microscopy Resource (IMR) is an NIH biomedical research
technology resource. We have a Hitachi S-900 FESEM with cryo-capability and
a YAG backscatter detector. As one of the few microscopy facilities open
for biologists, the novel instruments at the IMR are made available to
users world wide for biomedical research projects. Our user base consists
of senior investigators, post-docs, and graduate students.

As an NIH resource, the use of instruments is reserved for medical research
projects that require the specialized aspects of the available instruments.
Our SEM usage policy consists of three levels of approval: basic use,
backscatter detector use, and cryo-stage use. I provide user training and
supervise the use of the SEM for the first 20 hours. After this time,
approved users may be upgraded to unsupervised users who are permitted to
work independently after hours. Facility keys (either short or long term)
are available for unsupervised users. For this system to work it is
critical to give each user comprehensive instructions on use of the
equipment, to establish clear rules with regard to general use of the
facility, and most importantly to have an individual with the authority to
monitor and enforce these rules.

Our open use policy has been in place for 9 years with the SEM and 25 years
at our facility. Project approval and user training prior to equipment
usage has resulted in much success and very few problems. When problems
have arisen the solution has been for me to re-train the user and continue
to supervise them for longer time.

Hope this information answers your question.




Ya Chen

=========================================================================
\ / Assistant Researcher, Cryo/SEM Coordinator TEL : 608-263-8481
\ / __ Integrated Microscopy Resource (IMR) FAX : 608-265-4076
\/ / / University of Wisconsin-Madison
/ / / 1675 Observatory Drive #167 Email:YChen-at-macc.wisc.edu
/ /__/_ Madison, WI 53706 Email:chen-at-calshp.cals.wisc.edu

IMR WWW Home Page: http://www.bocklabs.wisc.edu/imr/imr.html
IMR Symposium on integrated microscopy '96: Sept. 20-22, 1996
=========================================================================






From: dan-at-isrv.com (Dan Focht)
Date: Wed, 14 Feb 1996 18:30:02 -0500
Subject: unsubscribe

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unsubscribe

Daniel Focht
Bioptechs, Inc.
3560 Beck Road
Butler, PA 16001
dan-at-bioptechs.com
Web page for Live-Cell Microscopy products
http://www.bioptechs.com






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Wed, 14 Feb 1996 17:48:30 -0600
Subject: EM: Calicivirus info

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Anyone have any good references re: Calicivirus? Especially interested in
classical virilogical studies (morphology, chemical characterization,
pathology, vectors, control measures) and current press releases on the
"epidemic" in Australia. Many thanks.


#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: DrJTS2-at-aol.com
Date: Wed, 14 Feb 1996 20:00:51 -0500
Subject: Subscribe

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subscribe drjts2-at-aol.com




From: Mark Wall :      Mark.Wall-at-quickmail.llnl.gov
Date: 14 Feb 1996 16:13:18 -0800
Subject: Mark Wall

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Message-ID: {n1387807448.72267-at-quickmail.llnl.gov}

Is it ok to post a call for papers/abstracts/exhibits on this microscopy
listing?

Mark A. Wall
Lawrence Livermore National Laboratory
L-350
7000 East Ave
Livermore, CA 94550
510-423-7162





From: Brett W. Cockman :      bcockman-at-uniwa.uwa.edu.au
Date: Thu, 15 Feb 1996 09:28:10 +0700 (WAST)
Subject: Re: Triarch contact details

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Hello all,
Thank you to all who contributed info on Triarch. If anybody knows of other
competitively priced prepared slide manufacturers I would appreciate their
contact details also.
Regards,

Brett Cockman




From: Greg :      greg-at-umic.umic.sunysb.edu
Date: Wed, 14 Feb 1996 17:18:10 EST5DST
Subject: Digitizing SEM images

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Hi Everyone,
I have a JEOL 5300 SEM to which a beam controlled image
acquistion system has been added. Images are acquired at
640x512 by frame averaging. It takes 1min 20sec. to
collect five frames. This is about the same time it takes
to do a Polaroid.
Here is the problem. At magnifications above 5000x the
images are fuzzy due to some kind of drift. At lower mags
the drift is not noticeable. Polaroids do not show the
drift. The drift rate is 100-500u per five minutes. I
have the SEM manufarturer working on this too. So far I
have not gotten acceptable results
Now for the questions. Has anyone had this problem on
other image acquisition systems? Am I trying to do
something that can't be done due to drift that all SEMs
have to some degree?
Thanks for your answers in advance.

Gregory Rudomen
Greg-at-umic.umic.sunysb.edu
University Microscopy Imaging Center
S.U.N.Y. at Stony Brook





From: caron-at-lisa.polymtl.ca (Mario Caron)
Date: Tue, 13 Feb 1996 23:25:47 -0500
Subject: SEM-Voltage contrast and EBIC

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I am looking for good references whose discuss about the experimental setup
(hardware, specimen preparation, experimental conditions, bias etc.) for
voltage contrast and EBIC experiments.
I am particularly involved in the fabrication of III-V high-speed devices
for signal capture (CCD and Sample&Hold).

Mario


*******************************************************
Mario Caron, M.Sc.A. ing.
Research assistant
Engineering Physics dept
Laboratory for the Integration of Sensors and Actuators
Ecole Polytechnique de Montreal
C.P. 6079, succ. `centre-ville`
Montreal, Quebec
H3C 3A7
http://lisa.polymtl.ca





From: James S MArtin :      James.S.Martin-at-williams.edu (by way of zaluzec-at-Microscopy.Com (Nestor J. Zaluzec))
Date: Wed, 14 Feb 1996 23:25:18 -0600
Subject: Thanks for SEM-EDS info

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Thank you to all who provided information on SEM-EDS services in and
around western Massachusetts.

James Martin







From: K. M. Anisur Rahman :      anis-at-execpc.com (by way of zaluzec-at-Microscopy.Com (Nestor J. Zaluzec))
Date: Wed, 14 Feb 1996 23:25:29 -0600
Subject: Does tin-oxide dissolve in Acetone?

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Hi,
Can anybody let me know if SnO2 and Sb2O5 would dissolve in
Acetone at about 100=B0C? Many thanks,

-Anis.







From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu (by way of zaluzec-at-Microscopy.Com (Nestor J. Zaluzec))
Date: Wed, 14 Feb 1996 23:25:39 -0600
Subject: Re: Re[2]: Staining Problems & static

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At 10:17 AM 2/8/96 EST, you wrote:

".....to form yellow, iridescent hexagonal prisms of lead iodide....."

I stained one grid with our lead stain alone, and another with our uranyl
acetate stain alone. The lead stained grid had the hexagonal deposits and
the other did not. So it's not the uranyl acetate. It's interesting that
lead iodide forms hexagonal crystals.
*******************

".....Perhaps these are your crystals - a bit of a long shot since you did
not mention iodine in our elemental analysis of the crystals...."

No, there is no iodine in either of our stains, or any of the sample
preparation steps; and I did not get an iodine peak with the microprobe.
*******************


I have borrowed an anti static gun like the Zerostatic Eliminator that you
suggest. We are in the process of trying it out. At least the grids don't
jump up and cling to the lid of the plastic petri dish any more.


* * Joiner Cartwright, Jr. * *








From: Bonnie Davis - Kennametal Inc. :      bld_kmt-at-prlc.org (by way of zaluzec-at-Microscopy.Com (Nestor J. Zaluzec))
Date: Wed, 14 Feb 1996 23:26:11 -0600
Subject: MULTIPLE USERS ON THE SEM

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I'd like some input, opinions etc. on the pitfalls of having multiple
operators on an SEM. My situation is that I am part of a materials
analysis group that provides analytical services to corporate
technology. Our group consists of a number of engineers and technicians
with expertise in a variety of analytical areas. Our company has
recently hired a young PhD to do materials research in another department .
He is pushing very hard to be allowed to use our SEM at night and on
weekends. He has 'used
an SEM before' and doesn't see a problem in using ours. It has always
been our practice to encourage researchers to come into the lab while
their samples are being analyzed, but not to allow individuals to use the
instrument themselves. I believe that the analysis is done more effectively
by individuals whose expertise is in that area. However, management
appears to be leaning towards changing the rules for this individual, and
having me train him on the instrument.

We have a four year old JEOL 6400 instrument with a Link eXL analyzer,
UTW detector, complete stage automation. We also have a computer system
attached for collecting digital images, we do not use polaroid film.
While this individual has used and SEM he has not used either a JEOL or
LINK system before. I feel that training would be extensive. This is
currently the only SEM we have. It
is used approximately 12 hours per day by myself and my staff. Quite
often EDS analysis is carried out using computer control and stage
automation overnight. However, management feels that if someone is
willing to utilize the instrument in those few hours a week when it is
not being used, they need to take advantage of that to receive a maximum
benefit from the asset.

Several years ago we had two older SEMS and due to 'corporate
downsizing', we had only one operator. At them time I trained
approximately 2 dozen engineers who desired to use the 'extra' SEM on
their own. We disposed of the instrument about a year ago when it had
not been touched for almost a year. The time I spent in training
all of those individuals was probably greater than the time they as
a group spent using the instrument. Hindsight being perfect I wish we had
kept the old instrument!

Anyway, I would appreciate any input both pro and con from the list
regarding opening up an instrument to multiple infrequent users.







From: gwe-at-biotech.ufl.edu (Greg Erdos) (by way of zaluzec-at-Microscopy.Com (Nestor J. Zaluzec))
Date: Wed, 14 Feb 1996 23:26:16 -0600
Subject: Re: PolyCutEase or SureCut Plastic additives

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} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

These things help a great deal when using a glass knife. We use lecithin in
the epoxy accelerator . ( use eual weight of lecithin and accelerator, then
double the volume for use) A glass knife will last a long time if you
have it in the resin. It was a great help to students just learning to
section, They could get usable stuff the first day at the microtome. It
can give a blotchy look to empty resin areas or those that have little
density, but most tissues are "busy " enough that you never notice it. See
Mollenhauer , 1986, J. EM Tech 3:217-222
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-








From: mager-at-unixg.ubc.ca (Mary Mager) (by way of zaluzec-at-Microscopy.Com (Nestor J. Zaluzec))
Date: Wed, 14 Feb 1996 23:26:34 -0600
Subject: Re: MULTIPLE USERS ON THE SEM

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} I'd like some input, opinions etc. on the pitfalls of having multiple
} operators on an SEM.

Dear Bonnie,
I run a Materials Engineering EM lab at the University of British
Columbia, with two SEMs and a TEM. Most of the researchers who use the
instruments are graduate students and Research Engineers. I have always
encouraged these people to do their own research on the SEMs, as much as
possible, since only they know exactly what they want and that way one me
can keep three instruments optimally used. After I show them how to use the
SEM, I watch and encourage them to ask me for further instruction for
picture taking, higher mag, etc. Some people, who have a lot of work to do,
may gain my permission to use the SEM after hours, but only after I feel
they have gained a lot of experience during working hours, when I am there
to supervise, and only after I have instructed them on how to properly shut
down and what to do in the event of problems. I also purchased the
instruments originally with ease-of-use in mind. They are fully automatic
and easy to get good results on.
I must feel that the person has a good understanding of the important
issues and knows how to properly handle the instrument. Mind you, I don't
have a field emission SEM. I have had damage, but only once in 15 years and
it could have happened in normal hours.. It also helps to scare them, be
"the ogre of the EM lab". Mind you, we are a teaching lab, so the learning
is as important as the doing.
I know many EM operators do not like the idea of letting any ham-fisted
graduate student or engineer loose on their instrument, but modern SEMs
really can be operated by any intelligent being.
So long as you satisfy yourself as to this person's knowledge,
experience and caring, I'd see no harm.
Hope this helps.

Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca









From: Glenn Poirier :      glennp-at-eps.mcgill.ca (by way of zaluzec-at-Microscopy.Com (Nestor J. Zaluzec))
Date: Wed, 14 Feb 1996 23:26:53 -0600
Subject: Re: MULTIPLE USERS ON THE SEM

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Hello Everyone,
I'd just like to put in my two cents on the subject of multiple users of
an SEM (or in my case an electron microprobe)

Our instrument is used at least 16 hours a day and since I'm only paid
for eight almost all our users are trained to use the machine
independantly. This allows them faster access to the machine and saves them
or their advisors money. The only people we don't encourage to become
independant users are those who will only use the machine once or twice.
Most users require 3-4 daytime shifts before i will let them use the
machine independantly (I give them my home phone number too).

In the six years I've been running the lab I can't recall one incident
where the machine has actually been damaged by inexperienced users. On
several occasions it has been temporarily put out of commission (computer
problems, etc) but usually I can get it back on line immediately the next
day. Although there is the potential for a user to damage the instrument
(i.e during a sample change) most of the rest of the instrument is fairly
fool proof. It is easy to screw up your analyses, but difficult to hurt
the machine.

I'm sure the machine would last longer and require less service if I was
the only one operating it, but we don't have that option. I also feel
that users are much better off acquiring their own data, they know what
they want and can change their strategy if they find something unexpected

Hope this is useful.

Glenn








From: jbpawley-at-facstaff.wisc.edu (Jim Pawley) (by way of zaluzec-at-Microscopy.Com (Nestor J. Zaluzec))
Date: Wed, 14 Feb 1996 23:27:00 -0600
Subject: Interested in purchasing cold stage for Philips microscope

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Hello all,

I have prototype LVSEM that employs the stage mechanism from a Philips EM
430 TEM. I would like to purchase a cold stage for this instrument if I
can find one (standard Philips rod). Anyone out there about to
"decommission" one?

Jim Pawley

****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Johnson Dr., Madison, WI, 53706 JBPAWLEY-at-FACSTAFF.WISC.EDU









From: rw9-at-psu.edu (Rosemary A. Walsh) (by way of zaluzec-at-Microscopy.Com (Nestor J. Zaluzec))
Date: Wed, 14 Feb 1996 23:27:06 -0600
Subject: Re: MULTIPLE USERS ON THE SEM

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At 1:09 PM 2/9/96 -0500, Bonnie Davis - Kennametal Inc. wrote:
} I'd like some input, opinions etc. on the pitfalls of having multiple
} operators on an SEM. My situation is that I am part of a materials
} analysis group that provides analytical services to corporate
} technology. Our group consists of a number of engineers and technicians
} with expertise in a variety of analytical areas. Our company has
} recently hired a young PhD to do materials research in another department .
} He is pushing very hard to be allowed to use our SEM at night and on weekends.
} He has 'used
} an SEM before' and doesn't see a problem in using ours. It has always
} been our practice to encourage researchers to come into the lab while
} their samples are being analyzed, but not to allow individuals to use the
} instrument themselves. I believe that the analysis is done more effectively
} by individuals whose expertise is in that area. However, management
} appears to be leaning towards changing the rules for this individual, and
} having me train him on the instrument.
}
} We have a four year old JEOL 6400 instrument with a Link eXL analyzer,
} UTW detector, complete stage automation. We also have a computer system
} attached for collecting digital images, we do not use polaroid film.
} While this individual has used and SEM he has not used either a JEOL or
} LINK system before. I feel that training would be extensive. This is
} currently the only SEM we have. It
} is used approximately 12 hours per day by myself and my staff. Quite
} often EDS analysis is carried out using computer control and stage
} automation overnight. However, management feels that if someone is
} willing to utilize the instrument in those few hours a week when it is
} not being used, they need to take advantage of that to receive a maximum
} benefit from the asset.
}
} Several years ago we had two older SEMS and due to 'corporate
} downsizing', we had only one operator. At them time I trained
} approximately 2 dozen engineers who desired to use the 'extra' SEM on
} their own. We disposed of the instrument about a year ago when it had
} not been touched for almost a year. The time I spent in training
} all of those individuals was probably greater than the time they as
} a group spent using the instrument. Hindsight being perfect I wish we had
} kept the old instrument!
}
} Anyway, I would appreciate any input both pro and con from the list
} regarding opening up an instrument to multiple infrequent users.

Bonnie,
Since we encourage use of the JEOL TEM and SEM by trained users, we
run into this situation often. Currently, we require training on our
instruments plus a minimum of 15 hours of daytime use prior to an approval
of use during off hours. The principal investigator (of the grad. student
or postdoc) is also asked to agree to cover costs should any malfunction
necessitate lengthy (costly) alignment procedures. I constructed a form
for this purpose and the signed agreement is kept with the log book at the
scope. This has given me some control over the situation and those users
who agree to our demands are usually competent users. If the SEM uses a
LaB6 or is a FE, I would be more cautious, i.e., demand more daytime use
and obtain written consent to replace the crystal/filament.
One last thing to consider---an estimate of costs incurred by your
unit during the required "daytime" use if your unit does not charge other
units for instrument use.
Regards,
Rosemary Walsh









From: loewe-at-uni-bonn.de (Andreas Loewe)
Date: Thu, 15 Feb 1996 02:58:22 -0600
Subject: Re: Ion Beam Damage

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I tried almost every possibility on our Baltec RES010.
I have found that (a), a longer time at low energy with one gun set to a
low angle and one gun to a rather high angle worked best on my ceramic
materials.

Andreas

______________________________________________________________
Andreas Loewe Tel: +49-228-550-355
University of Bonn Fax: +49-228-678-413
Insitute for Inorganic Chemistry email: loewe-at-uni-bonn.de
Inorganic Material Research
Roemerstr. 164
53117 Bonn
Germany http://www.elmi.uni-bonn.de/
______________________________________________________________






From: James R. Stets :      stetsjr-at-ttown.apci.com
Date: Thu, 15 Feb 1996 07:54:30 -0500 (EST)
Subject: Re: Drift in Digitized SEM Images

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I'm responding to Greg Rudomen's posting about fuzzy digital images on
his JEOL 5300.

Greg, can you give us some more details on your problem? It sounds like
you are using a slow scan to do your frame averaging since you say it
takes 1 min. 20 sec. to collect five frames.

Are you really seeing drift? Drift will generally be blurred in one
direction; if the images are just not sharp, that may be another problem.

When you say the Polaroids do not show the drift, are these Polaroids of
the analog or the digitized image? If the analog image is okay,
something is happening during digitization.

Another item to check is the power and magnetic field situation in your
lab. If the drift is more noticeable at low accelerating voltages, you
may have a field problem.

Feel free to contact me directly about this. I'm using a JEOL 6300F with
a Vision system, and it's no problem to obtain a clean 100K digital image.

Jim Stets
Air Products and Chemicals, Inc.
Allentown, PA
stetsjr-at-ttown.apci.com

Ye Olde Disclaimer: I speak for myself, not my employer.




From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Thu, 15 Feb 1996 10:42:26 -0500 (EST)
Subject: Re: EM: Calicivirus info

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Dear John,

I was once employed at Baylor College of Medicine in Houston, Texas in the
Division of Molecular Virology. One proffessor there did many studies on
Calicivirus. Her name is Mary K. Estes. I do not know her email address
but it is avaiable through Baylor's Web page address'. Hope you can obtain
the info you need through her.

Best of Luck,
Ed Calomeni
Medical College of Ohio
Dept. Pathology
Toledo, OH
emlab-at-opus.mco.edu




From: keller-at-boulder.nist.gov (Bob Keller)
Date: Thu, 15 Feb 1996 08:42:48 -0700
Subject: Digitizing SEM images

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Regarding the apparent drift problem in Greg Rudomen's images:

We have seen similar effects with a JEOL 6100 interfaced to a Voyager
EDS/image acquisition system. The effect is also noticeable when using the
image capture features of the SEM itself. Slow scans over a minute or two,
whether for a Polaroid or for digitizing, do not show the problem, as the
digitization occurs only once at each beam location and a small drift would
be unnoticeable in terms of seeing a distorted image; however, for
elemental mapping using single scans, with long dwell times resulting in
acquisitions taking hours, the drift/distortion is extremely evident.
Averaging, integrating or other algorithms which entail multiple scans show
the diffuseness very strongly. Most frustrating is that the problem is
sometimes worse during some sessions compared to others, with no obvious
differences in specimens or operating conditions.

This problem was suggested by the SEM manufacturer to be one of charging or
poor grounding in our specimens. Not likely, though, as we were observing
Si and GaAs crystals when the problem was most noticeable in images. The
problems were most notable with these because the magnifications were
considerably higher than other routine work done here. Decreasing the beam
energy to ca. 1-5 keV did not improve the problem, suggesting that charging
plays no role.

The problem is as yet unresolved, but I wanted to add our observations.
For the time being, I acquire images using slow scans only. Maybe it has
something to do with imaging on every third Tuesday of months ending with
the letter "y"...

My opinions only.

Bob Keller
NIST
Materials Reliability







From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Thu, 15 Feb 1996 11:30:40 -0600
Subject: Re: Drifting SEM images

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Message-Id: {199602151731.LAA05955-at-mailhub.iastate.edu}
X-Sender: wes-at-pop.ameslab.gov
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I got caught with some drift the other day on an E-SEM. The image was
drifting slowly in one direction. I half suspected charging, although the
drift was only in one direction and varying the pressure and cutting back
the beam did not help.

The problem was eventually tracked back to the modeling clay that I had used
to support the irregularly shaped sample. I was not aware that it outgassed
fast enough to change dimensions in the SEM. Now I know better.
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091
E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: randy nessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Thu, 15 Feb 1996 12:33:18 -0600 (CST)
Subject: Re: MULTIPLE USERS ON THE SEM

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} I'd like some input, opinions etc. on the pitfalls of having multiple
} operators on an SEM.

Hi all,
I snipped the above lines from Ya Chen's response to Bonnie. As
Nestor posted last week, there have been some difficulties with the
server, and it seems that I have missed some of the posts.
I hold a position at the University of Iowa's Central Microscopy
Research Facility. We are a multi-user resource for microscopy related
techniques. We have two SEMs that we train people to operate. ANYONE can
use our facility, as long as we train them, or/and they prove themselves
competent in the operation of the instrument. At that point, they are
issued keys, and can reserve the instrument to suit their schedule. We have
never had to ask for keys back from an individual, and any problems that
have occured were corrected with additional training.
We do have service contracts on our microscopes, and I can be
fairly handy with a tool box. Knock on wood, we have yet to have a
catastrophic event (one SEM is a field emitter). With the robust design
of modern electron microscopes, I don't anticipate any, either.
For more on the operating philosophy of our lab, as well as
instrumentation available, I suggest you visit our website at
http://www.uiowa.edu/~cemrf/.

Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu








From: randy nessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Thu, 15 Feb 1996 12:33:18 -0600 (CST)
Subject: Re: MULTIPLE USERS ON THE SEM

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} I'd like some input, opinions etc. on the pitfalls of having multiple
} operators on an SEM.

Hi all,
I snipped the above lines from Ya Chen's response to Bonnie. As
Nestor posted last week, there have been some difficulties with the
server, and it seems that I have missed some of the posts.
I hold a position at the University of Iowa's Central Microscopy
Research Facility. We are a multi-user resource for microscopy related
techniques. We have two SEMs that we train people to operate. ANYONE can
use our facility, as long as we train them, or/and they prove themselves
competent in the operation of the instrument. At that point, they are
issued keys, and can reserve the instrument to suit their schedule. We have
never had to ask for keys back from an individual, and any problems that
have occured were corrected with additional training.
We do have service contracts on our microscopes, and I can be
fairly handy with a tool box. Knock on wood, we have yet to have a
catastrophic event (one SEM is a field emitter). With the robust design
of modern electron microscopes, I don't anticipate any, either.
For more on the operating philosophy of our lab, as well as
instrumentation available, I suggest you visit our website at
http://www.uiowa.edu/~cemrf/.

Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu








From: Richard W. Fonda :      fonda-at-anvil.nrl.navy.mil
Date: Thu, 15 Feb 1996 12:15:49 -0600
Subject: Postdoctoral Position at NRL

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential

POSTDOCTORAL POSITION (US citizens only)

The Physical Metallurgy Branch of the Naval Research Laboratory is currently
looking for a postdoctoral researcher to study solid-solid phase
transformations. The ideal candidate would have a strong background in both
transmission electron microscopy and materials science (preferably with a
concentration in physical metallurgy). Current research interests center on the
study of solid-solid phase transformations in conventional and advanced metallic
alloys, ranging from fundamental studies of martensite and precipitate
morphologies in steels to microstructural analysis of low-carbon steel
weldments, advanced shape memory alloys, new beta-titanium alloys, and metallic
thin film and multilayer materials. Please contact either myself
(Fonda-at-anvil.nrl.navy.mil, 202-767-2622) or Dr. George Spanos
(Spanos-at-anvil.nrl.navy.mil, 202-767-5799) for further details about these and
other research programs.


EQUIPMENT
JEOL 200CX, Philips CM30, and Hitachi 9000 TEMs
Isothermal heat treatment facility for the study of rapid phase transformations
in an inert environment
Quenching and deformation dilatometer
Hitachi FEG-SEM
Auger electron spectroscope

PROGRAM
Descriptions of the postdoctoral programs at NRL may be obtained from Lesley
Renfro, Program coordinator, Code 1005.7, Naval Research Laboratory, Washington,
DC, 20375 (Renfro-at-utopia.nrl.navy.mil, 202-767-3865). Dr. Spanos is listed as
research advisor for the NRC program descriptions.

ELIGIBILITY
The postdoctoral programs at NRL are open only to US citizens.

_____________________________________________________________
Richard W. Fonda Naval Research Laboratory
(202) 767-2622 Code 6324
(202) 767-2623 fax Washington DC 20375
_____________________________________________________________





From: Patty Jansma :      plj-at-manduca.neurobio.arizona.edu
Date: Thu, 15 Feb 1996 15:45:14 -0700 (MST)
Subject: mounting media

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Could someone explain the difference between DEPEX and DPX mounting
media or are they used interchangeably?
If you have a fluorescently labelled sample cleared in methyl salicylate,
does anyone have a protocol for mounting in DPX? Do you have go back to
xylene before mounting in DPX or can you go directly to DPX from methyl
salicylate? How long does the DPX taken to harden on a 100 um sample?
Thanks.

Patty Jansma Tel:602-621-6671
plj-at-manduca.neurobio.arizona.edu
Arizona Research Labs Division of Neurobiology
University of Arizona






From: Denis Baskin :      baskindg-at-u.washington.edu
Date: Thu, 15 Feb 1996 15:29:31 -0800 (PST)
Subject: meeting announcement

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The following meeting may be of interest to some readers of this listerv.


WORKSHOP

PCR-IN SITU HYBRIDIZATION: =20
What you need to know to make it work

As part of the Histochemical Society Annual Meeting,=20
August 2-3, 1996,=20
Place: Hyatt Regency Hotel, Bethesda, MD

=09Topic: The use of PCR has revolutionized study of low-abundance=20
DNA and RNA molecules and recent advances have made it possible to=20
amplify DNA and cDNA targets in fixed cells and paraffin-embedded tissues=
=20
on slides. The use of these techniques is beginning to have a major=20
impact in both cell biology and diagnostic pathology. The workshop=20
will deal with: =20
=09=A5methodologies to label and detect nucleic acids using=20
non-radioactive primers=20
=09=A5advantages and disadvantages of PCR-ISH, pitfalls, controls,=20
optimal protocols, effects of fixatives, protease digestion, primers,=20
nucleotides, relevant enzymes and related issues. =20
=09=A5planned format includes lectures, question periods and=20
interactive demonstrations of in situ-PCR, PCR-in situ hybridization=20
and reverse transcriptase-in situ PCR. Thermocycling systems appropriate=
=20
for processing selected tissue specimens will be utilized and will=20
generally be available during the workshop. =20
=09=A5A syllabus will be provided for workshop participants.=20

=09Organizer: G. J. Nuovo, SUNY at Stony Brook


A related symposium will be on=20

ENHANCEMENT METHODS FOR IN SITU HYBRIDIZATION=20

=09Topic: Fluorescent in situ hybridization (FISH) techniques have=20
been utilized for the past decade and offer a widely used microscopical=
=20
tool providing high sensitivity and resolution, as well as the ability to=
=20
detect multiple targets. To increase the sensitivity of FISH, indirect=20
amplification by immunological methods has often been the method of=20
choice. This symposium will include the presentation of several novel=20
approaches using the enzymatic deposition of fluorescent reporter=20
molecules to amplify target localization. These methods may offer a new=20
approach to increase both the detection, resolution, and sensitivity of=20
the FISH method.=20
=09Organizer: S.L. Erlandsen
=09Speakers:=20
=09S. L. Erlandsen, University of Minnesota, Introduction.
=09A.K. Raap, Leiden University, Sensitive and high resolution FISH=20
=09B. Schmidt, Carnegie Mellon University, Signal amplification in=20
the detection of single copy DNA + RNA by enzyme catalyzed deposition=20
(CARD) of the novel fluorescent reporter substrate, Cy 3.29-tyramide.
=09V.L. Singer, Molecular Probes, Inc., The ELF alkaline=20
phosphatase substrate provides a bright, photostable, fluorescent signal=20
amplification method for FISH.=20

Another symposium will be on=20

APPLICATIONS OF MOLECULAR TECHNOLOGIES TO DIAGNOSTIC AND INVESTIGATIVE=20
PROBLEMS OF BREAST CANCER.=20

=09Topic: The symposium will include presentations on in situ=20
analysis of control of cell proliferation in breast cancer, molecular=20
markers of apoptosis and their correlation with hormone receptor=20
expression and other histologic prognostic markers and the structure of=20
breast tissue as it related to breast cancer development.=20
=09Organizer: A. M. Gown
=09Speakers:=20
=09H. Wolfe, Tufts University, Molecular markers of apoptosis and=20
their correlation with hormone receptor expression and other histologic=20
prognostic
markers.
=09R. Jensen, Vanderbilt University, BRCA1 gene expression in breast cancer
cells.
=09A.M. Gown, University of Washington, In situ analysis of control=20
of cell proliferation in breast cancer.
=09J.W. Gray, UC San Francisco, Applications of molecular techniques=20
to diagnostic and investigative problems of breast cancer.

POSTERS ON TOPICS RELATED TO THE WORKSHOP AND SYMPOSIA are welcome =20
(abstract deadline: March 15, 1996) and abstracts will be published as=20
Proceedings of the Histochemical Society Meeting in the Journal of=20
Histochemistry and Cytochemistry. .

CONTACT:=20
Inquiries about program matters and requests for registration materials=20
or abstract forms, and hotel accommodations should be sent to: The=20
Histochemical Society, 4 Barlows Landing Rd., Pocasset, MA 02559=20
(Telephone: 508-563-1155; FAX 508-563-1211 or e-mail: lmaser-at-mbl.edu). =20
or inquiries related to the scientific program to: Dr. W.L. Stahl=20
(e-mail: wlstahl-at-u.washington.edu).=20
=20






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Thu, 15 Feb 1996 22:45:50 -0800
Subject: Re: Digitizing SEM images

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Dear Greg,
Can you see the image drift at high mag. in TV-rate? Is this perhaps EM
field ripple? or mechanical vibration? The image acquisition system can
only acquire what the SEM puts out, so this drift will certainly wipe out
the benefits of frame averaging. If it takes so long to acquire a
frame-averaged image, why not just acquire one long frame? I have a
passive system and usually take a 10 sec., 1024X768 single frame, but the
image certainly shows up any drift of the SEM stage. This can always be
traced to the specimen not being secured well enough. Sticky tabs are often
guilty of this. If the SEM doesn't drift, the image is perfect to 50,000X.

One problem I have had in the past is bad vibration problems at 20 minutes
past the hour on class days. This is when classes change and two hundred
undergrad engineers tromp through the halls. People doing critical hi-res,
low kV work know to do it after hours, when the building is quieter and the
elevators aren't running.
Good luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: leeman-at-VOEDING.TNO.NL
Date: Fri, 16 Feb 1996 17:08:47 EST
Subject: LM - mounting medium

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Dear microscopists,

I am looking for a mounting medium which can be used for coverslipping
Giemsa stained cells containing latex microspheres. Xylene containing
mounting media cannot been used because of the solubility of the latex
microspheres. On the other hand, water soluble mounting media, like
gelatin/glycerin, destain the Giemsa. The staining is important to me to
clearly locate (for counting) the microspheres within the cell borders.
Counting without coverslip can be done but I prefere using a 40x/1.0
objective (oil).

Does anyone have experience with this problem?

Thanks in advance,

Winfried Leeman




From: Rick Markgraf :      rlmarkgraf-at-ucdavis.edu
Date: Fri, 16 Feb 1996 09:00:58 -0800
Subject: LM Pooling Teaching Microscopes

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I'm looking for other microscope pools, where teaching microscopes are
shared to maximize usage. I've worked for 20 years at Microscope Services
at the University of California, Davis. We have about 2100 optical
microscopes of high quality, and move 600-700 around every quarter from
classrooms where they aren't needed to those where they are. We also
provide preventive maintenance and repairs, short courses microscopes for
research, recharging for our services.
This arrangement works well at UC Davis, so it must be duplicated at
other schools. I just don't know where. If any of you know of schools with
similar arrangements, would you please post a contact for me? I would very
much like to hear from someone with similar tasks and problems.
Richard L. Markgraf
Microscope Services
University of California, Davis
Ph. (916)752-3477 Fax (916)752-6363
rlmarkgraf-at-ucdavis.edu





From: Larry Maser :      lmaser-at-mbl.edu
Date: Fri, 16 Feb 1996 10:51:05 -0500 (EST)
Subject: Microscopy & Microanalysis '96, Minneapolis, Minnesota

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Microscopy & Microanalysis '96

Joint Annual Meeting of the Microscopy Society of America,
Microbeam Analysis Society and the Microscopical Society of
Canada/Societe de Microscopie du Canada, August 11-15, 1996,
Minneapolis, Minnesota.

Deadline for receipt of Extended Abstracts: March 15, 1996.

Approximately 7,000 Registration Bulletin / Call for Papers
packages have been mailed, most on February 2, to: the
memberships of the three Sponsoring Societies; the memberships of
four Local Affiliates of the Microscopy Society of America
(Northwestern Ohio Microscopy Society, Minnesota Microscopy
Society, Southern California Society for Electron Microscopy -
Technologists, and the Iowa Microscopy Society); individuals who
attended Microscopy & Microanalysis '95, Kansas City; and other
individuals who have requested one.

Please contact our office if you should have received the
Registration Bulletin / Call for Papers but haven't, or if you
would like to. We'll respond to your request immediately.
Please note that the deadline for receipt of Extended Abstracts
is March 15, 1996.

Hope to see you in Minneapolis,

Larry Maser

Microscopy & Microanalysis '96
4 Barlows Landing Rd., Suite 8
Pocasset MA 02559

voice: 508-563-1155
toll free: 800-538-3672
fax: 508-563-1211
email: BusinessOffice-at-MSA.Microscopy.Com




From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Fri, 16 Feb 1996 10:28:14 -0600
Subject: Re: MULTIPLE USERS ON THE SEM

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Message-Id: {199602161628.KAA13875-at-mailhub.iastate.edu}
X-Sender: wes-at-pop.ameslab.gov
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From another university lab:

We too allow users on the scope after training as many in academic settings
appear to do. I routinely query the potential users about the realistic
duration of their work as we have trained some at their insistence only to
find out they have no specific project in mind, or that their actual SEM
usage was not nearly what they first imagined. Of course, since they are
charged for operator assistance, I guess we will continue to train them if
they care to pay for it.

Now a question,
We have adopted the practice of leaving the filament current control on our
JEOL 840A at the proper saturation point (for our normal 15 kv operating
point) and just turning off the high voltage control for exchanging samples.
We have thus avoided problems from inexperienced operators over-saturating.
It appears to be working as our filament lifetimes have increased to the
values for when only experienced users operated the scope. My question to
the list is whether there might be any adverse consequences from this
practice as there do not appear to be any.

----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091
E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Phil Elizondo :      PELIZONDO-at-svc.com
Date: Fri, 16 Feb 1996 11:42:38 PST
Subject: SEM Analysis position: Engineer

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Immediate Opening for SEM Analysis Engineer at SVC:


Principal Duties & Responsibilities:

SEM analysis of display components; basic failure analysis.
Responsible for upkeep of JSM 820, with full JEOL service contract;
Supervision & training of other SEM users.



Special work circumstances:

It is preferred that candidate be able to work either an early (start
-at- 6AM) or late (finish -at- 8PM) shift.



Experience & Education Requirements:

B.S. in Physical Science or Engineering or equivalent.
Minimum 3 yrs. experience in Electron Microscopy.


Company Background:

In just 4 years Silicon Video Corp. has become on of the hottest
start ups in Silicon Valley. We are developing a new class of flat
panel displays - Thin CRT. Millions in funding from the government
have been obtianed. Large corporate partners in industry and
academia connections have been secured. We're searching for highly
qualified candidates to join our team.

Please send resume response to both,

pelizondo-at-svc.com
dcaraway-at-svc.com







From: sfzane-at-unccvm.uncc.edu (Sandra F. Zane)
Date: Fri, 16 Feb 1996 14:28:27 -0500
Subject: SEM/stainless steel ball

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Hello All,
A graduate student here has been given the task of looking at the
surface of a highly polished stainless steel ball which is used in hip
replacements. The ball is about 2.5-3 cm in diameter and as you might
imagine, is extremely reflective. Since all I have ever examined in an SEM
are biological samples, I haven't been able to help her very much. This
morning we got what appeared to be a grid image on the surface and I am
wondering if this might not be a reflection of the interior surface of the
specimen chamber. I suggested sputter coating the surface to cut down on
reflection and mounting it on some double stick tape to keep it stable; but
she doesn't think the person who assigned her this task would want that.
Without regard to the wants of the person assigning the task, do any of you
in the materials science have any suggestions which might be helpful to this
young lady. She and I would both be very grateful.
TIA, Sandra Zane
Sandra F. Zane, EM Tech. sfzane-at-unccvm.uncc.edu
Dept of Biol., UNC Charlotte (704) 547-4051
9201 University City Blvd. Fax (704) 547-3128
Charlotte, NC 28223-0001








From: ychen-at-MACC.WISC.EDU
Date: Fri, 16 Feb 1996 10:57:17 -0700
Subject: RE:Digitizing SEM images

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} Hi Everyone,
} I have a JEOL 5300 SEM to which a beam controlled image
} acquisition system has been added. Images are acquired at
} 640x512 by frame averaging. It takes 1min 20sec. to
} collect five frames. This is about the same time it takes
} to do a Polaroid.
} Here is the problem. At magnifications above 5000x the
} images are fuzzy due to some kind of drift. At lower mags
} the drift is not noticeable. Polaroids do not show the
} drift. The drift rate is 100-500u per five minutes. I
} have the SEM manufarturer working on this too. So far I
} have not gotten acceptable results
} Now for the questions. Has anyone had this problem on
} other image acquisition systems? Am I trying to do
} something that can't be done due to drift that all SEMs
} have to some degree?
} Thanks for your answers in advance.
}
} Gregory Rudomen
} Greg-at-umic.umic.sunysb.edu
} University Microscopy Imaging Center
} S.U.N.Y. at Stony Brook
}

Gregory,
There are 3 kinds of averaging techniques used in SEM digitization: frame
averaging, line averaging and pixel averaging. For most of "built-in"
acquisition system, the frame capture is used, so only support frame
averaging. The drawback for using frame averaging is to produce a fuzzy
image as long as drafting exist. Pixel averaging is mostly suitable to
overcome this situation.

Ya Chen


Ya Chen
Cryo/SEM project Coordinator TEL : 608-263-8481
Integrated Microscopy Resource (IMR) FAX : 608-265-4076
University of Wisconsin-Madison Email:YChen-at-macc.wisc.edu
IMR WWW Home Page: http://www.bocklabs.wisc.edu/imr/imr.html
IMR Symposium on integrated microscopy '96: Sept. 20-22, 1996






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Fri, 16 Feb 1996 20:19:25 -0800
Subject: Re: SEM/stainless steel ball

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Dear Sandra,
It is very difficult to look at the surface of any highly-polished metal,
since there is nothing to focus on. However, you cannot get a reflection
image in an SEM except by charge buildup, so if you did get a grid image
from your secondary detector, it implies the ball is not well grounded. If
you stick it down, use conductive sticky-tabs or make a connection useing
silver or carbon dag. One help to focusing is to draw on the top surface
with a felt-tip pen, so you can find the initial focus. My experience
though, is that a good reflextive surface gives you very little to see,
even at high mag.
Good luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Fri, 16 Feb 1996 20:19:57 -0800
Subject: Re: MULTIPLE USERS ON THE SEM

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} Now a question,
} We have adopted the practice of leaving the filament current control on our
} JEOL 840A at the proper saturation point (for our normal 15 kv operating
} point) and just turning off the high voltage control for exchanging samples.
} We have thus avoided problems from inexperienced operators over-saturating.
} It appears to be working as our filament lifetimes have increased to the
} values for when only experienced users operated the scope. My question to
} the list is whether there might be any adverse consequences from this
} practice as there do not appear to be any.
Dear Warren,
We always leave the filament current up to the saturation point (or just
under it for maximum filament life). In fact, to stop little fingers, I
removed the filament current knob from my console years ago. I can change
it with a screwdriver when I need to. I always turn the acc. voltage on and
off and I get at least a month out iof a filament at 20kv. I don't know if
I would do it on my 200kv TEM, but SEM is fine.
Best of luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sat, 17 Feb 1996 10:02:09 -0600
Subject: Low energy vs high energy ion milling

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......Multiple Lines Removed.......

} The maximum energy available to impart to an atom in the bulk is given by the
} expression,
}
} E = E0 * 4* (Mm)/(M+m)^2,
}
} where M is the bulk atom mass, m is the ion mass and E0 is the ion energy.
}
} It seems to me that if you have sufficient energy for removal of ions, that you
} will always exceed the minimum threshold energy for displacent of atoms.

.........
Not true!

The sputtering rate/cross-section at the SURFACE of a material
is not the same as the displacement rate/cross-section in the bulk. The
simple way to
think about this is that a surface you only have ~ 1/2 the number of bonds
that you have in the interior of a bulk. Thus the energy needed to remove a
surface atom is LESS than that needed to remove one from the bulk. There
is extensive literature on this unfortunatley, I don't have the references
readily at hand here at home.

Charlie Bradley and I did work on this at ANL (for case of electron sputtering)
back in the late 80's some of which was published in the (E)MSA proceedings.
There is also an ANL Technical Report which tabulates both sputtering and
displacement cross-sections for the entire periodic table. The computer
programs which do the calculations for this are in the Software Library
which is accessible by anonymous ftp at

ftp.msa.microscopy.com

they are in the path /pub/3-MMSLib/HVEM

look for the files on DMottCS, TotCS, Recoil. Like I said, these programs
were written
for the case of electron sputtering, however, they could be modified for
the ion-beam case, if one were sufficiently interested. At any rate, you will
not displace the atoms in the bulk, but only the surface and near suface atoms.

There is another program called TRIM which is used by the Ion-Beam Analysis
community
to calculate bulk displacements in solids by Ions. I'll dig up the
references and
post them next week when I get back into the lab.

Nestor
Your Friendly Neighborhood SysOp.









From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Sat, 17 Feb 1996 12:59:51 EST
Subject: MultiUsers of EM equipment

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-- [ From: Garber, Charles A. * EMC.Ver #2.10P ] --

In response to:

} I'd like some input, opinions etc. on the pitfalls of having multiple
} operators on an SEM.

During the years 1963-1967, at what was then Case Institute (now CWRU),
during the conduct of my MS and Ph. D. thesis work, I did with my own
two hands, all of my own TEM work which at times involved in excess of
thirty hours instrument time per week over extended periods of time.
As a lowly (yes, we were definitely made to feel lowly) graduate
student, to this day, I remember how I had to tip-toe around the
manager of the EM facility. All of the graduate students had their own
keys both to the EM lab and also the darkroom, but one snap of the
manager's fingers could have keys snatched away in an instant. And for
the entire four years of my career as a graduate student, I did have my
own keys and for the most part, that part of my thesis work requiring
EM was done between the hours of 6:00 pm to 12:00 midnight (often times
later), and without supervision. Indeed virtually any graduate student
who had need for EM work was issued keys and for the most part, their
work was done during these "off hours" as well.

Indeed, to have the experience of actually being responsible for the
health and well-being of the instrument, and to be held accountable for
it, I always considered an important part of my own graduate
experience and education.

And sitting there, night after night, peering at the green image on the
screen, and experiencing that electrifying feeling that comes only when
seeing something for the first time, something perhaps no one else has
previously seen, was a part of my own life I will never forget. The
challenge of proving first to myself and then to others that what I
thought I had seen was in fact "real" and was not an "artifact" has had
a lasting positive impression on my own life and my own career for that
matter. After all, how else can one learn to be their own worst (best?)
"devil's advocate" if they have not done the work themselves?

How else can one "sell others" on the idea that what they have indeed
seen is real and not some preparation artifact? How many times have I
listed to presentations by one of those arm chair EM people and when
asked a question, could not even tell whether a sample was gold coated
or not! Or with what a TEM sample might have been stained. Not too
long ago I witnessed a speaker not being able to answer whether a
micrograph was by SEM or TEM. Such responses hardly evoke high levels
of confidence in someone else's conclusions.

Yet I can also remember some students who did not have research
projects depending that much on EM, perhaps their use of EM being
confined to closing a few lose ends, and where the inclusion of some EM
work was just a small part of their overall project. Indeed, either
because of less interest in doing it with their own two hands, or
perhaps other reasons, these students had that laboratory manager, the
one who made us jump through hoops, do the work, during daylight hours
with the student peering over his shoulders. On the other hand, there
were those who did have minimal need for EM work, but underwent the
extensive training voluntarily and with enthusiasm as part of their
overall graduate training experience.

I appreciated that lack of bureaucracy that existed in those days, and
the climate was such that the highest levels of curiosity were
encouraged. There is no question that if I was not able to have done
my own work, in the way that I did it, yes, perhaps even late at night
when there were fewer building vibrations and toilets flushing, I
would be a much different person. After all, at 1:00 am, when something
does go wrong, to deal with it, on your own, responsibily, to make
judgemental decisions, with no one else to turn to, is in itself an
important part of the educational process.

And what about down time? Of course there was the normal down time and
probably there was more down time than might have other wise been the
case because there were multiple users. But it was never an excessive
amount of downtime. And these were during the times when instruments
were not engineered like they are today, and if anything, relative to
modern instruments, the amount of downtime experienced should have
inherently been significantly more than would be the case today. But
excessive downtime never happened. Indeed seeing the way some of these
judgemental decisions were handled in those days has been valuable in
the management of my own analytical laboratory services business today.

I have followed the discussion on this listserver. I am really quite
surprised with the bureaucracies that seem to have evolved to somehow
"protect" the equipment from mere ordinary "mortals". What happened to
the ability to use the same kind of good judgment that abounded in the
EM laboratories of thirty years ago? Of course it is always easier to
go by way of a script and never deviate. Go by the book. After all,
no decision making has to be done at all. But something very very
valuable and fundamental is lost when those with the interest are not
able to sit down and take their own data, at their own speed, and on
their own terms.

I can appreciate the concern about minimizing downtime more than most
people, in my case, such costs come right out of my own pocket. It is
a very real concern. However, be it in industry or academia, give a
lot of thought before you shut out that inquisitive researcher who just
might make that big discovery that moves us all forward another notch.
A well managed industrial laboratory should have made provision for
emergency scope access anyhow so even if there was a bit of additional
downtime, surely that can not be thought of as throwing a monkey wrench
into that company's ability to have an instrument available to work on
solving emergency plant problems. If you have an instrument that is
that sensitive to multiple users, perhaps the time has come to look at
insturments made by some other manufacturer.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 17 Feb 96 13:23:53 EST
Subject: Re: Br-Methanol Polishing for TEM

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Dear Dr. Peiro-

At South Bay Technology we manufacture a Single Vertical Jet Electropolisher
which can be used for either electrolytic or chemical polishing. It is possible
to use it with Br-Methanol solutions and it does offer automatic termination as
you requested. It is also offers an automatic rinse option and several other
features. If you would like information on this product or on any of our other
sample preparation products, please feel free to contact me off-line. If you
have an interest, I can also put you in touch with our distributor in Spain.

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
sbt-at-msa.microscopy.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: Paul.Fischione-at-internetmci.com
Date: Sat, 17 Feb 1996 18:18:22 -0500
Subject: Ion Beam Damage

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Message-ID: {199602171916.OAA06508-at-IndyNet.indy.net}
To: "Sandra F. Zane" {sfzane-at-unccvm.uncc.edu} ,
Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

-- [ From: Paul E. Fischione * EMC.Ver #2.3 ] --

To expand on the ion energy vs. damage discussion, we have found that by
reducing the ion energy, a decrease in ion damage is experienced at a given
angle. During our research effort in developing the Hollow Anode Discharge
(HAD) ion source, we concentrated on achieving a broad range of ion energy
operation. Currently, the HAD source will operate with a minimum voltage
(accelerating) of between 500 and 800 volts and produce ion energies on the
order of a few microamps/square cm. At the maximum energy (6 kV), ion
current is in excess of 400 microamps/sq.cm resulting in very high milling
rates which are preferable when milling at low angles.

As with any preparation procedure, the key is defining the proper
parameters. Having a versatile ion mill that can effectively mill at
low/high angles, low/high voltages, and with liquid nitrogen cooling
affords the required flexibility.

There are some good references in the MRS Specimen Preparation Proceedings.

Regards,

Paul Fischione
E.A. Fischione Instruments, Inc.
9003 Corporate Circle
Export, PA 15632
Phone (412)325-5444
FAX (412)325-5443
e-mail paul.fischione-at-internetmci.com




From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Sat, 17 Feb 1996 17:55:50 -0600
Subject: Re: MultiUsers of EM equipment

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I would like to express very strong agreement with the sentiments of
Chuck Gabor; certainly within the academic setting protecting microscopes
from students is counterproductive. One thing I will argue is a credit
to our current system here is that we allow undergraduates to have
hands on experience using SEM's and TEM's.
While it is true that there are dangers of breakages, so what! To my
knowledge almost all the "big names" in electron microscopy made stupid
mistakes at one time or another - one example that I know of involved
switching of the vacuum and water lines on a TEM.

Laurie Marks




From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Mon, 19 Feb 1996 11:07:34 +1100
Subject: Re: Email virus...beware

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Message-ID: {MAPI.Id.0016.00683536202020203843394130303030-at-MAPI.to.RFC822}
Read-Receipt-To: Jean-Louis Beek {ah56-at-solo.pipex.co.za}
Priority: Normal
To: microscopy-at-Sparc5.Microscopy.Com
MIME-Version: 1.0

} There is a virus being sent about through the email system, it is
} usually sent with the subject (good news....). I received the below mail
} from my brother, who works with ICL. ICL sent the below message to all
} of it's employees.
}
} Please see the attached and take appropriate action.
}
}
} Issued by IT Services
}
}
} COMPUTER VIRUS
}
} There is a computer virus that is being sent across the Internet. If
} you receive an e-mail message with the subject line "Good Times," DO
} NOT read the message, DELETE it immediately. Please read the messages
} below.
}
} Some miscreant is sending e-mail under the title "Good Times"
} nationwide. If you get anything like this, DON'T DOWNLOAD THE FILE!
} It has a virus that rewrites your hard drive, obliterating anything
} on it.
}
} Please be careful and forward this mail to anyone you care about.
}
} *************************************************************
}
} WARNING!!!!!!! INTERNET VIRUS
}
} The FCC released a warning last Wednesday concerning a matter of major
} importance to any regular user of the Internet. Apparently a new
} computer virus has been engineered by a user of AMERICA ON LINE that
} is unparalleled in its destructive capability. Other more well-known
} viruses such as "Stoned," "Airwolf" and "Michaelangelo" pale in
} comparison to the prospects of this newest creation by a warped
} mentality. What makes this virus so terrifying, said the FCC, is the
} fact that no program needs to be exchanged for a new computer to be
} infected. It can be spread through the existing e-mail systems of the
} Internet. Once a computer is infected, one of several things can
} happen. If the computer contains a hard drive, that will most likely
} be destroyed. If the program is not stopped, the computer's processor
} will be placed in an nth-complexity infinite binary loop - which can
} severely damage the processor if left running that way too long.
} Unfortunately, most novice computer users will not realize what is
} happening until it is far too late. Luckily, there is one sure means
} of detecting what is now known as the "Good Times" virus. It always
} travels to new computers the same way in a text e-mail message with
} the subject line reading "Good Times." Avoiding infection is easy
} once the file has been received - do not read it! The act of loading
} the file into the mail server's ASCII buffer causes the "Good Times"
} mainline program to initialize and execute.
}
} The program is highly intelligent - it will send copies of itself to
} everyone whose e-mail address is contained in a receive-mail file or a
} sent-mail file, if it can find one. It will then proceed to trash the
} computer it is running on. The bottom line here is - if you receive a
} file with the subject line "Good Times," delete it immediately! Do
} not read it! Rest assured that the name was on the "From" line was
} surely struck by the virus. Warn your friends and local system users
} of this newest threat to the Internet! It could save them a lot of
} time and money.
}
} E N D O F N O T E
}
} --
} Tomaz Pereira Garcez (u01tp-at-abdn.ac.uk) +44 1224 632549

Diana van Driel
Dept Ophthalmology C09
Sydney University 2006
NSW, AUSTRALIA






From: ROBIN CROSS :      EURC-at-giraffe.ru.ac.za
Date: Mon, 19 Feb 1996 08:22:09 GMT+0200
Subject: digitizing SEM images

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From the various symptoms that have been described I think that
Rick Mott's last suggested source of this problem is most likely
to be correct. In conventional scanning the scan is usually
synchronized to the line frequency to minimize distortions
resulting from stray fields. When using an external scan
generator for acquiring digitized images some of the scan
speeds will be asynchronous with the line frequency. If there
are stray fields in the vicinity of the SEM these will then
cause some sort of image distortion on the screen, often showing
as a uniformly jagged appearance of what should be straight
edges. If one then averages a number of such frames it could
lead to what has been described as a fuzzy image.

In order to establish if this is the problem it will be
necessary to test for stray A/C fields around the SEM. These
usually arise from sources such as large power supply cables
running under the floor or in a nearby wall, but as Rick Mott
has suggested, could simply be a problem of bad or wrongly
routed earthing. If one doesn't have access to a proper magnetic
field testing coil an old EM lens coil connected to an
oscilloscope, although not calibrated, nevertheless does very
well for comparing one area with another for frequency and
magnitude of fields.

Good luck!


Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)




From: ROBIN CROSS :      EURC-at-giraffe.ru.ac.za
Date: Mon, 19 Feb 1996 12:27:47 GMT+0200
Subject: multiple users of EM's

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I have run an EM service unit for over 25 years during time which
the question of hands-on usage of the instruments has often been
discussed. This unit's size (4 EM's and a staff of four) is
probably common to many throughout the world and from what I
have seen of the present discussion on this issue our
problems/experiences/solutions are similarly shared by many
people elsewhere.

While there is no doubt that instruments will be better off if
only operated by trained full time EM Unit staff this policy is
seldom practical. Policies in force 25 years ago, when it usually
required the skills of regular operators to get the best out of
electron microscopes, no longer need to be so rigidly applied.
Today, not only is it easier for practically anyone to get good
results out of modern EM's with a minimum of training but also
it is much less likely that an inexperienced user will cause
any serious damage (with instruments such as our JEOL 1210,
filament saturation is pre-programmed and even if finger-happy
users create havoc with the beam the correct alignment can be
fully restored in one simple operation). We have found that to
work effectively the policy needs to be flexible and dependent
largely on two important factors:

- the nature and extent of the work
- the aptitude of the user for using this instrumentation.

In very general terms the policy successfully applied here is
that if a user's project is of the "once-off" variety (likely to
involve only one or two sessions) we will carry out or closely
supervise almost everything involving our equipment, but at the
same time encourage the user to share in the viewing,
interpretation and photography of images, etc. If the project
will require repeated use of the EM (more than, say, 3 or 4
sessions) then we find it worth our while to train that user to
operate the instrument more-or-less by themselves. The extent to
which this training extends depends on the anticipated amount of
usage and the perceived ability of that user to handle these
instruments. What generally happens is that the longer that
person uses the instrument the more procedures he/she will be
taught to perform. For a while this operation is confined to
just viewing and micrography with assistance being given in
other necessary operations such as specimen and film exchange.
Seldom, however, does user training extend to anything beyond
actual operation procedures (specimen exchange, viewing and
micrography). Although specimen preparation by properly trained
people is routinely allowed after hours in the EM Unit, use of
the EM's is very seldom permitted when no members of the EM Unit
staff are in the vicinity, mainly because of the validity of the
university's insurance cover (both for the user and the
instruments) in the event of an emergency under such
circumstances. Maintenance operations such as start- up,
alignment, filament exchange, etc, are not carried out by users.

Although it is sometimes seen to be discriminatory to have
one policy for one group of users and another for others, as
soon as the rationale behind the policy has been made clear
we have seldom had any further problems. As a result this
compromise policy works well and as long as our circumstances
remain as they are I see no reason to make any significant
changes.


Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)




From: Ostertag Tom :      ostertag_tom-at-mn15-gw.mavd.honeywell.com
Date: 19 Feb 1996 07:47:22 U
Subject: RE: Carl Zeiss Jena

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Message-Id: {199602191349.HAA01696-at-Sparc5.Microscopy.Com}

Jean-Louis Beek wrote:

} I have recently purchased an old Carl Zeiss Jena compound microscope , =

While on the subject, we have a Jenavert microscope that we inherited that is
in need of some parts to maintain tension in the stage adjustment. Our local
service provider has been waiting 6 months for these parts. Anyone have any
news about when we can expect to see our parts? How about any news about Carl
Zeiss Jena itself?

Tom Ostertag
ostertag_tom-at-mn15-gw.mavd.honeywell.com
Tom.Ostertag-at-mavdmh.honeywell.com
tostertag-at-tcm.mn.org
Minneapolis, MN










From: Walt Bobrowski (313) 996-7814 :      bobroww-at-aa.wl.com
Date: Mon, 19 Feb 1996 08:33:01 -0400 (EDT)
Subject: Re: Email virus..HOAX!

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Mr-Received: by mta PETVAX.MUAS; Relayed; Mon, 19 Feb 1996 08:33:01 -0400
Mr-Received: by mta PETVAX; Relayed; Mon, 19 Feb 1996 08:33:03 -0400
Mr-Received: by mta SRVR01; Relayed; Mon, 19 Feb 1996 08:40:44 -0400
Disclose-Recipients: prohibited
MSA Microscopy Mailing List {MICROSCOPY-at-Sparc5.Microscopy.Com}
Message-Id: {8601330819021996/A02465/PETVAX/11A29A210000*-at-MHS}
X-Envelope-To: dianavd-at-eye.usyd.edu.au, MICROSCOPY-at-MSA.Microscopy.com
Autoforwarded: false
Mime-Version: 1.0
Content-Type: TEXT/PLAIN; CHARSET=US-ASCII
Content-Transfer-Encoding: 7BIT
Importance: normal
Priority: normal
Sensitivity: Company-Confidential
Ua-Content-Id: 11A29A210000
X400-Mts-Identifier: [;8601330819021996/A02465/PETVAX]
Hop-Count: 2

The Good Times Virus threat is a complete fabrication that was started by some
members of America Online. Members should completely disregard posted messages
that perpetuate this myth that has assumed a life of it's own.

Walt Bobrowski






From: Vladimir Dusevich :      dusevich-at-astro.ocis.temple.edu
Date: Mon, 19 Feb 1996 10:40:06 -0500 (EST)
Subject: Re: SEM/stainless steel ball

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Optical reflectivness make no sense for SEM. Metallic samples are almost
ideal for SEM observation. I suppose, the only problem you have is focusing
on specimen which is about couple cm above stage surface. Try using full
focus range at minimum magnification.

Vladimir Dusevich
dusevich-at-astro.ocis.temple.edu


On Fri, 16 Feb 1996, Sandra F. Zane wrote:

} Hello All,
} A graduate student here has been given the task of looking at the
} surface of a highly polished stainless steel ball which is used in hip
} replacements. The ball is about 2.5-3 cm in diameter and as you might
} imagine, is extremely reflective. Since all I have ever examined in an SEM
} are biological samples, I haven't been able to help her very much. This
} morning we got what appeared to be a grid image on the surface and I am
} wondering if this might not be a reflection of the interior surface of the
} specimen chamber. I suggested sputter coating the surface to cut down on
} reflection and mounting it on some double stick tape to keep it stable; but
} she doesn't think the person who assigned her this task would want that.
} Without regard to the wants of the person assigning the task, do any of you
} in the materials science have any suggestions which might be helpful to this
} young lady. She and I would both be very grateful.
} TIA, Sandra Zane
} Sandra F. Zane, EM Tech. sfzane-at-unccvm.uncc.edu
} Dept of Biol., UNC Charlotte (704) 547-4051
} 9201 University City Blvd. Fax (704) 547-3128
} Charlotte, NC 28223-0001
}
}
}
}




From: John Getty :      jgetty-at-du.edu
Date: Mon, 19 Feb 1996 09:13:37 -0700 (MST)
Subject: Virus hoax

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Greetings,

An interesting article about the good times "virus" is located at:

http://www.du.edu/~buffer/jan96/goodtime.html

The bottom line is that the good times virus is a hoax. . . and
has received more time and attention from the Internet community
than it deserves.

John Getty
University of Denver
jgetty-at-du.edu






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Mon, 19 Feb 1996 11:54:07 GMT
Subject: Pathology/TEM

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A question for those doing thin section TEM for diagnostic pathology:

Is there a standard protocol for rapid processing of tissues where
overnight ( or sooner) results are required? I am talking straight morpholgy.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Nina S Allen :      nallen-at-unity.ncsu.edu
Date: Mon, 19 Feb 1996 11:39:04 -0500 (EST)
Subject: RE: Carl Zeiss Jena

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As most of you know, when Germany was reunited so was Zeiss aus Jena and
Zeiss aus Oberkochen. They are now one company. They are gradually
moving to East Germany. Nina Allen

On 19 Feb 1996, Ostertag Tom wrote:

} Jean-Louis Beek wrote:
}
} } I have recently purchased an old Carl Zeiss Jena compound microscope , =
}
} While on the subject, we have a Jenavert microscope that we inherited that is
} in need of some parts to maintain tension in the stage adjustment. Our local
} service provider has been waiting 6 months for these parts. Anyone have any
} news about when we can expect to see our parts? How about any news about Carl
} Zeiss Jena itself?
}
} Tom Ostertag
} ostertag_tom-at-mn15-gw.mavd.honeywell.com
} Tom.Ostertag-at-mavdmh.honeywell.com
} tostertag-at-tcm.mn.org
} Minneapolis, MN
}
}
}
}
}
}
}




From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Mon, 19 Feb 1996 09:17:58 -0600
Subject: Re: Email virus...beware

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Message-Id: {199602191518.JAA09603-at-mailhub.iastate.edu}
X-Sender: wes-at-pop.ameslab.gov
X-Mailer: Windows Eudora Pro Version 2.1.2
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Personally, my taste in humor runs in a different direction.

The "Good times virus" is a hoax. It has been around for several years now.
About once a month I hear about it again. And as often, those who know
better point out that it is a hoax. The most damage that comes from it is
the cluttering of the mail system with its false alarm and the
clarifications that it is a fake.

Viruses have to be executed, not just downloaded, to infect a machine.
Therefore, it is a good idea not to automatically open, execute, or run
attachments. Our "convenient" mail programs with their auto-this and that
features could do us a disservice.

The Macro viruses ARE making the rounds. These viruses hide out in
autoexecute macros in Word and Excel documents. Lest you think Microsoft
alone has a problem, any software that has such a macro capability is
potentially at risk. Still the document has to be opened for the macros to
execute.

I continue to see warnings about AOL-Gold and PKZip 3.0 viruses. I have not
seen them personally, but have been informed by reliable sources (CICA, et
al) that they are legitimate. PKZip's current version is 2.04; the 3.0
designation is bogus and carries the virus. AOL-Gold has a virus hidden in
the alleged install procedure. I thought AOL had pretty well stomped it out
and warned their subscribers.
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091
E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: jfmjfm-at-umich.edu (John F. Mansfield)
Date: Sun, 18 Feb 1996 22:14:51 -0400
Subject: Re: Email virus...ignore, it's a hoax.

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I would ignore this it is a resurfacing of a hoax from last year.


At 11:07 AM 2/19/96, Diana van Driel wrote:
} } There is a virus being sent about through the email system, it is
} } usually sent with the subject (good news....). I received the below mail
} } from my brother, who works with ICL. ICL sent the below message to all
} } of it's employees.
} }
} } Please see the attached and take appropriate action.
} }
} }
} } Issued by IT Services
} }
} }
} } COMPUTER VIRUS
} }
} } There is a computer virus that is being sent across the Internet. If
} } you receive an e-mail message with the subject line "Good Times," DO
} } NOT read the message, DELETE it immediately. Please read the messages
} } below.
} }
} } Some miscreant is sending e-mail under the title "Good Times"
} } nationwide. If you get anything like this, DON'T DOWNLOAD THE FILE!
} } It has a virus that rewrites your hard drive, obliterating anything
} } on it.
} }
} } Please be careful and forward this mail to anyone you care about.
} }
} } *************************************************************
} }
} } WARNING!!!!!!! INTERNET VIRUS
} }
} } The FCC released a warning last Wednesday concerning a matter of major
} } importance to any regular user of the Internet. Apparently a new
} } computer virus has been engineered by a user of AMERICA ON LINE that
} } is unparalleled in its destructive capability. Other more well-known
} } viruses such as "Stoned," "Airwolf" and "Michaelangelo" pale in
} } comparison to the prospects of this newest creation by a warped
} } mentality. What makes this virus so terrifying, said the FCC, is the
} } fact that no program needs to be exchanged for a new computer to be
} } infected. It can be spread through the existing e-mail systems of the
} } Internet. Once a computer is infected, one of several things can
} } happen. If the computer contains a hard drive, that will most likely
} } be destroyed. If the program is not stopped, the computer's processor
} } will be placed in an nth-complexity infinite binary loop - which can
} } severely damage the processor if left running that way too long.
} } Unfortunately, most novice computer users will not realize what is
} } happening until it is far too late. Luckily, there is one sure means
} } of detecting what is now known as the "Good Times" virus. It always
} } travels to new computers the same way in a text e-mail message with
} } the subject line reading "Good Times." Avoiding infection is easy
} } once the file has been received - do not read it! The act of loading
} } the file into the mail server's ASCII buffer causes the "Good Times"
} } mainline program to initialize and execute.
} }
} } The program is highly intelligent - it will send copies of itself to
} } everyone whose e-mail address is contained in a receive-mail file or a
} } sent-mail file, if it can find one. It will then proceed to trash the
} } computer it is running on. The bottom line here is - if you receive a
} } file with the subject line "Good Times," delete it immediately! Do
} } not read it! Rest assured that the name was on the "From" line was
} } surely struck by the virus. Warn your friends and local system users
} } of this newest threat to the Internet! It could save them a lot of
} } time and money.
} }
} } E N D O F N O T E
} }
} } --
} } Tomaz Pereira Garcez (u01tp-at-abdn.ac.uk) +44 1224 632549
}
} Diana van Driel
} Dept Ophthalmology C09
} Sydney University 2006
} NSW, AUSTRALIA

John Mansfield
North Campus Electron Microbeam Analysis Laboratory
413 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
jfmjfm-at-engin.umich.edu, John.F.Mansfield-at-umich.edu
or jfmjfm-at-umich.edu they all reach me!
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html






From: philf-at-NEWTON.UMSL.EDU (Philip Fraundorf)
Date: Mon, 19 Feb 1996 17:40:28 -0600
Subject: HREM of randomly-oriented 2D crystals

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Message-Id: {9602192340.AA05597-at-admiral.umsl.edu}
X-Sender: c4647-at-slvaxa.umsl.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
shsiriw-at-slvaxa.umsl.edu, s924939-at-UMSLVMA.UMSL.EDU,
s923023-at-UMSLVMA.UMSL.EDU, khbrewer-at-ucs.indiana.edu, FBaugh-at-aol.com,
swpgarv-at-slvaxa.umsl.edu, cs106-at-viper.wg.waii.com,
agray-at-ccsmtp.memc.com, dawkins-at-kepler.umsl.edu,
s922712-at-slvaxa.umsl.edu, s987041-at-slvaxa.umsl.edu, pnellesen-at-aol.com
X-Mailer: {PC Eudora Version 1.4}

Hi,

Can anyone suggest beam-stable specimens consisting of randomly-oriented
2D (i.e. molecule-thick) crystals for HREM (and other kinds of high
resolution) imaging? Simulations we've done (cf. Jun'95 Image of the Month
at {http://newton.umsl.edu/stei_lab/} ) suggest that they will give some
interesting contrast edge-on, even without sub-2A point resolution! I am
particularly interested in getting a TEM specimen of some large polycyclic
aromatic hydrocarbons (PAHS), like dicoronene, for comparison with images of
"2D graphite" in the cores of presolar interstellar graphite onions (cf.
Jan'96 Image of the Month, ibid.).

If anyone has any ideas how one might affect an abrupt transition from 2D
to 3D graphitic growth in the environs just outside the surface of a
carbon-rich red giant, I would love to hear them as well.

Cheers. /philf :)

//\/\/\/\---}
// Phil Fraundorf Physics&Astronomy/CME 314-5165044 philf-at-newton.umsl.edu
\\ B503 U.Missouri-SL St.Louis MO 63121 USA http://newton.umsl.edu/~philf
\\/\/\/\/\/\/\/---}





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Mon, 19 Feb 1996 18:09:57 -0600
Subject: TEM: Ultramicrotome Part

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X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
Message-Id: {v02130505ad4ec14ffa94-at-[131.230.97.68]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

We were given a Sorval MT-2 ultramicrotome which we have repaired and are
making ready for use. We need to locate a specimen chuck. Can anyone help?
Many thanks.

#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Greg2NJ-at-aol.com
Date: Mon, 19 Feb 1996 20:07:56 -0500
Subject: TEM related courses

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Looking for TEM related courses, seminars to attend for beginner to advanced
other than MAS and Scanning Inc.
Use and applications to Biological TEM
Biological Applications, specimen prep etc as related to TEM
Immunocytochemistry, autoradiography, etc. as related to TEM

Any and all leads would be appreciated.

Thanks
Greg2NJ-at-AOL.com
Gregory Argentieri
Sandoz Pharmaceuticals Corp
Electron Microscopy Lab
East Hanover, NJ 07936
201-503-8617





From: Donald Lovett :      lovett-at-trenton.edu
Date: Mon, 19 Feb 1996 21:54:49 -0500 (EST)
Subject: Re: Rapid processing of tissue samples for TEM

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REGARDING "Good Times"
To all;

This thing is the vampire of the net, it keeps showing up, sucking up
bandwidth and frightening people. I'd like to drive a virtual stake through
the heart of the individual or group that began this. The attachment to
this letter, I don't know if attachments show up on the server, has some
good information sources for virus information on this hoax and in general.

Where ever I go, there I
am.
Bob Lawrence




I know most of you know this, but you might like the phone #s


{ISWORLD-at-IRLEARN.BITNET}

Here is another view on the supposed e-mail virus circulating the
internet. Read through the forwarding notes to the CIAC newsletter.

--Jim

P.S. At this time of the year many of us accept student projects via
diskette or FTP. This latest virus scare provides us with a good reminder
that we should ALWAYS virus check anything we get off the net or from
our students.

=========================================================================

---------- Forwarded message ----------
} Reply-To: karyn-at-cheetah.llnl.gov
} Originator: ciac-notes-at-cheetah.llnl.gov
} Sender: ciac-notes-at-cheetah.llnl.gov
} Precedence: bulk
} From: Karyn Pichnarczyk {karyn-at-cheetah.llnl.gov}
} To: ciac-at-ncar.ucar.edu
} Subject: CIAC Notes 94-04
} X-Listprocessor-Version: 6.0b -- ListProcessor by Anastasios Kotsikonas
}
}
} U.S. DOE's Computer Incident Advisory Capability
} ___ __ __ _ ___ __ __ __ __ __
} / | /_\ / |\ | / \ | |_ /_
} \___ __|__ / \ \___ | \| \__/ | |__ __/
}
} Number 94-04 December 6,
1994
}
} ------------------- A - T - T - E - N - T - I - O - N -------------------
} | CIAC is available 24-hours a day via its two skypage numbers. To use
|
} | this service, dial 1-800-759-7243. The PIN numbers are: 8550070 (for
|
} | the CIAC duty person) and 8550074 (for the CIAC manager). Please keep
|
} | these numbers handy.
|
} -------------------------------------------------------------------------
}
} Welcome to the fourth issue of CIAC Notes! This is a special edition to
} clear up recent reports of a "good times" virus-hoax. Let us know if you
} have topics you would like addressed or have feedback on what is useful
and
} what is not. Please contact the editor, Allan L. Van Lehn, CIAC,
} 510-422-8193 or send E-mail to ciac-at-llnl.gov.
}
} $-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$
} $ Reference to any specific commercial product does not necessarily $
} $ constitute or imply its endorsement, recommendation or favoring by $
} $ CIAC, the University of California, or the United States Government.$
} $-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$-$
}
} THE "Good Times" VIRUS IS AN URBAN LEGEND
}
} In the early part of December, CIAC started to receive information
requests
} about a supposed "virus" which could be contracted via America OnLine,
simply
} by reading a message. The following is the message that CIAC received:
}
}
---------------------------------------------------------------------------
} | Here is some important information. Beware of a file called Goodtimes.
|
} |
|
} | Happy Chanukah everyone, and be careful out there. There is a virus on
|
} | America Online being sent by E-Mail. If you get anything called "Good
|
} | Times", DON'T read it or download it. It is a virus that will erase
your |
} | hard drive. Forward this to all your friends. It may help them a lot.
|
}
---------------------------------------------------------------------------
}
} THIS IS A HOAX. Upon investigation, CIAC has determined that this message
} originated from both a user of America Online and a student at a
university
} at approximately the same time, and it was meant to be a hoax.
}
} CIAC has also seen other variations of this hoax, the main one is that any
} electronic mail message with the subject line of "xxx-1" will infect your
} computer.
}
} This rumor has been spreading very widely. This spread is due mainly to
the
} fact that many people have seen a message with "Good Times" in the header.
} They delete the message without reading it, thus believing that they have
} saved themselves from being attacked. These first-hand reports give a
false
} sense of credibility to the alert message.
}
} There has been one confirmation of a person who received a message with
} "xxx-1" in the header, but an empty message body. Then, (in a panic,
because
} he had heard the alert), he checked his PC for viruses (the first time he
} checked his machine in months) and found a pre-existing virus on his
machine.
} He incorrectly came to the conclusion that the E-mail message gave him
the
} virus (this particular virus could NOT POSSIBLY have spread via an E-mail
} message). This person then spread his alert.
}
} As of this date, there are no known viruses which can infect merely
through
} reading a mail message. For a virus to spread some program must be
executed.
} Reading a mail message does not execute the mail message. Yes, Trojans
have
} been found as executable attachments to mail messages, the most notorious
} being the IBM VM Christmas Card Trojan of 1987, also the TERM MODULE Worm
} (reference CIAC Bulletin B-7) and the GAME2 MODULE Worm (CIAC Bulletin
B-12).
} But this is not the case for this particular "virus" alert.
}
} If you encounter this message being distributed on any mailing lists,
simply
} ignore it or send a follow-up message stating that this is a false rumor.
}
} Karyn Pichnarczyk
} CIAC Team
} ciac-at-llnl.gov
}
}
} ------------------------------
} Contacting CIAC
}
} If you require additional assistance or wish to report a vulnerability,
call
} CIAC at 510-422-8193, fax messages to 510-423-8002 or send E-mail to
} ciac-at-llnl.gov. For emergencies and off-hour assistance, call
1-800-SKY-PAGE
} (759-7243) and enter PIN number 8550070 (primary) or 8550074 (secondary).
} The CIAC Duty Officer, a rotating responsibility, carries the primary
} skypager. The Project Leader carries the secondary skypager. If you are
} unable to contact CIAC via phone, please use the skypage system.
}
} ------------------------------
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For years I have been using a protocol that allows me to section
materials the next day. It is modified from a booklet by Millonig:

Fix in buffered GA (3-4 hrs), rinse (45 min), osmicate (2 hrs), rinse and
dehydrate in acetone (3 hrs), infiltrate and embed in Spurr's (about 1 hr).
Instead of agitating each solution for several hours on a rotator to
infiltrate, Millonig recommends using a clinical centrifuge at 2,500 rpm
for 10-15 min for each solution. Polymerize overnight at 70oC. You can
section the next morning (though I prefer curing for an additional week).

This technique also eliminates the need to make multiple batches of resin
or to freeze the unused resin overnight.

Feel free to contact me directly for more details.


______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-trenton.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
Trenton State College, NJ 08650-4700 fax: (609) 771-2674






From: Gerd Schreiter :      G.Schreiter-at-mikrobiologie.uni-halle.de
Date: Tue, 20 Feb 1996 08:56:48
Subject: RE: Carl Zeiss Jena

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} As most of you know, when Germany was reunited so was Zeiss aus Jena and
} Zeiss aus Oberkochen. They are now one company. They are gradually
} moving to East Germany. Nina Allen
}
Right.

Their address:

Carl Zeiss Jena GmbH
Zeiss Gruppe
Tatzendpromenade 1 a
07745 Jena
Germany

Phone: +49 3641 64 0
Fax: +49 3641 642856

Sorry, but I can't find the email address.

Gerd

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
* *
* Gerd Schreiter Uni Halle *
* bgod7-at-mlubios8.mikrobiologie.uni-halle.de Inst. Mikrobiol. *
* G.Schreiter-at-mikrobiologie.uni-halle.de EM-Lab *
* Weinbergweg 16a *
* Tel. +49 345 5526369 Halle/S. 06099 *
* Germany *
* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *






From: Frik Koch :      FKoch-at-csir.co.za
Date: Tue, 20 Feb 1996 15:31:16 +0200
Subject: Please subscribe

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Disclaimer: The views expressed in this message are those of the
author, and not necessarily those of the CSIR and/or
it's employees.
Message-Id: {s129e944.051-at-csir.co.za}
X-Mailer: Novell GroupWise 4.1






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 20 Feb 1996 09:24:34 -0400
Subject: More on Alcatel 220 DiffPmp

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Message-ID: {n1387314190.84148-at-mse.engin.umich.edu}

Subject: Time: 9:10 AM
OFFICE MEMO More on Alcatel 220 DiffPmpOil Date: 2/20/96

In my last message I apparently gave some people the impression that Alcatel
220 diffusion pump oil is a polyphenyl ether fluid. This is not correct. It
is the synthetic hydrocarbon compound eicosyl naphthalene. As shown in the
graph of pump fluid characteristics on page 182, and as discussed on p 183,
of the reference previously cited (I've been asked to stop mentioning this
source directly, since some people think it smacks of commercialism), it has
vapour pressure and boiling point characteristics very similar to the
polyphenyl ether fluids Santovac-5 and Convalex-10, and is perhaps as good as
these fluids in regard to thermal and oxidative stability. I believe it is
also significangly less expensive than the PPE fluids. Factors to be
considered in changing from one pump fluid to another are also discussed on
p. 189 of said reference.
W. C. Bigelow (bigelow-at-umich.edu)





From: Scott Holt :      102467.2752-at-CompuServe.COM
Date: 20 Feb 96 10:57:20 EST
Subject: SEM of Stainless Steel Ball.

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Sandra,

The grid pattern that you said is appearing as a reflection of the SEM chamber's
interior sounds like a 'charge mirror'. This usually occurs if you have a
highly insulative surface which has been hit with a high energy beam to build up
a charge. Then, when the beam energy is reduced to 1 or 2 KeV, the beam is
repelled by the charge surface, scanning the screen of the secondary electron
detector instead. Since the reflected beam continues to raster normally, you
get an image of the detector screen instead of the sample. The problem is that
you are viewing a metallic surface which shouldn't be charging. Is there a
non-conductive coating on the ball?

One other possiblity...This is really unlikely, but I have done it before...
The opening of the chamber to the diffusion pump may have a screen covering it
(My JEOL 840A does). I have made the error of missing the sample altogether and
imaging this screen. Sounds ridiculous, but it's true. You may want to consider
this (like checking for your keys in the refrigerator).

Good Luck.

Scott D. Holt
BUEHLER, LTD.
41 Waukegan Rd.
Lake Bluff, IL 60044
Phone: (847)295-4546...New area code!





From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Tue, 20 Feb 1996 10:16:02 -0500 (EST)
Subject: Re: Pathology/TEM

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From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 20 Feb 96 12:30:51 EST
Subject: Re: TEM: Sorvall Ultramicrotome Part

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John-
I recommend you call Bill McGee at Microtome Sevice Co (315-451-1404). Bill
used to work for DuPont-Sorvall and is very experienced with parts and service
for the MT-2.
Steven Slap
102134,1660-at-compuserve.com





From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Tue, 20 Feb 1996 13:54:31 -0600
Subject: Electron Beam cleaning

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I have been persuaded (now to my regret) to give a talk about
electron beam cleaning. Having posted requests for information to a number
of logical sources (e.g. the surface science listserver) and only recieved
information requesting that I send THEM anything useful, let me try here -
at least we use electron beams! Has anyone heard of this and know any
useful references or links ?




From: levin-at-ecsuc.ctstateu.edu (Martin Levin)
Date: Tue, 20 Feb 1996 11:21:12 -0600
Subject: Calculating Area

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I would like to calculate the individual areas of muscle fibers in frozen
muscle sections. In the past I have used a planimeter and/or a simple grid
transparency. I would like to switch to a more "1990's" method, presumably
using a graphics tablet and the appropriate software. Basically, the
software would not only have to calculate individual fiber areas, but also
what percent of the entire muscle is made up of one fiber type or another.
Certainly a built in statistical package would be useful. If anyone could
recommend a set-up for use with a Macintosh, I would greatly appreciate it.
If there is another way besides a graphics tablet, I would certainly
consider that as well.

Thank you,

Martin A. Levin
Department of Biology
Eastern Connecticut State University
Willimantic, CT 06226
Phone: (860)465-4324 FAX: (860)465-5213






From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 20 Feb 1996 09:48:46 -1000 (HST)
Subject: Is this Hi-Res SEM?

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The boss is ready to send a manuscript off to a journal and has asked
for the definition of High Resolution SEM vs regular good res SEM. He's
looking at intramembrane particles of 10-20 nm on our Hitachi S-800
Field Emission SEM, which I lovingly always introduce as our Hi-res
SEM. Are there definitions in place for hi-res SEM and TEM? I see all
kinds of terms bandied about, but no numbers...

We also have a related query; Is anyone aware of any papers on non-cryo
SEM of intramembrane particles? Not freeze-fracture, not frozen,
hydrated, cryo SEM, and not AFM. We want to make sure to reference any
related work.

Thanks and aloha,
Tina

*****************************************
Tina (Weatherby) Carvalho *
Biological Electron Microscope Facility *
University of Hawaii *
(808) 956-6251 *
tina-at-ahi.pbrc.hawaii.edu *
http://www.pbrc.hawaii.edu/bemf/ *
*****************************************





From: dbd1-at-uclink4.berkeley.edu
Date: Tue, 20 Feb 1996 13:59:28 -0800
Subject: Chuck for Bozz

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I have a few extras. Flat or standard?


Doug Davis
Staff Research Associate
Electron Microscope Facility
University of California
Berkeley, CA 94720
(510) 642-2085
dbd1-at-uclink4.berkeley.edu







From: Elinor Solit :      cambrex-at-world.std.com
Date: Tue, 20 Feb 1996 18:22:22 +0001 (EST)
Subject: Re: Carl Zeiss Jena

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Mr. Beek,

Suggest you try Ernst Keller at Zeiss in Thornwood, New York. If he
can't help you directly, I'll bet he can put you in touch with those who can.

Their 800 # is 356-1090.

Good luck,

Ellie Solit
The Cambrex Group

On Sun, 18 Feb 1996, Jean-Louis Beek wrote:

} Hello everyone,
}
} Yet again I am resorting to the listserv for advise as it has never let me down.
}
} I have recently purchased an old Carl Zeiss Jena compound microscope , and would like
} to establish the value of it by a true connoisseur.
} A brief description:
} It is brass with a black base, a mirror as a light source, and a separate condenser for each
} objective. A circular stage with poloriser and analyzer.
} Complete in wooden box with extra eyepieces and objectives.
}
} Is anyone able to assist me over the net or do I have to resort to antique dealers.
} Looking forward to any help I can get.
}
} Regards,
} Jean-Louis Beek
} jlbeek-at-solo.pipex.co.za
}
}
}
}
}
}




From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Tue, 20 Feb 1996 11:17:14 -0500 (EST)
Subject: Re: Pathology/TEM

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Dear Greg,
When I need to rapid process tissues I use the following protocol:

1) Fix in 3% glut for 5 min. with a 30 sec. Microwave (MW) blast. (temp of solution
not to excede 50 C)
2) Rinse 2X with sodium cacodylate for 30 sec. each.
3) Post-fix in 1% OsO4 for 15 min. including a 30 MW.
4) Rinse in s-collidine 2X for 30 sec. each.
5) Tertiary fix in saturated UA for 15 min. with a 30 sec. MW.
6) Dehydrate in ethanol (30,50,70,90,95,32X-100%) for 30 sec. each using the
microwave for each step.
7) De-ethanate (like this word?) with 100% acetone 4X for 30 sec. each using
the microwave.
8) Infilitrate with a 1:1 mixture of acetone:Spurr's resin (rapid cure) for
1 hour with a MW blast every 10 min.
9) Place tissue in filled BEEM capsules and place in convection oven at 70 C.
10) Every 10 min. increase temp 2.5 C and at the end of 1.5 hrs remove blocks
and section. (do not excede 100 C)

Tissue should not be any bigger than 2mm, and assuming that you receive it in
formalin. If not received in Formalin, increase the glut fixation to
about 15 min.

In our lab any thing that is not done on the same day that we receive it, is
not considered STAT. We routinely try and get samples out (at least
on the scope) within 24 hrs of receiving them.

Hope this protocol works for you,

Best of luck
Ed Calomeni
Dept. Pathology
Medical College of Ohio
Toledo, OH 43699
emlab-at-opus.mco.edu






From: Sarka Lhotak :      lhotaks-at-fhs.csu.mcmaster.ca
Date: Tue, 20 Feb 1996 19:46:39 -0500 (EST)
Subject: TEM/Pathology

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On Mon, 19 Feb 1996, Greg Erdos wrote:

} A question for those doing thin section TEM for diagnostic pathology:
}
} Is there a standard protocol for rapid processing of tissues where
} overnight ( or sooner) results are required? I am talking straight morpholgy.

In our lab we use the following processing procedure for those occasional
rush clinical specimens:
It is assumed that the specimen is delivered to the lab in 2% GA. Cut
material into smaller pieces than for routine, approx. 1/2 mm3. Follow
the procedure bellow:

-sodium cacodylate (0.2M pH7.4) 2x3 min 4C
-osmium tetroxide (1%) in 0.1M NaCac 1x40 min 4C
-70% EtOH 2x5 min RT
-95% EtOH 2x5 min RT
-100% EtOH 3x10 min RT
-propylene oxide 3x5 min RT
-50:50 Spurr's:propylene oxide 1x20 min RT
-75:25 Spurr's:propylene oxide 1x20 min RT
-100% Spurr's 1x30 min RT

Embed in fresh 100% Spurr's and polymerize in an oven at 85 C for 1 - 1
1/2 hours.

Sarka Lhotak
EM Facility, Mc Master University
Hamilton, Ontario, Canada
lhotaks-at-fhs.mcmaster.ca





From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 20 Feb 96 13:24:57 EST
Subject: Ion Milling Damage

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While I do not have the definitive answer to the beam energy/angle incidence
debate, I do have several references that may be of help:

All of the following are by Dr. Arpad Barna of the Research Institute of
Technical Physics of the Hungarian Academy of Sciences. If you have an
interest, please contact me off-line and I can send you copies at no charge.

1) Topographic Kinetics and Practice of Low Angle Ion Beam Thinning

2) The Possibility of Surface Polishing by Ion Beam Thinning

3) Ion Beam Thinning on the Bases of Topographic Kinetics

4) Model Considerations of Ion Beam Thinning for Preparing TEM Samples

There are other papers dealing with specific sample sets such as diamond
coatings and other materials that may be of interest also. There is a lot of
good information in these papers but I don't recommend them for those who are
fainthearted as the math can be a bit intimidating at times!

South Bay Technology does sell the IV3 Ion Mill which is produced in Hungary and
is based on work done by Dr. Barna. Some proceeds, I believe, go to fund
research at the Research Insitute of Technical Physics. That being said, please
be aware that these are technical reports and NOT brochures on the ion mill!
The theory is applicable to any ion mill. I hope this information helps!

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
sbt-at-msa.microscopy.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: Sarka Lhotak :      lhotaks-at-fhs.csu.mcmaster.ca
Date: Tue, 20 Feb 1996 19:39:48 -0500 (EST)
Subject: TEM/Pathology

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On Mon, 19 Feb 1996, Greg Erdos wrote:

} A question for those doing thin section TEM for diagnostic pathology:
}
} Is there a standard protocol for rapid processing of tissues where
} overnight ( or sooner) results are required? I am talking straight morpholgy.

In our lab we use the following processing procedure for those occasional
rush clinical specimens:
It is assumed that the specimen is delivered to the lab in 2% GA. Cut
material into smaller pieces than for routine, approx. 1/2 mm3. Follow
the procedure bellow:

-sodium cacodylate (0.2M pH7.4) 2x3 min 4C
-osmium tetroxide (1%) in 0.1M NaCac 1x40 min 4C
-70% EtOH 2x5 min RT
-95% EtOH 2x5 min RT
-100% EtOH 3x10 min RT
-propylene oxide 3x5 min RT
-50:50 Spurr's:propylene oxide 1x20 min RT
-75:25 Spurr's:propylene oxide 1x20 min RT
-100% Spurr's 1x30 min RT

Embed in fresh 100% Spurr's and polymerize in an oven at 85 C for 1 - 1
1/2 hours.

Sarka Lhotak
EM Facility, Mc Master University
Hamilton, Ontario, Canada
lhotaks-at-fhs.mcmaster.ca




From: lag-at-pegasus.cc.ucf.edu (Lucille A. Giannuzzi) (by way of zaluzec-at-Microscopy.Com (Nestor J. Zaluzec))
Date: Tue, 20 Feb 1996 20:58:39 -0600
Subject: Hitachi HU-11E TEM parts available

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I have spare parts (sorry, an inventory of assorted stuff is not available)
from an Hitachi HU-11E TEM that is available for anyone who wants them.
Just pay for the shipping and it's yours.

Please respond to:


*************************************************************************
Lucille A. Giannuzzi, Ph.D.
Assistant Professor

Materials Science and Eng. Program
Dept. of Mechanical and Aerospace Eng. phone (407) 823-5770
University of Central Florida fax (407) 823-0208
4000 Central Florida Blvd. email lag-at-pegasus.cc.ucf.edu
Orlando, FL 32816-2450 USA
*************************************************************************










From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 20 Feb 1996 21:23:08 -0600
Subject: Re: Calculating Area

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Message-Id: {199602202113.QAA19783-at-hoh.mbl.edu}

} I would like to calculate the individual areas of muscle fibers in frozen
} muscle sections. In the past I have used a planimeter and/or a simple grid
} transparency. I would like to switch to a more "1990's" method,


The infinitely valuable (& Mac Native) image processing/analysis computer
program
called NIH Image written by Wayne Rasband, will readily do this work for
you. You will have to
first digitize an image of the muscle sections ( & a variety of options are
available to you here, flat bed scanners, TV cameras, slow scan CCD's) to
get the data
on to the Mac, but this should be easier than using the graphics tablet.
This will be your limiting step, but once done then you can digitize
areas & tabulate the results. By writing a simple macro you can automatically
compute % or export the results to a spreadsheet program.

Using various Operating System Emulators (Executor for the PC, MAE for Unix)
the program can also be run on Unix boxes & Windoze Machines. One commerical
manufacturer is developing a port of this program to the PC platform.


You can download a free copy of Image (current version number is 1.60)
from either the NIH ftp site
(zippy.nimh.nih.gov)
or the MSA ftp site
(ftp.msa.microscopy.com)
or the ANL AAEM WWW Site
(www.amc.anl.gov)
in all cases you should look for the "public" directory then filter
down the directory paths until you see the imaging/data processing area.

The documentation is extensive and very good.


Nestor Zaluzec
Your Friendly Neighborhood SysOp

P.S. I have no commerical interest in NIH ;-)






From: Marianne Ekwall :      Marianne.Ekwall-at-ah.slu.se
Date: Wed, 21 Feb 1996 09:42:30 +0000
Subject: Subscribe

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Message-Id: {199602210838.JAA27289-at-pinus.slu.se}
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From: John M. Libert :      jlibert-at-cpcug.org
Date: Wed, 21 Feb 1996 07:39:49 -0800
Subject: Re: Calculating Area

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Message-ID: {312B3CC5.3A33-at-cpcug.org}

Martin,
Any respectable image analysis software should be suitable. IPLab
Spectrum from Signal Analytics would be good for MacIntosh. Try NIH Image
first as it is free. In any case, the difficulty will be measuring the
individual fibers as segmenting the individuals from the mass via
thresholding may prove difficult without clearly defined boundaries. Some
image enhancement might be useful. Manual tracing might be necessary.

I can supply you with IPLab spectrum. I can supply you NIH Image with a
frame grabber board for your MacIntosh. SCION supplies Image with its
boards.

John
Martin Levin wrote:
}
} I would like to calculate the individual areas of muscle fibers in frozen
} muscle sections. In the past I have used a planimeter and/or a simple grid
} transparency. I would like to switch to a more "1990's" method, presumably
} using a graphics tablet and the appropriate software. Basically, the
} software would not only have to calculate individual fiber areas, but also
} what percent of the entire muscle is made up of one fiber type or another.
} Certainly a built in statistical package would be useful. If anyone could
} recommend a set-up for use with a Macintosh, I would greatly appreciate it.
} If there is another way besides a graphics tablet, I would certainly
} consider that as well.
}
} Thank you,
}
} Martin A. Levin
} Department of Biology
} Eastern Connecticut State University
} Willimantic, CT 06226
} Phone: (860)465-4324 FAX: (860)465-5213




From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Wed, 21 Feb 1996 10:12:35 -0500 (EST)
Subject: Re: TEM/Pathology

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As far as "RUSH" processing tissue for diagnostic EM here is the method
I've used for 15 years in a diagnostic EM lab. We routinely got good
results and 8x10 glossies by 4 that afternoon. Hope this helps.

"RUSH" Processing for EM
3
Tissue must be no larger than 1.5mm . Well fixed.(2.5% glut in
Dulbecco's PBS, pH7.3)

WASH...............PBS..................2X5 MIN.
1%OsO4 in PBS...........................20 MIN RTEMP.
WASH...............PBS..................2X5 MIN
*70% ETOH...............................3 MIN
95% ETOH................................3 MIN
100% ETOH...............................2X5 MIN
PROPYLENE OXIDE.........................2X5 MIN
1:1 P.O.:ARALDITE 502...................30 MIN (REMOVE Caps)
1:3 " " ...................20 MIN
PURE ARALDITE 502.......................10 MIN

EMBED

CURE IN 75C OVEN FOR 50 MIN. PLACE IN 95C OVEN FOR 50 MIN.
PLACE IN FREEZER AND LET COOL 10 MIN.

*ALCOHOLS MUST BE FRESH.

This method worked rather well when I was at GW University. If you have
any questions, call or e-mail me.

Peace,

Phil Rutledge, Director voice: (410) 455-3582
Center for Electron Microscopy e-mail: prutle1-at-gl.umbc.edu
UMBC
Dept. of Biology
5401 Wilkens Ave.
Catonsville, MD 21228





From: mdubey-at-FTMON.ARL.MIL (Dubey, Madan)
Date: Wed, 21 Feb 1996 14:13 -0500 (EST)
Subject: Isotro Wet etch Palladium

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Message-Id: {199602211734.MAA11806-at-thomas.ge.com}
X-Authentication-Warning: thomas.ge.com: daemon owned process doing -bs

Does any one know an isotropic wet etch for Palladium? I am trying to
make an etched pattern on Pd deposited on GaAs. The Pd is coated with
photoresist and should be wet etched for making the patterns.

Thanks;

M. Dubey
Research Physical Scientist
Physical Science Directorate
Advanced Devices Fabrication Division
AMSRL-PS-DB
Fort Monmouth, NJ 07703
Ph. 908-427-4040
Fx. 908-427-4306






From: Scott Williams :      scott_williams-at-pch.gc.ca
Date: 2/21/96 7:35 AM
Subject: Infrared microscope

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Does anyone know of a source for an infrared microscope? I have a professor
here that wants one.

Thanks

The following is an attached File item from cc:Mail. It contains
information that had to be encoded to ensure successful transmission
through various mail systems. To decode the file use the UUDECODE
program.
--------------------------------- Cut Here ---------------------------------
begin 644 RFC822.TXT



From: Scott Williams :      scott_williams-at-pch.gc.ca
Date: 2/21/96 7:35 AM
Subject: Infrared microscope

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end





From: Diana van Driel
Date: 2/18/96 9:22 PM
Subject: Re: Email virus...beware

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"microscopy-at-sparc5.microscopy.co" {microscopy-at-Sparc5.Microscopy.Com}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Reply to: RE} } Email virus...beware

For some information on "Good Times" see -
http://www.lbl.gov/ICSD/Security/
Mike O'Keefe
--------------------------------------

Diana van Driel
Dept Ophthalmology C09
Sydney University 2006
NSW, AUSTRALIA








From: leeman-at-VOEDING.TNO.NL
Date: Thu, 22 Feb 1996 08:24:55 EST
Subject: LM - BLOCKING ENDOGENOUS PEROXIDASE

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Dear microscopists,

A colleague of me is struggling with an immunostaining procedure.

Problem: Blocking the endogenous peroxidase in intestines from rats and mice in
cryo sections. Untill now we've tried Perhydrol in different concentrations,
vari‹ng between 0.3 and 2.4% with or without 0.1% sodium azide before, during
and after fixation. Upto now we did not succeed blocking the endogenous
peroxidase in cryo sections.

Who can help????


Winfried Leeman
TNO Food & Nutrition Research Institute
PO box 360
3700 AJ Zeist
the Netherlands
Voice: +31 30 694 44 97
Fax : +31 30 696 02 64






From: Microscopy-request
Date: Wednesday, February 21, 1996 2:13PM
Subject: Isotro Wet etch Palladium

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Does any one know an isotropic wet etch for Palladium? I am trying to
make an etched pattern on Pd deposited on GaAs. The Pd is coated with
photoresist and should be wet etched for making the patterns.

Thanks;

M. Dubey
Research Physical Scientist
Physical Science Directorate
Advanced Devices Fabrication Division
AMSRL-PS-DB
Fort Monmouth, NJ 07703
Ph. 908-427-4040
Fx. 908-427-4306






From: Neal Nicklaus :      nnicklaus-at-nova.sarnoff.com
Date: Thu, 22 Feb 1996 09:31:18 -0500
Subject: Re: Infrared microscope

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Depends upon your definition of "infrared". A near infrared (e.g. around 1
um) microscope may be purchased from Research Devices (NJ). Telephone
908-572-4800 and fax 908-572-4808.

Many standard microscope will also work in the near IR, just with poorer
quality. In fact, Research Devices used to take standard microscope
objectives, disassemble, re-space the components and reassemble.

You may adapt a standard microscope for near IR work by using a CCD camera
without color correction filter at the camera port or by using an image
converter. You need to check the optics of the specific microscope you have
to make sure there are no selective spectral coatings, etc.

Looking in a copy of the Photonics Buyers Guide (should be in your library)
yields a list of about a dozen vendors under "microscopes,infrared".
Depending upon your requirements you might also call them.

-----------------------------------
At 07:35 AM 2/21/96 CST, you wrote:
}
} Does anyone know of a source for an infrared microscope? I have a professor
} here that wants one.
}
} Thanks
}
}

Neal Nicklaus

SEQ Limited

Voice: 609-452-6033 Ext. 13
Fax: 609-452-5955
email nnicklaus-at-seq.sarnoff.com





From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 2/21/96 12:42 PM
Subject: EDX sum peak

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I'm looking at fused silica, quartz, trying to identify an inclusion

which I think is iron. I have a good K alpha iron peak, so all is well so

far, but also I have a large peak in an area where either Al or a sum peak

for the L alpha line of iron should be, but I have no peak at the area

where the L alpha should be.

My question is, can you have a sum peak, on EDX, for a particular

element, and not have a peak where the one should be that is causing the sum
peak?
I think the answer is yes, perhaps my counts are coming in too fast

and its doubling everything and showing a sum peak, but I thought I would ask.

Thanks,

Mark Darus

General Electric Co.

Darus-at-cle.dnet.ge.com





From: randy nessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Thu, 22 Feb 1996 10:29:35 -0600 (CST)
Subject: Dissolving polymerized acrylic?

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Hello Everyone,
I need to find out what solvent can be used to dissolve polymerized LR
White or Lowicryl. I am not sure which resin is the culprit. We have a
Reichert-Jung CS auto cryo-substitution unit, whose specimen substitution
chamber has become stuck in the substitution container (terminology taken from
the owners manual). Since it has been stuck for some time, and I wasn't
informed of the resin being used at the time the problem started, I can only
limit it to LR White or Lowicryl. A vendor of LR White suggested that 'acrylic
paint remover' might be the only hope. What common lab chemicals are in 'acrylic
paint remover'. Any other suggestions?
Thanks in advance,


Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu





From: roy-at-bayou.uh.edu (Roy Christoffersen)
Date: Thu, 22 Feb 1996 11:31:07 -0500
Subject: HRTEM with cryogenic sample holders

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What is the shared experience out there with HRTEM imaging using a
cryogenic (LN2) sample holder? I have used the ubiquitous Gatan-type of
cryogenic holder for reducing certain types of beam damage for CTEM and
AEM, but now I would like to do some lattice-fringe imaging using the
holder to limit beam-induced reduction of my oxide samples. How much will
my point-to-point resolution be degraded by vibration, drift or other
effects induced by the holder? On Gatan's Web page they seem to quote a
guaranteed resolution of around 0.5 nm with 0.34 "attainable". This is not
too encouraging for imaging oxide lattice spacings. I will try anyway, but
perhaps I'm bound to be disappointed....Can someone predict my future?

Roy Christoffersen
Texas Center for Superconductivity
3201 Cullen
Houston, TX 77204-5932
roy-at-bayou.uh.edu
(713) 743-8273
FAX: (713) 743-2787






From: philf-at-NEWTON.UMSL.EDU (Phil Fraundorf)
Date: Thu, 22 Feb 1996 12:29:09 -0600
Subject: Re: Infrared Microscope

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Robin Griffin wrote:
} Does anyone know of a source for an infrared microscope? I have a professor
} here that wants one.

Do you want a system which images things, counts things, takes spectra of
small regions, or all three? What sizes are the things you want to look at?
In or on what kind of substrate?

These are questions to answer before I even know if I could point you in the
right direction. For example, there are IR systems which can count solid
state defects only 20nm in size, even providing spatial coordiants for them
in three dimensions, but nonetheless form neither images (certainly not at
THAT resolution!) nor spectra.

Cheers. /philf :)

//\/\/\/\---}
// Phil Fraundorf Physics & Astronomy/CME 314+5165044 philf-at-newton.umsl.edu
\\ B503 U.Missouri-SL St.Louis MO 63121 USA http://newton.umsl.edu/~philf
\\/\/\/\/\/\/\/---}





From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 22 Feb 96 15:00:53 EST
Subject: dissolving polymerized L.R. White

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Message-id: {25535595-at-dancer.Dartmouth.EDU}

I get rid of errant L.R. White with 95% ethanol soaking.
Kate Connolly




From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Thu, 22 Feb 1996 13:58:43 -0500
Subject: Re: ISO 9000 Certification

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Contrary to the suggestion that this discussion be off-line, I feel this is
an area that needs greater awareness and implementation in microscopy
laboratories. There are two issues here: the quality system chosen, and
the method of implementation.

The ISO 9000 series is production and supply oriented, but does contains
standards applicable for the laboratory. ISO/IEC Guide 25 is more
laboratory oriented. In a short paper at a 1994 Paper Industry conference,
one of my colleagues compared these with each other and the ANSI-ASCQ Q2
standards for laboratory quality.

Regardless of the system chosen there are many things that need doing in a
microscopy laboratory to establish a quality program. They include
documentation of operating procedures, regular checks of microscope and
EDX performance (signal strength, resolution, magnification calibration),
logging of all instrument repairs and adjustments, analytical data review,
etc.. As a practical matter it has been difficult to achieve the
discipline and establish priority to execute performance tests on a regular
basis.

I would like to hear from others who have implemented quality programs as
to their experiences.

David Rothbard

--
Institute of Paper Science and Technology






From: RonMervis-at-aol.com
Date: Thu, 22 Feb 1996 17:24:35 -0500
Subject: Mounting media for coverslipping

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Hi Netters...
I have a question for you histology/microscopy mavens out there... we have
been embedding our Golgi-stained tissueblocks in low viscosity
nitrocellulose...the cut sections are cleared in alpha-terpineol and after
several thorough rinses in xylene we have coverslipped the tissue using
Permount (Fisher Scientific)...
Recently, we have also tried using Shandon mounting mediums [Shandon-Mount
and E-Z Mount] (which are methacrylate polymer-based)...we have noticed an
occasional cloudiness in the rim of nitrocellulose surrounding the
tissue....is there something that we can do to eliminate this??? (although
it's more noticable in the nitrocellulose rim, I concerned that the
cloudiness may extend into the tissue itself and might impair
observations...)
Thanks in advance....
Ron Mervis (RonMervis-at-aol.com)
NeuroMetrix Research
tel: 614-486-6080
fax: 614-486-6020




From: Rudolf Oldenbourg :      rudolfo-at-mbl.edu
Date: Thu, 22 Feb 1996 17:18:53 -0500
Subject: Postdoc Position at MBL

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Message-ID: {312CEBCD.2B75-at-mbl.edu}

At the Marine Biological Laboratory in Woods Hole, Massachusetts, is a
Postdoc position available for developing 3-D optical sectioning,
deconvolution and reconstruction techniques applicable to polarized
light microscopy. The techniques will be based on image data obtained
with the New Pol Scope described in a recent article in J. Microscopy,
Nov. 1995, Vol. 180, p. 140-147.

More information on the postdoc position can be obtained at the
Biophysical Society Employment Service:

http://biosci.cbs.umn.edu/biophys/employ.html

Look under "Positions", "Postdoc", "**Marine_Bio-MA-microscopy"
_____________________________________________________________
Rudolf Oldenbourg, PhD
Architectural Dynamics in Living Cells Program
Marine Biological Laboratory, Woods Hole, MA 02543, USA
Tel:508-289-7426, Fax:508-540-6902, E-mail:rudolfo-at-mbl.edu




From: Mark Wall :      Mark.Wall-at-quickmail.llnl.gov
Date: 22 Feb 1996 15:16:04 -0800
Subject: Frontiers Conference

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Message-ID: {n1387119776.33532-at-quickmail.llnl.gov}

Call for papers / exhibitors

6th Frontiers conference on electron microscopy in materials science
June 4-7th, Hyatt Regency Oak Brook Hotel, Oak Brook, IL, USA.
Sponsored by ANL, LLNL, LBL, UOP, ORNL, and LANL

This conference will provide an international forum on the appliction of
advanced EM techniques to problems in materials science. Plenary lectures will
summarize the principles and practices of specific techniques used in the
field. Additional presentations will give examples of applications.

Organizing committee: C. W. Allen ANL, W. E. King LLNL, T. E. Mitchel LANL, S.
A. Bradley UOP, T. Nolan ORNL, U. Dahmen LBL, M. Wall (exhibits) LLNL

Program Committee: Magnetic materials-R. Sinclair, Quantitative-HREM U.
Dahmen,
Orientation imaging-H. Weiland, Advanced techniques-S. Bradley, Energy
filtered imaging-J. Mayer.

Subject areas for solicited papers: Metals, HREM, XEDS, Ceramics, Electronic
materials, OIM, Surfaces, EELS, Magnetic materials, CBED, Holography, Specimen
preparation, etc.

For registration information contact Karen Sitzberger at LLNL, 510-423-7988

For exhibitor information contact Mark Wall at LLNL, 510-423-7162, or
Mark.Wall-at-quickmail.llnl.gov
(limited to 15-20 booths)





From: STEELE-at-KRDC.INT.ALCAN.CA
Date: Thu, 22 Feb 1996 13:32:20 -0500 (EST)
Subject: SURFACE WETTING MEASUREMENT

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--Boundary (ID Y4uc1wm7BTmhEsWCy+NgrQ)
Content-type: TEXT/PLAIN


Good day all.

I have a client who wishes to measure the volume of a liquid wetting a rough
surface.

The liquid fills the "troughs" in the surface, but due to surface tension,
overfills them.

Can anyone suggest a suitable technique for making this measurement?

I've been thinking along the lines of a focus calibrated scanning stage
optical technique (laser confocal?), but this is by no means my area, and I'm
not sure the instrument exists to do the job.

Any suggestions of optical, electron optical, or scanning probe techniques
would be appreciated.

Please respond to the server, or directly to:

###############################################################
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# Don Steele STEELE-at-KRDC.INT.ALCAN.CA #
# ALCAN INTERNATIONAL #
# Kingston Research and Development Center #
# P. O. Box 8400 #
# Kingston, Ontario Canada K7L 5L9 #
# #
###############################################################



--Boundary (ID Y4uc1wm7BTmhEsWCy+NgrQ)--




From: chender-at-umich.edu (Carl Henderson)
Date: Thu, 22 Feb 1996 15:14:22 -0500
Subject: Re: EDX sum peak

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Mark Darus writes:}
} I'm looking at fused silica, quartz, trying to identify an inclusion
} which I think is iron. I have a good K alpha iron peak, so all is well so
} far, but also I have a large peak in an area where either Al or a sum peak
} for the L alpha line of iron should be, but I have no peak at the area
} where the L alpha should be.
} My question is, can you have a sum peak, on EDX, for a particular
} element, and not have a peak where the one should be that is causing the
} sum peak?
} I think the answer is yes, perhaps my counts are coming in too fast
} and its doubling everything and showing a sum peak, but I thought I would ask.

You should also see an Fe L-alpha peak if you are seeing a sum peak for Fe
L-alpha, *unless* the lower level discriminator on your EDS system is set
so high as to cut off low energy lines.

If you suspect that the detector window is absorbing the Fe L-alpha lines,
that detector would still absorb the two photons arriving simultaneously to
create a sum peak, since each photon still only has the energy of the Fe
L-alpha x-ray line.

Lowering the accelerating voltage below the critical excitation voltage for
Fe K-alpha does not necessarily eliminate the possibility of a Fe L-alpha
sum peak since the Fe L-alpha line will still be generated.

The best way to decide if it is a Fe L-alpha sum peak or not is to lower
the x-ray count rate to reduce the incidence of two photons arriving at the
same time. A simple tweaking of the beam current using a condensor lens
control will do this.

(Of course an even better method would be to make the observations with a
wavelength dispersive spectrometer where sum peaks are not a problem.)

Good luck,

Carl


======================================
Carl Henderson
University of Michigan
Electron Microbeam Analysis Laboratory
2501 C.C. Little Bldg.
Ann Arbor, MI 48109-1063






From: Dr Martin Hoppe :      mhoppe-at-llt.de
Date: Fri, 23 Feb 1996 11:12:08 +-100
Subject: Unsubscribe

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Please unsubscribe me from the list.





From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Fri, 23 Feb 1996 09:29:48 EST
Subject: acrylic solvent

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In response to Randy Nessler's query on solvents for acrylic-type resins,
methylene chloride works very well. This solvent can be used to
completely solubilize Plexiglas and other acrylic polymers. It is a
principal ingredient in commercial paint strippers.

-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: hagler.herb-at-pathology.swmed.edu (Herb Hagler, Ph.D.)
Date: Fri, 23 Feb 1996 08:38:13 -0600
Subject: Re: Calculating Area

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Martin,
The use of a point counting grid for measuring areas is certainly a 1990's
approach and one advocated by the 'new stereology' group in Europe. Point
counting is the most efficient use of time to get excellent estimates and
takes less time than using thresholding or tracing of individual muscle
fibers. If you combine NIH Image with some of the point counting macros on
the NIH Image server you can capture video images of your tissue, overlay a
point counting grid, score the hits within NIH image and export the data to
excell. In general the maximum number of points that are needed for a good
estimate is between 50 to 100. This combined with proper sampling of your
tissue will yield quick and accurate results. See some of the more recent
papers by Gunderson and group. You will find that this technique gives a
more precise answer than the methods of planimetry or thresholding an
image.

} I would like to calculate the individual areas of muscle fibers in frozen
} muscle sections. In the past I have used a planimeter and/or a simple grid
} transparency. I would like to switch to a more "1990's" method, presumably
} using a graphics tablet and the appropriate software. Basically, the
} software would not only have to calculate individual fiber areas, but also
} what percent of the entire muscle is made up of one fiber type or another.
} Certainly a built in statistical package would be useful. If anyone could
} recommend a set-up for use with a Macintosh, I would greatly appreciate it.
} If there is another way besides a graphics tablet, I would certainly
} consider that as well.
}
} Thank you,
}
} Martin A. Levin
} Department of Biology
} Eastern Connecticut State University
} Willimantic, CT 06226
} Phone: (860)465-4324 FAX: (860)465-5213

Herb Hagler, Ph.D.
Director of Computer-Assisted Instruction
for Southwestern Medical School
UT Southwestern Medical Center
(214)648-3890 Fax(214)648-3925






From: Liang, Long :      LLIANG-at-is.arco.com
Date: 23 Feb 1996 10:36:10 CST
Subject: SEM + laser Raman ?

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Message-Id: {MACMS.LLIANG.244134100096054FMACMS-at-IS.ARCO.COM}

Dear Microscopists,

I read an article published many years age. It mentioned that
techniques such as laser-raman spectrometry coupled with electron
microscopy can offer a chance to identify molecule structures of "in
situ" organic matter. Does anyone know of such kind of instrument
available ? Thanks.

Long Liang
ARCO EPMA/SEM Lab
Plano, TX






From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Fri, 23 Feb 1996 12:10:36 -0500 (EST)
Subject: external computer drives

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Hi all,

A fellow researcher is interested in purschasing either a SyQuest drive or a
Zip drive. Are there any of you kind folks out there that have either/both.
Any comments regarding either system will be much welcomed.

If this subject has been discussed before, please accept my apoligies(sp).

Thanks,
Ed Calomeni
Dept Pathology
Medical College of Ohio
Toledo, OH 43699
emlab-at-opus.mco.edu





From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Fri, 23 Feb 1996 12:10:36 -0500 (EST)
Subject: external computer drives

Contents Retrieved from Microscopy Listserver Archives
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Hi all,

A fellow researcher is interested in purschasing either a SyQuest drive or a
Zip drive. Are there any of you kind folks out there that have either/both.
Any comments regarding either system will be much welcomed.

If this subject has been discussed before, please accept my apoligies(sp).

Thanks,
Ed Calomeni
Dept Pathology
Medical College of Ohio
Toledo, OH 43699
emlab-at-opus.mco.edu





From: STEELE-at-KRDC.INT.ALCAN.CA
Date: Fri, 23 Feb 1996 11:14:20 -0500 (EST)
Subject: SURFACE WETTING

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--Boundary (ID BzdtUN6SoF3ZW4c6OeK2Nw)
Content-type: TEXT/PLAIN

Thanks to those who have responded to my request for technique suggestions
for the measurement of a liquid wetting a rough surface.

I'm afraid "volume" was perhaps a poor choice of words on my part for the
measurement I wish to make. I'm also interested in knowing how far above the
nominal surface the wetting liquid is (due to surface tension), and hopefully
wish to map this over a defined area. I'm expecting the cross section to be
something like:
___
_/ \
liq. __/ /\ \_ /
/\ / \ /
substr. _/ \/ \__/


What do you think about a profilometer trace over a frozen sample, followed
by a trace over the same, cleaned area?

###############################################################
# #
# Don Steele STEELE-at-KRDC.INT.ALCAN.CA #
# ALCAN INTERNATIONAL #
# Kingston Research and Development Center #
# P. O. Box 8400 #
# Kingston, Ontario Canada K7L 5L9 #
# #
###############################################################



--Boundary (ID BzdtUN6SoF3ZW4c6OeK2Nw)--




From: ldettin-at-cmefcm.uncor.edu (Luis Ernesto Dettin)
Date: Fri, 23 Feb 1996 16:54:21 -0500
Subject: SURFACE WETTING

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please, subscribe me. thanks!
Dr. Luis E. Dettin
ldettin-at-cmefcm.uncor.edu
Centro de Microscopia Electronica
Facultad de Cs Medicas-UNC
Te/Fax: 01-051-690442
CC 362
5000 Cordoba
Argentina





From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 23 Feb 96 15:27:14 EST
Subject: external computer drives

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Message-id: {25589273-at-dancer.Dartmouth.EDU}

The 100 meg Zip drive from Iomege is terrific, you can carry an enormous
amount of information, including particularly greedy TIFF images, in your
pocket.
Kate Connolly




From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 2/22/96 4:18 PM
Subject: Dissolving polymerized acrylic?

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Hello Everyone,
I need to find out what solvent can be used to dissolve polymerized LR
White or Lowicryl. I am not sure which resin is the culprit. We have a
Reichert-Jung CS auto cryo-substitution unit, whose specimen substitution
chamber has become stuck in the substitution container (terminology taken from
the owners manual). Since it has been stuck for some time, and I wasn't
informed of the resin being used at the time the problem started, I can only
limit it to LR White or Lowicryl. A vendor of LR White suggested that 'acrylic
paint remover' might be the only hope. What common lab chemicals are in
'acrylic
paint remover'. Any other suggestions?
Thanks in advance,


Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu






From: Matt Boettner :      boettner-at-Spacestar.COM
Date: Fri, 23 Feb 1996 17:35:01 -0600
Subject: Dissolving polymerized acrylic?

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To all confocal and optical microscope users:

Kovex corporation is currently developing a low-cost confocal
microscope system that will provide real-time 3D images on a PC based
platform. The system eliminates the use of an optical microscope in its
design and is completely portable.

We are interested in receiving feedback from users and purchasers of
microscope equipment in order to determine the usefulness of our products.
We currently use a fixed wavelength system that reduces the flexibility of
our system for the fluorescence user, but provides more opportunities for
cost reduction to the user of reflectance microscopy. A complete system
including software, PC, and external microscope apparatus will sell for
$68,000. It is our intention to make this technology more accessible for
use in quality control environments, and in industrial surface analysis. We
believe our system will be used as a primary analytical tool in R&D
environments where optical systems currently dominate.

Any feedback will be greatly appreciated.

Thank you,

Matthew C. Boettner
President and CEO
Kovex Corporation

E-mail: boettner-at-spacestar.com
Voice-at- (612) 730-0468
Pager-at- (612) 530-1399





From: Paul Webster :      paul.webster-at-yale.edu
Date: 23 Feb 1996 16:23:55 -0500
Subject: Re: Calculating Area

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Message-Id: {n1387029703.18745-at-QuickMail.Yale.edu}

Re} Martin Levin
} I would like to calculate the individual areas of muscle fibers in frozen
} muscle sections. In the past I have used a planimeter and/or a simple grid
} transparency.

I agree with the reply given by Herb Hagler, the overlay counting methods are
efficient, easy to use, and belong to the 1990's battery of techniques. They
can be easily used on digitized images too.

However, I would like to add an additional point. If you are interested in
individual fiber areas then working with the selected fibers is OK. If you are
more interested in the mean fiber volume then you must take care to properly
sample the whole population. Working on a few selected pictures or tracings will
add a bias to your result and thus disqualify it.

To properly eliminate bias requires a defined sampling strategy which starts at
the animal (or culture dish) level and continues through embedding and
sectioning to photography. One paper to cover this point in a readable way is in
J. Histochem. Cytochem. 40:1929-1936 1992 and is written by John Lucocq.

Best regards,
Paul Webster, Ph.D.
Center for Cell Imaging,
Yale School of Medicine.





From: chender-at-umich.edu
Date: 23 February 1996 10:07
Subject: Re: EDX sum peak

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microscopy-at-Sparc5.Microscopy.Com, chender-at-umich.edu



Mark,
You do not say if you have a detector capable of detecting low energy=20
( {1keV) x-rays. If you do not then the peak cannot be an Fe L sum peak as=20
the Fe L x-ray will not reach the detector crystal and therefore cannot the=
n=20
cannot create signals which can contribute to the sum peak .
Eric
----------

{ {File Attachment: UUCP_ENV.TXT} }
Mark Darus writes:}
} I'm looking at fused silica, quartz, trying to identify an=20
inclusion
} which I think is iron. I have a good K alpha iron peak, so all is well so
} far, but also I have a large peak in an area where either Al or a sum peak
} for the L alpha line of iron should be, but I have no peak at the area
} where the L alpha should be.
} My question is, can you have a sum peak, on EDX, for a particular
} element, and not have a peak where the one should be that is causing the
} sum peak?
} I think the answer is yes, perhaps my counts are coming in too fas=
t
} and its doubling everything and showing a sum peak, but I thought I would
ask.

You should also see an Fe L-alpha peak if you are seeing a sum peak for Fe
L-alpha, *unless* the lower level discriminator on your EDS system is set
so high as to cut off low energy lines.

If you suspect that the detector window is absorbing the Fe L-alpha lines,
that detector would still absorb the two photons arriving simultaneously to
create a sum peak, since each photon still only has the energy of the Fe
L-alpha x-ray line.

Lowering the accelerating voltage below the critical excitation voltage for
Fe K-alpha does not necessarily eliminate the possibility of a Fe L-alpha
sum peak since the Fe L-alpha line will still be generated.

The best way to decide if it is a Fe L-alpha sum peak or not is to lower
the x-ray count rate to reduce the incidence of two photons arriving at the
same time. A simple tweaking of the beam current using a condensor lens
control will do this.

(Of course an even better method would be to make the observations with a
wavelength dispersive spectrometer where sum peaks are not a problem.)

Good luck,

Carl


ffffffffffff=3DCarl Henderson
University of Michigan
Electron Microbeam Analysis Laboratory
2501 C.C. Little Bldg.
Ann Arbor, MI 48109-1063







From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Fri, 23 Feb 1996 19:35:34 -0600
Subject: LM: fluorescence filter for Leica

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I am trying to locate a Leica microscope dealer for info on a slide-in
filter to remove the endogenous fluorescence produced by chloroplasts when
using a Rhodamine filter-pak.

Anyone have any info on the characteristics of such a filter? On another
brand of microscope, such a filter is numbered 575DF40 and 9336. I assume
the 575 refers to the wavelength allowed to pass.

Thank you.

#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Phil Elizondo :      PELIZONDO-at-svc.com
Date: Fri, 23 Feb 1996 16:24:07 PST
Subject: SEM Analysis Engineer Opening

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Immediate Opening for SEM Analysis Engineer at SVC:


Principal Duties & Responsibilities:

SEM analysis of display components; basic failure analysis.
Responsible for upkeep of JSM 820, with full JEOL service contract;
Supervision & training of other SEM users.



Special work circumstances:

It is preferred that candidate be able to work either an early (start
-at- 6AM) or late (finish -at- 8PM) shift.



Experience & Education Requirements:

B.S. in Physical Science or Engineering or equivalent.
Minimum 3 yrs. experience in Electron Microscopy.


Company Background:

In just 4 years Silicon Video Corp. has become one of the hottest
start ups in Silicon Valley. We are developing a new class of flat
panel display - Thin CRT. Millions in funding from the government
have been obtained. Large corporate partners in industry and
academia connections have been secured. We're searching for highly
qualified candidates to join our team.

Please send resume response to both,

pelizondo-at-svc.com
dcaraway-at-svc.com





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Fri, 23 Feb 1996 16:49:41 -0600
Subject: Re: external computer drives

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QUESTION:
}
} A fellow researcher is interested in purschasing either a SyQuest drive or a
} Zip drive. Are there any of you kind folks out there that have either/both.
} Any comments regarding either system will be much welcomed.
}
} If this subject has been discussed before, please accept my apoligies(sp).
}
} Ed Calomeni

RESPONSE:

I use both. I have far more experience with SyQuest. No problems with SQ
ever. I tend to favor SyQuest because the head is never in contact with the
platter which is rigid and substantial. SyQuest has a tremendously larger
user base and they are the standard in the publishing industry. There are
presently more of the larger (5.25") SQ platters than the 3.5" SQ's. ZIP
drives use a floppy medium which is held away from the heads by air flow.
Speed wise they are both similar. Replacement platters (disks) are
considerably cheaper with ZIP drives - about half. So, it dependends on
whether price is an issue or if ready exchange of disks is the concern.
ZIP's are new and so we don't know yet if they will be as reliable as SQ's.
Costs for the drives are similar ($200 for the 100 MG drives). You know,
the ZIP folks, IOMEGA, have now come out with the JAZ drive, a 1 GB disk
that is very cheap and the drive costs $400-500.





#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: bob-at-befvax.uchicago.edu
Date: Fri, 23 Feb 1996 17:28:22 EST
Subject: Re: external computer drives

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Postdoctoral Position Available at the University of Chicago

in the following areas of research


} RED CELL CYTOSKELETON

} Red cells are able to elastically deform. The extraordinary
} importance of this property can be appreciated from recalling that,
} although the human red cell is a biconcave disk eight microns in
} diameter, it can easily pass through capillaries half this size.
} This remarkable feat is accomplished by the red cell transiently changing
} its shape during passage after which its well-known biconcave
} form spontaneously and nearly instantly recovers. We are studying
} the structural basis of this property using cryoelectron microscopy.
}
} McGough, A. and Josephs, R. (1990) On the structure of
} erythrocyte spectrin in partially expanded skeletons. Proc. Notl.
} Acad. Sci. USA 87: 5208-5212.
}
} Li Li and Robert Josephs. Cryo-Electron Microscopy of Human
} Erythrocyte Membrane Skeletons. Proceedings of the 51st annual
} meeting of the Microscopy Society of America San Francisco
} Press, San Francisco. p. 116 (1993).
}
} SICKLE CELL HEMOGLOBIN

} Sickle cell anemia is caused by the intracellular polymerization of
} sickle cell hemoglobin to form rod-like fibers. A knowledge of the
} fiber structure could be used for the design of an agent that could
} block fiber formation. We have combined the structure of hemoglobin
} (known from X-ray crystallography) and the molecular coordinates
} of the fiber (determined from electron microscopy) in order to
} synthesize a model which shows the intermolecular contacts of the fiber.
} This approach has allowed us to determine the contacts which form
} between molecules in the fiber. We are now studying how various mutations
} (some obtained by site directed mutagenesis) affect fiber structure.
} Such studies are expected to account for the structural and chemical
} properties of fibers in terms of intermolecular interactions.
}
} Watowich, S., Gross L., and Josephs, R. (1989) Intermolecular
} Contacts within Sickle Hemoglobin Fibers. J. Mol. Biol. 209: 821-828.
}
} M. R. Lewis, L. J. Gross, and R. Josephs. Variable Pitch in Frozen
} Hydrated Sickle Hemoglobin Fibers: An Image Analysis Model
} Study. Ultramicroscopy, 56 303-317 (1994).
}
} Michael R. Lewis, Leon J. Gross, and Robert Josephs. Cryo-
} Electron Microscopy ofDeoxy-Sickle Hemoglobin Fibers.
} Microscopy Research and Technique 27 459 - 467 (1994).
}
}
} ACETYLCHOLINE RECEPTOR
}
} The acetylcholine receptor is responsible for transduction of the nerve
} impulse to muscular contraction. The receptor lies at the neuromuscular
} junction in the muscle membrane. It has five subunits denoted as
} a1,a2,b,d,g. Current work in our lab involves labeling different
} subunits with monoclonal antibodies against defined defined sequences
} in order to determine the subunit arrangement (which appears to
} still be a matter of debate in spite of the considerable
} work done in other labs). We are using two dimensional crystalline
} tubes in order to determine the location of the labels.


} Interested individuals please contact

} E-mail: Bob-at-befvax.Uchicago.Edu
}
} } Robert Josephs
} The University of Chicago
} 920 East 58th Street
} Chicago, IL 60637
}





From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Sat, 24 Feb 1996 09:53:28 +0000 (GMT)
Subject: Re: Electron Beam cleaning

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I am by no means an expert in this field but I understand there is a
process known as Electron Beam energy filtering. You might try this as it
could take out all the nasty bits and may clean the electron beam.Patrick
Echlin
Cambridge UKOn Tue, 20 Feb 1996, L.D.Marks wrote:

} I have been persuaded (now to my regret) to give a talk about
} electron beam cleaning. Having posted requests for information to a number
} of logical sources (e.g. the surface science listserver) and only recieved
} information requesting that I send THEM anything useful, let me try here -
} at least we use electron beams! Has anyone heard of this and know any
} useful references or links ?
}




From: John M. Libert :      jlibert-at-cpcug.org
Date: Sun, 25 Feb 1996 06:54:28 -0500
Subject: Re: external computer drives

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Message-Id: {9602241702.AA09878-at-carbon60.fysik.dtu.dk}

EmLab wrote:
}
} Hi all,
}
} A fellow researcher is interested in purschasing either a SyQuest drive or a
} Zip drive. Are there any of you kind folks out there that have either/both.
} Any comments regarding either system will be much welcomed.
}
} If this subject has been discussed before, please accept my apoligies(sp).
}
} Thanks,
} Ed Calomeni
} Dept Pathology
} Medical College of Ohio
} Toledo, OH 43699
} emlab-at-opus.mco.edu

Syquest drives are fine, but media will cost about the same as for
rewritable magneto-optical media ($200 / cartridge). Syquest, of course,
is comparatively lower priced than magneto-optical. However, for serious
image storage, Pinnacle Micro's 4.6 GByte M/O drive is great! At around
$1700 it is more than a Syquest, but when one considers the cost of
media, may actually be quite economical. I will send you additional
information via mail.

John Libert




From: John M. Libert :      jlibert-at-cpcug.org
Date: Sun, 25 Feb 1996 07:05:27 -0500
Subject: Re: external computer drives

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Message-ID: {31305087.4F26-at-cpcug.org}

EmLab wrote:
}
} Hi all,
}
} A fellow researcher is interested in purschasing either a SyQuest drive or a
} Zip drive. Are there any of you kind folks out there that have either/both.
} Any comments regarding either system will be much welcomed.
}
} If this subject has been discussed before, please accept my apoligies(sp).
}
} Thanks,
} Ed Calomeni
} Dept Pathology
} Medical College of Ohio
} Toledo, OH 43699
} emlab-at-opus.mco.edu

Regarding my last message about Pinnacle Micro magneto-optical drives,
you can check out Pinnacle at http://www.pinnaclemicro.com.

John Libert




From: :      VFN6T-at-DMT03.mcc.Virginia.EDU
Date: 25 Feb 96 10:58:10 EST
Subject: Immunohistochemistry in plastic

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Dear Colleagues,
I am using plastic-embedded (Lowicryl or JB-4) sections of fetal kidneys
and would like to use standard avidin-biotin-peroxidase techniques for
immunohistochemistry. To date, we are unable to achieve specific staining.
We have tried two different etching protocols and a few of the JB-4 (but none
of the Lowicryl) sections survive, but still no staining. Most references
give little information regarding incubation times, etc. Can anyone help me
trouble-shoot or point me towards a good reference? Thanks in advance.
Victoria F. Norwood, M.D.
Department of Pediatrics
University of Virginia
vfn6t-at-dmt03.mcc.virginia.edu





From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 25 Feb 1996 11:46:34 -0600
Subject: 1996 Meeting -Call for Papers Now On-Line !

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G'day Subscribers...

The Call for Papers and Instructions for Manuscript
Submission is now (~90%) on-line on the MSA WWW site.

You may download/access the information at:

http://www.msa.microscopy.com

Look for the hotlink called "Registration & Meeting Info."

There are a few forms that still need processing, however,
they will be on-line in a few more days.

If you are still waiting for your HardCopy of the Call for
Papers this information will allow you to get started
on your Manuscript preparation. All the information
in the HardCopy Booklet (except for some artwork) will
be reproduced here.

--------------------------------------------------------

Several new options have been also added to the MSA WWW Site.

1.) New Search Engine Access

A new options for direct connection to WWW Search Engines
has been implemented. Just enter your search criteria and
choose your favorite search engine.

2.) New Aliases to Society Office Holders

You may directly Email the office holders of MSA
by using the aliases shown below.
Direct links are also provided on the MSA WWW Page.


President (MSAPresident-at-MSA.Microscopy.Com) includes Past-President &
President-Elect

Secretary (MSASecretary-at-MSA.Microscopy.Com)

Treasurer (MSATreasurer-at-MSA.Microscopy.Com)

Council (MSACouncil-at-MSA.Microscopy.Com) reaches all current Council
Members

Business Office (BusinessOffice-at-MSA.Microscopy.Com) direct to the Business
Office

WebMaster (I'll let you figure out this one)

These links are aliases and provide you with a method to always reach the
current office holder.

Cheers..

Nestor
Your Friendly Neighborhood SysOp






From: levin-at-ecsuc.ctstateu.edu (Martin Levin)
Date: Sun, 25 Feb 1996 13:08:46 -0600
Subject: Re: external computer drives

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Ed,
I have the SyQuest EZ135 at home. I've had no problems with it at
all. Access time is fast (the manufacturer claims it is significantly
faster than the Zip drive). As a matter of fact, its so fast that I use it
as a working hard drive. The 135 Mb cartridges are about $23.00 each (less
if you buy in bulk). It is also quite small; about the size of an external
CD ROM drive. I have never used a ZIP drive, so I can't make any real
comparisons. I can only assure you that the EZ135 is a great little drive.


Marty

PS I don't work for or have stock in SyQuest.



} Hi all,
}
} A fellow researcher is interested in purschasing either a SyQuest drive or a
} Zip drive. Are there any of you kind folks out there that have either/both.
} Any comments regarding either system will be much welcomed.
}
} If this subject has been discussed before, please accept my apoligies(sp).
}
} Thanks,
} Ed Calomeni
} Dept Pathology
} Medical College of Ohio
} Toledo, OH 43699
} emlab-at-opus.mco.edu

Martin A. Levin
Department of Biology
Eastern Connecticut State University
Willimantic, CT 06226
Phone: (860)465-4324 FAX: (860)465-5213






From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Sun, 25 Feb 1996 15:00:03 -0600
Subject: Re: Dissolving polymerized acrylic?

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Message-Id: {v02120d00ad567dbd6bc7-at-[128.174.107.205]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

From Bob Citron:
} Hi Randy;
}
} I would suggest using methylene chloride. This works best for our PMMA
} (acrylic)lens products.
}
We have the same problem. The trouble with suggesting solvents like
MeChloride, Chloroform, or other such things is that the R.-J.
cryo-substitution unit is a good-sized piece of floor-occuping equipment,
and can't be put in a fume hood. I wouldn't particularly want to be in a
lab full of (e.g.) MeChloride fumes; we're exposed to enough toxins and
carcinogens as it is.
Any less noxious ideas?
Phil Oshel

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: allardlfjr-at-ornl.gov (Larry Allard)
Date: Sun, 25 Feb 1996 17:26:37 -0500
Subject: external computer drives

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X-Sender: l2a-at-cosmail1.ctd.ornl.gov
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Ed:

Regarding which removable storage drive to choose: be advised that Iomega
Zips are outselling EZs all across the country by a large margin, and also
that every EZ drive sold by Syquest *loses* a significant sum ($50-$100)
for the company. Syquest recently announced such poor earnings (a loss of
about $3.00 per share) that they have *laid off 60% of their world wide
work force*. The media used for the Syquest cartridges is defective 270MB
disks (one side is used) and has *no chance* of becoming the next
"standard" which replaces the 1.4MB floppy, while there is an excellent
chance that the Zip 100MB disk *will* become the new standard. It is
expected by many that Syquest cannot continue to bleed money if they are to
remain a viable company, and may have to abandon the EZ as a competitive
product.

Our laboratory has adopted Zips almost universally (I know of only 1 EZ
locally), and to date all of our external users are following suit. Among
a 7 member group, we have 20 or more Zips either in the lab, our offices,
or at home. Check your local retailers (Circuit City, CompUSA, etc.) and
see what is selling. I think you will find that if you purchase a Syquest
EZ, you will become a member of a small minority, and will run the risk
that you have an obsolete drive that uses media which will not be
compatible with the future standard (whatever it may be). Of course, you
only risk a couple of hundred bucks, and 20 bucks per cartridge, so it is
not a large risk.....


good luck.

Larry
PS as I have posted here before, I *do* own (and continue to recommend)
Iomega stock, so do your own homework.... ;-)







From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Sun, 25 Feb 1996 17:05:05 -0600
Subject: Re: Immunohistochemistry in plastic

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Message-Id: {v01520d00ad569a3de6d2-at-[128.206.15.200]}
Mime-Version: 1.0
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} Dear Victoria,
Many antigens can be stained in JB-4 or Lowicryl without
etching of any kind. Many etching procedures are bad for antigenicity, so
you might try seeing what you get without etching at all.

And, you did not say if you were amining for light microscopy or
em. But if you are working in light microscopy and you find that you are
unable to get good staining from Lowicryl or JB-4, you might try the
removable methacrylate system. This is a mixture of methacryates without
crosslinker, so you can remove the resin nearly completely with a 10 min
incubation in acetone. We have had very nice results with this for
immunocytochemistry at the light level. If you are interested, I can
provide more details.

Cheers,
Tobias

} I am using plastic-embedded (Lowicryl or JB-4) sections of fetal kidneys
} and would like to use standard avidin-biotin-peroxidase techniques for
} immunohistochemistry. To date, we are unable to achieve specific staining.
} We have tried two different etching protocols and a few of the JB-4 (but none
} of the Lowicryl) sections survive, but still no staining. Most references
} give little information regarding incubation times, etc. Can anyone help me
} trouble-shoot or point me towards a good reference? Thanks in advance.


- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Sun, 25 Feb 1996 20:52:11 -0600
Subject: External drives

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Message-Id: {v02120d01ad56d00eb1c8-at-[128.174.23.240]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Allow me to add my voice to the chorus for Zip drives. I use one at
home, and it works well. Two points I haven't seen mentioned: the 100MB
discs aren't much larger (mostly thicker) than regular floppies, but are
rugged, and the drive itself is small enough to take with you if you
travel. Take the Zip Tools & Install discs with the drive & you can use
your Zip anywhere others have the same computer as you (Mac or DOS).
Phil Oshel

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: Giorgio :      giocar-at-risc990.bologna.enea.it
Date: Mon, 26 Feb 1996 10:41:43 +0100
Subject: need books on biology lab techniques

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I'm looking for titres of books on BIOLOGY LABORATORY TECHNIQUES.
I opened a site for amateur scientists www.best.com/~funsci
and I need information about experimental techniques in biology,
microscopy, etc... suitables for scool or research applications.
Can you help me?
Please, send the answers here: funsci-at-best.com
Thanks George





From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Mon, 26 Feb 1996 08:34:22 -0500
Subject: Storage Media

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In the discussion of removable media I have not read anything about
"storage life" of data. Does anyone know about predicted life for
data/images stored on Syquest, Zip, CD, or magnetic tape cartridges? Will
all of these media require re-copying every five years to ensure data
integrity? That also begs the question as whether of any of these drives
will around in five years to read them.

David Rothbard

--
Institute of Paper Science and Technology






From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Mon, 26 Feb 1996 09:27:12 -0600
Subject: Re: external computer drives

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Can't answer your question exactly. We have an internal Syquest drive and it
is rather old (3-years). We have come to question its reliability because
two of the drives had to be reformatted after about six months of use.

I have been pleased with the IOMEGA products. We have some older Bernoulli
drives around. One of the five drives we have apparently developed a sticky
mechanism after about 5 years, but otherwise has been good.

} A fellow researcher is interested in purschasing either a SyQuest drive or a
} Zip drive. Are there any of you kind folks out there that have either/both.
} Any comments regarding either system will be much welcomed.
}
} If this subject has been discussed before, please accept my apoligies(sp).
}
} Thanks,
} Ed Calomeni
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Mon, 26 Feb 1996 08:34:22 -0500
Subject: Storage Media

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From my reading it seems that magnetic media has an archival lifespan of about 10 years whereas data
archived on CD-ROM has an archival lifespan of 30 years. Since I plan on retiring in 25 years, my bet is to
archive on CD-ROM and not have to repeat the archival process every 8-10 years.

The upside of magnetic media is the speed at which you can archive I guess, the latest CD-R drives appear to
need about 40minutes to "burn" the data on disk.


Kevin McCarthy


Regarding lifespan of magnetic media, David Rothbard wrote:


In the discussion of removable media I have not read anything about
"storage life" of data. Does anyone know about predicted life for
data/images stored on Syquest, Zip, CD, or magnetic tape cartridges? Will
all of these media require re-copying every five years to ensure data
integrity? That also begs the question as whether of any of these drives
will around in five years to read them.

David Rothbard

--
Institute of Paper Science and Technology


Kevin McCarthy
Assistant Professor
Department of Cell Biology
Digital Imaging Microscopy Facility
University of Alabama at Birmingham
Birmingham, Alabama 35294
Phone 205-934-9923/9924
Fax 205-934-7029
"Seeing the World Through Different Eyes"




From: Beverly E Maleeff
Date: 26 Feb 96 13:58:16 EDT
Subject: Re: ISO9000 Certification

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FYI:
The MSA Technologists' Forum is sponsoring a Roundtable discussion at this
year's Microscopy & Microanalysis meeting in Minneapolis. The theme of the
roundtable is "Coping with Regulations: ISO and OSHA". As the session chair,
I'm glad to see this thread on the Listserver. I encourage everyone with an
interest in how regulations govern our lives in microscopy labs to attend this
session. Further details will be available prior to the meeting.

Regards,
Bev Maleeff
SmithKline Beecham Pharmaceuticals
Toxicology-US, UE 0462
709 Swedeland Road
King of Prussia, PA 19406
610/270-7987
610/270-7202 fax
Beverly_E_Maleeff-at-sbphrd.com ***note the new e-mail address***





From: Paul Webster :      paul.webster-at-yale.edu
Date: 26 Feb 1996 12:27:11 -0500
Subject: Re: calculating area

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Message-Id: {n1386784506.66376-at-QuickMail.Yale.edu}

Don Chernoff writes
"Could someone explain how the overlay counting methods can give areas of
individual fibers?"

Cross-lattice overlays (see Weibel, E. R. 1979. Steeological methods, vol 1.
Practical methods for biological morphometry. Academic Press, New York.) are
transparent sheets which have grids (or lines) of known dimensions on them. A
simple overlay would be a square grid. For area counts, the corner of the grid
is taken to represent the whole square. Therefore if the points over the
structure to be sampled are counted this is the same as counting the number of
squares. On samples of properly randomized specimens, this is a very accurate
way of estimating area.

Since the sections that are sampled have a thickness, the area can also be used
to estimate volumes.

The test lines can also be used to accurately estimate length and thus surface
area.

Only small numbers of micrographs are required to do these analyses, counting
(unlike tracing) does not need to be accurate, and estimations can be rapidly
performed.







From: Walt Bobrowski (313) 996-7814 :      bobroww-at-aa.wl.com
Date: Mon, 26 Feb 1996 12:29:11 -0400 (EDT)
Subject: Re: Mass Storage Media

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All interested persons in removable, mass storage medias should check out the
March issue of PC World. It includes a very comprehensive article on this very
topic including speed, storage capacity, durability, archival quality, etc.

Walt Bobrowski
Parke-Davis Research






From: Walt Bobrowski (313) 996-7814 :      bobroww-at-aa.wl.com
Date: Mon, 26 Feb 1996 12:29:11 -0400 (EDT)
Subject: Re: Mass Storage Media

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All interested persons in removable, mass storage medias should check out the
March issue of PC World. It includes a very comprehensive article on this very
topic including speed, storage capacity, durability, archival quality, etc.

Walt Bobrowski
Parke-Davis Research






From: agoldsch-at-tamarugo.cec.uchile.cl (GOLDSCHMIDT DE LA MATTA ALFONSO)
Date: Mon, 26 Feb 1996 18:28:37 +0400
Subject: who are working ,with CAMECA-46

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Hello
I need information about,condition of analysis , about voltage,current,time deat, size of beam in Quantitative analysis and calibration
For analysis , in alluvial-gold (free gold).
For analysis on silicates .
Anybodi are using X-PHI software ? i have problem, with K alpha line and "K"
kalium .
My name is Alfonso Goldschmidt i am working in the University of Chile
in the GEOLOGICAL DEPARTAMENT.
I am working with the old microprobe CAMECA 46 and i am try of restart
the use, of this machine.

Thanks very much .




From: BARBARA.HARTMAN-at-1773.220.SCHERING-PLOUGH.sprint.com
Date: Mon, 26 Feb 1996 16:46:47 -0500
Subject: EM of HCV protease

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I have been asked to do TEM and/or SEM on Hepatitis C virus protease. I
have a biology background that deals with tissues not crystals. They would
like to examine the crystal structure of this protease. I understand that
it does not form a pellet and does not separate out no matter how hard you
spin it. The concentration of the sample is approximately 1 mg/ml. Where
should I begin ???? Could I do an air dried sample for scanning ??

Thank You,

Barbara Hartman
Schering-Plough Research
E-Mail: Barbara.Hartman-at-Schering-Plough.sprint.com
Phone: 201-579-4343
Fax: 201-579-4211






From: allardlfjr-at-ornl.gov (Larry Allard)
Date: Mon, 26 Feb 1996 12:37:15 -0500
Subject: media lifetime

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I agree that magnetic media has a lifetime of 10 years or so, whereas
optical media is far longer, say 30 years. However, I don't believe I have
*ever* accessed original data (say negatives) for any useful purpose even 5
years after original recording. Magnetic media has an overwhelming
advantage in daily usage, because of its speed, portability (the frisbee
test), and competitive price of media and drives. However, for the
convenience of our users, in our lab we record archival data (TEM images
etc) at present on CD-ROMs, and provide either Zips or CDs to our users to
carry their data home. But I certainly do not worry about how I will read
that archived data 25-30 years from now, because there is no question that
many generations of drive devices will supplant anything that is currently
available. We no longer use enlargers to print our negatives, but have
scanners that handle transparencies to use if we want to make some new
prints. When the time comes, we will transfer our archived images and
other data to the next generation of storage devices.

BTW, recently I installed a 400K diskette containing data from May 1984
recorded from my old 128K Macintosh. My Powerbook Duo288c running MacWrite
II opened a MacWrite file with no problem, and a recent version of MacPaint
opened an old MacPaint file. Of course, I had absolutly *no* interest in
any of that data after nearly 12 years.

Larry






From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Mon, 26 Feb 1996 10:07:19 -0600
Subject: LM/removable methacrylate

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FAGERLAND.JANE-at-igate.pprd.abbott.com

Greetings,
I mentioned a removable methacylate system for immunocytochemistry
and several netters have asked me for more information. For this reason, I
am posting the reply publicly, in case others are interested as well.

The basic resin is simply a mixture of butyl and methyl
methacrylate (80% butyl). This was a resin much used in the em *before* the
invention of epoxies, and now generally abandoned. But with the upsurge of
immuno techniques at the light microscope level, several groups in the late
80's ressurected this simple resin system because it has the advantage of
being easily removed after sectioning by a brief rinse in an organic
solvent, such as acetone. Removablility is an advantage because you gain
great access for your antibody to your antigen. In this respect, the
removable methacylate system is like paraffin embedding; only with
methacrylate, the structural preservation of the sample is generally much
better than with wax. So, if you are going after sub-cellular detail, then
methacrylate could be the resin for you.

If this sounds interesting, here are some references:

Baskin et al 1992 Planta 187:405 - 403.
Gubler 1989 Cell Bio Int Rep 13:137-145.
Van de Kant et al 1988 Histochem J. 20, 335 - 340. (animal tissue).

A protocol where protein localizations and in situ RNA localizations are
done on alternate sections:
Kronenburger et al. 1993 Cell Biol Intern. 17: 1013-1021.

A few notes. We drive polymerization of the methacrylate with UV
light (a catalyst is added to the resin), and this is a free radical based
mechanism. We were only able to get this method to work reliably with plant
material by including 10 mM DTT in the resin. This seems to prevent
radicals from attacking the tissue but not to prevent them from doing their
polymerization thing.
Also, polymerization is inhibited by oxygen. In the reference to my
own work above, polymerization was done in flat molds in a box exposed to a
flow of nitrogen gas. We now do embedding in capsules, under normal air
with no problems.
Also, as far as practical details go, compared to what we published
in the Planta paper above, we have found that there is no need to put DTT
in the ethanol series for dehydration, and we can compress the entire
dehydration infiltration and embedding schedule to two days, rather than
six.

I hope the above helps. If anyone has questions about specific
steps or other details, please feel free to contact me.

Cheers,
Tobias



- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: vickie-at-MACC.WISC.EDU
Date: Mon, 26 Feb 1996 12:25:12 -0600
Subject: LM/removable methacrylate

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! ! ! ! ! ! ! ! BACK BY POPULAR DEMAND ! ! ! ! ! ! ! !


The Second Annual Symposium on

"Integrated Microscopy"

on

September 20 - 22, 1996

at

The Wisconsin Center
702 Langdon Street
Madison, WI

Program details will be posted at a later date. Information is also
available on our World Wide Web page:

http://www.bocklabs.wisc.edu/imr/imr.html

#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*
Presentations will focus on biological problems for which a combination of
microscopies [i.e. integrated microscopy] has been used. The speakers will
demonstrate by example the power, potential and limitations of various
microscopical techniques. The techniques which will be discussed include:
DIC, Confocal, Multi-Photon Excitation Imaging, SEM, TEM, Cryo specimen
Preparation.
#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*

********************************************************************************
Following the symposium, the IMR will be conducting a 2-day workshop (Sept.
23 and 24). We will be presenting lectures and provide "hands-on"
experience for the following techniques:
* 2-photon excitation imaging
* 4D DIC imaging
* Cryo-SEM
* High pressure freezing
* Reversible embeddment for SEM and TEM

Workshop attendence will be limited to 20 participants.

********************************************************************************

This year's symposium will be co-hosted by :

the Integrated Microscopy Resource (IMR)
University of Wisconsin, Madison, WI
an NIH Biomedical Research Resource

and

the Center for Light Microscopy Imaging and Biotechnology
Carnegie Mellon University, Pittsburg, PA
an NSF Science and Technology Center






From: paulc-at-arms.gps.caltech.edu (Paul K. Carpenter)
Date: Mon, 26 Feb 1996 16:35:12 -0800
Subject: Zip vs. Syquest (longish)

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Hi all,

In the analytical facility at Caltech we have used both the Syquest 44 Mb
and the Zip 100 Mb removable-cartridge units. I have also used a Syquest
270 Mb unit for a few days. My selection of these units has been based on
performance, speed, reliability, and cost of the media for outside users.

My observations (all these systems were used on primarily on Macintosh
systems, except as noted):

1. The Syquest 44 became an early standard among the Macintosh crowd, many
people had them and they were used to transfer all sorts of software, data,
digital images, etc. The MAS computer workshop sort of settled on this
format for about 2-4 years, and we could count on being able to transfer
things with our collegues. The units were nearly bulletproof with a few
exceptions. Cartridges formatted on your unit at home could potentially
have problems on another drive if the head alignment differed drastically.
I observed this fewer than 3 times in the 5 years I have used these units.
As far as reliability, I have two units that were on continuously for about
5 years with no problems. The units are loud and noisy, but have speeds
rivaling older hard drives. The 44 Mb format is essentially history now,
although many have $$ invested in cartridges (I have 45 of them...being
consolidated onto Zip 100's now). There have been other formats available
(88Mb, 105Mb, 200Mb, 270Mb), and I think the 270 Mb format is the only one
that has achieved any kind of "standard" status.

Which reminds me to comment on the "longevity of media xyz". I think that
currently the technology is evolving more rapidly than the useful life of
any given media. For example, I have some Apple II disks with some
important stuff on them, but there are no Apple II's around here...so even
though the media is still (probably) ok, I don't have access to the devices
used to read/write this information. Same goes for all the DEC floppy
disks, RL02 platters, TK50 tapes...etc. The bottom line is that we now
have the obligation to periodically transfer *all* our important archived
data to the current "standard" which (probably) won't be in use 10 years
from now. The question "will the data on a floppy be readable 25 years
from now" is just plain academic, because those floppy drives will be found
only in the Smithsonian. The worst part is that by the time you *realize*
that you have archaic media, you find that it is obsolete in the sense that
you cannot find a system to read it on. And as the capacity of media ever
increases, so does the possiblity of losing really large sets of data. But
this is a discussion best held over a few beers, I guess :)

2. The Syquest 270 is nice, but I found the unit to be very noisy, and the
ejection lever felt as if it was going to break off every time I used it.
The quality level is not what I would expect (but bear in mind this is a S
270 mechanism in some vendors box, so the packaging quality and noise level
may vary by manufacturer). I had an insurmountable level of difficulty
trying to format and *mount* PC-format 270 disks on my Powermac 8100.
There are a number of drivers out there, all advertising that they mount
everything, but few of them actually do the job. In our facility we have
mostly Mac users, but some PC users, so we must be able to provide both
formats.

3. I have had a Zip 100 Mb unit for about 9 months. It is quiet, fast and
almost trouble free (a pre-formatted cartridge refused to mount and had to
be reformatted; I have not had this problem with any other disks). I
observed roughly similar levels of performance for a Zip 100 on a 486 PC
(parallel port) and the SCSI version on a Powermac 8100; they both rival
hard drive speeds. Using the Iomega driver software on the Mac, the disks
can be either formatted for the Mac, or by using the 'erase disk' menu on
the Mac, a disk can be PC formatted, so this unit essentially solves the
Mac-PC format problems that the Syquest units apparently have more
difficulty with (I'm not an expert here, just giving you my experience).

The Zip unit is nice because it has auto mount and auto eject features.
Put the disk in the unit and it appears on the Mac desktop; drag it to the
trash and it dismounts and ejects. The Syquest units require fooling
around with that eject lever, and novices nearly always seem to want to
eject the cartridge before it is dismounted (and therefore spun down -- a
good way to damage the disk). This is a matter of convenience, but it adds
up over the years in cumulative hassle factor.

There are caveats with the Zip 100 unit. There is no power on/off switch,
just a 12 volt converter plug and line cord, so the unit must be left on
all the time unless you set up a power strip and use that switch to turn
the thing on and off. The unit has only SCSI device numbers 6 and 7
available, which may cause ID conflict problems on some systems, but has
not been a problem with our setup.

Perhaps one of the biggest limitations is for those who plan to use the Zip
100 for backup rather than just external storage. My understanding is that
you can only backup 100 Meg maximum with the Iomega backup software (i.e.
100 Meg maximum as a contiguous save set), so you are out of luck if you
have a larger hard drive (which almost everyone has now). So you would
have to do a backup manually.

4. The Iomega Jaz drive (about $500-600 for the drive, 1 Gig cartridge
about $90) sounds like the best solution for our facility, where users can
amass lots of digital images and analytical data. I have not seen one in
action.

5. I just started seeing ads for another Iomega cartridge drive on tv (not
the Zip, and not the Jaz, and I forget the name). Does anyone know about
this?

From my experience base, I would recommend the Zip 100 for the super cheap
external storage solution -- when it becomes history, we will not have
invested big bucks and can simply copy to a new system and move on. The
Jaz drive sounds good for those with bigger storage needs, and I anticipate
getting one if there are no bad reviews. I was a long time Syquest fan,
but I'm not convinced that they are in for the long haul anymore, and have
been really sluggish to respond to the Iomega market pressure. Syquest has
no competition for the Jaz drive that I know of...

Remember that one of my main priorities is to offer cheap high capacity
media to users of the facility, some of whom come to visit only once and
need to take all their data away with them. More expensive fancy optical
disks etc. are not reasonable to ask these people to purchase, because they
have to go read all this data back at their lab on an equivalent system.
So my decision for external storage automatically commits others to
purchasing the same hardware. A cd writer would be great, but these are
still expensive relative to the cheap Zip solution (for example).

My opinions only, and I have not the slightest financial interest in any of
this, except as a consumer. I'd rather go flying than pay big money for
storage media!

Paul Carpenter


+------------------------------------------------------------+
| Paul K. Carpenter |
| Division Analytical Facility |
| Geological and Planetary Sciences MC 170-25 |
| California Institute of Technology |
| Pasadena, CA 91125 |
| 818-395-6126 (X-ray Lab) 818-568-0935 (FAX, Departmental) |
| paulc-at-arms.gps.caltech.edu |
+------------------------------------------------------------+






From: allardlfjr-at-ornl.gov (Larry Allard)
Date: Mon, 26 Feb 1996 21:26:53 -0500
Subject: Ditto drives

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Paul Carpenter:} } I just started seeing ads for another Iomega cartridge
drive on tv (not the Zip, and not the Jaz, and I forget the name). Does
anyone know about this? { {

Paul:

I'm jealous; you have probably seen the new TV ad blitz by Iomega which
highlights the Ditto Easy 800 tape drives. These drives offer 800 MB for
about $149, and have elegant software which continually backs up your work
even while you are on-line, as I understand. Since Iomega can make plenty
of these, the supply pipeline is easily kept filled, and even though they
are the hottest selling tape backup these days, Iomega has initiated an
advertising campaign to establish their name (sort of like Intel Inside) in
the data storage area, prior to future advertising for Zip and Jaz when
they become more plentiful. I haven't used the Ditto drive as yet, but
plan to purchase one for each of my 3 Macs at home. The Zip travels around
with me at present. At the lab, we have networked tape backup which is
done automatically every night. I think the drives are Colorado units,
which are currently being outsold by the new Dittos. There is also a Ditto
Easy 3200 which offers 3.2MB for about $450, for those with more formidable
backup needs.

BTW, enjoyed your post.

Larry






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Mon, 26 Feb 1996 23:10:42 EST
Subject: ISO Guide 25 Accreditation: Experience

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

Bob Craig wrote:



} The first question that one must ask is why do I need registration,
} certification or accreditation? The answer to this question is
usually
} market driven, at least it was in our situation.

Bob is quite right about everything that he said, however there are
still more reasons why one might want to get accredited.

Let me give some additional reasons:

1] As an independent laboratory, our big fear is professional
liability risk. It is, I am convinced a generally under appreciated
risk, but anyone offering professional opinions and advice, including
microscopy, takes on a certain greater-than-zero risk. Sometimes the
risk can be considerable especially if you have an unhappy client. I
have found in our own analytical laboratories that having the A2LA Iso
Guide 25 accreditation in place is the cornerstone of the very best
loss prevention program imaginable. It sets the standard for quality
and excellence, and there are outside inspectors who come in once every
two years to make sure that all procedures are being followed.

Now this will not guarantee that one won't be sued, but the program
surely does eliminate a good many of the weak spots that exist in the
typical microscopy laboratory operation. Although I might beg, cajole,
encourage, talk nice, talk harsh to certain individuals in our
laboratories, nothing has more impact than having that outside assessor
say the same thing as part of an audit!

2] For those in the laboratory analytical (services) business, from an
insurance and underwriting standpoint, we have found that to whatever
degree we incur costs associated with the accreditation, at least some
of those costs come back to us by way of reductions in the premiums
paid for our professional liability insurance.

3] Furthermore, since the A2LA accreditation and adherence to ISO
Guide 25 is entirely consistent with any good TQM process, to the
degree that the accreditation makes our TQM approach that much more
effective, our cost of rework goes down.


There is a bottom line here that might come as a surprise: At least in
our case, there does not seem to be any "net" cost to become
accredited. Whatever money is spent to become accredited, and to fill
out the extra paperwork, we believe it comes back to us "with
interest" by way of increased business (from clients where quality
really counts), lower insurance costs, and a smaller number of samples
that have to be done over again because they weren't done right the
first time.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================}





From: Rui Costa :      ruicosta-at-esb.ucp.pt
Date: Tue, 27 Feb 1996 10:41:21 +0000 (WET)
Subject: LM-Freeze Drying Technique

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Does any one knows any reference about Freeze Drying Technique, for
preparation of sections for LM ?

Thanks

/-------------------------------------------------------------------------\
| Rui Costa | e-mail: ruicosta-at-esb.ucp.pt |
| Escola Superior de Biotecnologia |telephone: 351-2-5580044 |
| Rua Dr Antonio Bernardino de Almeida |fax: 351-2-590351 |
| 4200 PORTO | |
| PORTUGAL | |
\-------------------------------------------------------------------------/







From: John M. Libert :      jlibert-at-cpcug.org
Date: Tue, 27 Feb 1996 08:17:03 -0500
Subject: Re: Storage Media

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Message-ID: {3133044F.B2C-at-cpcug.org}

David Rothbard wrote:
}
} In the discussion of removable media I have not read anything about
} "storage life" of data. Does anyone know about predicted life for
} data/images stored on Syquest, Zip, CD, or magnetic tape cartridges? Will
} all of these media require re-copying every five years to ensure data
} integrity? That also begs the question as whether of any of these drives
} will around in five years to read them.
}
} David Rothbard
}
} --
} Institute of Paper Science and Technology

Pinnacle Micro claims 30 years for its magneto-optical storage media.

John Libert




From: Owen P. Mills :      opmills-at-mtu.edu
Date: Tue, 27 Feb 1996 08:38:49 -0500
Subject: Re: Mass Storage Media

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Hello,

I want to install a SyQuest drive (currently on a Mac) on a PC. Who, other
than SyQuest, sells an easy to use software driver package for that purpose?
Is any freeware available?

Thanks.


Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: Richard Beanland +44 1327 356363 :      richard.beanland-at-gecm.com
Date: Tue, 27 Feb 1996 13:20:05 +0000 (GMT)
Subject: RE: TEM prep

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Disclose-Recipients: prohibited

Hello Pacqui,
we are a commercial lab doing TEM of semiconductors. We still use
'old' techniques, i.e. we don't use Ron Anderson's tripod polisher and 3M
diamond impregnated plastic polishing films. This is partly due to inertia,
and partly because of cost; we can make up to 10 cross-sections per day per
person just using a few glass slides and two polishing wheels. It would be
nice to upgrade to the tripod technique, but at the moment we can get by with
the cheap and cheerful method (and our charges to external customers are about
1/2 to 1/3 of most other labs). Our main weakness is imaging small devices -
an individual device below about 20um in size is very difficult to hit without
high precision polishing techniques. If you're intending to do individual
devices for failure analysis, then you will do better with the tripod
polisher; otherwise, you may do better using the money to employ someone to
make samples full-time. There are many stages in making a good specimen, and
the best samples come with practice!
We also make diamond TEM grids which are very useful for etching and
ion-milling III-Vs and II-VIs which need good cooling.


Regards,

Richard Beanland
GMMTL Caswell,
Towcester,
Northants
NN12 8EQ
UK
Email richard.beanland-at-gecm.com
Tel +44 1327 356363
Fax +44 1327 356775





From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Tue, 27 Feb 1996 09:23:42 -0500 (EST)
Subject: Re: EM of HCV protease

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From: l_thomas-at-ccmail.pnl.gov
Date: Tue, 27 Feb 1996 09:40 -0800 (PST)
Subject: unsubscribe

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unsubscribe




From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Tue, 27 Feb 1996 09:36:06 -0500 (EST)
Subject: Re: EM of HCV protease

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From: mspilde-at-unm.edu
Date: Tue, 27 Feb 1996 10:02:13 -0700
Subject: WDX

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Does anyone know of a procedure for tuning vertical wavelength dispersive
spectrometers,
specifically JEOL WD spectrometers? These are older, 2-crystal
spectrometers on a 733.

Thanks

Mike Spilde
Dept. of Earth & Planetary Science
University of New Mexico
Albuquerque, NM 87131
Electron Microprobe Laboratory 505/277-5430






From: SNYDER, JOSEPH :      JTS-at-rrccorp.mhs.compuserve.com
Date: 27 Feb 96 17:03:56 EST
Subject: Subscribe

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From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Tue, 27 Feb 1996 16:55:02 -0600
Subject: Re: MULTIPLE USERS ON THE SEM & 840 filaments

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Message-Id: {199602272255.QAA07012-at-mailhub.iastate.edu}
X-Sender: wes-at-pop.ameslab.gov
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My thanks to all who replied to my question about leaving the filament
control knobbed turned up to the saturation point on our JEOL 840A (with W
filament). The consensus appears to be that there is no problem with the
practice.

Note to Bill T.,
We have been leaving our filament current knob turned up over days on
non-use and don't appear to be losing any filament life for it. Therefore,
it appears that the current is turned off internally when the high voltage
is turned off.

----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: huw-at-cemmsa.adelaide.edu.au (Huw Rosser)
Date: Wed, 28 Feb 1996 13:54:30 +1000
Subject: Used EM equipment

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Message-Id: {199602281519.KAA13992-at-thomas.ge.com}
X-Authentication-Warning: thomas.ge.com: daemon owned process doing -bs

G'day
Would anybody in EM land know of the addresses and contact numbers of
dealers in second hand (used) EM's and EM equipment anywhere, not just
Australia.
Reply directly to me if you can.

Huw Rosser
CEMMSA
The University of Adelaide
South Australia





From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 27 Feb 1996 15:29:33 -0500 (EST)
Subject: Re: Low energy vs high energy ion milling

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} ......Multiple Lines Removed.......
...... " " " .......

} Not true!
}
} The sputtering rate/cross-section at the SURFACE of a material
} is not the same as the displacement rate/cross-section in the bulk. The
} simple way to
} think about this is that a surface you only have ~ 1/2 the number of bonds
} that you have in the interior of a bulk. Thus the energy needed to remove a
} surface atom is LESS than that needed to remove one from the bulk. There
} is extensive literature on this unfortunatley, I don't have the references
} readily at hand here at home.
}
Dear Nestor,
In addition to removing the surface atom, do you not also create a
new surface atom from a previously buried atom? If a single atomic layer
were removed, wouldn't this be the equivalent of removing a single layer
from the bulk and rejoining the atoms? I guess if you could remove an atom
from the bulk, but not rearrange the remaining atoms, that would require
more energy. Is that what you mean by "remove one from the bulk"?
Yours,
Bill Tivol




From: em-at-mediacity.com (Ed Monberg)
Date: Tue, 27 Feb 1996 12:02:03 -0700
Subject: Re: media lifetime

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} I agree that magnetic media has a lifetime of 10 years or so, whereas
} optical media is far longer, say 30 years.



Dear Larry, list, et. al,

I remember an article in Scientific American about a year ago
which mentioned responsible estinates that were MUCH MORE PESSIMISTIC:

and on the order of 2 years for tape, 5 years for magnetic disk and
about 10 years for CD.

Todays recording densities make for far more fragile records.
in the "old days" ten years ago, the energy used to store each bit was 10's
of 1000's (!!) times greater than that used today. RAM designers, as a
paralell example, are discussing quantum limits ( 1 electron) of data
cells.

This is NOT a simplistic topic, but one lying under layers and layers of
systems design.

Regards,



Ed Monberg e-mail: {em-at-mediacity.com}

------------------------------------------------------
510-429-1060 Fax 429-1065
LMDC, (Laser Motion Development Co.)
3101 Whipple Road
Union City, CA 94587-1216


For our Most recent Catalogue of "On Hand" EQUIPMENT:
Send empty mail to: {Cat-at-lasermotion.com}

Our web page http://www.lasermotion.com

Is now taking shape!






From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 27 Feb 1996 15:17:57 -0500 (EST)
Subject: Re: MULTIPLE USERS ON THE SEM

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} Now a question,
} We have adopted the practice of leaving the filament current control on our
} JEOL 840A at the proper saturation point (for our normal 15 kv operating
} point) and just turning off the high voltage control for exchanging samples.
} [snip] My question to
} the list is whether there might be any adverse consequences from this
} practice as there do not appear to be any.

Dear Warren,
I assume the filament can be switched off with the current control
in the appropriate position, as is the case for our EM's. If so, there will
be no adverse effect for W, and presumably LaB6. If the filament is still
drawing current with the HV off, there should only be the effect on filament
lifetime equal to the number of cumulative hours of specimen exchange and
the occasional disaster when the filament is not turned off over a weekend.
Yours,
Bill Tivol




From: peggy-at-research.nj.nec.com (Peggy Bisher)
Date: Tue, 27 Feb 1996 14:03:03 -0400
Subject: Calibration of Heating Holder

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We need to calibrate our Gatan heating holder.

Could anyone suggest suitable standard specimens that will help us
calibrate the holder at temperatures up to 800 Celsius?

Many thanks,



****************************************************************************

Margaret(Peggy) Bisher

NEC Research Institute _/ _/ _/_/_/ _/_/_/ _/
4 Independence Way _// _/ _/ _/ _/
Princeton, NJ 08540. _/ / _/ _/_/_/ _/ _/
Tel.: (609) 951-2629 _/ /_/ _/ _/ _/
Fax: (609) 951-2496 _/ _/ _/_/_/ _/_/_/ _/
e-mail: peggy-at-research.nj.nec.com


****************************************************************************

"Home pages are the pet rock of the 90s. We all have them, we all think
they're very cute. But in a few years we're going to look back and be
pretty embarrassed." -- Tony Shepps {toad-at-pond.com}








From: dshubito-at-d.imap.itd.umich.edu (Dennis Shubitowski)
Date: Wed, 28 Feb 1996 13:04:18 -0500
Subject: SEM: Polaroid Film

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Has anyone successfully "unstuck" Polaroid Type 55 negatives
that have stuck together while drying? A water soak did not
seem to help nor did Photoflo.

Thanks,

Dennis Shubitowski
University of Michigan
School of Dentistry






From: philf-at-NEWTON.UMSL.EDU (Philip Fraundorf)
Date: Wed, 28 Feb 1996 12:19:13 -0600
Subject: CTEM's "Line of no contrast", and Coulomb's Law

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X-Sender: c4647-at-slvaxa.umsl.edu (Unverified)
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shsiriw-at-slvaxa.umsl.edu, s924939-at-UMSLVMA.UMSL.EDU,
s923023-at-UMSLVMA.UMSL.EDU, khbrewer-at-ucs.indiana.edu, FBaugh-at-aol.com,
swpgarv-at-slvaxa.umsl.edu, cs106-at-viper.wg.waii.com,
agray-at-ccsmtp.memc.com, dawkins-at-kepler.umsl.edu,
s922712-at-slvaxa.umsl.edu, s987041-at-slvaxa.umsl.edu, pnellesen-at-aol.com
X-Mailer: {PC Eudora Version 1.4}

I just stumbled upon a graphical connection between
electrostatic field components from Coulomb's law and the
"line of no contrast" one finds in dark-field images of
small strained crystal defects in TEM darkfield imaging.
The connection is obvious "on reflection", but the experience
of seeing what looks like a TEM image flowing from simple
electrostatics calculations might be worth a visit, at
least for folks who apply "physics filters" to TEM images, to
my E&M course's triplet-visualization link at
{http://newton.umsl.edu/~philf/triplet.html} .

Enjoy. /philf :)

//\/\/\/\---}
// Phil Fraundorf Physics&Astronomy/CME 314-5165044 philf-at-newton.umsl.edu
\\ B503 U.Missouri-SL St.Louis MO 63121 USA http://newton.umsl.edu/~philf
\\/\/\/\/\/\/\/---}





From: bsgphy3-at-uconnvm.uconn.edu (JIM ROMANOW)
Date: Wed, 28 Feb 1996 15:02:47 -0500
Subject: Thank You

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Thank you to everyone who responded to my request for critical point drying
references and information.

Jim Romanow
Electron Microscopy Facility
Physiology and Neurobiogy Department
The University Of Connecticut
Storrs
bsgphy3-at-uconnvm.uconn.edu






From: Sally Shrom :      sally-at-retina.anatomy.upenn.edu
Date: Wed, 28 Feb 1996 15:49:24 -0500 (EST)
Subject: LM and EM tech schools

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Posted-Date: Wed, 28 Feb 1996 15:58:08 -0500

Are there any 2 year tech schools in the Philadelphia area?
Sally Shrom




From: Dong Lee :      dong.lee-at-materials.oxford.ac.uk
Date: Wed, 28 Feb 1996 21:02:58 +0000 (GMT)
Subject: LM and EM tech schools

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From: evansnd-at-ornl.gov (Neal D. Evans)
Date: Wed, 28 Feb 1996 08:57:43 -0500 (EST)
Subject: Calibration of Heating Holder

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Peggy,

There is a straightforward way of calibrating local specimen temperatue when
using a heating holder:

D. C. Paine, D. J. Howard, and N. D. Evans, "In Situ TEM Studies of the
Effect of Misfit Strain on the Kinetics of Si1-xGex Solid Phase Epitaxy:
Temperature Calibration & Surface Effects," Electron Microscopy 1992: Proc.
50th Annual Meeting of the Electron Microscopy Society of America, G. W.
Bailey, J. Bentley, and J. A. Small, eds., San Francisco Press, (1992), 1344-45.

To summarize, the thermocouple provides an accurate measure of the furnace
temperature. However, actual specimen temperature, as a function of furnace
temperature, will vary (at best) slightly from specimen to specimen. This
is due to differences from specimen to specimen in the conduction path, that
is, differences in contact resistance at the furnace/specimen interface as
well as specimen geometry affects.

Specimens having cracks or other features which would restrict or alter heat
flow will have greater uncertainty associated with actual temperatures.

Regards,

Neal





From: Krueger, Eugene :      krueger.eugene-at-mayo.edu
Date: Wed, 28 Feb 1996 16:01:01 -0600
Subject: Locating injected cells on TEM

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Hello All

Help!

I have been injecting cultured hepatocytes with antibodies and I am seeing
interesting morphological changes on the LM. I now need to prepare injected
cells for the TEM (not a problem). I am wondering what electron dense
material(s) could be included in the injectate to show that a particular cell
was injected (I want non-injected cells included on the coverslip as
controls). The material must not interfere with good specimen appearance,
and it also must be injectable (not toxic or clog my needles). Any help will
be greatly appreciated!!!



Thanks in advance!
Eugene Krueger
Mayo Foundation
GI Research
krueger.eugene-at-mayo.edu




From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Wed, 28 Feb 1996 12:16:48 -0800
Subject: TEM;phosphate ppt in seawater

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Howdy all

a user brought me a sample of gelatinous unicellular algae she
preserved in 2% phosphate buffered glutaraldehyde. Unfortunately, it was a
marine sample, so when she fixed it, the salts in the seawater
precipitated. She can't repeat the experiment. Any way to salvage her
sample--i.e., separate the cells from the ppt, or get the salts back into
soln without detroying the cells? We appreciate that the precipitation may
have ruined her cell preservation as well, but we won't know for sure till
we can embed and section.

thanks in advance

steve

Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego, CA 92182-4614
phone: (619)594-4523
fax:(619)594-5676
email:sbarlow-at-sunstroke.sdsu.edu






From: hiroyuki-at-alex.ucsd.edu (Hiroyuki Hakozaki)
Date: Wed, 28 Feb 1996 14:44:00 -0800
Subject: How to unsbscribe?

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Message-Id: {9602282237.AA08163-at-alex.UCSD.EDU}

Hi,
Sorry for sending unsbscribe message for this list.
But I couldn't unsubscribe by sending unsbscribe message to
listserv-at-sparc5.microscopy.com.
Could anybody tell me how can I unsubscribe? I also tried to get help from
listserver by sending help command. But no response.

I'm really sorry for sending unsbscribe message to entire list.

Hiro.
===========================================================
Hiroyuki Hakozaki
Laboratory for Neurocytology Department of Neurosciences
School of Medicine University of Calofornia , San Diego
La Jolla , CA 92093-0608
Tel (619)-534-4583 or 2583
Fax (619)-534-7497
E-mail hiroyuki-at-ncmir.ucsd.edu
===========================================================





From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Thu, 29 Feb 1996 14:47:49 +1100
Subject: SEM: Microwave fixation of articular cartridge

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Has anyone on the List had any experience with microwave fixing /processing
of articular cartilage for scanning electron microscopy?

We have a user wishing to do this and so we can't find any references to
this technique.

If anyone can help with ideas or protocols we would like to hear from you.

Thank you very much.

Please send replies to: richard.lander-at-stonebow.otago.ac.nz

Richard Lander


Richard Easingwood
South Campus Electron Microscope Unit
School of Medical Sciences
University of Otago
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD















From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 28 Feb 1996 15:06:19 -0500 (EST)
Subject: Re: EDX sum peak

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} Hi Mark;
}
} [snip] but are you sure you don't have
} aluminum present? I would do a couple of things to check this;
}
} 1) Collect a "blank" spectrum of the matrix and see if you get a peak at
} 1.49 keV. If you do, you either have some aluminum in the matrix,

To add a bit to Bob's reply, Br has an L-peak overlapping the Al
K-peak. Although it is likely that a peak at ~1.49 keV is Al, check the
10-20 keV range for other peaks at the Br K alpha & beta positions. I
have seen sediments with Al and no Br in one grain and Br in a nearby grain.
Yours,
Bill Tivol




From: erik-at-uclink.berkeley.edu (Erik Pauls)
Date: Wed, 28 Feb 1996 20:59:54 -0800
Subject: Software for Display of Stereo Pairs

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If you are using a Mac, there are several different resources as part of
NIH's Image freeware. I believe the best access is at
http://rsb.info.nih.gov/nih-image/ This is a very good program for image
manipulation for Macsters (that's why I'm posting to the list). A PC
version is in the works too.

I know that I've seen stereo pairs, of the red/green variety, discused in
the recent past. If there is nothing directly listed at NIH's web page
there is an Image list that should have archives. The listproc address is
LISTPROC-at-SOILS.UMN.EDU, and the list is NIH-IMAGE. (Cap.s not necessary.)

Erik
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Cretaceous Liability: Tyrannosaurus Wrecks

Erik Pauls
erik-at-uclink.berkeley.edu





From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Wed, 28 Feb 1996 22:59:30 EST
Subject: LM and EM tech schools

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On Feb. 28, Sally Shrom wrote:


--Subject: LM and EM tech schools
}
} Are there any 2 year tech schools in the Philadelphia area? Sally
Shrom

I would suggest you contact the following:

West Chester University
West Chester, PA 19380

Attn: Prof. Arthur Smith
Dept. of Geology

e-mail: asmith2-at-wcupa.edu

They have an outstanding program, Prof. Smith has been doing this for
some years and his students have no difficulty finding jobs, even in
today's difficult job climate.

Hope this helps. BTW, no connection here with West Chester University!

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================

-------- REPLY, End of original message --------







From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Wed, 28 Feb 1996 17:17:57 -0600
Subject: Re: SEM: Polaroid Film

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In message {v02120d01ad5a491be151-at-[141.211.157.94]} Dennis Shubitowski writes:
} Has anyone successfully "unstuck" Polaroid Type 55 negatives
} that have stuck together while drying? A water soak did not
} seem to help nor did Photoflo.
}
} Thanks,
}
} Dennis Shubitowski
} University of Michigan
} School of Dentistry
}
}

Dennis, Yes, I have successfully unstuck pairs of Polaroid negs. Actually, the
water soak will work, but you have to soak them for a long time, at least
overnight, say 12-16 hours. Then you have to slowwwwwwwly pull them apart to
avoid pulling the emulsion off. A "pucker" mark may remain where they were
joined, but usually its translucent so that a positive print will seldom reveal
the pucker mark, especially on "busy" image detail, and as long as original
image density was not disturbed.

When this happens to my negs, its usually in a spot no larger than the size of a
quarter (25 cent piece). If its most of the area of the negs, that is serious
trouble.

When I dry Polaroid negs in racks, I skip slots so that there is lots of space
between adjacent negs to prevent sticking from occuring as the negs "wiggle" as
they air dry.


--

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: Steven D. Majewski :      sdm7g-at-Virginia.EDU
Date: Wed, 28 Feb 1996 12:31:59 -0500 (EST)
Subject: Re: media lifetime

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On Tue, 27 Feb 1996, Ed Monberg wrote:

} } I agree that magnetic media has a lifetime of 10 years or so, whereas
} } optical media is far longer, say 30 years.
}
} Dear Larry, list, et. al,
}
} I remember an article in Scientific American about a year ago
} which mentioned responsible estinates that were MUCH MORE PESSIMISTIC:
}
} and on the order of 2 years for tape, 5 years for magnetic disk and
} about 10 years for CD.
}

Also, you should read the 'fine print' when it comes to statements
about media lifetime estimates. DAT DDS tapes, for example, have
a 10 year estimated lifetime. What that actually means is that if
you use a tape once to record and put it on a shelf in a temp. and
humidity controlled environment, you are likely to be able to
read it back in 10 years. Divide that by some factor if stored
under less than ideal conditions. I was interested in archival
use, rather than backups, where I may need to read back the data
many times over that decade. Ask about how many passes of tape
over the heads, including rewinding and forwarding, the tape is
rated for, and you get a figure that may translate into only a
few years of use (depending).

Data format is another problem. I think the ISO CD-ROM standard is
in wide enough use to rely upon, but for magneo-optical disks,
my experience has been that changing SCSI controller, operating
system or other software, even ( with our Pinnacle MO drives )
chaning from one version of DOS to another, is enough to make
the data effectively unreadable. ( The documentation necessary
to write a program to retrieve that data is available, but not
from Pinnacle -- we had to talk to their suppliers to get any
useful information. ) I was also told by Pinnacle that they
had changed suppliers for their SCSI controller cards, and if
we bought a newer model, they would not guarantee that we would
be able to read old data on the new drive. I don't know that
Pinnacle is any worse that any other supplier -- that's just
who I had my unhappy experience with. However, none of the
salespeople will bring up the subject, and few will even understand
your concerns, if you try to ask them how likely you are to be
able to read your data 10 years from now.


ISO 9660 CD format and 'tar' files on DDS tapes (in that order)
are the only formats in which I have much long term (decade) faith.


---| Steven D. Majewski (804-982-0831) {sdm7g-at-Virginia.EDU} |---
---| Computer Systems Engineer University of Virginia |---
---| Department of Molecular Physiology and Biological Physics |---
---| Box 449 Health Science Center Charlottesville,VA 22908 |---
[ "The grass is always greener, except at t=0" - Stan Kelly-Bootle ]





From: margo-at-jaguar.dote.hu (Margit Balazs)
Date: Wed, 28 Feb 1996 11:01:11 +0100 (MET)
Subject: Re: media lifetime

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unsubscribe





From: Mount, Richard :      MOUNTR-at-hermes.is.sickkids.on.ca
Date: Thu, 29 Feb 1996 08:50:00 -0500
Subject: Re: SEM Polaroid films

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Message-Id: {Megw.230326-at-hermes}


{Text_1}
Dennis Shubitowski asked "Has anyone successfully "unstuck"
Polaroid Type 55 negatives that have stuck together while
drying?"

I have had limited success with an overnight Photoflo soak
followed by ultrasonication. Films stuck emulsion to film
back seem to separate easier than those stuck emulsion to
emulsion.

------------------------------------------------------------
Richard J. Mount Phone: 416-813-6551
Auditory Science Laboratory
Department of Otolaryngology FAX: 416-813-5036
The Hospital for Sick Children
555 University Ave.
Toronto, ON, Canada M5G 1X8

e-mail:richard.mount-at-mailhub.sickkids.on.ca




From: Joe D Geller :      geller-at-world.std.com
Date: Thu, 29 Feb 1996 09:42:27 -0500 (EST)
Subject: Science Fair Projects- Grade 8

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My family would be gratefull for suggestions involving the SEM. We are
thinking about putting iron and stainless steel strips into various pH
solutions to measure material loss and corrosion. SEM photos of the etch
pits should make good illustrations.

Do you metallurgical/material science types have any suggestions to
help? pH choices, time of exposure, how to introduce oxygen to enhance
corrosion, etc.

Thanks,
Joe Geller
Geller MicroAnalytical Lab
426e Boston St.
Topsfield, Ma 01983-1216
508 887-7000 fax 887-6671




From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Thu, 29 Feb 1996 11:33:50 -0500 (EST)
Subject: Re: Used EM equipment

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On the subject of used EM equipment, we have an old Phillips TEM that we
were considering putting up for sale. We were unsure whether there would
be any demand for these instruments. (It needs a new condenser lens).
I'm not sure of the model number, but I can get specifics if anyone is
interested.

Cheri Owen
Detroit Neurotrauma Institute
Wayne State University
Detroit, MI
313-577-4648




From: randy nessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Thu, 29 Feb 1996 09:01:09 -0600 (CST)
Subject: Origin of carbon? film?

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Hi All,
I have a sample that I need some help with. We have received some
samples that are from private homes. The residents are reporting a film (soot)
forming on the wall above heat registers, on windows, and settling on furniture.
These homes have newly installed high-effiency furnaces, and the question is
whether the deposits are from the furnace, or some other source. We have
analyzed some of the soot, and found it to be carbon with EDS. Now comes the
tough part, is it possible to determine the source of this carbon (whether it
comes from the burning of natural gas, a cigarette, or a candle). Any help in
sampling and analyzing techniques would be greatly appreciated.
Thanks,

Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu





From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Thu, 29 Feb 1996 10:58:41 EST
Subject: Re: Software for Display of Stereo Pairs

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I have successfully done this on a PC and Mac using Adobe Photoshop and 2
appropriately parallaxed images. Both must be changed to RGB color, the
left (or zero degree) image saturated to red, and the other image
saturated to either green or blue. Copy one image to the clipboard and
then paste it to the other. Set the composite controls to 50% opacity
with preview on. Viewing the image through red/green glasses, you can
then properly register the pair.

It's a fairly crude technique, but it does work.

-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Owen P. Mills :      opmills-at-mtu.edu
Date: Thu, 29 Feb 1996 15:32:10 -0500
Subject: Replacement Bulbs for Vickers Microscopes

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Hello,

A colleague asked if I would submit this to the listserver.

snip..
} I am looking for replacement bulbs for 20-year old Vickers reflected light
} petrographic microscopes. The bulbs we have been using are encoded
} Phillips-Holland 13702C 6 volt 15 watt. Any help in locating such bulbs
} will be greatly appreciated.
snip..

Thanks in advance.

Owen
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: Owen P. Mills :      opmills-at-mtu.edu
Date: Thu, 29 Feb 1996 15:32:10 -0500
Subject: Replacement Bulbs for Vickers Microscopes

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Hello,

A colleague asked if I would submit this to the listserver.

snip..
} I am looking for replacement bulbs for 20-year old Vickers reflected light
} petrographic microscopes. The bulbs we have been using are encoded
} Phillips-Holland 13702C 6 volt 15 watt. Any help in locating such bulbs
} will be greatly appreciated.
snip..

Thanks in advance.

Owen
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: lag-at-pegasus.cc.ucf.edu (Lucille A. Giannuzzi)
Date: Thu, 29 Feb 1996 09:42:34 -0500
Subject: grad student needed

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Message-Id: {v01530502ad5b6ba2e6b4-at-[132.170.199.100]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Graduate Student is Needed!!!

University of Central Florida
Dept. of Mech. and Aero. Eng.
Materials Science and Engineering Program

A M.S. or Ph.D. level graduate student is needed to perform research in the
area of thin films with applications in electronic materials in
collaboration with Lucent Technology (formerly AT&T Microelectronics).
Research will be performed on Campus at the University of Central Florida,
Orlando, and at the research facilities of Lucent also in Orlando.
Research may begin in the Summer 1996 semester. Please address
inquiries/resumes to:


*************************************************************************
Lucille A. Giannuzzi, Ph.D.
Assistant Professor

Materials Science and Eng. Program
Dept. of Mechanical and Aerospace Eng. phone (407) 823-5770
University of Central Florida fax (407) 823-0208
4000 Central Florida Blvd. email lag-at-pegasus.cc.ucf.edu
Orlando, FL 32816-2450 USA
*************************************************************************







From: Yi Huang :      yi_huang-at-QMGATE.ANL.GOV
Date: 29 Feb 1996 15:59:29 -0600
Subject: Mo aperture grids

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Message-ID: {n1386512564.39588-at-qmgate.anl.gov}

Subject: Time:3:40 PM
OFFICE MEMO Mo aperture grids Date:02/29/96

Hi,
I am looking for TEM grids with single hole of 0.8, 1.0 and 1.5 mm in diameter
and slot of 1x2 and 0.5x2 mm. I tried several vender and did not succeed.
Anybody know a source of these grids?
Thank you in advance for your help.

Yi Huang
Argonne National Lab/MSD 212
Argonne, IL 60439
tel: 708-252-5181
fax: 708-252-4798
yi_huang-at-qmgate.anl.gov





From: Yi Huang :      yi_huang-at-QMGATE.ANL.GOV
Date: 29 Feb 1996 16:00:27 -0600
Subject: Mo aperture grids

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Message-ID: {n1386512563.39536-at-qmgate.anl.gov}

Subject: Time:3:40 PM
OFFICE MEMO Mo aperture grids Date:02/29/96

Hi,
I am looking for Mo TEM grids with single hole of 0.8, 1.0 and 1.5 mm in
diameter and slot of 1x2 and 0.5x2 mm. I tried several vender and did not
succeed. Anybody know a source of these grids?
Thank you in advance for your help.

Yi Huang
Argonne National Lab/MSD 212
Argonne, IL 60439
tel: 708-252-5181
fax: 708-252-4798
yi_huang-at-qmgate.anl.gov





From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Thu, 29 Feb 1996 09:08:20 -0600
Subject: Re: Partitioning Hard Drives

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Message-Id: {199602291508.JAA20746-at-mailhub.iastate.edu}
X-Sender: wes-at-pop.ameslab.gov
X-Mailer: Windows Eudora Pro Version 2.1.2
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Partitioning becomes a balancing act. There are two rules to remember that
govern partitioning.

Rule 1 - You are allowed a maximum of 64K clusters per partition and each
cluster has to be a power of 2 bytes long, hence 2048, 4096, 8192, etc.

Rule 2 - About half a cluster (on the average) is wasted per file.

Now the balancing act - If you will be storing lots of small files then
small partitions are good in order to not waste too much space. But you want
large enough partitions to do you some good. Of course, most EM image data
files are quite large so that the number of clusters per file will be larger
and the fractional wasted space less. You will want a large partition just
to get a reasonable number of files on it.

There is also the hassle of trying to keep track of all the partitions.
System and application files can reside on one partition, smaller data files
on another, large image files on a third. But how do you use the remaining
partitions? Perhaps they can be used to assign a partition to each user.
However, I still have a PDP-11 system with a 170 MB drive (it was big back
then) which *had* to be partitioned into segments no larger than 32 MB. We
distributed users around as space was availabe. But it was hard to remember
where we put them without a good search utility (which we used).

Practically, we have three 850 MB drives in our group that I have
partitioned into 4 pieces each. We defined 1 127 MB segment for smaller data
files, and split the remainder up into 3 partitions of about 240 MB each;
one is used for system files, one for large images, and the third is used
for temporary projects but is mostly empty.

Hope this helps. BTW, Partition Magic caught my eye the other day. It would
be nice to adjust such things on the fly.

At 11:06 AM 2/28/96 -0800, you wrote:
}
} I have been following the CD thread etc. I thought someone might be
} interested in this: Bought a notebook with 800MB HD and the usual installed
} software taking up about 60 MB. Using Partition Magic 2.0, I was able to
} repartition the HD on-the-fly and created 500MB and 250MB partitions (42 MB
} reserved for suspend mode). In doing so, I recovered an extra 10 MB due to
} the smaller sector sizes used. That's a nice feature of PM in that it can
} change sector sizes on- the-fly. What I mean by "on-the-fly" is that you
} don't have to backup the HD, re-partition it, and then reinstall the
} software; you just run the software, create whatever partitions and sizes
} you want, and you are done. It can do a lot more than that but you can get
} information from the vendor.
}
} Since the sector size changes with partition size (the break points are at
} 128, 256, and 512 or some such numbers), has anyone partitioned a large HD
} into 128 MB drives for the greatest storage efficiency or are there down
} sides to that? Would be interested in hearing your thoughts on that.
}
} Disclaimer: I have no financial interest in PowerQuest (mfg. of Partition
} Magic).
}
} Damian Neuberger
} neuberd-at-baxter.com
}
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 29 Feb 96 18:53:20 EST
Subject: Re: Mo aperture grids

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Yi-

You may want to try SPI Supplies. They have a nice section on their TEM grids
which can be found on their WWW Site. You can reach it as follows:

WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html

Good Luck!

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
sbt-at-msa.microscopy.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: emccjb-at-ttuhsc.edu (Charles J. Butterick)
Date: Thu, 29 Feb 1996 11:48:03 -0600
Subject: Re: Locating injected cells on TEM

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I worked with an electrophysiologist who used procion brown. Didn't seem
to harm the ultrastructure but did make the cells more electron dense than
the surrounding retinal neurons. Ken-iche Naka would stick and record
retinal neurons with a dye loaded micropipette. Once the recording was
done, then he injected the dye into that cell. Recovering the cells was a
bit arduous.


} Hello All
}
} Help!
}
} I have been injecting cultured hepatocytes with antibodies and I am seeing
} interesting morphological changes on the LM. I now need to prepare injected
} cells for the TEM (not a problem). I am wondering what electron dense
} material(s) could be included in the injectate to show that a particular cell
} was injected (I want non-injected cells included on the coverslip as
} controls). The material must not interfere with good specimen appearance,
} and it also must be injectable (not toxic or clog my needles). Any help will
} be greatly appreciated!!!
}
}
}
} Thanks in advance!
} Eugene Krueger
} Mayo Foundation
} GI Research
} krueger.eugene-at-mayo.edu



Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Thu, 29 Feb 1996 12:19:36 -0600
Subject: Re: SEM Polaroid films

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In message {Megw.230326-at-hermes} "Mount, Richard" writes:
}
} {Text_1}
} Dennis Shubitowski asked "Has anyone successfully "unstuck"
} Polaroid Type 55 negatives that have stuck together while
} drying?"

{text 2}

} Films stuck emulsion to film back seem to separate easier than those
} stuck emulsion to emulsion.
}
} ------------------------------------------------------------
} Richard J. Mount Phone: 416-813-6551
} Auditory Science Laboratory
} Department of Otolaryngology FAX: 416-813-5036
} The Hospital for Sick Children
} 555 University Ave.
} Toronto, ON, Canada M5G 1X8
}
} e-mail:richard.mount-at-mailhub.sickkids.on.ca


I agree with Richard, above, that film back stuck to emulsion is easier to
seperate, and this is the case I usually run into as I dry my Polaroid negs in
racks with the emulsion of one facing the back of the next neg.

A point that I left out in my previous note describing successful use of
overnight water soak to seperate stuck negs is how I set this up. As with many
techniques, success lies in the details of the method. I load a pair of stuck
negs into my usual neg drying rack with edges of one neg in one set of slots,
edges of other neg in adjacent set of slots. The effect is to separate the negs
where they are not stuck to enable water to get directly to the stuck area.
Success depends on getting good water soak and softening of the film emulsion.
If you were to simple lay the two stuck negs flat in a pan of water, might not
get as good a soak as when the films are seperated a bit to allow water access.

I hope this helps.




--

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 29 Feb 1996 14:59:06 -0500 (EST)
Subject: Re: TEM;phosphate ppt in seawater

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} a user brought me a sample of gelatinous unicellular algae she
} preserved in 2% phosphate buffered glutaraldehyde. Unfortunately, it was a
} marine sample, so when she fixed it, the salts in the seawater
} precipitated. She can't repeat the experiment. Any way to salvage her
} sample--i.e., separate the cells from the ppt, or get the salts back into
} soln without detroying the cells? We appreciate that the precipitation may
} have ruined her cell preservation as well, but we won't know for sure till
} we can embed and section.
}
Dear Steve,
I am not an expert in this, but FWIW, I would try rinsing in buf-
ferred saline using a lowish concentration of a buffer such as tris or
acetate. This might wash off or redissolve the external ppts. I wouldn't
think anything would get ppt out of the interiors of the cells but still
preserve the morphology. I'd pay attention to the osmolarity of the rinse,
although the 2% PO4+glut probably swelled the cells if they came directly
from seawater. Good luck.
Yours,
Bill Tivol




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Thu, 29 Feb 1996 21:01:47 EST
Subject: Mo aperture grids

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On Feb. 29, the following question was asked:

} Subject: Mo aperture grids
}
} I am looking for TEM grids with single hole of 0.8, 1.0 and 1.5 mm in
} diameter and slot of 1x2 and 0.5x2 mm. I tried several vender and did
not
} succeed. Anybody know a source of these grids?
}
We have had in production Mo support "rings" for use during the ion
milling of samples for quite some time. They are 3mm diameter OD with
an ID of 1.5 mm. Thickness is 0.5 mm. These should work out just fine
for you.

You can find them either on page 74 of the current SPI Supplies
SourceBook or else in our electronic on-line catalog at the ULR given
below. They will be found on the "page" with the ultrasonic disc
cutter.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 29 Feb 96 18:47:20 EST
Subject: NEW Tripod Polisher Workshop

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REDUCED FEE REGISTRATION DEADLINE APRIL 30, 1996

Workshop on Tripod Polishing

Workshop Objective
This course will cover all aspects of pre-thinning and focus on final thinning
via Tripod Polishing. Due to the limited class size and the extensive hands-on
opportuinities, this course is well suited to novices as well as advanced
Tripodders. The course will include sections on:

How to do it and why should I?
What's really going on and what am I really seeing?
How to prepare small, specific area cross-sections.
The problem of wildly differing materials (eg tungsten).
Rapid preparation of TEM cross-sections.
Preparation of a wide range of materials: semiconductors, ceramics, metals,...

Hands-on Opportunity
This course will be unique in that it will provide a hands-on opportunity for
every class participant. Tripod Polishers, Polishing Wheels, and pre-thinning
equipment will be made available to all participants and actual samples will be
prepared - by the students - as part of the course. This is a great opportunity
to get your hands dirty and actually learn by doing. The instructors will walk
you through each step of the process and then let you loose on the equipment.
This course is designed to teach the Tripod Polishing technique. Silicon
samples will be provided to the students and used as the basis for the course
teaching.

Workshop Location and Dates
South Bay Technology - San Clemente, CA
Dates: Friday & Saturday - June 14-15, 1996
Friday & Saturday - October 18-19, 1996

Previous Participants (partial list)
INTEL, AMD, Motorola, LSI Logic, Conner Peripherals, Univ of Maryland, Univ of
New Mexico, UNAM (Mexico), LG Electronics (Korea), Battelle, MEMC, MVA Inc.,
Univ of Michigan, U.S. Bureau of Mines, IBM, Naval Research Lab, Purdue Univ,
Univ of Alabama, Univ of Arizona, Univ of Colorado, Univ of Wisconsin.

Class Size
Due to the intensive hands-on aspects of this course, class size will be
strictly limited to 10 participants.

Registration Fee: $795 (includes lunches and Friday night Dinner)
$695 if registration fee paid by April 30, 1996 for June
Workshop and by August 31, 1996 for October Workshop

Registration Deadline: 30 days prior to workshop

For additional Information: Monica Pflaster
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673
TEL: 800-728-2233
FAX: 714-492-1499
e-mail: sbt-at-msa.microscopy.com




Registration Form

To register for the workshop, please fill out this form and send it, with
registration fee to:

South Bay Technology, Inc.
Workshop on Tripod Polishing
1120 Via Callejon
San Clemente, CA 92673

Payment must be made in the form of a check, money order, Visa or MasterCard.
Checks must be drawn on a U.S. Bank and made payable to South Bay Technology,
Inc. Credit card orders by FAX may be sent to South Bay Technology at
714-492-1499.

Name:


Affiliation:


Address:




City: State:
Zip: Country:_________
Telephone: FAX:

e-mail:________________________

Primary sample type:




VISA MasterCard Card #_________________________________

Expiration Date________ Signature of Cardholder_________________________

Cardholder name (Please print):________________________________________

Please circle workshop you are registering for: June 14-15 October
18-19





From: Wei.Liu-Ying-at-mb.luth.se
Date: Fri, 1 Mar 1996 14:31:14 +0800
Subject: Software for SAED indexing

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Dear microscopists,

I am looking for software (Macintosh) for SAED indexing. Does anyone know a
source of such software? Thanks for your help.
Yours sincerely,
Liuying Wei






From: peling-at-amnh.org (Peling Melville - Interdepartmental Facilities)
Date: Fri, 1 Mar 1996 11:04:26 -0500
Subject: Etching Iron Oxide?

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Message-Id: {199603011445.IAA02762-at-Sparc5.Microscopy.Com}

A coworker of mine wanted to know if there was anything to etch fossils
that have been altered to iron oxide.

Thanks in advance,
Peling Melville

--------------------------------------------------------------
Peling Fong Melville
Senior Scientific Assistant
Interdepartmental Facilities
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 U.S.A.
******************************
E-mail: peling-at-amnh.org
Work #: (212) 769-5469
FAX #: (212) 769-5495






From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Fri, 1 Mar 1996 11:24:26 -0700
Subject: Ultramicrotome needed by non-profit foundation

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A private non-profit foundation [401(3)(C) status] is looking for a used
ultramicrotome and glass knifemaker through a donation or inexpensive
purchase.

If you can help, please contact Dr. Gary Sibert at (505) 434-1725.

Please don't reply to this list. Thanks,

John
chandler-at-lamar.ColoState.EDU






From: lifan chen :      lfchen-at-unm.edu
Date: Fri, 1 Mar 1996 11:19:16 -0700 (MST)
Subject: Hello!

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Dear Sir/Madam:

I am a microscopist in TEM Laboratory of the University of New Mexico.
I hope to get some new information from you every day. Could you send me
some infromation every day if possible?

Thank you very much!!!





From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Fri, 1 Mar 1996 12:52:10 -0700
Subject: Philips TEM available

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TEM available:

Philips CM10
Purchased 1987
With EDAX EDS system and ancillary equipment for materials lab
Currently under service contract with Philips

Contact: Lori or Frank Visconti
(303) 238-5574 (until March 21)
(303) 987-9178

Do not reply to this list. Thanks,

John
chandler-at-lamar.ColoState.EDU






From: T. Page Owen Jr :      tpowe-at-conncoll.edu
Date: Fri, 1 Mar 1996 15:24:10 -0500 (EST)
Subject: Azide and plant cell ultrastructure

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Message-Id: {199603012106.PAA03222-at-Sparc5.Microscopy.Com}


Does anyone know if adding sodium azide to a plant cell suspension
culture (final concentration 0.02%) affect its ultrastructure?

It was added 30 minutes before fixing the cells in 2.5% glutaraldehyde.
Azide was used in order to stop cell activity at a specific time point.
The main interest in the ultrastructure analysis is to look at the Golgi
apparatus, cell wall, plasma membrane, and endoplasmic reticulum.

If it does (or doesn't) affect the ultrastructure; How? Which
organelles are affected the most? Are there references in the literature
about it?

Thanks in advance for your input.

Manrique Rojas
Page Owen
Department of Botany
Connecticut College





From: TKWZ51A-at-PRODIGY.COM (MR ALEXANDER W GINGELL)
Date: Fri, 01 Mar 1996 18:15:35 EST
Subject: Azide and plant cell ultrastructure

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-- [ From: Alex W. Gingell * EMC.Ver #2.10P ] --

Dear Microscopists,

I am sending this on behalf of a customer who does not have e-mail
access.

He would like to communicate with anyone who has a Tracor TN5500
analyser for sale. The customer is in Southern Ontario (Canada), so
would only consider units in Canadian or USA due to shipping costs.

Please e-mail to me directly, and I will pass the information on to my
customer.

Thanks in advance for your assistance.

Alex





From: Alan S. Pooley :      pooley-at-ahab.rutgers.edu
Date: Fri, 1 Mar 1996 10:46:11 -0500 (EST)
Subject: unsticking polaroid negatives

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(snip below)

YES, virginia, polaroid negatives can usually be unstuck, just soak in
very hot water about 10 -20 minutes, then GENTLY pull them apart, GRADUALLY
turning and pulling a little untilthe whole gelatine that is stuck gets
soaked through and losened. Some times if more completely dried out, the
emulsion of one will be stuck to the second, usually the second can be saved
completely, but some of the first will be lost (natually the center, best
part of the image)
good luck

} Unsticking polaroid negatives
} that have stuck together while drying? A water soak did not
} seem to help nor did Photoflo.

} Thanks,

} Dennis Shubitowski
} University of Michigan
} School of Dentistry


Alan S. Pooley ,PhD Bivalve shell SEM & shape analysis
SEM/Morphometrics lab
Marine & Coastal Sciences
Rutgers University
908 932 8959 ext 225
Pooley-at-ahab.rutgers.edu





From: Elinor Solit :      cambrex-at-world.std.com
Date: Fri, 1 Mar 1996 11:56:32 -0500 (EST)
Subject: Re: How to prepare beryllium metal?

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Hi Yolande,

Suggest you try Paul Fischione. He is at 412-325-5444.

Best regards,

Ellie Solit

On Thu, 29 Feb 1996, Berta, Yolande wrote:

}
} Dear Microscopists,
}
} We have a chunk of beryllium, and we would like to know the thickness of the
} oxide layer that forms on the metal within a given time interval. SEM hasn't
} shown the oxide layer, so now we are thinking that maybe a TEM cross-section
} specimen would be the thing to try.
}
} Has anyone prepared beryllium for TEM before? How? Ion milling?
} Jet-polishing? How was it cut to keep the dust at a minimum?
}
} Yolande Berta
} School of Materials Science and Engineering
} 778 Atlantic Dr.
} Atlanta, GA 30332-0245
} (404)894-2545
} yolande.berta-at-mse.gatech.edu
}




From: Joe D Geller :      geller-at-world.std.com
Date: Fri, 1 Mar 1996 09:56:12 -0500 (EST)
Subject: Re: How to prepare beryllium metal?

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This seems to be a perfect application for Auger electron spectroscopy
which is sensitive to the first few monolayers on the surface. The
surface is ion beam sputtered to determine the oxide depth. Auger
spectroscopy is very sensitive to Be.

If you don't have your own instrument, or one on campu, there are several
commercial laboratories providing this service, including ours.

Joe Geller
Geller MicroAnalytical Lab
426e Boston St.
Topsfield, MA 01983-1216
508 887-7000

On Thu, 29 Feb 1996, Berta, Yolande wrote:

}
} Dear Microscopists,
}
} We have a chunk of beryllium, and we would like to know the thickness of the
} oxide layer that forms on the metal within a given time interval. SEM hasn't
} shown the oxide layer, so now we are thinking that maybe a TEM cross-section
} specimen would be the thing to try.
}
} Has anyone prepared beryllium for TEM before? How? Ion milling?
} Jet-polishing? How was it cut to keep the dust at a minimum?
}
} Yolande Berta
} School of Materials Science and Engineering
} 778 Atlantic Dr.
} Atlanta, GA 30332-0245
} (404)894-2545
} yolande.berta-at-mse.gatech.edu
}




From: Nina S Allen :      nallen-at-unity.ncsu.edu
Date: Sat, 2 Mar 1996 11:31:16 -0500 (EST)
Subject: Re: Azide and plant cell ultrastructure

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential

Azide inhibits the mitochondria and yes it has a profund effect on the
cytoskeleton and cytoplasmic streaming in stamen hair cells of Setcresea.
Read E. Tucker and N.S. Allen, 1986. Cell Motil and the cytoskel.
6:305-313.

On Fri, 1 Mar 1996, T. Page Owen Jr wrote:

}
} Does anyone know if adding sodium azide to a plant cell suspension
} culture (final concentration 0.02%) affect its ultrastructure?
}
} It was added 30 minutes before fixing the cells in 2.5% glutaraldehyde.
} Azide was used in order to stop cell activity at a specific time point.
} The main interest in the ultrastructure analysis is to look at the Golgi
} apparatus, cell wall, plasma membrane, and endoplasmic reticulum.
}
} If it does (or doesn't) affect the ultrastructure; How? Which
} organelles are affected the most? Are there references in the literature
} about it?
}
} Thanks in advance for your input.
}
} Manrique Rojas
} Page Owen
} Department of Botany
} Connecticut College
}
}




From: levin-at-ecsuc.ctstateu.edu (Martin Levin)
Date: Sun, 3 Mar 1996 14:28:18 -0600
Subject: Replacement Bulbs

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I received a posting asking about replacement bulbs for some piece of
equipment. Unfortunately, before I completely digested the message, I
deleted it (I guess the hand is really quicker than the eye). I have an
excellent source for bulbs of all kinds, even those that are no longer
manufactured. You might want to give them a call to see if they have what
you are looking for. They are:

Bulbs Only
954 Queen Street
Southington, CT 06489
(203) 621-0213

I might add that they are extremely helpful and ship the next day.

Martin A. Levin
Department of Biology
Eastern Connecticut State University
Willimantic, CT 06226
Phone: (860)465-4324 FAX: (860)465-5213






From: Peter Baron :      baron-at-globalnet.co.uk
Date: Sun, 3 Mar 1996 17:07:37 GMT
Subject: List

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unsubscribe Peter Baron






From: Sarka Lhotak :      lhotaks-at-fhs.csu.McMaster.CA
Date: Sun, 3 Mar 1996 11:48:04 -0500 (EST)
Subject: Re: Immunohistochemistry in plastic

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On 25 Feb 1996 VFN6T-at-DMT03.mcc.Virginia.EDU wrote:

} Dear Colleagues,
} I am using plastic-embedded (Lowicryl or JB-4) sections of fetal kidneys
} and would like to use standard avidin-biotin-peroxidase techniques for
} immunohistochemistry. To date, we are unable to achieve specific staining.
} We have tried two different etching protocols and a few of the JB-4 (but none
} of the Lowicryl) sections survive, but still no staining. Most references
} give little information regarding incubation times, etc. Can anyone help me} trouble-shoot or point me towards a good reference? Thanks in advance.
} Victoria F. Norwood, M.D.
} Department of Pediatrics
} University of Virginia
} vfn6t-at-dmt03.mcc.virginia.edu
}
}
Dear Victoria,
A failure to immunolabel is usually due to the alteration of the
antigenic conformation during tissue processing. Each antigen/antibody
combination requires different conditions (fixation, incubation times,
choice of resin etc.) which have to be determined by trial and error
approach. Some antibodies will never label. Though some monoclonal
antobodies label very nicely, chances are better with polyclonal antisera
(affinity purified) as they bind to several antigenic determinants of the
antigen and some might be preserved better than others.
The best reference would be the one using your particular
antibody. Others can give you only general directions for finding the
optimal protocol (e.g. Hyatt's three volumes of Immunogold labelling -I
don't have the exact reference here, sorry).
First, I would try to stain paraffin sections, or even better,
cryostat sections. If there is no staining there, chances are that it will
not work in resin. However, if this is successful, you can use it to
determine the optimal concentration and incubation time and then proceed
to Lowicryl or LR White sections (I have no experience with JB4). You
should not need to etch them. Some antigens, however, require enzyme
digestion (trypsin or pepsin). Again this you would try on paraffin or
cryosections first. You might need to experiment with fixation. For TEM, I
usually start with three fixation protocols: 4% paraformaldehyde (PF), 2%
PF+ 0.1 % glutarladehyde (GA) or 2% GA to find the optimal tissue
preservation vs. labelling conditions.
I hope this will help a bit. These methods can be very frustrating
at times. You may contact me directly at my E-mail address if you need
more details.

Sarka Lhotak
lhotaks-at-fhs.mcmaster.ca
EM Facility, McMaster University
Hamilton, Ontario





From: Ross Davidson :      davidson-at-surf.ssw.uwo.ca
Date: Sun, 3 Mar 1996 19:15:05 -0500
Subject: Heavy Metals in Animal Tissues

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Message-Id: {199603040015.TAA22012-at-surf.ssw.uwo.ca}

Dear Microscopists:

We have recently been approached with an interesting analytical
problem concerning the detection of trace ( {200 ppm) levels of heavy
elements in animal
tissue. Elements of interest include selenium,nickel,arsenic,copper and many
others. The samples will be presented as thin (5 um) slices on a glass slide
substrate. Most of our work involves non-biological samples and this job
represents an interesting challenge.
We have available a Cameca 3f SIMS to carry out this work. We plan
to carry out depth profiles and also SIMS imaging.
Can anyone provide some useful tips in tackling this problem?
Also,is there any other techniques available that could also address this
question?
Any suggestions would be greatly appreciated.

Sincerely,
Ross Davidson Phone: 519-661-2173
Surface Science Western FAX: 519-661-3709
Western Science Centre, Rm G1, E-mail: davidson-at-surf.ssw.uwo.ca
University of Western Ontario Home Page: http://www.uwo.ca/ssw/
London, Ontario,
Canada N6A 5B7





From: HANS SLUIMAN :      RBG_3/HANS
Date: Sat, 2 Mar 1996 12:57:16 BST
Subject: Re: (Fwd) TEM;phosphate ppt in seawater

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Forwarded message:

} a user brought me a sample of gelatinous unicellular algae she
} preserved in 2% phosphate buffered glutaraldehyde. Unfortunately, it was a
} marine sample, so when she fixed it, the salts in the seawater
} precipitated.


I would suggest trying to dissolve the precipitate by diluting the
buffer. The combination of phosphate buffer and seawater is clearly
not a good one. In the past I always used glutaraldehyde in seawater
as a primary fixative for marine algae. I suspect it's the calcium in
the seawater who is to blame.


She can't repeat the experiment. Any way to salvage her
} sample--i.e., separate the cells from the ppt, or get the salts back into
} soln without detroying the cells? We appreciate that the precipitation may
} have ruined her cell preservation as well, but we won't know for sure till
} we can embed and section.

Alternatively one may keep part of the samples and proceed with
embedding and sectioning as usual, without trying to remove the
precipitate. Sectioning may be a bit difficult but some useful
results may be possible to obtain. I wouldn't use a diamond knife in
this case, though.

Yours sincerely

Dr Stephan Helfer, SSO
Mycologist / Plant Pathologist

Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
Scotland UK

http://www.rbge.org.uk

phone: +44 (0)131 552 7171 ext 280
or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
fax: +44 (0)131 552 0382
============================
1896
* BRITISH
* MYCOLOGICAL
* SOCIETY
1996
A century of fungal science
============================




From: knecht-at-uconnvm.uconn.edu (David Knecht)
Date: Mon, 4 Mar 1996 08:20:26 -0600
Subject: Hanging Drop slides

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Can someone point me to some information about how to use a hanging drop
slide. This should be obvious I guess, but I am sure there are some little
tricks that we would rather not have to figure out the hard way. Thanks-
Dave

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu






From: Dwight Beebe :      beebed-at-ere.umontreal.ca
Date: Mon, 4 Mar 1996 10:11:04 -0500 (EST)
Subject: Affinity-purified Ab

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Good morning,
I've recently been trying to do immunolocalization at the EM
level with affinity-purified Ab. My problem is that the purified Ab's
are in an ammonium thiocyanate solution that chews up both the Ni grids
and the sections (LR White). I know it's the solution because the
polyclonal serum immunos are fine. Does anyone with experience purifying
Ab's and using them for TEM immuno care to chime in with some pertinent
advice? I'd appreciate hearing any and all suggestions. Many thanks!

Dwight Beebe
Institut de recherche en biologie vegetale beebed-at-ere.umontreal.ca
Dept. de sciences biologiques Voice:514-872-4563
Universite de Montreal FAX:514-872-8496
4101, rue Sherbrooke est
Montreal, PQ H1X 2B2 Canada





From: kennedy-at-nsi.edu (grace kennedy)
Date: Mon, 4 Mar 1996 08:28:45 -0800
Subject: plastic immuno

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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Re: Immunostaining of plastic (JB-4, LRWHite) sections. According to the
circular accompanying the LR White resin, avidin-biotin binding is masked
by the plastic--they recommend using a PAP technique instead. The same may
be true with the methacrylate resins too, so your problem could conceivably
be caused by your choice of embedding medium. Needless to say, the advice
to examine other aspects of the system is of course wise especially if
you've never tried this specific detection using another preparation
technique. I found it to be amazing what antigens can really withstand
once the plastic masking problem has been solved.... Good luck. Grace
Kennedy






From: Liang, Long :      LLIANG-at-is.arco.com
Date: 04 Mar 1996 09:56:09 CST
Subject: EPMA -- N in graphite

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Message-Id: {MACMS.LLIANG.584314100096064FMACMS-at-IS.ARCO.COM}

Dear Microscopists,

I am trying to analyze graphite grains that are scattered in a sample
for their nitrogen contents. The mass absorption coefficient for
nitrogen Ka line in the carbon matrix is very very high (23,586).
Moreover, the nitrogen is a minor or trace element in the graphite.

Is this an impossible task for the probe analysis ? Are there any other
techniques can to used to complete this task ? Thanks.

Long Liang
ARCO EPMA/SEM Lab







From: CASORIA-at-FOZZIE.SSEC.HONEYWELL.COM
Date: Mon, 4 Mar 1996 12:27:59 -0600 (CST)
Subject: Benchmarking other semiconductor labs

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Fellow Microscopists,
We are trying to locate other analytical laboratories that might be interested
in benchmarking. Specifically, we would like to benchmark types of services
offered, size, education and expertise of staff, cycle-time, and SPC.
We are an semiconductor facility but may find acceptable benchmarks with labs
that provide .services to other than semiconductor houses.
Thanks!
Please reply to:
Carolyn Casoria
Honeywell/SSEC
-at-
casoria-at-ssec.honeywell.com




From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Mon, 4 Mar 1996 11:19:08 -0400 (EDT)
Subject: Hitachi 200kV TEM

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X-NUPop-Charset: English


Due to a consolidation of two microscopy facilities, we would like
to reluctantly part with the following excess instrument which is in
excellent condition and under service contract:


HITACHI H800/810 200 kV TEM
LaB6 electron gun
Motorized goniometer stage
SEM/STEM capabilities
Tracor/Northern (Noran) 5402 energy dispersive X-ray microanalysis
system with a 8502/S Advanced Image Analysis system
Special specimen holder for X-ray analysis of sectioned specimens
Special specimen holder for X-ray analysis of bulk specimens
Rotation/tilt specimen holder for grids.

Shipping the instrument is buyer's responsibility.

BONUS: If the purchase is completed before May 15th, 1996, a
HITACHI HHS-2R SEM (in storage) will be offered free of
charge to the H800 buyer.

Please contact me directly if interested.

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology &
Director, Cornell Integrated Microscopy Center
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: SNYDER, JOSEPH :      JTS-at-rrccorp.mhs.compuserve.com
Date: 04 Mar 96 14:27:24 EST
Subject: Wrong Subscription

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I have inadvertently given the wrong email address during my subscription
attempt.

Please change: JTS-at-rrc.mhs.compuserve.com

to: JTS-at-rrccorp.mhs.compuserve.com

You have my sincere apologies if this has caused you any problems.

Joe Snyder






From: SNYDER, JOSEPH :      JTS-at-rrccorp.mhs.compuserve.com
Date: 04 Mar 96 15:12:32 EST
Subject: Desubscribe

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Please desubscribe: JTS-at-rrc.mhs.compuserve.com






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Mon, 4 Mar 1996 09:09:09 GMT
Subject: Re: Digest

Contents Retrieved from Microscopy Listserver Archives
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} Is a digest now available for the Microscopy list? Thanks for any information.
}
}
} Blystone in Texas
}
} **********************
} ROBERT V. BLYSTONE PHONE:(210)736-7243
} DEPARTMENT OF BIOLOGY FAX:(210)736-7229
} Trinity University E-Mail:RBlyston-at-Trinity.edu
} 715 Stadium Drive
} San Antonio, TX, 78212
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

We currently archive items of interest to biologists. It is not yet
"searchable" but will be soon. It can be found at :

http://www.biotech.ufl.edu/~emcl/tips.html
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 4 Mar 1996 14:02:45 EST
Subject: Re: Hanging Drop slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To: Dr. David Knecht

To make a hanging drop slide: You will need a depression slide, a
square coverslip, some petrolatum, and the liquid suspension of what
you wish to view.

Place a small spot of petrolatum on each of the 4 corners of the
coverslip. Place a drop of your suspension in the center of the
coverslip. Invert a depression slide over the drop, allowing the
petrolatum to attach the coverslip to the depression slide. Quickly
(but carefully) invert the slide so that the coverslip is oriented
"up", and the drop is hanging into the slide depression.

That's all there is to it.

W. L. Steffens, Ph.D



Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 4 Mar 1996 14:02:29 EST
Subject: Re: Hanging Drop slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To: Dr. David Knecht

To make a hanging drop slide: You will need a depression slide, a
square coverslip, some petrolatum, and the liquid suspension of what
you wish to view.

Place a small spot of petrolatum on each of the 4 corners of the
coverslip. Place a drop of your suspension in the center of the
coverslip. Invert a depression slide over the drop, allowing the
petrolatum to attach the coverslip to the depression slide. Quickly
(but carefully) invert the slide so that the coverslip is oriented
"up", and the drop is hanging into the slide depression.

That's all there is to it.

W. L. Steffens, Ph.D



Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: Beverly E Maleeff
Date: 4 Mar 96 11:53:08 EDT
Subject: March PSM Meeting Notice

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Message-Id: {9603042108.AA1032-at-pho903.sbphrd.com}
To: microscopy {microscopy-at-Sparc5.Microscopy.Com}
{Beverly_E_Maleeff-at-sbphrd.com}

Philadelphia Society for Microscopy (PSM) March Meeting

When: Tuesday, March 12, 1996

Where: Laboratory for the Research of Science and Materials (LRSM),
on the campus of the University of Pennsylvania, 33rd and Walnut Sts.,
Philadelphia, PA

Program: 5:30 PM Social hour
6:30 PM Dinner Members $12.00, Non-members $15.00
7:30 PM Speaker

Speaker: Dr. Roberto F. Nicosia, Dept. of Pathology,
Medical College of Pennsylvania and Hahnemann University
Philadelphia, PA

"The Cell Biology of Blood Vessel Growth: Light microscopic,
immunohistochemical and ultrastructural studies"

Reservations will be taken by Ms. Pat Overend at the University of Pennsylvania,
215/898-8337. Deadline for dinner reservations is Friday, March 8.
Cancellations
must be received no later than 5:00 PM, March 11, 1996.

If you have any questions regarding the meeting please contact Rollin Lakis at
the
University of Pennsylvania, 215/898-8718.






From: VOS-at-CSSS.la.asu.edu
Date: Mon, 4 Mar 1996 17:42:53 -0700 (MST)
Subject: unsubscribe

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Please unsubscribe. Thank you




From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 4 Mar 1996 14:02:36 EST
Subject: Re: Hanging Drop slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To: Dr. David Knecht

To make a hanging drop slide: You will need a depression slide, a
square coverslip, some petrolatum, and the liquid suspension of what
you wish to view.

Place a small spot of petrolatum on each of the 4 corners of the
coverslip. Place a drop of your suspension in the center of the
coverslip. Invert a depression slide over the drop, allowing the
petrolatum to attach the coverslip to the depression slide. Quickly
(but carefully) invert the slide so that the coverslip is oriented
"up", and the drop is hanging into the slide depression.

That's all there is to it.

W. L. Steffens, Ph.D



Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Mon, 4 Mar 1996 19:31:07 -0600
Subject: Re: Air Dried Bacteria?

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Message-Id: {v02120d00ad6148d6ed4b-at-[128.174.107.186]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I've had excellent results air drying from hexamethylsilizane at
room temperature. Peldri will also work if you've got any left. Filtering
through a filter works fine.
But what % size change are you looking for? Recall the measuring
things, especially biological specimens in the SEM is dicey at best--and
can be grossly misleading.
Phil Oshel

} Hi All:
}
} I have been asked to examine, using the SEM, some bacteria that have been
} exposed to a drug solution. The interest is in whether the exposure to the
} drug solution has altered the bacterial shape and size (mostly size) and
} thus effect its retention on a filter. I propose to simply filter the
} bacterial suspension onto a polycarbonate membrane, air dry, mount, sputter
} coat, and examine.
} Damian Neuberger
} Email: neuberd-at-baxter.com

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 4 Mar 1996 14:03:03 EST
Subject: Re: Hanging Drop slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To: Dr. David Knecht

To make a hanging drop slide: You will need a depression slide, a
square coverslip, some petrolatum, and the liquid suspension of what
you wish to view.

Place a small spot of petrolatum on each of the 4 corners of the
coverslip. Place a drop of your suspension in the center of the
coverslip. Invert a depression slide over the drop, allowing the
petrolatum to attach the coverslip to the depression slide. Quickly
(but carefully) invert the slide so that the coverslip is oriented
"up", and the drop is hanging into the slide depression.

That's all there is to it.

W. L. Steffens, Ph.D



Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 4 Mar 1996 14:03:07 EST
Subject: Re: Hanging Drop slides

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


To: Dr. David Knecht

To make a hanging drop slide: You will need a depression slide, a
square coverslip, some petrolatum, and the liquid suspension of what
you wish to view.

Place a small spot of petrolatum on each of the 4 corners of the
coverslip. Place a drop of your suspension in the center of the
coverslip. Invert a depression slide over the drop, allowing the
petrolatum to attach the coverslip to the depression slide. Quickly
(but carefully) invert the slide so that the coverslip is oriented
"up", and the drop is hanging into the slide depression.

That's all there is to it.

W. L. Steffens, Ph.D



Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: GREG VAUGHN MARTIN :      gmartin-at-welchlink.welch.jhu.edu
Date: Mon, 4 Mar 1996 13:53:53 -0500 (EST)
Subject: Re: Affinity-purified Ab

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Dwight --
Try dialyzing the affi-pure Ab into Tris-Buffered Saline. I use
10mM Tris, 500mM NaCl, pH 7.2 for use on LRGold sections. Avoid
phosphate as it causes lead stain pption. Good Luck,

Greg Martin
Dept. of Cell Biology and Anatomy
Johns Hopkins School of Medicine

On Mon, 4 Mar 1996, Dwight Beebe wrote:

} Good morning,
} I've recently been trying to do immunolocalization at the EM
} level with affinity-purified Ab. My problem is that the purified Ab's
} are in an ammonium thiocyanate solution that chews up both the Ni grids
} and the sections (LR White). I know it's the solution because the
} polyclonal serum immunos are fine. Does anyone with experience purifying
} Ab's and using them for TEM immuno care to chime in with some pertinent
} advice? I'd appreciate hearing any and all suggestions. Many thanks!
}
} Dwight Beebe
} Institut de recherche en biologie vegetale beebed-at-ere.umontreal.ca
} Dept. de sciences biologiques Voice:514-872-4563
} Universite de Montreal FAX:514-872-8496
} 4101, rue Sherbrooke est
} Montreal, PQ H1X 2B2 Canada
}
}




From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 4 Mar 1996 13:45:26 EST
Subject: Re: Affinity-purified Ab

Contents Retrieved from Microscopy Listserver Archives
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To Dwight Beebe:

Antisera can be easily separated from undesirable salts by
dialysis...this is by far the simplest method. Obtain a short length
of dialysis tubing, soak it in water or buffer which will allow it to
be opened up into a tube. Tie off one end, fill with your antibody
solution, then tie off the other end. Immerse it into whatever
buffer you want to antibody to end up in (I suggest either TRIS
buffered or phosphate buffered saline. Use 1000X as much buffer as
antiserum, ie dialyse 1 cc of antibody against 1 liter of water for
24 hours, then change the buffer and continue for another 24 hours.
I recommend the last buffer change to contain 0.001 mM sodium azide
to retard microbial growth.

This will hopefully solve your problem.
W. L. Steffens, Ph.D



Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: rw9-at-psu.edu (Rosemary A. Walsh)
Date: Tue, 5 Mar 1996 00:18:25 -0500
Subject: Re: Air Dried Bacteria?

Contents Retrieved from Microscopy Listserver Archives
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"'Microscopy '" {Microscopy-at-Sparc5.Microscopy.Com}

At 3:57 PM 3/4/96 -0800, Neuberger, Damian wrote:
} Hi All:
}
} I have been asked to examine, using the SEM, some bacteria that have been
} exposed to a drug solution. The interest is in whether the exposure to the
} drug solution has altered the bacterial shape and size (mostly size) and
} thus effect its retention on a filter. I propose to simply filter the
} bacterial suspension onto a polycarbonate membrane, air dry, mount, sputter
} coat, and examine. This will supposedly work for the Gram positive microbes
} but may not work for the Gram negatives (they are said to have a much
} thinner wall and could shrink). An alternate approach is to heat fix the G-
} to a glass coverslip, sputter coat, and examine.
}
} Is this approach OK or is there a better way to do this, perhaps using a
} fixative? I would rather not have to go the route of CPD. Any suggestions
} will be greatly appreciated!!!
}
} Damian Neuberger
} Email: neuberd-at-baxter.com

Dear Damian,
One alternative to CPD is to filter, fix, dehydrate and follow the
final 100% ETOH dehydration with 3 baths of 100% HMDS
(Hexamethyldisilizane). Polycarbonate membranes handle the solvent very
well. They dry in minutes and you can quickly afix them to stubs and
sputter coat.
Rosemary WAlsh, EM Facility, PSU






From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 4 Mar 1996 15:33:16 -1000 (HST)
Subject: Pt/Ir coating for SEM

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In the absence of a means to coat biological specimens with Cr for
high(er) resolution SEM, we use the electron guns in our Balzers 400
freeze-fracture device to coat with various metals and carbon. Several
years ago Hans Ris suggested Pt/Ir/C coating, and we have had very good
results with this, although we have to constantly struggle with some
of the parameters. I am interested in hearing from others who use
this technique to see if we can compare notes. For example, should the
coating be done cold or at room temperature? We have had some success
under both conditions. What kinds of distances, angles, vacuums, etc.?

On a related note, we had been purchasing 80% Pt/20% Ir wire
and balling it up and melting it into drilled out C rods (supplied by
Bal-Tec), but this was a pain to do as compared with using the Pt
inserts made for the drilled rods. Bal-Tec would make the Pt/Ir pellets
to order, but at substantial cost. Larry Stevens at Stevens
Metallurgical Corp. has agreed to make the Pt/Ir pellets and/or rods at
a very reasonable price if there appears to be enough of an interest. I
would be like to hear from others who might buy Pt/Ir pellets so that we
might encourage Mr. Stevens to do so!

Aloha from sunny, warm Hawaii (and surf's up),
Tina
*****************************************
Tina (Weatherby) Carvalho *
Biological Electron Microscope Facility *
University of Hawaii *
(808) 956-6251 *
tina-at-ahi.pbrc.hawaii.edu *
http://www.pbrc.hawaii.edu/bemf/ *
*****************************************





From: Neuberger, Damian :      neuberd-at-engrnd.roundlake.baxter.com
Date: Tue, 5 Mar 1996 07:56:02 GMT+0200
Subject: Air Dried Bacteria?

Contents Retrieved from Microscopy Listserver Archives
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"Neuberger, Damian" {neuberd-at-engrnd.roundlake.baxter.com}



Hi All:

I have been asked to examine, using the SEM, some bacteria that have been
exposed to a drug solution. The interest is in whether the exposure to the
drug solution has altered the bacterial shape and size (mostly size) and
thus effect its retention on a filter. I propose to simply filter the
bacterial suspension onto a polycarbonate membrane, air dry, mount, sputter
coat, and examine. This will supposedly work for the Gram positive microbes
but may not work for the Gram negatives (they are said to have a much
thinner wall and could shrink). An alternate approach is to heat fix the G-
to a glass coverslip, sputter coat, and examine.

Is this approach OK or is there a better way to do this, perhaps using a
fixative? I would rather not have to go the route of CPD. Any suggestions
will be greatly appreciated!!!

Damian Neuberger
Email: neuberd-at-baxter.com

Hello Damian!

No fix and air drying will almost certainly change the shape and
size of the bacteria. If you wish to avoid critical point drying
you could try fixation followed by a CPD substitute such as
HMDS (hexamethyldisilazane), TMS (tetramethylsilane) or Peldri
(if available). Alternatively, freeze-drying, cryo-SEM or LVSEM
would be preferable to air-drying without fixation.

Regards,



Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)




From: CSEDAX-at-ARCRIDE.EDU.AR
Date: Tue, 5 Mar 1996 09:28 -0300
Subject: X-Ray Fluorescence: listserver and sources for buying supplies needed.

Contents Retrieved from Microscopy Listserver Archives
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Dear microscopists,

besides SEM and SEM-EDS analysis we are doing XRF
services since not so long ago. We know this may not be the forum to asking
about XRF but, we have experienced the kindness of many of you out there.

Our questions are:

* do any of you know of any listserver for X-Ray Fluorescence Spectroscopists
to discuss ideas with?

* we're desperately needing mylar or polypropilene film for doing XRF
analysis, do you know a source for XRF supplies ? We'd appreciate very
much receiving postal or e-mail addresses or fax to contact them as soon
as possible.

We thank all of you for your attention,

Angel Di Giandomenico
cnono-at-arcride.edu.ar

Centre for Research and Development
Santa Fe - Argentina




From: James S MArtin :      James.S.Martin-at-williams.edu
Date: Tue, 5 Mar 1996 07:41:04 -0500 (EST)
Subject: field trips

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As part of an introductory course on microscopy, I am trying to set up
field trips so students can observe practical applications of acoustic
and scanning tunneling microscopy.

So ... I am looking for industrial, commercial or academic labs within
driving distance of Williamstown, MA that would be willing to spend some
time with a small group of eager undergraduates. Dates to be determined.

Thanks.

James Martin
Williams College




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Tue, 5 Mar 1996 09:03:58 GMT
Subject: Re: Air Dried Bacteria?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} To: "Neuberger, Damian" {neuberd-at-engrnd.roundlake.baxter.com}
} From: gwe-at-biotech.ufl.edu (Greg Erdos)
} Subject: Re: Air Dried Bacteria?
} Cc:
} Bcc:
} X-Attachments:
}
} }
} } Hi All:
} }
} } I have been asked to examine, using the SEM, some bacteria that have been
} } exposed to a drug solution. The interest is in whether the exposure to the
} } drug solution has altered the bacterial shape and size (mostly size) and
} } thus effect its retention on a filter. I propose to simply filter the
} } bacterial suspension onto a polycarbonate membrane, air dry, mount, sputter
} } coat, and examine. This will supposedly work for the Gram positive microbes
} } but may not work for the Gram negatives (they are said to have a much
} } thinner wall and could shrink). An alternate approach is to heat fix the G-
} } to a glass coverslip, sputter coat, and examine.
} }
} } Is this approach OK or is there a better way to do this, perhaps using a
} } fixative? I would rather not have to go the route of CPD. Any suggestions
} } will be greatly appreciated!!!
} }
} } Damian Neuberger
} } Email: neuberd-at-baxter.com
} }
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
}
} Get your bacteria to attach to a polylysine coated cover slip. Fix with
glut and osmium, dehydrate and dry via HMDS (two changes of
hexamethyldisilazane after 100% alcohol, then air dry finishing up with ten
minutes in a 60 C oven). Then sputter coat Will give you much better
results than heat fixing and air drying.
}
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: thsu-at-MSC.nthu.edu.tw (Tung Hsu) (by way of chandler-at-lamar.Colostate.EDU (John
Date: Tue, 5 Mar 1996 09:04:58 -0700
Subject: Air Dried Bacteria

Contents Retrieved from Microscopy Listserver Archives
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Chandler))

WANT TO BUY OR ACCEPT DONATION:
electron microscope

Usable JEOL JEM-100C, 100CX, 100S , or equivalent.
Must be in OK running condition.
Accessories, high resolution, etc. unimportant.
Will pay for shipping.
This is for my colleague studying polymer materials.
You may contact me if you have one to give away or have any lead.
Thank you in advance.

Tung Hsu
Material Science Center
National Tsing-hua University
Hsin-chu 30043
TAIWAN

telephone: 886-35-724951 (with answering machine)
886-35-715131 ext 5393
fax: 886-35-713208
E-mail: thsu-at-msc.nthu.edu.tw






From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Tue, 5 Mar 1996 12:29:18 -0500 (EST)
Subject: Used TEM for sale

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Recently, I sent a message to you all about a used TEM, to see if there
was any interest. I have had some, so here is the real offer.

We have a Philips 201c TEM for sale, it is approximately 20 years old.
It is functional, but lack of use has caused the vacuum to be less than
optimal. With a little TLC this scope could be an excellent workhorse.
The investigators who own the scope would be willing to sell it for
parts, but they want it removed from their facility, not just scavenged,
as they need to make room for a new scope.

Thanks in advance

Cheri Owen
Wayne State University
Detroit Neurotrauma Institute
Detroit, MI
(313)577-4648




From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Tue, 5 Mar 1996 12:29:18 -0500 (EST)
Subject: Used TEM for sale

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html




Recently, I sent a message to you all about a used TEM, to see if there
was any interest. I have had some, so here is the real offer.

We have a Philips 201c TEM for sale, it is approximately 20 years old.
It is functional, but lack of use has caused the vacuum to be less than
optimal. With a little TLC this scope could be an excellent workhorse.
The investigators who own the scope would be willing to sell it for
parts, but they want it removed from their facility, not just scavenged,
as they need to make room for a new scope.

Thanks in advance

Cheri Owen
Wayne State University
Detroit Neurotrauma Institute
Detroit, MI
(313)577-4648




From: Ian MacLaren :      MACLARIZ-at-novell2.bham.ac.uk
Date: 5 Mar 1996 16:14:27
Subject: TEM: preparation of ceramic fibres

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To: Microscopy-at-Sparc5.Microscopy.Com

Dear all,
Has anyone out there prepared non-conducting ceramic fibres for TEM, either
across or along axis? If so, how did you do it?
We have some mullite fibres we would like to look at and although we have
some ideas about how to prepare specimens we would appreciate any ideas or
feedback on this topic.

Thanks

Dr. Ian MacLaren,
IRC in Materials for High Telephone: 0121 414 3447
Performance Applications, FAX: 0121 414 3441
The University of Birmingham, email: I.MacLaren-at-bham.ac.uk
Birmingham B15 2TT,
England




From: Joe D Geller :      geller-at-world.std.com
Date: Tue, 5 Mar 1996 11:56:41 -0500 (EST)
Subject: Re: EPMA -- N in graphite

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Nitrogen in graphite can be detected to a concentration level of
approximately 0.08% in a relatively short amount of time using the LDE
type of metal crystal in a wavelength dispersive x-ray spectrometer
mounted on a electron probe microanalyzer. The spatial resolution would
be on the order of 1 or 2 micrometers.

If you don't have this type of instrumentation there are commercial
laboratories offering this service, including ours.

Joe Geller
Geller MicroAnalytical Laboratory
426e Boston ST.
Topsfield, MA 01983-1216
508 887-7000 fax 887-6671
On 4 Mar 1996, Liang, Long wrote:

} Dear Microscopists,
}
} I am trying to analyze graphite grains that are scattered in a sample
} for their nitrogen contents. The mass absorption coefficient for
} nitrogen Ka line in the carbon matrix is very very high (23,586).
} Moreover, the nitrogen is a minor or trace element in the graphite.
}
} Is this an impossible task for the probe analysis ? Are there any other
} techniques can to used to complete this task ? Thanks.
}
} Long Liang
} ARCO EPMA/SEM Lab
}
}
}




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From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Tue, 5 Mar 1996 17:30:19 -0600
Subject: LM: lab manuals, textbooks

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I am looking for good reference books and lab manuals containing protocols
for use in a course on histotechnique\microtechnique. Anyone have some good
recent and classical books (Title, Author, Publishers)? Many thanks.

#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: LSFY69A-at-PRODIGY.COM (MR KARL H BERGER)
Date: Mon, 04 Mar 1996 20:21:53 EST
Subject: subscribe

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LSFY69A -at- prodigy.com





From: wschweitzer-at-access.ch (Wolf Schweitzer)
Date: Wed, 6 Mar 1996 02:25:07 GMT
Subject: Re: external computer drives

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concerning SyQuest Compatibility:

I am using an external SyQuest 44MB Drive:

- no problems on MacPlus, SE/30, IIcx, Quadra 700/800/900, PowerPC 8500
- STOPS MACHINE from booting on Quadra 900 with Apple PPC upgrade card !!

Greetings from Switzerland, Wolf Schweitzer.



----------------------------------------------
Wolf Schweitzer, MD - CH 8596 Scherzingen
E-Mail wschweitzer-at-access.ch
http://www.access.ch/whoiswho/wschweitzer.html






From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Tue, 5 Mar 1996 20:08:19 -0600
Subject: Re: LM: lab manuals, textbooks

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Mime-Version: 1.0
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John,
Best books I know of are John Kiernan's _Histological and
Histochemical Methods_ and the Biological Stain Commission's _Staining
Procedures_ (9th or 10th [or...?] edition). I don't have Kiernan's book to
hand, but if he doesn't respond, I can send you the correct info. (Same for
_Staining Procedures_.) I heard there was a good EM book with some
procedures written by some guy in southern Illinois...
Phil Oshel

} I am looking for good reference books and lab manuals containing protocols
} for use in a course on histotechnique\microtechnique. Anyone have some good
} recent and classical books (Title, Author, Publishers)? Many thanks.
}
} #############################################################################
} John J. Bozzola, Ph.D., Director
} Center for Electron Microscopy
} Southern Illinois University
} Carbondale, IL 62901-4402
} U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} #############################################################################

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: Dirk.Voeste-at-rz.ruhr-uni-bochum.de
Date: Wed, 6 Mar 1996 17:07:41 +0000
Subject: subscribe

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Dear Sirs,

please subscribe,

Dr. D. Voeste




From: Andrews Lab
Date: 3/5/96 6:17 PM
Subject: >Question about HVEM

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Message-Id: {n1386012229.51338-at-QuickMail.Yale.edu}



--------------------------------------
Do I have to caot the grids ?
Thanking you in advance for the suggestions, i am looking forward to try this
technique
annette Bakker







From: Daniel L. Callahan :      dlc-at-owlnet.rice.edu
Date: Wed, 6 Mar 1996 11:16:09 -0600 (CST)
Subject: silicon transmission

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Message-Id: {199603061724.MAA18470-at-thomas.ge.com}


Good Day:

Is there an available color-spectrum illustrating the color changes
undergone in silicon as it is thinned. This effect is routinely used for
final polishing but I have never actually seen a quanitative correlation
for just how thin 'yellow' transmitting silicon is, for example. I
presume it is also orientation dependent, but don't know to what degree.

A color-guide would make a very useful HTML page for new users.

Daniel L. Callahan
Department of Mechanical Engg. and Materials Science
Rice University
dlc-at-owlnet.rice.edu




From: Secretaire-at-sc.ucl.ac.be
Date: Wed, 6 Mar 1996 18:19:51 +0100
Subject: LM audio visual support

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Good morning
I am looking for audio visual aids (video, CDŠ) for illustrating
theory and/or possibilities of all kinds of LM. This is for use in a
course on investigation methods. Recent reference books or booklets edited
by manufacturers are also welcome.
I'd appreciate your suggestions. Many thanks!

Prof. Jean-Pierre Auquiere
Universite catholique de Louvain (UCL)
Secr. Acad. Faculte des Sciences Unité de botanique generale (BOTA)
Place des Sciences, 2 Place Croix du Sud, 4
B-1348 Louvain la Neuve, Belgium B-1348 Louvain la Neuve, Belgium
tel + 32 10 47 86 78 tel + 32 2 764 51 22
fax + 32 10 47 28 37 fax + 32 2 764 72 55
E mail secretaire-at-sc.ucl.ac.be E mail auquiere-at-bota.ucl.ac.be






From: Doug Keene :      DRK-at-SHCC.ORG
Date: Wed, 06 Mar 1996 14:22:10 -0800 (PST)
Subject: photographic processors

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Considering that photography is still so important to our profession, it is
likely that in this group are a few critical individuals with personal
experiencee using dry-to-dry black and white photographic processors. Would
anyone care to recommend a counter-top processor that they are happy with? I
wouldn't object to knowing which ones to stay away from either.

Many thanks,

Doug Keene
Shriners Hospital for Crippled Children
Portland, Oregon




From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Wed, 6 Mar 1996 08:53:30 -0800 (PST)
Subject: Re: LM: lab manuals, textbooks

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X-Sender: glenmac-at-homer09.u.washington.edu


A useful introductory text on LM sample processing and staining methods is
Animal Tissue Techniques,
Gretchen L. Humason, W.H. Freeman, I believe the 3 rd edition is the
most recent.

Regards,

Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu


On Tue, 5 Mar 1996, John. J. Bozzola wrote:

} I am looking for good reference books and lab manuals containing protocols
} for use in a course on histotechnique\microtechnique. Anyone have some good
} recent and classical books (Title, Author, Publishers)? Many thanks.
}
} #############################################################################
} John J. Bozzola, Ph.D., Director
} Center for Electron Microscopy
} Southern Illinois University
} Carbondale, IL 62901-4402
} U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} #############################################################################
}
}
}




From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Wed, 6 Mar 1996 17:53:32 -0600
Subject: MSA/MAS/MSC-SMC Comprehensive Info Location

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If you would like to access the most up to date information
about the joint meeting between MSA, MAS & MSC-SMC
you can get everything on-line on the WWW.

This includes:
Scientific Program
Short Courses
Posters
Special Awards
Financial Support
Social Events
How to submit manuscripts
Registration Forms
Dates, Times, Deadlines, etc...

Just access the MSA WWW site at

http://www.msa.microscopy.com

Look for the hot links to "Registration & Meeting Info - 1996s"



Cheers... Nestor

Your Friendly Neighborhood SysOp
&
the Microscopy & Microanalysis Program Chair for 1996






From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 6 Mar 1996 12:09:03 -0500 (EST)
Subject: Re: TEM: preparation of ceramic fibres

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} Has anyone out there prepared non-conducting ceramic fibres for TEM, either
} across or along axis? If so, how did you do it?

Dear Ian,
This may or may not be applicable, but with the high-voltage EM
(lower interaction cross-section) and very low beam currents, we have been
able to image non- or poorly-conducting specimens without charging or heat-
ing problems. If this holds true for your instrument (e.g. you have an
IVEM), the advantage is that there may be no preparation required; the
disadvantage is that with these low doses you will either need a very sen-
sitive recording medium--LoDose or other x-ray film, image plates or in-
tensified or slow-scan CCD--or long exposures. The large grain or speci-
men drift could make the images useless. Good luck.
Yours,
Bill Tivol




From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Wed, 6 Mar 1996 10:44:27 -0800
Subject: Call for papers, 4th Annual Microscopy Colloquium

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4th ANNUAL MICROSCOPY COLLOQUIUM

held jointly with:

California State University
and the
California Society For Microscopy

May 4 & 5, 1996
San Diego State University

REGISTRATION AND ABSTRACTS

The abstracts will be compiled into the "Proceedings of the Fourth Annual
Microscopy Colloquium" and will be published in Microscopy Research and
Technique. Abstract information and details on email submission of
abstracts available on request.

Abstracts must be received by March 29,1996

Registration fees (by March 29):
$35/regular, $10/student, $50/vendor

Late Registration fees (March 29):
$45/regular, $10/student, and $60/vendor.

Please send the enclosed registration form whether or not you intend to
make a presention

PLATFORM AND POSTER PRESENTATIONS

Papers discussing any aspects of microscopy in cell, developmental,
and structural biology, or material and geological sciences are welcome.
Microscopy techniques papers and student presentations are particularly
encouraged.
Platform presentations will be held on Saturday, May 4th and Sunday
morning, May 5th. Posters will be displayed Saturday, May 4, with authors
present.

PROGRAM

Friday - May 3:
4:30 p.m. Business meeting for CSU delegates at Comfort Inn, La Mesa

Saturday - May 4:
8:00 a.m. Registration/Aztec Hall
San Diego State University
9:00 a.m. Opening remarks
9:15 a.m. Platform presentations
10:30 a.m. Coffee break
10:45 a.m. Platform presentations
12:00 p.m. Buffet lunch
1:30 p.m. Platform presentations
3:00 p.m. Coffee break
3:15 p.m Poster presentations
4:00 p.m. Poster authors present
4:45 p.m. Reception/ Banana Quad
Tour of the SDSU EM Facility
6:00 p.m. Banquet
7:30 p.m. Keynote address

Sunday-May 5
9:00 a.m. Platform presentations
10:45 a.m. Coffee break
11:00 a.m. Panel discussion
"Remote Access To Instrumentation"
12:00 p.m. Closing remarks



Registration Form
CSU Microscopy Colloquium
May 4 & 5, 1996
San Diego

_____________________________________________________
Name

_____________________________________________________
Address

_____________________________________________________
City State Zip

______________________ ________________________
Phone e-mail

Presentation:
O Platform O Poster O No Presentation

Meal Preference:
O Chicken O Fish O Vegetarian

Make checks payable to "EM Facility/Colloquium"

SEND TO:

Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Drive
San Diego, CA. 92182-4614


(619) 594- 4523
sbarlow-at-sunstroke.sdsu.edu

HOUSING:

The Comfort Inn, La Mesa

2 miles from SDSU

Housing for the Colloquium will be available at the Comfort Inn, 8000
Parkway Drive, La Mesa. The room rate is $42.00 per night, plus tax. You
must make your own reservations. Please call (619) 698-7747 and specify
that you will be attending the Microscopy Colloquium.

Shuttle Service:

Airport shuttle service is available from several companies. We
recommend the California Sunshine Shuttle Service at $12 per person to SDSU
and the Comfort Inn. Please make advanced reservations by calling collect
(619) 443-7900, 6 a.m. to 9 p.m. PST and specify that you will be attending
the Microscopy Colloquium. A free shuttle will provide limited tranport
between SDSU and the Comfort Inn Saturday and Sunday.


Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego, CA 92182-4614
phone: (619)594-4523
fax:(619)594-5676
email:sbarlow-at-sunstroke.sdsu.edu






From: Ian MacLaren :      MACLARIZ-at-novell2.bham.ac.uk
Date: 7 Mar 1996 11:13:37
Subject: Re: TEM: preparation of ceramic fibres

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"Childress, Charles P. (Chip)" {cpc4-at-NIOBBS1.EM.CDC.GOV} ,
daughecc-at-UCBEH.SAN.UC.EDU, LLOYDPF-at-ml.wpafb.af.mil
Message-Id: {960306140912.626-at-cliff.ml.wpafb.af.mil.0}

Thanks to all who have responded speedily to my enquiry about preparation of
ceramic fibres for TEM. I will be trying some of these ideas in the next
few days.

Thanks again

Ian MacLaren,
IRC in Materials for High Telephone: 0121 414 3447
Performance Applications, FAX: 0121 414 3441
The University of Birmingham, email: I.MacLaren-at-bham.ac.uk
Birmingham B15 2TT,
England




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 7 Mar 1996 09:17:20 GMT
Subject: Re: photographic processors

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} Considering that photography is still so important to our profession, it is
} likely that in this group are a few critical individuals with personal
} experiencee using dry-to-dry black and white photographic processors. Would
} anyone care to recommend a counter-top processor that they are happy with? I
} wouldn't object to knowing which ones to stay away from either.
}
} Many thanks,
}
} Doug Keene
} Shriners Hospital for Crippled Children
} Portland, Oregon
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } We have
been using the Ilford 2150 RC for over a year, maybe two by now.
The results are quite good, however there have been mechanical problems with
it from the get go. We had to replace bearings early on and have had to
dismantle and re-assemble the rollers over and over to keep it from
squeaking and sqealing and grinding. So it has been a mixed bag for us. We
do about 10,000 prints a year and it has been a real time-saver too
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Thu, 7 Mar 1996 10:04:19 -0500
Subject: Publisher of S. Klosevych Series?

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Does anyone know who published, and the current source, of a series of
tutorial papers called "Microscopy and Photomicrography"? They were
written by Stanley Klosevych of the University of Ottawa.

Thank you in advance,
David Rothbard

--
Institute of Paper Science and Technology






From: kennedy-at-nsi.edu (grace kennedy)
Date: Thu, 7 Mar 1996 08:19:37 -0800
Subject: microscope disposal

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Fellow microscopists, what is the best and most efficient way to get rid of
an old TEM?? I see many for sale, etc., but have never seen replies. We
have an old Hitachi H-500 STEM, maintained by Hitachi until last year when
the $ ran dry. It's been used strictly as a biological TEM for the last 7
years or so. I would hardly thnk it would pay anyone to have it
disassembled and shipped, and it can't be left in place. Thanks in advance
for any input.






From: Elinor Solit :      cambrex-at-world.std.com
Date: Thu, 7 Mar 1996 12:14:11 -0500 (EST)
Subject: Re: MSA Meeting Info. Wanted

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Lee,

The good people at the MSA office will be glad to help. Ask for Sharon or
Larry. Their number is 800-538-3672

See you at MSA.

Ellie Solit
The Microscope Book

On Wed, 6 Mar 1996, Lee Wagstaff wrote:

} Hi Gang,
}
} Anyone out there know where to obtain some comprehensive info on the MSA meeting coming
} up this August?? Any assistance would be appreciated. Thanks. Lee.
}




From: Linda Fox :      lfox1-at-wpo.it.luc.edu
Date: Thu, 07 Mar 1996 10:12:24 -0600
Subject: photographic processors

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Message-Id: {199603071725.LAA11394-at-Sparc5.Microscopy.Com}

The best processor out there is the Mohrpro. We have been using one
for about 8 years and recommend it when ever we can. It is easy to
use and maintain, 2 min. dry to dry, permanent (not stabilized) and
uses RC paper and Kodak Fixer. We do purchase the developer from
Mohr. The current cost for paper up to 8" width is $4295, the
Mohrpro8. and 14" width $4800, the Mohrpro14. (They do sell
rebuilt machines as well). They stand behind their product and I can
only say great things about Mohr.
For information contact:
Bob or Jim Jackson (tell them Linda at Loyola sent you!)
Mohr Enterprises
65 E. Palatine Rd. Suite 103
Prospect Heights, Il 60070
1-847-465-0048

Linda Fox Loyola University Medical School
lfox1-at-wpo.it.luc.edu





From: knoff-at-lipovx.lbl.gov (Laura Knoff)
Date: Thu, 7 Mar 1996 11:01:26 -0800
Subject: NCSM Meeting

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WHAT: The Spring meeting of the Northern California Society for Microscopy.

WHEN: Thursday, March 14 from 5:30 to 9:30 p.m.

WHERE: Genentech, 460 Point San Bruno Blvd., So. San
Francisco, Ca

Speakers: Dr. Wen Lu Li, Genentech -

"Microscopy as a Tool in the Development of Theraputic Molecules"
&
Dr. Ming Pan, Gatan -

"High Resolution Transmission Electron Microscopy of Zeolites"

The Northern California Society for Microscopy (NCSM) welcomes new
members. Anyone interested in microscopy of any type, from atomic force to
optical will benefit from joining the NCSM. We meet four times a year to
have dinner and hear about new developments in microscopy. Dues are very
reasonable at only $15 a year for regular members, and $8 for students. If
you are interested in joining, or knowing more about NCSM, please contact
Kent McDonald by phone at 510-642-2085 during normal business hours, or by
email at: klm-at-uclink4.berkeley.edu If you are interested in attending the
Mar. 14 meeting described above, please call 510-486-4088 no later than
Mar. 8 so we can reserve the appropriate number of dinners. Complimentary
drinks will be provided by MicroStar Diamond Knives and RMC and the dinner
will be $15 for members, $7.50 for student members. Please join us.








From: William R Oliver :      oliver-at-ipas4.afip.mil
Date: Thu, 7 Mar 1996 12:31:19 -0500 (EST)
Subject: Re: microscope disposal

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What about museums and such? I know that there are technology/medicine
museums scattered about which might have a use. I know that the National
Museum of Health and Medicine here at the AFIP has a microscope
collection, and I believe the Smithsonian might as well. I assume that
there would be other museums in the country which would also benefit from
such donations.

billo

On Thu, 7 Mar 1996, grace kennedy wrote:

} Fellow microscopists, what is the best and most efficient way to get rid of
} an old TEM?? I see many for sale, etc., but have never seen replies. We
} have an old Hitachi H-500 STEM, maintained by Hitachi until last year when
} the $ ran dry. It's been used strictly as a biological TEM for the last 7
} years or so. I would hardly thnk it would pay anyone to have it
} disassembled and shipped, and it can't be left in place. Thanks in advance
} for any input.
}
}
}




From: LSFY69A-at-PRODIGY.COM (MR KARL H BERGER)
Date: Wed, 06 Mar 1996 17:15:40 EST
Subject: microscope for sale

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Zeiss EM-109 for sale, good condition, $ 5,000.- or best offer.
Call 908-370-8082





From: LSFY69A-at-PRODIGY.COM (MR KARL H BERGER)
Date: Wed, 06 Mar 1996 17:15:40 EST
Subject: microscope for sale

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Zeiss EM-109 for sale, good condition, $ 5,000.- or best offer.
Call 908-370-8082





From: MelanieOwl-at-aol.com
Date: Thu, 7 Mar 1996 17:23:33 -0500
Subject: EDS Detectors for FE-SEM

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Is it necessary to get a 30 mm window for an EDS detector to be used on a
Schottky emitter FE-SEM in order to get enough counts, or is a 10 mm okay?

Thanks in advance,
Melanie Behrens
Texaco, Inc.
Fuels and Lubes Research Dept.
Beacon, NY
behrema -at- Texaco.com or MelanieOwl -at- aol.com




From: Deborah Holmberg :      dlholmberg-at-ucdavis.edu
Date: Thu, 7 Mar 1996 14:33:39 -0800 (PST)
Subject: Scanning 96, student volunteers

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Hello All,
The Scanning 96 Meeting in Monterey, CA on 9-12 April is looking for
students to monitor the sessions, ie. run the slide projectors, adjust
lights and hand out information. The student will have the registration
fee waived, in appreciation of helping. For information, please contact
me, or the Scanning 96 office at FAMS, Inc., PO Box 832, Mahwah, NJ
07430-0832. E-mail: fams-at-holonet.net
Thank you,
Debe Holmberg
USDA-ARS
916.752.9021
916.752-4604 (fax)




From: VCRVINCE-at-aol.com
Date: Thu, 7 Mar 1996 20:39:56 -0500
Subject: Si Colors

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Dan,

Si color changes related to us by Tom Cass in '81 when VCR GROUP designed the
first DIMPLER, D100 were:

Garnet10-12um, Wheaty brown 7-9um, yellow 4-6um, white 1-3um, fringes {1um


The basic dimpler design came from his lab at Hewlett Packard Deer Creek
Labs, EMSA Bullentin 10(1) 1980, p.66, Addendum 10(2) 1980, p.93.

The colors depend on the intensity and type of lighting, Si dimpled and
viewers color perception. Specimen thickness can be measured quickly and
accurately on the DIMPLER specimen platen with a good optical microscope.
This measurement technique is especially helpful with specimens that are not
light transmitting.

V. L. Carlino
VCR GROUP, Inc.







From: Steven Schwarz :      sschwarz-at-morgan.ucs.mun.ca
Date: Thu, 7 Mar 1996 19:14:08 -0330 (NST)
Subject: LH-zeiss parts

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Hi-

I have a Zeiss Universal 'M' microscope which is missing a few
parts, which are now VERY expensive (from Zeiss).

I require a REFLECTOR for a 'Vertical Illuminator IIC'; either a
H-PL, or H-PR will do. Only the reflector is required as I have the
housing. Does anyone have one of these ? Or does anyone know if it is
possible to 'make' one using a 'double sided semi-transparent mirror' ?


Many thanks

steve


==============================================================================
Steve Schwarz sschwarz-at-morgan.ucs.mun.ca
Dept. of Earth Sciences
Memorial University of Newfoundland
Newfoundland
CANADA
A1B 3X5
1-709-737-8142
-737-2589 FAX
******************************************************************************




From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Fri, 8 Mar 1996 10:47:20 +1100
Subject: Re: microscope disposal

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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Fellow microscopists, what is the best and most efficient way to get rid of
} an old TEM?? I see many for sale, etc., but have never seen replies. We
} have an old Hitachi H-500 STEM, maintained by Hitachi until last year when
} the $ ran dry. It's been used strictly as a biological TEM for the last 7
} years or so. I would hardly thnk it would pay anyone to have it
} disassembled and shipped, and it can't be left in place. Thanks in advance
} for any input.


Metal recyclers are an option. Taking parts home as unusual potplant
holders (bits of the column), paperweights (the lead glass), decorations
etc is fun. Perhaps a local school would like it.


Diana van Driel
Dept Ophthalmology C09
Sydney University 2006
NSW, AUSTRALIA






From: Stephen Edgar :      s.edgar-at-auckland.ac.nz
Date: Fri, 8 Mar 1996 10:54:49 NZS
Subject: Re: Publisher of S. Klosevych Series?

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} Does anyone know who published, and the current source, of a series
} tutorial papers called "Microscopy and Photomicrography"? They were
} written by Stanley Klosevych of the University of Ottawa.
}
} Thank you in advance,
} David Rothbard


Dear David,

I believe these tutorials have been published as a single volume, but
I do not know the details of that.

They were also published as a series of six papers in the Journal of
Biological Photography in 1974 and 1975. The details are:

Part I vol. 42:part 3, pp 123-131
Part II 42:4, 147-160
Part III 43:1, 30-38
Part IV 43:2, 51-69
Part V 43:3, 119-139
Conclusion 43:4, 187-188


About 24 degrees Celsius and not a cloud in the sky. Who needs Hawaii?


Regards

Stephen Edgar

Electron Microscope Unit, Pathology Department
School of Medicine
University of Auckland
Private Bag 92019
Auckland
New Zealand

email address: s.edgar-at-auckland.ac.nz
Phone : +64-9-3737599 extn 6473 (GMT + 12h)
Fax : +64-9-3737459




From: Roar Kilaas :      Roar_Kilaas-at-macmail2.lbl.gov
Date: 7 Mar 1996 13:44:34 U
Subject: Workshop on Quantitative HR

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Message-Id: {n1385903893.39621-at-macmail2.lbl.gov}

REGARDING Workshop on Quantitative HRTEM

Announcement of Workshop/Symposium
-------------------------------------------

The National Center for Electron Microscopy at the Ernest Orlando Lawrence
National Laboratory will be hosting a 3 day workshop on Quantitative High
Resolution
Transmission Electron Microscopy, April 18 - April 20, 1996.
This workshop is designed to address many important issues relating to
obtaining
quantitative information from experimental data in HRTEM. The workshop will
begin
with discussing the many steps required in order to ensure meaningful
quantitative
information, starting with the recording of the experimental data,
transforming
recorded information into digital form, correcting for anomalies of the
recording media and processing of the data. Procedures for obtaining
quantitative measurements
of important parameters such as chemical composition, grain boundary
expansion/contraction, strain and lattice parameters will be outlined,
including a
discussion of the accuracy of the measurements.
The workshop will cover problems associated with matching of calculated images

to experimental data and address the possibility of creating standards for
reporting
goodness of fit between experimental and simulated images and confidence
levels for
refined structural models. There will be one day devoted to reconstruction
methods
in HRTEM including a discussion on the limits of current reconstruction
methods.

Registration for the meeting is $150. Total number of participants is limited
to 40.
Application for registration can be obtained by contacting Gretchen Hermes
(see
contact information below) or by mailing or faxing (to Gretchen Hermes) the
application
form obtainable from the web-site listed below.

Gretchen Hermes
tel: 510-486-5006
fax: 510-485-5888
email: GHermes-at-lbl.gov

Total number of participants is limited to 40.

For information on registration and further details on the workshop, please
see the
web site:
http://ncem.lbl.gov/NCEM/workshops.html
--------------------------------------------------
Roar Kilaas
NCEM/MSD, MS 72-207
Lawrence Berkeley National Laboratory
1 Cyclotron Road,
Berkeley, CA 94720
tel: 510-486-4618
fax: 510-486-5888
email: r_kilaas-at-lbl.gov






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Thu, 7 Mar 1996 21:14:44 -0800
Subject: Re: Publisher of S. Klosevych Series?

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} Does anyone know who published, and the current source, of a series of
} tutorial papers called "Microscopy and Photomicrography"? They were
} written by Stanley Klosevych of the University of Ottawa.
Dear David,
The Stanley Klosevych series was published in the Microscopical Society of
Canada Bulletin. The Bulletin Editor, Caroline Emerson (email:
cemerson-at-kean.ucs.mun.ca), would be the best place to try for copies. I
don't remember exactly when the series was published. There is also a very
good book by him: "Principles and Practice of Microscopy and Scientific
Photography", which can be ordered on the MSC home
page:http://gause.biology.ualberta.ca/craig/hp/MSC.SMC.Mic.Soc.hp. or you
can link from the MSA/Microscopy site.
Luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Margaret H Malay :      malay-at-csd.uwm.edu
Date: Thu, 7 Mar 1996 23:44:48 -0600 (CST)
Subject: Midwest Meeting

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Microscopy of Surfaces and Interfaces
Midwest Microscopy and Microanalysis Society Workshop
UWM Microscopy Open House

April 26, 1996

Please register by April 15, 1996
More information and online registration at
http://www.uwm.edu/Dept/LSS/meeting.html

PROGRAM
Golda Meir Library Conference Center
University of Wisconsin - Milwaukee

Opening Ceremonies
8:45 - 9:00

Morning Session

9:00 - 9:30
J. Mansfield
University of Michigan - Ann Arbor
"Materials Science and Biological Applications of Environmental Scanning
Electron Microscopy"

9:30-10:00
B. Tonner
University of Wisconsin - Milwaukee
"X-ray Microscopy for Chemical Analysis of Surfaces"

10:10 - 10:15 Coffee Break and Mounting of Posters
Sponsored by College of Letters and Sciences

10:15 - 10:45
T. Kelly
University of Wisconsin - Madison
"Three-Dimensional Atom Probe Microscopy"

10:45-11:15
J. Nogami
University of Wisconsin - Milwaukee
STM: "Studies of Metal Growth on Silicon Surfaces"


11:15-11:45
L. D. Marks
Northwestern University - Evanstone
Surface HREM: "Old techniques on Old Surfaces - Surprising New Results"

Open House Lunch

11:45 - 1:00
Lunch sponsored by Nissei Sangyo America and Hitachi Scientific Instruments.
Poster Session

1:00 - 1:45
Poster viewing and poster competition
Poster prizes are sponsored by the MMMS.

Afternoon Session

1:45-2:15
J. M. Gibson
University of Illinois - Urbana
TEM: "All you ever wanted to know about building your own expensive and
complicated microscope, and then waiting five years until it finally works"

2:15 - 2:45
S. Babcock
University of Wisconsin - Madison
TEM/HREM: "Electron Microscopy Studies of Grain Boundaries in Bicrystals"

2:45 - 3:15
V. Dravid
Northwestern University - Evanstone
"Analytical Electron Microscopy and Holography of Interfaces: Making a
Mountain out of a Mole Hill"

3:15 - 3:30 Coffee Break and Judging of Posters
Sponsored by Laboratory for Surface Studies

3:30 - 4:00
N. Browning
University of Illinois at Chicago
"High Angle Annular Dark Field in a Dedicated STEM"

4:00 - 4:30
K.L. Merkle
Argonne National Laboratory
"Relaxation Modes in Metal and Oxide Grain Boundaries"

4:30- 4:45
Poster Awards Ceremony and Closing Address
M. Gajdardziska-Josifovska, Workshop Organizer

Open House Tour

REGISTRATION FEES:
Registration is free for the members of the Midwest Microscopy and
Microanalysis Society. The workshop registration fees for non-members are
same as the membership fees and can be paid at the conference site (by check
or cash):
Regular Member ($10)
Student Member ($5)

POSTERS
All lectures are by invitation only. Poster contributions by students,
researchers and microscope manufacturers are welcome. There are no size
restrictions for the posters, however, they should be mounted on a sturdy
backing suitable for display on easels. The poster must be relevant for the
broad topic of microscopy of surfaces and interfaces.

POSTER COMPETITION

Graduate students are particularly encouraged to present their work and
they are eligible to enter a poster competition. One grand prize and two
first prizes will be given by the Midwest Microscopy and Microanalysis
Society. The grand prize must be used by the winner to attend a microscopy
related session at a major national/international conference.

Grand Prize: $500
1st Biological Sciences:$100
1st Physical Sciences: $100








From: Tony Bruton :      bruton-at-emu.unp.ac.za
Date: Fri, 08 Mar 1996 11:01:54 +0200
Subject: Re: microscope disposal -Reply

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Message-Id: {s14013be.065-at-smtp.unp.ac.za}
X-Mailer: Novell GroupWise 4.1

We have just disposed of an old microscope - a HITACHI HU-11E.

After advertising it on the Net and finding no takers we dismantled it. We
kept things like DP and RP pumps, transformers, motors and guages but
found the recovery of scrap metal very limited. The chassis fetched the
princely sum of R33.00 from the local scrap dealer. Perhaps as a
teaching facility the greatest value was the fact that we now have a
wonderful assortment of dissected lenses, aperture holders, electron
gun etc to use as teaching material.

I don't believe that it is a kindness to donate a 27 year old fragile
valve-powered instrument such as this to a school or suchlike, the
maintenance responsibilities outside of expensive, specialised hands
are overwhelming.

An enquiry to the Japanese suppliers gave us a one-liner which we
have already framed - "Please throw away TEM as scrap metals"

Sobering moment seeing the frame of our beloved ex-TEM swinging
from a crane in the scrapyard before being dumped onto a pile of old oil
drums........

Tony Bruton
Centre for Electron Microscopy
University of Natal
Pietermaritzburg
KwaZulu Natal
South Africa





From: Wharton Sinkler :      sinkler-at-dvibm3.gkss.de
Date: Fri, 8 Mar 1996 11:32:46 +0000 (MET)
Subject: alignment of CM30

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Hello all:

I am using a Philips CM30ST for high resolution imaging. I have had a
problem performing the critical objective lens direct alignment "current
rotation center". Ideally, one should obtain a stationary point on the
specimen, rotating about its center at high magnification with a
low-frequency wobble applied to the objective lens current. I can get
the stationary point on the specimen reasonable satisfactorily.
The probjem is that the beam moves fairly significantly when the objective
lens wobbler is on.

I have performed the coma free direct alignment, which should ensure that
the beam is travelling along the optic axis of the objective lens. This
works well. However, it seems to me that if wobbling the objective lens
current can shift the beam, it can't be travelling the optic axis.

Is there any relatively quick way to correct the problem of beam shift
with objective lens current change? I can imagine that a complete
realignment of the microscope might get rid of the problem. However,
this is both very time-consuming and also not very instructive as to the
cause of the problem.

Wharton Sinkler

==================================================

Dr. Wharton Sinkler
GKSS Forschungszentrum Geesthacht
Abteilung WA
D-21502 Geesthacht
Germany

tel: (49)(4152)872542
fax: (49)(4152)872534




From: Ian MacLaren :      MACLARIZ-at-novell2.bham.ac.uk
Date: 8 Mar 1996 10:35:25
Subject: Re: TEM: preparation of ceramic fibres

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To: Microscopy-at-Sparc5.Microscopy.Com

Dear all,
I have received a whole bunch of suggestions about the above subject and
someone has asked me if I would share a summary of the responses with the
whole list, this is given below. Thanks again to all who responded. We
have decided to embed the fibres in epoxy as suggested by some of the
contributors, dimple and ion mill (at a low angle). I don't think that we
have a tripod polisher but I will be checking that out.

____________________________________________________________________________
My original message:

Dear all,
Has anyone out there prepared non-conducting ceramic fibres for TEM, either
across or along axis? If so, how did you do it?
We have some mullite fibres we would like to look at and although we have
some ideas about how to prepare specimens we would appreciate any ideas or
feedback on this topic.
____________________________________________________________________________
We have been routinely preparing ceramics fiber tows (Nextel, Nicalon, HPZ,
etc.) with and without ceramic coatings for TEM characterization for about
two years now. After much trial and error with a number of methods (some in
the literature) we have settled on a rather efficient method of producing
high-quality sections. We are just finishing a short communication on this
technique, which I could send to you when completed.

First, the tows are vacuum-impregnated with a high-temperature epoxy, such
as G-1. It is important that the fiber-volume fraction be high, which can
be achieved by either pulling many tows through the tip of a pipette, or
alternatively using shrink tubing. The epoxy is then allowed to cure.

The key to obtaining a good thin section with a large electron transparent
area is to minimize ion-milling time, which requires mechanically thinning
the sample to thicknesses less than 5 um, ideally less than 1 um. We use a
tripod polisher with 3M diamond lapping films to minimize differential
polishing and surface relief. The epoxy-impregnated tows have been thinned
both axially and transversely; either way wil produce good specimens.
Again, you want to thin the specimen as much as possible prior to ion
milling; if the specimen is too thick the epoxy will be milled away before
the fibers and any coating becomes electron transparent, with the fibers
usually falling out. We also need the epoxy to bound the coating for
coating thickness determination.

The specimen is then mounted on a Cu washer, and maybe a grid, and ion
milled at low angles for 30-60 min.

For more details please contact me off line.

Dr. Michael Cinibulk cinibumk-at-ml.wpafb.af.mil
Wright Laboratory/MLLM
Wright-Patterson Air Force Base, Ohio
____________________________________________________________________________
Do you have a tripod polisher in your prep lab.

We have a way of prepping fibers using a tripod that worked nicely
for cross and longitudinal sections of SiC fibers.

Ron Anderson
____________________________________________________________________________
I assume that you are aware of the difficulties in doing this. I know of
two methods which work very well and are based on other, relatively
well-known techniques for producing cross-sections.

A) embed in resin and ultramicrotome cross-sections; this is only
convenient if you have someone who does ultramicrotomy already, in which
case it is probably the simplest.

B) sandwich with epoxy between two silicon wafers and then cross-section
'normally,' just like a VLSI device; by visually monitoring optical
transmission through the silicon, you can thin the composite structure well
below 10 micrometers before ion-milling (which in turn helps mitigate
differential milling effects). This works for almost everything. The same
basic technique could be used to thin the fiber along the axis.

Please let me know if something easier comes along, since I have to be
doing this as well...

Daniel L. Callahan
Assistant Professor of Materials Science
Rice University
http://www.owlnet.rice.edu:80/~dlc/
____________________________________________________________________________
I had pretty good luck preparing ceramic fibers (mullite,
SiC, Al2O3/ZrO, and others), of 10 to 30 micron diameter,
for TEM analysis. The method is very simple, and consists
of first making a composite out of the fibers and an epoxy,
then thining down as with a bulk sample (polishing,
dimpling, ion milling). Things to keep in mind:

1. If the fibers are short and tangled as a small cotton
ball, saturate the epoxy with the fibers mixing very well.
The fibers will brake but that doesn't matter since is
unlikely that you will be interested in crystalline defects.
Squeeze the composite between two teflon or other
non-sticking sheets using glass slides for backings.


If the fibers are long (more than 3mm) you can lay down
a thin layer of epoxy on a teflon sheet, and the fibers on
top, one at a time or in bundles, in a single direction to
cover a strip 3mm wide x length of the fibers. Then squeeze
between two glass slides and another teflon sheet covered
with epoxy. Cure the epoxy as per manufacturer instructions.
In both cases you will be able to form a very thin composite
that requires a little grinding and polishing on both
sides before punching out 3mm discs, which can be further
polish to 100 micron. Dimple as usual (with diamond paste)

2. Epoxy: try with whatever you have available. Some
people prefer conductive epoxies. Initially, I used several
conductive and non conductive products from TRA-CON
(Medford, MA, 508-391-5550), but for some time now I have
settled on G1 epoxy from GATAN (412-776-5260).

3. Ion Milling: the mullite I worked with milled very fast
probably because it was very porous. Other ceramics mill
very slowly compared to the epoxy. This is a problem that
can be alleviated using a LN2 stage in the ion mill, to slow
down the milling rate of the epoxy, and having as much
fiber content as possible in the mixture.

4. Even if you use a conductive epoxy you may have to
carbon coat the thinned samples.

Good Luck!

Augusto Morrone
Univ. of Florida
Materials Science and Engineering
P.O.Box 116400
(352) 392-6985
amorr-at-mse.ufl.edu
____________________________________________________________________________
been using the Tripod polisher and a unique technique for tightly binding
fibers together. They have been working with SiC fibers, but I think that it
would work for you. Their Email addresses are
CINIBUMK-at-ml.wpafb.af.mil and SCHELTFJ-at-ml.wpafb.af.mil

- -Scott Walck
____________________________________________________________________________
I think that someone here used a Gatan PIMS to do this several years ago
with some success. The length of the fiber went across the 3mm washer.
John Hunt
____________________________________________________________________________
Over the years I've prepared various fibre materials in various ways. These
all should give results; the quality varies, but then so does the effort
required.

1) If the fibres are thin to begin with, pop them onto a support film
covered
grid, carbon coat, and voila!

2) If you have experience with ultramicrotomy, embed and section small
areas.

You may require a coupling agent to promote resin adhesion, and the result
of sectioning will be a mass of shattered fragments (unless the fibres are
amorphous), but then you might also get a huge amount of viewable area free
of chemical contamination.

3) Mix the fibres into a particularly hard resin such as Petropoxy which you
can then prepare (cut, grind, dimple, ion mill or tripod) as a monolithic
lump. I'm not aware of any resin hard enough to thin at the same rate as a
ceramic, but I've prepared SiC fibres this way with success.

4) Create a composite by electroless deposition of metal (usually Ni) which
you can then prepare in the ion mill. This one gives by far the best result
in my experience, but is also the trickiest and most potentially time
consumming. A short electroless plate to make the fibres conductive ,
followed by electrolytic deposition in standard plating baths is a good
option if you have access to such. The method I've used was adapted from
metal powder prep. techniques.

Let me know if you'd like further information on any of this.

###############################################################
# #
# Don Steele STEELE-at-KRDC.INT.ALCAN.CA #
# ALCAN INTERNATIONAL #
# Kingston Research and Development Center #
# P. O. Box 8400 #
# Kingston, Ontario Canada K7L 5L9 #
# #
###############################################################
____________________________________________________________________________
The answer is simple (at least in principle!) - embed them in a hard
epoxy resin or acrylic, then section them in an ultramicrotome,
collect on fine-meshed grids, pop in the TEM, and ---presto, nice
uniformly thin fiber cross-sections held together by the resin.
There will undoubtedly be some tearing of the section and/or
individual cross-sections. The fibers will be randomly dispersed so
that you'll get many orientations (inlcuding some near-longitudinal
slices, if they're useful). Be careful of beam effects - most
embedding materials are somewhat to terribly beam sensitive.

Sound too easy to be true? You're right, ultramicrotomy is somewhat
of an art, but can accomplish wonders in materials science. A good
reference is an overview by yours truly:

T.F. Malis and D. Steele, Specimen Preparation for Transmission Electron
Microscopy of Materials, MRS vol 199, Materials Research Society (1990) p.3.

If you don't have any MRS Proceedings there, I have a few copies left and
can send one, but only if you're seriously interested, as they're in short
supply. The people at UMIST in the Corrosion Center are experts in
this area as well. I tend to act as a information bank for this technique,
so let me know how you fare - I'm always looking for new references to
quote.

Tom Malis
Group Leader - Materials Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
Ottawa, Ontario
ph.: 613-992-2310
FAX: 613-992-2310
e-mail: tom.malis-at-cc2smtp.emr.ca
____________________________________________________________________________
This may or may not be applicable, but with the high-voltage EM
(lower interaction cross-section) and very low beam currents, we have been
able to image non- or poorly-conducting specimens without charging or heat-
ing problems. If this holds true for your instrument (e.g. you have an
IVEM), the advantage is that there may be no preparation required; the
disadvantage is that with these low doses you will either need a very sen-
sitive recording medium--LoDose or other x-ray film, image plates or in-
tensified or slow-scan CCD--or long exposures. The large grain or speci-
men drift could make the images useless. Good luck.
Yours,
Bill Tivol
____________________________________________________________________________
I am a little late in responding to your inquiry on ceramic fibers; My
apologies. Since some of the other responses mentioned using the wedge
technique for preparing thin TEM sections, I wanted to mention that BUEHLER
KKB, with whom I am affiliated, is a supplier of a wedge polishing tool such
as those mentioned. I previously was Applications Engineer for South Bay
Technology who offered the first commercially available tripod based
polisher. However, I have since come to work for BUEHLER, LTD in the US,
and we have made some changes to the IBM design in order to enhance the ease
of producing wedge samples. We also offer a complete line of diamond
lapping films for this polishing system.

If you would be interested in more information regarding BUEHLER's
MICROPRECISE(TM) Tripoint Polisher, I would be happy to have literature sent
to you, and/or have a salesperson contact you. If you would like more
information, please feel free to contact me directly by phone, fax or
e-mail.

Best regards,
Scott D. Holt
BUEHLER, LTD.
41 Waukegan Rd.
Lake Bluff, IL 60044 USA
Phone: (847)295-4546
Fax: (847)295-7942
102467.2752-at-compuserve.com
____________________________________________________________________________


Ian MacLaren,
IRC in Materials for High Telephone: 0121 414 3447
Performance Applications, FAX: 0121 414 3441
The University of Birmingham, email: I.MacLaren-at-bham.ac.uk
Birmingham B15 2TT,
England




From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Fri, 8 Mar 1996 08:03:36 -0500
Subject: S. Klosevych Series. Thank you.

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Eight is enough. I'd like to thank everyone who responded with the
reference information on this series. You can stop responding now.

David Rothbard

--
Institute of Paper Science and Technology






From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Fri, 8 Mar 1996 06:41:37 -0600
Subject: Re: alignment of CM30

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Coma-free alignment (stationary image of amorphous carbon when applying
plus/minus tilts at high magnification, 500-700K) and current centering
(specimen stationary when oscillating the lens current) are completely
different. The reason is that the top and bottom parts of the objective
lens are never completely lined up, the mechanical tolerances are too
severe. Coma-free alignment finds the optic axis in a true sense
from the sample downwards taking into account the misalignment of the
two parts of the objective lens. The beam is still likely to shift
when the objective lens current is oscillated since this is largely
determined by the top part of the objective lens. As a test, you
can measure the difference in tilt between:
a) Coma-free alignment
b) Current-center alignment
c) HV-center (oscillating the voltage, equivalent to testing the
alignment of all the post specimen lenses)
d) The tilt such that the beam does not translate when the objective
lens current is oscillated.

By comparing d) and a) you can get a measure of the misalignment between
the top and bottom parts of the polepiece; comparing a), b) and c) you can
check if the other post specimen lenses are well aligned. If the difference
between d) and a) is large then you have a bad lens, and you will have
problems getting small probes for microanalysis without artifacts (e.g.
tails).




From: randy nessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Fri, 8 Mar 1996 10:11:28 -0600 (CST)
Subject: Re: EDS Detectors for FE-SEM

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On Thu, 7 Mar 1996 MelanieOwl-at-aol.com wrote:

} Is it necessary to get a 30 mm window for an EDS detector to be used on a
} Schottky emitter FE-SEM in order to get enough counts, or is a 10 mm okay?
}
} Thanks in advance,
} Melanie Behrens
} Texaco, Inc.
} Fuels and Lubes Research Dept.
} Beacon, NY
} behrema -at- Texaco.com or MelanieOwl -at- aol.com

Our 10mm does fine, as long as the apts and lens are set properly.

Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu





From: bsgphy3-at-uconnvm.uconn.edu (JIM ROMANOW)
Date: Fri, 8 Mar 1996 11:29:49 -0500
Subject: Re: Microscope disposal

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} an old TEM?? I see many for sale, etc., but have never seen replies. We
} have an old Hitachi H-500 STEM, maintained by Hitachi until last year when
} the $ ran dry. It's been used strictly as a biological TEM for the last 7
} years or so. I would hardly thnk it would pay anyone to have it
} disassembled and shipped, and it can't be left in place. Thanks in advance
} for any input.

Do you have an art department? Some sculpture students are inspired by old
machine parts and they usually handle the shipping quickly. About 3 trips
for a Volkswagon bus (They were rated as half ton vehicles).

Good Luck
Jim

Jim Romanow
Electron Microscopy Facility
Physiology and Neurobiogy Department
The University Of Connecticut
Storrs
bsgphy3-at-uconnvm.uconn.edu






From: gins-at-acs.bu.edu
Date: Fri, 8 Mar 1996 14:00:47 -0500
Subject: recycling TEM's

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Alternate-Recipient: allowed
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On the subject, a friend uses the old vacuum pump from a Phillips for the
vacuum oven he uses for embedding in parafin and epoxy. I don't know
if it is qualitatively better thant a typical house vacuum line, but our
house vacuum has been known to be unreliable.

C.Ginsburg
Boston Univ. Med. School




From: GANTZ-at-med-biophd.bu.edu
Date: Fri, 08 Mar 1996 13:42:25 -0500 (EST)
Subject: Bench-top Photographic Processors

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Dear Doug:
We've had our Mohr Pro8 for about 9 months and love it. This model came
highly recommended by other users in the Boston area who had used them
much longer than us. We now wonder how we put up with the tray method
for so long! If you want more info, E-mail or call me.
Don Gantz
Boston Univ School of Medicine
gantz-at-med-biophd.bu.edu
617-638-4017

PS. AGFA also makes one but have no details.




From: Joe D Geller :      geller-at-world.std.com
Date: Fri, 8 Mar 1996 16:06:29 -0500 (EST)
Subject: Objective lens for Leitz Ergolux

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We are in need of a low power, 4X, objective for the Leitz Ergolux
optical microscope. I believe this is an infinity corrected optical
system. Might one be available?

Joe Geller
Geller MicroAnalytical Laboratory
426e Boston St.
Topsfield, Ma 01983-1212
508 887-7000 fax:887-6671




From: epicier-at-univ-lyon1.fr (Thierry Epicier)
Date: Mon, 11 Mar 1996 17:51:11 +0100
Subject: SIMPLY-S for HREM simulations on PCs

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Message-Id: {199603081842.MAA20224-at-Spacestar.COM}

The set of softwares SIMPLY-S, including for the multislice-based programs
SHRLI, and dedicated to various TEM/HRTEM calculations/simulations on
PCs (in the field of Materials Sciences) has recently been updated.
SIMPLY-S is available from ftp.univ-lyon1.fr (PUB/DOS/HRTEM : README.TXT
and SIMPLY1-2-3.ZIP files ; SIMPLY3.ZIP contains the updated version, while
SIMPLY1-2.ZIP files do correspond to the previous version, to be abandoned
soon).

Any microscopist interested can download the freeware version. remind also that
SIMPLY-S is permanently updated (check the date in the readme.txt file).

Comments and remarks are welcome !


______________________________________________

Thierry EPICIER
GEMPPM-502, INSA de Lyon,
69621 VILLEURBANNE, France
tel : (33) 72 43 84 94 (83 85)
FAX : (33) 72 43 85 28
Email : EPICIER-at-CISMSUN.UNIV-LYON1.FR





From: mallamaci-at-goodyear.com (Michael Mallamaci)
Date: Mon, 11 Mar 1996 12:55:03 -0500
Subject: Missed the ISO Guide 25 Summary

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Folks,

I seem to have missed the summary of the ISO Guide 25 Experience thread.
Was it ever out there? If somebody could forward it to me I would
appreciate the effort.

Thanks in advance,

Mike

Michael P. Mallamaci
The Goodyear Tire & Rubber Company
Analytical Sciences D/415A
142 Goodyear Blvd.
Akron, OH 44305 USA
--------------------------------
mallamaci-at-goodyear.com
(330) 796-7436






From: hawkey-at-neuro.duke.edu (Larry Hawkey)
Date: Mon, 11 Mar 1996 14:48:10 -0500
Subject: Has anyone processed E-coli for TEM?

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Some one wants to look at E-Coli in the EM. I have never worked with
bacteria before. Where could I find some good references on how to fix and
process them. Is there a standard fix or pH or anything I should be aware
of before I dive in.

any guidance would be most helpful.

Larry Hawkey
hawkey-at-neuro.duke.edu






From: dshubito-at-d.imap.itd.umich.edu (Dennis Shubitowski)
Date: Mon, 11 Mar 1996 14:05:50 -0500
Subject: Re: ZIP100 MAC/PC disk exchange easy if...

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(snip)

} We have not seen a utility to mount Mac formatted disks on DOS systems.
}
} Iomapple
} Iomega Technical Support

(snip)

I hold in my hand Conversions Plus from DataViz that converts files between
Mac and PC programs, including floppy disks, removable cartridges (SCSI) and
CR ROMS. I personally do not use it, but the good doctor in the office next
door uses it quite regularly as he runs a Windows NT server for both Macs
and PCs. The programs runs about $100 (US), but its smaller cousin MacOpener
allows you to use Mac floppies, removable media, and CD ROMS, but not to
convert. This runs about $50 (US).

The above programs apparently have done all they claim to do without a hitch,
whereas he had had problems with Mac-In-Dos, a program that claims similiar
capabilities.

Dennis Shubitowski
University of Michigan
School of Dentistry

(No connection to DataViz. I'm a Mac person!)






From: albrite-at-leonardo.net (Larry Albright)
Date: Sun, 10 Mar 1996 14:31:53 -0800
Subject: Members and Speakers wanted.

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Los Angeles Microscopical Society (LAMS)

LAMS has an ongoing need for interesting speakers for our monthly meetings.=
Topics could relate to the technique, history, applications, subjects or =
art involving microscopes. We are open to anything that is interesting,=
even if remotely related. This could be a good way to practice a new=
speech or presentation before a friendly and interested group.
LAMS is a non-profit organization founded in 1938, dedicated to the study=
and practice of the science, art, and history of microscopy and related=
topics. Membership covers individuals with an active interest and=
experience in optical and electron microscopy and associated science and=
technology. LAMS has a membership of around 100 people, and an average=
attendance of 40. The club has a monthly newsletter with a column by the=
President Gil Melle, usually on different aspects in the history of the=
microscope with artful illustrations of classic designs. We have=
corresponding members all around the globe. The membership fee is $25.00=
per year which includes the monthly bulletin. To join call Dave Hirsch at 3=
10-397-8357.


At this time, we conduct our meetings at the Edison Complex 1721 22nd Street=
in Santa Monica California. The meetings are held every third Wednesday=
of the month. Members gather about 7:00 p.m. and the program starting at=
8:00 p.m. The program usually lasts an hour and a half. There is a short=
refreshment break after the program and the second activity ending at 11:00=
.

The meeting room has a wall screen, blackboard,Carousel projector, large=
projection video, and other items available. Please let us know your needs.

Please e-mail to the Program Chair, Larry Albright {albrite-at-leonardo.net} =
or call if you are able to speak to our group, or if you would like to=
simply attend a meeting. The meetings are open to anyone.=20


Larry Albright
Program Chair
(310-399-0865)
albrite-at-leonardo.net=20






From: BGIAMMARA-at-magnum.mco.edu
Date: Mon, 11 Mar 1996 16:57:01 -0500 (EST)
Subject: MT1 Parts

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Hi you-all. I have come across a OLD Porter-Blum MT1 Ultramicrotome,
but are missing collets, knife holders, & specimen trimming block. Anybody
out there have any hearts to donate spare parts to make this thing sing?
A copy of the operating instructions book would be helpful, too.
Thanks a lot,

Beverly Giammara




From: James Lausier :      yojimbo-at-itsa.ucsf.edu
Date: Mon, 11 Mar 1996 15:03:47 -0800 (PST)
Subject: unsubscribe

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Please unsubscribe. Thank You




From: Ginger Baker :      lizard-at-okway.okstate.edu
Date: Fri, 8 Mar 1996 14:36:32 -0600
Subject: The Oklahoma Microscopy Society (OMS) Workshop in April

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On Friday, April 12, 1996 the annual Spring Workshop of the Oklahoma
Microscopy Society (OMS) will be held in Room 246 in the Noble
Research Center at Oklahoma State University (OSU), Stillwater,
Oklahoma.



The main focus of the workshop is "Digital Imaging and Image
Processing" and is being presented by Dr. David Bright, 1996 MSA
Speaker and a Research Chemist from the National Institute of
Standards and Technology in Gaithersburg, MD. The workshop will
include some of the following topics:

Image Processing for Microscopists: Practice and Pitfalls Image
Processing for the Macintosh: (free software available)
What are digital images and why would one want to process them?
Introduction to NIH Image and MacLispix
Introduction to Image Processing
-aspect ratio- "correction" for tilt or non square pixels -background
subtraction and normalization
-direction of gradient
-euclidian distance map
-mathematical morphology
-linear hough transform



Other items of business:

(1) Student Best Electron Micrograph Contest: Don't forget to
bring those prize-winning pictures with you to the workshop. Electron
micrographs will be judged by attendees at the workshop. The winner
will receive a $50.00 award and the micrograph will appear on the
cover of the Fall 1996 OMS Newsletter. Second place winner will
receive an award of $20.00.

(2) The Fall 1996 Technical Meeting in conjunction with the
Oklahoma Academy of Science will be discussed. This year it will be
held at the Oklahoma State University-OKC on Friday, November 8, 1996.
Abstracts are due Monday, September 16, 1996.

(3) Final call for the submission of micrographs suitable for the
new OMS t-shirt. Bring your entries to the spring workshop or mail
them to Ginger Baker, Sec. before that date. This contest is not
limited to students. The winner will receive a first edition of this
T-shirt absolutely free. Remember that the T-shirts should be
considered part of OMS' outreach efforts: they will be seen mostly by
the non-microscopist public. In the now-famous words of our OMS
president, "No hexamethylchickenwire lattice structures, please!".

(4) Discussion on the OMS web page expertly compiled and edited by
Dr. Scott Russell, University of Oklahoma.

Address: http://www.uoknor.edu/electron/oms/

From this site, you can travel to a variety of pages for up-to-date
microscopy news and information!

(5) Various meetings and shortcourses coming up during the year.
Also, updates on the gradeschool microscopy kits and their use in the
elementary classrooms.



Preregistration for the OMS Spring Workshop is $5.00 for members,
$10.00 for student non-members, and $15.00 for non-members. After
April 10 add a $5.00 late fee. On site registration begins at 8:30
a.m. Lunch is included in the fee.



Lunch: smoked turkey and brisket with barbecue sauce, cole slaw, baked
beans, relish, wheat/white bread, and peach cobbler.



Parking: Lot 96: Each person driving to the workshop must have a
visitor parking permit to hang in the vehicle with the designated lot
number.

For more information contact either:

Wanda L. Edwards Ginger R. Baker
President-Elect, 95-96 Secretary/Treasurer, 94-96
Email: kmk285-at-vms.ucc.okstate.edu Email: lizard-at-okway.okstate.edu
Phone: (405) 744-7415 Phone: (405) 744-6765
FAX: (405) 744-5275 FAX: (405) 744-8263





From: Larry D. Ackerman :      mishot-at-itsa.ucsf.edu
Date: Mon, 11 Mar 1996 16:09:37 -0800
Subject: photographic processors

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I have used an Agfa system, chemistry and paper for 12 years. The processor
was not sufficiently robust nor reliable for us. In any case it is no longer
available. The Agfa Variable Contrast Premium paper is my favorite emulsion
for scientific work. I recommend that you consider the Durst Printo system.
It is modular so you can build it to suit your own needs. It is my choice of
currently available systems.





From: Hand-at-nso1.uchc.edu (Hand,Arthur)
Date: Mon, 11 Mar 1996 09:29:05 -0500
Subject: TEM and SEM - Facility User Fees

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I would appreciate any information on current user fees at university EM
facilities. My institution has requested a comparison of our fees with those
of other facilities. Thanks for your help.

Arthur R. Hand, D.D.S.
Director, Central EM Facility
Univ. of Connecticut Health Center
MC 1610
Farmington, CT 06030
Tel: 860-679-4085
Fax: 860-679-4078
e-mail:
hand-at-nos1.uchc.edu





From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Mon, 11 Mar 1996 08:46:01 -0500
Subject: ZIP100 MAC/PC disk exchange easy if...

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Hi, I found some useful information on ZIP DRIVES FOR MIXED ENVIRONMENT.

The recent discussion on ZIP installation problems were not very
encouraging but most problems may have had their cause in inadequate
drivers and software.

Iomega's ZIP drives (not involved in any way with the company) are very
useful for image transfer between Mac and PC, especially 12-16 bit images
which do not fit on a floppy, session files, scanned images, image
processing derivatives from image files etc., as well as image exchange if
FTP is not available.

The following technical details come from AOL/Iomega Forum/Message
Board/Topic: ZIP Technical for PC/Mac. Also, all newest Iomega drivers for
Mac, DOS, Win can be copied from this site. I hope it helps. Best regards
Klaus.

********************************************************************************

} Is there any way for my Mac/Zip disks to be accessed on a PC /Zip drive or
} visa versa?

}
}
} Bernoulli, Zip, and Jaz DOS formatted disks can be mounted on a Macintosh.
} The disk needs to be Preformatted or formatted on a PC using a current
} version of either our Win95 or DOS/Win 3.1 utilities (Iomega Tools for
} Win95, Iomega SCSI or Zip Tools). The Macintosh needs to be running our
} driver version 3.5.3 for Bernoulli drives, version 4.2 or 4.3 for the Zip
} drive, or version 4.3 for the Jaz drive.
}
} The Macintosh needs to have a DOS mounting utility. The commonly found
} utilities are:
}
} 1. Apples' PC Exchange version 2.0.2 or greater. It is part of the Apple
} System 7.5 upgrade. This version of PC Exchange can be used with any
} version of System 7 and is available as a standalone product.
} 2. Insignia Solutions Access PC version 3.0 or higher.
} 3. Dayna Communications DOS Mounter Plus version 4.0 or higher.
}
} Each of these software packages will allow the Macintosh to mount DOS
} formatted disks. Consult with your local software vendor for information
} on which package is best suited for your needs.
}
} The Macintosh hierarchal file system (HFS) is not compatible with the FAT
} (DOS style) file system. Because of this we recommend the following
} precautions:
}
} 1. Create the directory structure on a PC and only copy files to the
} disk. Do not create or delete folders on the disk with the Macintosh.
}
} 2. Have backup copies of any file you are transfering. These files should
} be in their orginal format.
}
} 3. Disks that transfer files regularly should be formatted in a PC at
} regular intervals. Once every six months should be sufficient. The more
} often the disk is written to on the Macintosh, the more often this should
} be.
}
} Important Support Note:
} Each of the software packages mentioned should mount Iomega disks, just
} like they would mount a DOS formatted floppy disk. If any usability,
} installation, or compatibility problems are encountered contact the
} software vendor for technical assistance. Iomega does not provide
} technical support for these non-Iomega products.
}
} Problems we have seen:
}
} 1. You insert a DOS formatted disk into the drive and the Mac wants to
} initialize it.
}
} a. You can get this message if another driver is controlling the drive.
} Insert a Mac formatted disk into the drive and then Get Info about the
} disk itself. The line that says Where will tell you what driver is in
} control of the drive. At the end of the line you will see (3.5.3) for
} Bernoulli drives, (4.2) or (4.3) for Zip drives, and (4.3) for Jaz
} drives. Anything else would be conflict.
}
} b. The disk was formatted with Windows NT or a non-standard DOS format.
}
} 2. You try copying a singe large file to the disk and get the message
} "There isn't enough room on the disk."
}
} a. The DOS mounting utility has enough overhead in converting the file
} to limit the size of the file you can copy to the disk. It is roughly
} two-thirds the capacity of the disk.
}
} 3. After copying over about 500 files, the files seem to become corrupt.
}
} a. PC Exchange has a limit of the number of files it can copy just like
} DOS has a limit of the number of files that can be in the root directory.
}
} We have not seen a utility to mount Mac formatted disks on DOS systems.
}
} Iomapple
} Iomega Technical Support

} } } } } } } } } } } }
}
} WHAT ZIP DRIVE MODELS ARE AVAILABLE?
}
} A Zip SCSI model and a Zip Parallel Port model. The SCSI model can be
} used with Macintosh or a PC that is using a ZIP compatible SCSI adapter.
} The SCSI model has two 25 pin SCSI ports (for chaining), a SCSI ID
} switch, and a SCSI termination switch. The Parallel Port model can only
} be used with a PC and connects to the computers Parallel Port.
}
} WHICH SCSI ADAPTERS CAN BE USED WITH THE SCSI ZIP MODEL?
}
} Iomega provides a optional low cost SCSI adapter called the Zip Zoom
} Accelerator. Other ASPI compatible SCSI adapters can be used in
} conjunction with Iomega SCSI driver software.
}
} } } } } } } } } } } }
} Formatting a DOS ZIPs on the Mac (w/ PC exchange installed)?
}
} Yes there is a way of doing this. Go into the Tools program and click on
} Erase Disk. On the next window, select the long, ten minute format. Start
} the format and then stop it after five seconds and quit Tools. This will
} pop the disk out. Reinsert the disk and PC Exchange should take over and
} ask how you would like it formatted.
}
} We have seen problems doing it this way. Sometimes the newly formatted DOS
} disk isn't readable. We suggest that the formatting be done on a DOS
} system.
}
} Iomapple
} Iomega Technical Support

****************************************************************************
******

I hope these clarifications will help using the ZIP diks for image
transfers. For future reference, I have included this info in my web
page. Thanks Klaus



******************************************************************************
* : *
* Klaus-Ruediger Peters, Ph.D. : WWW Home Page: *
* Director, Molecular Imaging Laboratgory : *
* Biomolecular Structure Analysis Center : Molecular Imaging Laboratory *
* University of Connecticut Health Center : http://panda.uchc.edu/ *
* 263 Farmington Ave. : htklaus/index.html *
* Farmington, CT 06030-2017; U.S.A : Differential Hysteresis *
* : Processing Demo at http:// *
* Tel: (203) 679-3977; Fax: (203) 679-1989 : panda.uchc.edu./htbit/indiv/ *
* e-mail: Peters-at-BSAC.UCHC.EDU : /software_docs/dhp.html *
* : *
****************************************************************************
**






From: Owen P. Mills :      opmills-at-mtu.edu
Date: Sun, 10 Mar 1996 13:30:32 -0500
Subject: Need phone number in U.K.

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Hello,

I'm trying to contact the manufacturer of a power supply in our Oxford color
monitor, made in the U.K. Could someone in the U.K. help me by providing an
address & phone number for Cotron Electronics Ltd.?

Thanks.
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Sun, 10 Mar 1996 15:16:20 +0000 (GMT)
Subject: Re: alignment of CM30+other problem

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Tony,

The complete alignment of the CM30 takes about 1 hour, so if you happen
to have a computer connected to the microscope (allowing you to save and
load alignments data) it might be worth trying the alignment.

By the way I have another question about light variation. On our CM30,
the position of the light varies in a notable way depending on the C2
condensor excitation. If the C2 current is set at maximum, then back to
cross-over we do not get the light at the same place than if we set C2
current at minimum and then back to cross over. (at X 20000 the light
gets nearly out of the screen. I wrote to Philips, they told me that the
microscope was "within specification". I would have liked a more elaborated
answer, or even new inputs from CM microscopes users.

Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Luis Sole i Sabaris
E-08028 BARCELONA

Tel +34 3 402 16 95
Fax +34 3 402 13 98

On Fri, 8 Mar 1996, Wharton Sinkler wrote:

}
} Hello all:
}
} I am using a Philips CM30ST for high resolution imaging. I have had a
} problem performing the critical objective lens direct alignment "current
} rotation center". Ideally, one should obtain a stationary point on the
} specimen, rotating about its center at high magnification with a
} low-frequency wobble applied to the objective lens current. I can get
} the stationary point on the specimen reasonable satisfactorily.
} The probjem is that the beam moves fairly significantly when the objective
} lens wobbler is on.
}
} I have performed the coma free direct alignment, which should ensure that
} the beam is travelling along the optic axis of the objective lens. This
} works well. However, it seems to me that if wobbling the objective lens
} current can shift the beam, it can't be travelling the optic axis.
}
} Is there any relatively quick way to correct the problem of beam shift
} with objective lens current change? I can imagine that a complete
} realignment of the microscope might get rid of the problem. However,
} this is both very time-consuming and also not very instructive as to the
} cause of the problem.
}
} Wharton Sinkler
}
} ==================================================
}
} Dr. Wharton Sinkler
} GKSS Forschungszentrum Geesthacht
} Abteilung WA
} D-21502 Geesthacht
} Germany
}
} tel: (49)(4152)872542
} fax: (49)(4152)872534
}




From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Sat, 9 Mar 1996 11:39:23 -0600
Subject: Re: microscope disposal -Reply

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Message-Id: {v02120d02ad677209c345-at-[128.174.23.225]}
Mime-Version: 1.0
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Another idea: if you know someone with access to a decent machine
shop, and is skilled, you can have the scope column sawn in half
longitudinally to expose the parts _in situ_. We have a couple of TEMs
treated in this way on display, and they are useful teaching tools. (This
was done many years ago by an engineer who used to work here.)

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: Kukin V.N. :      lemi-at-mx.iki.rssi.ru
Date: Sat, 9 Mar 1996 13:00:52 +0300 (MSK)
Subject: Need program

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Message-ID: {199603091507.KAA28839-at-IndyNet.indy.net}
To: Microscopy list {microscopy-at-Sparc5.Microscopy.Com} , SPM List {spm-at-di.com}


I am trying to find any program that will allow to calculate
the isolated islands area and their size distribution using digi-
tal TEM images of metal or metal oxide thin films.

Thanks for any help that may be offered.
Vladimir Kukin,
Electron Microscopy Lab,
Moscow Institute of Electronic Technology (Technical University)
lemi-at-mx.iki.rssi.ru




From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Sat, 9 Mar 1996 09:44:46 +0000 (GMT)
Subject: Re: Lehigh address

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In order to get information aboput the excellent SEM and XRMA Short
Courses being offered by Lehigh University contact Ms. ShAron Coe by
e-mail at slc6-at-lehigh.EDU Sharon is a prompt e-mail replyer. See you in
June

Patrick Echlin
University of Cambridge UKOn Wed, 6 Mar 1996, MARK DARUS (216) 266-2895
wrote:

} Could someone send me information, or an E-Mail contact, for
} Lehigh (spelling may be wrong), in PA, so I can get information
} on courses offered.
}
} Thanks
}
} Darus-at-cle.dnet.ge.com
}




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Fri, 8 Mar 1996 13:38:39 -0500
Subject: Core Facility Position Available

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Message-Id: {v01520d00ad662e957006-at-[128.206.15.189]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

POSITION AVAILABLE: Associate Director, Molecular Cytology Core Facility
(confocal scanning laser microscopy, low light video microscopy, image
processing/analysis, microtomy, immunocytochemistry and in situ
hybridization). Individual with experience in some or all of these areas
is sought to train users, maintain instruments, develop protocols for a
multiuser facility. PhD desirable but not required for individuals with
extensive experience. Women and minority candidates are especially
encouraged to apply. Address applications (CV and 3 letters of reference)
or inquires to:
Thomas E. Phillips, Ph.D.
Division of Biological Sciences
3 Tucker Hall, University of Missouri
Columbia, MO 65211
573-882-4712
tphillips-at-biosci.mbp.missouri.edu.








From: dshubito-at-d.imap.itd.umich.edu (Dennis Shubitowski)
Date: Fri, 8 Mar 1996 16:43:24 -0500
Subject: Summary: Polaroid Film

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Message-Id: {v02120d01ad66581f3765-at-[141.211.157.94]}
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Content-Type: text/plain; charset="us-ascii"

Many thanks to all who responded about my Polaroid film dilemna. The emulsion
ended up washing right off in an overnight soak. Hopefully this does not
happen again. If it does, however, I am armed with an arsenal of tips!


Thanks again,

Dennis Shubitowski
University of Michigan
School of Dentistry


Here is a summary of the responses:

I have never been successful in separating T55 negatives that have dried
together.

John
chandler-at-lamar.ColoState.EDU

-----

I did SEM for 7 years at Wayne State and ran into that problem at least several
times. Let me tell you straight: it can't be done and isn't worth the effort.
You'll always get some junk to remain on the negative ruining it forever. Oh, I
hope the negatives aren't too important!

Walt Bobrowski
Parke-Davis Research

-----

Have you tried a little detergent in the water? The liquid type is the
best to use.

I don't know if this is a sure thing, I always used a water soak, but my
success may be due to the local water.

Hope this helps.

Ellie Solit
The Microscope Book

-----

I have used a AGFA AGEPON-WATER SOAK on TEM nagatives before
That worked

Stephan H Coetee
Electron Microscope Unit
Private Bag 3
Wits
2050 Stephan-at-Gecko.biol.WITS.ac.za

-----

Yes, I have successfully unstuck pairs of Polaroid negs. Actually, the
water soak will work, but you have to soak them for a long time, at least
overnight, say 12-16 hours. Then you have to slowwwwwwwly pull them apart to
avoid pulling the emulsion off. A "pucker" mark may remain where they were
joined, but usually its translucent so that a positive print will seldom reveal
the pucker mark, especially on "busy" image detail, and as long as original
image density was not disturbed.

When this happens to my negs, its usually in a spot no larger than the size of a
quarter (25 cent piece). If its most of the area of the negs, that is serious
trouble.

When I dry Polaroid negs in racks, I skip slots so that there is lots of space
between adjacent negs to prevent sticking from occuring as the negs "wiggle" as
they air dry.

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

-----

I have sometimes (!) been successful by giving the negatives a prolonged
soak (days) in water. the problem, of course, is that the emulsion can be
washed off - you have to hope that the negatives unstick before you lose the
emulsion. Good luck!

Tony Garratt-Reed

-----

I remember a water soak working for me, but not without waiting over a
weekend. Even then it left a patch ...

{\/} /\ {\/} /\ {\/} /\ {\/} cognito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/}
Michael Shaffer, R.A. - University of Oregon Electron Probe Facility
mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu
http://darkwing.uoregon.edu/~mshaf/epmahome/

-----

I have had limited success with an overnight Photoflo soak
followed by ultrasonication. Films stuck emulsion to film
back seem to separate easier than those stuck emulsion to
emulsion.

richard.mount-at-mailhub.sickkids.on.ca

-----

I agree with Richard, above, that film back stuck to emulsion is easier to
seperate, and this is the case I usually run into as I dry my Polaroid negs in
racks with the emulsion of one facing the back of the next neg.

A point that I left out in my previous note describing successful use of
overnight water soak to seperate stuck negs is how I set this up. As with many
techniques, success lies in the details of the method. I load a pair of stuck
negs into my usual neg drying rack with edges of one neg in one set of slots,
edges of other neg in adjacent set of slots. The effect is to separate the negs
where they are not stuck to enable water to get directly to the stuck area.
Success depends on getting good water soak and softening of the film emulsion.
If you were to simple lay the two stuck negs flat in a pan of water, might not
get as good a soak as when the films are seperated a bit to allow water access.

I hope this helps.

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

-----

YES, virginia, polaroid negatives can usually be unstuck, just soak in
very hot water about 10 -20 minutes, then GENTLY pull them apart, GRADUALLY
turning and pulling a little untilthe whole gelatine that is stuck gets
soaked through and losened. Some times if more completely dried out, the
emulsion of one will be stuck to the second, usually the second can be saved
completely, but some of the first will be lost (natually the center, best
part of the image)
good luck

Alan S. Pooley ,PhD Bivalve shell SEM & shape analysis
SEM/Morphometrics lab
Marine & Coastal Sciences
Rutgers University
908 932 8959 ext 225
Pooley-at-ahab.rutgers.edu







From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Fri, 8 Mar 1996 20:14:46 -0800
Subject: Re: Microsc.Soc Canada Web page

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} Dear Mary, I read your notice about Stan klosevych's papers in the MSC
} bulletin,
} and tried to locate the NSC home page on the web address you gave. My computer
} running under Netscape did not recognise this. i would still like to know more
} of Stan's articles, as i teach microscopy. Can you help. TIA Jeremy Sanderson.
Dear Jeremy,
As several of my friends informed me, the Web site I gave for the MSC had a
small typo that made it unusable. In my defense, I copied it correctly from
the current MSC Bulletin. They had it wrong. The correct address is:
http://gause.biology.ualberta.ca/craig.hp/MSC.SMC/Mic.Soc.hp
As I said, there is also a link from the MSA WWW site.
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Rudi Rottenfusser :      rrotte-at-mbl.edu
Date: Fri, 8 Mar 1996 12:00:42 -0500
Subject: Zeiss Parts

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Message-Id: {199603081701.MAA04205-at-hoh.mbl.edu}
Sender: {rrotte-at-mbl.edu}
microscopy-at-Sparc5.Microscopy.Com
Priority: normal
X-mailer: Pegasus Mail/Windows (v1.22)

Good morning.

For your Universal M microscope equipped with the "Vertical
Illuminator IIC", you will need a 22mm diameter glass plate (semi-
transparent mirror). You can install this into your "H-PL" housing.

It is still available as a "spare part" from Zeiss under catalog
number 466260-0010 at U.S. $72.00. For ordering spare parts,
please call
U.S.: 800/233-2343
Canada: 800/387-8037

The other option you always have is to call one of the companies
which manufacture all kinds of filters such as

Chroma Technologies (Vermont): Tel. 802/257-1800
Omega Optical (Vermont) Tel. 802/254-2690.

Please e-mail me if I can be of further help.



You wrote " I have a Zeiss Universal 'M' microscope which is missing a few
} parts, which are now VERY (?) expensive (from Zeiss).
}
} I require a REFLECTOR for a 'Vertical Illuminator IIC'; either a
} H-PL, or H-PR will do. Only the reflector is required as I have the
} housing. Does anyone have one of these ? Or does anyone know if it is
} possible to 'make' one using a 'double sided semi-transparent mirror' ?
} ==============================================================================
} Steve Schwarz sschwarz-at-morgan.ucs.mun.ca
} Dept. of Earth Sciences
} Memorial University of Newfoundland
} Newfoundland
} CANADA
} A1B 3X5
} 1-709-737-8142
} -737-2589 FAX





From: Walter Arno Mannheimer :      wamann-at-metalmat.ufrj.br
Date: Fri, 8 Mar 1996 14:09:22 EST3EDT
Subject: disposal of old microscopes

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We decomissioned our Stereoscan Mk II after 25 years of service, as
an instrument with open (graduate) student access. Managing the
electronic tubes just became impossible.
The Engineering School mantains a Museum, were we gave it honourable
burial. But first we took out pumps, oscilloscope, camera, some
gauges, etc. which continue to serve usefully. The empty shell still looks nice,
and who would know the difference.
It is now among scale models of locomotives, our first computer, etc.
This instrument was installed in 1969. We know it was the first SEM in
Brazil, probably also in South America, and we like to think it might
have been the first in the Southern Hemisphere. Anyone like to
comment on this?
Prof.Walter A.Mannheimer
Metallurgy and Materials Engineering
Federal University of Rio de Janeiro
POBox 68505
21945 Rio de Janeiro Brazil
Voice 5521 280-7443 (Dept.office) 5521 590-0579 (direct)
Fax 5521 290-6626 Email: wamann-at-metalmat.ufrj.br




From: Theresa A. Fassel :      fassel-at-post.its.mcw.edu
Date: Fri, 8 Mar 1996 17:00:05 -0600 (CST)
Subject: fungi samples

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Hi

I will receive Candida albicans samples in my lab for service. I am
looking for a good preservation of cell wall, cell membranes, nuclei, mitos,
ribosomes, etc. I must keep the cell wall intact for this study. ALso,
I want to avoid acrolein.

Does anyone have a solution ?? ANy advise?


Thanks in advance

Theresa

Dr. Theresa A. Fassel
Sr. Research Associate fassel-at-post.its.mcw.edu
Department of Microbiology (414)-456-8410
Medical College of Wisconsin Fax (414)-266-8522
8701 Watertown Plank Road
Milwaukee, WI 53226-0509






From: lmiller-at-ux1.cso.uiuc.edu (Lou Ann Miller)
Date: Sat, 9 Mar 1996 08:51:29 -0500
Subject: Re: fungi samples

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Message-Id: {v01510100ad67357c7777-at-[128.174.23.58]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hi Teresa,



I have done Yeast, lichen, soybean and tobacco seedlings with microwave
fixation and processing, and have been quite pleased with the vast
improvement in results.

I have used both a standard microwave and a nice one from Ted Pella
specifically for EM.

There are many ways to do this, but the following is what we follow in our lab:

Microwave rules:
1. Always use at least a 250 ml water load to collect the heat
and keep it off your sample.
I change this every 16 seconds of use ( 2 fixations). ****
Always set the water load in
the same place each and every time.

2. Calibrate the Microwave. Set the water load/ beaker in
it's designated spot.
Use liquid crystal sheets and microwave about 15 seconds.
-- Observe where the hot spots show up, and avoid them.
Pick a place preferable
inbetween hot spots.

3. Always do a pilot study first.

4. Things to avoid like the plague:

--- Always chill your sample in wetted down crushed ice for
at least 30-60 seconds
before microwaving. This will allow MW, but keep heat off
your sample.
**** As soon as you are finished microwaving , put back
into the ice for a moment.

---- Always wipe off the outside of your vial, for water
draws heat ( hence the
water load situations) Also never allow water to be
anywhere but in the
waterload beaker.

--- Using the same principle, only use enough fixative/
solution to just cover the
sample. I usually use about 1-2 mm high. Greater volumes
will EXPONENTIALY
(sp) increase the heat load to your sample. The difference
between 6 drops and 1 ml
can be 40C!!!!!

Processing times:
==============


You have to experiment with this.

I'd start out with possibly the following for things with thick walls and
for plants:

1. Ice sample, wipe, microwave for 7 seconds, ice.

2. Repeat the above for a total of 3 times.

3. Rinse in buffer.

4. Put into OsO4---2%aq.

Ice, MW 7 seconds, let set 10 min................change solution
and repeat once more.
And save the last OsO4 on the sample.

****** it is amazing how MW speeds up processing times, for the
first time ever after
starting microwave, I have to really watch OVER staining enbloc.
So...... repeat a 3rd time if you don't like the staining of the
first, but try doing only 2
OsO4's at first.

5. To the last OsO4 solution on sample, Add equal volume of 3%
Potassium Ferricynide.
Set for 20 minutes only.

6. Rinse with water.

7. Add filtered saturated aq Uranyl Acetate, no older than 10 days.
( Might be our water problem)

Ice, MW 7 seconds Ice, and let set for 15 minutes at room temperature.

REPEAT

8. Dehydrate as norm. 8-10 min. each step.

9. Infiltration: -- these will get warm, it's OK, and even helps greatly.


1:1 MW 10 seconds, no ice, incubate (rotating)15 minutes and REPEAT

1:4 MW 10 seconds, no ice, incubate 1 hour and repeat.

Pure epoxy: MW 10 seconds, no ice Incubate 1 hour intervals and
overnight.
Repeat MW in am, make at least 2 changes of
epoxy.

** Also helps to spend overnight in vacuum in a
desicator hooked up to
house vacuum.

The difference I've seen in structure, staining and non-pitting out of the
specimens vs the more traditional methods I've used is incredible. We like
microwave so much we now use it for all of our samples.

I'm going to be leaving for a week, but if you'd like to know more, I can
get you references etc when I'm at work when I get back. Our methods are
based on Logan's, Ted Pella's and my 2 years of working through techniques.


Good Luck,

Lou Ann



} Hi
}
} I will receive Candida albicans samples in my lab for service. I am
} looking for a good preservation of cell wall, cell membranes, nuclei, mitos,
} ribosomes, etc. I must keep the cell wall intact for this study. ALso,
} I want to avoid acrolein.
}
} Does anyone have a solution ?? ANy advise?
}
}
} Thanks in advance
}
} Theresa
}
} Dr. Theresa A. Fassel
} Sr. Research Associate fassel-at-post.its.mcw.edu
} Department of Microbiology (414)-456-8410
} Medical College of Wisconsin Fax (414)-266-8522
} 8701 Watertown Plank Road
} Milwaukee, WI 53226-0509

***************************
Lou Ann Miller
Microscopic Imaging Lab
College of Vet. Medicine
University of Illinois
2001 S Lincoln Ave
Urbana,Illinois 61801
217-244-1566
lmiller-at-ux1.cso.uiuc.edu

Microscopy Home Page:
http://www.cvm.uiuc.edu/MicImagLab/MicImagLab.html

Personal Home Page:
http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/LAM.html






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 11 Mar 1996 17:11:33 -0400
Subject: RE-Origin of C Particles

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Message-ID: {n1385558009.21007-at-mse.engin.umich.edu}

Subject: Time: 5:05 PM
OFFICE MEMO RE:Origin of C Particles Date: 3/11/96

It may well be that by carrying out a proper analysis of your materials
using the various technidques of optical microscopy (polarized light,
refractive index meas, etc.), you can get info about the source of the carbon
particles. The people at McCrone Associates Inc (850 Pasquinelli Dr,
Westmont, IL 60559 (Ph: 708-887-7100, Fx: 708-887-7417) are world reknown
specialists in identifying particulate materials and determining their
sources. I suggest you get in contact with them.
W. C. Bigelow (bigelow-at-umich.edu)





From: Johari, Dr. Om :      73211.647-at-CompuServe.COM
Date: 11 Mar 96 08:09:34 EST
Subject: Updated info on SPM program at Bethesda

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"Margoninski, Yohanan" {Ymargo-at-VMS.HUJI.ac.il} ,
"Markewicz, Peter" {pmarkiew-at-chem.utoronto.ca} ,
"van der Heide, Paul" {ldm-at-apollo.numis.nwu.edu} ,
"Marti, Othmer J." {Othmar.marti-at-physik.uni-ulm.de} ,
"Maugel, Tim" {maugel-at-zool.umd.edu} ,
Microscopy {Microscopy-at-aaem.amc.anl.gov} ,
"Mochizuki, Yasunori" {mochizuk-at-sci.cl.nec.co.jp} ,
"Molecular Imaging (Gatan)" {info-at-molec.com}

-- [ From: Dr. Om Johari * EMC.Ver #2.0 ] --
Expanded recipient data:
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To: DAVID JOY \ Internet: (djoy-at-utk.edu)
To: Keller, David \ Internet: (dkeller-at-triton.unm.edu)
To: Kavanagh, Karen \ Internet: (kkavanagh-at-ucsd.edu)
To: Kenny, Peter \ Internet: (p.g.kenny-at-bradford.ac.uk)
To: Kenny, Peter \ Internet: (p.g.kenny-at-bradford.ac.uk)
To: Kiyoshi Kobayashi \ Internet: (kiyoshi_kobayashi-at-vnet.ibm.com)
To: Bernd W. Koenig \ Internet: (bernd-at-helix.nih.gov)
To: Kotorman, Louis \ Internet: (kotorman-at-craft.camp.clarkson.edu)
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To: Lazebnik, Yuri \ Internet: (lazebnik-at-cshl.org)
To: Lea, Peter J. \ Internet: (spectral-at-inforamp.net)
To: Leapman, Richard D. \ Internet: (leapman-at-helix.nih.gov)
To: LEO (UK) \ Internet: (info-at-leo-em.co.uk)
To: Lewis, Nathan S. \ Internet: (nslewis-at-juliet.caltech.edu)
To: HaLi \ Internet: (hl-at-padns1.phy.tu-dresden.de)
To: Lieber, Charles M. \ Internet: (cml-at-cmliris.harvard.edu)
To: Lindsay, Stuart \ Internet: (stuart.lindsay-at-asu.edu)
To: Lyo, In-Whan \ Internet: (lyo-at-watson.ibm.com)
To: Marek Malecki \ Internet: (malecki-at-macc.wisc.edu)
To: Murphy, Judy \ Internet: (murphy-at-ms.sjdccd.cc.ca.us)
To: Nagy, Peter \ Internet: (p.nagy-at-r1.atki.kfki.hu)
To: Newbury, Dale E. \ Internet: (newbury-at-enh.nist.gov)
To: INTERNET:dmnewns-at-watson.ibm.com \ Internet: (dmnewns-at-watson.ibm.com)
To: Nicholov, Rina \ Internet: (nicholov-at-botany.utoronto.ca)
To: Jun Nogami \ Internet: (nogami-at-csd.uwm.edu)
To: Norde,Willem \ Internet: (willem-at-metten.fenk.wau.nl)
To: Peter J.A. Nordlander \ Internet: (nordland-at-kaitum.rice.edu)

please circulate/post

Please complete/return form below to remain/get on our lists.

Scanning Microscopy International
P.O. Box 66507, Chicago (A.M.F. O'Hare), IL 60666-0507, U.S.A.
Telephone: (708) 529-6677 / FAX: (708) 980-6698
E.mail: 73211.647-at-compuserve.com

Scanning Microscopy 1996 meeting
May 11-16, 1996, Bethesda, Maryland (suburb of Washington, DC)

Symposium on: Scanning Probe Microscopies and Related Techniques
for the Biological and Materials Sciences

Note: Nearly 35 SPM related papers will be presented during the
program on "Pattern Formation and Nanoscaled Structures in Thin
Film Formation" at the same time at same venue. THOSE PAPERS ARE
SEPARATELY LISTED BELOW.

Program organizers: Dr. David P. Allison, Oak Ridge Natl. Lab., TN
(E-mail: allisondp-at-ornl.gov); Prof. Chunli Bai, Chinese Acad. Sci.,
Beijing (E.mail: clbai-at-infoc3.icas.ac.cn); Prof. Masamichi
Fujihira, Univ. Yokohama, Japan (E.mail:
mfujihira-at-bio.titech.ac.jp); Dr.
Heinrich J.K. Hoerber, European Molec. Biol. Lab., Heidelberg,
Germany (E.mail: hoerber-at-embl-heidelberg.de); and Prof. Douglas J.
Thomson, Univ. Manitoba, Winnipeg, Canada (E.mail:
thomson-at-ee.umanitoba.ca).

VERY FEW papers can still be offered: please contact one of the
organizers or Dr. Om Johari at Scanning Microscopy International.

The registration fee for the entire 6-day conference (without
subscription to any of the SMI journals' 1996 volumes) is either
$100 (if payment reaches SMI before March 31, 1996) or $120 (from
April 1, 1996); the fees with 1996 subscription to one, two or all
three SMI journals (Scanning Microscopy, Cells and Materials, and
Food Structure) are respectively: $180, $220, or $270 (for US
delivery addresses), or $210, $270, or $340 (for outside US
addresses). The room rates (exclusive of applicable taxes,
currently 15%) at the Hyatt Regency Bethesda (One Bethesda Metro,
Bethesda, MD 20814, USA; phone: 301 657 1234; FAX 301 657 6453)
are: Single (1 person - 1 bed): $100; Double (2 persons - 1 bed)
or Twin (2 persons - 2 beds): $110. Please make room reservations
directly with the hotel.

List of presentations (as of March 10, 1996) in alphabetical
order (the name of the presenter is capitalized when there are more
than one authors)

M.J. ALLEN, Digital Instruments, Santa Barbara, CA; E.M. Bradbury,
R. Balhorn:
The Chromatin Structure of Well-Spread Demembranated Human Sperm
Nuclei Revealed by AFM

D.P. ALLISON, P.S. Kerper, M.J. Doktycz, T. Thundat, R.J. Warmack,
Oak Ridge National Lab., TN:
High Resolution Physical Mapping of EcoRI Restriction Sites on
Intact Cosmids by AFM Imaging

P.C. Zhang, C. BAI, P.K.H. Ho, Q. Li, Y. Dai, Y.S. Wu, B.F. Sheng,
Chinese Acad. Sci., Beijing, China:
AFM Study of Interactions Between Tumor Necrosis Factor and Its
Monoclonal Antibodies

A.A. BERGMAN, A.P. Quist, C.T. Reimann, S.O. Oscarsson, B.U.R.
Sundqvist, Uppsala Univ., Sweden:
Antibody-Antigen Docking Observed with Tapping-Mode Scanning Force
Microscopy in Air

A.A. BUKHARAEV, N.V. Berdunov, D.V. Ovchinnikov, Kazan Physical-
Technical Inst., Russia:
Three-Dimensional Probe and Surface Reconstruction for Atomic Force
Microscopy Using Deconvolution Algorithm

R.J. Pylkki, G.J. COLLINS, Topomatrix, Santa Clara, CA:

Scanning Thermal Microscopy with a Positive Probe

E.D. DAHLBERG, R. Proksch, S. Foss, G. Skidmore, C. Merton, Univ.
Minn, Minneapolis:
Review: Magnetic Force Microscopy and Magnetotactic Bacteria

P. DEWOLF, T. Trenkler, T. Clarysse, W. Vandervorst, IMEC, J.
Snauwaert, L. Hellemans, Leuven, Belgium:
Electrical Characterization of Submicrometer Silicon Devices by
Cross-Sectional Contact Mode AFM (to be presented in the
Semiconductor program)

S.J. EPPELL, R.E. Marchant, Case Western Reserve Univ., Cleveland,
OH:
Atomic Force Microscopy of Bone: More than an Image (to be
presented during the Bone Biology program)

R. ESCHRICH, Max-Planck-Inst. Biochem., Martinsried, Germany; G.L.
Kumar, T.A. Keil, R. Guckenberger:
AFM on the Olfactory Dendrites of Giant Silkmoth Antheraea

G.L. Kumar, Max-Planck-Inst. Verhaltenphysiol., Seewiesen, Germany;
R. ESCHRICH, R. Guckenberger, T.A. Keil:
In Search for Putative Pheromone Receptors on the Membrane of
Olfactory Dendrites in Silkmoths (A. polyphemus and A. pernyi)
Using the AFM and SEM

M. Fujihira, Tokyo Inst. Tech., Japan:
Review: AFM of Solid Surfaces in Aqueous Solutions

L.A. GHEBER, J. Hwang, M. Edidin, Johns Hopkins Univ., Baltimore,
MD:
Imaging of Rough Biological Samples in Liquid with Fluorescence
Near Field Scanning Optical Microscopy

D.T. GODDARD, A. Steele, I.B. Beech, British Nuclear Fuels,
Preston, UK:
AFM of Bacterial Biofilms with Application to Microbially
Influenced Corrosion

H. Hansma, Univ. Calif., Santa Barbara:
Review: Atomic Force Microscopy of Biomaterials

M. HARA, W. Knoll, RIKEN, Saitama, Japan:
Review: STM and AFM Studies of Self-Assembled Monolayer Growth

D.O. HENDERSON, Y.S. Tung, R. Mu, Fisk Univ., Nashville, TN; W.A.
Curby, M. Mercado, Fed. Aviation Rech. Ctr., Atlantic City, NJ:
Optical and Atomic Force Microscopy of Pentaerythritol Tetranitrate
Nanoclusters on Si(100)

C.L. Mosher, E. HENDERSON, Iowa State Univ., Ames:
Review: Chemical and Biomolecular Force Detection

R. Eckert, S. Jeney, W. Oeffner, J.K.H. HOERBER, European Molecular
Biology Lab., Heidelberg, Germany:
Measuring Surface Forces with the AFM

D.H. HUANG, Y. Yamamoto, Yamamoto Quantum Fluctuation Project,
Tokyo, Japan:
Hydrogen Atom Extraction and Redeposition on Hydrogen-Terminated
Silicon Surface with Ultra High Vacuum STM at Room Temperature

A. IKAI, Tokyo Inst. Tech., Japan, K. Mitsui, M. Hara:
Review: Measurements of Mechanical Parameters of Proteins and
Chromosomes

A. IKAI, H. Takeuchi, S. Kawauchi, Tokyo Inst. Technol., Japan
STM Study of Stearoyl Amide and Anilide

M.D. JOHNSON, Univ. Oklahoma, Norman, and H.W.M. Salemink:
Review: Cross-Sectional STM on Semiconductor Heterostructures (to
be presented during the Semiconductors program)

I. KARL, J. Bereiter-Hahn, Univ. Frankfurt, Germany:
Regulation of All Surface Motility Revealed by Scanning
Acoustic Microscopy

L. KUUTTI, J. Pere, J. Peltonen, O. Teleman, VTT Biotech. Food
Res., Espoo, Finland:
Interpretation of High Resolution AFM Images of Crystalline
Cellulose Using Molecular Modelling

R.M. LeBlanc, Univ. Miami, Coral Gables, FL:
Review: Scanning Probe Microscopies of Langmuir-Blodgett Films

G. LEE, A.D. MacKerell, L.A. Chrisey, R.J. Colton, US Naval Res.
Lab., Washington, DC:
Measuring Inter- and Intramolecular Forces in Biomolecules

A.L. LITVIN et al., US Army Natick R&D Center, Natick, MA
AFM and Optical Studies of Organic Thin Films with Hydrogen-Bonded
Networks

L.S. Shlyakhtenko, S. Adhya, T. Aki, Y. LYUBCHENKO, Arizona State
Univ., Tempe:
AFM Studies of Regulatory Nucleoprotein Complexes

F.K. MANTE, Univ. Pennsylvania Dental School, Philadelphia, G.
Baran:
Nanoindentation Studies of Single Crystal Titanium

J.F. Marchiando, NIST, Gaithersburg, MD::
Methods for Interpreting Measurements from a Scanning Capacitance
Microscope (to be presented during the Semiconductors program)

M.T. MCDERMOTT, Univ. Alberta, Edmonton, Canada, J.-B.D. Green,
M.D. Porter:
Review: Chemical Mapping with Force Microscopy

M. Rivera, M. MILES, R.L. Williamson, Univ. Bristol, UK:
Phase Transitions in Liquid Crystals Observed by SPM

W. MIZUTANI, M. Motomatsu, H. Ogiso, H. Tokumoto, Nat. Inst. Adv.
Interdiscpl. Res., Tsukuba, Japan:
STM with Local Non-Linearity Detection of Organic Thin Films and
Ion Irradiated Graphite

D.J. MUELLER, F. Schabert, A. Engel, Univ. Basel, Switzerland:
Review: Structural Changes of Native Membrane Proteins Monitored at
Subnanometer Resolution with the AFM

F. MUELLER, A.-D. Mueller, Tech. Univ., Chemnitz, Germany:
Scanning Capitance Microscopy of Composite Materials

H. MURAMATSU, Seiko Instruments, Chiba, Japan; T. Ataka, N. Chiba,
K. Nakajima, M. Fujihira
Review: Fluorescence Imaging and Spectroscopy of Biomaterials in
Air and Liquid by SNOM/AFM

R. Perez, Univ. Cambridge, UK:
First Principles Simulations of Atomic Resolution in Non-Contact
AFM (to be presented during the Fundamental Physics Program)

R. RAITERI, Univ. Genoa, Italy, H.-J. Butt, Max-Planck-Inst.
Biophy., Frankfurt, Germany:
Changes in Surface Stress Measured with an AFM

B. SAMORI, A. Gordano, I. Muzzalupo, Univ. Bologna, Italy:
DNA Deposition on Mica for Scanning Force Microscopy Imaging

W. Haiss, J.K. SASS, Fritz-Haber-Inst., Berlin, Germany:
STM Surface Stress Measurements in Electrochemistry (tentative)

A.J. Simon, Merck Res. Lab., West Point, PA:
Review: Molecularly Combed DNA: First Stretched, Then Imaged

R.P. Singh, Indian Min. Sci. Tech., New Delhi:
STM and Application Possibilities

Y. Shirane, Univ. Tokushima, Japan:
Surface Observation of Calcium Oxalate Monohydrate Crystals by FE-
SEM and AFM (to be presented during the Stones and Crystals
program)

F. El Feninat, C. Poulin, T. Ellis, E. Sacher, I. STANGEL, McGill
Univ., Montreal, Canada:
The Applications and Limitations of AFM to Complex Biomaterials
Surfaces: Effects of Acid Demineralization on Human Dentin

M.S. UNLU, B.B. Goldberg, Boston Univ., MA:
Review: Characterization of Materials and Devices by Near Field
Optical Scanning Microscopy (to be presented during the
Semiconductors program)

J. Vesenka, Calif. State Univ., Fresno:
Review: The Diameter of Duplex and Quadruplex DNA Measured by SPM

VU THIEN BINH, F. Feschet, V. Semet, S.T. Purcell, Univ. Lyon,
France:
Fresnel Projection Microscopy: Theory and Experiment: Electron
Microscopy with Nanometer Resolution at ~ 200 eV

C. Lebreton, Z.Z. WANG, CNRS Lab. Microstr. Microelec., Bagneux,
France:
Pulse Width Dependence of Nanowriting

G. Brown, M.B. WEIMER, Texas A&M Univ., College Station, TX:
Adsorption and Interaction of Ammonia Molecules on GaAs(110)
Studied with STM

F. Zenhausern, IBM Watson Research Ctr., Yorktown Hts., NY:
New Developments in Near Field Optical Microscopy/Spectroscopy

---

Please circulate / Publicize

Scanning Microscopy International
Post Office Box 66507, Chicago (A.M.F. O'Hare), IL 60666-0507,
U.S.A.
Telephone: (708) 529-6677 / FAX: (708) 980-6698
e.mail: 73211.647-at-compuserve.com

Scanning Microscopy 1996 meeting; May 11-16, 1996, Bethesda,
Maryland

Program on:

Pattern Formation and Nanoscaled Structures in Thin Film Formation

Organized by: Prof. Martin Zinke-Allmang, Univ. Western Ontario,
London, Canada (E-mail: M.Zinke-at-uwo.ca; FAX 519 661 2033), Prof.
Hiroshi Iwasaki, Osaka, Japan (E-mail:
iwasaki-at-sanken.osaka-u.ac.jp; FAX: 81-6-8798404) and Prof. Ellen D.
Williams, Univ. Maryland, College Park (E-mail:
williams-at-surface.umd.edu; FAX: 301 314 9465).

Program Focus: (1) Surface and Interface Defect Formation and
Structure; (2) Phase Separation on Surfaces and Epitaxial Growth
Modes; (3) Surface Roughness, Step Formation, and Dynamics During
Growth; (4) Characterization Techniques with a Focus on Electron
Microscopy and Tunneling Methods; (5) Synthetic Sublithographic
Nanostructures; and (6) Defect Engineering and Related Applications
for Mismatched Materials.

A FEW Papers can still be offered, please contact one of the
organizers or Dr. Om Johari at Scanning Microscopy International.

List of presentations (as of March 11, 1996) in alphabetical order

P. Campbell, Naval Res. Lab., Washington DC:
Nanofabrication with Proximal Probes

G. Carlow, Univ. West. Ontario, London, Canada:
Hummock Formation and Evolution During Ion Bombardment of
Rotating Substrates

O. Enea, Univ. Poiters, France:
Topographic Studies of TiO2 and SnO2 Ceramics by Environmental
Scanning Electron Microscope, SEM and AFM

R. Feenstra, Carnegie Mellon Univ., Pittsburgh, PA:
STM Studies of Semiconductor Thin Film Structures

E.A. Fitzgerald, MIT, Cambridge, MA:
CVD Grown Si-based Semiconductor Film Structures

D. Gerthsen, Univ. Karlsruhe, Germany:
Atomic Scale Investigation of the Mismatch Relaxation in
In0.6Ga0.4As/GaAs(100)

M. Grant, McGill Univ., Montreal, Canada:
Theoretical Studies of the Dynamics of Nanostructure Formation

M. Hong, AT&T Bell Labs., Murray Hill, NJ:
Novel Heterostructures Fabricated Using In-Situ Molecular Beam
Epitaxy

J.W.P. HSU, Q. Xu, Univ. Virginia, Charlottesville, E.A.
Fitzgerald, Y.H. Xie, P.J. Silverman:
Scanning Probe Microscopy Studies of Electrically Active Defects
in Lattice Mismatched Films

R. Hull, Univ. Virginia, Charlottesville:
Real-Time Nanostructural Studies of Defects and Degradation Modes
in Semiconductor Materials and Devices

M. Ichikawa, JRCAT, Tsukuba, Japan:
Observation and Formation of Nanostructures Using a Combined
Microscope with Scanning Reflection and Scanning Interference
Electron Microscopes and STM

A. Iwamoto, T. Yoshinobu, H. IWASAKI, Osaka Univ., Japan:
Scanning Probe Microscopic Studies of Surface Kinetic Roughening

H.-C. JEONG, J.D. Weeks, Univ. Maryland, College Park:
The Dynamics of Steps on Vicinal Surfaces During
Reconstruction-Driven Faceting

H. KIRK, Z. Radzimski, G. Rozgonyi, North Carolina State Univ.,
Raleigh:
Electron Beam-Induced Current (EBIC) Characterization of Defects
in SiO2 and SiO2/Silicon Interface

A. Kowal, Polish Acad. Sci., Krakow, Poland:
Properties of Nickel Hydroxide Layer Formed Electrochemically on
the Surface of Polycrystalline Ni in 1M KOH Studied by In Situ
Atomic Force Microscopy

M. KUNITAKE, N. Batina, K. Itaya, JRDC/ERATO: Itaya
Electrochemistry Project, Sendai, Japan:
In Situ STM Study of Self-Organized Molecular Layers on an
Iodine-Modified Au(111) Surface

J.W. Lyding, Univ. Illinois, Urbana:
UHV-STM Nanofabrication and Semiconductor Interface
Characterization

S. MATLIS, H. Ron, I. Rubinstein, Weizmann Inst. Sci., Rehovot,
Israel:
AFM Study of Gold Oxide Stabilized with Self-Assembled Monolayer

K. Matsumoto, Electrotechnical Lab., Tsukuba, Japan:
Application of STM/AFM Nano-Oxidation Processes to Room
Temperature Operated Single Electron Transistors and Other Devices

J.J. McClelland, NIST, Gaithersburg, MD:
Nanofabrication via Laser Focusing of Atoms

I. Nevernov, Technobiochip, Marcina, Italy:
Sub-Micron Machining of Al Tracks Deposited onto a Silicon Wafer

R. Nishitani, T. Umeno, A. Kasuya, Y. Nishina, Kyushu Inst.
Technol., Iizuka, Japan:
Correlation Between STM-Induced Photon Mapping and the STM
Topography of Nanometer-Size Metal Particles

D.D. PEROVIC, B. Bahierathan, Univ. Toronto, Canada, D.C. Houghton,
H. Lafontaine, J.-M. Baribeau:
Morphological Instability Phase Diagrams: Mapping Thin Film
Strain Relaxation Behaviour

C. K. Shih, Univ. Texas, Austin:
Scanning Probe Microscopy and Spectroscopy of Semiconductor
Heterostructures

J. Stroscio, NIST, Gaithersburg, MD:
Iron and Chromium Growth Studies

I. TERESHKO, V. Khodyrev, E. Lipsky, Mechanical Engg. Inst.,
Mogilev, Belarus:
Self-Organizing Processes and Modification of Thin Films by
Scanning Microscopy

R. Tromp, IBM, Yorktown Hts., NY:
LEEM Related Studies and Optical Microscopy of Semiconductors

L. Whitman, Naval Res. Lab., Washington, DC:
The Atomic Scale Structure of Semiconductor Surfaces: What Makes
the Atoms Smart?

S.-L. YAU, Y.-G. Kim, K. Itaya, JRDC/ERATO: Itaya Electrochemistry
Project, Sendai, Japan:
Scanning Tunneling Microscopy of Chemisorbed Benzene on Rh(111)
and Pt(111) Electrodes in Hydrofluoric Acid Solution

J. ZEGENHAGEN, Max-Planck-Inst. Festkoerperforschung, Stuttgart,
Germany, P. Lyman, M.J. Bedzyk, M. Boehringer:
Review: Analysis of Microscopic Structure of Stress Induced
Reconstructions on Semiconductor Surfaces

M. ZINKE-ALLMANG, T.D. Lowes, Univ. Western Ontario, London,
Canada:
Cobalt Clustering on Si at Low Temperatures

---

Other related programs at the same venue (*: Fliers available):

*Fundamental Physics in Microscopy and Microanalysis (~ 25 papers),

*Scanning Microscopy and Semiconductors: Metrology and Diagnostics
(25+ papers);

Several Biological programs including: Microanalysis and Imaging
(12 papers),
Immunolabelling (8 papers), *Radiation Effects / Apoptosis (25+
papers), *Dentistry (20+ papers), Corrosion Casting (including a
program on Lung: 10 papers), Inner Ear (6 papers), *Bone Biology
(35 papers), *Stones and Crystals (35 papers);

Several Biomaterials Related Programs organized under "*Cells and
Materials": (1) Skeletal Tissue / Biomaterials; (2) Foreign Body
Reactions; (3) Biointerfacial Reactions at Biomaterial Surfaces;
(4) Innovative Drug Delivery Systems; (5) Blood-Related
Biomaterials; and (6) Dental Biomaterials. (nearly 70 papers)

----------------

For more information, please complete and return the form below to
SMI:

__ I wish to present at the Scanning Microscopy 1996 meeting
(tentative title and summary on a separate sheet), please send a
Letter of Intent form. IT IS VERY LATE NOW!

__ I cannot present, but am likely to attend the 1996 meeting,
please keep me informed and send me: ___ 1996 Registration / Hotel
form.

__ I can neither present nor attend; please add/keep my name on
your mailing list. ___ Send me a mailing list form.

Please send: 1996 program fliers (*list programs here):



___ Instructions for Authors / ___ Major subject index / Table
of Contents for: ___ Scanning Microscopy / ___ Cells and
Materials / ___ Food Structure;

Flier on Scanning Microscopy:

___ Supplement 6, 1992 ("Signal and Image Processing in
Microscopy and Microanalysis");

___ Supplement 7, 1993 ("Physics of Generation and Detection of
Signals Used for Microcharacterization");

___ Supplement 8, 1994 ("Science of Biological Microanalysis")

___ Flier on the special issue: Interface Formation and Dynamics in
Layered Structures (Scanning Microscopy, Vol. 8, no. 4, 1994).

___ Flier on 1996 Pfefferkorn Conference on Electron Image and
Signal Processing, May 18-22, 1996 at Silver Bay, New York


Name

Title

Organization / Company


Address

City/State

Postal Code

Country

Phone

FAX

E.mail

Date




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Mon, 11 Mar 1996 16:19:46 -0600
Subject: SEM: videotaping from SEM

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
Message-Id: {v02130502ad6a56342c43-at-[131.230.97.68]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

We have a Hitachi 2460N variable pressure SEM and wish to do videotaping.
The unit has a jack called "Video Out" that we use to obtain quick prints
from a video printer. When we attempted to video tape from this jack, we
obtained a poor quality image (dim, blurry, noisey, etc.). It appears that
this might work but that some intervening device (signal booster) may be
needed. Anyone have any experience with this? Many thanks.

#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Ginger Baker :      lizard-at-okway.okstate.edu
Date: Thu, 7 Mar 1996 17:02:48 -0600
Subject: OMS Spring Workshop, April 12, 1996, OSU, Stillwater, OK

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Mime-Version: 1.0

On Friday, April 12, 1996 the annual Spring Workshop of the Oklahoma
Microscopy Society (OMS) will be held in Room 246 in the Noble
Research Center at Oklahoma State University (OSU), Stillwater,
Oklahoma.



The main focus of the workshop is "Digital Imaging and Image
Processing" and is being presented by Dr. David Bright, 1996 MSA
Speaker and a Research Chemist from the National Institute of
Standards and Technology in Gaithersburg, MD. The workshop will
include some of the following topics:

Image Processing for Microscopists: Practice and Pitfalls
Image Processing for the Macintosh: (free software available)
What are digital images and why would one want to process them?
Introduction to NIH Image and MacLispix
Introduction to Image Processing
-aspect ratio- "correction" for tilt or non square pixels
-background subtraction and normalization
-direction of gradient
-euclidian distance map
-mathematical morphology
-linear hough transform

Other items of business:

(1) Student Best Electron Micrograph Contest: Don't forget to
bring those prize-winning pictures with you to the workshop. Electron
micrographs will be judged by attendees at the workshop. The winner
will receive a $50.00 award and the micrograph will appear on the
cover of the Fall 1996 OMS Newsletter. Second place winner will
receive an award of $20.00.

(2) The Fall 1996 Technical Meeting in conjunction with the
Oklahoma Academy of Science will be discussed. This year it will be
held at the Oklahoma State University-OKC on Friday, November 8, 1996.
Abstracts are due Monday, September 16, 1996.

(3) Final call for the submission of micrographs suitable for the
new OMS t-shirt. Bring your entries to the spring workshop or mail
them to Ginger Baker, Sec. before that date. This contest is not
limited to students. The winner will receive a first edition of this
T-shirt absolutely free. Remember that the T-shirts should be
considered part of OMS' outreach efforts: they will be seen mostly by
the non-microscopist public. In the now-famous words of our OMS
president, "No hexamethylchickenwire lattice structures, please!".

(4) Discussion on the OMS web page expertly compiled and edited by
Dr. Scott Russell, University of Oklahoma.

Address: http://www.uoknor.edu/electron/oms/

From this site, you can travel to a variety of pages for up-to-date
microscopy news and information!

(5) Various meetings and shortcourses coming up during the year.
Also, updates on the gradeschool microscopy kits and their use in the
elementary classrooms.



Preregistration for the OMS Spring Workshop is $5.00 for members,
$10.00 for student non-members, and $15.00 for non-members. After
April 10 add a $5.00 late fee. On site registration begins at 8:30
a.m. Lunch is included in the fee.



Lunch: smoked turkey and brisket with barbecue sauce, cole slaw, baked
beans, relish, wheat/white bread, and peach cobbler.



Parking: Lot 96: Each person driving to the workshop must have a
visitor parking permit to hang in the vehicle with the designated lot
number.

For more information contact either:

Wanda L. Edwards Ginger R. Baker
President-Elect, 95-96 Secretary/Treasurer, 94-96
Email: kmk285-at-vms.ucc.okstate.edu Email: lizard-at-okway.okstate.edu
Phone: (405) 744-7415 Phone: (405) 744-6765
FAX: (405) 744-5275 FAX: (405) 744-8263





From: TKWZ51A-at-PRODIGY.COM (MR ALEXANDER W GINGELL)
Date: Thu, 07 Mar 1996 17:40:13 EST
Subject: TN5500

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Alex W. Gingell * EMC.Ver #2.10P ] --

Thanks to those who replied to my request for used Tracor TN500 EDS
systems for sale.

Unfortunately all my e-mail from last week has been destroyed! Please
re-send.

Best regards,

Alex Gingell
Tha





From: tom von moltke :      tomm-at-iscorltd.co.za
Date: Tue, 12 Mar 1996 15:14:37 +0200
Subject: unsubcribe

Contents Retrieved from Microscopy Listserver Archives
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From: jjt-at-vet.purdue.edu (Dr. John Turek)
Date: Tue, 12 Mar 1996 08:09:04 -0500 (EST)
Subject: sectioning titanium alloy

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I have had a request from a materials science colleague to section a
titanium alloy. He wants a blockface of several square mm and a thickness
of 10 microns. Is this possible to do? Is there anyone out there who would
be willing to do this for a fee? Thanks.

John Turek
Purdue University
Dept. Basic Medical Sciences





From: klivi-at-jhu.edu (Kenneth JT Livi)
Date: Tue, 12 Mar 1996 10:09:29 -0400 (EDT)
Subject: TEM and SEM - Facility User Fees

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Arthur Hand wrote:

} I would appreciate any information on current user fees at university EM
} facilities. My institution has requested a comparison of our fees with those
} of other facilities. Thanks for your help.

At The Johns Hopkins Department of Earth & Planetary Sciences, we set the
fee 11 years ago for in-house use at $35/hr for either the TEM or the
microprobe. Add additional fees for operators (cost depending upon whether
operator is student, staff, or faculty). TEM film is included in hourly
rate, but polaroid film is not supplied for EMPA use and holey-C grids are
charged for TEM use. Time starts when filament is turned on. This
includes calibration time for the EMPA.

Cheers,

Kenneth JT Livi
Department of Earth and Planetary Sciences
34th and Charles Streets
The Johns Hopkins University
Baltimore, Maryland 21218
(410) 516-8342 (voice)
(410) 516-7933 (fax)
klivi-at-jhu.edu (e-mail)






From: jlong-at-electrosource.com (James Long)
Date: Mon, 11 Mar 1996 14:12:31 -0600
Subject: Re: photographic processors

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Alternate-Recipient: allowed
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Message-Id: {960312113205.628-at-cliff.ml.wpafb.af.mil.0}

At 10:12 AM 3/7/96 -0600, you wrote:
} The best processor out there is the Mohrpro. We have been using one
} for about 8 years and recommend it when ever we can. It is easy to
} use and maintain, 2 min. dry to dry, permanent (not stabilized) and
} uses RC paper and Kodak Fixer.

I must respectfully disagree. I was personally responsible for implementing
both an Ilford 2150 and a Mohr Pro 8" Model in a multiuser lab. While I
liked the Mohr and our users were mostly happy with it, from an ease of use
and maint. point of view, I would have to go with the Ilford.

The 2150 ran flawlessly for several years, then needed minor (in-house)
maintenance - float sensors went bad. It will process up to 20" wide sheet,
in 90 secs. dry-dry, and the rollers stay submerged in the chemistry, which
seems to keep them cleaner (no dried on chemicals to deal with).

The Mohr has some rollers partially exposed, which often was a source of
roller marks, and were more difficult to clean. We used it primarily for
negatives (TEM & SEM). It performed well, just not as smoothly as the Ilford.

Overall, I had very good support from both Ilford and Mohr (though neither
produced problems that really tested the waters). I would recommend both,
but for different apps - Ilford for RC Prints (does not do film), and the
Mohr for negs. With the caveat that the Mohr will require more hands-on time.

JCL
=========================================================
James C. Long
Manager/Materials Analysis Lab
Electrosource, Inc.
512-445-6606
jlong-at-electrosource.com





From: Ann-Fook Yang :      YANGA-at-em.agr.ca
Date: Tue, 12 Mar 1996 13:27:46 -0500
Subject: Cryo accessory for Ultracut E microtome

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Message-Id: {s1457b18.020-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

I am looking for a used set of cryo accesssory for Reichert
Ultracut E microtome. If anyone has one and willing to sell,
please contact me directly. Thank you.

Ann Fook Yang
e-mail: Yanga-at-em.agr.ca
Phone: 613-759-1638





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 12 Mar 1996 13:23:19 -0400
Subject: RE-Heater calibration

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Message-ID: {n1385485336.93602-at-mse.engin.umich.edu}

Subject: Time: 1:09 PM
OFFICE MEMO RE:Heater calibration Date: 3/12/96

We just went through the problem of trying to calibrate a heating device for
TEM specimens. In the process we made use of some very fine thermocouples
which we found can be purchased at a very reasonable price from the Cole
Parmer Company (7425 N. Oak Park Ave., Chicago, IL 60648; 800-323-4340).
These are bare wire thermocouples (which means they are very flexible and
easy to get into awkward places), and they are available in wire diameters
from 0.03" down to 0.005", and in several combinations of wires for different
temp ranges and applications.





From: Walter Arno Mannheimer :      wamann-at-metalmat.ufrj.br
Date: Tue, 12 Mar 1996 15:19:11 EST3EDT
Subject: ISO summary

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Microscopy members,
I have been in correspondence with Jordi Marti. He has been having
problems with email, and has asked me to broadcast that the ISO
summary (which was already posted) did not make it to the system. It
will be reposted as soon as problems are solved (a few days)
Prof.Walter A.Mannheimer
Metallurgy and Materials Engineering
Federal University of Rio de Janeiro
POBox 68505
21945 Rio de Janeiro Brazil
Voice 5521 280-7443 (Dept.office) 5521 590-0579 (direct)
Fax 5521 290-6626 Email: wamann-at-metalmat.ufrj.br




From: Bill Sgammato, NORAN Instruments, Inc.
Date: 12 Mar 96 15:54:41 EST
Subject: Subscribe

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Please add me to the mail list for Microscopy.
My E-Mail address is 103506,222-at-compuserve.com

Thank You,

Bill Sgammato





From: John Lafeber :      jlafeber-at-rust.net
Date: Tue, 12 Mar 1996 17:34:44 -0500 (EDT)
Subject: unsubcribe

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unsubcribe
***********************************************

John C. Lafeber, P.E.
4165 Stein Road
Ann Arbor, MI 48105

Phone: 313-761-8819
Fax: 313-761-9943
E-mail: jlafeber-at-fe3.rust.net
Web: http://www.rust.net/~jlafeber
FTP (Pick up file location) ftp.netcom.com/pub/jl/jlafeber

"People have vision, computers should too."





From: John Lafeber :      jlafeber-at-rust.net
Date: Tue, 12 Mar 1996 17:34:53 -0500 (EDT)
Subject: unsubscribe

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unsubscribe
***********************************************

John C. Lafeber, P.E.
4165 Stein Road
Ann Arbor, MI 48105

Phone: 313-761-8819
Fax: 313-761-9943
E-mail: jlafeber-at-fe3.rust.net
Web: http://www.rust.net/~jlafeber
FTP (Pick up file location) ftp.netcom.com/pub/jl/jlafeber

"People have vision, computers should too."





From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Wed, 13 Mar 1996 19:16:54 -0600
Subject: Test Message from Nestor

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Sorry for the traffice folks, but we had a problem
today on the Server. This is just a test message
so that I can try to locate the source.

Nestor
Your Friendly Neighborhood SysOp






From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Thu, 14 Mar 1996 08:49:45 +0000 (GMT)
Subject: Re: RE-Heater calibration

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Dear Wil:
I read your e-mail message about heater calibration with interest. You
might like to seek out a paper some colleagues and I wrote about 20 years ago
Scanning Electron Microscopy 1976 Part I pages 83-90 in which we describe
how we fabricated some very thin film thermocouples for use in the SEM.
The women who did the work was extraordinarily patient and very nimble
with her fingers. They worked, and showed us that a frozen sample kept at
ca. 150K did not heat up when irradiated with the electron beam.

Patrick Echlin
Cambridge




From: James S MArtin :      James.S.Martin-at-williams.edu
Date: Thu, 14 Mar 1996 09:12:12 -0500 (EST)
Subject: polaroid back for sale/trade

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Message-Id: {199603141323.IAA28742-at-hoh.mbl.edu}

We have an Olympus polaroid camera back that has never been out of the
box. The part numbers, descriptions and 1990 list prices are:

PM-DL-W adapter for large format back 436.00
PM-CP-W 3 1/4 x 4 1/4 in. Polaroid back 260.00

If you are interested in purchasing these items or trading for something
... please contact me directly.

James Martin




From: Jake Schaper :      Jake_Schaper-at-chdqm.sps.mot.com
Date: 14 Mar 1996 09:38:03 -0700
Subject: Subject-SEM Repair

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Message-Id: {n1385325847.24632-at-chdqm.sps.mot.com}

Can anyone refer me to a reliable independent SEM service company that can
repair an S200? I'm in the Phoenix area, so a company near here or the west
coast would be desirable.


**********************************************************
Jake Schaper
ASIC Product Analysis Lab
Motorola, Inc.
1300 N. Alma School Rd. Chandler, Arizona 85224
Mail Drop CH240
Phone 602-814-4756
**********************************************************






From: RNBALDUC-at-ARCRIDE.EDU.AR
Date: Thu, 14 Mar 1996 14:53 -0300
Subject: searching SEM

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Dear Microscopysts

We are searching an used SEM for received it in donation .The SEM will be
used for Research and Education in our School.
Please, we need know the model, year of fabrication, possibility of work,
and other information available.
We pay all the costs of shipmen.
Please, contact me for any questions.

E-mail : RNBALDUC-at-arcide.edu.ar

Address:

Fernando Balducci
Laboratory of Electron Microscopy
School of Bioengineering.
National University of Entre Rios.
C.C 57 Suc 3
Parana - Entre Rios
Argentina.





From: klivi-at-jhu.edu (Kenneth JT Livi)
Date: Thu, 14 Mar 1996 14:47:28 -0400 (EDT)
Subject: Phantom EDS low-energy tails

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Greetings to all listeners,

I have an interesting problem that I hope someone has some insight on. I
have a light element EDS detector attached to a Philips 420 Supertwin, and
processed pulses are analyzed by the DTSA/4Pi/Mac system. I have observed
an unusual phenomenon that is interfering with our ability to model the
shapes of characteristic peaks and backgrounds. All the peaks exhibit
low-energy tails (LETs) that come and go depending upon a few parameters.
They are:

(1) After a day of the detector bias being on but no X-rays enetering the
crystal, the first spectrum of that day will have large LETs (} 3% of total
peak area) that decrease with further exposure to X-rays.
(2) LETs can be reduced if the bias is turned off for some time, but they
will reappear after a day.
(3) Conditioning the crystal only effects LETs as would turning off the bias.
(4) Previous detectors have not shown as severe LET problems.
(5) The amount of LETs can be reduced by lowering accelerating keV.
(6) Every single component of this system has been replaced, including the
crystal (3 times), but excluding the Mac computer and the TEM. This has
had no effect.
(7) There appears to be no problems related to ground-loops.

Observations 1 through 4 suggest a problem with the current detector
system, but 5 and 6 may imply a problem with the scope alignment. Has
anyone experienced similar problems with their detector? I have heard
rumors that this has appeared on other TEMs, but is not a problem on SEMs
and EMPs.

Many thanks to anyone with ideas.

Kenneth JT Livi
Department of Earth and Planetary Sciences
34th and Charles Streets
The Johns Hopkins University
Baltimore, Maryland 21218
(410) 516-8342 (voice)
(410) 516-7933 (fax)
klivi-at-jhu.edu (e-mail)






From: krogers-at-ecn.purdue.edu (Kirk Rogers)
Date: Thu, 14 Mar 1996 16:24:04 -0500
Subject: WTB: X-Ray detector

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Microscopy Folks-

We would like to request that if someone has an old EDS unit that
will do qualitative analyses and hook up to a PGT detector, we'd be
interested in looking at it and possibly purchasing. We don't need fancy
bells, whistles, image analysis, dot-mapping ect. Just a basic unit that
will take an x-ray spectrum and do some simple analyses.

The names/contact info. of reliable used equipment vendors would also be of
assistance.

Thanks,



-Kirk
_____________________________________________
Kirk Rogers krogers-at-materials.ecn.purdue.edu
OR kirk.a.rogers.1-at-purdue.edu
Purdue University, School of Materials Science and Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289
OFFICE: 317-494-8751 FAX: 317-494-1204
http://materials.ecn.purdue.edu/~krogers






From: Daniel L. Callahan :      dlc-at-owlnet.rice.edu
Date: Thu, 14 Mar 1996 16:09:13 -0600 (CST)
Subject: Si transmission web-page

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Good Day:

I have placed a picture of a flat-wedge of optically transmitting (100)
silicon on a web page: the image has thickness scale bars from 0 to 10
micrometers. This isn't an ideal sample: for example, there are no
interference fringes visible in the submicron regime. However, it should
serve as a starting reference. Please let me know what you think. The
image is at

http://www.owlnet.rice.edu:80/~dlc/silicon.html

I am considering making a higher angle wedge hoping to preserve more of
the thin region and also taking an image under a Na lamp as advised by
Ron Anderson. Other suggestions are welcome as would other images,
particularly of other orientation.


Daniel L. Callahan
Department of Mechanical Engg. and Materials Science
Rice University
dlc-at-owlnet.rice.edu




From: GANTZ-at-med-biophd.bu.edu
Date: Thu, 14 Mar 1996 18:18:06 -0500 (EST)
Subject: Vitreous Ice Layer Thickness Determination

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Hi Everyone:
I'm working on some projects using vitreous ice cryomicroscopy and
would like to be able to determine the thickness of final ice layers on
holey films. In general, I've heard of a couple of methods: 1) Burn a
small hole, tilt, photograph; or 2) relative density by densitometry of
negatives. I would be appreciative to receive details and references
on these methods or other methods being used. What sort of accuracy
should one expect from the various methods? Thanks for your help.
Don Gantz
Boston Univ. Med School
e-Mail: gantz-at-med-biophd.bu.edu




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Thu, 14 Mar 1996 19:30:55 -0600
Subject: SEM Videotaping Problem Solved

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X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
Message-Id: {v02130505ad6e77c4714a-at-[131.230.97.68]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Thanks to all who sent helpful suggestions regarding our inability to
videotape from our S2460N variable pressure SEM. The problem was caused by
a bad video input on our new video recorder (same brand as the microscope,
oddly enough). When we used a different recorder, things worked
beautifully. We're smilin' again.

#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Fri, 15 Mar 1996 13:54:35 +1200
Subject: Creutzfeld-Jakob disease biohazards

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Dear Listserver readers,
We are a university EM unit primarily concerned with teaching and
supporting research across many disciplines. The local hospital have a
technician based in our Unit who processes their diagnostic cases for EM.
Recently it came to my attention that he had processed a brain biopsy from
a suspected Creutzfeldt-Jakob disease patient. What really concerned me was
that :
1) We had not been informed that the sample was in the lab
2) The hospital technician did not appear to be aware of the high-risk
nature of the disease.

This incident has highlighted two areas of concern in our Unit. Firstly
we have no mechanism in place that requires the hospital to inform us when
high risk biohazards come through our lab. Secondly, our mechanisms for
dealing with "high risk" samples and disposing of such material need to be
re-evaluated and improved.

My questions are:

How do other labs that are have high risk samples going through them
monitor the risk of the samples coming into the lab?

How do other labs handle and dispose of high risk biohazards?

Allan Mitchell
Richard Easingwood



Richard Easingwood
South Campus Electron Microscope Unit
School of Medical Sciences
University of Otago
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD















From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 15 Mar 1996 08:57:19 +0100
Subject: Re: TEM on archeol. etc.

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Message-Id: {n1385241999.36229-at-ematserv.ruca.ua.ac.be}
"microscopy-at-Sparc5.Microscopy.Co" {microscopy-at-Sparc5.Microscopy.Com}
X-Mailer: Mail*Link SMTP-QM 3.0.1

Reply to: RE} TEM on archeol. etc.

Dear Amy and others,
Good question. I have been looking (via the Current Contents for Physics and
Chemistry) for TEM studies on historical art and archeological material for
more than five years now and have never found any decent reference. It's
always SEM. Of course the fact that for TEM you'd have to destroy part of the
object is a serious problem. Nevertheless, as small quantities can be
sufficient I agree that it should be considered.
Could you please inform the group of the reactions that you get (maybe by
posting a compilation after a few weeks).
Thanks a lot.
Nick Schryvers








From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 15 Mar 96 08:36:53 EST
Subject: high-risk samples

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Message-id: {26557033-at-dancer.Dartmouth.EDU}

In this era of HIV and other potentially lethal contents of samples all of ours
are treated as high-risk.
Kate Connolly




From: Richard Lee :      richard_lee-at-QMGATE.ANL.GOV
Date: 15 Mar 1996 08:50:46 -0600
Subject: SMSI March meeting

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Message-ID: {n1385242375.2626-at-qmgate.anl.gov}
"Ankur Purohit" {ankur-at-anl.gov}
X-Mailer: Mail*Link SMTP-QM 3.0.2

Thanks Jonathan for agreeing to be the invited speaker on March 29. Copies of
the notice are on their way to you. The title,"Extremophiles - organisms at
the extremes" is intriguing. By the way can you explain for us what the CMB is
and does?
If you need info on how to get to McCrone Research Institute, I will send it
to you.

8:38 AM




From: Dave King (607)857-1248 T37/257-3 Ext DEKING-at-VNET.IBM.COM
Date: 15 Mar 1996 12:47:20 EST
Subject: 10 Micron Ti Section

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To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}
cc: BLAZEY --ENDVM1 Blazey, W.C. EMMI --ENDVM5 F. Emmi
WAITKUS --ENDVM1 Waitkus, C. CONROW --ENDVM5 K.M. (Karen) Conro


We're proud of our capabilities, but near 10 micron thickness
controlled over several mm is beyond our metallographic
sectioning capability and our microtome, both! We could work with
you on an experimental basis, but your goal is way out there.

{
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: geosclmr-at-showme.missouri.edu (Lou Ross)
Date: Fri, 15 Mar 1996 12:01:45 -0600
Subject: X-sectioning of filter paper.

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Thanks to all who responded.

Lou Ross

101 Geological Sciences Bldg.
University of Missouri
Columbia, MO 65211
(314) 882-4777, 882=5458 fax

'First came fire/Then came light/
Then came feeling/Then came sight'






From: STEELE-at-KRDC.INT.ALCAN.CA
Date: Fri, 15 Mar 1996 08:57:23 -0500 (EST)
Subject: Prep of ceramic fibres (TEM)

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--Boundary (ID g1sXwhSkJTbyydWcZFNosA)
Content-type: TEXT/PLAIN
Content-transfer-encoding: QUOTED-PRINTABLE

I'm guesing by Nestor's test message that this didn't get through the=
first=20
time, so I'll try again. Apologies to any who are receiving this for =
a second=20
time.

I received a few requests for details on my electroless Ni route for=
=20
fibre/powder preparation for the TEM. So here, culled from the lit., =
is the=20
method I've settled on:

---------------------------------------------------------------------=
--------=20

I'll start with the technique I use for electroless coating, then giv=
e a few=20
references for electroplating, and then the method I use. For ceramic=
powders I=20
I recommend electroless followed by electroplating.

Electroless Ni coating:

Apparatus: Hot plate / stirrer
3 stirring bars
Funnel, stand, Whatman 40 filter papers
4 250 ml beakers (glass) (Xtremely clean!!!)

3 solutions, A,B,C.

Solution A: Add 0.1 gm SnCl2 to 100ml water pH adjusted to 2 with=
HCl.
(sensitising) Use at rm temp.

Solution B: Dilute 2mls of a 5% PdCl2 solution to 100 ml.
(activator) Use at rm temp.

Solution C: Commercial Eleectroless solution made by HARSHAW. Ava=
ilable=20
through Engelhard technologies (416) 821 - 3325.
Comes in 3 parts Alpha 103 A-MB, Alpha 103 A-2, and=
=20
Alpha 103 A-1 replenisher. =20

Make up 100 mls by adding 20 ml A-MB, and 7.5 ml A-2,=
and=20
bringing up to 100 ml with D/I H2O. The replenisher =
you will
need if you use up all of the Ni, follow the destruct=
ions=20
given by Harshaw for gauging this, and replenishing.

This solution is used at 90 - 95=F8C.

Proc: Add aprox 1cc powder to solution A with stirring. Af=
ter=20
2 min. filter and rinse the cake with D/I H2O. Place=
the=20
filter cake and paper in solution B with stirring. A=
fter
2 min filter the sol and rinse the cake with D/I H2O.

Prepare a slurry by scraping the cake from the paper =
and=20
adding no more than 10ml H2O. Pour this into solutio=
n C,
with stirring, allow to plate for aprox. 1 min, then =
filter=20
and wash the powder.

And there you go.

Next, you incorporate the powder into a Eplated Ni sheet in your favo=
rite
plating bath (I use 80 ml Ni Sulphamate + 120 ml D/I H2O + 10 gm Bori=
c Acid +
1gm Nickel Chloride, + 5 drops Hydrogen Peroxide).=20

I use a stainless steel beaker, an "L" shaped cathode to drop the pod=
er on,=20
a high purity Ni anode, heat the sol to 50=F8C, and plate at 200 -300=
mA (2.5V)
DC. You need to plate a thin film(20 min), add just enough powder to=
=20
get an even dispersion, and then let it plate away for 3 to 4 hrs. T=
his=20
should result in a film somewhat in excess of 200=E6m.

A few key references:

S.D. Kirchoff and J. Y. Adkins, Metallography, 20 (1987) 75-87.

M.M. Morra, J. M. Morra, and R.R. Biederman, Materials SCiaence and E=
ngineering,
A124 (1990) 55-64

R. Baumann and M. J. Couper, Prakt. Met. 23 (1986) 140 - 148

R. D. Field and H. L. Fraser, Met. Trans. 9A, (1978) 131 - 134.

---------------------------------------------------------------------=
--------

###############################################################
# #
# Don Steele STEELE-at-KRDC.INT.ALCAN.CA #
# ALCAN INTERNATIONAL #
# Kingston Research and Development Center #
# P. O. Box 8400 #
# Kingston, Ontario Canada K7L 5L9 #
# #
###############################################################



--Boundary (ID g1sXwhSkJTbyydWcZFNosA)--




From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 15 Mar 1996 11:25:09 -0500 (EST)
Subject: Re: Creutzfeld-Jakob disease biohazards

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} Recently it came to my attention that he had processed a brain biopsy from
} a suspected Creutzfeldt-Jakob disease patient. What really concerned me was
} that :
} 1) We had not been informed that the sample was in the lab
} 2) The hospital technician did not appear to be aware of the high-risk
} nature of the disease.
}
} My questions are:
}
} How do other labs that are have high risk samples going through them
} monitor the risk of the samples coming into the lab?
}
} How do other labs handle and dispose of high risk biohazards?
}
Dear Allan & Richard,
Working at a state health department gives us a leg up on such
things. There are procedures in place for proper notification, handling
& disposal. We know what samples are being brought in for examination,
and since it is usually a physician who brings in any potentially danger-
ous specimens, we are made aware of any potential hazards. Usually, the
specimen has already been fixed, stained and embedded, which eliminates
the hazard. There are containers all around our lab for the disposal of
biohazards, and the safety office is responsible for their proper dispo-
sal. Having the hospital technician give you a list of specimens and
their condition (tissue, blocks, sections, etc.) before bringing them in
would be an obvious step. I can think of rationalizations for not doing
this, but maybe you can insist. If you have a safety office, by all
means get them involved. Good luck.
Yours,
Bill Tivol




From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Fri, 15 Mar 1996 13:55:59 -0800 (PST)
Subject: Re: Video Printer

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For decent video encoders (translators) at resonable cost ($200-$400 US) try
Harmonic Research in Paramus, New Jersey at (201) 652-3277.




From: ScottE57-at-aol.com
Date: Fri, 15 Mar 1996 21:04:18 -0500
Subject: Re: Video Printer

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199603152153.NAA26418-at-holonet.net}

The new Sony printers UP5600 will take PAL or NTSC signals - we handle a full
line - also you could use a frame grabber from a variety of manufactures that
take PAL in and let you output NTSC and use the existing printer. We carry
all these products, please call if you require further assistance.

Scott E. Berman
Advanced Imaging Concepts
Princeton, NJ
Phone(908) 274-1877 x26
Fax(908) 274-1974




From: EvexAnalyt-at-aol.com
Date: Sat, 16 Mar 1996 08:17:22 -0500
Subject: ISO Summary for Allied Signal Jordi Marti

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Message-Id: {n1385200787.59973-at-QuickMail.Yale.edu}

For those of you waiting for the ISO summary, Jordi Marti (at Allied Signal)
has been having problems with his E-Mail (he can't send mail out) for the
past two weeks. The summary will be mailed as soon as the E-Mail problems
are solved.

The above message was posted by Evex Analytical
as a courtesy for Jordi Marti, Allied Signal




From: James Patrick :      JPATRICK-at-OPUS.MCO.EDU
Date: Sat, 16 Mar 1996 16:30:47 -0500 (EST)
Subject: ISO Summary for Allied Signal Jordi Marti

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unsubscribe




From: alan-at-macro.mse.uiuc.edu (Alan E. Lucero)
Date: Sun, 17 Mar 1996 08:38:48 -0600
Subject: unsubscribe

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unsubscribe




From: Rune Sundset :      runes-at-fagmed.uit.no
Date: Sun, 17 Mar 1996 19:10:39 -0200
Subject: intracellular calcium

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Message-Id: {199603171807.TAA07477-at-petrus.fagmed.uit.no}
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I would like to know if there is a protocol out there for staining and
measuring intracellular calcium by using EM.

Best wishes,
Rune

======================================================================
Rune Sundset

Dept. of Medical Physiology
Inst. of Medical Biology
University of Tromsoe
N-9037 Tromsoe
Phone : +47 77 67 45 48 or +47 77 64 46 96 Fax : +47 77 64 54 40

----------------------------------------------------------------------
Private
Orneveien 12/213
N-9015 Tromso
Norway
Phone: +47 77 67 45 48
======================================================================





From: HENRY P ADAMS :      hadams-at-nmsu.edu
Date: Sun, 17 Mar 1996 12:05:37 -0700 (MST)
Subject: Fixation

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Fellow microscopists, I have to fix some algae (Cyanidium sp.) that are
growing at pH 1.8 for TEM with and without Ruthenium red, to stain
extracellular material. From what I have read glutaraldehyde fixes poorly
at low pH's and conventional fixative buffers do not work below pH 4 (P04).
Also,they can not tell me what the growth media is.
I thought I could add straight glutaraldehyde and then bring up the pH slowly
with NaOH or just rinse and fix normally under the assumption that the
ultrastructure will not be altered, initially, by a higher pH. Does anyone
have any experience with similar organisms or know
of any references that may guide me? I guess I am inclined to try several
approaches, but as is often the case the person has very limited funds
and I was hoping to use someone elses experience (experiments) to reduce
time and labor.
Thanks in advanced,
Hank Adams
EML, NMSU
Las Cruces, NM
hadams-at-nmsu.edu




From: slocombe-at-wsunix.wsu.edu (Peter Slocombe)
Date: Sun, 17 Mar 1996 19:13:40 -0800
Subject: Help! Leica or Zeiss?

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Perhaps you can help me with a personal dilemma; I'm about to purchase a
microscope and I've boiled
the choice down to either a Leica Galen III or a Carl Zeiss Standard 20.
The Leica has more bells and
whistles than the Zeiss, and for the setup I'm thinking of it's also about
$1000 cheaper than the Zeiss. I
have not used either and am 500 miles from the closest demo unit. In your
personal opinion would I be
better off, in the long-run, going with the Zeiss? Is one more comfortable
to use than the other in terms
of fatigue? How would YOU compare these two microscopes generally?

Peter Slocombe
Washington State University
slocombe-at-wsunix.wsu.edu





From: Stephan Helfer :      S.Helfer-at-rbge.org.uk
Date: Mon, 18 Mar 1996 09:47:38 BST
Subject: Re: Help! Leica or Zeiss?

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Dear Peter
Here at RBGE we have both Leica and Zeiss models, although we don't
have the Galen III. Traditionally we always had Leica models but when
they changed to infinity corrected optics, traditions didn't help any
more, and so we started looking afresh with an unbiased (obviously
infinity corrected) view. Our choice was Zeiss, partly because of
better optics although some of us find the Zeiss microscopes slightly
less user friendly, and I believe the Leica optics have improved now.
Here is my suggestion: You may live 500 miles away from a demo unit,
but why don't you ask both Leica and Zeiss to leave the microscope
with you for a week or so; this is the only way you can really judge
for yourself which one you would prefer for your own specimens. The
physics of microscopy are pretty well sussed out these days, so what
you want to pay extra for is durability (less trimmings normally
means less trouble) and ease of use (more trimmings sometimes help).

Cheers

Stephan

Yours sincerely

Dr Stephan Helfer, SSO
Mycologist / Plant Pathologist

Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
Scotland UK

http://www.rbge.org.uk

phone: +44 (0)131 552 7171 ext 280
or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
fax: +44 (0)131 552 0382
============================
1896
* BRITISH
* MYCOLOGICAL
* SOCIETY
1996
A century of fungal science
============================




From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 18 Mar 1996 10:04:59 EST
Subject: Re: Creutzfeld-Jakob disease

Contents Retrieved from Microscopy Listserver Archives
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} Has anyone heard of the slow viruses that can survive gluteraldehyde fixation?

The infectious agents that are able to survive fixation are the
prions. These agents contain protein only...no nucleic acid. Among
these are Creutzfeld-Jakob, scrapie, kuru,
Gersten-Straussler-Schenker, BSE (mad cow disease) and apparently
some forms of Alzheimers.

It is known that formaldehyde does not inactivate them, not surprising
since formaldehyde is one of the least cross-linking of all
fixatives, so far as proteins are concerned. My understanding is
that glut. does inactivate them, but don't take my word for it.

} On a similar note, there was an MSA-sponsored speaker a couple of years ago who
} was encouraging EM labs to make money by offering virus identification sevices
} to medical centers. The preparation protocol included advice to accept unfixed
} (ie. infectious), unidentified material and prepare negative stained samples on
} the bench in the laboratory.

We are a CLIA and State certified human clinical laboratory, licensed for
negative-stain virus identification. This has always been part of
our function as a veterinary EM lab, and for about 5 years we have
offered it as a service to the community. We do make a considerable
amount of money from it. We accept samples only from certain
reference laboratories and practitioners and these are only stool
samples, mostly (} 95%) from infantile diarrheas. Our submission form has an
entry for any unusual precautions that must be taken (ie HIV,
hepatitis, etc). Our understanding with our clients is that we don't
process such samples.
W. L. Steffens, Ph.D



Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: gwisler-at-asrr.arsusda.gov
Date: Mon, 18 Mar 1996 12:38:43 -0500 (EST)
Subject: Re: Creutzfeld-Jakob disease

Contents Retrieved from Microscopy Listserver Archives
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Title: Biological Science Technician (Plants)
Lab: USDA-ARS, Salinas, CA

The USDA-ARS is seeking a biological science technician (plants)
(GS-404-7, 8, or 9) for the Crop Improvement and Protection Research Unit
in Salinas, CA. The incumbent will share responsibilities in electron
microscopy of plant virus infections for ultrastructural characteristics.
The incumbent will also assist in research involving molecular, serological,
and biological studies of several plant viruses infecting sugarbeet and
vegetables. Candidate must have a knowledge of electron microscopy,
plant virology, and knowledge of microbiological techniques. Must be a U.S.
citizen. Bachelors degree is desirable. Salary is commensurate with
experience ($24,610-39,140 per annum).
For information regarding research program contact Gail C. Wisler or
James E. Duffus (408)755-2835. For information regarding application
procedures/forms contact Tom Nelson (408)755-2810. Applications must be
postmarked by May 6, 1996. The USDA is an equal opportunity employer.





From: azriel gorski :      azrielg-at-cc.huji.ac.il
Date: Mon, 18 Mar 1996 20:30:11 +0300 (GMT+0300)
Subject: Re: Help! Leica or Zeiss?

Contents Retrieved from Microscopy Listserver Archives
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I find it hard to believe that no such scopes have been *sold* closer
than 500 miles away. Ask your dealers, if they have sold such scopes, and
if they will contact those people and see if they will be willing to have
you inspect the scope.

Both companies make *excellent* products. At this quality, what feels
right for *you* is very important. So is the *service* you get from your
agent. Also check what scopes your facility has. The ability to "borrow"
a specific "widget" down the hall should be weighed in the equation.

As another user suggested, the agent may be able to lend you a scope to try.

All these conversations should also give you a pretty good idea of how well
you can work with the agent after the sale.

Good luck

Azriel Gorski, Head
Optical Microscopy Laboratory
Division of Identification and Forensic Science
Israel Police




From: Amy G Aslamkhan :      aslamkha-at-hawaii.edu
Date: Mon, 18 Mar 1996 10:22:34 -1000
Subject: TEM-Microwave fixation of marine organisms

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Does anyone have a protocol for microwave fixation of marine organisms?
I am considering this technique for fixing some crustacean tissue
(hepatopancreas), and a colleague of mine is considering microwave fixing
marine algae.

Amy Aslamkhan
University of Hawaii
aslamkha-at-hawaii.edu




From: biln-at-ozemail.com.au (bill neill)
Date: Mon, 18 Mar 1996 20:13:01 +1000 (EST)
Subject: em listserver

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I'd like to subscribe to the em listserver, please

Bill Neill
Leica Instruments





From: J-CRAIG-at-csu.edu (Joyce Craig)
Date: Mon, 18 Mar 1996 16:05:17 -0600 (CST)
Subject: bio-physics and microscopy

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We are attempting to start a bio-physics program at Chicago State University. We need feed back as to the role of physics in biological sciences problem solving. What is the trend? Is it accurate to say that biology and physics are becoming intertwined
? I personally see a need for a greater understanding of physics when working with the newer technology in electron microscopy in biological science but what do you think?





From: MSC18 :      MSC18.MSC18-LARS-at-bbsrc.ac.uk
Date: Mon, 18 Mar 1996 21:48:05 +0000
Subject: TEM: antibodies to anthocyanidins

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From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 18 Mar 1996 16:40:07 EST
Subject: Re: Creutzfeld-Jakob disease

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} As far as I know Alzheimers disease is not infectous---- Where did you get your
} information about Alzheimers disease?

This information is at least 4 years old and may not be correct.
About 4 years ago (maybe a little longer) there was a lengthy review
article in either New England Journal of Medicine or JAMA regarding
the slow human neurological disorders. At that time, there were at
least 5 recognized diseases that were classified as "Alzheimer" or
"Alzheimer-like". One of these was suspected to be of prion origin,
although by now it may be classified as something else.

One thing to consider if you are involved in studies involving human
(or any mammalian) brain tissue is that in years past, prion diseases
were commonly misdiagnosed as Alzheimers, and as humans (even
pathologists) are not perfect, this still may be the case.



W. L. Steffens, Ph.D
Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: jean ross :      jeanross-at-emiris.iaf.uiowa.edu
Date: Mon, 18 Mar 1996 12:55:06 -0600 (CST)
Subject: sectioning teeth

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I have been working on a project with a dental grad student involving
ultramicrotomy of undecalcified human teeth. The samples were prepared by
slicing them into approx. 100um slices and then embedding them in Epon. They
section just great but something happens when they're picked up on grids which
causes just the dentin portion of the section to wrinkle. I use formvar coated
200 mesh copper grids, stabilized with carbon and glow discharged. I spread the
sections out in the boat with chloroform (I have used xylene too) and have
picked them up every differnt way I could think of. Is it my support film?
Would a thicker section be desirable (sections are usually 90-95 nm)? It is
only the dentin part of the section that has this problem, the Epon and
composite resin reconstruction are perfectly flat. Does anybody have any
suggestions?

Thanks in advance,

Jean Ross
Central Microscopy Research Facility
University of Iowa
85 EMRB
Iowa City, IA 52242
(319) 335-8142
Web site: http://www.uiowa.edu/~cemrf







From: SMithP-at-agresearch.cri.nz
Date: Tue, 19 Mar 1996 17:36 +1200 (NZST)
Subject: Fluorescent in situs

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We are interested in trying some fluorescent in situ hybridisation on tissue
sections. We would be using fetal sheep tissue and would be probing with
riboprobes to growth factors and hormone receptors mainly.These probes
generally work well (as far as any in situ does) when radioactively
labelled, but we would be keen to get away from radioactive labels and would
really like to try multiple labelling. It seems very difficult to find
references describing the technique applied to tissue sections so any help,
advice ,sources of information or contacts would be much appreciated.

Thanks for your time

Peter Smith
AgResearch Wallaceville
PO Box 40063
Upper Hutt
New Zealand.

E-mail smithp-at-agresearch.cri.nz




From: Rune Sundset :      runes-at-fagmed.uit.no
Date: Tue, 19 Mar 1996 09:47:34 -0200
Subject: subscribe

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {199603190844.JAA22043-at-petrus.fagmed.uit.no}
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subscribe

======================================================================
Rune Sundset

Dept. of Medical Physiology
Inst. of Medical Biology
University of Tromsoe
N-9037 Tromsoe
Phone : +47 77 67 45 48 or +47 77 64 46 96 Fax : +47 77 64 54 40

----------------------------------------------------------------------
Private
Orneveien 12/213
N-9015 Tromso
Norway
Phone: +47 77 67 45 48
======================================================================





From: SveEn-at-pai.liu.se (Sverker Enestrom)
Date: Tue, 19 Mar 1996 09:13:29 +0100
Subject: Re: Creutzfeld-Jakob disease

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



} This information is at least 4 years old and may not be correct.
} About 4 years ago (maybe a little longer) there was a lengthy review
} article in either New England Journal of Medicine or JAMA regarding
} the slow human neurological disorders. At that time, there were at
} least 5 recognized diseases that were classified as "Alzheimer" or
} "Alzheimer-like". One of these was suspected to be of prion origin,
} although by now it may be classified as something else.
}
} One thing to consider if you are involved in studies involving human
} (or any mammalian) brain tissue is that in years past, prion diseases
} were commonly misdiagnosed as Alzheimers, and as humans (even
} pathologists) are not perfect, this still may be the case.

AD and prion diseases share the presence of amyloid precursor proteins
(beta-PP, APrP) but they are distinct diseases, both starting at the synapse
(beta-PP and PrP are proteins of the neuromuscular junction and CNS synapse).
In some of the prion diseases prion amyloid plaques are seen together with
paired helical filaments (NFT) making them "Alzheimer-like" as Budy writes.


*********************************************************
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
Fax: +46 13 13 22 57
*********************************************************






From: Reinhard Windoffer :      reinhard.windoffer-at-zoologie.uni-hamburg.de
Date: Tue, 19 Mar 1996 13:59:52 -0800
Subject: help: subscription to microscopy listserver

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Message-Id: {314F2E58.147A-at-zoologie.uni-hamburg.de}

Can you please send me the details for subscription to the microscopy
news group.


Reinhard Windoffer




From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 19 Mar 96 08:13:57 EST
Subject: Re: TEM-Microwave fixation of marine organisms

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Amy-
I don't know of any specific references to microwave fixation of crustacean
tissue, but you might want to experiment with the method developed by Bill &
Ruth Dewel (Appalachian State University) for fixation of tardigrades
(Echiniscus viridissimus). Please contact me by e-mail for the methodology.
General information on microwave fixation for TEM can be found at our WWW site
(http://www.ebsciences.com/).
Steven Slap, Vice-President
75767,640-at-compuserve.com





From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Tue, 19 Mar 1996 08:23:15 -0600
Subject: Re: Creutzfeld-Jakob disease

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Message-Id: {v01520d02ad74722277f6-at-[128.206.15.200]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Greetings,
My understanding was that you got prion diseases through eating
contaminated samples, hopefully uncommon in the world's em prep rooms. Is
there an airborne transmission route?
Thanks,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: MSC18 :      MSC18.MSC18-LARS-at-bbsrc.ac.uk
Date: Tue, 19 Mar 1996 14:47:20 +0000
Subject: TEM sorghum phytoalexins

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Hello,
I am currently doing TEM on sorghum leaves looking at the infection
process of Colletotrichum graminicola. Sorghum leaves produce
anthocyanidin phytoalexins in response to infection and I would like to
locate these compounds under the TEM. I was wondering if anybody out
there had or knew where I could get hold of antibodies to anthocyanidins,
specifically apigeninidin and luteolinidin, or if anyone knew of any stains
for EM which were specific to anthocyanidins.

Phill Wharton

*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*
Phillip Wharton
IACR-Long Ashton Research Station
Long Ashton
Bristol
BS18 9AH
*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*




From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 19 Mar 1996 12:53:43 -0500 (EST)
Subject: Re: bio-physics and microscopy

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} We are attempting to start a bio-physics program at Chicago State
} University. We need feed back as to the role of physics in biological
} sciences problem solving. What is the trend? Is it accurate to say
} that biology and physics are becoming intertwined? I personally see a
} need for a greater understanding of physics when working with the newer
} technology in electron microscopy in biological science but what do you
} think?

Dear Joyce,
Physics has several roles in bio problem solving. There is the
application of physical measurements (ultracentrifugation, calorimetry,
diffraction to name just a few), the use of physics in understanding what
happens to the specimen (radiation damage in EM, physical preparation
techniques like microwaving & sectioning), and, perhaps most important,
the basic viewpoint of physics can aid thinking about bio phenomena--
this has proved to be especially valuable to me. I found that my training
in physics gave me the quantum-mechanical viewpoint from which to view
enzyme-substrate interactions and molecular structure--which gave me in-
sights that my colleagues not trained in physics did not have.
I believe the trend in research is toward interdisciplinary studies,
so physics, among other disciplines, is being applied more and more to other
fields, including biology. I would not single out the bio-phys linkage as
unique (or even one-way). I think that all disciplines are becomming more
fluid with the boundaries getting fuzzier rather than that biology and
physics specifically becomming intertwined.
Understanding the principles of the instrumentation is always a
plus, and the newer technology certainly relies on physical principles.
On the other hand, the newer instrumentation is also becomming more user-
friendly and computer-oriented. This means that the instruments can be
operated merely by clicking on a few menus, and repair of a faulty in-
strument usually means replacing a computer chip rather than disassembling,
analysing which part is broken, making a new part and reassembling. At the
latter level, less understanding of physics may be required to perform the
experiment, but more required to understand it.
Yours,
Bill Tivol





From: Gary Login :      glogin-at-bih.harvard.edu
Date: Tue, 19 Mar 1996 13:45:40 -0500
Subject: TEM-Microwave fixation of marine organisms

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In message {Pine.SV4.3.91.960318100758.25965A-100000-at-uhunix5} Amy G Aslamkhan
writes:
} Does anyone have a protocol for microwave fixation of marine organisms?
} I am considering this technique for fixing some crustacean tissue
} (hepatopancreas), and a colleague of mine is considering microwave fixing
} marine algae.
}
} Amy Aslamkhan
} University of Hawaii
} aslamkha-at-hawaii.edu


Microwave fixation is an ideal method to preserve the soft tissue within a hard
(calcified shell). References appropriate to your application follow:

Berg, C. J., and N. L. Adams. Microwave fixation of marine invertebrates. J Exp
Mar Biol Ecol 74: 195-199, 1984.

Login GR, Dvorak AM. Methods of microwave fixation for microscopy. A review of
research and clinical applications: 1970-1992. Prog Histochem Cytochem
1994;27/4: 1-127.


If you need further assistance or methodological details please do not hesitate
to contact me by calling or sending a fax to the number at the end of this
E-mail message.

Gary Login



Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676





From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 19 Mar 1996 14:05:57 EST
Subject: Re: Creutzfeld-Jakob disease

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} My understanding was that you got prion diseases through eating
} contaminated samples, hopefully uncommon in the world's em prep rooms. Is
} there an airborne transmission route?

The review article that I described earlier notes some cases of prion
disease in neurosurgeons who had performed procedures on known
infected patients in years past.
Not exactly iron-clad evidence of alternative transmissibility....but
still.

This thread had gotten my interest on the subject piqued again. I'll
see if I can dig out the original article.




W. L. Steffens, Ph.D
Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: lucina-at-xk.com (Lucina Mastro)
Date: Tue, 19 Mar 1996 12:37:33 -0800
Subject: subscribe

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subscribe






From: kna101-at-utdallas.edu
Date: Tue, 19 Mar 1996 14:04:58 -0600 (CST)
Subject: Re: sectioning teeth

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Jean:

I've come across a similar problem with epon embedded inner ear
(cochlea). In my case, the soft tissue I need to look at is completely
surrounded by decalcified bone. Several things I've tried seem to help
with this problem: trim away the bone from the surface of the block
before sectioning or increase infitration times, using a harder epon
mix to try and reduce the difference in hardness that occurs in the
block between pure epon, bone and soft tissue. I hope this helps.
P.S. I've had this happen even on bare grids.

Karen Pawlowski
Technician, UT Southwestern Medical Center
PhD student, UT Dallas




From: yimei-at-befvax.uchicago.edu
Date: Tue, 19 Mar 1996 16:47:42 EST
Subject: Subscribe

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Subscribe Yimei-at-befvax.uchicago.edu




From: Paul Webster :      paul.webster-at-yale.edu
Date: 19 Mar 1996 18:20:46 -0500
Subject: Re: Creutzfeld-Jakob disease

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Message-Id: {n1384862484.65115-at-QuickMail.Yale.edu}

} My understanding was that you got prion diseases through eating
} contaminated samples, hopefully uncommon in the world's em prep rooms. Is
} there an airborne transmission route?

The most common form of transmission for toxoplasma gondii is from eating cat
feces but I am sure no-one would admit to eating that!



Paul Webster, Ph.D.
Center for Cell Imaging
Yale University School of Medicine





From: Jill Craig :      jcraig-at-unbc.edu
Date: Tue, 19 Mar 1996 15:21:46 -0800 (PST)
Subject: unions

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Hi,

The University here has recently decided to unionize the staff. I was
wondering if SEM, NMR, MS technicians are commonly unionized in the
experiences of the group. We were hired into our designated positions
and there really isn't a ladder to climb to higher positions or
designations. Overall, I'm having a difficult time seeing the advantage
of being part of a union although I'm fully aware of the price deducted
from my salary. Is this a common practice in Canada or elsewhere?

Reply to me and I'll summarize.

Thanks,

Jill




From: Colin MacRae :      C.Macrae-at-minerals.csiro.au
Date: Wed, 20 Mar 1996 10:52:25 +1000
Subject: Angle measurement of single crystal faces

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Message-Id: {199603191826.NAA12061-at-hoh.mbl.edu}

I am using an In_lens Field Emission SEM to image various single crystals.
Can anyone suggest a technique for measuring the angles between single
crystal faces? (Since the IFESEM is an in-lens microscope use of a double
detector system is impossible)

Is it possible to take secondary electron stereo pair images and measure the
true crystal face angles?

Colin MacRae
Scanning Probe Microscopy and Electron Microscopy Section

Commonwealth Scientific and Industrial
Research Organisation. _--_|\ cmac-at-minerals.csiro.au
Division of Minerals / \ +61 3 9647 0296
PO Box 124, Port Melbourne 3207 \_.--._/ fax 61 3 9646 3223
AUSTRALIA

EM units WWW site http://www.minerals.csiro.au/em-unit/





From: Scott D. Whittaker :      sdw-at-biotech.ufl.edu
Date: Tue, 19 Mar 1996 22:49:02 GMT
Subject: Re: Creutzfeld-Jakob disease

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At 06:20 PM 3/19/96 -0500, you wrote:
} } My understanding was that you got prion diseases through eating
} } contaminated samples, hopefully uncommon in the world's em prep rooms. Is
} } there an airborne transmission route?
}
} The most common form of transmission for toxoplasma gondii is from eating cat
} feces but I am sure no-one would admit to eating that!
}
}
}
Personally I find a Snickers more satisfying. I have
been told oth by my Vet and our OB/GYN that it is possible to contract the
organism through the dust kicked up while kitty uses his litterbox not to
mention when we have to change it.
{} {} {} {} {} {} {} {} {} } {} {} {} {} {} {} {} {} {} {} {}
Scott Whittaker Ph 352-392-1295
ICBR EM Core Lab Fax 352-846-0251
University of Florida e-mail sdw-at-biotech.ufl.edu
www http://www.biotech.ufl.edu/~emcl/





From: scott_l-at-unin1.unorth.ac.za
Date: Wed, 20 Mar 1996 07:39:54 -0200
Subject: TEM and SEM training courses

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Message-ID: {n1384845499.69898-at-msmail.tmc.tulane.edu}

Hello from the southern tip of Africa

I am emploted at an EM facility at the University of the North, South Africa.
We have been running an EM course for postgraduate students for the past 3
years. It is part of a techniques course for fourth year students in the
biological sciences, and serves as an introductory course. Following their
training, many students include ultrastructural studies as part of their
projects

I am quite eager to year of institutions that are involved in EM training.
In particular, I am interested in course content, training facilities (in-
cluding instruments), and whether or not such a course improved past
students chances of employment or to get sponsorship for futher studies.

Leon Scott
EM Unit
Univ. of the North
Private Bag X1106
Sovenga
Republic of South Africa
tel 27 152 2682957
fax 27 152 2683156/2682893




From: CAA3045-at-ritvax.isc.rit.edu (Ciprian A. Almonte)
Date: Wed, 20 Mar 1996 00:21:20 -0500
Subject: Student looking for summer job

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Dear Fellow Microscopists,
I'm currently a biomedical photographic student at the Rochester
Institute of Technology. I have a BS in Biology and I'm currently
pursuing a second degree in Biomedical Photographic with a concentration in
photomicrography. My career objective is to apply my knowledge in biology
and biophotography to the documentation of medicine and the biological
sciences. You can view my resume and some of my images in my web site at
http://www.isc.rit.edu/~caa3045.
The biomedical photographic Communication department at RIT has
exposed me to many photography techniques. I am experience with many
formats of films processes, black-and-white, and color printing,
photomacrography, photomicrography, darkfield, brighfield, nomarski,
phase-contrast, polarizing, Rheinberg, SEM, and fluorescence microscopy.
Also, I have a great interest and hands on experience in digital
photography and computer (Macintosh major area of strength).
Also, I have a lot of experience with several immnunocytochesmistry
techniques, and the Patch-clamp techniques.
If you think I may contribute to your department send me an e-mail
to "caa3045-at-rit.edu" or "almonte-at-medcolpa.edu"
Thanks,

__________________________________________________________
Ciprian A. Almonte
Rochester Institue of Technology
Biomedical Photographic Communications
Rochester, NY 14623-5603
Visit my web site at http://www.isc.rit.edu/~caa3045/
__________________________________________________________






From: Gejing Li :      gjinli-at-umich.edu
Date: Wed, 20 Mar 1996 10:31:51 -0500 (EST)
Subject: subscribe

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Message-ID: {199603201324.IAA08945-at-IndyNet.indy.net}
To: Colin MacRae {C.Macrae-at-minerals.csiro.au} ,
Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

Please add my name on your mailing list. Thanks.




From: kaurin-at-rmslab.rockefeller.edu
Date: Wed, 20 Mar 1996 11:05:40 EST
Subject: Web employment listings?

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Could someone direct me to the website that has jobs/resume postings?
Thanks, Shelley Landon Kaurin





From: RobertCO2-at-aol.com
Date: Wed, 20 Mar 1996 12:18:21 -0500
Subject: subsribe

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I am interested in subscribing to the EM news group.
Robert Sherman
"robertco2-at-aol.com"




From: howard-at-cshl.org (Tamara Howard in Cold Spring Harbor Laboratory)
Date: Wed, 20 Mar 1996 11:17:38 -0500
Subject: TEM:Eppendorfs & LR White?

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Quick question: are Eppendorfs air-tight enough to use as embedding
capsules for LR White? I have cell pellets to embed and would rather not
disrupt them if I can avoid doing it.
Thanks!
Tamara






From: alan-at-macro.mse.uiuc.edu (Alan E. Lucero)
Date: Wed, 20 Mar 1996 13:55:47 -0600
Subject: unsubscribe

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unsubscribe




From: RNBALDUC-at-ARCRIDE.EDU.AR
Date: Wed, 20 Mar 1996 17:39 -0300
Subject: unsubscribe

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Microscopists

The Laboratory of Electron Microscopy, School of Bioengineering,U.N.E.R
Argentina, is look for SEM to received it in donation or a very low price.
Only is required the technical information of your " work state", the model and
year of construction.
We pay all the costs of shipment
Please, for any questions contact me in

RNBALDUC-at-arcride.edu.ar

thanks in advance.





From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 20 Mar 96 16:25:16 EST
Subject: TEM:Eppendorfs+LR White

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential

No plastic capsule or tube that I know of is air-tight enough for LR White.You
may double emded with gelatin and get away with a plastic capsule.
Kate Connolly




From: masumura-at-anvil.nrl.navy.mil (bob masumura)
Date: Wed, 20 Mar 1996 09:43:23 -0500
Subject: help

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From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Wed, 20 Mar 1996 14:02:27 -500
Subject: TMV Particles

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O.k. does anyone out there know where to get a vial of TMV
(Tobbacco Mossaic Virus) from for use as a mag standard and
particulate test specimen?

Carolina Biological is the only source I have come across but they
sell it as a whole kit for learning about Plant viruses, and I just
want the virus particles.





From: RMacKay :      RMACKAY-at-AC.DAL.CA
Date: Wed, 20 Mar 1996 11:22:08 +0000
Subject: EMPA garnet analysis

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Message-Id: {199603201527.LAA19788-at-Snoopy.UCIS.Dal.Ca}
Comments: Authenticated sender is {RMACKAY-at-ac.dal.ca}


A colleague and I were discussing microprobe analysis of garnets and
he remarked that their analysis of Almandine often produce high totals,
a phenomenon I have also observed with high Fe garnets. I know that
others have observed this as well but has anyone come up with an
explanation ?

Bob MacKay
Robert MacKay
Department of Earth Sciences
Dalhousie University
Halifax, Nova Scotia, Canada
B3H 3J5
Tel: 902 494-7087
e-mail rmackay-at-ac.dal.ca




From: W.L. Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Wed, 20 Mar 1996 16:40:56 EST
Subject: Creutzfeld Jakob

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For those of us following this thread, the following statement issued
today by USDA-APHIS will be of interest.

} The British Ministry of Health and Agriculture have announced today that
} they may have associated CJD to BSE - an announcement was made to the
} House of Commons today.

I suppose in the future, we may be refusing to allow bovine tissues into
our labs.

-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Chandra S. Pande :      Pande-at-anvil.nrl.navy.mil
Date: Wed, Mar 20, 1996 9:55 AM
Subject: POST DOCTORAL POSITONS AT NAVAL RESEARCH LABORATORY,WASH DC

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Message-Id: {199603201429.IAA09354-at-Sparc5.Microscopy.Com}

Post doctoral positions are available at the Naval Research
Laboratory in the area of materials characterization of
superconductors for U.S. citizens with a Ph.D. in metallurgy,
physics or chemistry. Techniques utilized are TEM, SEM, SAM and
optical microscopy. Most modern instruments in these fields such
as a new CM-30 TEM are available including access to large scale
computational facilities Previous experience in superconductivity
is not required but expertise in transmission electron microscopy
is necessary. For further details, please contact:
Dr. Chandra Pande
Code 6320 tel:(202).767-2744
Naval Research Laboratory fax:(202) 767-2623
Washington, DC 20375-5343 e-mail:pande-at-anvil.nrl.navy.mil



Chandra S.Pande email: pande-at-anvil.nrl.navy.mil
phone: (202) 767-2744
fax: (202) 767-2623
Mailing address:
Code 6325
Naval Research Lab
Washington DC 20375 USA





From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Wed, 20 Mar 1996 18:10:12 -0500 (EST)
Subject: Re: TEM:Eppendorfs & LR White?

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On Wed, 20 Mar 1996, Tamara Howard in Cold Spring Harbor Laboratory wrote:

} Quick question: are Eppendorfs air-tight enough to use as embedding
} capsules for LR White? I have cell pellets to embed and would rather not
} disrupt them if I can avoid doing it.
} Thanks!
} Tamara
}

We routinely embed material in eppendorfs. They work fine.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************

}
}




From: krogers-at-ecn.purdue.edu (Kirk Rogers)
Date: Wed, 20 Mar 1996 13:46:21 -0500
Subject: Image Analysis Short Course

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Forwarded from the NIH-Image list-

Thought many might be interested.

} Date: Wed, 20 Mar 1996 08:37:18 -0600 (CST)
} Precedence: bulk
} From: DrJohnRuss-at-aol.com
} To: Multiple recipients of list {nih-image-at-Soils.Umn.EDU}
} Subject: Image Analysis Short Course
} X-Comment: NIH Image Distribution List
}
} Apologies to those who have already read this. I posted it several months ago
} but from the number of phone and e-mail inquiries it seems worthwhile to put
} it up again.
}
} The intensive short course on image analysis and stereology that North
} Carolina State University has presented for many years will be offered three
} times in 1996, twice in Raleigh, NC (May 16-18 and May 20-22) and once in
} Denmark (June 12-15). The textbook for the course is "The Image Processing
} Handbook, 2nd Edition" by John C. Russ. Attendees also receive a CD-ROM
} (usable on both Macintosh and Windows) containing many images and a
} comprehensive set of plug-in modules for Adobe Photoshop that implement all
} of the various processing and measurement algorithms discussed in the book,
} with a detailed graphic step-by-step tutorial on disk. The faculty for the
} course include John Russ (NCSU), Robert Dehoff (Univ. of Florida) and
} Jeanette Norden (Vanderbilt Univ). This is a good opportunity to learn the
} fundamentals of quantitative image analysis as well as get answers to
} specific problems, understand how and when to use various methods and
} algorithms, and how to interpret results in a variety of fields including
} materials science, geology, biology and medicine, forensics, food science,
} etc. The course includes hands-on laboratories and the opportunity to work
} with attendees' images.
}
} For more details, you may request the printed brochure by calling Belinda
} Niedwick at NCSU Dept. of Continuing and Professional Education,
} 919-515-8185. There is also a web site with detailed course description and
} on-line registration at http://vims.ncsu.edu/matsci/IPCourse.html
}
} Thanks for your interest.
} John Russ
}

-Kirk
_____________________________________________
Kirk Rogers krogers-at-materials.ecn.purdue.edu
OR kirk.a.rogers.1-at-purdue.edu
Purdue University, School of Materials Science and Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289
OFFICE: 317-494-8751 FAX: 317-494-1204
http://materials.ecn.purdue.edu/~krogers






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Wed, 20 Mar 1996 17:06:18 GMT
Subject: Re: TEM:Eppendorfs & LR White?

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} Quick question: are Eppendorfs air-tight enough to use as embedding
} capsules for LR White? I have cell pellets to embed and would rather not
} disrupt them if I can avoid doing it.
} Thanks!
} Tamara
}
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

Yes, as long as you nearly fill the tube with resin to exclude as much air
as possible
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Thu, 21 Mar 1996 14:42:04 +1100
Subject: Re: TEM:Eppendorfs & LR White?

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} Quick question: are Eppendorfs air-tight enough to use as embedding
} capsules for LR White? I have cell pellets to embed and would rather not
} disrupt them if I can avoid doing it.
} Thanks!
} Tamara


They're fine, though as always happens there will possibly be a squishy bit
at the top of the tube where the LRW hasn't hardened.

Diana van Driel
Dept Ophthalmology
Sydney University
AUSTRALIA 2006






From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Wed, 20 Mar 1996 17:21:42 -0500
Subject: Re: Web employment listings?

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Message-Id: {199603202220.RAA28105-at-lihti.org}
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} Could someone direct me to the website that has jobs/resume postings?
} Thanks, Shelley Landon KaurinDear Shelley:

The new search engine, ALTA VISTA, is good for this type of thing. In
addition to a vast text index of web pages, it can also search
usenet-including the newsgroups where most jobs are posted. Address is
http://www.altavista.digital.com; if you have problems (some browsers
return a blank screen), we have a direct link to it from our "Links" page
(http://www.tiac.net/users/everlast/nano/Links.html, in the "Internet and
WWW directories" section. Try a simple query with something like "Jobs
offered microscopy" with the search field set to "Usenet".

On the Web, try Career Mosaic (http://www.careermosaic.com), which indexes
many of the jobs offered newsgroups. This has links to places to post
resumes as well.

Regards,

Rick Powell

*****************************************************************
* NANOPROBES, Incorporated *
* 25 East Loop Road, Suite 124, Stony Brook, NY 11790-3350, USA *
* *
* http://www.tiac.net/users/everlast/nano/home.html *
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From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 20 Mar 1996 17:03:43 -0500 (EST)
Subject: Re: Angle measurement of single crystal faces

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} I am using an In_lens Field Emission SEM to image various single crystals.
} Can anyone suggest a technique for measuring the angles between single
} crystal faces? (Since the IFESEM is an in-lens microscope use of a double
} detector system is impossible)
}
} Is it possible to take secondary electron stereo pair images and measure the
} true crystal face angles?
}
Dear Colin,
It is certainly possible to reconstruct the 3D volume from
a stereo pair, but that is not very accurate and you would need the appro-
priate software to perform the calculation. If you have a double-tilt or
tilt-rotation specimen holder and can manipulate the specimen until one of
the faces is normal to the beam, then re-orient so that the second face is
normal to the beam, then you can get the angle between the faces more accu-
rately if you can record angular positions for the two orientations.
Yours,
Bill Tivol




From: george.braybrook-at-ualberta.ca (George Braybrook)
Date: Wed, 20 Mar 1996 16:37:45 -0700
Subject: Re: Oil Sample

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Mark,

If you can sputter coat the sample with gold, you can probably get
them to pump down well enough to look at them in the SEM. We used to look
at tar sands samples with a Cambridge S250 turbo SEM after they were placed
in a vacuum dessicator for a few days to remove the light fraction of the
oil. However you will inevitably get some contamination of the column
when the beam heats up the oil, especially during x-ray analysis, so plan
to do a column clean shortly after running the oil samples.
The best way to run these types of samples is with a cryo stage.
Freeze the little buggers so they can't mess up your aperatures!!!



} I have an Amray 1600 Turbo SEM, I have a question about a sample
}
} that I would like to observe. The sample is an oil that a plant
}
} uses for a dosing machine. There are visible spots in the oil that we
}
} suspect may be Hg or Na, or the oil may be a synthetic oil, one that was
}
} not supposed to be used in the first place. I was asked if it is possible
}
} to examine a smear of the oil under SEM/EDX and identify the spots in it,
}
} but I'm thinking no because of the wet oil contaminating my sample chamber,
}
} and also the temperature of the electron beam may volatile the mercury.
}
} Is it not possible to observe this oil with my instrument? Are there any
}
} suggestions that I may follow?
}
}
} Mark Darus
} General Electric Co.
}
} Darus-at-cle.dnet.ge.com

Cheers
:)
George
Department of Earth and Atmospheric Sciences
University of Alberta
Edmonton, Alberta T6G 2E3
Canada
ph: 403-492-5746
fax: 403-492-2030






From: Pecz Bela :      pecz-at-falcon.mufi.hu
Date: Thu, 21 Mar 1996 08:46:23 +0100
Subject: subscribe

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Please put my name onto your mailing list (microscopy news).
Thanks in advance, Bela Pecz
21st March 1996


-----------------------------------------
Dr. Bela Pecz
Research Institute for Technical Physics
H-1325 Budapest, POBox 76
Hungary
phone: 36-1-1698-961
fax: 36-1-1698-037
E-Mail: pecz-at-falcon.mufi.hu
-----------------------------------------





From: Stephan Helfer :      S.Helfer-at-rbge.org.uk
Date: Thu, 21 Mar 1996 09:42:01 BST
Subject: Re: TEM:Eppendorfs & LR White?

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microscopy-at-Sparc5.Microscopy.Com

Dear Tamara
I have used LR White in three sizes of Eppendorfs (0.5, 1 & 2ml or
thereabouts). Air did not seem to be a problem, but the plastic used
in the tubes must have reacted with the resin. The smallest size tube
resulted in strangely shaped blocks, the bigger ones seemed ok. I
didn't persue the matter, but I could imagine that coating the inside
of the tubes with gelatine might help preventing any reaction (I also
used gelatine capsules inside Eppendorfs which worked very well).
Hope this helps

Yours sincerely

Dr Stephan Helfer, SSO
Mycologist / Plant Pathologist

Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
Scotland UK

http://www.rbge.org.uk

phone: +44 (0)131 552 7171 ext 280
or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
fax: +44 (0)131 552 0382
============================
1896
* BRITISH
* MYCOLOGICAL
* SOCIETY
1996
A century of fungal science
============================




From: wagner-at-natlab.research.philips.com
Date: Thu, 21 Mar 1996 13:19:16 +0100 (MET)
Subject: subscribe?

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Hi,

I'd like to subscribe to the microscopy dicussion list,

Thanks,

Raymond Wagner.

-----------------------------------------------------------------
Raymond Wagner wagner-at-natlab.research.philips.com
Philips Research Laboratories Building WY 4.53
Prof. Holstlaan 4 Mailbox WY 42
5656 AA Eindhoven Phone +31 40 27 44573
The Netherlands Fax +31 40 27 43478




From: dago-at-odyssee.net (Dagoberto Rodriguez)
Date: Thu, 21 Mar 1996 08:56:29 -0500
Subject: Re: Oil Sample

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I would filter the sample trough a membrane filter (Millipore?) and wash
with a suitable solvent (n=hexane?) to eliminate the oil. Then dry and
carbom coat.
The residue in the membrane should be easily observed in SEM.
Dagoberto Rodriguez
-------------------------

} I have an Amray 1600 Turbo SEM, I have a question about a sample
}
} that I would like to observe. The sample is an oil that a plant
}
} uses for a dosing machine. There are visible spots in the oil that we
}
} suspect may be Hg or Na, or the oil may be a synthetic oil, one that was
}
} not supposed to be used in the first place. I was asked if it is possible
}
} to examine a smear of the oil under SEM/EDX and identify the spots in it,
}
} but I'm thinking no because of the wet oil contaminating my sample chamber,
}
} and also the temperature of the electron beam may volatile the mercury.
}
} Is it not possible to observe this oil with my instrument? Are there any
}
} suggestions that I may follow?
}
}
} Mark Darus
} General Electric Co.
}
} Darus-at-cle.dnet.ge.com
}
}





From: howard-at-cshl.org (Tamara Howard in Cold Spring Harbor Laboratory)
Date: Thu, 21 Mar 1996 09:26:19 -0500
Subject: TEM:LRW/Eppendorfs revisited

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Thanks to everyone who responded to my call for help. In case anyone is
interested, the replies split about 70/30 in favor of Eppendorfs, but
several people said they'd never been able to get them to work for LRW.
There were a few who suggested using gelatin capsules with the Eppendorfs -
as additional air barriers. I'll see how the plain tubes work, then try the
elaborate set-ups as needed.
Thanks again!
Tamara






From: HENRY P ADAMS :      hadams-at-nmsu.edu
Date: Thu, 21 Mar 1996 08:26:14 -0700 (MST)
Subject: Re: TEM:Eppendorfs & LR White?

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On Wed, 20 Mar 1996, Tamara Howard in Cold Spring Harbor Laboratory wrote:

} Quick question: are Eppendorfs air-tight enough to use as embedding
} capsules for LR White? I have cell pellets to embed and would rather not
} disrupt them if I can avoid doing it.
} Thanks!
} Tamara
Tamara: it works ok, but removing them from the eppenddorfs can be tricky
sometimes. Also, if your pellets are too dense, poor infiltration and
polymerization can be a problem.
Why not embed them in agar first in the tubes then transfer them..} } }
Hank Adams, NM






From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Thu, 21 Mar 1996 12:28:30 -0500 (EST)
Subject: actin

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I am startint to look at tapeworms in the Trypanorhynch group and was
wondering if someone has a suggestion for a good fix for the preservation
of "actin". I normally use 2.5% glut in cacodylate buffer. Will this be
alright to use? I greatly appreciate any help.

Peace,

Phil Rutledge




From: js_vetrano-at-ccmail.pnl.gov
Date: Thu, 21 Mar 1996 07:55 -0800 (PST)
Subject: List server

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Dear madam/sir

I read that you run a list server for microscopy. I believe Larry Thomas from
our lab subscribed but now that he has retired I would like to begin receiving
this service. Do I need to do anything?

Regards,

John Vetrano
SUBSCRIBE
js_vetrano-at-pnl.gov




From: HENRY P ADAMS :      hadams-at-nmsu.edu
Date: Thu, 21 Mar 1996 12:09:24 -0700 (MST)
Subject: Variable pressure SEM: fresh plant tissue

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We recently acquired a variable pressure sem (Hitachi S3200N) with a Fullam
cold-stage, a researcher wants to image fresh leaves and is particularily
interested in the status (open vs closed) of the stomates. Does anyone
have any suggestions as to temp, pressure, Kv, beam current, etc?

TIA,
Hank Adam






From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Thu, 21 Mar 1996 14:47:27 -0600
Subject: Re: TEM:LRW/Eppendorfs revisited

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Message-Id: {v01520d07ad777008175c-at-[128.206.15.200]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Greetings,
Tamara wrote:

} Thanks to everyone who responded to my call for help. In case anyone is
} interested, the replies split about 70/30 in favor of Eppendorfs, but
} several people said they'd never been able to get them to work for LRW.
[snip]
Just wanted to point out that the term "Eppendorf tube" is a bit
like "whiskey", understood instantly, but in fact refering to a wide range
of product. So, "ep tubes" are made by gazillions of companies and
presumably out of, well, dozens, of different kinds of plastics. Might be
worth finding out from someone in the successful camp exactly where they
bought their ep tubes.

Tobias

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Thu, 21 Mar 1996 09:00:32 GMT
Subject: Neg. Processor Summary (long)

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Here is a summary of the processor discussion. Contact the person who posed
the question to get the responses that didn't get posted to the list. In the
future, we maintain a web site in which we archive most of the discussions
for future reference. Go to the web site listed at the bottom of this page
and look in "Tips & Tricks". We have added a new search feature and would
welcome any input.




At 01:29 PM 3/20/96 -0500, you wrote:
} Not long ago, photo processors were discussed by this group. Of course
} as soon as the last message on this subject went into my trash, our
} negative processor broke and is beyond repair. It is a vintage processor
} by Hope (Model 152 B&W13-10), and spare parts are not anymore available.
} Also it was not always obvious in the discussion, if the subject was
} negative or positive processors. Please, if anyone has a summary of the
} results, I would appreciate to receive a copy by e-mail. Of course, I
} welcome any new information as well.
}
} Thanks very much,
}
} Hasso Weiland
} Alcoa Technical Cneter
} Alcoa Center, PA 15069
}
---------------------------------------------
Considering that photography is still so important to our profession, it is
likely that in this group are a few critical individuals with personal
experiencee using dry-to-dry black and white photographic processors. Would
anyone care to recommend a counter-top processor that they are happy with? I
wouldn't object to knowing which ones to stay away from either.

Many thanks,

Doug Keene
Shriners Hospital for Crippled Children
Portland, Oregon

--------------------------------------------------
} We have
been using the Ilford 2150 RC for over a year, maybe two by now.
The results are quite good, however there have been mechanical problems with
it from the get go. We had to replace bearings early on and have had to
dismantle and re-assemble the rollers over and over to keep it from
squeaking and sqealing and grinding. So it has been a mixed bag for us. We
do about 10,000 prints a year and it has been a real time-saver too
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-

------------------------------------------------------

The best processor out there is the Mohrpro. We have been using one
for about 8 years and recommend it when ever we can. It is easy to
use and maintain, 2 min. dry to dry, permanent (not stabilized) and
uses RC paper and Kodak Fixer. We do purchase the developer from
Mohr. The current cost for paper up to 8" width is $4295, the
Mohrpro8. and 14" width $4800, the Mohrpro14. (They do sell
rebuilt machines as well). They stand behind their product and I can
only say great things about Mohr.
For information contact:
Bob or Jim Jackson (tell them Linda at Loyola sent you!)
Mohr Enterprises
65 E. Palatine Rd. Suite 103
Prospect Heights, Il 60070
1-847-465-0048

Linda Fox Loyola University Medical School
lfox1-at-wpo.it.luc.edu

----------------------------------------------------------------

Dear Doug:
We've had our Mohr Pro8 for about 9 months and love it. This model came
highly recommended by other users in the Boston area who had used them
much longer than us. We now wonder how we put up with the tray method
for so long! If you want more info, E-mail or call me.
Don Gantz
Boston Univ School of Medicine
gantz-at-med-biophd.bu.edu
617-638-4017

PS. AGFA also makes one but have no details.

----------------------------------------------------------------

I have used an Agfa system, chemistry and paper for 12 years. The processor
was not sufficiently robust nor reliable for us. In any case it is no longer
available. The Agfa Variable Contrast Premium paper is my favorite emulsion
for scientific work. I recommend that you consider the Durst Printo system.
It is modular so you can build it to suit your own needs. It is my choice of
currently available systems.

: "Larry D. Ackerman" {mishot-at-itsa.ucsf.edu}
-------------------------------------------------------------------

At 10:12 AM 3/7/96 -0600, you wrote:
} The best processor out there is the Mohrpro. We have been using one
} for about 8 years and recommend it when ever we can. It is easy to
} use and maintain, 2 min. dry to dry, permanent (not stabilized) and
} uses RC paper and Kodak Fixer.

I must respectfully disagree. I was personally responsible for implementing
both an Ilford 2150 and a Mohr Pro 8" Model in a multiuser lab. While I
liked the Mohr and our users were mostly happy with it, from an ease of use
and maint. point of view, I would have to go with the Ilford.

The 2150 ran flawlessly for several years, then needed minor (in-house)
maintenance - float sensors went bad. It will process up to 20" wide sheet,
in 90 secs. dry-dry, and the rollers stay submerged in the chemistry, which
seems to keep them cleaner (no dried on chemicals to deal with).

The Mohr has some rollers partially exposed, which often was a source of
roller marks, and were more difficult to clean. We used it primarily for
negatives (TEM & SEM). It performed well, just not as smoothly as the Ilford.

Overall, I had very good support from both Ilford and Mohr (though neither
produced problems that really tested the waters). I would recommend both,
but for different apps - Ilford for RC Prints (does not do film), and the
Mohr for negs. With the caveat that the Mohr will require more hands-on time.

JCL
=========================================================
James C. Long
Manager/Materials Analysis Lab
Electrosource, Inc.
512-445-6606
jlong-at-electrosource.com

----------------------------------------------------------






} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/





From: gwisler-at-asrr.arsusda.gov
Date: Thu, 21 Mar 1996 16:56:47 -0500 (EST)
Subject: Re: your mail

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Dear Sir: We have a dissassembled Siemens ELMISKOP 102 TEM which is
available free of charge for the taking. It was purchased in 1972, and
was working just fine when we moved it. We have a newer scope that we
like better. Please respond if you are interested.
G. C. Wisler
USDA-ARS
Salinas, Ca 93905





From: gwisler-at-asrr.arsusda.gov
Date: Thu, 21 Mar 1996 17:21:06 -0500 (EST)
Subject: Re: your mail

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Used TEM available:
1973 Siemens ELMISKOP 102. Was in fine working condition when it was
retired. Has been dissassembled and is in storage.
Interested persons contact:
Tom Nelson
USDA-ARS
1636 E. Alisal St.
Salinas, CA 93905




From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 21 Mar 1996 17:12:36 -0500 (EST)
Subject: Re: TEM safety

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} Just a short question. What about health risks for TEM users (and
} sample preparation) when expecting a baby?
}
Dear Francisca,
The MSA Technologists Forum put together a short book published
by San Francisco Press titled Electron Microscopy Safety Handbook, VC
Barber & J A Mascorro editors. I didn't see any referrence to pregnant
workers in the Table of Contents, but the book is very useful and inex-
pensive. I ordered my copy in 1994 from

San Francisco Press, Inc.
Box 426800
San Francisco CA 94142-6800
USA
as
Electron Microscopy Safety Handbook, 2nd edition
ISBN # 0-911302-72-7

For radiation, the standard for pregnant workers is an order of
magnitude lower than for other workers, and I'd apply the same reasoning
for chemical hazards, except that I'd be even more cautious for critical
periods of pregnancy when specific organ systems are developing. Congra-
tulations and good luck.
Yours,
Bill Tivol




From: zhiyu wang :      zhiyu-at-hawaii.edu
Date: Thu, 21 Mar 1996 08:44:54 -1000
Subject: Re: sectioning teeth

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X-Nupop-Charset: English

Hi, Jean:

I worked in a project dealt with ultrasection of fish otolith for several
years. My purpose was to get thin section in {50nm for EELS analysis.
Fish otolith is a piece of rock in fish's inner ear and very hard and
fragle. I have been succesfull in getting such ultrasection in Ultracut
E machine. Few technological points may help you to get better
sections.

Resin's hardeness can not macth the hardeness of otolith no matter what
recipes you use. Also the role of resin is for hold the sample instead
of embedding. When knife touch sample, the sample get a moment tending
to rotate, thus a gap between sample and resin will occur. Also sample
can not be cut due to the elastisity of resin but none of sample. After
few cutting cycles, the sample may be cut in thick section. My method
for these problems is as following:

After a few cutting, take block out of machine, put a drop of Spurr resin
on cutting surface (sample originally embedded in Epon resin)to fill out
the gap, put it in oven for polymerilization. Repeat above for several
times. This will have the hard sample to press its surounding of resin
and increse density of resine to have better holding result. Shortly, I
grind the block with #1200 sand paper instead of cutting. By this way, I
can get silve-gold color section in 0.2-0.5 mm squar of sample area.

Picking the sections is a difficult job. I use carbon film with copper
grid to pick the sections up. Sock the carbon film with 10%-30% alcohol
then touch the sections. Alcohol reduces water surface tension and
protect sections from damage. But only 10-30% chances can get good
sections on grid.


Hope this helps


Zhiyu Wang
Department of Biosystem Engineering
University of Hawaii at Manoa
Honolulu Hawaii
zhiyu-at-hawaii.edu




jean ross wrote:
}
} I have been working on a project with a dental grad student involving
} ultramicrotomy of undecalcified human teeth. The samples were prepared by
} slicing them into approx. 100um slices and then embedding them in Epon. They
} section just great but something happens when they're picked up on grids which
} causes just the dentin portion of the section to wrinkle. I use formvar coated
} 200 mesh copper grids, stabilized with carbon and glow discharged. I spread the
} sections out in the boat with chloroform (I have used xylene too) and have
} picked them up every differnt way I could think of. Is it my support film?
} Would a thicker section be desirable (sections are usually 90-95 nm)? It is
} only the dentin part of the section that has this problem, the Epon and
} composite resin reconstruction are perfectly flat. Does anybody have any
} suggestions?
}
} Thanks in advance,
}
} Jean Ross
} Central Microscopy Research Facility
} University of Iowa
} 85 EMRB
} Iowa City, IA 52242
} (319) 335-8142
} Web site: http://www.uiowa.edu/~cemrf




From: zhiyu wang :      zhiyu-at-hawaii.edu
Date: Thu, 21 Mar 1996 09:19:45 -1000
Subject: Re: sectioning teeth

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jean ross {jeanross-at-emiris.iaf.uiowa.edu}
Hi, Jean:

I worked in a project dealt with ultrasection of fish otolith for several
years. My purpose was to get thin section in {50nm for EELS analysis.
Fish otolith is a piece of rock in fish's inner ear and very hard and
fragle. I have been succesfull in getting such ultrasection in Ultracut
E machine. Few technological points may help you to get better
sections.

Resin's hardeness can not macth the hardeness of otolith no matter what
recipes you use. Also the role of resin is for hold the sample instead
of embedding. When knife touch sample, the sample get a moment tending
to rotate, thus a gap between sample and resin will occur. Also sample
can not be cut due to the elastisity of resin but none of sample. After
few cutting cycles, the sample may be cut in thick section. My method
for these problems is as following:

After a few cutting, take block out of machine, put a drop of Spurr resin
on cutting surface (sample originally embedded in Epon resin)to fill out
the gap, put it in oven for polymerilization. Repeat above for several
times. This will have the hard sample to press its surounding of resin
and increse density of resine to have better holding result. Shortly, I
grind the block with #1200 sand paper instead of cutting. By this way, I
can get silve-gold color section in 0.2-0.5 mm squar of sample area.

Picking the sections is a difficult job. I use carbon film with copper
grid to pick the sections up. Sock the carbon film with 10%-30% alcohol
then touch the sections. Alcohol reduces water surface tension and
protect sections from damage. But only 10-30% chances can get good
sections on grid.


Hope this helps


Zhiyu Wang
Department of Biosystem Engineering
University of Hawaii at Manoa
Honolulu Hawaii
zhiyu-at-hawaii.edu

jean ross wrote:
}
} I have been working on a project with a dental grad student involving
} ultramicrotomy of undecalcified human teeth. The samples were prepared by
} slicing them into approx. 100um slices and then embedding them in Epon. They
} section just great but something happens when they're picked up on grids which
} causes just the dentin portion of the section to wrinkle. I use formvar coated
} 200 mesh copper grids, stabilized with carbon and glow discharged. I spread the
} sections out in the boat with chloroform (I have used xylene too) and have
} picked them up every differnt way I could think of. Is it my support film?
} Would a thicker section be desirable (sections are usually 90-95 nm)? It is
} only the dentin part of the section that has this problem, the Epon and
} composite resin reconstruction are perfectly flat. Does anybody have any
} suggestions?
}
} Thanks in advance,
}
} Jean Ross
} Central Microscopy Research Facility
} University of Iowa
} 85 EMRB
} Iowa City, IA 52242
} (319) 335-8142
} Web site: http://www.uiowa.edu/~cemrf




From: zhiyu wang :      zhiyu-at-hawaii.edu
Date: Thu, 21 Mar 1996 08:43:22 -1000
Subject: Re: sectioning teeth

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Message-ID: {3151A34A.25E8-at-hawaii.edu}
jean ross {jeanross-at-emiris.iaf.uiowa.edu}
CC: Microscopy-at-Sparc5.Microscopy.Com

Hi, Jean:

I worked in a project dealt with ultrasection of fish otolith for several
years. My purpose was to get thin section in {50nm for EELS analysis.
Fish otolith is a piece of rock in fish's inner ear and very hard and
fragle. I have been succesfull in getting such ultrasection in Ultracut
E machine. Few technological points may help you to get better
sections.

Resin's hardeness can not macth the hardeness of otolith no matter what
recipes you use. Also the role of resin is for hold the sample instead
of embedding. When knife touch sample, the sample get a moment tending
to rotate, thus a gap between sample and resin will occur. Also sample
can not be cut due to the elastisity of resin but none of sample. After
few cutting cycles, the sample may be cut in thick section. My method
for these problems is as following:

After a few cutting, take block out of machine, put a drop of Spurr resin
on cutting surface (sample originally embedded in Epon resin)to fill out
the gap, put it in oven for polymerilization. Repeat above for several
times. This will have the hard sample to press its surounding of resin
and increse density of resine to have better holding result. Shortly, I
grind the block with #1200 sand paper instead of cutting. By this way, I
can get silve-gold color section in 0.2-0.5 mm squar of sample area.

Picking the sections is a difficult job. I use carbon film with copper
grid to pick the sections up. Sock the carbon film with 10%-30% alcohol
then touch the sections. Alcohol reduces water surface tension and
protect sections from damage. But only 10-30% chances can get good
sections on grid.


Hope this helps


Zhiyu Wang
Department of Biosystem Engineering
University of Hawaii at Manoa
Honolulu Hawaii
zhiyu-at-hawaii.edu



jean ross wrote:
}
} I have been working on a project with a dental grad student involving
} ultramicrotomy of undecalcified human teeth. The samples were prepared by
} slicing them into approx. 100um slices and then embedding them in Epon. They
} section just great but something happens when they're picked up on grids which
} causes just the dentin portion of the section to wrinkle. I use formvar coated
} 200 mesh copper grids, stabilized with carbon and glow discharged. I spread the
} sections out in the boat with chloroform (I have used xylene too) and have
} picked them up every differnt way I could think of. Is it my support film?
} Would a thicker section be desirable (sections are usually 90-95 nm)? It is
} only the dentin part of the section that has this problem, the Epon and
} composite resin reconstruction are perfectly flat. Does anybody have any
} suggestions?
}
} Thanks in advance,
}
} Jean Ross
} Central Microscopy Research Facility
} University of Iowa
} 85 EMRB
} Iowa City, IA 52242
} (319) 335-8142
} Web site: http://www.uiowa.edu/~cemrf




From: paulc-at-arms.gps.caltech.edu (Paul K. Carpenter)
Date: Thu, 21 Mar 1996 15:05:48 -0800
Subject: Re: EMPA garnet analysis

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Bob MacKay asked:

} A colleague and I were discussing microprobe analysis of garnets and
} he remarked that their analysis of Almandine often produce high totals,
} a phenomenon I have also observed with high Fe garnets. I know that
} others have observed this as well but has anyone come up with an
} explanation ?

Yes, the microprobe analysis of garnets is problematic. Actually, it is
silicate minerals of the Fe-Mg solid solution series in general that share
this. Here is a short summary of my understanding of the problem:

1. It is primarily the absorption correction component of the ZAF
algorithms that is responsible for over-correcting for x-ray absorption
thus leading typically to high totals in the analysis (i.e. for garnets
101-102, and for olivines more like 101% in the total). Here I am assuming
that end member oxide standards have been used (SiO2 for Si, MgO for Mg,
and Fe2O3 for Fe, for example). So when one uses these standards, the
analysis of a garnet or olivine yields a high total. The culprit may be
that the mass absorption coefficients are in error for Mg (in particular),
but also Si and Fe; these erroneous values lead us to the high totals due
to overcorrection using a "faulty" mac. John Donovan at Berkeley drew my
attention to this, by the way.

The problem in general is that we cannot simply adjust the mac values to
suit our needs in a particular compositional system because we may not
observe the same problem in a different system.

I don't remember the particulars, but it is also known that some factors in
the fluorescence algorithms were originally fudged to work for metals
(stainless steel), and that this optimization does not hold for silicate
systems.

So there are problems in several components of typical ZAF correction
schemes and the parameters they use. Both ZAF and Phi-rho-z schemes use
macs for absorption correction, by the way.

2. One really needs to use a standard as close in composition to the
sample to be analysed when dealing with Fe-Mg garnets and olivines. I get
good results for olivines using fayalite for Fe and an Fo90 olivine for Mg
and Si when analyzing olivines that are in the Fo70-90 range. Of course
all Fe can safely be assumed to be Fe2+ in these systems (but Fe3+ in
olivines is not unknown).

Garnets are a different story. I still observe high totals when using only
garnet standards to analyze garnet samples. This again points to mac
problems, but why we have success with olivines but not garnets is an
unknown. Garnets are fairly dense and so one wonders about density terms
in the equations; however, these terms cancel when the k-ratio is
calculated. Really well characterized end-member garnets can be hard to
find (like a pure almandine, for example).

I will just mention that even though the garnet stoichiometry seems
straightforward, that I have observed fluorine up to several thousand ppm
in grossulars, and have been told about hydrogrossular component as well.
It is possible, then, that the typical wet chemical analyses of our
standard grossulars are incomplete. Fe is present as both Fe2+ and Fe3+,
but one must be careful about making charge-balance vs. stoichiometric
assumtions to calculate Fe2/Fe3.

3. Calculation of oxygen by stoichiometry works pretty well. You should
try analyzing garnets for oxygen sometime (using garnet standards, for
example grossular), and you will see that the totals are *really* bad as a
result, compared to oxygen by stoichiometry. This points to the problems
in analyzing oxygen in general.

4. Note that garnets can exhibit chemical zoning, but that this zoning
results in the same average atomic number across this zoning due to coupled
substitution, so that backscattered-electron imaging is not as successful
at elucidating inhomogeneity as in other systems. This means that the
homogeneity of a garnet standard (natural material) is suspect until
verified by mapping or linescans.

And that is just what came to mind while I sat here for a few minutes!

Paul Carpenter


+------------------------------------------------------------+
| Paul K. Carpenter |
| Division Analytical Facility |
| Geological and Planetary Sciences MC 170-25 |
| California Institute of Technology |
| Pasadena, CA 91125 |
| 818-395-6126 (X-ray Lab) 818-568-0935 (FAX, Departmental) |
| paulc-at-arms.gps.caltech.edu |
+------------------------------------------------------------+






From: Zhiyu Wang :      zhiyu-at-hawaii.edu
Date: Thu, 21 Mar 1996 20:24:33 -1000
Subject: Re: sectioning teeth

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Microscopy-at-Sparc5.Microscopy.Com


I apology for posting message few times. I was not sure if the message
can be send via Netscape.


Zhiyu Wang








From: Bingqiang Lei :      Bingqiang.Lei-at-mb.luth.se
Date: Fri, 22 Mar 1996 09:06:36 +0800
Subject: who know?

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Message-Id: {3151FD1C.7163-at-mb.luth.se}

I need to by a computer software or databank which can deal with the some
problems we materials scientists usually encounter such as the mass diffusion,
thermal expansion and thermal conductivity. Please let me know if anybody has
such a software or knows something about that. I would appreciate any clue or
information to find those software.

Bingqiang Lei

-----------------------------------------------------
Name: Bingqiang Lei
Department: Materials and Manufacturing Engineering
University: Lulea University of Technology
Tel: (46) 920 91233
fax: (46) 920 99309
Email: Bingqiang.Lei-at-mb.luth.se
-----------------------------------------------------




From: rgwhite-at-vaxc.cc.monash.edu.au (Rosemary White)
Date: Fri, 22 Mar 1996 18:06:10 +1200
Subject: Re: TEM:LRW/Eppendorfs revisited

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Re:

} } Thanks to everyone who responded to my call for help. In case anyone is
} } interested, the replies split about 70/30 in favor of Eppendorfs, but
} } several people said they'd never been able to get them to work for LRW.
} [snip]
} Just wanted to point out that the term "Eppendorf tube" is a bit
} like "whiskey", understood instantly, but in fact refering to a wide range
} of product. So, "ep tubes" are made by gazillions of companies and
} presumably out of, well, dozens, of different kinds of plastics. Might be
} worth finding out from someone in the successful camp exactly where they
} bought their ep tubes.
}

This is a good point. For consistently good results, we use "real"
Eppendorf tubes, i.e. made by Eppendorf. We did have problems with
slightly cheaper ones once, though I've forgotten exactly what the trouble
was.

Rosemary White
__ /
Department of Ecology _/ \__/ \
and Evolutionary Biology / \
Monash University / Australia \
Clayton, Victoria 3168 \ ____ /
phone 61-3-9905 5670 \_/ \_*_/
fax 61-3-9905 5613 __
email rgwhite-at-vaxc.cc.monash.edu.au \/






From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 21 Mar 1996 17:12:36 -0500 (EST)
Subject: Re: TEM safety

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From: Stephan Helfer :      S.Helfer-at-rbge.org.uk
Date: Fri, 22 Mar 1996 11:09:21 BST
Subject: Re: TEM baby

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Dear Francisca
When our assistant got pregnant I insisted in her leaving all work
involving hazardous chemicals to other people (mainly me). The
operation of modern EMs should not be hazardous. Basically, the
pregnant operator should feel confident that she and her baby are not
at exposed to additional hazards.
The risks of something going wrong and the TEM getting the blame
(rightly or wrongly) are not worth taking.

Yours sincerely

Dr Stephan Helfer, SSO
Mycologist / Plant Pathologist

Royal Botanic Garden Edinburgh, Inverleith Row, EDINBURGH EH3 5LR,
Scotland UK

http://www.rbge.org.uk

phone: +44 (0)131 552 7171 ext 280
or +44 (0)131 459 0446-280 (direct digital VoiceMail line)
fax: +44 (0)131 552 0382
============================
1896
* BRITISH
* MYCOLOGICAL
* SOCIETY
1996
A century of fungal science
============================




From: WARRENJ1-at-cliffy.polaroid.com
Date: Thu, 21 Mar 1996 22:08 -0400 (EDT)
Subject: NIH Image

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Where can I download a copy of NIH Image?

Thanks

John Warren
Helios Scientific Group
Polaroid Corporation




From: Kingsley H. McCrocklin :      kingsley-at-zephyr.nrlssc.navy.mil
Date: 3/22/96
Subject: nanoplast/melamine resin

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This message was sent by Chameleon
-------------------------------------





From: jean ross :      jeanross-at-emiris.iaf.uiowa.edu
Date: Fri, 22 Mar 1996 07:37:16 -0600 (CST)
Subject: Re: Variable pressure SEM: fresh plant tissue

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We have an S-2460N without a cold stage and have gotten great results at 25Pa
and 18Kv with about 60uA of emission. The cold stage will make a diference but
maybe this will give you a place to start.

Jean Ross
Central Microscopy Research Facility
Univ. of Iowa
(319) 335-8142
web site: http://www.uiowa.edu/~cemrf


On Thu, 21 Mar 1996, HENRY P ADAMS wrote:

} We recently acquired a variable pressure sem (Hitachi S3200N) with a Fullam
} cold-stage, a researcher wants to image fresh leaves and is particularily
} interested in the status (open vs closed) of the stomates. Does anyone
} have any suggestions as to temp, pressure, Kv, beam current, etc?
}
} TIA,
} Hank Adam
}






From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Fri, 22 Mar 1996 09:44:13 -0600
Subject: Re: Variable pressure SEM: fresh plant tissue

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LIST-at-itasca.cems.umn.edu, Microscopy-at-Sparc5.Microscopy.Com

Responding to message {Pine.SUN.3.91.960321120104.6864B-100000-at-verdi}
from HENRY P ADAMS {hadams-at-nmsu.edu} :
}
} We recently acquired a variable pressure sem (Hitachi S3200N) with a
} Fullam
} cold-stage, a researcher wants to image fresh leaves and is particularily
}
} interested in the status (open vs closed) of the stomates. Does anyone
} have any suggestions as to temp, pressure, Kv, beam current, etc?
}
} TIA,
} Hank Adam
}
On our Electroscan environmental SEM we look at plant tissue at a few
degrees above freezing. This allows us to keep them hydrated at a pressure
of 5-6 torr water vapor in the chamber. At 0 Celcius the saturated vapor
pressure of water is about 4 torr, so at pressures lower than this the
material dehydrates very quickly. We typically use fairly low kV (5-10kV)
to see more surface detail. Under these conditions the plant material can
survive for reasonably long periods of time.

Stuart McKernan
University of Minnesota Characterization Facility






From: smiller-at-umr.edu (Scott Miller)
Date: Fri, 22 Mar 1996 12:01:14 -0600
Subject: WTB:Goniometer tools for Philips EM400 TEM

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I would like to ask if anyone has the goniometer tool kit for a Philips 400
series TEM that they would be willing to sell. I would prefer to hear from
someone in the US, but all offers will be considered.

F. Scott Miller
Electron Microscope Lab smiller-at-umr.edu
University of Missouri-Rolla voice: 314 341 4727
Rolla, MO 65401 USA fax: 314 341 6934








From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Fri, 22 Mar 1996 08:51:58 GMT
Subject: Re: TEM:LRW/Eppendorfs revisited

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} Greetings,
} Tamara wrote:
}
} } Thanks to everyone who responded to my call for help. In case anyone is
} } interested, the replies split about 70/30 in favor of Eppendorfs, but
} } several people said they'd never been able to get them to work for LRW.
} [snip]
} Just wanted to point out that the term "Eppendorf tube" is a bit
} like "whiskey", .........


What I should have said was polypropylene Eppendorf-type tubes work fine.
Fisher Scientific and USA Scientific make the ones we use. We use doggie
toenail clippers to snip off the end of the tube and then tease or cut out
the polymerized sample and remount it for trimming and sectioning. If the
whole embedding process takes place in the tube, take care to see that there
is good exchange of fluids at the bottom of the tube or else you could have
trouble.

I have never drunk any whiskey from them however.

-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: kna101-at-utdallas.edu
Date: Fri, 22 Mar 1996 07:57:55 -0600 (CST)
Subject: Re: your mail

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Francisca:
The lab I work in now rents time on someone else's scope, so I
haven't asked about extra saftey precautions they take, but I used to
work in a lab with it's own TEM. Our scope was checked yearly by
environmental health and saftey to make sure that the sheilding the scope
comes with was still doing it's job. It always was, but when we had a
pregnant woman using the lab., she wore a lead apron whenever she had to
be in the scope room. She also was carefull to wear gloves when handeling
chemicals, and use a fume hood. She ended up with a very healthy, rather
big, (8 pounds, plus) baby boy. She may have taken more precaution than
she needed to but it was better than not being careful enough. Good luck.
Karen Pawlowski

On Thu, 21 Mar 1996, Francisca Peiro wrote:

} Hi all,
} Just a short question. What about health risks for TEM users (and
} sample preparation) when expecting a baby?
}
} Thank you for your suggestions.
}
} F. Peiro
} =====================================================================
} Enginyeria i Materials Electronics Tel. (34-3) 402-11-39
} (34-3) 402-11-47
} Dep. Fisica Aplicada i Electronica FAX. (34-3) 402-11-48
} Universitat de Barcelona e-mail:paqui-at-iris1.fae.ub.es
} Avg. Diagonal 645-647
} 08028 Barcelona, Catalunya
} Spain
} =====================================================================
}
}




From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 22 Mar 1996 12:52:29 -0500 (EST)
Subject: Re[3]: TEM safety

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} } Just a short question. What about health risks for TEM users (and
} } sample preparation) when expecting a baby?
} }

[snip]

} Is the same true for SEM and FESEM (field emission SEM)?
} - JESS MUNOZ

Dear Jess,
The chemical hazards pertain to the preparation methods and are
independent of the type of instrument (except as the prep methods are mo-
dified for a particular instrument). The radiation hazard arises from
brehmsstrahlung x-rays generated by the interaction of the stray beam
electrons with apertures, the lens column and anything else that electrons
can strike. The amount of potential hazard is dependent on both voltage
and current, but the standards for radiation exposure are the same regard-
less of instrument type. As another contributor to this thread said, mod-
ern EMs are well designed for minimizing exposure and are pretty safe. If
there is any concern, a personnel monitoring device, such as a film badge
or thermoluminescent detector can be placed at the scope for a month, and
the radiation exposure can be monitored, and/or a survey meter can be used
to get a real-time readout of the radiation field.
Yours,
Bill Tivol




From: jdonovan-at-garnet.berkeley.edu (John J. Donovan)
Date: Fri, 22 Mar 1996 17:40:04 -0700
Subject: subscribe

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===============================================================================
John J. Donovan (510) 642-5459 (phone)
Room 301, McCone Hall (510) 643-9980 (FAX)
Department of Geology and Geophysics jdonovan-at-seismo.berkeley.edu
University of California jdonovan-at-garnet.berkeley.edu
Berkeley, CA
94720-4767
===============================================================================






From: karenw-at-ucmp1.berkeley.edu (Karen Wetmore)
Date: Fri, 22 Mar 1996 11:46:41 -0700
Subject: Re: Variable pressure SEM: fresh plant tissue

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I recently helped some plant biologists obtain images of pieces of maize
leaves with the stomata partially open. We used our ElectroScan ESEM with
ElectroScan's Peltier stage (a cooling stage). The images were taken at
5kV with the stage temperature set to about 5.5 degrees C. We increased
the chamber pressure to condense water on the leaf (to about 6 Torr as I
recall), then reduced the chamber pressure to slowly evaporate the water
enough to see the leaf and capture an image. The one image I saved for my
image gallery was taken at 5.3Torr. You can see it at our web site:

http://ucmp1.Berkeley.EDU/esem/gallery.htm

I have done similar condensation/evaporation experiments on other subjects
using different stage temperatures and chamber pressures, mostly around 5C
and 5Torr. The size of the subject and how closely it conforms to the stub
surface (we have cup-shaped as well as flat stubs for the Peltier) all make
a difference. The above settings will give you a starting point for
experimenting to determine what works best for your setup.

Karen Wetmore

} We recently acquired a variable pressure sem (Hitachi S3200N) with a Fullam
} cold-stage, a researcher wants to image fresh leaves and is particularily
} interested in the status (open vs closed) of the stomates. Does anyone
} have any suggestions as to temp, pressure, Kv, beam current, etc?
}
} TIA,
} Hank Adam

*****************************************************************
Karen L. Wetmore, Ph.D.
Museum Scientist
Museum of Paleontology
1101 VLSB #4780
(510) 642-0203
University of California
fax (510) 642-1822
Berkeley, CA 94720-4780 karenw-at-ucmp1.berkeley.edu
*****************************************************************






From: jdonovan-at-garnet.berkeley.edu (John J. Donovan)
Date: Fri, 22 Mar 1996 16:56:09 -0700
Subject: Re: EMPA garnet analysis

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} A colleague and I were discussing microprobe analysis of garnets and
} he remarked that their analysis of Almandine often produce high totals,
} a phenomenon I have also observed with high Fe garnets. I know that
} others have observed this as well but has anyone come up with an
} explanation ?

The problems with high totals in the Si-Mg-Fe system seem to be caused by a
bad mass absorption coefficient utilized by most programs of Mg Ka absorbed
by Fe. Most software I have seen use a value tabulated (but not measured)
by Heinrich. This use of Heinrich's tabulation is apparently part of the
the cause of high totals (when extrapolating from pure oxide or end-member
compositions) for many minerals, especially those with high Mg-Fe
concentrations, such olivine and garnets.

Please note the following values (soft x-ray) quoted from Heinrich and Henke :

Heinrich Henke (1982)


Mg Ka in Si 802 859
Mg Ka in Fe 6121 5250
Si Ka in Mg 2825 2902
Si Ka in Fe 2502 2305

As you can see there is about 20% difference in the mass absorption
coefficients
for Mg ka in Fe, although the others are reasonably close. This difference
will have a significant effect on the quantitative analysis (about 1 % or
so).

I have integrated the Henke value into my software as a default table and
used pure end-member olivines as standards for garnet analyses and have not
seen problems with high totals. There may be more going on here than just a
bad MAC or two, but since this is the largest correction we make to our
data, it's worth looking at first.

john

===============================================================================
John J. Donovan (510) 642-5459 (phone)
Room 301, McCone Hall (510) 643-9980 (FAX)
Department of Geology and Geophysics jdonovan-at-seismo.berkeley.edu
University of California jdonovan-at-garnet.berkeley.edu
Berkeley, CA
94720-4767
===============================================================================






From: SALLY STOWE :      stowe-at-rsbs-central.anu.edu.au
Date: Sat, 23 Mar 1996 17:24:33 EST10
Subject: Re: Variable pressure SEM: fresh plant tissue

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} We recently acquired a variable pressure sem (Hitachi S3200N) with a Fullam
} cold-stage, a researcher wants to image fresh leaves and is particularily
} interested in the status (open vs closed) of the stomates. Does anyone
} have any suggestions as to temp, pressure, Kv, beam current, etc?
}
} TIA,
} Hank Adam
}
We use a Hitachi 2250N, also with a Fullam cold-stage for the
moment. . We set to around -15-20 degrees if possible to allow
for a temperature gradient through the leaf, and work usually at
around 0.1-0.3 torr, adjusting beam current to the minimum
convenient. However we generally fast-freeze the leaves in liquid
nitrogen before transferring to the SEM stage, to try to minimize
any change in stomatal state during the transition. An alternative
is to set the frozen leaf on a block of metal (preferably steel)
cooled in LN2 - this allows 30 minutes or more of observation
before drying artifacts set in.

Sally Stowe

----------------------------------------------------------------------
Sally Stowe |Email: stowe-at-rsbs.anu.edu.au
Facility Coordinator |Post:
ANU Electron Microscopy Unit |ANUEMU (RSBS)
Ph 61 6 249 2743 |Australian National Univ.
FAX 61 6 249 4891 |Canberra,
http://online.anu.edu.au/EMU/home.htm |AUSTRALIA 0200






From: cal-at-ssnet.com (cal Montgomery)
Date: Sat, 23 Mar 1996 09:14:12 -0500
Subject: Used TEM for sale

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Am posting this for a someone who doos not have email at the moment.

They have a fully functioning Hitachi H-600 TEM with scanning attachment
available at a reasonable price. Also have available a ultramicrotome,
knifemaker, plus.

If interested can either email back to me at cal-at-ssnet.com or call them
directly at M.H. Systems at 419-647-6400.

Instrument is still set up and running.

Thank You,

Cal
Cal Montgomery





From: Doug Keene :      DRK-at-SHCC.ORG
Date: Sat, 23 Mar 1996 08:53:28 -0800 (PST)
Subject: unsubscribe

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unsubscribe please




From: Dr. L. P. Stoter :      LPS-at-teknesis.demon.co.uk
Date: Sat, 23 Mar 1996 16:41:58 +0000
Subject: Subscribe microscopy

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Subscribe microscopy

--------------------------------------------------------------
Dr. Larry Stoter
Technesis
17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, United Kingdom
Larry-at-teknesis.demon.co.uk
--------------------------------------------------------------






From: Jill Craig :      jcraig-at-unbc.edu
Date: Sat, 23 Mar 1996 18:28:21 -0800 (PST)
Subject: Unions

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Thanks for all the input regarding unions!

In summary:

Individuals working in the UK are generally unionized and are satisfied
with what unions do for them.

In the US, most are not unionized
although some would like to be or at least see points in favor of them. Some
have had the option although most have declined it. It appears that in the
US you can choose whether to be in the union or not on an individual basis.

In Canada, most are unionized although the attitude toward the union
ranges from abivalent through sceptical and resigned to decidedly anti.


Thanks for the info.

Jill




From: rh208-at-cus.cam.ac.uk (Ray Hicks)
Date: Mon, 25 Mar 1996 10:08:14 +0000
Subject: Re: Oil Sample

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Hi,
Further to the following, wouldn't a low-tech solution work for identifying
the contaminant/s? It should be quite easy to tell if it's sodium (solid,
reacts with water to give sodium hydroxide) or mercury (dense metallic
liquid). By mixing the oil with water and shaking for a while some of the
putative sodium would react, and you could assay the aquatic phase for
NaOH. By mixing the oil with a thinning solvent, or just by centrifuging,
you'd be able to separate mercury. If neither of these yield positive
results you're left with the third option (oil). Speak to a local
inorganic chemist, or get a book out of the library on small-scale
inorganic qualitative analysis to check out the feasibility of the above.


Ray


} I would filter the sample trough a membrane filter (Millipore?) and wash
} with a suitable solvent (n=hexane?) to eliminate the oil. Then dry and
} carbom coat.
} The residue in the membrane should be easily observed in SEM.
} Dagoberto Rodriguez
} -------------------------
}
} } I have an Amray 1600 Turbo SEM, I have a question about a sample
} }
} } that I would like to observe. The sample is an oil that a plant
} }
} } uses for a dosing machine. There are visible spots in the oil that we
} }
} } suspect may be Hg or Na, or the oil may be a synthetic oil, one that was
} }
} } not supposed to be used in the first place. I was asked if it is possible
} }
} } to examine a smear of the oil under SEM/EDX and identify the spots in it,
} }
} } but I'm thinking no because of the wet oil contaminating my sample chamber,
} }
} } and also the temperature of the electron beam may volatile the mercury.
} }
} } Is it not possible to observe this oil with my instrument? Are there any
} }
} } suggestions that I may follow?
} }
} }
} } Mark Darus
} } General Electric Co.
} }
} } Darus-at-cle.dnet.ge.com
} }
} }

Ray Hicks
________________________________________________________________________
|University of Cambridge |Tel 01223 330149 |
|Department of Medicine |Fax 01223 336846 |
|Level 5, Addenbrookes Hospital |e-mail rh208-at-cus.cam.ac.uk |
|Hills Road Cambridge |Web Page/ facsmac.med.cam.ac.uk |
|CB2 |ftp server 131.111.80.78 |
|UK | |
|_________________________________|_____________________________________|






From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 25 Mar 1996 10:07:51 +0100
Subject: answers to: need books on biology...

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Here is a digest of answers to my request of titles of books on biology
laboratory activities. Some people asked me to send a synthesis of responses.
I would like to thank very much all who have contributed to this list.
Bye george
giocar-at-risc990.bologna.enea.it
www.best.com/~funsci (amateur scientist site)

---------------------------------------------------------------



but a good microscopy techniques book is "Electron Microscopy:
Principles and Techniques for Biologists" by John J. Bozzola and
Lonnie D. Russell, editors. Jones and Bartlett Publishers, Boston.
1992.












--------------------------------------------------------------------
- the followings are answers to the request about "LM: lab manuals, textbooks"

Best books I know of are John Kiernan's _Histological and
Histochemical Methods_ and the Biological Stain Commission's _Staining
Procedures_ (9th or 10th [or...?] edition). I don't have Kiernan's book to
hand, but if he doesn't respond, I can send you the correct info. (Same for
_Staining Procedures_.) I heard there was a good EM book with some
procedures written by some guy in southern Illinois...


--------------------------------------------------------------
- I know this book:

Dennis E. Ohman, Experiments in gene manipulation, Prentice Hall Inc. 1988





From: Gary Login :      glogin-at-bih.harvard.edu
Date: Mon, 25 Mar 1996 10:59:07 -0500
Subject: Re: Microwave in EM

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In message {Pine.LNX.3.91.960325134158.3078A-100000-at-limax.paru.cas.cz}
Nebesarova LEM Motejl writes:
} I would ask somebody for an advice how to measure by simple way the
} temperature in a specimen in a domestic microwave oven and if it's better
} to remove quickly the animal tissue specimen from the warm fixation
} solution after the microwave exposure or to leave it in for long time there.
} Many thanks in advance.
} Jana Nebesarova
} Laboratory of Electron Microscopy
} Institute of Parasitology
} Ceske Budejovice
} Czech Republic


Dear Jana: your questions are very important with respect to achieving
reproducible microwave fixation.
1. I recommend a device called a 'Fix-N-Temp' container for simple, rapid,
reliable, and inexpensive temperature measurement of specimens fixed in a
microwave oven for EM studies .

a. You can make a 'Fix-N-Temp' container by sticking a liquid crystal
temperature strip into the bottom half of a 35 mm diameter plastic tissue
culture dish (Beckton Dickinson, Lincoln Park, NJ, USA) along the inside
diameter of the dish. I recommend using the liquid crystal temperature strip
available from Owl Scientific Plastics, Woburn, MA, USA (it measures
temperatures between 35 C and 65 C- ideal for the temperature range for
microwave fixation). Alternatively, this device can be purchased ready made for
several dollars from some EM Supply companies.

b. Place your fixative (up to 5 ml) in the dish, place your tissue (1 mm3 up to
1 cm3) in the fixative and irradiate according to your fixation protocol. You
will see the temperature of the solution displayed on the strip as soon as you
remove the dish from the microwave oven. The temperature is accurate to within
5 C. The container and liquid crystal strip are reusable.

c. Other methods for measuring temperature require the use of electronic
temperature probes which are more expensive, have a slower response time, and
are in my experience less accurate. In addition, the temperature probes in
microwave ovens are known to distort the microwave fields and cause conductive
heating artifacts if placed near a tissue sample.

2. The tissue should be removed from the warmed fixative as soon as microwave
irradiation has stopped. Tissues left in warm fixative are damaged by
conductive heating from the solution. Specimens up to several cubic mm can be
fixed in less than 30 seconds in a microwave oven that has been properly
calibrated.

These issues are discussed in detail in the following two references:
1. Login GR, Dvorak AM. Methods of microwave fixation for microscopy. A review
of research and clinical applications: 1970-1992. Prog Histochem Cytochem
1994;27/4: 1-127.

2. Login GR, Dvorak AM. The Microwave Toolbook. A Practical Guide for
Microscopists (Beth Israel Hospital, Boston, 1994).

Please contact me if you would like additional information.




Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676





From: petford-at-vax.ox.ac.uk
Date: Mon, 25 Mar 1996 09:22:19 +0000
Subject: TEM safety

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Dear Francisca, I work in the Materials Department at the University of Oxford,
and carry out a lot of TEM work, especially using 400kV JEOL machines. I have
had two pregnancies whilts using the TEMs, as has another woman in my
department, all with absolutely no problems at all. We had the Radiation
Protection Office check our machines for radiation levels and they all came way
below any danger levels. The only difficulty comes when you get too big to
reach the console!!

Amanda Petford-Long.




From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Mon, 25 Mar 1996 10:48:28 +1100
Subject: Re: NIH Image

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} Where can I download a copy of NIH Image?


NIH Image home page is at http://rsb.info.nih.gov/nih-image/. From here you
download the programme (current is 1.60b7) and manual.

Diana van Driel
Dept Ophthalmology
Sydney University
AUSTRALIA 2006






From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Mon, 25 Mar 1996 10:12:30 +1100
Subject: Re: TEM baby

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When I was pregnant I did almost no lab work involving hazardous chemicals
(fixing, embedding and photography particulary). I cut sections, but was
careful of the resin (no sawing blocks in half). I kept EM time to a
minimum. There are regulations in Aust. stipulating what pregnant women can
be exposed to and the concentrations are MUCH lower than for general
workers, but basically steer clear of the lot if you can. Babies and the
adults they grow into are a lot more important than missing out on
processing a few specimens - someone else can do it if it's important. If
the powers that be don't agree, stand up for your babies rights and don't
give in.

Diana van Driel
Dept Ophthalmology
Sydney University
AUSTRALIA 2006






From: Len Adleman :      adleman-at-pollux.usc.edu
Date: Sun, 24 Mar 1996 12:36:21 -0800 (PST)
Subject: Please subscribe

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I would like to subscribe:
adleman-at-cs.usc.edu
(I am unsure that this group is appropriate for my needs so
it is possible I will unsubscribe after a short trial)
Thanks




From: yimei-at-befvax.uchicago.edu
Date: Mon, 25 Mar 1996 11:50:05 EST
Subject: Subscribe

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From: James S MArtin :      James.S.Martin-at-williams.edu
Date: Mon, 25 Mar 1996 12:50:31 -0500 (EST)
Subject: Re: EDS atlas of materials

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Jesus:

The Particle Atlas is an invaluable resource for particle analysts.
Volumes 3 and 6 may contain SE micrographs and EDS spectra of use to
you. The Particle Atlas was originally printed as a six-volume hardbound
edition:

The Particle Atlas. ed. Walter McCrone. Ann Arbor, MI: Ann Arbor Science
Publishers, Inc.

The Particle Atlas is also available on CD-ROM from MicroDataware
(800/582-6624). See a review by Walter McCrone:

McCrone, W. "The Particle Atlas, Electronic Edition," American Laboratory
(April 1993): 39-44.


James Martin
Director of Analytical Services and Research
Williamstown Art Conservation Center

standard disclaimer of financial interest


On Mon, 25 Mar 1996, Jesus Munoz wrote:
n
}
} Hi all.
}
} I'm currently doing EDS (Energy Dispersive Spectroscopy, some call it
} EDX) analysis of contaminants caught in our production line. As you
} know, EDS gives the composition of a substance under analysis. But
} what's more important is for us to know/identify what the material is
} and where it came from (ex. Fe, Ni, Cr is stainless steel, Powder has
} Si & Mg).
}
} Is there a book, magazine, atlas, or anything that could give me such
} information? I'd appreciate any help you could give. Thanks..
}
} JESS MUNOZ
}
}






From: Dave King (607)857-1248 T37/257-3 Ext DEKING-at-VNET.IBM.COM
Date: 25 Mar 1996 12:46:35 EST
Subject: EDS atlas of materials

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Message-Id: {199603251744.LAA02166-at-Sparc5.Microscopy.Com}

To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}
cc: VANHART --ENDVM5 Dan VanHart 7-1262
*** Reply to note of 03/25/96 09:23

McCrone's Particle Atlas is great for materials source ID info.
The McCrone Institute is in Chicago. They have a home page at;

http://www.mcri.org

{
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: Joseph P. Neilly 708-938-5024 :      NEILLY.JOSEPH-at-igate.pprd.abbott.com
Date: Mon, 25 Mar 1996 13:57:00 -0600 (CST)
Subject: Re:EDS atlas of materials

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Mr-Received: by mta RANDD; Relayed; Mon, 25 Mar 1996 14:08:11 -0600
Mr-Received: by mta MCM$RAND; Relayed; Mon, 25 Mar 1996 14:08:13 -0600
Mr-Received: by mta RANDB; Relayed; Mon, 25 Mar 1996 14:08:21 -0600
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Jess,

You could try the McCrone Particle Atlas. It is no longer in print but
an electronic copy is available on CD-ROM from:

MicroDataWare
2894 Tribune Ave
Hayward, CA 94542
Phone: 1-800-582-6624

The atlas contains microscopic data from a variety of materials
including optical micrographs, SEM and EDS.

Good Luck,

Joe Neilly
Abbott Laboratories
North CHicago, IL






From: Nebesarova LEM Motejl :      lem-at-paru.cas.cz
Date: Mon, 25 Mar 1996 13:53:10 +0100 (GMT+0100)
Subject: Microwave in EM

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I would ask somebody for an advice how to measure by simple way the
temperature in a specimen in a domestic microwave oven and if it's better
to remove quickly the animal tissue specimen from the warm fixation
solution after the microwave exposure or to leave it in for long time there.
Many thanks in advance.
Jana Nebesarova
Laboratory of Electron Microscopy
Institute of Parasitology
Ceske Budejovice
Czech Republic




From: Douglas F Bowling :      al428-at-dayton.wright.edu
Date: Mon, 25 Mar 1996 15:43:19 -0500
Subject: Subscribe

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Subscribe




From: SveEn-at-pai.liu.se (Sverker Enestrom)
Date: Mon, 25 Mar 1996 11:12:26 +0200
Subject: Re: Stains

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Bob:
Why not simply try Periodic acid-Schiff? Periodic acid will cleave the glyceryl
C-C bonds adjacent to -OH groups; the aldehydes are then demonstrated with
Schiff.
Sverker

} Does anyone know of a stain that is specific to either carbonyl or hydroxyl
} groups? If possible, I would like to find a way to visibly stain gylceryl
} monostearate on the surface of polypropylene.


*********************************************************
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
Fax: +46 13 13 22 57
*********************************************************






From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Mon, 25 Mar 1996 07:33:00 -0300 (EST)
Subject: LM: diamond knife for resin sections

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I want to buy a 8 mm diamond knife to cut material
embedded in historesin, but I would like some
information about it.
Does anyone have any experience with this material?
Who sells it and how much does it cost?
I will be very grateful for any help.

_____________________________________________________________________________
Francisco Javier Hernandez Blazquez * Av. Prof. Lineu Prestes, 1524
Departamento de Histologia e Embriologia * 05508-900 Sao Paulo
Instituto de Ciencias Biomedicas * e-mail fjhblazq-at-spider.usp.br
Universidade de Sao Paulo * fjhblasq-at-biomed.icb2.usp.br
______________________________________________________________________________






From: Jeffrey.Shield-at-mse.utah.edu (Jeff Shield)
Date: Mon, 25 Mar 1996 14:26:18 -0700 (MST)
Subject: Focused Ion Beam Analysis

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Greetings,

A colleague is needing compositional analysis from 10 nm layers. He is
interested in doing focused ion beam analysis considering sample prep
difficulties with TEM. Since I am not familiar with FIB, is this a viable
technique for his problem? If it is, is there anyone out there willing to
analyze his samples?

Jeff Shield





From: pwkr-at-ugcs.caltech.edu (paul wilhelm karl rothemund)
Date: Mon, 25 Mar 1996 15:15:05 -0800 (PST)
Subject: subscribe

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I would like to subscribe:
rothemun-at-pollux.usc.edu

Thanks, Paul Rothemund




From: HENRY P ADAMS :      hadams-at-nmsu.edu
Date: Mon, 25 Mar 1996 13:54:02 -0700 (MST)
Subject: Re: EDS atlas of materials

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On Mon, 25 Mar 1996, Jesus Munoz wrote:

}
} Hi all.
}
} I'm currently doing EDS (Energy Dispersive Spectroscopy, some call it
} EDX) analysis of contaminants caught in our production line. As you
} know, EDS gives the composition of a substance under analysis. But
} what's more important is for us to know/identify what the material is
} and where it came from (ex. Fe, Ni, Cr is stainless steel, Powder has
} Si & Mg).
}
} Is there a book, magazine, atlas, or anything that could give me such
} information? I'd appreciate any help you could give. Thanks..
}
} JESS MUNOZ
}
There is an atlas authored by Walter C. McCrone and John G. Delly, titled
the The PARTICLE ATLAS, a 4 volume set, however, vol.IV contains edx
spectra and SEM of particles. The other volumes deal with other aspects
of particle identification such as princibles and techniques, polarizing
microscopy, etc. They are all useful. The set we have in our lab is 2nd
ed, copyright 1973 by Ann Arbor Science Publishers, Inc., Ann Arbor,
Michigan, USA. ISBN # 0-250-40008-1. I don't know if there is a new
edition out, Walter McCrone is the father of particle identification.
Good luck, Hank Adams
}




From: Scott Williams :      scott_williams-at-pch.gc.ca
Date: 3/25/96 6:15 PM
Subject: EDS atlas of materials

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Alternate-Recipient: prohibited
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Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Normal



Hi all.

I'm currently doing EDS (Energy Dispersive Spectroscopy, some call it
EDX) analysis of contaminants caught in our production line. As you
know, EDS gives the composition of a substance under analysis. But
what's more important is for us to know/identify what the material is
and where it came from (ex. Fe, Ni, Cr is stainless steel, Powder has
Si & Mg).

Is there a book, magazine, atlas, or anything that could give me such
information? I'd appreciate any help you could give. Thanks..

JESS MUNOZ


The following is an attached File item from cc:Mail. It contains
information that had to be encoded to ensure successful transmission
through various mail systems. To decode the file use the UUDECODE
program.
--------------------------------- Cut Here ---------------------------------
begin 644 RFC822.TXT



From: Scott Williams :      scott_williams-at-pch.gc.ca
Date: 3/25/96 6:15 PM
Subject: EDS atlas of materials

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end





From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 25 Mar 1996 14:21:34 -0800 (PST)
Subject: Re: LM: diamond knife for resin sections

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Hello, We have been using the Diatome Histo-Diamond knives for several
years to cut Historesin and epoxies. These knives are very durable and a
real timesaver for extensive serial sectioning and for cutting things that
savage glass knives, like a little bone or otoconia. They've survived
sectioning up to 5 microns and cut well down to 0.5. At 0.5 microns the
sections start to look a little scratchy. They won't section a lot of
bone, someone here made an unauthorized attempt to section an untrimmed
undecalcified cochlea. The knive sectioned with lots of scratch marks
afterwards, but cut well after being sent back for resharpening.

You will want to clean the knive periodically with mild detergent for
optimal performance.

Regards,

Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu


On Mon, 25 Mar 1996, Francisco J. Hernandez wrote:

}
} I want to buy a 8 mm diamond knife to cut material
} embedded in historesin, but I would like some
} information about it.
} Does anyone have any experience with this material?
} Who sells it and how much does it cost?
} I will be very grateful for any help.
}
} _____________________________________________________________________________
} Francisco Javier Hernandez Blazquez * Av. Prof. Lineu Prestes, 1524
} Departamento de Histologia e Embriologia * 05508-900 Sao Paulo
} Instituto de Ciencias Biomedicas * e-mail fjhblazq-at-spider.usp.br
} Universidade de Sao Paulo * fjhblasq-at-biomed.icb2.usp.br
} ______________________________________________________________________________
}
}
}





From: scott_l-at-unin1.unorth.ac.za
Date: Tue, 26 Mar 1996 12:28:02 -0200
Subject: Stereology

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Message-Id: {199603260425.XAA00633-at-use.usit.net}

Are there IBM compatible PC based programmes able to assist with doing
stereology from TEM and LM micrographs. What are the hardware require-
ments?

Thank you
Leon Scott
SA




From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Tue, 26 Mar 1996 08:38:11 GMT
Subject: Re: Stereology

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At 12:28 PM 3/26/96 -0200, you wrote:
} Are there IBM compatible PC based programmes able to assist with doing
} stereology from TEM and LM micrographs. What are the hardware require-
} ments?
}
} Thank you
} Leon Scott
} SA
}
}
You will find an archived discussion about the presentation
of stereo pairs on the web page listed at the bottom of this message. Look
in the "Tips & Tricks" section. There was some discussion about software. I
will be more than happy to e-mail the file to you as well if you are unable
to access the page.
Cheers




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/





From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Tue, 26 Mar 1996 08:54:37 -0500 (EST)
Subject: actin

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Fellow Microscopists:

I am starting to look at tapeworms in the Trypanorynch group and was
wondering if there is a suggestion for a good fix for the preservation of
"actin"? I normally use 2.5% glut in cacodylate buffer. Will this be
O.K. or is there something better? I greatly appreciate any help.

Peace,

Phil Rutledge
8-{)




From: Elinor Solit :      cambrex-at-world.std.com
Date: Tue, 26 Mar 1996 10:48:42 -0500 (EST)
Subject: Re: (no subject)

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Hi Keith,

Send us your name and address and we'll send you a copy of the beautiful
"Microscope Book", a catalog with a lot of information. Or use our 800
#, 440-0311.

Hope all your horses are winners.

Ellie Solit
The Cambrex Group


On Mon, 25 Mar 1996, Keith Lewis Allison, Jr. wrote:

} Hello!
} I have a small horse breeding operation and am just beginning to use
} artificial insemination. I am in need of a microscope for semen
} evaluation. I would appreciate any advice you may be able to provide on
} the purchase, i.e. objective size, source, options, etc. Please keep in
} mind that I am ignorant of microscopy terminology. I do not want
} anything exceptionally fancy, but must be functional....and "tough" for
} farm use. Thanks in advance for your response!
} Keith
}
}




From: Dave King (607)857-1248 T37/257-3 Ext DEKING-at-VNET.IBM.COM
Date: 26 Mar 1996 12:24:26 EST
Subject: Polaron sputter coater

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Message-Id: {199603261722.LAA03719-at-Sparc5.Microscopy.Com}

To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}
*** Reply to note of 03/26/96 11:50

The sputter targets I've seen are thin, and when you see them
starting to come apart, it's time to change them, if you're
concerned about minor contaminants from the base material (I
think ours is Al).

Try Ernest Fullam, SPI, etc for replacement targets.

{
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: Lesley S. Smith :      lesleys-at-pobox.upenn.edu
Date: Tue, 26 Mar 1996 14:27:00 -0500 (EST)
Subject: For Sale!!

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Zeiss TEM-900 for Sale

-Turbomolecular pump, sheet film camera, extras as well.
-asking $35,000. or make an offer.
-call 904-775-4330




From: Douglas F Bowling :      al428-at-dayton.wright.edu
Date: Tue, 26 Mar 1996 14:50:52 -0500
Subject: SEM's in High Schools

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I am a high school teacher of Electronics, who also has a Amray 1700 SEM as a
Science enrichment class for Dayton City Schools in Dayton, OH.

Am interested to know if others out there have an SEM in their high schools.

Hoping to compare notes and ideas.

Thanks,
Doug




From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Wed, 27 Mar 1996 10:03:45
Subject: Re: SEM, ESEM examination of soils

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Hello out there,
Local microbiologists want to look at soil samples and the resident microboal
flora. My EM lab has all the usual SEM and TEM methods on tap and are doing a
library search but if anyone out there has personal experience of

A: looking for (or at) microbes in soil (ON soil) with frozen-hydrated
specimens or

B: untreated soil in an environmental SEM.

we would be very interested to learn of your experiences.
Thanks in advance,

Mel Dickson.




From: dbd1-at-uclink4.berkeley.edu
Date: Tue, 26 Mar 1996 15:43:41 -0800 (PST)
Subject: need section counter

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To all,

I am looking for a section counter accessory for the Reichert Ultra Cut E.
Anybody out there have one that is not being used?


Doug Davis
Staff Research Associate
Electron Microscope Facility
University of California
Berkeley, CA 94720
(510) 642-2085
dbd1-at-uclink4.berkeley.edu







From: Linda Fox :      lfox1-at-wpo.it.luc.edu
Date: Tue, 26 Mar 1996 09:39:47 -0600
Subject: Polaron sputter coater

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Message-Id: {s157bb1f.039-at-wpo.it.luc.edu}
X-Mailer: Novell GroupWise 4.1

Can anyone tell me when it is time to purchase a new Au target for
our Polaron E5100 Series II sputter coater? Ours seems cracked and
peeling in places. Over time it is hard to say if there has been a
difference in coating. There has been a difference in the current we
use to sputter coat. Also, if anyone knows who carries a line of
Polaron parts, that information would be helpful as well.
THANKS
Linda Fox
Loyola University Medical School
lfox1-at-wpo.it.luc.edu





From: Deborah Holmberg :      dlholmberg-at-ucdavis.edu
Date: Tue, 26 Mar 1996 10:59:43 -0800 (PST)
Subject: Re: Scanning 96, student volunteers

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On Thu, 7 Mar 1996, Deborah Holmberg wrote:

} Hello All,
} The Scanning 96 Meeting in Monterey, CA on 9-12 April is looking for
} students to monitor the sessions, ie. run the slide projectors, adjust
} lights and hand out information. The student will have the registration
} fee waived, in appreciation of helping. For information, please contact
} me, or the Scanning 96 office at FAMS, Inc., PO Box 832, Mahwah, NJ
} 07430-0832. E-mail: fams-at-holonet.net
} Thank you,
} Debe Holmberg
} USDA-ARS
} 916.752.9021
} 916.752-4604 (fax)
}




From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Tue, 5 Mar 1996 12:29:18 -0500 (EST)
Subject: Used TEM for sale

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Just wanted to post one last time before we quit.

---------- Forwarded message ----------



Recently, I sent a message to you all about a used TEM, to see if there
was any interest. I have had some, so here is the real offer.

We have a Philips 201c TEM for sale, it is approximately 20 years old.
It is functional, but lack of use has caused the vacuum to be less than
optimal. With a little TLC this scope could be an excellent workhorse.
The investigators who own the scope would be willing to sell it for
parts, but they want it removed from their facility, not just scavenged,
as they need to make room for a new scope.

Thanks in advance

Cheri Owen
Wayne State University
Detroit Neurotrauma Institute
Detroit, MI
(313)577-4648





From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Tue, 26 Mar 1996 19:12:57 -0600
Subject: Re: EMPA: anybody ever done hair?

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} Does anyone out there have any experience with EMPA of hair? Preparation,
} mounting, etc? Someone here is interested in looking at heavy metals that
} thru metabolism are concentrated in the hair.The levels will be low, so
} the MDL will be a question, but I don't know how the hair will stand up to
} an electron beam.
}
} John
}
}
} John Fournelle

I just recently did some dog hair. It seemed to hold up well under
a 10kV beam. This was for imaging, so was Au/Pd coated, however.
Carbon-coating will be more interesting, but should still work. Since your
person wants heavy metals, it might be worthwhile using low kVs and going
for M-lines. Try obilgue or cross-sectioned hairs--shaft exteriors don't
seem to do so well for x-ray; least I've never gotten anything from them.
Phil Oshel

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: Leah Dobbs :      leadob-at-execpc.com
Date: Tue, 26 Mar 1996 21:39:20 -0800
Subject: unsubscribe

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Message-ID: {3158D488.7F8A-at-execpc.com}

unsubscribe




From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Tue, 26 Mar 1996 16:34:52 GMT
Subject: Re: Stereology (apology)

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Sorry guys. I must have been absent the day they taught us
to read



} At 12:28 PM 3/26/96 -0200, you wrote:
} } Are there IBM compatible PC based programmes able to assist with doing
} } stereology from TEM and LM micrographs. What are the hardware require-
} } ments?
} }
} } Thank you
} } Leon Scott
} } SA
} }
} }
} You will find an archived discussion about the presentation
of stereo pairs on the web page listed at the bottom of this message. Look
in the "Tips & Tricks" section. There was some discussion about software. I
will be more than happy to e-mail the file to you as well if you are unable
to access the page.
} Cheers
}




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/





From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Wed, 27 Mar 1996 00:24:51 EST
Subject: Sputter coater targets

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

Linda Fox wrote:

Can anyone tell me when it is time to purchase a new Au target for our
} Polaron E5100 Series II sputter coater? Ours seems cracked and
peeling in
} places. Over time it is hard to say if there has been a difference
in
} coating. There has been a difference in the current we use to
sputter
} coat. Also, if anyone knows who carries a line of Polaron parts,
that
} information would be helpful as well.

It sure does sound like you need a new cathode.

The Polaron line is now being produced by VG Microtech in the UK. The
"official" distributor for VG Microtech in the USA is Energy Beam
Sciences.

Several other firms, such as SPI Supplies are also manufacturing
replacement targets for these units. If your system takes the 57 mm
solid disc, the SPI replacement cathodes differ from the original
equipment cathodes in that the SPI version is 10 mils thick (instead of
3 or 5 mils). Don't forget that SPI offers a precious metals recycling
program, granting a 10% discount when the spent cathode is returned at
the time of purchase of the new cathode.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Wed, 27 Mar 1996 00:23:17 EST
Subject: Embedding w/ LR White

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

The following was posted recently:

No plastic capsule or tube that I know of is air-tight enough for LR
White.You may double emded with gelatin and get away with a plastic
capsule.
Kate Connolly

Standing in our exhibit booth, and listening to how some of our more
ingenuous customers use some of our products, I have learned that if
one takes the UV transparent SPI embedding (silicone) molds, and over
fills them slightly, and then places another (identical) mold on top,
the capillary action between the two molds really does seal out oxygen
to the point that the resin can be UV cured without worry that oxygen
will some how interfere.

Of course, after polymerization, the top mold separates easily and the
blocks are removed from the "bottom" mold.

There are a number of advantages to using this approach since it is far
more easy to properly align samples in a specific direction with
respect to what will eventually become the cutting direction.

More information about the SPI Supplies silicone embedding molds can be
found in our electronic catalog on the WWW (see below).

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: Stephan Coetzee :      stephan-at-gecko.biol.wits.ac.za
Date: Wed, 27 Mar 1996 09:13:03 GMT+2
Subject: Re: Polaron sputter coater

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Linda Fox wrote:

} Can anyone tell me when it is time to purchase a new Au target for
} our Polaron E5100 Series II sputter coater? Ours seems cracked and
} peeling in places. Over time it is hard to say if there has been a
} difference in coating. There has been a difference in the current we
} use to sputter coat. Also, if anyone knows who carries a line of
} Polaron parts, that information would be helpful as well.
} THANKS

Here we using the same model. Normally there is a area which the
target suffer a higher rate of sputtering material loss. That is normally
at a area at the edge along the perimeter of the target. It eventually
leeds to a groove, exposing the target ring. When that happens we
replace ours. We buy the Au or Au/Pd in sheets locally and do it our
selves. It is cheaper and saves the time spent waiting for the
target to arrive. (If it is not in stock we wait 3-8 weeks!) Note
that the sheets can be difficult to cut, especially the Au/Pd

Hope this helps.


Stephan H Coetee
Electron Microscope Unit
Private Bag 3
Wits
2050 Stephan-at-Gecko.biol.WITS.ac.za

Tell: (011) 716 2419
Fax : (011) 339 3407




From: M.F. Butler :      mfb12-at-cus.cam.ac.uk
Date: Wed, 27 Mar 1996 08:50:52 +0000 (GMT)
Subject: unsubscribe

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unsubscribe please




From: Dirk.Voeste-at-rz.ruhr-uni-bochum.de
Date: Wed, 27 Mar 1996 10:41:05 +0000
Subject: TEM-fixation of waterplants

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Hi Everyone,
I am working on a project with TEM investigations of higher waterplants.
Untill now I have only a few experiences with electronmicroscopical
analyis. In my lab we tried to fix the waterplants with large
gaslacunae in the leaves and only two to three cell layers arround
it. We have always the problem that the tissue collapse by
evacuating the leaves or embedding them into Epon, Spurr etc., when
they are not evacuated. The fixation and contrast of cytoplamsic structures
are OK.
Has anyone an idea or knows a method to solve the problem of tissue
collapsing? Is a there a paper published where this phenomen or a appropriate
method has been described for submers waterplants, especially with large
gaslacunae?

Thanks for your help!

Dirk Voeste


Dr. D. Voeste
Ruhr-Univercity Bochum
Comparative Endocrinology Research Section
ND-5/31
D-44780 Bochum
0234/7004325 (phone)
0234/7094551 (fax)
dirk.voeste-at-rz.ruhr-uni-bochum.de




From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 27 Mar 96 08:19:18 EST
Subject: Re: Polaron sputter coater

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Jean-Pierre Slakmon {jps-at-soquelec.com}

Linda-
The Polaron range of specimen preparation equipment for electron microscopy is
manufactured by VG Microtech in the U.K. We (Energy Beam Sciences) are the
exclusive representative for the Polaron range in the United States; Soquelec
Ltd. are the exclusive representatives in Canada. We provide authorized bench
service for current and all older models of Polaron instruments, and carry a
wide range of spare parts in inventory, including targets for all models of
sputter coaters.
The current Polaron range is described at our WWW site
(http://www.ebsciences.com/).
Please contact me directly if I can be of further assistance.
Best regards,
Steven E. Slap, Vice-President
75767,640-at-compuserve.com





From: John Gabrovsek :      gabrovj-at-cesmtp.ccf.org
Date: Wed, 27 Mar 1996 09:25:15 -0500
Subject: TEM

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Message-Id: {s1590bd2.019-at-cesmtp.ccf.org}
X-Mailer: Novell GroupWise 4.1

Hi everybody,
A friend of mine would like to acquire an RCA EMT electron microscope
if they are still available. If they are please get in touch directly
with him. Mr. Tom Bunch e-mail: { tbunch2-at-ix.netcom.com }
Regards,
John Gabrovsek
CCF Cleveland,Ohio





From: Richard Beanland +44 1327 356363 :      richard.beanland-at-gecm.com
Date: Wed, 27 Mar 1996 16:46:22 +0000 (GMT)
Subject: Gold sputter targets

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Disclose-Recipients: prohibited

Seeing Linda Fox's post just reminded me of something I found out the other
day here. Apparently the cheapest source of gold (99.9% pure) is new 'limited
edition' gold coins. Don't ask me why! One guy here had a crown about an
inch across which he then rolled to produce a 2 inch target for an industial
coater. Saved the company a packet.


Richard Beanland
GMMTL Caswell,
Towcester,
Northants NN12 8EQ.

Tel. +44 1327 356363
Fax. +44 1327 356775
Email richard.beanland.gecm.com





From: Igor Polyakov :      Igor_Polyakov-at-qmgate.arc.nasa.gov
Date: 27 Mar 1996 09:15:22 -0800
Subject: freezing

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Message-Id: {n1384204335.95012-at-qmgate.arc.nasa.gov}

Subject: Time:9:42 AM
OFFICE MEMO freezing Date:3/26/96

I am looking for available information about freezing a tissue(brain) for LM
histochemistry and immunocytochemistry. Also, I need information how to
estimate the optical density of a histochemically stained sections? Are there
books, those could give me such information?
Any information would be very much appreciated.
Igor_Polyakov-at-qmgate.arc.nasa.gov






From: John Phelps :      phelps-at-enh.nist.gov
Date: Wed, 27 Mar 1996 11:41:09 -0700 (MST)
Subject: Sample prep - Glue Sources?

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Good day all,

We have been using Gatan "G-1" epoxy to prepare cross sections for TEM
analysis. Does anyone know what this "G-1" epoxy is? More importantly, is
this an epoxy that be purchased at, say a hardware store? Or perhaps some
one knows of a substitute that works as well or possibly better that can be
purchased locally.

thanks in advance,
John

John Phelps
NIST
Materials Reliability Division
325 Broadway
Boulder, CO 80303
ph. 303-497-7570
fax. 303-497-5030




From: Hand-at-nso1.uchc.edu (Hand,Arthur)
Date: Wed, 27 Mar 1996 12:01:07 -0500
Subject: EM Facility User Fees

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Thanks to all who responded to my request for information on 3/11/96. I have
compiled a list of fees for selected services for 12 USA facilities and 4
facilities in other countries. If you would like a copy, let me know if you
prefer to receive it by FAX or by e-mail (Mac or IBM format).

Arthur R. Hand
Central EM Facility
Univ of Connecticut Health Ctr
e-mail: hand-at-nso1.uchc.edu





From: MICHAEL KNOTTS :      ph281mk-at-prism.gatech.edu
Date: Wed, 27 Mar 1996 15:39:07 -0500 (EST)
Subject: Gold sources

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A note related to Richard Beanland's comment about using a flattened gold
coin as a sputter target to save money:

I use 1/4 oz Canadian Maple coins (purchased at a local coin dealer) as a
source of gold (99.99% pure) in my thermal vacuum evaporation system. I
cut them into small pie-shaped pieces using heavy duty wire cutters that
have been carefully cleaned and degreased. These pieces can be futher
subdivided prior to weighing them and placing them in the evaporator's
tungsten boat.

---------------------------------------------------------
Dr. Michael E. Knotts E-mail: ph281mk-at-prism.gatech.edu
Georgia Tech / School of Physics / Atlanta, GA 30332-0430
Tel: (404) 894-3422 FAX: (404) 894-9958




From: Damon Heer :      DLH-at-fei2.feico.com
Date: Wed, 27 Mar 1996 13:21:22 -0800
Subject: Focused Ion Beam Analysis

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Greetings list server friends. A recent request regarding FIB
information caught my eye and I forwarded it to a colleague who
works in our FIB systems division. His input is below. In an
effort to not clog up the list server with marketing or sales junk,
the response is mostly an offering of where to go to find further
information.

Cheers,
Damon

**************************************

Hello,

This indeed sounds like a FIB-related application. I am a Sales
Associate at FEI. As you may know, FEI specializes in the
development and production of FIB systems and their components. We
would be happy to answer any questions you have regarding this
technology.

In response to your friend's situation, we have an on-site lab that can
analyze any number of different types of samples, so please contact
me if you think I can be of assistance.

If you, your colleagues, or any others on the list server have any
additional FIB related inquiries, please feel free to e-mail me
directly, off line from this list server, at any time.

Brinker B. Gildersleeve
Sales Associate
FEI Company

bbg-at-feico.com



} Greetings,
}
} A colleague is needing compositional analysis from 10 nm layers. He is
} interested in doing focused ion beam analysis considering sample prep
} difficulties with TEM. Since I am not familiar with FIB, is this a viable
} technique for his problem? If it is, is there anyone out there willing to
} analyze his samples?
}
} Jeff Shield


FEI Company
7451 N.E. Evergreen Parkway
Hillsboro, OR 97124-5830

Phone (503) 640-7582
fax (503) 640-7509
email dlh-at-feico.com




From: Scott Holt :      102467.2752-at-CompuServe.COM
Date: 27 Mar 96 17:22:12 EST
Subject: EM Glue Suppliers

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John Phelps wrote:

} We have been using Gatan "G-1" epoxy to prepare cross sections for TEM
} analysis. Does anyone know what this "G-1" epoxy is? More importantly, is
} this an epoxy that be purchased at, say a hardware store? Or perhaps some
} one knows of a substitute that works as well or possibly better that can be
} purchased locally.

} thanks in advance,
} John

} John Phelps
} NIST
} Materials Reliability Division
} 325 Broadway
} Boulder, CO 80303
} ph. 303-497-7570
} fax. 303-497-5030

John,

G-1 Epoxy appears to be the same as EPOXY TECHNOLOGY's 353ND epoxy. This
adhesive
was originally sold for fiber optic work. Anyway, we at BUEHLER sold it until
recently
when it's carcinogenic properties became better known to us. However, I believe
you
can still buy it directly from EPOXY TECHNOLOGY INC. in Billerica, MA (Phone:
1-800-227-2201).
Last time I bought some, I got a good sized can (without quantity written on the
label) estimated
at about 15-20oz. for $35.00. However, there are other EM suppliers who sell it
in smaller
containers for a somewhat reasonable price.

Good Luck.
Scott D. Holt
BUEHLER
41 Waukegan Rd.
Lake Bluff, IL 60044
(847)295-4546





From: slakmon-at-scottscientific.com (P. Slakmon)
Date: Wed, 27 Mar 1996 09:40:47 -0500
Subject: Conference & Workshop Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


S C O T T S C I E N T I F I C


On the behalf of The International Society of Molecular Morphology

Would like to take the opportunity to announce the

FOURTH INTERNATIONAL CONFERENCE & WORKSHOP
on
MOLECULAR MORPHOLOGY

June 3-4, 1996 - Conference
June 5 -6, 1996 - Workshop
in

Montreal, Canada

CONFERENCE FEATURING
Advances in Principles, Techniques and
Applications in Research and Diagnosis of:

- In Situ PCR
- In Situ Hybridization
- Immunohistochemistry
- Immunogold-Silver Staining
- Immunogold Electron Microscopy
- Microwave Immunohistochemistry
- Atomic Force Probe Microscopy
- Confocal Microscopy
- Antigen Retrieval
- Image Analysis

Call for Abstract Submission
DEADLINE: APRIL 15, 1996

The abstract should be typed single space on white paper within 9 x 7 inch
(23 x 18 cm) typed space. Total of two pages per abstract. Photographs and
references may be included. Please follow the style of CELL VISION, in
which the Proceedings will be published. Abstracts should be submitted in
duplicate.

THREE HANDS-ON WORKSHOPS (at the laboratories of Dept. of Anatomy,
University of Montreal)
1 In Situ PCR and In Situ Hybridization
2 Immunogold EM and Immunogold-Silver Staining
3 Microwave Fixation, Antigen Retrieval and Immunohistochemistry

June 2, 1996 (Sunday), 6.30-9.30 pm:
An optional preparation lecture on "Molecular Biology for the Uninitiated"

ORGANIZING COMMITTEE
CO-CHAIRMEN
Jiang Gu, M.D., Ph.D.
Deborah Research Institute
Browns Mills, New Jersey, USA

Moise Bendayan, Ph.D.
University of Montreal
Montreal, Canada

MEMBERS
Virginia Anderson, M.D.
Health Science Center at Brooklyn
State University of New York
Brooklyn, New York, USA

Gerhard Hacker, Ph.D.
Institute of Pathology
General Hospital, University of Salzburg
Salzburg, Austria

Lawrence DeBault, Ph.D.
Oklahoma University Health Center
Oklahoma City, Ok, USA

Shahla Masood, M.D.
University of Florida Health Science Center
Jackonsville, Florida, USA

Robert Day, Ph.D.
University of Montreal
Montreal, Canada

David Kersten, M.D.
Ealing
London, UK

----------------------------------------------------------------------------
-------------------------------------
ADVANCED REGISTRATION FORM
(please print and use photocopies for additional forms)

NAME ________________________________

PHONE _________________ FAX __________________

ADDRESS __________________________________________

_________________________________________

CHOICE OF WORKSHOP (circle one): 1 2 3
----------------------------------------------------------------------------
-------------------------------------
ADVANCED REGISTRATION FREE (for one of three):
- For the two-day conference $250 (US)
- For the two-day workshop $350 (US)

Make check payable to CELL VISION. A 15 % discount for members of the
"International Society of Molecular Morphology" and students (with proven
ID). A 15% discount will be reimbursed upon becoming a member of the
society before or at the conference.
Please book the hotel room directly by calling The Best Western Hotel in
Montreal, Canada (800) 361-3000 or Fax (514) 861-4089. You can obtain a
special discounted room rate at $79 (Canadian) per day (rate includes
breakfast) by identifying yourself as a participant of the conference/workshop.
Student dormitory available at University of Montreal (15-minute subway
transporation to conference location, 5 minute walk to workshop location) at
$35 (Canadian) per day by calling (514) 343-6531.

Send abstract, registration form, and registration fee to:
CELL VISION-JOURNAL OF ANALYTICAL MORPHOLOGY,
EDITORIAL OFFICE, DEBORAH RESEARCH INSTITUTE
1 Trenton Road, Browns Mills
NJ 08015-1799, USA
Phone: (609) 735-0477
Fax: (609) 735-0478


For further information please direct your inquiries by email to:
morphology-at-scottscientific.com
_______________________________________________
SCOTT SCIENTIFIC
P.O. Box 66552 Station Cavendish,
Montreal, Quebec, H4W 3J6, Canada

Telephone: 514-485-2309 Fax: 514-485-9931
Voice Mail: 514-888-6509

WWW Site: http://www.ScottScientific.com

E-Mail: slakmon-at-scottscientific.com
info-at-scottscientific.com
sales-at-scottscientific.com
admin-at-scottscientific.com
_________________________________________________





From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Wed, 27 Mar 1996 17:45:59 -0600
Subject: Atlas of prokaryotes

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


I need a reference to a through TEM/SEM atlas of ultrastructure of
prokaryotes and viruses. Or separate atlases for each.
Must be 1990 or later.
Thanks in advance for any info.
Phil

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 3/26/96 8:54 AM
Subject: EMPA: anybody ever done hair?

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html



Does anyone out there have any experience with EMPA of hair? Preparation,
mounting, etc? Someone here is interested in looking at heavy metals that
thru metabolism are concentrated in the hair.The levels will be low, so
the MDL will be a question, but I don't know how the hair will stand up to
an electron beam.

John


John Fournelle
Electron Microprobe Lab Internet:johnf-at-geology.wisc.edu
Dept of Geology & Geophysics Office: (608) 262-7964
University of Wisconsin Lab: (608) 265-4798
1215 West Dayton Street Fax: (608) 262-0693
Madison, WI 53706 Amateur radio: WA3BTA/9
http://geology.wisc.edu/~johnf/sx51.html

"The first rule of all intelligent tinkering is to save all the pieces."
Aldo Leopold








From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 27 Mar 96 17:58:28 EST
Subject: Epoxy for TEM Sample Prep

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


John-

The G1 is the same material we sell as "Golly G1" (we like to have some fun
here!).

The actual material is EpoTek 353ND. It is available from:

Epoxy Technology, Inc.
14 Fortune Drive
Billerica, MA 01821

TEL: 800-227-2201
FAX: 508-663-9782

We sell an 8 ounce kit of our Golly G1 for about $50. However, you can buy it
directly from Epoxy Technology for between $25-30. An 8 ounce kit will last you
a lifetime! I think Gatan sells it iin much smaller containers if that is
appealing (However, I don't think it costs any less).

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
sbt-at-msa.microscopy.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: Beverly E Maleeff
Date: 27 Mar 96 11:17:13 EDT
Subject: April PSM Meeting Notice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {9603271909.AA7252-at-pho903.sbphrd.com}
To: microscopy {microscopy-at-Sparc5.Microscopy.Com}
{Beverly_E_Maleeff-at-sbphrd.com}

Philadelphia Society for Microscopy Meeting Notice
April 1996


DATE: Wednesday, April 10, 1996

PLACE: Laboratory for the Research of Science and Materials
(LRSM) Building, 33rd and Walnut Street, Philadelphia, PA.
Parking is available behind the LRSM Building after 5:00 PM.


TIME: 5:30 - 7:30 PM Social hour, hosted by our meeting sponsor.
Buffet dinner service and informal seating during this time

7:30 PM Speaker:

Photooxidation of Fluorescent Markers:
A Bridge Between Light and Electron Microscopic Cytochemistry

Dr. Giuseppe G. Pietra
Professor of Pathology
Hospital of the University of Pennsylvania
Philadelphia, PA

Abstract:
Fluorescent labeling techniques have become very popular in many areas of
pathology and cell biology. However, a limitation of fluorescent labeling is
the relative low resolution of the optical microscope, even using laser
scanning confocal microscopy. Excitation of a fluorescent dye in the presence
of 3-3-diaminobenzidine (DAB) oxidizes DAB to an insoluble electron-dense
product that can be readily localized by electron microscopy. The application
of this method to biological problems will be illustrated in a correlative
confocal and electron microscopic study of experimental pulmonary edema.
Advantages and limitations of this technique will be discussed.




DINNER: With a Mexican Flair!!

COST: Members $12.00 Student members $6.00 Non-members $15.00

MENU:
Margaritas
Mexican beer
Salsa, chips, pretzels, etc.

Vegetarian chili
Taco shells, crispy or soft (matzoh will be available)
Sauteed ground beef or chicken
Shredded lettuce
Grated cheese
Diced tomatoes
Sour cream
Guacamole
Refried beans
Tortilla chips

Fresh fruit salad

Coffee, decaf or tea


Reservations will be taken by Ms. Pat Overend at the University of
Pennsylvania, 215/898-8337. Deadline for reservations will be Friday, April
5. If you have any questions regarding the meeting please feel free to contact
Rollin Lakis at 215/898-2013 or lakis-at-sol1.lrsm.upenn.edu.
Cancellations must be received by Ms. Overend no later than 5:00 PM, April 5,
1996.






From: David_Gantt-at-GSVMS2.CC.GASOU.EDU (DAVID G. GANTT)
Date: Wed, 27 Mar 1996 20:07:39 -0400
Subject: Tooth buds

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {v01530501ad7f888249c1-at-[141.165.35.119]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I am interested in determining the best procedure to study tooth buds with
the SEM. Any Suggestion!

Dr. David G. Gantt Phone: 1-912-681-5964
Dept. of Biology Fax: 1-912-681-0845
Landrum Box 8042 e-mail: david_gantt-at-gsvms2.cc.gasou.edu
Georgia Southern University
Statesboro, Georgia 30460-8042








From: Sarka Lhotak :      lhotaks-at-fhs.csu.mcmaster.ca
Date: Wed, 27 Mar 1996 19:55:25 -0500 (EST)
Subject: Re: Embedding w/ LR White

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Just a quick note on LR White embedding: We occasionally use flat
embedding with LR White into polyethylene (I believe) molds and cover
the whole thing with Saran wrap. It is a bit messy but it works. I believe
you could overfill the tube sligthly and cover with Saran wrap too.
Cheers,

Sarka Lhotak
EM Facility, McMaster University
Hamilton, Ontario, Canada
lhotaks-at-fhs.mcmaster.ca




From: James S MArtin :      James.S.Martin-at-williams.edu
Date: Wed, 27 Mar 1996 22:24:33 -0500 (EST)
Subject: re: EMPA of hair

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential
Microscopy ListServer {Microscopy-at-Sparc5.Microscopy.Com}
Message-Id: {960327154237.718-at-cliff.ml.wpafb.af.mil.0}

Several years ago, 1991 I think, a forensic scientist from Scotland Yard was
the dinner speaker at Inter-Micro in Chicago. One of the cases he
described involved the poisoning of a woman from the Middle East. He
detected arsenic along a strand of the woman's hair using SEM-EDS. By
correlating the intervals at which arsenic was detected with the growth
rate of the woman's hair and her schedule over the preceding months, he
demonstrated that she was intentially and repeatedly poisoned in the same
city. I recall that this led to an arrest. If you need more info, let
me know.

James Martin
Williamstown Art Conservation Center
Williamstown, MA




From: John M. Libert :      jlibert-at-cpcug.org
Date: Wed, 27 Mar 1996 20:55:25 -0500
Subject: Re: Stereology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-ID: {3159F18D.342A-at-cpcug.org}

scott_l-at-unin1.unorth.ac.za wrote:
}
} Are there IBM compatible PC based programmes able to assist with doing
} stereology from TEM and LM micrographs. What are the hardware require-
} ments?
}
} Thank you
} Leon Scott
} SAYes. Assuming your images are in TIFF or other common format, the
Bioquant software from R&M Biometrics has an excellent stereology toolkit
add-on module to the True Color Windows image analysis and topographic
morphometry software package. R&M likes to provide the PC integrated with
the software and the high-end frame grabber that they use, but the system
can be installed on most 486 or Pentium computers able to run Windows.
Generally, 16MB RAM and large disk capacity (1 GByte) is recommended for
image work. If you wish, I can help you locate the BioQuant dealer in
your area. Or, better you can call R&M's office in Nashville, TN at
1-800-221-0549. They will be happy to send you literatue and even to
arrange for a demonstration for you.

Regards,

John Libert




From: John M. Libert :      jlibert-at-cpcug.org
Date: Wed, 27 Mar 1996 20:55:25 -0500
Subject: Re: Stereology

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-ID: {3159F18D.342A-at-cpcug.org}

scott_l-at-unin1.unorth.ac.za wrote:
}
} Are there IBM compatible PC based programmes able to assist with doing
} stereology from TEM and LM micrographs. What are the hardware require-
} ments?
}
} Thank you
} Leon Scott
} SAYes. Assuming your images are in TIFF or other common format, the
Bioquant software from R&M Biometrics has an excellent stereology toolkit
add-on module to the True Color Windows image analysis and topographic
morphometry software package. R&M likes to provide the PC integrated with
the software and the high-end frame grabber that they use, but the system
can be installed on most 486 or Pentium computers able to run Windows.
Generally, 16MB RAM and large disk capacity (1 GByte) is recommended for
image work. If you wish, I can help you locate the BioQuant dealer in
your area. Or, better you can call R&M's office in Nashville, TN at
1-800-221-0549. They will be happy to send you literatue and even to
arrange for a demonstration for you.

Regards,

John Libert




From: slakmon-at-scottscientific.com (P. Slakmon)
Date: Wed, 27 Mar 1996 19:44:00 -0500
Subject: Conference & Workshop Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


S C O T T S C I E N T I F I C


On the behalf of The International Society of Molecular Morphology

Would like to take the opportunity to announce the

FOURTH INTERNATIONAL CONFERENCE & WORKSHOP
on
MOLECULAR MORPHOLOGY

June 3-4, 1996 - Conference
June 5 -6, 1996 - Workshop
in

Montreal, Canada

CONFERENCE FEATURING
Advances in Principles, Techniques and
Applications in Research and Diagnosis of:

- In Situ PCR
- In Situ Hybridization
- Immunohistochemistry
- Immunogold-Silver Staining
- Immunogold Electron Microscopy
- Microwave Immunohistochemistry
- Atomic Force Probe Microscopy
- Confocal Microscopy
- Antigen Retrieval
- Image Analysis

Call for Abstract Submission
DEADLINE: APRIL 15, 1996

The abstract should be typed single space on white paper within 9 x 7 inch
(23 x 18 cm) typed space. Total of two pages per abstract. Photographs and
references may be included. Please follow the style of CELL VISION, in
which the Proceedings will be published. Abstracts should be submitted in
duplicate.

THREE HANDS-ON WORKSHOPS (at the laboratories of Dept. of Anatomy,
University of Montreal)
1 In Situ PCR and In Situ Hybridization
2 Immunogold EM and Immunogold-Silver Staining
3 Microwave Fixation, Antigen Retrieval and Immunohistochemistry

June 2, 1996 (Sunday), 6.30-9.30 pm:
An optional preparation lecture on "Molecular Biology for the Uninitiated"

ORGANIZING COMMITTEE
CO-CHAIRMEN
Jiang Gu, M.D., Ph.D.
Deborah Research Institute
Browns Mills, New Jersey, USA

Moise Bendayan, Ph.D.
University of Montreal
Montreal, Canada

MEMBERS
Virginia Anderson, M.D.
Health Science Center at Brooklyn
State University of New York
Brooklyn, New York, USA

Gerhard Hacker, Ph.D.
Institute of Pathology
General Hospital, University of Salzburg
Salzburg, Austria

Lawrence DeBault, Ph.D.
Oklahoma University Health Center
Oklahoma City, Ok, USA

Shahla Masood, M.D.
University of Florida Health Science Center
Jackonsville, Florida, USA

Robert Day, Ph.D.
University of Montreal
Montreal, Canada

David Kersten, M.D.
Ealing
London, UK

----------------------------------------------------------------------------
-------------------------------------
ADVANCED REGISTRATION FORM
(please print and use photocopies for additional forms)

NAME ________________________________

PHONE _________________ FAX __________________

ADDRESS __________________________________________

_________________________________________

CHOICE OF WORKSHOP (circle one): 1 2 3
----------------------------------------------------------------------------
-------------------------------------
ADVANCED REGISTRATION FREE (for one of three):
- For the two-day conference $250 (US)
- For the two-day workshop $350 (US)

Make check payable to CELL VISION. A 15 % discount for members of the
"International Society of Molecular Morphology" and students (with proven
ID). A 15% discount will be reimbursed upon becoming a member of the
society before or at the conference.
Please book the hotel room directly by calling The Best Western Hotel in
Montreal, Canada (800) 361-3000 or Fax (514) 861-4089. You can obtain a
special discounted room rate at $79 (Canadian) per day (rate includes
breakfast) by identifying yourself as a participant of the conference/workshop.
Student dormitory available at University of Montreal (15-minute subway
transporation to conference location, 5 minute walk to workshop location) at
$35 (Canadian) per day by calling (514) 343-6531.

Send abstract, registration form, and registration fee to:
CELL VISION-JOURNAL OF ANALYTICAL MORPHOLOGY,
EDITORIAL OFFICE, DEBORAH RESEARCH INSTITUTE
1 Trenton Road, Browns Mills
NJ 08015-1799, USA
Phone: (609) 735-0477
Fax: (609) 735-0478


For further information please direct your inquiries by email to:
morphology-at-scottscientific.com
_______________________________________________
SCOTT SCIENTIFIC
P.O. Box 66552 Station Cavendish,
Montreal, Quebec, H4W 3J6, Canada

Telephone: 514-485-2309 Fax: 514-485-9931
Voice Mail: 514-888-6509

WWW Site: http://www.ScottScientific.com

E-Mail: slakmon-at-scottscientific.com
info-at-scottscientific.com
sales-at-scottscientific.com
admin-at-scottscientific.com
_________________________________________________





From: slakmon-at-scottscientific.com (P. Slakmon)
Date: Wed, 27 Mar 1996 19:34:37 -0500
Subject: Conference & Workshop Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


S C O T T S C I E N T I F I C


On the behalf of The International Society of Molecular Morphology

Would like to take the opportunity to announce the

FOURTH INTERNATIONAL CONFERENCE & WORKSHOP
on
MOLECULAR MORPHOLOGY

June 3-4, 1996 - Conference
June 5 -6, 1996 - Workshop
in

Montreal, Canada

CONFERENCE FEATURING
Advances in Principles, Techniques and
Applications in Research and Diagnosis of:

- In Situ PCR
- In Situ Hybridization
- Immunohistochemistry
- Immunogold-Silver Staining
- Immunogold Electron Microscopy
- Microwave Immunohistochemistry
- Atomic Force Probe Microscopy
- Confocal Microscopy
- Antigen Retrieval
- Image Analysis

Call for Abstract Submission
DEADLINE: APRIL 15, 1996

The abstract should be typed single space on white paper within 9 x 7 inch
(23 x 18 cm) typed space. Total of two pages per abstract. Photographs and
references may be included. Please follow the style of CELL VISION, in
which the Proceedings will be published. Abstracts should be submitted in
duplicate.

THREE HANDS-ON WORKSHOPS (at the laboratories of Dept. of Anatomy,
University of Montreal)
1 In Situ PCR and In Situ Hybridization
2 Immunogold EM and Immunogold-Silver Staining
3 Microwave Fixation, Antigen Retrieval and Immunohistochemistry

June 2, 1996 (Sunday), 6.30-9.30 pm:
An optional preparation lecture on "Molecular Biology for the Uninitiated"

ORGANIZING COMMITTEE
CO-CHAIRMEN
Jiang Gu, M.D., Ph.D.
Deborah Research Institute
Browns Mills, New Jersey, USA

Moise Bendayan, Ph.D.
University of Montreal
Montreal, Canada

MEMBERS
Virginia Anderson, M.D.
Health Science Center at Brooklyn
State University of New York
Brooklyn, New York, USA

Gerhard Hacker, Ph.D.
Institute of Pathology
General Hospital, University of Salzburg
Salzburg, Austria

Lawrence DeBault, Ph.D.
Oklahoma University Health Center
Oklahoma City, Ok, USA

Shahla Masood, M.D.
University of Florida Health Science Center
Jackonsville, Florida, USA

Robert Day, Ph.D.
University of Montreal
Montreal, Canada

David Kersten, M.D.
Ealing
London, UK

----------------------------------------------------------------------------
-------------------------------------
ADVANCED REGISTRATION FORM
(please print and use photocopies for additional forms)

NAME ________________________________

PHONE _________________ FAX __________________

ADDRESS __________________________________________

_________________________________________

CHOICE OF WORKSHOP (circle one): 1 2 3
----------------------------------------------------------------------------
-------------------------------------
ADVANCED REGISTRATION FREE (for one of three):
- For the two-day conference $250 (US)
- For the two-day workshop $350 (US)

Make check payable to CELL VISION. A 15 % discount for members of the
"International Society of Molecular Morphology" and students (with proven
ID). A 15% discount will be reimbursed upon becoming a member of the
society before or at the conference.
Please book the hotel room directly by calling The Best Western Hotel in
Montreal, Canada (800) 361-3000 or Fax (514) 861-4089. You can obtain a
special discounted room rate at $79 (Canadian) per day (rate includes
breakfast) by identifying yourself as a participant of the conference/workshop.
Student dormitory available at University of Montreal (15-minute subway
transporation to conference location, 5 minute walk to workshop location) at
$35 (Canadian) per day by calling (514) 343-6531.

Send abstract, registration form, and registration fee to:
CELL VISION-JOURNAL OF ANALYTICAL MORPHOLOGY,
EDITORIAL OFFICE, DEBORAH RESEARCH INSTITUTE
1 Trenton Road, Browns Mills
NJ 08015-1799, USA
Phone: (609) 735-0477
Fax: (609) 735-0478


For further information please direct your inquiries by email to:
morphology-at-scottscientific.com
_______________________________________________
SCOTT SCIENTIFIC
P.O. Box 66552 Station Cavendish,
Montreal, Quebec, H4W 3J6, Canada

Telephone: 514-485-2309 Fax: 514-485-9931
Voice Mail: 514-888-6509

WWW Site: http://www.ScottScientific.com

E-Mail: slakmon-at-scottscientific.com
info-at-scottscientific.com
sales-at-scottscientific.com
admin-at-scottscientific.com
_________________________________________________





From: Bo Johansen :      BOJ-at-bot.ku.dk
Date: Thu, 28 Mar 1996 11:06:08 GMT+0100
Subject: Elmers glue

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Hello - is there anybody out there who can tell me where
to obtain 'Elmers glue'.

Bo


_____________________________________________________________________
Bo Johansen E-Mail: BoJ-at-bot.ku.dk
Botanical Institute Vioce: +45 3532 2157
Gothersgade 140 FAX: +45 3313 9104
DK-1123 Copenhagen K, Denmark http://www.bot.ku.dk/www/staff/boj.htm
---------------------------------------------------------------------






From: James S MArtin :      James.S.Martin-at-williams.edu
Date: Thu, 28 Mar 1996 08:27:36 -0500 (EST)
Subject: Re: Elmers glue

Contents Retrieved from Microscopy Listserver Archives
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Elmer's Glue is manufactured by Borden, Inc., Columbus 43215 (USA).
Borden makes at least two different types of Elmer's (registered
tradename) glue -- a white glue composed primarily of an aqueous
dispersion of poly(vinyl acetate), and a yellow carpenter's wood glue
composed primarily of an aliphatic resin.

James Martin
Williamstown Art Conservation Center


On Thu, 28 Mar 1996, Bo Johansen wrote:

} Hello - is there anybody out there who can tell me where
} to obtain 'Elmers glue'.
}
} Bo
}
}
} _____________________________________________________________________
} Bo Johansen E-Mail: BoJ-at-bot.ku.dk
} Botanical Institute Vioce: +45 3532 2157
} Gothersgade 140 FAX: +45 3313 9104
} DK-1123 Copenhagen K, Denmark http://www.bot.ku.dk/www/staff/boj.htm
} ---------------------------------------------------------------------
}
}
}




From: colijn.1-at-osu.edu (Henk Colijn)
Date: Thu, 28 Mar 1996 09:01:42 -0500 (EST)
Subject: Post Doc position available

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Post-Poctoral Research Associate
Center for Industrial Sensors and Measurements
The Ohio State University

A post-doctoral research position is presently available in the Department of
Materials Science and Engineering in the area of TEM characterization of
ceramic-based materials. Specifically this position is part of a new NSF
Center for Industrial Sensors and Measurements (CISM). Key components of this
work will be analytical (EELS and EDS) and high resolution TEM investigation of
interfaces in ceramic systems such as those based on TiO2 and Zr2O3. The state-
of-the-art microscopy facilities available in the Department of Materials
Science and Engineering will be utilized for this program. The position will
also involve close interaction with a number of industrial partners who are
collaborating in this effort to develop improved sensors for harsh industrial
environments.

Applicants may send a cirriculum vitae by mail or E-mail to:

Professor Michael J. Mills
Department of Materials Science and Engineering
The Ohio State University
477 Watts Hall
2041 College Road
Columbus, OH 43210-1178

Tel: (614) 292 - 7514
Fax: (614) 292 - 1537

E-mail: mills.108-at-osu.edu

Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility
-------------------------------------------------------------------
An optimist believes that we live in the best of all possible worlds.
A pessimist fears this is true.






From: RMacKay :      RMACKAY-at-AC.DAL.CA
Date: Thu, 28 Mar 1996 10:35:05 +0000
Subject: Re: EMPA: anybody ever done hair?

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Message-Id: {199603281440.KAA09558-at-Snoopy.UCIS.Dal.Ca}
Comments: Authenticated sender is {RMACKAY-at-ac.dal.ca}

John,

R. A. Smith did some work in this area a few years ago. See
" A Method to Distinguish between Arsenic in and on human
Hair ", Environmental Research 12, 171-173 (1976).

Bob MacKay
Robert MacKay
Department of Earth Sciences
Dalhousie University
Halifax, Nova Scotia, Canada
B3H 3J5
Tel: 902 494-7087
e-mail rmackay-at-ac.dal.ca




From: Len Adleman :      adleman-at-pollux.usc.edu
Date: Thu, 28 Mar 1996 10:34:04 -0800 (PST)
Subject: Please unsubscibe

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Message-Id: {9603281448.AA04445-at-crdems.ge.com}

I would like to unsubscribe:
adleman-at-cs.usc.edu
The volume was too great for my modest needs - thnaks




From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 28 Mar 96 12:21:21 EST
Subject: TEM: Copy of Ultramicroscopy paper

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Dear Friends-

Can anyone out there supply me with a copy of the following paper?

"Applications of an Ionless Tripod Polisher to Preparation of YBCO
Superconducting Multilayer and Bulk Ceramic Thin Films" J. Ayache & P. H.
Alboreole.

Ultramicroscopy, 60(2), September 1995

Thank you!

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
sbt-at-msa.microscopy.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 28 Mar 1996 09:20:51 -0400
Subject: RE- CalibStds

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Message-ID: {n1384117519.71286-at-mse.engin.umich.edu}

Subject: Time: 9:14 AM
OFFICE MEMO RE: CalibStds Date: 3/28/96

Bob:
I am afraid that you will find that logical arguments, based on physical
reality, such as you offer regarding the stability of standards for
calibrating optical microscopes, don't wash with the bureaucracies involved
in promulgating and enforcing standardization and safety regulations. Once a
regulation is written to take care of one type of problem, it is applied
rigorously in all other situations, no matter how ridiculous. I recently
heard of a incident in which a safety inspector required a label listing
manufacturer, composition, safety hazards, etc. on a wash bottle that was
clearly marked as containing distilled water.
Wil Bigelow (bigelow-at-umich.edu)





From: Hand-at-nso1.uchc.edu (Hand,Arthur)
Date: Thu, 28 Mar 1996 09:52:35 -0500
Subject: EM User Fees

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--=====================_19263778==_
Content-Type: text/plain; charset="US-ASCII"

Since there have been a large number of requests for the data on user fees, I
have decided to post the information on the listserver as attachments to
this message. They were created in MS Word 6; one attachment is in Mac
format; the other is in PC format. If you have problems opening them, let me
know and I will fax the data to you as soon as possible.

Arthur R. Hand
Central EM Facility
UConn Health
Ctr

--=====================_19263778==_
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M+B!(86YD%4%22"`Y-CI&964-at-0V]M {&%R:7-O;-at-Y! {G1H=7(-at-4BX-at-2&%N9$)-
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M`````0```````!```````-at-````'____^``````````#_________________
M____________________________________________________________
M____________________________________________________________
M____________________________________________________________
6________________________________
`
end


--=====================_19263778==_
Content-Type: text/plain; charset="US-ASCII"


--=====================_19263778==_--




From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 3/28/96 8:37 AM
Subject: Elmers glue

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Hello - is there anybody out there who can tell me where
to obtain 'Elmers glue'.

Bo


_____________________________________________________________________
Bo Johansen E-Mail: BoJ-at-bot.ku.dk
Botanical Institute Vioce: +45 3532 2157
Gothersgade 140 FAX: +45 3313 9104
DK-1123 Copenhagen K, Denmark http://www.bot.ku.dk/www/staff/boj.htm
---------------------------------------------------------------------







From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Thu, 28 Mar 1996 10:32:05 -0500 (EST)
Subject: Re: freezing

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In response to your question for freezing brain tissue. We freeze
brain for cryostat sectioning in our lab. After it is perfused,
(generally in 4% paraformaldehyde) we run the brain through a sucrose
series of 10%, 20% and 30% solution. This is for cryoprotection. We
then make a cup with aluminum foil, place a small amount of Tissue Tek
(freezing medium) in the bottom and place our 2mm slab of whole brain in
the cup with the face we want to section, down. We then submerge the
rest of the slab in Tissue tek and place the cup on dry ice with acetone
poured over the dry ice to make it colder. It freezes pretty rapidly,
and will last in the -20' freezer for a couple of weeks before sectioning.

Let me know if you want more details...

Cheri Owen
Detroit Neurotrauma Institute
Wayne State University
Detroit, MI
313-577-4648

On 27 Mar 1996, Igor Polyakov wrote:

} Subject: Time:9:42 AM
} OFFICE MEMO freezing Date:3/26/96
}
} I am looking for available information about freezing a tissue(brain) for LM
} histochemistry and immunocytochemistry. Also, I need information how to
} estimate the optical density of a histochemically stained sections? Are there
} books, those could give me such information?
} Any information would be very much appreciated.
} Igor_Polyakov-at-qmgate.arc.nasa.gov
}
}
}




From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: Thu, 28 Mar 1996 15:45:52 +0000
Subject: Polaron targets

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To: lfox-at-wpo.it.luc.edu

I am pleased that your Polaron unit continues to give service. The
target life of a system is entirely dependant on the use and there is
not a standard figure anyone can give.

When deterioration of the target is significant as you describe, it
is time to replace it. No real damage will be done to the system,
but the quality will deteriorate and any contamination from behind the
target will be exposed to the plasma.

If you have a medium to high res. SEM or where grain islands from the
gold becomes a problem, then we recommend gold/palladium (SC502-314B)
or platinum (SC502-314C)

Your local agent EBS will be able to offer delivery and price.

If you experience any problems, please contact me.

Best regards
Tony King

Regards,

Tony King
Product specialist
VG Microtech/ Polaron range

Tel: +44 (0)1825 746251
Fax: +44 (0)1825 768343

Disclaimer:
The views and opinions expressed are not necessarily
those of Fisons plc or VG Microtech.







From: Joe D Geller :      geller-at-world.std.com
Date: Thu, 28 Mar 1996 11:38:41 -0500 (EST)
Subject: Calibration Standards

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1) For typical calibration intervals for recertification you must check with
NIST and get their recommendation in writing for your auditors satisfaction. We specify one year intervals for our NIST traceable magnification
reference standards.

2) For NIST recertification services I remember one experience last year where NIST suggested that the purchase price of a new standard (SRM-484) would be comparable to recertifying an existing one. That, combined
with an out of stock situation left the customer with no solution.

3) For recertification of a magnification scale standard it is necessary to
have an "independent source" determine if there has been any physical or
dimensional degradation of the standard which would raise questions about its
usefulness. When we recertify standards they are cleaned (using several
different methods) to remove physical debris including deposited oils from
scanning electron microscopes and re-measure them.

4) EDX standards are used for both energy and intensity calibration. I would
suggest that comment #2 above again applies. Please note that NIST has very
few appropriate (homogeneous to the micrometer level) standards. They include
some glasses (SRM 1872-3) and the Cu-Au (SRM-482) and Au-Ag (SRM-481) series.
NIST also has a publication (NBS 26028) on the proper preparation techniques
for SRM 481-2). The general point about traceable standards is that the
composition be known to a certain accuracy. As stated in ISO guide 25 the
accuracy (and measurement precision) must be better than needed for your
analysis.


Joe Geller
Geller MicroAnalytical Laboratory
426e Boston St.
Topfield, MA 01983-1212
508 887-7000, fax: 887-6671
geller-at-tiac.com

-Offering analytical services (SEM, EPMA, Auger, metallography, profilometry),
standards (magnification, elemental, compound, alloy, glasses, and minerals)
for microanalysis, and EPMA computer control systems (WDS and EDS).




From: paulc-at-arms.gps.caltech.edu (Paul K. Carpenter)
Date: Thu, 28 Mar 1996 10:33:05 -0800
Subject: Re: EMPA: anybody ever done hair?

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Message-Id: {v02110101ad808afb1a99-at-[131.215.67.110]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

John Fournelle wrote:

} Does anyone out there have any experience with EMPA of hair? Preparation,
} mounting, etc? Someone here is interested in looking at heavy metals that
} thru metabolism are concentrated in the hair.The levels will be low, so
} the MDL will be a question, but I don't know how the hair will stand up to
} an electron beam.

If you have enough material (hair, that is) it seems that XRF is a good
method. You could simply see what elements generate peaks, and then set up
for quantitative analysis. I suppose you could actually chop up the hair
samples to make a pressed XRF disk for quant analysis...

If you only have a few hairs and they must be analyzed as is, then EPMA is
going to be tough. You could try micro-XRF using a Mo thin foil mounted
above the sample; same comments as above for bulk XRF.

Paul


+------------------------------------------------------------+
| Paul K. Carpenter |
| Division Analytical Facility |
| Geological and Planetary Sciences MC 170-25 |
| California Institute of Technology |
| Pasadena, CA 91125 |
| 818-395-6126 (X-ray Lab) 818-568-0935 (FAX, Departmental) |
| paulc-at-arms.gps.caltech.edu |
+------------------------------------------------------------+






From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Thu, 28 Mar 1996 16:11:32 -0500 (EST)
Subject: Re: WORKING WITH PARAFFINIZED SECTIONS

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On Thu, 28 Mar 1996, Stanley Hayes wrote:

} I WOULD LIKE SOME INPUT FROM ANYONE WITH METHODS FOR DEALING WITH
} PARAFFINIZED SECTIONS NOW ON SLIDES AND TREATED FOR IN SITU
} HYBRIDIZATION AS WELL AS UNPARAFFINIZED SECTIONS. THE INVESTIGATOR
} WISHES TO IDENTIFY BY TEM THE TYPE OF CELLS LABELED. THANK YOU IN
} ADVANCE.
}
} S FRED. HAYES
}
Stan:

What I've done in the past for paraffinized sections is as follows:

Put slides in coplan jar and place in 60C oven............10 min.
Remove from oven and placr in xylene.......................3 min
xylene.......................3 min
100% ETOH....................3 min
95% ETOH....................3 min
80% ETOH....................3 min
70% ETOH....................5 min
50% ETOH....................5 min
DH2O...................2 X 10 min
2.5% Glut in PBS...........60 min
PBS....................2 X 15 min
1% OsO4 in PBS.............30 min
PBS....................3 X 15 min
50% ETOH...................10 min
70% ETOH...................10 min
95% ETOH...................10 min
100% ETOH...............3 X 10 min
Propylene Oxide..........2 X 10 min
1:1 PO:embedding resin..........60 min
1:2 PO:embedding resin..........60 min
1:3 PO:embedding resin..........60 min
100% embedding resin..........60 min

Before curing, drain off as much resin as possible, leaving a thin film.
Place a cured block on top of the area you want and cure for the
recommended time and temp for your resin.
Take off of slide by putting the slide on dry ice for a few minutes then
snap the block off. Section should come off of slide.

Hope this helps.

Peace,

Phil Rutledge
8-{)





From: Charles A. Garber :      103532.3325-at-CompuServe.COM
Date: 28 Mar 96 13:17:27 EST
Subject: Gold sputter targets

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On March 27, the following was posted:


Seeing Linda Fox's post just reminded me of something I found out the other
day here. Apparently the cheapest source of gold (99.9% pure) is new 'limited
edition' gold coins. Don't ask me why! One guy here had a crown about an inch
across which he then rolled to produce a 2 inch target for an industrial
coater. Saved the company a packet.

Richard Beanland
GMMTL Caswell,
Towcester,
Northants NN12 8EQ.

Tel. +44 1327 356363
Fax. +44 1327 356775
Email richard.beanland.gecm.com
==================================

For the almost twenty years that our firm has been supplying replacement sputter
coater gold targets, we have used the very highest gold starting purities
available, namely "four nines" or 99.99% pure gold.

Now I must start to wonder again, as I did twenty years ago, is such purity
really "necessary"? Clearly a lower level purity would be able to be offered at
a cheaper price.

The consensus that I seem to detect is that purity is very much on the minds of
the typical purchaser of a sputter coater cathode. The user wants reproducible
results and does not want to have stray elements present in the event EDS work
should be done on the now gold coated samples.

Also, as the impurity levels increase, assuming they are not precious metals but
are base metals and other elements, since such elements don't really sputter at
the typical voltages used in the sputter coaters typically found in an EM
laboratory, it is presumed that there is a "build up" of such impurity elements
on the target surface, reducing the sputtering rate, thereby increasing the
coating time, and exposing
the sample to far more electron and radiant heating than would otherwise be the
case.

Now I find it hard to believe that the typical user, with extremely costly
instrumentation, would be willing to take such risks to "save" such a small
monetary amount.

One reason why at least some purchased sputter coater cathodes appear expensive
is that the manufacturers supply them only as 3 mil or 5 mil thick targets. The
"fabrication" charge is the same whether for a thicker or thinner target. That
is why the SPI cathodes are all 10 mils thick, they last a lot longer and the
"fabrication" cost is a smaller percentage of the total cost or making the
target.

Disclosure: SPI Supplies manufactures gold and other replacement
targets for most manufacturers of sputter coaters and we would have an
obvious vested interest is not having SEM users rolling limited edition gold
coins into sputter coater targets!

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: em-at-mediacity.com (Ed Monberg)
Date: Thu, 28 Mar 1996 23:02:35 -0700
Subject: Re: Calibration Standards

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Message-Id: {v01540b02ad7fb91dc884-at-[205.216.172.10]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Yes, Good Morning !




An ISO 9000 slogan (to put on Watson's Forehaead):



Please rest assured that "ISO9000"

Implementation

will ensure that if you happen to make a poor product,

your product will be poor consistently.






Regards,



(signed) Ed Monberg {em-at-mediacity.com}

--------------------------------------------------

510-429-1060 Fax 429-1065
LMDC, (Laser Motion Development Co.)
3101 Whipple Road
Union City, CA 94587-1216


For our Most recent Catalogue of "On Hand" EQUIPMENT:
Send empty mail to: {Cat-at-lasermotion.com}


Our web page: http://www.lasermotion.com (Is beginning to take shape!)
Our e-mail: office-at-lasermotion.com

{-------------------------------- Our page width
-----------------------------}






From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Fri, 29 Mar 1996 07:11:10 -0500
Subject: Osmium tetroxide / IGSS

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Hello Everybody:

I've received several comments recently about the fate of silver-enhanced
gold particles (in EM) when specimens are treated with osmium tetroxide.
The literature indicates that this process can cause a slight reduction in
the size of the particles, probably due to re-oxidation of the deposited
silver. However, the effect seems to be very variable: in some cases many
of the particles disappear, or the color fades from the sections. Has
anyone had problems with this, and has anyone found a good way to prevent
it?

Thanks,

Rick Powell

*****************************************************************
* NANOPROBES, Incorporated *
* 25 East Loop Road, Suite 124, Stony Brook, NY 11790-3350, USA *
* Tel: (516) 444-8815 Fax: (516) 444-8816 *
* *
* http://www.tiac.net/users/everlast/nano *
*****************************************************************







From: Bo Johansen :      BOJ-at-bot.ku.dk
Date: Fri, 29 Mar 1996 15:17:42 GMT+0100
Subject: Thanks!!

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This list i amazing - I've got 32 answers to my question about Elmers glue.
Thanks to all of you.

Bo

_____________________________________________________________________
Bo Johansen E-Mail: BoJ-at-bot.ku.dk
Botanical Institute Vioce: +45 3532 2157
Gothersgade 140 FAX: +45 3313 9104
DK-1123 Copenhagen K, Denmark http://www.bot.ku.dk/www/staff/boj.htm
---------------------------------------------------------------------






From: chuck (c.p.t.) o'dale :      odale-at-bnr.ca
Date: Fri, 29 Mar 1996 09:18:00 -0500
Subject: SEM

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X400-Received:
by mta bnr.ca in /PRMD=BNR/ADMD=TELECOM.CANADA/C=CA/; Relayed; Fri, 29 Mar 1996 09:43:12 -0500
X400-Received:
by /PRMD=BNR/ADMD=TELECOM.CANADA/C=CA/; Relayed; Fri, 29 Mar 1996 09:18:38 -0500
X400-Received:
by /PRMD=BNR/ADMD=TELECOM.CANADA/C=CA/; Relayed; Fri, 29 Mar 1996 09:18:00 -0500
[/PRMD=BNR/ADMD=TELECOM.CANADA/C=CA/;bcars520.b.416:29.02.96.14.18.38]
X400-Content-Type: P2-1984 (2)
Content-Identifier: SEM

I received this address off the net RE: SEM.

Is this a news/exchange group?

My interests are in E-Beam testing, using SEM to probe functioning
integrated circuits to extract temporal and voltage information.

Chuck




From: Scott Holt :      102467.2752-at-CompuServe.COM
Date: 29 Mar 96 10:05:43 EST
Subject: TEM Epoxy: EPOTECH 353ND

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Hello all,

After the recent discussion of TEM epoxies, John Mardinly wrote to me requesting
any information on the toxic properties of Gatan's G1 Epoxy, as Gatan's MSDS
sheet did not mention any carcinogens. Since this is really Epoxy Technology's
EPOTECH 353ND, I will give the information I have regarding all-in-one.

Part B (hardener) of the 353ND product contains 0.05wt% of Acrylonitrile {CAS#
107-13-1, Toxicity Data: ACGIH(TLV-SKIN): TWA=2ppm}. Acrylonitrile appears on
the Mass Substance List. According to OSHA, acrylonitrile is a suspected cancer
causing agent.

The reason there is no indication of acrylonitrile on most MSDS sheets regarding
this product is that the quantities are below required reporting levels.
Imidazole is the only other hazardous ingredient in Part B of the epoxy, and
this should be reported on the MSDS due to significant levels.

For a copy of Epoxy Technology's MSDS sheet, please call them at (508)663-9782.

Hope this helps.

Best regards,
BUEHLER
41 Waukegan Rd.
Lake Bluff, IL 60044
(847)295-4546

BUEHLER WWW Page: http://www.buehlerltd.com






From: Alan Brooker :      alanb-at-jeolsys.demon.co.uk
Date: Fri, 29 Mar 1996 16:19:06 0000
Subject: Subscribe

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Please subscribe me!

Alan
*---------------------------------------*----------------------------------*
| Dr.Alan Brooker | Email: alanb-at-jeolsys.demon.co.uk |
| Product Support Manager | |
| JEOL (UK) Ltd., JEOL House, | Tel: +44 (0) 707 377117 |
| Watchmead, Welwyn Garden City, | Fax: +44 (0) 707 373254 |
| Herts, AL7 1LT, United Kingdom | |
*---------------------------------------*----------------------------------*





From: ayache-at-csnsm.in2p3.fr (Ayache Jeanne)
Date: Fri, 29 Mar 1996 18:49:21 +0000
Subject: inscription

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Message-Id: {199603291832.SAA129053-at-smtp-gw01.ny.us.ibm.net}

Dear colleague

I would like to belong to the users of the microscopy server.and receive
informations from microscopists.
I have a recent paper on preparation sample for TEM without ion milling :

Application of the ionless tripod polisher to the preparation of YBCO
superconducting multilayers and bulk ceramics thin films.
J.ayache and P.H Albar=E8de, Ultramicroscopy 60 (1995) 195-206

Best regards

Dr Jeanne Ayache

Centre de Spectroscopie Nucleaire et Spectrometrie de Masse
CSNSM -CNRS
Batiment 108
91405 Orsay Campus
=46rance
Tel :33 1 69 41 52 19
fax :33 1 69 41 52 68
Email : ayache -at-csn-hp.in2p3.fr






From: kna101-at-utdallas.edu
Date: Fri, 29 Mar 1996 08:41:37 -0600 (CST)
Subject: Re: WORKING WITH PARAFFINIZED SECTIONS

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
Content-Return: allowed
Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential

In my experience, the fixation quality of sections prepared for paraffin,
not to mention in situ hybrid., is too pour to get much informtion of
type of cell at EM level. But, I have reembedded tissue after removing
the media. The slides the tissue were on had been coated with gelatin
before the tissue was placed on them. I filled half a gelatin capsule with
new media and turned it on end, on the slide. Once the media had set up,
I pried the capsule off the slide. I could section the specimen this
way, but as I said the quality of the tissue was poor for EM. Good luck.

Karen Pawlowski

On Thu, 28 Mar 1996, Stanley Hayes wrote:

} I WOULD LIKE SOME INPUT FROM ANYONE WITH METHODS FOR DEALING WITH
} PARAFFINIZED SECTIONS NOW ON SLIDES AND TREATED FOR IN SITU
} HYBRIDIZATION AS WELL AS UNPARAFFINIZED SECTIONS. THE INVESTIGATOR
} WISHES TO IDENTIFY BY TEM THE TYPE OF CELLS LABELED. THANK YOU IN
} ADVANCE.
}
} S FRED. HAYES
}




From: Bruce Cutler :      BCutler-at-eureka.chem.ukans.edu
Date: Fri, 29 Mar 1996 15:47:26 -0500 (CDT)
Subject: Re: WORKING WITH PARAFFINIZED SECTIONS

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unsubscribe




From: Raymond F Egerton :      egerton-at-phys.ualberta.ca
Date: Fri, 29 Mar 1996 14:44:39 -0700 (MST)
Subject: Mike Whelan festschrift

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There will be a special issue of MICRON to honour Mike Whelan, one of the
pioneers of transmission electron microscopy in the physical sciences.

It will contain review and original-research papers written by scientists
who have worked in TEM imaging, defect analysis, electron diffraction and
electron energy-loss spectroscopy.

Manuscripts are due in September of this year. For further information,
contact Ray Egerton (egerton-at-phys.ualberta.ca).





From: Corvos-at-aol.com
Date: Fri, 29 Mar 1996 18:05:01 -0500
Subject: Re: Material Safety Data Sheets

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Scott Walck,

Every supplier must send a MSDS with all shippments under the DOT
regulations. And they must notify the shipping agent of the contents of every
box including the US postal service.

As an example: Even the smallest amount of oil, even in a pump it has to have
a MSDS when shipping. If the pump leaks and their is not a reference on the
out side of the package, in some states you must call in a HAZ-MAT team for
clean-up. It can be a very expencive package.

Walter Protheroe







From: Edmund Glaser :      eglaser-at-umabnet.ab.umd.edu
Date: Fri, 29 Mar 1996 10:40:47 -0500 (EST)
Subject: Re: Stereology

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On Tue, 26 Mar 1996 scott_l-at-unin1.unorth.ac.za wrote:

} Are there IBM compatible PC based programmes able to assist with doing
} stereology from TEM and LM micrographs. What are the hardware require-
} ments?
}
} Thank you
} Leon Scott
} SA
}

You should look into the recently released design based
stereology software, called Stereo Investigator. It's from
MicroBrightField in Colchester,VT, USA. Stereo Investigator
employs optical or physical disectors in conjunction with the
optical fractionator. This permits analysis of serial sections or
micrographs or live microscope images. The software includes 2D and 3D
mapping capabilities. It also lets you perform programmed scans of video
or optical images. The hardware requirements are basically those of a PC
with a video camera. MBF will give you specifics.

You can reach MicroBrightField at 802-655-9360 or by email:
info-at-microbrightfield.com.

They also have a web site: http://www.microbrightfield.com/microb

Edmund Glaser, D. Eng.
Dept. Physiol.
Univ. Md. School. Med.
Baltimore, MD 21201 USA
Ph: (410) 706-5041
Fax: (410) 706-8341





From: klivi-at-jhu.edu (Kenneth JT Livi)
Date: Fri, 29 Mar 1996 10:39:50 -0400 (EDT)
Subject: Re: Stereology

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Dear Microscopists,

Wil's recent comment on the safety hazards of distilled water brought to
mind some peculiar safety regulations here in MD. In reference to liquid N
(which can be dangerous stuff), we were first required to wear gloves while
handling the stuff. Then came goggles and soon after that, full face
shields. The funny thing is, the most dangerous aspect of our handling of
LN2 is that students often wear sandals in the summer and are very likely
to get stung by droplets. There are no safety measures for feet
protection! But now that I have mentioned it, some occupational safety
officer listening in will recommend new safety procedures requiring
protective booties!
In the end, we can't legislate common sense, nor can we abdicate
responsibility to those above.

(The opinions above are mine and of anyone who agrees with them.)

Kenneth JT Livi
Department of Earth and Planetary Sciences
34th and Charles Streets
The Johns Hopkins University
Baltimore, Maryland 21218
(410) 516-8342 (voice)
(410) 516-7933 (fax)
klivi-at-jhu.edu (e-mail)






From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Fri, 29 Mar 1996 18:16:26 -0400 (EDT)
Subject: RE: Looking for the reference in full by Luft J.H., 1966

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X-NUPop-Charset: English

In message Fri, 29 Mar 96 19:31:57 -0800,
"Alberto J. Villena" {villena-at-ibm.net} writes:

} Dear colleagues,
}
} I'm lookink for the following reference in full:
}
} } Luft J.H., 1966: Fed. Proc, 25 1773
----------------

Here is the full reference:

Luft, J.H. 1966. Fine structure of capillary and endocapillary layer as
revealed by ruthenium red. Fed. Proc., 25:1773.

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology &
Director, Cornell Integrated Microscopy Center
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: Scott Holt :      102467.2752-at-CompuServe.COM
Date: 29 Mar 96 13:54:03 EST
Subject: TEM Epoxy: EPOTECH 353ND

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Hello all,

After the recent discussion of TEM epoxies, John Mardinly wrote to me requesting
any information on the toxic properties of Gatan's G1 Epoxy, as Gatan's MSDS
sheet did not mention any carcinogens. Since this is really Epoxy Technology's
EPOTECH 353ND, I will give the information I have regarding all-in-one.

Part B (hardener) of the 353ND product contains 0.05wt% of Acrylonitrile {CAS#
107-13-1, Toxicity Data: ACGIH(TLV-SKIN): TWA=2ppm}. Acrylonitrile appears on
the Mass Substance List. According to OSHA, acrylonitrile is a suspected cancer
causing agent.

The reason there is no indication of acrylonitrile on most MSDS sheets regarding
this product is that the quantities are below required reporting levels.
Imidazole is the only other hazardous ingredient in Part B of the epoxy, and
this should be reported on the MSDS due to significant levels.

For a copy of Epoxy Technology's MSDS sheet, please call them at (508)663-9782.

Hope this helps.

Best regards,
BUEHLER
41 Waukegan Rd.
Lake Bluff, IL 60044
(847)295-4546





From: Daniel L. Callahan :      dlc-at-owlnet.rice.edu
Date: Fri, 29 Mar 1996 15:37:15 -0600 (CST)
Subject: epoxies and confidentiality

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Good Afternoon:

A large number of microscopists in the hard sciences and electronics
industry use epoxies like Epoxy Technology 353ND or Gatan G-1 (which may
or may not be the same thing). Since epoxies are basically commodities,
these companies have a vested interest in not revealing their sources and
compositions. However, as a physical scientist, sometimes it behooves me
to know the chemicals I am using in the experiment. Basically, I would
like to know the composition of Epo Tech 353ND sufficiently to be able to
determine potential sample interactions and health risks without
revealing sufficient information to readily reproduce the product. I
don't know if this is possible: it may be that anyone knowing the secret
ingredients can easily determine their proportion and processing. On the
other hand, it may be that any competent polymer chemist could guess the
composition reasonably accurately.

I would particularly appreciate commentary from the business providers
who watch the list. Incidentally, if you do know the composition please
do NOT post it directly to the list unless you know that it has been
published or patented (which is also public documentation). This is
someone's business interest.


Daniel L. Callahan
Department of Mechanical Engg. and Materials Science
Rice University
dlc-at-owlnet.rice.edu




From: Dr. L. P. Stoter :      LPS-at-teknesis.demon.co.uk
Date: Sat, 30 Mar 1996 07:57:02 +0000
Subject: Re: Safety & Liquid N2

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X-Sender: (Unverified)
Message-Id: {v01510104ad82961fa812-at-[158.152.199.245]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Wil's recent comment on the safety hazards of distilled water brought to
} mind some peculiar safety regulations here in MD. In reference to liquid N
....
} officer listening in will recommend new safety procedures requiring
} protective booties!
} In the end, we can't legislate common sense, nor can we abdicate
} responsibility to those above.

Try getting your safety officer to conduct an experiment:

1. Hold out hand,
2. Pour a small volume of liquid N2 over hand
3. Now the interesting bit - put on a glove, and pour the same quantity of
liquid N2 into glove.
4. Phone for ambulance.

The point is that a brief contact causes no problems, but if the contact is
continued you get a nasty burn.

Gloves, goggles, masks (and shoes) are actualy more dangerous when handling
liquid N2 than sandles and no protection. And clothes are actually more
dangerous than being naked. Get the safety officer to experiment. With a
little persuasion you can probably convince the safety officer that when
handling liquid N2, everbody should be naked.

More seriously, bureaucrats, administrators and the inexperienced should
talk to somebody who has real knowledge.

--------------------------------------------------------------
Dr. Larry Stoter
Technesis
17, Rocks Park Road, Uckfield, E. Sussex, TN22 2AT, United Kingdom
Larry-at-teknesis.demon.co.uk
--------------------------------------------------------------






From: Finn-Mogens Haug :      f.m.s.haug-at-basalmed.uio.no
Date: Sat, 30 Mar 1996 12:02:32 +0100
Subject: Re: Stereology

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199603301051.LAA15533-at-pons.uio.no}
X-Sender: finnmog-at-pons.uio.no
X-Mailer: Windows Eudora Pro Version 2.1.2
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

On Tue, 26 Mar 1996 scott_l-at-unin1.unorth.ac.za wrote:
}
} Are there IBM compatible PC based programmes able to assist with doing
} stereology from TEM and LM micrographs. What are the hardware require-
} ments?
}
} Thank you
} Leon Scott
} SA
}

I did not see anyone mention:

Kinetic Imaging Ltd.,
South Harrington Bldg
Sefton Street
Liverpool L3 4BQ
UK
Tel +44(0) 151 709 8661
Fax +44(0) 151 709 8633
E-mail: kineticimage-at-aol.com

They offer "Digital Stereology", running under DOS/Windows-3, HW requirements
486/33 MHz, 8MB RAM, 200MB Hard disk, High resolution monitor.
Imaging board by Matrox or Univision. CCD-camera. It is not quite clear
whether you
also need to purchase their "Fenestra" image processing package.

I suppose many would wish a stereology subsystem to behave as an add-on to
whater
image processing system (IPS) he or she is already used to. This might take
the form of
an "autonomous" system running on a PC with only a high resolution graphics
display board
and sufficiently processing power and RAM (and capable of dealing with
TIFF-images).
Or it might take the form of a function library which could integrated into
any IPS capable
of linking dll libraries (such as Image Pro Plus, Optimas, analySIS,
Visilog, to mention a
few IPS's which I believe have this capability).

So far the range of stereology software seems to be much smaller than that
of general
image analysis systems. However, with the increasing use of digital images,
there
should be room for many such products. Any more feedback to the original
query????

Best regards
*****************************************************************
Finn-Mogens Haug
University of Oslo, Institute of basic medical sciences,
Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Box 1105 Blindern Phone : +47 22 85 12 67
N-0317 Oslo, NORWAY Fax : +47 22 85 12 78
*****************************************************************






From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Sat, 30 Mar 1996 19:00:21 -0600
Subject: Re: LN2 safety (was no subject)

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} Dear Microscopists,
}
} Wil's recent comment on the safety hazards of distilled water brought to
} mind some peculiar safety regulations here in MD. In reference to liquid N
} (which can be dangerous stuff), we were first required to wear gloves while
} handling the stuff. Then came goggles and soon after that, full face
} shields. The funny thing is, the most dangerous aspect of our handling of
} LN2 is that students often wear sandals in the summer and are very likely
} to get stung by droplets. There are no safety measures for feet
} protection! But now that I have mentioned it, some occupational safety
} officer listening in will recommend new safety procedures requiring
} protective booties!
} In the end, we can't legislate common sense, nor can we abdicate
} responsibility to those above.
}
} (The opinions above are mine and of anyone who agrees with them.)
}
} Kenneth JT Livi

Kenneth:
Your students are correct, it's the safety officers who are in
error. Any good safety manual for cryogenic gases specifically recommends
sandals, and *against* shoes, gloves, and the like.
The reason is the Leidenfrost effect. The LN2 droplets
flash-evaporate when they hit bare skin, producing an insulating layer of
gas, and prevents freezing damage. Assuming it's a quick spill, and not
continous contact. Shoes, glove (including asbestos gloves), and any other
clothing that confines the LN2, and cold gas *will* cause freezing.
A face shield is a good measure, byt the gloves and any other
similar regs are dangerous.
Phil

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: em-at-mediacity.com (Ed Monberg)
Date: Sat, 30 Mar 1996 22:38:16 -0700
Subject: Re: LN2 safety (was no subject)

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Message-Id: {v01540b14ad83c6ff379c-at-[205.216.172.10]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

As a wild and fun loving farm boy who went into science, I found LN2 to be
a wonderful toy. The closest I ever came to danger was when an associate
put some in a nalgene bottle and closed the top, resulting in a frozen
plastic flask going "POP".

We did stunts along the lines of freezing the contents of our associates
lunch pails, shattering a fish (boy was that an ultimately smelly mistake
!), and once when we froze a rubber tube in the neck of a dewar by mistake,
we had to empty the entire 50 liters into a trash container.

Consider the heat of evaporation, compare it to the heat of condensation of
steam, and think how many burns YOU personally know of from that source vs.
from LN2.

I am repelled at the lack of savy and the excess of fear instilled by the
law ("profession") regarding such pseudo threats as LN2.

The presumptive (and ignorant) reaction is irrational, but accfording to
the same principles, TWO safety officers should come in the box containing
each microwave oven, a gun should bear a judge attached, and an auto, a
member of the supreme court, or a receipt for $1000 from your favorite
lobbyist. Remember, not only can the law be bought, It's really CHEAP !


!!!!!!


Good luck. Just act normal.





Regards,



(signed) Ed Monberg {em-at-mediacity.com}

--------------------------------------------------

510-429-1060 Fax 429-1065
LMDC, (Laser Motion Development Co.)
3101 Whipple Road
Union City, CA 94587-1216


For our Most recent Catalogue of "On Hand" EQUIPMENT:
Send empty mail to: {Cat-at-lasermotion.com}


Our web page: http://www.lasermotion.com (Is beginning to take shape!)
Our e-mail: office-at-lasermotion.com

{-------------------------------- Our page width
-----------------------------}






From: Robert.R.Wise-at-Sparc5.Microscopy.Com
Date: Sun, 31 Mar 1996 11:02:41 +0000
Subject: modems

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Although not strictly a microscopy question, the members of this
list seem to know a lot about computers as well. So here goes...

Does anyone have any recommendations on the proper modem for
connecting a Macintosh (Centris 610 with EtherNet card) at home to a local
phone line? US Robotics has a 28.8 kbps model for about $200. What does
the 28.8 stand for? Is this just a plug in or do I have to get something
like an adapter (such as an Asante' FriendlyNet Media Adapter)? Please
help the computer illiterate.

Bob Wise






From: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 31 Mar 1996 12:41:20 -0600
Subject: Submission of Papers for the 96 MSA/MAS/MSC-SMC Meeting

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SUBJECT: Submission of Papers for the 96 MSA/MAS/MSC-SMC Meeting

********************************
* *
* MICROSCOPY & MICROANALYSIS -96 *
* Joint Annual Meeting of *
* MSA/MAS/MSC-SMC *
* *
********************************


SPECIAL EXTENSION FOR RECEIPT OF ABSTRACTS


Some microscopists and microanalyists who wished to present papers at
Microscopy & Microanalysis '96 did not receive their Registration Bulletin /
Call for Papers package in time to submit by the published deadline (March
15). Because of this, a SPECIAL ARRANGEMENT has been made to allow papers
to still be accepted until April 10th 1996 (receipt of manuscript at the
address listed below). If you wish to submit but have not, please contact
the Business Office (BusinessOffice-at-MSA.Microscopy.Com 1-800-538-3672)
as soon as possible, for any additional details or questions on when and
where to send
your paper.

You may download all of the submission information INCLUDING forms
and instructions from the Web at:

http://www.msa.microscopy.com


At this late date, please mail copies directly as follows:


Original & 1 Copy:

Microscopy & Microanalysis-96
4 Barlows Landing Rd.
Suite 8
Pocasset, Ma. 02559 USA.



2 Copies:

Dr. Nestor J. Zaluzec
Microscopy & Microanalysis-96
797 Bonnie Brae Ct.
Bolingbrook, Illinois 60440 USA



-----------------------------------------------------------------------







From: rw9-at-psu.edu (Rosemary A. Walsh)
Date: Sun, 31 Mar 1996 21:24:23 GMT
Subject: Re: EM User Fees

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Microscopy-at-Sparc5.Microscopy.Com (MSA Listserver)

At 9:52 AM 3/28/96 -0500, Hand,Arthur wrote:
} Since there have been a large number of requests for the data on user fees, I
} have decided to post the information on the listserver as attachments to
} this message. They were created in MS Word 6; one attachment is in Mac
} format; the other is in PC format. If you have problems opening them, let me
} know and I will fax the data to you as soon as possible.
}
} Arthur R. Hand
} Central EM Facility
} UConn Health
} Ctr
}
} Content-Type: application/octet-stream; name="Fee Comparison-Mac"
} Content-Transfer-Encoding: x-uuencode
}
} Attachment converted: MacintoshHD:Fee Comparison-Mac (????/----) (0000215E)
} Content-Type: application/octet-stream; name="Fee Comparison-PC"
} Content-Transfer-Encoding: x-uuencode
}
} Attachment converted: MacintoshHD:Fee Comparison-PC (????/----) (0000215F)


Arthur,
Thanks for your time and effort--I had a problem opening the Mac file.
Would you please fax me the file? My FAX number is 814-863-1357.

Rosemary Walsh
EM Facility
The Biotechnology Institute
519 Wartik Lab
The Pennsylvania State University
University Park, PA 16802






From: Robert.R.Wise-at-Sparc5.Microscopy.Com
Date: Sun, 31 Mar 1996 15:56:57 +0000
Subject: LN Dewar

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I need to purchase a 10 liter Dewar for LN transfer and storage. Does
anyone have any recommendations for an appropriate product? I would like
to find one with rate of boil-off of less than one liter per day (such
specs are rarely given in catalogs).

Thanks in advance,

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: Finn-Mogens Haug :      f.m.s.haug-at-basalmed.uio.no
Date: Sun, 31 Mar 1996 22:28:10 +0100
Subject: Re Stereology (re-posting)

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199603312117.XAA22651-at-pons.uio.no}
X-Sender: finnmog-at-pons.uio.no
X-Mailer: Windows Eudora Pro Version 2.1.2
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I take the liberty of re-posting this message, which had
a terrible layout. Also, the e-mail address has been verified
and seems to work.

On Tue, 26 Mar 1996 scott_l-at-unin1.unorth.ac.za wrote:
}
} Are there IBM compatible PC based programmes able to assist with doing
} stereology from TEM and LM micrographs. What are the hardware require-
} ments?
}
} Thank you
} Leon Scott
} SA
}

I did not see anyone mention:

Kinetic Imaging Ltd.,
South Harrington Bldg
Sefton Street
Liverpool L3 4BQ
UK
Tel +44(0) 151 709 8661
Fax +44(0) 151 709 8633
E-mail: kinetimage-at-aol.com (or Kinetimage-at-aol.com)

They offer "Digital Stereology", running under DOS/
Windows-3, HW requirements 486/33 MHz, 8MB RAM, 200MB
Hard disk, High resolution monitor. Imaging board by
Matrox or Univision. CCD-camera. It is not quite clear
whether you also need to purchase their "Fenestra" image
processing package.

I suppose many would wish a stereology subsystem to behave
as an add-on to whatever image processing system (IPS) he
or she is already used to.

It might take the form of an "autonomous" system running
on a PC with only a high resolution graphics display board
and sufficient processing power and RAM (capable of dealing
with TIFF-images). Or a function library which could
be integrated into any IPS capable of linking dll libraries
(such as Image Pro Plus, Optimas, analySIS, Visilog, to
mention some which I believe have this capability).

With the increasing use of digital images, there should be
room for many such products. Any more feedback to the
original query?

Best regards






******************************************************************
Finn-Mogens Haug
University of Oslo, Institute of basic medical sciences,
Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Box 1105 Blindern Phone : +47 22 85 12 67
N-0317 Oslo, NORWAY Fax : +47 22 85 12 78







From: Hand-at-nso1.uchc.edu (Hand,Arthur)
Date: Thu, 28 Mar 1996 09:52:35 -0500
Subject: EM User Fees

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From: Hand-at-nso1.uchc.edu (Hand,Arthur)
Date: Thu, 28 Mar 1996 09:52:35 -0500
Subject: EM User Fees

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From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 01 Apr 96 09:16:15 EST
Subject: Re: re: safety in EM labs

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Dear Ken,
You're right about not being able to legislate common sense. In the case of
liquid nitrogen, I have seen LN2 slopping out of styrofoam cups onto peoples'
clothes, into instruments, all over papers, etc.
For all of the examples everyone can cite of *unnecessary* regulations, I am
still amazed to see kitchen microwave ovens in labs being used to heat toxic
chemicals, including fixatives and heavy metals, the fumes from which then
"ventilate" into the open lab when the door is opened. In some cases, the oven
is then used to heat coffee and/or lunch.
Steven Slap
75767,640-at-compuserve.com





From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 1 Apr 1996 11:02:49 -0500 (EST)
Subject: Re: Safety & Liquid N2

Contents Retrieved from Microscopy Listserver Archives
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} Gloves, goggles, masks (and shoes) are actualy more dangerous when handling
} liquid N2 than sandles and no protection.

True with the exception that the shutoff valve handle on a big
LN2 tank will get cold enough to be dangerous, and does not exhibit the
leidenfrost effect. Using a glove or other insulation when turning off
the LN2 after filling the dewar makes good sense.
}
} More seriously, bureaucrats, administrators and the inexperienced should
} talk to somebody who has real knowledge.
}
A great general rule. Reading the manual also helps, and every
lab should have a safety manual on hand.
Yours,
Bill Tivol




From: Kovacs Arpad :      femkov-at-gold.uni-miskolc.hu
Date: Mon, 1 Apr 1996 17:39:29 GMT
Subject: Re: Safety & Liquid N2

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From: Weiming Yu :      weiming-at-LFD.physics.uiuc.edu
Date: Mon, 1 Apr 1996 12:15:03 -0600 (CST)
Subject: Temperature controller

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Dear Friends:

Does anyone know which company sells temperature controller for optical
microscope (specially for old immersion objective) ?
Thank you.

Weiming Yu, Ph.D.
Dept. of Physics, UIUC
1110 W. Green
Urbana, IL 61801
weiming-at-lfd.physics.uiuc.edu





From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Mon, 1 Apr 1996 13:16:16 -0500
Subject: HRP substrates

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Message-Id: {v01520d04ad85ccdb2569-at-[128.206.15.189]}
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I plan to return to some HRP-based histochemistry and thought I
would stir up some controversy by asking what you all feel the best
precipitating substrate for immunocytochemistry with peroxidase labels. I
have always used DAB without metals. What is the disadvantage of using
metal intensification? Does anybody have experience with the "stable"
solution of DAB (e.g., Pierce Chemicals Metal Enhanced DAB Substrate Kit)?
Are they worth the convenience? TIA.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Dave King (607)757-1248 T37/257-3 Ext DEKING-at-VNET.IBM.COM
Date: 1 Apr 1996 10:08:33 EST
Subject: Re: EM User Fees

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Message-Id: {199604011506.JAA12875-at-Sparc5.Microscopy.Com}

To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}
*** Reply to note of 04/01/96 07:19

We do outside analytical work over the complete spectrum of
materials, chem lab and product assurance areas. We charge
different rates for each instrument and basically different
operations. Sample prep, data analysis, and report writing get
charged, as well as actual SEM time, for example.

We figure it as "beginning to end." Think about how much time
you'd have saved if you didn't have that job at all. Everything
must be covered, or someone else is subsidizing it.

. {
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: mlamvik-at-mcnc.org
Date: Mon, 1 Apr 1996 16:28:54 -0500
Subject: Getting data from Kevex 8000?

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Does anyone know how to get spectrum data out of a Kevex 8000 analyzer into
a desktop computer for further analysis? Advice will be appreciated.

Thanks,
Michael Lamvik
MCNC, Research Triangle Park, North Carolina






From: Richard Thrift :      Richard_Thrift-at-depotech.com
Date: Mon, 01 Apr 1996 11:44:05 -0800
Subject: Re: microscope safety

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Message-Id: {s15fc16b.056-at-depotech.com}
X-Mailer: Novell GroupWise 4.1

I have to tell you about this: after reading the mail on this topic I used the
far door to enter our microscope room. I rarely use this door. It has a
sign (which I've never noticed before; it must have been there for the last
half-year) saying "NOTICE Safety glasses required in this area"

Richard

} } } Kenneth JT Livi {klivi-at-jhu.edu} 03/29/96 06:39am } } }
Dear Microscopists,

Wil's recent comment on the safety hazards of distilled water brought to
mind some peculiar safety regulations here in MD. In reference to liquid N
(which can be dangerous stuff), we were first required to wear gloves
while handling the stuff. Then came goggles and soon after that, full face
shields. The funny thing is, the most dangerous aspect of our handling of
LN2 is that students often wear sandals in the summer and are very likely
to get stung by droplets. There are no safety measures for feet
protection! But now that I have mentioned it, some occupational safety
officer listening in will recommend new safety procedures requiring
protective booties!
In the end, we can't legislate common sense, nor can we abdicate
responsibility to those above.

(The opinions above are mine and of anyone who agrees with them.)

Kenneth JT Livi
Department of Earth and Planetary Sciences
34th and Charles Streets
The Johns Hopkins University
Baltimore, Maryland 21218
(410) 516-8342 (voice)
(410) 516-7933 (fax) klivi-at-jhu.edu (e-mail)








From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Mon, 01 Apr 1996 09:12:22 -0600
Subject: Re: EM User Fees

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We had momentary problems with the files on a Mac. We first had to locate
them, our Eudora didn't tell us which folder it dropped it in. It ended up
in SYSTEM\EUDORA FOLDER\ATTACHMENTS, which we should have guessed.

We could not simply double-click on it to open it. Apparently it did not
have the extra information to tell the desktop what kind of file it was.
Therefore, we had to first open up Word, then open the document the
old-fashioned way through the FILE/OPEN menu. I have found that this is
often required and usually works for documents that come through different
channels.

Being a Windows man myself, I am used to opening up Word and leaving it
running throughout the day so it is a simple matter to switch to Word and
open a new document through the pull-down menus. Sometimes the normal
associations get lost or naming conventions are not followed. But the
pull-dopwns almost always work.

At 09:24 PM 3/31/96 +0000, you wrote:
} Arthur,
} Thanks for your time and effort--I had a problem opening the Mac file.
} Would you please fax me the file? My FAX number is 814-863-1357.
}
} Rosemary Walsh
} EM Facility
} The Biotechnology Institute
} 519 Wartik Lab
} The Pennsylvania State University
} University Park, PA 16802
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Mon, 1 Apr 1996 16:29:16 +0000 (GMT)
Subject: Re: C2 movement

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Microscopy List {Microscopy-at-Sparc5.Microscopy.Com}


To Jan van der Meijden

thank you for your mail,

I just checked what you recommend. The displacement is about 20 mm, so
within your specifications. Despite it is within Philips specification, this
does not allow to work easily from bright field-dark field and
diffraction modes, because when working at about 100 000 times (usual
magnification rate in order to make BF DF diff images), the movement
comes to be 20*20= 400 mm, which is over my own specifications (i.e. the
light goes out of the screen therefore I have to get back to 20 000
magnification in order to see where it is...).

The only reasonable way I have found is to press the intensity limit
softkey when being near the cross over in order to prevent from going too
low in intensity of the C2 lens. This works, but I will welcome with
great pleasure another method to get rid of the problem.

On the other hand, during the alignment procedure, due to this shift
problem, we do not know exactly how to center correcty the light. I am
used to press at each step the normalization button (i.e. photo button),
but am amazed because after the whole process (canon alignment) usually
the light does NOT get back in the center of the screen. Well maybe it is
within specifications, I haven't checked this out.

Thank you for every new input, I am sure that many Philips microscope users
are eager to know more about the subject. For this reason I an sending a
copy to the list as well.

Sincerely,


Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Luis Sole i Sabaris
E-08028 BARCELONA

Tel +34 3 402 16 95
Fax +34 3 402 13 98

On Thu, 28 Mar 1996, Jan van der Meijden wrote:

} Dear Sir
}
} Via a detour, your request to know the C2 movement when changing C2
} has arrived on my desk.
} The procedure we use in the factory is as follows:
} Magnification 5800x
} Focus C2 and centre the beam.
} Fully over focus the beam and focus again. Centre the beam again
} Fully underfocus the beam and focus again. Measure the distance from
} the centre. This distance should be less than 5cm.
} According to your mail this distance on your scope is about 7cm at
} 20.000x and thus far within specification.
} The problem is caused by the high remanence in the C2 lens and the C2
} lens not perfectly mechanically aligned in the optical axis.
} To overcome the problem use one spotsize higher or lower in order to
} do a light normalisation.
}
} Regards Jan van der Meijden
}
}
}
}
} Yours sincerely
}
}
}
} Jan van der Meijden
}




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Tue, 2 Apr 1996 13:20:10 +1100
Subject: EM: Marine invertebrate texts

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Dear Microscopists,
Does anyone out there know of a textbook or any other reference material
which covers integumental ultrastructure - cuticle, epithelium and
associated structures and also oogenesis - in marine invertebrates. We have
a marine science student who is studying subclass Ascothoracida who is
having problems getting the ultrastructure info she needs.

Thanks in advance.

Richard


Richard Easingwood
South Campus Electron Microscope Unit
School of Medical Sciences
University of Otago
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD















From: rh208-at-cus.cam.ac.uk (Ray Hicks)
Date: Mon, 1 Apr 1996 15:47:11 +0000
Subject: Re: Microscope Incubators

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Hi Brian,
I think that most CO2 sensors work by measuring the thermal conductivity or
indirectly the heat capacity of the gas mix, this makes them awkward to use
in a situation where relative humidity and temperature may fluctuate (since
both of these will affect the temperature of the sensor). They also might
(I don't know) need a large volume of gas to pass over them to register a
change.
I would suggest that you might look into three alternative strategies

i) the simplest is to use a cannister of premixed gas, presuming that you
want to have a constant pCO2.

ii)Monitor the pH of the medium colorimetrically, assuming the colour
change of the medium is mainly due to dissolved CO2, which may not be the
case.

iii) Measure the infra-red absorbance of the gas mix, I believe that this
method is used in some incubators, but I'm not sure what wavelength to use.


(i) is the easiest and cheapest assuming you don't want to vary the pCO2,
if you did you could adapt it by using two bottles, one of CO2 and one of
air, these methods might cause humidity regulation problems. (ii) would
have to be verified, and if it worked you'd have to build your own probe
(I'd have a go if you like), but you would have the benefit of regulating
dissolved CO2 not just gaseous. Depending on the absorption spectrum of
CO2, it might be hard to find suitable detectors and emitters for (iii).

I hope this helps

Ray

At 2:13 pm 29/3/96, Brian Burgess wrote:
} Hi Everyone
} We are designing an incubator to fit on a microscope to set the
} temperature at 37C and control humidity and maintain a level of
} CO2 at 5%. I have had a real hard time locating a CO2
} control/sensing unit. I did find one marketed by Forma
} Scientific but it is lunky and quite large. I rather need a
} smaller unit. As mentioned before I need to control temperature
} and humidity levels. Does anyone know of a good system or a
} manufacturer of such a system?

} ----------------------------------------------
} Brian Burgess
} Chemical Engineering
} University of Florida
} e-mail: burgbr-at-che.ufl.edu

Ray Hicks
________________________________________________________________________
|University of Cambridge |Tel 01223 330149 |
|Department of Medicine |Fax 01223 336846 |
|Level 5, Addenbrookes Hospital |e-mail rh208-at-cus.cam.ac.uk |
|Hills Road Cambridge |Web Page/ facsmac.med.cam.ac.uk |
|CB2 |ftp server 131.111.80.78 |
|UK | |
|_________________________________|_____________________________________|






From: !Microscopy-request-at-Sparc5.Microscopy.Com (Robert Kayton,MAC,CROET)
Date: Mon Mar 18 12:38:43 -0500 1996
Subject: Re: Microscope Incubators

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Sender: kayton-at-ohsu.edu (Robert Kayton)
Message-Version: 2
} To: microscopy-at-Sparc5.Microscopy.Com (!Microscopy)

Title: Biological Science Technician (Plants)
Lab: USDA-ARS, Salinas, CA

The USDA-ARS is seeking a biological science technician (plants)
(GS-404-7, 8, or 9) for the Crop Improvement and Protection Research Unit
in Salinas, CA. The incumbent will share responsibilities in electron
microscopy of plant virus infections for ultrastructural characteristics.
The incumbent will also assist in research involving molecular, serological,
and biological studies of several plant viruses infecting sugarbeet and
vegetables. Candidate must have a knowledge of electron microscopy,
plant virology, and knowledge of microbiological techniques. Must be a U.S.
citizen. Bachelors degree is desirable. Salary is commensurate with
experience ($24,610-39,140 per annum).
For information regarding research program contact Gail C. Wisler or
James E. Duffus (408)755-2835. For information regarding application
procedures/forms contact Tom Nelson (408)755-2810. Applications must be
postmarked by May 6, 1996. The USDA is an equal opportunity employer.





From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 01 Apr 96 09:16:50 EST
Subject: Re: LN2 Dewar

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Bob-
Energy Beam Sciences offers a range of stainless steel LN2 lab containers, from
5L up to 220L. The 10L container has a loss rate of .8 liters per day. Please
contact me directly by e-mail or telephone (800-992-9037) for part numbers,
prices, etc.
Steven E. Slap, Vice-President
75767,640-at-compuserve.com





From: kna101-at-utdallas.edu
Date: Mon, 1 Apr 1996 07:55:16 -0600 (CST)
Subject: Re: your mail

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Dear Kenneth:

I understand your frustration with all the regulations that just keep
coming for hazardous material in the work place, but I have to point out
the flip side of this issue. I know of a lab that recently got into
trouble because for years the regulators were leaving it up to the head
of the lab to make sure precautions were taken. This lab was solely made
up of students and the professor/lab head. The professor was leaving
everything up to the students to do and learn, giving little if any
dirrection to them. These students came from accounting and psycology
and english-major backgrounds. They had no experience working in an
environment full of hazardous substances, such as a histology lab. They
were working with flourescent microscopy, without using the proper
filters and hazardous substances without the proper protection, ie
gloves, fume hoods. All waste was going down the drain! This is an
EXTREME example, I know. I just wanted to show you how bad it can get if
some sort of overseeing isn't in place.

I've worked in histology labs
for 16 years and I do get tired of having the rules changing about once a
year, ie- we have a built-in book self near a fire door. It's been there
3-4 years. Last month the inspectors came through and told us to take it
down because it's too close to the door. They've inspected that bookcase
every year and not until now was it a problem! So I do know how you feel.

Karen Pawlowski

On Fri, 29 Mar 1996, Kenneth JT Livi wrote:

} Dear Microscopists,
}
} Wil's recent comment on the safety hazards of distilled water brought to
} mind some peculiar safety regulations here in MD. In reference to liquid N
} (which can be dangerous stuff), we were first required to wear gloves while
} handling the stuff. Then came goggles and soon after that, full face
} shields. The funny thing is, the most dangerous aspect of our handling of
} LN2 is that students often wear sandals in the summer and are very likely
} to get stung by droplets. There are no safety measures for feet
} protection! But now that I have mentioned it, some occupational safety
} officer listening in will recommend new safety procedures requiring
} protective booties!
} In the end, we can't legislate common sense, nor can we abdicate
} responsibility to those above.
}
} (The opinions above are mine and of anyone who agrees with them.)
}
} Kenneth JT Livi
} Department of Earth and Planetary Sciences
} 34th and Charles Streets
} The Johns Hopkins University
} Baltimore, Maryland 21218
} (410) 516-8342 (voice)
} (410) 516-7933 (fax)
} klivi-at-jhu.edu (e-mail)
}
}
}




From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Mon, 1 Apr 1996 05:57:14 -0600
Subject: Re: EM User Fees

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Microscopy-at-Sparc5.Microscopy.Com

I have a general question about user fees, in particular how various people
bill for hours. We use a "gentle" system with a computer interfaced to the
microscope to track real hours, rather than a harsh system where one bills
from the minute that the user walks in the door. As a consequence, we have
comparatively low hours/week, and I have recently been criticized for this.
I would be very interested in comments both from users and administrators
about this - should TEM facilities bill like lawyers or not?




From: ychen-at-MACC.WISC.EDU
Date: Sun, 31 Mar 1996 23:33:15 -0600
Subject: Re: modems

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} Date: Sun, 31 Mar 1996 11:02:41 +0000
} From: Robert.R.Wise-at-Sparc5.Microscopy.Com
} Subject: modems
} To: Microscopy-at-Sparc5.Microscopy.Com
} X-Sender: wise-at-vaxa.cis.uwosh.edu
} MIME-version: 1.0
} Content-type: text/plain; charset="us-ascii"
} Content-transfer-encoding: 7BIT
}
} Although not strictly a microscopy question, the members of this
} list seem to know a lot about computers as well. So here goes...
}
} Does anyone have any recommendations on the proper modem for
} connecting a Macintosh (Centris 610 with EtherNet card) at home to a local
} phone line? US Robotics has a 28.8 kbps model for about $200. What does
} the 28.8 stand for? Is this just a plug in or do I have to get something
} like an adapter (such as an Asante' FriendlyNet Media Adapter)? Please
} help the computer illiterate.
}
} Bob Wise


Bob,
The speed of a modem is measured by Bit per Second (bps). The 28.8kbps
modem will double the transmission speed than the 14.4 kbps one. The 28.8k
modem uses error correction and data compression protocols called v.42bis,
MNP2-5 for high speed data transmission. The installation is simple: just
plug the modem cable (suppose this is a Mac modem) in the serial port
marked as MODEM on your Mac, and connect phone line. You need to set up or
configurate your communication software before start. EtherNet card is
used for network connection, not for modem. If you have any question,
please contact me. Good luck.
Ya Chen


Ya Chen

Integrated Microscopy Resource (IMR)-- III M M RRRRRR

an NIH Biomedical Research Resource I M M M M R R
University of Wisconsin, Madison, WI I M M M RRRRRR
1675 Observatory Drive #167 I M M R R
Madison, WI 53706 I M M R R
TEL : 608-263-8481 I M M R R

FAX : 608-265-4076 III M M R R

Email:YChen-at-macc.wisc.edu
IMR WWW Home Page: http://www.bocklabs.wisc.edu/imr.html
2nd Symposium on Integrated Microscopy: Sept. 20-22, 1996






From: !Microscopy-request-at-Sparc5.Microscopy.Com (Sverker Enestrom)
Date: Tue Mar 19 09:13:29 +0100 1996
Subject: Re: Creutzfeld-Jakob disease

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Sender: kayton-at-ohsu.edu (Robert Kayton)
Message-Version: 2
} To: Microscopy-at-Sparc5.Microscopy.Com


} This information is at least 4 years old and may not be correct.
} About 4 years ago (maybe a little longer) there was a lengthy review
} article in either New England Journal of Medicine or JAMA regarding
} the slow human neurological disorders. At that time, there were at
} least 5 recognized diseases that were classified as "Alzheimer" or
} "Alzheimer-like". One of these was suspected to be of prion origin,
} although by now it may be classified as something else.
}
} One thing to consider if you are involved in studies involving human
} (or any mammalian) brain tissue is that in years past, prion diseases
} were commonly misdiagnosed as Alzheimers, and as humans (even
} pathologists) are not perfect, this still may be the case.

AD and prion diseases share the presence of amyloid precursor proteins
(beta-PP, APrP) but they are distinct diseases, both starting at the synapse
(beta-PP and PrP are proteins of the neuromuscular junction and CNS synapse).
In some of the prion diseases prion amyloid plaques are seen together with
paired helical filaments (NFT) making them "Alzheimer-like" as Budy writes.


*********************************************************
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
Fax: +46 13 13 22 57
*********************************************************






From: !Microscopy-request-at-Sparc5.Microscopy.Com (Robert Kayton,MAC,CROET)
Date: Mon Mar 18 16:40:07 EST 1996
Subject: Re: Creutzfeld-Jakob disease

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Sender: kayton-at-ohsu.edu (Robert Kayton)
Message-Version: 2
} To: Microscopy-at-Sparc5.Microscopy.Com

} As far as I know Alzheimers disease is not infectous---- Where did you get your
} information about Alzheimers disease?

This information is at least 4 years old and may not be correct.
About 4 years ago (maybe a little longer) there was a lengthy review
article in either New England Journal of Medicine or JAMA regarding
the slow human neurological disorders. At that time, there were at
least 5 recognized diseases that were classified as "Alzheimer" or
"Alzheimer-like". One of these was suspected to be of prion origin,
although by now it may be classified as something else.

One thing to consider if you are involved in studies involving human
(or any mammalian) brain tissue is that in years past, prion diseases
were commonly misdiagnosed as Alzheimers, and as humans (even
pathologists) are not perfect, this still may be the case.



W. L. Steffens, Ph.D
Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: !Microscopy-request-at-Sparc5.Microscopy.Com (Robert Kayton,MAC,CROET)
Date: Mon Mar 18 10:04:59 EST 1996
Subject: Re: Creutzfeld-Jakob disease

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Sender: kayton-at-ohsu.edu (Robert Kayton)
Message-Version: 2
} To: Microscopy-at-Sparc5.Microscopy.Com

} Has anyone heard of the slow viruses that can survive gluteraldehyde fixation?

The infectious agents that are able to survive fixation are the
prions. These agents contain protein only...no nucleic acid. Among
these are Creutzfeld-Jakob, scrapie, kuru,
Gersten-Straussler-Schenker, BSE (mad cow disease) and apparently
some forms of Alzheimers.

It is known that formaldehyde does not inactivate them, not surprising
since formaldehyde is one of the least cross-linking of all
fixatives, so far as proteins are concerned. My understanding is
that glut. does inactivate them, but don't take my word for it.

} On a similar note, there was an MSA-sponsored speaker a couple of years ago who
} was encouraging EM labs to make money by offering virus identification sevices
} to medical centers. The preparation protocol included advice to accept unfixed
} (ie. infectious), unidentified material and prepare negative stained samples on
} the bench in the laboratory.

We are a CLIA and State certified human clinical laboratory, licensed for
negative-stain virus identification. This has always been part of
our function as a veterinary EM lab, and for about 5 years we have
offered it as a service to the community. We do make a considerable
amount of money from it. We accept samples only from certain
reference laboratories and practitioners and these are only stool
samples, mostly (} 95%) from infantile diarrheas. Our submission form has an
entry for any unusual precautions that must be taken (ie HIV,
hepatitis, etc). Our understanding with our clients is that we don't
process such samples.
W. L. Steffens, Ph.D



Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Mon, 01 Apr 1996 17:09:46 -0600
Subject: Printers for digital images

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Fellow microscopists -

A few months ago there was a thread on the server dealing primarily with
flatbed scanners and the best technology for acquiring digital images. I may
have missed out on the part of the conversation dealing with printing
digital images, but perhaps one or some of you might summarize any thoughts
on what technologies were considered useful.

I run an EM lab in a Pathology department of a medical school. Most of my EM
is diagnostic work on kidney specimens and tumors. We printed out 4,500
images for each of the two past years. I would like to go digital, but
finding a printer that will handle this load cheaply and quickly, yet with
the required high quality is difficult. We plan to produce the usual 3 1/4"
X 4" negatives in our scope as we always have. Then we would scan the
negatives into a computer (IBM clone), storing them temporarily on a large
hard disk, and ultimately archiving them on a writable CD system. The prints
need not be durable as once they are studied and the case closed, they would
be trashed. The negatives would be archived as would be the image files on
the CD's.

The problem is that people are suggesting dye sub for quality and laser for
quick & dirty. I need QUICK & QUALITY......and cheap. Printing on a dye
sublimation printer would be too costly. There are some enhancement boards
that can be added to laser printers, and I have a friend who has printed
some of my scanned negatives on his Photoscan system, Philips's image
recording system. They come close to answering the problem, but I am
wondering what else there is out there. I believe that Philips's system may
be LaserPix or PhotoJet Plus from XLI Corp.

I realize that I may be asking you to repeat a conversation already carried
out, but if you could pass on the consensus opinions, I would appreciate it.


Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Mon, 01 Apr 1996 20:23:36 EST
Subject: Safety issues in EM lab

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

I know that my own safety committee gets under my own skin once in a
while.

But in over twenty six years of business, let me relate by far the most
serious injury that occurred in our firm. Considering that we work
around high voltage, use hazardous chemicals including osmium tetroxide,
deal with sometimes hazardous samples, what was our most serious
injury? Would you believe it involved Polaroid film?

One of the microscopists (who always claimed eye protection was not
needed in an SEM lab) was opening up a case of Type 52 film. The flaps
on the case, as it turned out were not creased, as they should have
been, therefore the tension present in the fold, when the sealing tape
on the top was slit with a razor, literally flew open, cutting the
corneal surface of one of the startled technician's eyes. Fortunately
sight was not lost in the eye, but there was a scar formed, and there
will be the need for lifetime care under a good ophthalmologist and who
knows what other impairments might show up with age.

Now in retrospect, nearly twenty years later, I still feel guilty about
not enforcing to an even greater degree, perhaps with the threat of
being dismissed, our own "rule" that safety glasses should be worn in
the laboratory. If an accident is going to happen, generally speaking
it will happen when it is least expected.

Now my point only is that some of these safety regulations, superfluous
as some of them might sound, often times do have some basis of logic
and rationale, my Polaroid film case being, I think, a good example.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: !Microscopy-request-at-Sparc5.Microscopy.Com :      ron-anderson-at-vnet.ibm.com (1-914-892-2225)
Date: Thu Mar 28 10:09:47 EST 96
Subject: A little humor

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Sender: kayton-at-ohsu.edu (Robert Kayton)
Message-Version: 2
} To: MICROSCOPY-at-Sparc5.Microscopy.Com

Sorry, I couldn't resist sharing this... It was sent to me 'as is'
with no author. I'm certainly not clever enough to write this.

Ron


I am the Very Model of a Modern Teenage Cyberpunk*


I am the very model of a modern teenage Cyberpunk
I rent my own apartment and it's full of electronic junk
I own a VAX, a 486, I've even got a PDP
I've finished Myst and Doom but I am stumped by Wing Commander III

I'm very well aquainted too with matters pornographical
I have a list of image sites, both overseas and national
So if you want to see a picture of that Anna Nichole Smith
I'll fire up my terminal and fetch for you a naughty GIF

I'm totally an anarchist, the government I'd like to wreck,
Though if they were to get blown up, who'd give to me my welfare cheque?
In short if you need answers that concern your electronic junk,
I am the very model of a modern teenage Cyberpunk

I know the ancient myths about RTM, Pengo and Mitnick
I 'hack' into computers and I then perform a credit check
I scare all my non-hacker friends with tales of cracker theivery
and even though I'm spouting crap they'll listen and believe in me

I've learned to spot a troll and I've seen flames about the way I spell,
I've traced badly forged cancels and seen napalm poured on AOL
I've laughed at all the newbies and their flailing cries of "You all Suck!"
I've been flamed by Carasso, with an anvil I have then been struck

I've hung around in alt.tasteless and seen war waged on rec.pets.cats
I've spent my time in talk.bizarre and used those stupid Relay Chats
In short, if you need answers that concern your electronic junk,
I am the very model of a modern teenage Cyberpunk

Well postings like "MAKE.MONEY.FAST", I am now somewhat wary at,
I have been "Global Killfiled" by the Joel Furr Commissariat,
When rosebud posts a lengthy rant 'bout Microsoft she swears is true,
I know that she is just another short lived kook without a clue

When I have learnt what progress has been made upon the Internet,
When I know something more than just a smattering of netiquette,
In short when I can have a world-wide soapbox on which I can stand
I've got no time for other things, like beer and trips to Disneyland

My life outside the Internet is very very sad you see
I cannot get my spots to fade, my social life's a tragedy,
But still if you need answers that concern your electronic junk,
I am the very model of a modern teenage Cyberpunk.

(With apologies to G&S)





From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Tue, 2 Apr 1996 09:24:20 +0100
Subject: Re: EM: Marine invertebrate texts

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} Dear Microscopists,
} Does anyone out there know of a textbook or any other reference material
} which covers integumental ultrastructure - cuticle, epithelium and
} associated structures and also oogenesis - in marine invertebrates. We have
} a marine science student who is studying subclass Ascothoracida who is
} having problems getting the ultrastructure info she needs.

You might browse the "Microscopic Anatomy of Invertebrates" series
(Frederick W. Harrison, Burton J. Bogitsh, Eds.), Wiley-Liss, New York,
1991. It=B4s a highly comprehensive series of 15 volumes, covering in-depth
ultrastructure from Protozoa up to Hemichordata, Chaetognatha, and else.

-Dietmar-


+++ Dietmar Reiter {dietmar.reiter-at-uibk.ac.at} ++++++++++++++++
+++ Dept. of Zoology and Limnology, University of Innsbruck ++++
+++ Technikerstrasse 25, A - 6020 Innsbruck, Austria +++++++++
+++ ph: (+43)-512-507-6170 (-6161), fax: ..-507-2930 (-2957) +++






From: M.F. Butler :      mfb12-at-cus.cam.ac.uk
Date: Tue, 2 Apr 1996 13:24:48 +0100 (BST)
Subject: unsubscribe

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From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Tue, 2 Apr 1996 07:57:15 -0500
Subject: RE: MSDS

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Message-ID: {199604021254.HAA27062-at-IndyNet.indy.net}
To: "Joiner Cartwright, Jr., Ph.D." {joiner-at-bcm.tmc.edu} ,
Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

DOT regulations DO NOT require that an MSDS accompany every shipment. DOT
does have strict requirements for packaging and labeling (exterior) that
are specific to the contents. DOT also requires that shippers of hazardous
material have a 24 hour telephone number (their own, or a service) that can
answer questions about the material. Both DOT and OSHA require training of
personel that handle hazardous materials.

David Rothbard

--
Institute of Paper Science and Technology






From: James R. Stets :      stetsjr-at-ttown.apci.com
Date: Tue, 2 Apr 1996 07:52:17 -0500 (EST)
Subject: Re: Kevex 8000 Data to PC

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Michael Lamvik asked about moving data from a Kevex 8000 to a PC. Kevex
used to sell a package to do this that cost about $1000. This bought you
a cable that connected to one of your printer ports on the 8000 and your
PC, a diskette with Kermit, software for the 8000, and a very thick manual.

I used it many times to move images from our 8000 to a Macintosh, and it
worked fine although it's not fast.

I don't know if Kevex sells this anymore since the 8000 is pretty old. A
gentleman named Robert Schaller was very helpful in getting us set up
with it, but this was at least 5 years ago and he may or may not still be
with Kevex.

Jim Stets
Air Products and Chemicals, Inc.
Allentown, PA
stetsjr-at-ttown.apci.com
My opinions are my own, not my employer's.




From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Tue, 2 Apr 1996 11:23:58 -0500
Subject: Re: HRP substrates

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Message-ID: {F54A613101F70300-at-mhs.unc.edu}
In-Reply-To: {F422613101F70300}

We have been using the EnVision kit sold by DAKO with great success.
This kit does come with a "stable" DAB solution which works very well.
Under some conditions when we need to increase the intensity we use the
"Quick DAB Enhancer Solution" sold by Innovex Biosciences which works
very well and will not change the color of the DAB like nickle chloride.

The usual disclaimer applies to both of the companies mentioned above ie:
I'm simply a satisfied customer and recieve no renueration for naming
their products

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: Joe D Geller :      geller-at-world.std.com
Date: Tue, 2 Apr 1996 08:52:31 -0500 (EST)
Subject: Re: SEM Image distortion

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If you have a digital imaging system it is possible to reduce the number
of sources of trouble. Does the distortion show up on the digitally
recorded image?

Joe Geller
Geller Microanalytical Lab
426e Boston St.
Topsfield, MA 01983-1212
508 887-7000

On Mon, 1 Apr 1996, Neuberger, Damian wrote:

}
} Hello All:
}
} We have a JEOL JSM-6300F microscope that exhibits a not-so-slight Y-image
} distortion. I hope that I can explain it clearly and that someone out there
} may have suggestion(s) as to the source of the problem that we have not been
} able to get resolved by field service personnel.
}
} Materials: (1) SEM; 2) NIST SRM 484f SEM magnification standard; 3) 2000
} copper mesh grid (Pella Cat #631C) that has been calibrated as a secondary
} standard with the NIST standard; 4) Polaroid Type 53 film, 4x5".
}
} Conditions: Image of the mesh grid recorded at X2000, 5 keV, 25 mm WD,
} aperture #4 (30 micrometers), probe current setting #8 (1x10-11), camera
} aperture setting 5.6, scan rate 80 (sec/scan?). Micrograph is oriented so
} that the 5" dimension is along the X-axis. Scan direction top to bottom
} (Y-axis). Scan rotation is OFF, tilt correction is OFF, specimen tilt is 0,
} SEI mode.
}
} Problem: The micrograph as well as the viewing monitors exhibit a
} distortion in the Y?-axis. That is, the vertical spacing between grid bars
} on one end of the X-axis of the photo is about 1.5 to 2 mm greater (out of
} 74mm) at the right end than on the left end. This distortion is easily seen
} in the image of the grid. (Wouldn't a photo in this email make things a
} whole lot easier!)
}
} Solutions: That's where you knowledgeable microscopists come in. Any ideas
} will be gratefully accepted and tested!
}
} Thanks so much, everyone.
}
} Damian Neuberger
} neuberd-at-baxter.com
}
}
}




From: Walt Bobrowski (313) 996-7814 :      bobroww-at-aa.wl.com
Date: Tue, 02 Apr 1996 08:11:53 -0400 (EDT)
Subject: Re: Printers for digital images

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Check out DOUBLE RES IV from Laser Printer Accessories Corp., a board you
install into your current laserjet II or III to get 600 dpi quality. We have
one and it really does make a BIG difference. 1-800-225-4098.

Walter F. Bobrowski
Subcellular Pathology
Parke-Davis Pharmaceutical Research
Ann Arbor, MI 48105

TEL: 313-996-7814
FAX: 313-996-5001
E-Mail: BOBROWW-at-AA.WL.COM


} Fellow microscopists -
}
} A few months ago there was a thread on the server dealing primarily with
} flatbed scanners and the best technology for acquiring digital images. I may
} have missed out on the part of the conversation dealing with printing
} digital images, but perhaps one or some of you might summarize any thoughts
} on what technologies were considered useful.
}
} I run an EM lab in a Pathology department of a medical school. Most of my EM
} is diagnostic work on kidney specimens and tumors. We printed out 4,500
} images for each of the two past years. I would like to go digital, but
} finding a printer that will handle this load cheaply and quickly, yet with
} the required high quality is difficult. We plan to produce the usual 3 1/4"
} X 4" negatives in our scope as we always have. Then we would scan the
} negatives into a computer (IBM clone), storing them temporarily on a large
} hard disk, and ultimately archiving them on a writable CD system. The prints
} need not be durable as once they are studied and the case closed, they would
} be trashed. The negatives would be archived as would be the image files on
} the CD's.
}
} The problem is that people are suggesting dye sub for quality and laser for
} quick & dirty. I need QUICK & QUALITY......and cheap. Printing on a dye
} sublimation printer would be too costly. There are some enhancement boards
} that can be added to laser printers, and I have a friend who has printed
} some of my scanned negatives on his Photoscan system, Philips's image
} recording system. They come close to answering the problem, but I am
} wondering what else there is out there. I believe that Philips's system may
} be LaserPix or PhotoJet Plus from XLI Corp.
}
} I realize that I may be asking you to repeat a conversation already carried
} out, but if you could pass on the consensus opinions, I would appreciate it.
}
}
} Joiner Cartwright, Jr., Ph.D.
}
} Director, Electron Microscopy tel.: (713)798-4658
} Department of Pathology, Rm.286-A FAX: (713)798-3945
} Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
} One Baylor Plaza Compuserve: 71555,1206
} Houston, Texas 77030 U.S.A.
}






From: James Patrick :      JPATRICK-at-OPUS.MCO.EDU
Date: Tue, 02 Apr 1996 13:50:44 -0500 (EST)
Subject: unsubscribe

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From: James Patrick :      JPATRICK-at-OPUS.MCO.EDU
Date: Tue, 02 Apr 1996 13:50:44 -0500 (EST)
Subject: unsubscribe

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From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Tue, 02 Apr 1996 12:46:48 -0600
Subject: Re: Printers for digital images

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Message-Id: {199604021848.MAA01689-at-watson.bcm.tmc.edu}
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At 08:11 AM 4/2/96 -0400, you wrote:

} Check out DOUBLE RES IV from Laser Printer Accessories Corp., a board you
} install into your current laserjet II or III to get 600 dpi quality. We have
} one and it really does make a BIG difference. 1-800-225-4098.
}
} Walter F. Bobrowski
} Subcellular Pathology
} Parke-Davis Pharmaceutical Research
} Ann Arbor, MI 48105
}
} TEL: 313-996-7814
} FAX: 313-996-5001
} E-Mail: BOBROWW-at-AA.WL.COM

****************************
Walter -

Is the 600 dpi output of this system better than the 600 dpi output of the
LaserJet 4 that we already have? There are boards that hot-rod the LaserJet
4 up to "2,400 equivalent dpi". One wonders if they will show "equivalent"
deposits in my kidney specimens. Thank you for your reply.


* * Joiner Cartwright, Jr. * *





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Tue, 02 Apr 1996 12:46:38 -0600
Subject: Re: Printers for digital images

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Scott -
Here is a reply that may interest you.
Joiner


At 09:35 AM 4/2/96 -0500, you wrote:
} In response to your recent message posted on the newsgroup concerning
} digital printers, there are several options. The first going to laser
} printers, and the second being dye sub printers. The cost of laser is
} obviously cheap, but not high enough quality perhaps for publication or
} reports where a photo is required. Dye sub printers ex: sony makes two
} digital dye sub printers. The first being model UP-D1500CN, it ouputs a
} picture size of approx. 3 5/8" x 4 3/4" which is near photographic quality.
} This printer sells for approx. $ 1,850.00 US and the print cost is approx.
} .86 cents. for color images. The other printer from sony is the UPD-8800
} with Interface card is approx. $7,300 US and the print cost for 8.5" x 11"
} is approx. $ 2.20 These printers will ouput a print in approx. 60 seconds.
} I do have sample prints if you require to see some.
}
} Sony makes a digital thermal printer that outputs approx. a polaroid size
} image on thermal paper in about 3.5 seconds, cost per print is approx. 9 cents.
}
} Epson makes some low cost printers, with a decent low cost image, the only
} problem with laser or ink jet with these images is that it takes quite a
} while to print.
}
} I don't know if I was helpful or not, but if I can be of any assistance,
} please feel free to contact me at your convenience.
}
}
} Best Regards,
}
} Philip Slakmon
} Scott Scientific
}
}
} _______________________________________________
} SCOTT SCIENTIFIC
} P.O. Box 66552 Station Cavendish,
} Montreal, Quebec, H4W 3J6, Canada
}
} Telephone: 514-485-2309 Fax: 514-485-9931
} Voice Mail: 514-888-6509
}
} WWW Site: http://www.ScottScientific.com
}
} E-Mail: slakmon-at-scottscientific.com
} info-at-scottscientific.com
} sales-at-scottscientific.com
} admin-at-scottscientific.com
} _________________________________________________
}
}
**********************************
Philip -

Thank you for your reply. Dyesub, as you see, may be capable of turning out
images of good enough quality to see, for example, the subtle
ultrastructural changes in kidney disease, but not at a price that we can
afford. And laser, although fast and cheap, cannot produce the image quality
necessary to show these subtle changes. One or more of the laser enhancement
boards MAY be the answer, but I am not yet convinced.


* * Joiner Cartwright, Jr. * *





From: Len Adleman :      adleman-at-pollux.usc.edu
Date: Tue, 2 Apr 1996 14:24:45 -0800 (PST)
Subject: Re: unsubscribe

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From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Tue, 02 Apr 1996 17:20:08 -0600
Subject: Re: Printers for digital images

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At 01:51 PM 4/2/96 EST, you wrote:

} I run a similar facility in the Pathology Department of a Veterinary
} College, but we also serve the entire College as well as some other
} parts of the university.
} With our SEM, we are entirely digital now...

*****************
Dr. Steffens -

Thank you for your reply. The savings are one of the main reasons that I
want to go digital. That includes savings in time as well as money. I am
considering the LaserPix. However there are some other technologies out
there that I want to look at as well.


* * Joiner Cartwright, Jr. * *





From: Jeff Allbright :      jeffallb-at-gol.com
Date: Wed, 3 Apr 1996 07:16:02 +-900
Subject: Kevex 8000 Data to PC

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Message-ID: {01BB212D.85B076C0-at-ppp236.gol.com}

Kevex still offers the 8000 to PC communication package using Kermit.
There is also a high speed data transfer option available using a SCSI
interface card. This is especially useful for transferring large image
files, etc. More information is available from Kevex Customer Service at
+1 (415) 591-3600.

- Jeff Allbright, Tokyo



Michael Lamvik asked about moving data from a Kevex 8000 to a PC. Kevex
used to sell a package to do this that cost about $1000. This bought you
a cable that connected to one of your printer ports on the 8000 and your
PC, a diskette with Kermit, software for the 8000, and a very thick manual.

I used it many times to move images from our 8000 to a Macintosh, and it
worked fine although it's not fast.

I don't know if Kevex sells this anymore since the 8000 is pretty old. A
gentleman named Robert Schaller was very helpful in getting us set up
with it, but this was at least 5 years ago and he may or may not still be
with Kevex.

Jim Stets
Air Products and Chemicals, Inc.
Allentown, PA
stetsjr-at-ttown.apci.com
My opinions are my own, not my employer's.








From: Jeff Allbright :      jeffallb-at-gol.com
Date: Wed, 3 Apr 1996 07:20:33 +-900
Subject: Kevex 8000 Data to PC

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Message-ID: {01BB212E.209B9520-at-ppp239.gol.com}


Kevex still offers the 8000 to PC communication package using Kermit.
There is also a high speed data transfer option available using a SCSI
interface card. This is especially useful for transferring large image
files, etc. More information is available from Kevex Customer Service at
+1 (415) 591-3600.

- Jeff Allbright, Tokyo



Michael Lamvik asked about moving data from a Kevex 8000 to a PC. Kevex
used to sell a package to do this that cost about $1000. This bought you
a cable that connected to one of your printer ports on the 8000 and your
PC, a diskette with Kermit, software for the 8000, and a very thick manual.

I used it many times to move images from our 8000 to a Macintosh, and it
worked fine although it's not fast.

I don't know if Kevex sells this anymore since the 8000 is pretty old. A
gentleman named Robert Schaller was very helpful in getting us set up
with it, but this was at least 5 years ago and he may or may not still be
with Kevex.

Jim Stets
Air Products and Chemicals, Inc.
Allentown, PA
stetsjr-at-ttown.apci.com
My opinions are my own, not my employer's.









From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 2 Apr 1996 13:51:24 EST
Subject: Re: Printers for digital images

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microscopy-at-Sparc5.Microscopy.Com

} I run an EM lab in a Pathology department of a medical school. Most of my EM
} is diagnostic work on kidney specimens and tumors. We printed out 4,500
} images for each of the two past years. I would like to go digital, but
} finding a printer that will handle this load cheaply and quickly, yet with
} the required high quality is difficult. We plan to produce the usual 3 1/4"
} X 4" negatives in our scope as we always have. Then we would scan the
} negatives into a computer (IBM clone), storing them temporarily on a large
} hard disk, and ultimately archiving them on a writable CD system. The prints
} need not be durable as once they are studied and the case closed, they would
} be trashed. The negatives would be archived as would be the image files on
} the CD's.
}
} The problem is that people are suggesting dye sub for quality and laser for
} quick & dirty. I need QUICK & QUALITY......and cheap. Printing on a dye
} sublimation printer would be too costly. There are some enhancement boards
} that can be added to laser printers, and I have a friend who has printed
} some of my scanned negatives on his Photoscan system, Philips's image
} recording system. They come close to answering the problem, but I am
} wondering what else there is out there. I believe that Philips's system may
} be LaserPix or PhotoJet Plus from XLI Corp.
}
I run a similar facility in the Pathology Department of a Veterinary
College, but we also serve the entire College as well as some other
parts of the university.
With our SEM, we are entirely digital now...I don't even support film
on it. With the TEM, we have a side-mounted digital camera system
(JEOL TV-CAM) that is used for image acquisition, but we still use film for publication
purposes. Most of the TEM images are for study purposes only, and
for this we dump the image (using the system's software) to a
Laserjet 4 printer through a Laserpix board. After a bit of
processing, ie BCG adjustment, unsharp mask sharpening, etc., you get
a "work print" that is usually acceptable. For higher quality, we
also have a Codonics dye sublimation printer, on which you can put 4
images per page. For TEM, its certainly better quality than the
Laserjet, but is usually not publication quality unless you do some
intensive processing. SEM and light micrographs on it however are
certainly publication quality.
Since most images we take will not be published, we get along fine
with the Laserjet prints for TEM. We do use a high quality glossy
paper in it which drives up the cost of the prints to about .12 each.
That still beats the 2.00+ of the Codonics.
Since going mostly digital, our TEM film images have dropped from
about 2000 per year to about 200 per year. The savings in tech
labor, direct costs, and chemical wastes are quite substantial.




W. L. Steffens, Ph.D
Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Tue, 2 Apr 1996 19:18:16 -0600
Subject: Re: EM: Marine invertebrate texts

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} } Dear Microscopists,
} } Does anyone out there know of a textbook or any other reference material
} } which covers integumental ultrastructure - cuticle, epithelium and
} } associated structures and also oogenesis - in marine invertebrates. We hav=
e
} } a marine science student who is studying subclass Ascothoracida who is
} } having problems getting the ultrastructure info she needs.
}
} You might browse the "Microscopic Anatomy of Invertebrates" series
} (Frederick W. Harrison, Burton J. Bogitsh, Eds.), Wiley-Liss, New York,
} 1991. It=B4s a highly comprehensive series of 15 volumes, covering in-depth
} ultrastructure from Protozoa up to Hemichordata, Chaetognatha, and else.
}
} -Dietmar-

As well as this ref, there is:

Neville, A.C. 1975. _The Biology of the Arthropod Cuticle_. Vol,
4/5 in the series "Zoophysiology and Ecology". Springer-Verlag.

Nothing on Ascothoracida in specific, but a good general ref, and
literature review up to about 1974.
Phil

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Tue, 02 Apr 1996 17:20:17 -0600
Subject: Printers for digital images

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Message-Id: {199604022321.RAA06384-at-watson.bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I recently posted a query concerning printers suitable for producing high
quality monochrome images from micrographs scanned into a computer....a
printer that would allow me to "go entirely digital" and get me out of the
darkroom, at least for all imaging except the most demanding applications. I
have received a number of replies. Boiled down, the answers include:

1.) For quick & dirty, but usable work prints, a laser printer producing
600+ dpi;

2.) For high quality, a dye sublimation printer; and

3.) An intermediate would be a laser printer with one of the after market
enhancement boards that runs the lasers resolution up to 1,200 dpi or "2,400
dpi equivalent", whatever that means;

4.) A fourth suggestion two or three people made was the Harris PhotoPro
2000 Gray Scale Digital Printer. This is a $10,000.00 silver salt laser
printer that claims only 256 dpi, but each dot has 256 gray levels
available. It was stated that the cost/print was less than $1.00.

Do any of you have any experience with this latter machine, and would you be
willing to share your thoughts? Does the increased contrast resolution make
up for the POSSIBLY low spatial resolution? Do I really want to spend ten
big ones on a black & white printer?




Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: stpiera-at-lepton.hmco.com (Aaron Stpierre)
Date: Tue, 2 Apr 1996 22:48:15 -0500 (EST)
Subject: unsub

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From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Tue, 2 Apr 1996 20:53:19 -0500
Subject: Re: Printers for digital images: Tests

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Message-Id: {v01520d01ad87835f46cf-at-[155.37.2.10]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

In response to last years questions raised on the performance of digital
printers I tested several printers with a 1Kx1K image. Printer performances
were compared at the level of whole page images and 10x enlargements of the
printed images (LaserJet, Lazar Print, Dye sub,). Results are available at

http://panda.uchc.edu/htklaus/DigiLab/Printing.html

In conclusion, you have to ask how many lines per inch are printed and at
how many gray levels (not always the same as dots per inch), plain paper or
special paper, permanence of the print, printing time, printing costs. I
print 300 lpi at 256 gray levels (4,800 dpi) on plain paper in 20-45 sec
for 1-10 MB of images per page at 3 cents per page with a HP LaserJet and
enhancer board in archival quality.

Best regards Klaus


*****************************************

} At 08:11 AM 4/2/96 -0400, you wrote:
}
} } Check out DOUBLE RES IV from Laser Printer Accessories Corp., a board you
} } install into your current laserjet II or III to get 600 dpi quality. We have
} } one and it really does make a BIG difference. 1-800-225-4098.
} }
} } Walter F. Bobrowski
} } Subcellular Pathology
} } Parke-Davis Pharmaceutical Research
} } Ann Arbor, MI 48105
} }
} } TEL: 313-996-7814
} } FAX: 313-996-5001
} } E-Mail: BOBROWW-at-AA.WL.COM
}
} ****************************
} Walter -
}
} Is the 600 dpi output of this system better than the 600 dpi output of the
} LaserJet 4 that we already have? There are boards that hot-rod the LaserJet
} 4 up to "2,400 equivalent dpi". One wonders if they will show "equivalent"
} deposits in my kidney specimens. Thank you for your reply.
}
}
} * * Joiner Cartwright, Jr. * *

******************************************************************************
* : *
* Klaus-Ruediger Peters, Ph.D. : WWW Home Page: *
* Director, Molecular Imaging Laboratgory : *
* Biomolecular Structure Analysis Center : Molecular Imaging Laboratory *
* University of Connecticut Health Center : http://panda.uchc.edu/ *
* 263 Farmington Ave. : htklaus/index.html *
* Farmington, CT 06030-2017; U.S.A : Differential Hysteresis *
* : Processing Demo at http:// *
* Tel: (203) 679-3977; Fax: (203) 679-1989 : panda.uchc.edu./htbit/indiv/ *
* e-mail: Peters-at-BSAC.UCHC.EDU : /software_docs/dhp.html *
* : *
****************************************************************************
**






From: ota-at-sun3.oulu.fi (Olli Taikina-aho)
Date: Wed, 3 Apr 1996 09:42:54 +0300 (EET DST)
Subject: point counter needed

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Hello friends

Could someone tell me if there is any company delivering mechanical point
counter systems. I need it for a polarizing microscope for geological samples.
Olli Taikina-aho
University of Oulu
Institute of Electron Optics
Box 400
SF-90571 Oulu
ota-at-cc.oulu.fi
tel. +358-81-5533142
fax. +358-81-5533149





From: houpt-at-worldaccess.nl (houpt)
Date: Wed, 3 Apr 1996 12:24:27 +0100
Subject: unsubscribe

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BIOMET
P.M.Houpt
The Hague.
The Netherlands.






From: French Michele H :      French_Michele_H.PRILVMS3-at-msmail.bms.com
Date: Wed, 03 Apr 1996 09:16:24 +0800 (U)
Subject: subscribe

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From: Jose'Angel Conchello :      josec-at-abbe.wustl.edu
Date: Wed, 3 Apr 1996 10:41:40 -0600
Subject: BiOS '97 Call For Papers

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Three-Dimensional Microscopy: Imaging Acquisition and Processing IV

Conference Chairs: Carol J. Cogswell, Univ. of Sydney (Australia); Jose-Angel
Conchello, Washington Univ.; Tony Wilson, Univ. of Oxford (UK)

Program Committee: Alan Bearden, Univ. of California/Berkeley; G. J.
Brakenhoff, Univ. of Amsterdam (Netherlands); Kjell Carlsson, Royal Institute
of Technology (Sweden); ; Seth Goldstein, National Institutes of Health;
Gordon S. Kino, Stanford Univ.; Andres Kriete, Univ. Giessen (FRG); Frederick
Lanni, Carnegie Mellon Univ.

This conference will explore the rapidly developing field of three-dimensional
microscopy. Consideration will be given to the characteristics of the overall
system design, as well as to the topics of image formation, image recording,
deconvolution in 2,3 or more dimensions, and digital methods of producing and
displaying the resulting 3D reconstruction. Recent innovations in 3D
microscopy are having a large impact in the biological and medical fields, as
well as in materials science and the semiconductor industry. Many microscopes
are now fully integrated systems, including computer hardware and software. It
is hoped that the broad range of relevant topics being presented at this
symposium will serve to encourage interaction among instrumentation engineers,
computer image analysts, and researchers in the various fields of application.

Papers are invited in the following and related areas:
- confocal microscopy
- 3D image formation
- instrumentation for 3D microscopy
- time-resolved image acquisition systems
- image processing and analysis
- 3D image reconstruction
- deconvolution in 2/3 or more dimensions
- super-resolution
- spatiotemporal reconstruction of living cells and tissues
- applications of 3D microscopy in materials science
- profilometry
- image visualization techniques for 3D microscopy systems, including volume
rendering, animation, stereoscopic and holographic displays



This conference is just one of 27 topics to be held at the BiOS '97 symposium,
which is part of the larger event called Photonics West '97:
8-14 February 1997
San Jose Convention Center
San Jose, California USA

TO OBTAIN ALL CALLS FOR PAPERS ELECTRONICALLY
The calls for papers for all conferences in the BiOS '97 symposium (as well as
all other symposia that are part of Photonics West) will be available
1 May on the SPIE Web site
http://www.spie.org/web/meetings/calls/submissions.html;
by anonymous FTP
ftp://spie.org/meetings/calls/PW97_readme.txt;
or by e-mail file retrieval
send a message to info-spie-request-at-spie.org with the following in the message
body:
send [meetings.calls]PW97_readme.txt

For a printed BiOS '97 call for papers or other information:
E-mail: PW97-at-spie.org
Fax: 360/647-1445
Phone: 360/676-3290

IMPORTANT DEADLINES
Paper Abstracts Due from Authors:
15 July 1996 (post-meeting proceedings)
Advance Programs due from Chairs:
12 August 1996 (post-meeting proceedings)
Camera-Ready Abstracts due from Authors:
9 December 1996
Manuscripts Due from Authors:
13 January 1997 (post-meeting proceedings)

GUIDELINES FOR SUBMITTING AN ABSTRACT

Send a 250 word abstract of your paper, by 15 July 1996, in ONE of the
following ways:

* SPIE WEB - Complete the convenient form found at SPIE Web site:
http://www.spie.org/web/meetings/calls/submissions.html

* or E-MAIL each abstract separately to: abstracts-at-spie.org in ASCII text
(not encoded) format. To ensure receipt and proper processing of your
abstract, write on SUBJECT line: PW '97 and Conference Chair.

Example: SUBJECT: PW97 (John Smith)

* or MAIL three copies of your abstract to:
BiOS '97
SPIE, P.O. Box 10, Bellingham, WA 98227-0010 USA
Shipping address: 1000 20th St., Bellingham, WA 98225 USA
Telephone 360/676-3290

* or FAX one copy to SPIE at 360/647-1445 (send each abstract separately).


Be sure each abstract includes the following:

1. CONFERENCE CHAIR and CONFERENCE TITLE (submit to ONLY ONE conference)
to which the abstract is submitted

2. AUTHOR LISTING (List principal author first)
for each author: full name and affiliation, mailing address, phone/fax
numbers, email

3. ABSTRACT/PAPER TITLE

4. ABSTRACT TEXT: 250 words

5. KEYWORDS: maximum of 5 keywords

6. BRIEF BIOGRAPHY of the principal author: 50-100 words

Please contact SPIE if you have any questions or require further information.


Marilyn E. Gorsuch, Technical Programs Manager
SPIE
PO Box 10
1000-20th Street
Bellingham, WA 98225
Ph: 360/676-3290
Fax: 360/647-1445
e-mail: marilyn-at-spie.org





From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Wed, 3 Apr 1996 12:47:28 -0500
Subject: Sprinklers in the EM Lab

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Message-Id: {v0213050bad8867979a4c-at-[199.77.235.102]}
Mime-Version: 1.0
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Like many of you , I have worked in EM laboratories with water (fire
activiated) sprinklers overhead. In some cases I have successfully lobbied
to have them removed, as I felt there was a greater danger of electrical
shock or massive instrument damage if they tripped in error (beware the
pipefitter). However, there is always the possibility that your EM could
be the source of a fire that causes great damage and injury. How do people
feel about this?

David Rothbard

--
Institute of Paper Science and Technology






From: samso-at-orkney.ph.albany.edu
Date: Wed, 03 Apr 1996 13:28:24 EST
Subject: unsubscribe

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From: John M. Libert :      jlibert-at-cpcug.org
Date: Wed, 03 Apr 1996 11:53:52 -0800
Subject: Re: point counter needed

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Message-ID: {3162D750.4D11-at-cpcug.org}

Olli Taikina-aho wrote:
}
} Hello friends
}
} Could someone tell me if there is any company delivering mechanical point
} counter systems. I need it for a polarizing microscope for geological samples.
} Olli Taikina-aho
} University of Oulu
} Institute of Electron Optics
} Box 400
} SF-90571 Oulu
} ota-at-cc.oulu.fi
} tel. +358-81-5533142
} fax. +358-81-5533149Yes. Prior Scientific, Inc of Rockland, MA sells an electro-mechanical
pointcounter. It has a number of nice features and is fairly simple. Give
them a call at 617-878-8442 (FAX 617-878-8736).

John M. Libert
OPELCO
OPtical ELements COrporation
Sterling, VA.
703-471-0080 Ext. 217




From: Leslie Gartner :      lgartner-at-umabnet.ab.umd.edu
Date: Wed, 3 Apr 1996 15:24:03 -0500 (EST)
Subject: HELP Unsubscribe

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I apologize for posting this to the entire community, but I no longer
have the majordomo address to unsubscribe nor the proper procedure.
Please e-mail me directly to reduce bandwidth clutter.

Thank you,

Les Gartner
lgartner-at-umabnet.ab.umd.edu







From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Wed, 3 Apr 1996 16:00:50 -0500
Subject: Re: Sprinklers in the EM Lab

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Message-Id: {v0213050cad8896f9b99f-at-[199.77.235.102]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Several people have mentioned Halon to me, but I was under the impression
that Halon has fallen out of favor due to potential toxicity and
suffocation.

David Rothbard

--
Institute of Paper Science and Technology






From: rnessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Wed, 3 Apr 1996 15:51:49 -0600 (CST)
Subject: Re: Sprinklers in the EM Lab

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On Wed, 3 Apr 1996, David Rothbard wrote:

} Like many of you , I have worked in EM laboratories with water (fire
} activiated) sprinklers overhead. In some cases I have successfully lobbied
} to have them removed, as I felt there was a greater danger of electrical
} shock or massive instrument damage if they tripped in error (beware the
} pipefitter). However, there is always the possibility that your EM could
} be the source of a fire that causes great damage and injury. How do people
} feel about this?
}
} David Rothbard
}
} --
} Institute of Paper Science and Technology
}
Our lab is equipped with a Halon fire suppression system. I can't
verify that it works, as we have never needed it :)


Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu





From: Joyce Craig :      bafpjec-at-uxa.ecn.bgu.edu
Date: Wed, 3 Apr 1996 12:04:28 -0600 (CST)
Subject: LR White

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We are using LR white for the first time in anticipation of doing some
immunocytochemistry and also because I have several people who are
working with bacteria and fungi.
We have been fixing the bacteria in 2 + 2, trying different
concentrations of buffer because the bacteria grows in a 25% sodium
chloride. Then we mix the bacteria with agar, fix again, osmicate, and
dehydrate to 70%. Then we mix the LR White with 70 % for infiltration, then
straight LR White finally flat embed in LR White and polymerize at 60
degreesC.
We wrap the embedding molds in Saran wrap (not an off-brand) before
polymerization.
The blocks in blue JB4 molds hardened, but have a surface stickiness.
The blocks in the clear molds disappeared. The black blocks of osmicated
agar are there but the epoxy is gone.
Does anyone have any thoughts on this?
I really like running up nice blocks of pancreas and kidney and even
cardiac muscle in epoxy and taking pretty pictures in the microscope but
unfortunately no-one here is doing this nice rewarding basic work.




From: vit-at-felix.scvnet.com (Jim Yankovich)
Date: Wed, 3 Apr 1996 17:31:37 -0800
Subject: Unsubscribe

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From: rgwhite-at-vaxc.cc.monash.edu.au (Rosemary White)
Date: Thu, 04 Apr 1996 09:16:15 +1200
Subject: Postdoc - TEM, fluorescence (maybe confocal)

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POSTDOCTORAL FELLOWSHIP IN PLANT CELL BIOLOGY

Applications are invited for a postdoctoral fellowship on a 3-year project
investigating computer modelling of macromolecular structures in plant
cells. Part of this work requires preparation of TEM and
immunofluorescence micrographs for comparison with computer generated
images of plant cytoskeleton and plasmodesmata. We are also investigating
various aspects of the interactions between these cell components and their
dynamic behaviour in cells. Some confocal fluorescence microscopy may be
required.

Applicants should have experience in TEM and immunofluorescence of plant
material. Experience in freeze-substitution techniques and
immunofluorescence of the plant cytoskeleton would be an advantage.

The position is available from 1 July, and the successful candidate will be
expected to take up the appointment by 1 September 1996 at the latest.
Salary will commence at $(Australian)37,345. The appointment will be for
12 months in the first instance, renewable for a further 12 months (longer
if new grant application is successful). Some remuneration for removal
expenses will be available.

Applications should arrive by 4 May 1996, with CV naming 2 referees.

For further information, contact


Rosemary White
__ /
Department of Ecology _/ \__/ \
and Evolutionary Biology / \
Monash University / Australia \
Clayton, Victoria 3168 \ ____ /
phone 61-3-9905 5670 \_/ \_*_/
fax 61-3-9905 5613 __
email rgwhite-at-vaxc.cc.monash.edu.au \/






From: Sarka Lhotak :      lhotaks-at-fhs.csu.mcmaster.ca
Date: Wed, 3 Apr 1996 21:21:55 -0500 (EST)
Subject: immuno:source of antibodies

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Dear immunobuffs,

I was very impressed with the ChemExpo www site that
was recommended here recently. I wonder whether there is
something similar from antibody producers that would enable to
locate a source of a particular antibody easily. My search for
this was unsuccessful. Thanks for your help.

Sarka Lhotak
EM Facility, McMaster University
Hamilton, Ontario, Canada
lhotaks-at-fhs.mcmaster.ca




From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 4/3/96 3:48 PM
Subject: Sprinklers in the EM Lab

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Like many of you , I have worked in EM laboratories with water (fire
activiated) sprinklers overhead. In some cases I have successfully lobbied
to have them removed, as I felt there was a greater danger of electrical
shock or massive instrument damage if they tripped in error (beware the
pipefitter). However, there is always the possibility that your EM could be
the source of a fire that causes great damage and injury. How do people
feel about this?

David Rothbard

--
Institute of Paper Science and Technology







From: Microscopy-request
Date: Wednesday, April 03, 1996 5:16PM
Subject: Re: Sprinklers in the EM Lab

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David;

A few years ago, we had a very bad rainstorm in Southern California. The
roof
of our R&D building (which houses the SEM) leaked, and the ceiling tile got
so
soaked that it fell in on the SEM console. When I came in the next morning,

there was a steady stream of water pouring into the keyboard and down into
the
electronics of the SEM. Obviously, the SEM had tripped off. I unplugged it
and
called the vendor (Cambridge, at the time), since we were under a service
contract. End result - we allowed it to dry for 3 days and fired it up. It

worked fine, and has been operational ever since; no problems have been
attributed to this incident. In summary, I believe that the risks
associated
with fire far outweigh the risks associated with water.

Regards,

Bob




From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Thu, 4 Apr 1996 8:07:25 -0500
Subject: Re: immuno:source of antibodies

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Message-ID: {85C4633101F70300-at-mhs.unc.edu}
In-Reply-To: {7DC4633101F70300}

I haven't found anything on the net but an excellent (and not to
expensive ) refferance is "Linscotts Directory of Immunological and
Biological Reagents" the send out constant updates and is available in
written and PC compatible form. The phone # for additional information
is 707-544-9555.

regards, bob

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Thu, 4 Apr 1996 08:20:06 GMT
Subject: Re: LR White

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I maintain an archive of most of the biological EM related
material that comes across this list. Since you have net acess I will assume
you can get acess to the www. Go to the web page listed at the bottom of
this message and click of the "Tips & Tricks Wizard" In there is a file
called "Embedding with LR White" which may be of use to you. If for some
reason you do not have acess to the web or cannot read the file, let me know
and I will e-mail it to you .



At 12:04 PM 4/3/96 -0600, you wrote:
} We are using LR white for the first time in anticipation of doing some
} immunocytochemistry and also because I have several people who are
} working with bacteria and fungi.
} We have been fixing the bacteria in 2 + 2, trying different
} concentrations of buffer because the bacteria grows in a 25% sodium
} chloride. Then we mix the bacteria with agar, fix again, osmicate, and
} dehydrate to 70%. Then we mix the LR White with 70 % for infiltration, then
} straight LR White finally flat embed in LR White and polymerize at 60
} degreesC.
} We wrap the embedding molds in Saran wrap (not an off-brand) before
} polymerization.
} The blocks in blue JB4 molds hardened, but have a surface stickiness.
} The blocks in the clear molds disappeared. The black blocks of osmicated
} agar are there but the epoxy is gone.
} Does anyone have any thoughts on this?
} I really like running up nice blocks of pancreas and kidney and even
} cardiac muscle in epoxy and taking pretty pictures in the microscope but
} unfortunately no-one here is doing this nice rewarding basic work.
}
}







} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/





From: Giorgio :      giocar-at-risc990.bologna.enea.it
Date: Thu, 4 Apr 1996 15:12:50 GMT
Subject: answers to: need books on biology...

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Following my request for more details, Ms Kaye Patterson
{kayep-at-deakin.edu.au} has sent me more information about a book I think can
be very interesting for many people. For its possible general interest, I
send this message to the list.
Greetings George
----------------------------------------------------------
George,
...
A company in America called Ward's Biology has a very large
catalogue which they send to us here in Australia every year. Their address is
P.O. Box 92912
Rochester, NY 14692-9012.
For International Customers:
Fax: 716-334-6174
Phone 716-359-2502.
They have a large selection of Biological books, manuals, guides,
etc. which you would probably find useful, as well as models, equipment,
charts, microscope slides etc. etc. etc.
They have the book you are asking about available for US$65.00,
Catalogue No. 32 W 0902.
The full details of the book are:
Morholt, Evelyn and Brandwein, Paul F. (1986) A Sourcebook for the
Biological Sciences. 3rd ed.
The publisher of my 2nd ed. copy is Harcourt, Brace & World, Inc.
New York, but it was published in 1966 (and also has Alexander Joseph as a
third author), so I don't know what the publishing details for the 3rd ed. are.
The Ward's catalogue says of the book "No biology teacher should be
without this comprehensive sourcebook of techniques, procedures,
demonstrations, projects, experiments and support material. Also includes
sections on maintaining organisms, safety and chemical preparations.
Hardcover, 813 pages, with illustrations."
I can only comment about the book as a biology technician and not as
a teacher. I find it useful as the book details activities and experiments
which our Uni students do, along with the practical aspects which I need to
get the classes ready. eg. for classes looking at Protozoans,
invertebrates, etc., the book describes the best way to get material from
the field, how to culture the various groups, how to prepare material for
microscopic examination, anesthetization, staining and fixation techniques.
The book covers many areas of Biology - anatomy, cells & tissues,
photosynthesis, ingestion & digestion, transpiration, circulation,
respiration, behaviour & coordination, genetics, growth & differentiation,
evolution, and ecology. There is a section on special techniques -
Maintaining animals useful in the classroom, Growing plants useful in the
classroom and Stockroom and facilities for biology. This is the section I
use the most.
Hope you find all this useful.
Kaye





From: dbd1-at-uclink4.berkeley.edu
Date: Thu, 4 Apr 1996 09:30:43 -0800 (PST)
Subject: Sprinklers in the EM Lab

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Halon is great for stopping fires but yes, the same mechanism that
extinguishes the fire will suffocate people. Sprinklers are generally OK
but I prefer a standard A-B-C rated fire extinguisher in the scope room.
We have had "umbrellas" mounted over the scopes for the last 20 years-
aluminum tube framework covered by a heavy-duty waterproof nylon fabric.
We reside in an old building and very occasionally, plumbing accidents
result in water leaking throught the ceiling above the scope.
Regards,


Doug Davis
Staff Research Associate
Electron Microscope Facility
University of California
Berkeley, CA 94720
(510) 642-2085, fax 643-6207
dbd1-at-uclink4.berkeley.edu







From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Thu, 4 Apr 1996 12:04:35 -0600
Subject: EM and Water

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I would venture that analog instruments might be able to weather the
onslaught of water better than newer instruments with lots of sensitive
electronics. The best approach would be to contact computing centers and
see what measures are taken to protect their instrumentation.

Secondly, some sort of risk assessment needs to be considered here. What
are the odds that floods will be generated needlessly versus having a
facility or instrumentation destroyed by fire? Most instances of fires
affecting EM units that I have heard about were caused by fires originating
other than in the EM room. Perhaps sprinklers could be used in locations
other than the EM while the EM rooms could be protected by more
electronically-friendly agents (Halon, carbon dioxide, etc).

Just my thoughts .....


#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Thu, 4 Apr 1996 12:04:35 -0600
Subject: EM and Water

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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I would venture that analog instruments might be able to weather the
onslaught of water better than newer instruments with lots of sensitive
electronics. The best approach would be to contact computing centers and
see what measures are taken to protect their instrumentation.

Secondly, some sort of risk assessment needs to be considered here. What
are the odds that floods will be generated needlessly versus having a
facility or instrumentation destroyed by fire? Most instances of fires
affecting EM units that I have heard about were caused by fires originating
other than in the EM room. Perhaps sprinklers could be used in locations
other than the EM while the EM rooms could be protected by more
electronically-friendly agents (Halon, carbon dioxide, etc).

Just my thoughts .....


#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Weiming Yu :      weiming-at-LFD.physics.uiuc.edu
Date: Thu, 4 Apr 1996 12:52:38 -0600 (CST)
Subject: Re: Temperature Controller

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Hi Friends,

Thank your all for your responses. That is a great help.


Weiming Yu Ph.D.
Dept of Physics, UIUC
1110 W. Green Street
Urbana, IL 61801
w-yu-at-uiuc.edu




From: wise-at-vaxa.cis.uwosh.edu
Date: Thu, 04 Apr 1996 09:46:53 +0000
Subject: Re: Sprinklers in the EM Lab

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} David;
}
} A few years ago, we had a very bad rainstorm in Southern California. The roof
} of our R&D building (which houses the SEM) leaked, and the ceiling tile got so
} soaked that it fell in on the SEM console. When I came in the next morning,
} there was a steady stream of water pouring into the keyboard and down into the
} electronics of the SEM. Obviously, the SEM had tripped off. I unplugged it
} and
} called the vendor (Cambridge, at the time), since we were under a service
} contract. End result - we allowed it to dry for 3 days and fired it up. It
} worked fine, and has been operational ever since; no problems have been
} attributed to this incident. In summary, I believe that the risks associated
} with fire far outweigh the risks associated with water.


Yes, but rainwater is clean. I have been told (third- or fourth-hand story
by now) that it is not the water in sprinkler systems as much as the rust,
junk and (perhaps) rust inhibitors in the pipes that cause the major
damage.

Bob Wise






From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 4 Apr 1996 09:24:27 -0500 (EST)
Subject: Re: Sprinklers in the EM Lab

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} Like many of you , I have worked in EM laboratories with water (fire
} activiated) sprinklers overhead. In some cases I have successfully lobbied
} to have them removed, as I felt there was a greater danger of electrical
} shock or massive instrument damage if they tripped in error (beware the
} pipefitter). However, there is always the possibility that your EM could
} be the source of a fire that causes great damage and injury. How do people
} feel about this?
}
Dear David,
We have had one fire in an EM room--started when the LN delivery
system ran dry and a solenoid valve shorted out. There are definitely
other potential fire dangers, such as vacuum pumps. We do not have
sprinklers. I have not heard of erronious tripping of sprinkeler sys-
tems being a particular problem. All-in-all, I lean toward having a
fire supression system, but there are obvious problems with a water-
based one, even if it trips due to a real fire. Is there a better
system for areas where large voltages and/or currents are present?
Perhaps some industries have solved this problem.
Yours,
Bill Tivol




From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Thu, 4 Apr 1996 16:34:35 -0500 (EST)
Subject: Re: EM and Water

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With regard to the thread about fire extinquishers in EM suites, we were
told during our last safety inspection that we would need to get rid of
our halon system the next time it needed a charge. This was mostly
because the insurance companies would no longer cover such things. They
require springler systems. They would rather pay the for lost
equipment than the hazards of halon. Of course our safety officer had no
answer to my question of whether the school would pay the 5,000
deductible or whether I would have to pay this.

Jay
Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************





From: emccjb-at-ttuhsc.edu (Charles J. Butterick)
Date: Thu, 4 Apr 1996 08:54:30 -0600
Subject: LN2 Safety

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Greetings,
I've read with interest the comments of others questioning liquid
nitrogen safety regulations. In spite of many years of pratical experience
with the stuff and being a trained safety officer, I took some time to
examine the subject. I disagree with those who have problems with the
regulations. The regs seem to be aimed at preventing continuous contact
and the resultant injuries.
In the CRC Handbook of Laboratory Safety, 3rd edition, pages
315-317, lists all the basic precautions (face shield, impervious clothing,
etc.). For those who disagree, gloves are optional.....except, as Tivol
pointed out, when in contact with cold metal parts. The CRC actually
suggests using some sort of potholder to protect hands from the metal. The
CRC also points out that efforts should be made to prevent trapping the
cryogen. When trapped against the skin, LN2 will cause injury since the
contact is no longer momentary and becomes continuous contact. Cuffless
pants worn over the shoes/boots are reccommended, i.e.spilled LN2 that
gets trapped inside a shoe with the foot may cause injury. For those who
suggest sandals as appropriate should think about the possibility that with
a major spill, some LN2 could be trapped beneath the foot or under the
strap of a sandal, possibly causing injury. Appropriate shoes can protect
the foot for short periods. If the footwear is frozen, generally it can be
removed before injury occurs. If gloves are used, the fit should be loose
so that they may be shed quickly...ala a hockey player's actions when he
has been treated less than courteously by an opposing player. The problem
I have with faculty and staff is the use of latex gloves while using LN2.
The Leidenfrost effect can definitely cause injury if the latex freezes
against the skin. For those who would not wear appropriate gloves, have
you considered the possibility of LN2 being trapped under a watch band, or
under a ring? In a major splash, could LN2 become trapped at the beltline,
between the pants and shirt? The whole idea is to make sure that most any
cryogen does not get trapped against the skin. If you can handle LN2
safely without protection, or have done it for years without incident, more
power to you. I hope your luck holds out.
Interestingly enough, Air Liquide, a national supplier of cryogens,
also lists similar precautions in the use of liquid nitrogen and other
cryogens.
Texas Tech has (arguably) a large number of beautiful women, but
they are not the ones coming to get liquid nitrogen....and of the
individuals who do get LN2 from our lab, I would rather them come clothed.



Charles J. Butterick (Chuck)
Electron Microscopy Center
Department of Cell Biology
and Biochemistry
Texas Tech University Health
Sciences Center
3601 4th Street
Lubbock, Texas 79430

vox (806) 743-1633
fax (806) 743-1219
email emccjb-at-ttuhsc.edu or
chuck-at-micron1.lubb.ttuhsc.edu






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 4 Apr 1996 17:05:49 GMT
Subject: EMs and sprinklers

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The sprinkler next to your scope may not be the problem. We are on
the second floor, but when the sprinklers went off on the sixth floor, water
eventually worked its way down to us. Luckily this happened in the daytime
and we had enough time to cover done the scopes and computers. No one knew
how to turn off the water so there was 6 inches on the 6th floor and
ceilings collapsed on the 5th floor exactly as they are designed to do
during a fire. There was no fire.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-





From: BGIAMMARA-at-magnum.mco.edu
Date: Thu, 04 Apr 1996 11:56:26 -0500 (EST)
Subject: Sprinklers

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David,
We had a great central laboratory in the basement of the Graduate School
with a fire sprinkler above each of three electron microscopes. The Dean
worried that the sprinklers would ruin the microscopes, so they got
insurance on them and required that I keep large plastic tarps to cover
them in case the sprinklers went off. They never did, howwever...
One Saturday, we had a huge rainstorm and the Dean's secretary called
me saying their seemed to be a "little problem in the lab." I rushed over
there to find that the drains at the top of the stairs had filled with
leaves and likewise the drain at the bottom and huge amounts of water,
like a waterfall, was cascading down the steps and filling up the lab.
The building power had gone off and the emergency lighting was on in the
hallways. I called for the maintenance crew, but they were priortized
to go save "expensive" student computers in another building. So there
I was with my broom, frantically cleaning drains and pushing water, feeling
like a character in Walt Disney's Fantasia. Because of this long and
intense fight, the only thing we lost was the carpeting, papers and books
on the lowest shelf. Saved the floor tile. The minimal rust on the feet of
the EM's wasn't even noticeable. The lab was fine. Sprinklers a non-issue.
The real devastation was done by the administration, later, when a
new Dean said "What are electron microscopes good for?" eyeing the space and
salary line he could use for a new Executive Secretary. But that's another
story. Kind regards and good wishes to all, Beverly






From: BGIAMMARA-at-gemini.mco.edu
Date: Thu, 04 Apr 1996 11:56:26 -0500 (EST)
Subject: Sprinklers

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David,
We had a great central laboratory in the basement of the Graduate School
with a fire sprinkler above each of three electron microscopes. The Dean
worried that the sprinklers would ruin the microscopes, so they got
insurance on them and required that I keep large plastic tarps to cover
them in case the sprinklers went off. They never did, howwever...
One Saturday, we had a huge rainstorm and the Dean's secretary called
me saying their seemed to be a "little problem in the lab." I rushed over
there to find that the drains at the top of the stairs had filled with
leaves and likewise the drain at the bottom and huge amounts of water,
like a waterfall, was cascading down the steps and filling up the lab.
The building power had gone off and the emergency lighting was on in the
hallways. I called for the maintenance crew, but they were priortized
to go save "expensive" student computers in another building. So there
I was with my broom, frantically cleaning drains and pushing water, feeling
like a character in Walt Disney's Fantasia. Because of this long and
intense fight, the only thing we lost was the carpeting, papers and books
on the lowest shelf. Saved the floor tile. The minimal rust on the feet of
the EM's wasn't even noticeable. The lab was fine. Sprinklers a non-issue.
The real devastation was done by the administration, later, when a
new Dean said "What are electron microscopes good for?" eyeing the space and
salary line he could use for a new Executive Secretary. But that's another
story. Kind regards and good wishes to all, Beverly



--Boundary (ID JlM655DZrjy0J4/C5dhX7g)--


----- End forwarded message






From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Thu, 4 Apr 1996 13:38:04 -0600
Subject: Re: Water and the SEM

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In message {3163F416-at-hq_smtp} "Craig, Bob" writes:
}
} Good Morning All,
} Bob Citron's response to David Rothbard reminded me on an incident we had
} with an AMRAY 1000 many years ago (20?). A cooling water line on the
} diffusion pump failed and filled the Plexiglass housing of the high voltage
} supply with treated cooling water. Upon discovery me heart entered my
} throat and visions of disaster entered my mind. As in Bob's case, the
} service engineer calmly drained the water, rinsed the power supply with
} deionized water and dried it with a hair dryer. Other component also have
} been wet, which he dried with Kim Wipes. When he fired it up I expected
} sparks to fly, etc. No way!! The microscope worked like nothing had
} happened. Today the scope is 23 or 24 years old and still running! These
} experiences would indicate that older analog instruments were pretty hardy.
} How a modern digital instrument would react to such treatment is a good
} question.
} Bob Craig
} OSRAM SYLVANIA INC.
} Beverly, MA 01915
} craig-at-rd.sylvania.com


First of all, I'm very glad for Bob & Bob that water on their SEM's did not
cause any serious problems that simple drying out did not cure.

But I must relay my experience with a wet SEM lest folks get the impression that
these instuments are waterproof (no one would ever REALLY think that would
they?).

In past years, we have had water accidents in labs over our heads, in some cases
two floors above, and water has leaked down through cracks in their concrete
floors, through our ceiling, and onto our instruments (kind of like magnetic
atraction). In one case enough water got into the SEM so that a circuit board
that runs the Magnification readout and a few others were damaged a bit. Even
after replacing some transistors, the boards still blew out when powered up
again. My service engineer had to clean dirty residue left behind by the water
off the boards - many boards - with ethanol and a toothbrush to get rid of
ground paths causing electrical shorts. Naturally, such events are not covered
by service contracts, so we sent the bill upstairs to the offending lab. Some
one up there left water aspirator running all weekend, and naturally thats a
good time for paper towels to fall off the wall into the sink to plug up the
drain.

We then built plexiglass "deflectors" over our SEM and TEM, big sheets suspended
from the ceilings, to deflect any water coming down to the sides onto the floor.
Since installation in about 1985 they have saved us twice. We feel we got off
easy, as the potential was there for really serious big time damage.

The overhead panels also prevent some dust from falling onto the instruments.
And they make a great conversation piece when tours come through!

If there is any potential danger in your labs from overhead water pipes or water
from rooms above, I'd recommend that folks put up some kind of water sheding
panels. Could save a big head ache if the water ever comes down.


Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: Shin-ichi Yamane :      a0c101u-at-cc.miyazaki-u.ac.jp
Date: Fri, 05 Apr 1996 15:08:06 +0900
Subject: Re: EM: Marine invertebrate texts

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Message-Id: {9604050608.AA00087-at-S_Yamane.cc.miyazaki-u.ac.jp}

Tue, 2 Apr 1996 13:20:10 +1100
richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood) wrote.
} Dear Microscopists,
} Does anyone out there know of a textbook or any other reference material
} which covers integumental ultrastructure - cuticle, epithelium and
} associated structures and also oogenesis - in marine invertebrates. We have
} a marine science student who is studying subclass Ascothoracida who is
} having problems getting the ultrastructure info she needs.
}
} Thanks in advance.



Richard

Do you know " Comparative Animal Cytology & Histology " written by
U.Welsch and V.Storch (1976), Sidgwick & Jackson Limited, London ?
This fundamental textbook translated from Germany contains "Chapter 3.
Integument" and "Chapter 11. Reproductive organs and cells".
Descriptions, of course, cover cuticle's ultrastructures of animals
of various phyla.
I think it's an available textbook for students though had been
issued two decades ago.


S. Yamane




----------------------------------------------------
Shin-ichi Yamane [ a0c101u-at-cc.miyazaki-u.ac.jp ]
Division of Marine Bioproduction
Faculty of Agriculture, Miyazaki University,
Gakuen Kibanadai Nishi 1-1, Miyazaki 889-21, Japan
Phone : 0985-58-2811 ext.3356
----------------------------------------------------




From: Beth Trend :      trend-at-cems.umn.edu
Date: Fri, 5 Apr 1996 11:42:39 -0600
Subject: Re: Water and the SEM

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Message-Id: {199604051643.KAA22518-at-Sparc5.Microscopy.Com}

In message {199604041938.NAA17558-at-puccini.crl.umn.edu} "Gib Ahlstrand" writes:

} If there is any potential danger in your labs from overhead water pipes or
} water
} from rooms above, I'd recommend that folks put up some kind of water sheding
} panels. Could save a big head ache if the water ever comes down.
}


Sounds like a good idea ... but do you put them above or below the sprinkler
heads? :)


_______________________________________________________________________
Beth Trend trend-at-cems.umn.edu http://charfac.cie.umn.edu
Coordinator, Characterization Facility University of Minnesota
Center for Interfacial Engineering
100 Union St SE Room 187 phone 612-624-1365 fax 612-626-7530
Minneapolis, MN 55455







From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Fri, 05 Apr 1996 13:35:28 -0500 (EST)
Subject: Re: Sprinklers in the EM Lab

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In my current place of employment there are no sprinklers in the lab.
I do agree with David that they are a mixed blessing. If they do open up, there
is the possibility of damage to epquiment but in the real case of a fire,
loss of epquiment is minor compared to a loss of a life.
In my preveiously employment, the maintance folks upgraded the sprinkler
system to a water sprinkler and removed the Halon system. Their reasoning was
that a person will get wet, but not dead as with the Halon system.
If a person is worried about electrocution with the water system, all
electricty should be able to be shut off from a remote electrical substation.

Best to all,
Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu




From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 5 Apr 1996 13:37:41 -0500 (EST)
Subject: Re: SEM - Surface Area or Surface Roughness Calculations

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}
} Does anyone know of software that can calculate surface area or
} surface roughness parameters from a stereo pair obtained from an SEM?
}
Dear Dan,
If you want the true surface area--not just the projected area--
STERECON will allow you to reconstruct the volume, approximate the surface
with polygonal tiles and add these up to get an area. I don't know whether
it calculates roughness parameters, but these might be easy to determine
from the true and projected areas. I assume you are not concerned with
the fractional-dimensional properties which make the true area and the
roughness dependent on the length scale.
Yours,
Bill Tivol




From: Alan S. Pooley :      pooley-at-ahab.rutgers.edu
Date: Fri, 5 Apr 1996 15:08:47 -0500 (EST)
Subject: sprinklers

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Our building (1992) was designed with a computer room and sem room next
to each other but independantly run and served by a single sprinkler
system where the pipe is filled with air until a fire is detected by
a heat sensor (or we open a valve in a red box like a fire alarm)
then a pump fills the pipe with water but the sprinkler still has to get
hot enough to melt to start water flowing (each room independant)

Email me direct if you need me to find out details from the planners


Alan S. Pooley ,PhD Bivalve shell SEM & shape analysis
SEM/Morphometrics lab
Marine & Coastal Sciences
Rutgers University
908 932 8959 ext 225
Pooley-at-ahab.rutgers.edu





From: Margaret H Malay :      malay-at-csd.uwm.edu
Date: Fri, 5 Apr 1996 11:17:52 -0600 (CST)
Subject: Midwest Meeting

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*****************************************************
**************** !!! REMINDER !!! *****************
*****************************************************

Microscopy of Surfaces and Interfaces
Midwest Microscopy and Microanalysis Society Workshop
UWM Microscopy Open House

April 26, 1996

Please register by April 15, 1996
More information and online registration at
http://www.uwm.edu/Dept/LSS/meeting.html

PROGRAM
Golda Meir Library Conference Center
University of Wisconsin - Milwaukee

Opening Ceremonies
8:45 - 9:00

Morning Session

9:00 - 9:30
J. Mansfield
University of Michigan - Ann Arbor
"Materials Science and Biological Applications of Environmental Scanning
Electron Microscopy"

9:30-10:00
B. Tonner
University of Wisconsin - Milwaukee
"X-ray Microscopy for Chemical Analysis of Surfaces"

10:10 - 10:15 Coffee Break and Mounting of Posters
Sponsored by College of Letters and Sciences

10:15 - 10:45
T. Kelly
University of Wisconsin - Madison
"Three-Dimensional Atom Probe Microscopy"

10:45-11:15
J. Nogami
University of Wisconsin - Milwaukee
STM: "Studies of Metal Growth on Silicon Surfaces"


11:15-11:45
L. D. Marks
Northwestern University - Evanston
Surface HREM: "Old techniques on Old Surfaces - Surprising New Results"

Open House Lunch

11:45 - 1:00
Lunch sponsored by Nissei Sangyo America and Hitachi Scientific Instruments.
Poster Session

1:00 - 1:45
Poster viewing and poster competition
Poster prizes are sponsored by the MMMS.

Afternoon Session

1:45-2:15
J. M. Gibson
University of Illinois - Urbana
TEM: "All you ever wanted to know about building your own expensive and
complicated microscope, and then waiting five years until it finally works"

2:15 - 2:45
S. Babcock
University of Wisconsin - Madison
TEM/HREM: "Electron Microscopy Studies of Grain Boundaries in Bicrystals"

2:45 - 3:15
V. Dravid
Northwestern University - Evanston
"Analytical Electron Microscopy and Holography of Interfaces: Making a
Mountain out of a Mole Hill"

3:15 - 3:30 Coffee Break and Judging of Posters
Sponsored by Laboratory for Surface Studies

3:30 - 4:00
N. Browning
University of Illinois at Chicago
"High Angle Annular Dark Field in a Dedicated STEM"

4:00 - 4:30
K.L. Merkle
Argonne National Laboratory
"Relaxation Modes in Metal and Oxide Grain Boundaries"

4:30- 4:45
Poster Awards Ceremony and Closing Address
M. Gajdardziska-Josifovska, Workshop Organizer

Open House Tour

REGISTRATION FEES:
Registration is free for the members of the Midwest Microscopy and
Microanalysis Society. Members of the MicroBeam Analysis Society are members.
The workshop registration fees for non-members are same as the membership fees
and can be paid at the conference site (by check or cash):
Regular Member ($10)
Student Member ($5)

POSTERS
All lectures are by invitation only. Poster contributions by students,
researchers and microscope manufacturers are welcome. There are no size
restrictions for the posters, however they should be mounted on a sturdy
backing suitable for display on easels. The poster must be relevant for the
broad topic of microscopy of surfaces and interfaces.

POSTER COMPETITION

Graduate students are particularly encouraged to present their work and
they are eligible to enter a poster competition. One grand prize and two
first prizes will be given by the Midwest Microscopy and Microanalysis
Society. The grand prize must be used by the winner to attend a microscopy
related session at a major national/international conference.

Grand Prize: $500
1st Biological Sciences:$100
1st Physical Sciences: $100








From: Martin Kohler :      mk-at-enk.ks.se
Date: Sat, 06 Apr 1996 03:22:43 +0200
Subject: slide printers?

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High resolution slide printers?

I want to find out what slide printers (for 35mm film) there are on the
market with the following characteristics:
- generates sharp, high res, high quality slides
- has driver for Windows NT

Two apparently good slide printers are:
1. Polaroid Digital Palette HR 6000 (will have NT driver)
http://www.polaroid.com/homepage.htm
2. GALLERIA made by Mirus Industries (does not have NT drive)
http://www.mirus.com/

Could you please give any comments on these printers or suggest other slide
printers?

Thanks,
Martin

---------------
Martin K=F6hler
mk-at-enk.ks.se
---------------





From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Fri, 5 Apr 1996 19:24:48 -0500
Subject: SEM for sale

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Message-ID: {n1383385234.19715-at-mse.engin.umich.edu}

For Sale Hitachi S520 SEM.

30kV W-filament diffusion pumped microscope.
Secondary electron detector (Everhart Thornley).
BSE detector GW Electronics.
BSE detector Robinson.
S5679 Channelling Attachment.
S5010TV system.

Kevex 8000 XEDS system
10mm sq Be detector
needs new hard disk but otherwise operational.

Asking $23,000, but will consider offers.
Please contact me at the address below (email, phone, snail mail).


Jfm.


John Mansfield
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html






From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Sat, 6 Apr 1996 08:32:24 -0500
Subject: Re: Creutzfeld-Jakob disease

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Message-ID: {199604061622.LAA12559-at-IndyNet.indy.net}
To: DANIEL C VAN HART {vanhart-at-vnet.ibm.com} ,
Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

Since this disease has had a fair degree of expsoure on the TV recently can
someone describe what it is and how it could be related to the BSE disease?

Please keep it in laymans terms if you can, I am a physicist not a physician!

Many thanks.
And sorry this isn't a Microscopy question.

Jfm.



John Mansfield
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html






From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Sun, 7 Apr 1996 16:08:57 -0400 (EDT)
Subject: AREMS\SEMS 96 Meeting, Greenville SC

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JOINT AREMS/SEMS MEETING
GREENVILLE, SC

WEDNESDAY

8:00 am - 4:00 pm REGISTRATION
12:30 pm-12:45 pm OPENING REMARKS
Dennis Barr, AREMS President-Elect
Jay Jerome, SEMS President

SESSION I

12:45 pm- 1:30 pm INVITED SPEAKER
Margaret Ann Goldstein
Baylor College of Medicine

"Imaging with microscopes and computers"

1:30 pm -2:30 pm ROUNDTABLE DISCUSSION
MODERATOR: Gene Michaels, Univ of GA

"Microscopy for kids of all ages"

2:30 pm -4:30 pm POSTER SESSION

Pennington SEM evaluation of the surface of an intra-arterial sensor
retrieved from critically ill pediatric patients

Johnsrude Intraerythrocytic inclusions associated with iridoviral
infection in a fer-de-lance (Bothrops moojeni) snake

Vaughn The effects of exogenous calcium oxalate crystals on
renal epithelial cells

Vaughn Electroreceptor organ development in larval electric eels
(Electrophorus electricus)

Gambling Remote printing of large format images

Whittaker Role of electron microscopy in diagnosis of the
transmission of fibropapillomas in Green turtles,
Chelonia mydas.
5:00 pm- 6:00 pm AREMS BUSINESS MEETING AND RECEPTION

2:30 pm -8:00 pm VENDOR SESSION

4:00 pm -8:00 pm VENDOR SPONSORED TUTORIALS


THURSDAY

SESSION II

9:00 am - 9:45 am INVITED TALK
Mike Kizer
Clinch Valley College
"Life Cycle Stages of H. diminuta by SEM & Freeze Cracking"


RUSKA STUDENT COMPETITION

Abstract
No.

9:45 1 Effects of recombinant bovine somatotropin (rbST) on
satellite cell and myofiber nuclei proliferation of beef
calves. R.C. Vann.

10:00 am -10:45 am INVITED TALK
Mark Teaford
Johns Hopkins
"Analysis of rates of tooth wear via scanning electron microscopy"

10:45-11:15 am COFFEE BREAK

SESSION III

11:15 am -12:00 pm INVITED TALK
Norman Herz
University of Georgia
"Innovative uses of SEM in the determination of weathering of statues"

12:00 pm - 1:30 pm LUNCH ON YOUR OWN

SESSION IV

1:30 pm - 2:15 pm INVITED TALK
Fred Stevie
AT&T
"Focused Ion Beam application in semiconductor applications"

2:15 2 Low kV analysis of uncoated and of coated artificial root
caries. DG Gantt

3:00 3 Computer-assisted analysis of radial symmetry in airway
epithelial cilia: a new perspective on assesment of congenital
ciliary defects. JL Carson.

3:15 4 Free radical derived oxidants and endothelial cell dysfunction
in a rat model of diabetic retinopathy. E Ann Ellis.

3:30 5 Fungi associated with heating, ventilating, and air
conditioning (HVAC) systems in the southeastern united states.
RB Simmons.

3:45 6 Negative stain electron microscopy (EM) of self-assembled
gastroenteric virus capsids. CD Humphrey.

4:00 7 Ultrastructure of conida and conidium germination in the plant
pathogenic fungus Alternaria cassiae. CW Mims.

4:15 pm - 4:30 pm COFFEE BREAK

4:30 pm - 5:15 pm INVITED TALK

Locke Christman
FEI Co.
"Focused ion beam secondary ion mass spectrometry (FIB SIMS)
expands the capabilities and the applications of focused ion beam
systems."


6:00 pm - 7:00 pm SOCIAL HOUR
7:00 pm- AREMS/SEMS AWARDS BANQUET
FEATURING AN AFTER DINNER TALK BY:
Mark Teaford
Johns Hopkins
"Making teeth talk"

FRIDAY


7:30 am - 9:00 am SEMS BUSINESS BREAKFAST

SESSION V

9:00 am - 9:45 am INVITED SPEAKER

Robert Price
University of South Carolina, Medical School
"Experimental models in the study of early heart development"

9:45 8 Microanalysis in environmental science: determination of the
micro-environment of pollutants and the mechanism of
remediation of contaminated soils. TB Vander Wood.

10:00-10:40 INVITED SPEAKER
Michael M. Thomason, Diane Petrovich, Steve Myles
Analytical Services Group, Simpsonville, SC
"SEM Analysis of nonwoven fabrics used in disposable diapers and other
converted products."

10:40 9 The quantification of structure within polycrystalline fiber.
KE Robinson.

10:55-11:10 COFFEE BREAK

11:10 - 11:50 INVITED SPEAKER
Rathna Perera
" The use of electron microscopy in high performance textiles"

11:50 10 Microscopic and chemical analyses of flax and flax retting. DE
Akin.

12:05-12:45 INVITED SPEAKER
T.J. Stark, D.P. Griffis, P.E. Russell
North Carolina State University, Raleigh, NC
"H2O Enhanced focused ion beam micromachining"

12:45 CLOSING REMARKS
Mark Farmer, SEMS President-Elect
John Herr, AREMS President


For further information contact:
Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************





From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Sun, 7 Apr 1996 19:00:06 -0500
Subject: Re: Atlas of prokaryotes

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Micro people: besides requests for copies of responses to my question, here
are the responses to the question "Is there a good 1990 or later prokaryote
SEM/TEM atlas?":

} A good virus morphology reference is 'Virus Morphology', CR Madeley and AM
} Field, 2nd ed, published by Churchill Livingston, Longman UK, (1988
} however) ISBN 0-443-02784-6.
} regards,
}
} Richard Easingwood
} South Campus Electron Microscope Unit
} School of Medical Sciences
} University of Otago
} PO Box 913
} Dunedin
} NEW ZEALAND

} From: "Jane A. Fagerland (847) 935-0104" {FAGERLAND.JANE-at-igate.pprd.abbott.com}
} Subject: Re: Atlas of prokaryotes
}
} Gee, maybe we should be collecting good examples and publish a book of
} our own! Thanks.

I seems that you're right Jane, at least for the bacteria and
archea. Maybe Madeley and Field are working on a 3rd edition?
But before anyone suggests that I do it, I'm a
creepy-crawlyologist. Anyone who works on prokaryotes would look at my name
and wonder who I am.
(Aside to John Bozzola: the authors of Brock et al. _The Biology of
Micro-organisms_ are all at Southern Illinois...)
Phil

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu






From: Scott Holt :      102467.2752-at-CompuServe.COM
Date: 08 Apr 96 09:40:29 EDT
Subject: TEM Epoxy: G1

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Dear All,

Two weeks ago, I posted a message in response to questions about the composition
of GATAN's G1
TEM Epoxy. It has been brought to my attention, thankfully, that I have
mis-spoken, and should make
a corrective statement. My mistake was in assuring you that GATAN's G1 Epoxy
was the same as
EPOXY TECHNOLOGY's 353ND product. I should have stated that it was only my
personal observation
that the two are similar in characteristics, and that the MSDS sheets are
similar.

As GATAN has an interest in selling their G1 Epoxy, it was incorrect of me,
especially as we can, in the
extreme, be considered competitors, to connect their epoxy directly with EPO
TECH 353ND. However,
my purpose was only to direct you to a source for information on hazardous
components, as requested.
Please excuse my error.

MSDS information on EPO TECH 353ND can be obtained from EPOXY TECHNOLOGY by
calling:(508)667-3805.

Best Regards to all,
Scott D. Holt
BUEHLER
41 Waukegan Rd.
Lake Bluff, IL 60044
(847)295-4546





From: NORM OLSON :      NHO-at-bragg.bio.purdue.edu
Date: Mon, 8 Apr 1996 9:35:44 -0500 (EST)
Subject: Faraday cage holder

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Does anyone know of a commercial source for a TEM specimen holder
that has a Faraday cage? We are looking for one for a Philips
EM420 and related Philips scopes. I know that Gatan, Inc has
them but we were also wondering if there were any other vendors.
We would also consider a used holder if one was available.

Thanks

Norm Olson
*******************************************
* Norm Olson *
* Department of Biological Sciences *
* Lilly Hall of Life Sciences *
* Purdue University *
* West Lafayette, IN 47907 *
* *
* Phone: 317-494-5643 *
* FAX: 317-496-1189 *
* email: nho-at-bragg.bio.purdue.edu *
* *
*******************************************





From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Mon, 8 Apr 1996 10:44:50 -0400 (EDT)
Subject: 96 AREMS/SEMS meeting

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My apologies to those not interested in this meeting. I am resending this
announcement because in my first transmission I attached the wrong file.
This submission contains pertinent information about dates, registration
fees, etc. I knew I shouldn't do anything important on a Friday Afternoon.

JOINT AREMS/SEMS MEETING
GREENVILLE, SC
APRIL 24-26, 1996

LOCATION: Hyatt Regency, Greenville SC. 803-235-1234
Located in Downtown Greenville, SC
$82/night (single or double)

OTHER HOTELS WITHIN SHORT DRIVING DISTANCE:
Hampton Inn, 1-800-Hampton, 803-288-1200, $50-75
Holiday Inn, 803-297-6300, $50-75
La Quinta Inn, 1-800-531-5900, $25-50
Holiday Inn Express, 803-297-5353, $25-50

REGISTRATION FEE: $25 members/$35 non-members/$15 student (with ID) and
technicians
1 Day registration: $10

THURSDAY EVENING BANQUET: $22

You may register at the meeting. For arrangements information contact:
JoAn Hudson, EM Facillity, Clemson University, 803-656-2465

PROGRAM
WEDNESDAY

8:00 am - 4:00 pm REGISTRATION
12:30 pm-12:45 pm OPENING REMARKS
Dennis Barr, AREMS President-Elect
Jay Jerome, SEMS President

SESSION I

12:45 pm- 1:30 pm INVITED SPEAKER
Margaret Ann Goldstein
Baylor College of Medicine

"Imaging with microscopes and computers"

1:30 pm -2:30 pm ROUNDTABLE DISCUSSION
MODERATOR: Gene Michaels, Univ of GA

"Microscopy for kids of all ages"

2:30 pm -4:30 pm POSTER SESSION

Pennington SEM evaluation of the surface of an intra-arterial
sensor
retrieved from critically ill pediatric patients

Johnsrude Intraerythrocytic inclusions associated with iridoviral
infection in a fer-de-lance (Bothrops moojeni) snake

Vaughn The effects of exogenous calcium oxalate crystals on
renal epithelial cells

Vaughn Electroreceptor organ development in larval electric
eels
(Electrophorus electricus)

Gambling Remote printing of large format images

Whittaker Role of electron microscopy in diagnosis of the
transmission of fibropapillomas in Green turtles,
Chelonia mydas.
5:00 pm- 6:00 pm AREMS BUSINESS MEETING AND RECEPTION

2:30 pm -8:00 pm VENDOR SESSION

4:00 pm -8:00 pm VENDOR SPONSORED TUTORIALS


THURSDAY

SESSION II

9:00 am - 9:45 am INVITED TALK
Mike Kizer
Clinch Valley College
"Life Cycle Stages of H. diminuta by SEM & Freeze Cracking"


RUSKA STUDENT COMPETITION

Abstract
No.

9:45 1 Effects of recombinant bovine somatotropin (rbST) on
satellite cell and myofiber nuclei proliferation of beef
calves. R.C. Vann.

10:00 am -10:45 am INVITED TALK
Mark Teaford
Johns Hopkins
"Analysis of rates of tooth wear via scanning electron microscopy"

10:45-11:15 am COFFEE BREAK

SESSION III

11:15 am -12:00 pm INVITED TALK
Norman Herz
University of Georgia
"Innovative uses of SEM in the determination of weathering of statues"

12:00 pm - 1:30 pm LUNCH ON YOUR OWN

SESSION IV

1:30 pm - 2:15 pm INVITED TALK
Fred Stevie
AT&T
"Focused Ion Beam application in semiconductor applications"

2:15 2 Low kV analysis of uncoated and of coated artificial root
caries. DG Gantt

3:00 3 Computer-assisted analysis of radial symmetry in airway
epithelial cilia: a new perspective on assesment of
congenital
ciliary defects. JL Carson.

3:15 4 Free radical derived oxidants and endothelial cell
dysfunction
in a rat model of diabetic retinopathy. E Ann Ellis.

3:30 5 Fungi associated with heating, ventilating, and air
conditioning (HVAC) systems in the southeastern united
states.
RB Simmons.

3:45 6 Negative stain electron microscopy (EM) of self-assembled
gastroenteric virus capsids. CD Humphrey.

4:00 7 Ultrastructure of conida and conidium germination in the
plant
pathogenic fungus Alternaria cassiae. CW Mims.

4:15 pm - 4:30 pm COFFEE BREAK

4:30 pm - 5:15 pm INVITED TALK

Locke Christman
FEI Co.
"Focused ion beam secondary ion mass spectrometry (FIB SIMS)
expands the capabilities and the applications of focused ion beam
systems."


6:00 pm - 7:00 pm SOCIAL HOUR
7:00 pm- AREMS/SEMS AWARDS BANQUET
FEATURING AN AFTER DINNER TALK BY:
Mark Teaford
Johns Hopkins
"Making teeth talk"

FRIDAY


7:30 am - 9:00 am SEMS BUSINESS BREAKFAST

SESSION V

9:00 am - 9:45 am INVITED SPEAKER

Robert Price
University of South Carolina, Medical School
"Experimental models in the study of early heart development"

9:45 8 Microanalysis in environmental science: determination of the
micro-environment of pollutants and the mechanism of
remediation of contaminated soils. TB Vander Wood.

10:00-10:40 INVITED SPEAKER
Michael M. Thomason, Diane Petrovich, Steve Myles
Analytical Services Group, Simpsonville, SC
"SEM Analysis of nonwoven fabrics used in disposable diapers and other
converted products."

10:40 9 The quantification of structure within polycrystalline fiber.
KE Robinson.

10:55-11:10 COFFEE BREAK

11:10 - 11:50 INVITED SPEAKER
Rathna Perera
" The use of electron microscopy in high performance textiles"

11:50 10 Microscopic and chemical analyses of flax and flax retting.
DE
Akin.

12:05-12:45 INVITED SPEAKER
T.J. Stark, D.P. Griffis, P.E. Russell
North Carolina State University, Raleigh, NC
"H2O Enhanced focused ion beam micromachining"

12:45 CLOSING REMARKS
Mark Farmer, SEMS President-Elect
John Herr, AREMS President




Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************





From: akracher-at-iastate.edu (Alfred Kracher)
Date: Mon, 8 Apr 1996 10:04:06 -0600
Subject: Re: Creutzfeld-Jakob disease

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X-Sender: akracher-at-pop-1.iastate.edu
Message-Id: {v02130501ad8ee8f70559-at-[129.186.121.151]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

The article "The Prion Diseases" by Stanley B. Prusiner appeared in
Scientific American of January 1995 (vol. 272 no. 1), p.48.

Alfred Kracher
akracher-at-iastate.edu
http://www.public.iastate.edu/~akracher






From: Jane A. Fagerland (847) 935-0104 :      FAGERLAND.JANE-at-igate.pprd.abbott.com
Date: Mon, 08 Apr 1996 11:05:00 -0600 (CST)
Subject: Re: Creutzfeld-Jakob Disease

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Here's a simplified version of the mad cow disease story:

The connection between BSE and C-J Disease is that both are thought to
be caused by prions, which are self-replicating, abnormally folded,
mutated versions of a normal cellular protein. There are similar
diseases in other species, the most notable being scrapie in sheep.
The diseases have relatively long incubation periods (years) and result
in a characteristic "spongiform" lesion in the brain. The diseases are
easily diagnosed post-mortem by the presence of little holes in the
brain, which can be detected with light microscopy. Prion particles
themselves can be visualized with negative staining and TEM.

Great Britain has had a huge problem with BSE beginning in the late
1980's. The problem in beef was caused by feeding scrapie-infected
sheep scraps to cattle, and there is good evidence that the disease
jumped from sheep to cattle. The fear is that if it could make that
jump, then maybe it could jump from cattle to humans. No one has
thought to wonder, if that were the case, why it hasn't jumped from
sheep to humans, since mutton is commonly eaten in Great Britain; but
this story is not about logic - it's about sensationalism.

C-J Disease sporadically and spontaneously occurs in about 1 in a
million humans. (There is another human prion disease called kuru that
is transmitted by eating human brains - the solution to that health
problem is pretty straightforward!) Until a few weeks ago, the British
health department has been adamantly sticking to the story that there
was no evidence for an increased incidence of C-J corresponding with the
current epidemic of BSE in British cattle. However, they recently
wavered and said they couldn't rule out a BSE connection to 10 cases of
a variant form of C-J that appears to have a shorter than usual
incubation period. The British press has had a heyday with that, and I
noticed that even the Chicago Tribune had a headline in yesterday's
paper stating that an Italian had died of mad cow disease. It's not
likely that the disease would be transmitted by eating beef from
infected cattle, particularly because prions reside in central nervous
system tissues (brain and spinal cord), and humans don't normally eat
those.

The problem in Britain right now is the proverbial tempest in a teapot.
The sad part is that many farmers are losing their livelihoods because
the British press was irresponsible in the way it reported the
information from the health department.

Enough said, I guess, before I climb any higher on the soapbox!

Jane A. Fagerland, Ph.D.
Dept. of Microscopy and Microanalysis
Abbott Laboratories
Abbott Park, IL 60064






From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 8 Apr 1996 17:30:24 -0500 (EDT)
Subject: Re: NonMagAlloy

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Dear Wil,

} I am trying to design a special specimen holder for a high resolution TEM,
} and am looking for a good alloy to use for this purpose; [snip]
} one that will have sufficient strength to withstand the forces [snip],
} yet is reasonably easy to machine.
}
We have had very good luck with aluminum, believe it or not. The
machinists like it too. In the HVEM, the specimen stage is held against
the translation apparatus by 1 at. applied over ~9/16" dia., and this seems
not to affect the resolution, etc. Unless you need exact dimensions be-
tween a fiducial point on the stage and (say) the center of the specimen,
you should be able to use almost any material. If you do need the stage to
have constant dimensions to an angstrom or so, how will you mount the
specimen to this accuracy?

} I think I have heard that some special grades of stainless steel, and
} possibly some heat-resistant alloys, are suitable for use in such
} applications. Does anyone know what alloys might be candidates, or what
} limiting values of magnetic properties (permeability, susceptibility, etc.)
} an alloy should have?
}
Be very careful with stainless steels. The machining process can
convert a non-magnetic form to one with magnetism. We had the shop make
a pin for a device which ended up ~1.5" from the beam, and there was a
magnetism problem which went away when the pin was replaced by one made
of phosphor bronze--another alloy to consider if aluminum is not suitable.
Yours,
Bill Tivol




From: Mary-ann Miller - ELCE/W95 :      m8miller-at-acs.ryerson.ca
Date: Mon, 8 Apr 1996 19:24:09 -0400 (EDT)
Subject: unsubscribe

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Please unsubscribe. Thank you





From: Mary-ann Miller - ELCE/W95 :      m8miller-at-acs.ryerson.ca
Date: Mon, 8 Apr 1996 19:26:26 -0400 (EDT)
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From: Mary-ann Miller - ELCE/W95 :      m8miller-at-acs.ryerson.ca
Date: Mon, 8 Apr 1996 19:25:50 -0400 (EDT)
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From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 9 Apr 1996 10:37:58 -0400 (EDT)
Subject: STERECON

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} can you please let me know how to obtain more information about
} STERECON. Is this shareware or a commercial package?
}
Dear Hasso, et al.,
For info on STERECON, check out J. Stru. Biol. v116, pp 93-98
(1996), M. Marko & A Leith, which describes the program and its capa-
bilities, or check our Web site www.wadsworth.org under SPIDER then
STERECON. For further info, please ask the real expert, Mike Marko,
at "marko-at-wadsworth.org". He would know the particulars of the cost
of obtaining STERECON as well as any technical info.
Yours,
Bill Tivol




From: Owen P. Mills :      opmills-at-mtu.edu
Date: Tue, 9 Apr 1996 12:53:27 -0400
Subject: pulse processor

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Hi;

Can anyone confirm that a Kevex pulse processor (from a 7000 system) can be
used with a EDAX detector (with 184 pre-amp)?

Thanks,

Owen
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: dshubito-at-d.imap.itd.umich.edu (Dennis Shubitowski)
Date: Tue, 9 Apr 1996 14:50:45 -0400
Subject: Hi Res TEM Cameras

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I am looking for information on high resolution cameras that would
attach to a Philips CM100. I am familiar with Kodak's Megaplus 1.6
camera but was wondering what other manufacturers had to offer or
if Kodak has come out with anything more recent than the Megaplus.

Thanks,

Dennis Shubitowski
University of Michigan
School of Dentistry
dshubito-at-umich.edu






From: KINGSLAND, Arlene :      KINGSLAND-at-paprican.ca
Date: Tue, 9 Apr 1996 16:13:13 EST5EDT
Subject: Nanoplast

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Message-Id: {MAILQUEUE-101.960409161312.416-at-pap386.paprican.ca}

Hello/Bonjour

I am about to learn a technique which is very new to me. I will
be embedding pulp fibres in nanoplast. I have been warned that this
will tend to be very brittle for trimming.
Does anyone have any experience with this, specifically with
cellulose fibres? Is there a way to prevent the brittleness?
Any general advice...I am green at this.

Thanks, Arlene Kingsland
Pulp and Paper Research Institute of Canada






From: vickie-at-MACC.WISC.EDU
Date: Tue, 9 Apr 1996 14:19:01 -0600
Subject: Symposium/Workshop Announcement

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Symposium on:

"Integrated Microscopy"

September 20 to 22, 1996

Co-hosted by:

Integrated Microscopy Resource (IMR)
at the University of Wisconsin-Madison
an NIH Biomedical Research Resource

and

Center for Fluorescence Imaging and Biotechnology
at Carnegie-Mellon University
an NFS Biotechnology Resource

Location:

The Wisconsin Center
702 Langdon Street
Madison, WI 53706


#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*

Presentations will focus on biological problems for which a combination of
microscopies [i.e. integrated microscopy] has been used. The speakers will
demonstrate by example the power, potential and limitations of various
microscopical techniques.

#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*

This year we are happy to invite attendees to present their work during the
Saturday evening poster session. There is a limit of approx. 40 posters
which can be displayed, therefore they will be accepted on a first come
first serve basis. A one page (8.5X11) typed abstract should accompany the
registration form and fees.

#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*#*

SCHEDULE

Friday Evening, September 20, 1996
5:00- 6:55 Reception and Poster Set-up
7:00- 7:45 Alan Waggoner - Amersham Life Science/Carnegie
Mellon University
7:50- 8:35 Andrew Dixon - Biorad Microsciences
8:40- 9:25 Fred Lanni - Carnegie-Mellon University

Saturday, September 21, 1996

8:00- 8:25 Hospitality Buffet
8:30- 9:15 David Mastronarde - University of Colorado
9:20-10:05 John Z. Kiss - Miami University of Ohio
10:10-10:40 Coffee Break
10:45-11:30 Mark Ellisman - Univ. California-San Diego
11:35-12:20 Patricia Calarco - Univ. California-San Francisco
12:25- 1:25 Lunch (on your own)
1:30- 2:15 Jeff Hardin - University of Wiscosin-Madison
2:20- 3:05 Jim G. McNally - Washington University
3:10- 3:55 Enrico Gratton - Univ. of Illinois Urbana/Champaigne

4:00- 6:00 Poster Presentations
6:00- 7:00 Exhibitor's Show
7:00- 9:00 Buffet Dinner

Sunday, September 22, 1996

8:30- 8:55 Hospitality Buffet
9:00- 9:45 Steven M. Block - Princeton University
9:50-10:35 Conly Rieder - Wadsworth Center for Labs/Research
10:40-11:10 Coffee Break
11:15-12:00 Scott E. Fraser - California Institute of Technology
12:05-12:50 Phillip G. Haydon - Iowa State University

1:00- 2:00 IMR Tour

FEES:
General Registration $ 80.00
(Includes: Opening Reception, Social and Buffet Dinner, Coffee
Breaks and Materials)

Student and Local Registration $ 50.00
(Includes: Opening Reception, Social, Coffee Breaks and

Materials)

Abstract Handling Fee $ 25.00

Dinner Ticket $ 20.00


fFOR ADDITIONAL INFORMATION and PROGRAM UPDATES CONSULT OUR WEB SITE:

http://www.bocklabs.wisc.edu/imr/imr.html


TO RECEIVE A BROCHURE AND REGISTRATION FORM

WRITE: IMR
Univ. Wisconsin-Madison
1675 Observatory Drive
Madison, WI 53706

OR EMAIL: imradmin-at-calshp.cals.wisc.edu


********************************************************************************

Following the symposium, the IMR will be conducting a 2-day workshop. We
will be presenting lectures and provide "hands-on" experience for the
following techniques:
* Multiple-photon excitation imaging
* 4D DIC imaging
* Cryo-SEM
* High pressure freezing
* Reversible embeddment for SEM and TEM

Workshop attendence will be limited to 25 participants. A letter of
application is required. Once accepted a fee of $150.00 will be due.

********************************************************************************





From: Frantisek Weyda :      weyda-at-entu.cas.cz
Date: Wed, 10 Apr 1996 10:36:40 -0900 (PDT)
Subject: Re: Nanoplast

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Arlene Kingsland
Pulp and Paper Research Institute of Canada


Dear Arlene,

concerning nanoplast resins I have no experience with cellulose fibres
but I would like to recommend you my paper:

Weyda F., 1990:
Notes on the use of Nanoplast FB 101 for transmission
electron microscopy.
J.Electron Microsc.Tech., 16: 356-357

where you could find some useful information about the procedure of
embedding into Nanoplast FB 101.

Sincerely,

RNDr.Frantisek Weyda,CSc. | Frantisek Weyda, PhD.
Entomologicky ustav AV CR | Institute of Entomology
Branisovska 31
370 05 Ceske Budejovice
Ceska republika | Czech Republic
---------------------------------------------------------------------
tel.: (38) 817 linka 257 | phone: +42 38 817 ext.257
fax.: (38) 43625 | Fax.: +42 38 43625

Internet e-mail: weyda-at-entu.cas.cz







From: Karl Johnson :      kjohnson-at-haverford.edu
Date: Wed, 10 Apr 1996 10:16:49 +0000
Subject: Teaching Position Available

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Message-Id: {316B8A91.18CC-at-haverford.edu}

The Department of Molecular, Cellular and Developmental Biology at
Haverford College seeks an individual to co-teach a 7 week intensive
course in microscopic technique during the first quarter of
1996-1997 academic year. Part of the major core, this laboratory
has an expected enrollment of between 30 and 40 students and meets
in two sections over five afternoons a week. This laboratory
experience should include hands-on student experience in light,
fluorescence and electron microscopy with a focus upon cell and
tissue structure and function. Equipment used in the course
includes two Nikon Labophot fluorescent microscopes and an Hitachi
H-600 EM. The ideal candidate would have a Ph.D. (although A.B.D.
will be considered) and experience in electron microscopy who is
interested in gaining teaching experience in a liberal arts
environment.

The department is also seeking an individual to co-teach in our
introductory course "The Cellular Basis of Life" in the Spring
semester.

Interested candidates for either (or both) of these positions should
mail or fax a cover letter and curriculum vitae as soon as possible
to:

Slavica Matacic, Ph.D. or Karl Johnson, Ph.D.
smatacic-at-haverford.edu kjohnson-at-haverford.edu
(T) 610-896-1306 (T) 610-896-1305
Department of Molecular, Cellular and Developmental Biology
Haverford College 370 Lancaster Avenue
Haverford, PA 19041
(F) 610 896-4963

Additional information about the department and its program is
available via the World Wide Web at
http:www.haverford.edu/biology/welcome.html.

Haverford College is an affirmative action, equal opportunity
employer.




From: Jane A. Fagerland (847) 935-0104 :      FAGERLAND.JANE-at-igate.pprd.abbott.com
Date: Wed, 10 Apr 1996 09:18:00 -0600 (CST)
Subject: BSE and C-J Disease

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Thanks for all the comments concerning my remarks about prion diseases.
I stand corrected on my assertion that humans don't usually eat central
nervous system tissue from sheep and cattle. Several folks from the UK
enlightened me - sheep and calf brains are considered a delicacy in some
areas of the UK and are also used as fillers in hamburger and sausage.
Also, soups are made from animals parts that may still have CNS tissues
attached to them.

Someone also pointed out that it was initially thought that scrapie
could not jump from sheep to cattle; thus, there's precedent for inter-
animal transmission, regardless of what seems likely right now.

For anyone wanting more information on BSE, there is now a BSE group.
To subscribe, send an e-mail to {majordomo-at-info.aphis.usda.gov} . Leave
the subject line blank, and type {subscribe bse} in the message. There
is also a BSE news and information service on the Web at
http://www.cabi.org/


Jane A. Fagerland, Ph.D.
Dept. Microscopy and Microanalysis
Abbott Laboratories
Abbott Park IL 60064






From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Wed, 10 Apr 1996 10:17:11 -0800
Subject: SEM:acc voltage test sample

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To all:

Two related questions:

I) is there any interest in compiling a list of samples that can be used in
lab classes to readily show different principles of microscope operation?
One example of one of my test samples would be .4 um nucleopore filters
with their nice round pores for demonstrating astigmatism and how to
correct for it. Are others willing to share their ideas in this
regard--I'll be happy to compile and post a summary

II) Along these same lines, does anyone have a suggestion for a test
sample to use in my SEM laboratory class that clearly demonstrate the
effects of changing accelerating voltage? I haven't come across an easily
obtainable, reproducible sample to clearly show:

1) enhancement of surface detail at low voltage vs high votage

2) ability to view uncoated samples at low voltage without charging vs high
voltage with charging

any suggestions in this regard would be welcome

steve

Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego, CA 92182-4614
phone: (619)594-4523
fax:(619)594-5676
email:sbarlow-at-sunstroke.sdsu.edu






From: slocombe-at-wsunix.wsu.edu (Peter Slocombe)
Date: Wed, 10 Apr 1996 12:48:01 -0700
Subject: unsubscribe

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Message-Id: {9604101842.AA03291-at-super.mhv.net}
To: nestor {Microscopy-at-Sparc5.Microscopy.Com}

unsubscribe





From: Stefan.Gunnarsson-at-devbiol.uu.se (Stefan Gunnarsson)
Date: Wed, 10 Apr 1996 21:23:13 +0100
Subject: Re: Stereology

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Sorry for being a bit after the thread with this, but it would be nice to
know if there is any freeware stereology programs around. I don't need
anything very fancy but just something simple to use mainly for
demonstrations and teaching purposes.

TIA

Stefan

Stefan Gunnarsson
Microscopy & Imaging Unit, Uppsala University
Norbyvagen 18A
S-75236 Uppsala, Sweden
tel. +46 18 182638, fax. +46 18 182683






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Wed, 10 Apr 1996 10:39:30 -0600
Subject: Stereo Imaging: Anaglyphs

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I am looking for red/green glasses for viewing some anaglyphs that I have
on slides. Ted Pella sells red/blue but I don't think they will work with
my pre-existing slides.

Also, why the change from red/green to red/blue? Is this due to the "color
bombardment" phenomenon that causes a strain to some viewers or are there
other reasons?

Any newer published references re anaglyphs besides the 1980 article by
Barber and Emerson in Scanning 3:202-206?

Many thanks.


#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Wed, 10 Apr 1996 10:39:30 -0600
Subject: Stereo Imaging: Anaglyphs

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I am looking for red/green glasses for viewing some anaglyphs that I have
on slides. Ted Pella sells red/blue but I don't think they will work with
my pre-existing slides.

Also, why the change from red/green to red/blue? Is this due to the "color
bombardment" phenomenon that causes a strain to some viewers or are there
other reasons?

Any newer published references re anaglyphs besides the 1980 article by
Barber and Emerson in Scanning 3:202-206?

Many thanks.


#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: James R. Stets :      stetsjr-at-ttown.apci.com
Date: Wed, 10 Apr 1996 15:17:57 -0400 (EDT)
Subject: Re:SEM Acc. Voltage Test Sample

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On April 10, Dr. Steven Barlow asked:

(text deleted)...does anyone have a suggestion for a test
sample to use in my SEM laboratory class that clearly demonstrate the
effects of changing accelerating voltage?

A few years ago, Dr. David Joy wrote an article which appeared in some
Hitachi literature about the advantages of low-voltage imaging. He used
a common carbon-film covered TEM grid imaged at 1 or 2 kV and the same
view at 20 kV. The low-voltage image clearly showed the carbon film,
while at 20 kV, the dominant feature was the grid bars, and the carbon
film seems to disappear.

The images were obtained at low magnification (less than X1000) so you
can do this even if you don't have a FESEM.

Jim Stets
Air Products and Chemicals, Inc.
Allentown, PA
stetsjr-at-ttown.apci.com
My opinions are my own, not my employer's






From: MelanieOwl-at-aol.com
Date: Wed, 10 Apr 1996 21:06:57 -0400
Subject: Re: Safety & Liquid N2

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Just another late word on the LN2 issue. There are crogenic gloves available
which will properly protect your hands when handling liquid nitrogen or
shutting off frozen valves and handling frozen hoses. Although I agree that
sometimes safety issues don't necessary reflect common sense, having the
gloves doesn't hurt.

By the way, it snowed yesterday, again....when will we see Spring in the
Northeast?

Regards,
Melanie Behrens
Texaco Fuels and Lubricants Research
Beacon, NY
behrema -at- Texaco.com
914-838-7261




From: Robin Griffin :      rgriffin-at-eng.uab.edu
Date: Wed, 10 Apr 1996 10:12:20 -0500
Subject: SEM analysis of collagen in cartilage

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Message-ID: {c=US%a=_%p=UAB%l=ENGEM0-960410151220Z-4934-at-engem0.eng.uab.edu}

HELP!

I have a biomedical engineering student that would like to analyze the
spatial arrangement of collagen arrangement in cartilage. I'm a
materials person and have absolutely no idea how to start, how to
prepare the samples, where to look up information or if it is even
possible. The papers we have show TEM analysis but we would prefer to
start with SEM.

Can anyone out there point me in the right direction.

Robin Griffin
Electron Optics Laboratory Manager
Materials and Mechanical Engineering
The University of Alabama at Birmingham




From: Walt Bobrowski (313) 996-7814 :      bobroww-at-aa.wl.com
Date: Thu, 11 Apr 1996 08:22:45 -0400 (EDT)
Subject: Re: Stereo Imaging: Anaglyphs

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Red/Green glasses for viewing stereo images can obtained FREE from the Philips
Electron Optics web site -at- HTTP://www.peo.philips.com

Look in the subsection of 3-D images for the electronic order form.






From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Thu, 11 Apr 1996 08:26:22 GMT
Subject: Re: Stereo Imaging: Anaglyphs

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At 10:39 AM 4/10/96 -0600, you wrote:
} I am looking for red/green glasses for viewing some anaglyphs that I have
} on slides. Ted Pella sells red/blue but I don't think they will work with
} my pre-existing slides.
}
} Also, why the change from red/green to red/blue? Is this due to the "color
} bombardment" phenomenon that causes a strain to some viewers or are there
} other reasons?
}
} Any newer published references re anaglyphs besides the 1980 article by
} Barber and Emerson in Scanning 3:202-206?
}


Hi John. This question came up recently and I archived the responses
on our web page. Go to the address at the end of this message and click on
the "Tips & Tricks Wizard". You should find two links that might be useful.

1." Stereo Glasses, Red/ Green"
2. "Presentation of Stereo Pairs"

If you do not have web acess or cannot read the files, please let me
know and I will be happy to e-mail, fax, etc... them to you.

Hope this helps.




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/





From: David Garrett :      DGARRETT-at-gab.unt.edu
Date: Thu, 11 Apr 1996 10:39:09 CST6CDT
Subject: MSA printer test file

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Can someone remind me where the standard printer test file is
located. This is the file that was being printed on different
printers and then brought to the annual meetings for comparison.
Thanks,

***************************************
David Garrett "DGARRETT-at-GAB.UNT.EDU"
University of North Texas
Dept. Biological Sciences
(817)565-3964 Fax (817)565-4136
***************************************




From: keller-at-boulder.nist.gov (Bob Keller)
Date: Thu, 11 Apr 1996 08:38:03 -0600
Subject: SEM:acc voltage test sample

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Steve,

in regard to: 1) enhancement of surface detail at low voltage vs high voltage:

Try imaging a very ductile fracture surface (e.g. mild steel, etc.) for
demonstrating high vs. low keV effects. At the higher energy, the image
looks fine, but you get excessive intensity from thinner edges. At the
lower energy, that excess brightness is gone and you start to see fine
features in the edges as well as on (what you thought were) flatter
regions. The voltage effect is quite strong for comparisons between e.g.
25 keV vs. 5 keV.

in regard to: 2) ability to view uncoated samples at low voltage without
charging vs high
voltage with charging:

For viewing uncoated samples, try a ceramic, even a piece of glass. You
can get decent, uncharged imaging on SiO2 at around 1.5 to 2.0 keV. Above
that, all the charging you could want and then some.

Bob Keller
NIST
Materials Reliability Division
Boulder, CO






From: ldm3-at-apollo.numis.nwu.edu (L. D. Marks)
Date: Thu, 11 Apr 1996 14:39:04 -0500
Subject: Postdoctoral Position

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ajayan-at-hrem.mpi-stuttgart.mpg.de, aking-at-boundaries.eng.sunysb.edu,
alan_spargo-at-muwayf.uimelb.edu, b.d.hall-at-massey.ac.nz,
BABCOCK-at-coeadm.engr.wisc.edu, batson-at-watson.ibm.com, colliex-at-frsolii,
dave-at-physics.att.com, del-at-sol1.lrsm.upenn.edu,
differ-at-apollo.numis.nwu.edu, dvd-at-ruca.ua.ac.be, fmross-at-Csa2.LBL.Gov,
frchen-at-MSC.nthu.edu.tw, furukaz-at-nrim.go.jp, hobbs-at-MOLOKAI.MIT.EDU,
iijima-at-tgn.cl.nec.co.jp, john_spence-at-macmail.inre.asu.edu,
johnf-at-chem.gla.ac.uk, JSILCOX-at-msc.cornell.edu,
jws%uk.ac.bristol.pva.BITNET-at-anlvm.ctd.anl.gov, larsk-at-inorg.su.se,
maok-at-lbl.gov, mgj-at-csd.uwm.edu, MICROSCOPY-at-sparc5.microscopy.com,
mmdisko-at-erenj.com, nanotem-at-indirect.com, pabuffat-at-i2msg1.epfl.ch,
RAVEAU-at-frcpn11.in2p3.fr, Rebecca_Ai-RP3478-at-email.sps.mot.com,
rgronsky-at-garnet.berkeley.edu, Roar_Kilaas-at-macmail.lbl.gov,
SXU-at-PTD.intel.com, turner_p-at-chem.usyd.edu.au,
userrfe2-at-mts.ucs.ualberta.ca, van_tendeloo-at-ruca.ua.ac.be,
wos1-at-cam.ac.uk

Postdoctoral Position

A postdoctoral position will be available at Northwestern
University starting around August-October 1996. The position
will involve extensive use of HREM coupled with diffraction/image
simulations and crystallography on high temperature superconductors.
A strong background in electron microscopy and crystallography
is required, and strengths in the use of computers will be
important. The ability to work with others will also be very
significant, since the position will involve extensive collaborative
work.

Send CV plus the names of 3 referees with (seperately) letters
to:
L. D. Marks
Department of Materials Science
Northwestern University
Evanston, IL 60208, USA
http://risc1.numis.nwu.edu
ldm3-at-apollo.numis.nwu.edu




From: Dr. L. P. Stoter :      LPS-at-teknesis.demon.co.uk
Date: Thu, 11 Apr 1996 21:44:43 +0000
Subject: Microscopy and Analysis Services in USA

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X-Sender: (Unverified)
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Microscopy & Analysis Services in the USA


"Microscopy and Analysis" is published 6 times a year, in UK, European and
USA editions. It is widely circulated among all types of user, both
academic and commercial, in the areas of microscopy and associated
analytical techniques.

For a number of years, as a service to its readers, Microscopy and Analysis
has published in its UK edition a listing of laboratories selling their
services. The listing provides full contact details, plus a tabulation by
technique of the services offered by each laboratory, with a note on any
special capabilities or expertise.

This year, we plan to extend this feature to the USA edition. Laboratories
are invited to send in details of the services offered. Please send on a
single page, the following information:

1. Contact name, full postal address, telephone and fax numbers, and e-mail
address.
2. Indicate if your experience is in the materials or biological sciences,
or both.
3. List the instrumental techniques available in your laboratory, for
example TEM, light microscopy, EDX, Raman spectroscopy, etc.
4. Detail any special area of skill or expertise, for example, foreign body
analysis of food, cement, asbestos fibre characterisation, etc.
5. Indicate if you would be interested in special advertising facilities
linked to this feature.

The deadline for receipt of this information is 1st August, 1996. The
information will be compiled, tabulated, and published in the September
issue of Microscopy and Analysis. There is no charge to laboratories for
this service.

Information should be faxed to +44-1372-459957.


Dr. Larry Stoter
Technical Editor
Microscopy and Analysis






From: William Nicholson :      william-at-chem.gla.ac.uk
Date: Fri, 12 Apr 1996 11:32:19 +0100
Subject: Microscopy and Analysis Services in USA

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subscribe




From: BARBARA.HARTMAN-at-spcorp.com (BARBARA HARTMAN)
Date: Fri, 12 Apr 1996 08:52:46 -0400
Subject: Immunohistochemistry of Desmin and Actin

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We have been utilizing immunohistochemistry to stain for smooth muscle
actin and desmin. We have noticed that actin stains all blood vessels
but desmin does not. Has anyone else noticed this ? Is there a
better stain to differentiate between smooth muscle and endothelial
cells ?




From: Dr. Peter Steele :      70152.3105-at-CompuServe.COM
Date: 12 Apr 96 10:35:06 EDT
Subject: Negative scanners TEM

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I have followed much of the thread on negative scanners in regard to TEM
negatives. It is still unclear if many biological/medical TEM labs use negative
scanners, and if so which make & model scanner has proven to be suitable.

Printing the digitized image obtained from the negative scanner is the other
half of the equation. What printers are in use for printing scanned negatives?
I realize that viewers of digitized negatives may have different expectations
than printing digitized images from other sources.

If you have experience with scanning TEM negatives (Kodak 4489) and printing,
especially from PCs (as opposed to MACs) I would appreciate hearing from you.

Thanks!





From: knecht-at-uconnvm.uconn.edu (David Knecht)
Date: Fri, 12 Apr 1996 09:15:20 -0500
Subject: Re: Stereo Imaging: Anaglyphs

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I bought some red/blue glasses that were cheap and durable last year. I
had bought some others earlier that were crap as the lenses fell off
quickly.
The good ones were from:
Rainbow Symphony INc. 6860 Canby Ave. #120 Reseda CA 91335
800-821-5122

} At 10:39 AM 4/10/96 -0600, you wrote:
} } I am looking for red/green glasses for viewing some anaglyphs that I have
} } on slides. Ted Pella sells red/blue but I don't think they will work with
} } my pre-existing slides.
} }
} } Also, why the change from red/green to red/blue? Is this due to the "color
} } bombardment" phenomenon that causes a strain to some viewers or are there
} } other reasons?
} }
} } Any newer published references re anaglyphs besides the 1980 article by
} } Barber and Emerson in Scanning 3:202-206?
} }
}

Dr. David Knecht
Department of Molecular and Cell Biology
University of Connecticut
U-125
Storrs, CT 06269
Knecht-at-uconnvm.uconn.edu






From: Doug Cromey :      dcromey-at-ccit.arizona.edu
Date: Fri, 12 Apr 1996 08:20:20 -0700
Subject: histochemistry questions

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I apologize if you subscribe to both lists (confocal & microscopy) and
receive this twice. I wasn't sure where to send this one.

I have two histochemistry questions:

1) How does one block endogenous biotin (the tissue in question is insect)?

2) Are there "better" mounting media for sections that have had an alkaline
phosphatase reaction run on them (better than say, "permount")?

Thanks for your help.
Doug
.....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
:...................................................................:
http://www.pharm.arizona.edu/exp_path.html





From: Ulysses Garcia C. Lins :      ULINS-at-ibccf.biof.ufrj.br
Date: Fri, 12 Apr 1996 14:33:36 GMT-0300
Subject: Reprint request...

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Dear Sir,

I have read on the microscopy list, the suggestion of you paper on
Nanoplast. Please, if possible could you send me a reprint of this
article (Weyda F., 1990: Notes on the use of Nanoplast FB 101
for transmission electron microscopy. J.Electron Microsc.Tech., 16:
356-357), since I have been trying to embed magnetotactic bacteria in
Nanoplast with little success. Despite this, it is very difficult to
obtain a copy of this article, because of the absence of subscription
of J. Electron Microsc. Tech in my country.
Many thanks in advance,

Yours very truly,
Ulysses Lins

} Ulysses Lins
} Setor de Microscopia Eletronica
} Instituto de Microbiologia Prof. Paulo de Goes
} UFRJ - CCS - Cid. Universitaria
} 21949-900 - Rio de Janeiro - RJ
} Brazil
E-mail: imvuly-at-microbio.ufrj.br
you




From: Dr. Peter Steele :      70152.3105-at-CompuServe.COM
Date: Fri, 12 Apr 1996 15:10:16 -0500
Subject: Re: Negative scanners TEM

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} I have followed much of the thread on negative scanners in regard to TEM
} negatives. It is still unclear if many biological/medical TEM labs use
} negative scanners, and if so which make & model scanner has proven to be
} suitable.
} Printing the digitized image obtained from the negative scanner is the other
} half of the equation. What printers are in use for printing scanned negatives?
*******************************************************************************
1. Scanning:

We use with great success for TEM negatives an AGFA Arcus II flatbed
scanner with the new AGFA FotoTune 2.0.8 Software. The negatives are
digitized to 14-bit with linear LUTs and 300 dpi for full frame images
(overview), and at optical 1,200 dpi for detail areas, each resulting in a
file size of 1Kx1Kx16-bit TIFF. The negatives should be exposed a step more
than used for printing to assure covered high lights. The gray tone quality
is extraordinarily good when compared to 8-bit scanning or cheap 8-bit
scanners, i.e., less than 1% IR (intensity range) noise, no scan lines even
at highest contrast resolution of contrasts smaller than three intensity
steps (from 16,000 steps per pixel), linear LUT (same contrast
distributions in highlights and shadows with positive or reversed negative
acquisition mode). We routinely zoom into the data 3-5 times with a "clean"
bicubic zoom in order to display details. The image quality is certainly
-although I cannot prove it at this time- better than can be established in
the photolab with silver halide printing.

2. Printing.

I tested several printers with a 1Kx1K image. Printer performances were
compared at the level of whole page images and 10x enlargements of the
printed images (LaserJet, Lazar Print, Dye sub,). Results are available at

http://panda.uchc.edu/htklaus/DigiLab/Printing.html

In short, you have to ask how many lines per inch are printed and at how
many gray levels (not always the same as dots per inch), plain paper or
special paper, permanence of the print, printing time, printing costs. I
print 300 lpi at 256 gray levels (4,800 dpi) on plain paper in 20-45 sec
for 1-10 MB of images per page at 3 cents per page with a HP LaserJet and
enhancer board in archival quality. I use the Lazar Print board. My source
was Smart Analytical Products (301) 598-8881. You will find everything at
my page. I am testing some more printers in the same manner (Epson, and a
1200 dpi Laser printer, but the results are expected not to be very
different from the already tested printers), A plain LaserJet will be a
good start for printing images.

Conclusion: Since over two years I have not used my state of the art
photolab since digital acquisition, flatbed scanning and digital LaserJet
printing fulfill all my needs for making image reports, slide templets
(photographed with 120 roll film on a copy stand), and publication prints.
However, my image communication is vastly improved through text imbedded
image printing in reports.

Best regards Klaus.

******************************************************************************
* : *
* Klaus-Ruediger Peters, Ph.D. : WWW Home Page: *
* Director, Molecular Imaging Laboratgory : *
* Biomolecular Structure Analysis Center : Molecular Imaging Laboratory *
* University of Connecticut Health Center : http://panda.uchc.edu/ *
* 263 Farmington Ave. : htklaus/index.html *
* Farmington, CT 06030-2017; U.S.A : Differential Hysteresis *
* : Processing Demo at http:// *
* Tel: (203) 679-3977; Fax: (203) 679-1989 : panda.uchc.edu./htbit/indiv/ *
* e-mail: Peters-at-BSAC.UCHC.EDU : /software_docs/dhp.html *
* : *
****************************************************************************
**






From: kaurin-at-rmslab.rockefeller.edu
Date: Fri, 12 Apr 1996 16:42:42 EST
Subject: immunogold/proteinase K

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Does anyone have any experience using proteinase K to unmask
antigens on LRW sections? My use is for a cytoplasmic tail membrane label
using endothelial cell monolayers. What would you use to quench it?
Thanks for any suggestions!
Shelley Landon Kaurin









From: Bob Palm x8120 :      palm-at-aeetes.re.anl.gov
Date: Fri, 12 Apr 1996 16:55:07 -0500
Subject: Used SEM with EDS

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If anyone has or knows about a used SEM with EDS please contact me.

Robert Palm
Argonne National Lab
708-252-8120




From: MelanieOwl-at-aol.com
Date: Fri, 12 Apr 1996 17:25:45 -0400
Subject: Microscopy of Wax Crystals

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Hello All:
A fellow employee of mine in a different department had some interesting
questions, some of which I can answer, some of which I have no experience
with which to answer. I was hoping that some of you may have some knowledge
you could share on this topic.
These questions all relate to cloud point measurements of oils using
polarized light microscopy, with a video camera and a temperature controlled
stage. A drop of oil is placed on a slide with a cover slip and heated above
the cloud point, then cooled 0.1 deg C/minute. The cloud point is determined
as the point at which the first wax crystal forms.

Her questions were:
1) Is it more preferrable to use a polarizing scope with a full circle 360
degree rotating stage or will an X-Y scope with a polarizer and polarizing
filter work just as well?

2) What kind of magnification would be needed to see the crystals form? Would
40X be sufficient? Would a zoom binocular be preferrable to fixed lenses?

3) Would there be any advantage to using a color camera over a black and
white one? The oil is black, and contrast is the most necessary element to
detect the crystals, not a color change.

4) What type of thermal stage is required? Mettler Toledo has a $12000
system, but is such a system overkill for this purpose? Physitemp has a TS-4
thermal stage with a 90 degree bend metal bracket with cooling fluid
circulating to it. The slide fits on the flat part of the bracket. This
costs about $3500. Will this stage be capable of ramping the temperature 0.1
degree C/minute? Are there other companies which sell thermal stages?

I was also wondering if anyone knows of any references on this test method.
Thanks in advance for your help.

Regards,
Melanie Behrens
Beacon, NY
behrema -at- Texaco.com
914-838-7261





From: becks-at-sunynassau.edu (Steve Beck)
Date: Sat, 13 Apr 1996 19:23:48 -0500
Subject: Ventilation of Chemical Storage Cabinets

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Dear Microscopists,

I am seeking the assistance of anyone who can point me to any specific
references (OSHA, NIOSH) on the proper storage of chemicals. Although our
institution has made a significant investment in the purchase of approved
chemical storage cabinets for acids, corrosives, and flammables, some of
our technical staff are reluctant to move a small quantity of chemicals
from a small storage room to a large, well ventilated preparatory room.
Their concern and claim is that these storage cabinets must be vented to
the outside in some manner. The chemicals of greatest concern include some
concentrated acid and base solutions (HCl, NaOH) and organic solvents such
as acetone and petroleum ether, in four to six liter quantities each.

I have searched the OSHA web site and have located information as to the
storage of flammables. Cabinet dimensions (metal or wood) relative to the
storage of specific quantities of flammable agents are noted, however,
there is no mention of the need for external cabinet venting. I have been
unable to find any reference as to the specific storage of acids or
alkaline solutions.

In most of the labs I have encountered (including my own EM lab), chemicals
are stored in the approved cabinets *without* separate external
ventilation. I would appreciate any specific references (web sites,
publications, etc.) that you could provide on this matter since I have a
dismantled TEM that has been awaiting installation into the small storage
room that these chemicals occupy, for over six months!

Please respond directly to me and I will post a summary to the group.
Thanks in advance!




Stephen Beck
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829





From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Mon, 15 Apr 1996 08:42:46 +0100 (BST)
Subject: Re: BSE and C-J Disease

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With reference to the BSE and CJD letters. While there is of course a
very faint chance that any of us can catch the any of the rarest of
diseases in the same way that we can be struck by lightning, the chances
of getting CJ from eating beef are marginally less that winning the big
prize on the UK National Lottery. I am quite content to eat muscle beef
and I buy a lottery ticket each week. I think you Americans have long had
the right idea in the you never eat meat that has come from the "engine
room". Leaving aside the bio-medical and econo-agricultural aspects of
BSE there is of course a delicious UK political and EURO political aspect
to this sorry story

Patrick Echlin
Cambridge UKOn Wed, 10 Apr 1996, Jane A. Fagerland (847) 935-0104
wrote:

} Thanks for all the comments concerning my remarks about prion diseases.
} I stand corrected on my assertion that humans don't usually eat central
} nervous system tissue from sheep and cattle. Several folks from the UK
} enlightened me - sheep and calf brains are considered a delicacy in some
} areas of the UK and are also used as fillers in hamburger and sausage.
} Also, soups are made from animals parts that may still have CNS tissues
} attached to them.
}
} Someone also pointed out that it was initially thought that scrapie
} could not jump from sheep to cattle; thus, there's precedent for inter-
} animal transmission, regardless of what seems likely right now.
}
} For anyone wanting more information on BSE, there is now a BSE group.
} To subscribe, send an e-mail to {majordomo-at-info.aphis.usda.gov} . Leave
} the subject line blank, and type {subscribe bse} in the message. There
} is also a BSE news and information service on the Web at
} http://www.cabi.org/
}
}
} Jane A. Fagerland, Ph.D.
} Dept. Microscopy and Microanalysis
} Abbott Laboratories
} Abbott Park IL 60064
}
}
}




From: SveEn-at-pai.liu.se (Sverker Enestrom)
Date: Mon, 15 Apr 1996 11:00:15 +0200
Subject: Re: histochemistry questions

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} I apologize if you subscribe to both lists (confocal & microscopy) and
} receive this twice. I wasn't sure where to send this one.
}
} I have two histochemistry questions:
}
} 1) How does one block endogenous biotin (the tissue in question is insect)?
* You can try successive 20 min incubations of sections in 0.1% avidin
and 0.01% biotin*

} 2) Are there "better" mounting media for sections that have had an alkaline
} phosphatase reaction run on them (better than say, "permount")?
* Yes, coverslip with aqueous-based medium after counterstaining*
} Thanks for your help.
} Doug

Good luck!
Sverker

*********************************************************
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
Fax: +46 13 13 22 57
*********************************************************






From: DAVID_GANTT-at-GSVMS2.CC.GASOU.EDU (DAVID G. GANTT)
Date: Mon, 15 Apr 1996 07:30:55 -0400
Subject: B&W SLIDES OF SEM NEGS

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Message-Id: {v01530500ad97e36858ba-at-[141.165.35.119]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I USE TO USE A POSITIVE/NEGATIVE 35MM SLIDE FLIM TO MAKE POSITIVES OF SEM
NEGATIVES - DOES ANYONE IF THIS TYPE OF FLIM STILL EXISTS? IF SO PLEASE
GIVE ME ITS NUMBER AND WHO MAKES IT.

THANK YOU

Dr. David G. Gantt Phone: 1-912-681-5964
Dept. of Biology Fax: 1-912-681-0845
Landrum Box 8042 e-mail: david_gantt-at-gsvms2.cc.gasou.edu
Georgia Southern University
Statesboro, Georgia 30460-8042








From: Mary A. Molter :      molter-at-post.its.mcw.edu
Date: Mon, 15 Apr 1996 08:37:48 -0500 (CDT)
Subject: Re: Immunohistochemistry of Desmin and Actin

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Although I have worked with this antibody in Prostate without success,
Factor VIII(von Willebrand factor), may be something to try. We feel
that we didn't have success because of the lack of vascularity in the
prostate to be able to observe any kind of staining patter. I have
two references that talk about using this antibody. The first reference
using a fluorescent Ab and the second uses it in cell culture, but either
should be of some help to you.
1. Potzsch G et al Laboratory Investigation 63(6):841-852 1990

2. McGuire PG and Orkin RW Laboratory Investigation 57(1):94-105 1987.

Good luck!

Mary
Laboratory Technologist
Medical College of Wisconsin
Department of Urology
molter-at-post.its.mcw.edu


On Fri, 12 Apr 1996, BARBARA HARTMAN wrote:

} Date: Fri, 12 Apr 1996 08:52:46 -0400
} From: BARBARA HARTMAN {BARBARA.HARTMAN-at-spcorp.com}
} To: histonet-at-pathology.swmed.edu, microscopy-at-aaem.amc.anl.gov
} Subject: Immunohistochemistry of Desmin and Actin
}
} We have been utilizing immunohistochemistry to stain for smooth muscle
} actin and desmin. We have noticed that actin stains all blood vessels
} but desmin does not. Has anyone else noticed this ? Is there a
} better stain to differentiate between smooth muscle and endothelial
} cells ?
}




From: GeneXs-at-aol.com
Date: Mon, 15 Apr 1996 10:33:10 -0400
Subject: Re: Immunohistochemistry of Desmin and Actin

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the e-mail address of Dr. Sam Bowser of the Wadsworth Center in
Albany N.Y. If someone knows it, I 'd appreciate if you could mail it to me.
Sam, if your out there, please e-mail me at genexs-at-mhv.net or
genexs-at-aol.com. Thankx.
Cya,
Gene Santagada





From: kna101-at-utdallas.edu
Date: Mon, 15 Apr 1996 08:06:36 -0500 (CDT)
Subject: Re: LM: Glycol metacrylate embedding media

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Michel,

I have used JB-4 for regular histology of the mouse skull base and had
good luck with it for toluidine blue stain only. I use 15-20 ml
breakaway molds, 0.7 gm catalyst to 100 ml of soln. A, and 1.0 ml of
solution B added to the embedding mixture of 100ml soln. A/0.7 gm
catalyst. I put the molds into icewater to start the embedding
procedure. I then overfill the molds, place the specimens into the soln.,
cover the surface with parafilm-making sure to force all the air bubbles
out from under the parafilm, seal the edges of the parafilm to the molds
with a hot spatula, and place the tray of ice water/molds into a
refrigerator overnite. If heat is a problem for your or
your friend's immunohistochemisty, the operation stated above must be done
quickly, as the polymerization of JB-4 is exothermic. The next day, I
remove the bocks from the refrig. and let them stand overnite at room
tempurature before I remove the molds. You might need to let the blocks
stand for a few more days before you attempt to cut them also. I cut
mine on a ralf knife made from a glass microslide ( the cheaper the
better) and I cut them on a standard rotary microtome with a razorblade
holder to hold the ralf knives.
As I stated above, I have not been able to get good staining with any
stain other than toluidine blue. If anyone out there has
suggestions on how to get any other stain to work with this material I'd
like to know.

Karen Pawlowski
On Fri, 12 Apr 1996, Michel Deschuyteneer wrote:

} Fellow microscopists,
}
} A colleague needs to embed nasal cavities from mice for immunocytochemistry.
} His antigens are fragile and do not whistand the standard embedding
} procedure with paraffin. Cryosectioning works but would preferably be
} avoided because of the bone tissue and decalcification has also proven
} detrimental to the antigenicity of his preparations.
}
} I suggested to try glycol metacrylate but I have no hands on experience with
} this material. In addition, there is a variety of commercial kits available,
} e.g. Polysciences' Immunobed or JB4, but the differences are not exactly
} obvious to me.
} Can anyone recommend a particular preparation of this medium? What are the
} pros and cons? Is there some good alternative?
}
} Any info and/or references would be welcomed.
} Thanks a bunch in advance.
} Regards,
}
} MICHEL
}
}
} ****************************************************
} Michel Deschuyteneer, Ph.D. deschuyt-at-sbbio.be
} Scientist Electron Microscopy Laboratory
}
} SmithKline Beecham Biologicals
} Rue de l'Institut, 89 B1330 Rixensart, BELGIUM
} Tel: +32-2-656 9290 Fax: +32-2-656 8164
} ****************************************************
} Disclaimer: the opinions expressed in this
} communication are my own and do not necessarily
} reflect those of SmithKline Beecham.
} ****************************************************
}
}




From: JOLOUGHLIN-at-MAT002.MATER.IE
Date: Mon, 15 Apr 1996 16:53:36 +0000 (GMT)
Subject: SUBSCRIBE

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From: Jane A. Fagerland (847) 935-0104 :      FAGERLAND.JANE-at-igate.pprd.abbott.com
Date: Mon, 15 Apr 1996 11:51:00 -0600 (CST)
Subject: LM: staining of glycol methacrylate sections

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I have a reliable method for hematoxylin and eosin staining of GMA
sections that works well for bone and soft tissue sections. I'd be
happy to mail or fax a copy of it to anyone who's interested.

Jane A. Fagerland, Ph.D.
Dept. Microscopy and Microanalysis
Abbott Laboratories
Abbott Park IL 60064






From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Mon, 15 Apr 1996 12:21:45 -0700 (PDT)
Subject: B & W SLIDES

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In my opinion the best film for making positive slides from negatives is
Kodak Technical Pan Film 2415 (Cat No 129 7563 for 135-36 rollls
and 129 9916 for 150 ft rolls)developed in Kodak undiluted D-19 for 4
Minutes at 20 o C. You can also vary the developer and conditions in
order to adjust the contrast range--see Kodak Publication No. P-255. If
you want a positive slide from a positive image try Kodak Precision Line
film LPD4 (150ft rolls Cat No 157 8327) for a reasonable one step b & w
transparency.









From: rgwhite-at-vaxc.cc.monash.edu.au (Rosemary White)
Date: Tue, 16 Apr 1996 06:18:34 +1200
Subject: Re: B&W SLIDES OF SEM NEGS

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Both Kodak and Ilford make a continuous tone direct positive B&W 35 mm
film. I have forgotten the specific product code, something like 6330 for
the Kodak film. It's apparently what they used to use for movies.

cheers,

Rosemary White
__ /
Department of Ecology _/ \__/ \
and Evolutionary Biology / \
Monash University / Australia \
Clayton, Victoria 3168 \ ____ /
phone 61-3-9905 5670 \_/ \_*_/
fax 61-3-9905 5613 __
email rgwhite-at-vaxc.cc.monash.edu.au \/






From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Mon, 15 Apr 1996 09:33:09 -1000 (HST)
Subject: Need suggestions for TEM immuno glycoproteins

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Aloha, microscopists,

I'm trying to help someone localize polysaccharides/glycoproteins in/on a
marine alga, first by using ConA or WGA, preferably labelled with
colloidal gold. Having never used ConA or WGA, I don't have a clue as
to where to begin. Can I use any glutaraldehyde? Left to my own devices
I will start with 0.25%. Paraformaldehyde? Like 1 to 3%? We will be
harvesting and fixing on Wednesday, April 17. After they're in blocks,
I can worry about the next steps. Blocking agents? Secondary
antibodies? If anyone has an opinion on how I should proceed, I would
love to hear from them!

Mahalo from out here in the middle of the Pacific...

Tina

*****************************************
Tina (Weatherby) Carvalho *
Biological Electron Microscope Facility *
University of Hawaii *
(808) 956-6251 *
tina-at-ahi.pbrc.hawaii.edu *
http://www.pbrc.hawaii.edu/bemf/ *
*****************************************





From: Donald Lovett :      lovett-at-trenton.edu
Date: Mon, 15 Apr 1996 17:53:48 -0400 (EDT)
Subject: Re: B&W SLIDES OF SEM NEGS

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I have had great success using Kodak Technical Pan film 2415 (develop at
maximum contrast, 4 minutes in D-19) directly through negatives sitting
on a light table, using 1/2 stop bracketing.

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-trenton.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
Trenton State College, NJ 08650-4700 fax: (609) 771-2674






From: tsi-at-werple.mira.net.au (Thomson Scientific:Paul Thomson)
Date: Tue, 16 Apr 1996 10:58:30 +1000 (EST)
Subject: Thomson Scientific Instruments Web Site

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Message-ID: {199604152322.SAA29765-at-IndyNet.indy.net}
To: Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

Hello All,
For anyone who might be interested Thomson
Scientific Instruments now have a preliminary Web Site at the following URL:

http://werple.net.au/~tsi/

Please feel free to contact us for inquiries and support regarding WinEDS
and other products.


Regards and Thanks,



Paul Thomson
Technical Director
Thomson Scientific Instruments
http://werple.net.au/~tsi/






From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Mon, 15 Apr 1996 16:08:42 -0400 (EDT)
Subject: Re: B&W SLIDES OF SEM NEGS

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On Mon, 15 Apr 1996, Robert Schmitz, Biology wrote:

}
} } I USE TO USE A POSITIVE/NEGATIVE 35MM SLIDE FLIM TO MAKE POSITIVES OF SEM
} } NEGATIVES - DOES ANYONE IF THIS TYPE OF FLIM STILL EXISTS? IF SO PLEASE
} } GIVE ME ITS NUMBER AND WHO MAKES IT.
} }
} } THANK YOU
} }
} } Dr. David G. Gantt
} }
} Kodak #5302 Positive Release 35mm Film
} rschmitz-at-uwspmail.uwsp.edu
} or
} rschmitz-at-macsrv1.uwsp.edu
} (note its macsrv"one" not "el")
} Robert (Bob) J. Schmitz
} Department of Biology,
} University of Wisc. Stevens Point.
} Stevens Point, Wisconsin 54481
} ph 715-346-2420
}
David:
I used to use Tech Pan rated at ASA 125 and got beautiful slides.
Used a Nikon on automatic mode with an aperture of about f:6 on a 90mm lens.
Use a good light box.

Developed in D-19, 1:1 for 3min, fixed, washed and dried. If you use
Orbit Bath in your fix your fix time is cut to 2 min and wash 5 min.
Orbit Bath is good for printing also. Same times for prints.
For TEM slides did the same. In the past I used type 4489 EM film for my
slide. Tech Pan works rather well for making B&W slides from Negs.

Hope this helps.

Peace,
Phil Rutledge




From: Paul Webster :      paul.webster-at-yale.edu
Date: 15 Apr 1996 17:23:13 -0400
Subject: Re: immunogold/proteinase K

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Message-Id: {n1382533119.3257-at-QuickMail.Yale.edu}

Shelley Landon Kaurin writes:
" Does anyone have any experience using proteinase K to unmask
antigens on LRW sections? My use is for a cytoplasmic tail membrane label
using endothelial cell monolayers. What would you use to quench it?
Thanks for any suggestions! "

I presume that the antibody currently does not label the antigen in the LR
White. As you correctly guess, this is probably because the antibodies cannot
gain access to the antigen. However, I an not sure that treating the sections
with proteinase K will help expose the antigenic sites for you.

Firstly, the proteinase K is a protease and will not digest resins.

Secondly, if it did digest resin, how would you make sure that the proteinase K
did not digest your antigen during the incubation.

May I suggest that a better approach may be to permiabilize the cells prior to
embedding in resin, and then treating the sections with antibody, omitting any
resin digestion. The permiabilization will wash out much of the cytoplasm but
leave membrane proteins in place. Cytoplasmic tails are then accessible to
antibodies (unless they are really short and close to the membrane).

The permiabilization can be done either before or after fixation and can be as
simple as incubating living cells in hypotonic buffer, or incubating fixed cells
in detergent.

Need more? Contact me.

Paul Webster,
Center for Cell Imaging
Yale University School of Medicine






From: rgwhite-at-vaxc.cc.monash.edu.au (Rosemary White)
Date: Tue, 16 Apr 1996 09:21:41 +1200
Subject: Re: LM: Glycol metacrylate embedding media

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RE:

} } A colleague needs to embed nasal cavities from mice for immunocytochemistry.
} } His antigens are fragile and do not whistand the standard embedding
} } procedure with paraffin. Cryosectioning works but would preferably be
} } avoided because of the bone tissue and decalcification has also proven
} } detrimental to the antigenicity of his preparations.
} }
} } I suggested to try glycol metacrylate but I have no hands on experience with
} } this material. In addition, there is a variety of commercial kits available,
} } e.g. Polysciences' Immunobed or JB4, but the differences are not exactly
} } obvious to me.

} } Regards,
} }
} } MICHEL


I haven't tried JB4 or glycol methacrylate for immuno work, but have used
butyl methyl methacrylate - it works well for plant tissue - for localising
microtubules, actin and callose at least. You need to add 5-10 mM DTT to
the fixative and to the embedding resin, seems to preserve the
antigenicity. The reference is Baskin et al. 1992 Planta 187: 405-413
(though the procedure we use is modified from this), in which earlier work
is cited also. Best results are with UV polymerisation in the cold, though
some people get away with room temperature polymerisation.

good luck,

Rosemary White
__ /
Department of Ecology _/ \__/ \
and Evolutionary Biology / \
Monash University / Australia \
Clayton, Victoria 3168 \ ____ /
phone 61-3-9905 5670 \_/ \_*_/
fax 61-3-9905 5613 __
email rgwhite-at-vaxc.cc.monash.edu.au \/






From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Tue, 16 Apr 1996 12:22:21 +1100
Subject: Re: Osmium tetroxide / IGSS

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} Hello Everybody:
}
} I've received several comments recently about the fate of silver-enhanced
} gold particles (in EM) when specimens are treated with osmium tetroxide.
} The literature indicates that this process can cause a slight reduction in
} the size of the particles, probably due to re-oxidation of the deposited
} silver. However, the effect seems to be very variable: in some cases many
} of the particles disappear, or the color fades from the sections. Has
} anyone had problems with this, and has anyone found a good way to prevent
} it?
}
} Thanks,
}
} Rick Powell


Have just come back from holiday and it seems noone has answered this yet.
I do a lot of Ag-Au for EM and initially had just this problem. After
normal embedding of the Ag enhanced tissue, there would sometimes be
beautiful Ag particles, sometimes remains of Ag with holes where the
particles had been, sometimes nothing at all. The problem turned out to be
the uranyl acetate treatment. It appears (I am no chemist) that UAc
dissolves out the Ag in some way. Not using UAc cured the problem, but
obviously contrast was then hopeless. I now gold tone tissue - the Au-Ag-Au
complex is completely stable in osmium and UAc. There is an increase in the
contrast of the tissue, which may cause some people problems, especially if
high resolution is needed, but the method really works.

Gold toning: (after method of R. Arai et al 1992, Brain Res Bull 28:343-345)
after Ag enhancing, wash in water
0.05% gold chloride 10 mins/4deg
water wash
0.05% oxalic acid 2 min
water wash
1% sodium thiosulphate (freshly made) 1 hour
water wash and embed normally

Diana van Driel
Dept Ophthalmology
Sydney University
AUSTRALIA 2006






From: Richard Edward Bonshek :      RBONSHEK-at-fs2.scg.man.ac.uk
Date: Thu, 9 May 1996 02:39:53 GMT+1
Subject: Re: Need suggestions for TEM immuno glycoproteins

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Tina Carvalho {tina-at-pbrc.hawaii.edu}

} Date sent: Mon, 15 Apr 1996 09:33:09 -1000 (HST)
} From: Tina Carvalho {tina-at-pbrc.hawaii.edu}
} To: Microscopy Newsgroup {Microscopy-at-Sparc5.Microscopy.Com}
} Subject: Need suggestions for TEM immuno glycoproteins

} Aloha, microscopists,
}
} I'm trying to help someone localize polysaccharides/glycoproteins in/on a
} marine alga, first by using ConA or WGA, preferably labelled with
} colloidal gold. Having never used ConA or WGA, I don't have a clue as
} to where to begin. Can I use any glutaraldehyde? Left to my own devices
} I will start with 0.25%. Paraformaldehyde? Like 1 to 3%? We will be
} harvesting and fixing on Wednesday, April 17. After they're in blocks,
} I can worry about the next steps. Blocking agents? Secondary
} antibodies? If anyone has an opinion on how I should proceed, I would
} love to hear from them!
}
} Mahalo from out here in the middle of the Pacific...
}
} Tina
}
} *****************************************
} Tina (Weatherby) Carvalho *
} Biological Electron Microscope Facility *
} University of Hawaii *
} (808) 956-6251 *
} tina-at-ahi.pbrc.hawaii.edu *
} http://www.pbrc.hawaii.edu/bemf/ *
} *****************************************
}
---------------------------------------------------------------------

Hi,

Aldehyde fixation, even with gluteraldehyde, should not prevent
lectin binding. We've had good success with a wide range of lectins
on formaldehyde and glut. fixed human material, both LR white and
araldite embedded (we prefer LR white). To visualise bound lectin, we
used biotinylated lectin followed by gold labelled antibiotin. A
detailed method is given in:

Localisation of alpha(2,3) and alpha(2,6) linked terminal sialic acid
groups in human trabecular meshwork. SA Chapman, RE Bonshek, RW
Stodart, KR Mackenzie, D McLeod. British Journal of Ophthalmology,
1994; 78:632-637. }

We modified a method described by Slot and Geuze:

JW Slot and HJ Geuze. In: Polak JM and Varndell IM, eds.
Immunolabelling for electron microscopy. Amsterdam: Elsevier
Scientific. 1984: 129-142.

We've also visualised lectin binding (including ConA and WGA) in
resin embedded tisue at the LM level:

Glycoconjugates of the human trabecular meshwork: a lectin
histochemical study. SA Chapman, RE Bonshek, RW Stoddart, CJP Jones,
KR Mackenzie, E O'Donoghue, D McLeod. Histochemical Journal, 1995;
27:869-881.

This gives specificities, controls, etc, for a wide range of lectins.

Good luck!


Richard.









Richard Bonshek
Lecturer/Honorary Consultant in Ophthalmic Pathology
Department of Pathological Sciences
University of Manchester
Oxford Road
Manchester
M13 9PT
UK

TELEPHONE 44-161-276-5568
FAX 44-161-273-6354

E-MAIL Richard.Bonshek-at-man.ac.uk




From: rnessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Tue, 16 Apr 1996 07:57:14 -0500 (CDT)
Subject: LM stain cells prior to embeddment

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Hi,
The question has been asked of me, what stain (prior to embeddment) to
use to be able to see a cluster of cells embedded in LR white. Since post
sectioning colloidal gold labeling is to be performed, the cells have not been
osmicated. They are translucent in the polymerized block. From my
understanding, the targeted gold binding site is a glycoprotein.
Does anyone have any suggestions as to what stain to use to be able to
visualize the cells, yet not interfer with labeling?

Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu





From: ubira-at-iqm.unicamp.br (Ubirajara Pereira Rodrigues Filho)
Date: Tue, 16 Apr 1996 08:50:54 -0200
Subject: SEM: TiO2 Grain size measurement

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Hi;

Please can someone help me? I'm trying to measure the grain size of
TiO2 particles by SEM using a deposition of this particles from a 1% Renex
300 solution. Unhappiness I fail to separate the particles that are forming
agglomerates in this solution. Can someone give me some hint?

Ubirajara Pereira Rodrigues-Filho
Instituto de Quimica - UNICAMP
Campinas, SP, Brazil
e-mail: ubira-at-iqm.unicamp.br





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 16 Apr 1996 12:30:20 -0400
Subject: HowToPolish Mo

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Subject: Time: 7:25 AM
OFFICE MEMO HowToPolish Mo Date: 4/16/96

There is a graduate student in our department who has a single crystal rod of
Molybdenum. He wants to cut off thin slices, polish them so they are of
known orientation, flat, and free of surface cold-work, so he can use them
for some surface nucleation studies.

Is there anyone out there than could recommend a method for doing this that
might be accomplished in a reasonable time and with the equipment normally
found in metallography laboratories? If so, it would help him out a great
deal. Information on good, easily-controlled etchants and polishing
reagents for Mo might also be helpful.

Thanks, W. C. Bigelow (bigelow-at-umich.edu)





From: Marianne Ekwall :      Marianne.Ekwall-at-ah.slu.se
Date: Tue, 16 Apr 1996 16:39:12 +0100
Subject: Re: LM: staining of glycol methacrylate sections

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At 11.51 1996-04-15 -0600, Jane A. Fagerland (847) 935-0104 wrote:
} I have a reliable method for hematoxylin and eosin staining of GMA
} sections that works well for bone and soft tissue sections. I'd be
} happy to mail or fax a copy of it to anyone who's interested.
}
} Jane A. Fagerland, Ph.D.
} Dept. Microscopy and Microanalysis
} Abbott Laboratories
} Abbott Park IL 60064
}
Dear Dr. Fagerland I would be very glad if yuo will be so kind and
send me a mail about the staing method for GMA sections.

Thanks Marianne Ekwall
Swedish University of Agricultural Sciences
Faculty of Veterinary Medicine
Dept of Anatomy and Histology}
}
}





From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 16 Apr 1996 10:10:17 -0500
Subject: ER/Golgi-histo

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Message-ID: {n1382472539.90533-at-msmail.tmc.tulane.edu}

I need staining ER and/OR Golgi (ER best) on cultured cells with a
histochemical marker that would make the lumen electron dense. Some years
ago, I accomplished this (without wanting it) with a potassium chromate stain,
but can not remember the exact reference. If handy on somebody desk please
send a note directly to me. Gracias.

*********************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} ----} Prof. Pathology & Otolaryngology *
*http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html*
*********************************************************************




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Tue, 16 Apr 1996 13:55:55 -0500
Subject: Paraffin sectioning problem - liver

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We have a client who is fixing liver, brain, kidney and aorta with either
formalin or freshly depolymerized paraformaldehyde and embedding in
paraffin using our automated processor. Everything cuts fine except the
liver. Fixation time has run from perfusion only, perfusion + 4 hr,
perfusion + 24 hr, 4 or 24 hr immersion only. He describes the liver as
"turning to dust" as he sections it. All the other tissues (fixed and
processed at the same time) are cutting fine. Any liver histologists out
there with insight into this problem. Thanks.

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 16 Apr 96 14:00:00 EDT
Subject: Tripod Polisher Workshop Deadline

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REDUCED FEE REGISTRATION DEADLINE APRIL 30, 1996

Workshop on Tripod Polishing

Workshop Objective
This course will cover all aspects of pre-thinning and focus on final thinning
via Tripod Polishing. Due to the limited class size and the extensive hands-on
opportuinities, this course is well suited to novices as well as advanced
Tripodders. The course will include sections on:

How to do it and why should I?
What's really going on and what am I really seeing?
How to prepare small, specific area cross-sections.
The problem of wildly differing materials (eg tungsten).
Rapid preparation of TEM cross-sections.
Preparation of a wide range of materials: semiconductors, ceramics, metals,...

Hands-on Opportunity
This course will be unique in that it will provide a hands-on opportunity for
every class participant. Tripod Polishers, Polishing Wheels, and pre-thinning
equipment will be made available to all participants and actual samples will be
prepared - by the students - as part of the course. This is a great opportunity
to get your hands dirty and actually learn by doing. The instructors will walk
you through each step of the process and then let you loose on the equipment.
This course is designed to teach the Tripod Polishing technique. Silicon
samples will be provided to the students and used as the basis for the course
teaching.

Workshop Location and Dates
South Bay Technology - San Clemente, CA
Dates: Friday & Saturday - June 14-15, 1996
Friday & Saturday - October 18-19, 1996

Previous Participants (partial list)
INTEL, AMD, Motorola, LSI Logic, Conner Peripherals, Univ of Maryland, Univ of
New Mexico, UNAM (Mexico), LG Electronics (Korea), Battelle, MEMC, MVA Inc.,
Univ of Michigan, U.S. Bureau of Mines, IBM, Naval Research Lab, Purdue Univ,
Univ of Alabama, Univ of Arizona, Univ of Colorado, Univ of Wisconsin.

Class Size
Due to the intensive hands-on aspects of this course, class size will be
strictly limited to 10 participants.

Registration Fee: $795 (includes lunches and Friday night Dinner)
$695 if registration fee paid by April 30, 1996 for June
Workshop and by August 31, 1996 for October Workshop

Registration Deadline: 30 days prior to workshop

For additional Information: Monica Pflaster
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673
TEL: 800-728-2233
FAX: 714-492-1499
e-mail: sbt-at-msa.microscopy.com




Registration Form

To register for the workshop, please fill out this form and send it, with
registration fee to:

South Bay Technology, Inc.
Workshop on Tripod Polishing
1120 Via Callejon
San Clemente, CA 92673

Payment must be made in the form of a check, money order, Visa or MasterCard.
Checks must be drawn on a U.S. Bank and made payable to South Bay Technology,
Inc. Credit card orders by FAX may be sent to South Bay Technology at
714-492-1499.

Name:


Affiliation:


Address:




City: State:
Zip: Country:_________
Telephone: FAX:

e-mail:________________________

Primary sample type:




VISA MasterCard Card #_________________________________

Expiration Date________ Signature of Cardholder_________________________

Cardholder name (Please print):________________________________________

Please circle workshop you are registering for: June 14-15 October
18-19





From: brannign-at-asrr.arsusda.gov (Peggy Brannigan)
Date: Tue, 16 Apr 1996 17:04:17 -0400
Subject: TEM- help with video camera/Mac

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Hello! Our EM lab has a DAGE-MTI SIT 68 video camera attached to the TEM
and I am looking for a way to get those images into a Macintosh 8500 (Power
PC) to analyze/process with the software IMAGE. The problem is that the
SIT 68 has an oddball line scan (2:1 interlace; 875/60) so I guess I'm
looking for a PCI board for the Mac that will grab and average the images.
Even though it's an AV Mac, it won't take this signal as is. Does anyone
have any suggestions, short of buying a new camera? Or having this one set
to standard? We'd like to keep the high resolution, if possible.

Also, once I finally have images on the Mac, I'd like to print them and
quit squandering time, money , environment etc in the darkroom. I've been
looking into 8 X 10 video printers but am wondering if anyone has had any
luck with some of the higher resolution laser printers that have come out?
I only need black and white.

Like most folks, we're pretty broke so I'm trying to do this on a
shoestring. Thanks for any help you can give me!

Peggy






From: ashamin-at-brauncorp.com
Date: Tue, 16 Apr 1996 17:46:18 -0500 (CDT)
Subject: E-mail on Microscopy

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I've been receiving mail from your site, and I'd like to remove my name from
your list. I didn't sign up myself, but someone else may have. I'm not
even sure what your address is. If you want to talk to me about this, call
me at 612-683-8732. Thanks.





From: Robert414-at-aol.com
Date: Tue, 16 Apr 1996 20:48:31 -0400
Subject: KEVEX

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Does anyone know of a good INDEPENDENT KEVEX service engineer that services
the OH area? I am interested in having mapping installed on a KEVEX 8000
unit. If anyone has any suggestions please eMail me at robert414-at-aol.com.
Thanks.








Roberto
Garcia




From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Tue, 16 Apr 1996 18:13:41 -0500
Subject: Re: LM stain cells prior to embeddment

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Message-Id: {v02120d02ad99d9e0d9f7-at-[128.174.23.164]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Hi,
} The question has been asked of me, what stain (prior to embeddment) to
} use to be able to see a cluster of cells embedded in LR white. Since post
} sectioning colloidal gold labeling is to be performed, the cells have not been
} osmicated. They are translucent in the polymerized block. From my
} understanding, the targeted gold binding site is a glycoprotein.
} Does anyone have any suggestions as to what stain to use to be able to
} visualize the cells, yet not interfer with labeling?
}
} Randy Nessler
} rnessler-at-emiris.iaf.uiowa.edu

Try 0.1% Neutral Red. May need to go stronger. In water, or buffer.
Phil

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu

**** (Don't let the bastards wear you down) ***********************






From: John M. Libert :      jlibert-at-cpcug.org
Date: 4/16/96 8:01 AM
Subject: For Your Information - VIRUS

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Message-ID: {317471CF.7C3A-at-cpcug.org}

The following message was passed on to me. I have no information as to the
veracity of this report or as to the ferocity of the virus, but take note.
Maybe others have more information.

Regards,
John Libert
Rockville, MD.


_____________________________ Forward Header
________________________________


} From Tom Beneifield, GA National Guard

______________________________ Forward Header
__________________________________


Joe,

This is serious stuff; thought you might like to review note I
received:



"VIRUS ALERT" "VIRUS ALERT"


1. This virus alert pertains to you if you have access to on-line
services such as AOL, CompuServe or any other service that allows you
to download files.

2. A new TROJAN HORSE virus has emerged on the internet with the name
PKZIP300.ZIP, so named as to give the impression that this file is a
new version of the PKZIP software used to ZIP(compress) files.

3. DO NOT DOWNLOAD THIS FILE UNDER ANY CIRCUMSTANCES!

4. If you install or expand this file, the virus will wipe your hard
disk clean and will affect modems at 14.4 baud rate and higher. This
is an extremely destructive virus and there is not yet a way to
cleaning up this destructive virus.

REPEAT: DO NOT DOWNLOAD ANY FILE WITH THE NAME "PKZIP300 reqardless of
the extension.

5. This message was confirmed by MicroSoft security.

CPT BREWTON
DOIM
""

See you,

Tom




From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 16 Apr 1996 10:10:17 -0500
Subject: ER/Golgi-histo

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From: Y. Henry Sun :      mbyhsun-at-ccvax.sinica.edu.tw
Date: Wed, 17 Apr 1996 12:08:03 +0000
Subject: Zeiss CEM 902

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We have a Zeiss CEM 902 transmission EM. The LCD needs
replacement, and Zeiss is charging about US$4000. This seemed
outrageously expensive. Does anyone have experience like this?
Is this reasonable pricing? Any suggestions will be welcomed.
Please respond directly to mbyhsun-at-ccvax.sinica.edu.tw. Thanks
very much.

Henry




From: Jane A. Fagerland (847) 935-0104 :      FAGERLAND.JANE-at-igate.pprd.abbott.com
Date: Tue, 16 Apr 1996 16:26:00 -0600 (CST)
Subject: Hematoxylin and eosin staining of GMA sections

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Mr-Received: by mta RANDB; Relayed; Tue, 16 Apr 1996 16:56:01 -0600
Mr-Received: by mta MCM$RAND; Relayed; Tue, 16 Apr 1996 16:56:12 -0600
Mr-Received: by mta RANDC; Relayed; Tue, 16 Apr 1996 16:56:24 -0600
Alternate-Recipient: prohibited
Disclose-Recipients: prohibited
Content-Return: prohibited

Since I received so many requests for this procedure, I decided it would
be faster to just send it via e-mail. If you have specific questions,
e-mail or telephone me directly.

REAGENTS:
Shandon Instant Hematoxylin:
available from Shandon/Lipshaw (1-800-547-7429), catalog number
9990107. Prepare according to package instructions.

Eosin/Phloxine B:
1% aqueous eosin 50.0 ml
1% aqueous phloxine B 5.0 ml
95% ethanol 390.0 ml
glacial acetic acid 2.5 ml

Scott's Solution:
tap water 1 liter
magnesium sulfate (anhydrous) 10 g
sodium bicarbonate 2 g


PROCEDURE:

1. Filter hematoxylin solution through Whatman No. 1 filter paper.
2. Stain sections in hematoxylin - 45 minutes
3. Rinse in running tap water - 5 minutes
4. Scott's solution - 2 minutes
5. Running tap water - 5 minutes
6. 70% ethanol - 10 dips
7. Eosin/phloxine B - 20 minutes
8. 80% ethanol - 10 quick dips
9. absolute ethanol - 2 or 3 changes, 5 dips each
10. xylene - 2 changes, 5 dips each
11. mount in Permount type medium and coverslip


I've used this for bone, lung, lymph node, liver and kidney. You can
vary the times in the stain solutions, depending on your preferences.
Intensity of eosin can be controlled by changing the time in 80%
ethanol.

Good luck!

Jane A. Fagerland, Ph.D.
Dept. of Microscopy and Microanalysis
D45M/AP31
Abbott Park IL 60064






From: Brett W. Cockman :      bcockman-at-uniwa.uwa.edu.au
Date: Wed, 17 Apr 1996 13:26:16 +0700 (WAST)
Subject: Water based mountants

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X-NUPop-Charset: English

Hello all,

Could someone suggest a reliable (permanent) water - based mountant. I wish
to make wholemounts of muscle-nerve preparations which have been stored in
glycerin. Any help would be appreciated.

Brett



----------------------------------------------------------------------------
Brett W. Cockman
Technologist in Charge
School of Dentistry
University of Western Australia
Voice: (619-2205834)
Fax: (619-2213829)
e-mail; bcockman-at-uniwa.uwa.edu.au




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Wed, 17 Apr 1996 01:10:43 EDT
Subject: SEM Sample Prep/TiO2 particles

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

Ubirajara Iereira Rodrigues-Filho wrote the following:

Please can someone help me? I'm trying to measure the grain
size of TiO2 particles by SEM using a deposition of this particles from
a 1% Renex 300 solution. Unhappiness I fail to separate the particles
that are forming agglomerates in this solution. Can someone give me
some hint?



I am assuming you are talking about particle size of the TiO2 particles
and that the problem is in getting them to disperse. Let me describe
the method we have been using for more than twenty five years. It is
of unknown origin but it was taught to me by a very creative and
innovative microscopist many years ago who spent his career working at
the DuPont Experimental Station (Robert P. Schatz).

The "secret" is the magic mixture of 60% camphor/40% naphthalene which
when melted together, the two organic compounds form a eutectic which
itself melts at just a few degrees above room temperature.

The procedure is to make some of the liquid of the right composition,
e.g. 60%/40%, and then add on the order of 0.1-.0.5 wt% of the colloid
to be dispersed, in this case, TiO2. Stubborn behavior on the part of
the particles can be dealt with by way of a few minutes in an
ultrasonic shaker.

With any simple eye dropper, a drop of the liquid (containing the
particle dispersion) is put on the surface to be used as the substrate,
and since it is at room temperature, the liquid is almost
instantaneously frozen, resulting in a thin film coating on the
surface. If an SEM mount, then "surface" is the surface of the SEM
mount. If TEM studies are contemplated, a carbon coated glass slide
would be the surface. In either case, the end result is a substrate
surface covered with a thin frozen film of the eutectic system.

The interesting characteristic of the eutectic is that at room
temperature, in a vacuum evaporator using ordinary rotary pump vacuum
levels, the solid layer will sublime away (typically an over night
procedure), leaving the colloidal particles randomly scattered around
on the substrate surface. From this point, the sample can be prepared
either for SEM or TEM, depending on particle size. It has been our own
experience that for really careful studies on TiO2 particles, TEM will
give much better images from which measurements could be taken.

With regard to the camphor and naphthalene, nothing special is needed,
just ordinary off-the-shelf material of that type that is found in most
chemistry laboratory storerooms.

We have found this technique to be useful in the dispersion of a wide
range of inorganic particles or any other particle that would not be
effected by the camphor/naphthalene mixture.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Wed, 17 Apr 1996 01:10:31 EDT
Subject: LM Glycol Methacrylate GMA resins

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

On Fri, 12 Apr 1996, Michel Deschuyteneer wrote:

} Fellow microscopists,
}
} A colleague needs to embed nasal cavities from mice for
immunocytochemistry.
} His antigens are fragile and do not whistand the standard embedding
} procedure with paraffin. Cryosectioning works but would preferably be
} avoided because of the bone tissue and decalcification has also
proven
} detrimental to the antigenicity of his preparations.
}
} I suggested to try glycol metacrylate but I have no hands on
experience with
} this material. In addition, there is a variety of commercial kits
available,
} e.g. Polysciences' Immunobed or JB4, but the differences are not
exactly
} obvious to me.

} Can anyone recommend a particular preparation of this medium? What
are the
} pros and cons? Is there some good alternative?

These kinds of questions are addressed in the following two
publications:

Stirling, John W., Histochemical Journal 24, 190-206 (1992)

Gerrits, Peter O., Eppinger, Bernhard, van Good, Harry, and Horobin,
Richard W., Cells & Materials, 1, No.3, 189-198) 1991

There can be some major differences in different GMA or HMPA based kits
coming from different sources. The first of the two above papers gives
specific mention about "low acid" GMA. The exact acid level can depend
on the purification process being used, the quality of the packaging,
and of course age of the product and the conditions of storage. The
acid level can make a big difference in the quality of the final
results. For LM, the acid component shows up as much higher levels of
background staining.

Standing in the SPI Supplies exhibit booth at trade shows might not be
the most perfect of scientific methods to draw conclusions, but the
impression I get is that because GMA has a viscosity like water, when
it comes to "bone tissue", it infiltrates quite nicely. Some have told
me "better than anything else". Another advantage of GMA is that
absolutely no alcohol dehydration is needed, since the monomer acts as
its own dehydrator. This is the presumed explanation for there being a
generally greater retention of antigenicity than with other commonly
used resins. The biggest perceived negatives associated with the use
of GMA seem to be the longer than normal learning curve frequently
reported in terms of learning how to work with it the first time.
Toxicity is often times cited as a negative, however, relative to most
of the other acrylates used in microscopy, it surely is not any worse,
and some have told me it does not effect them as much. Cost is also a
factor since the GMA and HPMA kits are certainly more expensive than
some alternative resin systems.

Disclaimer: SPI Supplies is the original commercial supplier of low
acid GMA and HPMA kits for both light and electron microscopy and we
would love to see more people enjoying the benefits of low acid GMA and
low acid HPMA embedding.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: Dr. A. J. Garratt-Reed :      t.g_reed-at-fs2.mt.umist.ac.uk
Date: Wed, 17 Apr 1996 13:49:09 +0000
Subject: Re: SEM Sample Prep/TiO2 particles

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Message-Id: {199604171315.OAA14894-at-mailspool.liv.ac.uk}
Comments: Authenticated sender is {t.g_reed-at-fs2.mt.umist.ac.uk}
GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)

Referring to Chuck Garber's ingenious method for dispersing
particulates for microscopy, what are the safety/health issues (if
any) of pumping the camphor/naphthalene mixture into the atmosphere?

Tony Garratt-Reed






From: Sandra F. Zane :      sfzane-at-unccvm.uncc.edu
Date: Wed, 17 Apr 1996 10:37:21 -0500
Subject: hierarchy

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Message-Id: {2.2.16.19960417153721.21ef4c84-at-email.uncc.edu}
X-Sender: sfzane-at-email.uncc.edu
X-Mailer: Windows Eudora Pro Version 2.2 (16)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hi Everyone,
There are a couple of questions which I would like to ask those of
you who are associated with small EM labs in a university setting.
Those of you who are kind enough to respond, would you share also
the number of people served by your facility and the number of technicians
employed?

1. Do your labs have a director?

2. If so, is that director a 9 month employee or a 12 month employee?

3. Is the director a faculty member or a staff employee?

4. What are the directors responsibilities?

5. If your labs do not have a director, is there a technician who assumes a
managerial position?

6. What are the duties of the technician/manager?

I will be most appreciative for all responses.

Sandra Zane
Sandra F. Zane, EM Tech. sfzane-at-email.uncc.edu
Dept. of Biology, UNCC Ph.(704)547-4051
9201 University City Blvd. Fax (704)547-3128
Charlotte, NC 28223





From: Bede Willenbring :      Bede.Willenbring-at-hbfuller.com
Date: Wed, 17 Apr 1996 12:45:34 -0500
Subject: SEM - Sputter/Coater Evaluations

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I am in the process of replacing a sputter coater used for our SEM samples.
I am seriously considering three units: 1) Desk II with carbon yarn
evaporation accessory from Denton; 2) EFFACoater and EFFA Mark II Carbon
Coater from Ernest F. Fullam Inc; 3) SPI Module Sputter/Carbon Coating
System from SPI Supplies.

I would like to hear from anyone who has used one or more of these systems.
What did you like best, hate worst, overall performance, reliability.... If
you've used more than one, how would you compare them?

I'm considering including a thickness monitor. If you have one, do you find
it useful or a waste of money?

If you would rather not have you response included in a summary, please so
indicate.

My thanks to all who take the time to respond.
------------------------------------------------------------------------------
Bede Willenbring
H.B. Fuller Company

1200 Wolters Blvd.
Vadnais Heights, MN 55110

Email:Bede.Willenbring-at-HBFuller.com
Phone: (612) 481-3470
Fax: (612) 481-3309
------------------------------------------------------------------------------





From: Michael OKeefe :      Michael_OKeefe-at-macmail.lbl.gov
Date: 17 Apr 1996 10:53:41 U
Subject: About the PKZIP virus

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Message-Id: {n1382383916.54300-at-macmail.lbl.gov}

Subject: Time:9:59 AM
OFFICE MEMO About the PKZIP virus Date:4/17/96

Computerized microscopists:

For more information about the PKZIP300 virus, see --

http://www.nha.com/ciac6165.html

Michael A. O'Keefe
U of C, LBNL, NCEM






From: dbd1-at-uclink4.berkeley.edu
Date: Wed, 17 Apr 1996 11:17:13 -0700 (PDT)
Subject: Alternative to H&E for GMA

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To All Who Are Interested,

There is a quick alternative to H & E staining of GMA sections. This works
on sections that are 0.5 to 2 microns thick. The stain is called Lee's
methylene blue-Basic fuchsin Stain. This stain takes about 1 minute and
gives some real nice staining similar to H & E. If you want anymore info
about this, contact me at psic-at-uclink4.berkeley.edu






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 17 Apr 1996 14:16:20 -0400
Subject: RE- safety & TiO2 method

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Message-ID: {n1382371703.92132-at-mse.engin.umich.edu}

Subject: Time: 9:11 AM
OFFICE MEMO RE: safety & TiO2 method Date: 4/17/96

Camphor and naphthalene are both materials that are commonly used as moth
balls, and as such have been left laying around all over the place in
closets, drawers and storage chests in many homes throught the world for many
years. Probably the actual level of danger from them is minimal, but if you
bring the matter up, OSHA can probably find a lot of restrictions to apply
(as in the case for shipping distilled water into a laboratory).

We had a dean once who used to tell us, "If you don't want to hear the
answer you know I'll have to give, then don't ask the question!"





From: jean ross :      jeanross-at-emiris.iaf.uiowa.edu
Date: Wed, 17 Apr 1996 07:57:49 -0500 (CDT)
Subject: Re: Paraffin sectioning problem - liver

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I've gotten liver to section by facing off the block to expose the tissue
and then soaking the blocks in ice water for about an hour. If you want
to do serial sectioning, you may need to soak the block again as you cut
deeper into the block.

Hope this helps you.

Jean Ross
Central Microscopy Research Facility
Univ. of Iowa
85 EMRB
Iowa City IA 52242
(319)335-8142
Web site: http://www.uiowa.edu/~cemrf



On Tue, 16 Apr 1996, Tom Phillips wrote:

} We have a client who is fixing liver, brain, kidney and aorta with either
} formalin or freshly depolymerized paraformaldehyde and embedding in
} paraffin using our automated processor. Everything cuts fine except the
} liver. Fixation time has run from perfusion only, perfusion + 4 hr,
} perfusion + 24 hr, 4 or 24 hr immersion only. He describes the liver as
} "turning to dust" as he sections it. All the other tissues (fixed and
} processed at the same time) are cutting fine. Any liver histologists out
} there with insight into this problem. Thanks.
}
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211
} (314)-882-4712 (voice)
} (314)-882-0123 (fax)
}






From: sphicae-at-rockvax.rockefeller.edu (Eleana Sphicas)
Date: Wed, 17 Apr 1996 17:45:00 -0400 (EDT)
Subject: Re: Paraffin sectioning problem - liver

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Jill Craig {jcraig-at-unbc.edu}







From: John M. Libert :      jlibert-at-cpcug.org
Date: 4/16/96 8:01 AM
Subject: For Your Information - VIRUS

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Message-Id: {199604171809.OAA02218-at-thomas.ge.com}


} From Tom Beneifield, GA National Guard

______________________________ Forward Header
__________________________________


Joe,

This is serious stuff; thought you might like to review note I
received:



"VIRUS ALERT" "VIRUS ALERT"


1. This virus alert pertains to you if you have access to on-line
services such as AOL, CompuServe or any other service that allows you
to download files.

2. A new TROJAN HORSE virus has emerged on the internet with the name
PKZIP300.ZIP, so named as to give the impression that this file is a
new version of the PKZIP software used to ZIP(compress) files.

3. DO NOT DOWNLOAD THIS FILE UNDER ANY CIRCUMSTANCES!

4. If you install or expand this file, the virus will wipe your hard
disk clean and will affect modems at 14.4 baud rate and higher. This
is an extremely destructive virus and there is not yet a way to
cleaning up this destructive virus.

REPEAT: DO NOT DOWNLOAD ANY FILE WITH THE NAME "PKZIP300 reqardless of
the extension.

5. This message was confirmed by MicroSoft security.

CPT BREWTON
DOIM
""

See you,

Tom
----- End of forwarded message -----




From: K1JIA-at-vaxa.stevens-tech.edu
Date: Wed, 17 Apr 1996 20:03:39 -0500 (EST)
Subject: UNSUBSCRIBE

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From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Wed, 17 Apr 1996 21:27:00 EDT
Subject: TiO2 preparation

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

Tony Garratt-Reed asked the following question:

Referring to Chuck Garber's ingenious method for dispersing
particulates for microscopy, what are the safety/health issues (if any)
of pumping the camphor/naphthalene mixture into the atmosphere?


Glad you raised the issue, it was one I have not thought about for a
long time. Of course as Wil Bigelow pointed out, part of what we are
talking about is just moth balls. And since such a small quantity is
actually being sublimed, the total volume is just one drop from an eye-
dropper, whenever I have myself walked into the lab area when this was
being done, I have never even smelled the naphthalene. But to be on
the safe side, I guess good practice would suggest that pumps be vented
to the outside, right?

Of course the whole set up when preparing the eutectic should be done
under a vented hood as should any work involving organic chemicals.

But since nothing seems to be present at levels high enough to smell,
perhaps incorrectly, we have not worried about it further.

With regard to my "teacher" of the technique, Bob Schatz, who is no
longer with us, he used to say that "the best things in life are those
that come free, and the best techniques in the EM lab are those that
don't require expensive materials".
How right he was.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html
########################################################
======================================================





From: Marianne Ekwall :      Marianne.Ekwall-at-ah.slu.se
Date: Thu, 18 Apr 1996 08:15:04 +0100
Subject: Re: Alternative to H&E for GMA

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Message-Id: {199604180352.UAA27956-at-polaris.humboldt.edu}

At 11.17 1996-04-17 -0700, dbd1-at-uclink4.berkeley.edu wrote:
} To All Who Are Interested,
}
} There is a quick alternative to H & E staining of GMA sections. This works
} on sections that are 0.5 to 2 microns thick. The stain is called Lee's
} methylene blue-Basic fuchsin Stain. This stain takes about 1 minute and
} gives some real nice staining similar to H & E. If you want anymore info
} about this, contact me at psic-at-uclink4.berkeley.edu
}
} Hallo
}
Iwould like to have more info about the staining.
} Marianne Ekwall





From: MESJASZ-at-NACDH4.NAC.AC.ZA
Date: Thu, 18 Apr 1996 11:48:39 +0200
Subject: Al finder grids

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Dear All,


I am looking for Al finder grids. Does anyone know where to buy them (names,
fax/phone numbers and possibly e-mail address would be most heplful)

Many thanks in advance

Jolanta Mesjasz




From: Dr. A. J. Garratt-Reed :      t.g_reed-at-fs2.mt.umist.ac.uk
Date: Thu, 18 Apr 1996 10:43:08 +0001
Subject: Re: Boron analysis

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Comments: Authenticated sender is {t.g_reed-at-fs2.mt.umist.ac.uk}

Dear Mark

There are two issues regarding light element analysis, namely,
does the X-ray reach the detector, and is the system capable of
recording it.

Regarding the first issue, there is more to the arrival of x-rays at
the crystal than just the "thin window". What is the window made
of? A polymer window can be quite good at transmitting B x-rays,
because the boron energy is below that of the carbon absorption
edge, provided the window is not too thick. However, on older
detectors there is, I am sure, a thick layer of ice, which may be
microns thick. This will do a fine job of absorbing boron x-rays
(and any other low-energy ones, too). Modern detectors (from some
manufacturers, at least) are quite able to withstand a cycle to room
temperature and a pump-out, and at least one manufacturer fits a
small heater to the crystal to allow it to be warmed, to sublime the
ice. If you have a TN5500, though, your detector may be fairly old.

Regarding the recording of the x-rays, one major problem is
system noise, which also degrades the spectral resolution. All
manufacturers have made steady progress at reducing the noise with
the passing of the years. However, noise can also be seriously
compromised by installation peculiarities, or inadvertent ground
loops, etc. This could be especially the case on an older
installation where, perhaps, a succession of people have been
responsible for it since the original installation, so that the
special arrangements made to reduce the noise originally have been
forgotten. Some systems allow you to view the "zero" peak which
enables you to judge directly the system noise. I don't know if the
5500 does this - many systems cut the spectrum off a few channels
above zero.

Yet another problem arises because of the very low x-ray yield
from light elements. If you analysing for boron in the presence of
heavier elements, than the boron peak is superimposed on the
bremsstrahlung from the heavier material. I did a
"back-of-the-envelope" calculation once that said that in LaB6 the
boron concentration is about 4 times the minimum detactable
concentration!. This would not be such a problem if you are
analysing boron metal, but could still be an issue for boric acid.

In the SEM, of course, you will only get a tiny boron signal,
even though the counts in the rest of the spectrum may be high,
because of the very large absorption of the boron x-rays in the
sample. You can minimise the effect by operating the SEM at a very
low voltage ( you only need 600eV electrons to excite the boron), but
of course you will lose source brightness and electron intensity (or,
conversely, spatial resolution) by doing this.

Good luck!

Tony Garratt-Reed.






From: Naresh Shah :      naresh-at-service1.uky.edu
Date: Thu, 18 Apr 1996 08:23:48 -0400
Subject: Re: Boron analysis

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Message-ID: {31763454.5AAE-at-pop.uky.edu}

MARK DARUS (216) 266-2895 wrote:
}
}
} Hello,
} My EDX is a TN-5500 and I'm trying to determine if it is sensitive,
} enough to detect Boron. I have some standards, and in a moment I'm going to
} set up some boric acid and look into it. Just placing it in the chamber and
} getting the counts up, plus switching it to thin window doesn't give me
} overwhelming success from an SPI metal standard that I have.
} I'm sure there is much more to it than that. Is there any advice that you may
} have to offer. My beam current settings are 1, 5, 10, 20 & 30 KeV.
}
} Mark Darus
}
} Darus-at-cle.dnet.ge.com


A word of caution! Boric acid has high vapor pressure and will
sublime under SEM vacuum conditions contaminating your column.
I would suggest some other compound to test.

--

Naresh Shah

University of Kentucky
Consortium for Fossil Fuel Liquefaction Science (CFFLS) and
Department of Chemical and Materials Engineering (CME)
533 South Limestone Street, Room 111
Lexington, KY 40506-0043
Phone: (606)257-5119, Office: (606)257-4027 FAX: (606)257-7215
e-mail: naresh-at-pop.uky.edu




From: GeneXs-at-aol.com
Date: Thu, 18 Apr 1996 09:54:23 -0400
Subject: Microwave TEM

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Message-Id: {199604181334.JAA17785-at-ns.ge.com}

Greetings people:
Can anyone e-mail me, or post, their experiences and opinions of microwave
processing for TEM. I work in a diagnostic pathology lab. We primarily want
to speed-up embedding time; but your comments concerning any stage of
processing will be appreciated.

Gene Santagada
genexs-at-mhv.net
genexs-at-aol.com

Kalvin Electron Microscope Lab.
Lenox Hill Hospital






From: Linda Fox :      LFOX1-at-wpo.it.luc.edu
Date: Thu, 18 Apr 1996 09:59:45 -0500
Subject: dual filter for fluorescent microscopy

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Message-Id: {s1761241.084-at-wpo.it.luc.edu}
X-Mailer: Novell GroupWise 4.1

Does anyone know if there is a dual filter available for viewing FITC
and TEXAS RED at the same time? We have a Leitz Orthoplan 2. A
student is interested in purchasing this filter (perhaps used ?), if
the cost is reasonable.
Thanks .....also thanks for all the help with the Au target for
sputter coating. Linda lfox1-at-wpo.it.luc.edu





From: BARBARA.HARTMAN-at-spcorp.com (BARBARA HARTMAN)
Date: Thu, 18 Apr 1996 09:22:52 -0400
Subject: Antigen Retrieval and Desmin

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We have been using antigen retrieval with desmin immunostain on mice
tissue and are getting some nice staining but not quite what we
expected. Our literature search has not turned up anything specific
involving the antigen retrieval and desmin , does anyone know of any
references or had any experience with this scenario ?




From: Joe D Geller :      geller-at-world.std.com
Date: Thu, 18 Apr 1996 11:01:56 -0400 (EDT)
Subject: JAMP-30 Computer control system

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attn: CFILION-at-BRMVM1.VNET.IBM.COM

Your mail was returned as undeliverable.
My company manufactures replacement computer control systems for the
JAMP-30 Auger system, but not parking stages. That is available from JEOL.

You may contact Geller MicroAnalytical Laboratory
at sales-at-gellermicro.com.

Thank you,
Joe Geller




From: Joe D Geller :      geller-at-world.std.com
Date: Thu, 18 Apr 1996 11:01:56 -0400 (EDT)
Subject: JAMP-30 Computer control system

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attn: CFILION-at-BRMVM1.VNET.IBM.COM

Your mail was returned as undeliverable.
My company manufactures replacement computer control systems for the
JAMP-30 Auger system, but not parking stages. That is available from JEOL.

You may contact Geller MicroAnalytical Laboratory
at sales-at-gellermicro.com.

Thank you,
Joe Geller




From: Joseph P. Neilly 708-938-5024 :      NEILLY.JOSEPH-at-igate.pprd.abbott.com
Date: Thu, 18 Apr 1996 08:04:00 -0600 (CST)
Subject: RE: SEM - Sputter/Coater Evaluations

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Mr-Received: by mta RANDB; Relayed; Thu, 18 Apr 1996 08:08:36 -0600
Mr-Received: by mta MCM$RAND; Relayed; Thu, 18 Apr 1996 08:09:11 -0600
Mr-Received: by mta RANDB; Relayed; Thu, 18 Apr 1996 08:09:38 -0600
Alternate-Recipient: prohibited
Disclose-Recipients: prohibited
Content-Return: prohibited

Bede,

We have used a Desk II for several years and it has performed extremely
well. The only maintainence we have done is replace worn out targets
and chnage the pump oil once a year.

Joe Neilly
Abbott Laboratories
Dept. of Microscopy and Microanalysis
North Chicago, IL 60064






From: kna101-at-utdallas.edu
Date: Thu, 18 Apr 1996 08:02:00 -0500 (CDT)
Subject: Re: Paraffin sectioning problem - liver

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I was just reading a "How to" chapter about problems of this sort. The
text is A manual for Histologictechnicians, Third edition, by Ann Preece,
Little, Brown and Co. Pubs., 1972. They state the reasons for this
crumbling could be inadequate fixation or incomplete dehydration.
Sometimes the tissue in the block is just too hard. The suggest trying
to soften the tissue first by placing it in water with a little detergent
added (1/2 tsp. per 100 ml H2O) and let it soak for up to 3 hours. If
this doesn't work, you may have to deparafinize the block and refix and
dehydrate and reembedd the block. Depending on how the tissue was
originally fixed, i.e. cardiac perfusion, the liver just may have not
gotten completely exposed to the fixative. I haven't tried these tricks
myself yet so if you have any luck, let me know. Good luck.

Karen Pawlowski

On Tue, 16 Apr 1996, Tom Phillips wrote:

} We have a client who is fixing liver, brain, kidney and aorta with either
} formalin or freshly depolymerized paraformaldehyde and embedding in
} paraffin using our automated processor. Everything cuts fine except the
} liver. Fixation time has run from perfusion only, perfusion + 4 hr,
} perfusion + 24 hr, 4 or 24 hr immersion only. He describes the liver as
} "turning to dust" as he sections it. All the other tissues (fixed and
} processed at the same time) are cutting fine. Any liver histologists out
} there with insight into this problem. Thanks.
}
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211
} (314)-882-4712 (voice)
} (314)-882-0123 (fax)
}
}
}




From: Richard Beanland +44 1327 356363 :      richard.beanland-at-gecm.com
Date: Thu, 18 Apr 1996 16:29:00 +0000 (GMT)
Subject: Backscattered electron imaging

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Disclose-Recipients: prohibited

Hello All,
I have recently strted trying to do some quantitative backscattered
imaging of lead zirconium titanate (PZT). I'm getting rather puzzled by the
results and wondered if anybody had any ideas...

I naively took some papers at face value which state that backscattered
intensity is directly proportional to the mean atomic number of the material.
However, I am getting about twice the backscattered signal from PZT that I
expect. The composition has been confirmed by microprobe analysis, so I guess
that complex compounds and/or oxides behave differently from single elements
and binary metals (?)
I also see very strong differences in contrast between a nanocrystalline
phase (pyrochlore) and a large grained phase (perovskite), although both are
_supposed_ to be of the same composition. Am I actually seeing composition
differences or does the grain size have a large effect on backscattered
intensity?
Both effects are present when using a TEM (accelerating voltage 20-100kV)
and a FEGSEM (accelerating voltage 0.5-25kV).

Any ideas, references or advice would be very welcome!


Many thanks in advance,


Richard Beanland,
GMMTL
Caswell,
Towcester,
Northants NN12 8EQ
UK.





From: Kathy Walters :      kwalters-at-emiris.iaf.uiowa.edu
Date: Thu, 18 Apr 1996 15:36:45 -0500 (CDT)
Subject: resume post

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I have been asked to post this for Gary L. Van Landuyt:

OBJECTIVE:

Senior analyst position wanted.

EXPERIENCE:

15 years SEM, EDS, and WDS, operation, analysis and maintanence.
Physics instructor at a community college.
Technical writing.

Most recently employed at Bureau of Mines, Rolla, MO and Minneapolis, MN.

EDUCATION:

M.S. In physics -at- Northern Illinois University.

PROFESSIONAL AFFILIATIONS:

Microscopy Society of America
Microbeam Analysis Society
Minnesota Microscopy Society

ADDRESS:

Gary L. Van Landuyt
2730 West 66th Street Apt. 21
Richfield MN 55423-1971

PHONE:

H: 612-869-7439
W: 612-647-9322







From: Gary Login :      glogin-at-bih.harvard.edu
Date: Thu, 18 Apr 1996 14:17:21 -0400
Subject: Microwave TEM

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Dear Gene: There are several published reviews reporting how
microwave-accelerated processing methods are incorporated into routine
histopathologic practice. A recommended review is:
1. Leong, A. S.-Y. Microwave fixation and rapid processing in a large throughput
histopathology laboratory. Pathol 23: 271-273, 1991.

Reviews of microwave Fixation for TEM and LM are reported in:
1. Login, G. R., and A. M. Dvorak. Methods of microwave fixation for
microscopy. A review of research and clinical applications: 1970-1992. Prog
Histochem Cytochem 27/4: 1-127, 1994.
2. Kok, L. P., and M. E. Boon. Microwaves for microscopy. J Microsc 158:
291-322, 1990.
3. Leong, A. S.-Y. Microwave technology for morphological studies. Cell Vision
1: 278-288, 1994.

A review of microwave-accelerated embedding is in:
1. Giammara, B. Microwave embedment for light and electron microscopy using
Epoxy resins, LR White, and other polymers. Scanning 15: 82-87, 1993.


Two books describing how to get started using microwave techniques:
1. Kok, L. P., and M. E. Boon. Microwave Cookbook for Microscopists. Leyden:
Coulomb Press, 1992.
2. Login, G. R., and A. M. Dvorak. The Microwave Toolbook. A Practical Guide
for Microscopists. Boston: Beth Israel Hospital, 1994.


Gary Login (my e-mail address is at the end of this message)

In message {960418095422_472715458-at-emout13.mail.aol.com} writes:
} Greetings people:
} Can anyone e-mail me, or post, their experiences and opinions of microwave
} processing for TEM. I work in a diagnostic pathology lab. We primarily want
} to speed-up embedding time; but your comments concerning any stage of
} processing will be appreciated.
}
} Gene Santagada
} genexs-at-mhv.net
} genexs-at-aol.com
}
} Kalvin Electron Microscope Lab.
} Lenox Hill Hospital
}


Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 18 Apr 1996 12:56:27 -0400
Subject: Safety Regs & water

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Message-ID: {n1382290113.76-at-mse.engin.umich.edu}

Subject: Time: 7:21 AM
OFFICE MEMO Safety Regs & water Date: 4/18/96

Following my comments on the safety regulations and the use of champhor and
naphthalene, several people have asked if OSHA does indeed require an MSDS
for water, and apply the usual chemical safety regulations to it. I assure
you that they actually and in fact do. Here is a message on the subject I
received recently from one of my ex-students:
- - - - - - - - - - - - - - -
"At Lockheed, the chem lab had a high resolution chromotography system
requiring triple distilled water. The bottles had to be shipped with an
MSDS, they were labeled "Hazardous", and they had to be inventoried as a
"Chemical". Likewise, an NBS EDS standard of stainless steel had to be
handled as a "Hazardous Material" because it contained chromium!"
- - - - - - - - - - - - - -
Further support can be found in a column written by Mike Ryoko, a syndicated
columnist whose writings appear in many newspapers throughout the country, a
couple of days ago. In this column he referrs to a similar situation that
was called to his attention in the Chicago area. He of course makes a number
of witty comments about the situation.

There are, of course, many other examples of situations where safety
regulations have been enforced to a ridiculous end. I heard of one
laboratory where a bottle of sand, which was unfortunately had the chemical
formula SiO2 written on it, was declared a hazardous substance (presumeably
because it contained the element silicon).

We used to have a foundry in our department which used large quantities of
ordinary sand for various purposes. Again some of this material was in bags
labeled "Silica Sand", and when workers who were cleaning out the foundry
area spotted this they called in a full safety crew complete with gas masks,
sealed suits, and all related equipment, to dispose of the material. This
was done in the summer, and undoubtedly some of the workers spent their
weekends picnicking at the beach without giving the hazard of doing so a
second thought.

In a high school not far from here a student accidentally knocked over a
graduated cylinder, spilling about 100 ml of ethyl alcohol. The entire
school was closed down for the remainder of the day while the local fire
department was called in to clean up the spill. It was later learned that
several teachers celebrated the unexpected vacation by stopping at a local
bar and consuming more alcohol than was spilled at school.

These are only a few examples that I know of. There undoubtedly many more
floating around, and it would perhaps be interesting to hear about them.

I don't mean to imply that I am against appropriate safety regulations, and I
do recognize that the imposition of such regulations have been very
beneficial in protecting people in a great number of situations. However,
in some instances, largely because of the ignorance and perversity of those
who formulate and enforce these regulations, the system has been pushed to
ridiculous extremes.

In closing, I should correct myself. I said that camphor was commonly used
in moth balls. I think this is incorrect. The two common ingredients for
moth balls are, I believe, naphthalene and para-dichlorobenzene. I know that
naphthalene is still used in this way, because I recently bought some
naphthalene moth balls to evict a possum from a hole it had dug under my
front porch. However, the para-dichlorobenzene may not be so commonly used
in this way any more because it is a chlorinate phenyl compound. Camphor,
on the other hand, has been commonly used in the stuff you spread on your
lips to help heal chapping, and for years was used as a non-prescription
medicinal in such things a camphorated oil, nazal decongestants, cough
lozengers, etc.





From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Thu, 18 Apr 1996 20:07:58 EDT
Subject: Detection of Boron

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #2.10P ] --

Mark Darus wrote:

My EDX is a TN-5500 and I'm trying to determine if it is sensitive,
enough to detect Boron. I have some standards, and in a moment I'm
going to set up some boric acid and look into it. Just placing it in
the chamber and getting the counts up, plus switching it to thin window
doesn't give me overwhelming success from an SPI metal standard that I
have. I'm sure there is much more to it than that. Is there any advice
that you may have to offer. My beam current settings are 1, 5, 10, 20
& 30 KeV.
==================

There is just no better "standard" than a solid lump of pure boron! It
is inert and it won't be changed by the electron beam. Boric acid will
be unstable and as others pointed out, it will sublime causing all
kinds of other problems.

Based on our own experience fielding technical service calls, you
should check to make sure no one has inadvertently applied too thick of
a carbon coating. People don't admit to doing this but it does happen!
This has in fact happened before, with some element of frequency, where
the carbon was thick enough to absorb all exiting B x-rays. If that is
indeed the case, then read the User Manual that came with the SPI
standard for instructions for cleaning it up and returning it to a
state where you will see the x-rays. Note: Great care must be taken
or you can run the risk of damaging the electron beam lettering
opposite each standard item.

Another point: You have listed "beam currents" but these are really
accelerating voltages. You did not mention the current, but if you see
any x-ray continuum at the B (K alpha) energies, then one can assume
the detector is working. To be sure about that, check to make sure you
can see the slightly more energetic C (K alpha) from the carbon
standard.

More often than not, the problem is simply that the detector, for one
reason or another is just not "up" to it. But you should check whether
it is a transient kind of thing (e.g. ice) or something more serious.

Additionally, and no one likes to think this way, what if something
horrible did happen and something did get mixed up along the way? But
"it" still does have to be "something" and the x-rays from that
something surely should be able to be measured and the material
actually present identified.

Hope this information might be helpful to you.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:
SpiSupp-at-aol.com

########################################################
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From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Fri, 19 Apr 1996 17:49:28 +1100
Subject: EM: Reichert OMU2 to give away

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Dear Microscopists,
We have two Reichert OMU2 ultramicrotomes which we would like to give away
(either whole or whatever pieces people want) to anyone prepared to cover
freight costs.

They are mid 1960's vintage, one is complete and one is missing a few bits,
like the binoculars. They both still have their antivibration tables.
I know it is long shot, but they may be useful for someone out
there...otherwise to the tip they go.

Contact me if interested.

Yours faithfully,

Richard


Richard Easingwood
South Campus Electron Microscope Unit
School of Medical Sciences
University of Otago
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD















From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Fri, 19 Apr 1996 08:41:09 +0000
Subject: Safety Regs & water -Reply

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Message-Id: {s1775117.014-at-wpo.nerc.ac.uk}
X-Mailer: Novell GroupWise 4.1

Hello all

This is a slightly facetious input re. safety data sheets!

In the UK there is a very useful pair of publications from BDH, a chemicla
supply company (used to be known as British Drug House, I believe).
These are collcted data sheets. Yes folks, there is one for water. Here are
a few salient points which all users of the substance should bear in mind at
all times:

1. colourless liquid - maybe that implies it is rare and difficult to see if
dropped!
2. Against solubility in water: miscible in all proportions.
3. fire and explosion hazard: not applicable (thats a relief - although if it
caught fire I suppose one could foolishly attempt to extinguish with more
water?).
4. Health hazard: no significant hazard expected, may be irritating to the
eyes.
5. Toxicity: no data.
6. Carcinogenicity: no evidence of carcinogenic properties.
7. First aid - eyes: irrigate thoroughly with water(!)
8. First aid - lungs: remove from exposure.
9. First aid - skin: wash off thoroughly with soap and water (work that one
out!).
10. First aid - mouth: wash out thoroughly with water. In severe cases
obtain medical attention(!)
11.Reactive hazards: violent reaction with acyl halides, alkali and alkaline
earth metals ....
12. Spillage disposal: wear appropriate protective clothing - listed are
gloves, goggles, face shield and protective apron. Luckily, respirators are
not needed.

Have a nice day (but watch out for water raining down!).

Keith Ryan
(Local Safety Advisor - among other hats worn!)
(used to be a good EM guy - there's a cry from the heart!)
Plymouth Marine Laboratory
Citadel hill
Plymouth PL1 2PB,
England





From: AWBlackwoo-at-AOL.com
Date: Fri, 19 Apr 1996 09:53:44 -0400
Subject: Aluminum Finder Grids

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19 April 1996

Jolanta Mesjasz asked about the availability of aluminum index or finder
grids.

As some of you know, I am responsible for the laboratories of Structure
Probe, Inc., parent company of SPI Supplies. We would love to be able to
sell finder grids and other specialty products in aluminum, but we have not
been able to develop products that meet our quality requirements. The
reason, unfortunately, is very simple but also very basic.

Most TEM grids are made by electrodeposition. The copper, nickel, gold and
other precious metal grids which we and our competitors offer are plated.
While there is a lot of technology involved in the process, it is basically
very simple, and it allows control over bar width and other details to an
incredible degree; letters, numbers, fiduciary marks and other
identifications are easy compared to controlling bar width within a range of
micrometers of the nominal dimension.

For aluminum, tungsten, molybdenum, beryllium and stainless steel grids,
however, the production process uses etching. There are several problems,
but they come down to problems in the resolution of the imaging process, the
thickness of the grid material and the nature of the etching process. We
tear our hair out trying to put out product which LOOKS like a grid, with
bars, etc. Details like letters and numbers are simply beyond our capability
at this point; we do not know of a process which can produce these details at
a price anyone is willing to pay.

If a reader knows how to produce an aluminum (or other etched) finder grid,
we (and I suspect our competitors, as well) would be most happy to talk with
you about setting up production.

Andy

Andrew W. Blackwood, Ph.D.
Structure Probe, Inc.
63 Unquowa Road
Fairfield, CT 06430-5015
Ph: 1 203 254 0000
FAX: 1 203 254 2262
e-mail: AWBlackwoo-at-aol.com
WWW: http://mail.cccbi.chester.pa.us/spi/spihome.html






From: peling-at-amnh.org (Peling Melville - Interdepartmental Facilities)
Date: Fri, 19 Apr 1996 09:43:41 -0500
Subject: Re: SEM - Sputter/Coater Evaluations

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On Wednesday April 17th, Bede Willenbring writes:

} I am in the process of replacing a sputter coater used for our SEM samples.
} I am seriously considering three units: 1) Desk II with carbon yarn
} evaporation accessory from Denton; 2) EFFACoater and EFFA Mark II Carbon
} Coater from Ernest F. Fullam Inc; 3) SPI Module Sputter/Carbon Coating
} System from SPI Supplies.
}
} I would like to hear from anyone who has used one or more of these systems.
} What did you like best, hate worst, overall performance, reliability.... If
} you've used more than one, how would you compare them?
}
} I'm considering including a thickness monitor. If you have one, do you find
} it useful or a waste of money?
}
} If you would rather not have you response included in a summary, please so
} indicate.
}
} My thanks to all who take the time to respond.


We purchased a Denton Desk II with the carbon yarn evaporation accessory
acouple of years ago and have had no problems with it. The only things
that I have had to do in terms of maintenance is give it one oil change a
year, target changes, and cleaning of the glass cylinders & various
removable stage parts.

Peling Melville


--------------------------------------------------------------
Peling Fong Melville
Senior Scientific Assistant
Interdepartmental Facilities
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 U.S.A.
******************************
E-mail: peling-at-amnh.org
Work #: (212) 769-5469
FAX #: (212) 769-5495






From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Fri, 19 Apr 1996 11:59:50 -0400
Subject: Re: LM: Glycol metacrylate embedding media stainig procedures

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Message-ID: {3C80773101F70300-at-mhs.unc.edu}
In-Reply-To: {8E6A773101F70300}

In a past life Sandy Brady and I gave several workshops on GMA processing
and staining. If I remember we gave out a very comlete handout of
staining techniques. I don't know if I can easly locate a copy but there
should be some copies floating aroud somewhere. Perhaps Sandy has an
extra copy or two.
regards
bob

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: Jill Craig :      jcraig-at-unbc.edu
Date: Sat, 20 Apr 1996 09:10:12 -0700 (PDT)
Subject: sodium sulfate in SEM

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Hi,

I have been asked to image crystals of nearly pure sodium sulfate. As it
is a drying agent which contains 10 molecules of water per molecule of
Na2(SO)4 I'm a little concerned about putting it in the SEM. I have put
it in a drying oven for 2 days and I was planning to put the stub under
vacuum for a while before coating it with gold. Are there any other
precautions or preparations I should do?

Thanks for the help!

Jill




From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Fri, 19 Apr 1996 13:24:23 -0400
Subject: Re: Paraffin sectioning problem - liver

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Message-ID: {61B7773101F70300-at-mhs.unc.edu}
In-Reply-To: {FD69773101F70300}

I'm assuming that you are working with rat or mouse models. We do a lot
of rat, mouse and fish liver work in our lab. All of our histology
processing is contracted out so I had to develop SOP's for processing
and sectioning. If you want I can fax these out to you. We've had no
sectioning problems (over 5 years now) since these SOP's were written.
regards, bob

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: rnessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Fri, 19 Apr 1996 12:43:13 -0500 (CDT)
Subject: "Free" Zeiss TEM

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Hi All,
A department here at the University of Iowa contacted us about finding a
new home for their used Zeiss 10C. It has been under service contract until
just recently. Since they have decided not to renew the service contract, they
are interested in finding someone who is willing to pay for the system to be
dismantled, shipped, and reassembled. These are the only costs, as they will
give to the instrument to said party. They have pressing needs for the space
that the scope occupies, and thus are considering junking it. It would
almost be a shame to recycle a fully functional microscope......
If interested, contact Kenneth Moore (kenneth-moore-at-uiowa.edu) or myself
either by email or phone (319-335-8142).

Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu






From: GANTZ-at-med-biophd.bu.edu
Date: Fri, 19 Apr 1996 12:19:59 -0400 (EDT)
Subject: Fire Safety

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I think that the fire safety issue is important enough to continue
the thread. An incident happened in my building last week which reminded
us of the importance of smoke detectors. An instrument which had been
left running and unattended many times in the past began to melt down
when on this occasion was left unattended for 15 minutes. Part of the
instrument became red hot, melted a hole in the bench top, and sent thick,
black, acrid smoke into the room and two adjacent ones. Directly above
the instrument are located air intake and exhaust vents which pulled
some smoke and presumably heat out of the room. The heat activated
sprinkler (designed to be activated at 135F, we think) did not activate,
presumably because heat was exhausted away. Fortunately, a student
returned to discover the situation.
Later we extracted the following information from the large
gathering which included 7 firemen, several safety and facilities
management personnel: smoke detectors are not required under current
fire safety codes in this city. Our building is two years old. The
nearest smoke detector was approx. 100 feet away in the lobby.
I suspect that the lack of smoke detectors in laboratories is
not unique to this building or this city. The safety office here on
campus has at least taken this matter under consideration. We also
realized that we have taken equipment reliability for granted.

Don Gantz
Boston Univ. Med. School




From: hallel :      hallel-at-macgw1.crd.ge.com
Date: 19 Apr 1996 15:18:09 U
Subject: Job Opening/GE CRD/EBSP-SEM Operator

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Message-Id: {n1382195461.81071-at-macgw1.crd.ge.com}

The Microscopy and Microanalysis Program in the Materials Characterization
Laboratory at GE Corporate Research and Development, Schenectady, NY, has an
opening for a Microanalyst/Microscopist at the Professional/Lead Professional
(BS/MS) level. The primary duties associated with this position involve the
execution of research projects involving Electron Backscatter Pattern (EBSP)
analysis of crystalline materials in the Scanning Electron Microscope (SEM).
Additional duties may involve research conducted using a variety of other
electron imaging and analysis equipment, including transmission electron
microscopes and electron microprobes. The Microscopy and Microanalysis
Program at GE CRD is one of the world's leading centers for the application of
EBSP techniques to the analysis of texture and orientation. Two dedicated
SEMs are used for this capability. The present position involves the
operation and maintenance of that equipment, and the management of project and
sample flow through the facility.

The Materials Characterization Laboratory is involved in research into the
structure and composition of materials in support of development programs both
at GE CRD and at GE businesses. A wide range of materials, including metals,
ceramics, composites, polymers, and coatings/surface modifications, are
analyzed by this group. Staff members are expected to work independently with
a high level of expertise, and to become involved with a number of major
project teams. Good communication skills, both written and oral, are
extremely important.

A BS or MS in Materials Science or a closely-related field is required. Prior
experience with electron beam instruments, particularly transmission and
scanning electron microscopes, is highly desirable. Some familiarity with
crystallography and computer usage/programming in the Windows environment is
also desired.

Resumes and other information can be sent to:

Ernest L. Hall
Manager, Microscopy and Microanalysis Program
Room K1-2C27
GE Corporate Research and Development
PO Box 8
Schenectady, NY 12301
Fax: 518-387-6972
E-mail: hallel-at-crd.ge.com





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 19 Apr 1996 17:16:45 -0400
Subject: RE- PKZIP300 virus

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Message-ID: {n1382188116.34333-at-mse.engin.umich.edu}

Subject: Time: 12:15 PM
OFFICE MEMO RE: PKZIP300 virus Date: 4/19/96

Does anyone know whether the PKZIP300 virus reported by Scott Walck is
directed at Macintosh or IBM computers?? or can it infect both??
W. C. Bigelow (bigelow-at-umich.edu)





From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 19 Apr 1996 14:20:14 -0400 (EDT)
Subject: Re: Backscattered electron imaging

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Dear Richard,

} Hello All,
} I have recently strted trying to do some quantitative backscattered
} imaging of lead zirconium titanate (PZT). I'm getting rather puzzled by the
} results and wondered if anybody had any ideas...
}
} I naively took some papers at face value which state that backscattered
} intensity is directly proportional to the mean atomic number of the material.
I just taught my radiation sciences class about nuclear backscatter-
ing (it was used as an analytical method on the moon, BTW), and the text--
Nuclear and Radiochemistry, Friedlander, et al., p430--states that the cross
section is proportional to Z^2. Since it is a Coulomb scattering, and since
e-e back scattering would have zero energy, I would expect electron back
scattering to have the same Z-dependence as proton backscattering. Per-
haps David Joy will comment.

} However, I am getting about twice the backscattered signal from PZT that I
} expect. The composition has been confirmed by microprobe analysis, so I guess
} that complex compounds and/or oxides behave differently from single elements
} and binary metals (?)

This shouldn't be the case for backscattering. There might be minor
differences for low-angle scattering due to differences in the valence elec-
tron distribution--the low-order CBED amplitudes are sensitive to this.

} I also see very strong differences in contrast between a nanocrystalline
} phase (pyrochlore) and a large grained phase (perovskite), although both are
} _supposed_ to be of the same composition. Am I actually seeing composition
} differences or does the grain size have a large effect on backscattered
} intensity?

Does the effect vary with the orientation of the large grains?

} Both effects are present when using a TEM (accelerating voltage 20-100kV)
} and a FEGSEM (accelerating voltage 0.5-25kV).

I am not surprised that you see no dependence on voltage.
Yours,
Bill Tivol




From: hallel :      hallel-at-macgw1.crd.ge.com
Date: 19 Apr 1996 15:44:31 U
Subject: Call for Papers/Fall 1996 MRS/Interface Engineering

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Message-Id: {n1382193754.78269-at-macgw1.crd.ge.com}
"Carter, Barry" {carter-at-cems.umn.edu}
X-Mailer: Mail*Link SMTP-MS 3.0.2

Call for Papers

Symposium W: Interfacial Engineering for Optimized Properties
Fall 1996 Materials Research Society Meeting
December 2-6, 1996

Description:
Interfaces often exert a controlling influence on the properties of materials.
Consequently, there is a great desire to engineer these interfaces in order
to optimize beneficial properties and eliminate those that are deleterious.
This optimization is usually achieved by fabrication or processing methods to
control the composition, structure, or crystallography of the interface. This
four day symposium will address these topics in metals, ceramics, and
composites. The discussion will primarily center on internal interfaces,
including grain boundaries, interphase interfaces, and film/coating-substrate
interfaces. The papers will include those which discuss problems which have
been addressed by interfacial engineering and those that discuss the
development of methods of interfacial engineering.

The topics to be covered, as they apply to interfaces, include the following:
o Strength and toughness
o Cohesion and adhesion
o Corrosion and embrittlement
o Fracture and fatigue
o Electrical and optical properties
o Creep and diffusion
o Reactions and mobility
o Measurement and characterization

Partial list of invited speakers : Yet-Ming Chiang (MIT); Gino Palumbo
(Ontario Hydro-electric); Fennell Evans (University of Minnesota); Michael
F. Henry (GE CRD): Vinayak Dravid (Northwestern); Ali Argon (MIT); Kathi
Alexander (ORNL)

Abstracts must be received at MRS Headquarters no later than June 21, 1996 and
must follow the standard MRS abstract model. Abstract templates and
additional information on the meeting can be obtained from MRS (E-mail:
fall96-at-abstracts.mrs.org; WWW: http://www.mrs.org; Phone: 412-367-3003;
Fax: 412-367-4373) or from the symposium organizers.

Symposium Organizers:

Clyde L. Briant
Div. of Engineering
Brown University
PO Box D
Providence, RI 02912
Phone: (401) 863-2626
Fax: (401) 863-7677
E-mail: briant-at-engin.brown.edu

C. Barry Carter
Dept. of Chem. Eng. & Mat. Sci.
Univ. of Minnesota
421 Washington Ave., SE
Minneapolis, MN 44544
Phone: (612) 625-8805
Fax: (612) 626-7246
E-mail: carter-at-cems.umn.edu

Ernest L. Hall
GE CRD
PO Box 8
Room K1-2C27
Schenectady, NY 12301
Phone: (518) 387-6677
Fax: (518) 387-6972
E-mail: hallel-at-crd.ge.com






From: paulc-at-gps.caltech.edu (Paul K. Carpenter)
Date: Fri, 19 Apr 1996 11:05:01 -0700
Subject: Re: Detection of Boron

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On the subject of Boron analysis:

} Mark Darus wrote:
}
} My EDX is a TN-5500 and I'm trying to determine if it is sensitive,
} enough to detect Boron. I have some standards, and in a moment I'm
} going to set up some boric acid and look into it. Just placing it in
} the chamber and getting the counts up, plus switching it to thin window
} doesn't give me overwhelming success from an SPI metal standard that I
} have. I'm sure there is much more to it than that. Is there any advice
} that you may have to offer. My beam current settings are 1, 5, 10, 20
} & 30 KeV.

Just a few comments about the composition of the detector window material
on EDS detectors. The mass absorption coefficients (mac) for Boron Ka
radiation by different absorbing elements are (these values from table 14.3
of Goldstein et al):

Absorber mac
H 1,723
Be 69,937
B 2,861(?)
C 5,945
N 10,118
O 15,774

So B Ka is strongly absorbed by a Be window on your EDS detector, and this
(as you know) is the reason that B Ka cannot be detected with this normal
window material. Note that carbon is fairly transparent to B Ka, either as
an EDS window material via a diamond window, or as a conductive coating
layer on your (nominally non-conductive) specimen. You should be able to
detect B Ka on a carbon-coated sample and/or with a diamond window just
fine; too thick a carbon coat will absorb just about anything, but
comparatively it is not too bad. If you have a BN window, then the
absorption is a little stronger than for the diamond window (average the
macs for B and N). Even slightly worse is for the case of an ice layer
(think of it as a layer of oxygen).

Now if you have a too thick carbon coat, and a thick layer of ice on the
detector crystal, and maybe you are using a thin Be window or a thicker BN
window, then B Ka detection is going to be difficult. As mentioned before,
reconditioning the EDS detector to get rid of the ice layer is mandatory
before attempting to do light element work like this.

Secondly, the pulse processor has to be optimized for processing low energy
x-ray pulses -- if the discriminator is set too high you are filtering out
all the low energy x-ray pulses, and if the time constant is set
incorrectly you may not be getting the best throughput on the detector.
Refer to the manuals at this point.

Suffice to say that optimization of the detector should be done on a pure
element (lump boron) before trying to look at lower levels in your samples.

It was also pointed out by Chuck Garber:
} There is just no better "standard" than a solid lump of pure boron! It
} is inert and it won't be changed by the electron beam. Boric acid will
} be unstable and as others pointed out, it will sublime causing all
} kinds of other problems.

Note however, from the mac values above, that B Ka is strongly absorbed by
many materials (for example, the mac for B Ka by Silicon is 83,702), so you
may see very strong absorption in a B-Si alloy (in fact, you may not
observe a peak at all for lower concentrations of boron because it is
absorbed entirely within the sample!). In this example, the absorption
correction via the "A" part of the ZAF correction is very high for your
sample, compared to your standard (assuming you used pure boron). The
bottom line here is that you absolutely *must* use a standard that is as
close as possible to your samples for good quantitative correction. Of
course, the available boron standards are pure boron, boron nitride (which
is typically polycrystalline and is not a good conductor; single crystal BN
is much better but hard to find in pieces larger than a few microns), and
numerous borosilicate glasses (which of course have the problem of
absorption by silicon). For EDS work you probably have to use pure boron
to avoid peak overlaps, but for WDS analysis things like BN can be used.

The magnitude of the absorption correction is directly related to the
accelerating voltage used, so if you can do analysis at 10 KV rather than
15 KV, you are better off. You can work at lower voltages (like your 5 KV
setting), but the sample surface must be really clean, and you then get
into problems where aspects of the correction algorithms are not really
valid for this range of accelerating voltages.

As a final note, it is relatively meaningless to look at EDS spectra of
pure elements when shopping for an EDS system to do light element analysis.
It is the performance on typical multi-element samples that really
matters. For example, I was looking at the possibility of studying
synthetic vs. natural emeralds (Be3Al2Si6O18) including inspection of the
Be Ka peak by EDS. Even with a freshly reconditioned detector on *open
window* mode, with the SEM probe current full up, no Be peak was observed.
So Be measurement was just not meant to be on an EDS-SEM system. It turns
out that even by WDS, using a Mo4C analyzing crystal (red-hot for Be and
B), and the probe current full up on our microprobe (400 nA -at- 10KV), and
analyzing at low accelerating voltage, and a clean high vacuum turbo-pumped
system, that Be in emerald is barely detectable (count times of up to an
*hour* were used!). I'll just say that boron analysis is a walk in the
park compared to beryllium analysis...

Anyway, good luck!

Paul Carpenter


+----------------------------------------------------+
| Paul K. Carpenter paulc-at-gps.caltech.edu |
| Division Analytical Facility |
| Geological and Planetary Sciences MC 170-25 |
| California Institute of Technology |
| Pasadena, CA 91125 |
| 818-395-6126 (X-ray Lab) 818-568-0935 (Dept. FAX) |
+----------------------------------------------------+






From: K1JIA-at-VAXC.STEVENS-TECH.EDU
Date: Wed, 17 Apr 1996 20:03:39 -0500 (EST)
Subject: UNSUBSCRIBE

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From: MikGu-at-mme.liu.se (Mikael Gustafsson)
Date: Sun, 21 Apr 1996 11:52:07 +0200
Subject: Hose

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I would like to know if any one has investigated gas permeability properties
of plastic/rubber hose. I'm trying to set up a microscope perfusion system
with flow from a solution with CO2 at a specific concentration. The problem
is that the silicon rubber hose used for interconnection between the parts,
cannot keep CO2, which very quickly escapes into surrounding air.

Has anyone heard of a gas impermeable hose (inner diameter ca 1 mm or less) or


Best regards


=============================================
Mikael Gustafsson MD, PhD
Dept Med. Microbiology and
Dept Internal Medicine, Cardiology section
University Hospital of Linkoping
S 581 85 LINKOPING
SWEDEN

E-Mail: MikGu-at-mme.liu.se
FAX: 046/13/224789
Phone: 046/13/224783
=============================================





From: Brett W. Cockman :      bcockman-at-uniwa.uwa.edu.au
Date: Mon, 22 Apr 1996 09:29:16 +0700 (WAST)
Subject: RE: Semi-thin sections.

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Randi,
I suggest transferring your semithin sections to a water bath at about 50
Cand pick up on acid clean slides (this is most important!). Dry on a hot
plate and then place in hot oven (about 60 C +) for at least an hour. This
will guarantee that your sections stay on the slide. I further suggest you
remove the resin by treatment with potassium ethoxide (saturated KOH in
ethanol; make up at least 24 hours before use then filter):
1. Treat with K-ethoxide for about 3 - 4 mins.
2. Wash well with 3 changes of absolute ethanol.
3. Hydrate through 95%, 70% to distilled water and then dry.
4. Stain with toluidine blue or any other stain!

If you need more details please ask.

.
.
Brett W. Cockman
Technologist in Charge
School of Dentistry
University of Western Australia
Voice: (619-2205834)
Fax: (619-2213829)
e-mail; bcockman-at-uniwa.uwa.edu.au




From: Donna Wagahoff :      DWAGAHOF-at-wpsmtp.siumed.edu
Date: Mon, 22 Apr 1996 10:30:33 -0600
Subject: venting print processors

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We just purchased a new Mohr-Pro print processor but have not been
able to use it because our building engineer is concerned that we must
vent the unit. The manual does not address this issue and there is no
outlet or attachment for venting the chemistry fumes. We have had
several processors over the years, with no exhaust vent and have had
no problems. Would you please share with us whether you have your
print processors connected to a vent system, how you accomplished it,
if it is an OSHA requirement, if your system came with an outlet made
specifically for venting chemistry fumes and any other pertinent
information. Thanks for your assistance.

Donna Wagahoff
SIU School of Medicine
Springfield, Il.
217-782-0898
fax 217-524-3227





From: moxtek-at-MOXTEK.WIN.NET (Clark Turner)
Date: Mon, 22 Apr 1996 09:13:08
Subject: Re: Boron analysis

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X-Mailer: WinNET Mail, v2.51
Message-ID: {67-at-moxtek.win.net}
Reply-To: moxtek-at-MOXTEK.WIN.NET (Clark Turner)
To: darus-at-cle.dnet.ge.com, microscopy-at-Sparc5.Microscopy.Com

Mark,

A good sample to test the detector capability is solid boron
nitride. It not only gives you the boron K-alpha peak, but also
the nitrogen K-alpha for reference.

D. Clark Turner
Director, Thin Film Products Group
MOXTEK, Inc.
452 West 1260 North
Orem, Utah 84057
phone (801) 225-0930
email moxtek-at-moxtek.win.net



}
}
}
} Hello,
} My EDX is a TN-5500 and I'm trying to determine if it is sensitive,
} enough to detect Boron. I have some standards, and in a moment I'm going to
} set up some boric acid and look into it. Just placing it in the chamber and
} getting the counts up, plus switching it to thin window doesn't give me
} overwhelming success from an SPI metal standard that I have.
} I'm sure there is much more to it than that. Is there any advice that you may
} have to offer. My beam current settings are 1, 5, 10, 20 & 30 KeV.
}
} Mark Darus
}
} Darus-at-cle.dnet.ge.com
}





From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 22 Apr 1996 09:51:49 -0700 (PDT)
Subject: Re: Semi-thin sections.

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Randi,

As already suggested, cleaning the slides is important. For different
plastics, soaking slides for 30 min. in 50% bleach (commercial bleach
mixed 1:1 with dstilled water), or in 70% ethanol containing 1% HCl, will
do the trick instead of messing about with chromic acid. Wash several
times with destilled water after cleaning.

What are you coverslipping with? Some mountants cause wrinkling unless
slides are very clean or the mountant is cut 1:1 with xylene.

With the above approaches, we get reliably flat sections and etch only
when necessary for immunocytochemistry.



Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu


On Sat, 20 Apr 1996, Randi Olsen wrote:

} Hello,
} I wonder if there is any help somewhere out there:
} Lately we have been fighting some catfish larvaes, embedded
} in Epon/Araldite. Because we need sections of the whole
} length og larvaes up to 4 weeks old, we need big semithin
} sections, and they dont want to strech properly. I have
} small foldes (or waves) in the sections wisible at x40 that
} make it impossible to get sharp photographs at this
} magnification. (The foldes also trap some air between the
} glass slide and the sectiokn). Is there anything we can do
} to make the sections flatter?
}
} Thanks in advance.
}
} Randi Olsen
} Department of Electron Microscopy
} University of Troms=F6
} Norway
}
}
}





From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 22 Apr 96 14:55:49 EDT
Subject: Backside Emission Microscopes?

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I am trying to collect information from the various manufacturers of Backside
Emission Microscopes. If anyone can provide me with names and phone numbers of
appropriate manufacturers, I would greatly appreciate it.

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
sbt-at-msa.microscopy.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 22 Apr 96 14:57:29 EDT
Subject: ASTM Home Page?

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Can anyone provide me with the URL for the ASTM home page?

Thank you!

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
sbt-at-msa.microscopy.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Mon, 22 Apr 1996 16:00:41 -0500 (EST)
Subject: Re: venting print processors

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Dear Donna,

Why does the engineer think the processor needs to be vented? There is very
very little mixture of chemicals (ie ammonia fumes). If he/she is so concerned
let them design and make a vent for it. The least that should be done is to
make sure there is enough room ventalation. I myself you not worry.

Best of Luck,
Ed Calomeni
Medical College of Ohio
Toledo, OH 43699
emlab-at-opus.mco.edu




From: EmLab
Date: 4/22/96 2:31 PM
Subject: Re: venting print processor

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"microscopy-at-Sparc5.Microscopy.Co" {microscopy-at-Sparc5.Microscopy.Com}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Reply to: RE} } venting print processors

Donna,

Our safety people required us to have an emergency exhaust fan (with a manual
switch) installed in the darkroom in which the processor is located. Other
than occasional testing, the fan has not been operated in the ten years since
it was installed.

Mike O'Keefe
National Center for Electron Microscopy
Berkeley, CA 94720
--------------------------------------

Why does the engineer think the processor needs to be vented? There is very
very little mixture of chemicals (ie ammonia fumes). If he/she is so
concerned let them design and make a vent for it. The least that should be
done is to make sure there is enough room ventalation. I myself you not
worry.

Best of Luck,
Ed Calomeni
Medical College of Ohio
Toledo, OH 43699
emlab-at-opus.mco.edu






From: slc6-at-lehigh.edu (Sharon Coe)
Date: Tue, 23 Apr 1996 15:40:41 -0400
Subject: Biological SEM

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Please post the following on the Microscopy Server:

Biological SEM and X-ray Microanalysis

The 1996 Lehigh Short Course, in addition to teaching the theory and basic
prractical skills associated with scanning electron microscopy and x-ray
microanalysis, also provides a thorough understanding of how specimens
should be prepared for imaging and analysis.

Special emphasis is placed on providing answers to the problems associated
with the preparation, microscopy, and analysis of biological, organic,
hydrated and bio-medical materials such as plastics, paints, polymers,
elastomers, insulators, cements, resins, lubricants, pharmaceutical agents,
foods and dairy products, textiles, paper, archeological remains and the
whole spectrum of plant, animal and human tissues. The very nature of
these samples, which are generally beam-sensitive, frequently wet, and
invariably hetereogeneous, require an appreciation and understanding of a
wide range of new techniques and methods. The Lehigh Short Course is
uniquely placed to provide the necessary information and hands-on
instrumentation to address these problems.

Specific instruction will be given in low temperature and low voltage
microscopy and analysis; sample preparation and specimen coating; immuno-
and histo-chemical and staining techniques to localize specific chemical
ligands; non-invasive preparative methods and techniques for diffusible and
soluble substances; environmental microscopy and analysis, and the
strategies and tactics to employ when examining and analysing
beam-sensitive samples. Special biological lectures by Dr. Patrick Echlin,
University of Cambridge.

For a free brochure contact:

Sharon Coe
Materials Science Department
Lehigh University
5 East Packer Avenue
Bethlehem, PA 18015
Phone: 610/758-5133
Fax: 610/758-4244
e-mail: slc6-at-lehigh.edu






From: IAN HALLETT :      ihallett-at-MARCCRI.MARC.CRI.NZ
Date: Tue, 23 Apr 1996 09:00:32 GMT+1200
Subject: Re: venting print processors

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Message-Id: {m0uBVpX-0003L7C-at-fast.net}

Dear Donna

We have an externally venting overbench extraction hood over both
the print processor and an adjacent wet area. This appears to work
well and certainly eliminates the smell of the warm chemicals.

All photographic processing should be carried out with some form of
fume extraction, something that is often ignored. Our photographic
section installed such hoods in their darkrooms 12 or 13 years ago
after one of the photographers developed respiratory problems
carrying out conventional hand printing..

Ian Hallett

(Donna Wagahoff writes ...)
} We just purchased a new Mohr-Pro print processor but have not been
} able to use it because our building engineer is concerned that we must
} vent the unit. The manual does not address this issue and there is no
} outlet or attachment for venting the chemistry fumes. We have had
} several processors over the years, with no exhaust vent and have had
} no problems. Would you please share with us whether you have your
} print processors connected to a vent system, how you accomplished it,
} if it is an OSHA requirement, if your system came with an outlet made
} specifically for venting chemistry fumes and any other pertinent
} information. Thanks for your assistance.
}
} Donna Wagahoff
} SIU School of Medicine
} Springfield, Il.
} 217-782-0898
} fax 217-524-3227

**************************************************


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660
EMail ihallett-at-hort.cri.nz




From: Brett W. Cockman :      bcockman-at-uniwa.uwa.edu.au
Date: Tue, 23 Apr 1996 10:42:21 +0700 (WAST)
Subject: RE: Semi-thin sections.

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X-NUPop-Charset: English

Hello Robert,
In answer to your questions:
1. I usually use a cotton bud to pick up the sections and 'roll' them onto
the surface of the waterbath.
2. I havn't used spurr's resin or mixtures with other epoxy's but it is
worth a try!.
3. I add KOH ot cover the base of a 1lt reagent bottle (screw top) and fill
with ethanol (absolute). The solution goes brown after a day or so , then
filter before use. It keeps reasonably well. Please note that it is very
corrosive!!!.
4. The original reference is: Imai, Y et al (1968): Removing method of resin
for histological section following halogenation. Electron Microsc. vol 17,84

Regards,

Brett.




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Mon, 22 Apr 1996 17:16:20 -0600
Subject: Central States Microscopy Meeting

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******************************************************************************

ENJOY MICROSCOPY AND THE GREAT OUTDOORS - WHAT A COMBINATION!

******************************************************************************

The Spring meeting of the Central States Microscopy Society will take place
on June 21, 1996 in Carbondale, IL at the Giant City State Park Lodge. The
theme of the meeting will be "Ride the Wave" (e.g., technological wave).
Innovative uses of new and established technologies (microwave, LM, SEM,
TEM, scanned probes, etc) are invited. Biological and physical science
presentations are welcome.

Cash prizes for best student presentations. Typically this ranges from
$50-100, depending upon number of entrants and quality of work presented.
Abstracts must be received 3 weeks prior to meeting to be considered in the
student competition. Follow MSA abstract guidelines.

CSMS corporate sponsors are invited to participate by means of talks and
demonstrations of equipment. Contact me well in advance of the meeting,
however, if you require tables, space or special electrical setups. There
will be no charge for CSMS corporate sponsors.

Programs, lodging recommendations, directions to meeting site will be sent
several weeks in advance of the meeting.

Speakers and presentors: please contact John Bozzola for topics/timing no
later than May 21, 1996.


******************************************************************************






#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Tue, 23 Apr 1996 17:57:23 +1100
Subject: Re: venting print processors

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We have a Rapiline print processor which came with a (very small) built-in
fan to extract fumes from the tanks... and blow them into the room. The
installation did not include connecting this to any extract system although
the technician indicated that this could be done. Unlike your experience
however, we found the fumes unpleasant so we had our workshop make elbows
and ducts to connect the output to the building extract sustem and we have
noticed that the air is much fresher in the darkroom as a result. I don't
know if the fumes were unsafe before but it is certainly more pleasant to
use be in that room now and we feel that it was a worthwhile modification.
I suppose whether or not is worthwhile depends a lot what photographic
chemistry you are using and at what temperature (we use 27degC which is
relatively cool). I wouldn't assume that because the manual doesn't mention
it that it isn't worth doing, my expectation of manuals gets lower with
every new bit of equipment we buy.

Best regards,

Richard


Richard Easingwood
South Campus Electron Microscope Unit
School of Medical Sciences
University of Otago
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD















From: Bo Johansen :      BOJ-at-bot.ku.dk
Date: Tue, 23 Apr 1996 08:11:24 GMT+0200
Subject: Mounting medium

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Hello fellow microscopists

I need a little help.
I am using the NBT/BCIP method to detect alkaline phosphatase in
indirect immunomarking on plant tissue sections. I want to
mount these sections permanently and I have tried DPX but the reduced
tetrazolium salt just (re)crystallize and any specific staining is
lost. Any suggestion about what to use instead of DPX is much
appreciated.

Thank you

Bo

_____________________________________________________________________
Bo Johansen E-Mail: BoJ-at-bot.ku.dk
Botanical Institute Vioce: +45 3532 2157
Gothersgade 140 FAX: +45 3313 9104
DK-1123 Copenhagen K, Denmark http://www.bot.ku.dk/www/staff/boj.htm
---------------------------------------------------------------------






From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Tue, 23 Apr 1996 08:43:50 -0500
Subject: Independent Sales Representatives

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Nanoprobes, Inc is looking for independent sales representatives to carry their
immunoreagent product line. This position(s) are commission only. Please send
inquires/resumes to Ms. Tempel at Nanoprobes, Inc. 25 E. Loop Rd. Ste 124,
Stony Brook NY 11790-3350,Phone: 516 444 8815 or Fax 516 444 8816;
E.mail: nano-at-mail.lihti.org.








From: Mev H van der Merwe :      HVDM-at-op1.up.ac.za
Date: Tue, 23 Apr 1996 15:04:29 GMT+2
Subject: unsubscribe

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please unsubscribe




From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Tue, 23 Apr 1996 10:42:13 -0500
Subject: Independent Sales Representatives

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Message-Id: {199604231440.KAA25783-at-lihti.org}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Nanoprobes, Inc is looking for independent sales representatives to carry their
immunoreagent product line. This position(s) are commission only. Please send
inquires/resumes to Ms. Tempel at Nanoprobes, Inc. 25 E. Loop Rd. Ste 124,
Stony Brook NY 11790-3350,Phone: 516 444 8815 or Fax 516 444 8816;
E.mail: nano-at-mail.lihti.org.











From: olinsal-at-bio.ornl.gov (Ada L. Olins)
Date: Tue, 23 Apr 1996 10:41:49 +0500
Subject: RE: Semi-thin sections.

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I have good success picking up semi-thin sections with a fine wire loop
that I attached to a wooden stick. With a high meniscus approach the
section from below and lift up. The section can then be deposited on a
water droplet (on a slide) either by inverting the loop on the water
droplet or by floating the section off. Heat slide GENTLY to avoid
wrinkles and dry the section.

For em: I usually transfer the section to a beam capsul filled with
water, and put it in a warm oven (at 37-45 deg.C), to remove the wrinkles.
I then use a plastic film-(e.g. parloidin) coated grid to pick up the
section. This prevents "electrostatic jumping" of the section when it is
dry.

Good luck, Ada

ADA L. OLINS
THE UNIVERSITY OF TENNESSEE-OAK RIDGE and
THE BIOLOGY DIVISION, ORNL
P.O. BOX 2009 TEL: 423 574 1269
OAK RIDGE, TN 37831-8077 FAX: 423 574 1274








From: ENERGY BEAM SCIENCES, INC :      75767.640-at-CompuServe.COM
Date: 23 Apr 96 10:35:37 EDT
Subject: Re: manuals

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Richard Easingwood wrote something in his recent post regarding ventilation and
film processors that struck a raw nerve. He wrote:
"I wouldn't assume that because the manual doesn't mention it that it isn't
worth doing, my expectation of manuals gets lower with every new bit of
equipment we buy."

We have just received CE (European Community) certification for our laboratory
microwave processor after many months and many thousands of dollars. CE
compliance with electrical safety regulations has been required since Jan 1,
1996, for *all* electrical equipment exported into Europe.

Many of the CE regulations relate to what must be in the users' manual.
Everything that is required for the manual must be translated into several
languages, in addition to conforming to complicated rules regarding content and
format. The end result is that we've cut our very thorough manual of almost 200
pages down to fewer than 20 pages at the urging of our CE certification
consultant.

I would expect that the trend that Richard points out in his post will continue
to worsen as more international manufacturing companies deal with the new CE
regulations.

Steven Slap, Vice-President
ebs-at-ebsciences.com





From: BARBARA.HARTMAN-at-smtpgw.inet.spri.sp.com (BARBARA HARTMAN)
Date: Tue, 23 Apr 1996 14:36:02 -0400
Subject: General Announcement

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Protocols in:

Microscopic Imaging, Immunocytochemistry and Image Analysis

Twenty-Second Annual Program

The George Washington University Center for Microscopy
and Image Analysis

June 4-7, 1996

For further course information, please contact:

Mr. Fred Lightfoot
202-994-2881 (PHONE)
202-994-8885 (FAX)
E-Mail: FredL-at-INDY.CMIA.GWUMC.EDU




From: MELLIOTT-at-prl.pulmonary.ubc.ca
Date: Tue, 23 Apr 1996 12:12:22 +0800PST
Subject: anti-myeloperoxidase

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Does anyone know of a source for antibodies to rabbit myeloperoxidase??
Can't find them in Linscott's or the MSRS catalogue. Do any of the
antibodies to human cross-react with rabbit??

Thanks
Mark Elliott
Pulmonary Research Lab, UBC
Vancouver Canada





From: cal-at-ssnet.com (cal Montgomery)
Date: Tue, 23 Apr 1996 16:00:11 -0400
Subject: Lehigh course

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Have a customer interested in EM courses. I suggested the Lehigh course.
Would like to know who to have her contact to get info on the Lehigh course.
TIA
Cal Montgomery





From: Carmine M. Pariante :      cparian-at-emory.edu
Date: Tue, 23 Apr 1996 17:30:13 -0400 (EDT)
Subject: Lehigh course

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please unsuscribe





From: Gary H. Zajic :      zajic-at-umich.edu
Date: Tue, 23 Apr 1996 15:16:17 -0400 (EDT)
Subject: blocking endogenous peroxidase

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Hello, I am doing immunocytochemical experiments on mouse cochlea
using frozen sections. I find that I must block endogenous peroxidase
in these tissues. Most protocols I see require dehydration to 100%
EtOH or MeOH when blocking with H2O2. Cochlear tissue is very delicate
and there is a risk losing some of the fine structure with these
additional steps. My questions are: why can't I just block with H2O2
in my buffer? is the alcohol necessary for penetration allowing better
access of the H2O2 to the tissue? These are 10 micron sections.
Thanks in advance. Sincerely, Gary


zajic-at-umich.edu




From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 4/22/96 1:56 PM
Subject: TEM Contract Lab

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Anyone out there know of a lab where we can get some TEM work done near the
Irvine, California
area. Within Orange County will do. Thanks to all.





From: Louisa.Howard-at-Dartmouth.EDU (Louisa Howard)
Date: 23 Apr 96 13:19:05 EDT
Subject: Long term fixation

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Message-id: {13739592-at-prancer.Dartmouth.EDU}

Our lab has been asked to help on a possible project studying mammalian brain
tissue. The material will be collected in the field( a few thousand miles fom
our lab) and storage times could be up to 3-4 weeks, before the material
reaches the lab for processing. I have an excellent fixation method for brain
tissue, but its fairly involved and the solutions must be made up fresh; not a
real possibility for this field work. In the past, I have had great success,
using PIPES buffer for the glutaraldehyde fixation, whenever mammalian em
material had to be stored for 3-4 days at 4 degrees C. I would make up the
fixative bottle of 4% GTA in 0.15M PIPES, pH 7.35 and send it out. They would
add their samples and mail(at -at-4 degrees C) them to me. I've also had suceess
using the GTA/PIPES fixation and then transferring samples to a buffer of 0.1M
Na Cacodylate with 0.15M Sucrose, pH 7.4. and holding the sample for a week,
at 4 degrees C.
Has anyone had experience in keeping a sample for 4 weeks before final
processing. Would it be better to store the sample:
1. in PIPES buffer, after GTA/PIPES fixation?
2. 0.1M Na Cacodylate with 0.15M Sucrose, pH 7.4. after GTA/PIPES fixation?
3. leave in GTA/PIPES fixative for the entire time at 4 Degrees C. ( I worry
about the staibility of the GTA over such a long time)
4. Some other method, that works with mammalian tissue.

Any suggestions or thoughts on long term fixation/storage would be greatly
appreciated.
thanks
Louisa Howard




From: becks-at-sunynassau.edu (Steve Beck)
Date: Tue, 23 Apr 1996 19:20:34 -0500
Subject: Summer TEM Course Announcement

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SUMMER COURSE ANNOUNCEMENT - Transmission Electron Microscopy (BIO. 221)

NASSAU COMMUNITY COLLEGE

A five week course in Biological Transmission Electron Microscopy is being
offered by the Biology Department of Nassau Community College. This is a 4
credit course offered over the first summer session, between May 28 and
June 27, 1996. The class meets from 8:00 am to Noon four days per week
(usually Monday thru Thursday).

This is a "hands-on" course emphasizing biological specimen preparation,
ultra-thin sectioning involving block trimming, glass knifemaking and
operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III),
thick and ultra-thin section mounting and contrast staining (UA and Pb
citrate), grid support films (formvar, carbon), student operation of the
TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs
through the process of black & white photography, and electron micrograph
analysis. Students will work on a chosen sample(s) with the goal of
producing a portfolio of high quality TEM photomicrographs of that
sample(s).

The course is widely transferrable and the cost per credit is reasonable at
$78 per credit.

Registration for summer courses can be conducted via phone by calling (516)
572-7131 or (516) 572-7372 or (516) 572-7425, Monday through Thursday
2:30pm to 7:00pm (This phone service ends on May 2, 1996)

More information about summer registration and course offerings is
available at our web site {http://www.sunynassau.edu}

The catalog description is specified below. If you have further questions,
you should e-mail me directly at the address below.

CATALOG DESCRIPTION
BIO 221: Transmission Electron Microscopy 4 cr.
Prerequisites: BIO 109-110 or equivalent, CHE 151-152 or equivalent.
An introduction to the basic principles of transmission electron
microscopy including tissue preparation, microscope (TEM) operation, black
& white photography, and micrograph interpretation. The entire laboratory
is devoted to the development of skills and preparative techniques involved
with the operation of an actual transmission electron microscope.
(3 lecture, 3 laboratory hours). Laboratory fee applies.



Stephen Beck
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829





From: knoff-at-lipovx.lbl.gov (Laura Knoff)
Date: Tue, 23 Apr 1996 12:37:01 -0800
Subject: No. Cal Micro. Meeting

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} Northern CA Society for Microscopy (NCSM) Meeting Announcement
}
} Where: Roche Biochemicals (formerly Syntex), Gallery
} Conference Center Bldg A-2
} 3401 Hillview Ave, Palo Alto CA
}
} When: Thursday, May 9, 1994, from 2:30 to 8:45 PM
}
}
} 2:30 -3:00 Registration
} Speakers
} 3:00-3:45 Hank W. Bass, UC Berkeley, Molecular and Cellular
} Biology, "Analysis of Meiosis using 3-D
} Deconvolution Light Microscopy". 4:00-4:45 David Blake,
} NASA Ames Research Center, Space Sciences Division
} "Electron Microscopy of Astrophysical Ice"
} 5:00 - 6:30 Social Hour and Local Society A filiate meeting
} sponsored by JEOL Instruments
} 6:30-7:30 Dinner. Choices: Marinated Stripped Loin with sundried
} tomatoes, Lemon chicken with caper sauce, or roasted
} vegetable lasagna,
} each with garlic mashed potatoes, summer squash medley,
} mixed green salad, dinner rolls, and dessert.
} 7:45 - 8:45 Paul Carpenter, Manager Analytical and Geology, Cal
} Institute of Technology, "XRay and Optical
} Methods as Applied to the Study of Gemstones."
}
} Please help us to plan accurately. Make your reservation before Friday MAY
} 3rd
} Fax reservation(s) with meal preference to Laura Knoff at (510) 486-4750.
} E-Mail address = KNOFF-at-LIPOVX.LBL.GOV
} Phone = (510) 486-4088, if leaving a message please speak slowly and clearly.
} Price: $17 for Loin, $14 for Chicken, $13 for Lasagna,$8 for student
} members.
} Avoid standing in line by pre-paying. Send your check, payable to NCSM,
} to NCSM c/o Laura Knoff
} LawrenceBerkeley National Lab
} Bldg 1, Room 264
} 1 Cyclotron Rd
} Berkeley, CA 94720
Sorry about the format (or lack of it).






From: knoff-at-lipovx.lbl.gov (Laura Knoff)
Date: Tue, 23 Apr 1996 12:41:58 -0800
Subject: No. Cal Micro. Meeting

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} Date: Tue, 23 Apr 1996 12:37:02 -0800
} To: microscopy-at-Sparc5.Microscopy.Com
} From: knoff-at-lipovx.lbl.gov (Laura Knoff)
} Subject: No. Cal Micro. Meeting
} Cc:
} Bcc:
} X-Attachments:
}
} } Northern CA Society for Microscopy (NCSM) Meeting Announcement
} }
} } Where: Roche Biochemicals (formerly Syntex), Gallery
} } Conference Center Bldg A-2
} } 3401 Hillview Ave, Palo Alto CA
} }
} } When: Thursday, May 9, 1994, from 2:30 to 8:45 PM
} }
} }
} } 2:30 -3:00 Registration
} } Speakers
} } 3:00-3:45 Hank W. Bass, UC Berkeley, Molecular and Cellular
} } Biology, "Analysis of Meiosis using 3-D
} } Deconvolution Light Microscopy". 4:00-4:45 David
} } Blake, NASA Ames Research Center, Space Sciences Division
} } "Electron Microscopy of Astrophysical Ice"
} } 5:00 - 6:30 Social Hour and Local Society A filiate meeting
} } sponsored by JEOL Instruments
} } 6:30-7:30 Dinner. Choices: Marinated Stripped Loin with
} } sundried tomatoes, Lemon chicken with caper sauce, or
} } roasted vegetable lasagna,
} } each with garlic mashed potatoes, summer squash
} } medley, mixed green salad, dinner rolls, and
} } dessert.
} } 7:45 - 8:45 Paul Carpenter, Manager Analytical and Geology, Cal
} } Institute of Technology, "XRay and Optical
} } Methods as Applied to the Study of Gemstones."
} }
} } Please help us to plan accurately. Make your reservation before Friday MAY
} } 3rd
} } Fax reservation(s) with meal preference to Laura Knoff at (510) 486-4750.
} } E-Mail address = KNOFF-at-LIPOVX.LBL.GOV
} } Phone = (510) 486-4088, if leaving a message please speak slowly and clearly.
} } Price: $17 for Loin, $14 for Chicken, $13 for Lasagna,$8 for student
} } members.
} } Avoid standing in line by pre-paying. Send your check, payable to NCSM,
} } to NCSM c/o Laura Knoff
} } LawrenceBerkeley National Lab
} } Bldg 1, Room 264
} } 1 Cyclotron Rd
} } Berkeley, CA 94720
} Sorry about the format (or lack of it).
}






From: knoff-at-lipovx.lbl.gov (Laura Knoff)
Date: Tue, 23 Apr 1996 12:41:58 -0800
Subject: No. Cal Micro. Meeting

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} Date: Tue, 23 Apr 1996 12:37:02 -0800
} To: microscopy-at-Sparc5.Microscopy.Com
} From: knoff-at-lipovx.lbl.gov (Laura Knoff)
} Subject: No. Cal Micro. Meeting
} Cc:
} Bcc:
} X-Attachments:
}
} } Northern CA Society for Microscopy (NCSM) Meeting Announcement
} }
} } Where: Roche Biochemicals (formerly Syntex), Gallery
} } Conference Center Bldg A-2
} } 3401 Hillview Ave, Palo Alto CA
} }
} } When: Thursday, May 9, 1994, from 2:30 to 8:45 PM
} }
} }
} } 2:30 -3:00 Registration
} } Speakers
} } 3:00-3:45 Hank W. Bass, UC Berkeley, Molecular and Cellular
} } Biology, "Analysis of Meiosis using 3-D
} } Deconvolution Light Microscopy". 4:00-4:45 David
} } Blake, NASA Ames Research Center, Space Sciences Division
} } "Electron Microscopy of Astrophysical Ice"
} } 5:00 - 6:30 Social Hour and Local Society A filiate meeting
} } sponsored by JEOL Instruments
} } 6:30-7:30 Dinner. Choices: Marinated Stripped Loin with
} } sundried tomatoes, Lemon chicken with caper sauce, or
} } roasted vegetable lasagna,
} } each with garlic mashed potatoes, summer squash
} } medley, mixed green salad, dinner rolls, and
} } dessert.
} } 7:45 - 8:45 Paul Carpenter, Manager Analytical and Geology, Cal
} } Institute of Technology, "XRay and Optical
} } Methods as Applied to the Study of Gemstones."
} }
} } Please help us to plan accurately. Make your reservation before Friday MAY
} } 3rd
} } Fax reservation(s) with meal preference to Laura Knoff at (510) 486-4750.
} } E-Mail address = KNOFF-at-LIPOVX.LBL.GOV
} } Phone = (510) 486-4088, if leaving a message please speak slowly and clearly.
} } Price: $17 for Loin, $14 for Chicken, $13 for Lasagna,$8 for student
} } members.
} } Avoid standing in line by pre-paying. Send your check, payable to NCSM,
} } to NCSM c/o Laura Knoff
} } LawrenceBerkeley National Lab
} } Bldg 1, Room 264
} } 1 Cyclotron Rd
} } Berkeley, CA 94720
} Sorry about the format (or lack of it).
}






From: Greg2NJ-at-aol.com
Date: Tue, 23 Apr 1996 21:02:15 -0400
Subject: Kidney Fixation for Routine TEM

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Greetings:

Am interested in finding out the best overall fixation for Kidney (medulla
and cortex).
Interested in good preservation (who isnt) as well as defined membrane
boundries. Specifically looking at mitochondria.






From: p&s-at-ultra.net.au (Probing & Structure)
Date: Wed, 24 Apr 1996 13:03:13 +1000
Subject: Home Page Probing & Structure

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Microscopists & Microanalyst:

Probing & Structure has a large fully illustrated microscopy
catalogue on the
www. Prices are shown in Australian dollars. Most items are shipped FIS.
Currency converter, numerous good links and many MSDS documents are also
available. It's worth a bookmark

http://www.ultra.net.au/~pns/index.html

Jim Darley





From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Wed, 24 Apr 1996 14:38:39 +1100
Subject: Re: Long term fixation

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}
} Any suggestions or thoughts on long term fixation/storage would be greatly
} appreciated.
} thanks
} Louisa Howard

I have had no problems with long term (years) storage of retina in
cacodylate buffer (no additives) after glutaraldehyde fixation. Long term
(weeks) storage in glut/cacodylate is also not a problem - the tissue
doesn't harden excessively - though I've never done it with brain. All at 4
deg C of course.

Diana van Driel
Dept Ophthalmology
Sydney University
AUSTRALIA 2006






From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Wed, 24 Apr 1996 07:53:02 +0100 (BST)
Subject: Re: Lehigh course

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In response to Cal Montgomery and any other people interested in EM (SEM)
courses they should contact Sharon Coe tat e-mail address
{slc6-at-Lehigh.EDU} who will be able to give you details of the all
singing, all dancing, bells and whistles LEHIGH SHORT COURSE which will
covers everything you want to know and were afraid to ask about SEM and
x-ray microanalysis of every conceivable type of specimen (as can be seen
it does not, alas, cover typing)

Patrick Echlin
Cambridge UKOn Tue, 23 Apr 1996, cal Montgomery wrote:

} Have a customer interested in EM courses. I suggested the Lehigh course.
} Would like to know who to have her contact to get info on the Lehigh course.
} TIA
} Cal Montgomery
}
}




From: Tony Bruton :      bruton-at-emu.unp.ac.za
Date: Wed, 24 Apr 1996 09:56:27 +0200
Subject: Standards for EDX on TEM

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X-Mailer: Novell GroupWise 4.1

If anyone out there has got advice/recommendations/experience on the
preparation and use of standards for TEM+EDX we would be most
interested to hear from you.

Though our emphasis will ultimately be on biological applications, any
input would be of interest at this stage.

Tony Bruton
Centre for Electron Microscopy
University of Natal, Pietermaritzburg
Private Bag X01, Scottsville 3209
KwaZulu Natal, South Africa

e-mail: bruton-at-emu.unp.ac.za





From: JSmiley-at-nwu.edu (John Smiley)
Date: Wed, 24 Apr 1996 08:42:19 -0600
Subject: Re: blocking endogenous peroxidase

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For postmortem human brain tissue, which is also delicate, we use 1% H2O2
in PBS for 1 hour at 4C. This incubation is done without shaking, because
the peroxide is also somewhat harsh on the tissue.

} }
} } Hello, I am doing immunocytochemical experiments on mouse cochlea
} } using frozen sections. I find that I must block endogenous peroxidase
} } in these tissues. Most protocols I see require dehydration to 100%
} } EtOH or MeOH when blocking with H2O2. Cochlear tissue is very delicate
} } and there is a risk losing some of the fine structure with these
} } additional steps. My questions are: why can't I just block with H2O2
} } in my buffer? is the alcohol necessary for penetration allowing better
} } access of the H2O2 to the tissue? These are 10 micron sections.
} } Thanks in advance. Sincerely, Gary
} }
} }
} } zajic-at-umich.edu






From: Walt Bobrowski (313) 996-7814 :      bobroww-at-aa.wl.com
Date: Wed, 24 Apr 1996 09:32:29 -0400 (EDT)
Subject: Re: Kidney Fixation for Routine TEM

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Retrograde perfusion fixation via abdominal aorta. See: Griffith, LD et al.
(1967). The ultrastructure of the functioning kidney. Lab. Invest. 16,
220-246.
Best regards,

Walter F. Bobrowski
Subcellular Pathology
Parke-Davis Pharmaceutical Research
Ann Arbor, MI 48105

TEL: 313-996-7814
FAX: 313-996-5001
E-Mail: BOBROWW-at-AA.WL.COM
} Greetings:
}
} Am interested in finding out the best overall fixation for Kidney (medulla
} and cortex).
} Interested in good preservation (who isnt) as well as defined membrane
} boundries. Specifically looking at mitochondria.
}






From: rosemary-at-aec.env.gov.ab.ca (Rosemary K. HARRIS)
Date: Wed, 24 Apr 1996 08:59:50 -0600
Subject: long term storage

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To expand upon Louisa Howard's question of long term storage -she is
asking about storage in cacodylate buffer.

The question has recently been asked here about long term storage in
phosphate buffers. We have a variety of fish, rat and cow tissues that were
collected (or will be collected) "just in case" EM may be needed. Some of
these tissues have been around for at least 10 years and will likely be here
another 10 years.

What are the current thoughts on storage for long lengths of time?
Would it be better to keep the tissues in fixative as opposed to buffer?
Would cacodylate buffer be preferable to phosphate (or is there something
else)? Any special techniques for processing old tissues and getting useable
results? How long have tissues been kept around and still been successfully
worked with?

Thanks for any advice from all the experts on the list.

Rosemary Harris
Electron Microscopy Laboratory
Alberta Environmental Centre






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Wed, 24 Apr 1996 09:18:08 -0500
Subject: Re: Kidney Fixation for Routine TEM

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At 09:02 PM 4/23/96 -0400, you wrote:

} Greetings:
}
} Am interested in finding out the best overall fixation for Kidney (medulla
} and cortex).
} Interested in good preservation (who isnt) as well as defined membrane
} boundries. Specifically looking at mitochondria.
}
}
**********************
We get good results fixing renal biopsies in 3% glutaraldehyde in 0.09 M
PIPES, pH 7.2 followed by 2.0% osmium in PIPES. We then enbloc stain with
saturated aqueous UAc for 45 minutes and embed in Spur's resin. The tissue
remains in the glutaraldehyde for varying times up to a day because these
are medical specimens coming from various hospitals.


Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: Joyce Craig :      Chicago.State.University-at-uxa.ecn.bgu.edu
Date: Wed, 24 Apr 1996 11:04:54 -0500
Subject: critical point dryer problems

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We have a Bal-Tec CPD030. It has been used very little. This is because we
cannot get it to drain. It might take all night for the alcohol-CO2 mixture
to empty. The instructions seem very straigt-forward. We fill partially
with 100% ethanol (with or without a sample), cool to 8 degrees C, let in
the carbon dioxide (yes, the tank is the kind with the dip tube), then press
the medium out button. At first it seems to empty, then gets very slow.
Has anyone had a similar problem? The valves seem to work as I hear a loud
click when pressing buttons. The filters are clean. Any other ideas? thanks.





From: MicroWorld News :      spb-at-wwa.com
Date: Wed, 24 Apr 1996 12:30:01 -0500
Subject: Re: Lehigh course and other courses

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There is a list of microscopy short courses and in-house trainers on the WWW
at http://www.mwrn.com/product/train.htm The list has direct links to
information on the WWW and e-mail addresses. Lehigh information is included.

Susanne Pignolet Brandom
MicroWorld Resources and News
http://www.mwrn.com/
spb-at-wwa.com
847-548-6522

At 04:00 PM 4/23/96 -0400, you wrote:
} Have a customer interested in EM courses. I suggested the Lehigh course.
} Would like to know who to have her contact to get info on the Lehigh course.
} TIA
} Cal Montgomery
}
}
}





From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Wed, 24 Apr 1996 13:23:11 -0700 (PDT)
Subject: long term storage/fixation

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I recall that some NASA sponsored studies of long term fixation found the
best results leaving tissue in the glutaraldehyde fix. This may even have
been presented at an EMSA meeting in the mid 1970's but I haven't found
the reference yet.





From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 24 Apr 1996 17:26:52 -0400 (EDT)
Subject: Re: Standards for EDX on TEM

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}
} If anyone out there has got advice/recommendations/experience on the
} preparation and use of standards for TEM+EDX we would be most
} interested to hear from you.
}
} Though our emphasis will ultimately be on biological applications, any
} input would be of interest at this stage.
}
Dear Tony,
A few years ago, the late, lamented Chuck Fiori produced some
lithium borate glass standards especially designed for average Z to be
equal to that of tissue. I think someone at NIST can tell you more.
Good luck.
Yours,
Bill Tivol




From: Eric Steel :      steel-at-enh.nist.gov
Date: Wed, 24 Apr 1996 12:35:40 -0400 (EDT)
Subject: EDS standards for AEM

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In reply to Tony Bruton's request for info on EDS/TEM standards:

There is a National Institute of Standards and Technology Standard Reference
Materials (SRM) specifically designed for the X-ray analysis on the TEM,
though not for biological applications, and unluckily it is not free.

Here is a brief description of the material, so that you can see if you
might be interested:

The standard is called "SRM 2063 Microanalysis Thin Film" and consists of a
thin film of sputtered mineral glass supported by a carbon film and a copper
TEM grid. The composition is certified as listed below and was determined
by several independent techniques after the glass was deposited on the
grids. Thus the certified composition values take into account sample
preparation. The thickness of the glass (76 nm) and density (3.1 gm/cm**3)
are reported (though not certified). No preparation is necessary for use in
the TEM.

Table of Concentration Values for SRM 2063 Mineral Glass
Element Concentration
(% wt.)
O 43.2
Mg 7.97
Si 25.34
Ca 11.82
Fe 11.06

The composition allows for a range of relative sensitivity values to be
determined for many commonly analyzed elements and x-ray lines. Thus the
standard can be a primary, traceable way of checking other in-house
standards you may use. The standard is robust under many handling and beam
conditions, though very high beam dose may cause a change in composition (as
noted on the certificate of analysis.) We have used one standard grid for
about seven years in our laboratory to monitor/compare several instruments
and detectors.

For more information about obtaining the standard you may contact:

Standard Reference Materials Program
National Institute of Standards and Technology
Gaithersburg, MD 20899-0001
USA

Phone: 301-975-6776
FAX: 301-948-3730
e-mail: SRMINFO-at-enh.nist.gov





From: Paul Webster :      paul.webster-at-yale.edu
Date: 24 Apr 1996 15:47:14 -0400
Subject: Re: Fixation

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I read the current set of postings about "the best fixative for...", and "the
effect of long term storage on..."and see that many researchers are using PIPES
adjusted to a pH above 7.2 to buffer their fixatives.

This buffer has a pKa of 6.8 so would anyone like to make a comment on the
buffering capacity of this buffer above pH 7.0?

If we have to use a "Good" buffer for fixation, we choose HEPES which has a
higher pKa. We get exceptional morphology in cryosections even if we only use
formaldehyde. We also used it at a conc. of 300mM recently to fix some shark
tissue with similar results. We never did get much from using PIPES.

As for tissue storage, there should be no problem in storing tissue for long
periods in fixative, if the samples are to be examined only for morphology. The
samples will slowly get harder and more brittle the longer they are in
gluteraldehyde but if they are embedded in resin it will not be a problem.
There might be some extraction of soluble material with time but that will only
help with increasing the final contrast of the specimen.

If the samples are only to be fixed in formaldehyde then the fixative should not
be removed. I have heard unconfirmed stories of 10 - 20 year old samples,
stored in formaldehyde at RT, being taken off the shelf and successfully labeled
with antibodies. I guess it all depends on what the antigens were.

Best regards

Paul Webster,
Center for Cell Imaging
Yale University School of Medicine.







From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Wed, 24 Apr 1996 11:46:30 -0700 (PDT)
Subject: Re: blocking endogenous peroxidase

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Gary,

The question of which solvent to use depends upon what you are trying to
block. The methanol denatures the peroxidase's catalytic site such
that the peroxide cannot be split or released once it has been bound.
Many years ago I compared peroxidase inhibition on bone marrow needle
biopsies between peroxide solutions with PBS,
ethanol, methanol and acetone. PBS and ethanol gave modest
reduction of endogenous peroxidase, and PBS is probably sufficient
for most tissues. Methanol gave the most complete
inhibition of the variety of peroxidatic activities in marrow. Acetone
actually appeared to potentiate it in some leucocytes


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu



Gary H. Zajic wrote:

} Hello, I am doing immunocytochemical experiments on mouse cochlea
} using frozen sections. I find that I must block endogenous peroxidase
} in these tissues. Most protocols I see require dehydration to 100%
} EtOH or MeOH when blocking with H2O2. Cochlear tissue is very delicate
} and there is a risk losing some of the fine structure with these
} additional steps. My questions are: why can't I just block with H2O2
} in my buffer? is the alcohol necessary for penetration allowing better
} access of the H2O2 to the tissue? These are 10 micron sections.
} Thanks in advance. Sincerely, Gary
}
}
} zajic-at-umich.edu
}





From: PHOBOS11-at-aol.com
Date: Wed, 24 Apr 1996 21:36:21 -0400
Subject: Re: critical point dryer problems

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Joyce,

I think that the inlet diaphram maybe clogged. I am now with RMC and still
help people with problems with BAL-TEC / Balzers equipment. Please feel free
to call me at RMC, 520-889-7900. I can talk you through your difficulties if
you call.

P.S I am available to help any BAL-TEC/ Balzers users out there, just E-Mail
me or call.

Best Regards,

Al Coritz




From: ychen-at-MACC.WISC.EDU
Date: Wed, 24 Apr 1996 16:31:32 -0700
Subject: Re: critical point dryer problems

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} Date: Wed, 24 Apr 1996 11:04:54 -0500
} From: Joyce Craig {Chicago.State.University-at-uxa.ecn.bgu.edu}
} Subject: critical point dryer problems
} To: Microscopy-at-aaem.amc.anl.gov
} X-Sender: bafpjec-at-pop3.ecn.bgu.edu
} X-Mailer: Windows Eudora Light Version 1.5.2
} Mime-Version: 1.0
} Content-Type: text/plain; charset="us-ascii"
}
} We have a Bal-Tec CPD030. It has been used very little. This is because we
} cannot get it to drain. It might take all night for the alcohol-CO2 mixture
} to empty. The instructions seem very straigt-forward. We fill partially
} with 100% ethanol (with or without a sample), cool to 8 degrees C, let in
} the carbon dioxide (yes, the tank is the kind with the dip tube), then press
} the medium out button. At first it seems to empty, then gets very slow.
} Has anyone had a similar problem? The valves seem to work as I hear a loud
} click when pressing buttons. The filters are clean. Any other ideas? thanks.

Joyce,
In my opinion, it looks like the problem is that the exhaust pipe is
jammed. Maybe it is too thin or dirty. If this is the case, in the
beginning it will work fine. After a while, it will freeze and jam. Under
normal conditions, you can hear loud sound and see CO2 gas and ice is
expelled when you open the venting value. If this does not happen, it was
probably jammed.
Ya Chen


Ya Chen

==========================================================================
Cryo/SEM Coordinator
Integrated Microscopy Resource (IMR)-- III M M RRRRRR
an NIH Biomedical Research Resource I M M M M R R
University of Wisconsin, Madison, WI I M M M RRRRRR
1675 Observatory Drive #167 I M M R R
Madison, WI 53706, USA I M M R R
TEL : 608-263-8481 I M M R R
FAX : 608-265-4076 III M M R R
Email:YChen-at-macc.wisc.edu
==========================================================================
IMR WWW Home Page: http://www.bocklabs.wisc.edu/imr.html
2nd Symposium on Integrated Microscopy: Sept. 20-22, 1996






From: Sylvia Francis Zalzal :      franciss-at-ere.umontreal.ca
Date: Wed, 24 Apr 1996 18:33:34 -0400
Subject: Re: critical point dryer problems

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} Date: Wed, 24 Apr 1996 18:28:18
} To: Joyce Craig {Chicago.State.University-at-uxa.ecn.bgu.edu}
} From: Sylvia Francis Zalzal {franciss-at-ere.umontreal.ca}
} Subject: Re: critical point dryer problems
}
} We have in our lab and for over three years the same CPD030 and we never
had this problem. The dehydrant/Co2 exchange should not cause any
trouble,and the sound should not be loud when you depress the buttons.
However we had prior to this Bal-tec 030 we had a cpd020 and a similar
problem occured the reason was " frozen valves " Maybe when cooling the
chamber do not cool under 11 degrees. Try it then call for service check the
valves.
}
} Good luck=20
} Sylvia=20
}
}
}
}
} At 11:04 4/24/96 -0500, you wrote:
} } We have a Bal-Tec CPD030. It has been used very little. This is because=
we
} } cannot get it to drain. It might take all night for the alcohol-CO2=
mixture
} } to empty. The instructions seem very straigt-forward. We fill partially
} } with 100% ethanol (with or without a sample), cool to 8 degrees C, let in
} } the carbon dioxide (yes, the tank is the kind with the dip tube), then=
press
} } the medium out button. At first it seems to empty, then gets very slow. =
=20
} } Has anyone had a similar problem? The valves seem to work as I hear a=
loud
} } click when pressing buttons. The filters are clean. Any other ideas?
thanks.
} }
}
Sylvia Francis Zalzal
Chef de Laboratoire
Laboratoire de Microscopie Electronique
Facult=E9 de m=E9decine dentaire
Universit=E9 de Montr=E9al





From: DENNIS WARD :      dw0005-at-epfl2.epflbalto.org
Date: Thu, 25 Apr 1996 05:09:36 -0400 (EDT)
Subject: Re: ASTM Home Page?

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http://www.astm.org

On 22 Apr 1996, South Bay Technology wrote:

} Can anyone provide me with the URL for the ASTM home page?
}
} Thank you!
}
} Best regards-
}
} David Henriks TEL: 800-728-2233 (toll-free in USA)
} South Bay Technology, Inc. 714-492-2600
} 1120 Via Callejon FAX: 714-492-1499
} San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
} sbt-at-msa.microscopy.com
}
} Manufacturers of Precision Sample Preparation Equipment and Supplies for
} Metallography, Crystallography and Electron Microscopy.
}
}




From: Shelley Jan Almburg :      salmburg-at-biology.lsa.umich.edu
Date: Thu, 25 Apr 1996 08:03:40 -0400 (EDT)
Subject: unsubscribe

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Message-Id: {199604251020.MAA11382-at-elc1.dina.kvl.dk}

please unsubscribe




From: Don Lesher :      72714.265-at-CompuServe.COM
Date: 25 Apr 96 09:03:50 EDT
Subject: ARL SEMQ Microprobe

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Any University interested in obtaining an old, but restorable ARL-SEMQ
Microprobe should contact Advanced MicroBeam at 72714.265-at-compuserve.com or
phone: 330 394-1255, fax: 330 394-1834.


Advanced MicroBeam, Inc.
4217C King Graves Rd.
PO Box 610
Vienna, OH 44473 USA





From: Carmine M. Pariante :      cparian-at-emory.edu
Date: Thu, 25 Apr 1996 10:08:26 -0400 (EDT)
Subject: ARL SEMQ Microprobe

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please unsuscribe

Carmine M. Pariante
cparian-at-emory.edu




From: Kurt.Albertine-at-hsc.utah.edu
Date: Thu, 25 Apr 1996 08:51 -0700 (MST)
Subject: Guidance for selling a TEM or two

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Hello

Jonathan Krupp gave me your eMail address. I have a TEM to sell
and would like to list it on the MSA BB/mail listserver (I am a
member of the MSA). Would you please let me know the format for the
listserver? Also, would you please send instructions for subscribing
to the list via your Web site? Thank you.

Kurt H. Albertine, Ph.D.
Director, Research Microscopy Facility
University of Utah
Health Sciences Center
Salt Lake City, UT 84132

eMail: Kurt.Albertine-at-hsc.utah.edu
FAX: (801) 585-7395
Office: (801) 581-5021




From: Radice-at-urvax.urich.edu (Gary Radice)
Date: Thu, 25 Apr 1996 14:23:15 -0400
Subject: Peltier vs adiabatic CPD

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Alternate-Recipient: prohibited
Auto-Forwarded: prohibited
Content-Return: allowed
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Conversion: allowed
Importance: normal
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Sensitivity: Normal

We are looking to buy a new critical point drier to replace our old one
that cooled adiabatically (by venting CO-2) and notice that some CPDs are
now available that cool electronically by the Peltier effect. This seems
like a big advantage but does anyone with experience with both types care
to comment?

Gary Radice 804-289-8107 (voice)
Department of Biology 804-289-8233 (FAX)
University of Richmond
Richmond VA 23173
USA






From: Joyce Craig[SMTP:Chicago.State.University-at-uxa.ecn.bgu.edu]
Date: Thu, 25 Apr 1996 07:42:04 -0400
Subject: critical point dryer problems

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Message-ID: {01BB3289.A38177E0-at-DENT-BIOMAT2.BU.EDU}
"Microscopy-at-aaem.amc.anl.gov" {Microscopy-at-aaem.amc.anl.gov}

Valve could be freezing. Can you get to it to warm it up?

Ron


----------

We have a Bal-Tec CPD030. It has been used very little. This is because we
cannot get it to drain. It might take all night for the alcohol-CO2 mixture
to empty. The instructions seem very straigt-forward. We fill partially
with 100% ethanol (with or without a sample), cool to 8 degrees C, let in
the carbon dioxide (yes, the tank is the kind with the dip tube), then press
the medium out button. At first it seems to empty, then gets very slow.
Has anyone had a similar problem? The valves seem to work as I hear a loud
click when pressing buttons. The filters are clean. Any other ideas? thanks.








From: rnessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Thu, 25 Apr 1996 09:29:28 -0500 (CDT)
Subject: "Free" Zeiss 10 clarification

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Hi all,
I wanted to post more information about the Zeiss TEM that the
University of Iowa's Biology dpartment would like to give away. I spoke
with Shelley Plattner this morning, as he is in charge of the instrument,
and is the person to contact. He can be reached at (319)-335-1070, or
email at shelley-plattner-at-uiowa.edu. He says that it is a 10A, not a 10C.
Zeiss knows that they are trying to give the scope away, and estimates
that it would take about 40 hours of labor -at-$150.00/hr. Zeiss will make
sure that the instrument works upon relocation. Shipping would be extra,
depending on the carrier and distance. Zeiss might be willing to
discount the cost if the person purchases a service contract. You could
discuss this with them by calling 1-800-233-3334. Lam, the Zeiss service
engineer who has maintained this instrument can be reached at extension
725 at the above 800 number. He can answer question in regards to what
would be needed to hook up the microscope.
Thanks to those who have expressed interest, and have tolerated the run
around on our end.

Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu





From: Louisa.Howard-at-Dartmouth.EDU (Louisa Howard)
Date: 25 Apr 96 09:25:50 EDT
Subject: Re: Long Term Fix/Pipes vs. Hepes

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Message-id: {13851929-at-prancer.Dartmouth.EDU}

---Paul Webster wrote:
I read the current set of postings about "the best fixative for...", and "the
effect of long term storage on..."and see that many researchers are using PIPES
adjusted to a pH above 7.2 to buffer their fixatives.

This buffer has a pKa of 6.8 so would anyone like to make a comment on the
buffering capacity of this buffer above pH 7.0?
--- end of quoted material ---

I agree that HEPES would be much better to use at pH 7-3-7.4, given its pKa of
7.4. We use it often in SEM preps. The reason we use PIPES for TEM, is that it
appears to cause less extraction in mammalian and plant tissues. This is
strictly anectdotal on our part, although Coetzee and van Der Merwe, J of
Microscopy135(2) 147-158, 1984, showed that extraction in plant tissue was
greater using HEPES than using PIPES.
I'm definitely willing to try HEPES buffer on mammalian brain tissue, as other
posters have mentioned using HEPES for TEM, with success. Can I use the same
molarity as with PIPES? I currently use a buffer concentraion of 0.1M PIPES pH
-at-7.3, with a gluatraldehyde concentration of 3-4%.
Also, I agree with other posters about the toxicity of Na Cacodylate, although
the HEPES and PIPES buffers, supposedly have carcinogenic problems. If we work
in EM, we have to take all neceesary precautions, when working with these
compounds. I think the PIPES or HEPES buffers might be a better choice for
field work.
thanks.
Louisa Howard
EM facility- Remsen 240
Dartmouth College
Hanover, NH. 03755




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Thu, 25 Apr 1996 09:32:47 -0500
Subject: Re: "Good" buffers for fixation

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Mime-Version: 1.0
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I must admit I use HEPES buffer, pH 7.2 for my fixatives with great luck
and therefore have not been tempted to change it but I think a good
argument to use PIPES can be made. PIPES has a pKa of 6.8 and HEPES has
one of 7.5. Thus, for a target of pH 7.2, PIPES is 0.4 pH units off and
HEPES is 0.3 units. A good rule of thumb is to use a buffer whose pKa is
within 0.5 units of the desired pH. HEPES may look to be marginally
better than PIPES but fixation solutions tend to acidify during the
fixation step so by having a buffer whose pKa is on the low side of the
starting pH, one would get increased protection at reasonable buffer
concentrations (i.e., the pH would have to drop 0.9 units before being }
0.5 units from PIPES pKa but by only 0.3 units before being } 0.5 units
from HEPES pKa). I think both are "Good" buffers in more than one sense
and both are definitely superior to cacodylate in many ways (e.g., cost,
toxicity, environmental). Furthermore, I believe cacodylate has a pKa of
around 6.3! My feeling has always been the continued prevalence of
cacodylate buffers simply demonstrates that morphologists are some of the
most old-fashioned scientists around and least willing to change. That's
my two cents worth.

} I read the current set of postings about "the best fixative for...", and "the
} effect of long term storage on..."and see that many researchers are using PIPES
} adjusted to a pH above 7.2 to buffer their fixatives.
}
} This buffer has a pKa of 6.8 so would anyone like to make a comment on the
} buffering capacity of this buffer above pH 7.0?
}
} If we have to use a "Good" buffer for fixation, we choose HEPES which has a
} higher pKa. We get exceptional morphology in cryosections even if we only use
} formaldehyde. We also used it at a conc. of 300mM recently to fix some shark
} tissue with similar results. We never did get much from using PIPES.
}
} As for tissue storage, there should be no problem in storing tissue for long
} periods in fixative, if the samples are to be examined only for
} morphology. The
} samples will slowly get harder and more brittle the longer they are in
} gluteraldehyde but if they are embedded in resin it will not be a problem.
} There might be some extraction of soluble material with time but that will only
} help with increasing the final contrast of the specimen.
}
} If the samples are only to be fixed in formaldehyde then the fixative
} should not
} be removed. I have heard unconfirmed stories of 10 - 20 year old samples,
} stored in formaldehyde at RT, being taken off the shelf and successfully
} labeled
} with antibodies. I guess it all depends on what the antigens were.
}
} Best regards
}
} Paul Webster,
} Center for Cell Imaging
} Yale University School of Medicine.

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Richard Sherburne :      richard.sherburne-at-ualberta.ca
Date: Thu, 25 Apr 1996 15:22:29 -0600
Subject: subscription

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Message-ID: {01BB32BB.0589A300-at-valerian.mmid.med.ualberta.ca}

subscribe microscopy





From: CSEDAX-at-ARCRIDE.EDU.AR
Date: Thu, 25 Apr 1996 13:10 -0300
Subject: How to face research and service work within the same lab?

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Hi everyone,

since long ago I've been tempted to ask all of you, specially
to those giving TEM/SEM services as the only work activity, your opinion about
the type of services to offer in an institution which is fully oriented to this
activity:


1) How do you consider giving services in the areas of biological materials and
materials science at the same time?

2) Should one focus the work into specific subjects within a given area?

3) How do you define, in advance, the type of work you would be able to do?
or you wait to be asked for a specific task before deciding to do it
or not?

These may sound elemental questions to you but I've never had the chance
to hear people from other systems to give their opinion on that.



Now, for those doing research and giving services...

A) How do you combine both activities?

B) How much time, or effort, do you put on each one of these activities?

C) About the services to offer, should they be only related to the subject
of your research work?


I would appreciate very much any comments and I promise
to make a compilation of all answers for those interested in the subject.
Thanks in advance.

............................................................................
Silvia Montoro
Centro Regional de Investigacion y Desarrollo
Santa Fe
Argentina

csedax-at-arcride.edu.ar





From: p.marks-at-unsw.edu.au (patrick marks)
Date: Fri, 26 Apr 1996 10:40:48 +1000
Subject: How to face research and service work within the same lab?

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Hi Netter,

I am working with kangaroo heart muscle, and my aim is to find mitochondrial
volume density and also mitochondrial numbers, but I'm having a problem with
the uranyl acetate and lead citrate staining. I am staining with 4% uranyl
acetate for 1 hour under a lamp, then lead citrate for 4 minutes, yet my
sections are very pale under the transmission microscope, with very little
contrast in many cases. Has anyone else had this problem? I am wondering if
this is a characteristic of this kind of muscle or if it is me and my
methods! If you have any suggestions please RESPOND TO


Dr. SUNIL TEWARI
S.Tewari-at-unsw.edu.au
Patrick Marks
Electron Microscope Unit
University of New South Wales
Sydney 2053
Australia
Email : P.Marks-at-unsw.edu.au





From: p.marks-at-unsw.edu.au (patrick marks)
Date: Fri, 26 Apr 1996 10:45:59 +1000
Subject: Grid staining problem

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} To: microscopy-at-aaem.amc.anl.gov
} From: p.marks-at-unsw.edu.au (patrick marks)
}
} Hi Netter,
}
} I am working with kangaroo heart muscle, and my aim is to find
mitochondrial volume density and also mitochondrial numbers, but I'm having
a problem with the uranyl acetate and lead citrate staining. I am staining
with 4% uranyl acetate for 1 hour under a lamp, then lead citrate for 4
minutes, yet my sections are very pale under the transmission microscope,
with very little contrast in many cases. Has anyone else had this problem? I
am wondering if this is a characteristic of this kind of muscle or if it is
me and my methods! If you have any suggestions please RESPOND TO
}
}
} Dr. SUNIL TEWARI
} S.Tewari-at-unsw.edu.au
}
Patrick Marks
Electron Microscope Unit
University of New South Wales
Sydney 2053
Australia
Email : P.Marks-at-unsw.edu.au





From: Douglas F Bowling :      al428-at-dayton.wright.edu
Date: Thu, 25 Apr 1996 22:58:32 -0400
Subject: unsubscribe

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unsubscribe




From: peling-at-amnh.org (Peling Melville - Interdepartmental Facilities)
Date: Fri, 26 Apr 1996 08:03:34 -0500
Subject: Re: critical point dryer problems

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Message-Id: {v01510100ada679a5be48-at-[198.116.4.5]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} On Wednesday April 24th, Joyce Craig writes:
}
} } We have a Bal-Tec CPD030. It has been used very little. This is because we
} } cannot get it to drain. It might take all night for the alcohol-CO2 mixture
} } to empty. The instructions seem very straigt-forward. We fill partially
} } with 100% ethanol (with or without a sample), cool to 8 degrees C, let in
} } the carbon dioxide (yes, the tank is the kind with the dip tube), then press
} } the medium out button. At first it seems to empty, then gets very slow.
} } Has anyone had a similar problem? The valves seem to work as I hear a loud
} } click when pressing buttons. The filters are clean. Any other ideas?
} } thanks.
}
}
} Hi Joyce,
}
} We have a Balzers CPD030 and I've experienced the same problem that you
} have. What I have had to do is check the inlet diaphram. 9 times out of
} 10 something has clogged it. I'll take it out and holding the diaphram
} firmly, I "blast" it with an air duster. That usually does the trick.
}
} Peling Melville
}
} --------------------------------------------------------------
} Peling Fong Melville
} Senior Scientific Assistant
} Interdepartmental Facilities
} American Museum of Natural History
} Central Park West at 79th Street
} New York, NY 10024-5192 U.S.A.
} ******************************
} E-mail: peling-at-amnh.org
} Work #: (212) 769-5469
} FAX #: (212) 769-5495

--------------------------------------------------------------
Peling Fong Melville
Senior Scientific Assistant
Interdepartmental Facilities
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 U.S.A.
******************************
E-mail: peling-at-amnh.org
Work #: (212) 769-5469
FAX #: (212) 769-5495






From: brannign-at-asrr.arsusda.gov (Peggy Brannigan)
Date: Fri, 26 Apr 1996 08:46:01 -0400
Subject: Re: low contrast with lead citrate

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Hello microscopists,

Sunil Tewar asked for some advice about lead citrate and low contrast - I
could also use some tips!
My problem is that lead citrate gives variable and unpredictable contrast
in the TEM. I am using LX 112 (plant tissue),osmium, and 4% uranyl acetate
for 45-60 minutes; over the years lead citrate staining times have varied
from 8 min to 25 min. Right now I'm staining around 15 min - was at 18
for a while but suddenly everything was too dense.

I 've read that the pH of the final solution is very important - that it
should be pH 12.0 +/ 1 0.1 When I make it up however the pH is never less
than 12.4! I 've been reluctant to introduce HCl into the picture so I
haven't yet tried reducing the pH, consequently don't know if this is the
problem. I do know that the amount of NaOH in the rinse water is very
important - I use about 5 drops of 1N in 40 mls of water - more than that
bleaches out the tissue. ( Can't remember off the top of my head what that
means in terms of pH). There appears to be no connection between staining
problems and the batch of resin, the type of tissue or the fixation.

One interesting note: when I moved to a new building a year ago, I
couldn't get any staining at all for three months! Non microscopists in
the building were also having unrelated problems, ultimately traced to
impurities in our central distilled water source. Once this was fixed our
problems disappeared.

Has anyone else had problems getting contrast with lead citrate? If so,
how did you fix it?

Thanks, Peggy .

} Hi Netter,
}
} I am working with kangaroo heart muscle, and my aim is to find mitochondrial
} volume density and also mitochondrial numbers, but I'm having a problem with
} the uranyl acetate and lead citrate staining. I am staining with 4% uranyl
} acetate for 1 hour under a lamp, then lead citrate for 4 minutes, yet my
} sections are very pale under the transmission microscope, with very little
} contrast in many cases. Has anyone else had this problem? I am wondering if
} this is a characteristic of this kind of muscle or if it is me and my
} methods! If you have any suggestions please RESPOND TO
}
}
} Dr. SUNIL TEWARI
} S.Tewari-at-unsw.edu.au
} Patrick Marks
} Electron Microscope Unit
} University of New South Wales
} Sydney 2053
} Australia
} Email : P.Marks-at-unsw.edu.au






From: kna101-at-utdallas.edu
Date: Fri, 26 Apr 1996 09:47:03 -0500 (CDT)
Subject: Re: "Good" buffers for fixation

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Tom,
I just had to let you know that you made my day. The comment about
microscopists being resistant to change describes my boss to a T. He
recently gave me the task of determining the best way to screen for
mucins in tissue sections, and when I came back, after reading ALOT of
info on mucins, with the suggestion of using lectin staining and
immunohistochemistry (which we have done before for other material), he
pronounced that I would have to work out a technique using ONLY
histochemistry, as he felt the other methods wouldn't work.
Karen P.




From: delphi.beckman.uiuc.edu-at-delphi.beckman.uiuc.edu (steve rogers)
Date: Fri, 26 Apr 1996 10:43:39 -0500
Subject: PIPES for fixation

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} I read the current set of postings about "the best fixative for...", and "the
} effect of long term storage on..."and see that many researchers are using PIPES
} adjusted to a pH above 7.2 to buffer their fixatives.
}
} This buffer has a pKa of 6.8 so would anyone like to make a comment on the
} buffering capacity of this buffer above pH 7.0?

PIPES is one of a few buffers suitable for work with cytoskeletal
components (e.g. microtubules). It is often used to buffer fixativesfor
light & electron microscopy to preserve cytoskeletal structure.


******************************************************
Steve Rogers
Dept. of Cell & Structural Biology and the
Beckman Institute - Optical Visualization Facility
University of Illinois -at- C/U
srogers-at-delphi.beckman.uiuc.edu
******************************************************






From: Charles.P.Daghlian-at-Dartmouth.EDU (Charles P. Daghlian)
Date: 26 Apr 96 12:19:33 EDT
Subject: Sputter coater and parts for JSM T-300

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Message-id: {13923531-at-prancer.Dartmouth.EDU}

I am helping the Montpelier (Vermont) High School maintain and use a JSM T-300
SEM as part of the science labs. The SEM is linked via its TV output to a Scion
frame grabber in a Macintosh running NIH Image. The science teaching with whom
I am working, Dave McGraw, is involved with the UA Center for Image processing
in Teaching, so this fits in with things that are already being done in the
school. All is well except we need two things.
1. If there is a used T-300 out there that is available for the cost of
shipping, we could use it for spare parts.
2. We are in need of a used sputter coater, too. We have a vacuum pump and can
make some repairs. If you have one available, please let me know.

Thanks in advance to any who respond.

Chuck
*************
Charles P. Daghlian, Ph.D.
Director, Rippel E. M. Facility
Dartmouth College, HB 7605
Hanover, NH 03755
phone 603-646-1039




From: LYNNE (617) 386-1446 :      GARONEL-at-cliffy.polaroid.com
Date: Fri, 26 Apr 1996 14:25:36 -0400 (EDT)
Subject: Open Job Req. at Polaroid for TEM - polymer microscopist

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Polaroid Corp. in Waltham, Ma. USA is looking for a Ph.D. or M.S. in chemistry, materials science, physics or engineering with training in polymer science and
experience in TEM and sample prep. SEM, LM, XPS, XRD, AFM all plusses. STRONG Communication skills and computer skills desirable. Send inquiries to:Lynne
Garone E. Mail:GaroneL-at-Polaroid.com




From: Douglas F Bowling :      al428-at-dayton.wright.edu
Date: Thu, 25 Apr 1996 22:58:32 -0400
Subject: unsubscribe

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From: kna101-at-utdallas.edu
Date: Fri, 26 Apr 1996 14:44:50 -0500 (CDT)
Subject: Re: "Good" buffers for fixation

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I would like to apologize if I affended anyone on this list with my
"airing" my frustrations. It was inappropriate. Tom's comment just
struck a cord. I realize setting up new protocols for any one tissue is
time consuming and often the "why fix it if it works" attitude is the
best. But, sometimes the old ways don't work as well as you need them
to, then new methods may be necessary.

Karen




From: James S MArtin :      James.S.Martin-at-williams.edu
Date: Fri, 26 Apr 1996 15:32:50 -0400 (EDT)
Subject: microscope hot stages

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Seeking ideas for building a low cost microscope hot stage. It doesn't
have to be pretty, only accurate and reliable. We're a small non-profit
museum lab, so I will only ever see a Mettler stage in my dreams. I have
scavenged most of the components necessary for generating and control T
from a PE GC and a melting point apparatus, but am looking for a design
for the stage and insulator jacket (probably water flow).

Any thoughts appreciated. Thanks.

James Martin
Director of Analytical Services and Research
Williamstown Art Conservation Center




From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Fri, 26 Apr 1996 10:35:20 -0500
Subject: MMS Spring Symposium

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Announcement:

**************************************************************

The 1996 Minnesota Microscopy Society Annual SPRING SYMPOSIUM

**************************************************************

SHERATON INN, MIDWAY, I-94 at HAMLINE AVENUE, ST. PAUL, MN

THURSDAY - MAY 23, 1996

**************************************************************

SCHEDULE OF EVENTS

8:00 - 9:00 AM Coffee and Late Registration

9:00 - 9:45 AM Understanding Video Signals: Cameras, Recording Formats and
Printers
MARTY HARALDSON, Alpha Video, Edina, MN

9:45 - 10:30 AM Coffee Break \ Vendor Displays

10:30 - 11:15 AM Capturing, Storing and Organizing Digital Image Files
MARK SANDERS, Imaging Center, College of Biological Sciences
University of Minnesota, St.Paul Campus

11:15 - 12:45 PM A buffet lunch will be served at the Sheraton Inn

12:45 - 1:00 PM MMS Business Meeting and Election of Officers

1:00 - 1:45 PM Microscopy Resources on the Internet
Dr. STUART MCKERNAN, Center for Interfacial Engineering,
Characterization Facility, University of Minnesota

1:45 - 2:15 PM Coffee Break \ Vendor Displays

2:15 - 3:00 PM Public Domain Shareware, Microscopy and the Internet
Dr. DAVID BRIGHT, 1996 MSA Traveling Speaker
Research Chemist, Microanalysis Group
NIST, Gaithersburg, MD

3:00 - 4:30 PM Vendor Displays

*********************************************************************
Please make your reservation in advance! NO LATER THAN MONDAY, MAY 20, if you
plan to attend the Symposium (preferred, or pay at the door).

Contact Stuart McKernan at (612) 626-7942, stuartm-at-maroon.tc.umn.edu or
Dwight Erickson at (612) 736-2830, usmmm214-at-ibmmail.com

Symposium Fee:
$20.00 current regular MMS members 95/96, $10.00 student members 95/96
$30 non-member(confers regular membership),
$15.00 non-member students (confers student membership).

*********************************************************************

Vendors: A number of vendors of digital and other microscopy equipment will be
present at the Symposium. If your company would like to have table space for
product display, please contact Symposium Vendor Liaison: Diana Kittleson at
Pillsbury TPC Labs, 330 University Ave. S. E., Minneapolis, MN 55414,
Phone: (612)330-1898 Fax: (612)330-8266

**********************************************************************


Stuart McKernan stuartm-at-maroon.tc.umn.edu
CIE Microscopy Center, University of Minnesota Office: (612) 626-7942
100 Union Street S. E., Minneapolis, MN 55455 Lab: (612) 624-6590





From: beebed-at-alize.ERE.UMontreal.CA (Dwight Beebe)
Date: Fri, 26 Apr 1996 11:47:10 -0500
Subject: Re: "Good" buffers for fixation

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Tom Phillips said:
} I must admit I use HEPES buffer, pH 7.2 for my fixatives with great luck
} and therefore have not been tempted to change it but I think a good
} argument to use PIPES can be made.

Hi,
I'd like to chime in on this thread. I have been using PIPES as a
buffer, but only in fixations for immunolocalizations. I had used it for
morphological studies, but following a conversation with MV Parthasarathy
(EM Director, Cornell), I switched back to either phosphate or cacodylate.
My original interest stems from the following report: Salema R, I Brandao
1973 The use of PIPES buffer in the fixation of plant cells for electron
microscopy. J Submicr Cytol 5:79-96. As I work exclusively with plant
material, primarily leaves, it appeared to be best. The paper presents
results indicatng that PIPES is the least extractive of buffers currently
in use at the time. However, the work by Coetzee and van der Merwe (Coetzee
J, CF van der Merwe 1987 Some characteristics of the buffer vehicle in
glutaraldehyde-based fixatives. J Microsc 146:143-155.) indicates that the
least extraction was found with phosphate-buffered glutaraldehyde. Again,
their work was conducted using plant material (bean leaves) and may not be
directly applicable to animal tissue, which was the subject of the original
question. As most people know, phosphate is not compatible with added
salts, hence, cacodylate would then be the buffer of choice. I would agree
that morphologists are conservative in their approach to new techniques. I
was extremely sceptical of the use of microwaves in fixations, until I was
able to use one of the units myself. I am now convinced that this is a
very powerful technique and yields fixation images equal to or superior to
conventional protocols. The time-saving aspect is a tremendous bonus! I
was loaned a Pelco unit by Phillip Slakmon of Scott Scientific for a number
of months and have used it for more than a dozen fixations, which included
standard protocols for both morphological and immunocytochemical studies.
I have polymerized Spurr's resin, Epon-Araldite, LR White, and mixtures of
Spurr and Epon, all with success (good infiltration, polymerization,
sectioning, staining, and stability in the beam as criteria). Note that
the polymerizations were conducted under water (!) in BEEM capsules. Has
to be tried to be believed, I guess.
Anyway, I would hope that Partha would add his comments to this
discussion on buffers, particularly regarding the suitability of PIPES for
morphological work.


Dwight U. Beebe E-mail: beebed-at-ere.umontreal=
.ca
Institut de recherche en biologie v=E9g=E9tale Voice: 514-872-4563
Universit=E9 de Montr=E9al FAX: 514-872-9406
4101, rue Sherbrooke est
Montr=E9al, Qu=E9bec H1X 2B2
Canada






From: Joyce Craig :      Chicago.State.University-at-uxa.ecn.bgu.edu
Date: Fri, 26 Apr 1996 16:34:40 -0500
Subject: Halobacterium

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Message-Id: {199604262237.RAA01018-at-uxa.ecn.bgu.edu}
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Is anyone out there working with halobacteria? Does anyone have pictures?





From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Fri, 26 Apr 1996 17:37:25 -0600
Subject: Looking for polarizing stereo viewers

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We need to find a supplier of stereo viewers, the cardboard-mounted
polarizing kind. I've looked in every EM supply house catalog that I have
and can't find them anymore. I'd appreciate any help in finding these.
Thanks.

John
chandler-at-lamar.ColoState.EDU






From: James S MArtin :      James.S.Martin-at-williams.edu
Date: Fri, 26 Apr 1996 22:34:28 -0400 (EDT)
Subject: for microscope manufacturers

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Several months ago I posted a request for microscope manufacturers to
contact me with information on costs for epi-fluorescence upgrades to
polarizing light microscopes. The information will be presented at a
national conference.

So far, I have information only from Olympus. Anybody else like to be
represented?

James Martin
Director of Analytical Services and Research
Williamstown Art Conservation Center
Williamstown, MA




From: cmurphy-at-GGPL.ARSUSDA.GOV
Date: Fri, 26 Apr 1996 14:02:24 -0400
Subject: frozen osmium

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Greetings fellow microscopist: Our usually dependable refrigerator turned on us one day recently. It dropped to 0C and our 4%
aqueous osmium tetroxide froze. Does anyone know if this is still good to use. Please drop me a line if anyone has had similar
experiences. Thanks Charlie Murphy




From: Mike Folsom :      mwfolsom-at-unm.edu
Date: Sat, 27 Apr 1996 15:08:57 -0600 (MDT)
Subject: Re: frozen osmium

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To: microscopy-at-Sparc5.Microscopy.Com

On Fri, 26 Apr 1996 cmurphy-at-GGPL.ARSUSDA.GOV wrote:

} Greetings fellow microscopist: Our usually dependable refrigerator turned on us one day recently. It dropped to 0C and our 4%
} aqueous osmium tetroxide froze. Does anyone know if this is still good to use. Please drop me a line if anyone has had similar
} experiences. Thanks Charlie Murphy
}

Yes -

I usually keep my osmium solutions frozen. They appear to work just fine -

Michael






From: rgwhite-at-vaxc.cc.monash.edu.au (Rosemary White)
Date: Sun, 28 Apr 1996 14:31:08 +1200
Subject: Re: low contrast with lead citrate

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We have also had this problem intermittently. It's almost always been
solved by making up new lead citrate, being scrupulous about purity of
distilled water, following the recipe exactly, and making sure the pH is
above 11 (usually ends up higher than this anyway - but may not stay that
high). There are various lead citrate recipes, once we had to switch to
the original Reynolds recipe for some reason, after a while someone tried
the usual recipe and it worked again.... Staining times have varied too,
usually it depends on how old the lead solution is - increasing time for
older solutions.

With plant and animal tissue, we use alcoholic U acetate for only 10-15 min
(made fresh from 4% aqueous - add equal quantities of 4% UA, distilled
water and 100% ethanol, leave for 10 min till no more bubbles), and no NaOH
in rinse water - we just warm it slightly - too warm and sections lose
contrast.

good luck!


Rosemary White
__ /
Department of Ecology _/ \__/ \
and Evolutionary Biology / \
Monash University / Australia \
Clayton, Victoria 3168 \ ____ /
phone 61-3-9905 5670 \_/ \_*_/
fax 61-3-9905 5613 __
email rgwhite-at-vaxc.cc.monash.edu.au \/






From: Vladimir.Oleshko :      oleshko-at-uia.ua.ac.be
Date: Sun, 28 Apr 1996 15:48:30 +0200 (MET DST)
Subject: Re: Open Job Req. at Polaroid for TEM - polymer microscopist

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On Fri, 26 Apr 1996, LYNNE (617) 386-1446 wrote:

} Polaroid Corp. in Waltham, Ma. USA is looking for a Ph.D. or M.S. in
chemistry, materials science, physics or engineering with training in
polymer science and=20
} experience in TEM and sample prep. SEM, LM, XPS,
XRD, AFM all plusses. STRONG Communication skills and computer skills
desirable. Send inquiries to:Lynne=20
} Garone E. Mail:GaroneL-at-Polaroid.com
} =20

Dear Sir,

I would like to express my interest in your proposal. Enclosed is my=20
resume. As it indicates, I have a solid physicochemical background, both=20
from my education at the Moscow University and my subsequent professional=
=20
experience including training in polymer science.

I am currently working as a visiting scientist at the Department of=20
Chemistry, University of Antwerp (U.I.A.), Belgium in close collaboration=
=20
with Prof. Dr. Renaat Gijbels and Prof. Dr. Willem Jacob. At this=20
department well as earlier at the Photochemistry Department of the=20
N.N.Semenov Institute of Chemical Physics, Russian Academy of Sciences
I am primarily involved in analytical electron microscopy studies of=20
silver halide photographic systems, colloids and small particles.

I am familiar with the following methods and techniques: conventional and=
=20
energy-filtering TEM, SEM, STEM, EDX, EELS, image analysis, IR-,=20
UV-VIS-spectrometry, Raman-spectroscopy, electron spin resonance,=20
scanning tunneling microscopy, working with PC.

I am deeply impressed by your proposal. I am hard working, and I feel=20
strongly that my motivation and enthusiasm could be of benefit to your=20
programs.

I look forward to hearing from you.

Sincerely Yours,

Vladimir Oleshko
=20
Curriculum Vitae of Vladimir P. Oleshko, Ph.D.
Personal data
Date of birth: September 19, 1953.
Place of birth: Kobishcha, Bobrovitsky district,
Chernigovsky region, Ukraine.
Martial status: married with son and daughter.
Fluent in Russian and in English.

Permanent address: Photochemistry Department, N.N. Semenov Institute of
Chemical Physics, Russian Academy of Sciences, Novatorov Str. 7a, 117421
Moscow, Russia. =20
Tel.: 7 (095) 9363950 (office); Fax: 7 (095) 936 3950;=20
Tel.: 7 (095) 5504730 (home).=20

Current address: Micro- and Trace Analys is Centre (MiTAC), Chemistry
Department, University of Antwerp (U.I.A.), Universiteitslpein 1, B-2610
Antwerpen-Wilrijk, Belgium. Tel.: +32-3-820.23.64; Fax: +32-3-820.23.76;=20
E-mail: oleshko-at-uia.ua.ac.be=20

Education=20
1974 B.S. (Chemistry) Moscow State University, Moscow, USSR. Honours in
Chemistry.=20

1976 M.S. (Physical Chemistry) Moscow State University, Moscow, USSR.=20
Honours in Physical Chemistry.=20

1983 Ph.D. (Chemistry) Moscow State University, Moscow, USSR.=20

Ph.D. Thesis "Application of spin trapping to investigation of
adsorption and catalytic processes".

Professional Experience
1971-1976: Student, Chemistry Department, Moscow State University.=20
Extended university courses and practical training in inorganic,
analytical, organic and physical chemistry, polymer science and chemical
technology and additional extended courses in high mathematics, physics
and physical chemistry (catalysis and chemical kinetics, adsorption
phenomena, electrochemistry, physical chemistry of solid state, quantum
chemistry, and physical instrumental methods in chemistry).=20

1976-1982: Postgraduate student, N. I. Kobozev Laboratory of Catalysis
and Gas Electrochemistry, Physical Chemistry Division, Chemistry
Department, Moscow State University.=20
Experience in physical chemistry, catalysis, adsorption phenomena,
kinetics of chemical reactions, vacuum and adsorption techniques and
characterization of oxide based catalytic system s by electron spin
resonance using spin probe and spin trap techniques, infrared-, and
electron spect rometry in the ultraviolet and visible regions and computer
simulation of donor-acceptor interactions on the oxide surfaces.=20

1982-1984: Junior Research Fellow, Research Institute of Chemical
Technology, Moscow region, USSR.=20
Experience in polymer science of composite polymer systems and
characterization of polymer molecular dynamics and stability by spin probe
technique.=20

1984-1988: Head of research group, Senior Research Fellow, Experimental
Designing Bureau "Horizon", Moscow, USSR. =20
Experience in materials science and characterization of superconductors
(Nb-Ti alloys, Nb3Sn, and high-temperature superconductor Y-Ba-Cu-O
systems) by scanning (SEM) and transmission electron microscopy (TEM) and
energy-dispersive X-ray microanalysis (EDX).=20

1988-1993: He ad of research group, Senior Research Fellow,
Photochemistry Department, N.N.Semenov Institute of Chemical Physics,
Russian Academy of Sciences, Moscow, Russia. =20
Experience in imaging science and chemical physics of record media (silver
halide photographic systems, thin films, small particles and nanoclusters,
photosensitive semiconductor dispersions and their characterization by
analytical electron microscopy (AEM) methods (TEM/SEM/STEM/electron
diffraction/EDX), image analysis and scanning tunneling microscopy.=20

1992.08.-11.: Visiting Scientist, Korea Research Institute of Chemical
Technology, Daedeog-dangi, Taejeon, South Korea. =20
Experience in photographic science and technology of silver halide
emulsions.=20

1993.08.-to present: Visiting Scientist, Micro- and Trace Analysis Centre
(MiTAC), Chemistry Department, University of Antwerp (U.I.A.),
Antwerpen-Wilrijk, Belgium. =20
Experience in energy-filtering transmission electron microscopy (EFTEM)
and electron energy-loss spectroscopy (EELS) and scanning
energy-dispersive X-ray microanalysis (STEM/EDX) of disperse many-particle
systems (silver-halide-based photographic systems, metal and metal sulfide
colloids, polynuclear cluster coordination compounds, nanocrystalline
films) and Monte Carlo simulations of electron beam-solid interactions.=20

Number of papers: 51 scientific publications.

Invited reviews: Characterization of complex silver halide photographic
systems by means of analytical electron microscopy //Microbeam Analysis.
1995. V.4. N.1, pp. 1-29 (first author).=20

Scanning Microanalysis: In Handbook of Microscopy. Eds. by S. Amelinckx et
al., VCH, 39 p. 14 ill. (1996) (submitted, first author).

Presentations:=20
20 presentations at international and national scientific congresses and
conferences.=20

Research interests
1. Surface phenomena, adsorption, photochemistry, photophysics and catalysi=
s=20
at surfaces of solids, nanochemistry and nanophysics of molecular organized=
=20
systems.

2. Analytical electron microscopy (energy-filtering TEM/ESD/EELS,
STEM/SEM/EDX), image analysis and scanning tunneling microscopy of
disperse matter.=20

3. Formation of ultradisperse metal and metal sulfide phases on
microcrystals of silver halide emulsions at different stages of the
photographic process i.e., ripening, exposure, and development.=20

4. Cluster formation, aggregation and transformations in disperse
many-particle-systems (silver halide emulsions, polynuclear cluster
coordination compounds, colloids, nanocrystals, semiconductor dispersions,
mechanically activated high-temperature superconductors).=20

Main professional accomplishments=20
1. Elucidation of mechanisms of acid-base interactions on oxide surfaces
during radical catalytic reaction by spin traps and spin probes.=20

2. Development of a technique for evaluation of a surface oxide bacidity
by the probing reaction of catalytic radical decomposition of
2-methyl-2-nitrosopropane.

3. Development of a technique for estimation of fine structure, core,
shell and outer sizes of drops of color coupler dispersions in color
photograph ic materials by combined positive-negative staining of
ultrathin sections following stereological reconstruction of volume sizes.=
=20

4. Elucidation of mechanisms of selective and non-selective reduction of
silver halide emulsions with a series of developers (black-and-white,
color, volume and surface developers) and with NaBH4. Demonstration of
formation of percolation networks of silver filaments and particles in the
course of development with an active volume developer.

5. Demonstration of fractal aggregation of products of chemical
sensitization on tabular microcrystals of silver halide emulsions in the
course of the ripening.=20

6. Development of the optimal strategy of structural and analytical
characterization of complex silver halide photographic systems by the
combination of AEM and image analysis techniques.=20

7. Evaluation of crystalline and electronic structure and elemental
composition of advanced double structure tabular microcrystals of silver
halide emulsions by the combination of cryo-EFTEM/ESD/EELS and
cryo-STEM/EDX techniques. Development of a modified log-ratio EELS
technique for estimation of the local crystal thickness.=20

Professional Societies=20
Russian D. I. Mendeleev Chemical Society=20
Belgian Society for Microscopy
European Microanalysis Society

References=20
Prof. Dr. Renaat H. Gijbels=20
Micro- and Trace Analysis Centre (MiTAC), Co-Director
Department of Chemistry, University of Antwerp (U.I.A.),
Universiteitsplein, 1, B-2610 Antwerpen-Wilrijk, Belgium.=20
Tel.: +32-3-820.23.60 Fax: +32-3-820.23.76

Prof. Dr. Willem A. Jacob=20
Electron Microscopy Centre, Head
Department of Medicine, University of Antwerp (U.I.A.),
Universiteitsplein, 1, B-2610 Antwerpen-Wilrijk, Belgium.
Tel.: +32-3-820.25.09 Fax: +32-3-820.26.03=20

Prof. Dr. Gustaaf Van Tendeloo
EMAT, University of Antwerp (R.U.C.A.),=20
Groenenborgerlaan 171, B-2020
Antwerp, Belgium.
Tel.: +32-3-218.02.62 Fax: +32-3-218.02.57.

Prof. Dr. Valery V. Lunin
Department of Chemistry, Dean
Moscow State University,
Vorob=92evy Gory, 119899 Ms cow, Russia
Tel.: 7 (095) 939 45 75, 7 (095) 939 35 71
Fax: 7 (095) 932 00 67.

Prof. Dr. Michael V. Alfimov
Department of Photochemistry, Director
N.N. Semenov Institute of Chemical Physics,
Russian Academy of Sciences Novatorov Str., 7a,=20
117421 Moscow , Russia.
Tel: 7(095) 936 77 53 Fax: 7(095) 936 12 55.





From: Stephen Edgar :      s.edgar-at-auckland.ac.nz
Date: Mon, 29 Apr 1996 10:13:03 NZS
Subject: Re: venting print processors

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The following book may be of interest in regard to this discussion
thread:

"Health Hazards for Photographers" by Siegfried & Wolfgang Rempel.
Published by Lyonn (? may be an abbreviation) in 1993.
ISBN 1558211810

I have not seen this text myself as it is too expensive in this part
of the world - only $16-95 in the US though.

Perhaps Donna Wagahoff's building engineer was thinking of X-ray
processors rather than ordinary photographic machines when he
expressed concern over the extraction of fumes. I have no experience
of X-ray processors myself, but understand that they have caused
problems for a lot of users - isn't formaldehyde used as a hardener
in these machines?


Regards

Stephen Edgar

Electron Microscope Unit, Pathology Department
School of Medicine
University of Auckland
Private Bag 92019
Auckland
New Zealand

email address: s.edgar-at-auckland.ac.nz
Phone : +64-9-3737599 extn 6473 (GMT + 12h)
Fax : +64-9-3737459




From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Sun, 28 Apr 1996 10:45:51 -0600
Subject: Re: Looking for polarizing stereo viewers

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X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
Message-Id: {v02130500ada94fc6a1fa-at-[131.230.97.69]}
Mime-Version: 1.0
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John Chandler posed the following question:

} We need to find a supplier of stereo viewers, the cardboard-mounted
} polarizing kind. I've looked in every EM supply house catalog that I have
} and can't find them anymore. I'd appreciate any help in finding these.
} Thanks.
}

One possible solution:

Reel 3-d Enterprises Inc...Po Box 2368...Culver City, CA 90231-2368
Phone: (310)837-2368


I just posed a similar question for red/greed glasses. Several kind
respondants gave me the name of the above company which sells all kind of
3D materials - including the polarizing glasses. I received the r/g glasses
the next day. They accept charge cards or checks only (no PO's, etc).
Prices are so reasonable, that I just ordered them on my own charge card.

I am just a happy patron with no financial interests in the company.

Peace -



#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Brett W. Cockman :      bcockman-at-uniwa.uwa.edu.au
Date: Mon, 29 Apr 1996 10:03:32 +0700 (WAST)
Subject: RE: frozen osmium

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Message-Id: {199604290027.TAA02614-at-Sparc5.Microscopy.Com}

Hello Charlie,
I store my aqueous osmium solutions (usually 4%) in a frozen state and have
had no problems to date. It is a convenient way to keep the osmium over a
longer period as I only need to use it occasionally and saves the fridge
interior from being inadvertently redecorated with the 'black' precipitate.
Regards,
.
.
Brett W. Cockman
Technologist in Charge
School of Dentistry
University of Western Australia
Voice: (619-2205834)
Fax: (619-2213829)
e-mail; bcockman-at-uniwa.uwa.edu.au




From: GeoffA :      geoffa-at-amsg.austmus.oz.au
Date: 24/4/96 8:59 AM
Subject: long term storage

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To expand upon Louisa Howard's question of long term storage -she is
asking about storage in cacodylate buffer.

The question has recently been asked here about long term storage in
phosphate buffers. We have a variety of fish, rat and cow tissues that were
collected (or will be collected) "just in case" EM may be needed. Some of
these tissues have been around for at least 10 years and will likely be here
another 10 years.

What are the current thoughts on storage for long lengths of time?
Would it be better to keep the tissues in fixative as opposed to buffer?
Would cacodylate buffer be preferable to phosphate (or is there something
else)? Any special techniques for processing old tissues and getting useable
results? How long have tissues been kept around and still been successfully
worked with?

Thanks for any advice from all the experts on the list.

Rosemary Harris
Electron Microscopy Laboratory
Alberta Environmental Centre





From: em-at-mediacity.com (Ed Monberg)
Date: Sun, 28 Apr 1996 23:32:32 -0800
Subject: Re: venting print processors

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Message-Id: {v01540b01adaa1e640d84-at-[205.216.172.10]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} The following book may be of interest in regard to this discussion
} thread: etc.


Gee, guys, doesn't anyone read the labels?

. . and take a browse in the stacks in "toxicology" ?


(Can it be much more complex,

but a lot more dangerous I suspect, than that,

When you can later "taste" the chemicals wetting your skin,

as I have experienced myself.) Sorry about the sentence,

but those chemicals are getting to my brain.



Regards,



(signed) Ed Monberg {em-at-mediacity.com}

--------------------------------------------------

510-429-1060 Fax 429-1065
LMDC, (Laser Motion Development Co.)
3101 Whipple Road
Union City, CA 94587-1216


For our Most recent Catalogue of "On Hand" EQUIPMENT:
Send empty mail to: {Cat-at-lasermotion.com}


Our web page: http://www.lasermotion.com (Is beginning to take shape!)
Our e-mail: office-at-lasermotion.com

{-------------------------------- Our page width
-----------------------------}






From: Koenraad.Janssens-at-mtm.kuleuven.ac.be (Koenraad Janssens)
Date: Mon, 29 Apr 1996 09:35:34 +0200
Subject: SIMCON software, shareware?

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Dear Fellow Microscopists,

The last few years I have been involved in research on localized (near
nanometer resolution) strain characterization using two-beam electron
diffraction contrast imaging (an operational mode of transmission
electron microscopy). See any book on transmission electron microscopy
for the basics.

Part of this project was to develop a software package allowing for the
analysis of strain fields of arbitrary geometry. This software allows
one to model a microscopic strain field with various mathematical methods
(including finite elements) and subsequently verify this model by
comparison with experimental observations in the TEM.

At this moment we would like to know whether anybody would be interested
in this software (SIMCON, i.e. SIMulating CONtrast images). If there are
enough people interested we will probably arrange for a shareware
software release. If you are indeed one of the interested please let me
know by e-mail. To keep track of SIMCON you might check on
http://www.mtm.kuleuven.ac.be/~janssens/simcon.html

Friendly Greetings,

Koen Janssens

=============================================================

Koenraad G. F. Janssens, Dr. Ir.
Katholieke Universiteit Leuven (KUL)
Departement Metaalkunde en Toegepaste Materiaalkunde (MTM)
de Croylaan 2, B-3001 Leuven
Belgium
Tel: +32-16-32.1232
Fax: +32-16-32.1992
Koenraad.Janssens-at-mtm.kuleuven.ac.be




From: MR A HALL, Elektronmikroskopie, X3297 :      HALL-at-scientia.up.ac.za
Date: Mon, 29 Apr 1996 11:56:06 GMT+2
Subject: Buffers,Fixatives,etc

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Microscopists

With regard to all the dicussion on fixatives, buffers,etc; maybe
it's time to suggest two references:

1.Coetzee,J.and van der Merwe, C.F.,Some characteristics of the
buffer
vehicle in gluteraldehyde-based fixatives.Journal of Microscopy,Vol
146 Pt2, May 1987,pp143-155.

2.Coetzee,J. and van der Merwe, C.F.,The influence of processing
protocol on the ultrastructure of bean leave cells.S.African Journal
of Botany,1986 52(2)

Alan Hall
Unit for Electron Microscopy
University of Pretoria
Pretoria.
Tel: +27+012-420 3297
Fax: +27+012-420 3266




From: Vladimir.Oleshko :      oleshko-at-uia.ua.ac.be
Date: Mon, 29 Apr 1996 12:32:36 +0200 (MET DST)
Subject: Apology

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Please accept my apologies for the not intentional mistake. My message
was addressed just to the person posting the job announcement.

Vladimir Oleshko




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Mon, 29 Apr 1996 09:31:52 GMT
Subject: FYI

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} } __________________________________________________________________________
_____
}
} } } } HERE IS SOMETHING THAT WE ALL NEED TO TAKE ACTION ON. PASS THIS ON
} } } } TO YOUR FRIENDS.
} } } }
} } } } } THE NEO-NAZIs are trying to start a news group. Their purpose is to
} } } } get
} } } } } their message of hate out to young people using the Internet. Its
} } } } } originator, Mr. Burdi, is confident they will get the "yes" votes they
} } } } need.
} } } } } He said: "Let me be perfectly blunt and state that we have more than
} } } } enough
} } } } } 'net-nazis' to win this thing hands-down. But every one of you must
} } } } vote
} } } } } 'yes'".
} } } } }
} } } } } Mr. Burdi's confidence is disturbing, so please give this letter the
} } } } widest
} } } } } possible distribution and help us defeat this news group with a your
} } } } "No"
} } } } } vote.
} } } } }
} } } } } News groups are public discussion areas of the Internet and their
} } } } formation
} } } } } requires enough support from the Internet community. EACH AND
} } } } EVERYONE OF
} } } } US
} } } } } HAS ONE VOTE when it comes to creating a new Usenet group. Please
} } } } vote
} } } } } against this news group.
} } } } }
} } } } } In order to vote, E-mail: music-vote-at-sub-rose.com. Your mail message
} } } } should
} } } } } contain only one statement: I vote NO on rec.music.white-power.
} } } } }
} } } } } Vote counting is automatic. Failure to follow these instruction may
} } } } mean
} } } } } that your vote does not get counted. If you do not receive an
} } } } acknowledgment
} } } } } of your vote within three days, contact the vote taker about the
} } } } problem.
} } } } }
} } } } } Please distribute this message.
}
} Dr. Sheldon M. Schuster
} Professor and Program Director
} Box 110580
} University of Florida
} Gainesville, FL 32611-0580
} Phone -- 352-392-8408
} Fax -- 352-392-8598
}
}
}
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 29 Apr 1996 10:23:18 -0500
Subject: Montage FR1 slide

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Slide making machine:
We have a Montage FR1 slide making machine that worked fine for us in older
Macs with systems older than 7.5.2. My secretary told me that the company
went out of business and that the phone was disconnected.

1) Does anyone knows if the compony was bought?
2) Has anyone newer drivers that possible works on newer macs?

I would appreciate any information you can provide and solution. As now, I
contemplate hooking it up to an older mac.

*********************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} ----} Prof. Pathology & Otolaryngology *
*http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html*
*********************************************************************




From: William R Oliver :      oliver-at-ipas4.afip.mil
Date: Mon, 29 Apr 1996 11:40:21 -0400 (EDT)
Subject: Re: FYI

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Why is this on a microscopy newsgroup? If we are all going
to start posting about our particular political hobby horses,
just let me know. I got a million of 'em.


billo





From: jofu-at-enh.nist.gov (Joe Fu)
Date: Mon, 29 Apr 1996 11:45:25 -0400
Subject: Re: FYI

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Message-Id: {199604291704.NAA29393-at-leatherback.nist.gov}
X-Sender: jofu-at-mailserver.nist.gov
X-Mailer: Windows Eudora Version 2.0.3
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Greeting:


Does anyone know the OSRAM Inc. telephone# & address? We need bulb
replacement for our microscope.
Yhank you.

Joseph Fu
NIST Rm.A117 Bldg.220
Gaithersburg, Md. 20899
Tel:301-975-3495
e mail: jofu-at-enh.nist.gov
Fax: 301-869-0822





From: Ann-Fook Yang :      YANGA-at-em.agr.ca
Date: Mon, 29 Apr 1996 12:02:44 -0400
Subject: Frozen osmium

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Message-Id: {s184b0cb.096-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

Charlie was worrying whether frozen osmium tetraoxide was still
good for fixation. Michael and Brett have their osmium stored
frozen.
A colleaque in another lab. liked to store 4% aqueous oxmium in
the freezing compartment. She had had broken ampules on several
occasions. This came to light only after her retirement when her
successor asked me how I stored mine.
I make aqueous solution, seal 0.5ml in each ampule and leave them
in the fume hood; no more osmium fume in the fridge and no black
cooling compartment to worry about.

Ann Fook Yang
Yanga-at-em.agr.ca






From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Mon, 29 Apr 1996 12:38:45 -0400 (EDT)
Subject: Re: Looking for polarizing stereo viewers

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John et al-

Although no longer listed in the catalog, Ted Pella still carries
polarizing glasses for stereo viewing- or at least did a month ago.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************

On Fri, 26 Apr 1996, John Chandler wrote:

} We need to find a supplier of stereo viewers, the cardboard-mounted
} polarizing kind. I've looked in every EM supply house catalog that I have
} and can't find them anymore. I'd appreciate any help in finding these.
} Thanks.
}
} John
} chandler-at-lamar.ColoState.EDU
}
}
}




From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Mon, 29 Apr 1996 09:52:01 GMT
Subject: Re: Question: digital image recording for metallograph

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At 10:16 AM 4/25/96 EDT, you wrote:
}
} Our lab would like to convert our Zeiss Axiomat from film to digital.
} We have no direct experience, and would appreciate any comments or
} experiences that would help us research the best route to take, both
} hardware and software, but particularly the former.
}
} I believe that this topic has been discussed recently, but unfortunately
} I was not concerned until just now! If anyone has saved either their own
} previous postings or those of others, I would appreciate if you would
} email to me directly if you consider it repetitious to repost.



Hi Pat,
I maintain an archive of most of the biological and computer
related questions and responses that appear in this list. If you go to the
www address at the end of this page and click on the "Tips & Tricks Wizard"
you should find a discussion called "Acquiring digital images". Let me know
if you do not have www access and I will be hapy to E-mail it to you.




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/





From: ckblack-at-dow.com
Date: Mon, 29 Apr 1996 11:13:51 -0400
Subject: Re: FYI

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From: ckblack-at-dow.com
Date: Mon, 29 Apr 1996 11:13:51 -0400
Subject: Re: FYI

Contents Retrieved from Microscopy Listserver Archives
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In response to....



} } } } HERE IS SOMETHING THAT WE ALL NEED TO TAKE
ACTION ON. PASS THIS ON
} } } } TO YOUR FRIENDS.
} } } }
} } } } } THE NEO-NAZIs are trying to start a news group.
Their purpose is to
} } } } get
} } } } } their message of hate out to young people using
the Internet.........etc.

------------------------
This forum (microscopy) is for the discussion of
microscopy related material. Political issues and so
forth have NO place here. This is a place where
scientists can discuss science.

We all have opinions concerning issues of censorship
and other such controversial things concerning the
Internet. There are appropriate forums for
addressing your concerns......but not here.


Respectfully.

Cary Black
Dow Chemical

Greg Erdos
Phone: 352-
392-1295
Scientific Director,


ICBR Electron Microscopy Core Lab

218 Carr Hall
Fax: 352-846-
0251
University of Florida E-mail:
gwe-at-biotech.ufl.edu
Gainesville, FL 32611
http://www.biotech.ufl.edu/~emcl/

******************************************************
*

================== RFC 822 Headers ==================



From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Mon, 29 Apr 1996 09:31:52 GMT
Subject: FYI

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From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Mon, 29 Apr 1996 15:16:50 -0600
Subject: Vacuum Pump Repair

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We have a 2 yr old Edwards 2-stage direct drive pump (Model E2M-12, serial
20372) that needs to be repaired (keeps blowing fuses). Anyone know of a
good repair place who might be able to handle this? Many thanks.


#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Mon, 29 Apr 1996 16:57:54 -0500 (CDT)
Subject: Re: your mail

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On Mon, 29 Apr 1996, Joe Fu wrote:

} Greeting:
}
}
} Does anyone know the OSRAM Inc. telephone# & address? We need bulb
} replacement for our microscope.
} Yhank you.
}
} Joseph Fu
} NIST Rm.A117 Bldg.220
} Gaithersburg, Md. 20899
} Tel:301-975-3495
} e mail: jofu-at-enh.nist.gov
} Fax: 301-869-0822


Try:

ESV Inc.
(309)-347-6685

Tom

Thomas Moninger (moninger-at-emiris.iaf.uiowa.edu)
Central Microscopy Research Facility
85 EMRB University of Iowa
Iowa City, IA 52242 319-335-8142 FAX 319-335-8049





From: Beverly E Maleeff
Date: 29 Apr 96 11:41:55 EDT
Subject: May '96 PSM Meeting Notice

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {9604291839.AA1097-at-pho903.sbphrd.com}
To: microscopy {microscopy-at-Sparc5.Microscopy.Com}
{Beverly_E_Maleeff-at-sbphrd.com}

Philadelphia Society for Microscopy Meeting Notice
May 1996


DATE: Thursday, May 9, 1996

PLACE: Laboratory for the Research of Science and Materials
(LRSM) Building, 33rd and Walnut Street, Philadelphia, PA.
Parking is available behind the LRSM Building after 5:00 PM.

TIME: 5:30 Social hour, hosted by our meeting sponsor.

6:30 Dinner

7:30 PM Speaker:

Electron Microscopy in the Study of Copolymers:
Blend Miscibility and Shear-Induced Defects

Dr. Karen I. Winey
Department of Materials Science and Engineering
University of Pennsylvania
Philadelphia, PA

Polymers containing two or more types of monomer units are copolymers.
The different monomer units can be distributed along the polymer chain
in a variety of sequences which produce random, alternating, or block
copolymers. Both the sequence distribution and the relative amounts of
each monomer can produce significant changes in morphological, chemical
and mechanical properties. Two examples will be given from our studies of
copolymers which illustrate the value of electron microscopy.
(1) We have developed a method which combines transmission electron
microscopy, image analysis and mass balance to construct quantitative ternary
phase diagrams. (2) Shear-induced defects, reminiscent of kink bands,
have been imaged using low voltage scanning electron microscopy.
The use of electron microscopy as a tool in these studies will be discussed.


DINNER:

COST: Members $12.00 Student members $6.00 Non-members $15.00

MENU: Barbequed chicken breast
Barbequed beef ribs
Hamburgers with all the trimmings
Red bliss potato salad
Pasta salad with fresh vegetables

Brownies
Fresh fruit

Reservations will be taken by Ms. Pat Overend at the University of
Pennsylvania, 215/898-8337. Deadline for reservations will be Friday,
May 3. If you have any questions regarding the meeting please feel free to
contact
Rollin Lakis at 215/898-2013 or lakis-at-sol1.lrsm.upenn.edu.
Cancellations must be received by Ms. Overend no later than 5:00 PM,
May 3, 1996.





From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Mon, 29 Apr 1996 16:06:51 -0700
Subject: For Sale or Trade

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199604292306.QAA19547-at-cats.ucsc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

These items are offered for sale or trade, I need a wheel for tripod polishing:

1. Polaron Series 6000 Coating System .

2. Kevex thin window, horizontal detector for JEOL SEG stage, detector
only, no electronics.

3. Link Be window, horizontal detector for JEOL SEG stage, Link PP and
bias unit, Dapple X-mate and MicroPlus system.

E-mail for details, each one needs more explanation if you are interested.


Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
FAX (408) 429-0146
jmkrupp-at-cats.ucsc.edu






From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Sun, 28 Apr 1996 23:51:51 -0400
Subject: Technicial Positions Open

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
Message-Id: {v03006601ada9ed196746-at-[141.213.21.13]}
Mime-Version: 1.0
Content-Type: text/enriched; charset="us-ascii"
Content-Transfer-Encoding: quoted-printable

Please pass this information to anyone who is interested.


{bold} {fontfamily} {param} Times {/param} {bigger} {bigger} {bigger} TECHNICAL
SUPPORT OPPORTUNITIES {/bigger}


The University of
Michigan {/bigger} {/bigger} {/fontfamily} {/bold} {fontfamily} {param} Times {/para=
m} {bigger}


{bold} {bigger}

Department of Materials Science and Engineering {/bigger} {/bold} =20


Due to recent and pending retirements, three technical support
positions are open. The department seeks to hire a technical support
team for the research and teaching needs of the department. The
positions that are to be filled are outlined as follows:


{bold} {bigger} Engineer II/III, T-96-1172 {/bigger} {/bold}

Responsibilities include:

Maintenance, service and repair of instruments and research equipment
including electronics, vacuum systems, computer-based systems,
mechanical testing equipment. This includes a number of scanning &
transmission electron microscopes, an Auger system and X-ray
photoelectron spectrometer. Assist with the maintenance and service of=20
X-ray diffraction systems, including a computer controlled Rigaku
system. Installation and networking of computers, interfacing computers
to research instrumentation, installation of data acquisition packages
(e.g. LabVIEW) and programming data acquisition modules for such
packages. Review and improve the safety program of the department.=20
Minimum requirement: B.S. in engineering or equivalent, plus three
years of relevant experience.


{bold} {bigger} Instrument Analyst I/II, T-96-1171 {/bigger} {/bold}

Responsibilities include:

Maintenance and service of two scanning electron microscopes (one scope
is a field emission instrument) and two=20

X-ray diffraction systems, including a computer controlled Rigaku
system. Operate and train students on proper use of above mentioned
instruments. Operate and maintain departmental multimedia facilities
(presentation computers, VCRs, TVs, TV cameras, CD-ROM drives, etc.).
Assist with review and improvement of departmental safety program.=20
Minimum requirement: B.S. in science, engineering, or equivalent,
plus three years of relevant experience.


{bold} {bigger} Computer Systems Specialist I/II,
T-96-1176 {/bigger} {/bold}

Responsibilities include:

Assist in the planning, operation and maintenance of the hardware and
software (network and stand alone) for departmental computer systems.=20
Provide technical advice to faculty, support staff and graduate
students. Coordinate the implementation of problem resolutions for
system hardware, software and network attachments and other operational
needs; arrange for hardware repair; maintain a schedule of system
back-ups and recovery procedures; load, test and implement upgrades to
existing and/or new applications; provide user training on upgraded or
new applications. Evaluation and acquisition of software packages.=20
Installation and networking of computers, interfacing computers to
research instrumentation, installation of data acquisition packages
(e.g. LabVIEW) and programming data acquisition modules for such
packages. Operate and maintain departmental multimedia facilities
(presentation computers, VCRs, TVs, TV cameras, CD-ROM drives, etc.).=20
Assist with review and improvement of departmental safety program.=20
Minimum requirement: B.S. in science or engineering, plus two years of
relevant experience.



Interested individuals should submit 2 copies of resume to: Employment
Services, The University of Michigan, G300 Wolverine Tower, 3003 S.
State Street, Ann Arbor, MI 48109-1281. Reference the Job # listed
next to the job title above. A non-discriminatory, affirmative action
employer.

{bigger}








{/bigger} {/bigger} {/fontfamily}

John Mansfield

North Campus Electron Microbeam Analysis Laboratory

417 SRB, University of Michigan

2455 Hayward, Ann Arbor MI 48109-2143 =20

Phone: (313)936-3352 FAX (313)936-3352

Email: jfmjfm-at-engin.umich.edu

URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html





From: slakmon-at-scottscientific.com (P. Slakmon)
Date: Mon, 29 Apr 1996 15:39:45 -0400 (EDT)
Subject: Conference & Workshop Announcement

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S C O T T S C I E N T I F I C


On the behalf of The International Society of Molecular Morphology

Would like to take the opportunity to announce the

FOURTH INTERNATIONAL CONFERENCE & WORKSHOP
on
MOLECULAR MORPHOLOGY

June 3-4, 1996 - Conference
June 5 -6, 1996 - Workshop
in

Montreal, Canada

CONFERENCE FEATURING
Advances in Principles, Techniques and
Applications in Research and Diagnosis of:

- In Situ PCR
- In Situ Hybridization
- Immunohistochemistry
- Immunogold-Silver Staining
- Immunogold Electron Microscopy
- Microwave Immunohistochemistry
- Atomic Force Probe Microscopy
- Confocal Microscopy
- Antigen Retrieval
- Image Analysis

Call for Abstract Submission
DEADLINE: APRIL 15, 1996

The abstract should be typed single space on white paper within 9 x 7 inch
(23 x 18 cm) typed space. Total of two pages per abstract. Photographs and
references may be included. Please follow the style of CELL VISION, in
which the Proceedings will be published. Abstracts should be submitted in
duplicate.

THREE HANDS-ON WORKSHOPS (at the laboratories of Dept. of Anatomy,
University of Montreal)
1 In Situ PCR and In Situ Hybridization
2 Immunogold EM and Immunogold-Silver Staining
3 Microwave Fixation, Antigen Retrieval and Immunohistochemistry

June 2, 1996 (Sunday), 6.30-9.30 pm:
An optional preparation lecture on "Molecular Biology for the Uninitiated"

ORGANIZING COMMITTEE
CO-CHAIRMEN
Jiang Gu, M.D., Ph.D.
Deborah Research Institute
Browns Mills, New Jersey, USA

Moise Bendayan, Ph.D.
University of Montreal
Montreal, Canada

MEMBERS
Virginia Anderson, M.D.
Health Science Center at Brooklyn
State University of New York
Brooklyn, New York, USA

Gerhard Hacker, Ph.D.
Institute of Pathology
General Hospital, University of Salzburg
Salzburg, Austria

Lawrence DeBault, Ph.D.
Oklahoma University Health Center
Oklahoma City, Ok, USA

Shahla Masood, M.D.
University of Florida Health Science Center
Jackonsville, Florida, USA

Robert Day, Ph.D.
University of Montreal
Montreal, Canada

David Kersten, M.D.
Ealing
London, UK

----------------------------------------------------------------------------
--------
ADVANCED REGISTRATION FORM
(please print and use photocopies for additional forms)

NAME ________________________________

PHONE _________________ FAX __________________

ADDRESS __________________________________________

_________________________________________

CHOICE OF WORKSHOP (circle one): 1 2 3
----------------------------------------------------------------------------
-
ADVANCED REGISTRATION FREE (for one of three):
- For the two-day conference $250 (US)
- For the two-day workshop $350 (US)

Make check payable to CELL VISION. A 15 % discount for members of the
"International Society of Molecular Morphology" and students (with proven
ID). A 15% discount will be reimbursed upon becoming a member of the
society before or at the conference.
Please book the hotel room directly by calling The Best Western Hotel in
Montreal, Canada (800) 361-3000 or Fax (514) 861-4089. You can obtain a
special discounted room rate at $79 (Canadian) per day (rate includes
breakfast) by identifying yourself as a participant of the conference/workshop.
Student dormitory available at University of Montreal (15-minute subway
transporation to conference location, 5 minute walk to workshop location) at
$35 (Canadian) per day by calling (514) 343-6531.

Send abstract, registration form, and registration fee to:
CELL VISION-JOURNAL OF ANALYTICAL MORPHOLOGY,
EDITORIAL OFFICE, DEBORAH RESEARCH INSTITUTE
1 Trenton Road, Browns Mills
NJ 08015-1799, USA
Phone: (609) 735-0477
Fax: (609) 735-0478


For further information please direct your inquiries by email to:
morphology-at-scottscientific.com
_______________________________________________
SCOTT SCIENTIFIC
P.O. Box 66552 Station Cavendish,
Montreal, Quebec, H4W 3J6, Canada

Telephone: 514-485-2309 Fax: 514-485-9931
Voice Mail: 514-888-6509

WWW Site: http://www.ScottScientific.com

E-Mail: slakmon-at-scottscientific.com
info-at-scottscientific.com
sales-at-scottscientific.com
admin-at-scottscientific.com
_________________________________________________


_______________________________________________
SCOTT SCIENTIFIC
P.O. Box 66552 Station Cavendish
Montreal, Quebec, H4W 3J6, Canada

Telephone: 514-485-2309 Fax: 514-485-9931
Voice Mail: 514-888-6509

WWW Site: http://www.scottscientific.com

E-Mail: info-at-scottscientific.com
sales-at-scottscientific.com
admin-at-scottscientific.com
links-at-scottscientific.com
_______________________________________________





From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Mon, 29 Apr 1996 22:19:15 -0500
Subject: From the SysOp... Stick to Microscopy & Microanalysis!!!

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199604300315.WAA05275-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

G'day Colleagues...

A Reminder: This forum should not to be used to post
non-Microscopy/Microanalysis
related announcements, requests, and/or informational items. There are
sufficient
number of public forums in which these issues may be freely and openly debated
and discussed to your heart's desire.

Please refrain from using the Microscopy ListServers for those purposes,
however, noble you may believe your motivations are....

There is no need for the ListServer community to echo this, as enough
bandwidth has
already been wasted, and enough mailboxes cluttered.

Nestor
Your Friendly Neighborhood SysOp






From: IAN HALLETT :      ihallett-at-MARCCRI.MARC.CRI.NZ
Date: Tue, 30 Apr 1996 17:21:52 GMT+1200
Subject: Observing living and dead cells: LM

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Dear All

A colleague wishes to differentiate between living and dead cells.
Does anyone have a simple and reliable method (or if not simple at
least reliable).

The cells are from plant cell suspension cultures that have been
subjected to various environmental stresses.

Thanks in advance

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660
EMail ihallett-at-hort.cri.nz




From: ZHENQUAN lIU :      zqliu-at-pccms.pku.edu.cn
Date: Tue, 30 Apr 1996 16:47:31 -0600 (CST)
Subject: E-mail Address wanted

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X-Nupop-Charset: English


Hi,

I would like to know the E-mail address of:

Professor E. A. Davis.

His mail address is:
Philosophical Magazine
Department of Physics and Astronomy
University of Leicester
University Road, Leicester LEI 7RH
England

If someone knows, please tell me.

Many thanks!

Zhen Quan Liu
---------------------------------------------------------------------
*Important!!!
ALL TELEPHONE NUMBERS in Beijing will be changed from May 8, 1996
from 7 digitals to 8 digitals!!!
The way of changing is by putting 6 infront of the initial number.
Therefore my tel since then will be
Tel: 6275 1427(office)
6275 3727(home)
Fax: 6275 1615
The codes for China and Beijing will not be changed, they still are:
China 86
Beijing 10
--------------------------------------------------------------------
** Sorry, my E-mail software cannot show me the address of
coming mails. Please tell me your E-mail address within
the text.
---------------------------------------------------------------------
Zhen Quan Liu (Ph.D) Tel:(86) 10 275 1427(Office)
Physics Building (86) 10 275 3727(Home)
EM Lab. Email: zqliu-at-pku.edu.cn
Physics Building Email(home) wl-at-ibmstone.pku.edu.cn
Peking University Fax (office): (86) 10 275 1615
Beijing 100871, China




From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Tue, 30 Apr 1996 08:07:25 -0500
Subject: Re: OSRAM Bulbs

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Message-Id: {v021305a1adabc0833cb7-at-[199.77.235.102]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Joe:
Another source is Gray Supply Company. 800-238-2244. They have a wide range of projection of microscope bulbs and will send a catalogue.

--
David Rothbard
Institute of Paper Science and Technology






From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 30 Apr 1996 07:06:40 -0500
Subject: Montage-got it-thanks

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Message-ID: {n1381274340.4479-at-msmail.tmc.tulane.edu}

I already have the information needed and is enclosed below for anyone else
may need it.

The company was bought by the employees. Their new name is Montage
Graphics, the address is 2730 Scott Boulevard, Santa Clara CA 95050. The
phone is Sales - 800-416-4166, Email sales-at-montagegraphics.com, Technical
support 408-654-0684, Tech email tech-at-montagegraphics.com

Mei Lie Wong
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
email wong-at-msg.ucsf.edu




From: rnessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Tue, 30 Apr 1996 09:21:52 -0500 (CDT)
Subject: Re: Vacuum Pump Repair

Contents Retrieved from Microscopy Listserver Archives
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On Mon, 29 Apr 1996, John. J. Bozzola wrote:

} We have a 2 yr old Edwards 2-stage direct drive pump (Model E2M-12, serial
} 20372) that needs to be repaired (keeps blowing fuses). Anyone know of a
} good repair place who might be able to handle this? Many thanks.
}
}
} #############################################################################
} John J. Bozzola, Ph.D., Director
} Center for Electron Microscopy
} Southern Illinois University
} Carbondale, IL 62901-4402
} U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} #############################################################################
}
We had a Balzers rotary pump rebuilt by Atlantic Vacuum Repair
-at-201-623-1115. They did a great job, and I would recommend that you give them a
call. They are at 96 Harper St., Newark, NJ. 07114. I'm one satisfied customer.


Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu






From: Stefan Andreatta :      Stefan.Andreatta-at-uibk.ac.at
Date: Tue, 30 Apr 1996 16:29:02 +0100
Subject: Re: Observing living and dead cells: LM

Contents Retrieved from Microscopy Listserver Archives
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Dear Ian

I have not tried differentiating between living and dead eucaryotic
cells, but maybe it would be a good idea to contact Molecular Probes
Europe BV. They are selling a number of live/dead fluorescence
staining kits for both procaryotic and eucaryotic cells.

(unfortunately they are not going to pay me for posting their address)
Molecular Probes Europe BV, 2333 AA Leiden, The Netherlands
Phone: +31-71-233378; Fax: +31-71-233419

Stefan


* Stefan Andreatta
* Institute of Zoology and Limnology
* University of Innsbruck; Austria







From: Elinor Solit :      cambrex-at-world.std.com
Date: Tue, 30 Apr 1996 10:54:50 -0400 (EDT)
Subject: Re: for microscope manufacturers

Contents Retrieved from Microscopy Listserver Archives
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James,

Try the following companies and people:

Patrick Drexel or Tom Freda at Prior Scientific, 800-877-2234

Irv Toplin at Carl Zeiss, 800-356-1090

Jim Dutkiewctz at Meiji, 800-832-0060.

All these references feature products in The Microscope Book. The Spring
Edition is on press. Would you like a copy?

Please call us if there is anything more we can do to assist.

Elinor Solit, Director of Publications,
The Cambrex Group
800-440-0311

On Fri, 26 Apr 1996, James S MArtin wrote:

} Several months ago I posted a request for microscope manufacturers to
} contact me with information on costs for epi-fluorescence upgrades to
} polarizing light microscopes. The information will be presented at a
} national conference.
}
} So far, I have information only from Olympus. Anybody else like to be
} represented?
}
} James Martin
} Director of Analytical Services and Research
} Williamstown Art Conservation Center
} Williamstown, MA
}




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 30 Apr 1996 12:46:40 -0400
Subject: RE-VacPmpRepair

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Message-ID: {n1381253843.32880-at-mse.engin.umich.edu}

Subject: Time: 7:36 AM
OFFICE MEMO RE:VacPmpRepair Date: 4/30/96

Most manufacturers of vacuum pumps will provide repair and maintenance
services for their pumps. Usually, to save time in getting you back into
operation, they will make an exchange with you, immediately shipping you a
used, but fully reconditioned pump, for the one you send in for repair.
Having the pump serviced by the manufacturer should be about the most
reliable way of ensuring that it receives proper attention.
The phone number of the Edwards service unit is 716-695-6354

The number of the service





From: hua-at-junction.ucsb.edu (Wu Xuehua)
Date: Tue, 30 Apr 1996 11:13:40 -0800
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199604301814.LAA25533-at-surface.ucsb.edu}

unsubscribe please
Xuehua Wu
Materials Department
University of california, Santa Barbara
CA 93106
Tel: 805 893 8523





From: Tony King , INTERNET:tking-at-vacgen.fisons.co.uk
Date: Mon, Apr 29, 1996, 12:40 PM
Subject: Re: Peltier vs adabiatic CPD

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I passed on the inquiry regarding Peltier vs adabiatic coolong of CPDs to Tony
King, Product Specialist at VG Microtech, who manufacture the Polaron range of
critical point dryers which we sell. This is his reply:


RE: Re: Peltier vs adabiatic CPD


One of the main reasons for changing from adibiatic cooling was due to
health and safety, 10% CO2 in air can cause permanent brain damage.
Secondly the cooling of a large vessel suitable for a number of
samples can be slow and difficult to control. If
you use a peltier cooled device the big advantage is that by reversing
the polarity the device acts as a heater with a limited range so it
is safe and efficient for this very application.

The only draw back that we have seen is that in a warm area the
excess heat must be removed by additional cooling. We used air
previously but again this was inefficient so we went to water.

As soon as you add peltier cooling and heating you can run a 3 term
controller and any one that wants to be clever can heat or cool
in precise stages!.

I hope this helps


Regards,

Tony King
Product specialist
VG Microtech/ Polaron range

Tel: +44 (0)1825 746251
Fax: +44 (0)1825 768343

Disclaimer:
The views and opinions expressed are not necessarily
those of Fisons plc or VG Microtech.







From: cxb41-at-po.CWRU.Edu (Christine H. Block)
Date: Tue, 30 Apr 1996 15:13:31 -0400
Subject: Point Source Bulbs

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199604301913.PAA13964-at-roo.INS.CWRU.Edu}

I am looking for a supplier for a point source bulb for
a DURST ENLARGER... any suggestions?

Thanks


--
\\\
(o o)
*Limbic Lady* -------oOO--(_)--OOo-----




From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Tue, 30 Apr 1996 13:31:22 -0500 (EST)
Subject: Re: Vacuum Pump Repair

Contents Retrieved from Microscopy Listserver Archives
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Dear John,
Try letting your maintance shop do it. I was informed by my old job maintance
men that a direct drive pump is good for about 2-3 rebuilds. The belt driven
pumps can take about 8-10 rebuilds.

Best of luck,
Ed Calomeni
Medical College of Ohio
Toledo, OH 43699
emlab-at-opus.moc.edu




From: Eric Steel :      steel-at-enh.nist.gov
Date: Tue, 30 Apr 1996 09:49:45 -0400 (EDT)
Subject: Re: Vacuum Pump Repair

Contents Retrieved from Microscopy Listserver Archives
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Attached is a technical meeting announcement for anyone interested

MAMAS
(Mid-Atlantic Microbeam Analysis Society)
Meeting
at the
National Institute of Standards and Technology
Gaithersburg, MD
on
Thursday, May 9, 1996
10:30 am- 2:15 pm
Lecture Room D, Administration Bldg.

10:30 Coffee and Doughnuts

10:45 Joe Geller, Geller Microanalytical Laboratory, Standards
for the SEM Laboratory, Preparation for ISO-9000

12 noon Lunch

1:15 John Armstrong, NIST, Evaluating and Choosing Standards
for Quantitative Microbeam Analysis

For more information, contact Ryna Marinenko (301)975-3901,
FAX(301)216-1134, email:ryna.marinenko-at-nist.gov


Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov
Microanalysis Research Group Office: 301-975-3902
N.I.S.T. FAX: 301-216-1134
Bldg. 222/Rm A113
Gaithersburg MD 20899





From: cmurphy-at-GGPL.ARSUSDA.GOV
Date: Tue, 30 Apr 1996 16:15:22 -0400
Subject: Many Thanks

Contents Retrieved from Microscopy Listserver Archives
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I would just like to thank all ( and there were many ) scientist that took the time to send me information regarding the frozen 4%
osmium. The information came so quickly that I was able to carry out an experiment with complete confidence of the outcome. By
the way it was unanimous. The OSO4 is O.K. if it has been frozen. Thanks again. Sincerely Charlie Murphy




From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Tue, 30 Apr 1996 10:32:25 -0400 (EDT)
Subject: Re: your mail

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On Mon, 29 Apr 1996, Joe Fu wrote:

} Greeting:
}
}
} Does anyone know the OSRAM Inc. telephone# & address? We need bulb
} replacement for our microscope.
} Yhank you.
}
} Joseph Fu
} NIST Rm.A117 Bldg.220
} Gaithersburg, Md. 20899
} Tel:301-975-3495
} e mail: jofu-at-enh.nist.gov
} Fax: 301-869-0822
}
Joe:
Have you tried Bulbman Inc. in Reno, Nevada? They have had every
type of bulb I've needed. The cost is usually a fraction of what the
manufacturer charges. So far they have been the least expensive of any
other people I've tried. The people are pretty good handling customers
and their needs. Excellant to deal with.
Their number is: 1-800-648-1163
Fax: 1-800-548-6216
One time I had to replace a bulb, the bulb had no recognizable numbers
to give them. I faxed them the type and model of the microscope and they
were able to give me the right bulb.
Hope this helps.

Peace,

Phil

p.s. They have a pretty quick turn around time.




From: John M. Libert :      jlibert-at-cpcug.org
Date: Tue, 30 Apr 1996 17:19:58 -0700
Subject: Re:

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Message-ID: {3186AE2E.1F0D-at-cpcug.org}

Try OPELCO, Inc.
703-471-0080 ext. 544


Joe Fu wrote:
}
} Greeting:
}
} Does anyone know the OSRAM Inc. telephone# & address? We need bulb
} replacement for our microscope.
} Yhank you.
}
} Joseph Fu
} NIST Rm.A117 Bldg.220
} Gaithersburg, Md. 20899
} Tel:301-975-3495
} e mail: jofu-at-enh.nist.gov
} Fax: 301-869-0822




From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 4/30/96 2:42 AM
Subject: Observing living and dead cells: LM

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Dear All

A colleague wishes to differentiate between living and dead cells.
Does anyone have a simple and reliable method (or if not simple at
least reliable).

The cells are from plant cell suspension cultures that have been
subjected to various environmental stresses.

Thanks in advance

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660
EMail ihallett-at-hort.cri.nz





From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Tue, 30 Apr 1996 00:20:08 -0400
Subject: WWW Topical Conference in Minneapolis

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
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Here is a reminder and some clarifying information.


The Microbeam Analysis Society is sponsoring their first Topical
Conference immediately prior to the Microscopy and Microanalysis 96
meeting in Minneapolis this year. The Topical Conference is entitled:


{bold} "Microscopy & Microanalysis Resources on The World Wide Web"

(http://www.microanalysis.org/mas/topicalconf96.html

http://www.microanalysis.org/mas/regtopconf.html)

{/bold}

It will be held in The Minneapolis Convention Center on


SATURDAY THE 10TH of AUGUST 1996.


Note that the ad in Microscopy Today contained a typo stating that the
meeting was to be on the Sunday. The meeting has been placed on
SATURDAY to avoid any conflict with the M&M Tutorials.


The starting time will be 9:00am and the conference will last all day.


The morning will be a series of presentations which will cover the
following:


1. Basic introduction to the Web

2. What is available on the Web generally.

3. What is available on the Web for Microscopy & Microanalysis

4. How to create your own Web Page.


Invited Presenters include:

Greg Meeker - U.S. Geological Survey.

Marc De Graef - Carnegie Mellon University.

Darcy Clark - University of Michigan (formerly University of
Queensland).

John Mansfield - University of Michigan.


The afternoon will be focussed on a hands-on workshop where there will
be a minimum of 15 computer workstations connected to the Internet (via
a T1 line)

for attendees to use. The morning's presenters will be available to
advise attendees on all aspects of accessing and using the Web.


Attendance is FREE to MAS members

Attendance is $35 for Non Members

If you join The Microbeam Analysis Society you may attend free,
membership is $25 per year.


You may register by:

1. Filling out the card that was contained in the last issue of
Microscopy Today

and mailing it in to the address noted on the card.

2. You may register electronically if you are a member. Connect to:

http://www.microanalysis.org/mas/regtopconf.html

3. Non members may complete the form at:

http://www.microanalysis.org/mas/regtopconf.html, print it and mail it
to the address supplied in the form.

4. Members & Nonmembers may send their:

Name

Full address

Business phone

FAX

email address

and an indication of their computer and Web expertise to John
Mansfield

at the address at the end of this message.

Non-Members should enclose a check for $35 (drawn on a US bank) made
out to The Microbeam Analysis Society.



For future updates keep monitoring:

http://www.microanalysis.org/mas/topicalconf96.html

or send mail to John Mansfield (jfmjfm-at-umich.edu)


Thanks.




John Mansfield

North Campus Electron Microbeam Analysis Laboratory

417 SRB, University of Michigan

2455 Hayward, Ann Arbor MI 48109-2143

Phone: (313)936-3352 FAX (313)936-3352

Email: jfmjfm-at-engin.umich.edu

URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html





From: kingsley-at-zephyr.nrlssc.navy.mil (KingsleyMcCrocklin)
Date: 4/30/96
Subject: unsuscribe

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This message was sent by Chameleon
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From: kingsley-at-zephyr.nrlssc.navy.mil (KingsleyMcCrocklin)
Date: 4/30/96
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From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Tue, 30 Apr 1996 13:01:08 -0500
Subject: B&W photos of Cy5

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I have been using Cy5 with great luck on my confocal but now need to take
some B&W photos using my epi-fluorescence rig. I know something like Tri-X
pan would be virtually insensitive to the far red emission of Cy5. Does
anybody have practical experience with Tmax and Cy5 or is there a better
choice of B&W film for this purpose. Thanks.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: !Microscopy-request-at-Sparc5.Microscopy.Com (Robert Kayton,MAC,CROET)
Date: Tue Apr 30 10:54:50 -0400 1996
Subject: Re: for microscope manufacturers

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Sender: kayton-at-ohsu.edu (Robert Kayton)
Message-Version: 2
} To: Microscopy-at-Sparc5.Microscopy.Com

James,

Try the following companies and people:

Patrick Drexel or Tom Freda at Prior Scientific, 800-877-2234

Irv Toplin at Carl Zeiss, 800-356-1090

Jim Dutkiewctz at Meiji, 800-832-0060.

All these references feature products in The Microscope Book. The Spring
Edition is on press. Would you like a copy?

Please call us if there is anything more we can do to assist.

Elinor Solit, Director of Publications,
The Cambrex Group
800-440-0311

On Fri, 26 Apr 1996, James S MArtin wrote:

} Several months ago I posted a request for microscope manufacturers to
} contact me with information on costs for epi-fluorescence upgrades to
} polarizing light microscopes. The information will be presented at a
} national conference.
}
} So far, I have information only from Olympus. Anybody else like to be
} represented?
}
} James Martin
} Director of Analytical Services and Research
} Williamstown Art Conservation Center
} Williamstown, MA
}




From: Ian J. Davies :      davies-at-asuka.nal.go.jp
Date: Thu, 1 May 1986 09:48:05 +0000
Subject: OM - Problem with Olympus SZH

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Message-Id: {9605010047.AA03572-at-asuka.nal.go.jp}
Comments: Authenticated sender is {davies-at-asuka.nal.go.jp}

I work at a research institute in Japan in the field of materials
science. I have experience in TEM and SEM but recently have been
trying to take micrographs using an Olympus SZH optical microscope.
This microscope has a "zoom" lens from nominally 7.5 to 64.

My problem is that this microscope appears to exhibit something
similar to astigmatism even at the lowest magnification. We also have
an Olympus STM5-BD which works perfectly. With the SZH I have tried
to realign the optical system but there is little improvement.
Another department in my institute also has an SZH with exactly the
same problem.

Does anyone know if this problem is common with the SZH
microscope and how it may be corrected.

Please could you send any thoughts and suggestions to me at
DAVIES-at-ASUKA.NAL.GO.JP

Any ideas would be gratefully received. Thank you.

Dr. Ian Davies




From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 30 Apr 1996 17:16:05 -0400 (EDT)
Subject: Re: Peltier vs adabiatic CPD

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} If
} you use a peltier cooled device the big advantage is that by reversing
} the polarity the device acts as a heater with a limited range so it
} is safe and efficient for this very application.
}
} The only draw back that we have seen is that in a warm area the
} excess heat must be removed by additional cooling. We used air
} previously but again this was inefficient so we went to water.
}
} As soon as you add peltier cooling and heating you can run a 3 term
} controller and any one that wants to be clever can heat or cool
} in precise stages!.
}
Dear Tony,
We have a darkroom rinsing tank which is heated/cooled by peltier
devices. One caution with using these devices is that most of the commer-
cial controllers are duty-cycle type, i.e., they turn from full off to full
on for a fraction of the time which depends on the difference between the
set point and the temperature being controlled. This kind of controller is
harmful to peltiers. Our electronics shop designed a controller which puts
out a voltage which is proportional to the set-point-temperature difference,
so it adjusts slowly as the temperature reaches the set point and never has
sudden changes in output. With this kind of controller, the peltiers have
lasted many years without apparent damage.
Yours,
Bill Tivol




From: saz1-at-ix.netcom.com (Steven Zenk )
Date: Tue, 30 Apr 1996 21:10:04 -0700
Subject: Subscription

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Please enter my subscription to your service. Please use the following
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please contact me.

Thanks for your continued efforts to satisfy the needs of the
microscopy consortium.




From: W Owen Saxton :      wos1-at-cus.cam.ac.uk
Date: Wed, 1 May 1996 14:16:31 +0100 (BST)
Subject: W M Stobbs

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Mike died suddenly in Cambridge on Thursday April 25th.

A fund is being set up in his name to assist students at his college:
donations can be sent to The Bursar, Trinity Hall.





From: Giles John E Jr :      giles_john_e_jr-at-space.honeywell.com
Date: 1 May 1996 09:49:37 U
Subject: Sputter Coater Choices

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Message-Id: {199605011351.IAA08840-at-Sparc5.Microscopy.Com}

Hello All,

We are beginning to look at replacements for our aging (8+ yrs) Anatech Hummer
VII. This coater is a bench top unit used for gold coating only although we do
have the carbon coater attachment. This is a standard mechanical pump unit
(non-turbo).

Our work is all materials related (non-biological) and we typically examine
specimens in the 50X to 10,000X region, with an extremely varied selection of
parts and contaminants. The SEM is a LaB6, so I don't believe I need the
ability to deposit chromium or other metals used for high magnification work.

The main features I would look for are bench-top size; relatively quick cycle
times; automatic push-button operation, with the ability to have some manual
control for bleeding in argon to help dry samples; and a cost in the $8-10K
region. The ability to effectively carbon coat samples would be nice if it
worked well. The current Hummer unit has problems when trying to carbon coat
filter pads.

I would like to solicit input from the group on their recommendations for
purchase of a new coater and any experiences with reliability issues of the
various units that are available.

Thanks in advance for any help.

John Giles
Senior Materials Engineer
Honeywell Space Systems




From: Louisa.Howard-at-Dartmouth.EDU (Louisa Howard)
Date: 01 May 96 09:10:36 EDT
Subject: Thanks for info. on long term fixation

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Message-id: {14165111-at-prancer.Dartmouth.EDU}

Thanks to everyone who shared their experience with long term fixation. Before
any field work is started, we will use HEPES and PIPES buffers with the GTA
fixative and compare the results after storing mammalian brain tissue for -at-
1month in both GTA/buffer and buffer alone. I don't know when this will occur,
as it depends on grant applications, coordination with other labs, etc. If
anyone is interested in what we discover with this comparison, please send me
an e-mail message and I will contact you with the results.
thanks again,
Louisa Howard
EM Facility-Remsen 240
Dartmouth College
Hanover, H.N. 03755

Louisa.Howard-at-dartmouth.edu





From: Crisnilson-at-aol.com
Date: Wed, 1 May 1996 12:53:07 -0400
Subject: Subscription

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Please subscribe me to your list server at my e-mail address:

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From: SERVICE-at-KEVEX.COM
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Please subscribe me to your list server. Use my e-mail address of: stevez-at-kevex.com

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From: wut-at-ctrvax.Vanderbilt.Edu
Date: Wed, 01 May 1996 13:18:53 -0600
Subject: sub

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From: saz1-at-ix.netcom.com (Steven Zenk )
Date: Wed, 1 May 1996 14:13:25 -0700
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From: Diane Montpetit :      montpetitd-at-em.agr.ca
Date: Wed, 01 May 1996 13:17:22 -0400
Subject: enzymes, tem level, atpase, succinate dehydrogenase

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Message-Id: {s1877346.054-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

Good day everyone,

I will be working with animal muscle (pork) at the tem level looking
for 2 enzymatic activities; atpase and succinate dehydrogenase.

I would like to have references or recipes dealing with traditional enzyme
cytochemistry or immunocytochemistry.

Thank you,

Diane Montpetit
Food Research Center
agriculture canada
St-Hyacinthe, Quebec
Canada






From: klivi-at-jhu.edu (Kenneth JT Livi)
Date: Wed, 01 May 1996 16:27:24 -0400 (EDT)
Subject: longevity of direct-drive mechanical pumps

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Dear MSA,

While following the thread on pump rebuilds, I wondered if some of you have
extended experience with the ages of roughing pumps? What are the ages
typically achieved with the usual routine maintenance?
I recently had two Edwards pumps fail on me. The cause seemed to be
excessive water build-up in the oil chamber that caused rusting. These
pumps are used only to prepump film desiccaters. I also found that some of
the Al parts were corroded, possibly from calcium sulfate dust from the
desiccant. I run the pumps with the ballast somewhat open, but it seems
that the exhausted water condenses in the hoses we use to vent the fumes
and drips back down into the pump housing.
I am wondering if my pumps are prematurely failing or if they're reaching a
ripe age (one is about 15 years old).

Ciao for now,
Ken

Kenneth JT Livi
Department of Earth and Planetary Sciences
34th and Charles Streets
The Johns Hopkins University
Baltimore, Maryland 21218

klivi-at-jhu.edu (e-mail)






From: Sandra F. Zane :      sfzane-at-unccvm.uncc.edu
Date: Wed, 01 May 1996 14:06:17 -0500
Subject: hierarchy

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Hello fellow microscopists,
On the 17th of April I sent out a questionnaire regarding the
administrative hierarchy of your labs. There was a very healthy response to
this questionnaire and I have attempted to summarize the responses for those
of you who might be interested. The one area which I haven't yet been able
to deal with yet is the various responsibilities. So, what I will do is
post what I have and when I find time to deal with the rest, I will try to
post that,too.
I really do appreciate all your comments. They have been very helpful.
For those who asked, I am making an attempt to revitalize our lab.
We have a TEM and all ancillary equipment. And we have access to a SEM and
have in our lab a sputter coater, a critical point dryer and a vacuum
evaporator. As the EM work has dropped off in recent years, I have proposed
that we form an integrated microscopy lab. One of the first additions I
would like to see is a confocal microscope. There are also a couple of
fluorescence microscopes in the department as well as some video equipment
which might, (or not) become a part of the integrated facility. However, I
am the only person employed in the lab at the present time. I do everything
from washing glassware to producing the micrographs of the EM projects,
ordering, scheduling, etc. Any students or others (some from outside the
university community) needing assistance come to me. So I thought that
before I bit off more than I could chew, I would ask you how you ran things.
Each and every response which I received was helpful in one way or
another and I really do appreciate your taking time to help me out.
Following is the portion of the summary which I have completed.
There were 25 responses and out of those 25:

16 have directors and 9 have coordinators (lab managers or lab
supervisors)

Of the 16 directors, 12 are faculty and 4 are staff employees.

Of the 12 faculty directors, 10 have 12 month contracts.
The 4 staff directors also have 12 month contracts.

Of the 9 coordinators their supervisors ranged from:

1. senior staff committee, 2. nominated academic staff, 3.
dean of COAS, 4. dept. head, 5. faculty committee.

All have 12 month contracts. All are staff except 1 who is
a bit unclear.

Again, thank you very much.

Best wishes,
Sandra
Sandra F. Zane, EM Tech. sfzane-at-email.uncc.edu
Dept. of Biology, UNCC Ph.(704)547-4051
9201 University City Blvd. Fax (704)547-3128
Charlotte, NC 28223





From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 5/1/96 12:04 PM
Subject: Sputter Coater Choices

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Hello All,

We are beginning to look at replacements for our aging (8+ yrs) Anatech Hummer
VII. This coater is a bench top unit used for gold coating only although we do
have the carbon coater attachment. This is a standard mechanical pump unit
(non-turbo).

Our work is all materials related (non-biological) and we typically examine
specimens in the 50X to 10,000X region, with an extremely varied selection of
parts and contaminants. The SEM is a LaB6, so I don't believe I need the
ability to deposit chromium or other metals used for high magnification work.

The main features I would look for are bench-top size; relatively quick cycle
times; automatic push-button operation, with the ability to have some manual
control for bleeding in argon to help dry samples; and a cost in the $8-10K
region. The ability to effectively carbon coat samples would be nice if it
worked well. The current Hummer unit has problems when trying to carbon coat
filter pads.

I would like to solicit input from the group on their recommendations for
purchase of a new coater and any experiences with reliability issues of the
various units that are available.

Thanks in advance for any help.

John Giles
Senior Materials Engineer
Honeywell Space Systems





From: Karpura V Kommineni :      komminen-at-pilot.msu.edu
Date: Wed, 1 May 1996 20:51:13 -0400 (EDT)
Subject: Unsubscribe

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Message-Id: {199605020051.UAA137860-at-pilot16.cl.msu.edu}

Please unsubscribe .





From: John Millar :      jjmill-at-rmit.edu.au
Date: Thu, 2 May 1996 12:09:27 EST-10
Subject: Re: longevity of direct-drive mechanical pumps

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On Wed, 01 May 1996 16:27:24 -0400 (EDT)
Kenneth JT Livi wrote

} What are the ages
} typically achieved with the usual routine maintenance?

These comments refer to both belt and direct drive pumps. Life is
about 10 - 15 years, and longer on cleaner systems

} I recently had two Edwards pumps fail on me. The cause seemed to be
} excessive water build-up in the oil chamber that caused rusting. These
} pumps are used only to prepump film desiccaters.

Very hard on the pumps and you could expect shortened life, I think

} the Al parts were corroded, possibly from calcium sulfate dust from the
} desiccant. I run the pumps with the ballast somewhat open, but it seems
} that the exhausted water condenses in the hoses we use to vent the fumes
} and drips back down into the pump housing.

We have always used a sump in the exhaust line as close as possible to the
pump itself and it is amazing the amount of fluid which is collected.

} I am wondering if my pumps are prematurely failing or if they're reaching a
} ripe age (one is about 15 years old).

I think that's probably pretty good.
Cheers
jjm
Professor John J. Millar, PhD
Department of Applied Physics and
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 9660 2602 fax 613 9660 5290
email jjmill-at-rmit.edu.au




From: em-at-mediacity.com (Ed Monberg)
Date: Wed, 1 May 1996 23:22:12 -0800
Subject: Re: longevity of direct-drive mechanical pumps

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} I am wondering if my pumps are prematurely failing or if they're reaching a
} ripe age (one is about 15 years old).
}
} Ciao for now,
} Ken


There are several practices applicable to your case:

If the moisture is chronic and the use is intermittent,
motoor oil. It is amazing stuff, and will emulsify the H2O,
loosing only a little VP due to additives.

The heavy duty H2O applications with continuous operation
use a heavier oil weight which causes the pump to run
hotter than 212F.





Regards,



(signed) Ed Monberg {em-at-mediacity.com}

--------------------------------------------------

510-429-1060 Fax 429-1065
LMDC, (Laser Motion Development Co.)
3101 Whipple Road
Union City, CA 94587-1216


For our Most recent Catalogue of "On Hand" EQUIPMENT:
Send empty mail to: {Cat-at-lasermotion.com}


Our web page: http://www.lasermotion.com (Is beginning to take shape!)
Our e-mail: office-at-lasermotion.com

{-------------------------------- Our page width
-----------------------------}






From: Dr. jiechao Jiang :      jiangj-at-papin.hrz.uni-marburg.de
Date: Thu, 2 May 1996 09:44:43 +0000
Subject: unsubscrible

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Message-Id: {9605020740.AA23954-at-HRZ.Uni-Marburg.DE}
Sender: {jiangj-at-papin.hrz.uni-marburg.de}

please unsubscribe
____________________
Dr. Jiechao Jiang
Philipps-Universitaet Marburg
Fachberich Geowissenschaften
Hans-Meerwein-Str.
35043 Marburg

Tel. +49 6421 28-3458
Fax. +49 6421 28-8919
E-Mail: Jiangj-at-papin.hrz.Uni-Marburg.De




From: John M. Libert :      jlibert-at-cpcug.org
Date: Thu, 02 May 1996 05:35:30 -0700
Subject: Re: OM: 3D reconstructions from serial sections

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Message-ID: {3188AC12.21FC-at-cpcug.org}

GeoffA,
For 3D reconstruction as well as digital confocal (i.e., reduction of
out-of-focus haze in optical sectioning) VAYTEK, Inc. has several very
nice software products for PC. Their software is available as well for
Mac and for UNIX systems. VOXBLAST will do your 3D reconstruction. I
understand that VAYTEK has developed a new product for "rubber sheeting"
sections for a better fit, though I've not seen this. MicroTome and
HazeBuster are products that employ digital deconvolution methods to
sharpen each image in a series of frames acquired at incremental planes
of focus in a thick section. The deconvolution algorithms are much more
sophisticated than conventional edge enhancement filters found most image
processing software. The VAYTEK products are available as "stand-alone"
windows programs or as "plug-ins" to image analysis packages such as
Media Cybernetics Image Pro Plus. You can get more information about
the products at VAYTEK's web site
http://www.vaytek.com

Good luck!

John Libert
OPELCO
OPtical ELements COrporation


GeoffA wrote:
}
} Hi all from Sydney where, I'm sad to say, Winter is on the way and it's
} a wet sub-20 degrees. And I note that all those conferences in
} Australia have affected Nestor when he opens a message with "G'day"!
} Will he trade that hat of his for a Croc Dundee model?
}
} One of our colleagues would like to do 3D reconstructions from serial
} histological sections on a PC. Over the years he has tried this a few
} times with drawing tablets and various early graphics software but
} always given up because it has been ultimately easier to do it with
} pencil and paper. He now has a better setup with 'scope, CCD and speedy
} Pentium but we still have the perennial problem of registering
} successive sections.
}
} Can anybody tell me of any DOS/Windows (even Unix) image analysis
} software (or dedicated reconstruction software) which allows for
} independent registration of slices within a stack and maybe
} 'rubber-sheeting' as well?
}
} I know that Prism allows this but it is Mac-based. Elsewhere in our
} Museum we have Mac versions of both Dicer and VoxelView (around 1990
} versions) but neither of these lets us move sections.
}
} Any help gratefully received,
}
} Geoff Avern
} Microscopy Laboratories
} Australian Museum
} Sydney, Australia.




From: Kari Kinnunen :      Kari.Kinnunen-at-gsf.fi
Date: Thu, 2 May 1996 15:42:11 +0300
Subject: unsubscribe

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Message-Id: {199605021157.HAA16992-at-post-ofc02.srv.cis.pitt.edu}
To: confocal {confocal-at-ubvm.cc.buffalo.edu}

unsubscribe






From: Peter Torok :      pt201-at-cus.cam.ac.uk
Date: Thu, 2 May 1996 15:37:59 +0100 (BST)
Subject: subscribe

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Please subscribe

********************************************************
** Dr Peter Torok **
** University of Cambridge **
** Multi-Imaging Centre **
** Downing Street, Cambridge CB2 3DY, UK **
** email: pt201-at-cam.ac.uk (private) **
** mic-at-lists.cam.ac.uk (work) **
** phone: +44 1223 333774 **
** fax: +44 1223 333786 **
********************************************************





From: John Grazul :      GRAZUL-at-BIOLOGY.RUTGERS.EDU
Date: 2 May 96 11:39:25 EDT
Subject: freeze etch consumables...alternate sources?

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To: microscopy-at-sparc5.Microscopy.com

Freeze Etchers,

Besides Tech no Trade (who has yet to send me a new phone number,
catalog, address) where might I get platinum pellets, hollow
carbon, and filaments for my BAF 301? I have talked to a company
that will help me out with the carbon and platinum, but the tungston
filaments are another story, or maybe techno trade can get me their
number so I can throw a couple of hundred bucks their way.



John Grazul
Rutgers University
Electron Microscope Facility




From: Steven W. Miller :      73150.2217-at-CompuServe.COM
Date: 02 May 96 11:15:53 EDT
Subject: DURST Parts, Point Source Bulbs

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The point source bulbs for the L1200 are called PULAM, they are custom made and
cost $128 each. These are 24V 100Watt bulbs.

For older units like S45 and S138, S183, there is an adapter for the Edison base
(usual lamp socket) to adapt to a bayonet bulb. These are not as good but the
only thing that fits the physical constraints in the old units. The adapter is
$60 and the bulbs are $12. These are 20Volt 100 Watt bulbs.

We offer an educational discount of 8%.

Please make sure on the old enlargers that you have removed the heat filter
normally used only with the 200-300 watt diffuse bulbs, they are almost surely
fogged with age and totally eliminate the benefits of point source. The normal
voltage for the transformer in the old units is 110 volts, you must make a
voltage adapter.

For other questions please feel free to call us.

Steve Miller
Integrated Microsytems, Inc. (An Authorized Durst Dealer)
P.O.Box 1074
Park Ridge, IL 60068
Phone 800-388-8801
Fax 847-696-2541






From: knoff-at-lipovx.lbl.gov (Laura Knoff)
Date: Thu, 2 May 1996 09:41:36 -0800
Subject: May 9 Nor Cal Micro Meeting

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Message-Id: {199605021637.LAA01177-at-Sparc5.Microscopy.Com}
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Please make your dinner reservations today (May 2) or tomorrow for this event.

} Northern CA Society for Microscopy (NCSM) Meeting Announcement
}
} Where: Roche Biochemicals (formerly Syntex), Gallery
} Conference Center Bldg A-2
} 3401 Hillview Ave, Palo Alto CA
}
} When: Thursday, May 9, 1994, from 2:30 to 8:45 PM
}
}
} 2:30 -3:00 Registration
} Speakers
} 3:00-3:45 Hank W. Bass, UC Berkeley, Molecular and Cellular
} Biology, "Analysis of Meiosis using 3-D
} Deconvolution Light Microscopy". 4:00-4:45 David Blake,
} NASA Ames Research Center, Space Sciences Division
} "Electron Microscopy of Astrophysical Ice"
} 5:00 - 6:30 Social Hour and Local Society A filiate meeting
} sponsored by JEOL Instruments
} 6:30-7:30 Dinner. Choices: Marinated Stripped Loin with sundried
} tomatoes, Lemon chicken with caper sauce, or roasted
} vegetable lasagna,
} each with garlic mashed potatoes, summer squash medley,
} mixed green salad, dinner rolls, and dessert.
} 7:45 - 8:45 Paul Carpenter, Manager Analytical and Geology, Cal
} Institute of Technology, "XRay and Optical
} Methods as Applied to the Study of Gemstones."
}
} Please help us to plan accurately. Make your reservation before Friday MAY
} 3rd
} Fax reservation(s) with meal preference to Laura Knoff at (510) 486-4750.
} E-Mail address = KNOFF-at-LIPOVX.LBL.GOV
} Phone = (510) 486-4088, if leaving a message please speak slowly and clearly.
} Price: $17 for Loin, $14 for Chicken, $13 for Lasagna,$8 for student
} members.
} Avoid standing in line by pre-paying. Send your check, payable to NCSM,
} to NCSM c/o Laura Knoff
} LawrenceBerkeley National Lab
} Bldg 1, Room 264
} 1 Cyclotron Rd
} Berkeley, CA 94720
Sorry about the format (or lack of it).






From: LYNNE (617) 386-1446 :      GARONEL-at-cliffy.polaroid.com
Date: Thu, 02 May 1996 14:57:22 -0400 (EDT)
Subject: JEOL TEMSCAN 100CX FOR SALE

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TEMSCAN WITH 120KV, PURCHASED 7/82, UNDER SERVICE CONTRACT, ASID W. FREE LENS,
SCAN ROTAT. AND TILT, EXT. WAVE FORM MONITOR, MAGNETIC SPEC. POLE PIECE, SEG, 4 HOLDERS INCLUD. OXFORD COLD, LIQ. N2 BAFFLE, GATAN CAMERA (NEGOTIABLE)
ASKING $15K, WILL COST $12-15k TO MOVE AND SET-UP. MORE QUESTIONS:
GARONEL-at-POLAROID.COM




From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Thu, 2 May 1996 16:00:22 -0400 (EDT)
Subject: RE: freeze etch consumables...alternate sources?

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In message 2 May 96 11:39:25 EDT,
"John Grazul" {GRAZUL-at-BIOLOGY.RUTGERS.EDU} writes:

} Freeze Etchers,
}
} Besides Tech no Trade (who has yet to send me a new phone number,
} catalog, address) where might I get platinum pellets, hollow
} carbon, and filaments for my BAF 301? I have talked to a company
} that will help me out with the carbon and platinum, but the tungston
} filaments are another story, or maybe techno trade can get me their
} number so I can throw a couple of hundred bucks their way.
}
}
}
} John Grazul
} Rutgers University
} Electron Microscope Facility
}
----------
Try contacting Albrecht (Albi) Auwater or Johnny Hagen at:

TECNO TRADE INTERNATIONAL
7 perimeter Road
Manchester, NH 03103-3343
Tel (603) 622-5011 Fax (603) 622-5211

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology &
Director, Cornell Integrated Microscopy Center
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: lizard-at-okway.okstate.edu (Ginger Baker)
Date: Thu, 2 May 1996 16:24:48 -0500
Subject: Ultramicrotomes

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Mime-Version: 1.0

Hello,

I am writing for a colleague of mine who is interested in puchasing
an updated ultramicrotome. Does anyone have a list of companies that
sell microtomes? The two I know are RMC and Leica. Are there more?
Also, does anyone have a preference for one microtome over another?

Please send responses to Terry Colberg at colberg-at-okway.okstate.edu.

Thank you in advance,

Ginger R. Baker
EM Lab Manager
Department of Anatomy, Pathology, and Pharmacology
250 Veterinary Medicine
Oklahoma State University
Stillwater, OK 74078
(405) 744-6765
FAX: (405) 744-5275
Email: lizard-at-okway.okstate.edu




From: rnessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Thu, 2 May 1996 13:52:16 -0500 (CDT)
Subject: Re: freeze etch consumables...alternate sources?

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On 2 May 1996, John Grazul wrote:

} Freeze Etchers,
}
} Besides Tech no Trade (who has yet to send me a new phone number,
} catalog, address) where might I get platinum pellets, hollow
} carbon, and filaments for my BAF 301? I have talked to a company
} that will help me out with the carbon and platinum, but the tungston
} filaments are another story, or maybe techno trade can get me their
} number so I can throw a couple of hundred bucks their way.
}
}
}
} John Grazul
} Rutgers University
} Electron Microscope Facility
John,
I just got my order from them. It came quite quickly, actually. The
address from the to of the invoice reads:
Technotrade International
7 Perimeter Road
Manchester, NH 03103-3343
Tel (603)622-5011
Fax (603)622-5211

As far as part numbers, I think they use the old Balzers catalog item numbers.
The tungsten cathodes I ordered were item number BU020 23T. Sorry, I didn't
order any Pt pellets or hollow carbon. The Balzers numbers for these items are:
Pt inserts, 1.5X2mm #BD481 505, hollow carbon=#BD484 055. Give them a call and
see if they cross-reference. I threw a couple hundred bucks their way.


Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu





From: mgb-at-Nucleus.ansto.gov.au (Mark Blackford)
Date: Fri, 3 May 1996 10:51:07 +1000
Subject: Re: TEM X-ray analysis artefacts

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Message-Id: {199605030051.KAA16045-at-atom.ansto.gov.au}
X-Sender: mgb-at-nucleus.ansto.gov.au
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on Wed, 24 Apr 96 Malcolm Haswell asked:

} MY QUESTIONS ARE:
} 1. Does anyone out there have similar problems with this configuration of
} TEM?
} 2. Is this a problem with most TEMs (small specimen chamber etc)?
} 3. Has anyone any suggestions about how to minimise or remove this problem?
}

Malcolm,

I noticed your posting last week concerning spectral artefacts from
your Hitachi H7000 TEM and H7110 STEM (STEM & SEM); Link QX2000 EDS x-ray
analysis system. Since I have not seen any replies on the Listserver I
thought it was time to pass on my own experiences.

Our microscopes are JEOL 2000 fx and 2000 fxII TEM's with high
take-off angle detectors (~72=B0). The detectors are old Tracor Northern
units with new Link ISIS microanalysers. Both are almost exclusively
operated at 200KV in TEM mode. We frequently observe Fe-K and compton
scattered Mo-K lines in spectra. The Iron is presumable fluoresced in the
specimen area by the compton scattered Mo-k x-rays which are generated in
the condenser system.

Replacing the selectable and/or fixed condenser apertures by very
thick Mo or Pt apertures should eliminate the compton scattered Mo-k x-rays
(or so I'm told, I'm still trying to get part numbers for my microscopes).
Removing the condenser aperture strip is not a good idea since the
electrons will be sprayed over a much larger area of the sample/specimen
chamber than just the area you are analysing (hence the larger Fe peak).

If you try the thick aperture as I've suggested, or any other
fixes, please let us know how successful it is. Regards,

Mark Blackford
TEM Group
Materials Division, Ansto
PMB 1,
Menai, N.S.W.
Australia
2234
Phone (02) 717 3027
=46ax (02) 543 7179






From: saz1-at-ix.netcom.com (Steven Zenk )
Date: Thu, 2 May 1996 22:03:55 -0700
Subject: 3d Reconstruction

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Subscribe Steve Zenk




From: alan.wilson-at-dsto.defence.gov.au (Alan Wilson)
Date: Fri, 3 May 1996 16:35:32 +1000
Subject: Re: TEM X-ray analysis artefacts

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X-Sender: alan.wilson-at-SoMPop.dsto.defence.gov.au
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} I have been using a Hitachi H7000 transmission microscope for several years
} and my x-ray spectra are often affected by background Mo peaks and
} occasionally Fe.
Fe is presumably coming from scattered electrons hitting the pole-pieces or
something else in the region of/below the specimen, but this will depend on
geometry and I do not know the Hitachi.

Is the Mo peak really Mo? Check that it is not slightly lower in energy
i.e. about 17.38keV rather than 17.44keV. If it is then the problem is due
to Compton scattering, probably in the various grahite inserts, of Mo
X-rays. The Mo X-rays will be from thin Mo condensor apertures. This is
elliminated by the use of thick apertures. (See reference AR Wilson and LT
Lambrianidis, J. of Microscopy, vol160, pt1 Oct 1990, pps 1-7.)


} The objective aperture seems to be the biggest contributor of Mo and when I
} removed the aperture strip from the microscope the Mo disappeared but I got
} more Fe (presumably from the holder tip). This would be inconvenient anyway
} because of the machine down time etc.

What is the objective aperture made out of? If Mo then the problem is due
to scattered electrons hitting the aperture (that's what it is for).


alan.wilson-at-dsto.defence.gov.au
Dr Alan Wilson
Senior Research Scientist
Ship Structures and Materials Division
Aeronautical and Maritime Research Laboratory
Defence Science and Technology Organization
506 Lorimer St
Fishermens Bend 3207
Victoria Australia
ph 61 3 9626 7508, fax 61 3 9626 7816 or 61 3 9626 7087






From: MELSEN :      MELSEN-at-MICROBIO.emory.edu
Date: Fri, 3 May 1996 8:38:22 EST
Subject: CRYO NOVA

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Our lab is in need of a Cryo-Nova system as a backup and spare parts source. If anyone has
one in storage or permanantly off-line and is willing to part with it for a minimal amount of
money, please contact me.





From: dwaters-at-api.com (Dean Waters)
Date: Fri, 3 May 1996 12:36:10 -0700
Subject: Position Available

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X-Sender: dwaters-at-api.com
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To: Microscopy-at-Sparc5.Microscopy.Com



Customer Support Engineer
*************************
Due to continued growth in its biomedical division, Applied Precision, Inc.
is presently seeking a Customer Support Engineer to work for the DeltaVision
product line. DeltaVision is an optical sectioning, deconvolution microscope
for high resolution, fluorescence imaging in 5-dimensions.

We are seeking a person with a technical background, experience in optical
microscopy and research instrumentation, and experience with or aptitude for
using SGI/Unix-based computer systems. The person must have excellent
communication skills. Responsibilities include customer support, system
installation and service, and support of conferences and workshops.
Extensive travel is required.

Ideal candidates are interested in learning about and helping others to
understand and use new technology. You must be comfortable working in a
team-based environment, and be able to work independently.

This is a very good opportunity to contribute to a rapidly growing product
that makes a significant contribution to the field of microscopy.

For more information about this opportunity, send your cover letter and
resume to:

Applied Precision, Inc.
8505 SE 68th St.
Mercer Island, WA 98005
Attn: Pete Williams

email: info-at-api.com

Resumes will be held in confidence.


________________________________________________________________________
Dean Waters
DeltaVision Systems
Applied Precision, Inc.
8505 SE 68th Street
Mercer Island WA 98040

dwaters-at-api.com
(206) 236-0704 x4408
(206) 232-4184 FAX





From: cxb41-at-po.CWRU.Edu (Christine H. Block)
Date: Fri, 3 May 1996 18:49:06 -0400
Subject: Ultramicrotomes

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Message-Id: {199605032249.SAA03753-at-roo.INS.CWRU.Edu}

Are there people out there with mt7000 RMC ultramicrotomes?
If so, are you happy with them?

We have 2 and have had so many problems, that I am about
to pull all my hair out with these.
They range from electronic to mechanical.

Any informatio would be helpful.

THANKS!

Christine H. Block, Ph.D.

--
_____
|\___\
|| |
\|___| go TRIBE, rattle SEATTLE, make 'em QUAKE!




From: dwaters-at-api.com (Dean Waters)
Date: Fri, 3 May 1996 17:01:03 -0700
Subject: Position Available

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X-Sender: dwaters-at-api.com
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To: Microscopy-at-aaem.amc.anl.gov



Alas, I typed my home zip code rather than our company zip code in the
address noted below. Please forgive the double posting, and note the correction.

Customer Support Engineer
*************************
Due to continued growth in its biomedical division, Applied Precision, Inc.
is presently seeking a Customer Support Engineer to work for the DeltaVision
product line. DeltaVision is an optical sectioning, deconvolution microscope
for high resolution, fluorescence imaging in 5-dimensions.

We are seeking a person with a technical background, experience in optical
microscopy and research instrumentation, and experience with or aptitude for
using SGI/Unix-based computer systems. The person must have excellent
communication skills. Responsibilities include customer support, system
installation and service, and support of conferences and workshops.
Extensive travel is required.

Ideal candidates are interested in learning about and helping others to
understand and use new technology. You must be comfortable working in a
team-based environment, and be able to work independently.

This is a very good opportunity to contribute to a rapidly growing product
that makes a significant contribution to the field of microscopy.

For more information about this opportunity, send your cover letter and
resume to:

Applied Precision, Inc.
8505 SE 68th St.
Mercer Island, WA 98040
Attn: Pete Williams

email: info-at-api.com

Resumes will be held in confidence.


________________________________________________________________________
Dean Waters
DeltaVision Systems
Applied Precision, Inc.
8505 SE 68th Street
Mercer Island WA 98040

dwaters-at-api.com
(206) 236-0704 x4408
(206) 232-4184 FAX





From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Fri, 3 May 1996 21:16:38 -0800
Subject: Re: TEM X-ray analysis artefacts

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X-Sender: mager-at-pop.unixg.ubc.ca
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} I have been using a Hitachi H7000 transmission microscope for several years
} and my x-ray spectra are often affected by background Mo peaks and
} occasionally Fe.

Dear Malcolm,
I have a Hitachi H-800 200 kV STEM with an Ortec EDX system at high
take-off angle: 68 degrees. Unlike you, I cannot run the EDX with the
objective aperture in place, since the back scattered electrons and X-rays
from the Mo objective aperture flood and "kill" the EDX system. However, I
can run the EDX system with the sample untilted. Generally, I find the
spectrum is very clean and free of any artifacts, but if I count for a long
time I get a small Fe and Co peak, presumably from the inside of the sample
chamber. Can you remove the Mo objective aperture, or swing it out of the
way? Apparently, the main column modification required for optimum EDX
operation in a STEM is shielding on the moveable condenser aperture. My
system has a pure graphite fixed condenser aperture and a thick Mo moveable
aperture with lead shielding above and graphite covered aluminum below.
This is the modification required for an effective EDX STEM system. Do you
know if this system is in your TEM? The only other help I know of is to use
carbon-coated nylon grids or BE grids to reduce scattering from the grid
bars.
The only other solution I have heard of is to cover the objective
pole-piece with a shaped insert of beryllium or possibly painting it with
carbon DAG. The secret is to figure out where the electron and scattered
X-rays are entering your detector or alternately what your detector is
"looking" at, and shield it in some way.
I hope this is some help.
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: em-at-mediacity.com (Ed Monberg)
Date: Sun, 5 May 1996 12:03:21 -0800
Subject: Re: OM - Are you tilted ?

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} My problem is that this microscope appears to exhibit something
} similar to astigmatism even at the lowest magnification. We also have

} Please could you send any thoughts and suggestions to me at
} DAVIES-at-ASUKA.NAL.GO.JP
}
} Any ideas would be gratefully received. Thank you.
}
} Dr. Ian Davies

Dear Ian,

If the plane of the object being viewed is not at 90 degrees to the
axis of the microscope, it can offer a view similar that of
an astigmatic system.



Regards,



(signed) Ed Monberg {em-at-mediacity.com}

--------------------------------------------------

510-429-1060 Fax 429-1065
LMDC, (Laser Motion Development Co.)
3101 Whipple Road
Union City, CA 94587-1216


For our Most recent Catalogue of "On Hand" EQUIPMENT:
Send empty mail to: {Cat-at-lasermotion.com}


Our web page: http://www.lasermotion.com (Is beginning to take shape!)
Our e-mail: office-at-lasermotion.com

{-------------------------------- Our page width
-----------------------------}






From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Mon, 6 May 1996 08:46:07 -0500
Subject: re: RMC ultramicrotomes

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I purchased an RMC MT7000 almost two years ago and have been very satisfied with its performance. I think we needed service once. I have been impressed with their technical and service support.

No commercial endorsement should be implied.

--
David Rothbard
Institute of Paper Science and Technology






From: Rolf Odselius :      Rolf.Odselius-at-emu.lu.se
Date: Mon, 6 May 1996 16:18:36 +0100
Subject: Re: critical point dryer problems

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We have had this problem several times. The diaphragm in the outlet
is probably clogged with some foreign substance. This diaphragm is
found inside a nut, holding the outlet pipe on rear side of the
CPD030. It is easily removed. Inspect the small hole of the
diaphragm in a stero microscope.

It has happened, especially on the earlier instruments in the series,
that small Teflon pieces from the valves obstruct the outlet. This
can happen because the valves are placed after the filter.

Good luck!

Rolf Odselius

} We have a Bal-Tec CPD030. It has been used very little. This is
} because we cannot get it to drain. It might take all night for the
} alcohol-CO2 mixture to empty. The instructions seem very
} straigt-forward. We fill partially with 100% ethanol (with or
} without a sample), cool to 8 degrees C, let in the carbon dioxide
} (yes, the tank is the kind with the dip tube), then press the
} medium out button. At first it seems to empty, then gets very slow.
} Has anyone had a similar problem? The valves seem to work as I
} hear a loud click when pressing buttons. The filters are clean.
} Any other ideas? thanks.
_________________________________________________________________
Rolf Odselius, PhD |E-mail: Rolf.Odselius-at-emu.lu.se
Electron Microscopy Unit |Phone: +46 46 171075 office
University Hospital | +46 46 293692 home
S-221 85 Lund, Sweden |Pager: +46 740 288992 Minicall
http://www.emu.lu.se/ |Fax: +46 46 172975
http://www.ldc.lu.se/~scandem/ |Cellular: +46 70 5581085 GSM/SMS




From: Bjorn_Bergsten-at-pei.philips.com (Bjorn Bergsten)
Date: Mon, 6 May 1996 10:46:38 -0400
Subject: Subscription

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Please subscribe me to your list server at my e-mail address;

bjorn_bergsten-at-pei.philips.com

Thank you,

Bjorn Bergsten
EDAX International




From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Mon, 6 May 1996 11:44:29 -0500
Subject: Re: RMC ultramicrotomes

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In message {v02130504adb3b23e3508-at-[199.77.235.102]} David Rothbard writes:
} I purchased an RMC MT7000 almost two years ago and have been very satisfied
} with its performance. I think we needed service once. I have been impressed
} with their technical and service support.

We bought a new MT-7000 about 4 years ago. Over all its been a good performing
ultramicrotome for us in a multiple user lab with many users. Initially we had a
problem with the fine mechanical advance slipping on the knife stage. Eventually
the stage was replaced under warranty with a new one. Some accessory parts on
the original order, such as water trough filling device, blockface viewing
mirror, service manual, were slow in coming(1-2 years), but finally arrived and
it is good solid equipment. (As an aside, thats not the first time I've bought
"ghostware": "Yes it has a part number and yes its described in the glossy
brochure and yes you paid for it and yes.....we're working on it".)

We like the wide range of controls and flexibility and ease with which it can be
operated and with which new sectioners can learn to use it.

This view is not a commercial endorsement, just the experience and opinion of
one ultramicrotome supervisor and user.

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: Raymond F Egerton :      egerton-at-phys.ualberta.ca
Date: Mon, 6 May 1996 11:03:42 -0600 (MDT)
Subject: Faculty Position

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The Department of Physics, University of Alberta, Edmonton, Canada
is advertising a tenure-track Assistant Professorship in Experimental
Condensed Matter Physics and Materials Science, starting 1 Jan 1997.

Preferred areas of research are: scanning-probe microscopy,
nanostructures, thin films, surface physics and high-temperature
superconductors.

For further information, please contact egerton-at-phys.ualberta.ca
or dtlunty-at-phys.ualberta.ca






From: Dennis B. Barr :      dennbarr-at-eastman.com
Date: Mon, 6 May 1996 14:00:41 -0400
Subject: Re: Question: digital image recording for metallograph

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At 12:56 PM 4/25/96, Pat Kingman wrote:
}
} Our lab would like to convert our Zeiss Axiomat from film to digital.
} We have no direct experience, and would appreciate any comments or
} experiences that would help us research the best route to take, both
} hardware and software, but particularly the former.
}
} I believe that this topic has been discussed recently, but unfortunately
} I was not concerned until just now! If anyone has saved either their own
} previous postings or those of others, I would appreciate if you would
} email to me directly if you consider it repetitious to repost.
}


Perhaps some info about my experiences would help.

I am a microscopist in an industrial research lab. We have electron and
optical microscopes in many varieties. Our optical microscopes are made by
Zeiss, Leitz, Wild, B&L and Nikon and include stereos, low-mag macros, and
conventional research-grade microscopes. I set up analog video imaging on
our optical optical microscopes seven years ago.

Seeing a need to keep copies of images, I switched from analog video imaging
to digital imaging on our optical microscopes last year. A "standard"
microscope workstation consisted of the following:

1. Microscope
2. Optical coupler for video camera
3. Video camera
4. Computer
5. Image capture board
6. Image storage device
7. Printer

The Optical couplers were from Diagnostic Instruments, ~$300.

The video camera was the Hitachi CV-20, a 3-chip camera with C-mount. It
has great image quality and light sensitivity! It cost around $4500.

The computer was a 486 PC. I would recommend a Pentium, the fastest you can
afford. The 486 works OK, but is a little slower.

The image capture board was a TARGA+ from Truevision. This is an older
board, but it was matched to our Kodak 450GL printers. I would recommend a
newer board so as to be more compatible with currently available software.
Boards run ~$2000.

The image storage device was to be a recordable CD-ROM, however because of
the large number of images we expected to generate and the need to have
computer network access to those images, we installed an optical disk
"jukebox" capable of storing 40gB of images from both the optical and
electron microscopes. All microscope workstations are on a network which
accesses this "jukebox". Recordable CD-ROMs run ~$1,000.

The printer was a Kodak 450GL dye-sub printer. Kodak has since discontinued
the manufacture of these printers. A real shame because they are really
quite good. Also, it met our need for a ~4X5 inch print. Sony and Hitachi
make similar printers. I don't know about their pricing. When we need
higher quality images on an 8 1/2 X 11 inch format we use a Kodak 8600PS
dye-sublimation printer. We use it for both electron and optical
micrographs. It cost ~$9000.

Everyone who uses this system is quite happy with the ease of use and
quality of image output. The only thing we lack at this point is a good
database system for our images. There are several available, but we have
not found one that meets all of our specialized needs. As a last resort, we
plan to develop our own if we haven't found one in a few months.

I hope this helps you and anyone wanting to get into digital imaging.

Dr. Dennis B. Barr
Eastman Chemical Company
Microscopy and Morphology Research Laboratory
P.O. Box 1972
Kingsport, TN 37662-5150

Voice: 423/229-2188
E-mail: dennbarr-at-eastman.com
FAX: 423/229-4558






From: Colin Veitch :      C.Veitch-at-geel.dwt.csiro.au
Date: Mon, 6 May 1996 22:21:26 -0500
Subject: Measuring the beam current on a JEOL 2010

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Hi all,

We have a JEOL 2010 TEM and need to measure the beam current either just
prior to or just after EDXS analysis, without the desaturation of the
filament (LaB6) or removal of the sample holder. We currently use a Gatan
cryo/analytical holder which has a Faraday cup in on it but the position of
the cup is beyond the limit of travel of the goniometer. We also have a
normal (non-analytical) JEOL sample holder.

The TEM doesn't have a backscatter detector so we can't use that method. We
do have a Gatan imaging filter/EELS system and could measure the beam
current on the drift tube of this but the setup for EDXS is different from
that of EELS and also we would prefer to measure the current at the sample!

Does anyone have any ideas as to how we could measure the beam current at
the sample with our current equipment as we don't want to have to purchase
yet another holder?

Many thanks in advance

Colin Veitch.






From: Bernd Feja :      feja-at-ubaclu.unibas.ch
Date: Tue, 07 May 1996 10:31:07 +0200 (MET)
Subject: Image analysis

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I have digital micrographs of latex spheres and need to calculate a
radial intensity profile (grayvalues vs. radius). Does anybody know
if for this a macro for NIH Image is available?

Thanks, Bernd


------------------------------------------------------------
| Bernhard Feja | |
| Biozentrum, MSB | Tel. +41 / 61 / 267 2073 |
| Klingelbergstrasse 70 | |
| CH-4056 Basel | Fax +41 / 61 / 267 2259 |
| Switzerland | |
------------------------------------------------------------







From: webred-at-postnet.se
Date: Mon, 6 May 1996 17:06:04 +0200 (MET DST)
Subject: Vdlkommen till torget

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Hej, titta p=E5 detta! Snyggt. Och det b=E4sta av allt, portotarifferna
finns ocks=E5!

Rolf

------- Forwarded Message Follows -------

Grattis!

Du dr anmdld som smygtittare och har gjort dig fvrtjdnt av adressen
http://www.torget.se
I morgon blir denna adress officiell, du fer adressen redan idag!

Vdlkommen till Torget,

Redaktionen

P.S. Passa pe att anmdla dig som medlem pe Torget redan nu! D.S.

_________________________________________________________________
Rolf Odselius, PhD |E-mail: Rolf.Odselius-at-emu.lu.se
Electron Microscopy Unit |Phone: +46 46 171075 office
University Hospital | +46 46 293692 home
S-221 85 Lund, Sweden |Pager: +46 740 288992 Minicall
http://www.emu.lu.se/ |Fax: +46 46 172975
http://www.ldc.lu.se/~scandem/ |Cellular: +46 70 5581085 GSM/SMS




From: philf-at-NEWTON.UMSL.EDU (Phil Fraundorf)
Date: Tue, 07 May 1996 05:23:22 -0500
Subject: Re: Image analysis (of radial profiles)

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Bernd Feja wrote:

} I have digital micrographs of latex spheres and need to calculate a
} radial intensity profile (grayvalues vs. radius). Does anybody know
} if for this a macro for NIH Image is available?

This is a special case of a useful calculation sometimes referred to
as "azimuthal averaging". It is also useful in "reciprocal space",
for example in improving signal to noise in powder diffraction
profiles, or when locating image contrast transfer function zeros.
We use a verb in Semper6 for this purpose, which also offers a way
to locate the center of a circle (or your sphere) given 3 points
on its perimeter. For diffraction patterns, we've put together a
Semper macro for statistically refining the center determination with
more than one triplet of points on one or more "rings" around the
center, and calculating an uncertainty in that determination to boot.

If there is interest in the macro let me know. I am not sure where
to find out about the availability of the Semper6 language itself
these days, however.

Cheers. /philf :)





(~ -at-)
//\/\/\/\--oo0-(_)-0oo--}
// P.Fraundorf Phys&Astr/CME 3145165044 philf-at-newton.umsl.edu
\\ U.Missouri-St.Louis MO 63121 http://www.umsl.edu/~fraundor
\\/\/\/\/\/\/\/\/-----------------}





From: keller-at-boulder.nist.gov (Bob Keller)
Date: Tue, 7 May 1996 07:48:07 -0600
Subject: radial intensity dist.

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Bernd,

Within NIH Image, there is a macro file called "Plotting macros". Load
this file into NIH Image under the special menu, and use the one called
"Radial Intensity Distribution..." It's just that. You pick the point on
your image from which to do a plot (use the select lines tool to choose the
point), the number of lines to do profiles along around the point, and
finally the radius of the lines. It will plot a composite histogram of all
the profiles.

Bob Keller
NIST
Materials Reliability Division






From: METENGR-at-aol.com
Date: Tue, 7 May 1996 12:56:17 -0400
Subject: prickly gold grids

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Hello out there:

Does anyone know the process used to produce/make Prickly Gold Grids. We are
interested in producing our own but don't know how to go about it.

So if anyone can share their method or methods or any insights, we would be
grateful. Thanks,

Laura L. Helm
Asst. to the President
M.E. Taylor Engineering, Inc.
Phone: 301-975-9798 * FAX: 301-975-9653
e-mail: Metengr-at-aol.com






From: klivi-at-jhu.edu (Kenneth JT Livi)
Date: Tue, 07 May 1996 15:17:58 -0400 (EDT)
Subject: Supplier of film measuring device

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Dear All,

A colleague of mine is setting up her own microscopy prep lab and has asked
where we purchased our angular and linear film measuring device. Ours comes
from the Charles Supper Co. which doesn't seem to exist any more. The
microscopy supply catalogues do not have a similar device. What we want is
a device that would sit on top of a light table. We don't want to use a
lupe to measure angles. Does anyone know of a supplier for such a tool.
Ciao for now,
Ken

Kenneth JT Livi
Department of Earth and Planetary Sciences
34th and Charles Streets
The Johns Hopkins University
Baltimore, Maryland 21218

klivi-at-jhu.edu (e-mail)






From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Tue, 7 May 1996 18:20:28 -0500
Subject: "wet-coating" for SEM

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Does anyone have an address for a USA distributor of the Sn/Pd colloidal
coating solution made by:

Neyco s.a.
Paris, France

Thanks!
Phil

&&& Illigitimi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&
Philip Oshel
Center for Electron Microscopy
University of Illinois
(217)244-3145
oshel-at-ux1.cso.uiuc.edu







From: Stephen Edgar :      s.edgar-at-auckland.ac.nz
Date: Wed, 8 May 1996 11:17:37 NZS
Subject: Osmium in "good" buffers

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Hi everyone,

Further to the recent discussion on "good" buffers, has anyone
besides me had problems with osmium made up in PIPES?

I tried using PIPES buffer many years ago after reading the 1973
paper of Salema & Brandao, and got very nice results (with fungal
tissue at that time) but the osmium solution went brown within half
an hour of being made up in PIPES (diluting 2 percent Os in water
to 1 percent with double-strength PIPES). I made up the buffer
several times from the same batch of PIPES powder but always got the
same result. So I went back to cacodylate.

Was it just me? Did I perhaps have a "bad" batch of "good" buffer?



Regards

Stephen Edgar

Electron Microscope Unit, Pathology Department
School of Medicine
University of Auckland
Private Bag 92019
Auckland
New Zealand

email address: s.edgar-at-auckland.ac.nz
Phone : +64-9-3737599 extn 6473 (GMT + 12h)
Fax : +64-9-3737459




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Tue, 7 May 1996 21:50:51, -0500
Subject: SEM preparation

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Message-Id: {199605080150.VAA16092-at-mime2.prodigy.com}
X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc06

-- [ From: Charles A. Garber., Ph.d * EMC.Ver #2.10P ] --

The following was written:
===============================
Does anyone have an address for a USA distributor of the Sn/Pd
colloidal
coating solution made by:

Neyco s.a.
Paris, France

Thanks!
Phil
=========================================
SPI Supplies has been the representative for NEYCO S.A. for North
America. We can supply you with the product.

The technique was developed by French researchers and NEYCO has the
world wide rights (as I understand it) to distribute this product. You
can contact NEYCO directly as follows: {neyco-at-imaginet.fr} .

For your further information, NEYCO has been a long time distributor in
France of products for materials science research. They are the French
distributors, among other firms, of products of SPI Supplies and also
South Bay Technology. The person to contact is Ms. Isabel Richardt.
They are located in a Paris.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.com

####################################
WWW: http://www.2spi.com
####################################
======================================================




From: Stefan Andreatta :      Stefan.Andreatta-at-uibk.ac.at
Date: Wed, 8 May 1996 12:23:19 +0100
Subject: mic

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cc
___________________________________________________________

Stefan Andreatta
Institute of Zoology and Limnology, University of Innsbruck
Technikerstrasse 25, 6020 Innsbruck, Austria
phone: fax:
{Stefan.Andreatta-at-uibk.ac.at}




From: Joe D Geller :      geller-at-world.std.com
Date: Wed, 8 May 1996 08:42:38 -0400 (EDT)
Subject: Re: Supplier of film measuring device

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Charles Supper company can be found at:

Tech Circle
Natick, MA
617 237-2995

Joe Geller
Geller MicroAnalytical Lab
508 887-7000
jg-at-gellermicro.com




From: kna101-at-utdallas.edu
Date: Wed, 8 May 1996 08:50:52 -0500 (CDT)
Subject: Re: Bulbs

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Damian,
Try Microlites Scientific, ph. 1-800-263-8902. Talk to Frank Nasser.
They are located in Canada, but I had no trouble getting bulbs sent to me
in Dallas, TX. I went to them when my local sources didn't have what I
needed and they were very helpful. Good Luck.

Karen

On Tue, 7 May 1996, Neuberger, Damian wrote:

}
} Hi All:
}
} Can anyone recommend a source of microscope illuminator bulbs? I'm looking
} for OSRAM 64625, 12V/100W and Olympus 6-8V5ATB-1, 6V/30W, a DDL 150W 20V
} bulb for our fiber optic light source and similar types of bulbs. Thanks
} for any suggestions you may have.
}
} Damian Neuberger
} neuberd-at-baxter.com
}




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Wed, 8 May 1996 09:27:18 -0500
Subject: Re: Osmium in "good" buffers

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Message-Id: {v01540b00adb65f021da6-at-[128.206.15.189]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I used osmium in HEPES buffer for years although I have recently switched
to using unbuffered osmium in water with no noticeable difference.

} Hi everyone,
}
} Further to the recent discussion on "good" buffers, has anyone
} besides me had problems with osmium made up in PIPES?
}
} I tried using PIPES buffer many years ago after reading the 1973
} paper of Salema & Brandao, and got very nice results (with fungal
} tissue at that time) but the osmium solution went brown within half
} an hour of being made up in PIPES (diluting 2 percent Os in water
} to 1 percent with double-strength PIPES). I made up the buffer
} several times from the same batch of PIPES powder but always got the
} same result. So I went back to cacodylate.
}
} Was it just me? Did I perhaps have a "bad" batch of "good" buffer?
}
}
}
} Regards
}
} Stephen Edgar
}
} Electron Microscope Unit, Pathology Department
} School of Medicine
} University of Auckland
} Private Bag 92019
} Auckland
} New Zealand
}
} email address: s.edgar-at-auckland.ac.nz
} Phone : +64-9-3737599 extn 6473 (GMT + 12h)
} Fax : +64-9-3737459

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: John, Martha,Maryand Sarah McCann :      mccanns-at-tiac.net
Date: Wed, 08 May 1996 10:21:10 -0500
Subject: LM Short Course Announcement

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Message-ID: {3190BBE6.4AB1-at-tiac.net}

} } SHORT COURSE ANNOUNCEMENT
} } -------------------------------------------------
} } FUNDAMENTALS AND APPLICATIONS OF LIGHT MICROSCOPY
} } -------------------------------------------------
} } JUNE 16-21, 1996, Burlington Vermont
} }
} } www.microscopyed.com

Experienced microscopy problem solvers will teach a 5-day hands-on
course on achieving the maximum information from light microscopy. The
emphasis of the course will be to provide hands-on experience, and the
background for interpretation of images.
} }
The course will cover the principals of light microscopy, contrast
techniques for the microscope, adjustments of the microscope for
optimum contrast and resolution, interpretation of images in terms of
light-matter interactions and image recording.
} }
A full range of reflected and transmitted light microscopes, as well
as contrast equipment, will be provided for use by the students.
Students are encouraged to bring their own samples.
} }
This course will be applicable to all disciplines using light
microscopy. Although ideal for beginners, it is designed as an
advanced workshop, with the opportunity to concentrate on light
microscopy in a relaxed environment.
____________________________________________________________
} }
Faculty:
Philip C. Robinson, ret. Staffordshire University, UK, Dept of
Ceramics, author of the RMS Microscopy Series book, Applied Polarized
Light Microscopy. Robert Hoffman, inventor of the Hoffman Modulation
Contrast system, Mary. McCann, course organizer, Robert Janes,
Metropolitan (London) Forensic Science Laboratory, and Dennis O'Leary,
MicroOptical Methods.
Vermont Optecs, research instrument specialists, will supply a variety
of microscope equipment for the course.
} }
For further information:www.microscopyed.com
} }
For course brochure and registration, contact Mary McCann, course
organizer, e-mail: mccanns-at-tiac.net telephone 617-484-7865
fax: 617-484-2490




From: John W Heckman :      heckman-at-pilot.msu.edu
Date: Wed, 8 May 1996 10:23:08 -0400 (EDT)
Subject: Re: Osmium in "good" buffers

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Message-Id: {199605081423.KAA134087-at-pilot09.cl.msu.edu}


Stephen et al,

Over the years I've tried the Good buffers for various (mostly botanical)
specimens. OsO4 will react with (at least) the piperazine containing ones,
likely in competition with what you're trying to fix in the first place. I
generally use OsO4 in water, unbuffered, with no apparent drawbacks.

A second consideration is that the buffers that Good's group were working on
were for physiological experiments, and one of their criteria was
that the buffer should not cross biological membranes. I'm not sure this is
good (so to speak) for ultrastructural preservation, if there is an appreciable
lag between introduction of the first fixation cocktail and the loss of
membrane selectivity.

For a good basic discussion of buffers, in general, I've always liked
D.E.Gueffroy's booklet called (not supprisingly) Buffers, from Calbiochem
Biochemicals, Behring Diagnostics division of Americanm Hoechst. Also for Good
buffers there is Good, et al. 1966. Biochem 5:467.

I use cacodylate buffers mostly now, too.

Cheers,
John Heckman

TEM supervisor
Center for Electron Optics
Michigan State University
.}
} Hi everyone,
}
} Further to the recent discussion on "good" buffers, has anyone
} besides me had problems with osmium made up in PIPES?
}
} I tried using PIPES buffer many years ago after reading the 1973
} paper of Salema & Brandao, and got very nice results (with fungal
} tissue at that time) but the osmium solution went brown within half
} an hour of being made up in PIPES (diluting 2 percent Os in water
} to 1 percent with double-strength PIPES). I made up the buffer
} several times from the same batch of PIPES powder but always got the
} same result. So I went back to cacodylate.
}
} Was it just me? Did I perhaps have a "bad" batch of "good" buffer?
}
}
}
} Regards
}
} Stephen Edgar
}
} Electron Microscope Unit, Pathology Department
} School of Medicine
} University of Auckland
} Private Bag 92019
} Auckland
} New Zealand
}
} email address: s.edgar-at-auckland.ac.nz
} Phone : +64-9-3737599 extn 6473 (GMT + 12h)
} Fax : +64-9-3737459
}





From: CHAFFEYN :      NIGEL.CHAFFEY-at-bbsrc.ac.uk
Date: Wed, 8 May 1996 17:32:29 +0000
Subject: Bubbly methacrylate

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Via: uk.ac.bbsrc; Wed, 8 May 1996 17:31:37 +0100
X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; Relayed;
Wed, 8 May 1996 17:33:16 +0000
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Wed, 8 May 1996 17:32:29 +0000
Receipt Notification Requested) (IPM Return Requested)

Dear Fellow microscopists,

Can anyone help me solve a problem I have with UV-cured
butyl-methyl-methacrylate? I have been using this resin for many months,
deresining with acetone and immunolocalizing tubulin and F-actin in
secondary vascular tissues of horse chestnut. Great, the system works well,
apart from one small niggle: I have been unable to prevent formation of
bubbles/vortices within the the resin as it polymerizes. Usually this is not a
problem because the tissue blocks are dense enough to stay on the bottom of
the cylindrical embedding capsules that I use. However, it is a problem with
young tissue and root tips - which are not as dense - and often the tissue
floats up to the surface of the resin on the bubbles and is generally unusable.
Does anyone have a cure for this phenomenon? I have tried placing the
resin-filled capsules under vacuum for 30 min prior to curing but not avoided
bubble formation...
Any suggestions will be most welcome. Thank you,

Nigel Chaffey

-------------------------------------------------------------------------------
Internet: nigel.chaffey-at-bbsrc.ac.uk IACR-LARS, Dept. of Agricultural
X400:G=nigel; S=chaffey; O=bbsrc; P=uk; C=GB Sciences, University of Bristol,
Tel: +44 (0)1275-392181 ext:230 Long Ashton Research Station,
Fax: +44 (0)1275-394281 Long Ashton, Bristol, BS18 9AF
-------------------------------------------------------------------------------




From: David Shortt :      David.Shortt-at-is.dal.ca
Date: Wed, 8 May 1996 09:46:09 -0300
Subject: Parts for Zeiss EM109

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Am looking for replacement parts for the ion getter pump on the Zeiss EM109,
in particular, the getter grids. The pump is a Leybold-Heraeus IZ80.





From: Rolf Odselius :      Rolf.Odselius-at-emu.lu.se
Date: Wed, 8 May 1996 12:13:29 +0100
Subject: Apology!

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Message-Id: {9605082012.AA61400-at-acs1.acs.ucalgary.ca}

An internal message within our department has of a presently unknown
technical reason been sent to the Microscopy List. A posting I did
send to the list did not turn up though. We must have had some kind
of cross-over in our mailserver. Sorry folks!

Maybee e-mail isn=B4t as safe as it is said to be...
And thanks for all kind remarks about our "exotic" language :-)

Rolf
_________________________________________________________________
Rolf Odselius, PhD |E-mail: Rolf.Odselius-at-emu.lu.se
Electron Microscopy Unit |Phone: +46 46 171075 office
University Hospital | +46 46 293692 home
S-221 85 Lund, Sweden |Pager: +46 740 288992 Minicall
http://www.emu.lu.se/ |Fax: +46 46 172975
http://www.ldc.lu.se/~scandem/ |Cellular: +46 70 5581085 GSM/SMS




From: jfb :      jfb-at-uidaho.edu
Date: Wed, 08 May 1996 15:44:21 -0700
Subject: subscription

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Please subscribe




From: m.stevens-at-qut.edu.au (Mark Stevens)
Date: Thu, 09 May 1996 09:00:49 +1000 (EST)
Subject: subscribe

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subscribe microscopy m.stevens-at-qut.edu.au
----------------
Mark Stevens
Ext. 5037
Analytical EM Facility,
Faculty of Science, QUT





From: Dirk.Voeste-at-rz.ruhr-uni-bochum.de
Date: Thu, 9 May 1996 13:19:34 +0000
Subject: unsubscribe

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Please unsubscribe - dirk.voeste-at-rz.ruhr-uni-bochum.de




From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Thu, 9 May 1996 9:20:20 -0400
Subject: Re: Knife Sharpness Revisited

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Message-ID: {32DF913101F70300-at-mhs.unc.edu}
In-Reply-To: {37D3913101F70300}

Bob,
I've been examining tissues of all types for a good many years and there
are usually several options (preperation wise) that are dependent on what
you want to see.

Need a couple more specifics ie: type of tissue and what you want to look
at. Given these I may well be able to poit you in the right direction
.
regards,
bob

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Thu, 9 May 1996 09:39:34 -0600
Subject: Film Recorders/Slide Makers

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Message-Id: {199605091452.HAA14859-at-holonet.net}

A friend is interested in purchasing a film recorder (slide maker) for
producing high resolution color slides or transparencies. This is a
professional facility where hi res is important and price is a side issue.
Please send suggestions and comments to: jrichard-at-siu.edu

Thank you.

#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: DAVID_GANTT-at-GSVMS2.CC.GASOU.EDU (DAVID G. GANTT)
Date: Thu, 9 May 1996 11:44:18 -0400
Subject: unsubsribe

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Message-Id: {v01530501adb7c30fc310-at-[141.165.35.119]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

unsubscribe DAVID_GANTT-at-GSVMS2.CC.GASOU.EDU

Dr. David G. Gantt Phone: 1-912-681-5964
Dept. of Biology Fax: 1-912-681-0845
Landrum Box 8042 e-mail: david_gantt-at-gsvms2.cc.gasou.edu
Georgia Southern University
Statesboro, Georgia 30460-8042








From: alan_devenish-at-pei.philips.com (Alan Devenish)
Date: Thu, 9 May 1996 10:35:44 -0400
Subject: Subscription

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Mime-Version: 1.0


Please add me to your subsciber list at my email address
Thank you
Alan Devenish




From: DDKJoe-at-aol.com
Date: Thu, 9 May 1996 10:30:53 -0400
Subject: Re: Bubbly methacrylate

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The problem you describe sounds very similar to what is often encountered
with methylmethacrylate embedding. The bubbles usually form right at the end
of the polymerization reaction when the medium is so viscous that they cannot
escape to the surface.

Most often the bubbles are from boiling monomer which nucleate on your
tissue. You need to address ways of lowering the temperature. I would
suggest:
- decreasing the volume of monomer polymerized
- lowering the concentration of initiator or catalyst
- placing the molds in a volume of water to act as a heat sink
- performing the polymerization under pressure (raising the boiling pt)

Obviously not all of these are practical solutions. Still, you should find a
way of lowering the maximum temperature that the solution achieves.

Good luck,
Joe Tabeling
Delaware Diamond Knives
3825 Lancaster Pike
Wilmington, DE 19805
800-222-5143
FAX:302-999-8320
http://www.ddk.com




From: garlasco-at-newsoft.it (Garlasco)
Date: Thu, 9 May 1996 21:10:49 +0200
Subject: LM - polarized - Asbestos

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Dear All,

I use LM with polarized light and contrast liquids (potassium iodo mercurate
in water/glycerol) at 200 and 500 X.
For Chrysotile I use also nitrobenzene or mixtures of cinnammic aldehyde and
benzyl alcohol. I have a Chrysotile standard.
My problem is to manage other asbestos fibers, if present.
How I can see the other asbestos fibers: Crocidolite, Tremolite,
Anthophyllite, Amosite and Actinolite ? I don't have standards with those
fibers.
Some of you can help me ?


Thank you !!

Renzo Garlasco

garlasco-at-newsoft.it





From: Eric-at-Mail.ASD.K12.AK.US (Eric Chapman)
Date: Thu, 9 May 1996 07:56:06 -0900
Subject: Microtome

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Message-Id: {v01540b00adb7d0f2f42b-at-[199.165.105.124]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I have just obtained my dream microscope and and trying to get set up in my
home for a small lab. I need to purchase a microtome. Also, I would like a
line on glassware and slides. I have the VWR Scientific catalog but was
wondering if there are other vendors I might write to for catalogs.

If any of you have phone numbers or addresses I would appreciate it. Also,
if anyone has a used microtome for sale I would love to hear from you.

---------------------------------------------------------------
Eric Chapman - Eagle River Alaska _/_/_/ _/_/_/ _/_/
Hemodialysis Patient _/ _/ _/ _/ _/
_/ _/_/ _/
Sic Gorgiamus Alles _/ _/ _/ _/ _/
Subjectamos Nunc _/_/ _/_/_/ _/_/
---------------------------------------------------------------






From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Thu, 9 May 1996 9:53:03 -0400
Subject: Re: Bubbly methacrylate

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Message-ID: {34DF913101F70300-at-mhs.unc.edu}
In-Reply-To: {31D3913101F70300}

The bubbles are probably being formed during polymerization. I have
seen this happen with Epon-Araldite and GMA However we did not use UV
polymerization. We polymerized at 4o C in a vacuum dessicator and it
eliminated most of the problem. Another trick that I used to use on
tissues that had a tendency to float was to place a small amount of resin
in the capsule, allow it to to partially polyermrize, place the tissue in
the mold and then fill it up with the plastic resinand allow to cure. We
also degassed our resin for at least an hour prior to filling the
capsules.
regards
bob

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: XiaoGuang Ning :      ningx-at-mcmail.cis.mcmaster.ca
Date: Thu, 9 May 1996 18:50:33 -0400 (EDT)
Subject: TEM sample prep of SiC fibers?

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Dear Microscopists:

Could you please tell me how to well prepare for a TEM sample of SiC
fibers in order to observe their cross-sections???

Your help is appreciated very much.

X.G.NING





From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 9 May 1996 15:42:07 -0400 (EDT)
Subject: Re: longevity of direct-drive mechanical pumps

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Dear Ken,

} While following the thread on pump rebuilds, I wondered if some of you have
} extended experience with the ages of roughing pumps? What are the ages
} typically achieved with the usual routine maintenance?

We have several belt-drive pumps which are chugging along quite
happily after 20+ years, but the direct drive ones have somewhat shorter
lives in our experience.

} I recently had two Edwards pumps fail on me. The cause seemed to be
} excessive water build-up in the oil chamber that caused rusting. These
} pumps are used only to prepump film desiccaters.

We also have found problems here. The best pump for this seems
to be a small belt-drive Edwards. We also have a very long path between
the darkroom and the pump, which might help with the water and CaSO4. I'm
afraid that this service is just very hard on pumps.
Yours,
Bill Tivol




From: mrdunlap-at-ucdavis.edu (Michael Dunlap)
Date: Thu, 9 May 1996 09:40:36 -0700
Subject: Re: Parts for Zeiss EM109

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X-Sender: szmdunla-at-peseta.ucdavis.edu
Message-Id: {v02130502adb7d05bc11c-at-[128.120.187.4]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"



} Am looking for replacement parts for the ion getter pump on the Zeiss EM109,
} in particular, the getter grids. The pump is a Leybold-Heraeus IZ80.

David

We just turned off our EM109 because of the ion getter pump. The grids can
be repaired with strips of titanium. For some reason we had a dozen or so
in the lab. I was able to make many repairs to the pump before the ceramic
insulators started to break down. Zeiss wanted a fortune for the
replacement parts. The instrument was not used very much so we are in the
process of selling it. A general announcement to the microscopy list will
be made in June.

Leybold USA does not sell the replacement parts, but Duniway Stockroom
(800-446-8811) was interested in repairing the Triode. However, they could
not guarantee that they could fix it.

Good luck

Mike

===========================================================
Michael Dunlap lab (916) 752-0284
Facility For Advanced Instrumentation fax (510) 422-2282
University of California mrdunlap-at-ucdavis.edu
Davis CA, 95616 http://128.120.187.6/
============================================================






From: taylor-at-iris1.sb.fsu.edu (Kenneth A. Taylor)
Date: Thu, 9 May 1996 13:55:18 -0600
Subject: Varian VE-10 vacuum evaporator

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Message-Id: {9605091753.AA01778-at-iris1.sb.fsu.edu}
X-Sender: taylor-at-iris1.sb.fsu.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hello,

Is there anyone in the EM community who has an operating manual or
schematics, anything at all, on a Varian VE-10 vacuum evaporator. I have
acquired one that seems to be in reasonably good condition and would like
to use it for some experiments. However, we have not found any
documentation on this particular model evaporator here. It is a VE-10,
model 961-1000, SN 1151. Varian have not been able to find anything on
this because the model was last made in the 1970's and they sold the model
to a company called Thermionics, based at the time in Hayward, CA. My hope
is that someone out there has a working model and would be willing to send
me a Xerox of the operating manual and any schematics so that we can
trouble shoot the one we have here.

Thanks in advance for any help you can give us.


{ {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } {

Kenneth A. Taylor, Ph.D. Phone: 904-644-3357
Institute of Molecular Biophysics Fax: 904-561-1406
Florida State University E-mail: taylor-at-sb.fsu.edu
Tallahassee, FL 32306-3015
Home pages: http://www.sb.fsu.edu/~taylor/
http://www.fsu.edu/~biology/faculty/kat.html

{ {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } {








From: Colin Veitch :      C.Veitch-at-geel.dwt.csiro.au
Date: Thu, 9 May 1996 20:54:13 -0500
Subject: Thanks regarding beam current measurement

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G'day

My thanks to all those who responded to my question regarding beam current
measurement on a JEOL 2010. It appears that Gatan didn't include the
"spacer" to pull the sample holder back that 5mm. I've contacted the agents
in Australia and they are looking into it.

Thanks again

Colin Veitch
#####################################################################
# #
# Colin.Veitch-at-geel.dwt.csiro.au #
# Instrumentation Scientist #
# CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 #
# P.O. Box 21 Fax. +61 (0) 52 275657 #
# BELMONT Vic 3216 #
# Australia #
# #
# "We see the Universe the way it is because if it were different, #
# we would not be here to observe it." #
# #
#####################################################################





From: Martin Newman-1 :      Martin_Newman-1-at-sbphrd.com
Date: 9 May 96 16:59:34 EDT
Subject: Position available - Microscopy & Flow Cytometry,UK

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Message-Id: {9605091859.AA5350-at-pho903.sbphrd.com}
To: Microscopy {Microscopy-at-Sparc5.Microscopy.Com}

Position available as advertised in New Scientist (Thursday 2nd May 1996)
VACANCY FOR A BIOLOGIST - ANALYTICAL SCIENCES, Harlow, Essex, UK.
You will join our microscopy and flow cytometry group which comprises a number
of talented scientists who provide the
creativity and expertise needed in a large and challenging R&D environment.
They have one of the longest-established and
best-equipped units of its kind in the industry and exploit state-of-the-art
facilities across a broad spectrum of research areas
and development activities, including neurosciences, vascular biology and
anti-infectives research. You should have a proven
interest in any major area of modern microscopy and/or flow cytometry,
preferably at doctoral/postdoctoral level, backed by
demonstrable skills. This includes any aspect of biological light microscopy
and electron microscopy, including
cryoTEM-electron crystallography of biomolecules and biological atomic force
microscopy. Ref: BAS/NS.

Please do not reply to the sender of this e-mail, but send your CV directly to:
The Human Resources Manager, SmithKline Beecham Pharmaceuticals, Old Powder
Mills,
Leigh, near Tonbridge, Kent TN11 9AN, UK. Closing date: 26 May 1996.






From: Charles.P.Daghlian-at-Dartmouth.EDU (Charles P. Daghlian)
Date: 09 May 96 12:50:50 EDT
Subject: EM rates

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Message-id: {14611868-at-prancer.Dartmouth.EDU}

Greetings to all,

I am trying to find out if other labs have bulk rates for use of their electron
microscopes.
You may recall that there was a series of messages regarding problems the
University of Hawaii had with federal audits. This request is related to that
thread.
As a part of Dartmouth's effort to comply with the various cost accounting
regulations (OMB, A-21, A-133, etc.), I need to try to get an idea of how other
labs are handling bulk time purchases from research labs in their institutions.
Specifically, do other labs permit bulk purchases of time, or is everyone
required to pay on an hourly (or some other) basis? Is you lab a departmental
or institutional facility? What level of support do you receive from the
university/college (% of operating budget)? Is there a rational basis for your
rate structure?

To get things started -- This is an institutional lab. For in-house users we
charge $40 per hour for microscope time, and at the moment, charge $3,000 per
year for up to two individuals per grant for unlimited access to the
microscopes. We are heavily subsidized by the various parts of the college (to
the extent of about 80% of our operating budget). We are working on making the
rates more rational without eliminating all activity.

I would be happy to provide a list of responses, if any come in.

Note that the regulations mentione above will effect all colleges and
universities that receive federal support, so many of you will probably want
similar information at some point in time.

Thanks in advance,

Chuck
*************
Charles P. Daghlian, Ph.D.
Director, Rippel E. M. Facility
Dartmouth College, HB 7605
Hanover, NH 03755
phone 603-646-1039




From: krogers-at-ecn.purdue.edu (Kirk Rogers)
Date: Thu, 9 May 1996 13:05:21 -0500
Subject: FW:Job Position Available

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} Date: Thu, 9 May 1996 06:08:23 -0500 (CDT)
} From: MASONW {william.mason-at-bbsrc.ac.uk}
} To: Multiple recipients of list {nih-image-at-Soils.Umn.EDU}
} Subject: Job Position Available
}
} PRODUCT MANAGER
} BIOMEDICAL RESEARCH IMAGING
}
} Fast growing company developing and selling computer-based products
} for biomedical and biotechnology research imaging, using fluorescent
} microscopy, seeks a dynamic, outgoing recent graduate or postgraduate
} in biological sciences or related discipline. You might have experience in
} imaging or photometric applications, or both, using optical probes for
} microscopic applications including ion imaging, FISH or other similar
} techniques on living or dead cells.
}
} You will act as our key product specialist and represent the company to both
} end users and distributors worldwide. Computer literacy important, and
} interest or experience in biomedical sales or marketing will be of
} assistance in this important role. This is a challenging opportunity based
} in the attractive university town of Cambridge, England, offering exciting
} growth prospects, possibility for worldwide travel and an attractive
} compensation package for the right individual.
}
} Apply with CV by post, fax or email to:
}
} Dr. William Henderson
} Managing Director
} Life Science Resources
} Church Street Barns
} Great Shelford
} CAMBRIDGE CB2 5EL
} United Kingdom
} Tel: 44-(0)1223-845836
} Fax: 44-(0)1223-840342
} Email: HENDERSON -at- LSR.CO.UK
}

-Kirk
_____________________________________________
Kirk Rogers krogers-at-materials.ecn.purdue.edu
OR kirk.a.rogers.1-at-purdue.edu
Purdue University, School of Materials Science and Engineering,
1289 MSEE building, W. Lafayette, IN 47907-1289
OFFICE: 317-494-8751 FAX: 317-494-1204
http://materials.ecn.purdue.edu/~krogers






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Thu, 9 May 1996 22:16:13, -0500
Subject: Asbestos standards

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-- [ From: Charles A. Garber., Ph.d * EMC.Ver #2.10P ] --

Renzo Garlasco wrote the following:
================
I use LM with polarized light and contrast liquids (potassium iodo
mercurate in water/glycerol) at 200 and 500 X. For Chrysotile I use
also nitrobenzene or mixtures of cinnammic aldehyde and benzyl alcohol.
I have a Chrysotile standard.

My problem is to manage other asbestos fibers, if present. How I can
see the other asbestos fibers: Crocidolite, Tremolite, Anthophyllite,
Amosite and Actinolite ? I don't have standards with those fibers. Some
of you can help me ?
======================================
For calibration and testing SPI has been offering the original UICC
(Union Internationale Centre le Cancer) reference specimens, in 0.1
gram vials, for chrysotile, anthophylite, amosite, and crocidolite. Use
as "knowns" for SAED, EDS, and XRD. Use as morphological standars for
TEM and SEM. This might not sould like a lot of material but for most
people it is a lifetime supply. Contact SPI below for pricing and
other information.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.com

####################################
WWW: http://www.2spi.com
####################################
======================================================





From: Ian MacLaren :      MACLARIZ-at-novell2.bham.ac.uk
Date: 10 May 1996 10:41:27
Subject: Re: TEM sample prep of SiC fibers?

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On Thu, 9 May 1996, XiaoGuang Ning wrote:

} Dear Microscopists:
}
} Could you please tell me how to well prepare for a TEM sample of SiC
} fibers in order to observe their cross-sections???
}
} Your help is appreciated very much.
}
} X.G.NING
}

Two possibilities at least:

1 - try microtomy. It seems rather unbelievable but it is maybe the only=20
way to have a rather exact idea of the fiber surface. Fiber surface is=20
important because properties of the composite depend on interface=20
fiber-matrix. You may read a letter I wrote a few years ago:

=ABMicrotomy : a convenient method for preparing Transmission Electron=20
Microscopy in ceramic science=BB, Jour. Mater. Sci. Lett., 9 (1990), 48.

(with a typographic mistake on top of page 49: the summit of the pyramid=20
is .2 mm, not 2mm.)
I can send a copy if needed. Please contact me off line.


2 - "Michael K. Cinibulk" {cinibumk-at-ml.wpafb.af.mil} posted a quite=20
interesting message a few months ago, about an improved ion mill method.=20
copy hereafter.

sincerely,

Yves MANIETTE

******BEGINNING OF MESSAGE*****


} Dear Microscopists:
}
} Could you please tell me how to well prepare for a TEM sample of SiC
} fibers in order to observe their cross-sections???
}
} Your help is appreciated very much.
}
} X.G.NING

I recently asked a similar question and received a whole bunch of replies
which are reproduced below.

Hope this helps

____________________________________________________________________________
My original message:

Dear all,
Has anyone out there prepared non-conducting ceramic fibres for TEM, either
across or along axis? If so, how did you do it?
We have some mullite fibres we would like to look at and although we have
some ideas about how to prepare specimens we would appreciate any ideas or
feedback on this topic.
____________________________________________________________________________
We have been routinely preparing ceramics fiber tows (Nextel, Nicalon, HPZ,
etc.) with and without ceramic coatings for TEM characterization for about
two years now. After much trial and error with a number of methods (some in
the literature) we have settled on a rather efficient method of producing
high-quality sections. We are just finishing a short communication on this
technique, which I could send to you when completed.

First, the tows are vacuum-impregnated with a high-temperature epoxy, such
as G-1. It is important that the fiber-volume fraction be high, which can
be achieved by either pulling many tows through the tip of a pipette, or
alternatively using shrink tubing. The epoxy is then allowed to cure.

The key to obtaining a good thin section with a large electron transparent
area is to minimize ion-milling time, which requires mechanically thinning
the sample to thicknesses less than 5 um, ideally less than 1 um. We use a
tripod polisher with 3M diamond lapping films to minimize differential
polishing and surface relief. The epoxy-impregnated tows have been thinned
both axially and transversely; either way wil produce good specimens.
Again, you want to thin the specimen as much as possible prior to ion
milling; if the specimen is too thick the epoxy will be milled away before
the fibers and any coating becomes electron transparent, with the fibers
usually falling out. We also need the epoxy to bound the coating for
coating thickness determination.

The specimen is then mounted on a Cu washer, and maybe a grid, and ion
milled at low angles for 30-60 min.

For more details please contact me off line.

Dr. Michael Cinibulk cinibumk-at-ml.wpafb.af.mil
Wright Laboratory/MLLM
Wright-Patterson Air Force Base, Ohio
____________________________________________________________________________
Do you have a tripod polisher in your prep lab.

We have a way of prepping fibers using a tripod that worked nicely
for cross and longitudinal sections of SiC fibers.

Ron Anderson
____________________________________________________________________________
I assume that you are aware of the difficulties in doing this. I know of
two methods which work very well and are based on other, relatively
well-known techniques for producing cross-sections.

A) embed in resin and ultramicrotome cross-sections; this is only
convenient if you have someone who does ultramicrotomy already, in which
case it is probably the simplest.

B) sandwich with epoxy between two silicon wafers and then cross-section
'normally,' just like a VLSI device; by visually monitoring optical
transmission through the silicon, you can thin the composite structure well
below 10 micrometers before ion-milling (which in turn helps mitigate
differential milling effects). This works for almost everything. The same
basic technique could be used to thin the fiber along the axis.

Please let me know if something easier comes along, since I have to be
doing this as well...

Daniel L. Callahan
Assistant Professor of Materials Science
Rice University
http://www.owlnet.rice.edu:80/~dlc/
____________________________________________________________________________
I had pretty good luck preparing ceramic fibers (mullite,
SiC, Al2O3/ZrO, and others), of 10 to 30 micron diameter,
for TEM analysis. The method is very simple, and consists
of first making a composite out of the fibers and an epoxy,
then thining down as with a bulk sample (polishing,
dimpling, ion milling). Things to keep in mind:

1. If the fibers are short and tangled as a small cotton
ball, saturate the epoxy with the fibers mixing very well.
The fibers will brake but that doesn't matter since is
unlikely that you will be interested in crystalline defects.
Squeeze the composite between two teflon or other
non-sticking sheets using glass slides for backings.


If the fibers are long (more than 3mm) you can lay down
a thin layer of epoxy on a teflon sheet, and the fibers on
top, one at a time or in bundles, in a single direction to
cover a strip 3mm wide x length of the fibers. Then squeeze
between two glass slides and another teflon sheet covered
with epoxy. Cure the epoxy as per manufacturer instructions.
In both cases you will be able to form a very thin composite
that requires a little grinding and polishing on both
sides before punching out 3mm discs, which can be further
polish to 100 micron. Dimple as usual (with diamond paste)

2. Epoxy: try with whatever you have available. Some
people prefer conductive epoxies. Initially, I used several
conductive and non conductive products from TRA-CON
(Medford, MA, 508-391-5550), but for some time now I have
settled on G1 epoxy from GATAN (412-776-5260).

3. Ion Milling: the mullite I worked with milled very fast
probably because it was very porous. Other ceramics mill
very slowly compared to the epoxy. This is a problem that
can be alleviated using a LN2 stage in the ion mill, to slow
down the milling rate of the epoxy, and having as much
fiber content as possible in the mixture.

4. Even if you use a conductive epoxy you may have to
carbon coat the thinned samples.

Good Luck!

Augusto Morrone
Univ. of Florida
Materials Science and Engineering
P.O.Box 116400
(352) 392-6985
amorr-at-mse.ufl.edu
____________________________________________________________________________
been using the Tripod polisher and a unique technique for tightly binding
fibers together. They have been working with SiC fibers, but I think that it
would work for you. Their Email addresses are
CINIBUMK-at-ml.wpafb.af.mil and SCHELTFJ-at-ml.wpafb.af.mil

- -Scott Walck
____________________________________________________________________________
I think that someone here used a Gatan PIMS to do this several years ago
with some success. The length of the fiber went across the 3mm washer.
John Hunt
____________________________________________________________________________
Over the years I've prepared various fibre materials in various ways. These
all should give results; the quality varies, but then so does the effort
required.

1) If the fibres are thin to begin with, pop them onto a support film
covered
grid, carbon coat, and voila!

2) If you have experience with ultramicrotomy, embed and section small
areas.

You may require a coupling agent to promote resin adhesion, and the result
of sectioning will be a mass of shattered fragments (unless the fibres are
amorphous), but then you might also get a huge amount of viewable area free
of chemical contamination.

3) Mix the fibres into a particularly hard resin such as Petropoxy which you
can then prepare (cut, grind, dimple, ion mill or tripod) as a monolithic
lump. I'm not aware of any resin hard enough to thin at the same rate as a
ceramic, but I've prepared SiC fibres this way with success.

4) Create a composite by electroless deposition of metal (usually Ni) which
you can then prepare in the ion mill. This one gives by far the best result
in my experience, but is also the trickiest and most potentially time
consumming. A short electroless plate to make the fibres conductive ,
followed by electrolytic deposition in standard plating baths is a good
option if you have access to such. The method I've used was adapted from
metal powder prep. techniques.

Let me know if you'd like further information on any of this.

###############################################################
# #
# Don Steele STEELE-at-KRDC.INT.ALCAN.CA #
# ALCAN INTERNATIONAL #
# Kingston Research and Development Center #
# P. O. Box 8400 #
# Kingston, Ontario Canada K7L 5L9 #
# #
###############################################################
____________________________________________________________________________
The answer is simple (at least in principle!) - embed them in a hard
epoxy resin or acrylic, then section them in an ultramicrotome,
collect on fine-meshed grids, pop in the TEM, and ---presto, nice
uniformly thin fiber cross-sections held together by the resin.
There will undoubtedly be some tearing of the section and/or
individual cross-sections. The fibers will be randomly dispersed so
that you'll get many orientations (inlcuding some near-longitudinal
slices, if they're useful). Be careful of beam effects - most
embedding materials are somewhat to terribly beam sensitive.

Sound too easy to be true? You're right, ultramicrotomy is somewhat
of an art, but can accomplish wonders in materials science. A good
reference is an overview by yours truly:

T.F. Malis and D. Steele, Specimen Preparation for Transmission Electron
Microscopy of Materials, MRS vol 199, Materials Research Society (1990) p.3.

If you don't have any MRS Proceedings there, I have a few copies left and
can send one, but only if you're seriously interested, as they're in short
supply. The people at UMIST in the Corrosion Center are experts in
this area as well. I tend to act as a information bank for this technique,
so let me know how you fare - I'm always looking for new references to
quote.

Tom Malis
Group Leader - Materials Characterization
Materials Technology Laboratory
Natural Resources Canada (Govt. of Canada)
Ottawa, Ontario
ph.: 613-992-2310
FAX: 613-992-2310
e-mail: tom.malis-at-cc2smtp.emr.ca
____________________________________________________________________________
This may or may not be applicable, but with the high-voltage EM
(lower interaction cross-section) and very low beam currents, we have been
able to image non- or poorly-conducting specimens without charging or heat-
ing problems. If this holds true for your instrument (e.g. you have an
IVEM), the advantage is that there may be no preparation required; the
disadvantage is that with these low doses you will either need a very sen-
sitive recording medium--LoDose or other x-ray film, image plates or in-
tensified or slow-scan CCD--or long exposures. The large grain or speci-
men drift could make the images useless. Good luck.
Yours,
Bill Tivol
____________________________________________________________________________
I am a little late in responding to your inquiry on ceramic fibers; My
apologies. Since some of the other responses mentioned using the wedge
technique for preparing thin TEM sections, I wanted to mention that BUEHLER
KKB, with whom I am affiliated, is a supplier of a wedge polishing tool such
as those mentioned. I previously was Applications Engineer for South Bay
Technology who offered the first commercially available tripod based
polisher. However, I have since come to work for BUEHLER, LTD in the US,
and we have made some changes to the IBM design in order to enhance the ease
of producing wedge samples. We also offer a complete line of diamond
lapping films for this polishing system.

If you would be interested in more information regarding BUEHLER's
MICROPRECISE(TM) Tripoint Polisher, I would be happy to have literature sent
to you, and/or have a salesperson contact you. If you would like more
information, please feel free to contact me directly by phone, fax or
e-mail.

Best regards,
Scott D. Holt
BUEHLER, LTD.
41 Waukegan Rd.
Lake Bluff, IL 60044 USA
Phone: (847)295-4546
Fax: (847)295-7942
102467.2752-at-compuserve.com
____________________________________________________________________________


_________________________________________________________________
Ian MacLaren, Telephone: 0121 414 3447
IRC in Materials, FAX: 0121 414 3441
The University of Birmingham, email: I.MacLaren-at-bham.ac.uk
Birmingham B15 2TT, England.
_________________________________________________________________




From: Trevor Sewell :      SEWELL-at-uctvms.uct.ac.za
Date: Fri, 10 May 1996 16:15:36 +0200
Subject: Re: Parts for Zeiss EM109

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} Am looking for replacement parts for the ion getter pump on the Zeiss EM109,
} in particular, the getter grids. The pump is a Leybold-Heraeus IZ80.

Last year we purchased a vacuum pump upgrade kit from LEO Oberkochen
to replace the IZ80 with a TPH 240 turbopump on the EM 109.

It worked fine and has made a great difference to the usability of
the microscope.

Trevor Sewell
EM Unit
University of Cape Town





From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 10 May 1996 13:48:57 -0400 (EDT)
Subject: Re: EM rates

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Dear Charles,

} I am trying to find out if other labs have bulk rates for use of their
} electron microscopes.

We have contracted for blocks of time, but we are a NY state fa-
cility, so commercial users are not encouraged to buy time on the HVEM.

} I need to try to get an idea of how other labs are handling bulk time
} purchases from research labs in their institutions.

We do it on a case-by-case basis--sorry I can't be more helpful.

} Is you lab a departmental or institutional facility?

Yes, but we are also funded my NIH as a Biotechnological Resource.

} What level of support do you receive from the
} university/college (% of operating budget)?

50% of our support comes from NY state; 50% from NIH.

} Is there a rational basis for your rate structure?

Yes. Since NY is not allowed either to sell services at a profit
or to give services away at less than cost, someone must calculate the
total cost of operating the HVEM (including bldg maintenance, etc.), and
we charge that cost--~$207/hr. A contract for $5000 (which happened within
the last decade) will buy ~25 hr.
}
} For in-house users we
} charge $40 per hour for microscope time,

No charge for in-house users or not-for-profit (incl academic) out-
side users.

} and at the moment, charge $3,000 per
} year for up to two individuals per grant for unlimited access to the
} microscopes.

No analogous arrangements are allowed here (to the best of my know-
ledge).

} We are heavily subsidized by the various parts of the college (to
} the extent of about 80% of our operating budget). We are working on making
} the rates more rational without eliminating all activity.
}
See above for our solutions.
Yours,
Bill Tivol




From: Hand-at-nso1.uchc.edu (Hand,Arthur)
Date: Fri, 10 May 1996 14:51:33 -0400
Subject: RE: Bubbly Methacrylate

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Dear Nigel:
We have had a similar problem when polymerizing Lowicryl K4M in a UV
chamber, probably due to too much heat generated during the polymerization.
You can try reducing the intensity of the UV light, or use indirect light
rather than direct light (if you have a foil lined chamber, put a foil
covered baffle between the light and the specimen). A smaller volume of
resin might also help (e.g. a smaller gelatin capsule). What works well for
us is to use disposable polyethylene flat molds (available from E. Fullam, I
think), overfilled with resin and covered with Saran wrap to exclude air.

Arthur R. Hand
Central EM Facility
UConn Health Center
Farmington, CT 06030 USA





From: Dave King (607)757-1248 T37/257-3 Ext DEKING-at-VNET.IBM.COM
Date: 10 May 1996 14:51:47 EDT
Subject: TFSS Algorithm?

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Message-Id: {199605101909.OAA02439-at-Sparc5.Microscopy.Com}

To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}


We do a calculation with WDS data, which calculates the
equivalent thickness and micro-grams of a material sitting on a
surface. It's written in APL, an IBM programming language which
is not used much any more.

We would like to understand the calculation, and possibly use a
spread sheet to do the calculations and store the results for
future reference. I bet it uses a bunch of ZAF type corrections,
and may therefore be very involved, primarily due to the vast
number of correction factors needed.

There is a T/N program called TFSS, which may do essentially the
same calculation. We could use it on our 5500 system, and I
should see if the results are similar between the 2. Still, I'd
have the same problem of blindly feeding numbers in, and
accepting the results.

Does anyone know of a report that explains how to do this
calculation? Thanks,

. {
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: Elinor Solit :      cambrex-at-world.std.com
Date: Fri, 10 May 1996 16:44:46 -0400 (EDT)
Subject: Re: Microtome

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Hi Eric,

If you send me your address, we'll mail you the new catalog "The
Microscope Book". I think it will be helpful to you, especially if used
in conjunction with its Web page: http://www.shore.net/~catalogs.

Call us if you have any questions. And have a great time with your new
"dream microscope".

Regards,

Elinor Solit
Director of Publications
The Cambrex Group


On Thu, 9 May 1996, Eric Chapman wrote:

} I have just obtained my dream microscope and and trying to get set up in my
} home for a small lab. I need to purchase a microtome. Also, I would like a
} line on glassware and slides. I have the VWR Scientific catalog but was
} wondering if there are other vendors I might write to for catalogs.
}
} If any of you have phone numbers or addresses I would appreciate it. Also,
} if anyone has a used microtome for sale I would love to hear from you.
}
} ---------------------------------------------------------------
} Eric Chapman - Eagle River Alaska _/_/_/ _/_/_/ _/_/
} Hemodialysis Patient _/ _/ _/ _/ _/
} _/ _/_/ _/
} Sic Gorgiamus Alles _/ _/ _/ _/ _/
} Subjectamos Nunc _/_/ _/_/_/ _/_/
} ---------------------------------------------------------------
}
}




From: phhrich-at-asterix.helix.net (PHH Environmental)
Date: Fri, 10 May 1996 14:27:29 -0700
Subject: Re: LM - polarized - Asbestos

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Message-Id: {199605102111.OAA26205-at-helix.net}
X-Sender: phhrich-at-helix.net
X-Mailer: Windows Eudora Version 1.4.4
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

At 21:10 09/05/96 +0200, Garlasco wrote:
} Dear All,
}
} I use LM with polarized light and contrast liquids (potassium iodo mercurate
} in water/glycerol) at 200 and 500 X.
} For Chrysotile I use also nitrobenzene or mixtures of cinnammic aldehyde and
} benzyl alcohol. I have a Chrysotile standard.
} My problem is to manage other asbestos fibers, if present.
} How I can see the other asbestos fibers: Crocidolite, Tremolite,
} Anthophyllite, Amosite and Actinolite ? I don't have standards with those
} fibers.
} Some of you can help me ?
}
} Thank you !!
} } Renzo Garlasco
} } garlasco-at-newsoft.it
_____________________________________________________________________________

Dear Renzo,

The current standard, and probably the most widely used method for the
identification of asbestos is polarized light microscopy (PLM). The
condition of having crossed polars on your LM will allow you to see many of
the optical characteristics that you can use to identify the six main forms
of asbestos. These characteristics can include: pleochroism, anisotropy,
birefringence, extinction angle, and sign of elongation.

To conclusively identify the asbestiform minerals, you should use a special
objective with a 'stop' ( a black disc) placed in the objective back focal
plane. Such an objective is called a 'dispersion staining objective'. The
addition of this 'stop' will allow you to see 'dispersion staining colours'.
These colours represent certain wavelengths of light that correspond to the
specific refractive indexes of the fibre or particulate in question. This
optical staining technique is referred to as 'dispersion staining'.

The refractive index values obtained from the dispersion staining colours
are used in conjunction with the other optical characteristics already
obtained to identify Chrysotile, Crocidolite, Tremolite, Anthophyllite,
Amosite and Actinolite, as well as other fibres and particulate.

Refractive Index liquids such as those made by Cargille Laboratories are
used as the mounting medium for PLM identification of asbestos fibres.
Based on a preliminary stereoscopic examination with a standard stereoscope
in a fume hood, different Refractive Index liquids are used depending on
which asbestos type is suspected.

Certified bulk standards of the asbestiform minerals can be obtained from
the National Institute of Standards and Technology.

For more information please e-mail me at: phhrich-at-phhenvironmental.com

Dennis Nordlund
PHH Environmental Limited

URL: http://www.phhenvironmental.com
_______________________________________________________________________________





From: Daniel Schwartz :      dschwartz-at-mdc.com
Date: Fri, 10 May 1996 17:46:02 -0500
Subject: Ultramicrotoming SiC fibers

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Message-Id: {s1938221.081-at-mdc.com}
X-Mailer: Novell GroupWise 4.1

Just a quick note of caution to add to the thread about
preparing SiC fibers for TEM: SiC is hard enough to
damage a diamond knife. My experience with
microtoming SiC is all negative: it nicked my knife, and
the SiC was basically shattered. I would stick to tried
and true embedding, dimpling, and ion milling
techniques (or is that tired and true?), or tripod
polishing.

Dan Schwartz, McDonnell Douglas Aerospace





From: azriel gorski :      azrielg-at-cc.huji.ac.il
Date: Sat, 11 May 1996 10:57:35 +0300 (GMT+0300)
Subject: Re: Microtome

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There are MANY companies where you can obtain catalogs, but some are more
convient than others. Some sales reps are more "helpful" than others. What
I would suggest, if you can, drop into your nearest college or University
and visit in the Biology or like department that uses microscopes. Go to
the Department Office, and ask if you could talk to one of the faculty.
Most college/University departments are very open to "strange requests."

Explain to her/him that you are an newly starting "amateur" microscopist
and you are looking for sources of supply for equipment and miscellaneous
supplies. You may be refered to another faculty member who is more
their "resident microscopist." I would be very supprized if you did
not meet more than one faculty memeber. They have the catalogs, and *know*
who is local and gives good service.

You might also find a friendly ear, and see many things you had not
thought about.

Enjoy your microscope.

Shalom from Jerusalem,
Azriel Gorski
Head, Optical Microscopy Laboratory
Israel National Police


On Thu, 9 May 1996, Eric Chapman wrote:

} I have just obtained my dream microscope and and trying to get set up in my
} home for a small lab. I need to purchase a microtome. Also, I would like a
} line on glassware and slides. I have the VWR Scientific catalog but was
} wondering if there are other vendors I might write to for catalogs.
}
} If any of you have phone numbers or addresses I would appreciate it. Also,
} if anyone has a used microtome for sale I would love to hear from you.
}
} ---------------------------------------------------------------
} Eric Chapman - Eagle River Alaska _/_/_/ _/_/_/ _/_/
} Hemodialysis Patient _/ _/ _/ _/ _/
} _/ _/_/ _/
} Sic Gorgiamus Alles _/ _/ _/ _/ _/
} Subjectamos Nunc _/_/ _/_/_/ _/_/
} ---------------------------------------------------------------
}
}





From: OptoMech-at-aol.com
Date: Sun, 12 May 1996 21:02:29 -0400
Subject: general

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unsubscribe




From: OptoMech-at-aol.com
Date: Sun, 12 May 1996 21:02:30 -0400
Subject: Re: Bulbs

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We carry a wide variety of bulbs for microscopy. Please call, we would be
glad to help you.

Eric Schrader
OMC
2102 Riding Crop Way
Baltimore, MD 21244
410-944-1721
410-265-5873 fax





From: Robert McDonald :      robert-at-geology.gla.ac.uk
Date: Mon, 13 May 1996 14:36:16 +0100
Subject: Polishing Gold

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Message-Id: {21658.199605131336-at-starav.geology.gla.ac.uk}


Hi All in Microland:

Does anyone have any tips for polishing gold to use as a standard?

I have available only very basic polishing equipment which generally
serves our needs for polished rock sections very well using diamond
paste of varying grades.

My problem seems to be that the gold is so soft that when I jump from
one micron diamond to 0.25 I only make things worse.

Any advice would be most welcome.

Robert McDonald
Geology & Applied Geology Dept
Glasgow University
Glasgow
Scotland

UK




From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 13 May 1996 11:18:22 -0400 (EDT)
Subject: Re: longevity of direct-drive mechanical pumps

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}
} Hi, The old Edwards belt driven pumps are heavily constructed and run at a
} lower rotational speed than the new direct-drive units. This is sort of
} typical of the new construction philosophies. I repair older Philips
} Electron Microscopes so I have quite a bit of first-hand experience with
} the older pumps. They are rugged but do not always maintain an optimum
} pumping speed. Most people don't notice a decrease in pumping speed, so
} they happily let their old pumps chug along for decades. I have replaced
} about six belt-driven units with the newer, direct drive pumps but I
} certainly don't expect them to last as long as the old ones did. The higher
} speed, lower volume of oil and thinner castings all contribute to the
} longevity problems.
}
Dear Alex,
We have TC gauges on our belt-drive pumps, and the ultimate vacuums
for backing the turbopumps (~5 microns) and roughing the column (~50 microns)
have not changed for 15 years. We haven't measured the pumping speed direct-
ly, but the pumpdown times for the column and accelerator do not seem to have
changed.
Yours,
Bill Tivol




From: imgp-at-mbimp1.mbl.tno.nl (Kees van der Wulp)
Date: Mon, 13 May 1996 17:53:29 +0100 (MDT)
Subject: Hough transform

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Dear colleagues,

To find straight lines and circular objects one can apply the linear
and circular Hough Transform.
This transform is not present in my 'C' based IP package (SCIL-Image).

Question :

Does anyone of you know good comprehensive (extensive) literature
refs on this subject from which I can write the Houigh Transform programs
or even better ...... is there someone out there who has the C routines
to calculate the lin. & circ. H.T. and is prepaired to grant them to me ?

I am grateful for any help !

Kees.

BTW. The Image Processing Handbook does not cover the subject in depth
in order to write my own programs.

--
Kees van der Wulp

TNO - Prins Maurits Laboratory INTERNET : vanderwulp-at-voeding.tno.nl
Division 1 New VOICE : +31 15 2843101
Department : ATES New FAX : +31 15 2843963
PO-Box 45 General TNO Info : http://www.tno.nl
2280 AA RIJSWIJK (NL)
THE NETHERLANDS




From: Eric Steel :      steel-at-enh.nist.gov
Date: Mon, 13 May 1996 09:57:23 -0400 (EDT)
Subject: asbestos standards for polarized light microscopy

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In reply to Renzo Garlasco's request for info on asbestos standards:

There are two National Institute of Standards and Technology Standard
Reference Materials (SRM) specifically designed for the polarized light
microscopic identification of asbestos, unluckily they are not free.

Here is a brief description of the materials, so that you can see if you
might be interested:

Standard Reference Material 1866 "Common Commerical Asbestos" consists of
chrysotile, Amosite, crocidolite, and a glass fiber blank and Standard
Reference Materials 1867 "Uncommon Commercial Asbestos" consists of
actinolite, tremolite, and anthophyllite. The optical properties and the
asbestiform nature of the asbestos materials are certified. The certified
optical properties include morphology, pleochroism, birefringence,
extinction, sign of elongation, and the refractive indices of the material
as a function of orientation and wavelength. Crocidolite is an exception,
because of its strong absortion, refractive indices are reported but not
certified. You get several grams of each standard.

You can contact me for further technical information on the standards or for
information about obtaining the standard you may contact:

Standard Reference Materials Program
National Institute of Standards and Technology
Gaithersburg, MD 20899-0001
USA

Phone: 301-975-6776
FAX: 301-948-3730
e-mail: SRMINFO-at-enh.nist.gov


Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov
Microanalysis Research Group Office: 301-975-3902
N.I.S.T. FAX: 301-216-1134
Bldg. 222/Rm A113
Gaithersburg MD 20899





From: Joe D Geller :      geller-at-world.std.com
Date: Mon, 13 May 1996 15:00:24 -0400 (EDT)
Subject: Polishing gold

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We also use diamond for the polishing. The cloth used we have found to be
critical. You must use a soft one like Buehler's "Microcloth" with very
light pressure. Nylon produces unacceptable results.

Joe Geller
Geller MicroAnalytical Laboratory
426e Boston St.
Topsfield, MA 01983
jg-at-gellermicro.com

Manufacturer's of gold standards, and other materials, for x-ray
microanalysis.




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Mon, 13 May 1996 10:52:02, -0500
Subject: Ceramic fibers prep

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Message-Id: {199605131452.KAA05972-at-mime3.prodigy.com}
X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc06

-- [ From: Charles A. Garber., Ph.d * EMC.Ver #2.10P ] --

In resonse to
=======
} Could you please tell me how to well prepare for a TEM sample of SiC
fibers in order to observe their cross-sections???
==========
There was quite a long list of extremely valuable information which we
found very useful. Thank you!

However there was no discussion on the technical merits of a "tri-pod"
polishing/ion milling vs. diamond knife thin sectioning approach. I
came to realize early on that nothing in the world of EM is without
artifacts, and the challenge more often than not is to make judgements
as to what is real and what is artifact. So I was wondering how those
with the experience, and those able to really compare the two methods
from first hand experience would comment on the matter of artifacts and
to what degree one approach is more artifact free than the other.

It has been our own experience, though I suspect very limited in
comparison to others who made postings, that the ion milling induced
artifcats are isotropic and therefore not so easy to spot where as the
ultramicrotomy induced artifacts are anisotropic in nature and to do an
"artifact test" one need only to rotate the sample in the microtome and
see whether the suspected artifact feature does or does not rotate in
the final image. So even though ultramicrotomy might in fact not have
fewer artifacts, those that are there are more readily recognizable as
to what they are.

Also, in response to the following by Dan Schwartz:
===================
Just a quick note of caution to add to the thread about preparing SiC
fibers for TEM: SiC is hard enough to damage a diamond knife. My
experience with microtoming SiC is all negative: it nicked my knife,
and the SiC was basically shattered. I would stick to tried and true
embedding, dimpling, and ion milling techniques (or is that tired and
true?), or tripod polishing.
===============================

Well it is a fact that cutting "hard" materials like SiC fibers will
more quickly wear down a diamond knife in the same way as quick starts
and stops will more quickly wear out automobile tires. However, in
some instances, diamond knives may in fact be a viable alternative, if
not in some cases, a more attractive alternative. True there is cost
but the real issue is the cost per sample possible per knife rather
than the cost of the knife itself. "Perfection of the art" enables one
to obtain acceptable sections faster using fewer passes of the sample
over the knife (e.g. less knife wear), and of course using "materials"
instead of "life" science diamond knives reduces the per sample cost
still further, and by more than a trivial amount. In fact in our own
laboratory we have got it down to the point whereby such a "hard"
sample adds typically to the client cost an incremental amount not
exceeding $150 per sample assuming there are multiple samples. But for
the uninitiated, that cost could be far greater. We know of some users
who have literally "broken" the knife the first time out.

Disclosure: SPI Supplies offers "Materials Science Diamond Knives"
(see on-line electronic catalog) and surely would like to see more
diamond knives being used up in the conduct of this kind of work! Also,
our analytical services laboratories will thin section "hard" samples
for clients as a service.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.com

####################################
WWW: http://www.2spi.com
####################################
======================================================




From: durmer-at-mail.med.upenn.edu (Jeff Durmer)
Date: Mon, 13 May 1996 17:16:30 -0400
Subject: unsubscribe

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unsubscribe me please.




Jeffrey Scott Durmer
*** "Esclavo de Rosenquist" ***
#########################################
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Univ. of Pennsylvania/Philadelphia,PA 19104
(215)898-7579-wk { { {-} } } (610)525-1393-hm
***durmer-at-mail.med.upenn.edu***
#########################################







From: Joseph Michael :      JRMICHA-at-sandia.gov
Date: 13 May 1996 15:51:51 -0700
Subject: Re:Hough Transform

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Dear Colleagues,

There is an excellent reference on the Hough Transform that covers both the
extraction of linear features and circular features. The title is

"Shape Detection in Computer Vision Using the Hough Transform"

by V. F. Leavers
Springer-Verlag
New York

ISBN 0-387-19723-0


Joe Michael




From: DON_STEELE-at-CCKRDC.CA.ALCAN.CA
Date: Tue, 14 May 1996 08:48 -0500 (EST)
Subject: Re: Ceramic fibers prep

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To add to what Chuck Garber has said re: the identification of
artifacts in microtomed vs. ion milled samples:

--------------------------------------------------------------------
"that the ion milling induced artifcats are isotropic and therefore not so easy
to spot where as the ultramicrotomy induced artifacts are anisotropic in nature
and to do an "artifact test" one need only to rotate the sample in the microtome
and see whether the suspected artifact feature does or does not rotate in the
final image. "
----------------------------------------------------------------------------

the fracture behaviour of crystalline materials during microtomy can sometimes
be used for a quickly identification of phase distribution in a mixed sample.
I've used this while studying activated alumina pellets, graphite anodes, and
while I've never confirmed it, I've become sufficiently familiar with the
fracture artifacts of intermetallic constituents in Al alloys to usually have a
good idea what they are by their morphology in the section.

As for which method I choose when preparing a sample (fibre or otherwise), it
depends on what I'm looking for, how much time I have, and where the results are
going to end up. Regardless of how clearly and quickly my microtomed "fragment"
may have shown the thickness of a surface layer, if an image is required for
publication, I'm likely to use an ion milled sample. If I'm concerned with
quick results where EDXS is required, then microtomy will likely be my method of
choice.


Don Steele




From: Ray Gilbert :      rtg2-at-leicester.ac.uk
Date: Tue, 14 May 1996 09:00:05 +0100 (BST)
Subject: spinning solutions for immunogold labelling

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Does anyone have an idea how long and how fast to spin IgG gold
solutions and BSA to help reduce background on en-grid imunogold
labelling.

I am specifically looking at p450 enzyme in lung tissue but am having
a bit of difficulty with high backgroud in my controls.

Thanks alot

Ray Gilbert
Hodgkin Building
University of Leicester
PO box 138
Lancaster Rd
Leicester
LE1 9HN
fax 044 (0) 116 252 5616
ph 044 (0)116 252 3042
e-mail rtg2-at-le.ac.uk




From: roy-at-bayou.uh.edu (Roy Christoffersen)
Date: Tue, 14 May 1996 08:53:53 -0500
Subject: High-quality overheads from EM micrographs

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Does anyone out there know how to make high-quality overheads from standard
B&W photographs (e.g., TEM images including HRTEM images)? I have always
made slides for my talks up until now. Just using the standard xerox
transparencies does not give very good results, but I have seen talks where
people use transparencies and they are remarkably good. Is there some trick
or method I am missing out on?

Roy Christoffersen
Texas Center for Superconductivity
3201 Cullen
Houston, TX 77204-5932
roy-at-bayou.uh.edu
(713) 743-8273
FAX: (713) 743-2787






From: Robert McDonald :      robert-at-geology.gla.ac.uk
Date: Tue, 14 May 1996 09:35:18 +0100
Subject: Re: Polishing Gold

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Message-Id: {3043.199605140835-at-moruisg.geology.gla.ac.uk}


Thanks to all those who mailed me a load of info on the above
subject.

I think I replied personally to all except Madeline at Rutgers Uni.
- I lost the header with your address - so thanks again everyone.

Robert McDonald

Warm and Sunny Scotland at last

robert-at-geology.gla.ac.uk




From: Henrik Kaker :      Henrik.Kaker-at-guest.arnes.si
Date: Tue, 14 May 1996 19:40:11 +0200
Subject: Metallographic Standard

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Hi All,

I'm looking for metallographic standard NP-2080, april 1975 from Crysler
Company. Thank you.

Henrik Kaker
SEM-EDS Laboratory
Slovenia
http://www2.arnes.si/guest/sgszmera1/index.html




From: Ray Gilbert :      rtg2-at-leicester.ac.uk
Date: Tue, 14 May 1996 11:07:36 +0100 (BST)
Subject: Spinning solutions for immunogold

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have an idea how long and how fast to spin IgG gold
solutions and BSA to help reduce background on en-grid imunogold
labelling.

I am specifically looking at p450 enzyme in lung tissue but am having
a bit of difficulty with high backgroud in my controls.

Thanks alot

Ray Gilbert
University of Leicester
e-mail rtg2-at-le.ac.uk




From: DVCCO-at-aol.com
Date: Tue, 14 May 1996 08:05:29 -0400
Subject: (Web Site)CCD Cameras/Digitizers

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* Monochrome 10 bit / 1024 gray level real time cameras for high sensitivity
low noise applications (digital) and (analog) outputs.

*PCI bus frame grabber boards plug and play with custom cables for digital 10
or 8 bit imaging and 10 bit analog inputs ! Also Mac PCI, Mac NuBus, Sun
S-bus boards.

Real Time 10 & 8 Bit Digital RS-422/RS-170 Analog CCD Monochrome Cameras

A San Diego, California based manufacturer of monochrome digital RS-422
(10 or 8 bits) and RS-170 (analog 10 bit video cameras) with S/N of } 62dB
in real time 30 frames/ second, with a defect free sensor and 100% fill
factor, has a new and informative web site!
--------------------------------------------------------------------
(((((((( The URL is: http://www.edt.com/dvc/dvc.html ))))))))))
--------------------------------------------------------------------
The company can in addition provide complete (plug and play) frame
grabbers for digital or (analog 10 bit / 1024 gray level PCI bus), Mac
Nu-bus or, Sun S-bus. SGI digital output option available, as seen on
new Hot Mix 11 html SGI CD, along with custom cables, Mac and Sun imaging
software and tunable electronic filters for mono 400-1100nm range and (RGB
sequential version.)
Feel free to e-mail requests directly, stating bus of interest, digital or
analog video interest, and application. Signal to noise / pixel gray level
depth is just as, or more important than just spacial resolution! Is your
camera offering only 7 bits/64 gray levels, or 50dB S/N at best, and a New
England snow storm when the gain is turned up? Are you facing the expensive,
only 8 bit, ticking 7.5 fps slow expensive digital cameras along with no
RS-170 analog output.
Full details on how to choose a CCD camera and various frame grabbers
listed by bus on the above web site.
Feel free to email your application, spectrial nm area of interest etc.
Thank you.

Richard Klotsche
DVC Company
dvcco-at-aol.com
619-444-8300
619-444-8321-fax





From: Paul Webster :      paul.webster-at-yale.edu
Date: 14 May 1996 13:46:54 -0400
Subject: Mab labeling

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Message-Id: {n1380040563.8017-at-QuickMail.Yale.edu}

Thanks for all the references, they will help in the quest. However, the quest
has not been completed.

What happened to the people who replied when I said that double labeling using
two mouse monoclonals had probably not been done before. I received some
interesting, and sharp, replies with some good examples of how two monoclonals
had been used to label the same tissue slices.

Best regards,

Paul Webster
Center for Cell Imaging
Yale University School of Medicine
http://info.med.yale.edu/cellimg





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Tue, 14 May 1996 13:34:01 -0600
Subject: LM: Stereoscope by Wild-Heerburg

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I am trying to locate information on a stereomicroscope, Model M650,
manufactured by Wild-Heerburg, serial no. 10682. Can anyone provide the
phone number of a dealer so I can inquire about parts? Many thanks.


#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: durmer-at-mail.med.upenn.edu (Jeff Durmer)
Date: Tue, 14 May 1996 15:45:35 -0400
Subject: rub me out!

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please unsubscribe me : durmer-at-mail.med.upenn.edu
thanks




Jeffrey Scott Durmer
*** "Esclavo de Rosenquist" ***
#########################################
Dept. of Neuroscience/121 Johnson Pavillion
Univ. of Pennsylvania/Philadelphia,PA 19104
(215)898-7579-wk { { {-} } } (610)525-1393-hm
***durmer-at-mail.med.upenn.edu***
#########################################







From: Robert McDonald
Date: 13 May 1996 14:36
Subject: Polishing Gold

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Robert McDonald {robert-at-geology.gla.ac.uk}


Hi All in Microland:

Does anyone have any tips for polishing gold to use as a standard?

I have available only very basic polishing equipment which generally
serves our needs for polished rock sections very well using diamond
paste of varying grades.

My problem seems to be that the gold is so soft that when I jump from
one micron diamond to 0.25 I only make things worse.

Any advice would be most welcome.

Robert McDonald
Geology & Applied Geology Dept
Glasgow University
Glasgow
Scotland

UK




From: shaf :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 14 May 1996 08:34:45 -0700
Subject: JEOL 840|6300 users: addendum

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Message-Id: {2.2.32.19960514153445.006b3a84-at-darkwing.uoregon.edu}
X-Sender: mshaf-at-darkwing.uoregon.edu
X-Mailer: Windows Eudora Pro Version 2.2 (32)
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Regarding my previous query: replacing LaB6 e-gun assy with W ... I have
just re-installed the LaB6 (currently pumping down), and have inspected the
W assy which wasn't working. It is definitely open, but I wondering if what
I'm seeing isn't as much an answer but rather a clue. That is, the W
filament appears to have been "zapped" ... open with beads on both ends.
Could the LaB6 configuration have put too much current through the W
filament?? TIA (again) ...

cheers, shaf
{\/} /\ {\/} /\ {\/} /\ {\/} cognito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/}
Michael Shaffer, R.A. - University of Oregon Electron Probe Facility
mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu
http://darkwing.uoregon.edu/~mshaf/epmahome/





From: Paul Webster :      paul.webster-at-yale.edu
Date: 14 May 1996 18:07:48 -0400
Subject: LM- Stereoscope by Wild-Hee

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Message-Id: {n1380025000.18876-at-QuickMail.Yale.edu}

If I remember correctly "Wild-Heerburg" became "Wild-Leitz", which then became
"Leica".

The number for Leica is 1-800-248-0123.

Let us know if they still supply parts for all their old microscopes.

Best regards,

Paul Webster
Center for Cell Imaging
Yale University School of Medicine
http://info.med.yale.edu/cellimg





From: Scott Holt :      102467.2752-at-CompuServe.COM
Date: 14 May 96 10:18:44 EDT
Subject: BUEHLER:Polishing Gold

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Robert McDonald requested information on polishing gold by hand or with basic
equipment. My suggestion is to perform your final, deformation removing step,
on a soft cloth such as BUEHLER's MICROCLOTH(R) or MASTERTEX(R).
Instead of 0.25micron diamond, use MASTERMET(R) final polishing suspension
with light pressure to polish the gold surface.

The ideal is to perform final polishing on a vibratory polisher such as
BUEHLER's
VIBROMET(R) 2. Recent years have seen a great change in vibratory polishing
at BUEHLER. In the past, vibratory polishing was used sparingly by many people
due to the rounding effects incurred. However, the VIBROMET(R) 2 has been
changed to produce less of a vertical movement component, and to increase the
horizontal component. This effectively eliminates the rounding effects which
were
the bane of vibratory polishing in the past.

If you have any further questions regarding gold polishing or vibratory
polishing,
please don't hesitate to contact me (I will be out for the remainder of the
week), or
one of our other sample preparation laboratory experts by calling (800)BUEHLER
[(800)283-4537]. Or if you would rather contact us by FAX: (847)295-7942.

Best regards,
Scott D. Holt
BUEHLER
41 Waukegan Rd.
Lake Bluff, IL 60044
(847)295-4546
http://www.buehlerltd.com





From: shaf :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 14 May 1996 08:12:33 -0700
Subject: JEOL 840|6300 users

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Message-Id: {2.2.32.19960514151233.006ac000-at-darkwing.uoregon.edu}
X-Sender: mshaf-at-darkwing.uoregon.edu
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I have removed my LaB6 assembly from our JSM-6300 for cleaning, and wanted
to replace it with, and use, the W assy in the mean time. The trouble is the
HV heat indicate some amount of the "preheat" which is for the LaB6 but I
can't put any more current thru the filament than that ... and the emission
implies zero. The logical thing to check is if the filament is actually open
but it should be good.

It is my impression in spite of the LaB6 demanding "preheat", I should be
able to interchange both gun assemblies. Is there something which is
associated with the LaB6 configuration that I am missing??

Also: in spite of you 840|6300 users not be able to help, please respond
anyway (directly ... see "oregon" address below) ... I'd like to create an
e-mail group which is a bit more specific to my "microscopy" applications.
TIA ...

cheers, shaf
{\/} /\ {\/} /\ {\/} /\ {\/} cognito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/}
Michael Shaffer, R.A. - University of Oregon Electron Probe Facility
mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu
http://darkwing.uoregon.edu/~mshaf/epmahome/





From: Nina S Allen :      nallen-at-unity.ncsu.edu
Date: Tue, 14 May 1996 19:08:47 -0400 (EDT)
Subject: Re: High-quality overheads from EM micrographs

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We have a FuJix color printer that does transparencies very well. It is
not cheap and the transpareancy paper itself is ca. $3.0o a page. The
Codonics printers also do a good job. They are ca. $9000. Good luck.
Nina allen

On Tue, 14 May 1996, Roy Christoffersen wrote:

} Does anyone out there know how to make high-quality overheads from standard
} B&W photographs (e.g., TEM images including HRTEM images)? I have always
} made slides for my talks up until now. Just using the standard xerox
} transparencies does not give very good results, but I have seen talks where
} people use transparencies and they are remarkably good. Is there some trick
} or method I am missing out on?
}
} Roy Christoffersen
} Texas Center for Superconductivity
} 3201 Cullen
} Houston, TX 77204-5932
} roy-at-bayou.uh.edu
} (713) 743-8273
} FAX: (713) 743-2787
}
}
}




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Tue, 14 May 1996 08:54:04 GMT
Subject: Re: Spinning solutions for immunogold

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Message-Id: {199605141725.MAA08862-at-Sparc5.Microscopy.Com}

} Does anyone have an idea how long and how fast to spin IgG gold
} solutions and BSA to help reduce background on en-grid imunogold
} labelling.
}
} I am specifically looking at p450 enzyme in lung tissue but am having
} a bit of difficulty with high backgroud in my controls.
}
} Thanks alot
}
} Ray Gilbert
} University of Leicester
} e-mail rtg2-at-le.ac.uk
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

Depends on the size of the gold. we spin 15-20 nm gold one minute at top
speed on a microfuge. As our IgG gold gets older a small pellet will form.
New stuff rarely has a pellet
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Tue, 14 May 1996 08:29:46 -0600
Subject: Re: Two mab labeling

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Dear Paul Webster,
I did not see the messages that you were talking about.
But I remember reading about this in an article by A.C. Cuello in the book
Immunochemistry II, that he/she edited, published in 1993, by Wiley, in
their "IBRO Handbook Series: Methods in the Neurosciences (General editor
A.D.Smith) Volume 14. The book is mostly about how to follow neurons around
in huge volumes of brain, but Cuello has an article that deals with
multiple mab labelling. If I remember, the method required direct
conjugation of different dyes to the different mabs. This sounds a bit
tedious, but then it has the delightful advantage of elimanating hassles
with crossreacting secondaries. I have no personal experience with the
method.

Cheers,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: Stephen Edgar :      s.edgar-at-auckland.ac.nz
Date: Tue, 14 May 1996 15:00:27 NZS
Subject: Re: Osmium in "good" buffers

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Here is a brief summary of the responses to my question of last week:

} has anyone
} besides me had problems with osmium made up in PIPES?

I am not sure whether their responses went to the List or just to
me, but my thanks to Rosemary White, Tom Phillips, Larry Oakford,
and John Heckman for their comprehensive replies. Sorry I don't have
the time to respond to each of you separately.

Rosemary suggested the problem was with the NaOH used to adjust the
pH of the PIPES, which makes sense since that was what I used. She
recommended KOH-PIPES or a mix of MES & PIPES instead.

Larry considered Veronal buffer to be the most appropriate buffer for
OsO4, but the majority opinion (LO, TP, JH) was that unbuffered
osmium works at least as well as buffered osmium.

Coincidentally, I discovered that our trainee technician recently made
up our osmium unbuffered .... and no-one noticed. (Don't tell our
Linda though!)


Regards

Stephen Edgar

Electron Microscope Unit, Pathology Department
School of Medicine
University of Auckland
Private Bag 92019
Auckland
New Zealand

email address: s.edgar-at-auckland.ac.nz
Phone : +64-9-3737599 extn 6473 (GMT + 12h)
Fax : +64-9-3737459




From: Mark Yeadon :      yeadon-at-uimrl7.mrl.uiuc.edu
Date: Tue, 14 May 1996 14:20:26 -0600 (CST)
Subject: Re: High-quality overheads from EM micrographs

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Dear Roy,

The main methods of producing high-quality EM viewgraphs that I've come
across are:

1. Going to a Copyshop that has a Laser Photocopier, viewgraph ready for
photocopying, and having it lasercopied onto transparency. The results are
remarkably good in my experience; because the laser-copier prints digitally
you can also photocopy a laser-copy with results far better than a photocopy
of the original (e.g. for hand-outs from your talk etc.).

2. We obtain very nice results by scanning the original EM negative into a
PC using an AGFA Arcus II flatbed scanner; the picture files are then
inverted (to get a positive), imported into MS Powerpoint and incorporated
in viewgraphs that way. The scanner is used in 'transmission mode' for
negatives. If you have a scanner without the transmission feature, scanning
a print works almost as well for viewgraphs.

Best Wishes,

Mark Yeadon

----------------------------------------------------------------------

Mark Yeadon
Materials Research Laboratory
University of Illinois at Urbana-Champaign
Urbana
Illinois 61801, USA

Tel: (217) 333 2514
Fax: (217) 244 2278

email: yeadon-at-uimrl7.mrl.uiuc.edu
----------------------------------------------------------------------





From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Tue, 14 May 1996 11:10:23 GMT
Subject: Message Undeliverable to X.400

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} } Does anyone have an idea how long and how fast to spin IgG gold
} } solutions and BSA to help reduce background on en-grid imunogold
} } labelling.
} }
} } I am specifically looking at p450 enzyme in lung tissue but am having
} } a bit of difficulty with high backgroud in my controls.
} }
} } Thanks alot
} }
} } Ray Gilbert
} } University of Leicester
} } e-mail rtg2-at-le.ac.uk
} }
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
}
} Depends on the size of the gold. we spin 15-20 nm gold one minute at top
} speed on a microfuge. As our IgG gold gets older a small pellet will form.
} New stuff rarely has a pellet
} *******************************************************
} Greg Erdos Phone: 352-392-1295
} Scientific Director,
} ICBR Electron Microscopy Core Lab
} 218 Carr Hall Fax: 352-846-0251
} University of Florida E-mail: gwe-at-biotech.ufl.edu
} Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/
}
} *******************************************************
}
}
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: Luc Nocente :      ln-at-noesisvision.com
Date: Tue, 14 May 1996 09:09:48 -0400
Subject: Re:

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Joe Michael writes,
} Dear Colleagues,
}
} There is an excellent reference on the Hough Transform that covers both the
} extraction of linear features and circular features. The title is
}
} "Shape Detection in Computer Vision Using the Hough Transform"
}
} by V. F. Leavers
} Springer-Verlag
} New York

For your information, Visilog from Noesis Vision Inc provides a module for
doing Hough Transform and Correlation. You can extract linear or circular
features using these algorithms but also predefined shapes in an image using
a model of the shape.

----------------------------------------------------------------------------
---------------------
Luc Nocente Tel: 514 345 1400
Noesis Vision Inc. Fax: 514 345 1575
e-mail: ln-at-noesisvision.com http://www.cam.org/~noesis
6800 Cote de Liesse, Suite 200
St-Laurent, PQ
H4T 2A7,Canada

Join the Noesis NewsGroup at Visilog-at-noesisvision.com
----------------------------------------------------------------------------
---------------------





From: (Ya Chen) :      ychen-at-MACC.WISC.EDU
Date: Tue, 14 May 1996 14:20:40 -0700
Subject: Re: High-quality overheads from EM micrographs

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Message-Id: {v02120d00adbea7f3822a-at-[144.92.132.31]}

} Date: Tue, 14 May 1996 08:53:53 -0500
} From: (Roy Christoffersen) {roy-at-bayou.uh.edu}
} Subject: High-quality overheads from EM micrographs
} To: Microscopy-at-sparc5.microscopy.com
} MIME-version: 1.0
} Content-type: text/plain; charset="us-ascii"
} Content-transfer-encoding: 7BIT
}
} Does anyone out there know how to make high-quality overheads from standard
} B&W photographs (e.g., TEM images including HRTEM images)? I have always
} made slides for my talks up until now. Just using the standard xerox
} transparencies does not give very good results, but I have seen talks where
} people use transparencies and they are remarkably good. Is there some trick
} or method I am missing out on?
}
} Roy Christoffersen
} Texas Center for Superconductivity
} 3201 Cullen
} Houston, TX 77204-5932
} roy-at-bayou.uh.edu
} (713) 743-8273
} FAX: (713) 743-2787

Roy,

You can digitize you EM images, import into PageMaker, export to high
quality laser printer (} 1200dpi), and print on transparencies directly.
The quality is much better than from xerox. Good luck,

Ya Chen


Ya Chen

==========================================================================
Cryo/SEM Coordinator
Integrated Microscopy Resource (IMR)-- III M M RRRRRR
an NIH Biomedical Research Resource I M M M M R R
University of Wisconsin, Madison, WI I M M M RRRRRR
1675 Observatory Drive #167 I M M R R
Madison, WI 53706, USA I M M R R
TEL : 608-263-8481 I M M R R
FAX : 608-265-4076 III M M R R
Email:YChen-at-macc.wisc.edu
==========================================================================
IMR WWW Home Page: http://www.bocklabs.wisc.edu/imr.html
2nd Symposium on Integrated Microscopy: Sept. 20-22, 1996






From: IAN HALLETT :      ihallett-at-MARCCRI.MARC.CRI.NZ
Date: Wed, 15 May 1996 10:37:29 GMT+1200
Subject: Live/Dead cells - Summary

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Thank you to everyone who replied to my query on differentiation live
and dead plant cells.

The approaches fell into two categories.

Firstly those detecting membrane integrity using dye exclusion (eg
Trypan Blue) sometimes with a stain such as Neutral Red that is taken
up by the live cells.

Secondly those detecting enzyme activity in the living cells,
particularly using fluoroscein diacetate (non-fluorescent) which is split
by esterases in living cells an then fluoresces. Example references

The Heslop-Harrison & Heslop-Harrison (1970) Stain Tech. vol 45 pp 115-
120 - pollen. For a tissue culture reference see Widholm, J.M. (1972) Stain
Tech. vol47 pp 189-194. Harris, N and Oparka, K - Plant
Cell Biology, A practical approach (1994) IRL Press

A modification of this second is using propidium iodide to penetrate
dead cells - (live green fluorescence, dead red - using blue excitation)

In a similar vein the use of LIVE/DEAD kits from Molecular Probes was
suggested by several people though these are not designed for plant
cells.

We have carried out an initial test using fluoroscein diacetate which
has worked in our experimental system, though there are some problems
with clumping of the cells.

Once again thanks to everyone

Ian








Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660
EMail ihallett-at-hort.cri.nz




From: Doug Keene :      DRK-at-shcc.org
Date: Mon, 13 May 1996 18:11:34 -0800 (PST)
Subject: subscribe

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Subscribe, please.




From: IAN HALLETT :      ihallett-at-MARCCRI.MARC.CRI.NZ
Date: Wed, 15 May 1996 14:34:32 GMT+1200
Subject: Live/Dead cells - Summary

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {n1380108162.44079-at-QuickMail.Yale.edu}


Thank you to everyone who replied to my query on differentiation live
and dead plant cells.

The approaches fell into two categories.

Firstly those detecting membrane integrity using dye exclusion (eg
Trypan Blue) sometimes with a stain such as Neutral Red that is taken
up by the live cells.

Secondly those detecting enzyme activity in the living cells,
particularly using fluoroscein diacetate (non-fluorescent) which is
split by esterases in living cells an then fluoresces. Example
references

The Heslop-Harrison & Heslop-Harrison (1970) Stain Tech. vol 45 pp
115- 120 - pollen. For a tissue culture reference see Widholm, J.M.
(1972) Stain Tech. vol47 pp 189-194. Harris, N and Oparka, K - Plant
Cell Biology, A practical approach (1994) IRL Press

A modification of this second is using propidium iodide to penetrate
dead cells - (live green fluorescence, dead red - using blue
excitation)

In a similar vein the use of LIVE/DEAD kits from Molecular Probes was
suggested by several people though these are not designed for plant
cells.

We have carried out an initial test using fluoroscein diacetate which
has worked in our experimental system, though there are some problems
with clumping of the cells.

Once again thanks to everyone

Ian








Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660
EMail ihallett-at-hort.cri.nz




From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Wed, 15 May 1996 10:20:05 +1100
Subject: Re: spinning solutions for immunogold labelling

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A high background is unlikely to be fixed by centrifugation. Rather look at
your method. Possible causes include (partly taken from BioCell's Gold
Conjugates Technical Information and Guidelines booklet, partly personal
experience and partly a selection of papers):

..Ionic concentration too low. Use increased salt concentration, up to 30%.
..Inadequate washing.
..Nonspecific charge attraction of antibody. Use detergent (I use 0.4%
saponin; Tween is OK). Include normal serum (from the species the gold
conjugate came from) at 2% and cold water fish gelatin at 1% in antibody
solutions and at 10% and 1% respectively in the preincubation solution.
..Free aldehyde groups in tissue. Float sections on 0.5M NH4Cl for 1 hour
before incubation.
..Antibody concentration too high.
..Gold conjugate concentration too high.
..Tissue poorly fixed. Damaged cells stain nonspecifically.

Is the background on particular cell structures (eg nuclei), is it all over
the tissue containing area, or is it on the whole section (including resin
only areas)? Look at this first. Is the antibody or gold conjugate OK or
has it been around too long? Is there any bacterial contamination? What
sort of water are you using? Has the method worked before? Has the weather
changed recently; were you in a bad mood; was the moon full? Good luck.

Diana van Driel
Dept Ophthalmology
Sydney University
AUSTRALIA 2006






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Tue, 14 May 1996 21:52:00 -0800
Subject: Re: Overheads from EM transparencies

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Dear Roy,

I have had some reasonable results printing digital images onto overhead
transparencies on a 600 dpi laser printer. You should scan the images in to
at least 2000 by 2000 pixel resolution. The quality depends upon the
original digital image and the printer, so the better video printers will
do a better job. Also, some copiers have a photo copying option
(gray-scale) that will reproduce mid-tones.

However, I don't think an overhead transparency will ever be as good as a slide.
Regards,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Stephan Coetzee :      stephan-at-gecko.biol.wits.ac.za
Date: Wed, 15 May 1996 11:58:22 GMT+2
Subject: (Fwd) Re: Polishing Gold

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} Hi All in Microland:
}
} Does anyone have any tips for polishing gold to use as a standard?
}
} I have available only very basic polishing equipment which generally
} serves our needs for polished rock sections very well using diamond
} paste of varying grades.
}
} My problem seems to be that the gold is so soft that when I jump from
} one micron diamond to 0.25 I only make things worse.
}
} Any advice would be most welcome.
}

Dear Robert

The cloth is important as mentioned already as pressure. Having
absolutely clean uncontaminated cloths are important as well as moving
the sample in a spiral movement on the cloth in order to ensure a
even distribution of the diamonds. To prevent cross contamination
there should not be gap between the sample and the mould. Araldite
is brilliant and is available in most EM labs. Reasonable stable
under the beam when slightly C-coated. To ultrasonic clean the
sample between cloths with sample face down is also a plus to prevent
cross contamination. For final polish of 0.25 micron I had better
luck with Cerenium Oxide than with diamond.
Stephan H Coeztee
Electron Microscope Unit
Private Bag 3
Wits
2050
South Africa

Stephan-at-Gecko.biol.WITS.ac.za

Tell: +27 11 716 2419
Fax : +27 11 339 3407




From: CHAFFEYN :      NIGEL.CHAFFEY-at-bbsrc.ac.uk
Date: Wed, 15 May 1996 09:02:18 +0000
Subject: Bubbly methacrylate - cured (?)

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Via: uk.ac.bbsrc; Wed, 15 May 1996 09:16:42 +0100
X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; Relayed;
Wed, 15 May 1996 09:17:49 +0000
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Wed, 15 May 1996 09:02:18 +0000
Receipt Notification Requested) (IPM Return Requested)

Dear Fellow Microscopists,

Just a short note to thank all those who kindly replied to my original
query about problems with bubble formation in methacrylate. I have received
lots of ideas of ways to overcome the problem: I will be trying them when I
have some more plant material ready. In the meantime, it is always comforting
to know that there are people out there willing to share their knowledge. 'A
problem shared, is a problem halved, quartered, etc...'

Thank you,

Nigel

-------------------------------------------------------------------------------
Internet: nigel.chaffey-at-bbsrc.ac.uk IACR-LARS, Dept. of Agricultural
X400:G=nigel; S=chaffey; O=bbsrc; P=uk; C=GB Sciences, University of Bristol,
Tel: +44 (0)1275-392181 ext:230 Long Ashton Research Station,
Fax: +44 (0)1275-394281 Long Ashton, Bristol, BS18 9AF
-------------------------------------------------------------------------------




From: Paul Webster :      paul.webster-at-yale.edu
Date: 15 May 1996 09:36:28 -0400
Subject: Re: spinning solutions for i

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Message-Id: {n1379969277.66219-at-QuickMail.Yale.edu}

Diana van Driel writes

"A high background is unlikely to be fixed by centrifugation."

Unfortunately, we have just discovered that ultra-high centrifugation of some
antibodies prior to labeling, can remove non-specific binding on sections. I
have no explanation for this and offer my apologies for complicating the field
further.

There is a section on our CCI home page concerning problems with antibody
labeling if anyone is interested.

Thank you to all those who re-posted the information on muliple antibody
labeling protocols using monoclonal antibodies.

Paul Webster
Center for Cell Imaging
Yale University School of Medicine
htt://info.med.yale.edu/cellimg







From: lizard-at-okway.okstate.edu (Ginger Baker)
Date: Wed, 15 May 1996 08:40:52 -0500
Subject: microtomes

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Mime-Version: 1.0

Thank you for the information concerning the different types of
microtomes. My colleague much appreciates it.

Sincerely,

Ginger R. Baker
EM Lab Manager
Dept. of Anatomy, Pathology, and Pharmacology
250 Veterinary Medicine
Oklahoma State University
Stillwater, OK 74078
(405) 744-6765
FAX: (405) 744-5275




From: lizard-at-okway.okstate.edu (Ginger Baker)
Date: Wed, 15 May 1996 08:40:52 -0500
Subject: microtomes

Contents Retrieved from Microscopy Listserver Archives
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Mime-Version: 1.0

Thank you for the information concerning the different types of
microtomes. My colleague much appreciates it.

Sincerely,

Ginger R. Baker
EM Lab Manager
Dept. of Anatomy, Pathology, and Pharmacology
250 Veterinary Medicine
Oklahoma State University
Stillwater, OK 74078
(405) 744-6765
FAX: (405) 744-5275




From: becks-at-sunynassau.edu (Steve Beck)
Date: Wed, 15 May 1996 10:21:01 -0500
Subject: Digitizers/Frame Grabbers

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Dear Microscopists:

I am seeking information on the requirements to digitize our Hitachi
S-2400 SEM. We would like to digitally capture, archive (on CD-R, etc.),
and analyze images on a Power Mac. Can anyone recommend a good frame
grabber interface? Would we need any other equipment/hardware? I would also
appreciate any price information as we need this for a budget revision on a
pending NSF grant, which is due in a couple of days.

Thanks in advance.


Stephen Beck
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829





From: yimei-at-befvax.uchicago.edu
Date: Wed, 15 May 1996 11:19:00 EDT
Subject: staining advice

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{I have made a diblock copolymer and would like to investigate microphase
separation behavior in this material. One of the blocks is PMMA while the
other one is a linear conjugated polyene. I think osmium tetroxide is a good
first choice but I need some advice as to the exact way to stain. I already
have the samples in the grids. Do I stain with the vapor from an aqueous
solution or do I put the grids directly into the solution? for how long?
Please reply to tmmaddux-at-midway.uchicago.edu
Phone 312-702-1147
Todd MADDUX





From: yimei-at-befvax.uchicago.edu
Date: Wed, 15 May 1996 11:38:07 EDT
Subject: TEM, DIBLOCK COPOLYMER STAINING

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I have made a diblock copolymer and would like to investigate its
microphase separation behavior using TEM. One block is PMMA while the other
is a linear conjugated polyene. I think osmium tetroxide is a good first
choice for staining but I really don't know how to go about it. The sample
is already on the grids. Should I expose the grids to the vapor from an
aqueous solution or should I place the grids directly in the aqueous solution.
For how long?
Please reply to TMMADDUX-at-MIDWAY.UCHICAGO.EDU





From: Rebecca_Ai-RP3478-at-email.sps.mot.com
Date: 15 May 96 08:46:00 -0500
Subject: TEM Image of Dopant Profile

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Has anybody tried staining a TEM sample to image the dopant profile? Any
suggestions?


Rebecca
=========







From: Mary Anton :      semlab-at-mail.ims.uconn.edu
Date: Wed, 15 May 1996 09:33:42 -0400
Subject: SEM Users' Questions

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Hi,

I am looking into the purchase of a new conventional (W/LaB6) SEM for a
Materials Science laboratory and had a couple of questions for users of
newer SEM equipment:
1. I was told that LaB6 doesn't buy much of a brightness improvement over W
and therefore isn't worth the added expense, is this your experience?
2. With digital imaging as the fast increasing method for documentation, is
the record/film option still necessary?

All inputs are greatly appreciated as well as any other comments along these
lines. Thanks in advance.

Mary Anton
University of Connecticut
USA





From: Radice-at-urvax.urich.edu (Gary Radice)
Date: Wed, 15 May 1996 16:33:07 -0400
Subject: Scanning histological slides

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I believe there were some recent posts about capturing images of microscope
slides on a scanner. Unfortunately I did not save them but now I find that
this might be just what we need to do. Could someone briefly summarize what
was learned and respond either on the list or to me privately?

Gary Radice 804-289-8107 (voice)
Department of Biology 804-289-8233 (FAX)
University of Richmond
Richmond VA 23173
USA






From: LeeDMLM-at-aol.com
Date: Wed, 15 May 1996 17:14:37 -0400
Subject: Re: High-quality overheads from EM micrographs

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Roy

Hi! If you are making overheads from negatives, Ted Pella has a Dry Silver
Processor made by 3M that makes excellent overheads. You expose the negative
on the overhead transparency on the enlarger the same as if you were printing
the negative. It then takes 6 seconds to run it thru the processor for the
finished product. The printer sells for $2150. The overhead transparency
film costs about $2 apiece.

Lee Dickey, Micro Lines Marketing
Representing: Ted Pella Inc., RMC Inc., Denton Vacuum, Durst, and PGT




From: Linda Fox :      lfox1-at-wpo.it.luc.edu
Date: Wed, 15 May 1996 16:49:23 -0500
Subject: increase immuno gold staining

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Message-Id: {s19a0a7b.071-at-wpo.it.luc.edu}
X-Mailer: Novell GroupWise 4.1

I'm back again! Thanks for all the previous help. I love this
place!!

Today's problem involves a way to increase gold staining. I have
tissues freshly fixed in 2% PFA (also long term fixed in PLP) and run
up in both LRWhite and Lowicryl K4M. Can these resins be etched to
reveal more structure? Does the antibody and gold label get much
below the surface anyway?
I have increased time in primary to 24hrs. and don't get much
staining. The P.I. on this project has great LM under fluorescence
so we know that the antibody works. I am working with a kit from E.Y.
Laboratories for the other solutions. Any and all help is deeply
appreciated.

Linda Fox -at-wpo.it.luc.edu





From: Neal Nicklaus :      nnicklaus-at-nova.sarnoff.com
Date: Wed, 15 May 1996 11:51:01 -0400
Subject: Adding Confocality to a Microscope

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Message-Id: {199605152025.PAA12763-at-Sparc5.Microscopy.Com}

Does anyone know who makes attachments/accessories which add confocality to
existing microscopes?

I have read that a spinning disk with pinholes may be used at the focus of a
160 mm conjugated microscope lens to provide a confocal arrangement that can
work with a camera at video rates.

I have a home-built inverted microscope and want to add confocality, but
require video rates (I use an image intensified camera to look a
fluorescently tagged DNA in solution in real-time).



Neal Nicklaus

SEQ Limited

Voice: 609-452-6033 Ext. 13
Fax: 609-452-5955
email nnicklaus-at-seq.sarnoff.com





From: cxb41-at-po.CWRU.Edu (Christine H. Block)
Date: Wed, 15 May 1996 13:22:14 -0400
Subject: RMC ultramicrotomes

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I would like to thank everyone who responded to my question
about the MT 7000 ultramicrotomes.

I got a number of responses that ranged from very happy to very
dissatisfied.

In the interim, I have been in contact with RMC and they have
been extremely helpful in trouble-shooting the problems and
following up with service. Specifically, I would like to
thank Mr. Greg Carter and Mr. Tom Kennedy, who both have
given me considerable attention and assured me that my problems
with these instruments will be resolved.
We just need to wait a bit now, for parts..

I am finding this forum quite useful to solve issues that
probably occur in several labs. Thanks again!


Christine H. Block, Ph.D.
VA Medical Center
Cleveland, OH

--
_____
|\___\
|| |
\|___| mow down MO'TOWN--GO TRIBE!




From: waheeschen-at-dow.com (Bill Heeschen 517-636-4005 Materials/ASL)
Date: Wed, 15 May 1996 12:51:47 -0400
Subject: Re: Digitizers/Frame Grabbers

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Message-Id: {199605151651.AA16673-at-na3.dow.com}

To Stephen Beck:

First assumption: your Hitachi 'scope has a conventional video out (either
NTSC or PAL) port. If that assumption is invalid, contact 4pi analysis
about a full digital interface for running the 'scope.

The Scion LG-3 frame grabber is about $900 and does an excellent job
digitizing images with the NIH Image software. One caveat - make sure you
have a full-size (12") PCI or NuBus slot in your PowerMac (the 6100, for
example, has a 7" slot). Be sure to specify video format (US video =
NTSC/RS-170, European video = PAL/CCIR).

We use both systems regularly and are pleased with the performance.

Bill
==========================
Bill Heeschen/Analyt. Sci.- Mat. Char. -----
1897-F Building / The Dow Chemical Co. --- ---
Midland, MI 48667 U.S.A. --- D O W ---
phone: (517)636-4005 fax: (517)636-5453 --- ---
Email: waheeschen-at-dow.com -----
========================== R


Scion Corporation (frame grabber)
152 West Patrick St.
Frederick, MD 21701

voice: 301-695-7870
fax: 301-695-0035


4pi Analysis (digital EM control and EDS capture)
3500 Westgate Dr
Durham, NC 27707-2534

voice: 919-489-1757
fax: 919-489-1487




From: A. Greene :      ablue-at-mail.io.com
Date: Wed, 15 May 1996 11:10:08 -0500 (CDT)
Subject: Technical Info.

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Hello, I am trying to bring an old ISI SX-30 Scanning Electron Microscope
back to usable condition and have great difficulty trying to find good
documentation and usable schematic diagrams. I realize this is an old
instrument and maybe not exactly wonderful when it was at it's best. Topcon
Technologies (the new owners of ISI) have been contacted but offer little
help, even though we were willing to pay for the necessary documents. One
thing we did buy was what appears to be about a 3rd or 4th generation Xerox
copy of the system schematics.
It is very difficult to read. Any help would be greatly appreciated.

Thanks. Alex Greene
Austin, Texas





From: Smith, Peter :      SMithP-at-agresearch.cri.nz (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Wed, 15 May 1996 21:09:49 -0500
Subject: Anti Fade Compounds

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I would appreciate any feedback from people using fluorecsent antifade
compounds,such as DABCO,p-phenylenediamine or commercial kits such as
slowfade , prolong, citifluor etc.Being relatively new to the wonderful
world of fluorescent microscopy, I'd like to know how good they are,and are
they easy to use. Thanks

Peter Smith







From: Karen Vaughn :      klv-at-biotech.ufl.edu (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Wed, 15 May 1996 21:10:24 -0500
Subject: embedding insect eggs

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We are processing insect eggs for TEM and SEM. The egg is covered with a
impermeable surface that does not allow for the transfer of solutions and
especially resins. One attempt has been made following the standard protocol
which resulted in a full collapse of the sample. Cutting a slit in the egg
is not possible because the inside flushes out. Does anyone know of a method
for making this surface permeable?

Thank you in advance


----------------------------------------------------------------------------
---------
Karen Vaughn Tel.(904) 392-1184
EM Technician
University of Florida Fax.(904) 846-0251
Electron Microscopy Core Laboratory Email. KLV-at-biotech.ufl.edu
Interdisciplinary Center for Biotechnology Research
http://www.biotech.ufl.edu/~emcl/
214 Bartram Hall
Gainesville, Fl 32611










From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Wed, 15 May 1996 18:39:28 -0500
Subject: Free Access to Differential Contrast (Hysteresis) Software

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Message-Id: {v01520d02ada14f3d49b4-at-[155.37.2.10]}
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I got several responses to previous BB postings asking how to get access to
our differential contrast (hysteresis) imaging software (detailed in MSA
and MAS 95, 96, Scanning 1996, and my WWW pages).

We now have for distribution a software version for Silicon Graphics
Workstations (using SGI libraries) for 8-16 bit graytone, 24-bit RGB color
(both as TIFF), and unlimited image sizes. Since differential hysteresis
processing is very computer intensive, we are developing an option for
networking several SGI workstation for image processing through
distributed computing. A single SGI Indigo2 will need ca. 15 min. for a
8-16 bit 1Kx1K image, graytone or color. Smaller images require fractional
time (256x256 24-bit Color Tiff less than a min.).

The software is described at: http://panda.uchc.edu/htklaus/DHP-Img.html;
graytone applications are at: http://panda.uchc.edu/htklaus/Microsc-Img.html;
24-bit color application are at:
http://panda.uchc.edu/htklaus/Color/Color-Intro.html.

If you like to use this software please use the form provided at
http://panda.uchc.edu/htklaus/DHP-Img.html (if you do not have web access
please let me know by return email). You will need a SGI workstation, you
must work at an academic institution, and you will have to sign an
University license for non-distribution and non-commercial use, because my
University patented the technology. However, the processing results--I am
sure--will be an adequate reimbursement for the licensing inconvenience.

Also, we are putting the software on the web for unrestricted interactive
image processing (will be available in a few months).

Best regards Klaus.


******************************************************************************
* : *
* Klaus-Ruediger Peters, Ph.D. : WWW Pages: *
* Director, Molecular Imaging Laboratgory : *
* Biomolecular Structure Analysis Center : Molecular Imaging Laboratory *
* University of Connecticut Health Center : http://panda.uchc.edu/ *
* 263 Farmington Ave. : htklaus/index.html *
* Farmington, CT 06030-2017; U.S.A : Differential Color Imaging *
* : http://panda.uchc.edu/ *
* Tel: (203) 679-3977; Fax: (203) 679-1989 : htklaus/Color/ *
* Color-Intro.html *
******************************************************************************







From: Doug Keene :      DRK-at-shcc.org
Date: Wed, 15 May 1996 15:17:16 -0800 (PST)
Subject: computer-aided 2-D measurements

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Does anyone know of a software package which will allow measurements of rotary
shadowed molecules traced on a digitizing tablet from TEM micrographs? We do
not want to take the time to scan the images into the computer, and DO want to
work with printss. The molecules are quite kinked, so measurements with a
loupe are problematic. At one time we had a Bioquant system, using a
digitizing tablet and an IBM PC XT, but the tablet is broken and no longer
available, and the software worked only with that tablet. It did everything we
wanted when it worked. It would be nice if the suggested software would work
on a IBM computer and an off the shelf digitizing tablet, though MAC based
software would also work. We already have NIH Image, but the act of scanning
hundreds of images into the computer is not very unattractive.

Thanks in advance for any advice,

Doug Keene
Shriners Hospital Research Unit




From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Wed, 15 May 1996 21:46:52 -0500
Subject: Blatant Advertising on the ListServer....

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Message-Id: {199605160243.VAA13661-at-Sparc5.Microscopy.Com}
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Colleagues....

Yes I have seen the recent rash blatant advertising on the listserver and have
sent messages to each of the offenders. Please be advised that none of these
posting came with my "approval" even though at least one was suggested by
it's leading
message. In addition 2 of these individuals are not subscribers to the
distribution
list, they were simply opportunists taking advantage of the Email system.
Unfortunately
after sending a rebutting message there is little I can do.

This is an unmoderated server and as such I see the messages at just about
the same time all of you do. Occasionally I get requests to review
postings and will continue to do so and advise on the appropriateness
of any posting. Many commerical subscribers take advantage of this and
you should all appreciate their co-operation.

Just as a refresher I will repost a portion of the Listserver "rules". I am
please
to say that the vast majority of you follow them quite well. As always,
thanks for your
help, it makes my life simplier and allows me to get to sleep earlier.


Cheers....Nestor
Your Friendly Neighborhood SysOp




-----------------------------------------------------------------

Here is the exerpt from the FAQ file. Please note the section about
Advertising...

-----------------------------------------------------------------



What can I post?
----------------

Basically any question/comment/observation
or general information/announcement which involves
microscopy and/or microanalysis. This list is
not moderated, so it is up to the users to
self-police themselves. I generally take an openminded
attitude toward questions. If in my opinion, it appears that
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I prefer to err on the safe side with most messages
and allow at least a modicum of generally. Clearly,
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If there is any question in your mind about
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Can I post an Announcement of a Job Opening or a Meeting?
---------------------------------------------------------

Yes that falls within the bounds of the subject of this list as long
as it is related to Microscopy/Microanalysis.

Can I post my Resume'?
---------------------

No. This forum was not created for that purpose.


Can I post an Advertisement?
----------------------------

No, that does not fit within the bounds of this discussion forum.

This listserver is not intended to be a Sales mechanism
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Over the last few years I have found that many users have their mail
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Are There Any Special Settings on my Email Program?
----------------------------------------------------

Yes.

1.) Please insure that your correct Email address
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2.) DONOT set your Email Program to automatically
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-------------------------------

Compose your message off-line, then read it a second time!

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From: Jouko =?iso-8859-1?Q?M=E4ki?= :      jokamaki-at-utu.fi
Date: Thu, 16 May 1996 13:43:00 +0300
Subject: Overheads from EM-negatives

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X-Sender: jokamaki-at-mailhost.utu.fi
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To: Microscopy-at-Sparc5.Microscopy.Com

Hello All,

I guess this might interest fairly many of you.
I have used the method which I learned in Japan, Sendai.
Juji makes a product called FUJIGRAPH PROJECTION FILM PT-100, which you can
get in A4-size.
The film can be used as enlarging paper and the results are very good.
They used this film in the MRI in Tohoku University, where Professor Hiraga
taught me to use it.

Regards,
Jouko

Jouko K. M=E4ki, Ph.D., Laboratory Manager
University of Turku, Laboratory of Electron Microscopy
Kiinamyllynkatu 10 FIN-20520 TURKU FINLAND
Tel: +358 21 333 7318 GSM: +358 40 505 2521 FAX: +358 21 333 7380





From: Jeremy.Sanderson-at-path.ox.ac.uk :      sanderson-at-molbiol.ox.ac.uk
Date: Thu, 16 May 1996 13:36:25 +0100
Subject: DAB intensification

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Dear all,
Please will you e-mail me protocols for DAB intensification using
transition metal salts if you use these methods. I have also put this
request out on the confocal listserver. For those of you on both
listservers I apologise for the duplication.Jeremy.Sanderson-at-path.ox.ac.uk
Jeremy Sanderson
Light & Electron Microscopy
Sir William Dunn School of Pathology
South Parks Road
Oxford
OX1 3RE
Tel: 44-1865 275539
Fax: 44-1865 275515
e-mail:Jeremy.Sanderson-at-path.ox.ac.uk






From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Thu, 16 May 1996 09:27:06 -0500
Subject: Re: Adding Confocality to a Microscope

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Message-ID: {319B3B3A.971-at-jagunet.com}

Neal Nicklaus wrote:
}
} Does anyone know who makes attachments/accessories which add confocality to
} existing microscopes?
}
} I have read that a spinning disk with pinholes may be used at the focus of a
} 160 mm conjugated microscope lens to provide a confocal arrangement that can
} work with a camera at video rates.
}
} I have a home-built inverted microscope and want to add confocality, but
} require video rates (I use an image intensified camera to look a
} fluorescently tagged DNA in solution in real-time).
}
} Neal Nicklaus
}
} SEQ Limited
}
} Voice: 609-452-6033 Ext. 13
} Fax: 609-452-5955
} email nnicklaus-at-seq.sarnoff.com


Dear Neal,

Nikon, Inc. markets the K2sBio (Nipkow Spinning Disk) Confocal
Attachment and is priced at about $16K. Unfortunately, this unit will
only work on Upright compound microscopes as it takes the place of a
traditional Epi-Fluorescence attachment. To solve your dilemma contact
Meridian Instruments at (800) 247-8084, they make a similar unit named
the Insight that works on most Inverted platforms.

Good Luck,

Lawrence Kordon
Nikon, Inc.
Columbia, Maryland
nikon-at-jagunet.com




From: levin-at-ecsuc.ctstateu.edu (Martin Levin)
Date: Thu, 16 May 1996 11:26:17 -0500
Subject: Need Sorval MT2B Ultramicrotome

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materials {materials-l-at-LIVERPOOL.AC.UK} ,
microscopy {Microscopy-at-Sparc5.Microscopy.Com}

A friend of mine will be offering an EM course at the University of
Hartford this fall. The course has not been run for a few years and he
finds that he is in need of an ultramicrotome (or two). As is the case
with all small colleges, there is not a great deal of money available. He
would like to spend as little as possible (free would be ideal) on a
ultramicrotome. Since he is most familiar with the Sorval MT2B, he was
hoping that someone out there might have one sitting in their lab, in
more-or-less working order, that he could have.

He does not have access to the internet, so he asked me if I would send
this message out. So, if there is anyone who has a MT2B and would not mind
parting with it, please Email me.

Thanks,
Marty Levin

Martin A. Levin
Department of Biology
Eastern Connecticut State University
Willimantic, CT 06226
Phone: (860)465-4324 FAX: (860)465-5213






From: RNBALDUC-at-ARCRIDE.EDU.AR
Date: Thu, 16 May 1996 16:21 -0300
Subject: TEM screen recoating

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Message-ID: {70689B3101734AD2-at-c2smtp.reliance.rockwell.com}

Dear microscopyst:
Please will you e-mail me protocols or technics for rebuild my old TEM screen
thanks in advance
F. Balducci




From: Smith, Peter :      SMithP-at-agresearch.cri.nz (by way of
Date: Wed, 15 May 1996 21:09:49 -0500
Subject: Anti Fade Compounds

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Message-Id: {n1379889483.61166-at-QuickMail.Yale.edu}
"Smith, Peter" {SMithP-at-agresearch.cri.nz}
X-Mailer: Mail*Link SMTP-QM 3.0.3 b1 d5

Reply to: RE} Anti Fade Compounds

Hello Peter,
I use the receipe of Christian Broesamle (tips and tricks in netscape)
The receipe is as follows:
6 g glycerol (analytical grad)
2,4 g mowiol 4-88 [Calbiochem #475904]
6 ml dd H2O
add 12 ml 0.2 M Tris buffer pH 8.5 and mix for half a day on shaker
let the mixture sit for 2h
incubate at 50 degrees centigrade for 10 min
centrifugate mixture at 5000g for 15'
aliquot and freeze supernatant at -20 C until use
add 0.1 % DABCO [Aldrich #D2.780-2]

This helps to reduce fading but still....
good luck
annette Bakker
--------------------------------------

Peter Smith




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From: NANCY SMITH :      NSMITH-at-darwin.sci.csuhayward.edu
Date: Thu, 16 May 1996 12:08:32 PSD8PDT
Subject: subscribe

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subscribe Nancy Crise Smith {nsmith-at-csuhayward.edu}




From: John Best :      jbest-at-vicon.net
Date: Thu, 16 May 1996 09:54:20 -0700
Subject: Re: SEM Users' Questions

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Message-ID: {319B5DBC.1BF6-at-vicon.net}

Mary Anton wrote:
Hi,
I am looking into the purchase of a new conventional (W/LaB6) SEM for a
Materials Science laboratory and had a couple of questions for users of
newer SEM equipment:
1. I was told that LaB6 doesn't buy much of a brightness improvement
over W and therefore isn't worth the added expense, is this your
experience?
2. With digital imaging as the fast increasing method for documentation,
is the record/film option still necessary?
All inputs are greatly appreciated as well as any other comments along
these lines. Thanks in advance.
Mary Anton University of Connecticut USA


My response...........
Hi Mary and all,

I'll throw my two cents in! Technical advantages of LaB6 aside, the
extended life is worth it, as you'll spend less time changing filaments.
To keep a W filament working at peak potential, you nees to spend a
significant amount of time keeping the anode, gun and column clean. Also
, depending on your SEM, you may need to assure that the W filament is
mechanically aligned for best performance. I also think that the W
filament is more stable once it's been in use for a while, which is an
advantage when doing ultra slow scans required for Xray mapping, SC
imaging and some BSE work.

WRT the Polaroid option: If your clients have PC's, I'd say don't get it.
Network the images to them and let them view them on the PC. If you
absolutely need a film copy at some point in the future, send your image
to a printer and have it printed on any media you wish. In my experience
a printout (or two) on an inexpensive 600 dpi printer can convey as much
information as a Polaroid if the image is carefully chosen. I'm not
saying the print quality is as good, but a low mag and possibly a high
mag view of the area of interest can convey as much information to the
user as a Polaroid.

Please keep in mind that my suggestions are based on a non-existent
knowledge about exactly what you'll be doing. In general, I think
materials people would agree with me, but I'm very interested in reading
their response.

Good luck, John Best -- ELMDAS Co.




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 16 May 1996 11:23:55 GMT
Subject: Service contracts

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The University of Florida is strongly urging all of us to drop our
service contracts on all types of equipment in favor of going with a company
called CIC Agency who will handle service payments. We chose the service
provider and they pay for the services charges. It sort of like an HMO. We
pay a fixed annual fee.

Has anyone had experience with CIC or similar? If so could you share with
us your feelings.
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: MCCLELLAN DAVID S :      MCCLELLAN_DAVID_S-at-lilly.com
Date: Thu, 16 May 1996 19:12:01 +0000 (GMT)
Subject: I would like to subscribe to the Microscopy Listserver. My email

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address is
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I would like to subscribe to the Microscopy Listserver. My email address is
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Thank you!

David McClellan





From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Thu, 16 May 1996 13:09:19 -0600
Subject: Used SEM Available

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I have been asked to post the following information to the microscopy list.
If you are interested, please contact the company directly.

JEOL JSM-35c (1978) SEM; 0-35 kV; 7.5 nm resolution; 100,000X
Kevex-Ray EDS Analyzer (1980) (model number not given)
Hummer V Sputter Coater

Available from:

METAL 7 INC
Sept-Iles Qc, CANADA
FAX (418) 962-4534
Voice (418) 968-5822

John
chandler-at-lamar.ColoState.EDU






From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Thu, 16 May 1996 09:44:57 -0500
Subject: MMS Spring Symposium Announcement

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Announcing the Annual
Minnesota Microscopy Society Annual SPRING SYMPOSIUM
***********************************************************

To be held at SHERATON INN, MIDWAY, I-94 at HAMLINE AVENUE, ST. PAUL MN
on
THURSDAY - MAY 23, 1996

***********************************************************
SCHEDULE OF EVENTS
8:00 - 9:00 am Coffee and Late Registration

9:00 - 9:45 am Understanding Video Signals: Cameras, Recording Formats and
Printers
MARTY HARALDSON, Alpha Video, Edina, MN

9:45 - 10:30 am Coffee Break \ Vendor Displays

10:30 - 11:15 am Capturing, Storing and Organizing Digital Image Files
MARK SANDERS, Imaging Center, College of Biological Sciences, University of
Minnesota, St.Paul Campus

11:15 - 12:45 pm A buffet lunch will be served at the Sheraton Inn

12:45 - 1:00 pm MMS Business Meeting and Election of Officers

1:00 - 1:45 pm Microscopy Resources on the Internet
Dr. STUART MCKERNAN, Center for Interfacial Engineering, Characterization
Facility, U of MN, East Bank Campus

1:45 - 2:15 pm Coffee Break \ Vendor Displays

2:15 - 3:00 pm Public Domain Shareware, Microscopy and the Internet
Dr. DAVID BRIGHT, 1996 MSA Traveling Speaker
Research Chemist, Microanalysis Group, NIST, MD

3:00 - 4:30 pm Vendor Displays

Please make your reservation in advance! NO LATER THAN MONDAY, MAY 20, if you
plan to attend the Symposium (or you can pay at the door if unavoidable).
Contact Stuart McKernan at (612) 626-7942, stuartm-at-maroon.tc.umn.edu or
Dwight Erickson at (612) 736-2830, usmmm214-at-ibmmail.com

Symposium Fee: $20.00 current regular MMS members 95/96, $10.00 student members
95/96, $30 non-member(confers regular membership), or $15.00 non-member students
(confers student membership).


Stuart McKernan stuartm-at-maroon.tc.umn.edu
CIE Microscopy Center, University of Minnesota Office: (612) 626-7942
100 Union Street S. E., Minneapolis, MN 55455 Lab: (612) 624-6590





From: Neal Nicklaus :      nnicklaus-at-nova.sarnoff.com
Date: Thu, 16 May 1996 10:52:49 -0400
Subject: Re: Adding Confocality to a Microscope

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Message-Id: {199605161453.KAA14180-at-vaxserv}
X-Sender: nnicklaus-at-cave.sarnoff.com
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Mime-Version: 1.0
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I thank everyone for the feedback. A brief summary is below. I'll send an
update as more info arrives.

1) Tracor Ltd in the USA making tandem confocals

2) Noran (http://www.noran.com/)

3) Meridian Instruments at (800) 247-8084, attachment for inverted platforms

4) Nikon, Inc. markets the K2sBio (Nipkow Spinning Disk) Confocal
Attachment and is priced at about $16K. Unfortunately, this unit will
only work on Upright compound microscopes

5) Possible new design with better light budget by Rimas Juskaitis' and Tony
Wilson's

6) Recommended book to read is: (Handbook of Biological Confocal
Microscopy, James B Pawley ed. 2nD edition, Plenum ISBN 0-306-44826-2)

---------------------------------------
At 12:13 AM 5/16/96 -0800, you wrote:
} } Does anyone know who makes attachments/accessories which add confocality to
} } existing microscopes?
} }
} } I have read that a spinning disk with pinholes may be used at the focus of a
} } 160 mm conjugated microscope lens to provide a confocal arrangement that can
} } work with a camera at video rates.
} }
} } I have a home-built inverted microscope and want to add confocality, but
} } require video rates (I use an image intensified camera to look a
} } fluorescently tagged DNA in solution in real-time).
} }
} }
} }
} } Neal Nicklaus
} }
} } SEQ Limited
} }
} } Voice: 609-452-6033 Ext. 13
} } Fax: 609-452-5955
} } email nnicklaus-at-seq.sarnoff.com
}
}
}
}
} Nick,
}
} I'd be quite grateful if you'd share your results with me.
}
} Thanks,
}
}
}
}
}
}
} Regards,
}
}
}
} (signed) Ed Monberg {em-at-mediacity.com}
}
} --------------------------------------------------
}
} 510-429-1060 Fax 429-1065
} LMDC, (Laser Motion Development Co.)
} 3101 Whipple Road
} Union City, CA 94587-1216
}
}
} For our Most recent Catalogue of "On Hand" EQUIPMENT:
} Send empty mail to: {Cat-at-lasermotion.com}
}
}
} Our web page: http://www.lasermotion.com (Is beginning to take shape!)
} Our e-mail: office-at-lasermotion.com
}
} {-------------------------------- Our page width
} -----------------------------}
}
}
}
}

Neal Nicklaus

SEQ Limited

Voice: 609-452-6033 Ext. 13
Fax: 609-452-5955
email nnicklaus-at-seq.sarnoff.com





From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Thu, 16 May 1996 17:18:32 -0600
Subject: re: SEM user's questions

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I'll answer just one of Mry Anton's questions, that regarding LaB6
cathodes. I tested LaB6 cathodes extensively on our Philips TEMs a few
years ago (reported in the 1994 MAS Proceedings). The result is that
you'll only see a factor of 3 improvement over W filaments if you buy LaB6
cathodes with a 90 degree cone angle. The 10x brightness which has been
touted for years only occurs for smaller cone angles (60 degrees). If one
actually looks at the original literature which is the source of the 10x
claim (J. Vac. Sci. Technol. 15(3)1978), the cone angle of the emitters is
less than 90 degrees.

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(708)252-7194
FAX: (708)252-4798






From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Thu, 16 May 1996 16:11:30 -0800
Subject: ergonomic keyboards

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howdy all

I'm spending more and more time at my keyboard doing images etc and find
myself suffering. Someone suggested the ergonomic keyboards have been a
real help, in addition to changing desks, layouts, foam pads etc. Anyone
have some pros and cons of these odd looking keyboards before I run out and
buy one? I have read several books in this regard, but would welcome any
other tips on reducing repetitive stress (my doctor, bless him, said dryly,
well, if it hurts to do it,....then don't do it....).

thanks

steve

Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego, CA 92182-4614
phone: (619)594-4523
fax:(619)594-5676
email:sbarlow-at-sunstroke.sdsu.edu






From: Elinor Solit :      cambrex-at-world.std.com
Date: Thu, 16 May 1996 15:52:22 -0400 (EDT)
Subject: Re: Used equipment suppliers

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Mr. Synder,

Carl Zeiss has just announced that they are selling their own
used equipment. What a chance to get some of those wonderful older
microscopes. Suggest you call them at 800-356-1090. Let me know how this
works out for you.

Regards,

Ellie Solit
The Microscope Book

On Thu, 16 May 1996, SNYDER, JOSEPH wrote:

} I am looking for sources of used equipment such as equipment brokers etc.
} Specifically the types of equipment include coating, microscopy and
} mechanical test equipment. Secondly I'm also trying to locate sources of
} discounted consumable supplies for metallurgical polishing work.
}
} I would deeply appreciate any leads.
}




From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Fri, 17 May 1996 10:58:57 -0500 (EST)
Subject: Re: TEM cooling system additives

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Dear Allan,

I use sodium borate, add enough to raise pH to about 9.0. Our chillers are on
a preventative maintainance schedule with our maintainance department and they
change the water. I just add the borate.

Best of luck
Ed Calomeni
Medical College of Ohio
Toledo, OH 43699
emlab-at-opus.mco.edu






From: Linda Fox :      lfox1-at-wpo.it.luc.edu
Date: Fri, 17 May 1996 10:27:19 -0500
Subject: RE: TEM cooling system additives

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The water in our recirculating unit was recently changed under our
yearly service contract. The engineer filled it with water from our
Milli-Q system and had us order one liter of Ethylene Glycol (Fisher
Scientific) to add to the system. I just checked, and there is
nothing growing, that I can see, however there is a faint metallic
sheen across surface of the water. Hope this helps.
Linda M. Fox lfox1-at-wpo.it.luc.edu





From: Rebecca_Ai-RP3478-at-email.sps.mot.com
Date: 17 May 96 08:14:00 -0500
Subject: TEM Image of Dopant Profile

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Thanks everybody for your helpful inputs.



Regards,
Rebecca




From: Joergen Bilde-Soerensen 5709 :      j.bilde-at-risoe.dk
Date: Fri, 17 May 1996 10:18:48 +0200
Subject: SEM Users' Questions

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Mary Anton wrote:

} I was told that LaB6 doesn't buy much of a brightness improvement
} over W and therefore isn't worth the added expense, is this your
} experience?

Hi Mary,

I fully agree with John Best's remark that "technical advantages of LaB6
aside, the extended life time is worth it". This becomes
particularly important if you are going to use automated procedures.
We upgraded our JEOL 840 microscope from W to LaB6 filaments because
we are often running automated overnight measurements of electron
back scattering patterns. With an average life time of 50 hrs for a W
filament, there is a probability of 1/3 that an overnight run will be
interrupted by a filament burn-out!

Yours sincerely,
Joergen.
J. B. Bilde-Soerensen
Materials Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 35 11 73




From: kna101-at-utdallas.edu
Date: Fri, 17 May 1996 08:56:39 -0500 (CDT)
Subject: Biotinylated HABP

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Hi,

I hope I'm sending this to the right place. This is the first time I
have tried to post, and not just reply.

I need some help tracking down a commercial supplier of biotinylated
hyaluronic acid binding protien and/or some comments on it's
specificity. I am looking for an alternative method of detecting
hyaluronic acid in tissue other than enzyme digestion. I have seen HABP
used for this, but I read in one article that there is some cross over
with chondroitin sulfate, just as there is with most enzymes. Is this
true, or is there more than one version of HABP out there?

Thanks in advance for your help.
Karen P.




From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Fri, 17 May 1996 7:16:35 -0400
Subject: Re: DAB intensification

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Message-ID: {374E9C3101F70300-at-mhs.unc.edu}
In-Reply-To: {004E9C3101F70300}

You might want to try DAB Enhancer from Innovex. It works great Doesn't
contain heavy metels and is easy to use. Simply apply for 5 minutes
after the DAB step and counter stain. I don't know who the UK supplier
is but the US fax # is 510-222-7803.
regards,
bob

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: zaluzec-at-sparc5.microscopy.com (Nestor J. Zaluzec)
Date: Fri, 17 May 1996 07:35:06 -0500
Subject: Ergonomic Keyboards

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Message-Id: {199605171231.HAA01603-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hmmm..... This isn't strictly Microscopy, but it is related
to using the computer for imaging which is part of our
charter, so I guess it's okay........ ;-)

The Apple one is a disaster. Don't buy it. The one I bought
(their first & I don't know if there is a second version)
has the numeric keypad disconnected. As a result of this
disconnection the entire keyboard "system" now has an effective
length of nearly1.5 times normal.

This means that if you use the keyboard, keypad and mouse
all the time (which I do) then you hand is constantly lifting
off the main keyboard and streching to reach the mouse, which is
now wayyyyyyyy off to one side. I tried it for a day and then
returned it. I started to get a sore shoulder and arm in addition
to numb hands.I just use a keyboard at the correct height with a wrist rest.


The only good side is that with my two numb hands I don't paint the
walls or rake the grass anymore!

Nestor
Your Friendly Neighborhood SysOp......






From: suecheng-at-codon.nih.gov (Susan Cheng)
Date: Fri, 17 May 1996 11:09:22 -0400
Subject: TEM screens

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While we are on the subject of TEM screens, has anyone noticed a change in
the quality of the screens that are being coated in the recent years? I
have always wondered whether the industry has changed their production
method or my eyes have just gradually deteriorated over the years.

I have been sending my old screens out for recoating periodically. I have
not been totally happy with the screens. Another possibility is that my
microscope may be the culprit that it may be contaminating the screen.
(Although, recently, I was not happy even with the brand new screens which
were just put in the microscope without having too much time to be
contaminated.)
Susan J.-H. Tao Cheng
NIH, Bldg 36, Rm 2A-21
Bethesda, MD 20892
Tel: (301) 496 0579
Fax: (301) 402 6875





From: Joergen Bilde-Soerensen 5709 :      j.bilde-at-risoe.dk
Date: Fri, 17 May 1996 14:26:14 +0200
Subject: SEM User's Questions

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Mary Anton wrote:

} I was told that LaB6 doesn't buy much of a brightness improvement
} over W and therefore isn't worth the added expense, is this your
} experience?

Hi Mary,
I fully agree with John Best's remark that "technical advantages of
LaB6 aside, the extended life time is worth it". This becomes
particularly important if you are going to use automated procedures
running overnight. We upgraded our JEOL 840 microscope from W to
LaB6 filaments because we are often running automated overnight
measurements of electron back scattering patterns. With an average life
time of 50 hrs for a W filament, there is a probability of 1/3 that an
overnight run will be interrupted by a filament burn-out !

Yours sincerely,
Joergen
------------------------------------------------------------------
J. B. Bilde-Soerensen
Materials Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 35 11 73




From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Fri, 17 May 1996 10:52:56 -0500 (EST)
Subject: Re: Service contracts

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Dear Greg,

Fight this "service contract" tooth and nail, at least for your electron
microscopes. These types of services work well for normal routine stuff, but
not at all for major repairs. Like any "insurance" they will try and get out
of paying for repairs. An example, our SEM developed a vacuum leak, we called
our service contract provided, within a day they were here and "fixed it". A
week later another vacuum leak occured, this time they came with a mass spec
detector and really found the leak. This second time, all day was spent in
the lab fixing this "minor" vacuum leak. How would this company your
institution is planning on dealing with handle this??

Anyway, fight this!
Best of Luck,
Ed Calomeni
Medical College of Ohio
Toledo, OH 43699
emlab-at-opus.mco.edu




From: MCCLELLAN DAVID S :      MCCLELLAN_DAVID_S-at-lilly.com
Date: Fri, 17 May 1996 14:11:53 +0000 (GMT)
Subject: EDS- Evaluating EDS systems for SEM - Need help

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Message-Id: {9605171929.AA24887-at-crdems.ge.com}


I am attempting to evaluate EDS systems for use on an SEM. Has anyone done
such an evaluation, and is there documentation you could e-mail me? My samples
are typically pharmaceutical powders, packaging material, filter residues, etc.
The SEM I use is actually an ElectroScan ESEM, model E-3.

Please reply directly to my e-mail address:

DM-at-lilly.com

Thank you!

David





From: Glen Prusky :      pruskyg-at-HG.ULETH.CA
Date: Fri, 17 May 1996 14:57:16 -0600
Subject: Myelin Stain

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Message-ID: {319CE82C.239E-at-hg.uleth.ca}

Microscopists,
Does anyone have a good protocol for a Sudan Black or other myelin stain
that can be used on brain sections? Thanks a bundle. Glen
--
Glen T. Prusky Ph.D., Department of Psychology, The University of
Lethbridge, 4401 University Drive, Lethbridge, AB, Canada T1K 3M4
pruskyg-at-hg.uleth.ca http://www.uleth.ca/psy/index.htm
Office-403-329-5161 Lab-403-329-2410 Fax-403-329-2555




From: David.Rothbard-at-ipst.edu (David Rothbard)
Date: Fri, 17 May 1996 08:49:50 -0500
Subject: Re: TEM cooling system additives

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} I am curious as to what other labs use in their TEM closed circuit cooling
} systems as an anti-algae, anti-corrosion agent. Are these treatments
} successful and how often do they need replacing?

Allan

Once a system is clean, we use about 5-8% ethylene glycol (cheapest=
laboratory grade) in distilled water. In systems we run constantly we can=
change it every two years if it looks clean. A very slight green tinge=
after that time suggests there has been some copper reaction, but it=
doesn't worry us. In systems that are not run for a few months, we see=
some cloudiness develop.

--
David Rothbard
Institute of Paper Science and Technology






From: jfb-at-uidaho.edu (franklin bailey)
Date: Fri, 17 May 1996 09:30:00 -0700 (PDT)
Subject: June PNEMS meeting

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The Pacific Northwest Electron Microscopy Society is planning a workshop
entitled "Current Techniques in Microtomy of Materials and Life Sciences"
presented by Michele Wilhite of RMC. The workshop will be held at the
University of Idaho in Moscow, ID on Saturday, June 22, and will include
ultramicrotomy of polymers at room temperature and cryo tempertures. Also
on the agenda will be cryo-ultramicrotomy and immunolabeling of biological
samples, as well as surface preparation for optimum edx and backscatter
analysis.

On the evening of the 21st, a dinner buffet and business meeting is planned.

If you are interested in attending either/or, please RSVP by 21 May to
jfb-at-uidaho.edu, or fax to (208)885-8937.

Thanks and I hope to see you here.

Franklin Bailey





From: suecheng-at-codon.nih.gov (Susan Cheng)
Date: Fri, 17 May 1996 16:59:00 -0400
Subject: TEM screen

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While we are on the subject of TEM screens, has anyone noticed a change in
the quality of the screens that are being coated in the recent years? I
have always wondered whether the industry has changed their production
method or my eyes have just gradually deteriorated over the years.

I have been sending my old screens out for recoating periodically. I have
not been totally happy with the screens. Another possibility is that my
microscope may be the culprit that it may be contaminating the screen.
(Although, recently, I was not happy even with the brand new screens which
were just put in the microscope without having too much time to be
contaminated.)

Susan J.-H. Tao Cheng
NIH, Bldg 36, Rm 2A-21
Bethesda, MD 20892
Tel: (301) 496 0579
Fax: (301) 402 6875





From: rms-at-vax.ox.ac.uk (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Fri, 17 May 1996 07:48:24 -0500
Subject: Subscribing

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Subscribe Microscopy rms-at-vax.ox.ac.uk


The Royal Microscopical Society would like to subscribe to the Microscopy
Listserver, and would like to post the following message.



MICRO 96 - International Microscopy Conference and Exhibition

Main theme: Probes in Light, Electron and Digital Microscopy

2-4 July 1996

Novotel, Hammersmith, London W6


Conference topics include:

Green fluorescent proteins
Confocal microscopy and 3D imaging
Scanning probe microscopy
Fluorescent, fluorogenic and luminescent probes
Flow cytometry
NO synthase
Immunogold probes for LM and EM
Catalysis
Particles and pollution
Low energy filtering microscopy
Electron backscattering
SEM development
Biomaterials
Paper


Exhibition

There will be an extensive exhibition by 75 companies. For free tickets, a list
of exhibitors, stand plan and an exhibition preview please contact the Royal
Microscopical Society.

For further details on the MICRO 96 Conference and/or Exhibition or any other
RMS activities please contact:

The Royal Microscopical Society
37-38 St Clements
Oxford OX1 4AJ
UK
Tel +44 (0)1865 248768
Fax +44 (0)1865 791237
rms-at-vax.ox.ac.uk


Thank you.
Best wishes
Sue Betteridge










From: rjpalmer-at-utkux1.utk.edu (Robert J. Palmer Jr.)
Date: Fri, 17 May 1996 11:05:25 -0400
Subject: Subscribing

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Message-Id: {9605171458.AA15463-at-utkux.utcc.utk.edu}

What about the wonderful sounding "tru-form w/pointer" from Adesso? This
thing has a two-button touch-pad built into the board at the juncture of
the two key pads and it can be used "without removing your hands from the
keyboard". I'm really thinking about buying this for exactly the reasons
stated below!

} Hmmm..... This isn't strictly Microscopy, but it is related
} to using the computer for imaging which is part of our
} charter, so I guess it's okay........ ;-)
}
} The Apple one is a disaster. Don't buy it. The one I bought
} (their first & I don't know if there is a second version)
} has the numeric keypad disconnected. As a result of this
} disconnection the entire keyboard "system" now has an effective
} length of nearly1.5 times normal.
}
} This means that if you use the keyboard, keypad and mouse
} all the time (which I do) then you hand is constantly lifting
} off the main keyboard and streching to reach the mouse, which is
} now wayyyyyyyy off to one side. I tried it for a day and then
} returned it. I started to get a sore shoulder and arm in addition
} to numb hands.I just use a keyboard at the correct height with a wrist rest.
}
}
} The only good side is that with my two numb hands I don't paint the
} walls or rake the grass anymore!
}
} Nestor
} Your Friendly Neighborhood SysOp......
microscopy-at-sparc5.microscopy.commicroscopy-at-sparc5.microscopy.com





From: shaf :      mshaf-at-darkwing.uoregon.edu
Date: Fri, 17 May 1996 11:13:20 -0700
Subject: poor man's cold stage

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Message-Id: {2.2.32.19960517181320.006b5044-at-darkwing.uoregon.edu}
X-Sender: mshaf-at-darkwing.uoregon.edu
X-Mailer: Windows Eudora Pro Version 2.2 (32)
Mime-Version: 1.0
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We have an interest to dampen the effects seen due to the electron beam
locally heating a silicate (rock) thinsection. The user wants to cool the
specimen prior to chamber access, whereas I'm afraid of condensation
affecting my ion pumped LaB6 gun. Can anyone alleviate my fears ... or has
anyone a remedy??

TIA & cheers, shaf
{\/} /\ {\/} /\ {\/} /\ {\/} cognito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/}
Michael Shaffer, R.A. - University of Oregon Electron Probe Facility
mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu
http://darkwing.uoregon.edu/~mshaf/epmahome/





From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 5/16/96 1:39 PM
Subject: Used equipment suppliers

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I am looking for sources of used equipment such as equipment brokers etc.
Specifically the types of equipment include coating, microscopy and
mechanical test equipment. Secondly I'm also trying to locate sources of
discounted consumable supplies for metallurgical polishing work.

I would deeply appreciate any leads.






From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Fri, 17 May 1996 14:21:19 -0500
Subject: Re: TEM cooling system additives

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We have a dual Haskris water chiller, closed recirculating system, that cools
our two EM's. Approximately quarterly, I sprinkle a fungicide powder called
dichlorophene on top of the water in the reservoir tank in the cooling unit. It
floats on top but slowwly disolves over time. We have no problems with stuff
growing in the water. We very rarely change the water completely, just add water
to the tank once and awhile.

I last purchased dichlorophene powder in 1985 from: K. & K. Labs, 121 Express
St., Engineers Hill, Plainview, NY 11803

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 17 May 1996 11:21:56 -0400 (EDT)
Subject: Re: TEM cooling system additives

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Dear Allan,
}
} We have been recommended to use a product called "Thermoclean DC" as an
} anti-algae, anti-corrosion agent in the water of our TEM closed circuit
} cooling system. However the data supplied with the product recommends that
} the water in the system be replaced every 6-8 months. I personally do not
} feel like changing the total 240 litres of water in our two systems this
} frequently.
} I am curious as to what other labs use in their TEM closed circuit cooling
} systems as an anti-algae, anti-corrosion agent. Are these treatments
} successful and how often do they need replacing?
}
We use a product called Aqua Treet 42, which is molybdenum-based
(Z = 42 ;-)). We get it from Aqua Laboratories, Inc., P.O. Box 645, 8
Industrial Way, Amesbury MA, USA, (508) 388-3989. We adjust the concen-
tration using a test kit obtained from the same company, then adjust the
pH with NaOH to 8.0-8.5. We have never had any problems with this since
we switched over from a silica-based product (also OK, but no longer avail-
able). The water does not have to be changed.
We also installed filters in the lines, and this has saved us a lot
of grief. These do have to be changed every few months, and we check them
monthly along with the concentration of Mo and the pH.
We float a little dichlorophene on top of the water in the Haskris
circulator to stop the bugs from growing. We get that from K & K division
of ICN.
Yours,
Bill Tivol




From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Fri, 17 May 1996 12:48:18 EST
Subject: Re: Service contracts w\ CIC Agency

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This is in response to Greg Erdos' message regarding CIC Agency.

I was "encouraged" last year by our college's administrators to put
our 2 scopes on contract w\ CIC. I am approaching the 8 month period
now since I did include our new SEM on the contract. The Veterinary
teaching hospital here, as well as some other labs are also using
this company. Here are some of our observations/experiences.

CIC Agency is a Risk Management Corporation...essentially an
insurance company. Instead of contracting with your regular vendor
for preventative maintenance, ie JEOL, Phillips, etc., you contract
with CIC. Their charge for the service is typically 20-30% below
your vendor (its 26% savings on our SEM over JEOL). They include 2
preventative maintenances per year. When you need service, you call
whoever you want (ie JEOL) and schedule emergency service. The
service engineer orders the parts, does the work as usual, and hands
you a bill for parts, labor, and whatever else they charge for. You
send the bill to CIC and they pay it. The only stipulation is that
you get approval from them if the bill is expected to be over $5000.

Some of the folks here who are using them for hospital equipment are
not too pleased and are talking about switching back. I haven't
decided yet, but am sure that I won't be putting my TEM with them
this upcoming year.

I would be happy to expound on this a bit more with anyone who is
interested.





W. L. Steffens, Ph.D
Dept. of Veterinary Pathology
College of Veterinary Medicine
University of Georgia
Athens, GA 30602
STEFFENS.B-at-CALC.VET.UGA.EDU
Voice: (706) 542-5536
FAX: (706) 542=5828




From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Fri, 17 May 1996 08:46:22 -0600
Subject: Re: Anti Fade Compounds

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Netters,
Just a note about antifades. Over the years, I have seen or helped
folks compare the relative merits of several of these (DABCO, ppd, ehgo)
and the results were different each time. Because different types of
preparation were being considered (plastic sections, whole mounts and
things in between, various chromomophores) I concluded that the relative
merits of antifades are specimen/prep dependent. So, if you *really* want
the best, you may need to test your own specimen.
For the past several years, we have been using a commercial
product, "Vecta shield" from Vector labs (I have no interest in this
company or product), which has served our needs perfectly (we mostly do
plastic sections with cy-3 as a fluorochrome).
Hope this helps,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: Jeff L. Brown :      brown-at-el.wpafb.af.mil
Date: Fri, 17 May 1996 17:08:17 -0400
Subject: Ergonomic keyboards

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Message-Id: {199605171956.PAA09177-at-ns.ge.com}



I've had and continue to have problems with my wrists. Our safety officer
knew of a keyboard in another office and arranged for me to borrow it to try
out. I don't remember the brand name, but it was one that has cupped
depressions (a strange contraption when you first see it) that accommodate
the differences in finger lengths as well as allow the wrists to be in a
more natural position. I tried it out for a week. It takes a lot of
getting used to because you no longer have to reach for keys as much as on a
standard keyboard. I think had I used it for any length of time, it would
have been difficult to switch back. And since I use at least 7 different
computers at work and home, I didn't think it was worth it. One of these
days I want to try the type that just splits the keyboard near the middle
and angles the two sides. In my case, that might be the best alternative,
although I think that it would still be hard to go back and forth. Perhaps
someday the standard keyboard will be adjustable so that you can sit down at
any machine and adjust the keyboard (mouse to, maybe) to fit your own hands,
much the same way as you adjust the eyepieces on a microscope for you own eyes.

My suggestion is that you try them before you buy them. Check egonomic
equipment catalogs and not just computer supply houses.

I, too, have benefited by using something to elevate my wrist once the
orignal flareup was under control and my case seems to be mild compared to
some peoples.

Regards,




---------------------------------------------------------------------
Jeff L. Brown | Electronics Engineer
WL/AADP BLDG 620 | Heterojunction Physics Branch
2241 Avionics Circle RM C2G69 | Electron Devices Division
Wright-Patterson AFB OH 45433-7322 | Avionics Directorate
| Wright Laboratory (USAF)
=====================================================================





From: DON_STEELE-at-CCKRDC.CA.ALCAN.CA
Date: 5/16/96 11:43 PM
Subject: TEM cooling system additives

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Our variety of instruments with their variety of operators have
cooling systems maintained in a variety of ways! I don't think we've
decided which is best. We have service contracts on our major
instruments, so we rely on the contract provider to bail us out if
something goes wrong, but anyway... what we mostly use(add) is:


Dichlorophene(2,2-Methylenebis-P-chlorophenol) (Panacide). This is a
powder originally suggested by Philips for our TEM purchased circa
1979. We run a Muriatic acid descaler through every few years during
an anual maintenace visit.

or... Ethylene Glycol. This is a "conventional wisdom" solution
suggested by our air conditioning/heating people, and is what was
suggested to way back in my electron microscopy school days.
Instruments being fed this stuff also get an occaisional descaling to
clean out any corrosion, or scale from hard water. We avoid dH2O due
to it's somewhat corrossive nature.


Don Steele


______________________________ Reply Separator _________________________________


We have been recommended to use a product called "Thermoclean DC" as an
anti-algae, anti-corrosion agent in the water of our TEM closed circuit
cooling system. However the data supplied with the product recommends that
the water in the system be replaced every 6-8 months. I personally do not
feel like changing the total 240 litres of water in our two systems this
frequently.
I am curious as to what other labs use in their TEM closed circuit cooling
systems as an anti-algae, anti-corrosion agent. Are these treatments
successful and how often do they need replacing?

Thanks in advance.


Allan Mitchell
South Campus Electron Microscope Unit
School of Medical Sciences
University of Otago
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD




From: DON_STEELE-at-CCKRDC.CA.ALCAN.CA
Date: 5/14/96 8:53 AM
Subject: High-quality overheads from EM micrographs

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You can get some awfully good results from scanning an image at
anything over 100dpi and reprinting with a d2t2 (diesub) printer.

We use the Kodak XLS8600, which gives excellent results in B&W or
colour.

###############################################################
# #
# Don Steele STEELE-at-KRDC.INT.ALCAN.CA #
# ALCAN INTERNATIONAL #
# Kingston Research and Development Center #
# P. O. Box 8400 #
# Kingston, Ontario Canada K7L 5L9 #
# #
###############################################################




______________________________ Reply Separator _________________________________


Does anyone out there know how to make high-quality overheads from standard
B&W photographs (e.g., TEM images including HRTEM images)? I have always
made slides for my talks up until now. Just using the standard xerox
transparencies does not give very good results, but I have seen talks where
people use transparencies and they are remarkably good. Is there some trick
or method I am missing out on?

Roy Christoffersen
Texas Center for Superconductivity
3201 Cullen
Houston, TX 77204-5932
roy-at-bayou.uh.edu
(713) 743-8273
FAX: (713) 743-2787




From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 5/15/96 5:37 PM
Subject: SEM Users' Questions

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Hi,

I am looking into the purchase of a new conventional (W/LaB6) SEM for a
Materials Science laboratory and had a couple of questions for users of
newer SEM equipment:
1. I was told that LaB6 doesn't buy much of a brightness improvement over W
and therefore isn't worth the added expense, is this your experience?
2. With digital imaging as the fast increasing method for documentation, is
the record/film option still necessary?

All inputs are greatly appreciated as well as any other comments along these
lines. Thanks in advance.

Mary Anton
University of Connecticut
USA






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Fri, 17 May 1996 15:27:34 +1100
Subject: EM:Immonolabelling cultured fibroblasts

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One of the users of our EM Unit wishes to immunogold-label cytoskeletal
proteins in cultured fibroblasts. The cells will be lightly fixed,
processed by the "progressive lowering of temperature" technique and
embedded in Lowicryl K11M. We will use a Leica AFS substitution chamber to
process the samples. Unfortnately (for us) this person wishes to keep the
cells as a monolayer and section them "face on".
We have done this with conventional epoxy resin in the past, however to do
this in the AFS chamber may be a little difficult.
Has anybody got any suggestions on the substrates to use etc,that would be
suitable for PLT processing and embedding in Lowicryl K11M?

Many thanks,


Allan Mitchell
South Campus Electron Microscope Unit
School of Medical Sciences
University of Otago
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD



Richard Easingwood
South Campus Electron Microscope Unit
School of Medical Sciences
University of Otago
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD






From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Fri, 17 May 1996 15:43:11 +1100
Subject: TEM cooling system additives

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We have been recommended to use a product called "Thermoclean DC" as an
anti-algae, anti-corrosion agent in the water of our TEM closed circuit
cooling system. However the data supplied with the product recommends that
the water in the system be replaced every 6-8 months. I personally do not
feel like changing the total 240 litres of water in our two systems this
frequently.
I am curious as to what other labs use in their TEM closed circuit cooling
systems as an anti-algae, anti-corrosion agent. Are these treatments
successful and how often do they need replacing?

Thanks in advance.


Allan Mitchell
South Campus Electron Microscope Unit
School of Medical Sciences
University of Otago
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254

SOUTHERNMOST E.M UNIT IN THE WORLD









From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Fri, 17 May 1996 11:24:38 +1100
Subject: Re: increase immuno gold staining

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} I'm back again! Thanks for all the previous help. I love this
} place!!
}
} Today's problem involves a way to increase gold staining. I have
} tissues freshly fixed in 2% PFA (also long term fixed in PLP) and run
} up in both LRWhite and Lowicryl K4M. Can these resins be etched to
} reveal more structure? Does the antibody and gold label get much
} below the surface anyway?
} I have increased time in primary to 24hrs. and don't get much
} staining. The P.I. on this project has great LM under fluorescence
} so we know that the antibody works. I am working with a kit from E.Y.
} Laboratories for the other solutions. Any and all help is deeply
} appreciated.
}
} Linda Fox -at-wpo.it.luc.edu


GR Newman and JA Hobot: Modern acrylics for postembedding immunostaining
techniques. J Histochem Cytochem 1987; 35:971-981... talks about
penetration of reagents into LR White. They found immunoperoxidase got in,
but immunogold (they used 10-12nm) stayed on the surface. One quote "Even
storage granules within a few nanometers of the section's surface failed to
immunolabel".

Personally I hate LR White; nothing ever seems to label! Sorry I can't help
with the rest of the problem.

Diana van Driel
Dept Ophthalmology
Sydney University
AUSTRALIA 2006






From: Walter A. Mannheimer :      wamann-at-metalmat.ufrj.br
Date: Fri, 17 May 1996 14:16:37 EST3EDT
Subject: meeting in Brazil

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ANNOUNCING THE

5 th BRAZILIAN CONFERENCE ON
MICROSCOPY OF MATERIALS***MICROMAT 96***

Rio de Janeiro, Brazil October 13-16, 1996

Here is an extract from the first announcement:

Scope of the Conference: MICROMAT 96 follows four previous
meetings on Microscopy of Materials [S.Paulo (1988, 1990), Rio de
Janeiro (1992), S.Carlos (1994)] sponsored by the Brazilian Society
for Electron Microscopy, and will be held at The Marina Palace Hotel
in Rio de Janeiro from October 13 to 16, 1996.

Scientific Program:
- Electron Microanalysis and Diffraction Techniques
- Instrumentation
- New Microscopies and Techniques
- Application to Materials Research in the Academic and Industrial
Context

(Invited lecturers, oral presentations and poster sessions)

Invited Speakers (preliminary):
- M.Audier (Grenoble); U.Dahmen (Berkeley); D.Joy (Knoxville);
E.L.Hall (Schenectady); G.L'Esperance (Montreal); M.McCartney
(Tempe); K.Merkle (Argonne); Padilha (S.Paulo); M.Ruehle (Stuttgart);
P.Schabez (Mexico); A.Schwartzman (Providence); J.Vander Vort
(Reading); M.Yacaman (Mexico).

Languages: the official languages will be English and Portuguese. No
simultaneous translation will be provided.

Conference Proceedings: all communications will be published in a
special volume of Acta Microscopica.

Exhibition; Located in the poster zone, a limited number of stands
will be available for microscope manufacturers and representatives of
microscopy related techniques. Commercial exhibitors should contact
conference secretariat for stand reservation and advertisement in the
proceedings book.

Conference site and accommodation: The conference hotel is located by
the beach with a splendid view of Ipanema and Leblon. October average
temperature 25-30 C. A limited number of double occupancy rooms (US$
40.00 per person) available at the Marina Palace Hotel, other hotels,
restaurants within walking distance.

Registration fees: until July 20 th. - BSEM members professional $5o,
student $25; non members $90, $50. After July 20, $60, $35, $100,
$60.

Post Conference course: minicourse on Digital Microscopy
(Cosponsored by BSEM and Gatan Inc.(limited participants!) on
Oct.17 and 18, 1996 at The Catholic University of Rio de Janeiro
(near the Marina Palace Hotel).

Second circular for those who contact the secretariat will be sent
May/June with guidelines for submission of papers and other info.

Chairman:Guillermo Solarzano (PUC-Rio); General Coordinator Luiz
Henrique de Almeida (UFRJ) Sponsors: FINEP, CNPQ, FAPERJ, FAPESP,
FAPEMIG, FAPERS (all government non-profit agencies)

Contact Secretariat

MICROMAT 96 POBox 38090
22452-970 Rio de Janeiro Brazil
Fax + 55 21 5112182 Email sbme-at-rdc.puc-rio.br

Prof.Walter A.Mannheimer
Metallurgy and Materials Engineering
Federal University of Rio de Janeiro
POBox 68505 21945 Rio de Janeiro, Brazil
Voice 5521 280-7443 (Dept.office) 5521 590-0579 (direct)
Fax 5521 290-6626 Email: wamann-at-metalmat.ufrj.br




From: MicroToday-at-aol.com
Date: Fri, 17 May 1996 20:49:31 -0400
Subject: EDS System Selection

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Group -
David McClellan recently asked for advice on the subject of selection of
EDS systems. Due to my rather unique position relating to this thread, I
would like to address my response to the full group. As it happens, prior to
my current life, I enjoyed reasonably responsibe positions with two of the
several major EDS suppliers for some 10 plus years.
First, the EDS business is very "mature". When it comes to (pure) EDS
hardware and software from the leading 4/5 manufacturers, there is very
little difference. Excluded from this generalization is the topic of
detectors. Sure, one system might have a very minor "plus" or an equally
minor "minus". I expect that a number of readers will not agree with me -
most of which, I expect, have become "swift" with one system and happen to
find others not so "user friendly" or, otherwise, lacking..
Bottom line, as to the selection of an EDS supplier, I would recommed
the following:
1) Find users in YOUR AREA and ask for comments regarding after-sales
support. I would not put a great deal of weight on a list that might be
supplied by the manufacturer, but rather look for your own inputs. This, I
submit, today is the key to your selection!
2) If you have selected your EM manufacturer, you might talk to their
applications folks. In doing so, you should understand that they could have
their own preferences (based upon their own system knowledge) and that they
are conditioned not to recommend one supplier over another. But - if you
push, you might learn a bit.
David, GOOD LUCK!
Don Grimes, Microscopy Today





From: bozzola-at-siu.edu (John. J. Bozzola)
Date: Fri, 17 May 1996 15:17:41 -0600
Subject: CSMS Meeting Date Change

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IMPORTANT NOTE:

The Central States Microscopy Society meeting date has been changed to June
28, 1996 due to a conflict occurring during the previous week. Seems that
the Street Machines National will be meeting nearby and ALL rooms for a
radius of 60 miles have been booked already. Please amend your schedules. I
apologize for this and thank Mort Harloe who, being the early-bird that he
is, found out about the lack of rooms.

See you in Carbondale on JUNE 28th.



******************************************************************************

ENJOY MICROSCOPY AND THE GREAT OUTDOORS - WHAT A COMBINATION!

******************************************************************************

The Spring meeting of the Central States Microscopy Society will take place
on June 28, 1996 in Carbondale, IL at the Giant City State Park Lodge. The
theme of the meeting will be "Ride the Wave" (e.g., technological wave).
Innovative uses of new and established technologies (microwave, LM, SEM,
TEM, scanned probes, etc) are invited. Biological and physical science
presentations are welcome.

Cash prizes for best student presentations. Typically this ranges from
$50-100, depending upon number of entrants and quality of work presented.
Abstracts must be received 3 weeks prior to meeting to be considered in the
student competition. Follow MSA abstract guidelines.

CSMS corporate sponsors are invited to participate by means of talks and
demonstrations of equipment. Contact me well in advance of the meeting,
however, if you require tables, space or special electrical setups. There
will be no charge for CSMS corporate sponsors.

Programs, lodging recommendations, directions to meeting site will be sent
several weeks in advance of the meeting or you may e-mail me for this
information.

Speakers and presentors: please contact John Bozzola for topics/timing no
later than May 28, 1996.


******************************************************************************






#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Dave King (607)757-1248 T37/257-3 Ext DEKING-at-VNET.IBM.COM
Date: 15 May 1996 17:22:06 EDT
Subject: SEM Users' Questions

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Message-Id: {199605180438.XAA04917-at-Sparc5.Microscopy.Com}

To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}
*** Reply to note of 05/15/96 16:21

LaB6 ! Yes, it's much better than W. It's about 10x brighter, and
lasts much longer. If you can afford it, and not field emission,
go for it.

I get 1500 to 2000 hours on a LaB6 tip. Most W filaments last
about 40 hours. LaB6 tips cost about $500/each, and it takes the
same time to install as W. Alignment time depends on the
instrument (ours is a Cambridge S250). W is a mess to clean up,
and LaB6 deposits mainly flake off.

. {
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: Sara Miller :      saram-at-acpub.duke.edu
Date: Fri, 17 May 1996 20:30:54 -0400 (EDT)
Subject: Re: Service contracts

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Greg, Ed,
I couldn't agree more. EM's are special beasts. The EM company's
own service is your best bet: calamities WILL HAPPEN (Murphy's law),
and your in house service won't want to shell out for a $10K part!
Besides, if they're not experienced on working on these complicated
pieces of equipment, it may take them forever to figure it out. We have
in house service on small stuff (small centrifuges, pH meters, etc.), but
all EMs are on EM company contracts.

Sara Miller, Director
Diagnostic Virology EM Lab
Cancer Center IEM/EM Shared Resource
Surgical Pathology EM Lab

On Fri, 17 May 1996, EmLab wrote:

} Date: Fri, 17 May 1996 10:52:56 -0500 (EST)
} From: EmLab {EMLAB-at-OPUS.MCO.EDU}
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: Re: Service contracts
}
} Dear Greg,
}
} Fight this "service contract" tooth and nail, at least for your electron
} microscopes. These types of services work well for normal routine stuff, but
} not at all for major repairs. Like any "insurance" they will try and get out
} of paying for repairs. An example, our SEM developed a vacuum leak, we
called
} our service contract provided, within a day they were here and "fixed it". A
} week later another vacuum leak occured, this time they came with a mass spec
} detector and really found the leak. This second time, all day was spent in
} the lab fixing this "minor" vacuum leak. How would this company your
} institution is planning on dealing with handle this??
}
} Anyway, fight this!
} Best of Luck,
} Ed Calomeni
} Medical College of Ohio
} Toledo, OH 43699
} emlab-at-opus.mco.edu
}




From: Steven Schwarz :      sschwarz-at-morgan.ucs.mun.ca
Date: Sat, 18 May 1996 11:52:16 -0230 (NDT)
Subject: unsubscribe

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unsubscribe

==============================================================================
Steve Schwarz sschwarz-at-morgan.ucs.mun.ca
Dept. of Earth Sciences
Memorial University of Newfoundland
Newfoundland
CANADA
A1B 3X5
1-709-737-8142
-737-2589 FAX
******************************************************************************




From: Emeylan-at-aol.com
Date: Sat, 18 May 1996 15:02:09 -0400
Subject: Re: LM: Stereoscope by Wild-Heerburg

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Try

LEICA Inc.
Parts Rockley 201/767-1100 or 800/248-0123

best regards, Emile Meylan





From: John Best :      jbest-at-vicon.net
Date: Sun, 19 May 1996 16:18:30 -0700
Subject: Re: EDS System Selection

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Message-Id: {9605191055.AA17336-at-super.mhv.net}
To: nestor {Microscopy-at-Sparc5.Microscopy.Com}

Good day group,

I would like to partially agree with Don Grimes recommendataions to David
Mclellan regarding EDS selection. I thought the marketplace was mature
about 5 yeard ago (more or less), when everyone was still using PDP-11's.

I'll suggest that PGT's digital front end and the new spin off companies
that are producing inexpensive PC based EDS systems have, are, and will
be upsetting the applecart.

First the spin-offs: I think these guys (like EVEX) are doing a great
service. The traditional EDS people have been pretty slow in switching
over the standard EDS algorithms to low cost PC based platforms. The
price of a modern EDS system is roughly half (feature for feature) of a
new system five years ago.

The thing that's a bit disturbing is that we're losing some neat tools in
the process. Most of the old big 5 (Edax, Kevex-} Fisons, Tracor-} Noran,
PGT and Link) had some optional software packages for things like
automatically characterizing features based on morphology and chemistry.
These days we try do do this using PC based image analysis software on
captured images. It's just not as productive as some of the on-the-fly
approaches that had beed developed.

I think the most interesting think in EDS these days is a DSP at the
front end. I havn't been able to follow up on this, but theoretically, a
DSP should be able to deconvolute peaks that formerly had to be rejected
because they contained information about two (or more) separate xrays.
The improved throughput might make "darn real time xray imaging" a
reality. Digital front ends might also make detector sensitivity less of
a factor for many applications. Instead of deconvoluting an Xray spectra
for elemental IDing, and so called "quantitative" analysis, the digital
front end can deconvolute each individual peak, thus producing a
truly digital spectrum. Even though DSP's have been in use in other
industries for years, I think their impact might ultimately be a huge
boon to microscopists.

It would be nice (to the end users advantage, but not necessarily the
manufacturers) if ultimately the entire DSP was embedded in a box on the
detector assembly and all data was transmitted to the PC via a
STANDARDIZED digital interface.

My reccomendation for David Mclelland would be: Buy a really low cost
unit at present, because great things are going to be happening in the
future with EDS, and you'll want to save your budget for something like a
"digital facelift" after this paradym shift has had a chance to settle.

I can't wait to read the groups thoughts on the EDS system of the future.

Regards and Best Wishes to All,
John Best -- ELMDAS Co.




From: alan.wilson-at-dsto.defence.gov.au (Alan Wilson)
Date: Mon, 20 May 1996 13:41:26 +1000
Subject: Re: TEM cooling system additives

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We have not had any additive in cooling water with success for many years.
Instead, we thoroughly de-ionize the water in the closed circuit system and
keep it in this state by passing all the water through carbon filters. If
the water needs replacing then we again run it through a large
de-ionization unit. With in around 200imp. gallons (800lt) in our system I
would not like to be changing the water too often. This system serves 2
EM's, an XPS DPump and another 3 DP's.

alan.wilson-at-dsto.defence.gov.au

Dr Alan Wilson
Senior Research Scientist
Ship Structures and Materials Division
Aeronautical and Maritime Research Laboratory
Defence Science and Technology Organization
506 Lorimer St
Fishermens Bend 3207
Victoria Australia
ph 61 3 9626 7508, fax 61 3 9626 7816 or 61 3 9626 7087






From: Ronald Johnston :      johnston-at-sun1.newport.ac.uk
Date: Mon, 20 May 1996 13:53:57 +0100 (BST)
Subject: general enquiry

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I have been browsing communications on my e-mail and have found your
address. Perhaps you can help.
My quey is for information regarding the preparation and staining of
transverse sections of heather stems ( heavily lignified ) for
examination under an optical microscope.
These samples have to be softened for sectioning (boiling?).
and stained in order to count their annual rings (?)
Any help or pointers of who to ask welcome.

Ron Johnston
University Wales College Newport
johnsto-at-newport.ac.uk




From: brannign-at-asrr.arsusda.gov (Peggy Brannigan)
Date: Mon, 20 May 1996 10:25:44 -0400
Subject: in situ hybridization

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Message-Id: {9605201214.AA07532-at-super.mhv.net}
To: nestor {Microscopy-at-sparc5.microscopy.com}

Hello fellow microscopists,

In the short month or so I've been a member of the newsgroup, I haven't
seen anything posted on in situ hybridization of nucleic acids and wonder
if anyone has information on :

a. past postings on this subject - how can I access them?

b. short courses/workshops in the US in the next six months - can anyone
recommend any?

c LR -White embedded tissue - I want to localize viral nucleic acids in
plant tissue - using thin sections from samples previously embedded and
used for immunolabeling viral proteins Does anyone have any experience,
words of wisdom etc on LR White and in situ habridization?

I deeply appreciate any input you can give me - I'm totally new to this
technique - haven't even tried it yet!

Peggy

Peggy Brannigan
Electron Microscopy
Floral and Nursery Plants Research Unit
National Arboretum

Bldg. 010A R.238
10300 Baltimore Avenue
Beltsville, MD USA20705

Phone: (301) 504-6097
Fax : (301) 504-5096
Email: brannign-at-asrr.arsusda. gov







From: Theresa A. Fassel :      fassel-at-post.its.mcw.edu
Date: Mon, 20 May 1996 09:49:24 -0500 (CDT)
Subject: unsubscribe

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Please unsubscribe for the present.

Dr. Theresa A. Fassel
Sr. Research Associate fassel-at-post.its.mcw.edu
Department of Microbiology (414)-456-8410
Medical College of Wisconsin Fax (414)-266-8522
8701 Watertown Plank Road
Milwaukee, WI 53226-0509





From: RMCBTLI-at-aol.com
Date: Mon, 20 May 1996 11:54:37 -0400
Subject: PMV Cryo Microtome parts for sale

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To the users of PMV cryo microtomes:

We have the following new printed circuit boards in stock which we are
offering for sale at very reduced prices. These parts are for the 450 model
but some of them may also be used in earlier models. As these parts are
probably no longer available from PMV, you might want to pick some of these
parts up for future use. Contact Greg Becker at RMC, Tucson, AZ (520)
889-7900 or e-mail directly at rmcbtli-at-aol.com.

PMV part # Description ( all printedcircuit boards)
14150 F 303 A
14151 F 303 B
14152 F 303 C
14153 F 303 D
14154 F 303 F
14156 F 303 G

Thank you,

Greg Becker
RMC
4400 S. Santa Rita Ave
Tucson, AZ 85714
tel. (520) 889-7900
fax:(520) 741-2200






From: Philip Koeck :      Philip.Koeck-at-csb.ki.se
Date: Mon, 20 May 1996 17:02:06 +0200
Subject: TEM - Gatan cryo holder

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Posted-Date: Mon, 20 May 1996 17:02:06 +0200
Message-Id: {31A0896E.7D0C-at-csb.ki.se}
Karolinska-at-ondine.csb.ki.se, Institute-at-ondine.csb.ki.se,
Depatment-at-ondine.csb.ki.se, of-at-ondine.csb.ki.se,
Bioscience-at-ondine.csb.ki.se, Novum-at-ondine.csb.ki.se,
S-14157-at-ondine.csb.ki.se, Huddinge-at-ondine.csb.ki.se,
Sweden-at-ondine.csb.ki.se
Organization: Karolinska Institute
X-Mailer: Mozilla 3.0b3 (Win95; I)
Mime-Version: 1.0
To: MSA mailing list {Microscopy-at-Sparc5.Microscopy.Com}

Hello everybody,

we have problems with boiling nitrogen in the workstation of a Gatan
cryo holder. Particularly large bubbles form near the specimen making
transfer very difficult. Has anybody had that problem and found a
solution.

Thanks in advance,

Philip




From: tom moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Mon, 20 May 1996 13:29:48 -0500 (CDT)
Subject: Re: TEM screen recoating

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On Thu, 16 May 1996 RNBALDUC-at-ARCRIDE.EDU.AR wrote:

} Dear microscopyst:
} Please will you e-mail me protocols or technics for rebuild my old TEM screen
} thanks in advance
} F. Balducci

I would also be interested in this information.

Thomas Moninger (moninger-at-emiris.iaf.uiowa.edu)
Central Microscopy Research Facility
85 EMRB University of Iowa
Iowa City, IA 52242 319-335-8142 FAX 319-335-8049





From: wise-at-vaxa.cis.uwosh.edu
Date: Mon, 20 May 1996 14:33:02 +0000
Subject: Re: TEM screen recoating

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To all,

I am interested in elucidating the structure of membrane stacks
that are roughly 0.5 microns in diameter and 0.2 microns tall. Does anyone
out there have any expertise they could share with me on the topic of
electron tomography? I know just enough about it to be dangerous and would
like more information.

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: wise-at-vaxa.cis.uwosh.edu
Date: Mon, 20 May 1996 17:12:28 +0000
Subject: Re: TEM screen recoating

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To all,

A couple of months ago I asked this net about flatbed scanners for
my Macintosh. Now I have money and think I have it narrowed down to a Umax
Powerlook II or a Microtek Scanmaker III (both come with a variety of
software bundles). Does anyone out there have and opinion as to which one
might give the best and longest service? Of course, I have to spend the
money by Thursday.

Thanks in advance

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: BGIAMMARA-at-magnum.mco.edu
Date: Mon, 20 May 1996 13:56:08 -0500 (EST)
Subject: DAB Intensification

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Hello Jeremy,
The OTO reaction is one of the many now classic contributions
of Professor Jacob S. Hanker when he was a young investigator at Johns
Hopkins. "Osmiophilic reagents: new cytochemical principles for light
and electron microscopy." Science Vol. 146:1039-1043, 1964.
Hanker also published with Seligman, et al. "Histochemical
demonstration of some oxidized macromolecules with thiocarbohydrazide (TCH)
or thiosemicarbazide (TSC) and osmium tetroxide." J. Histochem. Cytochem.
Vol. 13:629-639, 1965.
Hope this helps.

Regards to all,
Beverly Giammara
Center for Clinical Investigations
Medical College of Ohio
Toledo, OH 43699
419-381-4996
e-mail: Bgiammara-at-gemini.mco.edu





From: raija.sormunen-at-Oulu.fi (Raija Sormunen)
Date: Tue, 21 May 1996 07:58:59 +0300 (EET DST)
Subject: Unsubscribe

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Please unsubscribe
Raija Sormunen Ph.D.
University of Oulu,
Department of Pathology
Kajaanintie 52D
90220 Oulu
Finland

E-mail Raija.Sormunen-at-oulu.fi
tel.358-81-5375962
fax.358-81-330687







From: Jouko =?iso-8859-1?Q?M=E4ki?= :      jokamaki-at-utu.fi
Date: Tue, 21 May 1996 08:07:46 +0300
Subject: Re: TEM cooling system additives

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Hello all,

Like Alan Wilson, we too use de-ionized water in our closed circuit system
without any additives. This has been the case since 1984 when our system was
built. I have a possibility of connecting up to four microscopes and two
vacuum evaporators into the circuit, each with their own pumps.
I have a filter for each equipment but no active carbon or alike.
Everything has worked very fine. The tubing is made of plastic material and
everywhere else stainless steel (acid proof). No copper was allowed, because
water dissolves very strongly copper.

Regards,

Jouko

Jouko K. M=E4ki, Ph.D., Laboratory Manager
University of Turku, Laboratory of Electron Microscopy
Kiinamyllynkatu 10 FIN-20520 TURKU FINLAND
Tel: +358 21 333 7318 GSM: +358 40 505 2521 FAX: +358 21 333 7380





From: Richard Beanland +44 1327 356363 :      richard.beanland-at-gecm.com
Date: Mon, 20 May 1996 15:43:44 +0000 (GMT)
Subject: High quality overheads

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Disclose-Recipients: prohibited

We use Kodalith to produce photographic quality overheads. I guess other
companies manufacture similar products, designed, I believe, for lithographic
processes. It's about 1 to 1.5 stops faster than ordinary photographic paper
and processes in exactly the same way, apart from drying (like polaroid
negatives, you can put it through a dryer but have to be very careful!). With
a bit of masking using overheads you can put on micron markers, frames etc. to
make it look good. A lot cheaper & quicker than buying a scanner and high
resolution laser printer.


Richard Beanland,
GMMTL,
Caswell,
Towcester,
Northants NN12 8EQ,
UK





From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 20 May 1996 10:52:34 -0400 (EDT)
Subject: Re: poor man's cold stage

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}
} We have an interest to dampen the effects seen due to the electron beam
} locally heating a silicate (rock) thinsection. The user wants to cool the
} specimen prior to chamber access, whereas I'm afraid of condensation
} affecting my ion pumped LaB6 gun. Can anyone alleviate my fears ... or has
} anyone a remedy??
}
Dear Shaf,
I have found that low-dose imaging aleviates the effects of both
heating and charging. I use a 1.2 MV instrument, so our conditions might
not be comparable to yours, but you might wish to try using a very low beam
current and LoDose or other extremely sensitive film. As dictated by the
no-free-lunch principle, you pay for the sensitivity with bigger grain.
However, this is a quick and inexpensive thing to try. Good luck.
Yours,
Bill Tivol




From: Mary Anton :      semlab-at-mail.ims.uconn.edu
Date: Mon, 20 May 1996 10:17:20 -0400
Subject: Variable pressure vs conventional SEM

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Hi to all,

Thank you for the many helpful responses to my LaB6/digital imaging
questions. I am adding a new wrinkle to my SEM purchasing plans (for a
Materials Science service laboratory); namely, the variable pressure
SEM--which can be run in the conventional or variable pressure mode. I
anticipate the use of the variable pressure option to initially be low--less
than 20%. My problem is that I want to run a LaB6 filament in conventional
SEM mode and occasionally use the variable pressure mode ( the latter buys
me a capability I don't presently have). Would I have to change to a W
filament for the occasional variable pressure application?
I would appreciate any comments, especially regarding potential problems
with these variable pressure type SEMs. Examples of uses in the
non-biological fields for the variable pressure mode would also be appreciated.


Regards,

Mary Anton
University of Connecticut
Storrs, CT
USA





From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 21 May 1996 18:58:40 GMT+1200
Subject: EPMA: Gold/Silver Standards

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I have an increasing need to standardise my EPMA for the analysis of
natural gold grains, which are usually Au/Ag alloys in the region of
60% (w/w) to 100% Au.
The 1995-1996 NIST Catalogue lists such alloys (SRMs 481 and 482), but
they seem to be available only in sets of six compositions, for
US$384 per set.
I really need only one grain (preferably } 250 um) of either the 80% or
the 60% Au, and our organisation (particularly the EPMA facility)
doesn't have much money.
I would be grateful if someone out there could sell me one such grain.

Also, I can find only two synthetic glasses (SRMs 1872 and 1873), one
is Pb-rich, the other Ba-rich.
I had the impression that they put out some synthetic glasses
suitable for use in the analysis of the usual geological major
elements.
Am I looking in the wrong place, or the wrong catalogue?

Thanks

Ritchie Sims phone: 64 9 3737599 ext 7713
Department of Geology fax: 64 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: Brett W. Cockman :      bcockman-at-uniwa.uwa.edu.au
Date: Tue, 21 May 1996 09:24:49 +0700 (WAST)
Subject: RE: general enquiry

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Hello Ronald,
I have had some limited experience in cutting woody tissue for histological
purposes and the most reliable technique has been to simply saok the
paraffin-embedded tissue in cool water for at least 12 hours or more. This
should allow you to cut reasonable sections at say 10 - 12 microns. If you
need more sections than obtained in the first ribbon(s) then just soak the
block again.
To stain the tissue just use a standard safranin O - fast green technique or
simpler still, a toluidine blue O stain. The precise details can be seen in
any reasonable plant histotechnique book e.g. 'Plant Microtechnique" by
Donald A. Johansen (1940) "Staining procedures used by the biological stain
commission' by G. Clark (1973)
Hope this helps.

Regards,

Brett Cockman

----------------------------------------------------------------------------
Brett W. Cockman
Technologist in Charge
School of Dentistry
University of Western Australia
Voice: (619-2205834)
Fax: (619-2213829)
e-mail; bcockman-at-uniwa.uwa.edu.au




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 20 May 1996 17:41:23 -0400
Subject: RE-Glycol in chillers

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Subject: Time: 5:01 PM
OFFICE MEMO RE:Glycol in chillers Date: 5/20/96

There is a potential problem with using ethylene glycol in chillers hooked to
electron microscopes, and this could arise if some of the water from the
chiller system happened to get inside the intstrument column. I know this is
an unlikely event, but it has happened in our labs a couple of times.
Ethylene glycol is a hygroscopic material that tends to attract and hold
water. It can be difficult to get it out of the cracks and crevices inside
an instrument, and so it can give rise to problems with the level of vacuum
achieved. The algicides, particularly Chloramine-T, usually are only
sparingly soluble in water, and they are used in low concentrations.
Therefore, a solution of these materials in distilled water would seem to be
less likely to leave objectionable deposites inside the instrument in the
event of an accident..
I don't know whether or not ethylene clycol is particularly likely to
promote corrosion, and would be interested to know if anyone has any
authoritative info on this matter. I can't find any reference to it in any
of my three books on corrosion.
W. C. Bigelow (bigelow-at-umich.edu)





From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Tue, 21 May 1996 09:55:33
Subject: Re: distilled water corrodes copper

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To: microscopy-at-Sparc5.Microscopy.Com

In article alan.wilson-at-dsto.defence.gov.au (Alan Wilson) writes:
} Date: Mon, 20 May 1996 13:41:26 +1000
} From: alan.wilson-at-dsto.defence.gov.au (Alan Wilson)
} Subject: Re: TEM cooling system additives

} We have not had any additive in cooling water with success for many years.
} Instead, we thoroughly de-ionize the water in the closed circuit system and
} keep it in this state by passing all the water through carbon filters. If
} the water needs replacing then we again run it through a large
} de-ionization unit. With in around 200imp. gallons (800lt) in our system I
} would not like to be changing the water too often. This system serves 2
} EM's, an XPS DPump and another 3 DP's.

} alan.wilson-at-dsto.defence.gov.au

Distilled (and I imagine de-ionised) water has a more corrosive effect on
copper piping than water with additive. Can't give a reference at this date
but this was in the (engineering?) literature back in 1967. So we always use
additives for fear of corroding cooling pipes inside lenses.

On the other hand we have a 10 micron water filter in line before each of our
microscopes, which catches most of the life in our cycled supply.

cheers,







From: Peggy Brannigan[SMTP:brannign-at-asrr.arsusda.gov]
Date: Mon, 20 May 1996 15:36:00 -0500
Subject: in situ hybridization

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Peggy,

I have our favorite in situ hybridization protocol on our Web site. See:

http://cellbio.utmb.edu/childs/childs.htm and link to
http://cellbio.utmb.edu/childs/cytochem.htm This will provide links to
our favorite cytochemical protocols and in situ hybridization is on the
list.

Best wishes!
Gwen Childs

******************************
Gwen V. Childs, Ph.D.
Professor and Vice-Chair
Program Director
Cell Biology
University of Texas Medical Branch
Galveston,
TX 77555-1043
childs-at-mbian.utmb.edu
http://cellbio.utmb.edu/childs/childs.htm

----------

Hello fellow microscopists,

In the short month or so I've been a member of the newsgroup, I haven't
seen anything posted on in situ hybridization of nucleic acids and wonder
if anyone has information on :

a. past postings on this subject - how can I access them?

b. short courses/workshops in the US in the next six months - can anyone
recommend any?

c LR -White embedded tissue - I want to localize viral nucleic acids in
plant tissue - using thin sections from samples previously embedded and
used for immunolabeling viral proteins Does anyone have any experience,
words of wisdom etc on LR White and in situ habridization?

I deeply appreciate any input you can give me - I'm totally new to this
technique - haven't even tried it yet!

Peggy

Peggy Brannigan
Electron Microscopy
Floral and Nursery Plants Research Unit
National Arboretum

Bldg. 010A R.238
10300 Baltimore Avenue
Beltsville, MD USA20705

Phone: (301) 504-6097
Fax : (301) 504-5096
Email: brannign-at-asrr.arsusda. gov







From: John Best :      jbest-at-vicon.net
Date: Tue, 21 May 1996 04:01:31 -0700
Subject: Re: EDS System Selection

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Hello all,

Yes Rick, I accidentally used the term peak instead of pulse. My
dyslexia must be acting up.

You write, "The second use of "peak" and "deconvolution" is confused. It
is still not possible at the level of individual photon events in the
detector to tell whether a particular photon came from an element line or
Bremsstrahlung. This can only be done in a statistical sense looking at
the entire spectrum, as has been done for many years. A "digital front
end" has no effect on this part of EDS spectrum processing, which has
always been digital (as in manipulating numbers in a computer)."

No, I'm not confused. I meant deconvolution of a pulse. Yes, the
Bremsstrahlung are a problem in the application of DSP technology to EDS.
And finally, of course the Xray spectra is "digital" in the sense that
it is an array of numbers manipulated by a computer. But that somehow
doesn't do justice to the possibility that a digital front end
(Brehmsstralung aside) could eliminate a bunch of a costly analog signal
processing hardware by digitizing the pulse shape, not just it's maximum
amplitude. Thus the data (from which the familiar spectra is derived) is
via digital deconvolution of the pulses and not analog preprocessing of
the pulses. Perhaps instead of using the somewhat loose term "purely
digital" I should have used something like "digital spectral
representation of digitally derived data". (Double digital :) yuk yuk.)

In closing, I'm sure your aware that we're communicating on a relatively
low bandwidth medium, and semantic mistakes will sometimes occur.
There's no need to be defensive about PGT's technologies. I think what
PGT is doing is marvelous for the EM community. If you chose to respond,
please do so without malice.

Regards,

John Best.




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 20 May 1996 16:02:53 -0400
Subject: RE-Algal growth

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Subject: Time: 3:53 PM
OFFICE MEMO RE:Algal growth Date: 5/20/96

Allan Mitchell asked about controlling algal growth in closed circuit cooling
systems. This can be a very real problem for most electron microscopes.
There are three things that can be done to control it;
1. Algal growth can be greatly retarded by excluding light from all
parts of the cooling system. This involves using opaque tubing, and keeping
the water reservoir in the water chiller covered with a light-tight cover at
all times.
2. Further control can be achieved by using an algicide. The old
standby is a product known as Chloramine-T, which is the sodium salt of
N-chloro-p-toluenesulphonamide. This is available from most specialty
chemical companies (e.g. Polysciences, Sigma, Aldrich), and is used at the
level of about 1 gram per gallon of water in the cooling system (0.25
g/litre).
3. A filter should be installed on the intake to the water line to
prevent algae and other solid materials from getting into the cooling lines
of the lenses, etc. This filter must be cleaned, and preferably replaced, on
a regular basis.
( Ref: Vacuum Methods in Electron Microscopy, by W. C. Bigelow,
Portland Press, 1994, p. 216.)
I have not had any experience with the product called Thermoclean DC;
however, we have used the Chloramine-T stuff in our several systems here at
the Univ. of Michigan for a number of years with very good success. We use
distilled water to avoid scale formation in heated parts of the system (the
stuff sold in drugstores is good enough), and add more as necessary from time
to time to keep the system up to the necessary operating level. We only
replace it when it gets dirty or otherwise contaminated, or when it is lost
due to service problems.
Good luck with your system
Wil Bigelow (bigelow-at-umich.edu)






From: Software department :      software-at-oimag.win-uk.net
Date: Tue, 21 May 1996 09:58:40
Subject: Re: EDS System Selection

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Reply-To: Software department {software-at-oimag.win-uk.net}
To: rick-at-pgt.com, Microscopy-at-Sparc5.Microscopy.Com

Thank you Rick for clarifying the confusion between deconvolution of
spectral peaks and separating individual signal pulses arising from
the x-ray detector. I certainly echo the comments in your last
paragraph.

I feel the words "The first sense, in which pulse pile-up can be
untangled, is no longer in the realm of theory." are rather
overstating the case. Practical circuits to detect pulse pile-up
have been available and present in equipment for over 20 years so
this issue is hardly "in the realm of theory". The major advances
in recent years have been in improved electronic detection of low
energy x-ray events ( { 1 keV ) which reduces pile and gives better
spectral fidelity in this low energy region.

Now that high speed ADC's are available at reasonable cost, a
digital electronic implementation is considerably more convenient
and flexible than playing around with delay lines and complex
time-variant analogue circuitry. Nevertheless, all electronic
processors are faced with the same problem: the major component of
noise at the detector head is inextricably linked with the photon
signal before the signal is digitised. "Adaptive pulse shaping" has
both benefits and disadvantages depending on the intended
application but as far as I am aware, it cannot tell the electrons
in the detector and FET when and where to move.

I am also one of those commercial guys who wants everyone to buy an
EDX system... I hope for all the right reasons.


Peter Statham
Oxford Instruments Microanalysis Group



} John Best writes:
}
} {... skipping some interesting opinions ...}
}
} } I think the most interesting think in EDS these days is a DSP at the
} } front end. I havn't been able to follow up on this, but theoretically, a
} } DSP should be able to deconvolute peaks that formerly had to be rejected
} } because they contained information about two (or more) separate xrays.
} } The improved throughput might make "darn real time xray imaging" a
} } reality. Digital front ends might also make detector sensitivity less of
} } a factor for many applications. Instead of deconvoluting an Xray spectra
} } for elemental IDing, and so called "quantitative" analysis, the digital
} } front end can deconvolute each individual peak, thus producing a
} } truly digital spectrum.
}
} The word "peak" is being used for two things here. The first sense, in
} which pulse pile-up can be untangled, is no longer in the realm of theory.
} This is what PGT's adaptive pulse processing does, and has been commercially
} available since 1993. The word "peak" is being used to describe the time
} waveform emerging from the detector preamplifier or any subsequent analog
} stages before digitization; perhaps "pulse" is a more usual term.
}
} The second use of "peak" and "deconvolution" is confused. It is still not
} possible at the level of individual photon events in the detector to tell
} whether a particular photon came from an element line or Bremsstrahlung.
} This can only be done in a statistical sense looking at the entire
} spectrum, as has been done for many years. A "digital front end" has
} no effect on this part of EDS spectrum processing, which has always been
} digital (as in manipulating numbers in a computer).
}
} DISCLOSURE: I work for PGT, so I have an obvious interest in the outcome
} of this debate.
}
} Regards to all,
}
} Rick Mott
} rick-at-pgt.com
}

--
-- Software Dept.( shared email facility )
-- Oxford Instruments Microanalysis Group
-- Halifax Road, High Wycombe, Bucks HP12 3SE, UK






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Mon, 20 May 1996 23:30:17, -0500
Subject: TEM Screens: Coating quality

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-- [ From: Charles A. Garber., Ph.d * EMC.Ver #2.10P ] --

In response to:
================
While we are on the subject of TEM screens, has anyone noticed a change
in the quality of the screens that are being coated in the recent
years? I have always wondered whether the industry has changed their
production method or my eyes have just gradually deteriorated over the
years.
======================================

For year and years, the optimum phosphors used for TEM screens were Cd
based, until that is, people found out how really bad of an actor (e.g.
highly toxic) these Cd based phosphors really were. As a result, the
phosphor industry pretty much reformulated their phosphors with less
toxic alternatives, however for the TEM application, I have certainly
been led to believe that the "substitutes" are clearly not equivalent
by any sense of the word.

From what I have seen with my own eyes over the years, going back to my
own graduate school career, at least in my own experience which might
not be representative, the coating of screens was never done with the
respect deserving of such toxic compounds. I have personally been
convinced that this is something better left in the hands of people who
do this for a full time living.

SPI has offered a screen recoating service for some years. We do not
do this in house, but in an outside facility dedicated to the coating
of phosphor screens. I have been told that they have a "life time"
supply of the original Cd based phosphor. I do not permit this kind of
activity to be performed on our premises because of the safety
concerns. We have stopped offering the Cd based phosphors as part of
our business for the same liability concerns that have caused the
manufacturers to stop producing the original materials.

Another alternative to the need to periodically recoat screens is to
replace the center of the screen with a YAG single crystal scintillator
disc. While the initial purchase price might seem a bit on the high
side, the cost is probably equivalent to two or three screen
recoatings. And you do not have to put up with the down time
associated with the changing of screens. Nothing in life is forever,
of course, but the YAG single crystal disc comes close to it, and I
think it would be safe to say that it would be impossible for a
"normal" TEM beam to "burn" a hole in one of our SPI single crystal
YAG scintillator discs being used as a TEM screen. At least I have not
ever been made aware of anyone who has ever burned such a hole in a YAG
disc modified screen.

Disclaimer: SPI Supplies both recoats screens and also supplies the
YAG single crystal scintillator discs so we would clearly have an
interest in customers not coating their own screens!

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.com

####################################
WWW: http://www.2spi.com
####################################
======================================================




From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 20 May 1996 11:25:50 -0400 (EDT)
Subject: Re: TEM screen

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}
} While we are on the subject of TEM screens, has anyone noticed a change in
} the quality of the screens that are being coated in the recent years? I
} have always wondered whether the industry has changed their production
} method or my eyes have just gradually deteriorated over the years.
}
} I have been sending my old screens out for recoating periodically. I have
} not been totally happy with the screens. Another possibility is that my
} microscope may be the culprit that it may be contaminating the screen.
} (Although, recently, I was not happy even with the brand new screens which
} were just put in the microscope without having too much time to be
} contaminated.)
}
Dear Susan,
For many years we had coated our own screens using a method which
involved suspending phosphor in gelatin and letting it settle out of sus-
pension. We got very good screens when things worked properly, but often
there would be inhomogeneities, and the skill in pouring fades when not used
often. The gelatin-based screens develop a brownish cast over the course
of about a year and fade accordingly.
We recently obtained screens from Grant Scientific and Fullam which
were prepared my a method using an organic solvent. We have just installed
three of the screens from Grant--a high-brightness screen, a focussing
screen, which has a central low-brightness-high-resolution insert, and a
transmission screen for our video system. We have evaluated these screens
vs our old ones, and we find that our old screens are brighter, but the
Grant screens are very uniform and of high resolution. The focussing screen
is of exceptional quality, and the high-brightness and video screens are
pretty good, although we would prefer them to be brighter. I am going to
talk to Grant to see if future screens could be made brighter, and I have no
reason to believe that they could not. I have no idea as yet whether the
Grant screens will have longer or shorter useful lives than our old type,
and I have not had a chance to evaluate Fullam's (high-brightness) screen.
Yours,
Bill Tivol







From: list-at-jeol.se (JEOL(Skandinaviska)AB)
Date: Mon, 20 May 1996 23:43:55 +0200
Subject: RE: TEM cooling system additives(Daniel)

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} Return-Path: Microscopy-request-at-Sparc5.Microscopy.Com
} Date: Fri, 17 May 1996 10:27:19 -0500
} From: Linda Fox {lfox1-at-wpo.it.luc.edu}
} To: Microscopy-at-aaem.amc.anl.gov
} Subject: RE: TEM cooling system additives
}
} The water in our recirculating unit was recently changed under our
} yearly service contract. The engineer filled it with water from our
} Milli-Q system and had us order one liter of Ethylene Glycol (Fisher
} Scientific) to add to the system. I just checked, and there is
} nothing growing, that I can see, however there is a faint metallic
} sheen across surface of the water. Hope this helps.
} Linda M. Fox lfox1-at-wpo.it.luc.edu
}
}





From: dbd1-at-uclink4.berkeley.edu
Date: Tue, 21 May 1996 08:52:09 -0700 (PDT)
Subject: How do I unsubscribe

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Help me! I need to unsubscribe from the bulletin board and I don't know
how. Can someone send me some instructions?

psic-at-uclink4.berkeley.edu


Doug Davis
Staff Research Associate
Electron Microscope Lab
University of California
Berkeley, CA 94720
(510) 642-2085, fax 643-6207
dbd1-at-uclink4.berkeley.edu







From: davilla-at-4pi.com (Scott D. Davilla)
Date: Tue, 21 May 1996 13:40:32 -0600
Subject: Re: EDS System Selection

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Two interesting Patents on the subject of "pulse deconvolution" are;

#05276615, 1994, "Nuclear detection device especially a gamma-camera type
device, with deconvolution filters having an inverse transfer function"

#05307299, 1994, "Circuit arrangement for the digital processing of
semiconductor detector signals"

Scott




-----------------------------------------------------------------------
Scott D. Davilla Phone: 919 489-1757 (tel)
4pi Analysis, Inc. Fax: 919 489-1487 (fax)
3500 Westgate Drive, Suite 403 email: davilla-at-4pi.com
Durham, North Carolina 27707-2534 web: http://www.4pi.com







From: ebs-at-ebsciences.com
Date: Tue, 21 May 1996 12:43:44 -0500
Subject: re: LaB6 vs tungsten

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At 05:18 PM 5/16/96 -0600, you wrote:
"The 10x brightness which has been touted for years only occurs for smaller
cone angles (60 degrees)."

LaB6 cathodes with 60 degree cone angles are readily available for all TEMs
and SEMs, although they are more commonly used with TEMs.

I would be happy to supply technical data sheets on LaB6 brightness to
anyone who is interested.

Disclaimer: Energy Beam Sciences manufactures tungsten filaments and
distributes Denka LaB6 cathodes.

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: list-at-jeol.se (JEOL(Skandinaviska)AB)
Date: Mon, 20 May 1996 23:44:25 +0200
Subject: Re: TEM cooling system additives(Daniel)

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} Return-Path: Microscopy-request-at-Sparc5.Microscopy.Com
} Date: Fri, 17 May 1996 08:49:50 -0500
} To: Microscopy-at-Sparc5.Microscopy.Com
} From: David.Rothbard-at-ipst.edu (David Rothbard)
} Subject: Re: TEM cooling system additives
}
} } I am curious as to what other labs use in their TEM closed circuit cooling
} } systems as an anti-algae, anti-corrosion agent. Are these treatments
} } successful and how often do they need replacing?
}
} Allan
}
} Once a system is clean, we use about 5-8% ethylene glycol (cheapest
laboratory grade) in distilled water. In systems we run constantly we can
change it every two years if it looks clean. A very slight green tinge
after that time suggests there has been some copper reaction, but it doesn't
worry us. In systems that are not run for a few months, we see some
cloudiness develop.
}
} --
} David Rothbard
} Institute of Paper Science and Technology
}
}
}





From: alan.wilson-at-dsto.defence.gov.au (Alan Wilson)
Date: Tue, 21 May 1996 08:49:15 +1000
Subject: Re: Re: TEM cooling system additives

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X-Sender: alan.wilson-at-SoMPop.dsto.defence.gov.au
Message-Id: {v01540b00adc6a6852fea-at-[146.221.36.219]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} I would have thought that totally de-ionized water would cause a
} problem, since it is "seeking ions", which can lead to
} corrosion. This is the same reason that you never want to
} put deionized water in your car's radiator. Perhaps
} you mean "distilled water"?

No, the water is initially deionized through a resin exchange system. One
point to note is that all of the plumbing is copper with some brass
fittings. (and stainless steel in the microscopes). This system has run
for } 10 years with no problems.
One of our "plumbers" put a galv. fitting in the system which was noticed
after a couple of days. The fittings was severely attacked in this short
time. Thus a caveat may be required - use deionized water only if the
plumbing is single metal, or copper/brass.

alan.wilson-at-dsto.defence.gov.au

Dr Alan Wilson
Senior Research Scientist
Ship Structures and Materials Division
Aeronautical and Maritime Research Laboratory
Defence Science and Technology Organization
506 Lorimer St
Fishermens Bend 3207
Victoria Australia
ph 61 3 9626 7508, fax 61 3 9626 7816 or 61 3 9626 7087






From: GANTZ-at-med-biophd.bu.edu
Date: Tue, 21 May 1996 15:02:01 -0400 (EDT)
Subject: Gatan Cryo Workstation

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Dear Philip:
To help alleviate the bubbling disturbances in the workstation
during specimen transfer, we have found that the insertion of a piece
of filter paper approximately 2cm x 4cm in a vertical position over the
hole where the holder enters the workstation is worthwhile. To reduce
the chance of the grid bubbling out of the holder after transfer and
before replacement of the hexring, use a liquid nitrogen level just above
the holder tip. If you are fast, the split second or so that the grid is
in the cold band above the liquid surface will not affect ice quality.
Alternatively, try a heavier grid such as molybdenum; it won't
bounce around as much.
Good luck. We can discuss further through direct E-Mail.

Donald Gantz
Boston Univ Med Schoo
Gantz-at-Med-biophd.bu.edu




From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 21 May 1996 10:16:19 -0500 (EDT)
Subject: Re: TEM Screens: Coating quality

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}
} -- [ From: Charles A. Garber., Ph.d * EMC.Ver #2.10P ] --
[skip]
} Nothing in life is forever,
} of course, but the YAG single crystal disc comes close to it, and I
} think it would be safe to say that it would be impossible for a
} "normal" TEM beam to "burn" a hole in one of our SPI single crystal
} YAG scintillator discs being used as a TEM screen. At least I have not
} ever been made aware of anyone who has ever burned such a hole in a YAG
} disc modified screen.

Dear Chuck,
Is this also true for those operating in diffraction mode? I have
heard of YAG developing a permanent set of dark spots, which would be most
distracting.
Yours,
Bill Tivol




From: list-at-jeol.se (JEOL(Skandinaviska)AB)
Date: Mon, 20 May 1996 23:59:08 +0200
Subject: Re: Service contracts (Lauri)

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} Return-Path: Microscopy-request-at-Sparc5.Microscopy.Com
} Date: Fri, 17 May 1996 10:52:56 -0500 (EST)
} From: EmLab {EMLAB-at-OPUS.MCO.EDU}
} Subject: Re: Service contracts
} To: microscopy-at-Sparc5.Microscopy.Com
} X-VMS-To: IN%"microscopy-at-msa.microscopy.com"
}
} Dear Greg,
}
} Fight this "service contract" tooth and nail, at least for your electron
} microscopes. These types of services work well for normal routine stuff, but
} not at all for major repairs. Like any "insurance" they will try and get out
} of paying for repairs. An example, our SEM developed a vacuum leak, we called
} our service contract provided, within a day they were here and "fixed it". A
} week later another vacuum leak occured, this time they came with a mass spec
} detector and really found the leak. This second time, all day was spent in
} the lab fixing this "minor" vacuum leak. How would this company your
} institution is planning on dealing with handle this??
}
} Anyway, fight this!
} Best of Luck,
} Ed Calomeni
} Medical College of Ohio
} Toledo, OH 43699
} emlab-at-opus.mco.edu
}
}





From: Dave.Strecker-at-po.cle.ab.com (Dave Strecker)
Date: Tue, 21 May 1996 17:39:08 -0400
Subject: subscribe

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Mime-Version: 1.0

dave.strecker-at-ab.com




From: list-at-jeol.se (JEOL(Skandinaviska)AB)
Date: Mon, 20 May 1996 23:45:59 +0200
Subject: Re: TEM cooling system additives

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} Return-Path: Microscopy-request-at-Sparc5.Microscopy.Com
} Date: Fri, 17 May 1996 14:21:19 -0500
} From: "Gib Ahlstrand" {giba-at-puccini.crl.umn.edu}
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: Re: TEM cooling system additives
}
} We have a dual Haskris water chiller, closed recirculating system, that cools
} our two EM's. Approximately quarterly, I sprinkle a fungicide powder called
} dichlorophene on top of the water in the reservoir tank in the cooling
unit. It
} floats on top but slowwly disolves over time. We have no problems with stuff
} growing in the water. We very rarely change the water completely, just add
water
} to the tank once and awhile.
}
} I last purchased dichlorophene powder in 1985 from: K. & K. Labs, 121 Express
} St., Engineers Hill, Plainview, NY 11803
}
} Gib Ahlstrand, MMS Newsletter Editor
} Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
} 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
} 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
}
} "MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996
}
}





From: RMCBTLI-at-aol.com
Date: Tue, 21 May 1996 18:49:20 -0400
Subject: MacElwain Tissue Chopper: Request for Info

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A colleague of mine is looking for a tissue chopper (NOT a Vibratome). Does
anyone have any info that might help her?

I've heard that the tissue chopper might be back in production after several
years' absence, but I haven't been able to get any details. Maybe this is
just a rumor??

I would appreciate any information you might be able to provide. Thanks in
advance.

Bob Chiovetti
Applications Lab Manager
RMC
1-800-453-2242
Fax 520-741-2200
RMCBTLI-at-aol.com




From: list-at-jeol.se (JEOL(Skandinaviska)AB)
Date: Mon, 20 May 1996 23:46:26 +0200
Subject: Re: TEM cooling system additives

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} Return-Path: Microscopy-request-at-Sparc5.Microscopy.Com
} X-Sender: alan.wilson-at-SoMPop.dsto.defence.gov.au
} Date: Mon, 20 May 1996 13:41:26 +1000
} To: Microscopy-at-Sparc5.Microscopy.Com
} From: alan.wilson-at-dsto.defence.gov.au (Alan Wilson)
} Subject: Re: TEM cooling system additives
}
} We have not had any additive in cooling water with success for many years.
} Instead, we thoroughly de-ionize the water in the closed circuit system and
} keep it in this state by passing all the water through carbon filters. If
} the water needs replacing then we again run it through a large
} de-ionization unit. With in around 200imp. gallons (800lt) in our system I
} would not like to be changing the water too often. This system serves 2
} EM's, an XPS DPump and another 3 DP's.
}
} alan.wilson-at-dsto.defence.gov.au
}
} Dr Alan Wilson
} Senior Research Scientist
} Ship Structures and Materials Division
} Aeronautical and Maritime Research Laboratory
} Defence Science and Technology Organization
} 506 Lorimer St
} Fishermens Bend 3207
} Victoria Australia
} ph 61 3 9626 7508, fax 61 3 9626 7816 or 61 3 9626 7087
}
}
}





From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Tue, 21 May 1996 13:45:30 -0400 (EDT)
Subject: Re: TEM screen recoating

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I used to have 2 AEI 801 electron microscopes and always recoated my screens.
It can be frustrating at times but I got real nice screens. I used the
phoshor from JEOL and I got good contrast and brightness on the screen.
I tried other phosphors from different companies but the JEOL phosphor
seemed to work the best. If anyone is interested, I can fax a copy of
how I recoated the screen. I will e-mail if you'd rather. No I am not
connected with JEOL. I do have 2 JEOL scopes and 1 Zeiss scope.

Peace,

Phil Rutledge, Director
Center for Microscopy




From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 21 May 1996 11:56:20 -0500
Subject: EM chil-Glycol

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Message-ID: {n1379442548.53359-at-msmail.tmc.tulane.edu}

1) Every instrument I used since 1979 (Joel, Zeiss, Hitachi) contained glycol
in the water and have yet to detect problems outlined in this discussio:

a) Make sure that you contact the chiller manufacturer to get proper ratio of
water to glycol,
b) Yes, glycol COULD harden seals with leak potential. I think that regular
maintenance and replacement of old glycol can help.
c) Mix with the glycol only dianized or distilled water, never tap water!

*********************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} ----} Prof. Pathology & Otolaryngology *
*http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html*
*********************************************************************




From: list-at-jeol.se (JEOL(Skandinaviska)AB)
Date: Mon, 20 May 1996 23:59:51 +0200
Subject: Service contracts (Lauri)

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} Return-Path: Microscopy-request-at-Sparc5.Microscopy.Com
} Date: Thu, 16 May 1996 11:23:55 GMT
} X-Sender: gwe-at-biotech.ufl.edu
} To: Microscopy-at-Sparc5.Microscopy.Com
} From: gwe-at-biotech.ufl.edu (Greg Erdos)
} Subject: Service contracts
}
} The University of Florida is strongly urging all of us to drop our
} service contracts on all types of equipment in favor of going with a company
} called CIC Agency who will handle service payments. We chose the service
} provider and they pay for the services charges. It sort of like an HMO. We
} pay a fixed annual fee.
}
} Has anyone had experience with CIC or similar? If so could you share with
} us your feelings.
} *******************************************************
} Greg Erdos Phone: 352-392-1295
} Scientific Director,
} ICBR Electron Microscopy Core Lab
} 218 Carr Hall Fax: 352-846-0251
} University of Florida E-mail: gwe-at-biotech.ufl.edu
} Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/
}
} *******************************************************
}
}





From: list-at-jeol.se (JEOL(Skandinaviska)AB)
Date: Mon, 20 May 1996 23:46:13 +0200
Subject: RE: TEM cooling system additives

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} Return-Path: Microscopy-request-at-Sparc5.Microscopy.Com
} Alternate-Recipient: allowed
} Auto-Forwarded: prohibited
} Content-Return: allowed
} Disclose-Recipients: prohibited
} Conversion: allowed
} Importance: normal
} Priority: normal
} Sensitivity: Company-Confidential
} Subject: RE: TEM cooling system additives
} From: "Scott D. Walck WL/MLBT" {walcksd-at-ml.wpafb.af.mil}
} To: Linda Fox {lfox1-at-wpo.it.luc.edu} ,
} Microscopy ListServer {Microscopy-at-Sparc5.Microscopy.Com}
} Date: Fri, 17 May 96 20:30:21 -0400
}
} } The water in our recirculating unit was recently changed under our
} } yearly service contract. The engineer filled it with water from our
} } Milli-Q system and had us order one liter of Ethylene Glycol (Fisher
} } Scientific) to add to the system. I just checked, and there is
} } nothing growing, that I can see, however there is a faint metallic
} } sheen across surface of the water. Hope this helps.
} } Linda M. Fox lfox1-at-wpo.it.luc.edu
} }
} You might want to check this out, but I once was told by a reliable source
(professor that taught and worked in corrosion) that ethylene glycol by
itself is corrosive and that in antifreeze there are antioxidants and such
added to it to prevent corrosion. Now I might be suffering from old age and
this was given to me a long time ago, but I'm pretty sure that I got this
right. If anyone out there in microland knows for sure, I would like to
know if what I'm relaying here is in fact true.
}
} - -Scott Walck
}
}





From: sys4rsch-at-travel-net.com (Mark Priebe)
Date: Tue, 21 May 1996 22:26:42 -0400
Subject: Biomaterials Congress Exhibit Passes

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We have complimentary 1 day exhibit passes available for the 5th
World Biomaterials Congress held in Toronto May28th -June 2nd in Toronto.

We just got a hold of these tickets & I'd like to notify individuals who
were planning on attending to see Digital Instrument's SPM at the Congress.
These passes will probably save you about $75.

If this applies to you, please contact Art Priebe at 613-832-0094 or E-mail us
at sys4rsch-at-travel-net.com.

(We are the Canadian Representatives for D.I.)


Thankyou

Mark Priebe






Mark Priebe
Sales Representative
Systems for Research Corp
(sys4rsch-at-travel-net.com)





From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Mon, 20 May 1996 11:51:31 -0400 (EDT)
Subject: RE: Staining lignified stem tissue/general enquiry

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X-NUPop-Charset: English

In message Mon, 20 May 1996 13:53:57 +0100 (BST),
Ronald Johnston {johnston-at-sun1.newport.ac.uk} writes:

} I have been browsing communications on my e-mail and have found your
} address. Perhaps you can help.
} My quey is for information regarding the preparation and staining of
} transverse sections of heather stems ( heavily lignified ) for
} examination under an optical microscope.
} These samples have to be softened for sectioning (boiling?).
} and stained in order to count their annual rings (?)
} Any help or pointers of who to ask welcome.
}
} Ron Johnston
} University Wales College Newport
} johnsto-at-newport.ac.uk
}
-----------
If you are basically interested in counting the annual ring, a lignin stain
such as Phlolroglucin should give you very quick results. Place the sections in
0.1 gram of phloroglucin in 10 cc of 95% ETOH for a few minutes and transfer
to 25% HCL. Lignified tissue will appear red-violet. If your material would
lend itself to it, you may try even a faster method by staining the cut end
of the wood, provided the cut is smooth. You could "paint" the cut end
with phloroglucin and HCL and view the wood under a dissection microscope to
count the annual rings! Good Luck!

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology &
Director, Cornell Integrated Microscopy Center
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: William Tivol :      tivol-at-wadsworth.org
Date: Tue, 21 May 1996 09:59:39 -0500 (EDT)
Subject: Re: TEM - Gatan cryo holder

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Karolinska-at-ondine.csb.ki.se, Institute-at-ondine.csb.ki.se,
Depatment-at-ondine.csb.ki.se, of-at-ondine.csb.ki.se,
Bioscience-at-ondine.csb.ki.se, Novum-at-ondine.csb.ki.se, S-14157-at-o

} we have problems with boiling nitrogen in the workstation of a Gatan
} cryo holder. Particularly large bubbles form near the specimen making
} transfer very difficult. Has anybody had that problem and found a
} solution.

Dear Philip,
Once everything gets cold, there should no longer be much boiling.
If you have the heater running, or if the temp has not reached steady state,
there will be problems. I usually pour a lot of LN2 in the station and
after it reaches steady state, I pour some more LN2 in so that there is
liquid in the depressions in the aluminum piece surrounding the stage,
but the level is below that of the specimen. The secret is to be very pa-
tient about everything and not to worry if a little LN2 comes out the side
of the station (where the stage goes in). Good luck.
Yours,
Bill Tivol




From: tom moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Tue, 21 May 1996 09:08:27 -0500 (CDT)
Subject: Re: your mail

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On Mon, 20 May 1996 wise-at-vaxa.cis.uwosh.edu wrote:

} To all,
}
} A couple of months ago I asked this net about flatbed scanners for
} my Macintosh. Now I have money and think I have it narrowed down to a Umax
} Powerlook II or a Microtek Scanmaker III (both come with a variety of
} software bundles). Does anyone out there have and opinion as to which one
} might give the best and longest service? Of course, I have to spend the
} money by Thursday.
}
} Thanks in advance
}
} Bob
}
}
} Robert R. Wise, PhD
} Director, UWO Electron Microscope Facility
} Department of Biology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} (414) 424-3404 tel
} (414) 424-1101 fax
} wise-at-vaxa.cis.uwosh.edu
}

We have had a Microtek IIHR with the transparency adaptor for a few months now
and have been quite pleased. It came with full Adobe Photoshop (which we
wanted anyway) and some optical character recognition software. We had a
bit of a conflict with the 16 bit scanner running under 32 bit '95 but a
fix-it DLL from Adobe cured the problem. THe only other problem we have
is a bit of inconsistancy in image quality when scanning dense TEM negs,
and that can usually be solved by adjusting the setting manually. From
what I have heard the III is equal to or better than the IIHR. Hope this
helps.

Tom

Thomas Moninger (moninger-at-emiris.iaf.uiowa.edu)
Central Microscopy Research Facility
85 EMRB University of Iowa
Iowa City, IA 52242 319-335-8142 FAX 319-335-8049





From: list-at-jeol.se (JEOL(Skandinaviska)AB)
Date: Mon, 20 May 1996 23:45:42 +0200
Subject: Re: TEM cooling system additives

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} Return-Path: Microscopy-request-at-Sparc5.Microscopy.Com
} From: William Tivol {tivol-at-wadsworth.org}
} Subject: Re: TEM cooling system additives
} To: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
} Date: Fri, 17 May 1996 11:21:56 -0400 (EDT)
} Cc: microscopy-at-Sparc5.Microscopy.Com
} Content-Length: 1463
}
} Dear Allan,
} }
} } We have been recommended to use a product called "Thermoclean DC" as an
} } anti-algae, anti-corrosion agent in the water of our TEM closed circuit
} } cooling system. However the data supplied with the product recommends that
} } the water in the system be replaced every 6-8 months. I personally do not
} } feel like changing the total 240 litres of water in our two systems this
} } frequently.
} } I am curious as to what other labs use in their TEM closed circuit cooling
} } systems as an anti-algae, anti-corrosion agent. Are these treatments
} } successful and how often do they need replacing?
} }
} We use a product called Aqua Treet 42, which is molybdenum-based
} (Z = 42 ;-)). We get it from Aqua Laboratories, Inc., P.O. Box 645, 8
} Industrial Way, Amesbury MA, USA, (508) 388-3989. We adjust the concen-
} tration using a test kit obtained from the same company, then adjust the
} pH with NaOH to 8.0-8.5. We have never had any problems with this since
} we switched over from a silica-based product (also OK, but no longer avail-
} able). The water does not have to be changed.
} We also installed filters in the lines, and this has saved us a lot
} of grief. These do have to be changed every few months, and we check them
} monthly along with the concentration of Mo and the pH.
} We float a little dichlorophene on top of the water in the Haskris
} circulator to stop the bugs from growing. We get that from K & K division
} of ICN.
} Yours,
} Bill Tivol
}
}





From: M.V. Parthasarathy :      mvp2-at-cornell.edu
Date: Mon, 20 May 1996 12:07:27 -0400 (EDT)
Subject: RE: in situ hybridization

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X-NUPop-Charset: English

In message Mon, 20 May 1996 10:25:44 -0400,
brannign-at-asrr.arsusda.gov (Peggy Brannigan) writes:

} Hello fellow microscopists,
}
} In the short month or so I've been a member of the newsgroup, I haven't
} seen anything posted on in situ hybridization of nucleic acids and wonder
} if anyone has information on :
}
} a. past postings on this subject - how can I access them?
}
} b. short courses/workshops in the US in the next six months - can anyone
} recommend any?
}
} c LR -White embedded tissue - I want to localize viral nucleic acids in
} plant tissue - using thin sections from samples previously embedded and
} used for immunolabeling viral proteins Does anyone have any experience,
} words of wisdom etc on LR White and in situ habridization?
}
} I deeply appreciate any input you can give me - I'm totally new to this
} technique - haven't even tried it yet!
}
} Peggy
}
} Peggy Brannigan
} Electron Microscopy
} Floral and Nursery Plants Research Unit
} National Arboretum
}
} Bldg. 010A R.238
} 10300 Baltimore Avenue
} Beltsville, MD USA20705
}
} Phone: (301) 504-6097
} Fax : (301) 504-5096
} Email: brannign-at-asrr.arsusda. gov
}
--------------
Although a few have used LR White embedded tissue for in situ hybridization
studies, you will find that most prefer Lowicryls. I suggest that you refer
to the recent article by Xingxiang Li & Thomas W. Okita (1995), "Localization
of RNA by high resolution in situ hybridization" In: Methods in Plant Cell
Biology; Edts. D.W. Galbraith, H.J. Bohnert & D.P. Bourque, Vol 49, Part A,
pages 185-199; Academic Press. Good Luck!

*************************************************************************
M.V. Parthasarathy
Professor of Plant Biology &
Director, Cornell Integrated Microscopy Center
Section of Plant Biology
228 Plant Science Building
Cornell University, Ithaca, NY 14850
Telephone: (607) 255-1734
Fax: (607) 255-5407
E-mail: mvp2-at-cornell.edu
***********************************************************************




From: list-at-jeol.se (JEOL(Skandinaviska)AB)
Date: Mon, 20 May 1996 23:57:47 +0200
Subject: Re: Service contracts w\ CIC Agency (Till Lauri)

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} Return-Path: Microscopy-request-at-Sparc5.Microscopy.Com
} From: "Buddy Steffens" {STEFFENS.B-at-calc.vet.uga.edu}
} Organization: College of Vet. Med
} To: gwe-at-biotech.ufl.edu (Greg Erdos), Microscopy-at-Sparc5.Microscopy.Com
} Date: Fri, 17 May 1996 12:48:18 EST
} Subject: Re: Service contracts w\ CIC Agency
} Priority: normal
}
} This is in response to Greg Erdos' message regarding CIC Agency.
}
} I was "encouraged" last year by our college's administrators to put
} our 2 scopes on contract w\ CIC. I am approaching the 8 month period
} now since I did include our new SEM on the contract. The Veterinary
} teaching hospital here, as well as some other labs are also using
} this company. Here are some of our observations/experiences.
}
} CIC Agency is a Risk Management Corporation...essentially an
} insurance company. Instead of contracting with your regular vendor
} for preventative maintenance, ie JEOL, Phillips, etc., you contract
} with CIC. Their charge for the service is typically 20-30% below
} your vendor (its 26% savings on our SEM over JEOL). They include 2
} preventative maintenances per year. When you need service, you call
} whoever you want (ie JEOL) and schedule emergency service. The
} service engineer orders the parts, does the work as usual, and hands
} you a bill for parts, labor, and whatever else they charge for. You
} send the bill to CIC and they pay it. The only stipulation is that
} you get approval from them if the bill is expected to be over $5000.
}
} Some of the folks here who are using them for hospital equipment are
} not too pleased and are talking about switching back. I haven't
} decided yet, but am sure that I won't be putting my TEM with them
} this upcoming year.
}
} I would be happy to expound on this a bit more with anyone who is
} interested.
}
}
}
}
}
} W. L. Steffens, Ph.D
} Dept. of Veterinary Pathology
} College of Veterinary Medicine
} University of Georgia
} Athens, GA 30602
} STEFFENS.B-at-CALC.VET.UGA.EDU
} Voice: (706) 542-5536
} FAX: (706) 542=5828
}
}





From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Tue, 21 May 1996 21:51:01 -0500
Subject: MMMS Specimen Prep Workshop at ANL

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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Workshop on Specimen Preparation for Transmission Microscopy
in The Physical and Life Sciences

Sponsored by

Midwest Microscopy & Microanalysis Society (MMMS)

June 7 & 8, 1996

____________
Location
____________
Argonne National Laboratory
Materials Science Division
Building 212
9700 S. Cass Ave
Argonne, Illinois 60439

Organizer: Nestor J. Zaluzec

____________
Dates & Times
____________

June 7 - Physical Sciences
10 am - 4 pm

June 8 - Life Sciences
10 am - 4 pm

AM - Plenary Invited Lectures
PM - Hands on Workshop/Lab

____________
Registration
____________

All visitors must Pre-Register and obtain a site pass
for entry to Argonne National Laboratory. Call
Sheila Jungman at (708)-252-4987 or register
electronically on the WWW at

http://www.msa.microscopy.com/MSALAS/MMMSJune.html

____________
Fees
____________

MMMS Members - None
NonMembers - $10.00
Students - $5.00

Registration Fees are payable at the meeting.

__________________
Meeting Format
___________________


The meeting will consist of morning lectures followed
by afternoon hands on workshops. All presentations will
be by invited speakers. An exhibit area will be available
for Corporate Members.

______________________
Workshop Program
______________________
Physical Sciences
______________________

* Electro-Chemical Polishing
B. Kestel - ANL

* Ion Milling, Mechanical Thinning, & Cross-sectioning
R. Alani - Gatan Inc.

* Microtomy of Hard Materials
P. Swab-Adv. Refract. Tech.

__________________
Life Sciences
__________________

* Introd. to Staining and Fixation
G. Scott - Univ. of Wisc.

* Immuno-Gold Labeling
R. Albrecht- Univ. of Wisc.

* Microwave Fixation Techniques
L. Dickey - Micro Lines Mrkt.

* CryoMicrotomy for Immunocytochemical Studies
M. Wilhite - Consultant









From: Sara Miller :      saram-at-acpub.duke.edu
Date: Tue, 21 May 1996 10:25:47 -0400 (EDT)
Subject: Re: Service contracts

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Thanx for your comments. One question that comes to mind is whether
there will be a special charge from the microscope companies to re-enter
their service contract program if the cheaper service doesn't work out.
It still may be worth it, but the figures are things one should know up
front before switching.
Sara


On Mon, 20 May 1996 amorr-at-mse.ufl.edu wrote:

} Date: Mon, 20 May 96 13:16:15 EST
} From: amorr-at-mse.ufl.edu
} To: Sara Miller {saram-at-acpub.duke.edu} , EMLAB-at-OPUS.MCO.EDU
} Cc: microscopy-at-Sparc5.Microscopy.Com
} Subject: Re: Service contracts
}
} Greg, Sara, Ed:
} Changes always give us feelings of uncertainty and thus we
} resist them. Greg's initiative will give us insight through
} responses from people who used this type of service
} contract companies before, and hence will alleviate those
} fears. Another source of information are the
} "orientation" meetings that these companies are giving to
} prospective clients. Although I missed one such meeting, I
} heard some interesting comments from someone who
} attended. Based on those comments, I would like to
} add to Ed's and Sara's response to Greg that we
} should be informed on what each company is offering under
} the guidelines of the client institution (say, our
} university). In this particular case, the company (that I
} believe already won the univ. bid) does not propose to have
} their own repair engineers do the work, but rather gives us
} the choice of whom to call. So, we can still call JEOL to
} repair our Jeol EMs, Hitachi to fix our Hitachi instruments,
} etc (or, more ineterstingly, the other way around).
} Also, if this does not work out, we can discontinue the
} service and go back to our present service contracts. A
} big point in favor of the new system is that it is cheaper,
} and the prices are guaranteed for 2 years.
}
} I look forward to see more posting on this issue.
}
} Augusto Morrone
} Univ. of Florida
} MSE
}




From: Larry Maser :      lmaser-at-mbl.edu
Date: Wed, 22 May 1996 01:10:54 -0400 (EDT)
Subject: Happy Birthday to John H.L. Watson

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Dear Friends,

John H.L. Watson will celebrate his 80th Birthday on Monday, May 27th.
I'd like to suggest that we all send him Birthday cards:

John H.L. Watson
652 Hupp Cross
Bloomfield Village, MI 48301-2434

Sincerely,

Larry Maser





From: paulc-at-gps.caltech.edu (Paul K. Carpenter)
Date: Tue, 21 May 1996 17:15:19 -0700
Subject: Re: EDS System Selection (longish)

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It is nice to see a thread on energy-dispersive spectrometry get going
here. The original question dealt with selecting an EDS system from the
current systems available. There is no question that some really neat
advances have been made in the hardware arena for pulse processing etc. I
am not really qualified to comment on such things.

However, as a user of EDS systems for 13-odd years, I do have a few
observations made as an analyst in the lab. I'm intentionally taking the
devils advocate point of view here, and welcome corrections and comments.

First, those who used Tracor-Northern systems for EDS analysis obtained the
Flextran code (interpreted Basic/Fortran sort of), and one could educate
oneself as to exactly how you started with an EDS spectrum, obtained the
fitted peak areas by least-squares, and finally got the concentrations of
the elements after ZAF correction. When you bought the system, you got the
source code. Today you will find that almost nobody will give you the very
code that is used to do all this; you may find that it is not so easy to
get explicit details of all the processing and correction algorithms
(exactly whose version of ZAF or PRZ is being used?). In a nutshell, the
software is not available to the user. The user can neither learn about
all the details of the algorithm, nor add to it with code that they have
written themselves (with the exception of macros or command sequences,
which just automate procedures, mainly).

Secondly, most turnkey systems are demonstrated, purchased, and used to do
so-called "standardless analysis", whereby either stored library EDS
reference spectra are used (obtained typically on another instrument,
likely at different take-off angle and accelerating voltage than what you
are using to do your analyses), or fundamental parameters algorithms are
used to calculate the spectra that would represent actual standards.
Clearly, it is easier to acquire a spectrum on your sample and turn it over
to the standardless software and let it do its magic. However, you
implicitly accept errors possibly as large as 100% (!) due primarily to
imperfections in the fundamental parameters equations and/or data. One is
better off acquiring spectra on actual standards and fitting the sample
spectra to those spectra acquired on the same instrument under the same
conditions. But even in state of the art commercial systems, this
procedure is convoluted and is definitely not within the realm of the
casual user. And I have attended many a demo.

I hope we all agree that the goal is to perform quantitative EDS analysis.
If not, then one can simply acquire spectra on different parts of (say) a
failed solder joint, tell the client "looks like more Sn over here than
over there" and be done with it. Many questions might be answered with
this qualitative style of analysis. One can find commercial PC-based
systems that allow you to acquire EDS spectra, but perhaps either don't do
least-squares fitting and ZAF correction (cheaper systems), or only do the
standardless thing (more expensive, generally). Many software packages do
not even print out the fitted peak area and/or k-ratio (only the final
analysis), and almost none propagate the counting statistics error from the
peak measurement to the concentration units of the resulting analysis. For
standardless analysis packages, many do not show you the normalization
factor that was used, but we don't care 'cause those 100% totals every time
sure look great. You know, users now pay up to several hundred dollars per
hour for SEM/EDS time. Educated clients ask questions like "what is the
concentration of Fe here and there, what is the precision of measurement
for Fe vs. Mg, is Cr below the detection limit, and what is the accuracy of
the analysis", and I'll bet that analysts outfitted with the current
state-of-the-art systems can't answer those questions. (Not to say that
older systems gave you this information either, it just has yet to be
deemed as necessary information).

The trend is toward black-box configurations that are easier to use and
produce a result that acceptable for typical applications --
understandably. EDS vendors have had to sell systems for a fraction of
what they once sold for (and still carry on with development), and this
places really tough constraints on what they can deliver. I admire them
for toughing it out, and I know from dealing with EDS software that the
code can get really complex and requires much work to develop. DTSA is a
case in point; this program is arguably 5 years old and is still being
tuned up because it is big big BIG.

My contention is that while hardware advances have been made, it is the
software that is now hobbling quantitative EDS analysis.

Tell me this: who has an EDS system that can obtain a digital image of a
sample where a full least-squares fit and ZAF correction has been done on
the fly at each pixel, and the resulting image portrays the concentration
of the element mapped (not a scaled x-ray intensity map). I've seen papers
at national meetings describing this capability, as far back as 5-10 years
ago. The limitation for sure isn't the processing speed of the computers
now available, or the amount of RAM or hard drive storage space. How hard
can it be to do a complete analysis at each pixel in an image?

Remember, our goal was to do quantitative EDS.

Paul Carpenter



+----------------------------------------------------+
| Paul K. Carpenter paulc-at-gps.caltech.edu |
| Division Analytical Facility |
| Geological and Planetary Sciences MC 170-25 |
| California Institute of Technology |
| Pasadena, CA 91125 |
| 818-395-6126 (X-ray Lab) 818-568-0935 (Dept. FAX) |
+----------------------------------------------------+






From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Wed, 22 May 1996 7:45:25 -0400
Subject: Re: MacElwain Tissue Chopper: Request for Info

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Message-ID: {B3D3A23101F70300-at-mhs.unc.edu}
In-Reply-To: {A6D3A23101F70300}

When Sorval got out of the microscopy business It sold off the
'Histology' line to Polaron/BioRad and the 'EM' line to RMC. I believe
that if you contact Steven Slapp at 75767 -at- SMTP (ENERGY BEAM SCIENCES,
INC) {75767.640-at-CompuServe.COM} he may be abel to help you.
regards,

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: Walter A. Mannheimer :      wamann-at-metalmat.ufrj.br
Date: Wed, 22 May 1996 10:30:09 EST3EDT
Subject: meeting in Brazil

Contents Retrieved from Microscopy Listserver Archives
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Thanks to those who have shown their interest in the

5 th. Brazilian Conference on Microscopy of Materials
MICROMAT96
Rio de Janeiro October 13-16, 1996

Please note that there has been a change in the email address

sbme-at-dcmm.puc-rio.br

greetings to all
Prof.Walter A.Mannheimer
Metallurgy and Materials Engineering
Federal University of Rio de Janeiro
POBox 68505 21945 Rio de Janeiro, Brazil
Voice 5521 280-7443 (Dept.office) 5521 590-0579 (direct)
Fax 5521 290-6626 Email: wamann-at-metalmat.ufrj.br




From: Dr. Andrew P. Somlyo :      aps2n-at-elvis.med.virginia.edu
Date: Wed, 22 May 1996 09:44:03 -0400 (EDT)
Subject: Re: EDS System Selection (longish)

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Amen. We are discarding our (expensive, nearly useless and
unsupported) commercial software and rewriting our old (Fortran) code
that did most of what is described by Paul Carpenter.

On Tue, 21 May 1996, Paul K. Carpenter wrote:

}
} It is nice to see a thread on energy-dispersive spectrometry get going
} here. The original question dealt with selecting an EDS system from the
} current systems available. There is no question that some really neat
} advances have been made in the hardware arena for pulse processing etc. I
} am not really qualified to comment on such things.
}
} However, as a user of EDS systems for 13-odd years, I do have a few
} observations made as an analyst in the lab. I'm intentionally taking the
} devils advocate point of view here, and welcome corrections and comments.
}
} First, those who used Tracor-Northern systems for EDS analysis obtained the
} Flextran code (interpreted Basic/Fortran sort of), and one could educate
} oneself as to exactly how you started with an EDS spectrum, obtained the
} fitted peak areas by least-squares, and finally got the concentrations of
} the elements after ZAF correction. When you bought the system, you got the
} source code. Today you will find that almost nobody will give you the very
} code that is used to do all this; you may find that it is not so easy to
} get explicit details of all the processing and correction algorithms
} (exactly whose version of ZAF or PRZ is being used?). In a nutshell, the
} software is not available to the user. The user can neither learn about
} all the details of the algorithm, nor add to it with code that they have
} written themselves (with the exception of macros or command sequences,
} which just automate procedures, mainly).
}
} Secondly, most turnkey systems are demonstrated, purchased, and used to do
} so-called "standardless analysis", whereby either stored library EDS
} reference spectra are used (obtained typically on another instrument,
} likely at different take-off angle and accelerating voltage than what you
} are using to do your analyses), or fundamental parameters algorithms are
} used to calculate the spectra that would represent actual standards.
} Clearly, it is easier to acquire a spectrum on your sample and turn it over
} to the standardless software and let it do its magic. However, you
} implicitly accept errors possibly as large as 100% (!) due primarily to
} imperfections in the fundamental parameters equations and/or data. One is
} better off acquiring spectra on actual standards and fitting the sample
} spectra to those spectra acquired on the same instrument under the same
} conditions. But even in state of the art commercial systems, this
} procedure is convoluted and is definitely not within the realm of the
} casual user. And I have attended many a demo.
}
} I hope we all agree that the goal is to perform quantitative EDS analysis.
} If not, then one can simply acquire spectra on different parts of (say) a
} failed solder joint, tell the client "looks like more Sn over here than
} over there" and be done with it. Many questions might be answered with
} this qualitative style of analysis. One can find commercial PC-based
} systems that allow you to acquire EDS spectra, but perhaps either don't do
} least-squares fitting and ZAF correction (cheaper systems), or only do the
} standardless thing (more expensive, generally). Many software packages do
} not even print out the fitted peak area and/or k-ratio (only the final
} analysis), and almost none propagate the counting statistics error from the
} peak measurement to the concentration units of the resulting analysis. For
} standardless analysis packages, many do not show you the normalization
} factor that was used, but we don't care 'cause those 100% totals every time
} sure look great. You know, users now pay up to several hundred dollars per
} hour for SEM/EDS time. Educated clients ask questions like "what is the
} concentration of Fe here and there, what is the precision of measurement
} for Fe vs. Mg, is Cr below the detection limit, and what is the accuracy of
} the analysis", and I'll bet that analysts outfitted with the current
} state-of-the-art systems can't answer those questions. (Not to say that
} older systems gave you this information either, it just has yet to be
} deemed as necessary information).
}
} The trend is toward black-box configurations that are easier to use and
} produce a result that acceptable for typical applications --
} understandably. EDS vendors have had to sell systems for a fraction of
} what they once sold for (and still carry on with development), and this
} places really tough constraints on what they can deliver. I admire them
} for toughing it out, and I know from dealing with EDS software that the
} code can get really complex and requires much work to develop. DTSA is a
} case in point; this program is arguably 5 years old and is still being
} tuned up because it is big big BIG.
}
} My contention is that while hardware advances have been made, it is the
} software that is now hobbling quantitative EDS analysis.
}
} Tell me this: who has an EDS system that can obtain a digital image of a
} sample where a full least-squares fit and ZAF correction has been done on
} the fly at each pixel, and the resulting image portrays the concentration
} of the element mapped (not a scaled x-ray intensity map). I've seen papers
} at national meetings describing this capability, as far back as 5-10 years
} ago. The limitation for sure isn't the processing speed of the computers
} now available, or the amount of RAM or hard drive storage space. How hard
} can it be to do a complete analysis at each pixel in an image?
}
} Remember, our goal was to do quantitative EDS.
}
} Paul Carpenter
}
}
}
} +----------------------------------------------------+
} | Paul K. Carpenter paulc-at-gps.caltech.edu |
} | Division Analytical Facility |
} | Geological and Planetary Sciences MC 170-25 |
} | California Institute of Technology |
} | Pasadena, CA 91125 |
} | 818-395-6126 (X-ray Lab) 818-568-0935 (Dept. FAX) |
} +----------------------------------------------------+
}
}
}




From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Wed, 22 May 1996 11:23:41 -0400 (EDT)
Subject: TEM Screen Coating

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To all interested:

As I said I used green phosphor from JEOL, cat. # 423-011. Call and see
if this number still holds true.

You will need a collodion solution: 4 grams parlodian
25 ml absolute ethel alcohol
75 ml ether

You will also need a dish big enough for the screen and a cover
(preferably glass) with a small hole for removal of the liquid.


1. Remove the old coating from the screen by washing in acetone. The
cleaned plate must be free from all particles of matter and the surface
must be free from blemishes and scratches.

2. Prepare a sufficient volume of 4% (w/v) suspension of phosphor powder
in acetone containing about 1% collodion. The total volume must be
sufficent to fill selected dish with liquid to a depth of about 1cm above
the surface of the screen in position on the bottom of the dish.

3. Agitate the suspension vigorously (I used sonicator) then pour it
rapidly into the dish.

4. Wait about 5-10 seconds to allow larger particles to settle and
swirling to cease.

5. Slide the plate smoothly into the liquid, preferably without scraping
the bottom of the dish.

6. Cover the dish and leave to settle. When the suspension has settled
and the remaining liquid is clear, draw off the liquid by inserting a
suction tube through the previously prepared hole in the lid of the
dish. It is extremely important not ot disturb the screen plate or the
liquid above it in any way as this is done. Draw off the liquid steadily
and slowly then remove the suction tube.

7. Leave the screen to dry without any disturbance of any kind. Do not
lift the lid to inspect the screen until the powder is quite dry because
a slight change in drying conditions can produce a visible mark on the
damp surface.

8. When the powder is quite dry, remove the screen and wipe off any
excess phosphor from the back and sides of the screen.

9. Install.

10. A newly coated screen will outgas for a short time when it is first
placed in the microscope. Pumping times may therefore be a little longer
than normal at first.

A very small particle size is desirable for high resolution screens and
it may be advantageous to agitate the the phosphor suspension in an
ultrasonicator before putting it into the dish.

Hope this helps. It can get tricky and you may not get it the first time.
Patience really helps!

Peace,

Phil Rutledge




From: paulc-at-gps.caltech.edu at hubsmtp
Date: 05/22/96 12:54 AM
Subject: Re: EDS System Selection (longish)

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I have used a package produced by Oxford for the EXL series called QMAP that
does perform a multiple least squares fit and quantitative analysis (bulk or
thin film) on EDS spectra obtained at every pixel of a map. I have not used it
for a few years, but it was very nice when trying to obtain maps from overlapped
peaks in the EDS spectra.

I have no financial interest in Oxford, besides the fact that I am a customer.

Joe Michael
_______________________________________________________________________________

It is nice to see a thread on energy-dispersive spectrometry get going
here. The original question dealt with selecting an EDS system from the
current systems available. There is no question that some really neat
advances have been made in the hardware arena for pulse processing etc. I
am not really qualified to comment on such things.

However, as a user of EDS systems for 13-odd years, I do have a few
observations made as an analyst in the lab. I'm intentionally taking the
devils advocate point of view here, and welcome corrections and comments.

First, those who used Tracor-Northern systems for EDS analysis obtained the
Flextran code (interpreted Basic/Fortran sort of), and one could educate
oneself as to exactly how you started with an EDS spectrum, obtained the
fitted peak areas by least-squares, and finally got the concentrations of
the elements after ZAF correction. When you bought the system, you got the
source code. Today you will find that almost nobody will give you the very
code that is used to do all this; you may find that it is not so easy to
get explicit details of all the processing and correction algorithms
(exactly whose version of ZAF or PRZ is being used?). In a nutshell, the
software is not available to the user. The user can neither learn about
all the details of the algorithm, nor add to it with code that they have
written themselves (with the exception of macros or command sequences,
which just automate procedures, mainly).

Secondly, most turnkey systems are demonstrated, purchased, and used to do
so-called "standardless analysis", whereby either stored library EDS
reference spectra are used (obtained typically on another instrument,
likely at different take-off angle and accelerating voltage than what you
are using to do your analyses), or fundamental parameters algorithms are
used to calculate the spectra that would represent actual standards.
Clearly, it is easier to acquire a spectrum on your sample and turn it over
to the standardless software and let it do its magic. However, you
implicitly accept errors possibly as large as 100% (!) due primarily to
imperfections in the fundamental parameters equations and/or data. One is
better off acquiring spectra on actual standards and fitting the sample
spectra to those spectra acquired on the same instrument under the same
conditions. But even in state of the art commercial systems, this
procedure is convoluted and is definitely not within the realm of the
casual user. And I have attended many a demo.

I hope we all agree that the goal is to perform quantitative EDS analysis.
If not, then one can simply acquire spectra on different parts of (say) a
failed solder joint, tell the client "looks like more Sn over here than
over there" and be done with it. Many questions might be answered with
this qualitative style of analysis. One can find commercial PC-based
systems that allow you to acquire EDS spectra, but perhaps either don't do
least-squares fitting and ZAF correction (cheaper systems), or only do the
standardless thing (more expensive, generally). Many software packages do
not even print out the fitted peak area and/or k-ratio (only the final
analysis), and almost none propagate the counting statistics error from the
peak measurement to the concentration units of the resulting analysis. For
standardless analysis packages, many do not show you the normalization
factor that was used, but we don't care 'cause those 100% totals every time
sure look great. You know, users now pay up to several hundred dollars per
hour for SEM/EDS time. Educated clients ask questions like "what is the
concentration of Fe here and there, what is the precision of measurement
for Fe vs. Mg, is Cr below the detection limit, and what is the accuracy of
the analysis", and I'll bet that analysts outfitted with the current
state-of-the-art systems can't answer those questions. (Not to say that
older systems gave you this information either, it just has yet to be
deemed as necessary information).

The trend is toward black-box configurations that are easier to use and
produce a result that acceptable for typical applications --
understandably. EDS vendors have had to sell systems for a fraction of
what they once sold for (and still carry on with development), and this
places really tough constraints on what they can deliver. I admire them
for toughing it out, and I know from dealing with EDS software that the
code can get really complex and requires much work to develop. DTSA is a
case in point; this program is arguably 5 years old and is still being
tuned up because it is big big BIG.

My contention is that while hardware advances have been made, it is the
software that is now hobbling quantitative EDS analysis.

Tell me this: who has an EDS system that can obtain a digital image of a
sample where a full least-squares fit and ZAF correction has been done on
the fly at each pixel, and the resulting image portrays the concentration
of the element mapped (not a scaled x-ray intensity map). I've seen papers
at national meetings describing this capability, as far back as 5-10 years
ago. The limitation for sure isn't the processing speed of the computers
now available, or the amount of RAM or hard drive storage space. How hard
can it be to do a complete analysis at each pixel in an image?

Remember, our goal was to do quantitative EDS.

Paul Carpenter



+----------------------------------------------------+
| Paul K. Carpenter paulc-at-gps.caltech.edu |
| Division Analytical Facility |
| Geological and Planetary Sciences MC 170-25 |
| California Institute of Technology |
| Pasadena, CA 91125 |
| 818-395-6126 (X-ray Lab) 818-568-0935 (Dept. FAX) |
+----------------------------------------------------+




From: ebs-at-ebsciences.com
Date: Wed, 22 May 1996 13:41:03 -0500
Subject: Re: MacElwain Tissue Chopper: Request for Info

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The old Dupont-Sorvall TC-2 Tissue Chopper unfortunately got lost in the
shuffle when BioRad purchased their JB-4 and RMC their ultramicrotomes. It
is no longer made, although used TC-2s can sometimes be found.

The McIlwain Tissue Chopper is distributed in the U.S. by Brinkmann
Instruments (phone: 516-334-7500, fax: 516-334-7506).

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: wise-at-vaxa.cis.uwosh.edu
Date: Wed, 22 May 1996 14:34:06 +0000
Subject: Re: MacElwain Tissue Chopper: Request for Info

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To all,

I am trying to model a complex, interconnected membrane structure
in three dimensions. If I can hand-measure dimensions off of micrographs,
is there a computer program into which I can enter those numbers and then
draw a "rotatable", 3-D image? I know that such a capability exists on
some confocal systems but they have several million data bits to deal with.
I would probably only have a hundred or so measurements. Am I asking the
right question? Let me ask it this way: is there software available that
would allow someone to scan TEMs of serial sections and then display a
registered, 3-D image? Is this similar to what confocal scopes do?

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: Liang, Long :      LLIANG-at-is.arco.com
Date: 22 May 1996 13:36:13 CST
Subject: Hitachi Pump Suppliers ?

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Message-Id: {MACMS.LLIANG.555206150096143FMACMS-at-IS.ARCO.COM}

Dear Microscopists,

After being used about 10 years, my Hitachi direct-drive rotary vacuum
pump (model 160VP CuteVac for ISI DS-130 SEM) started making a rumbling
noise this morning. The oil level is OK and the oil is clean. The SEM
is functional but I think this is the first symptom of a future pump
problem.

Does anyone know of any HItachi rotary pump suppliers in the US ? Thanks
for your information.

Long Liang
ARCO EPMA/SEM Lab
Plano, TX







From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Wed, 22 May 1996 11:00:55 -0800
Subject: bubbles in cryoholder

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hello Philip:

we had problems with bubbles in the area of the specimen insertion of the
workstation (our device is several years old, not one of the newer units).
After playing around with it for several months, we bit the bullet and sent
the sample holder back for assessment. Gatan told us it was a problem in
the cryounit itself, not the workstation. From their description of the
problem, our bubble problem is not an uncommon occurrence in the older
units and was caused by a leak in the specimen arm. Our sampleholder in
the same workstation worked fine after they rebuilt it and added new
zeolite. The repair ran 1-2K.

good luck with it.

steve


} Hello everybody,
}
} we have problems with boiling nitrogen in the workstation of a Gatan
} cryo holder. Particularly large bubbles form near the specimen making
} transfer very difficult. Has anybody had that problem and found a
} solution.
}
} Thanks in advance,
}
} Philip

Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego, CA 92182-4614
phone: (619)594-4523
fax:(619)594-5676
email:sbarlow-at-sunstroke.sdsu.edu






From: Gillian Bond :      gbond-at-mailhost.nmt.edu
Date: Wed, 22 May 1996 16:25:29 -0600 (MDT)
Subject: TEM specimen prep - need ion mill

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We have urgent need of an ion mill (used). We also find ourselves with a
sum of money for equipment upgrade that we must either use or lose by
June 30th. Please can anyone help?







From: jgilkey-at-ccit.arizona.edu (John C. Gilkey)
Date: Wed, 22 May 1996 11:13:58 -0700
Subject: Re: TEM - Gatan cryo holder

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} we have problems with boiling nitrogen in the workstation of a Gatan
} cryo holder....

Before transferring the grid into the holder, we drain excess LN2 from
the crytotransfer system by backing the stage a couple of centimeters out
of the transfer device, until the flow of LN2 dribbling out of the port is
low, then reseating the stage. The LN2 level is then just below the level
of the stage, and the upper reservoir is still filled. We have encountered
no problems with devitrification or frost when transferring the specimens
to the stage through the cold nitrogen gas above the stage, so long as the
transfer is made quickly with forceps that are adequately precooled (and
kept cool by periodic immersion in the reservoir, should the grid need to
be centered in the stage). We do this because we found that (a) loading
was very difficult if the LN2 was at or above the level of the stage, and
(b) the specimens often seemed to have more frost on them when they were
transferred through the LN2.






From: alan.wilson-at-dsto.defence.gov.au (Alan Wilson)
Date: Thu, 23 May 1996 09:51:33 +1000
Subject: Re: distilled water corrodes copper, TEM cooling

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} At 9:55 AM 5/21/96, melvyn dickson wrote:
} } Distilled (and I imagine de-ionised) water has a more corrosive effect on
} } copper piping than water with additive. Can't give a reference at this date
} } but this was in the (engineering?) literature back in 1967. So we always use
} } additives for fear of corroding cooling pipes inside lenses.
} }
}
} That appeared to be the case for our JEM-4000EX. We use double distilled
} water for the lens cooling. The cooper rust built-up was just unbelievable
} we found out last year when we had to change the cooling water. The fluid
} was so bad that I saved a vial for teaching purposes. I guess the ion
} concentration in the fluid must be balanced so that it does not leave any
} deposite and yet it does not try to take too many ions from the cooper
} pipes.
}

Perhaps another caveat regarding out use of de-ionized water. The system
is very stable and we have not changed the water for years. Perhaps it has
reached an equilibrium if corrossion of copper with de-ionized (not
distilled) water is a problem. Also, there is some clear tubing in our
system with no evidence of water discolouration.

alan.wilson-at-dsto.defence.gov.au

Dr Alan Wilson
Senior Research Scientist
Ship Structures and Materials Division
Aeronautical and Maritime Research Laboratory
Defence Science and Technology Organization
506 Lorimer St
Fishermens Bend 3207
Victoria Australia
ph 61 3 9626 7508, fax 61 3 9626 7816 or 61 3 9626 7087






From: em-at-mediacity.com (Ed Monberg)
Date: Wed, 22 May 1996 18:44:58 -0800
Subject: RE: Used equipment suppliers

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} The classified ads in The Scientist include a lot of used equipment vendors.
} The most recent issue also includes a web address (http://www.labx.com) and
} indicates that you can find information from multiple vendors. I haven't
} checked it out yet myself.
}
} Susan Udin
} Dept. of Physiology
} SUNY, Buffalo NY


etc., etc.,



WE supplu used equipment !

- - and much more than we have had time to list.


(Have at us dear "Microscopy.Com" members)








Regards,



(signed) Ed Monberg {em-at-mediacity.com}

--------------------------------------------------

510-429-1060 Fax 429-1065
LMDC, (Laser Motion Development Co.)
3101 Whipple Road
Union City, CA 94587-1216


For our Most recent Catalogue of "On Hand" EQUIPMENT:
Send empty mail to: {Cat-at-lasermotion.com}


Our web page: http://www.lasermotion.com (Is beginning to take shape!)
Our e-mail: office-at-lasermotion.com

{-------------------------------- Our page width
-----------------------------}






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Wed, 22 May 1996 19:52:21 -0800
Subject: Re: Hitachi Pump Suppliers ?

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Dear Long,
I would imagine that the NSA (Nissei Sangyo America) company that sells
Hitachi microscopes would sell the rotary pumps, since that pump is
supplied on all their microscopes.
I have had the same problem on my Hitachi rotary pumps and it turned out to
be deterioration of the rubber coupler between the motor and pump. This was
simple and cheap to fix myself and I keep a supply of them for when a pump
gets noisy. If the pump starts to leak oil, then I replace the two shaft
seals inside the pump, about a 1/2 hour job.
The three pumps on my TEM have been going constantly for 131/2 years now.
Regards,
Mary Mager

Long Liang wrote:
} Dear Microscopists,
}
} After being used about 10 years, my Hitachi direct-drive rotary vacuum
} pump (model 160VP CuteVac for ISI DS-130 SEM) started making a rumbling
} noise this morning. The oil level is OK and the oil is clean. The SEM
} is functional but I think this is the first symptom of a future pump
} problem.
}
} Does anyone know of any HItachi rotary pump suppliers in the US ? Thanks
} for your information.
}
} Long Liang
} ARCO EPMA/SEM Lab
} Plano, TX

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: John Best :      jbest-at-vicon.net
Date: Tue, 21 May 1996 18:10:08 -0700
Subject: Re: EDS System Selection

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Message-ID: {31A26970.49F9-at-vicon.net}

Rick, Peter, and All,

Rick, you're a gentleman.

I should have been a little more thoughtful in extrapolating my
experience with DSP's into the EDS arena. As Rick and Peter point out
with only the experience EDS design engineers could, there are certain
things mother nature just won't let us do.

However; I'll remain firm in my prediction that the digital front end
will do more for EDS systems than the combined innovations of the last 10
years.

I'd like to introduce a new subject if I may. Would anyone care to
comment on the ramifications to the end user if SEM manufacturers would
agree to implement a standard interface for control of the beam by EDS
systems?

Warm regards to all.
John Best.






From: John Best :      jbest-at-vicon.net
Date: Wed, 22 May 1996 23:49:06 -0700
Subject: EDS evolution thread

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Message-ID: {31A40A62.6BB-at-vicon.net}

Paul Carpenter and all listening to the EDS thread,

You've asked many good questions. It sounds like you've gotten quite a
few frustrations off your chest at once! :)

I'm going to comment on just a few this time.

You write: I hope we all agree that the goal is to perform quantitative
EDS analysis.

I'd respond, for some (mabey a majority) of us. I know of many
situations in which it is sufficient to say what is present, even if I
couldn't get the exact concentration. Also, in mapping situations, it's
been sufficient to tell where something is, although I couldn't say
exactly how much. I'd guess you must be involved in a critical process
control application.

You raise some much larger questions regarding the evolution of systems
and the accessability of an interested end-user to their inner workings.
I think this could be a touchy issue with manufacturers, so I'm going to
give it some thought before responding. But I agree with you that in
general I don't like "point and shoot" type of instruments. This
approach is nice in some situations, but us hard core users have lost
some flexability. I'll broaden it a bit and ask: how could the SEM, EDS,
and IA systems manufacturers provide some standardization (without
suffering commercially) and provide end users with a common interface to
control low level functionality of the various instruments?

Your last couple of paragraphs ring similar to a comment I made a few
days ago. I think the manufacturers have been led around by users who
are more interested in what operating system is being used than what the
system can actually do. Not that having compatability with a particular
computing environment isn't important, but it seems the evolution of some
very nice software packages was cut short whilst everyone jumped on the
Windows bandwagon and scrambled (spent a lot of programmers hours) to
rewrite the code for more basic functionality of the system. Whew.

That's probably why PDP-11 based systems hung around for so long. I'll
bet you can still find PDP-11 based EDS systems that can do a lot of
things many of the new Windows and Mac based systems can't. How do we
avoid this waste the next time around? Mabey all EDS and SEM related
software can be written in JAVA.

Regards to all................. JB






From: dietmar.reiter-at-uibk.ac.at (Dietmar Reiter)
Date: Thu, 23 May 1996 11:24:36 +0100
Subject: Re: rotatable 3D image

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} To all,
}
} I am trying to model a complex, interconnected membrane structure
} in three dimensions. If I can hand-measure dimensions off of micrographs,
} is there a computer program into which I can enter those numbers and then
} draw a "rotatable", 3-D image? I know that such a capability exists on
} some confocal systems but they have several million data bits to deal with.
} I would probably only have a hundred or so measurements. Am I asking the
} right question? Let me ask it this way: is there software available that
} would allow someone to scan TEMs of serial sections and then display a
} registered, 3-D image? Is this similar to what confocal scopes do?

Robert -

I use NIH Image to accomplish some of these tasks (serial TEM section
reconstruction, distortion correction, etc), starting with drawing outlines
from scanned TEM negatives.
Image comes with built in registration capabilities, but you easily can
enhance the functionality (and interactivity needed) with macros. Lets say,
you generate a macro, which allows you to registrate/measure some points on
your image (thresholded, manually drawn, whatever), save these measured
points as coloured points on extra images, have these images a few times
duplicated (always in mind the scale calibration) to get real 3d objects of
your measured points, have them projected with lots of angle and
transparency paramters, and save them as pics image stacks. Animate them.
Very handy.
However - Image does NOT allow no realtime interactive rotation of the
projected images.

Dietmar

+++ Dietmar Reiter {dietmar.reiter-at-uibk.ac.at} ++++++++++++++++
+++ Dept. of Zoology and Limnology, University of Innsbruck ++++
+++ Technikerstrasse 25, A - 6020 Innsbruck, Austria +++++++++
+++ ph: (+43)-512-507-6170 (-6161), fax: ..-507-2930 (-2957) +++






From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Thu, 23 May 1996 6:20:26 -0400
Subject: unsubscribe

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Message-ID: {7823A43101F70300-at-mhs.unc.edu}

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: Audette, David :      deaudette-at-corp.olin.com
Date: Thu, 23 May 1996 08:46:00 -0500
Subject: Confocal or microprobe on skin

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I recently was assigned to investigate the utility of confocal and
microprobe (perhaps AFM) microscopy for examining topical applications of
biocides on pig skin samples. I have already performed some SEM/EDS work on
this but now the question is how does the biocide affect the skin surface.
I was hoping someone may know of some similar work in this area.

Thanks in advance,

Dave Audette
Olin Research Center
Cheshire, CT
deaudette-at-corp.olin.com




From: Jason Kalgreen :      Jason.E.Kalgreen-1-at-tc.umn.edu
Date: Thu, 23 May 1996 08:37:31
Subject: unsubscribe

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To: microscopy-at-Sparc5.Microscopy.Com

unsubscribe kalg0001-at-maroon.tc.umn.edu








From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 23 May 1996 11:09:07 -0400
Subject: RE-Corrosion w Deionized wa

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Subject: Time: 10:09 AM
OFFICE MEMO RE:Corrosion w Deionized water Date: 5/23/96

I would be very surprised if either deionized or distilled water is
significantly more corrosive than ordinary tap water in copper and stainless
steel systems.
In a wet environment corrosion (i.e. the dissolving of the metal) always
is a galvanic phenomenon that involves a potential difference between two
parts of the system, an anodic area and a cathodic area. The anodic area is
always more electrochemically reactive than the cathodic area, and the
overall corrosion mechanism consists of four separate processes 1. Metal
atoms dissolve at the anodic area, forming metal ions which go into solution.
2. This reaction releases electrons, which travel through the metal parts
to the cathodic area. 3. At the cathodic area these electrons are consumed
in one or more of a variety of 'cathodic reactions' (in aerated, neutral
solutions such as might typically be found in water chillers this reaction
usually involves the combination of 2 electrons with two molecules of water
and one molecule of oxygen to form 4 hydroxyl ions). 4. There also must be a
flow of ions between the two electrode areas through the water that connects
them to maintain charge neutrality.
Corrosion can be retarded by interrupting any one of these four
processes, and can be enhanced by anything that promotes any of them.
One common cause of accelerated corrosion is having two metals of
considerably different electrochemical activity directly in contact in an
aqueous environment. An example is putting a steel pipe fitting into a
copper water line. The iron is electrochemically more active than the
copper, and so it will become strongly anodic, releases iron ions into
solution, and corroding away rapidly. (The iron ions react with hydroxyl
ions in solution forming a hydrated oron oxide, which we call rust. Rust is
evidence of corrosion, but not the basic problem.) If you put plastic
nipples between the iron and copper components so that there cannot be
electron flow from the iron to copper, corrosion will be prevented.
I suspect that systems filled with deionized or distilled water that
show high rates of discoloration, and other evidence of corrosion, have such
bimetallic couplings that are promoting the corrosion. Under some
circumstances stainless steel is cathodic with respect to copper, and so if a
system has stainless steel parts directly connected to copper parts, so that
there could be a flow of electrons between them, then sufficient galvanic
potential might develop between them to cause the copper to corrode (i.e.
copper ions might dissolve into the solution and cause it to become
discolored). I think this is a more likely explanation for any such
phenomena than the lack of ions in solution in the deionized and distilled
waters, and I would expect that the phenomena would disappear if the system
were rearranged so that the copper and stainless steel parts were no longer
in direct contact.
Ref: Materials Science & Engineering by W. D. Callister, 2nd Ed.,
Wiley, Ch. 18).
W. C. Bigelow (bigelow-at-umich.edu)






From: wise-at-vaxa.cis.uwosh.edu
Date: Thu, 23 May 1996 11:54:06 +0000
Subject: 3D reconstruction

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Thanks to all for the information on computer programs for 3D
reconstructions. Looks like NIH Image is the easiest and cheapest way to
get started in this.

And I was mightily impressed by the 600-700 consecutive serial sections
reported by one respondent!

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: DBRAUER :      dbrauer-at-arserrc.gov
Date: Thu, 23 May 1996 14:13:07 -0500 (EST)
Subject: how to unsubscribe

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MR-Received: by mta CERES.MUAS; Relayed; Thu, 23 May 1996 14:13:07 -0500 (EST)
Disclose-recipients: prohibited

How do I unsubscribe from the microscopy list. I have tried
several different ways and have been unsuccessful.

thanks

dave

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From: Rajesh Patel :      rpatel-at-UMDNJ.EDU
Date: Thu, 23 May 1996 14:54:55 -0400
Subject: aspergillus protocol for tem

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Does anyone have a working protocol for processing aspergillus?

thanks in advance.

rpatel-at-rwja.umdnj.edu





From: John D. Baumstark :      biotech-at-btigate.com -at-btigate.com
Date: Wed, 22 May 1996 15:24:08 -0700
Subject: unsubscribe

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John Baumstark
biotech-at-btigate.com

please unsubscribe




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 23 May 1996 16:19:02 GMT
Subject: DNP Second Ab's

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}
} Colleagues:
}
} I am looking for a source for DNP conjugated secondary
antibodies.
} Can anyone help??
}
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: tania-at-dynamotive.com (Tania Jones)
Date: Thu, 23 May 1996 15:11:18 -0700
Subject: EDX information

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Hi there,

On several occasions I have been asked for literature on the how's and
why's of EDX analysis. Does anyone have any suggestions as to where I
can obtain such material?

Thanks in advance,

Tania Jones
Lab Manager
DynaMotive Technologies





From: ERJ-at-vetmed1.vetmed.ufl.edu
Date: Thu, 23 May 1996 16:02:24 EST
Subject: fixation of buffy coats

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Microscopists, we have had less than favorable results fixing buffy coat of
reptiles (white blood cell fraction) with standard 2.5% glute in 0.1M
cacodylate. Unfortunately we do not know the osmolarity of the blood, though
our experience with other reptiles reveals a significant range within the
same species (wild caught). Does anyone have any suggestions such as
possible additives?

Thanks in advance,

ERJ
Elliott Jacobson
Professor
Department of Small Animal Clinical Sciences
P.O. Box 100126
College of Veterinary Medicine
University of Florida
Gainesville, Florida 32610, USA
Phone: 904-392-4700 X4773
Fax: 904-392-6125
E-Mail: ERJ-at-vetmed1.vetmed.ufl.edu
WEB Site: www.vetmed.ufl.edu






From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Fri, 24 May 1996 11:12:41
Subject: Re: EDX information

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To: Microscopy-at-Sparc5.Microscopy.Com

In article tania-at-dynamotive.com (Tania Jones) writes:
} Date: Thu, 23 May 1996 15:11:18 -0700
} From: tania-at-dynamotive.com (Tania Jones)
} Subject: EDX information

} Hi there,

} On several occasions I have been asked for literature on the how's and
} why's of EDX analysis. Does anyone have any suggestions as to where I
} can obtain such material?

If its still available a very clear intoductory teatment is:

Energy Dispersive X-Ray Microanalysis:
An Introduction
Kevex Instruments Inc., San Carlos CA
Douglas Vaughan, Ed.
printed 1983, 1988, 1989, 199?

Also
X-Ray Microanalysis in the Electron Microscope
J.A. Chandler 1977
a volume in Practical Methods in Electron Microscopy
Audrey Glauert, ED.
Elsevier/North-Holland
ISBN 07204 0607 2

A much fuller treatment:
Scanning Electron Microscopy and X-Ray Microanalysis
2nd Edition
Goldstein, Newbury, Echlin, Joy, Romig, Lyman, Fiori and Lifshin
Plenum Press
ISBN 0-306-44175-6

These are a good start

Mel Dickson.




From: IN% tania-at-dynamotive.com 23-MAY-1996 18:03:30.55
Date: Thu, 23 May 1996 22:15:29 MST/MDT
Subject: RE: EDX information

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Sender: lundm-at-physc1.byu.edu


tania-at-dynamotive.com (Tania Jones) wrote:

`On several occasions I have been asked for literature on the how's and
`why's of EDX analysis. Does anyone have any suggestions as to where I
`can obtain such material?
`
`Thanks in advance,
`
`Tania Jones
`Lab Manager
`DynaMotive Technologies

I have been writing a series of articles on EDX for the newcomer
for Microscopy Today entitled "More than one ever wanted to know
about x-ray detectors." These are available from Microscopy Today,
from me, or maybe best of all, on http://www.MOXTEK.com.

best regards
mark

Mark W. Lund, PhD
Director } } Soft X-ray Web page http://www.moxtek.com { {
MOXTEK, Inc. *************************************************
Orem UT 84057 **"Soft x-rays in the 21st Century" conference **
801-225-0930 ** 8-11 January 1996, Midway Utah **
FAX 801-221-1121 ** http://volta.byu.edu/xray/info.html **
lundm-at-xray.byu.edu *************************************************

"He spoke with a certain what-is-it in his voice, and I could see
that, if not actually disgruntled, he was far from being gruntled."







From: wesleysm-at-biology.und.ac.za (James Wesley-Smith)
Date: Fri, 24 May 1996 08:19:42 +0200
Subject: Re: aspergillus protocol for tem

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Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za}

Hi Rajesh
We have routinely prepared Aspergillus small plugs for TEM using an
overnight fixation in 2.5%GA in phosphate buffer, followed by several buffer
washes and postfixation in 0.5 - 1% osmium tetroxide for 1 hour (all
at 4C). After a distilled-water rinse, en bloc staining/fixation with 0.5%
uranyl acetate in 30% acetone (30 min), followed by graded acetone series,
infiltrated with 50% Spur's resin for 4 hs and overnight in full resin.

Two questions to the forum, though:

1. It is interesting that the cultures will float until
(approx) the 50% acetone step. Although osmium vapour will fix those
exposed areas, I wonder about the role played by GA (or even U Ac)
when it comes to conidiophores and conidia. The results are good anyway.

2. I have know users to favour the use of an equal-parts mixture of GA and
osmium at fairly high concentrations for SEM. How widespread is the
use of this protocol? Given the instantaneous cross-reactivity
between the two fixatives, I really wonder about its usefulness.

Any comments?

James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa





From: Oxford Instruments Pty Ltd :      oisydney-at-ozemail.com.au
Date: Fri, 24 May 1996 19:08:16 +1000 (EST)
Subject: Re: EDS System Selection (longish)

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Dear Paul,
In regard to your question, I would like to point out that the Oxford
Instruments Link ISIS system has a fully quantitative mapping package
(Quantmap). Full spectrum processing (including digital filtering and
fitting) followed by a quantitative matrix correction is performed at each
point. The resulting digital images show elemental concentrations rather
than just a scaled x-ray intensity map. Standard deviation maps are also
produced to allow significance testing.

Your local Oxford rep would be delighted to tell you more!
Best Regards, Julie Sheffield-Parker.

At 05:15 PM 21/05/96 -0700, you wrote:

}
} Tell me this: who has an EDS system that can obtain a digital image of a
} sample where a full least-squares fit and ZAF correction has been done on
} the fly at each pixel, and the resulting image portrays the concentration
} of the element mapped (not a scaled x-ray intensity map). I've seen papers
} at national meetings describing this capability, as far back as 5-10 years
} ago. The limitation for sure isn't the processing speed of the computers
} now available, or the amount of RAM or hard drive storage space. How hard
} can it be to do a complete analysis at each pixel in an image?
}
} Remember, our goal was to do quantitative EDS.
}
} Paul Carpenter
}
}
}
} +----------------------------------------------------+
} | Paul K. Carpenter paulc-at-gps.caltech.edu |
} | Division Analytical Facility |
} | Geological and Planetary Sciences MC 170-25 |
} | California Institute of Technology |
} | Pasadena, CA 91125 |
} | 818-395-6126 (X-ray Lab) 818-568-0935 (Dept. FAX) |
} +----------------------------------------------------+
}
}
}
}


*************************************************
From:-

Julie Sheffield-Parker,
Oxford Instruments Pty. Ltd.,
P. O. Box 7,
Pennant Hills,
NSW 2120,
Sydney, AUSTRALIA

Tel: ++ 61 2 484 6108
Fax: ++ 61 2 484 1667
E-Mail: oisydney-at-ozemail.com.au

*************************************************





From: cytoana-at-univ-lyon1.fr (Mehdi BENCHAIB)
Date: Fri, 24 May 1996 13:30:49 +0200
Subject: Subscribe

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Subscribe Mehdi BENCHAIB

Mehdi BENCHAIB
Laboratoire de Cytologie Analytique
69373 LYON CEDEX 08
E-mail : cytoana-at-univ-lyon1.fr
t=E9l=E9phone : 78 77 70 00 poste 43 23





From: akracher-at-iastate.edu (Alfred Kracher)
Date: Fri, 24 May 1996 08:42:04 -0600
Subject: Os (metal) standard

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X-Sender: akracher-at-pop-1.iastate.edu
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Are there any microprobe (WDS) people who analyze Os alloys (e.g.,
osmiridium)? If so, what standard are you using, and where did you obtain
it?


-----------------------------------------
Alfred Kracher
Geological Sciences
Iowa State University
Ames, IA 50011-3212
akracher-at-iastate.edu
http://www.public.iastate.edu/~akracher
vox:515 294 5439 fax:515 294 6049
-----------------------------------------






From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 24 May 1996 10:46:20 -0400 (EDT)
Subject: Re: Os (metal) standard

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}
} Are there any microprobe (WDS) people who analyze Os alloys (e.g.,
} osmiridium)? If so, what standard are you using, and where did you obtain
} it?
}
Dear Alfred,
I am not doing WDS, nor am I studying alloys, but I made some
Ir and Pt standards by using the acetylacetonate complexes, which dis-
olve in resin. These are available from Strem Chemicals Inc., 7 Muliken
Way, Dexter Industrial Park, Newburyport MA 01950. Alfa Aesar, (800)
343-0660, carries Os, Ir & Pt in many forms--powder, wire, sponge, etc.
I guess these would be better suited for stds for alloys.
Yours,
Bill Tivol




From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Fri, 24 May 1996 11:05:55 -0500
Subject: Re: aspergillus protocol for tem

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Message-Id: {199605241459.KAA17704-at-dogwood.botany.uga.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Aspergillus freezes quite nicely. If you have the set-up for plunge
freezing and freeze substitution I would recommend that procedure over
regular chemical fixation. I can send you the protocol if you want to
consider fs.

Best regards,

Beth Richardson
Botany Dept.
EM Lab Coordinator


} Does anyone have a working protocol for processing aspergillus?
}
} thanks in advance.
}
} rpatel-at-rwja.umdnj.edu






From: lag-at-pegasus.cc.ucf.edu (Lucille A. Giannuzzi)
Date: Fri, 24 May 1996 11:43:36 -0400
Subject: TEM apertures

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Does anyone know where I can purchase TEM apertures (condenser, objective,
and selected area) for a Philips EM301 (besides Philips).

Thanks

**************************************************************************
Lucille A. Giannuzzi, Ph.D. phone: 407 823-5770
University of Central Florida fax: 407 823-0208
Materials Science Program email: lag-at-pegasus.cc.ucf.edu
Dept. of Mechanical and Aerospace Eng.
Orlando, FL 32816-2450
**************************************************************************






From: Susanne Pignolet Brandom :      spb-at-wwa.com
Date: Fri, 24 May 1996 12:12:47 -0500
Subject: Re: Used equipment suppliers

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Message-Id: {2.2.32.19960524171247.006955a0-at-pop.wwa.com}
X-Sender: spb-at-pop.wwa.com
X-Mailer: Windows Eudora Pro Version 2.2 (32)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

A list of companies that broker or directly sell used microscopes and
accessories is available on the WWW at http://www.mwrn.com/product/used.htm

Susanne Pignolet Brandom, Ph.D.
MC Services

MicroWorld Internet Resources at http://www.mwrn.com/
MicroWorld News available by e-mail from MWN-at-mwrn.com

} At 01:39 PM 5/16/96 -0500, you wrote:
} } I am looking for sources of used equipment such as equipment brokers etc.
} } Specifically the types of equipment include coating, microscopy and
} } mechanical test equipment. Secondly I'm also trying to locate sources of
} } discounted consumable supplies for metallurgical polishing work.
} }
} } I would deeply appreciate any leads.
} }
} }
} }
}





From: rnessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Fri, 24 May 1996 08:01:42 -0500 (CDT)
Subject: Re: EDX information

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Message-ID: {199605241808.NAA01386-at-IndyNet.indy.net}
To: "Audette, David" {deaudette-at-corp.olin.com} ,
Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

On Thu, 23 May 1996, Tania Jones wrote:

} Hi there,
}
} On several occasions I have been asked for literature on the how's and
} why's of EDX analysis. Does anyone have any suggestions as to where I
} can obtain such material?
}
} Thanks in advance,
}
} Tania Jones
} Lab Manager
} DynaMotive Technologies

In the recent thread on this topic, Eugene Betrin's book,
"Principles and Practice of X-ray Spectrometric Analysis" was mentioned
(ISBN 0-306-30809-6). I checked out a copy from our Chemistry department
library. It is extremely comprehensive, almost to a fault. Another good
reference source is Joseph Goldstein, etal "Scanning Electron Microscopy
and X-ray Microanalysis" (ISBN 0-306-44175-6). Both of these are texts,
so might be a little deep for the casual reader.

Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu





From: Karen Vaughn :      klv-at-biotech.ufl.edu
Date: Fri, 24 May 1996 12:36:01 GMT
Subject: insect larvae fixation

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A week or so ago I presented a question how to prepare insect eggs for TEM.
I thank everyone who sent us advice in this area, however we are still
unable to successfully infiltrate the eggs. So far this is what has been done.

1) Standard fixation and infiltration protocol.
2) Microwave fixation for impervious biological specimens.
3) Pre-treatment with 1M meta periodate, extended acetone dehydration
followed by infiltration with Spurrs.
All of these methods left us with collapsed samples.

I would like to try a pre-treatment of chitinase and possibly a combination
of chitinase and meta periodate on this sample. Does anyone out there in the
microscopy world have experience with the area or know of a reference?

Once more
Thank you


----------------------------------------------------------------------------
---------
Karen Vaughn Tel.(904) 392-1184
EM Technician
University of Florida Fax.(904) 846-0251
Electron Microscopy Core Laboratory Email. KLV-at-biotech.ufl.edu
Interdisciplinary Center for Biotechnology Research
http://www.biotech.ufl.edu/~emcl/
214 Bartram Hall
Gainesville, Fl 32611







From: Bede Willenbring :      bede.willenbring-at-hbfuller.com
Date: Fri, 24 May 1996 15:34:29 -0500
Subject: Sputter Coater Evaluations - Summary

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As promised, following is the summary of responses I received to my =
request for information on sputter coaters.

There were a total of 21 responses. 17 of those contained evaluations: =
8 Denton Desk II, 3 SPI Sputter Module, 1 Ernest F. Fullam EFFACoater, 1 =
each for Edwards EL2E, Hummer V, Ladd evaporator, and 2 who asked not to =
be included in the summary and have not been.

There were a couple themes that ran through virtually all the responses. =
Almost everyone 1) was happy with what they had, and 2) felt what they =
had was better than what it replaced.

Out of the 18 responses there were two comments about the current =
control response via vacuum control being slow (one each Denton and =
SPI). And one about a slightly mis-shaped glass chamber cylinder which =
required paying a little attention to orientation to insure a good =
vacuum seal (Denton). All other comments were of a positive nature. =
Judging from the total of the comments received, it would appear the =
units available are all reasonably reliable. There were several =
comments about service, all good.

There were 8 responses on film thickness monitors. The "vote" was 5 =
"not necessary" to 3 "necessary". The general feeling seemed to be =
that FTM's were not all that worthwhile primarily because there were too =
many factors affecting their correlation to the metal actually deposited =
on the sample.

I can provide the individual responses (less the two who requiested to =
be excluded) to anyone interested.

My thanks and appreciation to all those who took the time to respond.





From: mcbrande-at-sierra.net (Marc Brande)
Date: Fri, 24 May 1996 09:19:04 -0700
Subject: How to subscribe?

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What is listserv address to subscribe? Thanks

Marc C. Brande, MS, Founder
Cultured Cell Systems Voice: (619) 587-4830
3840 Camino Lindo FAX: (619) 552-1516
San Diego, CA 92122 Email: mcbrande-at-sierra.net






From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Fri, 24 May 1996 20:53:49 +0000
Subject: Re: EDS System Selection (longish)

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X-Sender: (Unverified)
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} Dear Paul,
} In regard to your question, I would like to point out that the Oxford
} Instruments Link ISIS system has a fully quantitative mapping package
} (Quantmap). Full spectrum processing (including digital filtering and
} fitting) followed by a quantitative matrix correction is performed at each
} point. The resulting digital images show elemental concentrations rather
} than just a scaled x-ray intensity map. Standard deviation maps are also
} produced to allow significance testing.
}
} Your local Oxford rep would be delighted to tell you more!
} Best Regards, Julie Sheffield-Parker.
}
} At 05:15 PM 21/05/96 -0700, you wrote:
}
} }
} } Tell me this: who has an EDS system that can obtain a digital image of a
} } sample where a full least-squares fit and ZAF correction has been done on
} } the fly at each pixel, and the resulting image portrays the concentration
} } of the element mapped (not a scaled x-ray intensity map). I've seen papers
} } at national meetings describing this capability, as far back as 5-10 years
} } ago. The limitation for sure isn't the processing speed of the computers
} } now available, or the amount of RAM or hard drive storage space. How hard
} } can it be to do a complete analysis at each pixel in an image?
} }
} } Remember, our goal was to do quantitative EDS.
} }
} } Paul Carpenter
} *************************************************
} From:-
}
} Julie Sheffield-Parker,
} Oxford Instruments Pty. Ltd.,
} P. O. Box 7,
} Pennant Hills,
} NSW 2120,
} Sydney, AUSTRALIA
}
} Tel: ++ 61 2 484 6108
} Fax: ++ 61 2 484 1667
} E-Mail: oisydney-at-ozemail.com.au
}
} *************************************************

Hi,

With respect, I don't think the issue is, for example, the capability to do
quantitative EDX mapping. Surely the question is how? Commercial EDX
systems are just (software) black boxes. Before anybody can have real
confidence, it is at least necessary to know the algorithms used for the
data processing, although the source code would be preferable.

However, having been involved in EM for some time, I would agree with an
earlier comment, that for a large number of microanalytical problems, true
quantitative analysis is not required - knowing that there is ' a lot',
'some', or 'a trace' of a certain element at a specific point is usually
all the information that is required.

Regards
Larry Stoter






From: pyk-at-ornl.gov (Stephen J. Pennycook)
Date: Fri, 24 May 1996 14:13:45 -0500
Subject: Postdoc Positions in Materials Physics

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=46IVE POSTDOCTORAL POSITIONS IN MATERIALS PHYSICS

OAK RIDGE NATIONAL LABORATORY, SOLID STATE DIVISION

=46ive postdoctoral openings are available in the Electron Microscopy Group,
ORNL, in a joint experimental and theoretical program coupling atomic scale
imaging of interfaces with state of the art computational studies on
interface structure and properties. The Solid State Division has one of
the world's finest facilities for atomic scale imaging of materials: a VG
Microscopes HB603 300 kV scanning transmission electron microscope with a
1.26=C5 probe size provides direct, Z-contrast imaging capabilities for
interfaces in materials. A VG Microscopes HB501UX 100 kV microscope with a
high sensitivity parallel EELS capability provides atomic resolution
spectroscopy. The Electron Microscopy Group has a Silicon Graphics
PowerIndigo workstation with a Molecular Simulations' Cerius 2 package
incorporating the CASTEP pseudopotential code. The Division has a number
of additional workstations, and access to extensive parallel computing
capabilities, the Intel Paragon XP/S 35 and XP/S 150 with 512 and 2048
processors respectively.

In addition, postdoctoral positions are available in collaboration with the
Ion-Solid Interactions Group, ORNL, Northwestern University and the
University of Illinois at Chicago:

(I) Grain Boundary Structure and Properties:

Two positions are available in the Solid State Division, ORNL (Dr. Steve
Pennycook and Dr. Richard Wood). Atomic scale imaging and spectroscopy,
macroscopic property measurements and theoretical simulations will unravel
the link between grain boundary atomic structure and the electrical
transport properties of electronic ceramics and superconductors. The
program will focus on bicrystals and thin films, and investigate the
applicability of the structural unit model for predicting properties, its
extension to three dimensions, and impurity site imaging and spectroscopy
with a view to grain boundary engineering. Theoretical studies will range
from empirical bond-valence sums to full ab initio calculations. Two
positions are available, either one theorist and one experimentalist, or
alternatively, qualified candidates may wish to perform theory and
experiment on a specific class of materials.

(II) Interfaces in Functional Magnetic/Electroceramic Materials:

This position is a collaborative venture between the Solid State Division
(Dr. Steve Pennycook) and The Department of Materials Science &
Engineering, Northwestern University (Prof. Vinayak P. Dravid), focussing
on the role of interfaces in functional magnetic and electroceramic
materials, e.g. colossal magnetoresistant (CMR) materials, PTCR, GBBLC
oxides. Atomic resolution Z-contrast imaging and EELS will be used to
determine the atomic and electronic structure of interfaces in thin film
multilayers and bulk polycrystals for correlation with macroscopic
properties. The successful applicant will reside at ORNL for at least 2/3
of time, and serve as a liaison between Northwestern University and ORNL.
Instrumentation available at NU in the newly restructured Electron Probe
Instrumentation Center (EPIC) includes a Hitachi HF-2000 FEG TEM/STEM with
x-ray, EELS, in-situ I/V cryogenic holder and e- holography capability, and
a Hitachi S4500-II FEG SEM with x-ray, EBSP/OIM and liquid helium stage
with in-situ I/V probes. This position also involves collaborative work on
other interface controlled materials such as electroceramics and high Tc
compounds.

(III) Structure-Property Relationships in MBE grown Optoelectronic Devices:

This position is a collaborative venture between the Solid State Division
(Dr. Steve Pennycook) and the Department of Physics, University of Illinois
Chicago (UIC, Prof. Nigel Browning), to investigate the fundamental link
between the properties of MBE grown optoelectronic devices and the atomic
structure of the film-substrate interface, leading ultimately to the
production of compliant and alternative substrates for coherent
heteroepitaxy. Z-contrast imaging and electron energy loss spectroscopy
offer unique insights into the nature of the compliant interface, and film
and defect nucleation mechanisms. The Microphysics Laboratory at UIC is
internationally recognized for its extensive facilities and expertise in
MBE growth, including an OPUS 45 MBE facility for the transfer and
processing of 5-inch wafers, and two RIBER 2300 MBE systems with extensive
growth monitoring and in-situ surface analysis capabilities. This
position offers a unique opportunity to work at a fundamental level in an
area of high technological significance. Applicants should demonstrate
extensive knowledge of crystal defects.

(IV) Ion-Solid Interactions:

Research in the Ion-Solid Interations Group is materials oriented and
involves ion beam synthesis or modification of materials to produce novel
and technologically relevant properties. Research in which the successful
candidate will be involved includes (a) the formation of nanocrystals and
quantum dots in insulators to produce unique optoelectronic responses, (b)
synthesis of buried layers in semiconductors for interconnect or isolation
applications, and (c) the physics of ion-induced defects in semiconductors.
The diverse scope of this program and the interactions it has fomented
with other national laboratories, industry, and universities will provide
the successful candidate a unique opportunity for professional growth.
Applicants should have extensive experience in transmission electron
microscopy and the preparation of cross section specimens.


Successful candidates will be recent Ph.D. graduates in physics,
metallurgy, or materials science with a sound background in the relevent
materials issues and a burning ambition to develop a forefront area in
materials physics. If this is you, send your resume and publication list
to Dr. S. J. Pennycook at the address below. Prior experience using
transmission electron microscopy is essential only where explicitly
stated; consideration will be based on the candidates overall potential for
success in the field. Positions are for one year initially, normally
renewed for a second year and possibly a third. ORNL is a multipurpose
national laboratory managed by Lockheed Martin Energy Research Corporation
for the U.S. Department of Energy. ORNL is an equal opportunity employer
committed to building and maintaining a diverse work force.


----------------------------------------------------------------------------
------------------------------------------Stephen J. Pennycook
Oak Ridge National Laboratory
PO Box 2008
Oak Ridge TN 37831-6030

phone: (615) 574-5504
fax: (615) 574-4143
----------------------------------------------------------------------------
------------------------------------------






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Fri, 24 May 1996 00:30:28, -0500
Subject: Retirement announcement

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Message-Id: {199605240430.AAA05652-at-mime2.prodigy.com}
X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc06

-- [ From: Charles A. Garber., Ph.d * EMC.Ver #2.10P ] --

Forgive me if this is thought to not be an appropriate use of the
listserver, but I just found out today that Dr. Wilf Gee, VG Microtech
UK (Fisons Instruments) is retiring the end of the month.

Dr. Gee for many years was the chief engineer (and he held other high
positions as well) at what was once Polaron Equipment Ltd, and which
later was acquired by Bio-Rad. That business was sold to Fisons in
about 1992.

It is my understanding that Dr. Gee might very well be the only person
who really knows the inner workings of some of the larger equipment
items, especially that which was made some years ago by Polaron
Equipment. Certainly more than a few persons including yours truly
have grown to depend on Wilf's advice and guidance when it came to
keeping in operation equipment made some years ago.

Wilf was willing to have me post this information as well as his e-mail
address: {wgee-at-vacgen.fisons.co.uk} . Of course, the e-mail address
is probably going to "expire" at the end of the month as well, so
anyone wanting to say in touch with him should send him their address
before that time. Wilf, always wanting to help someone in need with
their equipment, I am sure, even in his retirement, would want to pass
on what he knows.

At least I for one wish Wilf a happy and healthy coming retirement!

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.com

####################################
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####################################
======================================================




From: rozeveld-at-egr.msu.edu (Steve Rozeveld) (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Sat, 25 May 1996 09:30:59 -0500
Subject: job opening

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A research associate is sought to establish, operate, and maintain the
Environmental Scanning Electron Microscope which is to be set up as a
University user and outreach facility. The individual is expected to conduct
research on materials characterization and behavior with the ESEM in
collaboration with faculty, students and government personnel. Research
areas include composite materials, metals, ceramics, polymers, fiber
reinforced cement, asphalts, food, packaging materials, soils, plants and
wood products. The ESEM is equipped with various stages for in situ
stress-temperature-environment studies over a wide range of conditions. The
ESEM is also equipped with an EDS detector for chemical analysis.

The person occupying this position will be responsible for operation and
maintenance of the ESEM as well as the training of faculty, staff, graduate
students and non-campus users. In addition, the successful candidate will
conduct research with the ESEM and is expected to develop contacts with
potential off-campus users in the local and state-wide community to assist
in generating funds for the support and maintenance of the ESEM. The
responsibilities for this position are divided approximately as follows:
Research-50%, Outreach-25%, Administration-15%, Teaching-10%.

Qualifications: A Ph.D. in Materials Science, Physics, Chemistry, Geology,
Biology or Engineering is required. A combination of course work and
hands-on research experience with SEM is required, ESEM is preferred. The
applicant must exhibit very high levels of oral and written communication
skills. Previous experience in budgeting and accounting would be useful.

Position is open until filled. Salary is commensurate with qualifications
and experience. Send a complete curriculum vitae, graduate level transcript,
and three references to:
Professor Lawrence T. Drzal,
Michigan State University, Composite Materials and Structures Center,
Engineering Research Complex B-100,
East Lansing, MI 48824-1326 tel: 517/ 353-5466 fax: 517/ 432-1634

Contact Michael Rich, Research Specialist and Laboratory Manager, for
further information regarding this position. RICH-at-EGR.MSU.EDU. Michigan
State University is an equal-opportunity institution.








From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Sat, 25 May 1996 10:42:52, -0500
Subject: Service contracts

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X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc06

-- [ From: Charles A. Garber., Ph.d * EMC.Ver #2.10P ] --

The concept of CIC, which I had not heard of previously, was described
as follows:
====================================
"CIC Agency is a Risk Management Corporation .....essentially an
insurance company."
====================================

This discussion is beginning to sound somewhat like our national debate
on health care and the HMO approach in that people are a) complaining
about the ever increasingly higher costs of providing microscope care,
b) have a perception that the people offering such contracts for this
care are taking advantage of what is thought to be a captive market,
and c) are under no financial incentives (e.g. the patient microscope
lab managers) to help reduce the costs for the provision of the
services on the part of the service provider.

I can remember some years ago having this very samediscussion with the
National Service Manager of one of the major column instrument
manufacturers in the USA. He said "legally" it could never happen
because of "GSA Contract considerations" as well as other reasons. I
presume he was talking about it from the standpoint of their particular
firm offering service contracts at different prices to different people,
since the pricing would be "experience based". There is of course a
concept that one can not sell something at a lower cost than they would
sell the same thing for to Uncle Sam. On the other hand, maybe he
just did not understand what I was suggesting. He might even have been
incorrect in his legal interpretation and whether the GSA concern would
apply in this kind of a situation.

He went on to say "the approach you are suggesting is already in place,
our people when they visit a customer spend part of their time looking
for the embryo of tomorrow's problem on today's visit because it in our
own self-interest to keep service calls to a minimum". Well, that
could certainly be correct, but as has been found to be the case for
the provision of medical services, there are many aspects of one's
health that just are not apparent during that brief visit and exam. And
the patient has to take ultimate responsibility for watching for signs
of anything not being right. Yet at the same time, because of human
nature being what it is, the existence of a "co-pay" is a vital
component of any kind of cost containment process.

And in today's environment, service engineers seem to be so harried,
and so pressed to increase the number of service calls made per unit of
time, that I am not sure just how much discretionary time is really
left any more to seek out those "problems just waiting to happen".


So unless firms like CIC are going onto the premises of their client
firms, holding training sessions and seminars on how one could operate
their laboratory from the perspective of reducing the costs to the
insurer, so to speak, then CIC is indeed acting only as an insurer, and
is NOT acting as a provider of a broad risk management program for the
laboratory (one that has as the goal to help that laboratory client to
operate in a way that the costs to CIC would be less rather than more,
the incentive being a lower premium in coming years). And only a
believer in the tooth fairy would also believe that somehow, when it
was all said and done, after taking into consideration this extra layer
of bureaucracy, not to mention corporate profit, that they were going
to be getting the exact same service for less money.

And this is because just like with an HMO, there are only a limited
number of ways the "insurer" can reduce costs:

a) limit or ration benefits (for example, agree to perform only a fixed
number of "emergency" calls in one year) under the Agreement. Maybe
routine service calls would be scheduled more at the convenience of the
service provider rather than the convenience of the customer. There
could be other accommodations that might mean a lot to the insurer but
not represent much of a "give back" to the laboratory.

b) institute a meaningful co-pay, so that each time a service engineer
is called in to work his charm, there is a definite "out of pocket"
cost paid, thereby limiting (just as it is meant to do for visits to a
physician's office) the total number of visits. In our own laboratory
situation, it is just amazing how much that extra incentive to solve
problems on the phone instead of calling for an emergency visit can
reduce the actual number of required visits.

c) institute a real program of loss prevention and risk analysis,
where by on a regular basis, the laboratory is visited, and "hazardous"
(with regard to future equipment failures) situations are spotted and
remedied before causing the insurance company an expensive "loss". One
does not have to eliminate too many emergency field service calls
before such a program would provide large economic rewards. Although I
have no hard data, but just the experience of managing column
instruments for nearly thirty years, just spotting a water chiller
about to konk out or a pump belt getting ready to break could save a
great deal of down time and money.

We ourselves made a comparison between the frequency of needed calls
for service engineers before vs. after I dropped all annual service
contracts in our own laboratories. It was amazing how many fewer
visits we seem to be able to get along with, without any noticeable
increase in down time. One might think that as instruments get older,
then they would require more service, but for us, that has not been the
case. Perhaps it is the pedigree of our instruments. The several
major disasters we have had, although expensive, still leave us light
years ahead of where we would have been had we been covering the
instruments under paid service contracts (at one time six) all these
years. And the "disasters" and ensuing down time would have happened
anyhow, service contract or no service contract. But those are the
outliers of experience that do occasionally occur and unfortunately too
often become the horror stories on which the sales of service contracts
are promoted.

If some agency was going to establish some kind of insurance company
that at the same time, would institute a good risk management approach
to the servicing of column instruments, EDS systems, etc., then I would
sure like to know about it myself. We would be among the first to want
to sign up. We would see that as a really worth while program since it
would have a real chance of reducing the costs to maintain a column
instrument.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.com

####################################
WWW: http://www.2spi.com
####################################
======================================================




From: A. Greene :      ablue-at-mail.io.com
Date: Sat, 25 May 1996 10:46:11 -0500 (CDT)
Subject: Chilled Water

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Hello, I was away for a few days and noticed much discussion about cooling
water. If I may, at this late date, I would like to spalsh around a bit
myself.

Many years ago, I was a Service Engineer for Philips and had about 15
Haskris systems running in Florida. My philosophy has always been
Simplicity in concept and exicution or the well known "KISS Principal."
Anyway, the formula for sucess was to use dionized water or distilled
water, put three or four drops of oil on top of the water, in the reservour
and if you have some, toss a couple crystals of Iodine in the tank. A five
micron water filter is a good idea, also. The way it works, as I understand
it...Since water is such a wonderful solvent, it leaches away a small bit of
the material through which it travels and creates a eguilibrium. The water
sort of tunes itself to the system. A fungicide or other poison does what
it should but what is the result? My experience has been the creation of
gray slime in the system. A monolayer of oil on the reservour keeps algae
away. Additives have been the cause of many problems with seals, small
openings where the water is supposed to flow and pump failures.

Thanks for your time.

Alex Greene
Scientific Instrumentation Services, Inc.
Austin, Texas





From: A. Greene :      ablue-at-mail.io.com
Date: Sat, 25 May 1996 12:49:31 -0500 (CDT)
Subject: Re: TEM Screen Coating

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Hi Phil, I always wondered how to coat a screen. Thanks very much for the
detailed information. For anyone not up to trying it themselves, Grant
Scientific does a great job of screen recoating. They are located in
Gilbert, South Carolina and their phone no. is 803/892-2841.

Alex Greene
Scientific Instrumentation Services, Inc.
Austin, Texas


At 11:23 AM 5/22/96 -0400, you wrote:
} To all interested:
}
} As I said I used green phosphor from JEOL, cat. # 423-011. Call and see
} if this number still holds true.
}
} You will need a collodion solution: 4 grams parlodian
} 25 ml absolute ethel alcohol
} 75 ml ether
}
} You will also need a dish big enough for the screen and a cover
} (preferably glass) with a small hole for removal of the liquid.
}
}
} 1. Remove the old coating from the screen by washing in acetone. The
} cleaned plate must be free from all particles of matter and the surface
} must be free from blemishes and scratches.
}
} 2. Prepare a sufficient volume of 4% (w/v) suspension of phosphor powder
} in acetone containing about 1% collodion. The total volume must be
} sufficent to fill selected dish with liquid to a depth of about 1cm above
} the surface of the screen in position on the bottom of the dish.
}
} 3. Agitate the suspension vigorously (I used sonicator) then pour it
} rapidly into the dish.
}
} 4. Wait about 5-10 seconds to allow larger particles to settle and
} swirling to cease.
}
} 5. Slide the plate smoothly into the liquid, preferably without scraping
} the bottom of the dish.
}
} 6. Cover the dish and leave to settle. When the suspension has settled
} and the remaining liquid is clear, draw off the liquid by inserting a
} suction tube through the previously prepared hole in the lid of the
} dish. It is extremely important not ot disturb the screen plate or the
} liquid above it in any way as this is done. Draw off the liquid steadily
} and slowly then remove the suction tube.
}
} 7. Leave the screen to dry without any disturbance of any kind. Do not
} lift the lid to inspect the screen until the powder is quite dry because
} a slight change in drying conditions can produce a visible mark on the
} damp surface.
}
} 8. When the powder is quite dry, remove the screen and wipe off any
} excess phosphor from the back and sides of the screen.
}
} 9. Install.
}
} 10. A newly coated screen will outgas for a short time when it is first
} placed in the microscope. Pumping times may therefore be a little longer
} than normal at first.
}
} A very small particle size is desirable for high resolution screens and
} it may be advantageous to agitate the the phosphor suspension in an
} ultrasonicator before putting it into the dish.
}
} Hope this helps. It can get tricky and you may not get it the first time.
} Patience really helps!
}
} Peace,
}
} Phil Rutledge
}
}





From: zaluzec-at-sparc5.microscopy.com (Nestor J. Zaluzec)
Date: Sat, 25 May 1996 21:54:25 -0500
Subject: Microscopy & Microanalysis -96 Program

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Message-Id: {199605260250.VAA01233-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


The general (daily) program for Microscopy & Microanalysis - 96
is now available on-line at the following URL.

http://www.msa.microscopy.com

This listing outlines the , daily schedule of symposia,
poster sessions, platform sessions, business meetings, workshops
and tutorials. A listing of authors and abstract titles will be available
shortly at the same site, and will be announced when on-line.

Nestor
Your Friendly Neighborhood SysOp







From: John Gabrovsek :      gabrovj-at-cesmtp.ccf.org
Date: Sun, 26 May 1996 11:12:38 -0400
Subject: TEM em-markers

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Message-Id: {s1a83d69.043-at-cesmtp.ccf.org}
X-Mailer: Novell GroupWise 4.1

Hi everybody,
I have a question. One researcher is interested in passage of plastic
microspheres about 100 nm mean diameter through the arterial wall of
the experimental animal. He would like to know the path and where the
microspheres are accumulating. I was asked for advice how to prepare
the samples that the microspheres would be visible in TEM
preparation.Plastic microspheres are translucent for electron beam
and therefore invisible in TEM preparation. My question.Is there any
possibility to attach an EM marker to these microspheres?
Any suggestion will be appreciated.
TIA
John Gabrovsek
CCF Cleveland, Ohio





From: Probing & Structure :      pns-at-ultra.net.au
Date: Mon, 27 May 1996 08:31:49 +1000
Subject: Re: insect larvae fixation (longish)

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Karen: Your problem is not new. About 30 years ago I was put in charge of an
small EM lab. One of the problems which was to challenge my enthusiasm,
which was rather moderated by a lack of technical acumen, was a project on
the development of the cricket egg. Its leathery case withstood all
ingenuity. I think that the only kind of "success" we had was with warm 2%
KMnO4. This only fixes membranes and is now a fixative of ill repute.

Perhaps we should have tried 2% OsO4 or just vapour at perhaps 40 degrees C.
(Take care of fumes!) Perhaps a penetrating agent like the very toxic DMSO
could have helped - but I wonder: would it change structure before fixation
is effective.

Glutaraldehyde is too large a molecule, although it penetrates further than
Os; a tight case like the cricket's egg is more likely to be penetrated by a
large atom like Os, than a large molecule. In my experience, difficult
tissues like these, when surrounded GA, are more likely to go into
autolysis. Fixation may only occur when the "post fixative" is added.
Certainly good fixation is required prior to any plastic infiltration; it's
too easy to blame infiltration when poor fixation is the more likely
underlying problem.

If I had to deal with those cricket eggs now, I would look at more recently
developed alternative techniques. Maybe freeze etching or perhaps freeze
substitution, but I expect that some cryo fixation /sectioning techniques
really holds the greatest promise.

If all of this seems difficult, try dry aleurone. That is the live, outer
layer of the wheat grain. Its easy, after it is imbibed, but try it dry.
"Old fashioned TEM" still has challenges greater than printing overheads and
computer imaging!

Jim Darley

At 12:36 24-05-96 GMT, you wrote:
} A week or so ago I presented a question how to prepare insect eggs for TEM.
} I thank everyone who sent us advice in this area, however we are still
} unable to successfully infiltrate the eggs. So far this is what has been done.
}
} 1) Standard fixation and infiltration protocol.
} 2) Microwave fixation for impervious biological specimens.
} 3) Pre-treatment with 1M meta periodate, extended acetone dehydration
} followed by infiltration with Spurrs.
} All of these methods left us with collapsed samples.
}
} I would like to try a pre-treatment of chitinase and possibly a combination
} of chitinase and meta periodate on this sample. Does anyone out there in the
} microscopy world have experience with the area or know of a reference?
}
} Once more
} Thank you
}
}
} ----------------------------------------------------------------------------
} ---------
} Karen Vaughn Tel.(904) 392-1184
} EM Technician
} University of Florida Fax.(904) 846-0251
} Electron Microscopy Core Laboratory Email. KLV-at-biotech.ufl.edu
} Interdisciplinary Center for Biotechnology Research
} http://www.biotech.ufl.edu/~emcl/
} 214 Bartram Hall
} Gainesville, Fl 32611
}
}
}
}
}
Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
Internet Catalogue: http://www.ultra.net/~pns/





From: rgwhite-at-vaxc.cc.monash.edu.au (Rosemary White)
Date: Mon, 27 May 1996 17:19:44 +1200
Subject: Re: insect larvae fixation (longish)

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Re:

} Perhaps we should have tried 2% OsO4 or just vapour at perhaps 40 degrees C.
} (Take care of fumes!) Perhaps a penetrating agent like the very toxic DMSO
} could have helped - but I wonder: would it change structure before fixation
} is effective.

I have no experience with insect larvae at all, but difficult plant
material may have similar problems. Incubations with high concentrations -
10% - of DMSO in the fixative preserve plant ultrastructure very well - at
least for immunofluorescence. Not sure how the material would look under
the EM.

Colleagues here deal with desiccated plant material - will get back re. how
they prepare material for TEM.

extra good luck,


Rosemary White
__ /
Department of Ecology _/ \__/ \
and Evolutionary Biology / \
Monash University / Australia \
Clayton, Victoria 3168 \ ____ /
phone 61-3-9905 5670 \_/ \_*_/
fax 61-3-9905 5613 __
email rgwhite-at-sci.monash.edu.au \/
or rgwhite-at-vaxc.cc.monash.edu.au






From: A. Kent Christensen :      akc-at-umich.edu
Date: Mon, 27 May 1996 10:20:35 -0400 (EDT)
Subject: Re: insect larvae fixation (longish)

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Karen,

Another fixative that may be worth trying would be acrolein ("tear gas"),
which has a reputation for rapid penetration. These references may be of
interest:


Luft JH, 1959. The use of acrolein as a fixative for light and electron
microscopy. Anat Rec 133:305.

King JC, Lechan RM, Kugel G, Anthony ELP, 1983. AcroleIn: A fixative for
immunocytochemical localization of peptides in the central nervous system.
J Histochem Cytochem 31:62-68.

Grote M, Dolecek C, Vanree R, Valenta R. 1994. Immunogold electron
microscopic localization of timothy grass (_Phleum pratense_) pollen major
allergens PHL P I and PHL P V after anhydrous fixation in acrolein vapor.
J Histochem Cytochem 42 (3 C):427-43 1.

A. Kent Christensen
University of Michigan
{akc-at-umich.edu}

-------------------------------------

On Mon, 27 May 1996, Probing & Structure wrote:

} Karen: Your problem is not new. About 30 years ago I was put in charge of an
} small EM lab. One of the problems which was to challenge my enthusiasm,
} which was rather moderated by a lack of technical acumen, was a project on
} the development of the cricket egg. Its leathery case withstood all
} ingenuity. I think that the only kind of "success" we had was with warm 2%
} KMnO4. This only fixes membranes and is now a fixative of ill repute.
}
} Perhaps we should have tried 2% OsO4 or just vapour at perhaps 40 degrees C.
} (Take care of fumes!) Perhaps a penetrating agent like the very toxic DMSO
} could have helped - but I wonder: would it change structure before fixation
} is effective.
}
} Glutaraldehyde is too large a molecule, although it penetrates further than
} Os; a tight case like the cricket's egg is more likely to be penetrated by a
} large atom like Os, than a large molecule. In my experience, difficult
} tissues like these, when surrounded GA, are more likely to go into
} autolysis. Fixation may only occur when the "post fixative" is added.
} Certainly good fixation is required prior to any plastic infiltration; it's
} too easy to blame infiltration when poor fixation is the more likely
} underlying problem.
}
} If I had to deal with those cricket eggs now, I would look at more recently
} developed alternative techniques. Maybe freeze etching or perhaps freeze
} substitution, but I expect that some cryo fixation /sectioning techniques
} really holds the greatest promise.
}
} If all of this seems difficult, try dry aleurone. That is the live, outer
} layer of the wheat grain. Its easy, after it is imbibed, but try it dry.
} "Old fashioned TEM" still has challenges greater than printing overheads and
} computer imaging!
}
} Jim Darley
}
} At 12:36 24-05-96 GMT, you wrote:
} } A week or so ago I presented a question how to prepare insect eggs for TEM.
} } I thank everyone who sent us advice in this area, however we are still
} } unable to successfully infiltrate the eggs. So far this is what has been done.
} }
} } 1) Standard fixation and infiltration protocol.
} } 2) Microwave fixation for impervious biological specimens.
} } 3) Pre-treatment with 1M meta periodate, extended acetone dehydration
} } followed by infiltration with Spurrs.
} } All of these methods left us with collapsed samples.
} }
} } I would like to try a pre-treatment of chitinase and possibly a combination
} } of chitinase and meta periodate on this sample. Does anyone out there in the
} } microscopy world have experience with the area or know of a reference?
} }
} } Once more
} } Thank you
} }
} }
} } ----------------------------------------------------------------------------
} } ---------
} } Karen Vaughn Tel.(904) 392-1184
} } EM Technician
} } University of Florida Fax.(904) 846-0251
} } Electron Microscopy Core Laboratory Email. KLV-at-biotech.ufl.edu
} } Interdisciplinary Center for Biotechnology Research
} } http://www.biotech.ufl.edu/~emcl/
} } 214 Bartram Hall
} } Gainesville, Fl 32611
} }
} }
} }
} }
} }
} Probing & Structure
} Microscopy Supplies & Accessories
}
} Phone +61 77 740 370 Fax: +61 77 892 313
} Internet Catalogue: http://www.ultra.net/~pns/
}
}




From: Ming Chen :      mingchen-at-gpu.srv.ualberta.ca
Date: Mon, 27 May 1996 09:34:58 -0600 (MDT)
Subject: Re: TEM em-markers

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Do you fix your specimens in osmium tetraoxide ? If your answer is Yes,
then it will have a better chance to locate the plastic microspheres in
the arterial wall without any addition EM marker.


On Sun, 26 May 1996,
John Gabrovsek wrote:

} Hi everybody,
} I have a question. One researcher is interested in passage of plastic
} microspheres about 100 nm mean diameter through the arterial wall of
} the experimental animal. He would like to know the path and where the
} microspheres are accumulating. I was asked for advice how to prepare
} the samples that the microspheres would be visible in TEM
} preparation.Plastic microspheres are translucent for electron beam
} and therefore invisible in TEM preparation. My question.Is there any
} possibility to attach an EM marker to these microspheres?
} Any suggestion will be appreciated.
} TIA
} John Gabrovsek
} CCF Cleveland, Ohio
}
}


 ***********************************************
 * Ming H. Chen, PhD *
 * Medicine/Dentistry Electron Microscopy Unit *
 * mingchen-at-gpu.srv.ualberta.ca *
 ***********************************************








From: shayashi-at-opt.olympus.co.jp (Shinichi Hayashi)
Date: Tue, 28 May 1996 17:49:49 +0900
Subject: Photochromic pigment

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-- [ From: Paul E. Fischione * EMC.Ver #2.3 ] --

I would like to share some experience dimpling both Ti/SiC and Al/SiC.
Both of these materials require mechanical thinning to less than 5 microns
in order to minimize the difficulties associated with dissimilar ion
milling rates between the SiC fibers and the bulk material. Aspects that
need to be considered, particularly in the case of Al/SiC is that the bulk
material will smear rather than grind, and the bond between the SiC fibers
and the bulk material is usually weak. I have found that a minimal force
applied to the specimen results in the SiC dropping out of the matrix and
becoming suspended in the abrasive media. When this occurs, significant
scratching of the specimen's surface will result.

After a great deal of experimentation, I was able to successfully dimple
both Ti/SiC and Al/SiC. The dimpling grinder used in the research was our
Model 2000 Specimen Prep System. The conditions initially utilized were a
force of approximately 20 grams, a grinding rate of 1.0 microns/minute and
a 3 micron diamond abrasive. Dimpling was conducted to a thickness of 15
microns when the rate was reduced to 0.5 microns/min and the abrasive was
changed to 1 micron diamond. This was done until the sample thickness was
10 microns. The grinding rate was then reduced to 0.2 microns/minute, the
force to 15 grams and the abrasive to 0.25 micron diamond. Dimpling was
terminated at a specimen thickness of 3 microns. During the final few
microns of specimen material removal the abrasive was changed every 2-3
minutes.

The key to the success in dimpling was having the ability to program a
grinding rate. By establishing a grinding rate less than the material's
actual removal rate (as determined by the applied force and abrasive grit
size), incremental amounts of material can be readily removed. The rate
control mechanism exhibits a resolution of 37 nm, and because the grinding
wheel stage is supported by the rate control stage, the grinding wheel
actually becomes more concentric with use. It is important to note that
wheel vibration due to an eccentric wheel is the single most contributing
factor to specimen breakage.

For a more complete description please refer to my paper in MRS 199.

I hope this helps.

Paul

paul.fischione-at-internetmci.com
Paul E. Fischione, President
E.A. Fischione Instruments, Inc.
9003 Corporate Circle
Export, PA 15632 USA
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------- FORWARD, Original message follows -------


Dear lists:

My colleague would like to pose a question into this cyber space:

} Hello all,
}
} Does anyone know the spectroscopic properties(peak emission wavelength and
} FWHM, etc.) of the photochromic pigment such as below?
}
} 1) Name of the photochromic pigment:
} 1,3,3-trimethylindolime-6-nitrobenzospiropyran, generally called TNSB for
} short.
} 2) Circumstance:
} This photochromic pigment is in the Silicon oil.
} 3) Excitation source:
} The excitation source is the Nitrogen Laser(wavelength is 337.1nm).
}
} Many thanks in advance.
} ------------------------------

Please reply directly to me. Thank you.
--------------------------------
Shinichi Hayashi
Optical R&D 2nd Group
Olympus Optical Co., Ltd.
Fax: +81 426 42 2102
e-mail: shayashi-at-opt.olympus.co.jp





From: dshubito-at-d.imap.itd.umich.edu (Dennis Shubitowski)
Date: Tue, 28 May 1996 09:01:18 -0400
Subject: Re: EDX information

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Message-Id: {v02140b01add0a8147b5f-at-[141.211.157.61]}
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An excellent, short handbook about EDX can be found in the Royal
microscopical Society series #05: "X-ray Microanalysis in Electron
Microscopy for Biologists" by A.J. Morgan. It is about 70 pages
long and glosses over introductions and terms, x-ray production,
x-ray detection, qualitative analysis, quantitative analysis, and
specimen preparation.

Oxford University Press
ISBN 0-19-856409-0

Dennis Shubitowski
University of Michigan
School of Dentistry
dshubito-at-umich.edu

Dennis Shubitowski
University of Michigan
School of Dentistry
dshubito-at-umich.edu






From: slakmon-at-scottscientific.com (P. Slakmon)
Date: Tue, 28 May 1996 09:34:19 -0400 (EDT)
Subject: LKB Ultrotome Nova

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If anyone has experience problems with the light fixture located on the head
of an LKB Ultrotome Nova and has fixed it or found the problem, please send
me your feedback, it would be greatly appreciated.

Thank You,

Philip
_______________________________________________
SCOTT SCIENTIFIC
P.O. Box 66552 Station Cavendish
Montreal, Quebec, H4W 3J6, Canada

Telephone: 514-485-2309 Fax: 514-485-9931
Voice Mail: 514-888-6509

WWW Site: http://www.scottscientific.com

E-Mail: info-at-scottscientific.com
sales-at-scottscientific.com
admin-at-scottscientific.com
links-at-scottscientific.com
_______________________________________________





From: pdf-at-fullam.com
Date: Tue, 28 May 1996 10:47:57 -0400 (EDT)
Subject: Re: TEM apertures

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We have made apertures for many years. More information on our apertures is
available on the WWW at http://www.fullam.com/aperture.htm or you can
contact us at the numbers listed in our signature.

Dianne Fullam


} Does anyone know where I can purchase TEM apertures (condenser, objective,
} and selected area) for a Philips EM301 (besides Philips).
}
} Thanks
}
} **************************************************************************
} Lucille A. Giannuzzi, Ph.D. phone: 407 823-5770
} University of Central Florida fax: 407 823-0208
} Materials Science Program email: lag-at-pegasus.cc.ucf.edu
} Dept. of Mechanical and Aerospace Eng.
} Orlando, FL 32816-2450
} **************************************************************************
}
}
}
Ernest F. Fullam, Inc.
Phone: (518) 785-5533 FAX: (518) 785-8647
E-Mail: pdf-at-fullam.com

************************************************************
* Complete on-line product listing: http://www.fullam.com/ *
************************************************************





From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Tue, 28 May 1996 09:20:30 GMT
Subject: Re: TEM em-markers

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} To: John Gabrovsek {gabrovj-at-cesmtp.ccf.org}
} From: gwe-at-biotech.ufl.edu (Greg Erdos)
} Subject: Re: TEM em-markers
} Cc:
} Bcc:
} X-Attachments:
}
} } Hi everybody,
} } I have a question. One researcher is interested in passage of plastic
} } microspheres about 100 nm................
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
} Your researcher might consider magnetic microspheres, since they are loaded
with iron and are quite visible at the TEM. I have embedded and section
them using routine techniques. Also most of these plastic microsperes have
reactive surfaces so you might be able to attach a protein that has been
coupled to small colloidal gold
}
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: JCrandall-at-shriver.org (James Crandall)
Date: Tue, 28 May 1996 13:20:28 -0500
Subject: New subscriber

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Sorry to bother, but could someone please let me know how a new person
would sign on to this list server? It's been so long that I have been on
that I have forgotten and my recent hard disk crash wiped out the old
messages for instructions.

TIA

Jim

*****************************************
Jim Crandall, Ph.D.
Senior Neurobiologist
Department of Developmental Neurobiology
E. Kennedy Shriver Center
200 Trapelo Road
Waltham, MA 02154
USA

tel 617-642-0278
fax 617-893-4018
email jcrandall-at-shriver.org
*****************************************






From: Debbie Cassout :      DCASSOUT-at-TVMDL.TAMU.EDU
Date: Tue, 28 May 1996 12:04:57 -0500
Subject: TEM apertures -Reply

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Message-Id: {s1aaec3a.031-at-TVMDL.TAMU.EDU}
X-Mailer: Novell GroupWise 4.1

I've always ordered apertures (platinum and gold foil) for my Philips 301
through Electron Microscopy Sciences. Their phone # is
1-800-523-5874. I've been happy with their products and service, and
their prices are very competitive.

Debbie Cassout
E.M. Dept.
Texas Veterinary Medical Diagnostic Lab.
College Station, TX

} } } Lucille A. Giannuzzi {lag-at-pegasus.cc.ucf.edu} 05/24/96 10:43am
} } }
Does anyone know where I can purchase TEM apertures (condenser,
objective, and selected area) for a Philips EM301 (besides Philips).

Thanks

**************************************************************************
Lucille A. Giannuzzi, Ph.D. phone: 407 823-5770
University of Central Florida fax: 407 823-0208
Materials Science Program email: lag-at-pegasus.cc.ucf.edu
Dept. of Mechanical and Aerospace Eng.
Orlando, FL 32816-2450
**************************************************************************








From: Hand-at-nso1.uchc.edu (Hand,Arthur)
Date: Tue, 28 May 1996 15:41:28 -0400
Subject: Feulgen stain of LR Gold/White sections

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Greetings: Has anyone had experience with Feulgen staining of LR Gold or LR
White sections? We would like to use this procedure to verify fertilization
of frog oocytes. Methods, tips, pitfalls, etc. would be appreciated.

Thanks in advance,

Arthur R. Hand
Central EM Facility
UConn Health Center
Farmington, CT





From: Robert Yancy :      Robert_Yancy-at-quickmail.cicagency.com
Date: 27 May 1996 15:02:19 -0500
Subject: CIC Services

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My interest in the CIC Services took me to their home page where I
could e-mail them for more information. I think the idea for this
type of "insurance company" pooling of service contracts makes sense.
In fact that is exactly what each individual service contract really
amounts to... insurance to repair future problems. Their response to
my e-mail is attached for anyone interested. Look into it or not as
you wish.
Linda Fox lfox1-at-wpo.it.luc.edu
Received: from quickmail.cicagency.com ([206.104.48.12])
by mail.cicagency.com (Netscape Mail Server v1.1) with SMTP
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Message-ID: {n1378913100.90616-at-quickmail.cicagency.com}

Subject: Time: 2:44 PM
CIC Services Date: 5/27/96

Thank you for the notification of our services being discussed on your list.
You are exactly right in your assessment that our services do not preclude
anyone from using the original manufacturer as a service provider. In fact,
under CIC's program, most facilities continue to use the service provider they
are most comfortable with, which is a popular feature of CIC's asset
management services.

In summary, the program works as follows:

CIC takes your existing service and maintenance contract(s) and combines them
into one comprehensive asset management agreement. In the process, you save
15 to 25% over manufacturer service contracts. When a service event occurs,
you call the service vendor OF YOUR CHOICE, who comes out and effects the
repair as usual. Upon receipt of the invoice, you simply submit it to CIC who
pays the service vendor directly, or reimburses you after you pay it,
depending on the terms of our agreement. In exchange, you pay CIC a fixed,
budgetable amount that is (again) 15 to 25% below what your facility was
paying.

I am unsure as to whether we would be allowed to subscribe to your list, as
many lists preclude private companies from subscribing. I would be greatly
appreciative if any misconceptions about our company and the service we
provide could be corrected. We are very proud of the efficiency and
cost-effectiveness we bring to facilities with high-tech equipment, and we are
also proud of the many endorsements we hold from major universities and
hospitals across the U.S. and Canada. Currently, we provide such services to
the University of Florida, the University of Ohio, Texas A&M, Cornell,
Colorado State, Louisiana State, UC-Davis, the University of Georgia, and
Virginia Tech. In addition, there are hundreds of hospitals in our client
base as well.

Perhaps you could copy my message to your list? Either way, thank you for
your inquiry and I will pass along your questions to a CIC Regional Manager in
your area. Thanks again.

Sincerely,

Bob Yancy
Director of Marketing
CIC Agency






From: luciom-at-NEWTON.UMSL.EDU (Luciano Mule'Stagno)
Date: Tue, 28 May 1996 10:34:57 -0500
Subject: microscopy of silicon

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Hi all,
I am interested in getting in touch with other people doing microscopy
work (research or routine) of silicon and possibly other semiconductors, for
sharing of experiences, and possible collaboration.


I am currently working on:
better understanding the role of bulk microdefectsa in the gettering
properties of silicon.
(TEM, Optical, lifetime , scanning Infra-Red Microscopes)

fundamental understanding of crystal growth - vacancy and interstitial
incorporation, grown in defects etc..
(tem, optical etc..)

SOI - interface, diffusion through etc..
(TEM)

developement on IR scanning tools for use in semiconductor bulk defect
characterization.

I am also interested in a variety of other areas of semiconductor research,
including problems related to device growth (metallization, oxide-related,
implantation etc..).

I work at MEMC Electronic Materials Inc, which is one of the world's
largest silicon wafer maker.
Thus obtaining material (and funding if the problem is relevant) is usually
not a problem.

cheers

Lucio


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~
Lucio Mule'Stagno
MEMC Electronic Materials Inc University of
Missouri -St.Louis
Silicon Materials Research Group Physics Dept.,
501 Pearl Dr., 8001 Natural
Bridge rd.,
St.Peters, St.Louis
MO 63376 MO 63121
tel: 314 279 5338 314 516 5931/3
fax: 314 279 5363 314 516 6152
email: lmulestagno-at-memc.com
luciom-at-newton.umsl.edu
URL:http://www.newton.umsl.edu
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
~~~~~~~~~~~~~~~~~~~~





From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 28 May 1996 18:38:52 -0500
Subject: GratefulMed/PPC7500

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Message-ID: {n1378843917.3500-at-quickmail.llnl.gov}
"South Bay Technology" {73531.1344-at-CompuServe.COM}
X-Mailer: Mail*Link SMTP-QM 3.0.2

RE} TEM: Dimpling Ti with SiC fibers 5/28/96

A number of years ago we discovered that CBN (cubic-Boron Nitride) will dimple
metals such as Al, Ti, Zr, Ta, Pt, Cu, Ni,...etc. much faster, with less
damage and smearing than diamond. This is for two possible reasons. Diamond
can chemically react with the metals and become bonded to the sample, thus
preventing any further dimpling, in fact you may find that your dimpling wheel
(steel, phosphor bronze, etc.) will actually wear away faster. Second, as the
abrasive action is taking place during the dimpling the diamond powders become
covered with the removed sample material and you are no longer dimpling with
diamond but with particle coated with metal-hence smearing and gauling. CBN
particles have different shaped corners and edges and will not react with the
metals. It is still necessary to change slurries more often when dimpling
metals and the process is slower that say dimpling Silicon.

CBN of course will not dimple SiC very well. The trick is to mix the CBN with
the diamond in the relative proportion of the sample composition. We have
found it possible to dimple two phase materials of vastly different hardness
at nearly identical rates using this multi-abrasive slurrey technique. We
start out with 2-4um then finish with 0-2um. We do not polish with a soft
cloth, this will instantly cause relief between the soft and hard phase.

Good luck

Mark Wall
Lawrence Livermore National Lab
510-423-7162

--------------------------------------

You've been so helpful in the past that I thought I'd give it another go!

I am looking for any help I can get on dimpling Ti with SiC fibers. I don't
have much more information than that at this point as I am relaying this
message
for someone else. If anyone can help, I'd really appreciate it.

While dimpling was the question, if you have information on Tripod Polishing,
ion milling, or any other technique that would also be quite useful.

Thank you very much!

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: 73531.1344-at-compuserve.com
sbt-at-msa.microscopy.com
http://www.southbaytech.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.


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Grateful Med (NLM) computer reference search program for the Mac V2.2.
I just upgraded to Mac system 7.5.3 which comes with Open transport 1.1. In
trying to start GM 2.2 there is a crash every time. I tried everything that
makes sense to solve the problem and communicated the techn services at the
NLM with no results. Anyone with similar problems and possible fixes please
contact me direclty.

*********************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} ----} Prof. Pathology & Otolaryngology *
*http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html*
*********************************************************************




From: shaun.sandow-at-anu.edu.au (Shaun Sandow)
Date: Wed, 29 May 1996 12:06:06 +1000
Subject: blocking elastin immunostaining

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Dear All,

Does anyone know of a blocking procedure for elastin in frozen thick
cryostat sections. Specifically we're using anti-rabbit FITC and are
getting non-specific staining of elastin, which we would like to block. The
tissue (rat tail artery and mesenteric artery) was fixed in 4%
parformaldehyde and embedded in Tissuetek. This is'nt directly an EM
question, but I thought someone might know anyway.........

Thanks,
______________________________________________

Shaun Sandow
Autonomic Synapse Group
Division of Neuroscience
John Curtin School of Medical Research
Australian National University
ACT 0200

email: shaun.sandow-at-anu.edu.au
Ph. (06) 249 4782 (work) (06) 247 6430 (home)
Fax. (06) 249 2687
Email: shaun.sandow-at-anu.edu.au





From: BARBARA.HARTMAN-at-spcorp.com (BARBARA HARTMAN)
Date: Wed, 29 May 1996 09:04:00 -0400
Subject: Immuno of Bacteria

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Mime-Version: 1.0

I have been asked to do immuno on bacteria (E. Coli). Has anyone had
any experience with this ? What fixative is the best to use ? Should
I do pre or post labeling ? I have tried two fixatives with this
bacteria and 2% Glut worked better than 0.5% Glut and 4% Para.
Anybody have any suggestions ? Thank you !!

Barbara Hartman
Schering-Plough Research Institute
201-579-4343
201-579-4211 (FAX)




From: Neal Nicklaus :      nnicklaus-at-nova.sarnoff.com
Date: Wed, 29 May 1996 09:45:26 -0400
Subject: Immuno of Bacteria

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Does anyone know of "attachments" for Zeiss microscopes which add confocal
laser scanning abilities?

I have both upright and inverted Zeiss microscopes. Both use the infinity
corrected, ICS, optics. I want to be able to use these objectives amongst
my various microscopes.

P.S. This question will be placed with both the confocal microscopy group
and the microscopy group.

P.P.S. I had previously asked about video rate confocal attachments. This
inquiry is for a different application (surface studies in reflection mode).


Neal Nicklaus

SEQ Limited

Voice: 609-452-6033 Ext. 13
Fax: 609-452-5955
email nnicklaus-at-seq.sarnoff.com





From: Neal Nicklaus :      nnicklaus-at-nova.sarnoff.com
Date: Wed, 29 May 1996 09:50:47 -0400
Subject: Adding Laser Confocal Scanning to a Microscope

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Does anyone know of "attachments" for Zeiss microscopes which add confocal
laser scanning abilities?

I have both upright and inverted Zeiss microscopes. Both use the infinity
corrected, ICS, optics. I want to be able to use these objectives amongst
my various microscopes.

P.S. This question will be placed with both the confocal microscopy group
and the microscopy group.

P.P.S. I had previously asked about video rate confocal attachments. This
inquiry is for a different application (surface studies in reflection mode).

P.P.P.S.: 2nd time I sent this to Microscopy Group - forgot to add subject
to the first transmission.



Neal Nicklaus

SEQ Limited

Voice: 609-452-6033 Ext. 13
Fax: 609-452-5955
email nnicklaus-at-seq.sarnoff.com





From: MELSEN :      MELSEN-at-MICROBIO.emory.edu
Date: Wed, 29 May 1996 12:30:09 EST
Subject: unsubscribe

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unsubscribe





From: Mike Witcomb :      MIKEW-at-gecko.biol.wits.ac.za
Date: Wed, 29 May 1996 18:10:52 GMT+2
Subject: CBED +-g set-up

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Would appreciate any help with the following:
Am trying space group determination for the first time. If I
understand correctly, which is questionable, for +G and -G conditions
you move the condenser aperture to the Bragg condition which would be
halfway between the bright field and the dark field disc at the zone
axis orientation. In one direction is OK, with little leeway, in the
opposite direction a severe cut-off occurs on all discs, say 50-70%.
It appears that this is a fixed aperture or polepiece (CM20). So
where have I stumbled? (Condenser aperture size 200 micrometres)
Thanks
Mike Witcomb


Dr MJ Witcomb
Electron Microscope Unit
University of the Witwatersrand
Private Bag 3
WITS
2050
South Africa

Telephone: + 27 11 716 4000
+ 27 11 716 2419 (messages)
Fax: + 27 11 339 3407
E-mail: mikew-at-gecko.biol.wits.ac.za




From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Wed, 29 May 1996 08:46:15 -0500
Subject: Re: TEM em-markers

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} } Your researcher might consider magnetic microspheres, since they are loaded
} with iron and are quite visible at the TEM. I have embedded and section
} them using routine techniques. Also most of these plastic microsperes have
} reactive surfaces so you might be able to attach a protein that has been
} coupled to small colloidal gold
} }
} Greg Erdos

I would go with the latter recommendation, as opposed to iron or
osmium (or other heavy metal) loaded microspheres. Aside from possible
reactivity/bodily reaction problems due to the heavy metals, the metals
will chang the specific gravity, and therefore flow characteristics of the
spheres. This would change their distribution within the blood vessels, and
so possibly affect where, how, and if they pass out of the vessels into the
surrounding tissues.

&&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&

Philip Oshel
soon-to-be-closed
Center for Electron Microscopy
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

************ looking for a job again
****************************************






From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Wed, 29 May 1996 11:39:05 -0700 (PDT)
Subject: Insect Fixation

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A standard fixation technique in the drosophila world requires several
steps: 1) dissolve the chorion with ~5% Na hypochlorite
(bleach) ~5 minutes then rinse usually with PBS and .3% Trition X-100; 2)
fix in a solution of heptane and your choice of buffered aldehyde;3) pipet
the samples off the interface and transfer to a heptane:methanol and shake
vigorously--the vitelline membrane will burst when the tissue swells. 4)
collect the samples that sink to the bottom and rehydrate in beffer. This
technique is called phase-partition fixation. See M. Zalokar & I Erk,
"Phase-Partition Fixation and Staining of Drosophila Eggs", Stain
Technology, Vol 52, No. 2, 1977, pp. 89-95.

Note the above is for embryos--it may not work with larvae that have a
more developed membrane. I usually slice very small larvae in half or
at the tip while submerged in fix or if they are larger I pin them out and
dissect them as appropriate. This requires a very sharp blade and good
microdissection technique--most grad students and post-docs can do it.
Contact me directly for more tips and hints.

Larry Ackerman mishot-at-itsa.ucsf.edu
The Laboratories of Lily & Yuh Nung Jan Voice (415) 476-8751
Howard Hughes Medical Institute
UCSF, Box 0724, Rm U426 FAX (415) 476-5774
533 Parnassus Ave.
San Francisco, CA 94143






From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Wed, 29 May 1996 16:17:18 -0600
Subject: LM/chloral hydrate + quenching

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Greetings,
Does anyone know if it's true that chloral hydrate actively
quenches fluorescence??? We found that some whole mount preps that had been
cleared in chloral hydrate were non-fluorescent, and I have heard vaguely
about quenching by chloral h. I was wondering if anyone had any similar
experience, or understood why from first principles such a molecule would
be expected to quench. Or are there folks out there who get great
fluorescence from specimens cleared in chloral h.
Many thanks,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Wed, 29 May 1996 19:16:49 -0300 (EST)
Subject: LM: used phase contrast microscope

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I'm searching for used phase contrast microscopes to
buy. I will be very grateful for any information.

Francisco J. Hernandez-Blazquez
fjhblazq-at-spider.usp.br
fjhblasq-at-biomed.icb2.usp.br







From: Kathy427-at-aol.com
Date: Wed, 29 May 1996 19:17:13 -0400
Subject: Comment/Question about Service Contracts

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There has been many comments and questions about CIC service.

My comment/question is since you would no longer have a service contract with
the manufacture, is the same response time available?

Most companies put the service contract customers first, hence quick response
time. Since this would no longer be the case, the question now is how long
can one wait for service. I have a feeling it may be quite some time, maybe
even weeks. A long wait can be very costly in down time. So in reality I
think there would not be a savings, more like a loss.

Kathy




From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 29 May 1996 18:21:28 -0500
Subject: Crashed fixed-Eureka!

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Message-ID: {n1378728056.54121-at-msmail.tmc.tulane.edu}
"Nash, Germaine" {snash-at-mailhost.tcs.tulane.edu}
Cc: "GratMed (Kathi Canese)" {gmhelp-at-nlm.nih.gov} ,
"Harrison, Jim " {harrison-at-TMC.Tulane.Edu} ,
"NIHImageUsers" {nih-image-at-soils.umn.edu} ,
"Wilson, James Robert" {jwilson-at-mailhost.tcs.tulane.edu}
X-Mailer: Mail*Link SMTP-MS 3.0.2

Eric is the champion. His suggestion fixed the problem. Solution, removed
the open transport that comes with the 7.5.3 upgrade and do a custom install
as described by Eric. What a relief! T H A N K S E R I C! ! ! !
} Grateful Med (NLM) computer reference search program for the Mac V2.2.
} I just upgraded to Mac system 7.5.3 which comes with Open transport 1.1. In
} trying to start GM 2.2 there is a crash every time.

You are not going to like hearing this but you will have to remove the
OT1.1 software and step back to the normal communications extensions you
were using pre-7.5.3. Open Transport has a mass of new extensions that are
not compatible with the majority of communications packages currently
available.

---------------------------------------------------------------
Eric Chapman - Eagle River Alaska _/_/_/ _/_/_/ _/_/
Hemodialysis Patient _/ _/ _/ _/ _/
_/ _/_/ _/
Sic Gorgiamus Alles _/ _/ _/ _/ _/
Subjectamos Nunc _/_/ _/_/_/ _/_/
---------------------------------------------------------------
The easiest method is to go into your system folder and delete all open
trasport extensions. Then, use a pre-7.5.3 install disk or CD and use
custom install. Just install the communications toolbox extensions and you
should be in business. We have a few of the new 7500 boxes in our shop here
and I will run over and talk to them about the problems we have been having
with open transport. I will get back to you before the end of the day.

Eric





From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Wed, 29 May 1996 22:10:35 -0500
Subject: Authors/Titles for Microscopy & Microanalysis-96 On-Line

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Colleagues,

A complete listing of all Authors and Manuscript Titles for
Papers and Posters which will be presented at the Microscopy
& Microanalysis-96 Meeting in Minneapolis, Minnesota
August 11-15, 1996 is now on-line at the URL

http://www.msa.microscopy.com

Look for the hot link entitled "Author/Title Index" under
the Annual Meetings Area.


See you in Minneapolis...........

Nestor
Your Friendly Neighborhood SysOp
(& Program Chairman- Microscopy & Microanalysis-96)






From: Elinor Solit :      cambrex-at-world.std.com
Date: Thu, 30 May 1996 11:22:20 -0400 (EDT)
Subject: Re: LM: used phase contrast microscope

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Francisco,

Try the Zeiss people at their US headquarters: 800-356-1090.
You may be able to obtain more information from their Web site. Access
it through ours: http://www.shore.net/~catalogs. You'll find many
products featured there. If you call, please tell them we sent you.

Best of luck,

Elinor Solit,
The Microscope Book

On Wed, 29 May 1996, Francisco J. Hernandez wrote:

} I'm searching for used phase contrast microscopes to
} buy. I will be very grateful for any information.
}
} Francisco J. Hernandez-Blazquez
} fjhblazq-at-spider.usp.br
} fjhblasq-at-biomed.icb2.usp.br
}
}
}




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Thu, 30 May 1996 13:14:49, -0500
Subject: ISO Guide 25/Quality issues

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X-Nupop-Charset: English

-- [ From: Garber, Charles A. * EMC.Ver #2.10P ] --

The American Association for Laboratory Accreditation (A2LA) has
announced a new listserver for the discussion of issues relating to
laboratory accreditation. Specifically:

"Who is doing what, how and why organizations are becoming accredited,
what the successes and concerns have been, what concepts, methods,
software and organizatons have been beneficial, etc"

Subscription information can be found at the following URL:

http://www.fasor.com/~iso25/listserv.html

The A2LA home page is accessible from the above URL code. If anyone
thinks laboratories are not being accredited, they would have to be
impressed with the long listing of laboratories under current
accrreditation by A2LA. More than a few EM oriented laboratories are
covered by the A2LA accreditation program, including our own
laboratory. What ever money we have had to spend to maintain our
accreditation program, we are completely convinced comes back to us in
the form of significantly lower costs associated with "rework" (e. g.
having to do work over again because it was not done right the first
time).

Chuck

Hello Charlie:

I am sending this non-list server comment to you, off the listsever in
order that my comments not be misinterpreted by anyone since we are one
of the several main sellers of osmium tetroxide to the world wide EM
community.

At the risk of appearing to be not so knowledgeable, let me give you my
own thoughts on this issue:

a) There is no reason why freezing aqueous osmium tetroxide should in
any way, shape, or form degrade the "quality" of the material in the
vial. Therefore it would not surprise me that no one reported any
erosion of quality.

b) My biggest fear has been from both a safety and also product
liability standpoint. The safety aspect comes into play because as the
aqueous component drops through 4 deg. C, where there is a maximum in
the expansion curve, it just seemed to me to be the classic accident
waiting to happen: Any ampoule with glass containing even the
slightest defect (would be a major stress riser), and with the glass
being so brittle, I would expect that the ampoule would spontaneously
shatter.

I just can't imagine a worse kind of clean up problem and depending on
when it happened and when it was discovered, who knows what other kind
of damage could occur. Or what kind of injury could occur. Of course,
there was one posting that indeed did report a broken ampoule in the
freezer, the failure being presumably due to the freezing.

From a product liability standpoint, I would be very reluctant to
recommend this as a method of storage. I admit that this is
"defensive" business practice, but I sure don't want to have to start
hiring lawyers to defend our firm against legal action, in the event we
should have suggested freezing as an acceptable method of storage and
have it then result in an accident and damage, worse yet, some kind of
human injury.

If someone really did want to store the osmium tetroxide (aqueous) in a
freezer, then there are plastic sleeves that I would recommend they be
place in, if for no other reason that in the event there should be some
kind of a failure of the glass ampoule, then the plastic sleeve would
at least tend to contain any osmium from getting out into the general
part of the refrigerator.

Now for what might at least have the appearance of a commercial pitch,
and I don't mean it to be such a "pitch", we have been offering aqueous
osmium tetroxide for more than twenty years. If one practices ultra
clean practices, both in terms of the glass ampoule before filling and
also with the water diluent (all the osmium starts out with the same
starting purity), and also the use of a high quality dry nitrogen for
filling the head space, then the product should last virtually
indefinitely without refrigeration! Yes, that is right. Now not all
people offering aqueous osmium tetroxide are ampuoling it to that
standard as evidenced by the fact that people have had problems and
have resorted to freezing it in order to extend the shelf life. I am
almost certain that our aqueous osmium tetroxide is not the cheapest
around, but people who have been using it, just don't have to go to
such lengths in order to keep it active and in the long term, the
higher stability is well worth any few pennies more in the selling
price.

My own gut feeling is that the typical end user should just be
purchasing aqueous osmium tetroxide of the highest quality and avoid
altogether the safety and other risks associated with the freezing in
a glass ampoule this really hazardous material.

You have my permission to re-post this message on the listserver, if
you think it would be of more general interest. You also have my
permission to edit it as you see fit.

Best regards.


Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail:
GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service:spi2spi-at-2spi.com

########################################################
WWW: http://www.2spi.com
########################################################
======================================================




From: Ian MacLaren :      MACLARIZ-at-novell2.bham.ac.uk
Date: 30 May 1996 16:41:22
Subject: High res printers

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To: Microscopy-at-Sparc5.Microscopy.Com

Dear all,
I realise that this question has been asked in the recent past but I didn't
think to keep the responses.

What are peoples experiences with the use of high res laser printers or
other types of printer for printing EM images? Do you have any printer
that you would recommend? I would be interested in using it for printing
out a wide variety of TEM and SEM images of materials science specimens.

I would also be very glad if anyone has a summary of previous messages that
have been posted on this subject that they could forward to me.

Thanks

_________________________________________________________________
Ian MacLaren, Telephone: 0121 414 3447
IRC in Materials, FAX: 0121 414 3441
The University of Birmingham, email: I.MacLaren-at-bham.ac.uk
Birmingham B15 2TT, England.
_________________________________________________________________




From: Igor Polyakov :      Igor_Polyakov-at-qmgate.arc.nasa.gov
Date: 30 May 1996 16:12:34 -0800
Subject: unsubscribe

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Subject: Time:4:13 PM
OFFICE MEMO unsubscribe Date:5/30/96

unsubscribe
Igor_Polyakov-at-qmgate.nasa.arc.gov





From: Greg2NJ-at-aol.com
Date: Thu, 30 May 1996 20:20:36 -0400
Subject: looking for Best Image Analysis system

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In everyone’s opinion, what is the best commercial Image processing and
analysis package available on the market today.
One that offers excellent support, intuitive ease of use, fully editable
scripts or macros, and can handle a wide variety of applications.
Currently, I am using Kontrons IBAS v2.0, thinking about upgrading to a new
system.
Thanks
Gregory Argentieri
Sandoz Pharmaceuticals Corp
201-503-8617
Fax 201-503-6339





From: trevarro-at-uoneuro.uoregon.edu
Date: Thu, 30 May 1996 17:23:02 -0800
Subject: Re: Microscope booking software

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} We currently have users book microscope time by writing with a pen on
} booking sheets made of paper. We are contemplating changing this antiquated
} system to a computer booking system which would permit people to book (and
} to check existing bookings) over the net.
}
} Does anyone know of software to do this? Please take into account that we
} would want the software to meet the following conditions:
}
} It should work for several instruments.
} It should prevent one user erasing another's booking.
} It should allow staff to change the bookings of users.
} If a user deletes his or her own booking, a record of the booking and the
} time of the cancellation should be kept.
} It should be possible to program booking rules into the system. These rules
} may be different for each instrument. The rules would include such things
} as how many sessions may be booked at any one time and how long a session
} may be booked.
} It should be possible to include various degrees of access for the
} users. Some users may be allowed to book evenings and weekends while others
} may only book daytime sessions.
}
} We feel that unless the software we use can meet most of these requirements,
} we will be better off staying with pen and paper. Your suggestions would be
} most welcome.
} Alwyn Eades eades-at-uimrl7.mrl.uiuc.edu
}
} Center for Microanalysis of Materials, University of Illinois,
} 104 S. Goodwin, Urbana, Illinois, 61801-2985, USA
} 217-333-8396, Fax 217-244-2278.

You should be able to do all of this by networking one or more database
files by using a database manager such as Filemaker Pro (Claris).

Specifically:
} It should work for several instruments.
you could use several different files.
} It should prevent one user erasing another's booking.
by using access privileges and pass words you can restrict what particular
users could see or do in different layouts
} It should allow staff to change the bookings of users.
this could be allowed by their password and access privileges
} If a user deletes his or her own booking, a record of the booking and the
} time of the cancellation should be kept.
A enw current record could be made and the old one kept.
} It should be possible to program booking rules into the system. These rules
} may be different for each instrument. The rules would include such things
} as how many sessions may be booked at any one time and how long a session
} may be booked.
different files with different sets of access privileges.
} It should be possible to include various degrees of access for the
} users. Some users may be allowed to book evenings and weekends while others
} may only book daytime sessions.
different access privileges for different sets of users.

The drawback to this approach is that a copy of the program (or site
license) all the user computers (or at least for all of those running at
once) should be purchased in order to be legal. Unless your campus has a
license.
On the other hand database managers can be used to maintain several
different files.

Bill

Bill Trevarrow
Institute of Neuroscience
University of Oregon 1254
Eugene, OR 97403-1254
Off.Tel: (541) 346-4598
Fac. Tel: (541) 346-4512
Fax: (541) 346-4548
e-mail: trevarro-at-uoneuro.uoregon.edu






From: Mike Folsom :      mwfolsom-at-unm.edu
Date: Thu, 30 May 1996 21:58:56 -0600 (MDT)
Subject: Re: looking for Best Image Analysis system

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On Thu, 30 May 1996 Greg2NJ-at-aol.com wrote:

} In everyone=92s opinion, what is the best commercial Image processing and
} analysis package available on the market today.=20
} One that offers excellent support, intuitive ease of use, fully editable
} scripts or macros, and can handle a wide variety of applications.=20
} Currently, I am using Kontrons IBAS v2.0, thinking about upgrading to a n=
ew
} system.
} Thanks
} Gregory Argentieri
} Sandoz Pharmaceuticals Corp
} 201-503-8617
} Fax 201-503-6339
} =20
} =20

That's easy -

For 2D - MetaMorph by Universal Imaging
For 3D - VoxelView by Vital Imaging

Michael

Dept. of Biology
Univ.of New Mexico
Albuquerque, NM 87131





From: jan_ringnalda-at-pei.philips.com (jan ringnalda)
Date: Fri, 31 May 1996 01:34:58 -0400
Subject: Re: High res printers

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Mime-Version: 1.0
Ian MacLaren {MACLARIZ-at-novell2.bham.ac.uk}
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
Content-Description: cc:Mail note part

I've used the Tektronics phaser 2sdx with good success, but I believe
the kodak printers are heading for the top rapidly.
Cheers, Jan




From: Lars.Bjork :      lasse-at-UKWANGELA.imm2.su.se
Date: Fri, 31 May 1996 11:05:42 +0200 (MET DST)
Subject: Re: looking for Best Image Analysis system

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On Thu, 30 May 1996 Greg2NJ-at-aol.com wrote:

} In everyones opinion, what is the best commercial Image processing and
} analysis package available on the market today.
} One that offers excellent support, intuitive ease of use, fully editable
} scripts or macros, and can handle a wide variety of applications.
} Currently, I am using Kontrons IBAS v2.0, thinking about upgrading to a new
} system.
} Thanks
} Gregory Argentieri
} Sandoz Pharmaceuticals Corp
} 201-503-8617
} Fax 201-503-6339
}
}


Personally, I have had a very good experience with the new Quantimet Q600
system from Leica. It is a fast traditional image analysis system (like the
IBAS) but is user friendly and the macros can be written with the mouse
(very simple). In the high resolution mode it can handle images above
1K X 1K pixel.

My two cents,

Lars Bjork
Dept Immunology
Wenner-Gren Institute
Stockholm University
SWEDEN




From: howard-at-cshl.org (Tamara Howard in Cold Spring Harbor Laboratory)
Date: Fri, 31 May 1996 08:02:46 -0500
Subject: LM/TEM/plants/seeds

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Message-Id: {9605311255.AA12167-at-phage.cshl.org}
X-Sender: howard-at-phage.cshl.org
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Helpful people:
I'm having trouble with some seed material...we need to look at the
pericarp(s) of these guys through the seeds' development. The early stages
were cake, but I'm having trouble with older material - it is more "woody"
as we go. I've found several seed prep protocols, but I was hoping someone
out there has a tried and true method...
Thanks!
Tamara Howard
CSHL






From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Fri, 31 May 1996 08:32:51 -500
Subject: Re: TEM em-markers

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Here's another suggestion I'd like to in the mix. I worked with a
materials person a few years ago doing some TEM on mixtures of
polymeric materials. The one I remember was a mixture of Polysytrene
and Starch. I thought we were going to have real problems visually
separating the two materials in the TEM thin sections, and was ready
to play all sorts of staining games. But he took the first step and
simply sectioned the material (unfixed, unembedded) and popped them
into the scope and we had absolutely no problem seeing them in the
scope! One phase was nearly electron transparent (light grey) and
one phase was nearly electron opaque (dark grey - sorry I do not
remember which one was which).

Before trying weird stains and possible immuno labeling horrors:
Have you tried emmbeding the spheres (or serval possible different
material types) sectioning them and looking to see what shows up ijn
the TEM? I would think the nice, neat, very uniform density circles
should be fairly easy to find in amongst the 'mess' of biological
material in the scope.

(I am a Biologist - primarily - and any one offended by refering to
a 'mess of biological material' needs to relax and get their sense of
hummor back.)


Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu




From: Luc Nocente :      ln-at-noesisvision.com
Date: Fri, 31 May 1996 09:20:11 -0400
Subject: Re: looking for Best Image Analysis system

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Message-Id: {2.2.32.19960531132011.00751be8-at-noesisvision.com}
X-Sender: ln-at-noesisvision.com
X-Mailer: Windows Eudora Pro Version 2.2 (32)
Mime-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable

At 08:20 PM 5/30/96 -0400, you wrote:
} In everyone=92s opinion, what is the best commercial Image processing and
} analysis package available on the market today.=20
} One that offers excellent support, intuitive ease of use, fully editable
} scripts or macros, and can handle a wide variety of applications.=20
} Currently, I am using Kontrons IBAS v2.0, thinking about upgrading to a new
} system.
} Thanks
} Gregory Argentieri
} Sandoz Pharmaceuticals Corp
} 201-503-8617
} Fax 201-503-6339
}
}


You can try Visilog from Noesis Vision Inc. We have been developing
software for over 11 years and are in the process of introducing a brand new
32 bit version compatible with Windows 95 and NT. Visilog runs on both PC
and Unix workstations and provides a wide selection of image processing
algorithms, macro language(editable), a redone easy to use GUI (user
interface)and drivers for various grabbers and stages. Visilog is widely
used in the microscopy field.

=
=20
----------------------------------------------------------
---------------------------------------
Luc Nocente Tel: 514 345 1400
Noesis Vision Inc. Fax: 514 345 1575
e-mail: ln-at-noesisvision.com http://www.cam.org/~noesis
6800 Cote de Liesse, Suite 200
St-Laurent, PQ
H4T 2A7,Canada

Join the Noesis NewsGroup at Visilog-at-noesisvision.com
----------------------------------------------------------------------------
---------------------





From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Fri, 31 May 1996 09:02:11 GMT
Subject: Re: High res printers

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At 04:41 PM 5/30/96, you wrote:
} Dear all,
} I realise that this question has been asked in the recent past but I didn't
} think to keep the responses.
}
} What are peoples experiences with the use of high res laser printers or
} other types of printer for printing EM images? Do you have any printer
} that you would recommend? I would be interested in using it for printing
} out a wide variety of TEM and SEM images of materials science specimens.
}
} I would also be very glad if anyone has a summary of previous messages that
} have been posted on this subject that they could forward to me.
}
} Thanks
}
} _________________________________________________________________
} Ian MacLaren, Telephone: 0121 414 3447
} IRC in Materials, FAX: 0121 414 3441
} The University of Birmingham, email: I.MacLaren-at-bham.ac.uk
} Birmingham B15 2TT, England.
} _________________________________________________________________
}
}

Hi Ian.
If you have www access, then I the man you want to hear from. Go to the
address listed at the bottom of this message and look in the "Tips & Tricks"
section. There you will find a link to a set of links to a set of links...
Look for the "Image processing, conversion, and software" link and you will
find a recent discussion that came across the list. If for some reason you
do not have web access, get it , or e-mail me back and I will get the file
to you.
As an answer, we have a Lexmark Optra R, The cheap, get them proofs,
1200 dpi laser and the Kodak xls 8600 dye sub, bet you can't tell which is
the photograph printers. Both have been excellent and just recently
withstood the battering only a group of inexperienced students could dish
out flawlessly. In all we have been pleased. Hope this helps.





} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/





From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Fri, 31 May 1996 09:04:44 -0500
Subject: Re: Microscope booking software

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Responding to the message of {v01510103add3f6f4208b-at-[128.223.140.24]}
from trevarro-at-uoneuro.uoregon.edu:
}
} } We currently have users book microscope time by writing with a pen on
} } booking sheets made of paper. We are contemplating changing this antiquated
} } system to a computer booking system which would permit people to book (and
} } to check existing bookings) over the net.
} }
} } Does anyone know of software to do this? Please take into account that we
} } would want the software to meet the following conditions:
} }

} You should be able to do all of this by networking one or more database
} files by using a database manager such as Filemaker Pro (Claris).
}
} Specifically:
}
} } It should prevent one user erasing another's booking.
} by using access privileges and pass words you can restrict what particular
} users could see or do in different layouts

I have found it very difficult to get Filemaker Pro to do something like this.
We have attempted to get around this problem by having a Web browser front end
that communicates with Filemaker Pro using an applescript cgi which does the
access privelege part.

The disadvantage of the system is that, even for a University, the number of
copies of Filemaker we have (one for each microscope for logging of hours rather
than booking of time) represents a ridiculous expense. We could have a Web
browser do the logging part too, but suspect that it will get used for Web
browsing rather than data entry!

The main advantage of the system is that relatively little human intervention is
necessary for the generation of monthly billing statements.

Stuart McKernan stuartm-at-maroon.tc.umn.edu
CIE Microscopy Center, University of Minnesota Office: (612) 626-7942
100 Union Street S. E., Minneapolis, MN 55455 Lab: (612) 624-6590





From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Fri, 31 May 1996 09:14:55 -0500
Subject: Re: Microscope booking software

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Message-ID: {31AEFEDF.55DD-at-jagunet.com}

alwyn eades wrote:
}
} We currently have users book microscope time by writing with a pen on
} booking sheets made of paper. We are contemplating changing this antiquated
} system to a computer booking system which would permit people to book (and
} to check existing bookings) over the net.
}
} Does anyone know of software to do this? Please take into account that we
} would want the software to meet the following conditions:
}
} It should work for several instruments.
} It should prevent one user erasing another's booking.
} It should allow staff to change the bookings of users.
} If a user deletes his or her own booking, a record of the booking and the
} time of the cancellation should be kept.
} It should be possible to program booking rules into the system. These rules
} may be different for each instrument. The rules would include such things
} as how many sessions may be booked at any one time and how long a session
} may be booked.
} It should be possible to include various degrees of access for the
} users. Some users may be allowed to book evenings and weekends while others
} may only book daytime sessions.
}
} We feel that unless the software we use can meet most of these requirements,
} we will be better off staying with pen and paper. Your suggestions would be
} most welcome.
} Alwyn Eades eades-at-uimrl7.mrl.uiuc.edu
}
} Center for Microanalysis of Materials, University of Illinois,
} 104 S. Goodwin, Urbana, Illinois, 61801-2985, USA
} 217-333-8396, Fax 217-244-2278.

Alwyn,

Try FileMaker Pro by Claris. It is available for Windows, Windows95
and Macintosh. It is a fully relational, customizable, open
architecture database that has extensive password level protection. It
can be learned in a few hours and has endless capabilities. Trying to
find a specific software for "microscope sign-up" will be close to
impossible unless one of these kind folks on this server will share
one of their previous efforts. Even then you will find it is a
template from a relational database or spreadsheet.

Good Luck,

Lawrence Kordon
Nikon, Inc.
Columbia, Maryland
nikon-at-jagunet.com




From: loakford-at-hsc.unt.edu (Larry Oakford)
Date: Fri, 31 May 1996 15:12:19 -0500
Subject: Re: Comment/Question about Service Contracts

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} Date: Fri, 31 May 1996 14:59:10 -0500
} To:Kathy427-at-aol.com
} From:loakford-at-hsc.unt.edu (Larry Oakford)
} Subject:Re: Comment/Question about Service Contracts
} Cc:
}
} I concur wholeheartedly with Kathy's comments below. My
} understanding is that all non-service contract and non-installation work
} is last in line to get service from all major electron microscope service
} providers. It also means that if a contract call comes in while they are
} at your site they may have to leave and attend to it before getting back
} to your problem. I have known of non-service contract labs having to wait
} as long as 3 months to see a service engineer, and this was from a major
} TEM manufacturer. If possible extended down time is nondisruptive to the
} functioning of your lab then I would say go ahead with the CIC plan,
} otherwise fight with every breath in your body to maintain a standard
} service contract, even a modified one with less coverage or extended
} response times, most service organizations are willing to negotiate. You
} may get lucky and have a microscope that stays healthy and never needs to
} have a service call, but you may be like the individual I know who had to
} wait 3 months to get operational again while fending off users who needed
} to use the microscope. There is a lot of truth in the saying "you get
} what ya pays for". I hope this helps.
}
} P.S. It is also true, as pointed out by another writer, that if you decide
} to return to a service contract after the present one runs out, and you
} did not renew, it will cost you. You will need to go through a
} pre-service contract routine where all expenses(time, travel & parts) are
} directly charged above the cost of the new service contract (this can
} amount to an extra bill of $2000 or more). This is a standard practice in
} the microscope service industry concerning lapsed contracts, both
} manufacturers and independent providers alike.
}
} } There has been many comments and questions about CIC service.
} }
} } My comment/question is since you would no longer have a service contract with
} } the manufacture, is the same response time available?
} }
} } Most companies put the service contract customers first, hence quick response
} } time. Since this would no longer be the case, the question now is how long
} } can one wait for service. I have a feeling it may be quite some time, maybe
} } even weeks. A long wait can be very costly in down time. So in reality I
} } think there would not be a savings, more like a loss.
} }
} } Kathy
}

XXX
XXX
XXX
XXX
[]-XXX---
XXX
XXX
-------XXX--------
| o XOX [] |
-------XXX--------
| |
| |
------------------

Lawrence X. Oakford, Ph.D.
Department of Anatomy and Cell Biology
UNT Health Science Center
Fort Worth, TX 76107
e-mail:xavier-at-jove.acs.unt.edu






From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 5/30/96 10:07 PM
Subject: looking for Best Image Analysis system

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In everyone’s opinion, what is the best commercial Image processing and
analysis package available on the market today.
One that offers excellent support, intuitive ease of use, fully editable
scripts or macros, and can handle a wide variety of applications.
Currently, I am using Kontrons IBAS v2.0, thinking about upgrading to a new
system.
Thanks
Gregory Argentieri
Sandoz Pharmaceuticals Corp
201-503-8617
Fax 201-503-6339






From: Igor Polyakov :      Igor_Polyakov-at-qmgate.arc.nasa.gov
Date: 31 May 1996 16:47:39 -0800
Subject: unsubscribe

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Message-Id: {n1378561113.57254-at-qmgate.arc.nasa.gov}

Subject: Time:4:13 PM
OFFICE MEMO unsubscribe Date:5/30/96

unsubscribe
Igor_Polyakov-at-qmgate.nasa.arc.gov





From: John M. Libert :      jlibert-at-cpcug.org
Date: Sat, 01 Jun 1996 11:47:34 -0500
Subject: UNSUBSCRIBE

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Message-ID: {31B07426.74C8-at-cpcug.org}

UNSUBSCRIBE




From: Marc C. Brande, MS, Founder :      mcbrande-at-sierra.net
Date: Sat, 01 Jun 1996 12:02:44 +0000
Subject: Microlumina camera

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Message-Id: {31B03164.416E-at-sierra.net}

Would anyone have contact information for the Microlumina camera for
hi res digital imaging? Thanks so much

Marc




From: Probing & Structure :      pns-at-ultra.net.au
Date: Sun, 2 Jun 1996 21:08:58 +1000
Subject: Re: Microlumina camera

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At 12:02 01-06-96 +0000, you wrote:
} Would anyone have contact information for the Microlumina camera for
} hi res digital imaging? Thanks so much
}
} Marc
}
} That digital camera is available in North America through Electron
Microscopy Sciences (email: sgk-at-aol.com). We are the distributor in Australasia.
Jim Darley
Manager

Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
Internet Catalogue: http://www.ultra.net/~pns/





From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Sun, 2 Jun 1996 15:39:49 -0500
Subject: Microscopy & Microanalysis-96 Search Engines Running

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Message-Id: {199606022034.PAA01952-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Colleagues...

A Search Engine has now been implemented for the Scientific Program
of Microscopy & Microanalysis-96 . You may search this database by
Author Last Name or Keywords selected from the Manuscript Title.
The Search Engine is on-line at the Microscopy Society of America's
WWW site found at the URL:

http://www.msa.microscopy.com


In addition, Abstracts of the Contents of Volume 2 Issues 1&2
of the Journal of the Microscopy Society of America are also
now on-line, along with a few other new items which you might
find interesting.

As always please report problems that you might
find directly to me....



Cheers.. Nestor

Your Friendly Neighborhood SysOp
(also Microscopy & Microanalysis-96 Program Chairman)








From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Mon, 3 Jun 1996 10:04:15
Subject: Re: Microscope booking software

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To: microscopy-at-sparc5.microscopy.com

In article {31AEFEDF.55DD-at-jagunet.com} Lawrence Kordon {nikon-at-jagunet.com} writes:
} Date: Fri, 31 May 1996 09:14:55 -0500
} From: Lawrence Kordon {nikon-at-jagunet.com}
} Subject: Re: Microscope booking software

} alwyn eades wrote:
} }
} } We currently have users book microscope time by writing with a pen on
} } booking sheets made of paper. We are contemplating changing this antiquated
} } system to a computer booking system which would permit people to book (and
} } to check existing bookings) over the net.
} }
} } Does anyone know of software to do this? Please take into account that we
} } would want the software to meet the following conditions:
} }
} } It should work for several instruments.
} } It should prevent one user erasing another's booking.
} } It should allow staff to change the bookings of users.
} } If a user deletes his or her own booking, a record of the booking and the
} } time of the cancellation should be kept.
} } It should be possible to program booking rules into the system. These rules
} } may be different for each instrument. The rules would include such things
} } as how many sessions may be booked at any one time and how long a session
} } may be booked.
} } It should be possible to include various degrees of access for the
} } users. Some users may be allowed to book evenings and weekends while others
} } may only book daytime sessions.
} }
We implemented our NET=BOOK software to do precisely this about 4 years ago.
It was custom witten in C++ by our Chris Martinic and runs under DOS on our
network. It effortlessly handles bookings for 4 EMs, microtomes, coating
units, image processors, analysers, and so on.

It carries out the functions you prescribe. As it is custom made it can
be easily modified when further good ideas are proposed. Chris is off sick
today but I predict he will be back on deck in about 7 days and would be ready
to field further questions.

mel dickson
unsw.




From: Sally Shrom :      sally-at-retina.anatomy.upenn.edu
Date: Mon, 3 Jun 1996 09:26:04 -0400 (EDT)
Subject: TEM ultra microtomes

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Posted-Date: Mon, 3 Jun 1996 09:31:16 -0400

What was the conclusion about the best ultra microtome for ultra thin
sectioning of tissue embedded in epon?

Sally




From: hainfeld-at-genome1.bio.bnl.gov
Date: Mon, 3 Jun 1996 10:22:50 -0400
Subject: gold workshop

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Gold Cluster Labeling Workshop
(and STEM microscopy)

Sept. 18-21, 1996
Brookhaven National Lab
Upton, Long Island, NY

This is a hands-on lab (and lecture) course that covers labeling proteins
with Nanogold and Undecagold. Topics included: labeling chemistry and
strategies, labeling thiols and amines, thiol reduction, column
chromatography for separating unreacted gold, spectral
quantitation/stoichiometry of labeling, preparation of STEM and TEM
samples, use of low Z stains, STEM and TEM visualization, silver
enhancement of blots, running and detecting labeled proteins on gels, and
new labels. Some participants' samples will also be labeled.

Instructors: James Hainfeld, Richard Powell (Nanoprobes), Fred Furuya
(Nanoprobes), Joseph Wall (BNL), Martha Simon (BNL); guest presentations
from successful users

Cost: a registration fee of $500 to cover costs (includes materials,
dinners, coffee, evening refreshments)..
Housing: Dorms at $14.50 per night; or guest house $57/night.

Registration limited due to lab equipment; please indicate preliminary
interest by June 6, giving name(s) of those that would like to attend. If
sufficient interest, additional courses will follow.

The Brookhaven STEM is a NIH Biotechnology Resource.

Respond by June 6 to:
Jim Hainfeld, tel. 516-344-3372, fax. 516-344-3407,
email: hainfeld-at-genome1.bio.bnl.gov
(the #1 in genome1)






From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Mon, 3 Jun 1996 09:14:10 -0800
Subject: LM: pre-embedded fluorescent marker

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Message-Id: {v02110101add8ca8ed202-at-[130.191.238.29]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

howdy all

I have a user with lung tissue that has been labelled with a fluorescent
marker in vivo. She wants to examine light microscope slices (e.g.,
cryostat slices) for distribution of the marker. I believe most fixatives
containing Glutaraldehyde or formadehyde will quench the marker
fluorescence and/or add autofluorescence. Any suggestions on how to
prepare such tissue?

Can we simply plungefreeze small pieces in LN or propane?

Can we infiltrate the unfixed tissue first with sucrose as a
cryoprotectant? The air pockets will no doubt cause additional problems.
Would a quick exposure to a vacuum help the sucrose or fixative penetrate?


This request is a new one for me. Any and all suggestions would be appreciated

steve

Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego, CA 92182-4614
phone: (619)594-4523
fax:(619)594-5676
email:sbarlow-at-sunstroke.sdsu.edu






From: Marilyn Wadsworth :      mwadswor-at-MOOSE.UVM.EDU
Date: Mon, 3 Jun 1996 14:12:11 -0400 (EDT)
Subject: Databases

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Hello all,
We are interested in learning what kind of database people are finding
most successful for billing purposes and workload recording. We are
currently using a Paradox (DOS) which is networked between our imaging
facility and business office. Our billing tends to be complicated since
we have multiple users, budget numbers and equipment as well as multiple
people inputting information and we are looking for an efficient way to
streamline our operations. Any comments would be greatly appreciated,
either to me directly or to the list.

Regards,
Marilyn

+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
+ Marilyn Wadsworth | Phone: (802) 656-0813 +
+ Cell Imaging Facility | E-Mail: mwadswort-at-moose.uvm.edu +
+ School of Medicine, Path Dept | +
+ University of Vermont | +
+ Burlington, Vermont 05405 | +
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++




From: hainfeld-at-genome1.bio.bnl.gov
Date: Mon, 3 Jun 1996 14:59:30 -0400
Subject: please post this announcement

Contents Retrieved from Microscopy Listserver Archives
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Gold Cluster Labeling Workshop
(and STEM microscopy)

Sept. 18-21, 1996
Brookhaven National Lab
Upton, Long Island, NY

This is a hands-on lab (and lecture) course that covers labeling proteins
with Nanogold and Undecagold. Topics included: labeling chemistry and
strategies, labeling thiols and amines, thiol reduction, column
chromatography for separating unreacted gold, spectral
quantitation/stoichiometry of labeling, preparation of STEM and TEM
samples, use of low Z stains, STEM and TEM visualization, silver
enhancement of blots, running and detecting labeled proteins on gels, and
new labels. Some participants' samples will also be labeled.

Instructors: James Hainfeld, Richard Powell (Nanoprobes), Fred Furuya
(Nanoprobes), Joseph Wall (BNL), Martha Simon (BNL); guest presentations
from successful users

Cost: a registration fee of $500 to cover costs (includes materials,
dinners, coffee, evening refreshments)..
Housing: Dorms at $14.50 per night; or guest house $57/night.

Registration limited due to lab equipment; please indicate preliminary
interest by June 6, giving name(s) of those that would like to attend. If
sufficient interest, additional courses will follow.

The Brookhaven STEM is a NIH Biotechnology Resource.

Respond by June 6 to:
Jim Hainfeld, tel. 516-344-3372, fax. 516-344-3407,
email: hainfeld-at-genome1.bio.bnl.gov
(the #1 in genome1)






From: Marc C. Brande, MS, Founder (619) 587-4830 FAX: (619) 552-1516 :      mcbrande-at-sierra.net
Date: Mon, 03 Jun 1996 14:02:14 +0000
Subject: 2 Video Cameras on Same Port

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Is it possible to put 2 video cameras on the same port on any of the
research grade scope makes (Leica, Zeiss, Nikon, Olympus) so one can
easily switch between cameras for different lite mode capture? Thanks
in advance.

Marc




From: kszaruba-at-MMM.COM (Karen S. Zaruba)
Date: Mon, 03 Jun 1996 17:57:10 -0500
Subject: LM - Help on In Vivo/Situ Stains for Confocal

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Message-Id: {199606032253.AA108452380-at-pigseye.mmm.com}
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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

My request is similar, yet perhaps opposite to Dr. Barlow's [regarding
specimen preparation for tissue which has been fluorescently tagged in vivo].

We would like to use laser scanning confocal microscopy to visualize the
3D architecture of blood vessels in tissue whole mounts; then section
the same tissue for further immunohistochemistry. Does anyone know
of a method to label the vessels for confocal that would be compatible
with "routine" histological specimen preparation (i.e. formalin fixation
and paraffin embedding)? Perhaps there are non-fluorescent tracers
(minerals or reflective substances?) that would stand up to the processing??

Your help and ideas are appreciated!

Karen Zaruba

Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
3M Company
3M Center 270-1S-01 Phone: 612-737-2971
St. Paul, MN 55144-1000

These opinions are my own and may not represent those of 3M.







From: Terry.R.McCue-at-mcdermott.com
Date: 3 Jun 96 16:33:00 -0500
Subject: EPMA Room Temp ???

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Is there anyone out there who has experience or data regarding the
ambient temperture variations that can be tolerated by an ARL Electron
microprobe (with AMI " computer, scaler motor upgrade") ie. " counts
per degree per hour " or something like that. Recent demands in
responce to ISO and our instruments people who keep an eye on this
kind of stuff has made it an issue in the writing of our lab Tech.
Procedures. THANKS

Terry R. McCue
Babcock & Wilcox Research
1562 Beeson St.
Alliance, Ohio 44601
voice: (330) 829-7427
Fax : (330) 829-7831
internet: terry.r.mccue-at-rdd.mcdermott.com




From: tom moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Mon, 3 Jun 1996 16:47:24 -0500 (CDT)
Subject: Re: LM: pre-embedded fluorescent marker

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On Mon, 3 Jun 1996, Steve Barlow wrote:

} howdy all
}
} I have a user with lung tissue that has been labelled with a fluorescent
} marker in vivo. She wants to examine light microscope slices (e.g.,
} cryostat slices) for distribution of the marker. I believe most fixatives
} containing Glutaraldehyde or formadehyde will quench the marker
} fluorescence and/or add autofluorescence. Any suggestions on how to
} prepare such tissue?
}
} Can we simply plungefreeze small pieces in LN or propane?
}
} Can we infiltrate the unfixed tissue first with sucrose as a
} cryoprotectant? The air pockets will no doubt cause additional problems.
} Would a quick exposure to a vacuum help the sucrose or fixative penetrate?
}
}
} This request is a new one for me. Any and all suggestions would be appreciated
}
} steve
}
} Dr. Steven Barlow
} EM Facility/Biology Dept.
} San Diego State University
} San Diego, CA 92182-4614
} phone: (619)594-4523
} fax:(619)594-5676
} email:sbarlow-at-sunstroke.sdsu.edu
}

Freshly prepared formaldehyde should not give you any autofluorescence or
quench the probe to any great extent. I have had good luck fixing probes
such as various fluo-dextrans, lectins, some membrane dyes, etc.

Lung is often a pain to cryo-section. I'd start with the easiest, a PF fixed
piece of tissue, freeze it in OCT and section.

Hope it works

Tom

Thomas Moninger (moninger-at-emiris.iaf.uiowa.edu)
Central Microscopy Research Facility
85 EMRB University of Iowa
Iowa City, IA 52242 319-335-8142 FAX 319-335-8049





From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Mon, 3 Jun 1996 11:39:46 -0700 (PDT)
Subject: Re: LM: pre-embedded fluorescent mar

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Steve,
The effect of fixation will depend upon the actual fluorophore employed.
There are several which survive formaldehyde fixation. Formaldehyde
autofluorescence hasn't been that big of an issue for us. Glut can be a
problem in the green/yellow emission range but not so bad in longer
wavelengths. If her particular needs dictate, you might try Carnoy's fluid
or a methanolic version. The shrinkage is considerable, but it penetrates
well and allows fairly good morphology. Hopefully, you have some
tissue for trials.

Good luck,

Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu




On Mon, 3 Jun 1996, Steve Barlow wrote:

} howdy all
}
} I have a user with lung tissue that has been labelled with a fluorescent
} marker in vivo. She wants to examine light microscope slices (e.g.,
} cryostat slices) for distribution of the marker. I believe most fixatives
} containing Glutaraldehyde or formadehyde will quench the marker
} fluorescence and/or add autofluorescence. Any suggestions on how to
} prepare such tissue?
}
} Can we simply plungefreeze small pieces in LN or propane?
}
} Can we infiltrate the unfixed tissue first with sucrose as a
} cryoprotectant? The air pockets will no doubt cause additional problems.
} Would a quick exposure to a vacuum help the sucrose or fixative penetrate?
}
}
} This request is a new one for me. Any and all suggestions would be appreciated
}
} steve
}
} Dr. Steven Barlow
} EM Facility/Biology Dept.
} San Diego State University
} San Diego, CA 92182-4614
} phone: (619)594-4523
} fax:(619)594-5676
} email:sbarlow-at-sunstroke.sdsu.edu
}
}
}






From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Mon, 3 Jun 1996 09:14:10 -0800
Subject: LM: pre-embedded fluorescent marker

Contents Retrieved from Microscopy Listserver Archives
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howdy all

I have a user with lung tissue that has been labelled with a fluorescent
marker in vivo. She wants to examine light microscope slices (e.g.,
cryostat slices) for distribution of the marker. I believe most
fixatives
containing Glutaraldehyde or formadehyde will quench the marker
fluorescence and/or add autofluorescence. Any suggestions on how to
prepare such tissue?

Can we simply plungefreeze small pieces in LN or propane?

Can we infiltrate the unfixed tissue first with sucrose as a
cryoprotectant? The air pockets will no doubt cause additional
problems.
Would a quick exposure to a vacuum help the sucrose or fixative
penetrate?


This request is a new one for me. Any and all suggestions would be
appreciated

steve

Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego, CA 92182-4614
phone: (619)594-4523
fax:(619)594-5676
email:sbarlow-at-sunstroke.sdsu.edu



We deal with lung tissue all the time in our lab. We inflate the lung
through the bronchus/trachea using 50% OCT (Cryomatrix) compound in
normal saline as a cryoprotectant. We attach a syringe filled with the
OCT to the trachea/bronchus and inject the stuff down the bronchial
tree. We then freeze the lung over liquid nitrogen (if you submerge the
lung in liquid nitrogen, the cryoprotectant expands and the lung
cracks). We store the samples in - 70 freezer and then section on a
cryostat. We fix the sections in acetone for 10 minutes prior to
staining. We sometimes have problems with autoflourescence but
depending on the wavelength they are using it can be done away with
(don't use fluorescein). If you need any other info please contact me.

Mark Elliott, PhD
UBC-Pulmonary Research Laboratory,
Vancouver BC
Canada





From: ROBIN CROSS :      EURC-at-giraffe.ru.ac.za
Date: Tue, 4 Jun 1996 07:43:47 GMT+0200
Subject: ink analysis

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Good morning all!

Does anyone know of a method for determining age-related
changes (years or tens of years, not hundreds or thousands of
years) in inks, i.e. compositional or any other changes?

Best regards on a bright but very chilly South African morning!


Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)




From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Tue, 4 Jun 1996 08:26:26 GMT
Subject: Re: Databases

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At 02:12 PM 6/3/96 -0400, you wrote:
} Hello all,
} We are interested in learning what kind of database people are finding
} most successful for billing purposes and workload recording. We are
} currently using a Paradox (DOS) which is networked between our imaging
} facility and business office. Our billing tends to be complicated since
} we have multiple users, budget numbers and equipment as well as multiple
} people inputting information and we are looking for an efficient way to
} streamline our operations. Any comments would be greatly appreciated,
} either to me directly or to the list.
}
} Regards,
} Marilyn
}
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
} + Marilyn Wadsworth | Phone: (802) 656-0813 +
} + Cell Imaging Facility | E-Mail: mwadswort-at-moose.uvm.edu +
} + School of Medicine, Path Dept | +
} + University of Vermont | +
} + Burlington, Vermont 05405 | +
} +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
}
}


There was a similar discussion on the list recently which
has been archived at the www address at the end of this message. Click on
"Tips & Tricks", find the link "computer applications" and click. You will
find the file there. If you do not have web access then let me know and I
will e-mail it to you




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/





From: SBDX78A-at-PRODIGY.COM ( KATY T RUSHNOV) (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Tue, 4 Jun 1996 08:15:09 -0500
Subject: used ultra wide 10x eyepieces

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Does anyone have a pair of uw 10x eyepieces for a Nikon microscope they
would like to sell? The part no. for them is 79046. My email address is
sbdx78a-at-prodigy.com. Thank you.







From: Jane A. Fagerland (847) 935-0104 :      FAGERLAND.JANE-at-igate.pprd.abbott.com
Date: Tue, 04 Jun 1996 09:28:00 -0500 (CDT)
Subject: LM:pre-embedding fluorescent marker

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Some thoughts on cryosectioning lungs.....The following procedure worked
very well for me when I was doing in situ hybridization in mouse lungs:

} Fix the lungs intratracheally with 4% paraformaldehyde in PBS, using
gravity feed
} Fix at 4C overnight
} Rinse in cold PBS, 3 times for 5-10 minutes each
} Immerse in 30% sucrose in cold PBS, overnight at 4C or until the
lungs sink
} Drain excess liquid and freeze in OCT or Lipshaw M1 (I embedded them
in histology molds complete with backing rings that fit in the cryostat,
then froze them in liquid nitrogen in a stainless steel tray held over a
styrofoam container of liquid nitrogen.) The Lipshaw medium was easier
for me to section than the OCT.

The morphology I got with this procedure was excellent - comparable to
that in paraffin-sections.

Good luck!

Jane A. Fagerland, Ph.D.
Dept. Microscopy and Microanalysis
Abbott Laboratories
Abbott Park IL 60064






From: Dr. Andrew P. Somlyo :      aps2n-at-elvis.med.virginia.edu
Date: Tue, 4 Jun 1996 10:50:44 -0400 (EDT)
Subject: Re: EDS System Selection (longish)

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Microscopy-at-Sparc5.Microscopy.Com

We are attempting to replace our ISIS system.

On Fri, 24 May 1996, Oxford Instruments Pty Ltd wrote:

} Dear Paul,
} In regard to your question, I would like to point out that the Oxford
} Instruments Link ISIS system has a fully quantitative mapping package
} (Quantmap). Full spectrum processing (including digital filtering and
} fitting) followed by a quantitative matrix correction is performed at each
} point. The resulting digital images show elemental concentrations rather
} than just a scaled x-ray intensity map. Standard deviation maps are also
} produced to allow significance testing.
}
} Your local Oxford rep would be delighted to tell you more!
} Best Regards, Julie Sheffield-Parker.
}
} At 05:15 PM 21/05/96 -0700, you wrote:
}
} }
} } Tell me this: who has an EDS system that can obtain a digital image of a
} } sample where a full least-squares fit and ZAF correction has been done on
} } the fly at each pixel, and the resulting image portrays the concentration
} } of the element mapped (not a scaled x-ray intensity map). I've seen papers
} } at national meetings describing this capability, as far back as 5-10 years
} } ago. The limitation for sure isn't the processing speed of the computers
} } now available, or the amount of RAM or hard drive storage space. How hard
} } can it be to do a complete analysis at each pixel in an image?
} }
} } Remember, our goal was to do quantitative EDS.
} }
} } Paul Carpenter
} }
} }
} }
} } +----------------------------------------------------+
} } | Paul K. Carpenter paulc-at-gps.caltech.edu |
} } | Division Analytical Facility |
} } | Geological and Planetary Sciences MC 170-25 |
} } | California Institute of Technology |
} } | Pasadena, CA 91125 |
} } | 818-395-6126 (X-ray Lab) 818-568-0935 (Dept. FAX) |
} } +----------------------------------------------------+
} }
} }
} }
} }
}
}
} *************************************************
} From:-
}
} Julie Sheffield-Parker,
} Oxford Instruments Pty. Ltd.,
} P. O. Box 7,
} Pennant Hills,
} NSW 2120,
} Sydney, AUSTRALIA
}
} Tel: ++ 61 2 484 6108
} Fax: ++ 61 2 484 1667
} E-Mail: oisydney-at-ozemail.com.au
}
} *************************************************
}
}




From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Wed, 5 Jun 1996 11:11:16 +1200
Subject: EM: Flourisol

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Has anyone heard of or/and used Flourisol? It is used fracturing samples
for SEM after dehydration, the sample is frozen and fractured in this
medium. I would like to know how to obtain it.

Thanks in advance.

Please reply to mark.gould-at-stonebow.otago.ac.nz

Mark Gould






From: bsgphy3-at-uconnvm.uconn.edu (JIM ROMANOW)
Date: Tue, 4 Jun 1996 15:20:45 -0500
Subject: Viton O-rings?

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Does anyone have a reliable source for Viton o-rings? I can not find a
distibutor who will accept orders for small quantities anymore. (5 to 10
rings per part number, less than a 50-100 dollar minimum order) and many of
the rings I have purchased recently seem to be of poorer quality than 10
years ago.
Thank you.


Jim Romanow
Electron Microscopy Facility
Physiology and Neurobiogy Department
The University Of Connecticut
Storrs
bsgphy3-at-uconnvm.uconn.edu
(860) 486-2914 voice
-1936 fax






From: Steven W. Miller :      73150.2217-at-CompuServe.COM
Date: 04 Jun 96 15:52:57 EDT
Subject: Ink and Toner Analysis

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The best analysis may not be public. The FBI and IRS (yes that is the Internal
Revenue Service) have extensive techniques for analysis of documents and inks.
They have every kind of analytical instrument you can imagine.
I would imagine most governments have similar labs. I have never tried to see
how much is published but I can't imagine it is in their best interest to
publish outside of forensic journals.

Maybe someone else can point you to a specific forensic journal or reference.

Good Luck,
Steve Miller
Integrated Microsystems, Inc.





From: Ralph S. DaCosta :      rdacosta-at-oci.utoronto.ca
Date: Mon, 03 Jun 1996 14:44:17 -0700
Subject: Absorption/Fluorescence of eosinophils.

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Message-Id: {31B35CB1.1FFE-at-oci.utoronto.ca}

Dear Microscopists,

I am looking for data on absorption and fluorescence spectra for human
eosinophils. Perhaps one of you can tell me that they just happen to
have such information sitting in a file somewhere....I hope. I have
already called several flow cytometry/hematology people (ie. Coulter,
etc), but unfortunately they do not have such info on hand. Curious, but
true.

Thanks and regards to all.


Ralph S. DaCosta
Department of Clinical Physics,
University of Toronto,
Ontario Cancer Institute,
Princess Margaret Hospital,
610 University Ave.,
Toronto, Ontario, Canada.




From: Marc C. Brande, MS, Founder (619) 587-4830 FAX: (619) 552-1516 :      mcbrande-at-sierra.net
Date: Tue, 04 Jun 1996 16:56:47 +0000
Subject: Cell Imaging: Phase + Fluorescence in 1 Video camera?

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Message-Id: {31B46ACF.5BCB-at-sierra.net}

Besides Optronics, are there other video cameras (digital/analog) that
will give equally good cultured cell images under both Phase Contrast
and Fluorescence? I know from experience that a Hamamatsu SIT that
does fine with low-level fluorescence does not image comparable to a
(ie.) Dage Newvicon under Phase Contrast. Is this too much to ask of a
single video camera?

Any comments will be greatly appreciated.

Marc




From: Douglas St. Denny :      saint-at-eastworld.net
Date: Wed, 05 Jun 1996 10:44:12 +0000
Subject: E. Leitz microscope

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Can anyone help me find the manufacturing date of an E. Leitz stereo
microscope, serial number 399230. It has three sets of eye pieces, and
three nose pieces. I would also be interested in finding a collectors
group, and knowing of any good books about collecting microscopes. Is
there a "Price Guide" that would let me know the market value or if
this microscope is even worthwhile as a collectible?

Thank you,
Douglas St. Denny,
Discovery Bay, Hong Kong

e mail - saint-at-eastworld.net




From: Rachel Teitelbaum :      teitelba-at-aecom.yu.edu
Date: Tue, 4 Jun 1996 16:06:23 -0400 (EDT)
Subject: Re: LM: pre-embedded fluorescent marker

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microscopy-at-sparc5.microscopy.com

}
} Freshly prepared formaldehyde should not give you any autofluorescence or
} quench the probe to any great extent. I have had good luck fixing probes
} such as various fluo-dextrans, lectins, some membrane dyes, etc.
}
} Lung is often a pain to cryo-section. I'd start with the easiest, a PF fixed
} piece of tissue, freeze it in OCT and section.
}


This may still interfere with the label though. A neat trick with lung
is to heat the oct in a syringe (5 cc syringe with a 26g needle) at 37
degrees, in an incubator, and while warm, inject into the trachea,
inflating the lung that way. Sectioning will be much better than if
immersed only in oct. You can then freeze, cryosection, label, and then
fix with acetone, and lose no antigenicity. Hope this helps.

-Rachel




From: Colin Veitch :      C.Veitch-at-geel.dwt.csiro.au
Date: Tue, 4 Jun 1996 18:36:26 -0500
Subject: Uranyl Acetate Saftey Data

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Hi all,

We are about to start using uranyl acetate for some staining applications
but have no MSDS information regarding the handling or disposal of it. We
can only get information for uranium products in general. The company which
produced it no longer produces it and the company they suggested may have
information no longer exists! A search of the WWW has also drawn a blank!

If anyone out there could email this information to me it would be greatly
appreciated as it is required with some urgency!

Many thanks in advance.

Colin Veitch

#####################################################################
# #
# Colin.Veitch-at-geel.dwt.csiro.au #
# Instrumentation Scientist #
# CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 #
# P.O. Box 21 Fax. +61 (0) 52 275657 #
# BELMONT Vic 3216 #
# Australia #
# #
# "We see the Universe the way it is because if it were different, #
# we would not be here to observe it." #
# #
#####################################################################





From: orion-at-infoboard.be (Jean Leclef)
Date: Wed, 5 Jun 1996 11:24:10 +0200 (MET DST)
Subject: Micro Lumina

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Hello all,

the Micro Lumina camera can be found at the following addresses (this is of
course not a limited list):

- in the US: contact ElectroImage NY at

- electroimg-at-aol.com
- microbill-at-aol.com
- fax 516 773 2955
- tel 516 773 4305

- in Belgium: contact E.L.I. sprl at

- orion-at-infoboard.be
- fax (32) 67 22 09 53
- tel (32) 67 21 25 07

- in Germany

- 100116.1420-at-compuserve.com
- Leica Vertrieb GmbH (fax (49) 6251-136-185)

- in France

- I.C.I. sarl Belfort (fax (33) 84 54 03 98 - tel (33) 84 58 02 43)


good luck - hope this will help you


John

orion-at-infoboard.be





From: BICH-at-PATD01.HS.SLL.SE (Birger Christensson)
Date: Wed, 5 Jun 1996 12:56:07 +0100
Subject: CCD camera

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Message-Id: {199606051148.AA14756-at-admd04.hs.sll.se}

We are in the process of setting up a system for fluoresent microscopy,
wide field, for deconvolution and 3-D analysis of signaltransduction
molecules.
I would very much appreciate suggestions in the choice of:
camera (is Sensys good enough? or alternatives to Photometrics),

filter/shutter wheel system,

Z-focus drive (accuracy/stability),

and deblurring/deconvolution algorithms.

Are there any evalations/comparisons of these components published or
otherways available.

All help much aprreciated

Birger

-------------------------------------------------------------------------
Birger Christensson, MD, PhD
Dept. of Pathology, F49
Huddinge University Hospital,
S-14186 Huddinge,
SWEDEN,
Tel +46-8-7461000
Fax +46-8-7795520
bich-at-PATD01.HS.SLL.SE





From: Colin Veitch :      C.Veitch-at-geel.dwt.csiro.au
Date: Tue, 4 Jun 1996 18:36:26 -0500
Subject: Uranyl Acetate Saftey Data

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Message-Id: {n1378161523.62956-at-QuickMail.Yale.edu}
"Colin Veitch" {C.Veitch-at-geel.dwt.csiro.au} ,
"Microscopy-at-Sparc5.Microscopy.Co" {Microscopy-at-Sparc5.Microscopy.Com}
X-Mailer: Mail*Link SMTP-QM 3.0.3 b1 d5

RE} Uranyl Acetate Saftey Data 6/5/96

Dear Colin,
I have recently purchased uranyl acetate from Polysciences, Inc. USA. Along
with my order I was given an MSDS sheet. I hope it is helpful, but please keep
in mind, these follow United States safety standards as well as universal
precautions. Cheers, Linda Iadarola.

Section I Identification: uranyl acetate (uranium oxyacetate) C4H6O6U.2H2O
Section II Hazardous Ingredients: depleted uranium at (0.00028mCi/g)
Section III Physical Data: Boiling point 527 (decomposes); Solubility in water;
appearance, yellow and solid; Melting Point 110C. None of the following
apply:vapor pressure, vapor density, % volatile by volume, evaporation rate,
specific gravity.
Section IV Explosion and fire hazard data: extinguishing media: water, carbon
dioxide, dry chemical powder, foam. Special fire fighting procedures:
firefighters must wear self-contained breathing aparatus and fully protective
equipment. No unusual fire and explosion hazards.
Section V Health hazards: Routes of entry: inhalation, skin and ingestion.
Extremely toxic by inhalation and ingestion. Danger of cumulative health
effects, may result in kidney damage. Emergency and First Aid procedures: skin
contact-wash affected area with copious amounts of water. eye contact-fluch eyes
with water for at least 15 minutes. inhalation-remove to fresh air. give oxygen
or artificial respiration as needed. ingestion-wash out mouth thoroughly and
induce vomiting. call physician.
Section VI Reactivity Data: stable, avoid heat, incompatible with oxidizing
agents, hazardous decomposition products include carbon monoxide and coarbon
dioxide
Section VII Spill or Leak Procedures: Steps to take if spills or leaks
occur-wear self-contained breathing aparatus, rubber boots and gloves. Sweep
up. Do not raise dust. Wash and ventilate spill site after pick up is complete.
Waste disposal method-bury in approved landfill according to federal, state and
local regulations.
Section VIII Special protection information: Use respiratory protection,
ventilate with local exhaust, wear rubber gloves and safety goggles, keep shower
and eye bath in local area.
Section IX Special precautions: Store material at room temperature. Keep
storage container tightly closed. Avoid exposure of food or drink to product.
Cover any cuts or skin abrasions. Wash thoroughly after handling.


--------------------------------------

We are about to start using uranyl acetate for some staining applications
but have no MSDS information regarding the handling or disposal of it. We
can only get information for uranium products in general. The company which
produced it no longer produces it and the company they suggested may have
information no longer exists! A search of the WWW has also drawn a blank!

If anyone out there could email this information to me it would be greatly
appreciated as it is required with some urgency!

Many thanks in advance.

Colin Veitch

#####################################################################
# #
# Colin.Veitch-at-geel.dwt.csiro.au #
# Instrumentation Scientist #
# CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 #
# P.O. Box 21 Fax. +61 (0) 52 275657 #
# BELMONT Vic 3216 #
# Australia #
# #
# "We see the Universe the way it is because if it were different, #
# we would not be here to observe it." #
# #
#####################################################################


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From: David S Leaf :      dslmap-at-honeydew.cc.wwu.edu
Date: Wed, 5 Jun 1996 08:21:58 -0700 (PDT)
Subject: unsubscribe

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please unsubscribe





From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Wed, 5 Jun 1996 11:26:25 -0400
Subject: SUBSCRIBE

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SUBSCRIBE





From: NANCY SMITH :      NSMITH-at-darwin.sci.csuhayward.edu
Date: Wed, 5 Jun 1996 09:23:03 PSD8PDT
Subject: uranyl acetate

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Colin Veitch requested information regarding uranyl acetate handling
and safety data. Could respondents please post their replies to
the list? The responses would be valuable to many of us.

Thank you
Nancy Crise Smith




From: James Thomas :      73772.3542-at-CompuServe.COM
Date: 05 Jun 96 14:08:32 EDT
Subject: Cheap Scopes

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Marcia,

Thanks for the info. We received it via our Cray XMP or maybe it was a PCXT.

RMM





From: Kurt.Albertine-at-hsc.utah.edu
Date: Wed, 05 Jun 1996 09:42 -0700 (MST)
Subject: TEMs for sale

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Message-Id: {199606051826.OAA17138-at-julian.uwo.ca}
To: microscopy {Microscopy-at-sparc5.microscopy.com}

Dear Nestor Zaluazac:
I am writing to you through Steve Kuzmic and Jonathan Krupp.
Jonathan provided some information about the MSA bulletin board
service. Would you please post the following message for me?

For sale: Two (2) JEOL 100S transmission electron microscopes:
$5,000 each. Both are in excellent condition; one is in mint
condition. Spare parts from a third JEOL 100S are also available at
no additional cost with purchase of one of the microscopes.
Relocation fee of each instrument will be $2,000 (Steve Kuzmic; S & J
Services). Contact Kurt H. Albertine, Ph.D., University of Utah, at
eMail address: Kurt.Albertine-at-hsc.utah.edu
(FAX 801-585-7395).

Thank you.

Kurt H. Albertine, Ph.D.
Director, Health Sciences Center Research Microscopy
Facility





















.




From: Liang, Long :      LLIANG-at-is.arco.com
Date: 05 Jun 1996 10:26:10 CST
Subject: EPMA: TN-5500 replacement ?

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Message-Id: {MACMS.LLIANG.863725140096157FMACMS-at-IS.ARCO.COM}

Dear Microscopists,

I have a JEOL 733 electron probe in my lab. The operations of the probe
are controlled by using TN-5500, TN-5600, and Task-5 WDS automation
program (written in Flextran language). The TN-5500 is an old system
which is costy for maintenance.

Does anyone know of any compatible (or better) PC system which can
replace the TN-5500 ?

Thanks in advance.

Long Liang
ARCO EPMA/SEM Lab
Plano, TX
lliang-at-is.arco.com






From: McPherson Family :      gangof4-at-sover.net
Date: Wed, 05 Jun 1996 18:26:02 -0700
Subject: job posting

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Message-ID: {31B633AA.3FB0-at-sover.net}

Omega Optical is looking for a qualified individual to fill the position
of Product Manager for fluorescence microscopy products. Specifically we
want someone who has strengths in micrscopy (hardware, software, optics,
chemistry, life sciences) combined with strong communication skills
(verbal and written).

If anyone knows of someone who would qualify and has an interest in such
a position, they can submit CV or resume to Human Resources via e-mail or
other methods.

John McPherson
omega-at-sover.net

Omega Optical
Box 573
3 Grove Street
Brattleboro, VT 05301




From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 5 Jun 1996 13:00:43 -0400 (EDT)
Subject: Re: Viton O-rings?

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}
} Does anyone have a reliable source for Viton o-rings? I can not find a
} distibutor who will accept orders for small quantities anymore. (5 to 10
} rings per part number, less than a 50-100 dollar minimum order) and many of
} the rings I have purchased recently seem to be of poorer quality than 10
} years ago.

Dear Jim,
Our last order (for 5-10 of each of 4 sizes, and totaling } $100)
was from Web Seal Inc., 206 Marcellus St., Syracuse NY 13204, phone #
(315) 475-8496. I believe they also carry other elastomers, such as
polypropylene, which is the most radiation-resistant of the usual materials.
The quality seems to be the same as in the past, judging from how they are
when we remove them from the column for our annual cleaning.
Yours,
Bill Tivol




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 5 Jun 1996 14:26:44 -0400
Subject: RE-Viton O-ring Source

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Message-ID: {n1378137610.84139-at-mse.engin.umich.edu}

Subject: Time: 2:15 PM
OFFICE MEMO RE:Viton O-ring Source Date: 6/5/96

The Zatkoff Company in Detroit Michigan will sell O-rings in small lots, and
they stock O-rings in a very large range of sizes and materials. I just
picked up an assortment of Viton and Teflon O-rings from them yesterday.
Their addresss is:
Zatkoff Seals
23230 Industrial Park Drive
Farmington Hills, MI 48335-2850
Ph: 810-478-2400 Fx:810-478-3392
Their offices are mainly in the midwest; however, I am sure that if you were
to call in an order they would be willing to ship to any part of the country.
Good luck, W. C. Bigelow (bigelow-at-umich.edu)





From: Elinor Solit :      cambrex-at-world.std.com
Date: Wed, 5 Jun 1996 11:13:46 -0400 (EDT)
Subject: Re: E. Leitz microscope

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Hi Douglas,

The person who can help you to date the Leitz microscope may be Jan Hinsch.
He works in the Leica New Jersey branch. The number there is
201-767-1100, and the Fax is 201-767-4196. If you'd like to write him,
the address is 24 Link Drive, Rockleigh, NJ 07647.
Jan may also know of the collectors group or the market source that you seek.
Good luck to you, and my regards to Mr. Hinsch.

Elinor Solit
The Microscope Book

On Wed, 5 Jun 1996, Douglas St. Denny wrote:

} Can anyone help me find the manufacturing date of an E. Leitz stereo
} microscope, serial number 399230. It has three sets of eye pieces, and
} three nose pieces. I would also be interested in finding a collectors
} group, and knowing of any good books about collecting microscopes. Is
} there a "Price Guide" that would let me know the market value or if
} this microscope is even worthwhile as a collectible?
}
} Thank you,
} Douglas St. Denny,
} Discovery Bay, Hong Kong
}
} e mail - saint-at-eastworld.net
}




From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 5 Jun 1996 18:01:24 -0500
Subject: Oil Immersion

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Message-ID: {n1378124556.73211-at-msmail.tmc.tulane.edu}
"NIHImageUsers" {nih-image-at-soils.umn.edu}
X-Mailer: Mail*Link SMTP-MS 3.0.2

I am sudenly having reaction to two different oil immersion we have. Please
send source for supplier of different types of oil immersion of high quality.
Is the Cargille Lab still in business?

Send response to me at address below. Gracias.

*********************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} ----} Prof. Pathology & Otolaryngology *
*http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html*
*********************************************************************




From: scott.wight-at-nist.gov (Scott Wight)
Date: Wed, 5 Jun 1996 15:49:31 -0500
Subject: Re: Uranyl Acetate Saftey Data

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Colin and fellow netters:
The best place to get information of this type is on the SAFETY listserver,
it has as many safety professionals as this list has microscopists.
Directions to send a message and/or join the list are below:
------------------------------------------------------------------------------
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} Hi all,
}
} We are about to start using uranyl acetate for some staining applications
} but have no MSDS information regarding the handling or disposal of it. We
} can only get information for uranium products in general. The company which
} produced it no longer produces it and the company they suggested may have
} information no longer exists! A search of the WWW has also drawn a blank!
}
} If anyone out there could email this information to me it would be greatly
} appreciated as it is required with some urgency!
}
} Many thanks in advance.
}
} Colin Veitch
}
} #####################################################################
} # #
} # Colin.Veitch-at-geel.dwt.csiro.au #
} # Instrumentation Scientist #
} # CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 #
} # P.O. Box 21 Fax. +61 (0) 52 275657 #
} # BELMONT Vic 3216 #
} # Australia #
} # #
} # "We see the Universe the way it is because if it were different, #
} # we would not be here to observe it." #
} # #
} #####################################################################

Scott Wight e-mail: SCOTT.WIGHT-at-NIST.GOV
NIST - Microanalysis Group W voice: 301-975-3949
Bld 222, Rm A113 | fax: 301-216-1134 or 301-417-1321
Gaithersburg, MD 20899 \|/ disclaimer:
Any opinion expressed is my own and does not represent those of my employer.






From: Probing & Structure :      pns-at-ultra.net.au
Date: Thu, 6 Jun 1996 09:43:19 +1000
Subject: Uranyl Acetate Safety Data

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you wrote:
Hi all,

We are about to start using uranyl acetate for some staining applications
but have no MSDS information regarding the handling or disposal of it. We
can only get information for uranium products in general. The company which
produced it no longer produces it and the company they suggested may have
information no longer exists! A search of the WWW has also drawn a blank!

If anyone out there could email this information to me it would be greatly
appreciated as it is required with some urgency!

Many thanks in advance.

Colin Veitch

#####################################################################
# #
# Colin.Veitch-at-geel.dwt.csiro.au #
# Instrumentation Scientist #
# CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 #
# P.O. Box 21 Fax. +61 (0) 52 275657 #
# BELMONT Vic 3216 #
# Australia #
# #
# "We see the Universe the way it is because if it were different, #
# we would not be here to observe it." #
# #
#####################################################################

Colin and whoever is looking for those Material Safety Data Sheets:

A great many of these are available at these two sites

University of Utah gopher://gopher.chem.utah.edu:70/11/MSDS

North West Fisheries http://research.nwfsc.noaa.gov/msds.html

In Australia some "wise guy" decided to require the facts to be set out in a
different order. They do though allow the European Community MSDS but the
American MSDS form is not acceptable here. Since several hundred thousand
chemicals are imported this is a big job and hugely expensive. We have
converted most of the EM chemical MSDS and they are available at our site.

Regards Jim Darley
Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
Internet Catalogue: http://www.ultra.net/~pns/





From: Colin Veitch :      C.Veitch-at-geel.dwt.csiro.au
Date: Wed, 5 Jun 1996 21:19:17 -0500
Subject: Thanks for Uranyl Acetate infromation

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Hi all,

Just a note to say thanks for all the replies regarding my question about
Uranyl Acetate.

It seems that the issue of disposal is not yet resolved but some clues were
gleaned from the responses.

Some of the MSDS's were faxed but all the email responses have been compiled
into the one (large) file. Rather than send this to the reflector, if you
would like a copy of the file (in ASCII text or Word 6 for Windows - please
specify) I'll email a copy to you!

Thanks again,

Colin Veitch
#####################################################################
# #
# Colin.Veitch-at-geel.dwt.csiro.au #
# Instrumentation Scientist #
# CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 #
# P.O. Box 21 Fax. +61 (0) 52 275657 #
# BELMONT Vic 3216 #
# Australia #
# #
# "We see the Universe the way it is because if it were different, #
# we would not be here to observe it." #
# #
#####################################################################





From: tania-at-dynamotive.com (Tania Jones)
Date: Wed, 05 Jun 1996 13:13:12 -0700
Subject: EM/EDX: finding thin hydrocarbon coatings

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Message-Id: {m0uROuc-0002VYC-at-dewey.mindlink.net}
X-Sender: tania-at-pop.mindlink.net
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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

hello,

our lab is trying to find a way to detect very thin ( {1um thick) coatings of
stearates on the surface of a galvanized wire. our equipment consists of only
a sem and a standard edx (no light element). any suggestions on how to "see"
the coating would be very helpful.

thank you in advance,

tania jones
laboratory manager
dynamotive technologies corp.





From: deborah Lietz :      dlietz-at-trentu.ca
Date: Thu, 06 Jun 1996 10:40:03 +0100
Subject: teaching videos

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Does anyone know of any videos which specifically address block trimming and
sectioning or any other microscopy video catalog other than the one put out by
the MSA?











From: deborah Lietz :      dlietz-at-trentu.ca
Date: Thu, 06 Jun 1996 10:39:11 +0100
Subject: teaching videos

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Does anyone know of any videos which specifically address block trimming and
sectioning or any other microscopy video catalog other than the one put out by
the MSA?











From: John Gabrovsek :      gabrovj-at-cesmtp.ccf.org
Date: Thu, 06 Jun 1996 11:23:16 -0400
Subject: TEM em-markers,

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Message-Id: {s1b6c059.085-at-cesmtp.ccf.org}
X-Mailer: Novell GroupWise 4.1

I would like to thank to all who responded to my question:"How to
visualize injected plastic microspheres which migrate through the
arterial wall in TEM preparation?" I got many different suggestions.
Very interesting. Thanks again. Listserver is great communication
medium.
Regards,
John Gabrovsek
CCF Cleveland, Ohio





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 6 Jun 1996 12:55:23 -0400
Subject: RE- Cargille Labs

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Message-ID: {n1378056707.48923-at-mse.engin.umich.edu}

Subject: Time: 12:48 PM
OFFICE MEMO RE: Cargille Labs Date: 6/6/96

According to the latest directory of manufacturers published by R & D
Magazine the address for Cargille Laboratories is 55 Commerce Rd., Cedar
Grove, NJ 07009; Ph: 201-239-6633; Fx: 201-239-6096
Good lick, Wil Bigelow (Bigelow-at-umich.edu)





From: KLEABONAS.MATTHEW_P+-at-ALBANY.VA.GOV
Date: 06 Jun 96 10:51 EDT
Subject: SEM-HISTO SECTIONS

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HI MICRO-FANS,
I AM HAVING A BIT OF A PROBLEM SECURING WAX SECTIONS TO CARBON
STUBS. AFTER DEPARIFFINIZATIN AND CONDUCTIVE COATING THE SECTIONS
HAVE A TENDENCY TO LIFT OR CURL. IF ANY ONE HAS HAD ANY EXPERIENCE
WITH PARRAFIN SECTIONS FOR SEM THE INFO WOULD BE GREATLY APPRECIATED
THANKS,
MATT KLEABONAS
STRATTON VA MEDICAL CENTER
ALBANY,NY
TEL: (518)-462-3311 X2552
FAX: (518)-462-1258
E-MAIL: KLEABONAS.MATTHEW_P+-at-ALBANY.VA.GOV




From: Elinor Solit :      cambrex-at-world.std.com
Date: Thu, 6 Jun 1996 12:34:22 -0400 (EDT)
Subject: Re: EM/EDX: finding thin hydrocarbon coatings

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Tania,

Try cross-sectioning the material. If you have a cryostat all the better.

If you have difficulty, it will probably come from a need to support the
sample. Then it becomes a case of trying to find the best material.
Sometimes a fairly stiff plastic can help. Just be sure you know what
layer you're looking at in the microscope.

Good luck,

Elinor Solit
The Microscope Book

On Wed, 5 Jun 1996, Tania Jones wrote:

} hello,
}
} our lab is trying to find a way to detect very thin ( {1um thick) coatings of
} stearates on the surface of a galvanized wire. our equipment consists of only
} a sem and a standard edx (no light element). any suggestions on how to "see"
} the coating would be very helpful.
}
} thank you in advance,
}
} tania jones
} laboratory manager
} dynamotive technologies corp.
}




From: Joe D Geller :      geller-at-world.std.com
Date: Thu, 6 Jun 1996 10:04:57 -0400 (EDT)
Subject: Re: EM/EDX: finding thin hydrocarbon coatings

Contents Retrieved from Microscopy Listserver Archives
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On Wed, 5 Jun 1996, Tania Jones wrote:

} hello,
}
} our lab is trying to find a way to detect very thin ( {1um thick) coatings of
} stearates on the surface of a galvanized wire. our equipment consists of only
} a sem and a standard edx (no light element). any suggestions on how to "see"
} the coating would be very helpful.
}
} thank you in advance,
}
} tania jones
} laboratory manager
} dynamotive technologies corp.
}
I would be concerned about electron stimulated desorption of the stearate
coating on the wire. A good way to analyze this would be to use ESCA
(XPS) Electron Spectroscopy for Chemical Analysis (X-ray Photoelectron
Spectroscopy). This non-ionizing technique uses x-rays for excitation and
detects the emitted photoelectrons. By analyzing the electron binding
energy one can determine the near (5nm) surface composition of the wire.

Joe Geller
Geller Microanalytical Lab
jg-at-gellermicro.com




From: Joe D Geller :      geller-at-world.std.com
Date: Thu, 6 Jun 1996 09:54:21 -0400 (EDT)
Subject: Re: EPMA : standards for Rb and Cs

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On Wed, 5 Jun 1996, Yves Thibault wrote:

} -- [ From: Yves Thibault * EMC.Ver #2.5.02 ] --
}
} Hi to all,
}
} I am planning to analyze with an elctron probe microanalyzer a series of
} synthetic alkali-rich silicate-phosphate glasses. Some will be made with Cs
} and others with Rb. I do not have proper standards for these two elements
} (Cs,Rb). I was wondering if someone would know a good source for such
} standards.
}
} Thank you,
}
} Yves Thibault
} Dept of Earth Sciences
} University of Western Ontario
} London, Ontario, CANADA, N6A 5L9
}
} e-mail ythibaul-at-julian.uwo.ca
}

We have a CsI standard and can prepare (as a custom standard) RbI. These
compounds are stable and behave well under electron beam irradiation.
Considering the accuracy of current ZAF programs, such as CITZAF, these
should be satisfactory standards for analyzing oxides of Cs and Rb.

Please note that we offer standards for EPMA as a normal part of our
business.

Joe Geller
Geller MicroAnalytical Laboratory
jg-at-gellermicro.com
508 887-7000




From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 6 Jun 1996 15:55:32 -0500
Subject: Got it-Oil-immer

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Message-ID: {n1378045728.17528-at-msmail.tmc.tulane.edu}
"NIHImageUsers" {nih-image-at-soils.umn.edu}
X-Mailer: Mail*Link SMTP-MS 3.0.2

Thanks for the prompt response. I now know that Cargille is still in business
and dozen of suppliers who sell their oil. I still do not know if there are
out there non allergenic immersion oils. Meanwhile I will just have to use
glove with oil immersion.




From: DChernoff-at-aol.com
Date: Thu, 6 Jun 1996 17:06:11 -0400
Subject: Electron Flight Simulator

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Keith,

I was sent a reply to your initial request from Veronique Buschmann. I wanted
to correct an error in the e-mail address and the web site. The correct
e-mail address is:
dchernoff-at-aol.com

The correct web site is:

http://members.aol.com/smworld100/index.htm

Please contact me if you have any questions about the Electron Flight
Simulator program.

Best Regards
Don Chernoff
Small World





From: Joe D Geller :      geller-at-world.std.com
Date: Thu, 6 Jun 1996 09:59:34 -0400 (EDT)
Subject: Re: EPMA: TN-5500 replacement ?

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On 5 Jun 1996, Liang, Long wrote:

} Dear Microscopists,
}
} I have a JEOL 733 electron probe in my lab. The operations of the probe
} are controlled by using TN-5500, TN-5600, and Task-5 WDS automation
} program (written in Flextran language). The TN-5500 is an old system
} which is costy for maintenance.
}
} Does anyone know of any compatible (or better) PC system which can
} replace the TN-5500 ?
}
} Thanks in advance.
}
} Long Liang
} ARCO EPMA/SEM Lab
} Plano, TX
} lliang-at-is.arco.com
}
}
We manufacture a replacement system for the TN-5500, 5600 and TASK WDS
automation, including the energy dispersive x-ray analyzer. For the EDS
we supply replacement HV and bias supply units that is not Nim Bin and a PC
based pulse height analyzer with qualitative and quantitative software
integrated with the WDS. Part of the package includes optional digital
imaging. We do have demonstraton disks available.

Joe Geller
jg-at-gellermicro.com
Geller MicroAnalytical Lab
426e Boston St.
Topsfield, MA 01983
508 887-7000, fax 508 887-6671




From: deborah Lietz :      dlietz-at-trentu.ca
Date: Thu, 06 Jun 1996 10:18:00 +0100
Subject: video equipment

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We are in the midst of purchasing some digital equipment for our microscopy lab.
If you are a regular to the forum you may remember being asked for some
suggestions. Well now we are getting to the task of sorting through all the
files we have gathered. I'm looking for opinions of those of you working with
equipment in the field who have no vested interest in any specific companies.
Please give positive and negative drawbacks and try to address the following
questions:

1. Does anyone have equipment hooked up to a cambridge S90 and if so what do
you have?
2. Are you currently using semicaps (Genie or 1000)?and give opinion of system
3. Other than semicaps is there any other company that uses active beam control
(ie controls electron beam)to maximize resolution?
4. Is your system user friendly and geered for multiple users?
5. Is your system Mac or not?

Just some background information:
We have an electron microscopy class of 24 students and we are trying to expose
them to the art of digital archiving as well as aleviate some time spent in the
darkroom. We also in our biology department have 10 computer stations which are
on our network.(Macs). The system is going to be used for capturing images from
the sem,lm and copystand , and tem. These images will be labeled etc. and down
loaded through the network for tutorials on the computers. Images will be
supported in colour and can also be used for manuals and manuscripts.

Thanks,

sincerely Debbie Lietz
Electron Microscopy Suite
Department of Biology
Trent University
Peterborough, Ontario
K9J 7B8
email DLietz-at-trentu.ca





From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Thu, 6 Jun 1996 16:11:08 -0500
Subject: Electronic booking etc

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This thread may be a little old, but we have had a system for
this for many years and it is way better than paper. Ours is not
commercial, and I quake at giving it to anyone else (it works, but it
is a mess). I found out today that a system which has been installed
on a couple of other microscopes here is being sold commercialy, at a
rather low (I think) price. If you are interested, contact Richard
Benassi at rbenassi-at-mcs.com . Before you ask, I have not commercial
interest in this, neither does Northwestern University.




From: deborah Lietz :      dlietz-at-trentu.ca
Date: Thu, 06 Jun 1996 10:20:52 +0100
Subject: video equipment for microscopy

Contents Retrieved from Microscopy Listserver Archives
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We are in the midst of purchasing some digital equipment for our microscopy lab.
If you are a regular to the forum you may remember being asked for some
suggestions. Well now we are getting to the task of sorting through all the
files we have gathered. I'm looking for opinions of those of you working with
equipment in the field who have no vested interest in any specific companies.
Please give positive and negative drawbacks and try to address the following
questions:

1. Does anyone have equipment hooked up to a cambridge S90 and if so what do
you have?
2. Are you currently using semicaps (Genie or 1000)?and give opinion of system
3. Other than semicaps is there any other company that uses active beam control
(ie controls electron beam)to maximize resolution?
4. Is your system user friendly and geered for multiple users?
5. Is your system Mac or not?

Just some background information:
We have an electron microscopy class of 24 students and we are trying to expose
them to the art of digital archiving as well as aleviate some time spent in the
darkroom. We also in our biology department have 10 computer stations which are
on our network.(Macs). The system is going to be used for capturing images from
the sem,lm and copystand , and tem. These images will be labeled etc. and down
loaded through the network for tutorials on the computers. Images will be
supported in colour and can also be used for manuals and manuscripts.

Thanks,

sincerely Debbie Lietz
Electron Microscopy Suite
Department of Biology
Trent University
Peterborough, Ontario
K9J 7B8
email DLietz-at-trentu.ca





From: Ian Hall :      hall-at-me.udel.edu
Date: Thu, 6 Jun 1996 09:43:26 -0400 (EDT)
Subject: Electropolishing Mg??

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Colleagues
Working temporarily far from my books and reference manuals, I
need to make thin foils of a magnesium alloy. Any helpful suggestions
from experienced TEM thin foil preparers on the following points would
be very welcome indeed.

Electrolyte:
Voltage:
Temperature
Special handling, foil rinsing, storage etc...
(Material: Mg-9Al-1Zn, die cast. Available equipment: Fischione
twin-jet.)

Thanks in anticipation for any help.
Rick Hall
Materials Science, Univ. of Delaware
(presently at:
Technion, Israel Institute of Technology)






From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Thu, 6 Jun 1996 16:16:16 -0500
Subject: Re: SEM-HISTO SECTIONS

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} HI MICRO-FANS,
} I AM HAVING A BIT OF A PROBLEM SECURING WAX SECTIONS TO CARBON
} STUBS. AFTER DEPARIFFINIZATIN AND CONDUCTIVE COATING THE SECTIONS
} HAVE A TENDENCY TO LIFT OR CURL. IF ANY ONE HAS HAD ANY EXPERIENCE
} WITH PARRAFIN SECTIONS FOR SEM THE INFO WOULD BE GREATLY APPRECIATED
} THANKS,
} MATT KLEABONAS

Try poly-l-lysine or chromating the stubs--chrome albumin or chrome
gelatin, as is done for slides.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
soon-to-be-closed
Center for Electron Microscopy
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********






From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Thu, 6 Jun 1996 14:46:17 -0400 (EDT)
Subject: Re: SEM HISTOSECTIONS

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On Thu, 6 Jun 1996 KLEABONAS.MATTHEW_P+-at-ALBANY.VA.GOV wrote:

} HI MICRO-FANS,
} ATTEMPTS TO SECURE WAX SECTIONS FOR SEM-EDXA HAVE BEEN SOMEWHAT
} UNSUCCESFUL. AFTER CONDUCTIVE COATING SECTIONS CURL AND SOME-
} TIMES PARTLY DETACH. TECH INFO WOULD BE GREATLY APPRECIATED.
} MATT KLEABONAS
} STRATTON VAMC
} ALBANY,NY
} TEL: (518)-462-3311 X2552
} E-MAIL KLEABONAS.MATTHEW_P+-at-ALBANY.VA.GOV
}
Matt:
I had to do the same sort of thing on paraffin sections and I had our
histologist cut the sections and place the sections on carbon planchets.
I then deparaffinized the sections while they were still on the planchet.
I looked at them uncoated in the SEM and did EDAX. I usd different tissues
such as kidney, heart, brain, etc. I was looking for silicon particles
coming from the tubing during cardio-pulmonary bypass operations. The
sections seemed to adhere to the carbon planchets. Don't know if this
will help or not with your applications, but you can give it a try.

Peace,

Phil




From: deborah Lietz :      dlietz-at-trentu.ca
Date: Thu, 06 Jun 1996 10:21:05 +0100
Subject: video equipment for microscopy

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We are in the midst of purchasing some digital equipment for our microscopy lab.
If you are a regular to the forum you may remember being asked for some
suggestions. Well now we are getting to the task of sorting through all the
files we have gathered. I'm looking for opinions of those of you working with
equipment in the field who have no vested interest in any specific companies.
Please give positive and negative drawbacks and try to address the following
questions:

1. Does anyone have equipment hooked up to a cambridge S90 and if so what do
you have?
2. Are you currently using semicaps (Genie or 1000)?and give opinion of system
3. Other than semicaps is there any other company that uses active beam control
(ie controls electron beam)to maximize resolution?
4. Is your system user friendly and geered for multiple users?
5. Is your system Mac or not?

Just some background information:
We have an electron microscopy class of 24 students and we are trying to expose
them to the art of digital archiving as well as aleviate some time spent in the
darkroom. We also in our biology department have 10 computer stations which are
on our network.(Macs). The system is going to be used for capturing images from
the sem,lm and copystand , and tem. These images will be labeled etc. and down
loaded through the network for tutorials on the computers. Images will be
supported in colour and can also be used for manuals and manuscripts.

Thanks,

sincerely Debbie Lietz
Electron Microscopy Suite
Department of Biology
Trent University
Peterborough, Ontario
K9J 7B8
email DLietz-at-trentu.ca





From: Elinor Solit :      cambrex-at-world.std.com
Date: Thu, 6 Jun 1996 12:18:14 -0400 (EDT)
Subject: Re: Oil Immersion

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Dr. Fermin,

Cargille is going strong. Call them at 201-239-6633, ask for Jean Behlen
or Oscar Sceen.

If I can offer a non-medical opinion, you may be having an allergic
reaction to the immersion oil. Allergies seem to become more frequent
with passing time. Maybe the result of ever-present toxins in our
environment. But as one who needs medicine to deal with a mosquito bite,
I can offer you sympathy.

Hope this helps.

Elinor Solit, The Cambrex Group
Publishers of The Microscope Book


On 5 Jun 1996, Fermin, Cesar wrote:

} I am sudenly having reaction to two different oil immersion we have. Please
} send source for supplier of different types of oil immersion of high quality.
} Is the Cargille Lab still in business?
}
} Send response to me at address below. Gracias.
}
} *********************************************************************
} *Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
} *Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
} *Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
} *New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
} * {Fermin-at-tmc.tulane.edu} ----} Prof. Pathology & Otolaryngology *
} *http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html*
} *********************************************************************
}




From: Ilene Sugino :      suginoik-at-UMDNJ.EDU
Date: Thu, 6 Jun 1996 16:11:40 -0400 (EDT)
Subject: Embedding and Sectioning help

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Message-Id: {199606062008.PAA02814-at-Sparc5.Microscopy.Com}

Medjet,Inc. located in Edison, NJ is seeking someone in a facility
(hopefully closeby) who can embed rabbit cornea and section it.
Embedding can be either in JB4 or Epon. They need samples prepared,
sectioned and photographed ASAP. It's a total of 8 tissue pieces.
Medjet will be offering monetary compensation. Please contact Dr. Peretz
Feder if you can be of help at 908 635-6604 or e mail him at
PFeder-at-aol.com.

Thanks.--



------------------------------------------------------------------------------

Ilene Sugino e-mail: suginoik-at-umdnj.edu
UMDNJ-Ophthalmology phone: (201) 982-7746
DOC 6th Floor fax: (201) 982-7762
90 Bergen Street
Newark, New Jersey 07103

------------------------------------------------------------------------------




From: KLEABONAS.MATTHEW_P+-at-ALBANY.VA.GOV (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Thu, 6 Jun 1996 08:26:21 -0500
Subject: SEM HISTOSECTIONS

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HI MICRO-FANS,
ATTEMPTS TO SECURE WAX SECTIONS FOR SEM-EDXA HAVE BEEN SOMEWHAT
UNSUCCESFUL. AFTER CONDUCTIVE COATING SECTIONS CURL AND SOME-
TIMES PARTLY DETACH. TECH INFO WOULD BE GREATLY APPRECIATED.
MATT KLEABONAS
STRATTON VAMC
ALBANY,NY
TEL: (518)-462-3311 X2552
E-MAIL KLEABONAS.MATTHEW_P+-at-ALBANY.VA.GOV







From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Thu, 6 Jun 1996 13:25:44 -0500
Subject: uranyl acetate saftey

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In response to the recent few messages on the hazards of uranium compounds used
in the EM lab, I'll pass on a few quotes from the article "Potential hazards of
uranium and its compounds in electron microscopy: a brief review", by James J.
Darley and Hisanori Ezoe, that appeared in the Journal of Microscopy, Vol. 106,
Pt 1, January 1976, pp 85-86, and save you the trouble of going to the library
to dig up the article. I also wish to question the use of 'depleted' on labels.
What caught my attention in this article is the stress put on the chemical
toxicity of uranium; aside from its radioactivity, its also a nasty poison. Here
are some quotes:

1. "The American Conference of Governmental Industrial Hygienists has set
standards based on chemical toxicity. The maximum daily intake of uranium is set
at under 2 micrograms. Fifty milligrams is considered a lethal dose. The maximum
allowable concentration in the air is 0.5 mg for arsenic and only 0.05 mg for
soluble uranium compounds per cubic metre of air. Acute poisoning is more likely
with uranium compounds which are soluble in body fluids. Injury to the body is
general, with the kidneys most affected..............Chronic poisoning is more
likely from long term, low doses of insoluble compounds..............Insoluble
compounds are more likely to lead to lung cancer. (Encyclopedia of Occupational
Health and Safety, 1972)..............Chemical toxicity outweighs radiological
toxicity of natural uranium."

2. "Touching, inhalation and ingestion of uranium compounds must be avoided.
Particular care must be used when dealing with the powdered
substances.............Evaporating uranium for shadowing should only be carried
out in an evaporator vented into a fume hood."

In consideration of the two points above I always work in the hood with the door
pulled down a bit when removing powdered uranium compounds from their bottles
for mixing solutions (same goes for sodium cacodylate which contains arsenic,
and for lead salts, etc.).

Of course we should not overlook the radioactive hazard posed by uranium
compounds. Here are some quotes from the authors about that:

3. "Chemical toxicity outweighs radiological toxicity of natural uranium.
However, it seems important to recognize that natural uranium compounds
constitute a substantial source of ionizing radiation..............Natural
uranium contains 99.28% U238, 0.714% U235 and 0.00548%
U234................Depleted uranium contains between 0.7% and usually more that
0.3% U235.

4. "One gram of natural uranium emits 12,500 decays/s of alpha particles,
25,000/s of beta emission, and also releases some gamma
radiation.....................We found the beta emission has sufficient energy
to penetrate glass and blacken a photographic film after a day's exposure to a
jar of uranyl acetate. The radiological toxicity of 100 g of uranyl acetate is
similar to thirty vials of C14 containing 20 microcuries each, with allowance
for energy and other modifying factors. The radiation hazard in a laboratory
considering 100 g of a uranium compound is in the same range as the average
biology laboratory using isotopes like H3, C14, P32, for tracer
experiments....................it appears important for the user of uranium
compounds to be aware of these facts."

The authors go on to bemoan the lack of hazardous substance warnings on labels
of "repacked materials from suppliers", but they wrote that 20 years ago and
today most EM uranium compound labels that I have seen are generally adequately
labeled as to the radioactive and toxic nature of contents. Uranyl magnesium
acetate from Polysciences, Inc., carries a "poison" warning (with skull &
crossbones) -but carries no warning as to the radioactivity of that compound(!).
Uranyl acetate from Ted Pella, Inc., carries a 'radioactive' and 'poison'
warning on its bottle, states the 0.51 microcurie activity level but does not
carry the 'depleted' label. Electron Microscopy Sciences, has a radioactive
warning sticker on its bottle of uranyl acetate, says it contains "U (depleted)
activity 0.51 microcuries/gm/s", has no poison warning, but they do include the
MSDS sheet (which also warns about carcinogenic effects of exposure) plus their
own 2 page handout on the radioactive properties of UA and an explanation of
units used to express radioactivity levels, including the definition of
"depleted" as quoted by the authors in 3 above.

The use of the word 'depleted' on uranium compound labels bothers me a little
because it implies that there is NO radioactivity left in it. My American
Heritage Dictionary defines 'deplete' as "to use up or exhaust, to empty." Thus
'depleted' would mean "used up, or exhausted, emptied," and clearly with respect
to UA 'depleted' means only about 50% reduction in what is already a minor
constituent of the uranium in UA, of U235 to "between 0.7% and usually more that
0.3%", as in quote 3 above. Even though the radioactivity level of 0.51
microcuries is stated, somehow the label 'depleted' implies that it is safer
somehow, but to me there is no way to clean up uranium's image as a nasty
substance. The 'depleted' label could be misleading to those not in the know
about what it means here. The Electron Microscopy Sciences' handout ends with a
good description of the actual situation inside that bottle: "Both natural and
depleted uranium, being a mixture of isotopes and daughters, will be expected to
demonstrate alpha, beta and gamma activity." That's why its labeled
"radioactive', but personally I think the "depleted" label is misleading.

Well, all this is to answer the original question on the dangers of uranium
compounds and why it should be handled carefully like any hazardous compound,
and to question the use of "depleted" on labels. The bottom line is that its 1.
a toxic poison, and 2. its radioactive. Use with the usual precautions.


Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: Charles A. Garber :      103532.3325-at-CompuServe.COM
Date: 06 Jun 96 09:18:06 EDT
Subject: Cs and Rb

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On June 7, Yves Thibault wrote:
========================================
I am planning to analyze with an elctron probe microanalyzer a series of
synthetic alkali-rich silicate-phosphate glasses. Some will be made with Cs and
others with Rb. I do not have proper standards for these two elements (Cs,Rb).
I was wondering if someone would know a good source for such standards.
===============================================

You can find in the SPI "53 minerals mount" the mineral "pollucite". It is
CsSi2AlO6. The analysis shows 30.0% Cs and 0.7%Rb as well as 1.3%Na and 0.1%K.
In other words - all the alkali metals but only Cs in any reasonable amount. I
doubt if it would be very much good as a standard for a major amount of Rb. The
alkali metals are too reactive to mount as metals, which is the reason they are
not in the SPI Supplies "44 Metals" mount.

More information about these standards for microanalysis and prices can be found
on our web site given below.

Two other points: a) The "homogeneity" of this mineral is considered
outstanding as determined both by ourselves and our customers, and b) we don't
normally offer the mineral by itself, however I guess our arms could be twisted
if that really was the only mineral you wanted (needed).

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.com

####################################
WWW: http://www.2spi.com
####################################
======================================================





From: Veronique Buschmann :      bushman-at-ruca.ua.ac.be
Date: Thu, 6 Jun 1996 12:42:08 +0200 (METDST)
Subject: Re: Electron Flight Simulator

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email adress for a Demo download and further information:
dchernoff(..)aol.com
or
http://members.aol.com/smworld1000/index.htm

try it!


-----------------------------------------------------------------
Veronique Buschmann email: bushman-at-ruca.ua.ac.be
EMAT phone: +32 3 218 04 95
University of Antwerp
-----------------------------------------------------------------





From: Audette, David :      deaudette-at-corp.olin.com
Date: Thu, 6 Jun 1996 07:39:00 -0500
Subject: RE: EM/EDX: finding thin hydrocarbon coatings

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Concerning tania jones request for info on:

our lab is trying to find a way to detect very thin ( {1um thick) coatings of
stearates on the surface of a galvanized wire. our equipment consists of
only
a sem and a standard edx (no light element). any suggestions on how to "see"
the coating would be very helpful.

Tania,

I would be inclined to try imaging at low accelerating voltage on your SEM
first. A low {5 kev and not coating if possible can emphasize contaminants
on surfaces such as the stearates. Also variations in the coating is
possible to image this way but if the coating is uniform and continuous you
might want to remove some to show the contrast. After that infrared (IR)
spectroscopy is good for analysis of micron thick coatings.

Good luck,

Dave Audette
Olin Research Center
Cheshire, CT
deaudette-at-corp.olin.com




From: Keith Moulding :      mcmouldk-at-uxmail.ust.hk
Date: Thu, 06 Jun 1996 14:38:26 +0800
Subject: Electron Flight Simulator

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Hi,

I am looking a program call Electron Flight Simulator Version 2.0 for Windows.
Does anybody know where I can obtain the program from?

Thanks,

Keith.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Materials Characterisation and Preparation Centre,
Hong Kong University of Science and Technology,
Clear Water Bay,
Kowloon,
Hong Kong.

FAX: (852) 2358 2451
TEL: (852) 2358 8724
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Wed, 5 Jun 1996 22:38:15 -0800
Subject: Re: EM/EDX: finding thin hydrocarbon coatings

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Dear Tania,
I think the best way to see the stearates would be to drop the kV to 10 or
lower. I recall seeing them as dark blobs between the steel wire and Zn
coating in cross sections and I think they contained Ca. They should appear
as dark, raised, smooth blobs obscuring the wire-pulling scratches before.
Luck,
Mary

Tania wrote:} hello,
}
} our lab is trying to find a way to detect very thin ( {1um thick) coatings of
} stearates on the surface of a galvanized wire. our equipment consists of only
} a sem and a standard edx (no light element). any suggestions on how to "see"
} the coating would be very helpful.
}
} thank you in advance,
}
} tania jones
} laboratory manager
} dynamotive technologies corp.

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Keith Moulding :      mcmouldk-at-uxmail.ust.hk
Date: Thu, 06 Jun 1996 14:45:49 +0800
Subject: TEM of Receptors

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I have ask to do TEM on a sub domain of a receptor. In particular GABA-A
receptor (a protein). They are currently sitting in a Sodium Phosphate
buffer with Octyl-glucoside. As this is not my field, are there any
recommended ways to prepare the receptors for TEM, in particular how to
stain them.

Thanks in advance.

Keith Moulding.

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Materials Characterisation and Preparation Centre,
Hong Kong University of Science and Technology,
Clear Water Bay,
Kowloon,
Hong Kong.

FAX: (852) 2358 2451
TEL: (852) 2358 8724
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From: Probing & Structure :      pns-at-ultra.net.au
Date: Thu, 6 Jun 1996 14:34:50 +1000
Subject: Uranyl Acetate Safety Data

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} you wrote:
} Hi all,
}
} We are about to start using uranyl acetate for some staining applications
} but have no MSDS information regarding the handling or disposal of it. We
} can only get information for uranium products in general. The company which
} produced it no longer produces it and the company they suggested may have
} information no longer exists! A search of the WWW has also drawn a blank!
}
} If anyone out there could email this information to me it would be greatly
} appreciated as it is required with some urgency!
}
} Many thanks in advance.
}
} Colin Veitch
}
} #####################################################################
} # #
} # Colin.Veitch-at-geel.dwt.csiro.au #
} # Instrumentation Scientist #
} # CSIRO Division of Wool Technology Tel. +61 (0) 52 275611 #
} # P.O. Box 21 Fax. +61 (0) 52 275657 #
} # BELMONT Vic 3216 #
} # Australia #
} # #
} # "We see the Universe the way it is because if it were different, #
} # we would not be here to observe it." #
} # #
} #####################################################################
}
} Colin - and whoever is looking for those Material Safety Data Sheets:
}
} A great many of these are available at these two sites
} University of Utah gopher://gopher.chem.utah.edu:70/11/MSDS
} North West Fisheries http://research.nwfsc.noaa.gov/msds.html
}
} In Australia some "wise guy" decided to require that the MSDS need to be
arranged differently. They do though allow the European Community MSDS, but the
} American MSDS form is not acceptable here. Since several hundred thousand
} chemicals are imported this is a big job and hugely expensive. We have
} converted most of the EM chemical MSDS and they are available at our site.
}
} Regards Jim Darley
} Probing & Structure
} Microscopy Supplies & Accessories
}
} Phone +61 77 740 370 Fax: +61 77 892 313
} Internet Catalogue: http://www.ultra.net/~pns/
}
}
}
}
Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
Internet Catalogue: http://www.ultra.net/~pns/





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 6 Jun 1996 17:10:45 -0400
Subject: RE- ElectroPol Mg

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Message-ID: {199606070138.UAA20500-at-IndyNet.indy.net}
To: "tania-at-dynamotive.com" {tania-at-dynamotive.com} ,
Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

Subject: Time: 4:38 PM
OFFICE MEMO RE: ElectroPol Mg Date: 6/6/96

an old time report on polishing agents and etchants put out by one of the
metallurgical company labs that is no longer in existance gives the following
reagents for electrolytically polishing Magnesium:

1. 90 ml Methyl cellulose and 10 ml HCl at 10-15 V, 0.02 A/sq cm, 1-2 min;
reduce to 5 V after initial polarization

2. 93 ml carbitol and 7 ml HCl -at- 0.10 to 0.15 A/sq cm; passive film occurs
in about 1 min - remove with dilute KOH. Continue polishing an additional
20 sec.

3. 90 ml cellosolve and 10 ml HCl at 50 to 60 V for 10 to 30 sec.

4. 70 ml acetic acid, 2 ml dist. water, 28 ml 70% perchloric acid; 20 to 30
V, 0.01 A/sq cm, for 1-2 min.

5. 80 ml ethanol, 8 ml butyl cell0solve, 16 gm sodium thiocyanate. No
conditions, but stated to be Buehler reagent #10, and so it probably will
work pretty well.

6. 76 ml ethanol, 14 ml water, 5 ml 70% perchloric acid; 0.6 to 0.9 A/sq cm,
60 sec., use Ni cathode

7. A chemical polish for pure Mg: conc. nitric acid; immerse in cold acid.
Copious evol. of NO2 fumes subsides in about 1 min. Gives highly reflective
specimen with grain boundaries revealed.

Hope you find something that works satisfactorily,
W. C. Bigelow (bigelow-at-umich.edu)





From: Marc C. Brande, MS, Founder (619) 587-4830 FAX: (619) 552-1516 :      mcbrande-at-sierra.net
Date: Thu, 06 Jun 1996 19:43:43 +0000
Subject: Growing cells on slide chambers

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Message-Id: {31B734EF.43AD-at-sierra.net}
confocal-at-ubvm.cc.buffalo.edu

I would most appreciate contact information for vendors of glass or
plastic microscope slides with wells in which to culture cells for in
situ microscopy. Thanks for your efforts in advance.

Marc




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Thu, 6 Jun 1996 22:08:45, -0500
Subject: Cs and Rb

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-- [ From: Charles A. Garber., Ph.d * EMC.Ver #2.10P ] --

On June 7, Yves Thibault wrote:
========================================
I am planning to analyze with an elctron probe microanalyzer a series
of synthetic alkali-rich silicate-phosphate glasses. Some will be made
with Cs and others with Rb. I do not have proper standards for these
two elements (Cs,Rb). I was wondering if someone would know a good
source for such standards.
===============================================

You can find in the SPI "53 minerals mount" the mineral "pollucite".
It is CsSi2AlO6. The analysis shows 30.0% Cs and 0.7%Rb as well as
1.3%Na and 0.1%K. In other words - all the alkali metals but only Cs
in any reasonable amount. I doubt if it would be very much good as a
standard for a major amount of Rb. The alkali metals are too reactive
to mount as metals, which is the reason they are not in the SPI
Supplies "44 Metals" mount.

More information about these standards for microanalysis and prices can
be found on our web site given below.

Two other points: a) The "homogeneity" of this mineral is considered
outstanding as determined both by ourselves and our customers, and b)
we don't normally offer the mineral by itself, however I guess our arms
could be twisted if that really was the only mineral you wanted (needed)




From: DChernoff-at-aol.com
Date: Fri, 7 Jun 1996 01:06:57 -0400
Subject: reply

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Keith,

I was sent a reply to your initial request from Veronique Buschmann. I wanted
to correct an error in the e-mail address and the web site. The correct
e-mail address is:
dchernoff-at-aol.com

The correct web site is:

http://members.aol.com/smworld100/index.htm

Please contact me if you have any questions about the Electron Flight
Simulator program.

Best Regards
Don Chernoff
Small World






From: Ian MacLaren :      MACLARIZ-at-novell2.bham.ac.uk
Date: 7 Jun 1996 12:09:20
Subject: RE- ElectroPol Mg

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To: microscopy-at-Sparc5.Microscopy.Com

When I did some electropolishing of pure Mg I used a solution of 10%
perchloric acid dissolved in ethanol at a voltage of 38V, a temp of -30
degrees C and a fairly low flow rate in a Struers tenupol 3 twin jet
electropolisher. I can't remember where I got this recipe, maybe it was
from one of J.W. Edington's monographs on electron microscopy (unfortunately
no longer in print). It seemed to give good results but I didn't do
extensive work on Mg.

Another paper that I have (Lay, Ayed and Nouet, Acta Met. Mat., 1992, vol
40, p 2351) describes preparation of Mg specimens using twin jet chemical
polishing with 25% nitric acid in ethanol.

Hope this helps

_________________________________________________________________
Ian MacLaren, Telephone: 0121 414 3447
IRC in Materials, FAX: 0121 414 3441
The University of Birmingham, email: I.MacLaren-at-bham.ac.uk
Birmingham B15 2TT, England.
_________________________________________________________________




From: fskarl-at-goodyear.com (Frank Karl)
Date: Fri, 7 Jun 1996 08:20:35 -0500
Subject: uranyl acetate saftey (more)

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To emphasize the need for caution with uranyl acetate and echo Gib
Ahlstrand's comments on the toxicity I refer to "Nephron" 1996,
72(2)313-317 "Deliberate overdose of uranium: Toxicity and Treatment." An
abstract can be found in CAS "Forensic Chemistry" Vol 1996, issue 12 June
10 1996.

Despite these warnings uranyl acetate still remains my favorite
microchemical test for sodium!



These opinions are mine alone and have no relationship to my employer.
Thank you.

Frank Karl

They that give up essential liberty to obtain a little
temporary safety deserve neither liberty nor safety.
Benjamin Franklin








From: akracher-at-iastate.edu (Alfred Kracher)
Date: Fri, 7 Jun 1996 08:51:14 -0600
Subject: Heizer software?

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Does a company called Heizer Software (last seen at 1941 Oak Park Blvd.,
Pleasant Hill, CA) still exist? The phone number I have is no longer in
service. I bought some EXCEL templates years ago, and some do not work
anymore with newer versions. Anyone having tips, please reply personally.

Thank you!
Alfred

-----------------------------------------
Alfred Kracher
akracher-at-iastate.edu
http://www.public.iastate.edu/~akracher
vox:515 294 5439 fax:515 294 6049
-----------------------------------------






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Fri, 07 Jun 1996 08:27:08 -0500
Subject: Re: SEM-HISTO SECTIONS

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At 10:51 AM 6/6/96 EDT, you wrote:

} HI MICRO-FANS,
} I AM HAVING A BIT OF A PROBLEM SECURING WAX SECTIONS TO CARBON
} STUBS. AFTER DEPARIFFINIZATIN AND CONDUCTIVE COATING THE SECTIONS
} HAVE A TENDENCY TO LIFT OR CURL. IF ANY ONE HAS HAD ANY EXPERIENCE
} WITH PARRAFIN SECTIONS FOR SEM THE INFO WOULD BE GREATLY APPRECIATED
} THANKS,
} MATT KLEABONAS
} STRATTON VA MEDICAL CENTER
} ALBANY,NY
} TEL: (518)-462-3311 X2552
} FAX: (518)-462-1258
} E-MAIL: KLEABONAS.MATTHEW_P+-at-ALBANY.VA.GOV
}
}
*****************************
Matt -

I have attached histological sections to graphite specimen holders routinely
without problems. I cut the sections in the usual manner, floating them on a
warm water bath. I coat the graphite holder (JEOL TEMSCAN type) with the
regular histological albumin fixative (Poly Scientific Albumin Fixative
"Mayer", Cat. #S110; 70 Cleveland Ave. Bay Shore, NY 11706). I pick up the
floating paraffin section just like I was mounting it onto a glass slide,
and let it dry. You might try warming it SLIGHTLY to improve adhesion. Then
I soak it in xylene to remove the paraffin. Then I let the xylene evaporate
over night. Sometimes I put the mounted specimens into the vacuum evaporator
and pump it down over night to remove all traces of xylene before carbon
coating them. Secondary electron imaging will give you an image that readily
correlates with photographs of adjacent serial histo sections made with the
light microscope. And backscatter imaging will show up exogenous mineral
particles for EDS.

Joiner


Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Fri, 07 Jun 1996 08:27:08 -0500
Subject: Re: SEM-HISTO SECTIONS

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Message-Id: {199606071429.JAA15923-at-watson.bcm.tmc.edu}
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Mime-Version: 1.0
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At 10:51 AM 6/6/96 EDT, you wrote:

} HI MICRO-FANS,
} I AM HAVING A BIT OF A PROBLEM SECURING WAX SECTIONS TO CARBON
} STUBS. AFTER DEPARIFFINIZATIN AND CONDUCTIVE COATING THE SECTIONS
} HAVE A TENDENCY TO LIFT OR CURL. IF ANY ONE HAS HAD ANY EXPERIENCE
} WITH PARRAFIN SECTIONS FOR SEM THE INFO WOULD BE GREATLY APPRECIATED
} THANKS,
} MATT KLEABONAS
} STRATTON VA MEDICAL CENTER
} ALBANY,NY
} TEL: (518)-462-3311 X2552
} FAX: (518)-462-1258
} E-MAIL: KLEABONAS.MATTHEW_P+-at-ALBANY.VA.GOV
}
}
*****************************
Matt -

I have attached histological sections to graphite specimen holders routinely
without problems. I cut the sections in the usual manner, floating them on a
warm water bath. I coat the graphite holder (JEOL TEMSCAN type) with the
regular histological albumin fixative (Poly Scientific Albumin Fixative
"Mayer", Cat. #S110; 70 Cleveland Ave. Bay Shore, NY 11706). I pick up the
floating paraffin section just like I was mounting it onto a glass slide,
and let it dry. You might try warming it SLIGHTLY to improve adhesion. Then
I soak it in xylene to remove the paraffin. Then I let the xylene evaporate
over night. Sometimes I put the mounted specimens into the vacuum evaporator
and pump it down over night to remove all traces of xylene before carbon
coating them. Secondary electron imaging will give you an image that readily
correlates with photographs of adjacent serial histo sections made with the
light microscope. And backscatter imaging will show up exogenous mineral
particles for EDS.

Joiner


Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: DEL_ROSARIO.ARTHUR_D+-at-ALBANY.VA.GOV
Date: 07 Jun 96 08:45 EDT
Subject: neoplastic lesions arising in tattoo sites

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We are interested in compiling a series of cases related to neoplastic
lesions associated or arising at tattoo sites. We recently encountered
a case of leiomyosarcoma arising in association with tattoo site. Althoug
h the association
between tattooes and neoplasms is currently believed to be merely coincide
ntal, we still consider the possibility of a "cause and effect" relationsh
ip betwen tattooes and neoplasms arising in tattoo sites. We welcome
all cases related
to this subject.
Arthur D. del Rosario,MD
Stratton VAMC Department of Pathology
Albany,NY
E-Mail delRosario.Arthur_D+-at-Albany.VA.GOV
FAX (518) 462-1258; PHONE (518)462-3311 X2291




From: Kathy Walters :      kwalters-at-emiris.iaf.uiowa.edu
Date: Fri, 7 Jun 1996 08:52:38 -0500 (CDT)
Subject: Re: SEM-HISTO SECTIONS

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Dear Matt,

Recently I had to do a similar thing with lung tissue suspected of having
asbestos particles. We couldn't risk having the particles fall out (in a
water bath or deparaffinizing). I used a cambridge-type carbon stub,
placed the section directly onto the stub and heated it in my paraffin
oven. It adhered nicely, and as the paraffin melted the section was exposed.
I then carbon-coated it. Hope this helps.


Kathy Walters / /
Research Assistant III / /\
Center for Microscopy Research / /\ \
University of Iowa /_/ \ \
85 EMRB ____ ((O))
Iowa City, Iowa 52242 | | / /
|| / /
email: kwalters-at-emiris.iaf.uiowa.edu -----------
fax: (319)335-8049 -------------
www: http://www.uiowa.edu/~cemrf



On 6 Jun 1996 KLEABONAS.MATTHEW_P+-at-ALBANY.VA.GOV wrote:

} HI MICRO-FANS,
} I AM HAVING A BIT OF A PROBLEM SECURING WAX SECTIONS TO CARBON
} STUBS. AFTER DEPARIFFINIZATIN AND CONDUCTIVE COATING THE SECTIONS
} HAVE A TENDENCY TO LIFT OR CURL. IF ANY ONE HAS HAD ANY EXPERIENCE
} WITH PARRAFIN SECTIONS FOR SEM THE INFO WOULD BE GREATLY APPRECIATED
} THANKS,
} MATT KLEABONAS
} STRATTON VA MEDICAL CENTER
} ALBANY,NY
} TEL: (518)-462-3311 X2552
} FAX: (518)-462-1258
} E-MAIL: KLEABONAS.MATTHEW_P+-at-ALBANY.VA.GOV






From: jan_ringnalda-at-pei.philips.com (Jan Ringnalda)
Date: Fri, 7 Jun 1996 12:50:50 -0400
Subject: Job availability

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Mime-Version: 1.0

TECHNICAL SALES

Can you operate state-of-the art microscopy equipment? Like to try
sell it?

If so, then due to our continued growth, we'd like to talk with you.
Philips is a leading manufacturer of electron microscopes used in
laboratories around the world. We are looking for highly motivated
professionals who have demonstrated success in developing long-term
consultative relationships with senior scientists. Excellent verbal
and interpersonal skills and at least a B.S. in science or engineering
are required.

Sales Managers responsible for selling Scanning and Transmission
Electron Microscopes in the Midwest and Southwestern U.S. Based in
the Midwest, and Phoenix, AZ.

Product Manager responsible for marketing and sale of Philips' Defect
Review Tool for the semiconductor industry. Based in Phoenix, AZ.

These positions offer an excellent compensation package with growth
opportunities. Please send confidential resume, including salary
history and position desired to: Lisa Stitt, Employment &
Compensation Specialist. (Local interviews will be arranged.)

PHILIPS ELECTRONIC INSTRUMENTS COMPANY
85 McKee Drive, Mahwah, New Jersey, 07430
FAX: 201-529-0896
Internet Address: lisa_stitt-at-pei.philips.com

OR: Just hit reply and I'll forward stuff to Lisa.
Cheers, Jan




From: RMCCryo-at-aol.com
Date: Fri, 7 Jun 1996 15:26:50 -0400
Subject: Paraffin Bath Needed for Auto-Technicon 2A

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A colleague of mine needs a paraffin bath for her very old Auto-Technicon
paraffin tissue processor. It's beyond repair. Can anyone help?

Many Thanks!

Bob Chiovetti
Applications Lab Manager
RMC
(520) 889-7900
Fax (520) 741-2200
RMCBTLI-at-aol.com




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: Fri, 7 Jun 1996 12:33:05, -0500
Subject: Polypropylene characterization

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-- [ From: Charles A. Garber., Ph.d * EMC.Ver #2.10P ] --

Liam MacManus wrote:
==============================================
I'm looking into the surface modification of polypropylene (PP), using
UV/ozone techniques. What I would like to know is if anyone can give
me suggestions on distinguishing between a biaxially oriented (and
therefore crystalline) PP and an amorphous or semi-crystalline PP.
I'm trying DSC analysis, and am looking into IR studies. Any other
suggestions?
================================================
The best way to do this is by x-ray diffraction and another way,
depending on the thickness of your samples would be by LM looking at
the birefringence (but this might require some messy sample prep and
sectioning). In your XRD patterns, the biaxially oriented material
will have "spots" at both poles and also equatorially. Uniaxially
oriented material will have only two spots. And of course, unoriented
(but crystalline) material will just have a uniform "ring". Purely
"amorphous" polypropylene would in essence have just an amorphous
"halo".

No fancy single crystal orienter is required, just a plain vanilla
garden variety XRD system. For pedagogical purposes, a "flat plate
photo" (really old fashioned) might even be better.

I think that DSC would give you ambiguous results that would not have a
unique interpretation. Dichroic ratio measurements could be done by IR
but XRD is really the way to do it.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.com

Take a look!
####################################
WWW: http://www.2spi.com
####################################
======================================================





From: Scott Hollington :      sco.umc2-at-Mail.health.ufl.edu
Date: Fri, 07 Jun 1996 12:32:49 -0400
Subject: unsubscribe

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From: Woody.N.White-at-mcdermott.com
Date: 7 Jun 96 15:46:00 -0500
Subject: Kevex EDS (AIA) S-ware ?

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I have a Kevex 8005 sys & have question re: AIA s-ware
package. Doing feature analysis, save feature data to
file type .FTR (is not ASCII) for each image/frame.
Can make data histogram from each image/frame/file.
NEED to make one histogram containing data from
multiple images. Therefore need to concatenate/merge
data from multiple .FTR files into one. HOW????
Even if I "tell" the pgm to "save/external" and have
the file types set to ASCII, it still seems to save
as file unreadable by conventional software. I have
DOS/PC setup to read the 44mb DEC format bernoullis
into dos format so I can "fiddle" w/data on PC, but
cannot get that far. Kevex has been no help.

Thanks in advance,

Woody Work: woody.n.white-at-mcdermott.com
Home: woody.white-at-worldnet.att.net

Babcock & Wilcox Research
Lynchburg, VA




From: Haroon Ikram :      haroon-at-zirc.chem-eng.toronto.edu
Date: Fri, 7 Jun 1996 20:09:51 -0500 (CDT)
Subject: Paper for printing digital images...

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Dear Microscopist,

I've got some TEM/STEM digitized images from a JEOL 2010 FETEM and would
like to print them using a laser printer. What quality of paper would you
suggest to get a good, clear printout.

I'll appreciate any help.
Thanks.
Haroon Ikram,
Deptt. of Met. & Mat. Science
U of T, Canada.




From: Gary Login :      glogin-at-bih.harvard.edu
Date: Fri, 7 Jun 1996 22:21:18 -0400
Subject: Re: LM/TEM/plants/seeds

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In message {9605311255.AA12167-at-phage.cshl.org} Tamara Howard in Cold Spring
Harbor Laboratory writes:
} Helpful people:
} I'm having trouble with some seed material...we need to look at the
} pericarp(s) of these guys through the seeds' development. The early stages
} were cake, but I'm having trouble with older material - it is more "woody"
} as we go. I've found several seed prep protocols, but I was hoping someone
} out there has a tried and true method...


Howard, I recommend the following references on microwave-accelerated
preparation of plant and seed tissue for morphologic studies.

1993. Microwave miniprep of total genomic DNA from fungi, plants, protists and
animals for PCR. BioTechniques. 15:438-441.

Benhamou, N., S. Noel, J. Grenier, A. Asselin. 1991. Microwave energy fixation
of plant tissue: an alternative approach that provides excellent preservation of
ultrastructure and antigenicity. J Electron Microsc Tech. 17:81-94.

Giberson, R.T., R.S. Demaree, Jr. 1995. Microwave fixation: understanding the
variables to achieve rapid reproducible results. Microsc Res Tech. 32:246-254.

Heumann, H.G. 1992. Microwave-stimulated glutaraldehyde and osmium tetroxide
fixation of plant tissue: ultrastructural preservation in seconds. Histochem.
97:341-347.

Hoefert, L.L., J.D. McCreight, R.D. Christie. 1992. Microwave enhanced staining
for plant virus inclusions. Biotechnic & Histochem. 67:40-44.

Kang, Z., R. Rohringer, J. Chong, S. Haber. 1991. Microwave fixation of
rust-infected wheat leaves. Preservation of fine structure and detection of
cell surface antigens, lectin- and sugar-binding sites. Protoplasma. 162:27-37.

Kartnig, T., D. von Horsten, C. Lassnig, B. Classen. 1995. [The application of
microwave energy in preparation of herbal drugs. 2.]. Pharmazie. 50:498-504.

Login, G.R., A.M. Dvorak. 1994. Methods of microwave fixation for microscopy. A
review of research and clinical applications: 1970-1992. Prog Histochem
Cytochem. 27/4:1-127.

Login, G.R., A.M. Dvorak 1994. The Microwave Toolbook. A Practical Guide for
Microscopists. Beth Israel Hospital, Boston.

Medina, F.J., A. Cerdido, R. Marco. 1995. Microwave irradiation improvements in
the silver staining of the nucleolar organizer (Ag-NOR) technique. Histochem
Cell Biol. 103:403-13.

Medina, F.J., A. Cerdido, M. Maroto, M. Manzanares, R. Marco. 1994. Enhancement
of the immunocytochemical detection of antigens by microwave irradiation.
Benefits and limitations analysed in isolated plant nuclei and Drosophila
embryos in toto. Histochemistry. 102:45-50.

Please contact me if you have additional questions.


Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676


Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676





From: bjg-at-uniwa.uwa.edu.au (Brendon J. Griffin)
Date: Sat, 8 Jun 1996 16:35:01 +0800
Subject: Re: Paper for printing digital images...

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} Dear Microscopist,
}
} I've got some TEM/STEM digitized images from a JEOL 2010 FETEM and would
} like to print them using a laser printer. What quality of paper would you
} suggest to get a good, clear printout.
}
} I'll appreciate any help.
} Thanks.
} Haroon Ikram,
} Deptt. of Met. & Mat. Science
} U of T, Canada.

Haroon

We are printing images from our SEM's on a 1200dpi Lexmark Optra R sports
plus. After some experimentation we have settled on the Canon Colour
Copier paper. From memory it is around 80gsm and is excellent. The cost
per page is around 3c (Oz).

We had not thought much about paper in our printers before this but were
amazed at the variations between "white" paper and so I strongly suggest to
all sharing our state of ignorance to have a close look at the paper they
use.

Obviously we have no financial interests in anything!

Cheers
Brendon J. Griffin
Centre for Microscopy and Microanalysis
The University of Western Australia
Nedlands, WA, AUSTRALIA 6907
ph 61-9-380-2739 fax 61-9-380-1087





From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Sat, 8 Jun 1996 08:35:13 -0500
Subject: Ultramicroscopy, what has happened

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The subject says it all - does anyone know what has happened to
Ultramicroscopy, which does not appear to have published an issue since
November 1995. I am getting a little annoyed at refering to one of my
papers as "Ultramicroscopy, in press (1995)" - is the journal dead?




From: WINSTON_VERN :      winsvern-at-cwis.isu.edu
Date: Sat, 8 Jun 1996 11:16:13 -0600 (MDT)
Subject: Ultramicroscopy, what has happened

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unsubscribe

Vern Winston






From: Steven Schwarz :      sschwarz-at-morgan.ucs.mun.ca
Date: Sat, 8 Jun 1996 20:19:58 -0230 (NDT)
Subject: unsubscribe

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unsubscribe

==============================================================================
Steve Schwarz sschwarz-at-morgan.ucs.mun.ca
Dept. of Earth Sciences
Memorial University of Newfoundland
Newfoundland
CANADA
A1B 3X5
1-709-737-8142
-737-2589 FAX
******************************************************************************




From: Childs, Gwen :      childs-at-mbisrv.utmb.edu
Date: Sat, 8 Jun 1996 23:09:00 -0500
Subject: unsubscribe

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From: Emeylan-at-aol.com
Date: Sun, 9 Jun 1996 09:41:39 -0400
Subject: Re: used ultra wide 10x eyepieces

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I have a pair of CFW N 10x 84220
They are brand new, still in original box.

If you are interested, please reply to

76227.776-at-compuserve.com

Emile Meylan
SERCO Technical Services, Inc.




From: ERJ-at-vetmed1.vetmed.ufl.edu
Date: Mon, 10 Jun 1996 10:37:12 EST
Subject: fluorescence microscopy quanitation

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To: microscopy-at-Sparc5.Microscopy.Com

Microscopists: We have recently begun doing fluorescence microscopy of
fluorescent-antibody labelled virus grown in cell monolayers. Are there any
accepted techniques for quanitation of the fluorescent signal with a CCD
camera or other video system. Is there software available to accomplish this
on a Mac platform. We have a conventional fluorescent microscope with a Hg
lamp.

Thanks for any suggestions,

Elliott Jacobson
Elliott Jacobson
Professor
Department of Small Animal Clinical Sciences
P.O. Box 100126
College of Veterinary Medicine
University of Florida
Gainesville, Florida 32610, USA
Phone: 904-392-4700 X4773
Fax: 904-392-6125
E-Mail: ERJ-at-vetmed1.vetmed.ufl.edu
WEB Site: www.vetmed.ufl.edu






From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Mon, 10 Jun 1996 11:00:51 -0600
Subject: Optical microscopes for metallurgy

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We are trying to choose between 3 compound microscopes for bright field,
dark field, and DIC: Zeiss Axiotech with transmitted light box, Nikon
Labophot-2/2A with Epi illuminator, and Olympus BX60/M.

I'd like to ask a few questions about these microscopes. If you'd be
willing to help us, please reply to my Email address. Thanks!

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(708)252-7194
FAX: (708)252-4798






From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 6/6/96 6:03 PM
Subject: video equipment for microscopy

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We are in the midst of purchasing some digital equipment for our microscopy lab.
If you are a regular to the forum you may remember being asked for some
suggestions. Well now we are getting to the task of sorting through all the
files we have gathered. I'm looking for opinions of those of you working with
equipment in the field who have no vested interest in any specific companies.
Please give positive and negative drawbacks and try to address the following
questions:

1. Does anyone have equipment hooked up to a cambridge S90 and if so what do
you have?
2. Are you currently using semicaps (Genie or 1000)?and give opinion of system
3. Other than semicaps is there any other company that uses active beam control
(ie controls electron beam)to maximize resolution?
4. Is your system user friendly and geered for multiple users?
5. Is your system Mac or not?

Just some background information:
We have an electron microscopy class of 24 students and we are trying to expose
them to the art of digital archiving as well as aleviate some time spent in the
darkroom. We also in our biology department have 10 computer stations which are
on our network.(Macs). The system is going to be used for capturing images from
the sem,lm and copystand , and tem. These images will be labeled etc. and down
loaded through the network for tutorials on the computers. Images will be
supported in colour and can also be used for manuals and manuscripts.

Thanks,

sincerely Debbie Lietz
Electron Microscopy Suite
Department of Biology
Trent University
Peterborough, Ontario
K9J 7B8
email DLietz-at-trentu.ca






From: ERJ-at-vetmed1.vetmed.ufl.edu
Date: Mon, 10 Jun 1996 14:20:53 EST
Subject: frame grabbers

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To: microscopy-at-Sparc5.Microscopy.Com

Does anyone have any information regarding frame grabbers for Macs? I'm
looking for something like Snappy which is made for PC's.
thanks
Elliott Jacobson
Professor
Department of Small Animal Clinical Sciences
P.O. Box 100126
College of Veterinary Medicine
University of Florida
Gainesville, Florida 32610, USA
Phone: 904-392-4700 X4773
Fax: 904-392-6125
E-Mail: ERJ-at-vetmed1.vetmed.ufl.edu
WEB Site: www.vetmed.ufl.edu






From: lag-at-pegasus.cc.ucf.edu (Lucille A. Giannuzzi)
Date: Mon, 10 Jun 1996 15:55:56 -0400
Subject: fuzzy TEM plate numbers

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I've been getting fuzzy plate numbers using a Philips EM430. The images
are clear, but the identification numbers are unreadable (this has been
observed consistently with only one camera). Has anyone ever experienced
this strange behavior before? Does anyone know how to fix the problem
without taking the camera out of rotation (two other cameras yield sharp,
dark, and clear identificaton numbers)?

Thanks in advance!

**************************************************************************
Lucille A. Giannuzzi, Ph.D. phone: 407 823-5770
University of Central Florida fax: 407 823-0208
Materials Science Program email: lag-at-pegasus.cc.ucf.edu
Dept. of Mechanical and Aerospace Eng.
Orlando, FL 32816-2450
**************************************************************************






From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Mon, 10 Jun 1996 16:07:50 -0500
Subject: Kevex monitor wanted

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Message-Id: {199606102109.QAA09360-at-mailhub.iastate.edu}
X-Sender: wes-at-pop.ameslab.gov
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Mime-Version: 1.0
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We have a Kevex Delta V EDS system that has apparently lost its RGB monitor.
That is, its brightness has been fading away for some time and now it seems
to have sync problems. We have swapped it for another Kevex monitor on
campus and the system then works fine (thanks, Bruce). However, Bruce is
going to need his monitor back soon and we would like to find a "permanent"
replacement.

We are probably going to replace our system within the year, so we are not
interested in spending $1K+ to replace the monitor. But if someone out there
has a functioning Kevex monitor they would like to sell for a few dollars or
that they wouldn't mind loaning us, we would be very happy to talk with them.

Or, if there is some way to adapt a more standard color monitor to the job,
I am open for ideas. I have been told that the vertical scan rate of the
Kevex monitor is 57.9 Hz and the horizontal rate is 17.2 kHz, which is way
below normal PC monitors (upwards of 30 kHz).

Once again, any help will be appreciated.
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Chris Frethem (CBN) :      frethem-at-lenti.med.umn.edu
Date: Mon, 10 Jun 1996 17:52:25 -0500 (CDT)
Subject: unsubscribe

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unsubscribe frethem-at-lenti.med.umn.edu



=======================================================================
Chris Frethem (612)624-4652 (voice)
Cell Biology & Neuroanatomy (612)624-8118 (FAX)
U of MN, Minneapolis e-mail: frethem-at-lenti.med.umn.edu








From: bjg-at-uniwa.uwa.edu.au (Brendon J. Griffin)
Date: Tue, 11 Jun 1996 08:08:18 +0800
Subject: Reconditioned Ion Pumps for Philips TEM's

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} Mime-Version: 1.0
} Content-Type: text/plain; charset="us-ascii"
} Date: Mon, 10 Jun 1996 15:27:36 +0800
} To: bjg-at-uniwa.uwa.edu.au
} From: andy-at-earwax.pd.uwa.edu.au (Andrew Johnson)
} Subject: Reconditioned Ion Pumps for Philips TEM's
}
} Reconditioned Ion Pumps (SIP's) for Philips EM 400 series TEM's
}
} Can anyone out there help with a supplier who can exchange or recondition
} the ion pump of a Philips TEM. The pumps are made by Edwards, apparently a
} special for Philips. Model/Code no. EP100/B036-06-000. The pump body would
} require to be sawn open and rewelded to renew the electrodes.
}
}
}
}
Brendon J. Griffin
Centre for Microscopy and Microanalysis
The University of Western Australia
Nedlands, WA, AUSTRALIA 6907
ph 61-9-380-2739 fax 61-9-380-1087





From: Tang Ee Koon :      medlab2-at-leonis.nus.sg
Date: Tue, 11 Jun 1996 08:41:15 +0800 (SST)
Subject: Scannertron Enlarger

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Hello!All Microscopists,
Does anyone of you out there has any information on Scannertron
Enlarger? My Unit is thinking of getting one, so if you have any
information of the supplier(s) in Singapore, please let me know.
Thank you very much.



Regards,
Catherine Tang
Electron Microscopy Unit
National University of Singapore




From: Jan D'Haen :      jdhaen-at-luc.ac.be
Date: Tue, 11 Jun 1996 10:19:16 +0200 (MET DST)
Subject: depassivation of microelectronic components

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We are looking for a method to depassivate microelectronic components.
The components have to be depassivated to allow a SEM research.
For the depassivation we are thinking on ion beam methods and on
chemical methods.
In particular we are looking for the depassivation of Al stripes with a
nitride passivation layer upon it. Has someone a recipe for the chemical
depassivation of microelectronic components and in particular of
passivated Al stripes.

Thanks in advance!


Jan D'Haen
Limburgs Universitair Centrum
Institute for Materials Research
Materials Physics Division
Universitaire Campus
Wetenschapspark 1
B-3590 Diepenbeek
Belgium

tel. : 32-11-268883
fax. : 32-11-268899
e-mail: jdhaen-at-luc.ac.be




From: Jan D'Haen :      jdhaen-at-luc.ac.be
Date: Tue, 11 Jun 1996 11:21:51 +0200 (MET DST)
Subject: depassivation of microelectronic components

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We are looking for a method to depassivate microelectronic components.
The components have to be depassivated to allow a SEM research.
For the depassivation we are thinking on ion beam methods and on
chemical methods.
In particular we are looking for the depassivation of Al stripes with a
nitride passivation layer upon it. Has someone a recipe for the chemical
depassivation of microelectronic components and in particular of
passivated Al stripes.

Thanks in advance!


Jan D'Haen
Limburgs Universitair Centrum
Institute for Materials Research
Materials Physics Division
Universitaire Campus
Wetenschapspark 1
B-3590 Diepenbeek
Belgium

tel. : 32-11-268883
fax. : 32-11-268899
e-mail: jdhaen-at-luc.ac.be





From: lisa_stitt-at-pei.philips.com (Lisa Stitt)
Date: Tue, 11 Jun 1996 09:05:50 -0400
Subject: Job Opportunity

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TECHNICAL SALES--Midwest Territory

EDAX International, a manufacturer of detectors and analyzers for
microanalysis, seeks an aggressive salesperson with a BA or BS degree
and technical background. Self-starter with 3-5 years sales
experience in analytical or capital equipment perferred. We offer a
competitive salary, commissions and an excellent benefit package.
Please forward confidential resume and salary history to:

Lisa Stitt, Human Resources
EDAX International
85 McKee Drive, Mahwah, New Jersey 07430
FAX: 201/529-0896
Internet Address: lisa_stitt-at-pei.philips.com




From: lisa_stitt-at-pei.philips.com (Lisa Stitt)
Date: Tue, 11 Jun 1996 09:05:50 -0400
Subject: Job Opportunity

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TECHNICAL SALES--Midwest Territory

EDAX International, a manufacturer of detectors and analyzers for
microanalysis, seeks an aggressive salesperson with a BA or BS degree
and technical background. Self-starter with 3-5 years sales
experience in analytical or capital equipment perferred. We offer a
competitive salary, commissions and an excellent benefit package.
Please forward confidential resume and salary history to:

Lisa Stitt, Human Resources
EDAX International
85 McKee Drive, Mahwah, New Jersey 07430
FAX: 201/529-0896
Internet Address: lisa_stitt-at-pei.philips.com




From: bjg-at-uniwa.uwa.edu.au (Brendon J. Griffin)
Date: Tue, 11 Jun 1996 08:08:18 +0800
Subject: Reconditioned Ion Pumps for Philips TEM's

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Message-ID: {n1377635090.11629-at-mse.engin.umich.edu}
"microscopy-at-Sparc5.Microscopy.Co" {microscopy-at-Sparc5.Microscopy.Com}
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Reply to: RE} Reconditioned Ion Pumps for Philips TEM's

The Duniway Stockroom Corp., (1305 Space Park Way, Mountain View, California
94043; FX: 415-965-0764) reconditions ion pumps and other vacuum apparatus.
I would expect that they might be able to work on the pump for your Philips
EM.
Best regards, and good luck with your problem,
Wil Bigelow (bigelow-at-umich.edu)

--------------------------------------


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From: Lou Ann Miller :      lmiller-at-ux1.cso.uiuc.edu
Date: Tue, 11 Jun 1996 10:14:08 -0500
Subject: Central States Microscopy Society --- New Web Page

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Message-Id: {31BD8D40.7ADC-at-ux1.cso.uiuc.edu}

The Central States Microscopy Society has a new Web Page at URL:


http://www.cvm.uiuc.edu/Homepages/LouAnnMiller/CSMS/csms.html


Much of the page is under construction. But information on the
upcomming meeting and updates, times etc for June 28th are kept on
URL:

http://www.cvm.uiuc.edu/Homepages/LouAnnMiller/CSMS/spring96

The goal was to start up a homepage, and then to get user input on
what to add, what to take off etc.


*******************So we need user input**************************

Send to Lou Ann Miller,
(email anchor also at bottom of CSMS Home page):
==================================================

suggestions

pictures you are proud of and want to show on the web

technique tips

other local microscopy meetings

etc etc
=================

Thanks!!!

LA
***********************
Lou Ann Miller
Incomming Secretary for CSMS

Microscopic Imaging Laboratory
College of Veterinary Medicine
University of Illinois
2001 S Lincoln Ave
Urbana, Illinois 61801
217-244-1566
lamiller-at-ux1.cso.uiuc.edu

Microscopic Imaging Lab Home Pages:
http://www.cvm.uiuc.edu/MicImagLab/MicImagLab.html

Personal Home Pages:
http://www.cvm.uiuc.edu/Homepages/LouAnnMiller/LAM.html
***********************




From: Lou Ann Miller :      lmiller-at-ux1.cso.uiuc.edu
Date: Tue, 11 Jun 1996 10:40:22 -0500
Subject: Central States Microscopy Soc ---Microwaving Discussion

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Message-Id: {31BD9366.7AE4-at-ux1.cso.uiuc.edu}

Greetings!

I¹m to lead a very very informal discussion on Microwave Techniques at
the Spring CSMS meeting on June 28th.

For meeting details check:
http://www.cvm.uiuc.edu/Homepages/LouAnnMiller/CSMS/Spring96

I will bring some pictures and small props to pass around, and I plan
to bring ugly pictures and mistakes as well as pictures I don¹t
grimace at.

********WHAT I NEED IS FOR ANYONE WHO HAS DONE MICROWAVING AT ALL TO
BRING THEIR PICTURES ALSO.*****
This is will defiantly NOT be a critic. The goal is :

* Those of us ( like me) who have only tried 1 or 2 protocols can
see what other protocols look like and to acquire a feel for what is
possible, and new things we might like to try.

* Bring the failures, others may recognize past failures that look
like yours and have an ideal at what step something could be changed.

* Share Technique tips that you have invented or found useful.

* Discover where Microwaving can be useful with procedures not
normally thought to use microwave with.

* Bring ideals on how you started up your Microwave, calibration etc.

=====**** Bring a copies of your protocols to share , I¹ll bring some
of mine.


Thanks much!

Lou Ann
--
***********************
Lou Ann Miller
Microscopic Imaging Laboratory
College of Veterinary Medicine
University of Illinois
2001 S Lincoln Ave
Urbana, Illinois 61801
217-244-1566
lamiller-at-ux1.cso.uiuc.edu

Microscopic Imaging Lab Home Pages:
http://www.cvm.uiuc.edu/MicImagLab/MicImagLab.html

Personal Home Pages:
http://www.cvm.uiuc.edu/Homepages/LouAnnMiller/LAM.html
***********************




From: RSARTORE-at-FTMON.ARL.MIL (Sartore, Richard G.)
Date: Tue, 11 Jun 1996 08:07 -0500 (EST)
Subject: Re: depassivation of microelectronic components

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Good Morning Jan:
We had used wet etching several years
ago but found it to be
marginal. Invariably the area of interest didn't etch at same rate as
rest of chip or
was never completely removed. Buffered HF was typically used for SiO2 and
also
for nitride but at a slower rate ( which can be an advantage ).
Literature specifies
hot ( I think, 90C, must check for accuracy) phosphoric acid for Si3N4.
This works but
is more complicated in handling and safety of use, especially heating the
acid
We recently purchased a plasma etcher for this
purpose, to eliminate the
safety problems in handling the wet chemicals and the disposal of
hazardous chemicals.
The unit has worked very well for the removal of Si oxides and nitrides.
Besides the
benefit of not having to deal with the wet chemicals, the plasma etched
samples are
much cleaner for SEM inspection, with essentially no residue. Plus
todate, I have encountered only minor problems with non-uniform etching
compared to the wet
etch technique.

----------------------------------------------------------
Richard Sartore
US Army Research Laboratory
AMSRL-PS-DC
Fort Monmouth, NJ 07703-5601
908-427-2261
FAX 908-532-0156
rsartore-at-arl.mil
----------------------------------------------------------






From: bsgphy3-at-uconnvm.uconn.edu (JIM ROMANOW)
Date: Tue, 11 Jun 1996 12:52:15 -0500
Subject: O-rings

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Thank you all for the o-ring source information. Your help is much appreciated.

Regards,

Jim Romanow
Electron Microscopy Facility
Physiology and Neurobiogy Department
The University Of Connecticut
Storrs
bsgphy3-at-uconnvm.uconn.edu
(860) 486-2914 voice
-1936 fax






From: chender-at-umich.edu (Carl Henderson)
Date: Tue, 11 Jun 1996 09:00:39 -0400
Subject: Re: Kevex monitor wanted

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} We have a Kevex Delta V EDS system that has apparently lost its RGB monitor.

} Or, if there is some way to adapt a more standard color monitor to the job,
} I am open for ideas. I have been told that the vertical scan rate of the
} Kevex monitor is 57.9 Hz and the horizontal rate is 17.2 kHz, which is way
} below normal PC monitors (upwards of 30 kHz).

I have replaced two monitors on older Kevex 8000 units with Electrohome
monitors. These monitors also work on a Delta system in our lab. In fact,
the monitor supplied by Kevex for the Delta is an Electrohome monitor.

Some cabling changes were needed to adapt the monitor to the 8000 systems;
no change was needed for the Delta system. On the 8000 units, an auxillary
speaker is be needed, since the new monitor does not have one.

The horizontal scan rate on the Kevex systems is down around 15-16 kHz,
which makes it difficult to adapt commonly used computer multisync monitors
to this task. This said, be aware that the Electrohome monitor is not
cheap, though it is less expensive than a Kevex replacement part.

The model number is ECM1411, which is a 14" color monitor with 0.28mm dot
pitch and digital memory sizing. Input is via 5 BNC analog or 9 pin
TTL/VGA. In fact, the versatility of this monitor makes me believe I will
use it on some other computer system when the Kevex units are retired.

There is also a model available with anti-magnetic shielding, if your
monitor is likely to interfere with the electron column. I do not have
this version and I have not seen any problems. The SEM column is six feet
away (Hitachi S-570) and the EMPA column is seven feet away (Cameca MBX).

Electrohome (Kitchener, Ontario) can be reached at 519-744-7111. The local
distributor for our area was Blue Ash Electronics (Cincinnati, Ohio) at
800-762-5584.

DISCLAMER: I have no financial interests in Electrohome or Blue Ash Electronics.

Carl


======================================
Carl Henderson
University of Michigan
Electron Microbeam Analysis Laboratory
2501 C.C. Little Bldg.
Ann Arbor, MI 48109-1063






From: Doug Keene :      DRK-at-shcc.org
Date: Tue, 11 Jun 1996 09:52:10 -0500 (cdt)
Subject: Nickel based staining techniques

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We are interested in Nickel based staining techniques for
the EM localization of polypeptides containing six
histidines. Does anyone have any experience in this area or
suggestions as to where to look for a protocol?

Many thanks,

Doug Keene
Shriners Hospital for Crippled Children
Connective Tissue Research Group
Electron Microscopy Facility
----------------------
Doug Keene
DRK-at-shcc.org






From: Woody.N.White-at-mcdermott.com
Date: 11 Jun 96 08:23:00 -0500
Subject: Kevex AIA answer!

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I now have an answer to my AIA file handling question! ...Recieved
a call from what seems to be the only person who knew at Kevex.
My thanks to him. What I needed was (buried) in the manual
[redface], but if you have ever experienced manuals for low sales
volume/complex software, you understand my not finding it!
-- & I'm not the only one...

FYI... The commands to sum data are:

Enter:

REC/FEA {cr} -You will be promped for filename, don't enter!

Hit- Blue ^ arrow key ("runfile") -A list of feature data files
will be returned to CRT.

Using up/down (black) arrow keys, select (highlight) file to add.

Select each file (to add) by hitting "acquire" key while
filename is highlighted. Beware: This also outputs FN to
Lprinter. If it is off-line/locked-up it may cause an apparent
computer halt????

When finished selecting files hit {cr} . Sumation of files will
load to memory.
-------------------
Associated info: Saving data in ASCII format... Is in manual,
but so obscure one may never find it... Also, while assured that
the following procedure works, it dosen't on my system. It is
believed that is because of my drive configuration (Dual 44 Mb
Bernoulli - Data (default) saved to "DL7:").???

Be sure SETUP/SYS, item 4 is set to ASCII or Lotus (your choice)
and appropriate data is in memory.

Instead of SAV/EXT.... Use the command FILE/MORPH, FILE/XRAY,
FILE/ALL....

Enter the FN, for which you will be prompted, {cr}

This should save the data in a FType .TXF or .PRF dependent
on choice set in item 4 above.

This will not work on my system as configured... When I have
more time maybe I will "play" with it more... Good Luck ALL!

Woody woody.n.white-at-mcdermott.com (work)
woody.white-at-worldnet.att.net (home)




From: Sally Shrom :      sally-at-retina.anatomy.upenn.edu
Date: Tue, 11 Jun 1996 15:55:54 -0400 (EDT)
Subject: TEM biology

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Posted-Date: Tue, 11 Jun 1996 16:01:14 -0400

Most labs dehydrate tissue in ethanol when processing for E.M.

I have always used methanol. Is there logic in using one alcohol or the
other?

Sally Shrom





From: Charles A. Garber :      103532.3325-at-CompuServe.COM
Date: 11 Jun 96 09:35:11 EDT
Subject: Passivation layer removal

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Jan D'haen wrote:
============================================
} We are looking for a method to depassivate microelectronic components. The
components have to be depassivated to allow a SEM research. For the
depassivation we are thinking on ion beam methods and on chemical methods. In
particular we are looking for the depassivation of Al stripes with a nitride
passivation layer upon it. Has someone a recipe for the chemical depassivation
of microelectronic components and in particular of
passivated Al stripes.
=============================================
The easiest, fastest, and (literally) "cleanest" way to do this is with reactive
plasma etching. I say "cleanest" because unlike chemical methods, you do not
redistribute around anything such as corrosion product. Hence, everything is
left, in situ, just where it is, making for good analysis possibilities, be it
by EDS or Auger, etc.

You have a choice of two different approaches, one being "isotropic" etching,
the other being "anisotropic" etching. The former is cheaper and faster, but
one does get some amount of undercutting. The latter gives no undercutting, but
the etching is much slower (etching times typically 90 min vs. 30 min.), but is
needed only if your "lines" are getting down well under one um. The actual
figure depends on the thickness of the nitride layer you are removing.
Undercutting may or may not be important for you.

Several firms do make small inexpensive table-top type equipment to do this,
including SPI Supplies. You can find out more about the family of the SPI
"Plasma Preps" on our web site, given below. Click on catalog and then plasma
etchers. You will see nice examples of both isotropic and anisotropic etching
and a comparison of the same device etched using the two different approaches,
e.g. with vs. without undercutting.

To remove nitride layers, although there have been publications suggesting
improved (e.g. faster, less "residue") results with some of the more exotic and
more expensive reactive fluoro-type gasses, it seems like most people get just
as acceptable results using plain ordinary (and inexpensive, relatively
speaking) CF4 gas.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.com

###########################
WWW: http://www.2spi.com
###########################
======================================================





From: garyc-at-stud.unit.no (Gary)
Date: Tue, 11 Jun 1996 19:38:18 +0200 (MET DST)
Subject: Subscribe

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Subscribe garyc-at-storm.unit.no

/////////////////////////////////////////////////////////////////
// // //

// Gary Chinga // email :garyc-at-james.avh.unit.no //
// Plantebiosenteret // WWW :http://www.nvg.unit.no/~gary //
// NTNU, 7055 Dragvoll // phone : 73590168 //
// Norway // fax : 73590177 //
// // //
/////////////////////////////////////////////////////////////////






From: marilyn-at-cemmsa.adelaide.edu.au (Marilyn Henderson)
Date: Wed, 12 Jun 1996 10:15:01 +0900
Subject: Immunolabelling Workshop Announcement

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Message-Id: {199606120007.AAA29938-at-traminer.cemmsa.adelaide.edu.au}
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-------------------------------------------------------------------

FLINDERS INSTITUTE FOR HEALTH TECHNOLOGY TRAINING (FIHTT) in association
with THE CENTRE FOR ELECTRON MICROSCOPY AND MICROSTRUCTURE ANALYSIS
(CEMMSA)

IMMUNOGOLD LABELLING FOR ELECTRON MICROSCOPY

INTENSIVE INTRODUCTORY COURSE - MARCH 10-12, 1997

First Announcement

This succesful course was run first in 1995 and was rated as 'excellent'
'by participants. In 1997 the course will be run under the auspices of
FIHTT at The Centre for Electron Microscopy and Microstructure Analysis
(CEMMSA ) at the University of Adelaide, South Australia. Topics covered
will be the theoretical and practical aspects of routine and cryo
immunogold labelling for EM - including 'hands-on' experience. Techniques
are suitable for research or diagnostic applications. CEMMSA is centrally
located with easy access to transport, Adelaide city centre and a wide
range of accomodation.

ORGANIZERS:

JOHN STIRLING, Flinders Microsope Imaging and Analysis Facility (FMIAF),
Flinders Medical Centre and
MARILYN HENDERSON (CEMMSA), The University of Adelaide.

COST: Full-time students AU$175; others AU$350.

Registration includes consumables, lunch and morning and afternoon tea.

Registrations close Friday 31 January, 1997.

For an application form, further information or to register your interest
write to:
JOHN STIRLING
IMMUNOGOLD LABELLING COURSE
FLINDERS INSTITUTE OF HEALTH TECHNOLOGY TRAINING
FLINDERS MEDICAL CENTRE
BEDFORD PARK SA 5042
SOUTH AUSTRALIA

OR CONTACT
JOHN STIRLING at Email address: John.Stirling-at-flinders.edu.au
Tel: 08 -204 4669 (International 618 204 4669)
Fax: 08 374 1437 (International 618 374 1437)

OR Marilyn Henderson at Email address: marilyn-at-cemmsa.adelaide.edu.au







From: caron-at-lisa.polymtl.ca (Mario Caron)
Date: Tue, 11 Jun 1996 20:53:45 -0400
Subject: Re: depassivation of microelectronic components

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Message-Id: {9606120052.AA00716-at-lisa.polymtl.ca}
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We are also use RIE (Reactive Ion Etching) for such purpose. Our experience
shown that in fact SF6 gives very good results. We use this technique also
in the fabrication of HFET (InP based devices) for the patterning of gate
nitride.

Don't worry Al acts in fact as a etch stop layer.

=20
____________________________________

Mario Caron, M.Sc.A. ing. tel.: (514) 340-3707 fax.: (514)=
340-3706
Associe de recherche fax.: (514) 340-3706
Laboratoire pour l'integration=20
des senseurs et actuateurs
Departement de genie physique =20
Ecole Polytechnique de Montreal =20
C.P. 6079, succ. `centre-ville`
Montreal, Qu=E9bec
H3C 3A7
Web: http://lisa.polymtl.ca





From: shaun.sandow-at-anu.edu.au (Shaun Sandow)
Date: Wed, 12 Jun 1996 15:07:49 +1000
Subject: Re: depassivation of microelectronic components

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Dear All,

Does anyone know of a supplier of synapsin for immunohistochem.?

Thanks,
______________________________________________

Shaun Sandow
Autonomic Synapse Group
Division of Neuroscience
John Curtin School of Medical Research
Australian National University
ACT 0200

email: shaun.sandow-at-anu.edu.au
Ph. (06) 249 4782 (work) (06) 247 6430 (home)
Fax. (06) 249 2687
Email: shaun.sandow-at-anu.edu.au





From: Spectra Services :      mspecht-at-frontiernet.net
Date: Wed, 12 Jun 1996 15:15:22 -0400
Subject: Re: Passivation layer removal

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Here is the web page address for Spectra Services:
http://www.frontiernet.net/~mspecht/
Hopefully, you will find this helfpul. If you have any questions, I can be
reached at 715-654-9500 or by e-mail. Michael Specht
MIKE SPECHT
SPECTRA SERVICES, INC.
1653 East Main Street
Rochester, NY 14609

Visit our Web site at frontiernet.net/~mspecht





From: moxtek-at-MOXTEK.WIN.NET (Clark Turner)
Date: Wed, 12 Jun 1996 10:05:15
Subject: Re: Reconditioned Ion Pumps for Philips TEM's

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X-Mailer: WinNET Mail, v2.51
Message-ID: {89-at-moxtek.win.net}
Reply-To: moxtek-at-MOXTEK.WIN.NET (Clark Turner)
To: bjg-at-uniwa.uwa.edu.au, microscopy-at-sparc5.microscopy.com
CC: cturner-at-moxtek.com

There is a company in Fremont California called Brechtel
Manufacturing. Phone #510-732-9723, Fax #510-732-9153. Their
main business is manufacturing and reconditioning ion pumps. Don't
know if they have worked with this particular pump, but it's
probably worth a try.

D. Clark Turner
Director, Thin Film Products Group
MOXTEK, Inc.
452 West 1260 North
Orem, Utah 84057

phone (801) 225-0930
fax (801) 221-1121
email moxtek-at-moxtek.win.net


}
} } Reconditioned Ion Pumps (SIP's) for Philips EM 400 series TEM's
} }
} } Can anyone out there help with a supplier who can exchange or recondition
} } the ion pump of a Philips TEM. The pumps are made by Edwards, apparently a
} } special for Philips. Model/Code no. EP100/B036-06-000. The pump body would
} } require to be sawn open and rewelded to renew the electrodes.
} }
} }
} }
} }
} Brendon J. Griffin
} Centre for Microscopy and Microanalysis
} The University of Western Australia
} Nedlands, WA, AUSTRALIA 6907
} ph 61-9-380-2739 fax 61-9-380-1087
}
}





From: mxc-at-nucleus.ansto.gov.au (Mike Colella)
Date: Wed, 12 Jun 1996 16:35:54 +0900
Subject: Unsubscribe.

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From: m.colella-at-ansto.gov.au (Mike Colella)
Date: Wed, 12 Jun 1996 16:39:37 +0900
Subject: Subscribe

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From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Wed, 12 Jun 1996 05:55:39 -0500
Subject: TEM: Synapsin for immunohistochem

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Message-Id: {199606120954.FAA00144-at-lihti.org}
Mime-Version: 1.0
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Dear Shaun:

You can obtain human synapsin from:

BIOGENESIS
7 New Fields, Stinsford Road
Poole BH17 0NF, UK
email: biogenesis-at-ltd.co.uk
fax: (1202) 66020

104 Little Mill Road
Sandown, NH 03873, USA
Tel: (603) 887-4600
Fax: (603) 887-4800

Synapsin I (cow brain) is avaiable from:

SIGNAL TRANSDUCTION, INC.
8895 Towne Centre Dr., #105
San Diego, CA CA 92122, USA
Tel: (800) 701-3353
Fax: (714) 492-6790

...another useful nugget dug out of "Lindscott's Directory of Immunological
and Biological Reagents." Hope these help.

Rick Powell

******************************************************************
* NANOPROBES, Incorporated | Tel: (516) 444-8815 *
* 25 East Loop Road, Suite 124 | Fax: (516) 444-8816 *
* Stony Brook, NY 11790-3350, USA | nano-at-mail.lihti.org *
* *
* NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com *
******************************************************************








From: dlietz-at-trentu.ca (deborah Lietz)
Date: Wed, 12 Jun 1996 08:56:04 +0100
Subject: teaching videos and digital equipment

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Thanks to everyone who corresponded to my questions last week. Sorry, for
the duplication and sometimes triplicate of my messages. We just got a new
computer and we've been having problems getting error messages.

Thanks again for your co-operation

Sincerely,
Debbie Lietz

Debbie Lietz
Electron Microscopy Suite
Department of Biology
Trent University
Peterborough, Ontario
K9J 7B8
Telephone: (705)748-1486
Fax: (705) 748-1205
email: dlietz-at-trentu.ca






From: MikGu-at-mme.liu.se (Mikael Gustafsson)
Date: Wed, 12 Jun 1996 12:33:08 +0200
Subject: Time lapse video system

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I would like to know if anyone out there have experience from video time
lapse tape recorders, especially in conjunction w video microscopy.
I4m interested in buying such a system but don't know all alternatives and
their pros and cons. All information of interest.

Thanks in advance



=============================================
Mikael Gustafsson MD, PhD
Dept Med. Microbiology and
Dept Internal Medicine, Cardiology section
University Hospital of Linkoping
S 581 85 LINKOPING
SWEDEN

E-Mail: MikGu-at-mme.liu.se
FAX: 046/13/224789
Phone: 046/13/224783
=============================================





From: Leo Marin :      leo-at-spine.med.utoronto.ca
Date: Wed, 12 Jun 1996 08:51:54 -0400 (EDT)
Subject: Re: frame grabbers

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Dear Elliott,
One source could be "Data Translation". They may be reached at :
fax -------- (508)481 8620
Email------- info-at-datx.com
Internet---- http://www.datx.com

Leo Marin
University of Toronto

On Mon, 10 Jun 1996 ERJ-at-vetmed1.vetmed.ufl.edu wrote:

} Does anyone have any information regarding frame grabbers for Macs? I'm
} looking for something like Snappy which is made for PC's.
} thanks
} Elliott Jacobson
} Professor
} Department of Small Animal Clinical Sciences
} P.O. Box 100126
} College of Veterinary Medicine
} University of Florida
} Gainesville, Florida 32610, USA
} Phone: 904-392-4700 X4773
} Fax: 904-392-6125
} E-Mail: ERJ-at-vetmed1.vetmed.ufl.edu
} WEB Site: www.vetmed.ufl.edu
}
}
}




From: pat_masarachia-at-merck.com (Pat Masarachia)
Date: Wed, 12 Jun 1996 18:42:43 EST
Subject: Core facility- to be or not to be.

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I know this issue has been discussed before and I would be very
appreciative of someone sharing the key ideas with me. We are able
to get money to upgrade our microscopy facility (confocal, EM and LM)
which currently is used exclusively by one dept., if we offer it
as a core facility to all the research groups in this pharmaceutical
research facility. Right now there is one staff person (me) to do EM
and I have my own research projects for the group. What possibility
is there of doing my own research project at the same time as providing
service for others. What is the minimum staff? How does one begin to
judge potential usage? Thanks for your thoughts and my apologies for
addressing issues that may have already been covered.
Pat Masarachia
Merck Research Laboratories
West Point, Pa 19486
phone 215-652-7999
e-mail pat_masarachia-at-merck.com






From: rgronsky-at-garnet.berkeley.edu (Prof. R. Gronsky)
Date: Wed, 12 Jun 1996 11:48:04 -0700
Subject: Summer Council Meeting Liaison report

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Message-Id: {199606121559.LAA00566-at-ns.ge.com}
Microscopy-at-Sparc5.Microscopy.Com

Nestor,

Please feed me with any and all data you'd like me to present to Council as
your liaison. Electronic version is fine. I presume we'll also hear from
you directly during open sessions, but I'll need ammo to be your advocate
during the budget battles.

I'm taking a small family vacation June 13-22 in Pittsburgh (Father's Day
picnic included). But I'll be in all the remaining time until the meeting
in August. Last minute is OK.

Thanks.


****************************************
Ronald Gronsky, Professor & Chair
Materials Science & Mineral Engineering
579 Evans Hall
University of California
Berkeley, California 94720-1760
TEL: (510) 642-3801
FAX: (510) 643-5792
****************************************






From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Wed, 12 Jun 1996 09:14:00 -0400 (EDT)
Subject: Proteoglycans

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Does anyone know of a good staining procedure or processing method for
looking at proteoglycans for TEM studies? I appreciate any help.

Thanks

Phil 8-{)




From: SBDX78A-at-PRODIGY.COM ( KATY T RUSHNOV) (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Thu, 13 Jun 1996 08:09:01 -0500
Subject: Nikon rotatable polarizer

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Does anyone have a 79503, Nikon rotatable polarizer for the old style epi
illuminator? If so please let me know pricing. Thank you, SBDX78A







From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Thu, 13 Jun 1996 08:40:19 EST
Subject: Re: Proteoglycans

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} Does anyone know of a good staining procedure or processing method for
} looking at proteoglycans for TEM studies? I appreciate any help.


Phil,

The technique that I am most familiar with and have had the most
success with uses Ruthenium hexammine trichloride. We did a
comparison between it and the old standby osmium-ferrocyanide method
in growth plate cartilage.
See: Neuhring, Steffens, and Rowland. 1991. Histochemical Journal
23, 201-204.

If you have the equipment available, the best results for retaining
proteoglycans are had with high pressure freezing, followed by
substitution in ethanolic osmium.

Hope this gets you started.


-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Adel :      adel-at-zirc.chem-eng.toronto.edu
Date: Thu, 13 Jun 1996 10:37:02 -0500 (CDT)
Subject: subscrib

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Subscrib





From: Hukee, Margaret J. :      hukee.margaret-at-mayo.edu (Marge Hukee)
Date: Thu, 13 Jun 1996 10:39:07 -0500 (CDT)
Subject: Gold labeling as a service

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Dear List members;
We are interested in communicating with others who are gold labeling as a
service to research or clinical research investigators. We are especially
interested in systematic approaches to multiple antigens, screening
techniques, criteria for project acceptance and other common issues. If
you would like to discuss such issues, please either e-mail me directly or
we can discuss on the listserver. I would be happy to consolidate replies
anonymously and post results on a regular basis. Marge






From: Owen P. Mills :      opmills-at-mtu.edu
Date: Thu, 13 Jun 1996 12:34:47 -0400
Subject: SEM of thermoplastics

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Hello,

A colleague on campus asked me to forward the following message to the
listserver hoping for some useful advice.

} I have encountered some problems in trying to study the morphology of
} thermoplastic modified epoxy and BMI by SEM. The thermoplastics have
} structures similiar to the thermosets, so it is difficult to identify the
} two phases of fractured samples by SEM. We attempted to stain or etch the
} samples. Since there are some unreacted double bonds left in thermoset
} phase, we used OsO4 to stain the samples. However, we thought gold coating
} might cover the effects of stain, so we just looked at uncoated samples at
} low voltage (5kev). We found a lot of electron charging of the surface and
} no images are observable.
}
}
} Does anyone have some experience or suggestion about this? Should I coat
} my sample or use any special conditions or techniques to obtain the images?
} I would very much appreciate it if anyone can give me some ideas about what
} kind of stains or etching reagents might work for my system. The
thermoplastics } all have aromatic rings, nitrogens and carbonyls if that
makes a difference?
}

Thanks in advance for any assistance.

Regards

Owen
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: Richard Thrift :      Richard_Thrift-at-depotech.com
Date: Thu, 13 Jun 1996 12:15:38 -0800
Subject: Can deconvolution be used for ratiometric ion

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Message-Id: {s1c00680.076-at-depotech.com}
X-Mailer: Novell GroupWise 4.1

concentrations?

This was sent to the Confocal listserver but got no response; perhaps
the microscopy group has had more experience.

HI folks
I'm interested in determining ion concentrations ratiometrically (specifically
pH, around pH 3-7 so probably using NERF dyes) at the best resolution
possible with merely reasonable effort. I have not done this yet & don't
really know what is involved. I wonder if anyone has experience with
ion concentrations using deconvolution methods; does it work well, how
accurate, are there particular pitfalls to watch out for, etc. Comments
regarding confocal, or comparisons, are also welcome.

Just checked my notes; I've heard from a user of Deltavision, for calcium
(sounds very impressive). Have other deconvolution systems given good
results?

Thanks very much
Richard Thrift

Richard_Thrift-at-Depotech.com
DepoTech Corp
10450 Science Center Drive
San Diego Ca 92121 USA






From: GeneXs-at-aol.com
Date: Thu, 13 Jun 1996 15:40:18 -0400
Subject: Catalese problems

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Greetings everyone:
We recently purchased a Catalese TEM grid from a commercial supplier. It was
to be used to check-out the resolution of our two microscopes (a Zeiss
EM109, and Siemens 101). We had difficulty seeing any periodicity at all
in the Siemens, although we searched for about one hour. The Zeiss
revealed photographable and measure periodicity in approximately 1 in 100
crystals. We returned the grid to the Siemens, but still could not fined any
usable crystals. Before we determined the Seimens had poor resolution, we
returned the grid to the Zeiss--but to our shock, surprise, chagrin, and
consternation--could find *no* good crystals!
Questions:
1) Are these crystals sensitive to damage from the beam (like contamination)?
2) Would a carbon coat benefit catalese grids purchased in the future?
3) For that matter, is there is anything better than catalese?
Thanx
cya,
Gene




From: pulcheri-at-geo.Princeton.EDU (Pulcherie Gueneau)
Date: Thu, 13 Jun 1996 15:05:36 -0400
Subject: Seeking advice on avoiding major pitfalls in elemental microanalysis of thin-sections of biological material

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If anyone has experience in elemental microanalysis on thin sections of

BIOLOGICAL material, we would very much appreciate a piece of advice, to avoid

the major first-try mistakes.



Material :

Microalgae, (=particles 10 um diameter), embedded in Spurr's or in LR white resins, after being fixed in glutaraldehyde (2.5%) and reduced OsO4. Thin sections are 80 nm.

Goal :

Analyze the elemental composition of certain structures seen on the thin sections, in particular, those containing IRON, like ferritin (dense iron core, about 10 Angstroms).

Instrumentation :
we have all the equipment and experience necessary for analysis of mineral material

FEG-TEM with EDS and EELS, EPMA with WDS, etc.


Please reply to : PULCHERI-at-GEO.PRINCETON.EDU





Pulcherie GUENEAU
Francois Morel group
Guyot Hall- Geology
Princeton University
Princeton NJ 08544-1003 tel 1.609.258.5746
Fax : 1274
USA






From: John Millar :      jjmill-at-rmit.edu.au
Date: Fri, 14 Jun 1996 10:52:48 EST-10
Subject: Used SEM : JEOL 35 CF

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To the list :

JEOL 35CF available for sale. Fully operational and the subject of
many years TLC. Still a very good machine, but space requires its
departure. Photo output has been digitised to provide 1024x1024 high
quality files directly readable by standard programs such as P-MAN.
Analogue output has been retained as well.
Enquiries to and further details from
Professor John J. Millar, PhD
Department of Applied Physics and
Electron Microscope Unit
RMIT, GPO Box 2476V, MELBOURNE AUSTRALIA 3001
+613 9660 2602 fax 613 9660 5290
email jjmill-at-rmit.edu.au




From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Fri, 14 Jun 1996 12:28:27
Subject: Re: Vibration isolator

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To: microscopy-at-Sparc5.Microscopy.Com

In article Takanori Maeda {maeda-at-crdl.pioneer.co.jp} writes:
} From: Takanori Maeda {maeda-at-crdl.pioneer.co.jp}
} Subject: Vibration isolator
} Date: Thu, 13 Jun 96 10:06:50 JST

} Hello.

} We are going to buy Leitz DMRM microscope with a 150X objective lens.
} The salesperson told me that an air-suspentioned vibration isolation
} table is not necessary for Leitz microscopes or even halmful.
} We have space available both on an insulation table and on an ordinary
} table. ( It looks rigid enough.)
} I appreciate if you tell me your experiences about the insulation
} especially with high magnitude lenses.

Cheap vibration checker:- place a shallow dish of water on the bench you want
to check for vibration. Then look at the reflection of a light source in the
water. It should be quite still.

Cheap vibration isolator:- get a paving slab - here we can buy cement slabs
about 500 mm square x 50 mm thick OR a slab of marble OR stone. Anything
dense and heavy that is large enough to accommodate the microscope. Put 4
tennis balls under the 4 corners of the slab. OR the inner tube from the tyre
of a small car or motor cycle a bit smaller thasn the slab. If you get the
valve relocated on the OUTside of the tube you can re-inflate it without
moving everything. Put the microscope on the slab.

Mel Dickson





From: Sara Prins :      SPrins-at-csir.co.za
Date: Fri, 14 Jun 1996 11:54:58 +0200
Subject: subscribe

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Disclaimer: The views expressed in this message are those of the
author, and not necessarily those of the CSIR and/or
it's employees.
Message-Id: {s1c15319.061-at-csir.co.za}
X-Mailer: Novell GroupWise 4.1

I would like to subscribe to this newsgroup as I recently
became involved in the electron microscopes at Mattek,
CSIR.
Thank You
Sara Prins





From: Kukin V.N. :      lemi-at-mx.iki.rssi.ru
Date: Fri, 14 Jun 1996 16:08:37 +0400 (MSK)
Subject: New SEM submicron measurement technique

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To attention of all connected with SEM measurements of line di-
mensions in the micron, submicron, nanometer ranges.

New technique of high precision and accuracy measurements of line
dimensions for the submicron and nanometer ranges has been developed
by us. This technique is based: 1. on the solution of the main prob-
lem of metrology: the problem of the location of edges of objects in
images and 2. on the calibration of the SEM magnification with the
help of the pitch standards.
Testing measurements have demonstrated that the technique secu-
res the accuracy of measurements better than 5 nm in the dimension
range of 100 - 500 nm.
Other advantages of the technique
It gives the reliable metrological maintenance for measurements
in the submicron and nanometer ranges.
It provides the highest precision among all known techniques of
SEM measurements of line dimensions.
The measurement precision does not depend on an object material.
It is based on an use of cheap and available pitch standards.
It does not require linewidth standards during of routine measu-
rements.
It can be easy automatized and computerized and in this variant
it does not require the personnel of high skill.
It can be used for step-by-step operation checking in conditions
of mass production.
It can be developed to measurements of features of complicated
relief (non-rectangular in the cross-section) or alternate layers
of different materials.
We have also ideas about specific improvements of SEM, allowing
to realize our technique in the automatized mode.
In the simple variant the technique can be organized in your la-
boratory during 1 month.
We are ready elaborate our technique to your specific require-
ments.
You can get an additional information, if you contact with
Prof. A.Nikitin or Prof. S.Maksimov, Joint Center for Fundamental
Problem in Microelectronics of the Russian Academy of Sciences at
Moscow Institute of Electronic Technology, Moscow, 103498, Russia.
Our E-mail lemi-at-mx.iki.rssi.ru.

With the best wishes.
Sincerely Yours.
S.Maksimov.





From: Donald Lovett :      lovett-at-trenton.edu
Date: Fri, 14 Jun 1996 08:31:27 -0400 (EDT)
Subject: Re: Vibration isolator

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I had purchased an air-suspension type vibration isolation table
(brand name withheld intentionally) for my research microscope.
Even though the sales person explained that this was exactly what I
wanted, it did not meet my needs. Once the system is suspended, one
cannot touch the microscope, or else it takes considerable time for it to
stop rocking back and forth. Although we could purchase a remote shutter
release to solve part of the problem, our real problem is that *any*
adjustment (stage movement, focus) causes considerable motion in the
scope. We have had to use the table with the suspension system
disengaged. The table is useful in that its frame is made of a
heavy steel construction and the work surface is a 2-inch thick composite
slab, which takes two people to lift. The heavy weight of the unit does
help reduce vibration, but at a cist if $4,000 I bet we could have spent our
money in better ways.

If someone out there has recommendations, I, too, would like to hear them.

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-trenton.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
Trenton State College, NJ 08650-4700 fax: (609) 771-2674







From: dlietz-at-trentu.ca (deborah Lietz)
Date: Fri, 14 Jun 1996 09:52:40 +0100
Subject: responses to teaching videoes and digital equipment

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For those of you who are interested the MSA has a 12 page listing of
video tapes which can be purchased. The list can be obtained by going to
the MSA web page at http://www.MSA.Microscopy.com. Other possibilities for
tapes although somewhat biased are tapes from manufacture's of equipment.
Several people indicated that Reichart put out a good tape several years
ago about block trimming and sectioning. I'm trying to see if it is still
available. I haven't got around to it yet but try other microscopy
societies.

As to digital equipment the number of companies is quite long and it
seems everyone has different needs etc.. Most of the large microscope
companies carry some kind of system and you can get a lot of names off the
net. I haven't looked into all the companies that have been suggested but
to simplify things a bit the following are the systems we are going to
start with:

SEMICAPS 1000
HITACHI QUARTZ PCI
4Pi
Leica Lida Image Database

Remember these are our choices to start with but there are lots of other
companies around so check them out. As to cameras and printers that is
something else entirely. I'll try and add some input after we have seen
the companies' demos. Don't forget those of you going to the MSA
conference will get to see most of the systems at work.

Now that people know what companies we are starting with I'm still looking
for feedback either pro or con. Thanks

I have no vested interest in any of the companies mentioned.

Sincerely,

Debbie Lietz

Debbie Lietz
Electron Microscopy Suite
Department of Biology
Trent University
Peterborough, Ontario
K9J 7B8
Telephone: (705)748-1486
Fax: (705) 748-1205
email: dlietz-at-trentu.ca






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Fri, 14 Jun 1996 08:42:39 -0500
Subject: Re: Seeking advice on avoiding major pitfalls in elemental

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Message-Id: {199606141444.JAA27446-at-watson.bcm.tmc.edu}
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microanalysis of thin-sections of biological material
Cc: microscopy-at-Sparc5.Microscopy.Com

At 03:05 PM 6/13/96 -0400, you wrote:
}
} If anyone has experience in elemental microanalysis on thin sections of
}
} BIOLOGICAL material, we would very much appreciate a piece of advice, to avoid
}
} the major first-try mistakes.
}
}
***********************
Pulcherie GUENEAU -

I would be very much interested in participating in this exchange. Would
you, and your correspondants, be willing to cc to the listserver?

I have a bit of experience identifying lanthanum in thin sections of Spurr
embedded rat lung. Basically I collected thin sections on berylium grids and
put them in graphite grid holders. I located the heavy La by backscatter
imaging and then probed at the lowest kEv that I had (20 kEv). From the
resulting spectrum I stripped a spectrum of an empty Spurr section. This was
done using a JEOL 100-C TemScan fitted with a Tracor-Northern TN-5500. As I
recall, we then probed Pb/U stained sections in the same way and found that
the Pb and U peaks did not interfere with the La peaks.

Joiner

Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: nano-at-ns1.LIHTI.ORG (Nanoprobes Inc.)
Date: Fri, 14 Jun 1996 11:18:32 -0500
Subject: Re: Gold labeling as a service

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Mime-Version: 1.0
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Dear Marge:

Nanoprobes offers custom gold labeling. You can also obtain our gold
cluster reagents (undecagold and Nanogold) in reactive forms (maleimido-,
sulfo-NHS, amino-) with which you may label biomolecules in your own
laboratory.

While I'm here, our WWW site is now completely up and running at

http://www.nanoprobes.com

including our complete catalog and info on custom labeling.

******************************************************************
* NANOPROBES, Incorporated | Tel: (516) 444-8815 *
* 25 East Loop Road, Suite 124 | Fax: (516) 444-8816 *
* Stony Brook, NY 11790-3350, USA | nano-at-mail.lihti.org *
* *
* NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com *
******************************************************************






From: Phil Elizondo :      PELIZONDO-at-svc.com
Date: Fri, 14 Jun 1996 10:38:31 PST
Subject: Optical microscope rental/leasing

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Fellow microscists,

I wonder if there are any resources in the U.S. that offer first rate
top of line optical microscopes for rental/leasing. Are purchase cost to low
for any reasonable rental prices? We are interested in a possible
lease with option to purchase plan as well. Any help would be greatly
appreciated. Thank You!


Phil Elizondo
Res. Engr.
SVC




From: opmills-at-mtu.edu_at_internet at x400post
Date: 6/13/96 11:34 AM
Subject: SEM of thermoplastics

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---------------------------- Forwarded with Changes ---------------------------
Gold is great for fracture morphology examination, but as
you noted, will seriously hurt your signal/noise (contrast)
for BSE work. Don't know what incident beam potential
would result in charge equilibrium for this material(s).
Probably much lower than 5kV. Below 3-5kV, many diode BSE
detectors do not work well. A scintillator (Robinson) type
would be needed to view chemistry differences. On a fracture
surface, topographical effects can easily mask small atomic
number variations.

Try evaporating carbon for a conductive film. This will let
you use 5-10kV without disruptive charging. If the analysis
requirements permit, a carbon coated polished sample would
enable you to see (BSE) the smallest atomic number changes.

Woody - woody.n.white-at-mcdermott.com
Babcock & Wilcox Research Div. (LRC)


______________________________ Forward Header __________________________________


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A colleague on campus asked me to forward the following message to the
listserver hoping for some useful advice.

} I have encountered some problems in trying to study the morphology of
} thermoplastic modified epoxy and BMI by SEM. The thermoplastics have
} structures similiar to the thermosets, so it is difficult to identify the
} two phases of fractured samples by SEM. We attempted to stain or etch the
} samples. Since there are some unreacted double bonds left in thermoset
} phase, we used OsO4 to stain the samples. However, we thought gold coating
} might cover the effects of stain, so we just looked at uncoated samples at
} low voltage (5kev). We found a lot of electron charging of the surface and
} no images are observable.

Regards

Owen
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu




From: bozzola-at-siu.edu (John J. Bozzola)
Date: Fri, 14 Jun 1996 13:12:56 -0600
Subject: LM:immuno-reactivation w microwaves

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A colleage is interested in the possibility of the reactivation of
antigenic reaction sites in specimens that have been fixed in cold 2%
formaldehyde. They read somewhere that microwaves could be used to
reactivate antigenic sites that were somehow non-reactive to some
antibodies. Does anyone have any info on this subject. If so, please
contact: mparr-at-somc.siu.edu. Thanks.

#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: John Best :      jbest-at-vicon.net
Date: Fri, 14 Jun 1996 09:10:25 -0700
Subject: Re: SEM of thermoplastics

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Message-ID: {31C18EF1.4338-at-vicon.net}

Owen P. Mills writes:

=====================================================================
I have encountered some problems in trying to study the morphology of
thermoplastic modified epoxy and BMI by SEM. The thermoplastics have
structures similiar to the thermosets, so it is difficult to identify the
two phases of fractured samples by SEM. We attempted to stain or etch
the samples. Since there are some unreacted double bonds left in
thermoset phase, we used OsO4 to stain the samples. However, we thought
gold coating might cover the effects of stain, so we just looked at
uncoated samples at low voltage (5kev). We found a lot of electron
charging of the surface and no images are observable. Does anyone have
some experience or suggestion about this? Should I coat my sample or use
any special conditions or techniques to obtain the images? I would very
much appreciate it if anyone can give me some ideas about what kind of
stains or etching reagents might work for my system. The thermoplastics
all have aromatic rings, nitrogens and carbonyls if that makes a
difference?
=====================================================================
My suggestion:
Sorry if my advice is so general, but I'm not a chemist! I'll make two
suggestions anyway.

1. I suggest that you look at the chemistry of the phases and see if
there is a difference that would allow you to selectively react one of
the phases in a way that would allow it to pick up a quantity of a
heavier element. You might then be able to image the difference in
phases using a BSE system in atomic number contrast mode.

2. Same as one except use elemental mapping with an EDS. Instead of
needing enough of a heavier material for atomic # contrast, you'll need
enough material that is detectable with your present EDS detector. This
will give you a wider variety of choices for the reaction you can use.

I would think the OsO4 would have provided (depending on the quantity
attached) would have worked. Why son't you give it another try with a
very thin carbon coating and play with the accelerating voltage to blast
through the coating and excite the Osmium underneath?

Regards,

John Best.






From: Hukee, Margaret J. :      hukee.margaret-at-mayo.edu (Marge Hukee)
Date: Fri, 14 Jun 1996 13:46:02 -0500 (CDT)
Subject: gold labeling-2

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Dear list members;
After receiving some replies to my "feeler" about gold labeling as a
service, I think we can expand this discussion further. A common complaint
is the low success rate of labeling especially with monoclonals, with most
"paying" customers unwilling to try the procedure more than 1 or 2 times
before abandoning the project. Maybe a solution to this complaint would be
to improve our screening techniques prior to embedding tissue. What
criteria (if any) are required before IEM is done on a particular sample??
My first step is a literature search that emphsizes the technical nature
of the project. We would like to see western blots and light microscopy
results, but few of our investigators have the facilities to do these prior
to IEM. What screening techniques are necessary or are done on a routine
basis by others doing multiple antigens?? Again if you wish to remain
anonymous, e-mail me directly and I will summarize replies. Later
Marge






From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Fri, 14 Jun 1996 17:13:08 -0400 (EDT)
Subject: Re: LM:immuno-reactivation w microwaves

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Check out recent article by Clive Taylor in Cell.

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************





From: bozzola-at-siu.edu (John J. Bozzola)
Date: Fri, 14 Jun 1996 16:03:31 -0600
Subject: Re: LM:immuno-reactivation w microwaves

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CORRECTION:
IN AN EARLIER REQUEST,BELOW, I LISTED THE WRONG RETURN ADDRESS FOR DR PARR.
THE CORRECT ADDRESS IS mparr-at-som.siu.edu
VERY SORRY.


A colleage is interested in the possibility of the reactivation of
antigenic reaction sites in specimens that have been fixed in cold 2%
formaldehyde. They read somewhere that microwaves could be used to
reactivate antigenic sites that were somehow non-reactive to some
antibodies. Does anyone have any info on this subject. Thanks.

#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Neal Nicklaus :      nnicklaus-at-nova.sarnoff.com
Date: Fri, 14 Jun 1996 18:28:11 -0400
Subject: Mounting Media for Living Samples

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I have recently started working with "living" samples and need suggestions
on what to use to seal my cover slips to the microscope slide. I have been
using acrylic nail polish, but it has been suggested that the acetone might
be getting into the samples and changing the biochemistry. I am trying to
visualize enzyme action on strands of dna in a fluorescence microscope.

Any suggestions or references would be appreciated.



P.S. My background is primarily physics & engineering, so I am not use to
biology or biochemistry samples.

Neal Nicklaus

SEQ Limited

Voice: 609-452-6033 Ext. 13
Fax: 609-452-5955
email nnicklaus-at-seq.sarnoff.com





From: Jill Craig :      jcraig-at-unbc.edu
Date: Fri, 14 Jun 1996 16:31:38 -0700 (PDT)
Subject: SEM output - cameras, videoprinters, other printers

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Hi all,

I am looking for information/recommendations on output devices. We have
a Polaroid 4X5 pack film back and capacity to save images as .img and .tif.

We are currently considering a Sony videoprinter for its fast, inexpensive
images to be used for working shots and either a digital or high quality
laser printer for material to be published or used in presentations.

Our work load is extremely varied with some emphasis on metals or soils.
Adobe photoshop has been recommended as the industry standard software.

Also, we are considering buying standards for calibration of Edax,
backscatter detector, etc. There appears to be quite an array of very
expensive options. What ones are indispensable and when do you use them?


Any and all information will be greatly appreciated.

Thanks,

Jill




From: Michael Nesson :      nessonm-at-ava.bcc.orst.edu
Date: Fri, 14 Jun 1996 14:18:29 -0700 (PDT)
Subject: Re: LM:immuno-reactivation w microwaves

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John:
Here are a few pertinent references:
1. Shi,S. et al.
Antigen retrieval in formalin-fixed parrafin-embedded tissues:.......
J.Histchem.Cytochem.39:741-748 (1991).

2. Taylor,CR et al.
Strategies for improving the immunohistochemical staining of...
Hum.Pathol.25:263-270 (1990).

3. Momose H. et al.
Antigen retrieval by microwave irradiation in lead thiocyanate....
Appl.Immunohistochem.1:77-82 (1993).

4.Leong,A.S-Y. & J. Milios
An assessment of the efficacy of the microwave antigen-retrieval...
Appl.Immunohistochem.1:267-274 (1993).

5. Cuevas,E.C. et al.
Microwave antigen retrieval in immunocytochemistry: A study of 80 Abs.
J.Clin.Pathol.47:448-452 (1994).

6. Gown,A.M. et al.
Microwave-based antigenic unmasking......
Appl.Immunohistchem.1:256-266 (1993).

I haven't tried any of the above personally; the references are all taken
from a review article in Cell Vision 1(4): ?-288 (1994) by Leong.

Good luck!
Mike--


_______________________________________________________________________
Michael Nesson nessonm-at-bcc.orst.edu (541)737-1866 FAX:(541)737-0481
Dept. of Biochem/Biophys., 2011 AgLS, Oregon State University
Corvallis, OR 97331-7305





From: Jeff L. Brown :      brown-at-el.wpafb.af.mil
Date: Fri, 14 Jun 1996 17:13:35 -0400
Subject: Vibration isolator

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Donald L. Lovett wrote:

I had purchased an air-suspension type vibration isolation table
(brand name withheld intentionally) for my research microscope.
Even though the sales person explained that this was exactly what I
wanted, it did not meet my needs. Once the system is suspended, one
cannot touch the microscope, or else it takes considerable time for it to
stop rocking back and forth. Although we could purchase a remote shutter
release to solve part of the problem, our real problem is that *any*
adjustment (stage movement, focus) causes considerable motion in the
scope. We have had to use the table with the suspension system
disengaged. The table is useful in that its frame is made of a
heavy steel construction and the work surface is a 2-inch thick composite
slab, which takes two people to lift. The heavy weight of the unit does
help reduce vibration, but at a cist if $4,000 I bet we could have spent our
money in better ways.

If someone out there has recommendations, I, too, would like to hear them.

**********************

The only time I have ever experienced anything like you describe here is
when I mounted a large interferometric microscope onto a relatively small
vibration isolation platform knowing full well that the center of mass of
the microscope would likely fall outside of what was recommended for the
isolation table. In circumstances like this, the leveler arms which control
the valves to add or bleed air as necessary cannot respond properly and the
table oscillates, sometimes violently. I suspect that this is your problem
or that there is simply a malfunction or defect in your isolation system.

All the micrscopes I use are vibration islolated with pneumatic vibration
isolation legs and none are as touchy as you describe. An air table should
settle down quickly when rocked and should have enough damping so that
normal adjustments to the microscope (or whatever is being isolated) do not
cause any rocking at all. A properly sized pneumatic vibration isolation
table can easily accomplish this.

Good luck!






Jeff L. Brown
WL/AADP BLDG 620 brown-at-el.wpafb.af.mil
2241 Avionics Circle RM C2G69 ph: (513) 255-4736
Wright-Patterson AFB, OH 45433-7322 fax: (513) 255-3374





From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Fri, 14 Jun 1996 21:51:51 -0500
Subject: Re: Mounting Media for Living Samples

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Greetings,
Neal Nicklaus wrote:
} I have recently started working with "living" samples and need suggestions
} on what to use to seal my cover slips to the microscope slide. I have been
} using acrylic nail polish, but it has been suggested that the acetone might
} be getting into the samples and changing the biochemistry. I am trying to
} visualize enzyme action on strands of dna in a fluorescence microscope.
}
Well, your physics background will smile but an old stand by for
this sort of thing is "valap", which is a mixture of equal weights of
vasoline, lanolin and paraffin. (I am *not* making this up). You put the
ingredients together in a beaker and then heat gently. They melt and you
mix well. The stuff will cool and solidify and be good for years. When you
want to seal a slide, you heat it up again gently and apply a filet to the
coverslip, just like nail polish. It won't make super permanent slides, but
it will give you a day or two of life under the scope. Hope this helps,
Tobias Baskin
PS. Do I need to mention that I am have financial ties to the manufacture
of neither vasoline, nor lanoline nor paraffin?

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / 1623 University Ave.
/ | / / \ / / Columbia, Missouri
/___ / /__ /_____\ / /__ 65211 USA
/ / / \ ( /
/ / / \ \ / voice: 573 - 443-1984
/ /____ / \ \____/ /_____ fax: 573 - 882-0123






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Fri, 14 Jun 1996 20:11:05 -0800
Subject: Re: current ISI phone number or address

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Dear Yolande,
ISI became Topcon a few years ago. They have ads in Microscopy Today and
JMSA and have a toll-free number at 1-800-538-6850. I know they still do
TEMs and SEMs, so I assume they carry coaters as well.
Regards,
Mary
} Hello,
}
} Is ISI still in business, and do they have a new phone number?
} We have a ISI sputter coater that needs repair, and we would like to get in
} touch with them.
}
} Yolande Berta
} School of Materials Science and Engineering
} Georgia Institute of Technology
} 778 Atlantic Dr.
} Atlanta, GA 30332-0245
} (404)894-2545
} yolande.berta-at-mse.gatech.edu

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Marc C. Brande, MS, Founder (619) 587-4830 FAX: (619) 552-1516 :      mcbrande-at-sierra.net
Date: Sat, 15 Jun 1996 09:10:10 +0000
Subject: Live-Cell Imaging Facilities ?

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Message-Id: {31C27DF2.6A4C-at-sierra.net}
microscopy-at-Sparc5.Microscopy.Com, neur-sci-at-net.bio.net,
nih-image-at-soils.umn.edu

Is there a directory of facilities (academic/commercial) providing
Live-Cell Imaging/Microscopy for a fee? (USA/World). I have clients in
need of such a service. Any information would be much appreciated.

Marc




From: Steven W. Miller :      73150.2217-at-CompuServe.COM
Date: 16 Jun 96 13:00:55 EDT
Subject: Air Table Oscillations

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Message-Id: {m0uUxOU-0004BFC-at-fast.net}

As a supplier and installer of air tables I would like to add one piece of
information to the string.

The recommended air pressure in the specifications with tables is sometimes as
high as 80psi.
I don't think I have ever seen an installation that worked well at these high
pressures (with ultramicrotomes and other units under 500 lbs).

When installing I try to balance the pressure such that there is only a quarter
of an inch maximum of space between the frame and table top, this limits the
travel and prevents dangerous tilting. The higher the bladders are filled the
more unstable the motion.

Secondly, remember that the pressure equates to the speed of the response when a
valve is opened so I usually end up with a pressure of 40PSI or slightly higher.
This will also change the characteristics of the table's vibration response so
be sure to check the isolation when changing pressures.

Lastly, with very small systems the suspended mass is awfully low and adding
some mass to the table may help. Chunks of iron or lead work well, get it in
three pieces to allow balancing the top.

Don't hesitate to call the manufacturer for help.

Steve Miller
Integrated Microsystems, Inc.
Park Ridge, IL
Phone 800-388-8801






From: Charles A. Garber :      103532.3325-at-CompuServe.COM
Date: 16 Jun 96 16:41:06 EDT
Subject: SEM visualization of stearates

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On June 6, Tania Jones wrote the following:
========================================
} our lab is trying to find a way to detect very thin ( {1um thick) coatings
} of stearates on the surface of a galvanized wire. our equipment consists
} of only a sem and a standard edx (no light element). any suggestions on
} how to "see" the coating would be very helpful.
========================================

Over the years, the only time we have been able to "detect" the presence of
stearates on a surface of this type was by replication TEM, using Pt/C
techniques. These were samples where there really was not a continuous coating,
but a scattering around of small (less than 100 nm) acicular shaped "crystals"
with aspect ratios larger than 3:1. Now I can't swear the samples we have seen
are representative of the world, but we have ourselves never seen such stearate
coatings where there actually was a continuous coating and one that could be
imaged by SEM. The only time we have seen anything by SEM turned out to have
the appearance of being "globs" but that was not the usual situation. Assuming
these are metal strearates of one type or another, at least several instances I
can recall, the stearate crystals could be stripped off with the replica thereby
remaining with the replica, and with EDS (no light element capability) we could
confirm the presence of the metal connected to the stearate. So the analysis of
such samples consisted of a combination of EDS and morphological observations.
You did not mention a wire diameter, but since we have replicated wires
approaching the diameter of a human hair wire diameter should be no problem.

One in theory can embed and diamond knife thin section the wire and attempt to
image the crystals in cross section. However, again, if you coatings are
typical of the ones we have seen, "seeing" the crystals could be very difficult,
complicated in part because of the differences in hardness between the wire and
the surrounding embedding resin. Now we have actually done this, but it is a
whole lot less satisfying that the surface replication approach (based on our
own experience).

Disclosure: Our firm does this type of TEM work as a laboratory service for
clients and would therefore have a vested interest in someone doing this kind of
a study by TEM instead of by SEM.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.com

Look us up!
###########################
WWW: http://www.2spi.com
###########################
======================================================





From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Sun, 16 Jun 1996 22:50:53 -0400 (EDT)
Subject: Re: Mounting Media for Living Samples

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To seal living samples, VALAP (equal mixture of vaseline, lanolin, and
parafin works well. It melts at low temperature, so it will not heat the
slide and sample when applied. It forms a watertight seal. You can make
the slide permanent later by perfusing in glutaraldehyde by capillary
action (poke small hole at either end of seal and use filter paper at one
end to draw fluid through the sample) and then sealing coverglass with
nail polish over the valap.
This has worked well for us in live cell experiments.

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************

On Fri, 14 Jun 1996, Neal Nicklaus wrote:

} I have recently started working with "living" samples and need suggestions
} on what to use to seal my cover slips to the microscope slide. I have been
} using acrylic nail polish, but it has been suggested that the acetone might
} be getting into the samples and changing the biochemistry. I am trying to
} visualize enzyme action on strands of dna in a fluorescence microscope.
}
} Any suggestions or references would be appreciated.
}
}
}
} P.S. My background is primarily physics & engineering, so I am not use to
} biology or biochemistry samples.
}
} Neal Nicklaus
}
} SEQ Limited
}
} Voice: 609-452-6033 Ext. 13
} Fax: 609-452-5955
} email nnicklaus-at-seq.sarnoff.com
}
}




From: Timon.Fliervoet-at-uni-bayreuth.de (Timon Fliervoet)
Date: Mon, 17 Jun 1996 10:07:21 +0100
Subject: talking about electro-polishing

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Dear all,

To continue the discussion on electro-polishing of Mg. Does any of you know
the polishing agents and etchants used for electrolytically polishing
Lead-Tin alloys to TEM sections.

Thanks

---------------------
Dr. Timon Fliervoet
Bayerisches Geoinstitut, Universitat Bayreuth
D-95440, Deutschland
tel: ++49 921 553745; fax: ++49 921 553769






From: ebs-at-ebsciences.com
Date: Mon, 17 Jun 1996 07:09:15 -0500
Subject: Re: current ISI phone number or address

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Yolande,

ISI is still very much in business, as Topcon Technologies, Inc. They can
be reached at 201-261-5410 (phone) or 201-262-1504 (fax).

However, the ISI sputter coaters were made by Polaron, and private-labelled
for ISI. As far as I know, Topcon does not service them. Polaron is now
part of VG Microtech, and we (Energy Beam Sciences) are VG's authorized
service agent in the United States. We have spare parts in stock for all
models of Polaron/BioRad/ISI sputter coaters, and technicians trained by VG
to repair them.

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: ebs-at-ebsciences.com
Date: Mon, 17 Jun 1996 07:03:29 -0500
Subject: LM:immuno-reactivation w microwaves

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} } From microscopy-request-at-sparc5.microscopy.com Fri Jun 14 21:04:20 1996
} X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
} Date: Fri, 14 Jun 1996 13:12:56 -0600
} To: microscopy-at-aaem.amc.anl.gov
} From: bozzola-at-siu.edu (John J. Bozzola)
} Subject: LM:immuno-reactivation w microwaves
}
} A colleage is interested in the possibility of the reactivation of
} antigenic reaction sites in specimens that have been fixed in cold 2%
} formaldehyde. They read somewhere that microwaves could be used to
} reactivate antigenic sites that were somehow non-reactive to some
} antibodies. Does anyone have any info on this subject. If so, please
} contact: mparr-at-somc.siu.edu. Thanks.
}
We have several good reference articles on microwave techniques for antigen
retrieval. If you'll e-mail me your snail mail address, I'll be happy to
send them to you.

Best regards,
Sonja L. White, Sales & Marketing Secretary
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: Gary Login :      glogin-at-bih.harvard.edu
Date: Mon, 17 Jun 1996 08:42:10 -0400
Subject: Re: Antigen Retrieval

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microscopy-at-aaem.amc.anl.gov

Daryl, I recommend contacting Dr. Shi at {shi-at-hsc.usc.edu} . My understanding of
Dr. Shi's take home message from a recent workshop was that most antigens can be
retrieved from formaldehyde tissue heated between 95-100¡C- for 20-30 minutes-
at pH 1-2 (the buffer or salt solution is not important). In fact acid or base
pH is superior to neutral pH for most tissue sections.

Gary Login.



In message writes:
} Hello,
}
} I was wondering if I could ask for your help? We are getting ready
} to start to do Antigen Retrieval with Citrate Buffer. We have read
} many journals talking about the procedure. I was wondering if you
} would email, fax, or send me a copy of your procedures for doing the
} Citrate Buffer Antigen Retrieval. The pathologists want me to get
} some procedures,so we can get a better prospective of the procedures
} used at other hospitals. I would appreciate it.
}
}
} Thanks,
} Daryl A. Mikita, HT(ASCP)
}
} Wyoming Medical Center
} Department of Pathology
} 1233 E. 2nd St.
} Casper, WY 82601
}
} Voice: (307) 577-2198
} Fax: (307) 577-2396
}
} Email: daryl_mikita-at-wmc.ccmail.compuserve.com
} WWW: http://www.wmcnet.org/pathology
}


Gary R. Login, D.M.D., D.M.Sc.
Assistant Professor of Oral Pathology
Beth Israel Hospital
Department of Pathology
330 Brookline Avenue
Boston, Massachusetts, 02215

glogin-at- bih.harvard.edu
Telephone: 617-667-2034
Fax: 617-667-8676





From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Mon, 17 Jun 1996 09:17:14 -0400
Subject: Re: SEM output - cameras, videoprinters, other printers

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In recent months, many questions such as this have been posed. So
many in fact that it spurred us to begin archiving the responses.The digital
revolution is now in full swing and I now have quite an extensive list of
discussions. Look at the web address at the end of this message and follow
the link to "Tips & Tricks". You will find most of the info you are looking
for in the Computer applications section. Let me know if you cannot access
the info and I wil send it to you directly.



At 04:31 PM 6/14/96 -0700, you wrote:
}
} Hi all,
}
} I am looking for information/recommendations on output devices. We have
} a Polaroid 4X5 pack film back and capacity to save images as .img and .tif.
}
} We are currently considering a Sony videoprinter for its fast, inexpensive
} images to be used for working shots and either a digital or high quality
} laser printer for material to be published or used in presentations.
}
} Our work load is extremely varied with some emphasis on metals or soils.
} Adobe photoshop has been recommended as the industry standard software.
}
} Also, we are considering buying standards for calibration of Edax,
} backscatter detector, etc. There appears to be quite an array of very
} expensive options. What ones are indispensable and when do you use them?
}
}
} Any and all information will be greatly appreciated.
}
} Thanks,
}
} Jill
}
}




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/





From: Luc Nocente :      ln-at-noesisvision.com
Date: Mon, 17 Jun 1996 13:28:51 -0400
Subject: Question on Visilog from Dr.Misra

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X-Sender: ln-at-noesisvision.com
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Dear Dr. Misra from Unilever Research, you sent me a question on Visilog the
other day, unfortunately your email number does not appear on the mail and I
therefore can not respond to your message. IF you could send me your email
number, I can then respond to your message.

----------------------------------------------------------
---------------------------------------
Luc Nocente Tel: 514 345 1400
Noesis Vision Inc. Fax: 514 345 1575
e-mail: ln-at-noesisvision.com
6800 Cote de Liesse, Suite 200
St-Laurent, PQ
H4T 2A7,Canada

Visit our new web site at http://www.cam.org/~noesis
----------------------------------------------------------------------------
---------------------





From: Delilah W. Irving :      dirving-at-aggie.pw.usda.gov
Date: Mon, 17 Jun 1996 12:08:26 -0700 (PDT)
Subject: Oxford U.S. telephone

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Does anyone happen to have the address and phone number for Oxford
Instruments, Ltd...they make cryo stages.

Thank you

Delilah W. Irving tel: 510-559-5653
USDA - ARS - WRRC fax: 510-559-5777
800 Buchanan St. email: dirving-at-pw.usda.gov
Albany, CA 94710






From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Mon, 17 Jun 1996 09:17:10 -0400
Subject: Re: Vibration isolator

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Go to the web site listed at the bottom of this messsage and you
will find our home page. Click on the " Tips & Tricks Wizard " and follow
the link to " All the other stuff " You will find a recent discussion on
vibration isolation which we archived off of this list recently. For any who
do not have web access, let me know and I will get you the information.





At 08:31 AM 6/14/96 -0400, you wrote:
}
} I had purchased an air-suspension type vibration isolation table
} (brand name withheld intentionally) for my research microscope.
} Even though the sales person explained that this was exactly what I
} wanted, it did not meet my needs. Once the system is suspended, one
} cannot touch the microscope, or else it takes considerable time for it to
} stop rocking back and forth. Although we could purchase a remote shutter
} release to solve part of the problem, our real problem is that *any*
} adjustment (stage movement, focus) causes considerable motion in the
} scope. We have had to use the table with the suspension system
} disengaged. The table is useful in that its frame is made of a
} heavy steel construction and the work surface is a 2-inch thick composite
} slab, which takes two people to lift. The heavy weight of the unit does
} help reduce vibration, but at a cist if $4,000 I bet we could have spent our
} money in better ways.
}
} If someone out there has recommendations, I, too, would like to hear them.
}
} ______________________________________________________________________
} Donald L. Lovett e-mail: lovett-at-trenton.edu
} Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} Trenton State College, NJ 08650-4700 fax: (609) 771-2674
}
}
}
}




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/





From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Mon, 17 Jun 1996 08:36:56 -0500
Subject: Re: Seeking advice on avoiding major pitfalls in elemental

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At 05:10 PM 6/14/96 -0500, you wrote:

}
} In response to the message:
} ======================================
} If anyone has experience in elemental microanalysis on thin sections of
} BIOLOGICAL material, we would very much appreciate a piece of advice, to
} avoid the major first-try mistakes.
} ==========================================================
} I wanted to mention that our best results ever obtained in our own lab
} and using by coincidence, a JEOL 100CX TEMSCAN as well, was done by
} picking up the sections on a silicon monoxide/dioxide filmed grid, and
} then exposing the now supported section for a few moments (actually
} about 20 seconds) the an oxygen plasma, which got rid of all the organic
} material, leaving the inorganics spread around in a what that leaves a
} ghost image of where the cell outlines were originally. But the big
} advantage is that once you have removed the organics this way, the
} Bremstrahlung radiation drops to nearly zero, thereby increasing your
} signal to noise ratio enormously.
}
******************
Chuck -

OK, this was done on the Plasma Prep II? That is an interesting technique;
and would be especially helpful if one were trying to find/demonstrate/rule
out trace elements which would be obliterated by background from organics.
However generally one wishes to show the location of the mineral in relation
to the tissue, in which case the tissue is best left behind. I'll try to get
by the SPI booth at the MSA meetings in August to take a look at that machine.

Joiner








From: David Garrett :      DGARRETT-at-gab.unt.edu
Date: Mon, 17 Jun 1996 15:22:14 CST6CDT
Subject: x-ray hardware TN5500

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I am trying to setup a Tektronix 4696 ink jet printer on a TN5500 and
need the switch settings on the back of the printer.
Thanks,
***************************************
David Garrett "DGARRETT-at-GAB.UNT.EDU"
University of North Texas
Dept. Biological Sciences
(817)565-3964 Fax (817)565-4136
***************************************




From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 6/12/96 11:29 PM
Subject: Core facility- to be or not to be.

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
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Conversion: allowed
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Priority: normal
Sensitivity: Company-Confidential


I know this issue has been discussed before and I would be very
appreciative of someone sharing the key ideas with me. We are able
to get money to upgrade our microscopy facility (confocal, EM and LM)
which currently is used exclusively by one dept., if we offer it
as a core facility to all the research groups in this pharmaceutical
research facility. Right now there is one staff person (me) to do EM
and I have my own research projects for the group. What possibility
is there of doing my own research project at the same time as providing
service for others. What is the minimum staff? How does one begin to
judge potential usage? Thanks for your thoughts and my apologies for
addressing issues that may have already been covered.
Pat Masarachia
Merck Research Laboratories
West Point, Pa 19486
phone 215-652-7999
e-mail pat_masarachia-at-merck.com







From: Michael T.K. Koh :      mkoh-at-ecn.purdue.edu
Date: Mon, 17 Jun 1996 09:30:22 -0500 (EST)
Subject: SiGe standard for EDS

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Hello,

I am looking for a standard to re-calibrate the EDS on our TEM specifically
for SiGe compositions. Appreciate for any help on this.

thanks,
Michael Koh
mkoh-at-ecn.purdue.edu




From: Elinor Solit :      cambrex-at-world.std.com
Date: Mon, 17 Jun 1996 19:01:48 -0400 (EDT)
Subject: Re: Oxford U.S. telephone

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Delilah

Oxford has an office in Concord, Massachusetts. You can reach them at
800-769-3673.

Regards,
Elinor Solit
The Microscope Book

On Mon, 17 Jun 1996, Delilah W. Irving wrote:

} Does anyone happen to have the address and phone number for Oxford
} Instruments, Ltd...they make cryo stages.
}
} Thank you
}
} Delilah W. Irving tel: 510-559-5653
} USDA - ARS - WRRC fax: 510-559-5777
} 800 Buchanan St. email: dirving-at-pw.usda.gov
} Albany, CA 94710
}
}





From: brannign-at-asrr.arsusda.gov (Peggy Brannigan)
Date: Mon, 17 Jun 1996 18:33:58 -0400
Subject: TEM: need antibody source

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Hello all,

Does anyone know of a commercial source of monoclonal antibodies
to resident proteins of plant ER, Golgi stacks, plasma membranes, and
vacuoles? Any of the vesicle transport proteins would be especially
wonderful! I've been able to find some for animal and yeast proteins but
no luck with plant proteins.

This would be for immunolabeling for TEM. I'm trying to determine
the origin of some unusual membranes and vesicles that are associated with
a viral infection. I'm also trying to find out if some Golgi-derived
membranes ( sites of virus budding) come from the trans or cis Golgi.

I'm very grateful for any help you can give me!

Peggy

Peggy Brannigan
Electron Microscopy
Floral and Nursery Plants Research Unit
National Arboretum

Bldg. 010A R.238
10300 Baltimore Avenue
Beltsville, MD USA20705

Phone: (301) 504-6097
Fax : (301) 504-5096
Email: brannign-at-asrr.arsusda. gov







From: rich-at-egr.msu.edu (Michael Rich)
Date: Mon, 17 Jun 1996 10:21:22 -0400
Subject: job opening

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A research associate is sought to establish, operate, and maintain the
Environmental Scanning Electron Microscope which is to be set up as a
University user and outreach facility. The individual is expected to conduct
research on materials characterization and behavior with the ESEM in
collaboration with faculty, students and government personnel. Research
areas include composite materials, metals, ceramics, polymers, fiber
reinforced cement, asphalts, food, packaging materials, soils, plants and
wood products. The ESEM is equipped with various stages for in situ
stress-temperature-environment studies over a wide range of conditions. The
ESEM is also equipped with an EDS detector for chemical analysis.

The person occupying this position will be responsible for operation and
maintenance of the ESEM as well as the training of faculty, staff, graduate
students and non-campus users. In addition, the successful candidate will
conduct research with the ESEM and is expected to develop contacts with
potential off-campus users in the local and state-wide community to assist
in generating funds for the support and maintenance of the ESEM. The
responsibilities for this position are divided approximately as follows:
Research-50%, Outreach-25%, Administration-15%, Teaching-10%.

Qualifications: A Ph.D. in Materials Science, Physics, Chemistry, Geology,
Biology or Engineering is required. A combination of course work and
hands-on research experience with SEM is required, ESEM is preferred. The
applicant must exhibit very high levels of oral and written communication
skills. Previous experience in budgeting and accounting would be useful.

Position is open until filled. Salary is commensurate with qualifications
and experience. Send a complete curriculum vitae, graduate level transcript,
and three references to:
Professor Lawrence T. Drzal,
Michigan State University, Composite Materials and Structures Center,
Engineering Research Complex B-100,
East Lansing, MI 48824-1326 tel: 517/ 353-5466 fax: 517/ 432-1634

Contact Michael Rich, Research Specialist and Laboratory Manager, for
further information regarding this position. RICH-at-EGR.MSU.EDU. Michigan
State University is an equal-opportunity institution.





Michael J. Rich
Specialist
Composite Materials and Structures Center
Research Complex-Engineering Rm C115
Michigan State University
East Lansing MI 48824-1326

tel: 517/353-4696
fax: 517/432-1634
e-mail: rich-at-egr.msu.edu




From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 6/17/96 4:08 PM
Subject: Oxford U.S. telephone

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Does anyone happen to have the address and phone number for Oxford
Instruments, Ltd...they make cryo stages.

Thank you

Delilah W. Irving tel: 510-559-5653
USDA - ARS - WRRC fax: 510-559-5777
800 Buchanan St. email: dirving-at-pw.usda.gov
Albany, CA 94710







From: Charles A. Garber :      103532.3325-at-CompuServe.COM
Date: 18 Jun 96 00:10:56 EDT
Subject: Re: Seeking advice on avoiding major pit

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In response to my message to Joiner Cartright, and which he re-posted in part to
the listserver, I had originally said:
=======================================================
...... that our best results (we) ever obtained (were done) in our own lab and
using by coincidence, a JEOL 100CX TEMSCAN as well, was done by picking up the
sections on a silicon monoxide/dioxide filmed grid, and then exposing the now
supported section for a few moments (actually about 20 seconds) in an oxygen
plasma, which got rid of all the organic material, leaving the inorganics spread
around (in a way) that leaves a ghost image of where the cell outlines were
originally. But the big advantage is that once you have removed the organics
this way, the Bremstrahlung radiation drops to nearly zero, thereby increasing
your signal to noise ratio enormously.
=============================================================
Joiner then replied, in part:

However generally one wishes to show the location of the mineral in
relation to the tissue, in which case the tissue is best left behind.
=============================================================
The point is that the inorganics and perhaps unashed material of unknown
composition tend to be scattered in situ on the silicon monoxide/ dioxide
support film (itself which won't be etched with an oxygen plasma) which creates
the apparance of cell "ghosts", that is, the outlines of where the cell walls
used to be, in otherwords, one does not really lose completely the orientation
of the inorganics relative to the cell outlines and other structures present.
This might not be true for all samples, but it has been true for samples we
ourselves have etched, and I can also recall on at least two different
occasions, other users of the technique coming by our exhibit booths over the
years (sorry don't remember names) showing similar micrographs. The "keys to
success" are a high quality silicon monoxide/dioxide support film and the
ability to do a very gentle (e.g. room temperature, low power, short etch time)
etching time with pure oxygen. Yes, the SPI Plasma Prep II is one of several
bench top units produced that have been used for this purpose, however, in a
different place I could agrue that the SPI unit is more optimum for this
particular application (e.g. the "manifold" design of the chamber).

I will bring at least one good example of this applicaton of plasma etching to
MSA. Also, if anyone is interested, I will see about the quality of a photocopy
and send it by snail mail for anyone asking for a copy.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.com

Look us up!
###########################
WWW: http://www.2spi.com
###########################
======================================================





From: ebs-at-ebsciences.com
Date: Tue, 18 Jun 1996 06:20:38 -0500
Subject: Re: Antigen Retrieval

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Gary Login wrote:
{My understanding of Dr. Shi's take home message from a recent workshop was
that most antigens can be retrieved from formaldehyde tissue heated between
95-100 degrees C- for 20-30 minutes- at pH 1-2 (the buffer or salt solution
is not important). In fact acid or base pH is superior to neutral pH for
most tissue sections.}

The easiest way to do this is with a laboratory microwave with an accurate
temperature probe. The probe is inserted into the antigen retrieval
solution, the time and temperature is set, and that's that. We have
reprints available of some of Dr. Shi's articles in Cell Vision magazine
documenting this technique. Please contact me back-channel for copies.

Disclaimer: Energy Beam Sciences, Inc. manufactures the laboratory
microwave used in some of Dr. Shi's published studies.

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Tue, 18 Jun 1996 09:14:37 -0400
Subject: CM-12 or similar wanted.

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Hi there, I am looking to upgrade my microscope that I use for basic
research and teaching. I would like to get hold of a CM-12 or similar and
it would be a bonus if it has an XEDS system. I do not want a 420, 400 or
older! I currently have a 420.
Please respond by email or phone.

Thanks.

John Mansfield.


John Mansfield
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html






From: monroe-at-emcenter.msstate.edu (William A. Monroe)
Date: Tue, 18 Jun 1996 08:51:20 -0500
Subject: TEM: Flagella Processing Procedures

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To the List: I am looking for a conventional EM, chemical fixation
protocol for processing flagella from Trichomonas Vaginalis. The objective
of the work is the preservation of the 9+2 microtubule arrangement in the
flagella.

Thank You,

Bill Monroe






From: tania-at-dynamotive.com (Tania Jones)
Date: Tue, 18 Jun 1996 08:17:45 -0700
Subject: EDX: TN 5500

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Help!

Our TN 5500 will not boot. Turning on the power only causes the fan to
turn on; the drives won't even kick in. Any suggestions as to what the
problem is and if it can be fixed?

Thanks in advance.

Tania Jones
Laboratory Manager
DynaMotive Technologies

phone:(604)222-5521





From: garyc-at-stud.unit.no (Gary)
Date: Tue, 18 Jun 1996 21:35:53 +0200 (MET DST)
Subject: Reference marks

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Hi!

Im wondering if somebody outthere knows a simple way of making reference
marks in the block face. The marks must have a diameter of max. 5-15
micrometer. The reference marks are going to be used in order to register
(align) the serial sections, and finally make a 3D reconstruction of a
plant cell.

Please when you reply this message send it to this email adress:

gary-at-nvg.unit.no

in advance, thanks...


/////////////////////////////////////////////////////////////////
// // //

// Gary Chinga // email :garyc-at-james.avh.unit.no //
// Plantebiosenteret // WWW :http://www.nvg.unit.no/~gary //
// NTNU, 7055 Dragvoll // phone : 73590168 //
// Norway // fax : 73590177 //
// // //
/////////////////////////////////////////////////////////////////






From: Marc C. Brande, MS, Founder (619) 587-4830 FAX: (619) 552-1516 :      mcbrande-at-sierra.net
Date: Tue, 18 Jun 1996 15:19:38 +0000
Subject: LiveCell Timelapse Capability in San Diego/L.A.?

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Message-Id: {31C6C90A.4205-at-sierra.net}
confocal-at-ubvm.cc.buffalo.edu, microscopy-at-Sparc5.Microscopy.Com,
spm-at-di.com, schibler-at-ljcrf.edu, sivbiology-at-aol.com

Are there facilities (academic or commercial) in San Diego/Los Angeles
area that offer live-cell timelapse imaging service for a fee?

Thanks in advance for any leads.

Marc




From: Eric Kokko :      kokko-at-em.agr.ca
Date: Tue, 18 Jun 1996 16:02:01 -0400
Subject: >>>>>>>>>>>>>>>

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Message-Id: {s1c6e6c3.088-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

} } } } } } } } } } } } } } }
Help!

Our TN 5500 will not boot. Turning on the power only causes the fan
to turn on; the drives won't even kick in. Any suggestions as to what
the problem is and if it can be fixed?

Thanks in advance.

Tania Jones
Laboratory Manager
DynaMotive Technologies

phone:(604)222-5521


{ { { { { { { { { { { { { { {

Tania,

We have the same problem with our TN5500. As far as I can tell,
either the POWER SUPPLY or the DISK DRIVE has packed it in. I
haven't had time to check this out yet (...and I can't afford a
service engineer to come at this time).

If you get any diagnostics from elsewhere, please broadcast the info,
as there are a lot of TN5500's out there.

Cheers!

Eric Kokko Phone: 403-327-4591 (Ext.367)
Agriculture Canada EMail: kokko-at-em.agr.ca
Lethbridge Research Center
Lethbridge, Alberta
CANADA





From: tsi-at-werple.net.au (Thomson Scientific:Paul Thomson)
Date: Wed, 19 Jun 1996 15:57:54 +1000 (EST)
Subject: >>>>>>>>>>>>>>>

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} } } } } } } } } } } } } } }
Help!

Our TN 5500 will not boot. Turning on the power only causes the fan
to turn on; the drives won't even kick in. Any suggestions as to what
the problem is and if it can be fixed?

Thanks in advance.

Tania Jones
Laboratory Manager
DynaMotive Technologies

phone:(604)222-5521


{ { { { { { { { { { { { { { {

Tania,

We have the same problem with our TN5500. As far as I can tell,
either the POWER SUPPLY or the DISK DRIVE has packed it in. I
haven't had time to check this out yet (...and I can't afford a
service engineer to come at this time).

If you get any diagnostics from elsewhere, please broadcast the info,
as there are a lot of TN5500's out there.

Cheers!

Yes that is the most likely problem. You might want to try a manual boot
as per manual instructions!!!!
Can you hear the hard drive spinning? A common occurance is that the hard
drive will wind up and them wind down and so on. The hard drive is
definately gone.
The power supply - there are two main power supplies on the TN5500, one
controlling the floppies/hard drive and the other the rest.
Check to see if there is a +5 and +12 supply to the hard/floppy drives. If
not, a
quick(well maybe not so quick?) or cheap solution would be to get the supply
from somewhere else, ie the main supply. There is a +5 and 12V supply there.
Simply disconnect the existing power from the hard drive and re-route to the
main supply. Note : the main power supply is difficult to get to( it is on
the left hand side as you look at the TN5500 from the back) . You have to
take out the main unit.

Henry Kudric
Thomson Scientific Instruments
Melbourne, Australia





From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Wed, 19 Jun 1996 18:12:42
Subject: Re: Baking contaminated apertures

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To: microscopy-at-Sparc5.Microscopy.Com

Im having trouble baking contamination off molybdenum strip apertures. Quite
a lot comes off with a 15 minute bake in a moly tray at red heat but there is
a resistant residue which this moderate baking wont shift. Should I give up
and let chuck sell me new ones, or heat them more, or longer, or is there some
other cleaning system I don't know about?

mel dickson
UNSW Sydney, land of Oz




From: chris gilpin :      cgilpin-at-fs1.sem.man.ac.uk
Date: Wed, 19 Jun 1996 09:35:56 BST
Subject: Re: EM field problem

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} From: GIGNAC-at-watson.ibm.com
} Date: Tue, 18 Jun 96 09:53:21 EDT
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: EM field problem

} We have just installed a JEOL 840-F FE-SEM in a lab at IBM-Watson Research
} Center, and the room has large EM fields that hinder low KeV operation. We
} might try shielding the microscope but have been told that shielding has not
} been very successful in the past. I was told that there now exists equipment
} that can sense and compensate for EM fields. Does anyone know who are the
} manufacturers of this type of equipment and if so how well the equipment works?
} Please respond to: gignac-at-watson.ibm.com or call 914-945-3352.


Oxford instruments in the UK sell a field cancelling system. We have
recently fitted one to a JEOL 1200 and it works very well. Cost in
the UK is about stlg7K.

Chris

Chris Gilpin
Biological Sciences Electron Microscope Unit
G452 Stopford Building
Oxford Road
Manchester
M13 9PT
phone +44 161 275 5170
fax +44 161 275 5171




From: KREHMEYER-at-odin.ssec.honeywell.com
Date: Wed, 19 Jun 1996 11:32:58 -0500 (CDT)
Subject: subscribe

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Message-Id: {199606191319.JAA21515-at-thomas.ge.com}

subscribe




From: KREHMEYER-at-odin.ssec.honeywell.com
Date: Wed, 19 Jun 1996 11:32:58 -0500 (CDT)
Subject: subscribe

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subscribe




From: paulc-at-gps.caltech.edu (Paul K. Carpenter)
Date: Wed, 19 Jun 1996 09:47:30 -0700
Subject: Re: TN 5500 Problems

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} Help!
}
} Our TN 5500 will not boot. Turning on the power only causes the fan to
} turn on; the drives won't even kick in. Any suggestions as to what the
} problem is and if it can be fixed?
}
} Thanks in advance.

Doug Conners runs TN Analyzer Service, and has been quite helpful in
keeping our aging TN 5500 systems running. He can be reached at
608-798-2005 or FAX 608-798-1675.

My guess is that at the very least he could tell you what the problem is
and offer you the hardware solution.

Good luck,

Paul Carpenter


+----------------------------------------------------+
| Paul K. Carpenter paulc-at-gps.caltech.edu |
| Division Analytical Facility |
| Geological and Planetary Sciences MC 170-25 |
| California Institute of Technology |
| Pasadena, CA 91125 |
| 818-395-6126 (X-ray Lab) 818-568-0935 (Dept. FAX) |
+----------------------------------------------------+






From: Joe Fu :      jofu-at-enh.nist.gov
Date: Wed, 19 Jun 1996 14:34:11 -0400
Subject: Re: Baking contaminated apertures

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Message-Id: {2.2.32.19960619183411.006ed878-at-mailserver.nist.gov}
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At 06:12 PM 6/19/96, Mel Dickson wrote:
} Im having trouble baking contamination off molybdenum strip apertures. Quite
} a lot comes off with a 15 minute bake in a moly tray at red heat but there is
} a resistant residue which this moderate baking wont shift. Should I give up
} and let chuck sell me new ones, or heat them more, or longer, or is there some
} other cleaning system I don't know about?
}
}
}
Hi, Mel:

Untrasonicating in acetone for 10 min. followed by another 10 min. in
alcohol before baking in vacuum may help to remove these residues. Good luck.


Joseph Fu
National Institute of Standards and Technology
Room A117, Building 220
Gaithersburg, Maryland 20899-0001

Tel:301-975-3495
e mail: jofu-at-nist.gov
Fax: 301-869-0822





From: Mike Witcomb :      MIKEW-at-gecko.biol.wits.ac.za
Date: Wed, 19 Jun 1996 18:56:43 GMT+2
Subject: Ultramicroscopy is alive and well

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L.D.Marks asked if Ultramicroscopy was dead since the last issue he
had seen was about November 1995.

I asked Elsevier, the publishers, and their reply is given below. So
keep sending articles, we still need them. All Elmar's work is not
lost!

"We have been gradually changing to an alternative(electronic)
publication process and when it came to Ultramicroscopy's turn somehow the
transition did not go smoothly at all; everything went wrong that could go
wrong.

My records show that the last issue was Vol 60, No. 4 (the Master Index). I
have just been informed that Volume 61/1-4 and 62/1-2, 62/3 and 62/4 were
published yesterday. They should be despatched today or tomorrow. This still
leaves us a little behind on the planned schedule, however.

So the answer to the question is that the journal is most certainly not dead,
but production problems at the publishers have caused a delay in the schedule.
Everyone here is working hard to bring the journal back on schedule."




Dr MJ Witcomb
Electron Microscope Unit
University of the Witwatersrand
Private Bag 3
WITS
2050
South Africa

Telephone: + 27 11 716 4000
+ 27 11 716 2419 (messages)
Fax: + 27 11 339 3407
E-mail: mikew-at-gecko.biol.wits.ac.za




From: bozzola-at-siu.edu (John J. Bozzola)
Date: Wed, 19 Jun 1996 14:27:20 -0600
Subject: CSMS Meeting Program (long)

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The program and directions to the meeting site for the CSMS-MIKMAS (Central
States Microscopy Society and Missouri-Illinois-Kansas Microbeam Analysis
Society) meeting on June 28 are enclosed. Contact me if you have further
questions

CSMS--MIKMAS MEETING PROGRAM
June 28, 1996 at The Lodge, Giant City State Park

8:30 - 9:00 AM REGISTRATION: Membership renewals, lunch tickets ($8.50).
See Lou Ann Miller or Mike Veith to obtain tickets for lunch at the
main table.

9:00 - 9:20 **Nadia E. Navarrete-Tindall, Dept Plant Biology, SIUC
Nodule and Rhizobial Morphology of the Tropical Legume Gliricidia sepium

9:20 - 9:40 **Barrie Overton, Plant Biology, SIUC
Fixation Techniques for Morphological Investigations in the
Mucoraceae, zygomycetes

9:40 - 10:00 **Elden Neal, Plant Biology, SIUC
Flatbed Scanners for Scientific Imaging of Plant Material

10:00 - 10:20 **Rita K. Ware, W. Sherman, J. Craig, J. Schmelz, Dept
Chem/Physics and Biological Sciences, Chicago State University. Electron
Microscopy of Halobacterium halobium
and cell membrane regions containing bacteriodopsin

10:20 - 10:40 COFFEE BREAK: COURTESY OF ELECTRON MICROSCOPY SCIENCES
Membership renewals, lunch tickets ($8.50).See Lou Ann Miller or
Mike Veith
to obtain tickets for lunch at the main CSMS-MIKMAS table.

10:40 - 11:00 **Mohan Kalyanaraman, Dept Metallurgy, U of Connecticut,
Storrs, CT
In situ Electron Microscopy of WC-Co Nanostructured Composites

11:00 - 11:20 **Marlies Webber, Dept Plant Biology, SIUC
The Role of Actin in Spermatogenesis of the Liverwort Conocephalum

11:20 - 11:50 Dr. Karen Renzaglia, Dept Plant Biology, SIUC
Ultrastructure and the Phylogeny of Land Plants

12:00-12:30 Dr. Aristotel J. Pappelis and Sidney W. Fox , Plant
Biology, SIUC
Domain Protolife: Thermal Protein -- First Paradigm

12:30-1:30 LUNCH in the Lodge

1:30-2:30 Steven Slap, Energy Beam Sciences, Inc., Agawam, MA
Microwave Techniques for Microscopy

2:30-2:50 Mike Veith, Biology Dept, Washington U, St. Louis, MO
Use of Microwave Oven for Immunogold Localization in Transgenic
Alfalfa Leaves

3:00 ROUNDTABLE DISCUSSIONS AND SCIENTIFIC DEMONSTRATIONS

Lou Ann Miller, Dept Veterinary Medicine, U of I, Champaign, IL
Roundtable Discussion/Demonstration of Microwave Experiences
NOTE: bring your micrographs, prints, protocols and handouts for
distribution

John Gnaedinger, Hitschfel Instruments, Inc.
Demonstration of Proper Techniques for the Care, Cleaning and
Alignment of
Light Microscopes

BUSINESS MEETING

**Denotes Student Competition Presentation

---------------------------------------------

HOTELS, MOTELS:

Comfort Inn Best Inns of America Super 8
Motel
1415 E. Main 1345 E. Main 1180 E.
Main
618-549-4244 529-4801 457-8822
$40-45.00 $34.00 $47-49.00

DIRECTIONS:

IF COMING INTO CARBONDALE FROM MARION (off of route 57 and onto route 13
West), the first main stop lights that you encounter just outside of
Carbondale will be at Point A on the map (MacDonald's is on your right).
Super 8 Motel and Holiday Inn would be further down route 13 and on your
right. Turning left at Point A at the light, followed by a right turn at
your next opportunity will get you on the frontage road to the Best Inns of
America (near the large Mall). Turning left would get you to the Comfort
Inn (near Pier 1 store). Turning left at A (but continuing straight) will
also get you onto the Giant City Road to the meeting site. In this case,
after going through one stop sign, continue straight ahead on the Giant
City Road until it ends (about 11 miles). You will pass LIttle Grassy Road
and the SIU Touch of Nature compound on your left. Continue straight on the
road until it ends at the intersection marked "B". Turn left and follow the
road until you see the parking lot for the main lodge. This is about 3/4
miles and just past some cabins on your right. Pull into the main lot with
the large water tower, parking as close as possible to the lodge building.
Come into the lodge.

IF COMING FROM MURPHSBORO, take Route 13 E through Carbondale (towards
Marion). It is easiest to pass by the Mall on your right and to turn right
at Point A, as directed in the paragraph above.

IF COMING TO CARBONDALE ON ROUTE 51 FROM THE NORTH, take Route 13 East
towards Marion and Route 57 and pass by the Mall on your right and turn
right at Point A, as directed in the first paragraph above.

IF COMING TO CARBONDALE ON ROUTE 51 FROM THE SOUTH, take the turn off to
Makanda on your right (near the large water tower with a smiling face
painted on it). You will pass down some steep, winding hills and eventually
into Makanda. After passing the RR track, turn left, proceed about 1/2 mile
until you see the sign to the Park. Turn right and enter the park. Follow
the signs to the lodge.

EMERGENCY TELEPHONE NUMBERS: Giant City Lodge (618-457-4921), John
Bozzola home phone (618-529-5099).

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++








#############################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
#############################################################################






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 19 Jun 1996 17:29:26 -0400
Subject: Mel/Cleaning Mo Apertures

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Message-ID: {n1376917026.4763-at-mse.engin.umich.edu}

Subject: Time: 5:23 PM
OFFICE MEMO Mel/Cleaning Mo Apertures Date: 6/19/96

Mel Dickinson:

I am afraid that if you have already heated the apertures to a red heat, the
residue (or whatever it is you see on it) is probably no longer organic in
character, and so will probably not be affected by any organic solvent.

The simplest approach would be to try the apertures with the "residue" on
them. It is just possible that whatever you are seeing is actually a surface
effect, and will not affect the character of the hole itself.

Next, you might try an ultrasonic treatment in a strong solution of Tilex
Soap Scum Remover. This is a pretty powerful cleaning agent, and might help
if the residue is loosely deposited on the surface.

Then, you might escalate the attack to using an etchant for Mo (the standard
reagent for etching Mo being Murikami's reagent: 10 gm potassium ferrocyanide
and 10 gm KOH or 7 gm NaOH dissolved in 100 ml water), or a chemical
polishing reagent (such as 30 ml lactic acid, 10 ml nitric acid and 5 ml
hydrofluoric acid).

When all else fails, ask Chuck Garber; he usually knows a lot about such
matters.






From: Joe Fu :      jofu-at-enh.nist.gov
Date: Wed, 19 Jun 1996 14:34:11 -0400
Subject: Re: Baking contaminated apertures

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Message-ID: {n1376917354.86038-at-mse.engin.umich.edu}
"microscopy-at-Sparc5.Microscopy.Co" {microscopy-at-Sparc5.Microscopy.Com}
X-Mailer: Mail*Link SMTP/QM 3.0.0GM

Reply to: RE} } Baking contaminated apertures

I am afraid that if you have already heated the apertures to a red heat, the
residue (or whatever it is you see on it) is probably no longer organic in
character, and so will probably not be affected by any organic solvent.

The simplest approach would be to try the apertures with the "residue" on
them. It is just possible that whatever you are seeing is actually a surface
effect, and will not affect the character of the hole itself.

Next, you might try an ultrasonic treatment in a strong solution of Tilex
Soap Scum Remover. This is a pretty powerful cleaning agent, and might help
if the residue is loosely deposited on the surface.

Then, you might escalate the attack to using an etchant for Mo (the standard
reagent for etching Mo being Murikami's reagent: 10 gm potassium ferrocyanide
and 10 gm KOH or 7 gm NaOH dissolved in 100 ml water), or a chemical
polishing reagent (such as 30 ml lactic acid, 10 ml nitric acid and 5 ml
hydrofluoric acid).

When all else fails, ask Chuck Garber; he usually knows a lot about such
matters.




As a simple approach you might try an ultrasonic treatment in a strong
solution of Tilex Soap Scum Remover. This is a pretty powerful cleaning
agent, and might help.

Otherwise


--------------------------------------

} and let chuck sell me new ones, or heat them more, or longer, or is there
some
} other cleaning system I don't know about?
}
}
}
Hi, Mel:

Untrasonicating in acetone for 10 min. followed by another 10 min. in
alcohol before baking in vacuum may help to remove these residues. Good
luck.


Joseph Fu
National Institute of Standards and Technology
Room A117, Building 220
Gaithersburg, Maryland 20899-0001

Tel:301-975-3495
e mail: jofu-at-nist.gov
Fax: 301-869-0822


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From: hua-at-junction.ucsb.edu (Wu Xuehua)
Date: Wed, 19 Jun 1996 16:15:57 -0800
Subject: unsubscribe

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Message-Id: {199606192314.QAA27034-at-surface.ucsb.edu}

unsubscribe, please
Xuehua Wu
Materials Department
University of california, Santa Barbara
CA 93106
Tel: 805 893 8523





From: Lou Ann Miller :      lmiller-at-ux1.cso.uiuc.edu
Date: Wed, 19 Jun 1996 18:59:42 -0500
Subject: Central States: Program and Directions

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Message-ID: {31C8946E.61B3-at-ux1.cso.uiuc.edu}

Greetings all!

Because the WWW page for Central States is new, I'm dropping a line to let everyone know that

for the June 28th Microscopy Meeting:


1. You can look up the Program Schedule and Hotels Listings at:

http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/CSMS/Spring96Prog



2. Directions and a map are found at:

http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/CSMS/maps

*** If anyone can't get to these pages, email me , and I will send you a copy electronically.

Thanks,
Lou Ann Miller -- Secretary-- CSMS




From: Probing & Structure :      pns-at-ultra.net.au
Date: Thu, 20 Jun 1996 10:58:46 +1000
Subject: Re: Baking contaminated apertures

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you wrote:
Im having trouble baking contamination off molybdenum strip apertures. Quite
a lot comes off with a 15 minute bake in a moly tray at red heat but there is
a resistant residue which this moderate baking wont shift. Should I give up
and let chuck sell me new ones, or heat them more, or longer, or is there some
other cleaning system I don't know about?

mel dickson
UNSW Sydney, land of Oz
***************************

Chances are that these apertures are goners. When cleaning either Pt, Mo or
W apertures, it is important to place them for a while, in a strong solvent
(chloroform) and perhaps to sonnicate them. This would at least eliminate
any soluble and loose residues. Then Mo and W apertures are heated in vacuo
on a Mo or W strip or boat. The most common mistake is over-heating, which
causes a coarse crystal structure and makes apertures useless. Heating to a
dull, dark cherry red only is recommended but the heating time can be
lengthy, say five minutes. Unfortunately, some contaminants become quite
unmovable. Various physical methods, like polishing both sides of an
aperture on a slide or poking it with a glass-fibre, have been tried, but
usually it is a waste of time; chucking them is better.

Jim Darley



Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
Internet Catalogue: http://www.ultra.net/~pns/





From: EvexAnalyt-at-aol.com
Date: Wed, 19 Jun 1996 23:33:13 -0400
Subject: Re: EDX: TN 5500

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Evex Analytical Instruments
Princeton, New Jersey

(908)874-3800
EvexAnalyt-at-aol.com
www.evex.com

Enclosed is information about Evex Analytical Instruments Service. Evex is
the world's leading independent provider of service and support for Evex,
Link, Kevex, Noran, P.G.T. and Tracor brand EDS systems and related
peripherals. Our Service Engineers are “factory trained” and are located
nationwide.

EDS Systems

New -- WinEDS High Performance X-Ray Microanalysis System
Service -- Evex, Link, Kevex, Noran, PGT, Tracor
Detectors -- New, Service, Upgrades
Pulse Processor -- VIDX Univeral Pulse Processor

VIDX Digital Imaging
VIDX "Interfazer" -- Transfer Spectra & Images at Lighting Speed to a PC
VIDX Scan CCD -- Cooled, Scientific grade, 1536 x 1024, 14 bit, PC parallel
port interface
VIDX Scan SEM -- Digitize your SEM Image
VIDX Scan IPS -- Image Processing Software (User Definable File Formats)
Image Pro -- Worlds Greatest Image Processing Software

Lab Networking & Video Conferencing
VIDX Vision -- Researchers show others exactly what you are talking about

The accuracy of your data can be greatly reduced if your equipment has not
been thoroughly cleaned, calibrated and tested.

Ask your self

- Is your system in calibration?
- Are your power supplies at acceptable levels?
- Has your EDS detector ever been cleaned
- Has your microscope mag been calibrated lately? or discriminators
adjusted?
- Have you ever taken a good look at the PHA page?
- Have you ever changed your fan filters?
- Do your disk drives operate properly?
- Have users been fully trained running the programs?

If you answered no to any of the above, then you should consider either our
Managed Care Service Agreement (MCSA) or our On-Demand Service. For your
information, below is our present rate schedule.

Rates
- On-site service $100.00 portal to portal (2hr min.)
- Installation $100.00 portal to portal (2hr min.)
- Managed Care Service Agreement (call for a quote)
- Training on-site $1,000.00 per/day

Parts, airfare, lodging, mileage, rental car and miscellaneous
charges.(Extra)

The combination of Multi Vendor Service and our unparalleled commitment to
quality makes Evex Analytical Instruments the vendor of choice . If you have
any questions about our services or have a specific requirement not listed
above, please call me at (908) 874-3800. Evex Analytical Instruments is
willing to work closely with you to develop specific programs customized to
meet your needs.




From: Jouko =?iso-8859-1?Q?M=E4ki?= :      jouko.maki-at-utu.fi
Date: Thu, 20 Jun 1996 08:23:42 +0300
Subject: www-homepage

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To: microscopy-at-Sparc5.Microscopy.Com

Hello all,

I am perhaps misusing this forum by introducing to you the existence of our
www-homepage at:
http://www.utu.fi/tdk/laak/em/index.html

Best wishes for a nice summer from wet and cool Finland

Jouko

Jouko K. M=E4ki, Ph.D., Laboratory Manager
University of Turku, Laboratory of Electron Microscopy
Kiinamyllynkatu 10 FIN-20520 TURKU FINLAND
Tel: +358 21 333 7318 GSM: +358 40 505 2521 FAX: +358 21 333 7380





From: shaun.sandow-at-anu.edu.au (Shaun Sandow)
Date: Thu, 20 Jun 1996 15:54:43 +1000
Subject: unicryl

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Dear All,

Has anyone using Unicryl resin for immunoEM (with low temperature embedding)
experienced it going viscous at -20oC after a final infiltration step of 1
: 3 (EtOH:resin)? ie. the tissue (bits of sciatic nerve) was dehydrated
and infiltrated as per the manufacturers instructions. The precooled resin
went viscous as soon as it was placed in the FS unit (before the tissue was
added) - the preparation is in the embedding medium now, but we were
wondering if the viscous resin was what we would expect to observe?

Thanks,

______________________________________________

Shaun Sandow
Autonomic Synapse Group
Division of Neuroscience
John Curtin School of Medical Research
Australian National University
ACT 0200

Ph. (06) 249 4782 (work) (06) 247 6430 (home)
Fax. (06) 249 2687
Email: shaun.sandow-at-anu.edu.au





From: lmaser-at-Sparc5.Microscopy.Com (Larry Maser)
Date: Thu, 20 Jun 1996 05:37:38 -0500
Subject: Re: CSMS Meeting Program (long)

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Dear John,

I'm not sure if Karen Horsley had coordinated with you before she left our
employ, sorry for the redundancy if she had. May we or have we sent a bunch
of brochures and other promotional materials for MSA and M&M '96 for
distribution at your local meeting? If we haven't and we may, how many
people shall we plan for? Also, any particular means of distribution is
fine, but it is even better for us if you can stuff materials into a package
which registrants will receive, or otherwise make them easily available and
well distributed.

I'll wait to hear from you,

Thanks,

Larry

At 02:27 PM 6/19/96 -0600, John J. Bozzola wrote:
} The program and directions to the meeting site for the CSMS-MIKMAS (Central
} States Microscopy Society and Missouri-Illinois-Kansas Microbeam Analysis
} Society) meeting on June 28 are enclosed. Contact me if you have further
} questions
}
} CSMS--MIKMAS MEETING PROGRAM
} June 28, 1996 at The Lodge, Giant City State Park
}
} 8:30 - 9:00 AM REGISTRATION: Membership renewals, lunch tickets ($8.50).
} See Lou Ann Miller or Mike Veith to obtain tickets for lunch at the
} main table.
}
} 9:00 - 9:20 **Nadia E. Navarrete-Tindall, Dept Plant Biology, SIUC
} Nodule and Rhizobial Morphology of the Tropical Legume Gliricidia
sepium
}
} 9:20 - 9:40 **Barrie Overton, Plant Biology, SIUC
} Fixation Techniques for Morphological Investigations in the
} Mucoraceae, zygomycetes
}
} 9:40 - 10:00 **Elden Neal, Plant Biology, SIUC
} Flatbed Scanners for Scientific Imaging of Plant Material
}
} 10:00 - 10:20 **Rita K. Ware, W. Sherman, J. Craig, J. Schmelz, Dept
} Chem/Physics and Biological Sciences, Chicago State University. Electron
} Microscopy of Halobacterium halobium
} and cell membrane regions containing bacteriodopsin
}
} 10:20 - 10:40 COFFEE BREAK: COURTESY OF ELECTRON MICROSCOPY SCIENCES
} Membership renewals, lunch tickets ($8.50).See Lou Ann Miller or
} Mike Veith
} to obtain tickets for lunch at the main CSMS-MIKMAS table.
}
} 10:40 - 11:00 **Mohan Kalyanaraman, Dept Metallurgy, U of Connecticut,
} Storrs, CT
} In situ Electron Microscopy of WC-Co Nanostructured Composites
}
} 11:00 - 11:20 **Marlies Webber, Dept Plant Biology, SIUC
} The Role of Actin in Spermatogenesis of the Liverwort Conocephalum
}
} 11:20 - 11:50 Dr. Karen Renzaglia, Dept Plant Biology, SIUC
} Ultrastructure and the Phylogeny of Land Plants
}
} 12:00-12:30 Dr. Aristotel J. Pappelis and Sidney W. Fox , Plant
} Biology, SIUC
} Domain Protolife: Thermal Protein -- First Paradigm
}
} 12:30-1:30 LUNCH in the Lodge
}
} 1:30-2:30 Steven Slap, Energy Beam Sciences, Inc., Agawam, MA
} Microwave Techniques for Microscopy
}
} 2:30-2:50 Mike Veith, Biology Dept, Washington U, St. Louis, MO
} Use of Microwave Oven for Immunogold Localization in Transgenic
} Alfalfa Leaves
}
} 3:00 ROUNDTABLE DISCUSSIONS AND SCIENTIFIC DEMONSTRATIONS
}
} Lou Ann Miller, Dept Veterinary Medicine, U of I, Champaign, IL
} Roundtable Discussion/Demonstration of Microwave Experiences
} NOTE: bring your micrographs, prints, protocols and handouts for
} distribution
}
} John Gnaedinger, Hitschfel Instruments, Inc.
} Demonstration of Proper Techniques for the Care, Cleaning and
} Alignment of
} Light Microscopes
}
} BUSINESS MEETING
}
} **Denotes Student Competition Presentation
}
} ---------------------------------------------
}
} HOTELS, MOTELS:
}
} Comfort Inn Best Inns of America Super 8
} Motel
} 1415 E. Main 1345 E. Main 1180 E.
} Main
} 618-549-4244 529-4801 457-8822
} $40-45.00 $34.00 $47-49.00
}
} DIRECTIONS:
}
} IF COMING INTO CARBONDALE FROM MARION (off of route 57 and onto route 13
} West), the first main stop lights that you encounter just outside of
} Carbondale will be at Point A on the map (MacDonald's is on your right).
} Super 8 Motel and Holiday Inn would be further down route 13 and on your
} right. Turning left at Point A at the light, followed by a right turn at
} your next opportunity will get you on the frontage road to the Best Inns of
} America (near the large Mall). Turning left would get you to the Comfort
} Inn (near Pier 1 store). Turning left at A (but continuing straight) will
} also get you onto the Giant City Road to the meeting site. In this case,
} after going through one stop sign, continue straight ahead on the Giant
} City Road until it ends (about 11 miles). You will pass LIttle Grassy Road
} and the SIU Touch of Nature compound on your left. Continue straight on the
} road until it ends at the intersection marked "B". Turn left and follow the
} road until you see the parking lot for the main lodge. This is about 3/4
} miles and just past some cabins on your right. Pull into the main lot with
} the large water tower, parking as close as possible to the lodge building.
} Come into the lodge.
}
} IF COMING FROM MURPHSBORO, take Route 13 E through Carbondale (towards
} Marion). It is easiest to pass by the Mall on your right and to turn right
} at Point A, as directed in the paragraph above.
}
} IF COMING TO CARBONDALE ON ROUTE 51 FROM THE NORTH, take Route 13 East
} towards Marion and Route 57 and pass by the Mall on your right and turn
} right at Point A, as directed in the first paragraph above.
}
} IF COMING TO CARBONDALE ON ROUTE 51 FROM THE SOUTH, take the turn off to
} Makanda on your right (near the large water tower with a smiling face
} painted on it). You will pass down some steep, winding hills and eventually
} into Makanda. After passing the RR track, turn left, proceed about 1/2 mile
} until you see the sign to the Park. Turn right and enter the park. Follow
} the signs to the lodge.
}
} EMERGENCY TELEPHONE NUMBERS: Giant City Lodge (618-457-4921), John
} Bozzola home phone (618-529-5099).
}
} ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
}
}
}
}
}
}
}
}
} #############################################################################
} John J. Bozzola, Ph.D., Director
} Center for Electron Microscopy
} Southern Illinois University
} Carbondale, IL 62901-4402
} U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} #############################################################################
}
}
}
}





From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Thu, 20 Jun 1996 08:20:33 -500
Subject: Confocal Listserver Address

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Can some one provide me with the listserver address for the confocal
group?

Thank you.

Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu




From: ekurz-at-mail.ims.uconn.edu (Ed Kurz)
Date: Thu, 20 Jun 1996 11:20:05 -0500
Subject: Bubble Analysis

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I have need to investigate bubbles which form between the surfaces of two
borosilicate glasses during a high temperature fusing operation. I would
like to be able to analyze any residual inorganics on the interior surface
of the bubble. We are able to fracture the sample and expose the bubble,
however due to the shape we have been unable to obtain adequate x-ray
signal from within the bubble.

We are also considering analyzing any gases contained in the bubble,
possibly by GC/MS techniques at elevated temperatures. We are concerned
that the material in the bubble may be the same as or similar to that on
the exterior surface.

Does anyone have experience in analyzing bubbles in glass or similar
materials? Suggestions?

Thanks in advance.

Ed Kurz
Institute of Materials Science
University of Connecticut
ekurz-at-mail.ims.uconn.edu







From: roy-at-bayou.uh.edu (Roy Christoffersen)
Date: Thu, 20 Jun 1996 14:23:54 -0500
Subject: Acid-Resistant Lacquers for Chemical Thinning

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We have been investigating the possibility of using chemical thinning
methods for some of our thin films, and we understand that the method
requires an acid-resistant mask in the form of a "lacquer". One variety is
called Lacomit but it is not easily available in small quantities or
quickly. Does anyone out there know of a suitable alternative lacquer that
is easily available or a practical supplier of Lacomit?

Thanks

Roy Christoffersen
Texas Center for Superconductivity
3201 Cullen
Houston, TX 77204-5932
roy-at-bayou.uh.edu
(713) 743-8273
FAX: (713) 743-2787






From: Sanford Simon :      simon-at-rockvax.rockefeller.edu
Date: Thu, 20 Jun 1996 16:58:33 -0400
Subject: Acid-Resistant Lacquers for Chemical Thinning

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Message-Id: {2.2.32.19960620205833.006f34d4-at-rockvax.rockefeller.edu}
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Does anyone have experience with any temperature incubators for use on
microscope stages?

Thanks,
Sandy Simon

Sanford M. Simon
Laboratory of Cellular Biophysics
Box 304
Rockefeller University
1230 York Avenue
New York, N.Y. 10021
212-327-8130 (voice)
212-327-8022 (fax)
simon-at-rockvax.rockefeller.edu (e-mail)





From: Murphy, Judy :      murphy-at-sjdccd.cc.ca.us
Date: 20 Jun 1996 14:37:52 -0800
Subject: Philips 100 Available

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Message-ID: {n1376840996.20359-at-sjdccd.cc.ca.us}

Please note, I am writing this note for Dr. Tom McGee, and if you are
interested please contact Tom directly at 800/476-5227. Thanks

Tom has 2, not one, but two Philips' horizontal TEMs they are interested in
getting rid of. If anyone is interested in one or both of these freebies,
please contact Tom McGee at 800/476-5227.

Thanks




From: Radice-at-urvax.urich.edu (Gary Radice)
Date: Thu, 20 Jun 1996 16:48:27 -0400
Subject: Embedding membrane preps

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I agreed to help a colleague thin section a prep of isolated membranes from
intestinal epithelial cells. He says he can pellet them with high-speed
centrifugation. Can anyone point me to a protocol for embedding the pellet
without it falling apart? I only have experience with solid tissue chunks.

Gary Radice 804-289-8107 (voice)
Department of Biology 804-289-8233 (FAX)
University of Richmond
Richmond VA 23173
USA






From: Doug Keene :      DRK-at-shcc.org
Date: Thu, 20 Jun 1996 14:32:35 -0500 (cdt)
Subject: fixation of jelly fish matrix

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Dear Microscopists:

I would not normally ask a question that I could easily
answer by looking at a few papers in the library, but I'm
afraid I am running out of time before I receive a small
number of 1 mm (in size) jelly fish. Does anyone use a
good fixation protocol, resulting in good ultastructure of
marine invertebrates ?

Many thanks,

Doug Keene
Shriners Hospital for Crippled Children
and Marine Invertebrates
----------------------
Doug Keene
DRK-at-shcc.org






From: evagelia-at-rayleigh.lanl.gov (Evagelia Moshopoulou)
Date: Thu, 20 Jun 1996 18:48:35 -0600
Subject: fixation of jelly fish matrix

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unsubscribe
----------------------------------------------------------------------------
------
Evagelia Moshopoulou
Los Alamos National Laboratory,
Condensed Matter and Thermal Physics,
MST-10, MS: K764,
Los Alamos, NM 87545
U.S.A.

tel. (505) 667-9546
fax. (505) 665-7652





From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Thu, 20 Jun 1996 16:35:57 -0700
Subject: TEM: Electron diffraction & image reconstruction

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Message-Id: {v02140b05adef86c897bd-at-[129.82.202.24]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I am looking for a description/discussion of electron diffraction, as it
relates to image reconstruction from diffractograms. This would include
the effects and contributions from 1st, 2nd, 3rd, etc,.... order electrons.

I have been told that there is at least one textbook (} 10 years old) that
has such a discussion, including figures that show reconstructed images
that are different from the original, after removal of various orders of
electrons. This is a graphical demonstration of the contributions of each
order of electron.

I would appreciate any help in finding this information.

John
chandler-at-lamar.ColoState.EDU






From: Hukee, Margaret J. :      hukee.margaret-at-mayo.edu (Marge Hukee)
Date: Thu, 20 Jun 1996 16:46:37 -0500 (CDT)
Subject: Gold Labeling-3

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One uses LM for screening-frozen thicks single day results and can be done
with fluorescent antibodies. paraffin sections withperoxidase she sys that
if it does not work on paraffins it will not work in resins. what resins
have you used how about heat in paraffins. better success with polyclonals
(70%) than monoclonals (15%). brought up the importance of optimizing
secondary antibody.labeling on westerns does not correlate with IEM results
western blot and immunofluorescence before poceeding also tried tissue
blotting

Gold label-3
To summarize from the last post, where I asked about what screening
techniques were being used prior to IEM. Several members mentioned that
they do light microscopy as a screening method using a variety of
techniques. Frozen sections with fluorescent antibodies, paraffin sections
with peroxidase reaction, and tissue blotting have been used. Better IEM
results were reported with polyclonal antibodies (70%) than with monoclonal
antibodies (15%). Some reported that success or failure with western blots
and paraffins did not correlate with results in IEM.

From these replies, another question surfaces. When light microscopy or
western blots results do not correlate with IEM findings, what resin was
used for IEM? Do any of you use light microscopy on resin-embedded tissue
and use polarized microscopy for gold visualization??

One person mentioned the importance of optimizing the secondary
antibody-conjugate. I don't do this. How many feel that this is an
important step in IEM??






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Thu, 20 Jun 1996 18:44:37 -0800
Subject: Re: Acid-Resistant Lacquers for Chemical Thinning

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X-Sender: mager-at-pop.unixg.ubc.ca
Message-Id: {v01510100adefbcb8d62a-at-[137.82.221.121]}
Mime-Version: 1.0
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Dear Roy,
Try nailpolish (colour optional)
Regards,
Mary
} We have been investigating the possibility of using chemical thinning
} methods for some of our thin films, and we understand that the method
} requires an acid-resistant mask in the form of a "lacquer". One variety is
} called Lacomit but it is not easily available in small quantities or
} quickly. Does anyone out there know of a suitable alternative lacquer that
} is easily available or a practical supplier of Lacomit?
}
} Thanks
}
} Roy Christoffersen
} Texas Center for Superconductivity
} 3201 Cullen
} Houston, TX 77204-5932
} roy-at-bayou.uh.edu
} (713) 743-8273
} FAX: (713) 743-2787

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Michael OKeefe
Date: 6/20/96 2:29 PM
Subject: Re: FWD>RE>EM field problem

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Message-Id: {n1376837329.27321-at-macmail.lbl.gov}

Reply to: RE} } FWD} RE} EM field problem


} We have just installed a JEOL 840-F FE-SEM in a lab at IBM-Watson
Research
} Center, and the room has large EM fields that hinder low KeV operation. We
} might try shielding the microscope but have been told that shielding has
not
} been very successful in the past. I was told that there now exists
equipment
} that can sense and compensate for EM fields. Does anyone know who are the
} manufacturers of this type of equipment and if so how well the equipment
works?
} Please respond to: gignac-at-watson.ibm.com or call 914-945-3352.








From: Probing & Structure :      pns-at-ultra.net.au
Date: Fri, 21 Jun 1996 11:00:05 +1000
Subject: solvent cleaning of apertures

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Harold J Crossman wrote:
Regarding the advice to ultrasonically clean in acetone and alcohol....

I would avoid it at all costs. Manufacturer literature, safety manuals, and
common sense dictate that volatile, flammable solvents NOT be used where
there is the possibility for ignition. Since ultrasonic cleaning heats the
solvent, and you probably would not just be standing around watching it
work, you may be inviting disaster. Just think what would happen if you put
a beaker of acetone in an ultrasonic cleaner, forgot about it and went home.

The possibility for fire or explosion is probably small (people
ultrasonically clean in flammable solvents all the time) but the risk should
definitely outweigh the cost of a new aperture.

My advice? Buy new ones.
Harold J. Crossman
OSRAM SYLVANIA INC.
*******************************
In my reply to the original inquiry I had suggested the use of chloroform
(or tri or di-chloroethane). These solvents do not burn unless heated to
very high temperatures. A match may be extinguished in chloroform. I assume
that personnel concerned with instrument maintenance would know that these
solvents are toxic and use a fume hood.
Obviously P&S too supplies apertures, but funds are limited and I hate
waste. The user will have to weigh wasted time versus wasted funds. With
apertures, unfortunately, it is rare that a second or third cleaning attempt
is more successful than the first.

Jim Darley


Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
Internet Catalogue: http://www.ultra.net.au/~pns/





From: Lou Ann Miller :      lmiller-at-ux1.cso.uiuc.edu
Date: Thu, 20 Jun 1996 21:18:47 -0500
Subject: Embedding Membrane Preps

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Message-ID: {31CA0687.68D9-at-ux1.cso.uiuc.edu}

Hi Gary,

I simply process in a 1.5 ml microcentrifuge tube, and spin gently ( or
with the epoxy steps a little less gently to get a pellet) before each
chemical change. If the pellet is large ( more than 1mm deep) I use a
dedicated wooden tool with it's end thined to loosen and break the
pellet when chemical solution is added.

I mix during the process on a rotater bar that turns all the way around
( it has clips to hold the little tubes), so the tubes do need to be
filled almost to the top with each chemical.

At the end, I use itty bitty (000?) single beam capsules, I take off
excess epoxy off the pellet, pipet only a few mm up a dispo pipet at a
time and transfer to the beam capsule, quantitatively transfer as much
as capsules will hold.

--- Then I put the capped beam capsule into a clean 1.5 ml
microcentrifuge tube, and spin fairly hard for 15-20 min. Leave the
beam capsule in the centrifuge tube, and just polymerize for a longer
time, or polymerize till hard, cut out, and then finish polymerization.

** I prefer this because it has better infiltration than my techniques
get with agar. And I can use quicker times with better (?) looking
results.

of course, all is what works for each person in each situation, but
might give this a try....

Lou Ann

} I agreed to help a colleague thin section a prep of isolated membranes from
} intestinal epithelial cells. He says he can pellet them with high-speed
} centrifugation. Can anyone point me to a protocol for embedding the pellet
} without it falling apart? I only have experience with solid tissue chunks.
}
} Gary Radice 804-289-8107 (voice)
} Department of Biology 804-289-8233 (FAX)
} University of Richmond
} Richmond VA 23173
} USA




From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 6/20/96 1:59 PM
Subject: Bubble Analysis

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I have need to investigate bubbles which form between the surfaces of two
borosilicate glasses during a high temperature fusing operation. I would
like to be able to analyze any residual inorganics on the interior surface
of the bubble. We are able to fracture the sample and expose the bubble,
however due to the shape we have been unable to obtain adequate x-ray
signal from within the bubble.

We are also considering analyzing any gases contained in the bubble,
possibly by GC/MS techniques at elevated temperatures. We are concerned
that the material in the bubble may be the same as or similar to that on
the exterior surface.

Does anyone have experience in analyzing bubbles in glass or similar
materials? Suggestions?

Thanks in advance.

Ed Kurz
Institute of Materials Science
University of Connecticut
ekurz-at-mail.ims.uconn.edu








From: Gary Dietrich Chinga :      garyc-at-james.stud.unit.no
Date: Fri, 21 Jun 1996 12:25:12 +0200 (MET DST)
Subject: Re: Confocal Listserver Address

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On Thu, 20 Jun 1996, Richard E. Edelmann wrote:

} Can some one provide me with the listserver address for the confocal
} group?
}
} Thank you.
}
} Richard E. Edelmann
} Electron Microscopy Facility Supervisor

I have another question. I have tried to find out how the confocal works,
Can somebody explain it to me in a simple way. Can confocal be used with
living cells or they have to be fixed and embedded in the first place?

Thanks...


Gary...




From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Fri, 21 Jun 1996 07:42:28 -0300 (EST)
Subject: LM: immunohistochemistry in plastic

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I saw recently a paper describing a interesting method to preserve
lymphocytes antigens in glycol methacrylate embedded tissues with
immnohistochemistry purposes. The method was based in acetone fixation at
room temperature and post-fixation with another liquid (I don't remember
the name, but is not an usual one). Infortunately, I've lost the
reference and I would like to know if a colleague has the number and
the name of the journal where this article was published.
I would be very grateful for your help
=============================================================================
Francisco Javier Hernandez Blazquez |
Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-spider.usp.br
Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265
Departamento de Ciencias Basicas/Histologia| r. 278
Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 618606
CEP 13630-000 Pirassununga (Sao Paulo) |
BRAZIL |
==============================================================================






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Fri, 21 Jun 1996 09:00:21 GMT
Subject: Re: Embedding membrane preps

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} I agreed to help a colleague thin section a prep of isolated membranes from
} intestinal epithelial cells. He says he can pellet them with high-speed
} centrifugation. Can anyone point me to a protocol for embedding the pellet
} without it falling apart? I only have experience with solid tissue chunks.
}
} Gary Radice 804-289-8107 (voice)
} Department of Biology 804-289-8233 (FAX)
} University of Richmond
} Richmond VA 23173
} USA
}
}
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } ..

A high speed pellet will often stay intact after glutaraldehyde fixation, if
andled gently. The are also usually thin enought that they do not need to
be resuspended during each fluid change. Subsequents steps of osmication
and dehydration also firm up the pellet so that it can be left in place
until the fianl embedding. The alternative, for the nervous scientist, is
to suspend the pellet in a small drop of low gelling temp. agarose, chill it
in the frig and then handle the agarose chunk like a piece of tissue. there
are some other approaches as well, but these are the one we routinely use
for this purpose
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: mlamvik-at-mcnc.org
Date: Fri, 21 Jun 1996 10:04:11 -0400
Subject: Do you have a SEM cold stage?

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I'd appreciate hearing from anyone who has a SEM with cold stage that could
be used for observing frozen droplets of an organic liquid.
Thanks,
Michael Lamvik {mlamvik-at-mcnc.org}






From: Hukee, Margaret J. :      hukee.margaret-at-mayo.edu (Marge Hukee)
Date: Fri, 21 Jun 1996 09:55:37 -0500 (CDT)
Subject: apology

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Sorry list members, I left my note sheet attached to my last post, please
disregard the first paragraph?? Thanks
Marge






From: Radice-at-urvax.urich.edu (Gary Radice)
Date: Fri, 21 Jun 1996 11:00:48 -0400
Subject: Embedding membrane vesicles

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Thanks to all who responded to my request for tips on embedding plasma
membrane pellets. I have enough suggestions to get started.

Gary Radice 804-289-8107 (voice)
Department of Biology 804-289-8233 (FAX)
University of Richmond
Richmond VA 23173
USA






From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Fri, 21 Jun 1996 11:30:25 -500
Subject: RE: Confocal Listserve - Thank you

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Thank you to all who replied. I have the needed info. (Please,
stop sending me more responses).

For those of you who asked me to forward the info along here it is:

To subscribe to the confocal listserver send the foolowing e-mial
message:

TO: listserv-at-ubvm.cc.buffalo.edu

(Text body:) subscribe confocal


----+-----+-----


You will receive a couple of confirmation messages.

To send messages to the confocal listserver group the address is:

Confocal-at-ubvm.cc.buffalo.edu



Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu




From: JOHNA-at-SCI.WFBR.EDU
Date: Fri, 21 Jun 1996 09:47:53 -0500 (EST)
Subject: Size standards

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Hello folks,

I'll be doing some negative staining of macromolecules soon and was wondering
if you all would suggest a good internal size standard to add to the specimens
to aid in determining the dimensions of these puppies.

TIA

John A

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Biomedical Research |
| 222 Maple Avenue |
| Shrewsbury, MA 01545 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfbr.edu |
| |
|_________________________________________________|





From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 21 Jun 96 10:25:02 EDT
Subject: Acid Resistant Lacquer

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Roy:

There is a material called Microshield Lacquer that we used to supply with our
Model 550 Single Vertical-Jet Polisher. I checked our stock and we no longer
have any (although we do still the Model 550!). You can purchase the lacquer
from the manufacturer (or at least you could a few years ago!) at:

Pyramid Plastics
Tolber Division
220 W. 5th Street
Hope, Arkansas 71801

TEL: 501-777-3251

I understand that they are a subsidiary of Michigan Chrome and Chemical.

The Lacomit varnish I'm not sure about. You may want to try a company like SPI
Supplies. You can check ot their home page at http://www.2spi.com or e-mail to
them at spi2spi-at-2spi.com. I'm not certain if they have it, but they have a
pretty wide range of materials and may be able to help you.

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
http://www.southbaytech.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: JOHNA-at-SCI.WFBR.EDU
Date: Fri, 21 Jun 1996 09:37:48 -0500 (EST)
Subject: Re: Embedding membrane preps

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Hi Gary,

High speed spun membrane preps are easy; they generally either stick to the
bottom of the tube throughout processing or, if they release from the tube,
they usually remain intact or a couple of pieces. Treat them as you would a
piece of tissue. If they're very small, they usually are, just a bit of scum
on the bottom of they tube, you can reduce the times spent in your various
fixatives/washes/etc.

Hope this helps. Good Luck.

John A.

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Biomedical Research |
| 222 Maple Avenue |
| Shrewsbury, MA 01545 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfbr.edu |
| |
|_________________________________________________|





From: garyc-at-stud.unit.no (Gary)
Date: Fri, 21 Jun 1996 19:34:19 +0200 (MET DST)
Subject: Reference marks

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I have received the following suggestions:

1.- put a hair ot thread in the block to align serial sections.
2.- trim block faces in the normal trapezoid shape, with one side cut
close to 90
degrees, (squared-off) made for a kind of pointer on the one side. As long as I
kept that pointed side of the section toward me and to the left (in my
particular case) I knew that I had the proper orientation.

3.- In the past we have used the outside shape of the block to orient serial
sections.

Well, thanks for your suggestions.

If somebody has another method so let me know.


/////////////////////////////////////////////////////////////////
// // //

// Gary Chinga // email :garyc-at-james.avh.unit.no //
// Plantebiosenteret // WWW :http://www.nvg.unit.no/~gary //
// NTNU, 7055 Dragvoll // phone : 73590168 //
// Norway // fax : 73590177 //
// // //
/////////////////////////////////////////////////////////////////






From: Neal Nicklaus :      nnicklaus-at-nova.sarnoff.com
Date: Fri, 21 Jun 1996 10:02:39 -0400
Subject: Reference marks

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Message-Id: {199606211405.KAA13119-at-vaxserv}
X-Sender: nnicklaus-at-cave.sarnoff.com
X-Mailer: Windows Eudora Pro Version 2.1.2
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Michel's points,see below are well taken. Many older VCRs did not have
stable sync signals. Another video device might not be able to get a clear
signal. Newer VCRs are generally much better. However, if you have a
problem, you might be able to playback in a "normal" VCR which might have
editing capabilities. If required, you can buy a sync generator for use in
a system, but you will need help getting all your components set up
properly. There may be someone at your facility who could help. Otherwise,
a GOOD sales engineer or techncal support person might walk you through the
process.

-------
} Michel Deschuyteneer {deschuyt-at-sbbio.be} wrote:

} First, we could not get a clear signal to grab frames in our image analyzer
} (some frequency problem) for either morphometry or just illustration. Make
} sure that the system you get works with any other component (e.g.
} videoprinter) you consider attaching it to fixing this kind of
} incompatibility is always possible but may be expensive..
} Second, there were no editing capabilities which you may find on some top
} of the line models. Compiling sequences for presentation was difficult,
} short of going to a professional (read expensive) studio. I assume that
} there are nowadays relatively affordable solutions.

Neal Nicklaus

SEQ Limited

Voice: 609-452-6033 Ext. 13
Fax: 609-452-5955
email nnicklaus-at-seq.sarnoff.com





From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Fri, 21 Jun 1996 10:28:55 -0500
Subject: Re: Catalese problems

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In message {960613154016_327266792-at-emout15.mail.aol.com} writes:
} Greetings everyone:
} We recently purchased a Catalese TEM grid from a commercial supplier. It was
} to be used to check-out the resolution of our two microscopes (a Zeiss
} EM109, and Siemens 101). We had difficulty seeing any periodicity at all
} in the Siemens, although we searched for about one hour. The Zeiss
} revealed photographable and measure periodicity in approximately 1 in 100
} crystals. We returned the grid to the Siemens, but still could not fined any
} usable crystals. Before we determined the Seimens had poor resolution, we
} returned the grid to the Zeiss--but to our shock, surprise, chagrin, and
} consternation--could find *no* good crystals!
} Questions:
} 1) Are these crystals sensitive to damage from the beam (like contamination)?
} 2) Would a carbon coat benefit catalese grids purchased in the future?
} 3) For that matter, is there is anything better than catalese?
} Thanx
} cya,
} Gene


Sorry for the late reply. I've looked at catalase crystals (from Ted Pella, Inc,
#612) on two different scopes, Philips EM300 & CM-12, and obtained excellent
images that were very stable over time. Perhaps you got a bad grid from your
supplier, but assuming the sample is OK, I would ask what operating conditions
you were using on your scopes? In particular, what diameter objective &
condensor apertures were you using?

I'll never forget the pleasant shock I got when I found out the effect that
condenser aperture diameter can have on resolution at the mags you would view
catalase crystals at. I used to think that the condensor aperture was just a
coarse brightness control, a big valve on the electron flow. But using a 50 to
100 micron condenser aperture can show big improvement in resolution over a 200
micron size. I've tried to find out why in books on TEM optics theory but the
turf is fraught with mathematical complexities, contrast transfer functions and
stuff like that. Can anyone out there give it to me straight?

Of course, objective aperture diameter affects contrast of image too. I
typically use a 40, sometimes a 20 micron diameter objective aperture for
viewing bilogical sections. There is probably an optimum combination of the two
aperture sizes to maximize resolution as a funtion of the other operating
values, kV, spot size, sample type, etc.

This is basic stuff that most of us at least have a feel for, but I sometimes
don't fully understand the "why" of it, like the condenser aperture effect.

Anybody out there want to chime in with their 2 cents worth on this topic?


Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: Probing & Structure :      pns-at-ultra.net.au
Date: Sat, 22 Jun 1996 18:46:18 +1000
Subject: Embedding membrane preps

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} Garry Radice wrote:
} I agreed to help a colleague thin section a prep of isolated membranes from
} intestinal epithelial cells. He says he can pellet them with high-speed
} centrifugation. Can anyone point me to a protocol for embedding the pellet
} without it falling apart? I only have experience with solid tissue chunks.
}
} Gary Radice 804-289-8107 (voice)
} Department of Biology 804-289-8233 (FAX)
} University of Richmond
} Richmond VA 23173
} USA
} ***********************
Many people had that problem and it is worth posting.
The trick is: Fix the pellet (max 1mm thick) for five minutes. Score pellet
with a needle into 1mm squares, continue fixation as if they were tissue
blocks; just exchange fluids more gently.

Material fixed in suspension must be spun every step along the protocol.
Initial pellet fixation will keep the material together. Note: Start with
at least a half mm pellet. Plenty of material to start with will result in
more than enough in the end. Start with minimal material and somehow you'll
finish up with nothing.

Jim Darley


}
}
Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site http://www.ultra.net.au/~pns/
***********************





From: Sarka Lhotak :      lhotaks-at-fhs.csu.mcmaster.ca
Date: Sat, 22 Jun 1996 08:18:25 -0400 (EDT)
Subject: TEM - immunogold as a service

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Dear Margaret,
For IEM, I use LR White routinely, polymerized with UV light in
-20 C freezer. However, since polymerization of acrylics is highly
exothermic, I am sure that the actual temperature of the tissue gets much
higher, though surely not as high as paraffin.
The problem is, that one cannot predict for sure how the next
antigen-antibody combination will work. Eg., I stained neural protein
GAP-43. It worked very nicely on paraffin/peroxidase, but at EM level on
LR White sections I could obtain staining only with 5nm gold (silver
enhanced), but absolutely nothing with 10 nm gold (published in
Histochem.J.27,272-279,1995, there are some interesting correlations with
LM there also). This was the only case of such a clear-cut sterical
hindrance that I have encountered so far after staining close to 200
antibodies. The question is, how do you charge people for a work like
this? It takes a lot of experimentation to find this out. Therefore, I
think that your effort to establish some criteria for the acceptance of
projects is very wise. The existence of such criteria will also emphasize
to your customers the fact that this is a custom work and the obtaining of
positive results cannot be a priori guaranteed.
Thin cryosectioning with subsequent immunolabelling and
"embedding" of sections either in methylcellulose or diluted plastic is a
good technique for cell culture pellets or specimens that do not require
"blue" sections for localization of the site of interest. It is fairly
rapid (no tissue processing), and the antigen should be well preserved.
I don't like preembedding techniques because individual cell
components are not all equally accesible to reagents as in a section,
therefore the interpretation of results is much more difficult.
Thick LR White sections can be stained with gold and silver
enhanced for LM. I don't think the secondaries need to be optimized in
each staining, we only check this with a new batch etc.
Is anybody actually providing IEM as a service?
Your sincerely,

Sarka Lhotak, lhotaks-at-fhs.mcmaster.ca

EM Facility, McMaster Univerity
Hamilton, Ontario, Canada







From: zaluzec-at-sparc5.microscopy.com (Nestor J. Zaluzec)
Date: Sun, 23 Jun 1996 17:22:40 -0500
Subject: MSA Membership Directory Now-OnLine

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Message-Id: {199606232218.RAA02343-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
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G'day Colleagues...

I have completed yet another bit of WWW information upgrading
on the MSA WWW site

http://www.msa.microscopy.com

The MSA Membership Directory is now a searchable index on-line.
You may search for member information by First/Last Name and will
be returned with their Affiliation/Address/Phone/Fax/Email information,
as listed in the Membership Directory. The database reflects my
records of member information as of April 3, 1996.

Please note that Corporate/Sustaining Members of the Society are also
listed in this Searchable Index, and can be found by entering
their proper company name. Or you may choose to look at the Sustaining
Members home pages also on the MSA Site.

Currently the index is searchable only by Member Name. It is also
limited
to presenting the first "10" hits in the database. So choose your
search criteria carefully! As I get "free" time I will expand the
search/match criteria.

If you find that your information is out-of-date, then you may
use the Electronic Membership Form which is also available on-line
to update your records at the Business Office. The on-line
database will be updated at regular intervals (~ quarterly).

BTW. Complete downloads of the MSA Database are NOT permited so
don't bother to try or ask....

Cheers.... Nestor
Your Friendly Neighborhood SysOp







From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Mon, 24 Jun 1996 08:29:43 +0100 (BST)
Subject: Re: Do you have a SEM cold stage?

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Dear Mike:

Yes, we have an Oxford Cold Stage on the side of an XL30 Hot FEG plus and
Isis Germanium ED system. You are very welcomemto come and use it

Patrick Echlin
Director, Multi-Imaging Centre
University of Cambridge

On Fri, 21
Jun 1996 mlamvik-at-mcnc.org wrote:

} I'd appreciate hearing from anyone who has a SEM with cold stage that could
} be used for observing frozen droplets of an organic liquid.
} Thanks,
} Michael Lamvik {mlamvik-at-mcnc.org}
}
}
}




From: Jouko =?iso-8859-1?Q?M=E4ki?= :      jouko.maki-at-utu.fi
Date: Mon, 24 Jun 1996 12:45:19 +0300
Subject: www-address changed, sorry

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Dear All,

Our computer center has changed the location of our www-pages to:

http://www.utu.fi/med/tdk/em/index.html

Sorry for the trouble.

Jouko

Jouko K. M=E4ki, Ph.D., Laboratory Manager
University of Turku, Laboratory of Electron Microscopy
Kiinamyllynkatu 10 FIN-20520 TURKU FINLAND
Tel: +358 21 333 7318 GSM: +358 40 505 2521 FAX: +358 21 333 7380





From: BHULL-at-desire.wright.edu
Date: Mon, 24 Jun 1996 08:39:10 -0500 (EST)
Subject: subscription

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I have received the initial message about the MSA service.
Barb Hull, BHULL-at-desire.wright.edu




From: ebs-at-ebsciences.com
Date: Mon, 24 Jun 1996 10:11:28 -0500
Subject: Re: Acid-Resistant Lacquers for Chemical Thinning

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Roy-

Lacomit varnish is commonly used for the preparation of thinned specimens by
one of the so-called "window" techniques. The area of the specimen not
required for polishing is coated with the varnish.

The product was in the old BioRad catalog, and has been taken over by Energy
Beam Sciences (catalog #A0944, 500ml bottle).

Best regards,
Sonja L. White, Sales & Marketing Secretary
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Mon, 24 Jun 1996 10:53:13 -0500
Subject: ISI DS-130 diffusion pumps

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Message-Id: {199606241548.KAA11891-at-ux1.cso.uiuc.edu}
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Does anyone know of a source for used ISI DS-130 diffusion pumps,
or have one to maybe sell?

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
soon-to-be-closed
Center for Electron Microscopy
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********






From: Jane A. Fagerland (847) 935-0104 :      FAGERLAND.JANE-at-igate.pprd.abbott.com
Date: Mon, 24 Jun 1996 08:17:00 -0500 (CDT)
Subject: LM: immunohistochemistry in plastic - reference

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Mr-Received: by mta RANDB; Relayed; Mon, 24 Jun 1996 08:34:37 -0500
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In response to the request for a reference for IHC in methacrylate
sections:

The following paper describes a fixation method using acetone containing
protease inhibitors, followed by pretreatment with methyl benzoate prior
to infiltration with GMA. The authors used this method to successfully
label CD3, CD4, CD8, CD20, CD45 lymphocytes, as well as a variety of
other leukocyte antigens. It also worked for labeling several types of
integrins, collagens, and other matrix proteins.

Britten, Karen M., Howarth P.H., and Roche W.R. 1993:
Immunohistochemistry on resin sections: a comparison of resin embedding
techniques for small mucosal biopsies. Biotechnic and Histochemistry
68(5):271-280.

Hope this helps!

Jane A. Fagerland, Ph.D.
Dept. Microscopy and Microanalysis
Abbott Laboratories
Abbott Park IL 60064






From: ychen14-at-facstaff.wisc.edu
Date: Mon, 24 Jun 1996 12:09:42 -0700
Subject: LM: immunohistochemistry in plastic - reference

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Dear Netters,

Does anyone know the address or email address of Dr. R. Autrata. I
appreciate your help.
Regards,

Ya Chen


Ya Chen

*** My email address has been changed to: ychen14-at-facstaff.wisc.edu ***
==========================================================================
Cryo/SEM Coordinator
Integrated Microscopy Resource (IMR)-- III M M RRRRRR
an NIH Biomedical Research Resource I M M M M R R
University of Wisconsin, Madison, WI I M M M RRRRRR
1675 Observatory Drive #167 I M M R R
Madison, WI 53706, USA I M M R R
TEL : 608-263-8481 I M M R R
FAX : 608-265-4076 III M M R R
Email:ychen14-at-facstaff.wisc.edu
==========================================================================
IMR WWW Home Page: http://www.bocklabs.wisc.edu/imr.html
2nd Symposium on Integrated Microscopy: Sept. 20-22, 1996






From: ychen-at-MACC.WISC.EDU
Date: Mon, 24 Jun 1996 12:04:10 -0700
Subject: Re: Do you have a SEM cold stage?

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Message-Id: {v02120d02adf486f689db-at-[144.92.132.30]}

On Fri, 21
Jun 1996 mlamvik-at-mcnc.org wrote:

} I'd appreciate hearing from anyone who has a SEM with cold stage that could
} be used for observing frozen droplets of an organic liquid.
} Thanks,
} Michael Lamvik {mlamvik-at-mcnc.org}
}
}


Dear Mike:

Yes, we do. There is a Hitachi S-900 "in-lens" type of FESEM with a Gatan
cold-stage (type 626) at the Integrated Microscopy Resource, Madison,
Wisconsin. You can look at the images obtained from this high resolution
SEM and cold-stage from our Web site:
http://www.bocklabs.wisc.edu/imr.html.

If you are interested it, you are welcome to use it.

Regards,

Ya Chen


Ya Chen

*** My email address has been changed to: ychen14-at-facstaff.wisc.edu ***
==========================================================================
Cryo/SEM Coordinator
Integrated Microscopy Resource (IMR)-- III M M RRRRRR
an NIH Biomedical Research Resource I M M M M R R
University of Wisconsin, Madison, WI I M M M RRRRRR
1675 Observatory Drive #167 I M M R R
Madison, WI 53706, USA I M M R R
TEL : 608-263-8481 I M M R R
FAX : 608-265-4076 III M M R R
Email:ychen14-at-facstaff.wisc.edu
==========================================================================
IMR WWW Home Page: http://www.bocklabs.wisc.edu/imr.html
2nd Symposium on Integrated Microscopy: Sept. 20-22, 1996






From: kennedy-at-nsi.edu (grace kennedy)
Date: Mon, 24 Jun 1996 13:21:26 -0800
Subject: IHC in plastic-reference

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I came across this paper some time ago-material is fixed in PFA, processed
cold, taken through ammonium chloride, dehydrated in acetone and embedded
in glycol methacrylate. Approximately 40 antigens were successfully
labeled using a trypsin pretreatment.

Beckstead, Jay H: Optimal ANtigien Localization in Human Tissues Using
Aldehyde-fixed Plastic-embedded Sections. J Histochem Cytochem 33:954-958,
1985






From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Mon, 24 Jun 1996 10:53:13 -0500
Subject: ISI DS-130 diffusion pumps

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Message-ID: {n1376496826.81053-at-mse.engin.umich.edu}
"philip oshel" {oshel-at-ux1.cso.uiuc.edu}
X-Mailer: Mail*Link SMTP/QM 3.0.0GM

Reply to: RE} ISI DS-130 diffusion pumps

As I mentioned previously, The Duniway Sstockroom Corp. (1305 Space Park
Way, Mountain View, CA 94043; Ph. 800-446-8811; Fx: 415-965-0764) handles
new, reconditioned, surplus, and replacement vacuum parts and equipment. I
would recommend contacting them as a possible source of help in obtaining a
pump for your application.
W. C. Bigelow (bigelow-at-umich.edu)

--------------------------------------
Does anyone know of a source for used ISI DS-130 diffusion pumps,
or have one to maybe sell?

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
soon-to-be-closed
Center for Electron Microscopy
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********



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From: kszaruba-at-MMM.COM (Karen S. Zaruba)
Date: Mon, 24 Jun 1996 17:17:11 -0500
Subject: LM - Need parts for Zeiss scope

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Does anyone know of a low cost source for a beam stop and a 40x dry (high
NA but no need for phase contrast, etc) objective for a Zeiss microscope? I
need these items but they are not a big enough priority to pay for brand new
ones (if they were, our local Zeiss rep is very good so I do not need references
to other dealers.)

In general any source of used Light Microscopy equipment may be useful.

Thanks in advance!

Karen Zaruba

Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
3M Company
3M Center 270-1S-01 Phone: 612-737-2971
St. Paul, MN 55144-1000

These opinions are my own and may not represent those of 3M.







From: D.Cousens-at-mailbox.uq.oz.au (Dr David Cousens)
Date: Tue, 25 Jun 1996 17:42:57 +1000
Subject: Subscribe

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SUBSCRIBE
Dr David R. Cousens
Senior Research Fellow Ph 61-7-33654947
Centre for Microscpy and Microanalysis Fax 61-7-33651775
The University of Queensland Email
D.Cousens-at-mailbox.uq.oz.au
St Lucia 4072 AUSTRALIA





From: EvexAnalyt-at-aol.com
Date: Tue, 25 Jun 1996 08:44:07 -0400
Subject: Re: EDX: TN 5500

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Evex apologizes for its unintentional commercial use of the microscopy list
server.

Regards
Peter Tarquinio
President
Evex Analytical




From: Dennis B. Barr :      dennbarr-at-eastman.com
Date: Tue, 25 Jun 1996 08:18:52 -0400
Subject: Re: LM - Need parts for Zeiss scope

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} In general any source of used Light Microscopy equipment may be useful.
}
} Thanks in advance!
}
} Karen Zaruba
}
===========================================================}

You might find what you need from Spectra Services, Inc. in Rochester New
York. (716)654-9500 and ask for Mike Specht. I purchased a used rotating
stage from them a few months ago.
Dr. Dennis B. Barr
Eastman Chemical Company
Microscopy and Morphology Research Laboratory
P.O. Box 1972
Kingsport, TN 37662-5150

Voice: 423/229-2188
E-mail: dennbarr-at-eastman.com
FAX: 423/229-4558






From: spinka-at-uete.fee.vutbr.cz
Date: Tue, 25 Jun 1996 15:14:09 MET-1MEST
Subject: Re:

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Address

Prof. Rudolf Autrata
director
UPT AV CR Tel.+42 - 5 - 41321246
Kralovopolska 147 Fax.+42 - 5 - 41211168
612 64 BRNO e-mail: autrata-at-isibrno.cz
CZECH REPUBLIC

} From: ychen14-at-facstaff.wisc.edu
} Date sent: Mon, 24 Jun 1996 12:09:42 -0700
} To: Microscopy-at-Sparc5.Microscopy.Com

} Dear Netters,
}
} Does anyone know the address or email address of Dr. R. Autrata. I
} appreciate your help.
} Regards,
}
} Ya Chen
}
}
} Ya Chen
}
} *** My email address has been changed to: ychen14-at-facstaff.wisc.edu ***
} ==========================================================================
} Cryo/SEM Coordinator
} Integrated Microscopy Resource (IMR)-- III M M RRRRRR
} an NIH Biomedical Research Resource I M M M M R R
} University of Wisconsin, Madison, WI I M M M RRRRRR
} 1675 Observatory Drive #167 I M M R R
} Madison, WI 53706, USA I M M R R
} TEL : 608-263-8481 I M M R R
} FAX : 608-265-4076 III M M R R
} Email:ychen14-at-facstaff.wisc.edu
} ==========================================================================
} IMR WWW Home Page: http://www.bocklabs.wisc.edu/imr.html
} 2nd Symposium on Integrated Microscopy: Sept. 20-22, 1996
}
}
}
Sincerely,

===========================================================
Jiri Spinka
Faculty of Electrical Engineering and Computer Science
Department of Electrotechnology
Technical University of Brno EEEEEE TTTTTT
Antoninska 1, B R N O EE TT
Czech Republic EEEE TT
Tel. 42-5-753741, Fax. 42-5-41211135 EE TT
e-mail: spinka-at-uete.fee.vutbr.cz (Internet) EEEEEE(hi) TT
===========================================================




From: Adel :      adel-at-zirc-mms.chem-eng.toronto.edu
Date: Tue, 25 Jun 1996 09:53:14 -0500 (CDT)
Subject: Re:

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Subscibe Microscopy




From: wise-at-vaxa.cis.uwosh.edu
Date: Tue, 25 Jun 1996 11:57:26 +0000
Subject: film resolution

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To all:

I was recently asked the following question about film resolution. I have
seen a discussion of this somewhere but can't seem to locate it now. Can
anyone point me in the right direction?

Bob Wise

***************

"What is the max resolution of the EM film you used to use? [This was Kodak
EM film no. 4489, Estar thick base] In particular, how close can two lines
be and still be distinguished, or how thin can a line be and still be
detected. Also I want to know how many photograins it takes to generate
this level of resolution. For example, on a 2" x 3" film, the smallest dot
that can be detected has an area of x sq. inches and this area on the film
contains on average y silver grains."

Can you give me any help in answering this? Is there some data on the size
and concentration per unit area of grains in various films?







From: Susanne Pignolet Brandom :      spb-at-wwa.com
Date: Tue, 25 Jun 1996 10:12:53 -0500
Subject: Re: LM - Need parts for Zeiss scope

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Message-Id: {2.2.32.19960625151253.0068ae6c-at-pop.wwa.com}
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Karen

Dealers of new optical equipment are often the best source of used
equipment, especially accessories. They often take microscopes in on trade
or pick up extra components when a product is discontinued. I have
purchased used Orthoplan accessories from my local Leica dealer and they are
always clean and in good condition. In addition, the rep has brought me the
pieces and made sure that they worked on my microscope. If your local dealer
does not have the used accessories you are looking for, ask him if he knows
of a good source. Microscope dealers trade equipment. Zeiss should also
have a list.

Also try your local Leica, Nikon, and Olympus dealers.

There is a list of used equipment dealers with WWW sites at
http://www.mwrn.com/product/
All of these carry optical or electron microscopes and accessories.
However, some of these will not break up a microscope that has the component
you want. Depending on your needs, it can be cheaper to buy a whole used
system.

Susanne Pignolet Brandom
MC Services
MicroWorld Resources and News
http://www.mwrn.com/

At 05:17 PM 6/24/96 -0500, you wrote:
} Does anyone know of a low cost source for a beam stop and a 40x dry (high
} NA but no need for phase contrast, etc) objective for a Zeiss microscope? I
} need these items but they are not a big enough priority to pay for brand new
} ones (if they were, our local Zeiss rep is very good so I do not need
references
} to other dealers.)
}
} In general any source of used Light Microscopy equipment may be useful.
}
} Thanks in advance!
}
} Karen Zaruba
}
} Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
} 3M Company
} 3M Center 270-1S-01 Phone: 612-737-2971
} St. Paul, MN 55144-1000
}
} These opinions are my own and may not represent those of 3M.
}
}
}
}
}





From: Adel :      adel-at-zirc-mms.chem-eng.toronto.edu
Date: Tue, 25 Jun 1996 09:55:06 -0500 (CDT)
Subject: Computer Tech Support Problem to Avoid

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
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Disclose-Recipients: prohibited
Conversion: allowed
Importance: normal
Priority: normal
Sensitivity: Company-Confidential


Subscribe Microscopy




From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Tue, 25 Jun 1996 19:26:12 -0300 (EST)
Subject: Re: LM: immunohistochemistry in plastic - reference

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On Mon, 24 Jun 1996, Jane A. Fagerland (847) 935-0104 wrote:

} Britten, Karen M., Howarth P.H., and Roche W.R. 1993:
} Immunohistochemistry on resin sections: a comparison of resin embedding
} techniques for small mucosal biopsies. Biotechnic and Histochemistry
} 68(5):271-280.
}
} Hope this helps!
}
} Jane A. Fagerland, Ph.D.
} Dept. Microscopy and Microanalysis
} Abbott Laboratories
} Abbott Park IL 60064

Sure it will!
Thank you very much....again!
=============================================================================
Francisco Javier Hernandez Blazquez |
Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-spider.usp.br
Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265
Departamento de Ciencias Basicas/Histologia| r. 278
Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 618606
CEP 13630-000 Pirassununga (Sao Paulo) |
BRAZIL |
==============================================================================







From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Tue, 25 Jun 1996 18:30:57 -0800
Subject: Re: film resolution

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Dear Bob,
I recall John Stevens at the University of Toronto giving a lecture on this
at a MSC meeting several years ago. He was advocating digital imaging but
warning that the digital resolution necessary to equal film required file
sizes in the order of a gigabite. This is when gigabite files were
considered completely unattainable. I believe the figures were 1000 X 1000
in a 3.5" by 4.5" film. I have lost touch, but he was attached to the
Medical School there. There may be a discussion in the MSC Proceedings. I
believe that meeting was in Winnipeg.
Regards,
Mary
} To all:
}
} I was recently asked the following question about film resolution. I have
} seen a discussion of this somewhere but can't seem to locate it now. Can
} anyone point me in the right direction?
}
} Bob Wise
}
} ***************
}
} "What is the max resolution of the EM film you used to use? [This was Kodak
} EM film no. 4489, Estar thick base] In particular, how close can two lines
} be and still be distinguished, or how thin can a line be and still be
} detected. Also I want to know how many photograins it takes to generate
} this level of resolution. For example, on a 2" x 3" film, the smallest dot
} that can be detected has an area of x sq. inches and this area on the film
} contains on average y silver grains."
}
} Can you give me any help in answering this? Is there some data on the size
} and concentration per unit area of grains in various films?

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Koenraad.Janssens-at-mtm.kuleuven.ac.be (Koenraad Janssens)
Date: Wed, 26 Jun 1996 07:54:54 +0200
Subject: Re: film resolution

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} Bob Wise wrote:
}
}
} ....
} "What is the max resolution of the EM film you used to use? ....

If you imply TEM plate film, I seem to remember that one can get something
like 10000 by 8000 "pixels" resolved on a standard plate size.

___________________________________________________________________ _ _ _
___________________________________________________________________ _ _ _

Koenraad Janssens, Ph.D.

KULeuven
Department of Metallurgy and Materials Engineering (MTM)
de Croylaan 2, B-3001 Leuven, Belgium
Tel. : +32-(0)16-32.1232
Fax : +32-(0)16-32.1992
e-mail : Koenraad.Janssens-at-mtm.kuleuven.ac.be





From: Probing & Structure :      pns-at-ultra.net.au
Date: Wed, 26 Jun 1996 16:31:43 +1000
Subject: film resolution, longish

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} From: wise-at-vaxa.cis.uwosh.edu
} Date: Tue, 25 Jun 1996 11:57:26 +0000
} Subject: film resolution
}
} To all:
} I was recently asked the following question about film resolution. I have
} seen a discussion of this somewhere but can't seem to locate it now. Can
} anyone point me in the right direction?
} Bob Wise
} } ***************
} "What is the max resolution of the EM film you used to use? [This was Kodak
} EM film no. 4489, Estar thick base] In particular, how close can two lines
} be and still be distinguished, or how thin can a line be and still be
} detected. Also I want to know how many photograins it takes to generate
} this level of resolution. For example, on a 2" x 3" film, the smallest dot
} that can be detected has an area of x sq. inches and this area on the film
} contains on average y silver grains."
}
} Can you give me any help in answering this? Is there some data on the size
} and concentration per unit area of grains in various films?
********************************
reply: The questions Bob is asking are interesting and I expect that he
will receive some direct answers. But there are some related points which
should be looked at in this context, particularly when analogies to digital
imaging are to be drawn.
Line resolution of any film suitable for TEM is better than 200/mm. In TEM
it is desirable to maximise electrons for exposure, this will assure better,
less grainy, more contrasty and better resolved images. Slight over-exposure
and a very slow film type are in fact desirable. This is fortuitous: The
TEM's requirements make the highest resolution and most contrasty emulsions
the most suitable.
In general terms, resolution of a TEM is equal at all magnifications but a
low power image would require enlarging. At about 30x photographic
magnification, insufficient electrons have formed the image and "noise"
becomes intolerable. Also, above 15x and certainly by 20x, the photographic
enlarging procedure becomes quite impractical.
There is a very good reason to use fairly high, perhaps 10x to 15x,
photographic enlargements: The depth-of-field (or focus) is much greater at
lower magnifications. It is much easier to take an in-focus picture at 20k
than at 70k. Enlarging to 200k will give "identical images", but the 70k
image is much more likely to be out of focus. Anybody who frequently
requires very high magnification TEM images would appreciate that
depth-of-field is a powerful argument.
The corollary is: Except for giant enlargements, only a small postage stamp
size area from the centre of the negative is normally used. There is also a
not very subliminal message here.
The question now is: Which digital system maintains the huge advantages of
low power depth-of-field and has the resolution to allow 20x enlargement, if
only from a postage stamp sized area?
SEMs and light microscopes are another discussion and reasons for digital
images from these are well advanced.
It should be noted that P&S has an interest in film, digital cameras and
printers. - We like them all!

Jim Darley

Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site http://www.ultra.net.au/~pns/
***********************





From: bruyntjes-at-hvvc03.voeding.tno.nl
Date: Wed, 26 Jun 1996 12:21:20 EDT
Subject: mucosal mastcells

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microscopy {Microscopy-at-Sparc5.Microscopy.Com}

Hello everybody

Is there anybody familiar with a technique to stain mucosal mast cells in
the intestines of the rat on cryosections and paraffin-embedded material.

Thanks





From: chris gilpin :      cgilpin-at-fs1.sem.man.ac.uk
Date: Wed, 26 Jun 1996 11:34:21 BST
Subject: Image archiving software

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Now that digital imaging is with us what are people using for
archiving and databasing their images.
I am looking for info on commercially available software. Any
thoughts would be most appreciated.

Raining in Manchester!!

Chris

Chris Gilpin
Biological Sciences Electron Microscope Unit
G452 Stopford Building
Oxford Road
Manchester
M13 9PT
phone +44 161 275 5170
fax +44 161 275 5171




From: preid-at-rsmas.miami.edu (Pamela Reid)
Date: Wed, 26 Jun 1996 08:57:15 -0500
Subject: subscribe

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subscribe

__________
Dr. Pamela Reid
Research Associate Professor
University of Miami/RSMAS-MGG
4600 Rickenbacker Causeway
Miami, Fl 33149

email: preid-at-rsmas.miami.edu
phone (305) 361-4606
fax (305) 361-4632






From: Sara Miller :      saram-at-acpub.duke.edu
Date: Wed, 26 Jun 1996 09:28:41 -0400 (EDT)
Subject: Re: film resolution

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I can't answer your question directly, but the Kodak tech reps are
usually pretty helpful. Their number is 800 225 5352. AGFA's number is
201 641 9566. Hope this helps.
S. Miller

On Tue, 25 Jun 1996 wise-at-vaxa.cis.uwosh.edu wrote:

} Date: Tue, 25 Jun 1996 11:57:26 +0000
} From: wise-at-vaxa.cis.uwosh.edu
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: film resolution
}
} To all:
}
} I was recently asked the following question about film resolution. I have
} seen a discussion of this somewhere but can't seem to locate it now. Can
} anyone point me in the right direction?
}
} Bob Wise
}
} ***************
}
} "What is the max resolution of the EM film you used to use? [This was Kodak
} EM film no. 4489, Estar thick base] In particular, how close can two lines
} be and still be distinguished, or how thin can a line be and still be
} detected. Also I want to know how many photograins it takes to generate
} this level of resolution. For example, on a 2" x 3" film, the smallest dot
} that can be detected has an area of x sq. inches and this area on the film
} contains on average y silver grains."
}
} Can you give me any help in answering this? Is there some data on the size
} and concentration per unit area of grains in various films?
}
}
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: Wei_Ging :      cfs_wei-at-dsapp1.hmi.de
Date: Wed, 26 Jun 1996 17:19:05 +0200
Subject: Phase Identification problem

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Need help for identifying the crystall structure of an unknown phase. A series
of SAD patterns have been got. One of them has 6P symmetry in ZOLZ pattern.As a
rusult of computer simmulation , the possibility of having cubic or hexagonal
structure has been excluded.So the most possible structure the phase has is
rhombohedra. Who is familiar with this structure and knows some suitble
simmulationsprogram?
Thanks
E-mail address:cfs_wei-at-nsun1.hmi.de


Q.Wei






From: Wei_Ging :      cfs_wei-at-dsapp1.hmi.de
Date: Wed, 26 Jun 1996 16:45:58 +0200
Subject: Fourier transformation programm

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Dear Colleague:
Our selected area electron diffraction pattern from a amorphous were recorded
on the IP(Imagine-Plate), we want to calculate the radial distribution
function(RDF) from the intensity measurements by the Fourier transformation of
the coherently scattered electron intensity. However, we don't have the
transformation programm, could you give us some information about the now
available program? In addition, can anyone give us a suggestion how can we
measure the intensity from the IP exactly. Thanks.
our email address: cfs_wei-at-nsun1.hme.de





From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Wed, 26 Jun 1996 13:31:10 -0500
Subject: Re: film resolution

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} } } Frank Scheltens and I recently looked at the new Fuji Imaging plates.
} } } They are spectacular!

The beauty of the Fuji imaging pl
} ates is that their output is linear over about 5 orders of exposure. (Get
} Fuji's literature for all the specific details.
************************

Hi Scott, what bit depth in output precision are provided by the Fugi files
(8-bit, 16-bit)? This is an important measure for the contrast resolution
of the image file. Large exposure range is very nice, but high contrast
resolution is even nicer. Otherwise, your small contrasts will be squished
into only a few intensity seps and get lost, and you will lose much of the
digital advantage.


Best regards Klaus


******************************************************************************
* Klaus-Ruediger Peters, Ph.D. :Molecular Imaging Laboratory *
* Director, Molecular Imaging Laboratgory : http://panda.uchc.edu/ *
* Biomolecular Structure Analysis Center : htklaus/index.html *
* University of Connecticut Health Center : *
* 263 Farmington Ave. :F r e e Access to Differential *
* Farmington, CT 06030-2017; U.S.A :Contrast Software at *
* e-Mail: Peters-at-BSAC.UCHC.EDU : http://panda.uchc.edu/ *
* Tel: (860) 679-3977; Fax: (860) 679-1989 : htklaus/DHP-Img.html#Software*
******************************************************************************







From: chris gilpin :      cgilpin-at-fs1.sem.man.ac.uk
Date: Wed, 26 Jun 1996 11:34:21 BST
Subject: Image archiving software

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Message-Id: {n1376316661.47230-at-ematserv.ruca.ua.ac.be}
"Microscopy-at-Sparc5.Microscopy.Co" {Microscopy-at-Sparc5.Microscopy.Com}
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Reply to: RE} Image archiving software

Hi Chris and all other,

about the archiving software: we use PHRASEA on Macintosh systems. In
principle it should work fine but we've had a lot of troubles with respect to
installation, crashes, lost pictures etc. It looks as if the soft- and
hardware are not as yet fully compatible. We choose this package because it
fitted best with our needs with respect to having a searchable database in a
multiple-user environment.
We plan to store all our images on CD-rom and keep small pictures in the
database.
Also, what do you do with your old images (over 20.000 pictures in our case).
It takes a lot of time to scan them but you need this to have good use for
your look-up tables or searches.
I guess we'll keep working with Phrasea, but I'd like to hear comments on the
practical use of such databases.

Nick Schryvers
Antwerp, Belgium

--------------------------------------

Raining in Manchester!!

Chris

Chris Gilpin
Biological Sciences Electron Microscope Unit
G452 Stopford Building
Oxford Road
Manchester
M13 9PT
phone +44 161 275 5170
fax +44 161 275 5171

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From: Lynn M. Savino :      fams-at-holonet.net
Date: Wed, 26 Jun 1996 15:03:00 +0000
Subject: SCANNING 97

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SCANNING 97
April 19-22
Monterey, California, USA




From: Ann-Fook Yang :      YANGA-at-em.agr.ca
Date: Wed, 26 Jun 1996 15:38:32 -0400
Subject: Immunogold labeling

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X-Mailer: Novell GroupWise 4.1

Recently, someone mentioned that polyclonal antibody has 70%
labeling efficiency while monoclonal antibody has only 5% (I am
not sure about this figure). Have these figures been published?
Where can I find the paper?

Ann Fook Yang





From: owenha-at-wkuvx1.wku.edu (Heather Owen)
Date: Wed, 26 Jun 1996 10:16:04 -0500
Subject: TEM, SEM EDS Positions - W. Ky. U.

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The following two positions are currently available in our department:


ELECTRON MICROSCOPY TECHNICIAN

E M Technician - The Department of Biology at Western Kentucky
University is accepting applications for a full-time, permanent
Electron Microscopy Technician position available immediately. The
major responsibility of this position will be to oversee all activities
associated with the multi-disciplinary user EM Facility. The facility
houses two JEOL 100B TEMs, one JEOL 5400 LV (low vacuum) SEM
with attached KEVEX Sigma Level One EDS, darkroom and full sample
preparation equipment. The technician will be responsible for the
day-to-day operation of the laboratory including routine maintenance
and trouble-shooting, supervision of service personnel, training of
users, and providing consultative and research support for faculty and
students. The qualified candidate should have a B.S. degree (M.S.
preferred) and at least two years of experience, including EDS and
skills in both biological and materials science specimen preparation.
Additional duties (less than 25% time commitment) include
organization of the use of shared equipment in multisection
introductory laboratory courses and maintenance and care of the
departmental greenhouse. Send letter of application, curriculum vitae
and three letters of reference to: Dr. Heather A. Owen, Department of
Biology, Western Kentucky University, Bowling Green, KY
42101-3576. Screening of applications will begin July 1, 1996.
Women and minorities are encouraged to apply. An affirmative action/equal opportunity employer.



POSITION ANNOUNCEMENT
TEMPORARY - 1 YEAR REPLACEMENT

CELL/ULTRASTRUCTURAL BIOLOGIST - The Department of Biology of
Western Kentucky University is accepting applications for a
temporary Assistant Professor position. Responsibilities include cell biology and electron microscopy courses, and supervision of an EM
facility. Research activity is encouraged. Ph.D. or ABD is required. Appointment for fall, 1996. Send application, vitae, statement of
research/teaching interests, and three letters of reference to:
Dr.Blaine Ferrell, Department of Biology, Western Kentucky
University, Bowling Green, KY 42101-3576. Women and minorities
are encouraged to apply. An affirmative action/equal opportunity
employer.


************************************************************
- -
************************************************************
Heather Owen
Department of Biology
Western Kentucky University
1 Big Red Way
Bowling Green, KY 42101-3576

(502) 745-6501 voice, (502) 745-6856 fax, Owenha-at-WKUVX1.WKU.EDU






From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 26 Jun 1996 16:35:35 -0400 (EDT)
Subject: Room-mate(s) for MSA

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I will be attending the MSA meeting, and I want to get inexpensive
lodging. I will be flying in Saturday afternoon or evening and staying
through Thursday. If anyone wishes to split costs by sharing a room, please
let me know. I don't smoke, but I don't mind sharing with someone who does.
TIA.
Yours,
Bill Tivol




From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Wed, 26 Jun 1996 11:50:47 -0400
Subject: WWW Topical Conference in Minneapolis

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Second Reminder, Space Still Available, Please Pre Register.



Here is a reminder and some clarifying information.


The Microbeam Analysis Society is sponsoring their first Topical
Conference immediately prior to the Microscopy and Microanalysis 96
meeting in Minneapolis this year. The Topical Conference is entitled:


{bold} "Microscopy & Microanalysis Resources on The World Wide Web"

(http://www.microanalysis.org/mas/topicalconf96.html

{/bold} http://www.microanalysis.org/mas/regtopconf.html {bold} )

{/bold}

It will be held in The Minneapolis Convention Center on


SATURDAY THE 10TH of AUGUST 1996.


Note that the ad in Microscopy Today contained a typo stating that the
meeting was to be on the Sunday. The meeting has been placed on
SATURDAY to avoid any conflict with the M&M Tutorials.


The starting time will be 9:00am and the conference will last all day.


The morning will be a series of presentations which will cover the
following:


1. Basic introduction to the Web

2. What is available on the Web generally.

3. What is available on the Web for Microscopy & Microanalysis

4. How to create your own Web Page.


Invited Presenters include:

Greg Meeker - U.S. Geological Survey.

Marc De Graef - Carnegie Mellon University.

Darcy Clark - University of Michigan (formerly University of
Queensland).

John Mansfield - University of Michigan.


The afternoon will be focussed on a hands-on workshop where there will
be a minimum of 15 computer workstations connected to the Internet (via
a T1 line)

for attendees to use. The morning's presenters will be available to
advise attendees on all aspects of accessing and using the Web.


Attendance is FREE to MAS members

Attendance is $35 for Non Members

If you join The Microbeam Analysis Society you may attend free,
membership is $25 per year.


You may register by:

1. Filling out the card that was contained in the last issue of
Microscopy Today

and mailing it in to the address noted on the card.

2. You may register electronically if you are a member. Connect to:

http://www.microanalysis.org/mas/regtopconf.html

3. Non members may complete the form at:

http://www.microanalysis.org/mas/regtopconf.html, print it and mail it
to the address supplied in the form.

4. Members & Nonmembers may send their:

Name

Full address

Business phone

FAX

email address

and an indication of their computer and Web expertise to John
Mansfield

at the address at the end of this message.

Non-Members should enclose a check for $35 (drawn on a US bank) made
out to The Microbeam Analysis Society.



For future updates keep monitoring:

http://www.microanalysis.org/mas/topicalconf96.html

or send mail to John Mansfield (jfmjfm-at-umich.edu)


Thanks.




John Mansfield

North Campus Electron Microbeam Analysis Laboratory

417 SRB, University of Michigan

2455 Hayward, Ann Arbor MI 48109-2143

Phone: (313)936-3352 FAX (313)936-3352

Email: jfmjfm-at-engin.umich.edu

URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html





From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 26 Jun 1996 16:41:36 -0500 (EDT)
Subject: Re: TEM biology

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}
} Most labs dehydrate tissue in ethanol when processing for E.M.
}
} I have always used methanol. Is there logic in using one alcohol or the
} other?
}
Dear Sally,
Since water is completely miscible with either alcohol, the only
logical reason I can think of is that methanol is more toxic.
Yours,
Bill Tivol




From: kszaruba-at-MMM.COM (Karen S. Zaruba)
Date: Wed, 26 Jun 1996 17:32:21 -0500
Subject: LM - parts for Zeiss scope (update)

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Message-Id: {199606262227.AA128098060-at-pigseye.mmm.com}
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This is a follow-up to my previous question about sources of used parts for
Zeiss scopes.

First of all, thank you to all who replied!! I got the web address for Zeiss
(http://www.zeiss.com), for Scott Scientific (http://www.scottscientific.com)
as well as suggestions of Spectra Services, Inc. at (716) 654-9500 and West
L.A. Microscope at (800) 794-8898.

The second thing I have to say is Aaaaak! I was afraid this wouldn't be simple.
[I am a newbie to the components of light microscopes, and inherited
this one without an owner's manual]. For those of you who wanted more details,
the scope is a Standard Pol type, with a phototube. The beam stop selects how
much light goes to the phototube (usually either 80% or 100% I think). The 40x
objective says "NeoFluar 40/0.75, Ph2, 160/0.17" and is a Zeiss brand. From
this
I assume it is a Phase 2 and 160 mm (fixed) tubelength.

What I would like is something which gives less blurriness on a relatively
"thick"
section (4-8 um) and does not have Phase, which I don't want to be bothered
with.

Thanks again for the responses and I will check out the above sources!

Karen


Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
3M Company
3M Center 270-1S-01 Phone: 612-737-2971
St. Paul, MN 55144-1000

These opinions are my own and may not represent those of 3M.







From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 26 Jun 1996 12:47:47 -0400 (EDT)
Subject: Re: Catalese problems

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Dear Gib,
} [snip]
} I'll never forget the pleasant shock I got when I found out the effect that
} condenser aperture diameter can have on resolution at the mags you would view
} catalase crystals at. I used to think that the condensor aperture was just a
} coarse brightness control, a big valve on the electron flow. But using a 50
} to 100 micron condenser aperture can show big improvement in resolution over
} a 200 micron size. I've tried to find out why in books on TEM optics theory
} but the turf is fraught with mathematical complexities, contrast transfer
} functions and stuff like that. Can anyone out there give it to me straight?
}
Of course, our geometry is different with the HVEM; we normally use
a 1 mm condenser aperture, but for low-dose and diffraction, we use either
a 100 or 30 micron condenser apertures (unlike nearly everyone else, we often
try to throw away beam current). These smaller apertures select electrons
which are emitted from a smaller area of the (W) filament than does the
large aperture, so the beam is more coherent. The lessening of the overlapped
interference fringes accounts, I think, for the better observed resolution.
The same effect should occur for a LaB6 filament operated in "tip" mode--we
are about to install one, so I'll see for myself soon.

} Of course, objective aperture diameter affects contrast of image too. I
} typically use a 40, sometimes a 20 micron diameter objective aperture for
} viewing bilogical sections. There is probably an optimum combination of the
} two aperture sizes to maximize resolution as a funtion of the other operating
} values, kV, spot size, sample type, etc.

The objective aperture size is simply related to the attainable reso-
lution and related in a more complex way to the contrast. If you look in
diffraction mode, you can see the aperture and the diffracted electrons which
it admits. From the camera length and the size of the image of the aperture,
the resolution can be calculated. The contrast is determined by the electrons
scattered outside the aperture (removed from the image) vs those passed through
the aperture. This ratio depends on the angular distribution of the scattering
(in a crystalline material, whether there are intense, high-order reflections
outside the aperture). As far as optimal combinations is concerned, the main
things to remember are that 1) the size of the finest observed features depends
on the contrast and 2) for biological specimens, only the stain is observed.
Since the stain particles are ~1 nm or so in size, an objective aperture should
be chosen which gives the best combination of beam intensity and contrast, and
this will be specimen-dependent. We normally use a 30 micron aperture, which
nominally gives 0.5 nm resolution. When I was imaging crystals at high reso-
lution (for the HVEM), I used a 50 micron aperture so that the reflections
at 0.3-0.4 nm would contribute to the image. For microtubules, we need a 10
micron aperture to get good contrast, and 1.5 nm is about the size of the
stain, so we don't lose biological info, even though the resolution is "poor".
Yours,
Bill Tivol




From: Probing & Structure :      pns-at-ultra.net.au
Date: Thu, 27 Jun 1996 12:02:45 +1000
Subject: Film resolution

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At 09:08 26-06-96 -0400, Scott D. Walk wrote:
} } If you assume that the eye's resolution is about .1mm (~300dpi), then you
can enlarge a TEM negative (12.5um) by 8x. (From experience, I have printed
TEM negatives on 16 x 20 paper with good results.) The corresponding limit
to enlarging the imaging plates would be 4x.
}
***********************
I am afraid that Scott may have used pixel/area. For resolution
comparisons, one must stay with linear measures. Document films (and that is
what the EM emulsions are) have a resolution of at least 200 lines/mm and
this Kodak film could go to 300 lines/mm. 10 lines/mm resolution for the
unaided eye is a realistic figure and this means that 20 and even 30 times
enlargements will show details not visible at lower enlargements. This is
the theory and I have done that in practice - it works!

The Fuji film comparison Scott made, may!!! be valid, but there are
advantages in having a slower film. As I pointed out in my contribution on
film resolution/ instrument depth-of-field, an image is better when formed
by more electrons (slower film=better). Slower film is also more contrasty
and has better resolution. The slow film's down-side is less exposure
latitude and the faster film excels in that respect and that was Scott's
main observation with the Fuji Film.

Swings and round-abouts, but which is the most suitable film and for which
application?

Jim Darley



Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site http://www.ultra.net.au/~pns/
***********************





From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Wed, 26 Jun 1996 10:05:17 -0500
Subject: Re: film resolution

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} To all:
}
} I was recently asked the following question about film resolution. I have
} seen a discussion of this somewhere but can't seem to locate it now. Can
} anyone point me in the right direction?
}
} Bob Wise
}
} ***************

The comparison of film capture versus digital capture has two important
aspects: spatial resolution and contrast resolution. The spatial resolution
question is already addressed by several responses. We have experience with
the contrast resolution of films since we use differential hysteresis
processing (for free access to the software see below) for contrast
quantitation and contrast imaging of digitized microscopy negatives
(graytone and color).

Film provides up to 14 to 15 bit of contrast resolution using the AGFA
Arcus II scanner ($ 2,000). This is an exceptionally high contrast
resolution and provides astonishing precision and quality of the digitized
information. This means that small contrasts, let's say fine structural
details of fibers of 1-200 intensity steps, have the same range in the
dark areas (intensity range 1-10,000) as well as in the midrange areas
(intensity range 10,000-20,000) and the bright area (intensity range 20,000
- 30,000) of the negative. On negatives digitized at this precision, we
recover information never being expected to exist or been seen before on
the photographic prints, i.e., yesterday, in collaboration with Dr.
Papermaster, University of Texas, San Antonio, we saw in negatives (taken
years ago from in retina section) extracellular fibers of characteristic
structure connecting the cilium with the wall of the periciliar ridge
complex.


Silver halide image processing (conventional photographic techniques) can
not easily access this contrast resolution of the negative. It provides
only local display in a cumbersome fashion (graytone separations procedures
using ion counter diffusion techniques). Thus, using photographic emulsions
as capturing device and a cheep scanner as A/D converter is a good and
practical solution for any imaging lab (only constrained by the limited but
sufficient linearity of the transfer characteristics).

In contrast, high resolution CCD cameras must be cooled for capturing 14 to
15-bit (very expensive) and conventionally they are not linear for their
dose response. Thus the CCD camera data have a very different contrast
resolution behavior in addition to their limited spatial resolution. The
small fiber contrast mentioned above would be reduced in logarithmic
fashion with the intensity of it's background, i.e., already loosing 50% of
it's range in the midtone areas. (Linearization of the signal is easy but
will change the data fidelity).

When dealing with negatives contrast resolution seems to provide a greater
advantage than spatial resolution. And this high contrast resolution is now
accessible through digital contrast imaging. Bye bye photolab!

Best regards Klaus


******************************************************************************
* Klaus-Ruediger Peters, Ph.D. :Molecular Imaging Laboratory *
* Director, Molecular Imaging Laboratgory : http://panda.uchc.edu/ *
* Biomolecular Structure Analysis Center : htklaus/index.html *
* University of Connecticut Health Center : *
* 263 Farmington Ave. :F r e e Access to Differential *
* Farmington, CT 06030-2017; U.S.A :Contrast Software at *
* e-Mail: Peters-at-BSAC.UCHC.EDU : http://panda.uchc.edu/ *
* Tel: (860) 679-3977; Fax: (860) 679-1989 : htklaus/DHP-Img.html#Software*
******************************************************************************







From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Wed, 26 Jun 1996 21:20:37 -0700
Subject: Re: Further Info on Fuji Imaging Plates

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I saw this plate system at a recent meeting, last year at MSA, I think, and
was very intrigued. Although I don't have enough money to buy a system, I
have a few questions about it.

1. Can anyone tell us how many of these units in 3.25 X 4 inch format have
been placed, both worldwide and by region, e.g., US, europe and asia?

2. How are people with these systems archiving data sets of this size, 23MB?


***[snip]***
} The files are 3760 x 3000 x 16-bit (14-bits used) unsigned integer raw
} files (23MB). These can easily be imported into Spyglass,
} DigitalMicrograph, Semper or any other sophisticated image processing
} package that can handle 16-bit data (if you have enough memory!).
***[snip]***

Thanks,

John
chandler-at-lamar.ColoState.EDU






From: Trevor Sewell :      SEWELL-at-uctvms.uct.ac.za
Date: Thu, 27 Jun 1996 08:58:48 +0200
Subject: Re: film resolution

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There seems to be quite a bit of interest in this topic so I have
put a bibliography I compiled last year about imaging technologies
on the web at
http://www.uct.ac.za/depts/emu/imaging/papers.htm

I hope it will be helpful

good papers on film are:
Downing and Grano (1982)
Farnell and Flint (1973) and (1975)
Hahn (1980)
Hamilton and Marchant (1967)

The field was reviewed by Zeitler (1992) Ultramicroscopy 46,405

Trevor Sewell




From: Mark Munro :      m.munro-at-ab.sac.ac.uk
Date: Thu, 27 Jun 1996 08:29:44 0
Subject: Re: image archiving software

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Message-Id: {9606270725.AA26384-at-granite.ab.sac.ac.uk}
Comments: Authenticated sender is {ab157-at-granite.ab.sac.ac.uk}

Dear Chris and all,
We use the Aequitas archiving software from DDL.
We mainly use it to archive light/fluorescence images captured
through a Leica Quantimet 600, but it would be equally applicable to
electron microscopy images. It is a very easy to use windows
program, which allows creation of database forms to the users
specification. It supports full searching of the created databases,
and archiving to whatever media you have available. Please feel free
to contact me if you have any other questions.

Mark Munro.
The Soil Biology Unit
SAC Aberdeen




Mark Munro




From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Thu, 27 Jun 1996 06:39:32 -0500
Subject: Re: Fourier transformation programm

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oconnor-at-ipl.rpi.edu

Addendum: There are some real to complex FFTs in http://risc1.nc1.numis.nwu.edu/fft
which work well - contact me directly if you have problems.




From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Thu, 27 Jun 1996 13:25:36 +0000 (GMT)
Subject: Fuji IP

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On Wed, 26 Jun 1996, Scott D. Walck WL/MLBT wrote:

} Frank Scheltens and I recently looked at the new Fuji Imaging plates.
They are spectacular! The plates are loaded in the same film holders as
regular film. We ran a test where we spread the the beam out until the
lowest current reading on the screen was indicating. Then stepped the
exposure through two plates using an aperture. The exposure times went
from .12 to 600 sec and all the steps were there without saturating the
plates. We did the same test on Kodak SO-163. }

.../...

} Fuji has a program where you can try out their imaging plates if you
are interested. That is how we got them to try out. }
} - -Scott Walck

Just one question. The plate number is usually printed on the
plates using a photonic system, (seems to be a small screen on which data
are printed, and one or two mirrors and a lens allowing to focus the data on
the plate) which works quite well with usual photographic plates.

In the case on Fuji IP, it seems that it does just not work, because IP
are sensitive to electrons and NOT to photons (I may be equivocated but
I do not think so). Do you know if any improvement is in progress? this
could be a new device developed by EM companies or by Fuji itself,
allowing an auxilliary electron beam to print the relevant data on the
plates, or any other system that I have not though about.
It appears that with many users using the same microscope, it is likely
that plates be mixed up, so that just printing numbers on the back of the
plates might be unsufficient.

Another question. What will Fuji price policy be in the future. At the
moment their plates seem to be rather expensive, so that if we want to
equip 5 microscopes with this system we might think twice...

Yet another one. I would be happy to be able to get the plate out of the
microscope just after taking the picture.

I elaborate: with photographic plates I understand that we have to work in
batches because plates are light sensitive. As a consequence one has to
wait till the plate is processed in order to know if it is good or not.
For this reason camera makers do have a great argument telling that the
image can be computer stored instantaneously.

If you can get the IP out of the microscope and have it processed within
minutes this would be a great improvement. It "just" takes a modification
of the photo box, and I imagine that a good engineer could do that. By
solving these two problems I believe that Fuji would clear any arguments
against their system.

Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Luis Sole i Sabaris
E-08028 BARCELONA

Tel +34 3 402 16 95
Fax +34 3 402 13 98





From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 27 Jun 96 09:57:31 EDT
Subject: Zip drives

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This is a question directed to our Friendly Neighborhood SysOp and any vendors
planning to be in Minneapolis 8/10-15. How good are our chances of finding Zip
drive equipped Macintosh computers? It is a wonderful way to transport digital
anything.
Kate Connolly




From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Thu, 27 Jun 1996 11:11:15 -0500
Subject: Re: Fourier transformation programm

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microscopy-at-Sparc5.Microscopy.Com, oconnor-at-ipl.rpi.edu

Sorry, in my last email I used a remote vt100 server which does not
appear to have been setup correctly. The URL is:
http://risc1.numis.nwu.edu/fft




From: Mark Wall :      Mark.Wall-at-quickmail.llnl.gov
Date: 27 Jun 1996 09:37:13 -0700
Subject: Mark Wall

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Message-ID: {n1376254103.20632-at-quickmail.llnl.gov}

Does anyone out there have an old used low speed diamond saw such as those
sold by Buehler, South Bay Technology, etc? Preferably something no longer
needed such that a donation would be in order, else we are willing to purchase
at some reasonable cost. If one is available but needs repair that is O.K.
too. Also looking for an old Fischione electropolishing unit and power supply.

Thanks,
Please respond in private to
Mark Wall, 510 423-7162, USA





From: Raymond F Egerton :      egerton-at-phys.ualberta.ca
Date: Thu, 27 Jun 1996 08:01:16 -0600 (MDT)
Subject: RE: Fourier transformation programm (fwd)

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Dear Wei
I can help you with your Image plate RDF problem.
1. To analyse the data, you should use the method of Cockayne et al. See Acta
Cryst. A44, p.870 (1988). and earlier papers. This is important.
2. To convert the IP data to intensity, you need the conversion table and the
sensitivity setting used to read the data. This is available from our computer
specialist, Paul Perkes. (We also have a Fuji Image plate reader). You can
contact him using the address paulperkes-at-asu.edu
Regards, John Spence




From: mrdunlap-at-ucdavis.edu (Michael Dunlap)
Date: Thu, 27 Jun 1996 08:53:46 -0700
Subject: Quantamet 900

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We are clearing space and would like to get rid of an old Quantamet 900.
We asking a nominal fee. Sorry, I wanted to give it away or dump it. If
you are intrested in it please contact me and I will get back to you.

Mike

===========================================================
Michael Dunlap lab (916) 752-0284
Facility For Advanced Instrumentation fax (510) 422-2282
University of California mrdunlap-at-ucdavis.edu
Davis CA, 95616
http://carbon.ucdavis.edu
============================================================






From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Thu, 27 Jun 1996 16:04:54 -0400
Subject: Re: Zip drives

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} This is a question directed to our Friendly Neighborhood SysOp and any
} vendors
} planning to be in Minneapolis 8/10-15. How good are our chances of finding Zip
} drive equipped Macintosh computers? It is a wonderful way to transport digital
} anything.
} Kate Connolly

There will be at least one ZIP drive on a Mac and one on a PC at the
Computer Workshop at the M&M96 meeting.

Jfm.


John Mansfield
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html






From: kaurin-at-rmslab.rockefeller.edu
Date: Thu, 27 Jun 1996 12:34:00 EST
Subject: POSITION AVAILABLE--EM TECH OR POST-DOC

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A permanent (at least 4 years) position will be available late summer/
early fall at The Rockefeller University/Cornell Medical School in one of the
nicest areas of New York City.

I am looking for an experienced EM technician or posdoctoral fellow
well-trained in EM techniques to carry on an exciting project involving post-
embedding immunoelectron microscopy. We are studying the distribution of
plasma membrane cell adhesion molecules during the inflammatory response, as
well as changes in cell-cell interactions at the ultrastructural level. The
study is funded by a major NIH grant. It is one aspect of a multidisciplinary
approach to cell adhesion molecules that my lab is taking. All of the investi-
gators interact extensively on the scientific level.

This person would be involved in and responsible for all aspects of this
project including processing of specimens, fixation, embedding, sectioning,
examination, photography, and data collection and analysis. The successful ap-
plicant should have at least a B.A. degree and several years of intensive
experience in electron microscropic techniques. Experience with postembedding
immunoEM is highly desirable, but not necessary. ImmunoEM (by transmission
electron microscopy) is the method we are presently using. In the future we
may contemplate SEM approaches, as well. Competitive salary, commensurate with
experience.

Please send resume to:
William A. Muller, MD, PhD
Associate Professor
Laboratory of Cellular Physiology and Immunology
The Rockefeller University
1230 York Avenue
New York, NY 10021

fax [preferred method of communication] (212) 327-8875

e-mail: mullerw-at-rmslab.rockefeller.edu
(Please DO NOT reply directly to KAURIN-at-rmslab.rockefeller.edu)

I look forward to hearing from you.
--Dr. William A. Muller




From: bozzola-at-siu.edu (John J. Bozzola)
Date: Thu, 27 Jun 1996 18:14:32 -0600
Subject: All: Counting & Marking Pen

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
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Priority: normal
Sensitivity: Company-Confidential

We are trying to locate a marker pen that will both count an object when
touched and mark it so that it will not be recounted. Does anyone know
where we could purchase such a device? Thanks.

####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Thu, 27 Jun 1996 21:33:11 -0800
Subject: Re: SEM/EMPA: carbon evaporation questions

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Dear John,
I have used both carbon and graphite rods in my evapoator, and I think
either will work. The graphite is more conductive, so you must use a higher
current to get it to evaporate, but it is stronger and less likely to break
off. I personally prefer the pressed carbon. In answer to your second
question, I've found that if you can carefully tighten the rod in the
holder without applying sideways stress to it, it is less likely to break.
In my system that means tightening up the screws while holding the
sharpened rod off the flat piece, then carefully letting it come to rest on
the middle of the flat piece.
John wrote:
} I have two questions regarding carbon evaporation (for applying a conductive
} coat to non-conductive materials):
}
} 1. Is there any difference between using graphite vs carbon rods?
}
} 2. I use 3 mm diameter rods, with one rod flat and the other sharpened to
} a 1 mm tip about 3 mm long. Many times the small tip breaks off during
} heating, requiring running the samples over. Any ideas on how to reduce the
} breaking of the rods?
Luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Gordon Watt :      gwatt-at-brookes.ac.uk
Date: Fri, 28 Jun 1996 10:59:10 -0700
Subject: SEM: Etching silicon using Wright solution

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Message-ID: {31D41D6E.7AC2-at-brookes.ac.uk}

I recently read an article by Sekiguchi and Sumino (ref. below) in which they etch silicon with "Sirtl or
Wright" solution. This facilitates observation of dislocations using SEM.

I'd be really grateful if anybody could tell me:

(i) What this solution is?
(ii) How it's used (references?)
(iii) Whether it would work on silica (quartz)?

Thanks in advance

Gordon



Sekiguchi, T. & Sumino, K., 1996. Cathodoluminescence study on dislocations in silicon. J. Applied Phys., 79,
3253-3260


****************************************************************************
Dr Gordon R. Watt,
Geology & Cartography Division,
Oxford Brookes University,
Headington,
Oxford,
OX3 0BP

Tel: 01865 483603
Fax: 01865 483694
e-mail: gwatt-at-brookes.ac.uk
****************************************************************************




From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Fri, 28 Jun 1996 10:57:16 +0000
Subject: Re: SEM/EMPA: carbon evaporation questions

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} I have two questions regarding carbon evaporation (for applying a conductive
} coat to non-conductive materials):
}
} 1. Is there any difference between using graphite vs carbon rods?
}
} 2. I use 3 mm diameter rods, with one rod flat and the other sharpened to
} a 1 mm tip about 3 mm long. Many times the small tip breaks off during
} heating, requiring running the samples over. Any ideas on how to reduce the
} breaking of the rods?

John,

I think that you will find that a number of suppliers of carbon evaporation
equipment now recommend carbon 'string', in preference to carbon rods. This
'string' is a multi filament braid and avoids precisely the type of problem
you mention. Additionally, I think you will find that using 'string' the
whole evaporation process is rather more controllable - does anyone know of
any disadvantage of carbon string (apart from requiring a different
evaporator head)?

Reagards,
Larry Stoter






From: rnessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Fri, 28 Jun 1996 08:01:15 -0500 (CDT)
Subject: Where does the nucleus go?

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Leave it to a young mind to pose an unusual question. We do many tours
of our facility to lots of different groups. Yesterday, we had a group of
gifted high school students come through. While in the tour host mode, I was
explaining thermionic emission at the TEM. One student asked the question, what
happens to the tungsten nucleus after the electrons are freed and removed? I
was honest, and said that I had never really thought about it. I mentioned that
I do clean some contaminants from the wehnelt and anode when I change the
filament, and this could be possible where the protons and neutrons go. Do the
nuclei migrate towards tungsten atoms with more electrons, trying to steal electrons
from those richer in them? Inquiring minds want to know....

Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu





From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Fri, 28 Jun 1996 09:31:58 -0500
Subject: Re: All: Counting & Marking Pen

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Message-Id: {199606281427.JAA23138-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
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} We are trying to locate a marker pen that will both count an object when
} touched and mark it so that it will not be recounted. Does anyone know
} where we could purchase such a device? Thanks.
}
} ####################################################################
} John J. Bozzola, Ph.D., Director

John,
95/96 Fisher catalog, pg 1178, cat# 07-910-15, $175, made by Manostat.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********






From: rh208-at-cus.cam.ac.uk (Ray Hicks)
Date: Fri, 28 Jun 1996 14:52:06 +0100
Subject: Re: All: Counting & Marking Pen

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Hi again John,

As with the other thing, would you keep me posted, this time if you don't
get a useful answer I should be able to build one for next to nothing.

Alternatively, if you can put an image of the objects into a macintosh,
there is a great freeware image analysis program (NIH-Image, available via
ftp from zippy.nimh.nih.gov/pub/mac/nih-image - check the directory tree
yourself) that allows you to do the same thing with a mouse, ie you click
the cursor on the object and the program records the x/y coordinates of the
object and marks it with a dot. The program also has many automatic
dot/particle analysis features, and some handy enhancement routines.

Ray

At 6:14 pm 27/6/96, John J. Bozzola wrote:
} We are trying to locate a marker pen that will both count an object when
} touched and mark it so that it will not be recounted. Does anyone know
} where we could purchase such a device? Thanks.
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Center for Electron Microscopy
} Southern Illinois University
} Carbondale, IL 62901-4402
} U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################

Ray Hicks
________________________________________________________________________
|University of Cambridge |Tel 01223 330149 |
|Department of Medicine |Fax 01223 336846 |
|Level 5, Addenbrookes Hospital |e-mail rh208-at-cus.cam.ac.uk |
|Hills Road Cambridge |Web Page/ facsmac.med.cam.ac.uk |
|CB2 |ftp server 131.111.80.78 |
|UK | |
|_________________________________|_____________________________________|






From: Philip Koeck :      Philip.Koeck-at-csb.ki.se
Date: Fri, 28 Jun 1996 17:34:23 +0200
Subject: digitization

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Posted-Date: Fri, 28 Jun 1996 17:34:23 +0200
Message-Id: {31D3FB7F.27A6-at-csb.ki.se}

I'm looking for some theory (or experience) on the effect of binning on image quality.
Given an image with a certain noise level, how fine does the binning need to be to get the full information
content (especially if I'm going to average the digitized images to reduce noise)?

Thank You in advance

Philip
--
Philip Koeck
Karolinska Institutet
Dept. of Bioscience
Novum
S-14157 Huddinge
Sweden
Tel.: +46-8-608 91 93
Fax.: +46-8-608 92 90
Email: Philip.Koeck-at-csb.ki.se




From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Fri, 28 Jun 1996 12:28:13 -0400
Subject: TEM instrumentation - a question

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Here is a question that I received and would like to forward to the
list. Please send the replies directly to " rv-at-swifty.pse.umass.edu" as they
do not subscribe to the list. Thanks


} X-POP3-Rcpt: sdw-at-snitch
} Date: Wed, 26 Jun 1996 14:10:37 -0400
} From: Regina Valluzzi {rv-at-squeaky.pse.umass.edu}
} Subject: TEM instrumentation - a question
} Sender: rv-at-swifty.pse.umass.edu
} To: sdw-at-biotech.ufl.edu
} Organization: UMass Polymer Science
} X-Url: http://www.biotech.ufl.edu/~emcl/tips.html
}
} I noticed that the types and tricks seem to be answers to peoples
} questions, and was wondering if anyone out there had any ideas about
} something our 200 kV TEM has been doing. We have a tungsten filament in
} our microscope, and I've noticed that there are 2 intensity maxima when
} I slowly saturate the filament. If I go to crossover and slowly
} desaturate the filament, the tip(s) can be imaged at both maxima and the
} images are different, but both look filament tip-like. The beam current
} also seems really high. The two maxima occurred with a previous
} filament and the beam current was also really high (at the second
} intensity maximum, the first maximum is at the current I'd expect from
} prior experience). Our technician doesn't seem to find this unusual, so
} perhaps it's not a problem, but we go through many filaments in a
} typical year. Anyone know if this is a problem? Anyone know how to fix
} it ?
}
}




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 28 Jun 1996 14:34:52 -0400
Subject: RE- where nuclei go

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Message-ID: {n1376149900.49763-at-mse.engin.umich.edu}

Subject: Time: 2:27 PM
OFFICE MEMO RE: where nuclei go Date: 6/28/96

Apart from those that evaporate from the filament, or those that move around
within the body of the filament itself, due to thermal effects, I don't think
much of anything happens to the nuclei. In metals, the valence electrons
reside in a valence "band", meaning that their energy states are related to
the overall structurel arrangement of the atoms in the crystal (rather than
being determined by the nearby atoms to which they are bonded, as in organic
molecules where we have localized covlaent bonds, and in ionic compounds
where we have well defined ions). In this situation, the electrons move
rather independently of the individual nuclei (that is 'they belong to the
overall crystal' rather than to individual nuclei) and so the electrons that
are emitted from the filament are simply replaced by ones that are fed into
it from the external the circuit running from your local power company, and
probably the nuclei don't know or care very much about the fact that this
process is going on around them. For a discussion of metallic bonding, see
the chapter on Atomic Structure and Interatomic Bonding in the book
"Materials Science & Engineering" by W. D. Callister (in the 2nd Edition,
this was Ch. 2 - I don't know about more recent editions).





From: Paul Krumpe :      pkrumpe-at-iplab.com
Date: Fri, 28 Jun 1996 11:2:33 -400
Subject: Re: Image archiving software

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Message-Id: {199606281930.PAA19185-at-andrew.cais.com}

Reply to: RE} Image archiving software
RE} Image archiving software:

Our company, Signal Analytics, has a new Image Cataloger module, an add-on
to our main product, IPLab Spectrum image analysis software (currently
for Macintosh). With this module, IPLab Spectrum offers integrated image
acquisition, processing, and archiving.

If anyone would like further information, please contact us at
{info-at-iplab.com} .


Paul Krumpe
pkrumpe-at-iplab.com
--------------------------------------





From: rybicka-at-acsu.buffalo.edu
Date: Fri, 28 Jun 1996 15:47:31 -0400 (EDT)
Subject: glycogen revisited

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Dear Friends,
I have just published a review article entitled "Glycosomes - the
organelles of glycogen metabolism" in Tissue & Cell 28 (3) 253-265,1996
which is a revision of the EM interpretation of glycogen. The review is
based on biochemical and microscopical studies, includes the history of
glycogen research in each discipline as well as the current status of
knowledge.
The conclusion I have reached from my studies is that glycogen
occurs in the cell as part of organelles, called glycosomes, composed of
glycogen and the enzymes involved in its metabolism. The 20-30 nm granules
visible in sections stained with uranyl and lead and commonly interpreted
as particles of glycogen, represent only the protein component of
glycosomes. I feel that the revision of this point is of particular
importance in EM research. It may seem like a small point, for there is
no doubt that glycogen is associated with glycosomal protein, however,
the size and the electron density of protein prticle indicates the
metabolic state of the organelle rather then the amount of glycogen.
The recognition and understanding of the nature of glycosomes opens
a wide field for microscopical research on glycosomal enzymes, on the
association of glycosomes with other cellular organelles and the role
played by glycogen in the regulation of physiological processes.
I would be very happy to receive some feedback on the ideas I
outlined in my review article and to continue discussion with anyone
interested in glycogen research.

Krystyna Kielan Rybicka




|--
-----------------------------------------------------------------------------
name: Krystyna Rybicka
email address: rybicka-at-acsu.buffalo.edu
lab phone # : 716-829-3575
-----------------------------------------------------------------------------






From: fams-at-holonet.net (Scanning)
Date: Fri, 28 Jun 1996 07:24:39 -0700
Subject: SCANNING 97

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From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 28 Jun 1996 16:04:21 -0400 (EDT)
Subject: Re: Fuji IP

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} Just one question. The plate number is usually printed on the
} plates using a photonic system, (seems to be a small screen on which data
} are printed, and one or two mirrors and a lens allowing to focus the data on
} the plate) which works quite well with usual photographic plates.
}
} In the case on Fuji IP, it seems that it does just not work, because IP
} are sensitive to electrons and NOT to photons (I may be equivocated but
} I do not think so).

It is my understanding that IP's *are* sensitive to photons (at least
in some frequency ranges). Fuji would know whether the usual illumination
system would work. We have a xenon flash tube and glass prisms/lenses, so
maybe there are enough high-frequency photons.

} Do you know if any improvement is in progress? this
} could be a new device developed by EM companies or by Fuji itself,
} allowing an auxilliary electron beam to print the relevant data on the
} plates, or any other system that I have not though about.

It would seem that a CRT-type beam could be used to write this way.

} Another question. What will Fuji price policy be in the future. At the
} moment their plates seem to be rather expensive, so that if we want to
} equip 5 microscopes with this system we might think twice...
}
Since the plates are reusable, it is only a one-time expense.

} Yet another one. I would be happy to be able to get the plate out of the
} microscope just after taking the picture.
}
} I elaborate: with photographic plates I understand that we have to work in
} batches because plates are light sensitive.

IP's are also light-sensitive--you should not expose them to fluor-
escent light when taking them to the reader.

} As a consequence one has to
} wait till the plate is processed in order to know if it is good or not.
} For this reason camera makers do have a great argument telling that the
} image can be computer stored instantaneously.
}
} If you can get the IP out of the microscope and have it processed within
} minutes this would be a great improvement. It "just" takes a modification
} of the photo box, and I imagine that a good engineer could do that. By
} solving these two problems I believe that Fuji would clear any arguments
} against their system.
}
This is as easy as removing a single piece of film--no problem on
our scope. The readers I've seen take on the order of a few minutes to
process an IP.
Yours,
Bill Tivol




From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 28 Jun 1996 16:36:26 -0400 (EDT)
Subject: Re: Where does the nucleus go?

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} One student asked the question, what
} happens to the tungsten nucleus after the electrons are freed and removed? I
} was honest, and said that I had never really thought about it. I mentioned
} that I do clean some contaminants from the wehnelt and anode when I change
} the filament, and this could be possible where the protons and neutrons go.
} Do the nuclei migrate towards tungsten atoms with more electrons, trying to
} steal electrons from those richer in them? Inquiring minds want to know....
}
Dear Randy,
First, only a few of the electrons are removed from the tungsten, and
since it is metallic, these electrons are in the valence band associated with
the entire crystal (or the surface, anyway) rather than any particular atom.
Second, the thermionic process which removes the electrons occurs at a temp-
erture of a few kK--equivalent to ~1 eV--which is not enough to displace
nuclei; however, at that temperature atoms can get sufficient energy to leave
the surface. That is, the tungsten will sublime. The tungsten vapor then
condenses on the wehnelts, anode, etc., causing the contamination. Third,
unless you have hydrogen in your tungsten, there will be no free protons, and
even at our high voltage (1.2 MV) there is insufficient energy to produce
free neutrons (and, of course, the HV is not at the filament). Tungsten atoms
(not nuclei) do migrate, as the field ion microscope demonstrates, but it is
not as if they were bare nuclei drawn toward electron-rich atoms. I hope
this satisfies the inquiring minds.
Yours,
Bill Tivol




From: Gordon Watt :      gwatt-at-brookes.ac.uk
Date: Fri, 28 Jun 1996 10:59:10 -0700
Subject: SEM: Etching silicon using Wright solution

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"microscopy mailing list" {Microscopy-at-Sparc5.Microscopy.Com}
Cc: "Watt, Gordon" {gwatt-at-brookes.ac.uk}
X-Mailer: Mail*Link SMTP-QM 3.0.2 GM

RE} SEM: Etching silicon using WrightÉ 6/28/96

We don't use Sirtl anymore, however, here is the story on it. A paper was
presented in 1961 by Von Erhard Sirtl and Annemarie Adler of Siemens in
Munich, Germany. They described an etch which decorates defects such as
dislocations and stacking faults in silicon. The mix specified in the paper
was 46gms CrO3 in 100gms of 40% HF; and was reported to be stable with use,
and useful over a wide range of concentrations. It cannot be stored in
bottles such as Nalgene because the bottles will slowly decompose, turn green,
and become brittle enough to break. ( When we used it, we would always create
a new solution each day).
Wright etch is a defect etch for silicon and is an improved version of
Sirtl etch. Wright etch was developed by Margaret Wright Jenkins of Motorola.
The Wright etch consists of a mixture of 30ml of 5 molal chromic (CrO3), 60ml
hydrofluoric (HF), and 30ml nitric (HNO3), buffered with 60ml glacial acetic
acid and 60ml water. Two grams of copper nitrate (Cu(NO3)2) or copper sulfate
(CuSO4) are also used to decorate the junction. Wright etch decorates defects
on both {100} and {111} planes in n- and p-type material, over a wide range of
resistivities. Wright etch preferentially delineates defects resulting from
high-temperature processing steps.
The reference paper for the etch is:
"A New Preferential Etch for In Silicon Crystals" by Margaret Wright
Jenkins, Journal of the Electrochemical Society, 124, 1977, p757.
The etch is available from Olin Corp. by the name "Wright Etch X-17"


**********************************************************
Jake Schaper
Product Analysis Lab
Application Specific Integrated Circuit Division
Motorola, Inc.
1300 N. Alma School Rd. Chandler, Arizona 85224
Mail Drop CH240
Phone 602-814-4756
**********************************************************


--------------------------------------

I'd be really grateful if anybody could tell me:

(i) What this solution is?
(ii) How it's used (references?)
(iii) Whether it would work on silica (quartz)?

Thanks in advance

Gordon



Sekiguchi, T. & Sumino, K., 1996. Cathodoluminescence study on dislocations in
silicon. J. Applied Phys., 79,
3253-3260


****************************************************************************
Dr Gordon R. Watt,
Geology & Cartography Division,
Oxford Brookes University,
Headington,
Oxford,
OX3 0BP

Tel: 01865 483603
Fax: 01865 483694
e-mail: gwatt-at-brookes.ac.uk
****************************************************************************

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From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Fri, 28 Jun 1996 12:28:13 -0400
Subject: TEM instrumentation - a question

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Message-ID: {n1376146995.23161-at-mse.engin.umich.edu}
"Scott Whittaker" {sdw-at-biotech.ufl.edu}
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Reply to: RE} TEM instrumentation - a question

When a filament is heated to the point that "saturation" occurs, what is
actually happening in the electron gun is that the emission current through
the filament is increased. This current flows through a bias resistor, and
this causes the bias voltage between the filament and the grid cap to
increase to such a level that the equipotential surfaces around the filament
tip attain sufficient curvature to cause the electron beam to be focused to
aspot somewhere in front of the grid cap. This process was first fully
understood by Haine & Epstein in 1952, and is nicely described in Haine's
book, "The Electron Microscope" which was published by Interscience in the
mid 1950's (Chs. VI and VII). I do not know the exact characteristics of
your electron gun, which depend on the size and shape of the hole in the grid
cap and the position of the filament relative to that hole, but is sounds as
though you have a situation where you are able to take the process through
two successive focusing conditions. It is quite possible to do this in
electron lenses - we once had a microprobe that had such a strong power
supply for the condenser lens that we could run the beam through two
successive focus conditions if we ran the power to the lens through the whole
range provided by the power supply. While this was an electromagnetic lens,
and you are dealing with a very primative electrostatic lens, it nonetheless
sounds like what is happening in your gun.
In any event, filament life is exponentially dependent on filament
temperature. So, to increase filament life you should certainly operate at
the the crossover or saturation condition corresponding to the lowest
filament heating current.

--------------------------------------

Here is a question that I received and would like to forward to the
list. Please send the replies directly to " rv-at-swifty.pse.umass.edu" as they
do not subscribe to the list. Thanks


} X-POP3-Rcpt: sdw-at-snitch
} Date: Wed, 26 Jun 1996 14:10:37 -0400
} From: Regina Valluzzi {rv-at-squeaky.pse.umass.edu}
} Subject: TEM instrumentation - a question
} Sender: rv-at-swifty.pse.umass.edu
} To: sdw-at-biotech.ufl.edu
} Organization: UMass Polymer Science
} X-Url: http://www.biotech.ufl.edu/~emcl/tips.html
}
} I noticed that the types and tricks seem to be answers to peoples
} questions, and was wondering if anyone out there had any ideas about
} something our 200 kV TEM has been doing. We have a tungsten filament in
} our microscope, and I've noticed that there are 2 intensity maxima when
} I slowly saturate the filament. If I go to crossover and slowly
} desaturate the filament, the tip(s) can be imaged at both maxima and the
} images are different, but both look filament tip-like. The beam current
} also seems really high. The two maxima occurred with a previous
} filament and the beam current was also really high (at the second
} intensity maximum, the first maximum is at the current I'd expect from
} prior experience). Our technician doesn't seem to find this unusual, so
} perhaps it's not a problem, but we go through many filaments in a
} typical year. Anyone know if this is a problem? Anyone know how to fix
} it ?
}
}




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/


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From: Mike McKim :      mkm-at-cpcnet.com
Date: Fri, 28 Jun 1996 21:53:04 -0400
Subject: nuclei electron replacement

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The electrons leave but are replaced by electrons supplied by the cathode
potential applied to the filament.





From: Don Chernoff at ASM :      asm-at-indy.net (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Fri, 28 Jun 1996 21:27:38 -0500
Subject: Sending Email to the Microscopy Listserver as an Attachment

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Subscribers...

Don Chernoff pointed out to me that some people are
begining to send attachments to Email to the listserver.
After giving the issue due consideration I have come
to the conclusion that this method is not in the best interest
of ALL the people on the server. Please refrain from attaching
any document to Email addressed to this system. Remember
not all attachments are created equal, and just because your
particuliar flavor of software can handle the job, another person's
might not. This may be particuliarly true for many of our subscribers
who access this system by conventional ASCII text mailers.

I have amended the Listserver Instructions to indicate these
instructions, but given the number of people that do not subscribe/
unsubscribe correctly I wonder how many will read that far.

Nestor
Your Friendly Neighborhood SysOp

-----------------------------------
Addition to the Listserver Instructions
-----------------------------------

Can I send an Email Attachment?
------------------------------

Please DO NOT send files as an attachment to the Listserver.

Many people use e-mail software which displays plain text messages
automatically but requires several manual steps to read and delete
or save
an attachment Others have no capabilities at all for extracting and
or translating attachments. Please send all messages as plain text.
This will insure that ALL subscribers to the Listserver can rea ALL
messages...

===========================================================================







From: Emeylan-at-aol.com
Date: Sat, 29 Jun 1996 10:04:42 -0400
Subject: Re: LM - Need parts for Zeiss scope

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Dear Karen,

I service and sell microscopes. Mostly used and 90% Carl Zeiss.

I do have some accessories, and if I do not have what you need, I can find
it.

Please reply to:

Emile Meylan
76227.776-at-compuserve.com

SERCO Technical Services, Inc.
1069 Norfolk Rd.
Livermore, CA 94550

tel: 800.483.0508
fax: 510.443.2049




From: em-at-mediacity.com (Ed Monberg)
Date: Sat, 29 Jun 1996 17:14:47 -0800
Subject: Re: nuclei electron replacement

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} The electrons leave but are replaced by electrons supplied by the cathode
} potential applied to the filament.



True, but only to the extent that each individual e- requires a nudge of,

just to estimate, less than volts, less than milli-volts, less than micro-volts,

less than nano-volts, and more in the range of pico-volts, per electron.

To be more descriptive however, thermal forces stir up

the collection of e-'s in the entire conducting part into a cloud.

By far, most of the cathode potential serves to accelerate the e-'s as they

accelerate after leaving the cathode. The loss of each individual electron

is shared among ". . billions and billions . ." of neighboring atoms

(say, 10e9 per cubic micron, or 10e18 per cubic millimeter)



Several excellent answers have been given already, but it important to
note that atoms are VERY small, and that the quantum physics leads to
interesting conclusions.


The question which stimulated all these replies is a fundamentally
excellent one to stir our wonder and was not in any way answered
well until the advent of quantum physics in the twentieth century,
after the photovoltaic effect was noted, where e- emission is related
to the color of the light shining on the surface of the metal.








Regards,



(signed) Ed Monberg {em-at-mediacity.com}

--------------------------------------------------

510-429-1060 Fax 429-1065
LMDC, (Laser & Motion Development Co.)
3101 Whipple Road
Union City, CA 94587-1216


For our Most recent Catalogue of "On Hand" EQUIPMENT:
Send empty mail to: {Cat-at-lasermotion.com}


Our web page: http://www.lasermotion.com (Is beginning to take shape!)
Our e-mail: office-at-lasermotion.com

{----------------------------- Our page width --------------------------}






From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Sun, 30 Jun 1996 13:25:54 +0000 (GMT)
Subject: Re: Fuji IP

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Bill, thank you for your reply. Yet despite I believe IP is a rather nice
system there are some points remaining unclear for me. Maybe I am asking
too much...

On Fri, 28 Jun 1996, William Tivol wrote:


} } Another question. What will Fuji price policy be in the future. At the
} } moment their plates seem to be rather expensive, so that if we want to
} } equip 5 microscopes with this system we might think twice...
} }
} Since the plates are reusable, it is only a one-time expense.

this is not perfectly true. Fuji claims their plates are reusable 500
times, so you may have to buy new ones every few years. It comes to be
a bit less expensive than photo plates, but you need to add the reader's
price. Therefore it is more expensive actually.

} } Yet another one. I would be happy to be able to get the plate out of the
} } microscope just after taking the picture.
} } I elaborate: with photographic plates I understand that we have to work in
} } batches because plates are light sensitive.
}
} IP's are also light-sensitive--you should not expose them to fluor-
} escent light when taking them to the reader.

One of the argument of Fuji is precisely that dark room is not necessary
any more. So here is something that I do not understand clearly. On the
other hand I repeat that I have never seen any number printed on any IP,
therefore it must be quite less sensitive than photo plates.

} } As a consequence one has to
} } wait till the plate is processed in order to know if it is good or not.
} } For this reason camera makers do have a great argument telling that the
} } image can be computer stored instantaneously.
} }
} } If you can get the IP out of the microscope and have it processed within
} } minutes this would be a great improvement. It "just" takes a modification
} } of the photo box, and I imagine that a good engineer could do that. By
} } solving these two problems I believe that Fuji would clear any arguments
} } against their system.
} }
} This is as easy as removing a single piece of film--no problem on
} our scope. The readers I've seen take on the order of a few minutes to
} process an IP.

Right. But if you make high resolution images, you may want to know
quickly if the photo was successful or not (focus, drift). Therefore being
able to see the result just after taking the picture can be a real
improvement versus normal photo plates. On a Philips microscope, removing
one plate is the same as removing 36: you HAVE to switch off the HT. this
means that when working in high resolution it is not so easy. The system
I imagine would allow you to get the plate out the microscope within
seconds, without generating any perturbation in the vacuum system.



Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Luis Sole i Sabaris
E-08028 BARCELONA

Tel +34 3 402 16 95
Fax +34 3 402 13 98





From: zaluzec-at-sparc5.microscopy.com (Nestor J. Zaluzec)
Date: Sun, 30 Jun 1996 14:54:52 -0500
Subject: software for automated detection of gold particles on EM photos

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} We are looking at large numbers of electron micrographs on which we want to
} quantitate the number of gold particles. Does anyone know of any program
} that can take an electron micrograph (or a scanned TIFF image of a
} micrograph) and count the number of gold particles? If there is such a
} program, can it be used to count gold particles of two different sizes?
}


NIH Image (Mac Version) by Wayne Rasband would be IMHO ideal for you. It
will count the particles
in a field of view as well give you their relative sizes (areas).
Documentation is
excellent and also available. NIH Image is public domain and free.

You can download by FTP a copy from the following sites:

zippy.nimh.nih.gov (site maintained by NIH & best place to look
for current info)
www.amc.anl.gov (site maintained by Nestor for Microscopy &
Microanalysis
usually a version behind
but accessible)


log into either specifying a user name of

anonymous

and a password of

youremailaddress-at-yourhostcomputerdomain

at either site you will have to search the directory structure to find the
right place but it will be obvious.

on Zippy look under /pub/nih-image

on Nestor look under
/ANLSoftwareLibrary/4-MacShareware/Imaging/NIH-Image & SpinOffs


The current version is 1.60.

Nestor
Your Friendly Neighborhood SysOp






From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Mon, 01 Jul 1996 10:04:42 -0500
Subject: Re: digitization

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Would you not have to set the size of the bins to be finer than the
magnitude of the noise in your signal in order to benefit from the image
averaging? Our SEM images are collected at 256 gray levels and the noise is
typically several gray levels in amplitude. I think you would want to do the
averaging before the thresholding or segmentation or binning. Otherwise you
would be "taking a vote" as to whether a given pixel should be of this level
or that. Maybe that will produce the same result mathematically, but it
seems counter-intuitive. You would still need to accurately set you bin
boundaries (or thresholds) to properly split the gray level contrast.

Unless you are really hard pressed for disk or RAM, I would think it easier
just to leave the image at full gray level resolution until the very end. I
think most commercial image processors represent an image using 256 gray
levels internally, even if they contain less than 256 gray levels. So you
really wouldn't be helping yourself out much. You could benefit from using
image compression when storing your files. But then, you would have to spend
some CPU time to uncompress the files, but that should be quite fast except
on the slowest processors. But it will be harder to visually judge the
imporvement if you bin the images early on.

I would leave the images at 256 gray levels until the end if possible.

At 11:52 AM 7/1/96 +0200, you wrote:
} a recipient wrote:
} }
} } What is binning?
}
}
} I'm sorry, I might have misused a term reserved for histograms.
} By binning I mean assigning an integer to a certain range of grey levels
} on the micrograph. Everything from black to dark grey would be represented
} as 0 in the digital image, dark grey to middle grey as 1 etc.. The finer
} the binning the more numbers (bins) you need to represent all the grey
} levels present on the micrograph.
}
} My original question was:
}
} } I'm looking for some theory (or experience) on the effect of binning on
} } image quality. Given an image with a certain noise level, how fine does
} } the binning need to be to get the full information content?
} } (especially if I'm going to average the digitized images to reduce
} } noise)
}
} Philip
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Philip Koeck :      Philip.Koeck-at-csb.ki.se
Date: Mon, 01 Jul 1996 11:52:26 +0200
Subject: Re: digitization

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Posted-Date: Mon, 01 Jul 1996 11:52:26 +0200
Message-Id: {31D79FDA.119F-at-csb.ki.se}

a recipient wrote:
}
} What is binning?


I'm sorry, I might have misused a term reserved for histograms.
By binning I mean assigning an integer to a certain range of grey levels
on the micrograph. Everything from black to dark grey would be represented
as 0 in the digital image, dark grey to middle grey as 1 etc.. The finer
the binning the more numbers (bins) you need to represent all the grey
levels present on the micrograph.

My original question was:

} I'm looking for some theory (or experience) on the effect of binning on
} image quality. Given an image with a certain noise level, how fine does
} the binning need to be to get the full information content?
} (especially if I'm going to average the digitized images to reduce
} noise)

Philip

--
Philip Koeck
Karolinska Institutet
Dept. of Bioscience
Novum
S-14157 Huddinge
Sweden
Tel.: +46-8-608 91 93
Fax.: +46-8-608 92 90
Email: Philip.Koeck-at-csb.ki.se




From: Sanford Simon :      simon-at-rockvax.rockefeller.edu
Date: Sun, 30 Jun 1996 14:50:10 -0400
Subject: automated detection of gold particles on EM photos

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We are looking at large numbers of electron micrographs on which we want to
quantitate the number of gold particles. Does anyone know of any program
that can take an electron micrograph (or a scanned TIFF image of a
micrograph) and count the number of gold particles? If there is such a
program, can it be used to count gold particles of two different sizes?

Thanks,
Sanford Simon
Sanford M. Simon
Laboratory of Cellular Biophysics
Box 304
Rockefeller University
1230 York Avenue
New York, N.Y. 10021
212-327-8130 (voice)
212-327-8022 (fax)
simon-at-rockvax.rockefeller.edu (e-mail)





From: Murphy, Judy :      murphy-at-sjdccd.cc.ca.us
Date: 1 Jul 1996 10:08:25 -0800
Subject: Hitach 450 SEM Available

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I have a working Hitachi 450 available. It was under contract until last
year. Must move it quickly to make way for another microscope. If you are
interested, please contact me at my e mail address.
I had saved it to put in one of our technical high schools which it would be
great for, however I simply don't have enough of me to go around and must
uncomplicate my life a bit.
Thanks
Judy Murphy

Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy-at-sjdccd.cc.ca.us





From: Finn-Mogens Haug :      f.m.s.haug-at-basalmed.uio.no
Date: Sat, 29 Jun 1996 15:18:09 +0200
Subject: Re: automated detection of gold particles on EM photos

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Simon,

At 14:50 30.06.96 -0400, Sanford Simon {simon-at-rockvax.rockefeller.edu} wrote:
} We are looking at large numbers of electron micrographs on which we want to
} quantitate the number of gold particles. Does anyone know of any program
} that can take an electron micrograph (or a scanned TIFF image of a
} micrograph) and count the number of gold particles?

Under DOS/Windows, "analySIS" connects to Philips, and possibly other,
TEMs and has functions for evaluating images of immunogold labelled
sections. It is sold by Soft Imaging Software, Gmbh, Germany, att.
Vasant Desai, Tel +49 251 798 000, Fax +49 251 798 00 99.
SIS just announced a website at http://www.soft-imaging-web.de

} If there is such a
} program, can it be used to count gold particles of two different sizes?
}

Yes.


Best regards, Finn-Mogens.


*****************************************************************
Finn-Mogens Haug
University of Oslo, Inst.bas.med.sci.
Department of anatomy E-mail: f.m.haug-at-basalmed.uio.no
Box 1105 Blindern Phone : +47 22 85 12 67
N-0317 Oslo, NORWAY Fax : +47 22 85 12 78







From: Garber, Charles A. :      103532.3325-at-CompuServe.COM
Date: 01 Jul 96 15:43:34 EDT
Subject: Carbon "string" vs. "rods"`

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #3.0 ] --

Randy Stoter posted the following:
=================================================
I think that you will find that a number of suppliers of carbon evaporation
} equipment now recommend carbon 'string', in preference to carbon rods. This
} 'string' is a multi filament braid and avoids precisely the type of problem
you
} mention. Additionally, I think you will find that using 'string' the whole
} evaporation process is rather more controllable - does anyone know of any
} disadvantage of carbon string (apart from requiring a different evaporator
} head)?
==================================================
There are really two "kinds" of carbon "fiber", one that is more of a "braid"
and another that is much thinner in diameter, sometimes referred to as carbon
"string" or "thead". SPI produces both products, and the "basic fiber" is the
same for both products. I will refer to both as carbon "fiber" but I am
speaking of both forms of the product. Individual carbon coaters are designed
to take either one or the other or both. The SPI Supplies carbon coaters can
use either.

It has been our own experience that under the best of circumstances, the
granularity of a carbon coating deposited with carbon rods (but in a soft and
not a diffusion pumped) vacuum is a bit smaller (e.g. slightly smaller grain
size) than what is possible using either of the two mentioned carbon fibers.
The only people who we have encountered over the years who seem to possibly find
this smaller grain size to be beneficial are those looking at submicron particle
size particles on membrane filters. There is a point where the grain size of
the evaporated carbon starts to get confused with particles of interest
collected on the filter membrane. Another advantage is that the process, at
least in the SPI carbon coaters is much faster, almost as a "flash" evaporation,
that is, it all happens within a time frame of a second (or less), and therefore
neither the sample or the "head" itself tends to have any "heating" problems.
Using the carbon rods, however, it is a slower kind of process, exposing the
sample to much more radiant heat as evidenced by the much higher temperatures
taken on by the head. And because one has to wait for the carbon rod head and
posts to "cool down" where as the carbon fiber head never really does get that
hot, the "throughput" of samples when carbon fiber coating is employed, tends to
be much faster.

The degree of control of what we call the "flash evaporation", and the ability
to reproduce coating thicknesses, sample to sample, is related to the
homogeneity along the carbon fiber, probably the most difficult parameter to
control in the manufacture of these two particular products. However, the
control is more than adequate, at least with our particular products, that
coating reproducibility does not seem to be an issue.

There is another (apparent only) disadvantage and that is the difficulty
imparting ultra high purity to the carbon fiber. With the carbon rods, the
standard purity (from SPI at least) is 5 ppm ash. However, we have never been
able to produce carbon fiber or braid down to that level and have it still
retain its desirable mechanical properties. So the purity of the fiber tends to
be on the order of 10 ppm ash, perhaps even as high as 12-13 ppm ash. However
this is still well below the level that the typical EDS user will detect any of
the impurities. So in the end, although the best purity of the carbon fiber
tends to not be quite as good as the best purity carbon rods, a "reality check"
would suggest that in the end it still would not matter to most persons. And
forgive me if this now sounds like a commerical statement, but all carbon fiber
does not come from the same place!

Further information about the two different SPI carbon fiber products can be
found in the SPI "electronic catalog" at our web site given below. Look up in
the catalog table of contents and then under "sample preparation" and then
under "evaporation supplies".

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.com

Take a look!
##########################
WWW: http://www.2spi.com
##########################
======================================================




From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 1 Jul 1996 18:39:21 -0400 (EDT)
Subject: Re: Fuji IP

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Dear Yves,

} } } Another question. What will Fuji price policy be in the future. At the
} } } moment their plates seem to be rather expensive, so that if we want to
} } } equip 5 microscopes with this system we might think twice...
} } }
} } Since the plates are reusable, it is only a one-time expense.
}
} this is not perfectly true. Fuji claims their plates are reusable 500
} times, so you may have to buy new ones every few years. It comes to be
} a bit less expensive than photo plates, but you need to add the reader's
} price. Therefore it is more expensive actually.

Can anyone with experience comfirm that the plates are reusable
500 times? Is this also true if one wishes to quantitate the response--
such as for ED intensity measurements?
}
} } } Yet another one. I would be happy to be able to get the plate out of the
} } } microscope just after taking the picture.
} } } I elaborate: with photographic plates I understand that we have to work in
} } } batches because plates are light sensitive.
} }
} } IP's are also light-sensitive--you should not expose them to fluor-
} } escent light when taking them to the reader.
}
} One of the argument of Fuji is precisely that dark room is not necessary
} any more. So here is something that I do not understand clearly. On the
} other hand I repeat that I have never seen any number printed on any IP,
} therefore it must be quite less sensitive than photo plates.
}
They are correct that a darkroom is not necessary, but it is best
to transport the IP to the reader with minimal exposure to light--espe-
cially the shorter wavelengths. I have to agree with you that the sen-
sitivity of IP's to light is less than photo's--I think I equated the
erasability of IP's by UV to sensitivity. This can be used to number the
IP's by exposing a corner, which had been previously "darkened", to a number
mask. The numbers would show up as "undark".

} } } As a consequence one has to
} } } wait till the plate is processed in order to know if it is good or not.
} } } For this reason camera makers do have a great argument telling that the
} } } image can be computer stored instantaneously.
} } }
} } } If you can get the IP out of the microscope and have it processed within
} } } minutes this would be a great improvement. It "just" takes a modification
} } } of the photo box, and I imagine that a good engineer could do that. By
} } } solving these two problems I believe that Fuji would clear any arguments
} } } against their system.
} } }
} } This is as easy as removing a single piece of film--no problem on
} } our scope. The readers I've seen take on the order of a few minutes to
} } process an IP.
}
} Right. But if you make high resolution images, you may want to know
} quickly if the photo was successful or not (focus, drift). Therefore being
} able to see the result just after taking the picture can be a real
} improvement versus normal photo plates. On a Philips microscope, removing
} one plate is the same as removing 36: you HAVE to switch off the HT. this
} means that when working in high resolution it is not so easy. The system
} I imagine would allow you to get the plate out the microscope within
} seconds, without generating any perturbation in the vacuum system.
}
I see your problem. All we have to do is air the camera. On a good
day, when the camera window is well-sealed, we could even leave the beam on
for this--except, of course, the camera door interlock won't allow it. If
we were foolish enough to defeat the interlock, we could observe the image
on our video system while the IP was removed and read. In any case, we can
turn the beam off, remove the IP, then turn the beam on again, and the image
should not have changed. If the specimen were not suseptible to drift or
radiation damage, we would be able to procede as you want to. Do you take
a through-focus series when you want hi-res?
Yours,
Bill Tivol




From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Mon, 1 Jul 1996 19:08:52 -0500
Subject: NIH Image for the PC

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For the many of you that have asked about a PC Version of NIH Image.
It has been ported to the PC by the Scion Corporation.
You may download the alpha version 5 copy from the general
NIH Image Download WWW page. The URL is:

http://rsb.info.nih.gov/nih-image/download.html

Sorry, but I've not used this version so I have no comments on
how well it works, but the people at Scion have good things in the
past to upgrade their hardware (frame grabbers for the Mac) and
modify the software to be current with NIH Image. So I would
expect this to be similiar in quality.


The NIH Image Home Page is:

http://128.231.98.16/nih-image/

Just to keep on the up and up you should all know that I have no financial
interests in Scion Corp. and just happen to be a satisifed user of their
Mac Frame Grabber Hardware.


I'll upload a copy of the PC software to the ANL Microscopy & Microanalysis
Library
in the next week or so. It will be there before the MSA/MAS/MSC-SMC meeting
in Minneapolis (August 11-15th).

Nestor
Your Friendly Neighborhood SysOp






From: Koenraad.Janssens-at-mtm.kuleuven.ac.be (Koenraad Janssens)
Date: Tue, 2 Jul 1996 07:10:50 +0200
Subject: Re: Where does the nucleus go?

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} Randy Nessler wrote:
}
} ...
} While in the tour host mode, I was
} explaining thermionic emission at the TEM. One student asked the question,
} what
} happens to the tungsten nucleus after the electrons are freed and removed?
} ...

Put in simple words, I believe the answer is like this:

The Tungsten atoms are part of a "metallic" crystal, which implies that part of
the electrons are moving freely throughout the crystal. One could envisage
this as the nuclei remaining at a fixed position while being surrounded by
a "cloud" of electrons. The electrons escaping from the tip and as such being
removed from the crystal, are simply replaced by other ones from from the power
supply. So what happens to the nuclei: nothing.

Hope this frees the mind of your students,
if so maybe you can trouble them by telling them that electrons can also
be seen as electromagnetic waves instead of particles ...

Koen Janssens

=======================================================================
| Koenraad.Janssens-at- | KULeuven MTM de Croylaan 2 |
| mtm.kuleuven.ac.be | B-3001 Leuven Belgium |
| http://www.mtm.kuleuven.ac.be/Members/Researchers/KoenraadJanssens |
=======================================================================





From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Tue, 2 Jul 1996 09:14:33 -0500
Subject: Re: Carbon "string" vs. "rods"`

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The technique of fiber evaporation as well as a comparison of granularity
of rod versus fiber depositions can be found in:

D. Vesely and S. Woodisse (1982). Carbon coating with carbon fiber
filaments, Proc. RMS 17 (3):137-139.

"Precise and reproducible deposition of thin and ultrathin carbon films by
flash evaporation of carbon yarn in high vacuum, J. Microsc., 133: 17-25
(1984),

For granularity of carbon films, please refer to H. K. Koelbel (1976).
Carbon supporting films for high resolution electron microscopy-
improvement of quality and preparation technique. Mikroskopie 31,1.

In summary: Granularity depends in high-vacuum deposition on the
predegasing conditions. If you predegase rods and yarn only shortly to dark
red (to remove organics), you do not loose internally adsorbed gas
molecules. These gas molecules are radicalized when evaporated with the
carbon and will adsorb in flight to the carbon atoms preventing their
crystallization. Therefore, yarn can produce as fine structured films as
rods that are used only once. However, if predegased carefully, yarn will
more consistently produce very fine structured films since the amount of
deposited carbon can very accurately be controlled by the lenght the amount
of yarn and the distance to the object.

Best regards Klaus

**********************************
} Randy Stoter posted the following:
} =================================================
} I think that you will find that a number of suppliers of carbon evaporation
} } equipment now recommend carbon 'string', in preference to carbon rods. This
} } 'string' is a multi filament braid and avoids precisely the type of problem
} you
} } mention. Additionally, I think you will find that using 'string' the whole
} } evaporation process is rather more controllable - does anyone know of any
} } disadvantage of carbon string (apart from requiring a different evaporator
} } head)?
} ==================================================

Charles A. Garber, Ph. D. posted

} It has been our own experience that under the best of circumstances, the
} granularity of a carbon coating deposited with carbon rods (but in a soft and
} not a diffusion pumped) vacuum is a bit smaller (e.g. slightly smaller grain
} size) than what is possible using either of the two mentioned carbon fibers.

} The degree of control of what we call the "flash evaporation", and the ability
} to reproduce coating thicknesses, ==============================



******************************************************************************
* Klaus-Ruediger Peters, Ph.D. :Molecular Imaging Laboratory *
* Director, Molecular Imaging Laboratgory : http://panda.uchc.edu/ *
* Biomolecular Structure Analysis Center : htklaus/index.html *
* University of Connecticut Health Center : *
* 263 Farmington Ave. :F r e e Access to Differential *
* Farmington, CT 06030-2017; U.S.A :Contrast Software at *
* e-Mail: Peters-at-BSAC.UCHC.EDU : http://panda.uchc.edu/ *
* Tel: (860) 679-3977; Fax: (860) 679-1989 : htklaus/DHP-Img.html#Software*
******************************************************************************







From: Milan Svoboda :      svobm-at-ipm.cz
Date: Tue, 2 Jul 1996 16:31:32 +0100
Subject: TEM foils preparation

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Has anybody experience with Model 2000 Specimen Prep System by E.A. Fischione ?

I need to prepare TEM foils from hard and brittle materials, Ti-V nitrides
and carbides at the moment. I have been looking for the best spherical grinder
for TEM discs preparation prior to ion milling and I have got offer on Model
2000 Specimen Prep System by E.A. Fischione. Description of it sounds great.
It looks
like it do the same as spherical grinder does and much more.

Thanks
Milan
-------------------------------------------
Milan Svoboda
Institute of Physics of Materials
Zizkova 22, 616 62 Brno, Czech Republic
e-mail: svobm-at-ipm.cz
-------------------------------------------





From: JOHNA-at-SCI.WFBR.EDU
Date: Tue, 02 Jul 1996 15:17:46 -0500 (EST)
Subject: Dialysis tubing & molecular sieves

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Hello folks,

Someone once wrote about putting molecular sieves into dialysis tubing to keep
the "dust" down. This sounds like a great idea so I decided to try it. My
question, however, is: how the heck do you get the tubing open in a non-aqueous
environment. I've been soaking a piece of tubing in abs. EtOH for an hour now
and it just doesn't want to open up. Any clues would be greatly appreciated.

TIA

John

___________________________________________________
| |
| John G. Aghajanian, Ph.D. |
| Worcester Foundation for Biomedical Research |
| 222 Maple Avenue |
| Shrewsbury, MA 01545 |
| |
| Tel: 508 842-8921 ext. 147, 161 |
| Fax: 598 842-9632 |
| JOHNA-at-sci.wfbr.edu |
| |
|_________________________________________________|





From: David Garrett :      DGARRETT-at-gab.unt.edu
Date: Tue, 2 Jul 1996 14:57:28 CST6CDT
Subject: LM -- Lipid stain

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Is there a method to demonstrate lipid in botanical tissue fixed in
formaldehyde, dehydrated to 70% ETOH, embedded in LR White and cold
cured.
"TIA"

***************************************
David Garrett "DGARRETT-at-GAB.UNT.EDU"
University of North Texas
Dept. Biological Sciences
(817)565-3964 Fax (817)565-4136
***************************************




From: tania-at-dynamotive.com (Tania Jones)
Date: Tue, 02 Jul 1996 13:48:37 -0700
Subject: TN5500 update

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Message-Id: {m0ubCJA-0009m8C-at-web20.mindlink.net}
X-Sender: tania-at-pop.mindlink.net
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Mime-Version: 1.0
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Hello,

Thanks to all who replied to my request for assistance for my dead TN5500.

After many phone calls from Nick Dmytryshyn from Canberra Packard, we managed
to narrow down the problem to a dead todd power supply and a loose board. The
power supply was a surprise since we had replaced the power supply a year and a
half ago. We will also be replacing the computer fans to increase the
cooling in the system, which is most likely the reason why our power supply
died so quickly.

Tania Jones
Laboratory Manager
DynaMotive Technologies





From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Tue, 2 Jul 1996 17:00:53 GMT
Subject: Re: Dialysis tubing & molecular sieves

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Hey John!

Just wet it with water and open it then dehydrate it before filling.


} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }

} Hello folks,
}
} Someone once wrote about putting molecular sieves into dialysis tubing to keep
} the "dust" down. This sounds like a great idea so I decided to try it. My
} question, however, is: how the heck do you get the tubing open in a non-aqueous
} environment. I've been soaking a piece of tubing in abs. EtOH for an hour now
} and it just doesn't want to open up. Any clues would be greatly appreciated.
}
} TIA
}
} John
}
} ___________________________________________________
} | |
} | John G. Aghajanian, Ph.D. |
} | Worcester Foundation for Biomedical Research |
} | 222 Maple Avenue |
} | Shrewsbury, MA 01545 |
} | |
} | Tel: 508 842-8921 ext. 147, 161 |
} | Fax: 598 842-9632 |
} | JOHNA-at-sci.wfbr.edu |
} | |
} |_________________________________________________|
}
}
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: Margaret Hogan :      meh-at-aretha.jax.org
Date: Tue, 02 Jul 1996 16:02:06 -0400
Subject: position available

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Message-Id: {199607022000.QAA25304-at-aretha.jax.org}
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Histology - Biomedical Technologist/ Senior Biomedical Technologist

A regular full time position is open in The Jackson Laboratory Biological
Imaging Department - Histology Laboratory. The Jackson Laboratory is a
non-profit independent laboratory founded in 1929 on the premise that the
causes of cancer and other diseases could be discovered through
Mammalian genetic research. The Laboratory specializes in mammalian
genetics using inbred laboratory mice as model systems to study health
problems such as cancer diabetes, anemia, heart disease and aging. Located
on a large island in the gulf of Maine and surrounded by Acadia
National Park, The Jackson Laboratory is currently undergoing a major
expansion of its scientific staff and its research facilities.

There is a regular, full-time position available in the Biological Imaging
Department for the Histology Laboratory. The position includes histological
techniques such as paraffin embedding, single and serial sectioning and
cryotomy in conjunction with immunohistochemistry techniques, straining
using heavy metals, as well as other special stains. Applicants must posses
a Bachelors degree or equivalent, with experience in the above techniques.
Two years related laboratory experience working with Murine specimens is
preferred. The successful candidate must be a self starter, pay attention
to detail and be able to work independently with little supervision. This
individual will be responsible for providing services in support of numerous
diverse research projects, must interact well with multiple users and work
productively in a team environment. Position will be filled at Biomedical
Technologist or Senior Biomedical Technologist level depending on background
and experience of successful applicant.

Salary range is mid to high $20,000 plus benefits and is negotiable
depending on level of experience.

Interested applicants should send CV to:

Joanne Bradt
Employment Specialist
The Jackson Laboratory
600 Main Street
Bar Harbor Maine 04609
(207) 288-3371 ext. 1281
(207) 288-3371 ext. 1082 FAX
jcb-at-aretha.jax.org





From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Tue, 2 Jul 1996 20:34:01 -0400 (EDT)
Subject: Re: LM -- Lipid stain

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On Tue, 2 Jul 1996, David Garrett wrote:

} Is there a method to demonstrate lipid in botanical tissue fixed in
} formaldehyde, dehydrated to 70% ETOH, embedded in LR White and cold
} cured.
} "TIA"
}
} ***************************************
} David Garrett "DGARRETT-at-GAB.UNT.EDU"
} University of North Texas
} Dept. Biological Sciences
} (817)565-3964 Fax (817)565-4136
} ***************************************
}
At the LM level there is as long as there is lipid remaining in the
tissue. You can demonstrate
it using several lipid dyes. Nile Red is my dye of choice at the
moment. It penetrates into all of the tissue but only flouresces in a
neutral environment (i.e. neutral lipids). Stain sections for 1-5 minutes
and then view using a flourescein filter pack on a flourescent microscope.

If the lipid has been dissolved out then of course there is no way to
detect it. One method for maintaining lipid in tissue is to complex it so
it is not removed. We have found the OTAP method particularly good for
this. Check out papers by John Guyton and Keith Klemp for details.

I hope this is useful.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************




From: fismed-at-anvax2.cineca.it (Ist. di Scienze Fisiche - Univ. Ancona - Tel.071 2204602 - Fax 071 2204605)
Date: Wed, 3 Jul 1996 12:09:52 +0200
Subject: SEM -Software analysis

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Message-Id: {199607031010.FAA05232-at-Sparc5.Microscopy.Com}

I would like to have information about software imaging analysis,
concerning asbestos applications.
I work with a Philips system.
Dr Luigi Gobbi
Istituto di Scienze Fisiche
FAX (39)-71-2204605
e-mail fismed-at-anvax2.unian.it




From: ebs-at-ebsciences.com
Date: Wed, 3 Jul 1996 06:55:37 -0500
Subject: Re: Carbon "string" vs. "rods"`

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I forwarded the inquiry regarding carbon "string" vs. "rods" to Tony King,
the Polaron product specialist at VG Microtech. Here is Tony's response:

} From: "Tony King" {tking-at-vacgen.fisons.co.uk}
} To: ebs-at-ebsciences.com
} Date: Tue, 2 Jul 1996 15:16:15 +0000
} Subject: Re: Carbon "string" vs. "rods"`

VG Microtech, as a manufacturer of carbon evaporation equipment does
"recommend carbon 'string', in preference to carbon rods," but only for
reasons of ease of use.

Actually, carbon rod evaporation should be more controllable. You try to
gauge the thickness of carbon laid down by string, but with the carbon rod
method it is possible to control the length of burn and, therefore, the
deposition.

The main reason we have come across for the rods breaking is when the rod
has been formed by sharpening, which introduces micro cracks. The carbon
rods should be ground to shape.

As Chuck Garber mentioned, "under the best of circumstances, the granularity
of a carbon coating deposited with carbon rods (but in a soft and not a
diffusion pumped) vacuum is a bit smaller (e.g. slightly smaller grain size)
than what is possible using either of the two mentioned carbon fibers." We
believe this is due to the massive deterioration in vacuum when the fibre is
flashed. The process time with rods should only be 1-2 seconds longer (at
least in the Polaron carbon coaters). If you push enough power through the
carbon rod, it will also flash instantly as the fibre appears to do.

Chuck continues, "Using the carbon rods, however, it is a slower kind of
process, exposing the sample to much more radiant heat as evidenced by the
much higher temperatures taken on by the head." This is a debatable point,
as the heating is by transmission through the metal. To evaporate, the
carbon must reach, I think, about 2700 degrees C. Mass for mass, the same
amount of energy in watts will be used. The Polaron systems have a shutter
to protect the sample during outgassing. They also have switchable power
supplies for the use of any fibre or rod.

As for the issue of relative purity, there are often higher peaks of sulpher
in the fibre (no statement toward any individual commercial product is being
made here).

Also, note that the fibre can fall as spindle-like shards onto the sample
surface if handling is rough.

} Regards,
}
} Tony King
} Product specialist
} VG Microtech/ Polaron range
}
} Tel: +44 (0)1825 746251
} Fax: +44 (0)1825 768343
}
} Disclaimer:
} The views and opinions expressed are not necessarily
} those of VG Microtech.
}

Further information about the Polaron carbon coaters can be found at the
Energy Beam Sciences web site (http://www.ebsciences.com/).
Steven Slap

********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Wed, 3 Jul 1996 09:07:24 -500
Subject: EdgeCraft Diamond Knives

Contents Retrieved from Microscopy Listserver Archives
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While in no way wishing to offend ANY of the vendors on the
listserver I have had a few inquiries from my users into EdgeCraft
Diamond knives. I have no experience with EdgeCraft, and I am
unable to provide any answer to my users (I have used many other
manufacturers knives and as with everyone else in ultramicrotomy I
have my personal favorites without any hard evidence supporting my
choice).

Does anyone out there have any experience and comments for or
against EdgeCraft knives (my interested users will doing roomtemp,
ultrathinsectioning of biological material)? Please e-mail me
directly DO NOT respond to the listserver, as I do not think it would
be fair to EdgeCraft or any other manufacturer to do so.


[ I have no personal or fianicial ties with ANY diamond knife
manufacturers or vendors]

Thank you.




Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu




From: preid-at-rsmas.miami.edu (Pamela Reid)
Date: Wed, 3 Jul 1996 11:23:57 -0500
Subject: matching funds for ESEM

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I would appreciate any ideas about foundations or other sources we might
approach to obtain matching funds for a multi-user SEM proposal
-specifically an environmental SEM- that will be submitted to NSF. The
research that will be done using this machine is wide-ranging, including
marine science, earth science and material science problems. We are aware
of Keck, and that is a possibility, but there are other proposals in line
for Keck at our university, so we can't count on that. I would be very
grateful for any suggestions!
Thanks alot
Pam Reid

__________
Dr. Pamela Reid
Research Associate Professor
University of Miami/RSMAS-MGG
4600 Rickenbacker Causeway
Miami, Fl 33149

email: preid-at-rsmas.miami.edu
phone (305) 361-4606
fax (305) 361-4632






From: melliott-at-prl.pulmonary.ubc.ca
Date: Wed, 3 Jul 1996 11:30:26 +0800PST
Subject: Krumdieck tissue slicer

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Has any one heard of a Krumdieck tissue slicer. We have found a
reference to it but can find no info on it. Any help would be
greatly appreciated.
Thanks

Mark Elliott, PhD
UBC-Pulmonary Research Lab
Vancouver BC




From: Donald Lovett :      lovett-at-trenton.edu
Date: Wed, 3 Jul 1996 16:13:36 -0400 (EDT)
Subject: Horseraddish peroxidase/DAB

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I am about to begin a study of whether certain intercellular spaces are
continuous with other spaces in crabs. All of my references suggest
using horseraddish peroxidase with DAB and H202, but they are 20+ years
old. Are there any more recent modifications of this technique? How
does this compare to Lanthanum or Ruthenium? Has anyone tried an
organism with hemocyannin? I would greatly appreciate any advice or updates.

Thank you.

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-trenton.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
Trenton State College, NJ 08650-4700 fax: (609) 771-2674






From: tara Spires :      tspires-at-uts.cc.utexas.edu
Date: Wed, 3 Jul 1996 16:37:38 -0500 (CDT)
Subject: Horseraddish peroxidase/DAB

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subscribe microscopy




From: Hewlett Bryan :      HEWLETT-at-corp.cmh.on.ca
Date: Wed, 03 Jul 1996 17:49:00 -0700 (PDT)
Subject: Horseraddish peroxidase/DAB

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Subcribe microscopy
Bryan R hewlett
e-mail hewlett-at-corp.cmh.on.ca




From: Sara Miller :      saram-at-acpub.duke.edu
Date: Wed, 3 Jul 1996 21:21:02 -0400 (EDT)
Subject: Re: EdgeCraft Diamond Knives

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Information, both positive and negative on diamond knives and other
products, is helpful to users. The most reliable information about a
product is from someone who has used it in the field. I, for one, would
welcome comments on products I may have occasion to purchase in the
future. I have no experience with EdgeCraft.

On Wed, 3 Jul 1996, Richard E. Edelmann wrote:

} Date: Wed, 3 Jul 1996 09:07:24 -500
} From: Richard E. Edelmann {edelmare-at-CASMAIL.MUOHIO.EDU}
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: EdgeCraft Diamond Knives
}
} While in no way wishing to offend ANY of the vendors on the
} listserver I have had a few inquiries from my users into EdgeCraft
} Diamond knives. I have no experience with EdgeCraft, and I am
} unable to provide any answer to my users (I have used many other
} manufacturers knives and as with everyone else in ultramicrotomy I
} have my personal favorites without any hard evidence supporting my
} choice).
}
} Does anyone out there have any experience and comments for or
} against EdgeCraft knives (my interested users will doing roomtemp,
} ultrathinsectioning of biological material)? Please e-mail me
} directly DO NOT respond to the listserver, as I do not think it would
} be fair to EdgeCraft or any other manufacturer to do so.
}
}
} [ I have no personal or fianicial ties with ANY diamond knife
} manufacturers or vendors]
}
} Thank you.
}
}
}
}
} Richard E. Edelmann
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513-529-5712 Fax: 513-529-4243
} E-mail: edelmare-at-muohio.edu
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: Probing & Structure :      pns-at-ultra.net.au
Date: Thu, 4 Jul 1996 13:47:02 +1000
Subject: Re: LM -- Lipid stain

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On Tue, 2 Jul 1996, David Garrett wrote:

} Is there a method to demonstrate lipid in botanical tissue fixed in
} formaldehyde, dehydrated to 70% ETOH, embedded in LR White and cold
} cured.
*******************
Every EM user knows that high atomic number atoms can be used to increase
contrast and therefore "stain". Lower atomic number atoms when packed into
large molecules are also denser and can be used as "stains" in EM. That is
true for quite a number of stains, including the common lipid stain Sudan
Black B.
This particular stain will in fact show as completely opaque in TEM.

Also the opposite, the loss of lipids can be demonstrated by using a
lipase to digest these. Use gold grids if incubating the sections on the grid.

Jim Darley

Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site http://www.ultra.net.au/~pns/
***********************





From: WILLI SALVENMOSER :      Willi.Salvenmoser-at-uibk.ac.at
Date: Thu, 4 Jul 1996 10:01:48 +0100
Subject: subscribe

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subscribe microscopy please

****************************************************
Willi Salvenmoser, Dept. Zoology, Univ. Innsbruck
Technikerstrasse 25, A-6020 Innsbruck, Austria
Willi.Salvenmoser-at-uibk.ac.at
tel: (43)512-507-6162 fax: (43)512-507-2930
****************************************************




From: Alan Leslie :      Alan.Leslie-at-nts.hl.siemens.de
Date: Thu, 4 Jul 1996 14:48:06 +-200
Subject: TEM Specialist Position Open

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Message-Id: {01BB69B7.D0723D60-at-NTS_PCN_0039.nts.hl.siemens.de}

The Product Engineering, Physical Failure Analysis Laboratory of Siemens =
Microelectronics Ltd, North Tyneside, UK invites applicants for a TEM =
Specialist position currently available. Ideally candidates must have a =
PhD degree, Physics or Materials Science and 5-10 years experience in =
the field of TEM. Knowledge and experience in Si semiconductors and Si =
IC Processing would be preferred but not essential. The candidate should =
be a good team worker with excellent problem solving skills and able to =
work in a high pressure manufacturing environment. The candidate should =
have the legal right to work in the UK.

The successful applicant will form part of an analytical problem solving =
team that will support the manufacturing of 200mm processed Si Wafers =
for the Microelectronics industry through materials and process =
analysis. The new wafer fab is a 1.1bn pound investment by Siemens and =
will fabricate 16MB DRAM's on 200mm Si Wafers initially, moving on to =
newer generations of DRAM and Logic Products utilising 0.25-0.35um =
technology.

Interested parties please email your CV to me at=20

alan.leslie-at-nts.hl.siemens.de

or mail or fax them to=20

Siemens Microelectronics Ltd
3 Kingfisher Way
Silverlink Business Park
Silverlink
Wallsend
Tyne and Wear NE28 9ND
UK

Tel : (44)-191-295-0300
Fax : (44)-191-295-0400

Alan Leslie
PE PFA Section Head
Siemens Microelectronics Ltd





From: Alan Leslie :      Alan.Leslie-at-nts.hl.siemens.de
Date: Thu, 4 Jul 1996 14:50:47 +-200
Subject: Auger Analyst Position Open

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Message-Id: {01BB69B8.3130C7C0-at-NTS_PCN_0039.nts.hl.siemens.de}

The Product Engineering, Physical Failure Analysis Laboratory of Siemens =
Microelectronics Ltd, North Tyneside, UK invites applicants for an Auger =
Analyst position currently available. Ideally candidates must have an =
MSc or PhD degree, Physics or Materials Science and 1-5 years experience =
in the field of Auger. Experience in other areas of Surface Science and =
Analytical Techniques is desired. Knowledge and experience in Si =
semiconductors and Si IC Processing would be preferred but not =
essential. The candidate should be a good team worker with excellent =
problem solving skills and able to work in a high pressure manufacturing =
environment. The candidate should have the legal right to work in the =
UK.

The successful applicant will form part of an analytical problem solving =
team that will support the manufacturing of 200mm processed Si Wafers =
for the Microelectronics industry through materials and process =
analysis. The new wafer fab is a 1.1bn pound investment by Siemens and =
will fabricate 16MB DRAM's on 200mm Si Wafers initially, moving on to =
newer generations of DRAM and Logic Products utilising 0.25-0.35um =
technology.

Interested parties please email your CV to me at=20

alan.leslie-at-nts.hl.siemens.de

or mail or fax them to=20

Siemens Microelectronics Ltd
3 Kingfisher Way
Silverlink Business Park
Silverlink
Wallsend
Tyne and Wear NE28 9ND
UK

Tel : (44)-191-295-0300
Fax : (44)-191-295-0400

Alan Leslie
PE PFA Section Head
Siemens Microelectronics Ltd






From: Kerry Gascoigne :      Kerry.Gascoigne-at-flinders.edu.au
Date: Fri, 5 Jul 1996 13:32:55 +0930
Subject: Subscription

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*****************************************************
Kerry Gascoigne
Flinders Microscope and Image Analysis Facility.
Ph (08)204-4858 Fax (08)277-0085
***************************************************




From: Ian Harrowfield :      Ian.Harrowfield-at-minerals.csiro.au
Date: Fri, 5 Jul 1996 16:52:45 +1000
Subject: Unsubcribe

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Ian Harrowfield - CSIRO _--_|\ Ian.Harrowfield-at-minerals.csiro.au
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3207 AUSTRALIA

WWW site http://www.minerals.csiro.au/em-unit/





From: Ian Harrowfield :      Ian.Harrowfield-at-minerals.csiro.au
Date: Fri, 5 Jul 1996 16:52:45 +1000
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3207 AUSTRALIA

WWW site http://www.minerals.csiro.au/em-unit/





From: Dane Gerneke :      dane-at-uctvms.uct.ac.za
Date: Fri, 05 Jul 1996 14:50:59 -0500
Subject: Carbon debate

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-- [ From: Dane Gerneke * EMC.Ver #2.5.02 ] --

Greetings all

An additional spoonful of carbon

It seem the debate centers around the assumptions of resolution and coating
flat polished samples.

We still have a need to carbon coat topographic samples for EDS. In which
case resolution does not enter the image when one is using barge pole probe
diameters to get enough signal. It is difficult enough having to view
samples with the relatively poor carbon coating and this is made worse if
there is high topography. Directional coating results in a preferential
coating of the surfaces facing the carbon source. The solution is to have a
rotating sample holder that effectively presents all aspects of the sample
to the source at some point in time. This thus requires the carbon source to
evaporate over a reasonably long period of time and can not be achieved by
"flash" evaporation. So rods remain the best.

I agree with Tony King that rods are more controllable, and, can suit more
applications - from making very thin carbon films to topographic coating. If
one is only coating flat polished samples for microanalysis and wish to
reduce operator and other variables then carbon string may the better method
for standardization.

Regards

Dane Gerneke
E M Unit (Southern most EMU in Africa)
University of Cape Town
Tel + 27 21 650 2819
Fax + 27 21 6891528
e-mail IN%"dane-at-uctvms.uct.ac.za"




From: Bart Cannon :      cannonmp-at-accessone.com (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Fri, 5 Jul 1996 08:12:35 -0500
Subject: SEM Digital Imaging

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Message-Id: {199607051308.IAA01125-at-Sparc5.Microscopy.Com}
X-Sender: zaluzec-at-microscopy.com
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I am seeking assistance from those who have interfaced analog SEMs to
IBM PCs for the purpose of digital image acquisition for under $5,000.

My instrument is an ARL SEMQ electron microprobe. It has easily
accessed blanking signals as well as external X and Y scan drive inputs.

Numerous manufacturers offer suitable hardware solutions, but the major
obstacle to my implementation is development of a Windows 95 software
interface. I need 3 drop down menus: resolution, scan speed and file
save.

I am not a programmer. Has anyone developed code for the interface to a
particular board? Would someone with prior expertise be willing to
consult for a fee for me?

Thank you very much.

Bart Cannon
Cannon Microprobe
1041 NE 100th Street
Seattle, WA 98125

206 522-9233 or 3947 fax

cannonmp-at-accessone.com







From: Elaine M. Mohrbach :      emohrbac-at-MOOSE.UVM.EDU
Date: Fri, 5 Jul 1996 09:26:54 -0400 (EDT)
Subject: Rock polishing for SEM

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I have a rough rock (Silicate) which I wish to polish and put in the SEM.
Is there a method for polishing, either mechanical or chemical?
Is there a lab which does this type of work routinely to which I might
send my sample for preparation?
I would appreciate hearing any of your ideas.

Elaine Mohrbach
Cell Imaging
Univ. of VT





From: Raymond F Egerton :      egerton-at-phys.ualberta.ca
Date: Fri, 5 Jul 1996 09:56:44 -0600 (MDT)
Subject: Microsc. Soc. of Canada

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Papers from the 22nd Annual Meeting of the Microscopical Society of Canada
(Ottawa, 1995) are published in Volume 26, Issue #6 of MICRON. This includes
review papers on structure determination of proteins (D. L. Dorset) and on
low-energy point-source microscopy (H.J. Kreuzer) + 10 research papers.

The 24th Annual Meeting of MSC will be held in Edmonton, 4 - 7 June 1997,
and will include symposia on developments in SEM, SPM, confocal
microscopy, energy-filtered TEM and microscopy of dynamic processes. For
further information, see web page http://www.ualberta.ca/~mmid.mschome.html
or contact me at the address below.

Ray Egerton, Physics Department, University of Alberta, Canada T6G 2J1
Phone: 403-492-5095, FAX: 403-492-0714, e-mail: egerton-at-phys.ualberta.ca
------------------------------------------------------------------------






From: smithg-at-gar.union.edu (George Smith)
Date: Fri, 5 Jul 1996 09:49:54 -0500
Subject: Oil Diffusion Pump

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Message-Id: {v01510101ae02d3296153-at-[149.106.38.10]}
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Hello to Everyone:
I am in need of an oil difusion pump. The "tree" in the older one was
destroyed, but the "housing" remains in good condition. It is highly
unlikely that only a "tree" will be found, even though that is all I need.
Therefore I am looking for the complete unit.
It goes on a Hitachi HUS-4 vacuum evaporator. The pump is model #DPF
25- 7-37 Chome Minami Synamachi - Koto-ku Tokoyo, Japan.

Some specs on the machine include:
(1) from bottom of unit to top of flange 12-1/4" high
(2) flange is 1-1/4" thick (probably not very important)
(3) inside diameter is 2-1/2"

Writings on side of the pump:
(1) Final vol. = 3 x 10-7 mm hg
(2) Speed, pumping = 15 l/sec
(3) Power = 350 watt
(4) Oil change = 60 cc

If anyone out there knows where I might be able to find one of these,
your response will be greatly appreciated. It is also possible that I may
be able to substitute another pump of similar size and capacity.
*******************
George Smith, Ph.D.
Union College
Schenectady, NY 12302
smithg-at-gar.union.edu
(518)374-4907
******************

George W. Smith, Ph.D.
Dept. of Biology
Union College
Schenectady, NY 12308






From: slakmon-at-scottscientific.com (P. Slakmon)
Date: Fri, 5 Jul 1996 13:59:27 -0400 (EDT)
Subject: Time lapse video system

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I would like to know if anyone out there have experience from video time
lapse tape recorders, especially in conjunction w video microscopy.
I4m interested in buying such a system but don't know all alternatives and
their pros and cons. All information of interest.

Thanks in advance



=============================================
Mikael Gustafsson MD, PhD
Dept Med. Microbiology and
Dept Internal Medicine, Cardiology section
University Hospital of Linkoping
S 581 85 LINKOPING
SWEDEN

E-Mail: MikGu-at-mme.liu.se
FAX: 046/13/224789
Phone: 046/13/224783
=============================================


_______________________________________________
SCOTT SCIENTIFIC
P.O. Box 66552 Station Cavendish
Montreal, Quebec, H4W 3J6, Canada

Telephone: 514-485-2309 Fax: 514-485-9931
Voice Mail: 514-888-6509

WWW Site: http://www.scottscientific.com

E-Mail: info-at-scottscientific.com
sales-at-scottscientific.com
admin-at-scottscientific.com
links-at-scottscientific.com
_______________________________________________





From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Fri, 5 Jul 1996 09:15:46 -0500
Subject: Re: Horseraddish peroxidase/DAB

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Message-Id: {199607051411.JAA15018-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
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} I am about to begin a study of whether certain intercellular spaces are
} continuous with other spaces in crabs. All of my references suggest
} using horseraddish peroxidase with DAB and H202, but they are 20+ years
} old. Are there any more recent modifications of this technique? How
} does this compare to Lanthanum or Ruthenium? Has anyone tried an
} organism with hemocyannin? I would greatly appreciate any advice or updates.
}
} Thank you.
}
} ______________________________________________________________________
} Donald L. Lovett e-mail: lovett-at-trenton.edu

LaNO3 worked in tracing intercellular spaces in amphipods, but also
appeared to penetrate into the (sensory receptor) cell, near the ciliary
roots. The reference is buried in boxes, but it was VJ Steele, Journal of
Morphology, on the anatomy of the Organ of Bellonci in _Gammarus_ (I forget
the exact title), year was around 1985, definitely between '84-'87. Sorry I
can't do better on the reference.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********






From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Fri, 5 Jul 1996 09:45:43 -0500
Subject: Re: Carbon debate

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Message-Id: {199607051441.JAA21155-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Directional coating results in a preferential
} coating of the surfaces facing the carbon source. The solution is to have a
} rotating sample holder that effectively presents all aspects of the sample
} to the source at some point in time. This thus requires the carbon source to
} evaporate over a reasonably long period of time and can not be achieved by
} "flash" evaporation. So rods remain the best.
}
} Dane Gerneke

I disagree that carbon string can only be flash-evaporated. We use
carbon cord (braided string, fairly thick), and this glows for long enough
to evenly cover a rotating sample of complex topography. The only trick is
to have to sample rotating before you start applying current to the cord,
and to apply the current moderately slowly ("slow, but a little faster than
not-too-slow", the usual sort of quantitative method). This lets the cord
glow white-hot for some seconds before burning through, long enough that
the current can be turned down before burn-throug. The coating thickness
can be controlled this way.
We haven't used carbon rods for some while now, and our users seem
pleased with their results
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********






From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Fri, 5 Jul 1996 13:10:26 -0500
Subject: Re: Rock polishing for SEM

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Message-Id: {199607051805.NAA02581-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} I have a rough rock (Silicate) which I wish to polish and put in the SEM.
} Is there a method for polishing, either mechanical or chemical?
} Is there a lab which does this type of work routinely to which I might
} send my sample for preparation?
} I would appreciate hearing any of your ideas.
}
} Elaine Mohrbach
} Cell Imaging
} Univ. of VT
}

There are two approaches, depending on who you know:
1) Go over to the geology department, they ought to have rock saws, flat
laps, and everything else you need.
2) Try an amateur rockhound or lapidary--they will also have the
equipment needed. There is probably a lapidary club in town.
Either way, whoever does the sawing and grinding will need to end
up with an extra-fine (or finer) grit diamond lap, *probably*. With some
minerals, other saws/laps are better. The soft minerals give the most
trouble, but in Vermont (if I read my screen font right), you should be
dealing with hard minerals.
Geologists also usually embed rock samples in epoxy for the final
polish, and to hold the specimen in the scope, but if you don't mind
smearing the sides with silver or carbon paint/paste, an vise-type holder
for irregular specimens works fine.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********






From: zaluzec-at-sparc5.microscopy.com (Nestor J. Zaluzec)
Date: Sat, 6 Jul 1996 16:13:05 -0500
Subject: Update : Microscopy & Microanalysis Program On-Line

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To: Microscopy ListServer Subscribers and
All Society Members of MSA, MAS, MSC/SMC

The Microscopy & Microanalysis 96 Program Booklet will
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about July 15th, 1996. This booklet will contain the
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and times of all sessions and other related information.

For those of you who wish on-line assess the entire
program database (Authors, Paper Titles, Session Dates,
Times and Rooms) is now also a searchable index on the MSA
WWW site at the URL:

http://WWW.MSA.Microscopy.Com

Look for the Hot Link called "M&M96 Program Search Engine"

You may search the M&M 96 Program by various keywords, Day of the
Week or Authors name and the relevant portion of the database
will be delivered to your screen in a concise tabular format
(requires NetScape V 2.0 or Tables compatible browser).

As this mailing reaches more than just the Microscopy
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From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Sun, 7 Jul 1996 19:51:38 -0300 (EST)
Subject: LM: antibodies against bovine lymphocytes

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I need to buy some mouse or rabbit antibodies against bovine lymphocytes
(CD3, CD4, etc.)for immunohistochemistry purposes. We have just received
the money to buy it by direct importation.
We will be very grateful for any information.
=============================================================================
Francisco Javier Hernandez Blazquez |
Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-spider.usp.br
Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265
Departamento de Ciencias Basicas/Histologia| r. 278
Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 618606
CEP 13630-000 Pirassununga (Sao Paulo) |
BRAZIL |
==============================================================================






From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Sun, 7 Jul 1996 19:36:14 -0300 (EST)
Subject: Re: Horseraddish peroxidase/DAB

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You may use horse spleen ferritin. It may be observer with LM (revealed
by Perls reaction) and with EM.

On Wed, 3 Jul 1996, Donald Lovett wrote:

}
} I am about to begin a study of whether certain intercellular spaces are
} continuous with other spaces in crabs. All of my references suggest
} using horseraddish peroxidase with DAB and H202, but they are 20+ years
} old. Are there any more recent modifications of this technique? How
} does this compare to Lanthanum or Ruthenium? Has anyone tried an
} organism with hemocyannin? I would greatly appreciate any advice or updates.
}
} Thank you.
}
} ______________________________________________________________________
} Donald L. Lovett e-mail: lovett-at-trenton.edu
} Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} Trenton State College, NJ 08650-4700 fax: (609) 771-2674
}
}
}





From: Corvos-at-aol.com
Date: Sun, 7 Jul 1996 20:42:28 -0400
Subject: Radon Detectors

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All,

Does anyone have an address or phone number for Pylon. They manufacture
radon detectors...

Thank you,

Walter Protheroe
E-MAC




From: Michael Bosma :      Michael.Bosma-at-vf.slu.se
Date: Mon, 8 Jul 1996 11:51:12 +0200
Subject: Radon Detectors

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unsubscribe
******************************************************************************
* *
* Michael Bosma Telephone: +46 418 670 62 *
* The Swedish University of Fax: + 46 418 670 81 *
* Agricultural Sciences E-mail: Michael Bosma-at-vf.slu.se *
* Dept of Plant Breeding Research *
* S-26831 Svaloev, Sweden *
* *
******************************************************************************





From: Shane Roberts :      spatrob-at-earthlink.net
Date: Mon, 8 Jul 1996 14:10:13 -0400 (EDT)
Subject: LiNbO3 crystals

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Does anyone have any information regarding the cutting and polishing of
LiNbO3 crystals?

Thanks in advance.

Shane Roberts
Shane Roberts





From: Shane Roberts :      spatrob-at-earthlink.net (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Mon, 8 Jul 1996 16:55:14 -0500
Subject: Cutting and polishing LiNbO3

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Does anyone have any information / experience in cutting and
polishing LiNbO3 crystals? Any help would be greatly appreciated.
Thanks in advance.

Shane Roberts
Shane Roberts








From: FGask99200-at-aol.com
Date: Mon, 8 Jul 1996 18:55:30 -0400
Subject: Address Changed

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Sorry for the bother but can you changed my E-Mail address imediately to :
fgask-at-juno.com

Thanks,
fgask-at-juno.com




From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Mon, 8 Jul 1996 18:02:07 -0300 (EST)
Subject: Re: ANTIBODIES AGAINST BOV. LYMPHOCYTES (fwd)

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Thank your for your kindness in reply my mail.
I have already hunt the Serotec address and phone but I
failed all times I've tried.

On Mon, 8 Jul 1996 bruyntjes-at-hvvc03.voeding.tno.nl wrote:

} Hi
}
} There is a comany called Serotec in Europe, Canada and/or USA who
} deliveres antibodies against bovine lymphocytes (CD1, CD2, CD4, CD6, CD8,
} CD14, CD45.
} I hope this will help you,
}
} Joost Bruijntjes
} TNO Zeist
} Holland
} E mail: bruyntjes-at-voeding.tno.nl
}
}






From: andrew :      andrew-at-gaia.coppe.ufrj.br
Date: Mon, 08 Jul 1996 22:00:44 -0700
Subject: Micromat 96 Brazil

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Dear Colleagues,

For more details on the:

5th. Brazilian Conference on
Microscopy of Materials
Rio de Janeiro, Brazil
13-16 October 1996

please consult the home page of the event on:

http:/www.rdc.puc-rio.br/eventos/Micromat96/mmatmain.htm

Best regards from the Organizing Committe




From: Zenon Rajfur :      sfrajfur-at-rci.rutgers.edu
Date: Tue, 09 Jul 1996 10:18:11 -0700
Subject: (no subject)

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Subscribe Microscopy
My e-mail address is : chj-at-rci.rutgers.edu




From: Kim Rensing :      krensing.uvic.ca-at-cynic.ns.uvic.ca
Date: Tue, 09 Jul 1996 08:27:57 -0700
Subject: spamming

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Message-Id: {199607091530.IAA14196-at-cynic.ns.uvic.ca}
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I find the multiple messages sent by calarco-at-bu.edu very close to spamming.
I am not a U.S. citizen and subscribe to this listserver to keep abreast on
microscopy. Please refrain from this in the future. Thanks.

Kim Rensing





From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Tue, 9 Jul 1996 13:45:44 -0400
Subject: Re: for calarco@bu.edu RE: Attention 50States

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Bill ,
I fully agree with you, let's try and keep it to micrscopy!

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: tania-at-dynamotive.com (Tania Jones)
Date: Tue, 09 Jul 1996 10:43:25 -0700
Subject: EDX: TN5500 problem revisited

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Message-Id: {m0udgjv-000CwVC-at-web20.mindlink.net}
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hello,

as i mentioned before, we had trouble booting up our TN5500 system. we replaced
the power supply and reseated one of the boards and our problems looked like
they were solved....
however, it looks like i'm back at it, trying to get the system to boot again.
the power supply is working fine and i have cleaned the contacts and reseated
several boards with no luck. keyboard lights come on and the drives are being
accessed, so i figure the problem probably lies in the floppy disks. i've tried
using several of the bootable disks i have, but no luck.

if you have any suggestions (and if it is a problem with the disks, do you know
where i can get 8 inch floppy disk versions of "new" disks) i would be very
grateful.

thank you in advance,

tania jones
laboratory manager
dynamotive technologies corp.





From: GARONEL-at-cliffy.polaroid.com
Date: Tue, 09 Jul 1996 16:25 -0400 (EDT)
Subject: EDX: TN5500 problem revisited

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For Sale

Buehler Polishing Equipt. (3 platter table) in fair condition
Dark Room Equipt. (plate and print processor) print processor in good
condition, plate processor in fair condition
Buehler cut-off saw and grinder belt - old but functioning
LKB Ultramicrotome - in great condition
Philips Diffractometer (eucentric goniometer, warhaus camera, laue
camera and track, debye-scherrer camera (may need new generator and
x-ray tube)

Pls. Contact Lynne Garone (GARONEL-at-Polaroid.com)
or 617 386-1446.




From: Vladimir Dusevich :      dusevich-at-astro.ocis.temple.edu
Date: Tue, 9 Jul 1996 09:39:31 -0400 (EDT)
Subject: Tungsten wire

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Where can we buy tungsten wire of 0.012 inch diameter?


dusevich-at-astro.ocis.temple.edu




From: Zenon Rajfur :      sfrajfur-at-rci.rutgers.edu
Date: Tue, 09 Jul 1996 10:21:23 -0700
Subject: (no subject)

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Subscribe Microscopy
e-mail)chj-at-rci.rutgers.edu




From: waheeschen-at-dow.com (Bill Heeschen 517-636-4005 Materials/ASL)
Date: Tue, 9 Jul 1996 09:21:32 -0400
Subject: for calarco@bu.edu RE: Attention 50States

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I am sure that you are well-meaning in your efforts to move the affected
people to action regarding the NSF Academic Research Infrastructure
Program budget, but I, for one, do not like having my electronic mailbox
filled with redundant and/or irrelevant messages. One message with a list
of all affected states and contacts would have been plenty. They consume
bandwidth and disk space, irrespective of the ease of deletion. I cannot
speak for the rest of the Microscopy list, but please do not send any more
of these state-by-state messages to me, either personally or through the
Microscopy list. Let's stick to microscopy and related technology.

Bill
==========================
Bill Heeschen/Analyt. Sci.- Mat. Char. -----
1897-F Building / The Dow Chemical Co. --- ---
Midland, MI 48667 U.S.A. --- D O W ---
phone: (517)636-4005 fax: (517)636-5453 --- ---
Email: waheeschen-at-dow.com -----
========================== R




From: Ciara Mullan :      mullanc-at-mcmail.cis.mcmaster.ca
Date: Tue, 9 Jul 1996 17:43:01 -0400 (EDT)
Subject: NSF & Instrumentation ALERT

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Unsubscribe
Ciara Mullan




From: Murphy, Judy :      murphy-at-sjdccd.cc.ca.us
Date: 9 Jul 1996 15:38:40 -0800
Subject: Hitachi 450 gone

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Message-ID: {n1375195730.85342-at-sjdccd.cc.ca.us}

Thank you all for your inquiries. I had so many, I decided to write a general
note. Also so everyone knows that it is no longer available. It is packed up
and going out the door Friday, which met my time schedule to move another
instrument in. It is going to be used for an outreach program, which I am
happy about as that was my original intention for the instrument.
Thank you again for all those that responded.
Have a happy summer. Perhaps I will see you at MSA if you will be there.

Judy Murphy

Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy-at-ms.sjdccd.cc.ca.us





From: GARONEL-at-cliffy.polaroid.com
Date: Tue, 09 Jul 1996 14:45 -0400 (EDT)
Subject: Hitachi 450 gone

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Job Description : Polymer Microscopist

Scientist / Senior Scientist - Analytical and Materials
Characterization Laboratory - Polaroid Corp.


The Analytical and Materials Characterization Laboratory at Polaroid
Corp. has a need for a microscopist with a strong background in
polymeric and crystalline materials to join our Microscopy and Surface
Analysis Group. Experience in transmission electron microscope
operation and sample preparation is essential. Experience in scanning
electron microscopy, light microscopy, x-ray photoelectron
spectroscopy (XPS) and x-ray diffraction is desirable.
Strong interpersonal, documentation and communication skills are
necessary. Strong computer skills with application to digital imaging
and image analysis are desirable.

This position requires a Ph.D. in chemistry, materials science,
physics or engineering with training in polymer science or a M.S. with
equivalent training.

Supervisor : Lynne Garone, e. mail: GaroneL-at-Polaroid.com

Location: W4-1D
1265 Main St.
Waltham, Ma. 02254
(617) 386-1446






From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Tue, 9 Jul 1996 11:09:32 -0500
Subject: NSF alerts

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Message-Id: {v01540b00ae0833869e02-at-[128.206.15.189]}
Mime-Version: 1.0
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I am all for lobbying congress for increased funds but perhaps you could
limit your postings to "Attention United States" rather than sending 50
individual copies of the same message. It is a little annoying especially
for those of us getting legislative alerts via other sources.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: geoffa-at-amsg.austmus.gov.au (GeoffA)
Date: Wed, 10 Jul 1996 09:39:39 +1100
Subject: calarco and the 50 states

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Being over the other side of the world, this message will probably hit you a
little out of phase and after much has been said and the matter probably put
to rest (Nestor is on the ball and pretty quick at sorting this sort of stuff
- Hi Nestor!).

It is very tempting to suggest that all on the Microscopy Listserver simply
hit the Reply icon on all these messages to demonstrate how effectively email
to a listserver can amplify a single message. But this would be irresponsible
too, and I could not endorse such actions :-).

Geoff Avern




From: Smith, Peter :      SMithP-at-agresearch.cri.nz (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Tue, 9 Jul 1996 21:08:24 -0500
Subject: Fluorescence-counter stain

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Message-Id: {199607100204.VAA02861-at-Sparc5.Microscopy.Com}
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We have been using a Brdu cell proliferation kit which utilises an FITC
labelled antibody on paraformaldehyde fixed parrafin sections.We are looking
for a counterstain so that we can view the same sections under brightfield
conditions, particularly the nucleii. Haematoxylins seem to strip the FITC
from the antiobody, any suggestions would be most welcome.

Peter Smith
AgResearch Wallaceville
Upper Hutt
New Zealand







From: Simon.Dumbill-at-aeat.co.uk (Simon Dumbill)
Date: 09/07/96 11:09
Subject: NSF alerts

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Here, here..

A little annoying, too, for non-US microscopists.


______________________________ Forward Header __________________________________


I am all for lobbying congress for increased funds but perhaps you could
limit your postings to "Attention United States" rather than sending 50
individual copies of the same message. It is a little annoying especially
for those of us getting legislative alerts via other sources.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Hazel Richardson :      hazel-at-cursci.co.uk
Date: Wed, 10 Jul 1996 13:10:00 +0100
Subject: subscription

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Message-Id: {199607101115.MAA07054-at-styx.cursci.co.uk}


Please unsubscribe Hazel Richardson




From: Campbell36-at-aol.com
Date: Wed, 10 Jul 1996 10:19:08 -0400
Subject: Re: Chromium foil

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In a message dated 96-07-08 17:46:57 EDT, chuang-at-CCS.CARLETON.CA (Cheng
Huang) writes:

{ { Subj: Chromium foil
Date: 96-07-08 17:46:57 EDT
From: chuang-at-CCS.CARLETON.CA (Cheng Huang)
To: microscopy-at-Sparc5.Microscopy.Com

We are looking for chromium foil or thin wire for evaporative coating
on biological specimens with our cryo-SEM. However, companies we
talked to only sell chromium chips. If anyone has any information
regarding this, please let us know. Thank you very much in advance.
} }
There are a couple of sources for the types of evaporation materials you are
looking to acquire. R.D. Mathis Co. offers chrome plated tungsten rods (Part#
CRW-1). These rods are widely used in the optics and electronics industry.
This would be the economical solution if your evaporation power supply has
the correct voltage current relationship for the evaporation of this form of
the material. The telephone number for RD Mathis is 310-426-7049. Your
second source of chromium foil is Goodfellow. The foils are very expensive;
however, if they work they may be what you need. Goodfellow's telephone
number is 1-800-821-2870. One other option you may want to look into is
adapting you cryo-SEM stage for the sputter deposition of these materials.
You did not indicate the stage you are using, however, I know Oxford is
achieving some excellent results with this approach.

best regards,
Jim Campbell
Denton Vacuum, Inc
609-439-9100




From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Wed, 10 Jul 1996 09:10:24 -0500
Subject: lobbying

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Message-Id: {199607101405.JAA20176-at-ux1.cso.uiuc.edu}
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It's a quandry. I agree with the people who think that the list should
remain for microscopy & microscopy-related items. But then funding cuts
that eliminate money for microscopy (equipment or otherwise) *are*
microscopy related. But then, this is an international list, so why should
people in other countries have to be bothered by our government's actions?
But then, collaboration in science is international, and if a scientist in
the US can't get $$ to buy/replace equipment, a scientist in Germany/Japan,
etc. may not get the research done. Etc.
There must be a means to get scientists to act as a *community* on
issues such as funding, support of education by governments, and all the
rest of the messy stuff we don't like to get involved with. And therefore
suffer from *because* of that lack of involvement. This must be done both
on a national and international basis.
Mail servers such as the microscopy list must be kept free of
politics, or they will get buried in the swill of politics.
So maybe there should be two microscopy servers, "A" & "B", one
strictly for microscopy as now, the other for other issues, such as
funding, legislation, education, and the other things that go into making a
community.
I'm } sure { Nestor's looking for something else to do...
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********






From: Mark Wall :      Mark.Wall-at-quickmail.llnl.gov
Date: 10 Jul 1996 09:19:57 -0700
Subject: Software help

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Message-ID: {n1375131967.45195-at-quickmail.llnl.gov}

Can any one tell me if my MacII versions of Diffract 1.3 and Crystal 2.29
(stereographic projection stuff) will work on a PowerMac when we get one?
Regardless, who currently distributes these?? Thanks in advance for your help.


Mark A. Wall
LLNL
510-423-7162
or just "reply"





From: bergrh-at-msuvx2.memphis.edu (R. Howard Berg)
Date: Wed, 10 Jul 1996 12:47:59 -0600 (CST)
Subject: Hardcopy from time lapse VCR tape

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The following is posted for a colleague:

I have recorded the motion of motile hormogonia of the thermophilic
cyanobacterium Mastidocladis laminosus with a GYYR Time Lapse VCR Model
#TLC 2051-232. According to the machine's manual, the signal recorded on
the T-120 cassette is NTSC.

I request advice about how I can take individual frames from the VCR tape
and transform them into publishable quality glossy or half-tone prints.


R. Howard Berg, Ph.D.
Biology Department
University of Memphis
Memphis, TN, 38152
E mail: bergrh-at-cc.memphis.edu
phone: 901-678-4449 fax: 901-678-4457







From: ramd-at-beta.lanl.gov (Ram Devanathan)
Date: Wed, 10 Jul 1996 12:52:02 -0600 (MDT)
Subject: Lobbying

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Simple solution:
Set up a web home page for lobbying and post the URL here,
instead of sending 50 messages to the list--one for each state.
Regards,
Ram Devanathan ramd-at-lanl.gov




From: H. ADAMS :      hadams-at-nmsu.edu
Date: Wed, 10 Jul 1996 13:45:22 -0600 (MDT)
Subject: Lobbying

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Microscopists, a microbiologist came by today and asked if I knew
anything about "goniometric eyepieces". He wants to determine the
hydrophobicity of bacterial colonies by measuring the contact angle of
water droplets ricocheting off the surface of the colonies using this
eyepiece on a monocular microscope. Is anyone familar with this technic
and/or knows where one can get such an eyeiece? Also, are there other
methods to determine hydrophobicity? Any help or leads would be appreciated.

Thanks, Hank Adams
EML,
NMSU
las Cruces NM THE DROUGHT IS OVER IN NEW MEXICO!! YEH




From: andrew :      andrew-at-gaia.coppe.ufrj.br
Date: Wed, 10 Jul 1996 18:02:20 -0700
Subject: Micromat 96 - Brazil

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Message-ID: {31E4529C.7807-at-gaia.coppe.ufrj.br}

Dear Colleagues,

For more details on the:

5th. Brazilian Conference on
Microscopy of Materials
Rio de Janeiro, Brazil
13-16 October 1996

please consult the home page of the event on:

http:/www.rdc.puc-rio.br/eventos/Micromat96/mmatmain.htm

Best regards, Prof. Ivani de S. Bott (Organizing Committe Member)

PS please note the capital letter "M" in the address (.../Micromat96/..)
some people have had problems getting through because the address IS
case-sensitive and they hadn't noticed this detail.




From: akracher-at-iastate.edu (Alfred Kracher)
Date: Wed, 10 Jul 1996 13:23:59 -0600
Subject: Politics & appropriate topics

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My 2 cents on "keeping to microscopy": It *is* appropriate (in my opinion)
to alert list members if some really monumental crisis endangers funding
for most facilities. The key is (1) a brief alert, with reference to a web
site or another server to get details, (2) only if the circumstances are
really unusual. What is *not* appropriate is to clog the pipeline with a
multiplicity of nearly identical messages, regardless of whether they are
political or histological. Perhaps it should be a rule of thumb that the
more remote a topic is from miscroscopy, the shorter the message.

-----------------------------------------
Alfred Kracher
Geological Sciences
Iowa State University
Ames, IA 50011-3212
akracher-at-iastate.edu
http://www.public.iastate.edu/~akracher
vox:515 294 5439 fax:515 294 6049
-----------------------------------------






From: kszaruba-at-MMM.COM (Karen S. Zaruba)
Date: Wed, 10 Jul 1996 14:26:00 -0500
Subject: Re: lobbying

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Message-Id: {199607101921.AA207486462-at-pigseye.mmm.com}
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I agree with Philip Oshel that perhaps there should be one forum for
scientific/technical microscopy issues, and a second for political/social
microscopy issues:

} . . . So maybe there should be two microscopy servers, "A" & "B", one
} strictly for microscopy as now, the other for other issues, such as
} funding, legislation, education, and the other things that go into making a
} community. . . .

Also my 2 cents worth on lobbying: I would like to think that as scientists
we would not let ourselves become so self-interested that we would just jump
on the funding bandwagon whenever the issue came up. After all there is
only so much money to go around (less and less all the time!!) and people
need to work together to figure out where it is MOST needed. [Pause for
group hug] Ok, so I don't have a clue where that is (neither do I want to
get too preachy) but it bothers me to be TOLD to complain about funding cuts
without giving any thought to the issues.

Karen


Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
3M Company
3M Center 270-1S-01 Phone: 612-737-2971
St. Paul, MN 55144-1000

These opinions are my own and may not represent those of 3M.







From: kszaruba-at-MMM.COM (Karen S. Zaruba)
Date: Wed, 10 Jul 1996 14:26:00 -0500
Subject: Re: lobbying

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Message-Id: {199607101921.AA207486462-at-pigseye.mmm.com}
X-Mailer: Windows Eudora Version 1.4.3
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I agree with Philip Oshel that perhaps there should be one forum for
scientific/technical microscopy issues, and a second for political/social
microscopy issues:

} . . . So maybe there should be two microscopy servers, "A" & "B", one
} strictly for microscopy as now, the other for other issues, such as
} funding, legislation, education, and the other things that go into making a
} community. . . .

Also my 2 cents worth on lobbying: I would like to think that as scientists
we would not let ourselves become so self-interested that we would just jump
on the funding bandwagon whenever the issue came up. After all there is
only so much money to go around (less and less all the time!!) and people
need to work together to figure out where it is MOST needed. [Pause for
group hug] Ok, so I don't have a clue where that is (neither do I want to
get too preachy) but it bothers me to be TOLD to complain about funding cuts
without giving any thought to the issues.

Karen


Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
3M Company
3M Center 270-1S-01 Phone: 612-737-2971
St. Paul, MN 55144-1000

These opinions are my own and may not represent those of 3M.







From: Zenon Rajfur :      sfrajfur-at-rci.rutgers.edu
Date: Wed, 10 Jul 1996 20:08:52 -0400
Subject: Lobbying

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Unsubscribe microscopy sfrajfur-at-rci.rutgers.edu




From: melliott-at-prl.pulmonary.ubc.ca
Date: Wed, 10 Jul 1996 13:53:33 +0800PST
Subject: staining

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I was asked by someone in our lab to post the following.

Mark Elliott


Recently, I have experienced two kinds of staining problems:

1. background staining &
2. peppery lead precipiate staining on cytoplasm.

The routine post-stains that we use are uranyl acetate and Sato's
lead. This method never used to give any background staining.
Occasionally we get peppery lead precipitate on cytoplasm of epon
sections, as described in textbooks.

Meanwhile, this kind of background staining is most prominent with
epon sections and not as bad with Spurr's sections. So far, the LR
White sections from human biopsies have not shown much of this
problem.

I have checked for all possible causes including staining duration,
cleanliness of boat, of washing distilled water, and of tools. If I
were to suspect some of the Epon ingredients that might have gone
out-dated, same might have happened to the Spurr's.

I have tried some standard methods described in textbooks for removing
stain contamination but they did not seem to work that well.

Can anyone offer any advice on how I can go about solving this problem
or removing background stains?

Thank you!


Fanny Chu
Pulmonary Research Lab
St. Paul's Hospital
Vancouver, BC
Canada




From: kszaruba-at-MMM.COM (Karen S. Zaruba)
Date: Wed, 10 Jul 1996 13:45:22 -0500
Subject: Re: Fluorescence-counter stain

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Message-Id: {199607101840.AA151664024-at-pigseye.mmm.com}
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I would like to request that replies to Peter Smith's question:

} We have been using a Brdu cell proliferation kit which utilises an FITC
} labelled antibody on paraformaldehyde fixed parrafin sections.We are looking
} for a counterstain so that we can view the same sections under brightfield
} conditions, particularly the nucleii. Haematoxylins seem to strip the FITC
} from the antiobody, any suggestions would be most welcome. . . .

be posted to the whole group as I am interested, too.

Also, I would be interested in a MOUNTING MEDIUM which neither fades the
fluorescence, nor the counterstain. While I have not tried this test with
fluorescence, I have tried a number of aqueous mounting media ("Mount-Quick
Aqueous", "Fluoromount G", low-temp. agarose and even "Brite" floor polish)
on stained 1 micron epoxy sections. All of the above mountants have faded
all of the counterstains which I tried (too many to list). This fading
happens before the mountant even has time to set. In past fluorescence
experiments I thought my counterstains were not working, but now I think the
10% glycerol I was using to mount the coverslips simply leached them out.

Just as an aside I have had good results using Zymed methyl green stain
(Zymed Laboratories, South San Francisco, CA, USA (800) 874-4494; no
affiliation with myself) on 1 um epoxy tissue sections. This is sold as a
counterstain for immunoperoxidase/DAB staining; I don't know its effect on
fluorescence.

TIA,

Karen Zaruba

Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
3M Company
3M Center 270-1S-01 Phone: 612-737-2971
St. Paul, MN 55144-1000

These opinions are my own and may not represent those of 3M.







From: Zhenquan Liu :      zqliu-at-pccms.pku.edu.cn
Date: Thu, 11 Jul 1996 11:11:03 -0600 (CST)
Subject: Filament life

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X-Nupop-Charset: English

We had our new filament on our Amray FEG 1910 on April 12 this year,
the extraction current ( Iext) was high (about 120 micro A) until
few days ago. The current dropped to about 55 micro A and
we have decided to stop using it untill we know the way to solve it.
(The vaccum system works well, 0.4x10-10 torr at gun)

Question:
What is the normal life of a filament such as ours?
How to make the filament life longer?
We only have our machine for a little over one year, we need
more experiences.

Thanks in advance.

Zhen Quan Liu
zqliu-at-pku.edu.cn

---------------------------------------------------------------------
Zhen Quan Liu (Ph.D) Tel:(86) 10 6275 1427(Office)
Physics Building (86) 10 6275 3727(Home)
EM Lab. Email: zqliu-at-pku.edu.cn
Physics Building Email(home) wl-at-ibmstone.pku.edu.cn
Peking University Fax (office): (86) 10 6275 1615
Beijing 100871, China




From: melliott
Date: 10 July 1996 13:53
Subject: staining

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Message-Id: {9607110407.AA28973-at-MIT.MIT.EDU}

I was asked by someone in our lab to post the following.

Mark Elliott


Recently, I have experienced two kinds of staining problems:

1. background staining &
2. peppery lead precipiate staining on cytoplasm.

The routine post-stains that we use are uranyl acetate and Sato's
lead. This method never used to give any background staining.
Occasionally we get peppery lead precipitate on cytoplasm of epon
sections, as described in textbooks.

Meanwhile, this kind of background staining is most prominent with
epon sections and not as bad with Spurr's sections. So far, the LR
White sections from human biopsies have not shown much of this
problem.

I have checked for all possible causes including staining duration,
cleanliness of boat, of washing distilled water, and of tools. If I
were to suspect some of the Epon ingredients that might have gone
out-dated, same might have happened to the Spurr's.

I have tried some standard methods described in textbooks for removing
stain contamination but they did not seem to work that well.

Can anyone offer any advice on how I can go about solving this problem
or removing background stains?

Thank you!


Fanny Chu
Pulmonary Research Lab
St. Paul's Hospital
Vancouver, BC
Canada




From: Campbell36-at-aol.com
Date: Thu, 11 Jul 1996 08:24:48 -0400
Subject: Re: Tungsten wire

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In a message dated 96-07-09 12:54:35 EDT, dusevich-at-astro.ocis.temple.edu
(Vladimir Dusevich) writes:

{ { Subj: Tungsten wire
Date: 96-07-09 12:54:35 EDT
From: dusevich-at-astro.ocis.temple.edu (Vladimir Dusevich)
To: microscopy-at-Sparc5.Microscopy.Com



Where can we buy tungsten wire of 0.012 inch diameter?
} }
Goodfellow offers a wide variety of tungsten wire in various sizes. They are
located in Berwyn, PA and the toll free number is 1-800-821-2870 or via fax
1-800-821-2020. Denton Vacuum has no financial interest in this company.

James Campbell
Marketing Manager
Denton Vacuum, Inc
609-439-9100 Fax 609-439-9111
campbell36-at-aol.com
July 11, 1996
8:19 am




From: fskarl-at-goodyear.com (Frank Karl)
Date: Thu, 11 Jul 1996 09:22:56 -0500
Subject: Two Cents on Lobbying

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This listserver (in my opinion) should not allow political comment and
discussion. While science must have a political component and awareness
this is not the place to discuss it. Where do you draw the line? If you
allow dissuasion of funding will you allow my concern about CCW on the
university campus? If I disagree a proposed topic and want to lecture
about my concerns about "non-Christian value" would you want to hear about
it?

I sort of agree with Alfred Kracher, set up your own web site and forward
one note about it. Or go one better, set up your own listserver to deal
with your agenda.


These opinions are mine alone and have no relationship to my employer.
Thank you.

Frank Karl

They that give up essential liberty to obtain a little
temporary safety deserve neither liberty nor safety.
Benjamin Franklin








From: KEVIN HALCROW :      HALCROW-at-admin1.csd.unbsj.ca
Date: Thu, 11 Jul 1996 11:48:38 ADT
Subject:

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Subscribe microscopy Halcrow-at-unbsj.ca
Dr Kevin Halcrow, Telephone (506)-658-5567
Professor of Biology, Fax (506)-658-5650
University of New Brunswick, EMail Halcrow-at-unbsj.ca
Saint John, NB
Canada
E2L 4L5





From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Thu, 11 Jul 1996 10:26:10 -0400
Subject: Re: Fluorescence-counter stain

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Message-ID: {7303E53101F70300-at-mhs.unc.edu}
In-Reply-To: {66F1E43101F70300-at-mhs.unc.edu}

} We have been using a Brdu cell proliferation kit which utilises an FITC
9+} labelled antibody on paraformaldehyde fixed parrafin sections.We are
looking
} for a counterstain so that we can view the same sections under
brightfield
} conditions, particularly the nucleii. Haematoxylins seem to strip the
FITC
} from the antiobody, any suggestions would be most welcome. . . .

I would suggest a 1% aqueous solution of Methyl Green, staining time
approx. 1 minute (or less).
It worked fine with my tissues although it is a pain to make up as the
Methyl Green has a mind of it's own as to what the powder will land on.

} Also, I would be interested in a MOUNTING MEDIUM which neither fades the
fluorescence, nor the counterstain. {

Innovex Biosciences sells Advantge Whch is a permanent aqueous mounting
media (to be used with coverslips). I have used it and it works
exceptionally well. (the usual disclaimer). Phone - (510) 222-7800

regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 7/10/96 9:34 PM
Subject:

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Microscopists, a microbiologist came by today and asked if I knew
anything about "goniometric eyepieces". He wants to determine the
hydrophobicity of bacterial colonies by measuring the contact angle of
water droplets ricocheting off the surface of the colonies using this
eyepiece on a monocular microscope. Is anyone familar with this technic
and/or knows where one can get such an eyeiece? Also, are there other
methods to determine hydrophobicity? Any help or leads would be appreciated.

Thanks, Hank Adams
EML,
NMSU
las Cruces NM THE DROUGHT IS OVER IN NEW MEXICO!! YEH





From: huffe-at-carbon.chem.nyu.edu (Edward J. Huff)
Date: Thu, 11 Jul 1996 13:45:47 -0400
Subject: Re: goniometric eyepiece

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} Applied Image Inc in Rochester, NY, (715)482-0300.
I dialed that and got a recording.
I went to http://www.bigbook.com/, entered
"Applied" in business name, "Rochester" in city,
NY in state, and found out that the correct phone number is

Applied Image Inc

Category: Electrical Fittings & Cons Material
Circuit Breakers Electric
Electrical Apparatus & Equip
Address: 1653 Main St E
Location: Rochester, NY
14609
Phone: (716) 482-0300

The display includes a street map showing how to get there.

The yellow pages categories look a little fishy...




From: kna101-at-utdallas.edu
Date: Thu, 11 Jul 1996 07:39:10 -0500 (CDT)
Subject: Re: staining

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Message-Id: {9607111716.AA21927-at-tango.qi2.com}

Fanny,

I have seen this background stain on blocks that were left in the oven
for more than a week or on blocks that were polymerized in a humid
environment. Usually, the background stains on the semithin sections
that I mount on a slide and stain with toluidine blue as well.

I don't have a remedey for the blocks that already show this though.
Just try polyimerizing the new blocks in the dessicator.

Hope this helps.
Karen P.

On Wed, 10 Jul 1996 melliott-at-prl.pulmonary.ubc.ca wrote:

} I was asked by someone in our lab to post the following.
}
} Mark Elliott
}
}
} Recently, I have experienced two kinds of staining problems:
}
} 1. background staining &
} 2. peppery lead precipiate staining on cytoplasm.
}
} The routine post-stains that we use are uranyl acetate and Sato's
} lead. This method never used to give any background staining.
} Occasionally we get peppery lead precipitate on cytoplasm of epon
} sections, as described in textbooks.
}
} Meanwhile, this kind of background staining is most prominent with
} epon sections and not as bad with Spurr's sections. So far, the LR
} White sections from human biopsies have not shown much of this
} problem.
}
} I have checked for all possible causes including staining duration,
} cleanliness of boat, of washing distilled water, and of tools. If I
} were to suspect some of the Epon ingredients that might have gone
} out-dated, same might have happened to the Spurr's.
}
} I have tried some standard methods described in textbooks for removing
} stain contamination but they did not seem to work that well.
}
} Can anyone offer any advice on how I can go about solving this problem
} or removing background stains?
}
} Thank you!
}
}
} Fanny Chu
} Pulmonary Research Lab
} St. Paul's Hospital
} Vancouver, BC
} Canada
}




From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Thu, 11 Jul 1996 12:15:53 -0600
Subject: EM and the government

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Message-Id: {v01540b08ae0af24b4a54-at-[128.206.15.200]}
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Greetings,
Frank Karl suggests no politics on the list because you can't draw
the line. But I think the line can be drawn, at least to the same degree
that the line can be drawn between microscopy topics and other techno
stuff. I wonder how many EM centers whose staff participate on this list
have had equiptment paid for by the NSF's equipment money. I bet its most
of them (at least those in the USA). The loss of this money should be of
interest to at least as many readers as are interested in, say, pepper
artifacts. Clearly the fault of the messages that set off this thread was
the multiple posting, 1 per state. But a single message about the alert
serves the interest of many on the list and should be allowed.

If some religous group decided that e.m. was immoral (imagine an
artist took a homoerotic picture that somehow involved an electron
microscope) and were fulminating to pass a law banning e.m., such a
circumstance would obviously also be appropriate political material for
this list. The usual random fulminations of religous groups omit electron
microscopy and so are obviously off limits. I don't see what the problem
with line drawing is.

Just my two, well three or four, cents,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Thu, 11 Jul 1996 09:18:27 -0500
Subject: Enough on Lobbying.....Nestor

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Message-Id: {199607111414.JAA01575-at-Sparc5.Microscopy.Com}
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Subscribers.....

I think this topic can be now laid to rest.

Pat Calarco, as member of the MSA Public Policy Committe
was told by the Society (please remember that the
Microscopy Society of America -MSA both Hosts and funds the
Microscopy Listserver) to use all it's resources (including the Listserver)
to get the information out to the membership.

In her zeal, she did go overboard, and Pat and I have touched
base and worked out a method to more succinctly get this information
out to both Society members, as well as the
microscopy community, when either might be impacted.

Certainly there are many of our subscribers who don't care about
the NSF funding for instrumentation, but there are also a significant number
that do. This is a double edged sword and there were clearly errors made
in the posting, and we have dealt with the problem. So please consider it
closed.

If anyone of our subscribers has similiar needs in their global
area (the listserver has subscribers from over 50 countries) , please touch
base
with me first. Allow me to advise you on the most appropriate method
of getting information out .

Cheers... Nestor
Your Friendly Neighborhood SysOP

P.S. All other comments on lobbying can be address to my assistant at the
address

God-at-Garden.Eden.Earth.Org











From: owenha-at-wkuvx1.wku.edu (Heather Owen)
Date: Thu, 11 Jul 1996 15:48:57 CST
Subject: Sputter Coater Instructions Needed

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Message-Id: {199607112044.PAA02282-at-Sparc5.Microscopy.Com}
MIME-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To: Microscopy-at-Sparc5.Microscopy.Com

We have an emscope SC 500 sputter coater and SB 250 carbon head,
but no instruction manuals. I have heard that this company was
aquired by another. I would appreciate any information/suggestions
on how we may be able to track down some manuals for these instruments. Thanks.

************************************************************
- -
************************************************************
Heather Owen
Department of Biology
Western Kentucky University
1 Big Red Way
Bowling Green, KY 42101-3576

(502) 745-6501 voice, (502) 745-6856 fax, Owenha-at-WKUVX1.WKU.EDU






From: huffe-at-carbon.chem.nyu.edu (Edward J. Huff)
Date: Thu, 11 Jul 1996 14:27:04 -0400
Subject: Oxygen Scavanger System

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Does anyone have detailed protocols for oxygen scavanger systems
to reduce bleaching in fluorescence microscopy?

Do I want the thymol free catalase? Do I want dry powder or=20
the crystal suspension in water? How long is the enzyme solution
good for? (The catalase assay procedure says "use these dilute
solutions promptly"). Is there any way I can make up a 100X
stock of the two enzymes and be able to keep it for a while,
like say a week?

In Nature 380: 451-453 (1996)[96180121] R. D. Vale, T. Funatsu,=20
D. W. Pierce, L. Romberg, Y. Harada & T. Yanagida,
I see "...0.5% mercaptoethanol and an oxygen scavanger system[20]..."
where note 20 is J Mol Biol 216: 49-68 (1990)[91039337] Y. Harada,=20
K. Sakurada, T. Aoki, D. D. Thomas & T. Yanagida.

In that paper, (page 51) they give only the following information:

buffer 1: 25 mM KCl, 5mM MgCl2, 20mM Hepes (pH 7.8)
buffer 2: buffer 1 + 0.5% 2-mercaptoethanol,=20
4.5 mg glucose/mL,=20
216 =B5g glucose oxidase/mL,=20
36 =B5g catalase/mL=20

I am planning to try this system, but I'm not sure about
enzyme stability, exactly which kind to use, etc.

(People here have been using 30% 2-mercaptoethanol instead,
but it has some fluorescent impurities, we don't want to
distill it, it is difficult to filter, and I hope to find
that the oxygen scavanger system gives longer exposure times
without bleaching).

I have verified that the amounts make sense: this should be
enough to eliminate 5 times saturation with oxygen. (The
enzyme system uses 2 moles of glucose to remove 1 mole oxygen).=20

I have concluded that there is not much point in using beta-D-glucose
rather than just D-glucose. The optical rotation of beta-D-glucose
changes to that of D-glucose with a half life of 48 minutes, so
to get any benefit of using the beta anomer, you would have to use
it immediately after dissolving. Also regular D-glucose is 63% beta
anyway. (Methods in Enzymology, 63:371 note a).

I want to avoid fluorescent impurities and the Sigma beta anomer=20
is only 97+% pure while the plain D glucose is 99.5% pure.

Solubility of Oxygen in water: CRC Handbook Chemistry & Physics (1987) =
pB112.=20
3.16 mL of O2 dissolves in 100 mL of water at 25=B0C.=20
(1 Atm)(3.16 mL)/((0.082LAtm/Kmol)(298K)) =3D 0.129 mmol of O2 in 100 mL =
=20
The solubility of Oxygen is 1.29 mmol/L, under 2mM.=20




From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Thu, 11 Jul 1996 09:19:10 -0500
Subject: Re: staining

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Message-Id: {199607111414.JAA12721-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} I was asked by someone in our lab to post the following.
}
} Mark Elliott
}
}
} Recently, I have experienced two kinds of staining problems:
}
} 1. background staining &
} 2. peppery lead precipiate staining on cytoplasm.
}
} Fanny Chu

I don't know about the background (will ask), but the lead pepper
could be a pH problem--check that the Pb stain is } =ph *12*, not less.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********






From: loakford-at-hsc.unt.edu (Larry Oakford)
Date: Thu, 11 Jul 1996 15:42:16 -0500
Subject: apathetic microscopists

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It is a sad commentary on how apathetic our professional community
has become when a call for action by this community from the society that
funds the operation of this listserver can elicit such a volume of negative
criticisms. No wonder we are looked upon with such disdain and cynicism by
the public. Our apathy and pettiness has seen the dismantlement of most of
the shared instrumentation programs at the federal level save those from
the Department of Defense. It is great to sit about and discuss all the
fantastic new technologies available to the microscopy community but what
will happen when the avenues to acquiring these new technologies is slammed
permanently shut for many of us. Most private sources of funding require a
commitment FIRST from the institution and other public sources before funds
are granted for such ventures.
I wonder how many of you realize that the request which was the
subject of this discussion was requested by the senate sub-committee
considering these appropriations and that previous input had been greatly
appreciated and recognized. Our silence to them and vindictive attitude
toward those trying to take a positive step toward providing these
committees with their requested input sends the wrong message to everyone.
If we are unwilling to support each other through the sharing of our
knowledge with those on this listserver as well as those outside the
listserver when requested, we shall definitely deserve the fate that awaits
us, "......a slow fade to black".
Some of the criticisms I have seen were constructive, i.e. A single
alert message rather than 6 or 7 (not 50 as has been incorrectly stated,
those went out for states where senators sit on the sub-committee making
these decisions). But divorcing solicited input required for making
INFORMED political decisions can only hurt this community. Could we all be
a little more tolerant, there are not many of these requests each year (2-3
I believe).

That's my 2 cents.
Larry

XXX
XXX
XXX
XXX
[]-XXX---
XXX
XXX
-------XXX--------
| o XOX [] |
-------XXX--------
| |
| |
------------------

Lawrence X. Oakford, Ph.D.
Department of Anatomy and Cell Biology
UNT Health Science Center
Fort Worth, TX 76107
e-mail:xavier-at-jove.acs.unt.edu






From: Dominique Miller :      dominique-at-ffaltd.demon.co.uk
Date: Thu, 11 Jul 1996 17:12:26 +0100
Subject: apathetic microscopists

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Message-ID: {vtEvPBAqfS5xEwzp-at-ffaltd.demon.co.uk}

WWW site: http://www.demon.co.uk/ffaltd/

Foster Findlay Associates Ltd is one of the leading image processing
software developers for microscopists and general medical applications.
Below is a short description of three software packages which should be
of interest to you as an active micrsocopist. These packages are
C_Images 3D (a unique library of true 3D image processing functions with
AVS/Express visualisation), PC_Image and C_Images 2D. For further
information about these products and a demo copy of the software contact
me Dominique Miller or visit our WWW site.

PC_Image 2.2 The Latest High Performance Imaging Solution From Foster
Findlay Associates Ltd.

July sees Foster Findlay Associates launch the most powerful version of
their best selling software yet - PC_Image 2.2. This comprehensive,
multi-purpose image processing and analysis solution, now offers a new,
intuitive user interface and the capacity to add your own modules. This
new facility allows you to easily integrate your own tailor-made mini-
applications into PC_Image, giving added flexibility, speed, almost
endless functionality and can now also be linked to our new microscope
stage control software - WinStage.

PC_Image 2.2 combined with our C_Images library, of over 350 functions,
is an unbeatable partnership wherever there is a need for analytical
image processing. PC_Image not only provides the ease of use and
familiarity associated with a Windows based application, but it places a
unique development package of processing and analysis functions at your
finger tips, quickly enabling you to develop full blown applications
that can be inserted directly into PC_Image. Custom applications can be
developed by us, or purchased off-the-shelf, for example, modules
already exist for grain sizing or three colour component capture.

PC_Image features calibration, LUT transforms, morphology, various
convolutions and 6 non-linear filters, FFT, thresholding, a
comprehensive selections of measurements, shade correction and
densitometry. PC_Image can process true 24 bit colour and images of
1024x1024 are easily supported, input can be from CCD cameras or a
variety of image formats such as TIFF, Windows Bitmap, Biorad, Kontron,
PGT, Seescan, SFI, Visionetrics, Data Translation and many more.
PC_Image 2.2 is now available for Windows 3.1and '95 and continues to
support an ever increasing number of framegrabbers including several PCI
boards.

C_Images 3D is a comprehensive image processing and analysis library.
Accessible at different levels, C_Images 3D is a powerful environment
for building end-user applications or implementing your own complex
algorithms. These include more accurate results and measurements, some
of which can only be obtained in 3D, such as surface area, length and
orientation of principal axes of an object. The unique capability of
C_Images 3D for handling images provides the means to process datasets
much larger than available RAM. C_Images 3D makes use of AVS, which
provides an easy to use interface with interactive modular programming
and visualisation capabilities.

For further information contact:
Mr Dominique Miller
Newcastle Technopole
Kings Manor
Newcastle upon Tyne NE1 6PA
United Kingdom
Tel: +44 (0) 191 201 2180
Fax: +44 (0) 191 201 2190
Email: Dominique-at-ffaltd.demon.co.uk

----------------------------------------------------------------------------
|From: Dominique Miller | |
|Marketing Executive | |
|Foster Findlay Associates | Phone: National (0191) 201 2180 |
|Newcastle Technopole | International +44 191 201 2180 |
|Kings Manor | Fax: National (0191) 201 2190 |
|Newcastle upon Tyne | International +44 191 201 2190 |
|NE1 6PA |E-Mail: Dominique-at-ffaltd.demon.co.uk |
|UK | WWW: http://www.demon.co.uk/ffaltd/ |
----------------------------------------------------------------------------




From: Robert Derby :      derby-at-pb.net
Date: Thu, 11 Jul 1996 19:21:23 -0500
Subject: Subscribe

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Please subscribe.





From: MicroToday-at-aol.com
Date: Fri, 12 Jul 1996 07:18:31 -0400
Subject: ElectroScan

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Just announced - Philips Electronics North America Corporation has acquired
the assets of ElectroScan. According to the press release, ElectroScan
employees will be offered employment with Philips.
Don Grimes, Microscopy Today




From: Heike Buecking :      heibueck-at-uft.uni-bremen.de
Date: Fri, 12 Jul 1996 13:54:34 +0200
Subject: SEM with X-ray microanalysis

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Message-Id: {1.5.4.32.19960712115434.00678cdc-at-alf.zfn.uni-bremen.de}
X-Sender: heibueck-at-alf.zfn.uni-bremen.de
X-Mailer: Windows Eudora Light Version 1.5.4 (32)
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Content-Transfer-Encoding: quoted-printable

Dear colleagues,

Our institute is working especially with x-ray microanalytical
investigations of nutrients (for example phosphorus, potassium) in different
plant materials. Our previous analytical unit was an Philips TEM EM 420 with
an EDAX 9100.=20
Now we are planning to buy a new SEM with a x-ray microanalytical unit. We
have seen one new equipment from ZEISS (GEMINI) with a cryo-unit, made by
Oxford Instruments and a Si/Li detector (LINK). The demonstration, we=B4ve
got, was not convincing and we are not sure, that this instrument is
suitable for our question. Who have experiences with this equipment and the
detection of light elements and who is working with other analytical units
and a similar questions.

Heike Buecking
Dr. Heike Buecking
Universitaet Bremen
Physiologische Pflanzenanatomie
UFT
Leobener Str.
28359 Bremen
FRG
Tel: 0049-421-2182954
Fax: 0049-421-2183737
e-mail: heibueck-at-uft.uni-bremen.de





From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 12 Jul 1996 09:15:27 -0500
Subject: Image translators

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I presently use Pizzaz Plus for handling images on the PC. On the Mac I use
Graphic Converter. Someone told me that Hijack and U-Pal for the PC are
comparable to the mac Graphic Converter. Anyone knows other reasonably priced
PC soft for converting formats (e.g, PICT to TIFF, etc) out there. Photoshop
is out of my price range now. Please respond to me directly not to the list
{Fermin-at-tmc.tulane.edu} . Thanks.

*********************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} ----} Prof. Pathology & Otolaryngology *
*http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html*
*********************************************************************




From: slakmon-at-soquelec.com (SOQUELEC Ltd.)
Date: Fri, 12 Jul 1996 11:35:36 -0400 (EDT)
Subject: Re: Sputter Coater Instructions Needed

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Hi,
EMSCOPE was bought by the VG Microtech (Polaron) division of Fisons a few
years ago. VG Microtech's distributor in U.S.A. is Energy Beam Sciences.
Give them a call at 413-786-9322 (Steve Slap or Jeff Balou) or email at
ebs-at-ebsciences.com. I am sure they can assist you.

Good luck

Jean-Pierre Slakmon

} We have an emscope SC 500 sputter coater and SB 250 carbon head,
} but no instruction manuals. I have heard that this company was
} aquired by another. I would appreciate any information/suggestions
} on how we may be able to track down some manuals for these instruments.
Thanks.
}
} ************************************************************
} - -
} ************************************************************
} Heather Owen
} Department of Biology
} Western Kentucky University
} 1 Big Red Way
} Bowling Green, KY 42101-3576
}
} (502) 745-6501 voice, (502) 745-6856 fax, Owenha-at-WKUVX1.WKU.EDU
}
}
}
}
________________________________________________________________

Jean-Pierre Slakmon, Eng. Tel: (514) 482-6427
SOQUELEC Ltd. Fax: (514) 482-1929
5757 Cavendish Blvd., Suite 101
Montreal, Quebec e-mail: slakmon-at-soquelec.com
H4W 2W8, Canada http://www.soquelec.com
________________________________________________________________





From: Dave King (607)757-1248 T37/257-3 Ext DEKING-at-VNET.IBM.COM
Date: 12 Jul 1996 11:39:51 EDT
Subject: Add SONY 890 to SEM?

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Message-Id: {199607121555.KAA03824-at-Sparc5.Microscopy.Com}

To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}
cc: VANHART --ENDVM5 Dan VanHart 7-1262 EMMI --ENDVM5 F. Emmi
SHURBAN --ENDVM1 Hurban, S. CONROW --ENDVM5 K.M. (Karen) Conro
JUNGDY --ENDVM5 Dae-Young Jung RICKM --ENDVM1 Musa, R.


We've evaluated video thermal printing on several of our SEM's
as an inexpensive replacement for instant film. It's OK for low
resolution needs, as long as the image is not very noisy. Our
Cambridge S-250 has an image store, so I can beat the noise problem
in that case. Our Phillips can not "build" a video image, so the use
of thermal prints is less.

We'd like to implement a SONY 890 or similar video printer on an
AMRAY 1600 a 1610 and a JEOL 733 probe. My impression is that the
cost of the hardware and software needed to interface to these
would wipe out years of savings. I heard the Amray TV cards are
even out of production.

Ideas? Thanks.

. {
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: Emeylan-at-aol.com
Date: Fri, 12 Jul 1996 20:30:04 -0400
Subject: Re: LM - Need parts for Zeiss scope

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Dear Ed,

I have a service company for microscopes. My stock is over $250 000.00 in
parts and accessories for any type of microscope.

If I would want to make a parts and price list, I would need two persons
wotking full time on it, because this come and goes, and it never comes twice
with the same cost.

I recommend you let me know specifically, what you need, and I will look if i
can help you.

best regards, Emile Meylan




From: Yeong Seok Kim :      iecco-at-nuri.net
Date: Sat, 13 Jul 1996 12:17:38 +0900
Subject: Microscopy & Microanalysis in Minneapolis

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Message-ID: {31E71552.523-at-nuri.net}

Hi exhibitors,

If there are anyone among the exhibitors who will take part in
Microscopy & Microanalysis annual meeting, please contact me. I would
like to make a business talk before going there. Our company will be
able to be your business partner in Korea.

Thanks in advance.

My e-mail address is iecco-at-nuri.net.




From: Probing & Structure :      pns-at-ultra.net.au
Date: Sat, 13 Jul 1996 18:38:50 +1000
Subject: Re: SEM with X-ray microanalysis

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At 13:54 12-07-96 +0200, you wrote:
} Dear colleagues,
}
} Our institute is working especially with x-ray microanalytical
} investigations of nutrients (for example phosphorus, potassium) in=
different
} plant materials. Our previous analytical unit was an Philips TEM EM 420=
with
} an EDAX 9100.=20
} Now we are planning to buy a new SEM with a x-ray microanalytical unit. We
} have seen one new equipment from ZEISS (GEMINI) with a cryo-unit, made by
} Oxford Instruments and a Si/Li detector (LINK). The demonstration, we=B4ve
} got, was not convincing and we are not sure, that this instrument is
} suitable for our question. Who have experiences with this equipment and the
} detection of light elements and who is working with other analytical units
} and a similar questions.
}
} Heike Buecking
} Dr. Heike Buecking
} Universitaet Bremen
} Physiologische Pflanzenanatomie
} UFT
} Leobener Str.
} 28359 Bremen
} FRG
} Tel: 0049-421-2182954
} Fax: 0049-421-2183737
} e-mail: heibueck-at-uft.uni-bremen.de
****************************************
Dear Heike:
The largest difference between thick (SEM/Microprobe) and thin sections
(TEM) EDS analysis is the size of the "penetration envelope". That is the
depths and the area from which the X-rays originate. In thin sections that
area is effectively the beam diameter. =20

In sections too thick for beam penetration (SEM) the area from which X-rays
originate, with the instrument at a very high magnification, depends mostly
on kV and the average atomic number of the specimen. For average rock that
may be around 3 microns diameter. For biological materials this tends to be
around 20 microns. You could certainly analyse those elements, but you
could not tell, for most biologist's purposes the location of higher
concentrations.=20

Furthermore, if the higher concentrations ocurred in, for instance, part of
chloroplasts at 5%, this would result in a sizeable peak. The accross the
cell percentage may be only 0.2% and perhaps not detectable in EDS.=20

There are always exceptions, but EDS on TEM is more useful to biologists and
EDS on SEM is generally more useful for the material scientist.
Es ist wirklich ganz einfach.
} Jim Darley

}
Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site http://www.ultra.net.au/~pns/
***********************





From: Emeylan-at-aol.com
Date: Sat, 13 Jul 1996 17:10:54 -0400
Subject: Re: Microscope parts

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In a message dated 96-07-12 23:41:39 EDT, you write:

{ { They use a Nachet optics at this time. Can you assist? } }

I am not very familiar with Nachet optic. I deal mainly with Carl Zeiss,
Leica, Nikon, Olympus. But a trinocular head could always be adapted if
necessary.

Do you have more optical information about Nachet optic ?

Binocular, trinocular have to be adapted to the particulary optic. It is
either infinity corrected or a particular focal distance. We need to know,
what this focal distance is.

If you need more help, let me know,


Emile Meylan






From: Emeylan-at-aol.com
Date: Sat, 13 Jul 1996 17:10:41 -0400
Subject: Re: No Subject

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In a message dated 96-07-13 00:58:26 EDT, you write:

{ { Do you also sell used microscopes and other equipment?
Right now I am looking for a used stereomicroscope, with zoom (or at least
covering a wide range of magnifications, like x5-25), no cameraports
needed, a "boomstand" would be a great plus. { {


Dear Laszlo,

I mainly service and repair microscopes. But good service does not have
limit. Selling is also a service. I do help and sell sometimes
microscopes.
I actually serviced several microscopes at your location.

Reference: Tony Piazza 415/750-2167 Bldg2 room 436B

I can help you find a stereo microscope, with zoom on a boom stand.

} } Also, could you give some insigth about really cheap I mean low priced
stereomicroscopes? I have seen several supply companies selling
stereomicroscopes from Korea and from Russia for about $500. Are they any
good? } }

Microscopes are like anything else. Cars, for example.
You can buy a car for $500 and another for $100 000.
You cannot expect from the $500 car the same result as from the other one !

If you were sombody buying a microscope to use it twice a year for ten
minutes, I would sell you a $500 microscope.

However, if you think to use this microscope for routine work altmost every
day, I would think, that a better and more expensive microscope might be a
good investment.

Let me know, what do you think of it.

best regards, Emile Meylan
SERCO Technical Services, Inc.
800/483-0508









From: Emeylan-at-aol.com
Date: Sat, 13 Jul 1996 17:08:54 -0400
Subject: Re: LM - Need parts for Zeiss scope

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In a message dated 96-07-13 10:14:54 EDT, you write:

{ { Are you involved exclusively with optical scopes, or do you service SEM's
as well? If you service SEM's, I'd like to know more about your company.
} }

Dear John,

I am only involved with Light microscopes. To service SEM's you would need
the 100% support of the vendor of this microscope. Making my own company,
I do also compete with this vendors, and some of them do not like it very
much.

However, I have been working with the Company Carl Zeiss for many years.
I still have a very good and very close relationship with them. If your
service or parts problem is related to Carl Zeiss, let me know. I could
maybe help you with the right contact.

with best regards,

Emile Meylan
SERCO Technical Services, Inc.




From: Gregory Forbes :      gaf2-at-cornell.edu
Date: Sat, 13 Jul 1996 23:06:43 -0400 (EDT)
Subject: resin for embedding plant tissue

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I would be grateful if someone could suggest a suitable embedding medium
(preferably alternatives to paraffin) to section plant leaf tissue for light
microscopy.

Thank you.

Greg Forbes
Greg Forbes, plant pathologist, International Potato Center (CIP)
Quito, Ecuador

Email: gaf2-at-cornell.edu

CURRENTLY: Dept. Plant Pathology, Cornell University,
334 Plant Science Bldg. Ithaca, NY 14853
Fax: +1(607)2554471 Tel: +1(607)2553188





From: Yathika Perera :      yperera-at-mail.coin.missouri.edu
Date: Sun, 14 Jul 1996 09:30:50 -0500 (CDT)
Subject: unsubscribe

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unsubscribe




From: marilyn-at-cemmsa.adelaide.edu.au (Marilyn Henderson)
Date: Mon, 15 Jul 1996 09:44:23 +0900
Subject: Unsubscribe

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Message-Id: {199607142335.XAA24626-at-traminer.cemmsa.adelaide.edu.au}
X-Sender: marilyn-at-traminer.cemmsa.adelaide.edu.au
Mime-Version: 1.0
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Please

UNSUBSCRIBE 'marilyn-at-cemmsa.adelaide.edu.au (Marilyn Henderson)'

I've already tried by the advised method and am still getting messages.

Thanks






From: em-at-mediacity.com (Ed Monberg)
Date: Sun, 14 Jul 1996 15:12:22 -0800
Subject: CRASSLY COMMERCIAL PURCHASE OPPORTUNITY Amray & Cambridge

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Message-Id: {v01540a19ae0dba0293e4-at-[205.216.172.10]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear fellow list members,

A client has several units in a warehouse,
out of service, complete, but offered "as is".
Not items we would normally buy and warehouse, though.

Any fellow listmember is invited to please
send your best bid, remembering that they are "as is" units,
though guaranteed complete, and I will present it to the owner.


1 AMRAY SEM 1500

- - with a handler for 6" wafers
(although it is not necessary to use it)

2 CAMBRIDGE STERIOSCAN 240






Regards,



(signed) Ed Monberg {em-at-mediacity.com}

--------------------------------------------------

510-429-1060 Fax 429-1065
LMDC, (Laser & Motion Development Co.)
3101 Whipple Road
Union City, CA 94587-1216

FOR A TEXT CATALOGUE:
For our Most recent Catalogue of "On Hand" EQUIPMENT:
Send empty mail to: {Cat-at-lasermotion.com}


Our web page: http://www.lasermotion.com (Is beginning to take shape!)
Our e-mail: office-at-lasermotion.com






From: manuela :      manuelap-at-petermac.unimelb.edu.au
Date: Mon, 15 Jul 1996 14:06:48 +1000
Subject: LM paraffin embedding

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I would like to address a specific problem we have encountered. We have been
taking mouse eyes through to wax and have been having trouble keeping the
vitreous intact. Results have been inconsistant - sometimes the vitreous
cuts beautifully and others not. We have used a range of protocols varying
dehydration and infiltration times as well as using toluene as the clearing
agent (we routinely use xylene). Has anyone had experience cutting eyes?
Any suggestions will be welcomed.

Thanks in advanced

M.Palatsides





From: Simone Graber t4534 :      Simone.Graber-at-biologie.uni-regensburg.de
Date: Mon, 15 Jul 1996 10:14:38 +0200
Subject: unsubscribe

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please unsubscribe

simone.graber-at-biologie.uni-regensburg.de




From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Mon, 15 Jul 1996 8:47:37 -0400
Subject: Re: microtome manual

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Message-Id: {199607150741.DAA05924-at-thomas.ge.com}
X-Authentication-Warning: thomas.ge.com: Host [3.52.8.39] didn't use HELO protocol

John,
Reicher-Jung is now a part of Leica ph. 800-248-0123. They should have a
copy they can send you.
regards,

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: Dr. A. J. Garratt-Reed :      t.g_reed-at-fs2.mt.umist.ac.uk
Date: Mon, 15 Jul 1996 15:38:37 +0001
Subject: NSF Important Notice 91

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Comments: Authenticated sender is {t.g_reed-at-fs2.mt.umist.ac.uk}

In the past there have been discussions here concerning (or making
reference to) the NSF's Important Notice 91, concerning use of
NSF-sponsored instrumentation by Universities for a fee. I have not
been able to find an on-line copy of this document, so I have had it
transcribed and made it available at
http://18.82.0.42/nsf.in91/in91.html, subject to the understanding
that neither MITor myself are liable for any transcription errors.

Tony Garratt-Reed

*******************************
** **
** Anthony J. Garratt-Reed **
** tonygr-at-mit.edu **
** **
*******************************




From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Mon, 15 Jul 1996 09:18:34 -0500
Subject: Re: Staining Request

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Message-Id: {199607151413.JAA11415-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Bob,
I don't have a stain right to hand, but (assuming you mean LM
stains) I suggest you get the following books for your library:
"Conn's Biological Stains", 9th edition or later--chemistry,
reactions, and properties of stains.
"Staining Procedures", 4th edition or later. Recipes.
both by the Biological Stain Commission, published by Williams & Wilkens
(Baltimore)
Kiernan, J.A."Histological & Histochemical Methods: Theory &
Practice." 1990, 2nd edition (may be a later one). Pergamon (NY). Excellent
reference/text. Info you want is on pp. 288-298 for amines. Other pages for
other "amine-like" groups. (Keirnan may reply himself to your question;
this would be most useful.)
Phil

} Fellow Microscopists;
}
} Once again I am in need of a specific stain, and being unfamiliar with
} stains in general, I ask your assistance. I am looking for a stain that is
} specific to a compound which is cationic, w/amine-type functional groups.
} I would like to be able to visualize the homogeneity of a coating on a
} substrate, (preferably by light microscopy, but we do have SEM and EDX).
} Someone recommended the use of TNBS (trinitrobenzenesulfonic acid, or
} picrylsulfonic acid), but I would like to think that there is something a
} little less hazardous to use (as well as a little less expensive to
} obtain).
}
} TIA,
}
} Bob
} *******************************
} Bob Citron
} Chiron Vision
} 555 W. Arrow Hwy
} Claremont, CA 91711
} USA
} ph: (909)399-1311
} email: Bob_Citron-at-cc.chiron.com
} ********************************

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********






From: petralia-at-pop.nidcd.nih.gov (Ronald Petralia)
Date: Mon, 15 Jul 1996 11:58:04 -0500
Subject: perfusion temperature

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Message-Id: {ae1022040002100426e6-at-[165.112.170.4]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I have heard differing opinions as to what temperature to set buffer washes
and fixatives for animal perfusion. We prepare brain tissue for
immunocytochemical localization of membrane proteins. When we perfuse rats,
we wash out the blood with room temperature phosphate buffer, and then use
cold fixative (4% paraformaldehyde in phosphate buffer with or without 0.1%
glutaraldehyde). I am interested in anyone's opinion on the best
temperature for these solutions as well as the logic behind the choice.

A related question: the phosphate buffer we use for perfusion is 0.12 M.
Does anyone wish to share their opinions on the best molarity for good
ultrastructure?

Ron Petralia

Ronald S. Petralia
NIDCD/NIH
36/5D08
36 CONVENT DR MSC 4162
BETHESDA MD 20892-4162
Tel: 301-496-3804
Fax: 301-480-3242
petralia-at-pop.nidcd.nih.gov






From: Robert Fisher :      rmfisher-at-u.washington.edu
Date: Mon, 15 Jul 1996 09:27:33 -0700 (PDT)
Subject: EDS repair

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Colleagues - We have a Link System EDS model
6111 detector with a blown window - probable
crystal damage.

We have made 1 contact but purchasing requires
several bids.

Anyone out there with names of possible repair
companies?

Please reply directly to spare the busy electrons
- it is not hard to do.

Many thanks

Bob Fisher





From: Diana L Kittleson :      Diana_L_Kittleson-at-PBTC.com
Date: 15 Jul 96 10:02:31
Subject: Epoxy Shrinkage

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Message-Id: {9607151524.AA1203-at-worldcom-37.worldcom.com}
To: Microscopy {Microscopy-at-Sparc5.Microscopy.Com}

I am casting the interior of a cylinder with an epoxy. The epoxy I am using
cures in 8 hours at room temperature and reaches a peak temperature of 82F.
The manufacturer quotes the shrinkage of this epoxy as .001in./in. I have the
following questions regarding shrinkage:
Does epoxy shrink the same in all directions (x&y vs z)?
Is there a good method for measuring shrinkage?
Would shrinkage alter the dimensions of a flexible material (draw in the sides
of my cylinder)?
Any references on this subject would be most appreciated.
Thank you for your help,

Diana Kittleson
Pillsbury Technology Center
330 University Avenue S.E.
Mpls., Mn 55414
612-330-1898
Diana_L_Kittleson-at-pbtc.com




From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Mon, 15 Jul 1996 20:26:37 +0100 (BST)
Subject: Re: resin for embedding plant tissue

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We use the acrylic resin LR White for a lot of our light microscopy work
of plant materials. It is a forgiving resin and you only need to
dehydrate to 80-90% EtOH. Iy sections very well at2-5um. Some of the
stains and histochemical agents don't work in quite the same way ie
phloroglucinol for lignin but it does give good infiltration and very
nice thin sections

Patrick Echlin
University of Cambridge UKOn Sat, 13 Jul 1996, Gregory Forbes wrote:

} I would be grateful if someone could suggest a suitable embedding medium
} (preferably alternatives to paraffin) to section plant leaf tissue for light
} microscopy.
}
} Thank you.
}
} Greg Forbes
} Greg Forbes, plant pathologist, International Potato Center (CIP)
} Quito, Ecuador
}
} Email: gaf2-at-cornell.edu
}
} CURRENTLY: Dept. Plant Pathology, Cornell University,
} 334 Plant Science Bldg. Ithaca, NY 14853
} Fax: +1(607)2554471 Tel: +1(607)2553188
}
}




From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Mon, 15 Jul 1996 20:26:37 +0100 (BST)
Subject: Re: resin for embedding plant tissue

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We use the acrylic resin LR White for a lot of our light microscopy work
of plant materials. It is a forgiving resin and you only need to
dehydrate to 80-90% EtOH. Iy sections very well at2-5um. Some of the
stains and histochemical agents don't work in quite the same way ie
phloroglucinol for lignin but it does give good infiltration and very
nice thin sections

Patrick Echlin
University of Cambridge UKOn Sat, 13 Jul 1996, Gregory Forbes wrote:

} I would be grateful if someone could suggest a suitable embedding medium
} (preferably alternatives to paraffin) to section plant leaf tissue for light
} microscopy.
}
} Thank you.
}
} Greg Forbes
} Greg Forbes, plant pathologist, International Potato Center (CIP)
} Quito, Ecuador
}
} Email: gaf2-at-cornell.edu
}
} CURRENTLY: Dept. Plant Pathology, Cornell University,
} 334 Plant Science Bldg. Ithaca, NY 14853
} Fax: +1(607)2554471 Tel: +1(607)2553188
}
}




From: VayTek, Inc. :      vaytek-at-netins.net
Date: Mon, 15 Jul 1996 15:35:22 -0500
Subject: Deconvolution

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Recently Dave Beebe wrote:

} OK, so confocal and deconvolution methodologies have their place
} for achieving the ultimate in resolution when imaging certain kinds of
specimens.
} But what about in real time on an everyday basis when you are sitting
} at the 'scope scanning through a new specimen just trying to get a sense of
what's
} going on. Is deconvolution useful for these situations, or must I wait for the
} computer to digest and regurgitate my image before I know whether I have
} something interesting? In more direct terms, is deconvolution practical
for rapidly
.} and easily evaluating new specimens where the major purpose is getting
} few informative pix, not the ultimate in 3D resolution?

and Aryeh Weiss wrote:

} I think it would be useful if users of wide field deconvolution
} systems post some processing times for "typical" dataset,
} specifying the computer configuration (hardware and software)
} which they use

At the risk of restarting the deconvolution thread again, I would like to
respond to these two points.

VayTek's goal from the beginning has been to develop a practical
deconvolution "toolbox". The toolbox approach means a complete
integrated system with several algorithms, each appropriate for
various situations and applications.

We know it is important to have rapid feedback in real-time situations.
To do this with deconvolution means integrating the data acquisition
system with a rapid deconvolution algorithm. We have done this on
both the Macintosh and the PC.

With our system, the user can easily move the stage, view a live image,
capture a single image, and apply a single image nearest neighbor
deconvolution. This process takes about 5 to 10 seconds. Color images
(which cannot be captured on a confocal system) take a few seconds longer.

If the user wants a slightly better deconvolved image, he/she can use
a nearest neighbor algorithm with three consecutive optical slices.
It takes only slightly longer to capture the three images.

There will be objections by others about the value of nearest
neighbor vs. constrained iterative and that this approach is not valid.
(Or perhaps they have a vested interest in pushing the constrained
iterative.) However, we know from experience, (many of our users will
verify this), that usually there are small difference in images deconvolved
with nearest neighbor and constrained iterative. The nearest neighbor
algorithm is very useful for determining what the image will look like
when deconvolved with the constrained iterative - and doing it
quickly.

After a specimen has been previewed with the nearest neighbor and
the proper settings have been determined, a full stack can be captured
and deconvolved with the constrained iterative.

In other words, the answer is yes, there is a way to use deconvolution
in a practical, real time situation. It may not be as fast as the confocal,
but it is pretty close. There are trade-offs however, one being flexibility.

Our integrated system does make a good "first line" system for some
researchers with some specimens.

Regarding typical times:

This a little more complex since there are several variables involved. But
I'll try.

In general, the times for Macintosh and PC are pretty comparable. All these
times are for VayTek's imaging systems.

1) 512 x 512 grayscale image with a DSP board - never more than 4
seconds/image using nearest neighbor - any size Psf

2) 512 x 512 grayscale image without DSP board - depends on CPU speed - for
Pentium 150 - no DSP board - 2 to 10 seconds - nearest neighbor - with a
small PSF; longer with large PSF

3) 512 x 512 color image with DSP board - 16 Mb memory - nearest neighbor -
10 to 15 seconds

4) stack of 25 512 x 512 grayscale images - with DSP board - sufficient
memory - small PSF - optimal number of iterations - constrained iterative -
5 to 15 minutes

5) Data acquisition - live image - real time; capture and store a single
512 x 512 image to disk - 1 to 2 seconds

6) Time to capture a single image and deconvolve using a single image
nearest neighbor algorithm - 5 to 10 seconds

7) Time to capture a stack of 25 images - 512 x 512 grayscale and write to
disk - 25 to 30 seconds

8 ) Time to deconvolve a stack of 25 images 512 x 512 grayscale using
nearest neighbor and DSP board - about 2 minutes

I hope this answers your questions


John Kesterson, Ph.D.
VayTek, Inc.
http://www.vaytek.com









From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Mon, 15 Jul 1996 16:28:23 -0500
Subject: (P)EELS mapping, USA

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We are looking for a TEM with (P)EELS mapping capability to study initiation of
calcification if soft biological tissues. We need to detect calcium and map its
distribution. EDS analysis does not seem to be feasible for us.

If you have or know of a lab in the continental USA that has this capability
please get in direct contact with:

Barbara Ellingorth, (612)481-7562 St. Jude Medical, St. Paul, MN





From: Emeylan-at-aol.com
Date: Mon, 15 Jul 1996 18:30:04 -0400
Subject: Re: LM - Need parts for Zeiss scope

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In a message dated 96-07-15 15:58:06 EDT, you write:

{ { Where are you located?
Maybe I can offer you some help. } }


Thanks for your reply. Actually, the person who needs help with SEM service
is John Best.

His email address is : jbest-at-vicon.net

Unfortunately, my email to him is always rejected by the internet.
I hope you get this one.

best regards, Emile Meylan




From: Greg2NJ-at-aol.com
Date: Mon, 15 Jul 1996 21:01:08 -0400
Subject: Looking for Service Person

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Looking for qualified service person to install, set up and maintain an
Amray 1000 SEM in the Kansas City Area Please contact Pete at
908-671-5759.




From: Greg2NJ-at-aol.com
Date: Mon, 15 Jul 1996 21:01:08 -0400
Subject: Looking for Service Person

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Looking for qualified service person to install, set up and maintain an
Amray 1000 SEM in the Kansas City Area Please contact Pete at
908-671-5759.




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: 15 Jul 96
Subject: Staining of functional groups

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-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #3.0 ] --

Bob Citron wrote the following:
==================================================
I am looking for a stain that is specific to a compound which is
cationic, w/amine-type functional groups. I would like to be able to
visualize the homogeneity of a coating on a substrate, (preferably by
light microscopy, but we do have SEM and EDX). Someone recommended the
use of TNBS (trinitrobenzenesulfonic acid, or picrylsulfonic acid), but
I would like to think that there is something a little less
hazardous to use (as well as a little less expensive to obtain).
=============================================================
You would have to be a bit more specific as to the functionality of the
"amine type functional groups". Are you talking about a good old
fashioned "quat" (e.g. "Gaf-quat")? If you are talking about substrates
like human hair or skin, which are the only substrates I have ever heard
of quats being applied, generally the amount of coating, when
realistically applied, there does not seem to be enough there to give
any kind of "effect". We have only been successful in visualizing quats
either by "Before" vs. "After" microscopy (SEM) on the same identical
area (directly on hair or using replicas if on skin). And then it is
not the coating itself but the effect of the coating that is being
resolved. In some instances, you can see the deposition in cross-
section TEM. These techniques were published in the early 1970's in the
J. Society of Cosmet. Chemists. I don't have the exact references at my
finger tips but I am sure you could find them. But the first work on
hair was authored by DiBianca and the work on skin, by the undersigned.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.
com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.
com


Look for us!
############################
WWW: http://www.2spi.com
############################
=====================================================




From: Li :      yli6-at-facstaff.wisc.edu
Date: Tue, 9 Jul 1996 21:48:15 -0500
Subject: Re: Horseraddish peroxidase/DAB

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Message-Id: {199607160534.AAA53965-at-audumla.students.wisc.edu}



----------
} From: Donald Lovett {lovett-at-trenton.edu}
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: Horseraddish peroxidase/DAB
} Date: Wednesday, July 03, 1996 3:13 PM
}
}
} I am about to begin a study of whether certain intercellular spaces are
} continuous with other spaces in crabs. All of my references suggest
} using horseraddish peroxidase with DAB and H202, but they are 20+ years
} old. Are there any more recent modifications of this technique? How
} does this compare to Lanthanum or Ruthenium? Has anyone tried an
} organism with hemocyannin? I would greatly appreciate any advice or
updates.
}
} Thank you.
}
} ______________________________________________________________________
} Donald L. Lovett e-mail: lovett-at-trenton.edu
} Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} Trenton State College, NJ 08650-4700 fax: (609) 771-2674
}




From: a1quinte-at-attila.stevens-tech.edu
Date: Tue, 16 Jul 1996 10:06:05 -0400 (EDT)
Subject: UNSUBSCRIBE

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unsubscribe





From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Tue, 16 Jul 1996 09:01:38 -0500
Subject: AFM listserver

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Message-Id: {199607161356.IAA17379-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I am on the Digital Instruments' scanned probe microscopy
listserver, but is there an AFM listserver? Thanks.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********






From: Philip Koeck :      Philip.Koeck-at-csb.ki.se
Date: Tue, 16 Jul 1996 16:00:29 +0200
Subject: Eikonix scanner

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Posted-Date: Tue, 16 Jul 1996 16:00:29 +0200
Message-Id: {31EBA07D.7030-at-csb.ki.se}

Can anybody answer some technical questions about an Eikonix scanner.

Specifically: How does the calibration work?
With our scanner it doesn't seem to make any difference how You run
the CALIB program, with lights first on then off or vice versa,
or on (off) both times.

Does anybody know whom to call for technical support?
The company in Bedford MA doesn't exist anymore.

Philip
--
Philip Koeck
Karolinska Institutet
Dept. of Bioscience
Novum
S-14157 Huddinge
Sweden
Tel.: +46-8-608 91 93
Fax.: +46-8-608 92 90
Email: Philip.Koeck-at-csb.ki.se




From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Tue, 16 Jul 1996 10:13:21 -0500
Subject: Re: Epoxy Shrinkage

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Message-Id: {199607161513.KAA14550-at-mailhub.iastate.edu}
X-Sender: wes-at-pop.ameslab.gov
X-Mailer: Windows Eudora Pro Version 2.1.2
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Sounds like your shrinkage is rather small, 1/10th of 1%. That could very
well affect the dimensions of your sample. But it is negligible compared to
the precision of most measurements that I am familiar with. You probably get
bigger errors elsewhere. A one pixel error measuring a feature with a 100
pixel diameter in a 1024 pixel-wide image will give you 1% error.

The shrinkage would ideally be the same in all directions, but can vary
depending on what kind of constraints your samplke is under. We use 1"
bakelite rings to embed samples and pour in about a 1/4" of epoxy. The rings
don't move much and because the epoxy is thin in the vertical direction and
because the top is a free surface, all the shrinkage (which isn't much)
takes place in the vertical direction. (Some of my engineering mechanics
background coming through here.) Like I said, it depends on your mold.

At 10:02 AM 7/15/96 +0000, you wrote:
} I am casting the interior of a cylinder with an epoxy. The epoxy I am using
} cures in 8 hours at room temperature and reaches a peak temperature of 82F.
} The manufacturer quotes the shrinkage of this epoxy as .001in./in. I have
the
} following questions regarding shrinkage:
} Does epoxy shrink the same in all directions (x&y vs z)?
} Is there a good method for measuring shrinkage?
} Would shrinkage alter the dimensions of a flexible material (draw in the sides
} of my cylinder)?
} Any references on this subject would be most appreciated.
} Thank you for your help,
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Emeylan-at-aol.com
Date: 07/13/96
Subject: used microscopes

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Dear Laszlo,
I replied to you 7/13/96, but got my email back from the internet. Could
not find your email address. I copy the microscopy forum in case you dont
get this one.
Emile

Subj: Re: No Subject

In a message dated 96-07-13 00:58:26 EDT, you write:

{ { Do you also sell used microscopes and other equipment?
Right now I am looking for a used stereomicroscope, with zoom (or at least
covering a wide range of magnifications, like x5-25), no cameraports
needed, a "boomstand" would be a great plus. { {


Dear Laszlo,

I mainly service and repair microscopes. But good service does not have
limit. Selling is also a service. I do help and sell sometimes
microscopes.
I actually serviced several microscopes at your location.

Reference: Tony Piazza 415/750-2167 Bldg2 room 436B

I can help you find a stereo microscope, with zoom on a boom stand.

} } Also, could you give some insigth about really cheap I mean low priced
stereomicroscopes? I have seen several supply companies selling
stereomicroscopes from Korea and from Russia for about $500. Are they any
good? } }

Microscopes are like anything else. Cars, for example.
You can buy a car for $500 and another for $100 000.
You cannot expect from the $500 car the same result as from the other one !

If you were sombody buying a microscope to use it twice a year for ten
minutes, I would sell you a $500 microscope.

However, if you think to use this microscope for routine work altmost every
day, I would think, that a better and more expensive microscope might be a
good investment.

Let me know, what do you think of it.

best regards, Emile Meylan
SERCO Technical Services, Inc.
800/483-0508




From: Emeylan-at-aol.com
Date: Tue, 16 Jul 1996 15:34:50 -0400
Subject: Re: LM - Need parts for Zeiss scope

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In a message dated 96-07-16 09:22:11 EDT, you write:

{ { A reader wants to sell some equipment including a
Zeiss oil immersion objective. Your guidance is appreciated.
} }

Dear Elinor,

Ask your customer for a little more details. Or have him contact me. A
Carl Zeiss immersion objective is very vague. It could be an objective of
$300 or $4500.

Very often, the Carl Zeiss objectives have the part nr. written on iy.

Let me know, if I can help,

Best regards,

Emile








From: Donald Lovett :      lovett-at-trenton.edu
Date: Tue, 16 Jul 1996 17:17:08 -0400 (EDT)
Subject: Wanting to Purchase Used TEM

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Last fall I posted a message indicating my interest in purchasing a used
Hitachi 600 TEM. The scope I had hoped to purchase was taken by another
group before we could get funds approved. Please respond directly to me
if you know of anyone wishing to sell their TEM. I really do not want
other brands.

Thank you.

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-trenton.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
Trenton State College, NJ 08650-4700 fax: (609) 771-2674






From: Emeylan-at-aol.com
Date: 07/13/96
Subject: Zeiss parts

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Dear John,
I replied your message on 7/13/96

The Internet did not accept your email address. I send a copy to the
microscope forum, in case you do not get this one.
best regards, Emile


Subj: Re: LM - Need parts for Zeiss scope

In a message dated 96-07-13 10:14:54 EDT, you write:

{ { Are you involved exclusively with optical scopes, or do you service SEM's
as well? If you service SEM's, I'd like to know more about your company.
} }

Dear John,

I am only involved with Light microscopes. To service SEM's you would need
the 100% support of the vendor of this microscope. Making my own company,
I do also compete with this vendors, and some of them do not like it very
much.

However, I have been working with the Company Carl Zeiss for many years.
I still have a very good and very close relationship with them. If your
service or parts problem is related to Carl Zeiss, let me know. I could
maybe help you with the right contact.

with best regards,

Emile Meylan
SERCO Technical Services, Inc.




From: wong-at-msg.ucsf.edu (Mei Lie Wong)
Date: Tue, 16 Jul 1996 17:40:28 -0700
Subject: Formvar

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The question has come up here at my facility as to whether anyone knows if
there is anything in the literature or if anyone knows - if anything has
been done to or with crosslinking formvar.
Replies can be sent to me at the above address and if there is anything I
will make a summary for the bulletin board. thank you in advance. Mei
Lie Wong

Mei Lie Wong
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
email wong-at-msg.ucsf.edu






From: Joe D Geller :      geller-at-world.std.com
Date: Wed, 17 Jul 1996 08:31:38 -0400 (EDT)
Subject: Stray Magnetic Fields

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During the installation of a new instrument we had 60 cycle magnetic field
problems. This was especially bad at the longer working distances.

The source turned out to be the electronics control unit of a cold cathode
discharge (Penning) guage (Varian) which was located right next to the
electron
optical column.

Needless to say, we now operate the instrument with the guage off.

Joe Geller
Geller MicroAnalytical Lab
426e Boston St.
Topsfield, MA 01983-1216
508 887-7000





From: RobertCO2-at-aol.com
Date: Wed, 17 Jul 1996 12:10:05 -0400
Subject: Need a SEM/EDS Video

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I consult to a place that does SEM for forensis and failure analyses. We are
looking for an educational video on SEM and EDS that we can show visiting
clients and use during technical demonstrations and presentations.

Does anyone have, know, or can sell a video along these lines. The audience
will be for the most part college educated but in non-technical subjects.
Videos from manufacturers are acceptable.

Thanks and please answer to email address: "robertco2-at-aol.com"

Robert Sherman




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Wed, 17 Jul 1996 15:21:18 -0500
Subject: fluorescent bone specimens

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I have a couple of clients who want to coverslip some ground bone
specimens. The bones were labeled in vivo with fluorescent compounds that
incorporate into the matrix. During the specimen prep, the alcohol fixed
bones are washed in xylene or acetone. Is there any reason not to use
Permount to coverslip? In addition, if anyone has any experience looking
at oxytetracycline, alizarin or calcein blue labeled bones and is willing
to talk about it, I would appreciate if they sent me a direct reply and I
will contact them. Thanks in advance.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Wed, 17 Jul 1996 15:46:54 -0400 (EDT)
Subject: Re: Need a SEM/EDS Video

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The Education Committee of MSA has a large collection of videos on
microscopy. Among the videos are two fine videos on EDS by Dale Newberry.
Information on the videos is part of the MSA website at
www.msa.microscopy.com.

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************

On Wed, 17 Jul 1996 RobertCO2-at-aol.com wrote:

} I consult to a place that does SEM for forensis and failure analyses. We are
} looking for an educational video on SEM and EDS that we can show visiting
} clients and use during technical demonstrations and presentations.
}
} Does anyone have, know, or can sell a video along these lines. The audience
} will be for the most part college educated but in non-technical subjects.
} Videos from manufacturers are acceptable.
}
} Thanks and please answer to email address: "robertco2-at-aol.com"
}
} Robert Sherman
}




From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Wed, 17 Jul 1996 18:50:49 -0400 (EDT)
Subject: Re: Need a SEM/EDS Video (fwd)

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The Education Committee of MSA has a large collection of videos on
microscopy. Among the videos are two fine videos on EDS by Dale Newberry.
Information on the videos is part of the MSA website at
www.msa.microscopy.com.

Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************

On Wed, 17 Jul 1996 RobertCO2-at-aol.com wrote:

} I consult to a place that does SEM for forensis and failure analyses. We are
} looking for an educational video on SEM and EDS that we can show visiting
} clients and use during technical demonstrations and presentations.
}
} Does anyone have, know, or can sell a video along these lines. The audience
} will be for the most part college educated but in non-technical subjects.
} Videos from manufacturers are acceptable.
}
} Thanks and please answer to email address: "robertco2-at-aol.com"
}
} Robert Sherman
}





From: JANET SUE FOLMER :      jfol-at-welchlink.welch.jhu.edu
Date: Wed, 17 Jul 1996 14:41:40 -0400 (EDT)
Subject: unsubscribe thankyou

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From: drouillon-solvay-at-e-mail.com
Date: Thu, 18 Jul 1996 05:17:15 EDT
Subject: LaB6 filament on a SEM (message corrected)

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Hello,


We ae contemplating the replacement of the tungsten filament on our SEM
(Cambridge Stereoscan 360) by a LaB6 source.
I met several users at the Summer School of SEM and Micronalysis at
Louvain-la-Neuve (Belgium) who expressed different views.
The "pro-LaB6" are delighted to use it.
The "anti-LaB6" people emphasize on the poor stability of the electron beam and
the mechanical brittleness of the source. Some have returned to the "old"
tungsten filament.
So, ex- or new LaB6 user, what is your opinion ?


Best regards,


Philippe Drouillon
Solvay Research and Technology
Brussels (Belgium)

Extra X400 information begins:
Originator
Name: Philippe (PDU) DROUILLON
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Subject: LaB6 filament on a SEM (message corrected)
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From: drouillon-solvay-at-e-mail.com
Date: Thu, 18 Jul 1996 05:16:54 EDT
Subject: LaB6 filament on a SEM

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Hello,


We ae contemplating the replacement of the tungsten filament on our SEM
(Cambridge Stereoscan 360) by a LaB6 source.
I met several users at the Summer School of SEM and micronalysis at
Louvain-la-Neuve (Belgium) who expressed different views.
The "pro-LaB6" are delighted to use it.
The "anti-LaB6" people emphasize on the poor stability of the electron beam and
the mehanical brittleness of the source. Some have returned to the "old"
tungsten filament.
So, ex- or new LaB6 user, what is your opinion ?


Best regards,


Philippe Drouillon
Solvay Research and Technology
Brussels (Belgium)

Extra X400 information begins:
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From: as-at-mcs.net (Alan Stone)
Date: Thu, 18 Jul 1996 07:15:11 -0500 (CDT)
Subject: LM-Looking for B&L reticle

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I am looking to purchase a Bausch & Lomb eyepiece reticle which is no longer
in production. It is described as a micrometer disc, PN 31-16-08. It has
100 lines divided into half units with longer lines at every 10 units. It
is used to measure 0.001" at 100X.

If you are a source or know of a source, I would appreciate some help.
Thanks in advance.

Alan Stone
Alan Stone





From: csbeneas :      csbeneas-at-wiccmail.weizmann.ac.il
Date: 18 Jul 1996 15:58:34 +0200
Subject: mat sci knifes for hard tissue

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Message-ID: {n1374416961.90105-at-wiccmail.weizmann.ac.il}

Do somebody have an expirience in a cutting the hard tissues by material
science diamond knifes? Do they give an appropriate quality of the sections
and how long they live? Thank you in advance, Elia Beniash





From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Thu, 18 Jul 1996 9:55:47 -0400
Subject: Re: LM-Looking for B&L reticle - reply

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Message-ID: {CE15EE3101F70300-at-mhs.unc.edu}
In-Reply-To: {CB15EE3101F70300-at-mhs.unc.edu}

B&L microscopy was purcased by Cambridg Instruments which in turn was
bought/merged with Leitz which chaned its name to Leica (they've had a
major indentity thing over there since the American Optical, AO, Reichert
Jung days). Any way their tel # is 800-248-0123.
best of luck

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Thu, 18 Jul 1996 8:12:55 -0400
Subject: Re: fluorescent bone specimens

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Message-ID: {C315EE3101F70300-at-mhs.unc.edu}
In-Reply-To: {AE15EE3101F70300-at-mhs.unc.edu}

Thomas.

you asked
} I have a couple of clients who want to coverslip some ground bone
specimens. The bones were labeled in vivo with fluorescent compounds
that
incorporate into the matrix. During the specimen prep, the alcohol fixed
bones are washed in xylene or acetone. Is there any reason not to use
Permount to coverslip? {

A good reason not to use Permount is that in contains flourescent
materials. Use one of the mounting medias that were developed for
flourescent techniques. I cant help you with the request on bone
staine but Cathy Sanderson (e-mail Histology-at-aol.com) has been working
with a multitude of bone techniques for many years and you might want to
e-mail her.
regards,

Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: Bernd Feja :      feja-at-ubaclu.unibas.ch
Date: Thu, 18 Jul 1996 16:07:04 +0200
Subject: polystyrene beads compositon

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Hi,
i'm using latex beads (polystyrene) as test objects for scattering
experiments in EM. For comparing Monte Carlo calculations i need the
exact composition data (in at%). My current data are 50% H, 50% C,
density 1.049 g/ccm. Does anyone have different or more precise values?

Thanks,
Bernd




From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Thu, 18 Jul 1996 10:17:30 -0800
Subject: Riber ion pump

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Message-Id: {v02110101ae142f996a39-at-[130.191.238.149]}
Mime-Version: 1.0
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greetings all

does anyone know if there is a US representative and contact number for the
manufacturer of Riber ion pumps?

thanks in advance

steve

Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Dr.
San Diego, CA 92182-4614
phone: (619)594-4523
fax:(619)594-5676
email:sbarlow-at-sunstroke.sdsu.edu






From: Paul Millard :      Paul_Millard-at-probes.com
Date: Thu, 18 Jul 1996 08:35:37 -0008
Subject: unsubscribe

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**********************************************************************
*** Paul J. Millard, Ph.D. Voice: (541) 465-4579 ***
*** Molecular Probes, Inc. FAX: (541) 344-6504 ***
*** 4849 Pitchford Avenue paulm-at-probes.com ***
*** Eugene, OR 97402-9144 ***
**********************************************************************




From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 18 Jul 96 12:30:12 EDT
Subject: Re: mat sci knifes for hard tissue

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Dear Dr. Beneash:

You may want to contact Dr. Charles Garber from SPI/Structure Probe. I have
seen him comment many times on the listserver about materials science diamond
knives and I believe he had also provided the type of information you are
looking for. Unfortunately, I did not save his comments. You can reach him at:

spi2spi-at-2spi.com or you can get some good information from the SPI web site at
http://www.2spi.com.

Good luck!

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
http://www.southbaytech.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Thu, 18 Jul 1996 11:28:12 -0700 (PDT)
Subject: Re: LM-Looking for B&L reticle

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Alan,
Call Klarmann Rulings, Inc., (800) 252-2401. They make all kinds of
eyepiece reticles. Measure the inside diameter of your eyepiece.
This is who our Leica dealer usually orders eyepiece reticles from. These
usually cost in the $50 range. We've purchased several different styles
for Zeiss, Wild, Nikon and Leitz eyepieces.


Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu



On Thu, 18 Jul 1996, Alan Stone wrote:

} I am looking to purchase a Bausch & Lomb eyepiece reticle which is no longer
} in production. It is described as a micrometer disc, PN 31-16-08. It has
} 100 lines divided into half units with longer lines at every 10 units. It
} is used to measure 0.001" at 100X.
}
} If you are a source or know of a source, I would appreciate some help.
} Thanks in advance.
}
} Alan Stone
} Alan Stone
}
}





From: Dave King (607)757-1248 T37/257-3 Ext DEKING-at-VNET.IBM.COM
Date: 18 Jul 1996 14:41:08 EDT
Subject: LaB6 filament on a Cambridge

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Message-Id: {199607181836.NAA05406-at-Sparc5.Microscopy.Com}

To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}
*** Reply to note of 07/18/96 06:37

We have a Cambridge S-250, and have run LaB6 for about 14 years.
We used to have some trouble with the "carbon leg" designed
mount. I burned a couple out in a few hundred hours (tip still
looked like new). Since going to Denka M3's, we've gotten 1 to 3K
hours per x-stal. I just took out or 1st M7, and it was OK, but
the x-stal bent over (way odd!), but that was after about 2500
hours. Our gun vacuum is an indicated 7x10-7 torr.

We like the high brightness and long life. We heat them up
slowly, and recently began using the 1st saturation, not the 2nd
saturation point, like we always have. Reportedly, that'll make
for even longer life. The current stability looks good, so we'll
stick with it.

W is a mess to clean up after, the life is short (~40 hours?),
and the brightness is low. For about $700/LaB6 tip, there's no
contest.

That's my 2 cents!

. {
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Thu, 18 Jul 1996 14:17:07 -0700
Subject: TEM available: Philips 201

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Message-Id: {v02140b04ae14563d26b2-at-[129.82.202.24]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I just gat a call from someone in Illinois who has a Philips EM201 for
sale. He needs to move it as soon as possible and will entertain all
offers.

For more information contact Michael Scami at 815-741-2222.

John
chandler-at-lamar.ColoState.EDU






From: Christine Powers :      cp-at-insitu.ummed.edu
Date: Thu, 18 Jul 1996 15:27:58 -0400 (EDT)
Subject: autofluorescence/tissue culture

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Does anyone know of a reference that discusses fixation induced
autofluorescence in cultured cells?
Thanks in advance.


Chris Powers for Joan Politz at UMass Med Center





From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Thu, 18 Jul 1996 11:17:48 -0500
Subject: Re: mat sci knifes for hard tissue

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In message {n1374416961.90105-at-wiccmail.weizmann.ac.il} "csbeneas" writes:
} Do somebody have an expirience in a cutting the hard tissues by material
} science diamond knifes? Do they give an appropriate quality of the sections
} and how long they live? Thank you in advance, Elia Beniash

I have used a materials science diamond knife, with a 55 degree cutting edge
angle, to section epoxy resin embedded eggshell from chicken, turtle and
platypus. I vacuum infiltrated the resin into the shell fragments and was able
to get reasonably good ultrathin sections of about 80 nm. The chicken shell was
the most difficult to section.

The larger knife edge angles, about 55 degrees, are important to give the edge
more durability to increase their useful life, they are less likely to chip,
even if it is diamond. Its difficult to give you a typical lifetime for these
knives, as it all depends on how much it is used, with what care and how it is
maintained. Usually, one does not pass a diamond knife around, just reserve it
for yourself or a specific highly skilled individual who knows how to use it
properly.

I've not sectioned bone, but would be interested to hear from anyone who has,
whether for LM or EM, and what staning methods were used.



Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996





From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Thu, 18 Jul 1996 11:33:00 -0500 (CDT)
Subject: Post Doc Position at Cambridge- TEM

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Colleague

Please send all replies directly to

wos1-at-cus.cam.ac.uk

and not to me.

Nestor
Your Friendly Neighborhood SysOp.


=============================================================================





From: W Owen Saxton :      wos1-at-cus.cam.ac.uk
Date: Thu, 18 Jul 1996 16:55:40 +0100 (BST)
Subject: for mailing list..

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Could the following be put on your mailing list / bulletin board?
Thanks! - Owen


TEM Automation: Post-doctoral position
--------------------------------------

We have a two-year post-doctoral position available for a project
involving the automation of TEM.

The EPSRC-funded project "Comprehensive automation of TEM" involves
developing a computer-based image acquisition and control system for
TEM, addressing not only the very high resolution mode but also other
modes together with start-up and shut-down procedures, condenser
stigmating, tilt-shift purity adjustment etc. as well as remote
computing. We envisage 1024sq CCD as well as conventional TV image
pickup, using 200kV and 400kV JEOL microscopes at the Departments of
Materials Science & Metallurgy and of Chemistry, in a PC/Windows
environment, probably using C++, perhaps Synoptics new IO package, and
perhaps a high speed array processor.

The ideal person would have experience both of TEM and of programming;
the latter is the more important requirement however. The post is
available from 1st October 1996, but the start date could be deferred
some months if necessary; salary on UK academic scales according to
age and experience, up to point 7 (15,154 pounds pa currently).

Please contact any of the project investigators for more information:
Dr W Owen Saxton Dept Materials Science & Metallurgy, Pembroke St, CB2 3QZ
email wos1-at-cam.ac.uk
Dr David M Holburn Dept Engineering, Trumpington St, Cambridge
email dmh-at-eng.cam.ac.uk
Dr Angus I Kirkland Dept Chemistry, Lensfield Rd, Cambridge, and
JEOL UK Ltd, Silvercourt, Watchmead, Welwyn Garden City, AL7 1LT
email aik10-at-cam.ac.uk

Those interested in the position should send a CV, list of publications,
and covering letter to Owen Saxton by 31st August; electronic and paper
submissions are both welcome.






From: Walt Bobrowski (313) 996-7814 :      bobroww-at-aa.wl.com
Date: Thu, 18 Jul 1996 14:54:00 -0400 (EDT)
Subject: Position Opening-In Situ Hybridization

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Mr-Received: by mta SRVR05.MUAS; Relayed; Thu, 18 Jul 1996 14:54:00 -0400
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Disclose-Recipients: prohibited

Senior Assistant/Associate Scientist

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At Parke-Davis our dedicated team members have made us a world leader in
developing innovative pharmaceutical products. As our strategy for
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Human Resources, Box CJM96080
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FAX: (313) 996-7617
E-mail: resume-at-aa.wl.com

Equal Opportunity Employer - Smoke Free Work Environment





From: m.dickson-at-unsw.edu.au (melvyn dickson)
Date: Fri, 19 Jul 1996 11:41:08
Subject: Re: LaB6 filament on a SEM

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To: microscopy-at-Sparc5.Microscopy.Com

In article drouillon-solvay-at-e-mail.com writes:
} From: drouillon-solvay-at-e-mail.com
} Date: Thu, 18 Jul 1996 05:16:54 EDT
} Subject: LaB6 filament on a SEM
} Hello,
} We are contemplating the replacement of the tungsten filament on our SEM
} (Cambridge Stereoscan 360) by a LaB6 source.
} I met several users at the Summer School of SEM and micronalysis at
} Louvain-la-Neuve (Belgium) who expressed different views.
} The "pro-LaB6" are delighted to use it.
} The "anti-LaB6" people emphasize on the poor stability of the electron beam and
} the mehanical brittleness of the source. Some have returned to the "old"
} tungsten filament.
} So, ex- or new LaB6 user, what is your opinion ?

We have been using LaB6 off and on in our S360 for 5-6 years. Enough to say
we have mixed feelings, not all positive by any means.

1. The ion pump system is fiddly to use, especially to get it started once
air has got into it. The column isolation valve over the objective lens is
prone to leaks on the O ring on the shaft and the shaft needs careful
polishing. The O ring on the valve itself has atmospheric pressure working
against it and needs to be precisely located to avoid leaks.

2. Careless users are especially prone to opening the isolation valve before
the chamber has pumped down. That puts the S360 out of action for the rest of
the day including the time it takes to repump and restart the ion pump.

3. The LaB6 emitter SEEMS to be rather mobile in the S360 gun. We had bad
experiences with Denka M3 emitters. The beam would drift out of line over
about 5 minutes. Entirely useless for EDS and pretty exasperating for
capturing an image. Cambridge suggest cleaning the Wehnelt aperture every
friday to reduce this drift but thats very fiddly indeed and actually doesn't
help that much. Oddly enough the Cambridge Electron Beam Litho writer in
Sydney uses Denka M3 emitters and they are superbly stable in that machine.

We have been using Kimball Lab6 mounts with better results but after a time
drift will still occur. Right now we have a tungsten emitter in but we still
use the Ion pump system so as to retain a consistent operating procedure, and
to try for better emitter life.

Very similar results were noted in the S-360 at the Australian National
University so it looks like a generic problem.

Kimball have done a lot of work with their LaB6 emitters in S-360 microscopes
so I reckon they have the best backup if you have problems.

In the end we bypassed the problem by going to FESEMs and we no longer try to
push the S-360 to the limit of performance.

Mel Dickson
UNSW, Sydney.




From: qian-at-elmo.tech.nwu.edu (Weida Qian)
Date: Thu, 18 Jul 1996 21:48:34 -0500
Subject: unsubscribe

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======================================================
Weida Qian
Department of Materials Science & Engineering
Northwestern University
Evanston, IL 60208

Ph: (847)-491-3594; Fax: (847)-491-7820
E-mail:qian-at-elmo.tech.nwu.edu
======================================================






From: A. Greene :      ablue-at-mail.io.com
Date: Thu, 18 Jul 1996 21:30:51 -0500 (CDT)
Subject: Re: Riber ion pump

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Hello Steve, There is a company in California which rebuilds Getter
Pumps. They do great work and it costs less than buying a new pump. If you
cannot wait for the rebuild, they have used pumps for sale. Here is the address:

Dunaway Stockroom Corp.
1305 Space Park Way
Mountain View California
94043

Phone 800/446-8811 FAX 415/965-0764

Personally, I think you would do well using a better quality pump than Riber.

Good Luck. Alex Greene
Scientific Instrumentation Services, Inc.
Austin, Texas


At 10:17 AM 7/18/96 -0800, you wrote:
} greetings all
}
} does anyone know if there is a US representative and contact number for the
} manufacturer of Riber ion pumps?
}
} thanks in advance
}
} steve
}
} Dr. Steven Barlow
} EM Facility/Biology Dept.
} San Diego State University
} 5500 Campanile Dr.
} San Diego, CA 92182-4614
} phone: (619)594-4523
} fax:(619)594-5676
} email:sbarlow-at-sunstroke.sdsu.edu
}
}
}
}





From: Probing & Structure :      pns-at-ultra.net.au
Date: Fri, 19 Jul 1996 14:59:54 +1000
Subject: polystyrene beads compositon

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On Thu, 18 Jul 1996 16:07:04 +0200, Bernd Feja {feja-at-ubaclu.unibas.ch} wrote:

Hi,
i'm using latex beads (polystyrene) as test objects for scattering
experiments in EM. For comparing Monte Carlo calculations i need the
exact composition data (in at%). My current data are 50% H, 50% C,
density 1.049 g/ccm. Does anyone have different or more precise values?

Thanks,
Bernd
***********************

Interesting, you may not have to contend with only H & C.
To avoid clumping of the particles the solution used is not superpure water.
A little conductivity is required and usually sulfate groups are used to act
on the surface of the latex particles.

Perhaps this will not affect your results, perhaps you can wash the
particles with superpure water and accept some clumping.
I suggest that you contact Duke Scientific Corp, in California; I only have
their fax number, which is: 1 415 424 1158. They have done a lot of work on
calibration processes using latex particles and should have all required
information.

Jim Darley
Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site http://www.ultra.net.au/~pns/
***********************





From: ebs-at-ebsciences.com
Date: Fri, 19 Jul 1996 07:32:55 -0500
Subject: Re: LaB6 filament on a Cambridge

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I want to add a few comments to the post from Dave King. Brightness of LaB6
tips is estimated at about 10 times that of tungsten. Normal lifetime
should exceed 500 hours (not everyone gets the lifetimes Dave reported in
his post, mainly because his vacuum is better than most).

We always recommend that LaB6 cathodes be operated at just "below" the
second saturation for best performance.

We have a binder full of technical information on the Denka LaB6 cathodes
which we sell which we would be happy to send to anyone (please e-mail me
with your complete s-mail address).

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: ebs-at-ebsciences.com
Date: Fri, 19 Jul 1996 08:09:00 -0500
Subject: Re: LaB6 filament on a SEM

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Melvyn Dickson reported some problems with the use of LaB6 cathodes which I
would like to address.

LaB6 cathode designs using tungsten wire for mounting the LaB6 crystal, like
the Denka Model 3, will "drift" as the tungsten reacts to heat. For EDS,
critical dimension analysis or line width measurement (or any other
application where the beam needs to stay in one place for an extended period
of time), I would recommend using an LaB6 cathode where the crystal is
mounted on fixed molybdenum posts, such as the Denka Model 7. This design
offers superior mechanical stability.

For most other SEM applications, the Model 3 should work well, and is, in
fact, what LEO presently supplies installed in their new LaB6 instruments.

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: vtanner-at-codon.nih.gov (Virginia A. Tanner)
Date: Fri, 19 Jul 1996 10:28:57 -0500
Subject: Re: LaB6 filament on a SEM

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subscribe microscopy vtanner-at-codon.nih.gov.

*******************************************************************
Virginia A. Tanner Crocker
NIH
NINDS EM Facility,
Bldg 36, Room 3B24
Bethesda, MD 20892

phone: 301-496-0579 V/TT
Fax: 301-402-6875
e-mail: vtanner-at-codon.nih.gov
*******************************************************************






From: kna101-at-utdallas.edu
Date: Fri, 19 Jul 1996 08:01:38 -0500 (CDT)
Subject: Re: mat sci knifes for hard tissue

Contents Retrieved from Microscopy Listserver Archives
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Hi Gib,

On your question reguarding LM or EM sectioning and staining of bone,
I've done several procedures for LM with decalcified temporal bones of
humans and rodents. I've embedded in medcast, and JB-4 and sectioned
with a glass knife and I've embedded in parafin and parlodion (celloidin)
and sectioned on a steel knife. For staining, toluidine blue gives a
nice, metachromatic stain in JB-4, but not in medcast and
hematoxylin/eosin gives a nice metachromatic stain in both parafin and
celloidin [the hematoxylin formula that works the best is for Harris'
hematoxolyn]. The medcast tissue was for EM study, but we were mainly
interested in the inner ear tissue embedded in the bone, so I can't
comment on how well the bone stained. We had no problem sectioning it
on standard diamond knives, as it was decalcified. I've tried embedding
the bones in medcast without decalcification (no vacuum) and sectioning
on glass knives, but only the outermost millimeter of bone was
infiltrated with resin, so the center portion just crumbled. Usually, we
are interested in the tissue embedded in the bone and not the bone
itself, so decalcification is the easiest way to go.

Karen Robinson Pawlowski




From: s002swh-at-desire.wright.edu (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Fri, 19 Jul 1996 19:12:30 -0500
Subject: RE: LM Tissue Embedding

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APB,
I'm trying to fix, dehydrate and embed in paraffin, cells in a
suspension of collagen and agarose. The agarose is giving me fits. I'm
trying different concentrations of formalin, and have tried xylene and
ethlyne glycol as transitions to wax. Two problems are occurring. 1) the
center of the tissue is not embedding. 2) When sectioning the agarose
shears and comes out of the paraffin.
Any suggestions would be helpful,
TIA

Steve Hendrix
Wright State University
s002swh-at-desire.wright.edu








From: MCCLELLAN DAVID S :      MCCLELLAN_DAVID_S-at-lilly.com (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Fri, 19 Jul 1996 19:09:46 -0500
Subject: Reflected light, DIC, and Kohler Illumination

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Light microscopists, I need your help!
Our lab has a Zeiss Axioplan microscope with DIC capability. The DIC has been
used with transmitted light, but are the optics such that this will work with
transmitted light as well? Also, can you Kohler illuminate in the reflected
light mode?

Let me say in advance, that I appreciate your help in providing information
regarding these questions. Thanks!

David McClellan
Eli Lilly & Co.
e-mail: DM-at-lilly.com








From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Sat, 20 Jul 1996 10:12:39 -0500
Subject: Administrivia.. How to Subscribe/Unsubscribe/Change Address

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NIH Library Circulation {circ-at-NIHRRLIB.NCRR.NIH.GOV} ,
Interlibrary Loan Books {ILL-at-NIHRRLIB.NCRR.NIH.GOV} ,
"Stroman, Rosalie" {STROMANR-at-NIHRRLIB.NCRR.NIH.GOV} ,
"Sunderland, Ed" {SUNDERLE-at-NIHRRLIB.NCRR.NIH.GOV} ,
"Adams, Anthony J." {ADAMSA-at-dirpc.nimh.nih.gov} ,
ADAMSJ-at-dirpc.nimh.nih.gov, ADAMSN-at-dirpc.nimh.nih.gov
To: AGUNYEGB-at-dirpc.nimh.nih.gov, AKILM-at-dirpc.nimh.nih.gov,
ALAMINH-at-dirpc.nimh.nih.gov,
"Allen, Robert C." {ALLENR-at-dirpc.nimh.nih.gov} ,
"Apud, Jose A." {APUDJ-at-dirpc.nimh.nih.gov} , ATTOHN-at-dirpc.nimh.nih.gov,
"Au, Allen" {AUA-at-dirpc.nimh.nih.gov} , BACAS-at-dirpc.nimh.nih.gov,
BACHUSS-at-dirpc.nimh.nih.gov, BACICM-at-dirpc.nimh.nih.gov,
BADGERH-at-dirpc.nimh.nih.gov
To: BAKERI-at-dirpc.nimh.nih.gov, BALIG-at-dirpc.nimh.nih.gov,
BASSIWAC-at-dirpc.nimh.nih.gov, BATTSD-at-dirpc.nimh.nih.gov,
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BERTOLIA-at-dirpc.nimh.nih.gov, BESTA-at-dirpc.nimh.nih.gov,
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"Bigelow, Llewellyn B." {BIGELOWL-at-dirpc.nimh.nih.gov} ,
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To: BOWLESS-at-dirpc.nimh.nih.gov, BRADYC-at-dirpc.nimh.nih.gov,
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"Burwell, Veronica A." {BURWELLV-at-dirpc.nimh.nih.gov} ,
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To: DIGLISIS-at-dirpc.nimh.nih.gov, DILLONCO-at-dirpc.nimh.nih.gov,
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To: GILROYR-at-dirpc.nimh.nih.gov, GLENNF-at-dirpc.nimh.nih.gov,
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"Goldstein, Mark" {GOLDSTEM-at-dirpc.nimh.nih.gov} ,
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To: HEINZA-at-dirpc.nimh.nih.gov, "Henter, Ioline" {HENTERI-at-dirpc.nimh.nih.gov} ,
HIRSHMAG-at-dirpc.nimh.nih.gov, HITRIA-at-dirpc.nimh.nih.gov,
"Hodges, Gerald L." {HODGESG-at-dirpc.nimh.nih.gov} ,
HOWARDA-at-dirpc.nimh.nih.gov, HYDET-at-dirpc.nimh.nih.gov,
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JAMESD-at-dirpc.nimh.nih.gov,
"James, Johnnie M." {JAMESJ-at-dirpc.nimh.nih.gov}
To: JOHNSONS-at-dirpc.nimh.nih.gov, JONESD-at-dirpc.nimh.nih.gov,
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LILLRANS-at-dirpc.nimh.nih.gov, LIPSKAB-at-dirpc.nimh.nih.gov,
LIPSKIW-at-dirpc.nimh.nih.gov
To: LONGR-at-dirpc.nimh.nih.gov, "Lyons, William E." {LYONSW-at-dirpc.nimh.nih.gov} ,
MASSERAJ-at-dirpc.nimh.nih.gov, MCKIEH-at-dirpc.nimh.nih.gov,
"Meltzer, Marc" {MELTZERM-at-dirpc.nimh.nih.gov} ,
"Merril, Carl R." {MERRILC-at-dirpc.nimh.nih.gov} ,
MILLERG-at-dirpc.nimh.nih.gov, MILLERJ-at-dirpc.nimh.nih.gov,
MILLERM-at-dirpc.nimh.nih.gov, MOTTS-at-dirpc.nimh.nih.gov,
MURRAYA-at-dirpc.nimh.nih.gov
To: MYSLOBOM-at-dirpc.nimh.nih.gov, NEVERSA-at-dirpc.nimh.nih.gov,
"Nguyen, Khoi" {NGUYENK-at-dirpc.nimh.nih.gov} ,
OLEARYK-at-dirpc.nimh.nih.gov, PALTANJ-at-dirpc.nimh.nih.gov,
PHILLIPI-at-dirpc.nimh.nih.gov, PLATTH-at-dirpc.nimh.nih.gov,
PODELLD-at-dirpc.nimh.nih.gov, PURCELLC-at-dirpc.nimh.nih.gov,
QUARTEYR-at-dirpc.nimh.nih.gov, RAEDLERT-at-dirpc.nimh.nih.gov,
REDDICKL-at-dirpc.nimh.nih.gov
To: REIDJ-at-dirpc.nimh.nih.gov, RIDLEYW-at-dirpc.nimh.nih.gov,
RODEFFEC-at-dirpc.nimh.nih.gov, ROGERSE-at-dirpc.nimh.nih.gov,
ROSIERF-at-dirpc.nimh.nih.gov, RUCKERL-at-dirpc.nimh.nih.gov,
RUMBAUGJ-at-dirpc.nimh.nih.gov, SCHILDTS-at-dirpc.nimh.nih.gov,
"Seals, Troy E." {SEALST-at-dirpc.nimh.nih.gov} ,
SEGALR-at-dirpc.nimh.nih.gov,
"MS:NIHNIMH/DIRPC/SHANNOWC" {SHANNOWC-at-dirpc.nimh.nih.gov}
To: SLADEK-at-dirpc.nimh.nih.gov, SLYDELLP-at-dirpc.nimh.nih.gov,
SMITHE-at-dirpc.nimh.nih.gov, SNITKOVY-at-dirpc.nimh.nih.gov,
SPOORE-at-dirpc.nimh.nih.gov, SRIPATHC-at-dirpc.nimh.nih.gov,
"Staub, Richard A." {STAUBR-at-dirpc.nimh.nih.gov} ,
STEARNSA-at-dirpc.nimh.nih.gov, STICKELD-at-dirpc.nimh.nih.gov,
STOKESA-at-dirpc.nimh.nih.gov,
"Strotkamp, Mollie P." {STROTKAM-at-dirpc.nimh.nih.gov}
To: STURTEVJ-at-dirpc.nimh.nih.gov, SUNDBERL-at-dirpc.nimh.nih.gov,
"Sydnor, Barry C." {SYDNORB-at-dirpc.nimh.nih.gov} ,
"Tolbert, Theresa L." {TOLBERTT-at-dirpc.nimh.nih.gov} ,
"Tompkins, Dera" {TOMPKIND-at-dirpc.nimh.nih.gov} ,
TRAKICA-at-dirpc.nimh.nih.gov,
"Truckenmiller, Emmy" {TRUCKENE-at-dirpc.nimh.nih.gov} ,
TURNERA-at-dirpc.nimh.nih.gov, TURNERM-at-dirpc.nimh.nih.gov,
UNIT2-at-dirpc.nimh.nih.gov
To: UNIT3-at-dirpc.nimh.nih.gov, URBINAR-at-dirpc.nimh.nih.gov,
"VanderPuten, Dale" {VANDERPD-at-dirpc.nimh.nih.gov} ,
"Vawter, Marquis P." {VAWTERM-at-dirpc.nimh.nih.gov} ,
VELAF-at-dirpc.nimh.nih.gov, VENABLED-at-dirpc.nimh.nih.gov,
"Waldman, Ivan N." {WALDMANI-at-dirpc.nimh.nih.gov} ,
"Washart, Karen M." {WASHARTK-at-dirpc.nimh.nih.gov} ,
WATSKYE-at-dirpc.nimh.nih.gov, WATSONC-at-dirpc.nimh.nih.gov
To: WATSONT-at-dirpc.nimh.nih.gov, WEBSTERM-at-dirpc.nimh.nih.gov,
WEICKERT-at-dirpc.nimh.nih.gov, WEINBERD-at-dirpc.nimh.nih.gov,
WEIRICHM-at-dirpc.nimh.nih.gov, WESCHLEM-at-dirpc.nimh.nih.gov,
WILDERK-at-dirpc.nimh.nih.gov,
"Williams, Jamilah L." {WILLIAJL-at-dirpc.nimh.nih.gov} ,
WILLIAJR-at-dirpc.nimh.nih.gov, WILLIAMJ-at-dirpc.nimh.nih.gov,
WOLFS-at-dirpc.nimh.nih.gov, WOOLFOLJ-at-dirpc.nimh.nih.gov
To: WRIGHTR-at-dirpc.nimh.nih.gov, WYATTR-at-dirpc.nimh.nih.gov,
WYNNC-at-dirpc.nimh.nih.gov, YANGF-at-dirpc.nimh.nih.gov,
"Yang, Hsiu-Ying T." {YANGH-at-dirpc.nimh.nih.gov} ,
YATESV-at-dirpc.nimh.nih.gov, "Zullo, Steve" {ZULLOS-at-dirpc.nimh.nih.gov} ,
"Conn, Kathleen" {connk-at-irp.nimh.nih.gov} ,
"Kelly, Dianne" {KellyD-at-odepsm2.od.nih.gov} ,
karau {kuherka-at-badlands.nodak.edu}
To: iomegafeedback {routh-at-iomega.com} , boblinda {103654,1625-at-CompuServe.COM} ,
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To: arkerlav {arkerlav-at-tigr.org} , avf {avf-at-helix.nih.gov} ,
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To: "Zoltick, Brad J {bz1h} " {bz1h-at-nih.gov} , CHANGE {CHANGE-at-nih.gov} ,
cheri {cheri-at-bgsun2.nimh.nih.gov} ,
Very Merry Cheri Berry {cheris-at-phoenix.princeton.edu} ,
Mike Chipperfield {chipper-at-gdb.org} , circ {circ-at-nih.gov} ,
circadm {circadm-at-nih-library.ncrr.nih.gov} , coolman {Coolman-at-cnn} ,
"CSAR-L%NIHLIST.BITNET" {CSAR-L%NIHLIST.BITNET-at-CU.NIH.GOV}
To: alan {cuaez-at-ecom2.ecn.bgu.edu} , cuaez {cuaez-at-ecom5.ecn.bgu.edu} ,
"Whitley, Ed {cw25f} " {cw25f-at-nih.gov} , dab {dab-at-ray.nlm.nih.gov} ,
sergodani {dani-at-protein.osaka-u.ac.jp} , davidkgdb {davidk-at-gdb.org} ,
"Cohen, Deborah {dc26a} " {dc26a-at-nih.gov} , dec {dec-at-helix.nih.gov} ,
Dale Graham {degraham-at-helix.nih.gov} ,
"Graham, Dale {dg6n} " {dg6n-at-nih.gov}
To: image-dove {dove-at-uthscsa.edu} , ed {edw-at-nchgr.nih.gov} ,
Leslie Emmert {emmertbk-at-helix.nih.gov} ,
npr-evaluate {evaluation-at-npr.ai.mit.edu} ,
Evgeni Selkov {evgeni-at-mcs.anl.gov} , ffwd2 {ffwd-at-washpost.com} ,
fgsc {fgsc-at-KUHUB.CC.UKANS.EDU} , "5'3'" {fivprime-at-ix.netcom.com} ,
flybrain {flybrain-request-at-gla.ac.uk} ,
"Fr. Jack Frerker" {FR_JACK-at-crc.stmartin.edu} , GXY {GXY-at-CU.NIH.GOV}
To: hackett {hackett-at-focus.nlm.nih.gov} , harbourt {harbourt-at-nlm.nih.gov} ,
hhs {hhsnews-at-dhhs.ssw.dhhs.gov} , HOPOS-L {HOPOS-L-at-UKCC.UKY.EDU} ,
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Ian Clements {IANC-at-probes.mhs.compuserve.com} ,
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To: Yalebetsy {jasiorkobl-at-maspo2.mas.yale.edu} ,
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To: kt35o-at-nih.gov, karyn {ku-at-helix.nih.gov} ,
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kurs {kurs-at-novo.dk} , "Weeks, Kenneth A {kw1w} " {kw1w-at-nih.gov} ,
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To: grossmanl {lg-at-cmb.biosci.wayne.edu} , listproc {listproc-at-soils.umn.edu} ,
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To: MayranPE {MayranPE_at_ABD-US-PO3-at-ccip.perkin-elmer.com} ,
Midland primers {mcrc-at-WLN.COM} , science {membership-at-aaas.org} ,
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Microscopy-request {Microscopy-request-at-Sparc5.Microscopy.Com}
To: microscopy {Microscopy-at-Sparc5.Microscopy.Com} ,
"Macville, Merryn {mm292q} " {mm292q-at-nih.gov} ,
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To: embl {netserv%embl.BITNET-at-CU.NIH.GOV} ,
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notw {notw-request-at-nine.org} ,
NPR-Mail-Server {NPR-Mail-Server-at-LINCOLN.AI.MIT.EDU} ,
NW Dharma {nwdharma-at-accessone.com} ,
open-meeting {Open-Meeting-at-LINCOLN.AI.MIT.EDU} ,
Laszlo Orban {orban-at-hubi.abc.hu}
To: probes-cs {order-at-probes.mhs.compuserve.com} ,
midlprimers {orders-at-oligos.com} ,
conscious-owner {owner-jcs-online-at-psyche.zynet.co.uk} ,
"'participate'" {participate-at-LINCOLN.AI.MIT.EDU} ,
opmtgpart {participation-at-LINCOLN.AI.MIT.EDU} ,
rogan {pete-at-disomy.peds.hmc.psu.edu} ,
"Lemkin, Peter F {pl1a} " {pl1a-at-nih.gov} , rd {rd-at-zeus.ucsd.edu} ,
doolittle {rdoolittle-at-ucsd.edu}
To: retrieve-help {retrieve-help-at-ncbi.nlm.nih.gov} ,
retrieve {retrieve-at-ncbi.nlm.nih.gov} ,
sallie {RichardS-at-BDG10.NIDDK.NIH.GOV} ,
"Michael H. Rivner, M.D." {rivner%emg2-at-emgmhs.mcg.edu} ,
roganp {rogan-at-ncifcrf.gov} ,
Robert Pearlstein {rpearls-at-hawk.dcrt.nih.gov} ,
"bob's molec models" {rpearls-at-helix.nih.gov} ,
rpearlstein {rpearls-at-helix.nih.gov} , rs137o-at-nih.gov, rs200d-at-nih.gov
To: markey {s_markey-at-codon.nih.gov} , saccone {saccone-at-area.ba.cnr.it} ,
easchon {schon-at-cuccfa.ccc.columbia.edu} , sclark {sclark-at-pop.nih.gov} ,
sibbald {sibbald-at-embl-heidelberg.de} ,
sibbald {sibbald-at-qucis.queensu.ca} , istrailprt {sistrai-at-sandia.gov} ,
"'sharon chamberlain'" {slc2-at-aretha.jax.org} ,
"Markey, Sanford P {sm13q} " {sm13q-at-nih.gov}
To: Gary Smejkal {SMEJKAG-at-cesmtp.ccf.org} ,
"SMTP%\"circ-at-nihrrlib.ncrr.nih.gov\"%master.dnet" {SMTP%"circ-at-nihrrlib.ncrr.nih.gov"%master.dnet-at-dxi.nih.gov} ,
"SMTP%\"cuaez-at-ecom2.ecn.bgu.edu\"%master.dnet" {SMTP%"cuaez-at-ecom2.ecn.bgu.edu"%master.dnet-at-dxi.nih.gov} ,
"SMTP%\"degraham-at-helix.nih.gov\"%master.dnet" {SMTP%"degraham-at-helix.nih.gov"%master.dnet-at-dxi.nih.gov}
To: "SMTP%\"SemblyE-at-neon.bprc.nih.gov\"%master.dnet" {SMTP%"SemblyE-at-neon.bprc.nih.gov"%master.dnet-at-dxi.nih.gov} ,
jsnider {sniderjv-at-perkin-elmer.com} ,
SniderJV {SniderJV_at_ABD-US-FLD-at-ccip.perkin-elmer.com} ,
SniderJV {SniderJV_at_ABD-US-PO3-at-ccip.perkin-elmer.com} ,
microworld email {spb-at-wwa.com} , microworld {spignole-at-ix.netcom.com} ,
sheilap {spressman-at-mailgate.csmc.edu}
To: molecmodel {staff-at-hawk.dcrt.nih.gov} ,
"Stephen D. Fuller" {Stephen.Fuller-at-embl-heidelberg.de} ,
goldberg {stgoldberg-at-aol.com} , stromanr {stromanr-at-nih.gov} ,
strzel {strzel-at-speck.niddk.nih.gov} ,
strzel {strzel-at-spider.niddk.nih.gov} ,
"'dani'" {sudani-at-unix.horizontes.com.br} ,
nprsurveys {surveys-at-LINCOLN.AI.MIT.EDU} ,
npreview {surveys-at-town-hall.ai.mit.edu}
To: tanya {tanya-at-imeg.bio.psu.edu} , tberg {tberg-at-u.washington.edu} ,
clontech {tech-at-clontech.com} ,
probes-ts {tech-at-probes.mhs.compuserve.com} ,
Barb Trask {trask-at-biotech.washington.edu} , tried {tried-at-nchgr.nih.gov} ,
"Sargent, Tom (ts1r)" {ts1r-at-nih.gov} ,
twoolf {twoolf-at-ix10.ix.netcom.com} , twoolf {twoolf-at-ix6.ix.netcom.com} ,
twoolf {twoolf-at-ix7.ix.netcom.com}
To: update {update-at-ncbi.nlm.nih.gov} , vaytek {vaytek-at-ins.infonet.net} ,
verify {verify-at-cnet.com} , walker {walker-at-nlm.nih.gov} ,
wayne {wayne-at-helix.nih.gov} , wehart {wehart-at-cs.sandia.gov} ,
wehart {wehart-at-kiva.cs.sandia.gov} , hartprt {wehart-at-sandia.gov} ,
wilkes {wilkes-at-resgen.com} , wrengels {wrengels-at-facstaff.wisc.edu} ,
engels {wrengels-at-MACC.WISC.EDU}
To: bobzrg {zahr50-at-resgen.com} , zullosun {zullo-at-bgsun2.nimh.nih.gov} ,
zullo {zullo-at-helix.nih.gov}

G'day Colleague...

Just a posting to remind everyone of the correct procedure to
subscribe, unsubscribe and change addresses. You ALL will have
received these instructions when you initially subscribed, however,
I'll wager a beer or two that most of you have thrown out the
instructions, and I'll bet I come out ahead.

Remember the critical parameter in this system is YOUR EMAIL ADDRESS
you must unsubscribe the address which you originally sent in,
if you mail is being forwarded or you are using an alias, then you
must still provide the server with the ORIGINAL address. The server has
no idea that your mail if being forwarded by another computer.
and in this case your unsubscribe attempts will fail should you
provide your forwarding address instead of the original.



Cheers...
Nestor
Your Friendly Neighborhood SysOp
=========================================================================



How do I subscribe?
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I'm Going on Vacation/To A Meeting.... what should I do?
-------------------------------------------------

If your mail box is large enough then nothing.

If you don't want to collect your messages then simply unsubscribe
and send in a subscription request when you return

Never, Never, remain subscribed with your Email program/system to
respond
to all Email with an automatic message

"I'm out-of-town will respond to your message when
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This can create monsterous semi-infinite recursion loops
in the Email system. Some of you will recall we had a
massive one
a year or so ago and it took nearly a week to clear up,
with literally
hundreds of thousands of messages cycling through the Internet
as messages where bouncing all over the world. The current
software configuration minimizes the possiblity of this
happening
however, nothing is impossible and I want to make sure it
doesn't
reoccur.







From: John Best :      jbest-at-vicon.net
Date: Sat, 20 Jul 1996 16:03:00 -0700
Subject: Need EDAX 9900 keyboard

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-ID: {31F165A4.6E97-at-vicon.net}

Hello all,

Does anyone have a suggestion on how I might obtain a keyboard for an
EDAX PV9900 EDS? Also, a set of service schematics would be nice.

Thank You,
John Best -- ELMDAS Co.
jbest-at-vicon.net





From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: 20 Jul 96
Subject: polystyrene beads compositon

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199607202124.RAA27980-at-mime2.prodigy.com}
X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc04

-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #3.0 ] --

In response to the recent string:
================================================================
} i'm using latex beads (polystyrene) as test objects for scattering
experiments
} in EM. For comparing Monte Carlo calculations i need the exact
composition data
} (in at%). My current data are 50% H, 50% C, density 1.049 g/ccm. Does
anyone
} have different or more precise values? (Bernd)
==========================================================}
} Interesting, you may not have to contend with only H & C.
} To avoid clumping of the particles the solution used is not superpure
water. A
} little conductivity is required and usually sulfate groups are used to
act on
} the surface of the latex particles. (Jim Darley)
=========================================================
What is being referred to is the surfactant used during the
polymerization of the latex emulsion. In the past, the surfactant was
sodium lauryl sulfate, or (almost) plain ordinary "soap". Some emulsion
chemists even refered to it not as a surfactant, but as the "soap".
Today, there are more sophisticated surfactants used, but in any case,
on a mass basis, the amount present is very low, down to fractions of a
percent in composition. After all, it is was more than that, it would
not be a surfactant. I have never heard of anyone having problems with
their calculations with such small amounts of surfactant present in the
suspension.

Of course, there are all kinds of latex emulsions, ones that DO have
larger amounts of surfactant present, but the suspensions typically sold
by those offering EM supplies, such as SPI, select suspensions that are
very low in terms of surfactant concentration, in order to have "clean"
looking latex spheres when viewed by TEM or SEM.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.
com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.
com


Look for us!
############################
WWW: http://www.2spi.com
############################
=====================================================




From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Sun, 21 Jul 1996 12:55:39 -0500
Subject: Belgian Society meeting

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

Message-Id: {199607211751.MAA04272-at-Sparc5.Microscopy.Com}
X-Sender: zaluzec-at-microscopy.com
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

REGARDING Belgian Society meeting

Dear Colleagues,

On December 12 and 13, 1996, the Belgian and Dutch Societies for Microscopy
organise a joint meeting in Gent. You can find general information and the
program at our wedsite at

http://www.ruca.ua.ac.be/~BVM_SBM/progr_net.html

Just check it out !

Nick Schryvers








From: Ron Neumeyer :      micron-at-bc.sympatico.ca
Date: Sun, 21 Jul 1996 14:50:29 -0700 (PDT)
Subject: Need Zeiss/Leitz accessories

Contents Retrieved from Microscopy Listserver Archives
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I am looking for the following:

(1) Zeiss West dry darkfield condenser with holder Z (0.75/0.85)
(2) Zeiss West planopo 25x phase 3, or 25x planapo bright field objective
(160mm)
(3) Leitz Ortholux nosepiece

Regards,

Ron Neumeyer

phone 604-582-2552
fax 604-623-6239





From: Maite.Caldes-at-cnrs-imn.fr
Date: Mon, 22 Jul 1996 13:02:37 +0200
Subject: Need Zeiss/Leitz accessories

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


unsubcribe




From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Mon, 22 Jul 1996 10:27:02 +0000 (GMT)
Subject: Re: e-mail address change

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html

NIH Library Circulation {circ-at-NIHRRLIB.NCRR.NIH.GOV} ,
Interlibrary Loan Books {ILL-at-NIHRRLIB.NCRR.NIH.GOV} ,
"Stroman, Rosalie" {STROMANR-at-NIHRRLIB.NCRR.NIH.GOV} ,
"Sunderland, Ed" {SUNDERLE-at-NIHRRLIB.NCRR.NIH.GOV} ,
"Adams, Anthony J." {ADAMSA-at-dirpc.nimh.nih.gov} ,
ADAMSJ-at-dirpc.nimh.nih.gov, ADAMSN-at-dirpc.nimh.nih.gov,
AGUNYEGB-at-dirpc.nimh.nih.gov, AKILM-at-dirpc.nimh.nih.gov,
ALAMINH-at-dirpc.nimh.nih.gov,
"Allen, Robert C." {ALLENR-at-dirpc.nimh.nih.gov} ,
"Apud, Jose A." {APUDJ-at-dirpc.nimh.nih.gov} , ATTOHN-at-dirpc.nimh.nih.gov,
"Au, Allen" {AUA-at-dirpc.nimh.nih.gov} , BACAS-at-dirpc.nimh.nih.gov,
BACHUSS-at-dirpc.nimh.nih.gov, BACICM-at-dirpc.nimh.nih.gov,
BADGERH-at-dirpc.nimh.nih.gov, BAKERI-at-dirpc.nimh.nih.gov,
BALIG-at-dirpc.nimh.nih.gov, BASSIWAC-at-dirpc.nimh.nih.gov,
BATTSD-at-dirpc.nimh.nih.gov, BENIGNOG-at-dirpc.nimh.nih.gov,
BERMANK-at-dirpc.nimh.nih.gov, BERTOLIA-at-dirpc.nimh.nih.gov,
BESTA-at-dirpc.nimh.nih.gov, BIBERC-at-dirpc.nimh.nih.gov,
"Bigelow, Llewellyn B." {BIGELOWL-at-dirpc.nimh.nih.gov} ,
BISWASB-at-dirpc.nimh.nih.gov, BOWERC-at-dirpc.nimh.nih.gov,
BOWLESS-at-dirpc.nimh.nih.gov, BRADYC-at-dirpc.nimh.nih.gov,
BRANCHC-at-dirpc.nimh.nih.gov, BROUHAA-at-dirpc.nimh.nih.gov,
BROWNE-at-dirpc.nimh.nih.gov, BROWNR-at-dirpc.nimh.nih.gov,
BRYANTT-at-dirpc.nimh.nih.gov,
"Burwell, Veronica A." {BURWELLV-at-dirpc.nimh.nih.gov} ,
CALBERTM-at-dirpc.nimh.nih.gov, CALLICOJ-at-dirpc.nimh.nih.gov,
CAMPBELJ-at-dirpc.nimh.nih.gov, CARPENTC-at-dirpc.nimh.nih.gov,
CERVENAJ-at-dirpc.nimh.nih.gov, CHRISTIK-at-dirpc.nimh.nih.gov,
CLAUSNIL-at-dirpc.nimh.nih.gov, COGGIANM-at-dirpc.nimh.nih.gov,
COLLINSN-at-dirpc.nimh.nih.gov, CONEJERC-at-dirpc.nimh.nih.gov,
CONLEYC-at-dirpc.nimh.nih.gov, COPPOLAR-at-dirpc.nimh.nih.gov,
"Creed, G. Joseph" {CREEDG-at-dirpc.nimh.nih.gov} ,
DAMADZIR-at-dirpc.nimh.nih.gov, DEHAVENP-at-dirpc.nimh.nih.gov,
DICKINSD-at-dirpc.nimh.nih.gov, DIGLISIS-at-dirpc.nimh.nih.gov,
DILLONCO-at-dirpc.nimh.nih.gov, DUQUETTM-at-dirpc.nimh.nih.gov,
EDWARDSM-at-dirpc.nimh.nih.gov, EGANM-at-dirpc.nimh.nih.gov,
ELKASHEA-at-dirpc.nimh.nih.gov, FINNK-at-dirpc.nimh.nih.gov,
FOOKSM-at-dirpc.nimh.nih.gov, FREEDW-at-dirpc.nimh.nih.gov,
FREEMAND-at-dirpc.nimh.nih.gov, GAUSEJ-at-dirpc.nimh.nih.gov,
GEORGENP-at-dirpc.nimh.nih.gov, GILCHRIA-at-dirpc.nimh.nih.gov,
GILROYR-at-dirpc.nimh.nih.gov, GLENNF-at-dirpc.nimh.nih.gov,
GOLDBERT-at-dirpc.nimh.nih.gov, GOLDENSD-at-dirpc.nimh.nih.gov,
"Goldstein, Mark" {GOLDSTEM-at-dirpc.nimh.nih.gov} ,
"Gorey, Julia G." {GOREYJ-at-dirpc.nimh.nih.gov} ,
GRIFFINJ-at-dirpc.nimh.nih.gov, GROSEP-at-dirpc.nimh.nih.gov,
GRUENM-at-dirpc.nimh.nih.gov, HAMIDE-at-dirpc.nimh.nih.gov,
HEATHD-at-dirpc.nimh.nih.gov, HEINZA-at-dirpc.nimh.nih.gov,
"Henter, Ioline" {HENTERI-at-dirpc.nimh.nih.gov} ,
HIRSHMAG-at-dirpc.nimh.nih.gov, HITRIA-at-dirpc.nimh.nih.gov,
"Hodges, Gerald L." {HODGESG-at-dirpc.nimh.nih.gov} ,
HOWARDA-at-dirpc.nimh.nih.gov, HYDET-at-dirpc.nimh.nih.gov,
INNISSS-at-dirpc.nimh.nih.gov, JACKSONS-at-dirpc.nimh.nih.gov,
JAMESD-at-dirpc.nimh.nih.gov,
"James, Johnnie M." {JAMESJ-at-dirpc.nimh.nih.gov} ,
JOHNSONS-at-dirpc.nimh.nih.gov, JONESD-at-dirpc.nimh.nih.gov,
KAROUMF-at-dirpc.nimh.nih.gov, KHAINGZ-at-dirpc.nimh.nih.gov,
KINGJ-at-dirpc.nimh.nih.gov, KLEINMAJ-at-dirpc.nimh.nih.gov,
KNABLEM-at-dirpc.nimh.nih.gov, KOSIOROB-at-dirpc.nimh.nih.gov,
KOW-at-dirpc.nimh.nih.gov, LEEK-at-dirpc.nimh.nih.gov,
LILLRANS-at-dirpc.nimh.nih.gov, LIPSKAB-at-dirpc.nimh.nih.gov,
LIPSKIW-at-dirpc.nimh.nih.gov, LONGR-at-dirpc.nimh.nih.gov,
"Lyons, William E." {LYONSW-at-dirpc.nimh.nih.gov} ,
MASSERAJ-at-dirpc.nimh.nih.gov, MCKIEH-at-dirpc.nimh.nih.gov,
"Meltzer, Marc" {MELTZERM-at-dirpc.nimh.nih.gov} ,
"Merril, Carl R." {MERRILC-at-dirpc.nimh.nih.gov} ,
MILLERG-at-dirpc.nimh.nih.gov, MILLERJ-at-dirpc.nimh.nih.gov,
MILLERM-at-dirpc.nimh.nih.gov, MOTTS-at-dirpc.nimh.nih.gov,
MURRAYA-at-dirpc.nimh.nih.gov, MYSLOBOM-at-dirpc.nimh.nih.gov,
NEVERSA-at-dirpc.nimh.nih.gov,
"Nguyen, Khoi" {NGUYENK-at-dirpc.nimh.nih.gov} ,
OLEARYK-at-dirpc.nimh.nih.gov, PALTANJ-at-dirpc.nimh.nih.gov,
PHILLIPI-at-dirpc.nimh.nih.gov, PLATTH-at-dirpc.nimh.nih.gov,
PODELLD-at-dirpc.nimh.nih.gov, PURCELLC-at-dirpc.nimh.nih.gov,
QUARTEYR-at-dirpc.nimh.nih.gov, RAEDLERT-at-dirpc.nimh.nih.gov,
REDDICKL-at-dirpc.nimh.nih.gov, REIDJ-at-dirpc.nimh.nih.gov,
RIDLEYW-at-dirpc.nimh.nih.gov, RODEFFEC-at-dirpc.nimh.nih.gov,
ROGERSE-at-dirpc.nimh.nih.gov, ROSIERF-at-dirpc.nimh.nih.gov,
RUCKERL-at-dirpc.nimh.nih.gov, RUMBAUGJ-at-dirpc.nimh.nih.gov,
SCHILDTS-at-dirpc.nimh.nih.gov,
"Seals, Troy E." {SEALST-at-dirpc.nimh.nih.gov} ,
SEGALR-at-dirpc.nimh.nih.gov,
"MS:NIHNIMH/DIRPC/SHANNOWC" {SHANNOWC-at-dirpc.nimh.nih.gov} ,
SLADEK-at-dirpc.nimh.nih.gov, SLYDELLP-at-dirpc.nimh.nih.gov,
SMITHE-at-dirpc.nimh.nih.gov, SNITKOVY-at-dirpc.nimh.nih.gov,
SPOORE-at-dirpc.nimh.nih.gov, SRIPATHC-at-dirpc.nimh.nih.gov,
"Staub, Richard A." {STAUBR-at-dirpc.nimh.nih.gov} ,
STEARNSA-at-dirpc.nimh.nih.gov, STICKELD-at-dirpc.nimh.nih.gov,
STOKESA-at-dirpc.nimh.nih.gov,
"Strotkamp, Mollie P." {STROTKAM-at-dirpc.nimh.nih.gov} ,
STURTEVJ-at-dirpc.nimh.nih.gov, SUNDBERL-at-dirpc.nimh.nih.gov,
"Sydnor, Barry C." {SYDNORB-at-dirpc.nimh.nih.gov} ,
"Tolbert, Theresa L." {TOLBERTT-at-dirpc.nimh.nih.gov} ,
"Tompkins, Dera" {TOMPKIND-at-dirpc.nimh.nih.gov} ,
TRAKICA-at-dirpc.nimh.nih.gov,
"Truckenmiller, Emmy" {TRUCKENE-at-dirpc.nimh.nih.gov} ,
TURNERA-at-dirpc.nimh.nih.gov, TURNERM-at-dirpc.nimh.nih.gov,
UNIT2-at-dirpc.nimh.nih.gov, UNIT3-at-dirpc.nimh.nih.gov,
URBINAR-at-dirpc.nimh.nih.gov,
"VanderPuten, Dale" {VANDERPD-at-dirpc.nimh.nih.gov} ,
"Vawter, Marquis P." {VAWTERM-at-dirpc.nimh.nih.gov} ,
VELAF-at-dirpc.nimh.nih.gov, VENABLED-at-dirpc.nimh.nih.gov,
"Waldman, Ivan N." {WALDMANI-at-dirpc.nimh.nih.gov} ,
"Washart, Karen M." {WASHARTK-at-dirpc.nimh.nih.gov} ,
WATSKYE-at-dirpc.nimh.nih.gov, WATSONC-at-dirpc.nimh.nih.gov,
WATSONT-at-dirpc.nimh.nih.gov, WEBSTERM-at-dirpc.nimh.nih.gov,
WEICKERT-at-dirpc.nimh.nih.gov, WEINBERD-at-dirpc.nimh.nih.gov,
WEIRICHM-at-dirpc.nimh.nih.gov, WESCHLEM-at-dirpc.nimh.nih.gov,
WILDERK-at-dirpc.nimh.nih.gov,
"Williams, Jamilah L." {WILLIAJL-at-dirpc.nimh.nih.gov} ,
WILLIAJR-at-dirpc.nimh.nih.gov, WILLIAMJ-at-dirpc.nimh.nih.gov,
WOLFS-at-dirpc.nimh.nih.gov, WOOLFOLJ-at-dirpc.nimh.nih.gov,
WRIGHTR-at-dirpc.nimh.nih.gov, WYATTR-at-dirpc.nimh.nih.gov,
WYNNC-at-dirpc.nimh.nih.gov, YANGF-at-dirpc.nimh.nih.gov,
"Yang, Hsiu-Ying T." {YANGH-at-dirpc.nimh.nih.gov} ,
YATESV-at-dirpc.nimh.nih.gov, "Zullo, Steve" {ZULLOS-at-dirpc.nimh.nih.gov} ,
"Conn, Kathleen" {connk-at-irp.nimh.nih.gov} ,
"Kelly, Dianne" {KellyD-at-odepsm2.od.nih.gov} ,
karau {kuherka-at-badlands.nodak.edu} , iomegafeedback {routh-at-iomega.com} ,
boblinda {103654-at-giga.sct.ub.es} , 1625-at-CompuServe.COM,
arkerlav {arkerlav-at-tigr.org} , avf {avf-at-helix.nih.gov} ,
BLAST-HELP {BLAST-HELP-at-ncbi.nlm.nih.gov} ,
blast {blast-at-ncbi.nlm.nih.gov} , blewis {blewis-at-atcc.org} ,
barbara lewis {blewis-at-helix.nih.gov} ,
"'citron'" {Bob_Citron-at-cc.chiron.com} , bonnie {bonnie-at-codon.nih.gov} ,
bradz {brad-at-codon.nih.gov} , brianm-cmbs {brianm-at-helix.nih.gov} ,
stefanburde {burde-at-lanl.gov} , "Zoltick, Brad J {bz1h} " {bz1h-at-nih.gov} ,
CHANGE {CHANGE-at-nih.gov} , cheri {cheri-at-bgsun2.nimh.nih.gov} ,
Very Merry Cheri Berry {cheris-at-phoenix.princeton.edu} ,
Mike Chipperfield {chipper-at-gdb.org} , circ {circ-at-nih.gov} ,
circadm {circadm-at-nih-library.ncrr.nih.gov} , coolman {Coolman-at-cnn} ,
"CSAR-L%NIHLIST.BITNET" {CSAR-L%NIHLIST.BITNET-at-CU.NIH.GOV} ,
alan {cuaez-at-ecom2.ecn.bgu.edu} , cuaez {cuaez-at-ecom5.ecn.bgu.edu} ,
"Whitley, Ed {cw25f} " {cw25f-at-nih.gov} , dab {dab-at-ray.nlm.nih.gov} ,
sergodani {dani-at-protein.osaka-u.ac.jp} , davidkgdb {davidk-at-gdb.org} ,
"Cohen, Deborah {dc26a} " {dc26a-at-nih.gov} , dec {dec-at-helix.nih.gov} ,
Dale Graham {degraham-at-helix.nih.gov} ,
"Graham, Dale {dg6n} " {dg6n-at-nih.gov} , image-dove {dove-at-uthscsa.edu} ,
ed {edw-at-nchgr.nih.gov} , Leslie Emmert {emmertbk-at-helix.nih.gov} ,
npr-evaluate {evaluation-at-npr.ai.mit.edu} ,
Evgeni Selkov {evgeni-at-mcs.anl.gov} , ffwd2 {ffwd-at-washpost.com} ,
fgsc {fgsc-at-KUHUB.CC.UKANS.EDU} , "5'3'" {fivprime-at-ix.netcom.com} ,
flybrain {flybrain-request-at-gla.ac.uk} ,
"Fr. Jack Frerker" {FR_JACK-at-crc.stmartin.edu} , GXY {GXY-at-CU.NIH.GOV} ,
hackett {hackett-at-focus.nlm.nih.gov} , harbourt {harbourt-at-nlm.nih.gov} ,
hhs {hhsnews-at-dhhs.ssw.dhhs.gov} , HOPOS-L {HOPOS-L-at-UKCC.UKY.EDU} ,
jaxwheeler {hrw-at-aretha.jax.org} , hugo {hugo-at-gdb.org} ,
Ian Clements {IANC-at-probes.mhs.compuserve.com} ,
develsem {idawid-at-nih.gov} , interlibrary {ill-at-nih.gov} ,
tigr-info {info-at-hcd.tigr.org} , imageconsort {info-at-image.llnl.gov} ,
Yalebetsy {jasiorkobl-at-maspo2.mas.yale.edu} ,
pawley {jbpawley-at-facstaff.wisc.edu} , jmb {jmb-at-apuk.co.uk} ,
"'joiner'" {joiner-at-bcm.tmc.edu} , John Pierce {jpierce-at-acf.dhhs.gov} ,
nsf-jporter {jporter-at-nsf.gov} , kevincrowley {kcrowley-at-nas.edu} ,
ken {ken-at-codon.nih.gov} ,
"Kerry.Gascoigne" {Kerry.Gascoigne-at-flinders.edu.au} ,
kshoobridge {kshoobridge-at-lmgvax.nichd.nih.gov} , kt35o-at-nih.gov,
karyn {ku-at-helix.nih.gov} , "Usdin, Karen {ku1j} " {ku1j-at-nih.gov} ,
Kucherla {kucherla-at-aecom.yu.edu} , kurs {kurs-at-novo.dk} ,
"Weeks, Kenneth A {kw1w} " {kw1w-at-nih.gov} ,
"Williams Keith - Exp. Biology" {KWILLIAM-at-eagle.mrc.ac.za} ,
lemkin {lemkin-at-ncifcrf.gov} , lemkin {lemkin-at-fcs280s.ncifcrf.gov} ,
lemkin {lemkin-at-fcs280s.ncifcrf.gov} ,
grossmanl {lg-at-cmb.biosci.wayne.edu} , listproc {listproc-at-soils.umn.edu} ,
IT-list {LISTPROC-at-SPARKY.UTHSCSA.EDU} ,
3dimage {listserv-at-bobcat.etsu.edu} , listbuck {listserv-at-bucknell.edu} ,
LISTSERV {LISTSERV-at-rfmh.org} , DDIRBB-L {Listserv-at-LIST.NIH.GOV} ,
BITNET list server at PUCC {LISTSERV-at-PUCC.PRINCETON.EDU} ,
"'HOPOS-L'" {LISTSERV-at-UKCC.UKY.EDU} , nihlib {LISTSERV-at-ulist.nih.gov} ,
cogsci-L {listserv-at-vm1.mcgill.ca} ,
"'em-argonne'" {LISTSERVER-at-aaem.amc.anl.gov} , lthoja {lthoja-at-uta.fi} ,
majordomo {Majordomo-Owner-at-AmbrosiaSW.com} ,
ambrosia {majordomo-at-AmbrosiaSW.com} ,
conciousness {majordomo-at-lists.zynet.co.uk} ,
qm {majordomo-at-teleport.com} , nelson {manelson-at-pictor.unm.edu} ,
margo {margo-at-jaguar.dote.hu} ,
MayranPE {MayranPE_at_ABD-US-PO3-at-ccip.perkin-elmer.com} ,
Midland primers {mcrc-at-WLN.COM} , science {membership-at-aaas.org} ,
"Carl R. Merril" {merrilcarl-at-msn.com} ,
"'mihales'" {mhp-at-aeolus.nchgr.nih.gov} ,
chipper {Michael.chipperfield-at-sybase.com} ,
Michael OKeefe {Michael_OKeefe-at-macmail7.lbl.gov} ,
Microscopy-request {Microscopy-request-at-Sparc5.Microscopy.Com} ,
microscopy {Microscopy-at-Sparc5.Microscopy.Com} ,
"Macville, Merryn {mm292q} " {mm292q-at-nih.gov} ,
mmaurizi {mmaurizi-at-helix.nih.gov} ,
mark-plate {mmolenda-at-students.wisc.edu} , Molnar {molnarp-at-lib.dote.hu} ,
"Polymeropoulos, Miha {mp139m} " {mp139m-at-nih.gov} , mtd {mtd-at-jax.org} ,
"L.S. SMITH" {MTLLSS-at-ecu-01.novell.leeds.ac.uk} ,
naritsin {naritsin-at-codon.nih.gov} ,
embl {netserv%embl.BITNET-at-CU.NIH.GOV} ,
neuro%emg2 {neuro%emg2-at-emgmhs.mcg.edu} ,
NathanF {nfischel-at-mailgate.csmc.edu} ,
nih-image {nih-image-at-soils.umn.edu} , mnnh {nnh-at-helix.nih.gov} ,
notw {notw-request-at-nine.org} ,
NPR-Mail-Server {NPR-Mail-Server-at-LINCOLN.AI.MIT.EDU} ,
NW Dharma {nwdharma-at-accessone.com} ,
open-meeting {Open-Meeting-at-LINCOLN.AI.MIT.EDU} ,
Laszlo Orban {orban-at-hubi.abc.hu} ,
probes-cs {order-at-probes.mhs.compuserve.com} ,
midlprimers {orders-at-oligos.com} ,
conscious-owner {owner-jcs-online-at-psyche.zynet.co.uk} ,
"'participate'" {participate-at-LINCOLN.AI.MIT.EDU} ,
opmtgpart {participation-at-LINCOLN.AI.MIT.EDU} ,
rogan {pete-at-disomy.peds.hmc.psu.edu} ,
"Lemkin, Peter F {pl1a} " {pl1a-at-nih.gov} , rd {rd-at-zeus.ucsd.edu} ,
doolittle {rdoolittle-at-ucsd.edu} ,
retrieve-help {retrieve-help-at-ncbi.nlm.nih.gov} ,
retrieve {retrieve-at-ncbi.nlm.nih.gov} ,
sallie {RichardS-at-BDG10.NIDDK.NIH.GOV} ,
"Michael H. Rivner, M.D." {rivner%emg2-at-emgmhs.mcg.edu} ,
roganp {rogan-at-ncifcrf.gov} ,
Robert Pearlstein {rpearls-at-hawk.dcrt.nih.gov} ,
"bob's molec models" {rpearls-at-helix.nih.gov} ,
rpearlstein {rpearls-at-helix.nih.gov} , rs137o-at-nih.gov, rs200d-at-nih.gov,
markey {s_markey-at-codon.nih.gov} , saccone {saccone-at-area.ba.cnr.it} ,
easchon {schon-at-cuccfa.ccc.columbia.edu} , sclark {sclark-at-pop.nih.gov} ,
sibbald {sibbald-at-embl-heidelberg.de} ,
sibbald {sibbald-at-qucis.queensu.ca} , istrailprt {sistrai-at-sandia.gov} ,
"'sharon chamberlain'" {slc2-at-aretha.jax.org} ,
"Markey, Sanford P {sm13q} " {sm13q-at-nih.gov} ,
Gary Smejkal {SMEJKAG-at-cesmtp.ccf.org} ,
"SMTP%\"circ-at-nihrrlib.ncrr.nih.gov\"%master.dnet" {"SMTP%circ-at-nihrrlib.ncrr.nih.gov%master.dnet"-at-dxi.nih.gov} ,
"SMTP%\"cuaez-at-ecom2.ecn.bgu.edu\"%master.dnet" {"SMTP%cuaez-at-ecom2.ecn.bgu.edu%master.dnet"-at-dxi.nih.gov} ,
"SMTP%\"degraham-at-helix.nih.gov\"%master.dnet" {"SMTP%degraham-at-helix.nih.gov%master.dnet"-at-dxi.nih.gov} ,
"SMTP%\"SemblyE-at-neon.bprc.nih.gov\"%master.dnet" {"SMTP%SemblyE-at-neon.bprc.nih.gov%master.dnet"-at-dxi.nih.gov} ,
jsnider {sniderjv-at-perkin-elmer.com} ,
SniderJV {SniderJV_at_ABD-US-FLD-at-ccip.perkin-elmer.com} ,
SniderJV {SniderJV_at_ABD-US-PO3-at-ccip.perkin-elmer.com} ,
microworld email {spb-at-wwa.com} , microworld {spignole-at-ix.netcom.com} ,
sheilap {spressman-at-mailgate.csmc.edu} ,
molecmodel {staff-at-hawk.dcrt.nih.gov} ,
"Stephen D. Fuller" {Stephen.Fuller-at-embl-heidelberg.de} ,
goldberg {stgoldberg-at-aol.com} , stromanr {stromanr-at-nih.gov} ,
strzel {strzel-at-speck.niddk.nih.gov} ,
strzel {strzel-at-spider.niddk.nih.gov} ,
"'dani'" {sudani-at-unix.horizontes.com.br} ,
nprsurveys {surveys-at-LINCOLN.AI.MIT.EDU} ,
npreview {surveys-at-town-hall.ai.mit.edu} ,
tanya {tanya-at-imeg.bio.psu.edu} , tberg {tberg-at-u.washington.edu} ,
clontech {tech-at-clontech.com} ,
probes-ts {tech-at-probes.mhs.compuserve.com} ,
Barb Trask {trask-at-biotech.washington.edu} , tried {tried-at-nchgr.nih.gov} ,
"Sargent, Tom (ts1r)" {ts1r-at-nih.gov} ,
twoolf {twoolf-at-ix10.ix.netcom.com} , twoolf {twoolf-at-ix6.ix.netcom.com} ,
twoolf {twoolf-at-ix7.ix.netcom.com} , update {update-at-ncbi.nlm.nih.gov} ,
vaytek {vaytek-at-ins.infonet.net} , verify {verify-at-cnet.com} ,
walker {walker-at-nlm.nih.gov} , wayne {wayne-at-helix.nih.gov} ,
wehart {wehart-at-cs.sandia.gov} , wehart {wehart-at-kiva.cs.sandia.gov} ,
hartprt {wehart-at-sandia.gov} , wilkes {wilkes-at-resgen.com} ,
wrengels {wrengels-at-facstaff.wisc.edu} , engels {wrengels-at-MACC.WISC.EDU} ,
bobzrg {zahr50-at-resgen.com} , zullosun {zullo-at-bgsun2.nimh.nih.gov} ,
zullo {zullo-at-helix.nih.gov}

On Fri, 19 Jul 1996, Zullo, Steve wrote:

}
} Hi,
} My e-mail address will change Monday 22 July 1996 to: {zullo-at-helix-at-nih.gov} .
} Thanks
} Steve
}

It seems it does not work. Please try again...


Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Luis Sole i Sabaris
E-08028 BARCELONA

Tel +34 3 402 16 95
Fax +34 3 402 13 98





From: Nuria Cortadellas :      nuriac-at-giga.sct.ub.es
Date: Mon, 22 Jul 1996 15:19:14 +0000 (GMT)
Subject: Gills

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Does anyone have some experience in gills (Ostrea edulis) fixation for actin
immunolocalization in electron microscopy?.
Thanks in advanced.




From: Jane A. Fagerland (847) 935-0104 :      FAGERLAND.JANE-at-igate.pprd.abbott.com
Date: Mon, 22 Jul 1996 09:22:00 -0500 (CDT)
Subject: Re: LM Tissue Embedding

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Mr-Received: by mta RANDB; Relayed; Mon, 22 Jul 1996 09:31:53 -0500
Mr-Received: by mta MCM$RAND; Relayed; Mon, 22 Jul 1996 09:32:09 -0500
Mr-Received: by mta RANDD; Relayed; Mon, 22 Jul 1996 09:33:24 -0500
Alternate-Recipient: prohibited
Disclose-Recipients: prohibited
Content-Return: prohibited

It sounds to me as if incomplete infiltration is the problem, and the
most likely cause of this is incomplete dehydration. Agarose is
hygroscopic and will pick up water from "anhydrous" reagents that have
taken on water from atmospheric humidity. You may want to add an
additional absolute ethanol step and/or xylene step in your paraffin
processing routine. Also, use fresh ethanol for the last absolute step
and fresh xylene for the last xylene step. Using a desiccant like
"t.h.e Desiccant" in your bottles of solvents will keep them water-free
longer.






From: Jean-Luc Rouviere :      rouvier-at-drfmc.ceng.cea.fr
Date: Mon, 22 Jul 1996 14:43:43 +0200
Subject: Tripode preparation of metallic layers on MgO

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We would like to prepare cross section of metallic multilayers deposited on a MgO substrate using the tripode polishing technique.
We are beginners in the tripode technique and our first attempts were not successfull due to the cleavage of MgO.
Can anybody help us ?




From: Doug Keene :      DRK-at-shcc.org
Date: Mon, 22 Jul 1996 12:46:16 -0800 (PST)
Subject: freezing media for LM

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Fellow Microscopists:

We are interested in embedding a variety of connective tissue matrices, most
from tissues including skin and cartilage, in OCT in preparation for cryostat
sectioning. Our protocol in the past has been to freeze in "hexanes" cooled to
just above its freezing point of -94 C in liquid nitrogen, store in hexanes
at -70C, then embed in OCT and cool to -20 C prior to cryostat sectioning. We
are concerned that there may be a freezing protocol resulting in more favorable
structure. We are concerned that the freeze-thaw-freeze occuring as the tissue
is frozen, then placed in OCT at ambient temp., then frozen again may not be
optimum, even for LM.

Recently, we have considered the use of other freezing media, including
isopentane. We've noticed that some other laboratories are embedding fresh
tissue in OCT, then freezing the entire block in isopentane prior to cryostat
sectioning.

As we make our choice in deciding how to proceed, perhaps others with
experience in freezing tissue in preparation for LM immunocytochemistry might
contribute opinions based on their experience. Our goal is for adequate
stabilization, long term storage of unembeddedd tissue, and good adherence of
tissue to OCT.

Many Thanks,

Doug Keenee





From: Judy Ogilvie :      jmo-at-cidmac.wustl.edu
Date: 22 Jul 1996 16:26:12 +0100
Subject: LM - cryostats

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Message-ID: {n1374069649.72016-at-CIDMAC.wustl.edu}

We are planning to buy a new cryostat soon and would like some input from
current users of newer systems. (We are replacing a 1963 crystat!)
Specifically, we are inclined toward the Zeiss HM505N, but have not found
anyone in St. Louis using one that we could look at or talk to. People using
the Zeiss HM500 can't say enough nice things about it, but it costs about $10K
more than the HM505.

We are a research lab where it will get significant use, but not like in a
path lab where it might be used around the clock. We cut mostly cochleas and
eyecups, both of which are small, but suffer from mixed consistency-- part
boney tissue, part soft tissue, part hollow spaces. Most of our sectioning is
for immunohistochemistry, requiring 6-12 micron sections. St. Louis is a very
humid city, so frost on the blade can be a problem for cryostats.

We are inclined toward a mechanical system since the motorized ones just sound
like one more thing to go wrong. Most of the repairs we have heard about in
newer systems have involved the electronics. Also the vacuum system looks
like another "bell & whistle." Has anyone out there found it to worth an
extra $2-3,000?

Most people we talked to said they liked the cryostat they have, but they
would probably be happy with whatever they got used to using. The only
exception was people from the lab with the Zeiss HM500 who said they liked it
better than anything else they had tried and that they got consistently
bettter sections. So, are there any users of Zeiss HM505's out there? If so,
how do you feel about it?

Thanks in advance for your comments.
jmo






From: saz1-at-ix.netcom.com (Steven Zenk )
Date: Mon, 22 Jul 1996 16:41:42 -0700
Subject: Used SEM

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Dear fellow Microscopists,

I'm looking for a economically priced SEM to be located at a facility
in the Boston area. The SEM would be used for 'general purpose' work,
nothing special. I would like to attach my EDS system to it such that
I can perform x-ray work. Should anyone know of something that might
be available, please contact me.

Thanks for your help!

saz1-at-ix.netcom.com or (415)637-1127 fax




From: geoffa-at-amsg.austmus.gov.au (GeoffA)
Date: Tue, 23 Jul 1996 10:42:44 +1100
Subject: StereoGraphics address?

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Mime-Version: 1.0

Does anyone have email &/or www addresses for Stereographics Corporation
who were in San Raphael, California back in 1993?

Many thanks,

Geoff Avern
Microscopy Labs
Australian Museum
Sydney, Australia




From: ScottE57-at-aol.com
Date: Mon, 22 Jul 1996 23:47:37 -0400
Subject: Re: Subject: Reflected light, DIC, and Kohler Illumination

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David, I am not sure what the setup on your axiplan is but if you have the
reflected light insert you can Kohler in reflected light but most transmitted
light setups would be sold with the fluoresence insert which can not be
kohlered as it lacks an aperture diaphram, you would also need to get the
reflected light polarizer and 50% mirror reflector that into slot where the
fluoresence filter slider may already be, again I would need to know more of
your exact setup to comment, keep in mind that the optics for transmitted
light are usually corrected for a coverslip, reflected light optics generally
are not, this is more of a problem at 40x and higher as optical quality
suffers and I am not sure how DIC quality would be, what are you trying to do
and what exactly is on the microscope.

Scott E. Berman
Advanced Imaging Concepts, Inc.
(609) 921-3629




From: SALLY STOWE :      stowe-at-rsbs-central.anu.edu.au
Date: Tue, 23 Jul 1996 12:53:53 EST10
Subject: LaB6

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Some comments from Roger Heady, who runs the Cambridge S360 here:

We have used LaB6 exclusively for many years, we had some trouble in
the early years but have consistently good results now. Our latest
filament has 1372 hours on the clock and is still first class, not
drifting at all. The aperture was cleaned at about 700 hours.

Some tips - We use Kimball Physics type ES 423E, style 90-20
These have carbon mounts, not tungsten wire.
We use a 1500 micron aperture rather than the 1000 micron as
recommended by Cambridge.
When the filament image starts shifting, clean the aperture with
acid:
1 part conc (36%) HCL to 4 parts water. Immerse, shaking, for 60
sec. Water rinse. Rinse in weal alkali (ammonia or NAOH), water
rinse, alcohol rinse, dry uand use. No polishing.

Set the Kimball tip using an epi-illumination microscope to 125
micron below the front of the aperture plate, and take great care
with the centring.
----------------------------------------------------------------------
Sally Stowe |Email: stowe-at-rsbs.anu.edu.au
Facility Coordinator |Post:
ANU Electron Microscopy Unit |ANUEMU (RSBS)
Ph 61 6 249 2743 |Australian National Univ.
FAX 61 6 249 4891 |Canberra,
http://online.anu.edu.au/EMU/home.htm |AUSTRALIA 0200






From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Tue, 23 Jul 1996 07:49:21 -0700 (PDT)
Subject: Re:freezing media for LM

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Hello Doug, this is Bob Underwood with whom you freeze fractured with at
the University of Wash, in Dr Holbrooks lab.

We routinely place fresh skin samples directly into OCT as soon as
possible and freeze them in either a isopentane or ethanol and dry ice
slush. Isopentane works better. The ethanol can make the OCT turn to
rubber if it comes in contact with it. The blocks are stored in -70
freezer and some have been cut periodicly for 10 years. When using
desposable knives, however, the tissue can detach from surrounding OCT. I
haven't found anything better easier yet. But if you can cool the
isopentane in LN2 it is better but sometimes unavailable.

Bob
underwoo-at-u.washington.edu





From: John Best :      jbest-at-vicon.net
Date: Tue, 23 Jul 1996 10:58:56 -0700
Subject: Wanted: Edax 9900 kb - ISI

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Message-ID: {31F512E0.1C0A-at-vicon.net}

Hi All,

Sorry to ask again, but I really need an EDAX 9900 keyboard! Just hoping
someone out there that didn't see this message previously might know of
someone who's retired their 9900.

Also, I'm interested in a small ISI scope. Miniscan 7, SX-30, SX-40, etc.

Thanks everyone.

Regards,
John Best -- ELMDAS Co.





From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Tue, 23 Jul 1996 10:04:05 -0400 (EDT)
Subject: Re: freezing media for LM

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Hi Doug,

We routinely freeze brain tissue in our EM/Imaging lab. We take fixed
tissue and run it through a sucrose series (10%, 20%, and 30%) to
cryoprotect. We then place the tissue in OCT in a foil cup and place that
on dry ice cooled with acetone. We then store that at -20'c until we
section.

If you would like more details, let me know. We are having pretty good
success.

Cheri Owen
Detroit Neurotrauma Institute
Detroit, Mi 48201
313-285-4027


On Mon, 22 Jul 1996, Doug Keene wrote:

} Fellow Microscopists:
}
} We are interested in embedding a variety of connective tissue matrices, most
} from tissues including skin and cartilage, in OCT in preparation for cryostat
} sectioning. Our protocol in the past has been to freeze in "hexanes" cooled to
} just above its freezing point of -94 C in liquid nitrogen, store in hexanes
} at -70C, then embed in OCT and cool to -20 C prior to cryostat sectioning. We
} are concerned that there may be a freezing protocol resulting in more favorable
} structure. We are concerned that the freeze-thaw-freeze occuring as the tissue
} is frozen, then placed in OCT at ambient temp., then frozen again may not be
} optimum, even for LM.
}
} Recently, we have considered the use of other freezing media, including
} isopentane. We've noticed that some other laboratories are embedding fresh
} tissue in OCT, then freezing the entire block in isopentane prior to cryostat
} sectioning.
}
} As we make our choice in deciding how to proceed, perhaps others with
} experience in freezing tissue in preparation for LM immunocytochemistry might
} contribute opinions based on their experience. Our goal is for adequate
} stabilization, long term storage of unembeddedd tissue, and good adherence of
} tissue to OCT.
}
} Many Thanks,
}
} Doug Keenee
}
}





From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Tue, 23 Jul 1996 16:14:36 -0400 (EDT)
Subject: collagen

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Microscopists:

Is there a secret for embedding ligaments to look at collagen fibers?
do I have to do vacuum infiltration and curing? Oh, by the way, this is
for TEM. In all of my 30 years of doing EM I've never had a real
infilteration problem with any type of tissue until now. I'm goin' nuts.
I had 9 blocks to cut thin sections on and some of the blocks were O.K.
meaning not the best but could get some type of section to look at. After
facing on a Pyramitome, the blocks had a nice,smooth, glassy face.
Some of the blocks seemed to disintigrate as they were being sectioned.
Thicker sections (1.5u) didn't look that bad. Only the thin sections
stank! I'm using Spurr embedding resin which I've never had a problem
with. Maybe the gods of EM weren't smiling on me the day I embedded them.
I did try extending infiltration time over a couple of days. All
chemicals were fresh including alcohols. The only difference is I used ETOH
for my 50:50, etc. I usually use propylene oxide. That was on order and
hadn't come in yet. ETOH is supposed to good with Spurr embedding resin.
Right? At least that's what I was told. I used ETOH in the past with no
problem. Is P.O. better for ligaments? Is there a good procedure for
embedding ligaments? Heeeeeeeeeeeeeeeeeeeeeeelp!

Thanks,

Peace,

Phil 8-{(




From: JBG8NORD-at-aol.com
Date: Tue, 23 Jul 1996 18:07:54 -0400
Subject: TEM Service Subcontractor Needed

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Nordcoff Associates is a discount provider of analytical laboratory services
to semiconductor industry in the U.S. and abroad We currently seek an
independent subcontractor and/or a subcontracting laboratory for TEM analysis
of semiconductors. A variety of capabilities are required, including:

plan-view and crosss-sectional specimen preparation from blanket thin films
and buried layers on mostly Si and GaAs substrates; basic and high resolution
imaging of the films and layers; feature measurements from the images; and
printing / labeling of the images.

We expect a fast turn-around along with dependable and high quality service.

Please respond to me directly. I will provide a summary of the responses to
the interested third parties. Thank you.

Jeff Goldstein, Ph.D.
Norcoff Associates




From: s002swh-at-desire.wright.edu
Date: Tue, 23 Jul 1996 18:02:56 -0500 (EST)
Subject: Re: freezing media for LM

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On Mon, 22 Jul 1996, Doug Keene wrote:

} Fellow Microscopists:
}
} We are interested in embedding a variety of connective tissue matrices, most
} from tissues including skin and cartilage, in OCT in preparation for cryostat
} sectioning. Our protocol in the past has been to freeze in "hexanes" cooled to
} just above its freezing point of -94 C in liquid nitrogen, store in hexanes
} at -70C, then embed in OCT and cool to -20 C prior to cryostat sectioning. We
} are concerned that there may be a freezing protocol resulting in more favorable
} structure. We are concerned that the freeze-thaw-freeze occuring as the tissue
} is frozen, then placed in OCT at ambient temp., then frozen again may not be
} optimum, even for LM.
}
} Recently, we have considered the use of other freezing media, including
} isopentane. We've noticed that some other laboratories are embedding fresh
} tissue in OCT, then freezing the entire block in isopentane prior to cryostat
} sectioning.
}
} As we make our choice in deciding how to proceed, perhaps others with
} experience in freezing tissue in preparation for LM immunocytochemistry might
} contribute opinions based on their experience. Our goal is for adequate
} stabilization, long term storage of unembeddedd tissue, and good adherence of
} tissue to OCT.
}
} Many Thanks,
}
} Doug Keenee
}
Doug,
I have been freezing skin equivalents made of collagen I and III in OCT
(Miles) using isopentane for some time and have found storage of tissue
to be the major obstacle to good histology. If you know someone with
some cryobottle space or a cryofreezer try to store there. Typically my
preps have good overall structure up to 3-4 months at -80, and now about
a year in storage in a cryofreezer.
If you have many different stains to run on the tissue you may want
to section twice as many slides as you think you might need, fix in
methanol or acetone for 10 min. at 4 C and then store the slides at -70
to -80 C. I found this method easier than freezing and thawing my
original tissue each time I wanted to stain for something.
Hope it Helps,
Steve Hendrix
s002swh-at-desire.wright.edu
Rogosin Institute
Ohio Branch }




From: joan.clark-at-sci.monash.edu.au (Joan Clark)
Date: Wed, 24 Jul 1996 14:36:29 +1000
Subject: Help needed from VCE student

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I have received a letter from a VCE student needing some help for her=
extended essay, here is her request -
"I am a year 12 student studying the International Baccalaureate. For my=
extended essay, I have chosed Canine Hip Dysplasia, and require either=
electron micrographs or schematic diagrams of pectineal muscle hypotrophy,=
or any myofibre hypotrophy, showing the difference between type 1 and type=
11 myofibres
Thank you=20
Kristine Batchelor"

If anyone out there can help it would be much appreciated. I will forward=
any information on to her
Regards
Joan Clark






From: joan.clark-at-sci.monash.edu.au (Joan Clark)
Date: Wed, 24 Jul 1996 17:09:34 +1000
Subject: Help needed from VCE student

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} } To:microscopy-at-MSA.microscopy.com
} } From:joan.clark-at-sci.monash.edu.au (Joan Clark)
} } Subject:Help needed from VCE student
} }
} } I have received a letter from a VCE student needing some help for her
} } extended essay, here is her request -
} } "I am a year 12 student studying the International Baccalaureate. For my
} } extended essay, I have chosed Canine Hip Dysplasia, and require either
} } electron micrographs or schematic diagrams of pectineal muscle
} } hypotrophy, or any myofibre hypotrophy, showing the difference between
} } type 1 and type 11 myofibres
} } Thank you
} } Kristine Batchelor"
} }
} } If anyone out there can help it would be much appreciated. I will forward
} } any information on to her
} } Regards
} } Joan Clark
} }
}






From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Wed, 24 Jul 1996 14:54:35 +0100 (BST)
Subject: Going away on Vacation !!

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Please unsubscribe until September 1st 1996
Many thnaks

Patrick







From: Susanne Pignolet Brandom :      spb-at-wwa.com
Date: Wed, 24 Jul 1996 09:36:23 -0500
Subject: Job Posting INSIDE SALES/TECHNICAL SUPPORT

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Message-Id: {2.2.32.19960724143623.00690db0-at-pop.wwa.com}
X-Sender: spb-at-pop.wwa.com
X-Mailer: Windows Eudora Pro Version 2.2 (32)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


} INSIDE SALES/TECHNICAL SUPPORT
}
} Diagnostic Instruments, a 29 yr. old supplier of high quality instruments
} to the international microscope market, is looking for a professional,
} self-motivated person with good communication skills for inside sales and
} technical support. The ideal candidate will possess a BS in Biology,
} Chemistry, or other technical degree. Our rapidly growing company will
} present opportunities for advancement to outside sales and marketing
} positions. Please send or e-mail your resume to:
}
} Diagnostic Instruments
} Attn: Sales Dept.
} 6540 Burroughs Sterling Hts., Mi 48314
} rpatten-at-diaginc.com
Susanne Pignolet Brandom, Ph.D.
MC Services
847-548-6522

MicroWorld Resources and News
http://www.mwrn.com/






From: garyc-at-stud.unit.no (Gary)
Date: Wed, 24 Jul 1996 17:51:26 +0200 (MET DST)
Subject: Microscopy Course

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Does some one know something about courses or conferences covering
Computer-assisted microscopy.


/////////////////////////////////////////////////////////////////
// // //

// Gary Chinga // email :garyc-at-james.avh.unit.no //
// Plantebiosenteret // WWW :http://www.nvg.unit.no/~gary //
// NTNU, 7055 Dragvoll // phone : 73590168 //
// Norway // fax : 73590177 //
// // //
/////////////////////////////////////////////////////////////////






From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Wed, 24 Jul 1996 12:24:28 -0400
Subject: E-mail legalities

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} To: Microscopy List
} From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
} Subject: E-mail legalities
}
} } To: zaluzec-at-sparc5.microscopy.com
} } From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
} } Subject: E-mail legalities
} }
} } } To: zaluzec-at-Microscopy.Com (Nestor J. Zaluzec)
} } } From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
} } } Subject: E-mail legalities
} } }
} } } Nestor,
} } } I am ready to submit the confocal vs deconvolution demo summary, and
} } } am concerned about liabilities. Can I critique company products (we
looked at
} } } five different systems) and reveal our final decision without getting sued?
} } } What are the guidelines and disclaimers to avoid legal harassment? I would
} } } appreciate any info you have or sources I should contact.
} } } Thank you.
} } }
} } } Mike D.
} } }
} }





From: Nate Brinn :      102556.2145-at-CompuServe.COM
Date: 24 Jul 96 13:16:43 EDT
Subject: Subscribe

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From: Glen Macdonald :      glenmac-at-u.washington.edu
Date: Wed, 24 Jul 1996 08:52:21 -0700 (PDT)
Subject: Re: collagen

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Phil,

If infiltration was the problem I'd expect to see something resembling a
rubber erasor or jelly bean. Acetone works better for infiltration than
ethanol, if you need to work without p.o.

We've had ocassional instances of Spurr's shattering like this. The
problem seems to be bad bottles of DMAE. Our policy is to throw out the
DMAE with each empty bottle of DER 736.

Good luck,

Glen MacDonald
Research Scientist
Hearing Research Laboratories of the
Virginia Merrill Bloedel Hearing Research Center
Box 35-7923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu








From: Liang, Long :      LLIANG-at-is.arco.com
Date: 24 Jul 1996 16:21:16 CST
Subject: SEM -- Zeolite source ?

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Message-Id: {MACMS.LLIANG.451242170096206FMACMS-at-IS.ARCO.COM}

Dear Microscopists,

A coworker wants to study zeolites using SEM/EDS. Does anyone know of
any company selling various types of zeolites ?

Does anyone have information about what types of zeolites can be used
for desulfurization of crude oils ?

Thanks in advance.

Long Liang
ARCO EPMA/SEM Lab
Plano, TX






From: robbw-at-ptd.net
Date: Wed, 24 Jul 1996 19:04:52 -0500
Subject: jobs

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I have a friend who is looking for a job in the N. New Jersey area or possibly
the New York City area.

She has a background in TEM with a B.S. in Geology.

She asked me to explore jobs opportunities in the area

Thank you
Sincerely
Robb W





From: generalmicro-at-ccinet.ab.ca (General Microdevices, Inc.)
Date: Thu, 25 Jul 1996 03:44:25 -0600
Subject: TEM - grids/specimen supports

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Hello,

I wish to understand the design and usage of the various grids available
for TEM today, and so I would like to ask if anyone could direct me to
references in the literature that pertain to the grids themselves, and the
methods of preparing them for a specimen, whether the references be for
common or specialized specimen supports, and in any area of TEM
application.

I want to know things like: why they are the way they are (some aspects are
intuitively understandable, but perhaps there are some interesting
subtleties in the reasons); how they are typically used in practice; how
they perform in the TEM (such as how they affect the results); and what the
ideal specimen support in any given application might be. If anyone has
information related to these questions that is not embodied in a published
source, I would be happy to learn of it, too.

Cheers,

Cam

____________________________________________________________________________
Box 1932 Main Station T: 1 403 435 2167
Cameron Sorlie, President Edmonton, AB T5J 2P3 Canada F: 1 403 433 9376
General Microdevices, Inc. -----------------------------------------------
generalmicro-at-ccinet.ab.ca
______________________________________________________
Microtechnology products for science and industry






From: chris gilpin :      CGILPIN-at-fs1.sem.man.ac.uk
Date: Thu, 25 Jul 1996 11:10:38 BST
Subject: image intensified cameras for TEM

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Dear All
I am looking for an image intensified camera to fit a Philips
400/420. Does anyone have one that they don't use and would be
willing to sell/donate

Many thanks

Chris


Chris Gilpin
Biological Sciences Electron Microscope Unit
G452 Stopford Building
Oxford Road
Manchester
M13 9PT
phone +44 161 275 5170
fax +44 161 275 5171




From: Koenraad.Janssens-at-mtm.kuleuven.ac.be (Koenraad Janssens)
Date: Thu, 25 Jul 1996 13:26:00 +0200
Subject: SIMCON

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Dear Fellow Microscopists,

The last few years I have been involved in research on localized (near
nanometer resolution) strain characterization using two-beam electron
diffraction contrast imaging (an operational mode of transmission
electron microscopy). See any book on transmission electron microscopy
for the basics.

Part of this project was to develop a software package allowing for the
analysis of strain fields of arbitrary geometry. This software (SIMCON)
allows one to model a microscopic strain field with various mathematical
methods (including finite elements) and subsequently verify this model
by comparison with experimental observations in the TEM.

As of today we are releasing SIMCON as freeware, you can find out all
details at the following address:

http://www.mtm.kuleuven.ac.be/~simcon/

Friendly Greetings,

Koen Janssens

___________________________________________________________________ _ _ _
___________________________________________________________________ _ _ _

Koenraad Janssens, Ph.D.

KULeuven
Department of Metallurgy and Materials Engineering (MTM)
de Croylaan 2, B-3001 Leuven, Belgium
Tel. : +32-(0)16-32.1232
Fax : +32-(0)16-32.1992
e-mail : Koenraad.Janssens-at-mtm.kuleuven.ac.be
www : http://www.mtm.kuleuven.ac.be/Members/Researchers/KoenraadJanssens/


!!! New address valid from 1 September 1996 !!!

OCAS
John F. Kennedylaan 3, B-9060 Zelzate, Belgium
Tel. : +32-9-345.12.11 (OCAS reception)
Fax. : +32-9-345.12.04




From: gllovel-at-ppco.com (Gary Lovell)
Date: Thu, 25 Jul 1996 13:48:40 -0500
Subject: Ternary Plots

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X-Nupop-Charset: English

I am currently using DeltaGraph Professional for ternary plots of pyroxene endmember compositons. The software does not allow one to change the axis scale, thus tighly grouped points on a plot are unsightly at best. Is anyone aware of a softwar
e program that will run in Windows 95 allowing one to manipulate the ternary plot axis?





From: EMLAB-at-vet.ksu.edu
Date: Thu, 25 Jul 1996 14:53:06 CST6CDT
Subject: data management

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We are beginning to look at a new computer and software. (The 286
just can't keep up!) Can anyone suggest software or strategies to
record and manage data; ie cutomers, case ID, inventory, billing, grid
location, etc.?

We would like to be able to find all information that we already have
for a specific animal/tissue when they come in, for comparative
reasons.

ka





From: SilverStf-at-aol.com
Date: Thu, 25 Jul 1996 17:52:35 -0400
Subject: phosphor powder/Luminescent mat'l

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Hi Microscopists:

Does anyone know where I can purchase some phosphor powder? My last batch
was from Levy West Laboratories Enfield and was purchased in 1978. It is
marked "Luminescent material" and "Type 4CS70F". Does Levy West still exist
and if so does anyone have a phone or fax number. Is this type of phosphor
still available somewhere???? Thanks for any help you can give me.

Anne Esposito
E.M. Connection
e-mail: Silverstf-at-aol.com




From: robbw-at-ptd.net
Date: Thu, 25 Jul 1996 15:00:32 -0500
Subject: line TX speeds

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When I use my modem for an internet connection my TX speed is about 1K/sec ave
rate.
Does anyone have insight into the reason for this speed reduction?
My modem is 14.4K

Thank you
Robb
=============================================
\\\\|////
| ~ ~ |
|(-at-)-(-at-)| Have a Great Day!
| 0 | /
| [___] |
ooo0---- { } ---0ooo-------------------------------
/*\ Robb Westby NORAN Instruments
/*//\ 800.691.4610 voice mail pager
\//*/ 608.828.4428 voice mail box
\*/ 608.831.4461 fax
internet robbwestby-at-noran.com
http:\\www.noran.com
-----------------------------------------------




From: pat_masarachia-at-merck.com (Pat Masarachia)
Date: Thu, 25 Jul 1996 16:37:18 EST
Subject: polysacharide structure and TEM

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Message-Id: {199607252252.SAA19176-at-igw2}

I have TEM experience only with cells and tissue. Someone has asked me
if I could show the structure of a pneumococcal polysacharide using TEM.
I understand that proteins, DNA and viruses are studied using negative
staining. Does anyone have information about the possibility of seeing
a sugar molecule for the purpose of comparing two structural forms of it?
Is there a negative stain? Thanks for any comments.
Pat Masarachia
e-mail pat_masarachia-at-merck.com
Merck Research Labs
West Point, Pa 19486
phone 215-652-7999






From: roy-at-bayou.uh.edu (Roy Christoffersen)
Date: Thu, 25 Jul 1996 16:25:44 -0500
Subject: Diffraction contrast simulations with MaComis

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Regarding the recent postings about diffraction contrast simulations, one
of the more recently developed contrast simulation programs around is
MaComis, developed by Rene Rasmussen at Comisoft. It has a user friendly
Macintosh interface, but I don't think it has the several advanced features
that Alwyn Eades is looking for. I have an advanced beta version of the
program but I am not sure if it ever made it out of beta and into the
"marketplace". I have Comisoft's address as of early 1993, but I don't know
if Rene is still running it or has moved on to other places and things. I
would be happy to pass on my information regarding about MaComis and
Comisoft to whomever is interested. However, I have no personal or
commercial connection to McComis or Comisoft.

Roy Christoffersen
Texas Center for Superconductivity
3201 Cullen
Houston, TX 77204-5932
roy-at-bayou.uh.edu
(713) 743-8273
FAX: (713) 743-2787






From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Thu, 25 Jul 1996 19:03:23 -0500
Subject: Re: line TX speeds

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Message-Id: {199607260003.TAA04950-at-mailhub.iastate.edu}
X-Sender: wes-at-pop.ameslab.gov
X-Mailer: Windows Eudora Pro Version 2.1.2
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

A baud is one bit per second. A data byte is about 10 bits (7 or 8 data plus
some start and stop bits).

Therefore, 1 K-byte per second is about 10 k-bit per second or 10K baud. And
remember you have to leave some room for acknowledgements from the other
end, response delays, etc. You actually seem to be doing pretty well.

At 03:00 PM 7/25/96 -0500, you wrote:
}
} When I use my modem for an internet connection my TX speed is about 1K/sec ave
} rate.
} Does anyone have insight into the reason for this speed reduction?
} My modem is 14.4K
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Thu, 25 Jul 1996 18:00:26 -0500
Subject: Deadline for Late Breaking Posters for Microscopy & Microanalysis - 96

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To: Microscopy Listserver Subscribers & MSA Members

Re: Late Breaking Poster Session for Microscopy & Microanalysis 96
August 11-15, 1996 Minneapolis, Minnesota


The deadline for receipt of abstracts for the Late Breaking Posters
session of the Microscopy & Microanalysis -96 Meeting was
set to be Friday, July 26 .

As I have completed processing of all the received applications, I
have decided to extend the deadline a few more days to allow any
individuals still interested in submitting a late breaking poster a last
chance to present their results at the meeting. I will still
accept applications and abstracts through 5pm local time next Tuesday
(July 30th). You will be informed by Thursday August 1 of poster acceptance
or rejection.

Information on submission is given below as well as on the
Microscopy Society of America WWW Site

http://www.msa.microscopy.com

Nestor J. Zaluzec
M&M 96 Program Chairman

=========================================================


Late Breaking Poster Session Information



To submit a late breaking poster you must fill out a Data Form (available
via the WWW http://www.msa.microscopy.com ) and prepare an
Abstract as described in the Call for Papers ( or contact
the Microscopy & Microanalysis office). Express Mail the completed
Data Form and Abstract to:

Dr. Nestor J. Zaluzec, Program Chair
Microscopy & Microanalysis '96
797 Bonnie Brae Ct
Bolingbrook, IL 60440
USA

After your submission is reviewed, you will be contacted about acceptance
and with
instructions on when and where to display your
poster. The EXTENDED deadline for receipt is: Tuesday JULY 30, 1996 5 pm
local time.


Although Abstracts accepted as Late Breaking Posters cannot be published
in the Proceedings, they will be listed on a special Meeting
handout and in daily Meeting newsletters.







From: Visit Thaveeprungsriporn :      fntvtv-at-eng.chula.ac.th
Date: Fri, 26 Jul 1996 15:07:03 +0700 (TST)
Subject: SEM - SACP Analysis

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Does anyone know of public or commercial softwares (PC) that analyze
SEM-based selected area channeling pattern (SACP)?

Visit Thaveeprungsriporn
Chulalongkorn University






From: Mnr HJ Els :      HJELS-at-op1.up.ac.za
Date: Fri, 26 Jul 1996 10:17:58 GMT+2
Subject: duodenal brush border vesicles

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Hello all subscribers,
Duodenal brush border vesicles - DBBV- and negative staining.
We do negative staining of duodenal content of ostriches regularly as
well as faeces samples of dogs, calves, etc to detect the
presence of any possible virus particles eg. parvo, corona, picorna,
rota, etc. However we experience some difficulties in diagnosing
paramyxovirus (orthomyxo?) particles beyond doubt. The problem arises
when we encounter paramyxovirus-like particles from low numbers to
masses of them in a specimen BUT no sign of any helical nucleoprotein
strands. How can we break up these particles to release any
nucleocapsid strands if they are virus particles?
We centrifuge 15ml suspension of samples at low speed (3000g, 15min)
and then supernatant at high speed (20000g, 60min) and perform
neg. staining on pellet as standard procedure.
I have one reference on DBBV: Arch Virol 1987, 97:309-323 by Schnagl
RD. et al but their one small micrograph is not adequate to compare
with myxoviruses.
Can anyone supply me with a micrograph of x100-150K magnification of
DBBV (or any other reference) to compare with our own known
micrographs of paramyxoviruses or give info on how to obtain DBBV
easily(?) for negative staining.
Q: What happens to the duodenal epithelial lining during a viral
(bacterial) infection; can it shred structures (vesicles) in such vast
numbers and do they have a viral-like appearance with negative
staining?
Any suggestions would be appreciated very much.
Friendly greetings,
Hercules Els
EM Unit, Fav Vet Sci, Univ of Pretoria, Onderstepoort 0110 Rep of
South Africa
hjels-at-op1.up.ac.za










From: Joe D Geller :      geller-at-world.std.com
Date: Fri, 26 Jul 1996 08:35:33 -0400 (EDT)
Subject: Mitutoyo Microscopes

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I would appreciate information leading to the availability of a pre-owned
Mitutoyo FS60 microscope.

Joe Geller
Geller MicroAnalytical Lab
508 887-7000 fax: 508 887-6671






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Fri, 26 Jul 1996 09:11:04 -0400
Subject: Re: polysacharide structure and TEM

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} I have TEM experience only with cells and tissue. Someone has asked me
} if I could show the structure of a pneumococcal polysacharide using TEM.
} I understand that proteins, DNA and viruses are studied using negative
} staining. Does anyone have information about the possibility of seeing
} a sugar molecule for the purpose of comparing two structural forms of it?
} Is there a negative stain? Thanks for any comments.
} Pat Masarachia
} e-mail pat_masarachia-at-merck.com
} Merck Research Labs
} West Point, Pa 19486
} phone 215-652-7999
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
Negative staining might give you some information. Just start trying the
different ones and see how they look. Low angle metal shadowing of a thin
dispersion of the material on a carbon or formvar coated grid should also
show some structure or you may need to go alll the way to making carbon-Pt
replicas.

If you could get access to a Zeiss energy filtering instrument with a cryo
holder you might be able to view it in thin films of vitreous ice.
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: skperkin-at-mail.vt.edu (sandra perkins)
Date: Fri, 26 Jul 1996 10:43:07 -0500
Subject: unsubscribe

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From: NORM OLSON :      NHO-at-bragg.bio.purdue.edu
Date: Fri, 26 Jul 1996 10:57:36 -0500 (EST)
Subject: Re: polysacharide structure and TEM

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{} I have TEM experience only with cells and tissue. Someone has asked me
{} if I could show the structure of a pneumococcal polysacharide using TEM.
} } I understand that proteins, DNA and viruses are studied using negative

{Negative staining might give you some information. Just start trying the

{If you could get access to a Zeiss energy filtering instrument with a cryo
{holder you might be able to view it in thin films of vitreous ice.

A GOOD negative stain prep is the way to start but
vitreous ice and a cryo holder, as the above author suggests, is
definitely the way to go if you can manage it. Although contrast
with cryo is much less than that obtained with negative stain you
don't generally have to worry about the effects of drying.
I would, however, suggest a different scope than a Zeiss. Some of
the other scopes on the market have a much better track record
with cryo.

Norm Olson





From: gllovel-at-ppco.com (Gary Lovell)
Date: Fri, 26 Jul 1996 12:39:04 -0500
Subject: Ternary Plots

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Sorry!! I am resending this message because something was incorrect in my Eudora configuration and I did not receive the replies. I would appreciate hearing again from the people that did respond.

????????????????????????????????????????????????????????????

I am currently using DeltaGraph Professional for ternary plots of pyroxene endmember compositons. The software does not allow one to change the axis scale, thus tighly grouped points on a plot are unsightly at best. Is anyone aware of a softwar
e program that will run in Windows 95 allowing one to manipulate the ternary plot axis?





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 26 Jul 1996 14:52:16 -0400
Subject: Diff Pmp Heaters

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Message-ID: {n1373729619.33990-at-mse.engin.umich.edu}

Subject: Time: 2:41 PM
OFFICE MEMO Diff Pmp Heaters Date: 7/26/96

Some time ago someone asked about where to buy diffusion pump heaters. I
have just come across a good source, and am giving it here for everyone who
may have an interest in such info. It is:
Dalton Electric Heating Co.
28 Hayward Street
Ipswich, MA 01938-9978
Dalton advertises producing over 100 standard types of heaters, and a
willingness to make custom heaters for obsolete or foreign-made pumps.





From: robbw-at-ptd.net
Date: Fri, 26 Jul 1996 11:04:46 -0500
Subject: Re: data management

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I also am interested in this information

Thank you

On Thu, 25 Jul 1996, EMLAB-at-vet.ksu.edu wrote:
} We are beginning to look at a new computer and software. (The 286
} just can't keep up!) Can anyone suggest software or strategies to
} record and manage data; ie cutomers, case ID, inventory, billing, grid
} location, etc.?
}
} We would like to be able to find all information that we already have
} for a specific animal/tissue when they come in, for comparative
} reasons.
}
} ka
}
}
}
}
=============================================
\\\\|////
| ~ ~ |
|(-at-)-(-at-)| Have a Great Day!
| 0 | /
| [___] |
ooo0---- { } ---0ooo-------------------------------
/*\ Robb Westby NORAN Instruments
/*//\ 800.691.4610 voice mail pager
\//*/ 608.828.4428 voice mail box
\*/ 608.831.4461 fax
internet robbwestby-at-noran.com
http://www.noran.com
-----------------------------------------------




From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Fri, 26 Jul 1996 15:40:00 -0600
Subject: Re: Ternary Plots

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Hi, Gary,

Yes, DeltaGraph Prof is capable to change the axis scale for
ternary diagram. The procedure for this is

a. active the figure that you want to modify
b. go to Chart (manu), then Axis, Value, Axis Attributes
C. assign length and units for axis

} I am currently using DeltaGraph Professional for ternary plots of
} } pyroxene endmember compositons. The software does not allow one to
} change the } axis scale, thus tighly grouped points on a plot are unsightly
} at best. Is } anyone aware of a softwar

Xiaogang

***********************************
* Xiaogang Xie *
* SEM & Microprobe lab *
* Dept. of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
***********************************






From: taylor-at-iris1.sb.fsu.edu (Kenneth A. Taylor)
Date: Fri, 26 Jul 1996 19:23:00 -0600
Subject: Postdoctoral position

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Postdoctoral position available immediately to study the 3-D structure of
insect flight muscle. The research project, which is funded through 1999,
offers a unique opportunity to correlate 3-D electron microscopy,
mechanics, X-ray diffraction and atomic modeling of different physiological
states including AMPPNP and AMPPNP-glycol treated muscle,
freeze-substituted quick-frozen contracting muscle, Drosophila wild-type
and mutant flight muscle, relaxed muscle, etc. Several experimental
approaches including electron microscope tomography and oblique section
reconstruction for producing 3-D images, alignment and classification of
3-D crossbridge structures and fitting of atomic coordinates of actin and
myosin S1 into the envelope of 3-D images obtained by 3DEM. The position
involves primarily computer processing of electron micrographs and
molecular modeling. Salary dependent on years of relevent postdoctoral
experience.

Our laboratory is part of the Structural Biology Program at Florida State
University. The Institute of Molecular Biophysics is strategically located
between the Biology and Chemistry Buildings. Abundant opportunities are
available to develop new collaborations and investigate new scientific
questions. For more information check out the institutes web page at
http://www.sb.fsu.edu. Our laboratory has Silicon Graphics workstations
and a Perkin-Elmer PDS 1010M microdensitometer. Electron microscope
facilities include a Philips CM300-FEG and CM120 electron microscopes
equipped with Gatan and Oxford Instruments cryostages. Interested
applicants should send their CV and names, addresses and phone numbers of 3
references to Dr. Kenneth A. Taylor, Institute of Molecular Biophysics,
Florida State University, Tallahassee, FL 32306-3015, USA. My E-mail
address is taylor-at-sb.fsu.edu. Phone number 1-904-644-3357, FAX
1-904-561-1406.


{ {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } {

Kenneth A. Taylor, Ph.D. Phone: 904-644-3357
Institute of Molecular Biophysics Fax: 904-561-1406
Florida State University E-mail: taylor-at-sb.fsu.edu
Tallahassee, FL 32306-3015
Home pages: http://www.sb.fsu.edu/~taylor/
http://www.fsu.edu/~biology/faculty/kat.html

{ {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } {








From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Sat, 27 Jul 1996 08:21:11 +0000
Subject: Re: polysacharide structure and TEM

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Microscopy-at-Sparc5.Microscopy.Com

Just to follow up on my previous comments, and to add to a couple of the
others, some work has been done on staining of cryo-fixed specimens, which
helps with the low contrast of cryo-fixed material. I can't remember the
details of the technique - I guess you actually have to stain the specimen
before cryo-fixation - but I think that a tin compound is used.

Some type of electron spectroscopic imaging would also help with the
contrast, the options are either a bolt-on imaging spectrometer, such as
the one Gatan produce, or the in-coloumn energy filter. Until recently,
Zeiss were the only option here. However, Philips have just launched a
version of their CM120 which has an in-column filter. I'm sure they would
like some nice applications problems for promoting it - I'd give them a
call and see if you can get some free microscope time!

Regards

Larry Stoter






From: Hans-Martin Vaihinger :      Hans-Martin.Vaihinger-at-rz.ruhr-uni-bochum.de
Date: Sat, 27 Jul 1996 13:12:22 +0000
Subject: LR White embedding

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Comments: Authenticated sender is {vaihihbt-at-mailhost.rz.ruhr-uni-bochum.de}

Dear Microscopists

We are only just beginning to use LR White as embedding medium for
use in EM- immunocytochemistry. A manual by AGAR SCIENTIFIC LTD.
distributed through PLANO recommends to bring the specimen from
70 % EtOH into LR White/70 % EtOH (2:1) before embedding in pure LR
White. We find that this mixture very quickly separates into two
phases. How shall we handle this?
Furthermore the specimen seems to be cured insuffiently even after
24 hours of polymerisation at 55 C. Any comments on this?

Thanks for any inputs

**************************************************************
Hans-Martin Vaihinger
Ruhr-University of Bochum
Comparative Endocrinology Research Section
Building ND 5/37
44780 Bochum
GERMANY
*********************************************************
phone ++49 234 700 4329
fax ++49 234 709 4551
email Hans-Martin.Vaihinger-at-RZ.Ruhr-Uni-Bochum.de




From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Sun, 28 Jul 1996 13:25:34 -0400 (EDT)
Subject: collagen

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I would like to thank all of you who responded about my problem with the
infiltration of ligaments. It's good to here that others are having
problems with the Spurr embedding kits. I used fresh chemicals and this
was the first problem I've ever encounterd using this resin. Araldite
502 has never let me down, maybe I'll continue to use 502 and wait until
the problems are sorted out with Spurr's embedding resin. Again.....
THANKS!

PEACE,

PHIL




From: Smith, Peter :      SMithP-at-agresearch.cri.nz
Date: Mon, 29 Jul 1996 09:15 +1200 (NZST)
Subject: Data Management

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We have used microsoft access for a number of years and have found it to be
excellent for data management, storage of block information, etc etc.Its
compatibility with other software packages also makes it useful, its worth
having a look at.

regards Peter Smith
AgResearch Wallaceville
NZ




From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Mon, 29 Jul 1996 10:49:24 +1100
Subject: Re: LR White embedding

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} Dear Microscopists
}
} We are only just beginning to use LR White as embedding medium for
} use in EM- immunocytochemistry. A manual by AGAR SCIENTIFIC LTD.
} distributed through PLANO recommends to bring the specimen from
} 70 % EtOH into LR White/70 % EtOH (2:1) before embedding in pure LR
} White. We find that this mixture very quickly separates into two
} phases. How shall we handle this?
} Furthermore the specimen seems to be cured insuffiently even after
} 24 hours of polymerisation at 55 C. Any comments on this?

The 70% EtOH needs to be made up freshly just before use. Even stored in a
tightly stoppered bottle, the concentration of the EtOH seems to decrease.
This solved my similar problems.

Diana van Driel
Dept Ophthalmology
Sydney University C09
AUSTRALIA 2006






From: Bob Nunes Cardozo :      b.nunescardozo-at-ioi.knaw.nl
Date: Mon, 29 Jul 1996 11:21:18 MET
Subject:

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subscribe microscopy




From: Joe D Geller :      geller-at-world.std.com
Date: Mon, 29 Jul 1996 09:40:34 -0400 (EDT)
Subject: Balzers TMP Controller

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We have need for a pre-owned Balzers TCP-300, 310 or 380 controller with a
cable for a Balzers 170 l/s TMP.

Would appreciate any leads.

Joe Geller
508 887-7000





From: FRANKS-at-inland.com
Date: Mon, 29 Jul 1996 09:46:07 -0500 (CDT)
Subject: ISO or A2LA -- EPMA lab certification

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As part of our organization's efforts to attain ISO9000 certification, I will
soon be faced with the task of preparing our electron microprobe laboratory
for a preliminary audit.

I would like to correspond with anyone who has been throught this experience.


Larry Franks
Franks-at-Inland.com




From: Sara Miller :      saram-at-acpub.duke.edu
Date: Mon, 29 Jul 1996 11:10:01 -0400 (EDT)
Subject: RE: LM Tissue Embedding

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On Fri, 19 Jul 1996 s002swh-at-desire.wright.edu wrote:

} Date: Fri, 19 Jul 1996 19:12:30 -0500
} From: s002swh-at-desire.wright.edu
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: RE: LM Tissue Embedding
}
} APB,
} I'm trying to fix, dehydrate and embed in paraffin, cells in a
} suspension of collagen and agarose. The agarose is giving me fits. I'm
} trying different concentrations of formalin, and have tried xylene and
} ethlyne glycol as transitions to wax. Two problems are occurring. 1) the
} center of the tissue is not embedding. 2) When sectioning the agarose
} shears and comes out of the paraffin.
} Any suggestions would be helpful,
} TIA
}
} Steve Hendrix
} Wright State University
} s002swh-at-desire.wright.edu
}
Infiltration into cells that have been embedded in agar and then
aldehyde-fixed is difficult to impossible.
We don't do wax, rather epoxiy and acryllic for electron
microscopy and have run into this problem. We get around it by fixing
the cells in glutaraldehyde, pelleting them, then encasing them in agar,
and finally going through the other fixes and dehydrants.} }
}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: maginfo-at-oxford.usa.com (MAG US SALES INFO)
Date: Mon, 29 Jul 1996 11:10:01 -0400 (EDT)
Subject: Job Opportunity

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date: Mon, 29 Jul 96 11:30
message-id: {amajdbcc-at-oxford.usa.com}


---- WDS Applications Specialist ---

Oxford Instruments, Microanalysis Group, a leading manufacturer of x-ray
microanalysis instrumentation, has an immediate opening for an Applications
Engineer experienced in Wavelength Dispersive X-ray Spectrometry to join our
support team. This exciting position is based in our West Coast facility in
Fremont, CA.

The varied role will provide: customer demonstrations and applications,
customer training, technical support of the sales teams, product testing and
all other aspects of effective customer support. Some domestic and foreign
travel will be required.

The successful candidate will have a minimum of 2 years experience in
wavelength dispersive spectrometry (microanalysis or XRF) and a degree in
the physical sciences. Excellent verbal and written communication skills,
and a professional, outgoing personality are a must. Additional experience
working with SEMs, EDS analysis, PCs and Microscopt Windows is desirable.

Please contact hr-at-ca.oxford.usa.com for an application or fax your resume
to:

Oxford Instruments, Inc.
Microanalysis Group
Attn: Marketing Dept.
45950 Hotchkiss Street
Fremont, CA 94539
Fax: 510/656-8944

NO PHONE CALLS PLEASE.





From: Cliff Priebe :      cliff-at-hal-pc.org
Date: Mon, 29 Jul 1996 13:03:33 +0000
Subject: (no subject)

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Message-ID: {31FCB6A5.3D02-at-hal-pc.org}

subscribe microscopy

cliff-at-hal-pc.org



Cheers,

Cliff




From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 7/27/96 3:43 PM
Subject: data management

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We are beginning to look at a new computer and software. (The 286
just can't keep up!) Can anyone suggest software or strategies to
record and manage data; ie cutomers, case ID, inventory, billing, grid
location, etc.?

We would like to be able to find all information that we already have
for a specific animal/tissue when they come in, for comparative
reasons.

ka






From: Sara Miller :      saram-at-acpub.duke.edu
Date: Mon, 29 Jul 1996 13:22:53 -0400 (EDT)
Subject: Re: TEM - grids/specimen supports

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On Thu, 25 Jul 1996 generalmicro-at-CCINET.AB.CA wrote:

} Date: Thu, 25 Jul 1996 03:44:25 -0600
} From: generalmicro-at-CCINET.AB.CA
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: TEM - grids/specimen supports
}
}
} Hello,
}
} I wish to understand the design and usage of the various grids available
} for TEM today, and so I would like to ask if anyone could direct me to
} references in the literature that pertain to the grids themselves, and the
} methods of preparing them for a specimen, whether the references be for
} common or specialized specimen supports, and in any area of TEM
} application.
}
} I want to know things like: why they are the way they are (some aspects are
} intuitively understandable, but perhaps there are some interesting
} subtleties in the reasons); how they are typically used in practice; how
} they perform in the TEM (such as how they affect the results); and what the
} ideal specimen support in any given application might be. If anyone has
} information related to these questions that is not embodied in a published
} source, I would be happy to learn of it, too.
}
} Cheers,
}
} Cam
}
} ____________________________________________________________________________
} Box 1932 Main Station T: 1 403 435 2167
} Cameron Sorlie, President Edmonton, AB T5J 2P3 Canada F: 1 403 433 9376
} General Microdevices, Inc. -----------------------------------------------
} generalmicro-at-ccinet.ab.ca
} ______________________________________________________
} Microtechnology products for science and industry
}
Cam,
}
There is information on grids, support films, preparation/use of, etc. in
"Negative Staining" by MA Hayat and SE Miller, Mc Graw-Hill Publishing Co.,
Hew York, 1990.


Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: Sara Miller :      saram-at-acpub.duke.edu
Date: Mon, 29 Jul 1996 11:47:32 -0400 (EDT)
Subject: Re: collagen

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On Tue, 23 Jul 1996, rutledge phil wrote:

} Date: Tue, 23 Jul 1996 16:14:36 -0400 (EDT)
} From: rutledge phil {prutle1-at-gl.umbc.edu}
} To: microscopy {Microscopy-at-Sparc5.Microscopy.Com}
} Subject: collagen
}
} Microscopists:
}
} Is there a secret for embedding ligaments to look at collagen fibers?
} do I have to do vacuum infiltration and curing? Oh, by the way, this is
} for TEM. In all of my 30 years of doing EM I've never had a real
} infilteration problem with any type of tissue until now. I'm goin' nuts.
} I had 9 blocks to cut thin sections on and some of the blocks were O.K.
} meaning not the best but could get some type of section to look at. After
} facing on a Pyramitome, the blocks had a nice,smooth, glassy face.
} Some of the blocks seemed to disintigrate as they were being sectioned.
} Thicker sections (1.5u) didn't look that bad. Only the thin sections
} stank! I'm using Spurr embedding resin which I've never had a problem
} with. Maybe the gods of EM weren't smiling on me the day I embedded them.
} I did try extending infiltration time over a couple of days. All
} chemicals were fresh including alcohols. The only difference is I used ETOH
} for my 50:50, etc. I usually use propylene oxide. That was on order and
} hadn't come in yet. ETOH is supposed to good with Spurr embedding resin.
} Right? At least that's what I was told. I used ETOH in the past with no
} problem. Is P.O. better for ligaments? Is there a good procedure for
} embedding ligaments? Heeeeeeeeeeeeeeeeeeeeeeelp!
}
} Thanks,
}
} Peace,
}
} Phil 8-{(
}
I don't know anything about ligaments, so I may be all wet, but we've cut
some pretty rubbery stuff. I can answer only some of your questions.
Yes, ethanol works fine with Spurr. After a graded series, we use 95% 2
X, then 100% 3 X. The secret is that the ethanol must be DRY. If it's
not a new bottle, you should put in some drying beads (molecular sieves,
made of diatomaceous earth, I think). We leave the beads in the bottle,
and every time we empty it ~200 ml), we pour out the beads and bake
them. CAUTION: Make sure the ethanol has evaporated completely before
baking, or the whole lot will explode, sending beads everywhere!
(Experience speaking.) One variable I can't comment on is the length of
time in each change. We use 10 min for cell monolayers and 20-30 min for
tissues and agar-embedded cells. You might want to try a little longer,
if you're having trouble with dehydration. Then we use the anhydrous
ethanol mixed 50:50 with resin, followed by 100 % resin 2 X. Again, the
time will vary with the tissue. For cells, we use 30 min, and for
tissues, 30-60 min, depending on the tissue and its size.

To salvage your already-embedded tissue, you might want to bake it some
more (another 3-6 hr), try cutting a little thicker sections (100-120 u),
and picking them up on Formvar-coated grids. If the sections fall apart
in the boat after floating a while, cut only 1 or 2 sections and then pick
them up on film-coated grids. Finally, carbon-coat the sections in a
vacuum evaporator to stabilize them in the beam.

Good luck.
Sara

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: jan_ringnalda-at-pei.philips.com (Jan Ringnalda)
Date: 7/27/96 8:21 AM
Subject: Re: polysacharide structure and TEM

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Mime-Version: 1.0
Larry Stoter {LPS-at-teknesis.demon.co.uk}
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
Content-Description: cc:Mail note part

Dear All,
The information Larry gives is not quite correct; Philips have
announced the launch of a "Biofilter" microscope, however it is not an
in-column filter. For those of you interested please attend the
Philips booth at both Eurem and MSA for demo's and "free microscope
time". Suffices to say that the concept is quite revolutionary!

Sincerely,
Jan Ringnalda,
Sr. Application Specialist,
Philips Electron Optics, Mahwah, NJ 07430


______________________________ Reply Separator _________________________________


Just to follow up on my previous comments, and to add to a couple of the
others, some work has been done on staining of cryo-fixed specimens, which
helps with the low contrast of cryo-fixed material. I can't remember the
details of the technique - I guess you actually have to stain the specimen
before cryo-fixation - but I think that a tin compound is used.

Some type of electron spectroscopic imaging would also help with the
contrast, the options are either a bolt-on imaging spectrometer, such as
the one Gatan produce, or the in-coloumn energy filter. Until recently,
Zeiss were the only option here. However, Philips have just launched a
version of their CM120 which has an in-column filter. I'm sure they would
like some nice applications problems for promoting it - I'd give them a
call and see if you can get some free microscope time!

Regards

Larry Stoter






From: colijn.1-at-osu.edu (Henk Colijn)
Date: Mon, 29 Jul 1996 17:43:07 +0400
Subject: Hitachi H9000 NAR available

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millsmj-at-kcgl1.eng.ohio-state.edu, begg-at-kcgl1.eng.ohio-state.edu
Message-id: {v01540b01ae226cefc6a2-at-[164.107.183.168]}
MIME-version: 1.0
Content-type: text/plain; charset="us-ascii"
Content-transfer-encoding: 7BIT

We have a Hitachi H9000NAR TEM that we need to sell. It is a 300kV, 1.8A
resolution, +/-15deg sample tilt high-resolution scope.

Included with the scope are
* single-tilt sample rod,
* double-tilt sample rod,
* single-tilt heating stage,
* turbo-pumped sample rod storage device,
* Gatan 622 video camera,
* Haskris water chiller.

The scope has been continuously under service contract and is in excellent
condition. Asking price is $420,000.

Henk Colijn
colijn.1-at-osu.edu
(614) 292-0674
(614) 292-1537 FAX

Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility
-------------------------------------------------------------------
Murphy's Law: If anything can go wrong, it will.
Commentary: Murphy was an optimist.






From: philf-at-NEWTON.UMSL.EDU (Phil Fraundorf)
Date: Mon, 29 Jul 1996 16:20:53 -0500
Subject: web practice at HREM focusing, astigmation, & CTF modeling

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Know anyone who would enjoy some practice at focusing and astigmating a
high resolution TEM, but doesn't have one at hand? Want to show someone in
your office or classroom what happens to the CTF as a function of defocus?
Our browser-interactive simulator at
{http://www.umsl.edu/~fraundor/epc/index.html} is now up.

You might also contribute to others' learning, by finding Scherzer defocus
or modeling the CTF from images, and then sharing your strategy and results
with others who access the page. You even vote with your feet, since use
patterns as well as your comments will be monitored to help decide what new
"specimens" and/or "scope models" we might host in days ahead.

Cheers. /philf :)

\//
(-at- -at-)
//\/\/\/\--o00-(_)-0oo--}
// P.Fraundorf Phys&Astr/CME 3145165044 philf-at-newton.umsl.edu
\\ U.Missouri-St.Louis MO 63121 http://www.umsl.edu/~fraundor
\\/\/\/\/\/\/\/\/-----------------}





From: Bob Citron :      Bob_Citron-at-cc.chiron.com
Date: 7/29/96 1:46 PM
Subject: ISO or A2LA -- EPMA lab certification

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As part of our organization's efforts to attain ISO9000 certification, I will
soon be faced with the task of preparing our electron microprobe laboratory
for a preliminary audit.

I would like to correspond with anyone who has been throught this experience.


Larry Franks
Franks-at-Inland.com





From: chasrf-at-ichange.com (Charles Fanghella)
Date: Mon, 29 Jul 1996 20:24:14 +0000 (MULTINET_TIMEZONE)
Subject: Unsubscribe

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From: DavidSu-at-aol.com
Date: Tue, 30 Jul 1996 01:34:00 -0400
Subject: Subscribe

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Please add to subscription list

David Su
davidsu-at-aol.com




From: Marc D'Olieslaeger :      mdoliesl-at-luc.ac.be
Date: Tue, 30 Jul 1996 08:55:01 +0200
Subject: (no subject)

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Message-Id: {31FDB1C5.1073-at-luc.ac.be}

unsubscribe
--
dr. Marc D'Olieslaeger
Coordinator Analysis Department
Materials Physics Division
Institute for Materials Research
Limburgs Universitair Centrum
Wetenschapspark 1
3590 Diepenbeek
Belgium
tel. +32-11-26.88.26.
tel +32-11-26.88.15. (direct line)
fax +32-11-26.88.99.
email : mdoliesl-at-luc.ac.be




From: Mat Waldron :      matheww-at-imaging.co.uk
Date: Tue, 30 Jul 1996 11:21:06 -0700
Subject: (no subject)

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From: wiens-at-cobra.uni.edu (Darrell Wiens)
Date: Tue, 30 Jul 1996 08:41:56 -0600 (CST)
Subject: unsubscribe

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From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Tue, 30 Jul 1996 07:52:07 -0700 (PDT)
Subject: Help on Deconvolution

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I am getting confused and nervous (about price) of deconvolution. We are
putting together a deconvolution system for a Nikon SA upright, some type
of cooled CCD monochrome camera (haven't decided which) on a PowerMac
platform.

We already have the microscope and are committed to PowerMac. We have
contacted several companies that can provide the whole system, therefore:

1. I would love to hear from anyone who has had experience in putting such
a system together, with warnings and recommendations.

2. I'm confused about the various terms describing deconvolution:

EPR algorythem
Nearest Nieghbor
Inverse fourier analysis
constrained iterative
Poisson point model
PSF deconvolution
3D blind deconvolution

Is there somone who could simplify this or knows of a good reference that
clarifies the methods?

Bob
University of Washington





From: Harry Murray :      hmurray-at-morgan.ucs.mun.ca
Date: Tue, 30 Jul 1996 11:56:41 -0230 (NDT)
Subject: Insitu and EM

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Help,

I am a grad student working on the development of a technique to local
ize "antifreeze" gene expression in winter flounder gill epithelium. So far,
I have been successful in describing the spatial dynamics of expression using
non-isotopic digoxigen probes and a HRP/DAB marker. My problem lies in
attempts to pin point the specific cell types that are expressing.
In the development of my protocoal, I took whole gill filaments and processed
them for insitu hybridization, getting a nice pattern of expression. My next
step was to embed this tissue in resin and cut semi-thin and ultra-thin
sections which were subsequently scoped to theoretically reveal staining in
association with the involved cells. Unfortunately, I cannot detect any diff
erences between experimentals and controls, infact no staining is
detectable at all. The question is, what is happening to the DAB staining
product and why can't I see it in section? Any ideas??

H. Murray





From: Michael Cammer :      cammer-at-aecom.yu.edu
Date: Tue, 30 Jul 1996 12:38:05 -0400 (EDT)
Subject: Re: Eikonix scanner

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I found that without calibration we had dramatic lines running through
the images. These lines seemed to be from the chip; they were in fixed
locations. The calibration on a blank piece of film and without
saturation solved this problem. Compared to background subtraction, the
images appeared the same.
-Michael Cammer

On Tue, 16 Jul 1996, Philip Koeck wrote:

} Can anybody answer some technical questions about an Eikonix scanner.
}
} Specifically: How does the calibration work?
} With our scanner it doesn't seem to make any difference how You run
} the CALIB program, with lights first on then off or vice versa,
} or on (off) both times.
}
} Does anybody know whom to call for technical support?
} The company in Bedford MA doesn't exist anymore.
}
} Philip
} --
} Philip Koeck
} Karolinska Institutet
} Dept. of Bioscience
} Novum
} S-14157 Huddinge
} Sweden
} Tel.: +46-8-608 91 93
} Fax.: +46-8-608 92 90
} Email: Philip.Koeck-at-csb.ki.se
}




From: Mary A. Molter :      molter-at-post.its.mcw.edu
Date: Tue, 30 Jul 1996 12:45:48 -0500 (CDT)
Subject: vacation

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Need to know proper procedure for holding mail while on vacation.

Thanks in advance.

Mary
molter-at-post.its.mcw.edu





From: Mary A. Molter :      molter-at-post.its.mcw.edu
Date: Tue, 30 Jul 1996 12:35:39 -0500 (CDT)
Subject: suspending mail

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microscopy-at-aaem.amc.anl.gov, IPOX-L-at-patholgy.stanford.edu,
PATHO-L-at-EMUVM1.cc.EMORY.EDU

Need to know proper procedure for suspending my mail while I'm on vacation.

Thanks in advance.

Mary
molter-at-post.its.mcw.edu





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 30 Jul 1996 15:40:12 -0400
Subject: RE-Spec Grid Info

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Message-ID: {n1373380975.7520-at-mse.engin.umich.edu}

Subject: Time: 3:33 PM
OFFICE MEMO RE:Spec Grid Info Date: 7/30/96

You can find brief discussions of the design of TEM specimen grids in:
Techniques for Electron Microscopy, D. H. Kay, Editor. 2nd. Ed,
Blackwell Scientific Pubs. (and F. A. Davis) 1965, Ch. 3,
and in
The Principles & Practice of Electron Microscopy, by Ian M. Watt.
Cambridge Univ. Press 1985, p. 82.







From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 30 Jul 1996 10:48:28 EST
Subject: Re: polysacharide structure and TEM

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microscopy-at-Sparc5.Microscopy.Com

} I have TEM experience only with cells and tissue. Someone has asked me
} if I could show the structure of a pneumococcal polysacharide using TEM.
} I understand that proteins, DNA and viruses are studied using negative
} staining. Does anyone have information about the possibility of seeing
} a sugar molecule for the purpose of comparing two structural forms of it?
} Is there a negative stain? Thanks for any comments.

If the polysacharride is extracellular, ie as a bacterial capsule,
then preparative technique is critical. Bacterial surface capsules,
slime layers, etc. are highly hydrated, and when negative stained and
dried, they condense down into an amorphous mass. This even happens
during dehydration for embedment and sectioning. There are several
methods for stabilizing capsules to quantitatively assess their
distribution, extensiveness, etc. The most straightforward that I
have used uses cationized ferritin to stabilize it prior to fixation
and embedment. Ruthenium red also may be used, but in my experience
is not as satisfactory.
A good starting reference is:

Jacques et al. 1988. J. Bacteriol. 170(7). pp 3314-18.

Good luck.


-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Tue, 30 Jul 1996 10:50:47 -0400
Subject: Stray EM Fields

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Hi All.
I have been having a bit of a problem with some stray EM (?) fields
on our SEM. The problem has been traced to the power feeding the wall
outlets. Anything plugged into these outlets and the microscope will affect
the images produced. Specifically the backscatter detector and the PC we use
to capture images digitally. The results are the same for images captured
photographically if either the backscatter or PC is plugged into the wall.
They are not the cause of the problem but simply feed the noise to the
microscope via any cables connected to it. If I unplug all from the outlets
or unplug cables leading to the microscope the problem is corrected. It
doesn't matter if they are on or not, it still feeds the noise.

We have so far simply run extension cords to other outlets in other
rooms and on other circuits in order to solve this problem but it has
worsened and I am running out of different outlets. I recently purchased an
APC line conditioner which is supposed to continuously condition the power
and give out a fresh signal but needless to say it hasn't worked or I
wouldn't be asking for your help.

If you would like to see an image with the problem go to the web
address at the bottom of this message and look in "What's New". You will
notice the vertical banding pattern produced.

Your help and expertise will be greatly appreciated. Vendors please
jump in with any advise you may have.


Sincerely




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "





From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Tue, 30 Jul 1996 13:27:01 -0700 (PDT)
Subject: Re: Insitu and EM

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On Tue, 30 Jul 1996, Harry Murray wrote:

}
} Help,
}
} I am a grad student working on the development of a technique to local
} ize "antifreeze" gene expression in winter flounder gill epithelium. So far,
} I have been successful in describing the spatial dynamics of expression using
} non-isotopic digoxigen probes and a HRP/DAB marker. My problem lies in
} attempts to pin point the specific cell types that are expressing.
} In the development of my protocoal, I took whole gill filaments and processed
} them for insitu hybridization, getting a nice pattern of expression. My next
} step was to embed this tissue in resin and cut semi-thin and ultra-thin
} sections which were subsequently scoped to theoretically reveal staining in
} association with the involved cells. Unfortunately, I cannot detect any diff
} erences between experimentals and controls, infact no staining is
} detectable at all. The question is, what is happening to the DAB staining
} product and why can't I see it in section? Any ideas??
}
} H. Murray
}
}
Is the tissue osmicated? Are the sections counterstained? If so the DAB
may be lost visually. You may try a thicker section and veiw them with a
lower accellerating voltage at the scope in order to increase the contrast
between any electron dense DAB and tissue. Without counterstaining.

Bob, Morphology Core





From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 30 Jul 1996 11:12:02 EST
Subject: Re: duodenal brush border vesicles

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} Duodenal brush border vesicles - DBBV- and negative staining.
} We do negative staining of duodenal content of ostriches regularly as
} well as faeces samples of dogs, calves, etc to detect the
} presence of any possible virus particles eg. parvo, corona, picorna,
} rota, etc. However we experience some difficulties in diagnosing
} paramyxovirus (orthomyxo?) particles beyond doubt. The problem arises
} when we encounter paramyxovirus-like particles from low numbers to
} masses of them in a specimen BUT no sign of any helical nucleoprotein
} strands. How can we break up these particles to release any
} nucleocapsid strands if they are virus particles?

These "virus-like" particles that you describe are a source of much
debate among those of us who look at negative-stained fecal preps. I
find them routinely in ostriches, emus, and turkeys, both ill and
clinically healthy birds. If you suspect infection with a
pleomorphic, enveloped virus, such as coronavirus or paramyxovirus,
these things really are a nuisance. I don't believe that there is
any known way of getting rid of them, short of lysis in distilled
water. This is not completely effective, as most of them are already
in lysis to some extent.

} Can anyone supply me with a micrograph of x100-150K magnification of
} DBBV (or any other reference) to compare with our own known
} micrographs of paramyxoviruses or give info on how to obtain DBBV
} easily(?) for negative staining.

See Goodwin et al. 1995. Avian Pathology 24(3) pp 497-505.

} Q: What happens to the duodenal epithelial lining during a viral
} (bacterial) infection; can it shred structures (vesicles) in such vast
} numbers and do they have a viral-like appearance with negative
} staining?

Gut is constantly turning over enterocytes, with the old ones being
shed as "effete" enterocytes. These are basically membrane ghosts,
which explains why you often see them in both healthy and ill
animals. The glycocalyx on the membrane surface when negative
stained often has a fuzzy or spiked appearance, and is very easily
confused with enveloped viruses. Their wide range in size and
surface projection morphology is what I use to distinguish them from
real viruses.



-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Jill Craig :      jcraig-at-unbc.edu
Date: Tue, 30 Jul 1996 15:50:22 -0700 (PDT)
Subject: Denton Vacuum Critical Pt. Dryer

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Hi,

I have a new Critical Pt. Dryer from Denton Vacuum. I have not used a
critical point dryer before and the configuration of this one is
confusing. I would appreciate hearing from anyone with a similar model
who would be willing to talk me through a first run.

Thanks, Jill




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: 30 Jul 96
Subject: MSA Meeting/Minneapolis

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Message-Id: {199607301818.OAB09062-at-mime2.prodigy.com}
X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc04

-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #3.0 ] --

Special notice to those on the listserver, domestic and foreign,
attending the MSA meeting in Minneapolis, August 12-16:

As a special courtesy to those who have been participating on the
Microscopy listserver, SPI Supplies will, once again, offer
complimentary access to its telephone and FAX machine that will be set
up in the SPI Supplies exhibit booth at the MSA meeting. Just bring
your FAXes and say you are from the "Microscopy Listserver" and the
booth staff will know that they should just send your FAX. That is all
there is to it.

SPI makes this offer in the spirit of promoting eye-ball to eye-ball
contact with those who have been communicating via the listserver.

Chuck

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From: kszaruba-at-MMM.COM
Date: Tue, 30 Jul 1996 17:35:35 -0500
Subject: Re: LR White embedding

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hans-martin.vaihinger-at-rz.ruhr-uni-bochum.de wrote:
}
} Dear Microscopists
}
} We are only just beginning to use LR White as embedding medium for
} use in EM- immunocytochemistry. A manual by AGAR SCIENTIFIC LTD.
} distributed through PLANO recommends to bring the specimen from
} 70 % EtOH into LR White/70 % EtOH (2:1) before embedding in pure LR
} White. We find that this mixture very quickly separates into two
} phases. How shall we handle this?
} Furthermore the specimen seems to be cured insuffiently even after
} 24 hours of polymerisation at 55 C. Any comments on this?
}


I am having exactly the same experience with LR White and would appreciate
seeing the replies. (I have started going to 95% ethanol in order to get
good mixing).

Thanks!

Karen Zaruba E-mail: kszaruba-at-mmm.com
3M Company Phone: (612) 737-2971
St.Paul, MN USA




From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 30 Jul 1996 16:16:56 -0400
Subject: RE-Vac Mail

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Message-ID: {n1373379015.24948-at-mse.engin.umich.edu}

Subject: Time: 3:55 PM
OFFICE MEMO RE:Vac Mail Date: 7/30/96

Mary:
I have faced the problem you refer to rather frequently when I have to
be out of town on consulting trips and vacation. To a large extent what you
do will depend on the situation for your local computing system, which is the
one that has to handle incoming mail while you are away. If the system has
the capacity to do so, you can simply let the messages accumulate in your
local receiver system, and then go over them when you get back. I have done
this when I have been away for as much as a month, and I have only gotten
warning messages saying I had exceeded the allowed number of stored messages,
but our system is large enough so that nothing ever seemed to get discarded.
If your system has limited capacity it will probably become overloaded and
start discarding incoming messages (but then you'll lose them anyway if you
follow the suggestion in the next paragraph). I suggest you check with your
local system operator on this matter. There may be some optimum protocol you
can follow to minimize stress all around.

If you don't like this kind of an approach you can unsubscribe from the
listserver before you leave, and then subscribe again when you get back.
Basicaly, however, the problem is not one of concern to the listserver, but
one involving what goes on at the receiving end of the listserver operation
in your own institution.

Have a good vacation, WCB





From: garyc-at-stud.unit.no (Gary)
Date: Tue, 30 Jul 1996 22:00:46 +0200 (MET DST)
Subject: Re: TEM - grids/specimen supports

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On Thu, 25 Jul 1996 generalmicro-at-CCINET.AB.CA wrote:

} Date: Thu, 25 Jul 1996 03:44:25 -0600
} From: generalmicro-at-CCINET.AB.CA
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: TEM - grids/specimen supports
}
}
} Hello,
}
} I wish to understand the design and usage of the various grids available
} for TEM today, and so I would like to ask if anyone could direct me to
} references in the literature that pertain to the grids themselves, and the
} methods of preparing them for a specimen, whether the references be for
} common or specialized specimen supports, and in any area of TEM
} application.
}
} I want to know things like: why they are the way they are (some aspects are
} intuitively understandable, but perhaps there are some interesting
} subtleties in the reasons); how they are typically used in practice; how
} they perform in the TEM (such as how they affect the results); and what the
} ideal specimen support in any given application might be. If anyone has
} information related to these questions that is not embodied in a published
} source, I would be happy to learn of it, too.
}
} Cheers,
}
} Cam
}
} ____________________________________________________________________________
} Box 1932 Main Station T: 1 403 435 2167
} Cameron Sorlie, President Edmonton, AB T5J 2P3 Canada F: 1 403 433 9376
} General Microdevices, Inc. -----------------------------------------------
} generalmicro-at-ccinet.ab.ca
} ______________________________________________________
} Microtechnology products for science and industry
}
Cam,
}

You can read this article that have helped me in prepearing the grids for TEM:

Fahrenbach, W.H., Continuous Serial Thin Sectioning for Electron
Microscopy, J. of electron Microscopy ,Technique 1:387-398 (1984).



/////////////////////////////////////////////////////////////////
// // //

// Gary Chinga // email :garyc-at-james.avh.unit.no //
// Plantebiosenteret // WWW :http://www.nvg.unit.no/~gary //
// NTNU, 7055 Dragvoll // phone : 73590168 //
// Norway // fax : 73590177 //
// // //
/////////////////////////////////////////////////////////////////






From: kszaruba-at-MMM.COM
Date: Tue, 30 Jul 1996 17:54:05 -0500
Subject: Re: Data Management

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smithp-at-agresearch.cri.nz wrote:
}
} We have used microsoft access for a number of years and have found it to be
} excellent for data management, storage of block information, etc etc.Its
} compatibility with other software packages also makes it useful, its worth
} having a look at.
}


Regarding Microsoft Access, how does it handle large text fields? And most
importantly, can it search for any part of the text in a field? (Example:
in a search for "skin" would it bring up a field containing "porcine ligament
and skin samples"?)

A couple years ago I spent quite a bit of time trying to set up a database of
all the information that we keep in our "Histopathology" logbook, including
specimen ID, description, experimental/surgical parameters, contact names,
project name, etc. etc. The hope was that this would include all samples for
LM, TEM or SEM and would not only be useful to us in tracking samples and
generating reports, but also be accessible by our managers/primary
investigators to check on progress and results.

Needless to say this was a bit of a pipe dream. At the time I needed PC/Mac
cross-platform compatibility and the only "easy" choice was Filemaker Pro by
Claris. This software was extremely easy to use and could have worked well
for the initial stage (reproducing the logbook). However, it was limited in
ability to "share" fields and their information from one file to another.
Eventually these software limitations and the difficulty of cross-platform
compatibility forced us to cancel the whole endeavor. :(

However, if Access or some other software would be capable of handling
searches of large text fields then I would be interested to hear of it!

Karen Zaruba E-mail: kszaruba-at-mmm.com
3M Company Phone: 612-737-2971
St. Paul, MN USA

Disclaimer: These opinions are my own and may not represent those of 3M.




From: kris-at-almos.vein.hu (Kris Kovacs)
Date: Wed, 31 Jul 1996 08:30:41 +0200
Subject: Stray EM Fields?

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To Scott Whittaker:

I am almost absolutely certain that your problem is associated with an
earthing (or grounding) loop. It is very important to have only one single
earth path. If you have separate earth path as supposedly exists with your
additional backscatter detector and/or PC (the microscope is connected to
the earth terminal, the PC is connected too, and both instruments are
connected to each other through their grounds), there are alternative,
physically separated earth paths, which will result in noise currents, and
almost certainly impair the performance of any electron microscope. Some
years ago we had similar problems with our computer and EDS system, but
there is a remedy. It would be too long and sketches are also needed to
explain. The best I can propose is to check a good textbook such as "Design
of the Electron Microscope Laboratory" by Ronald H. Anderson (North Holland,
1985, p61-66) or consult an expert electrician as a final solution. In case
you cannot obtain quickly the book I can fax you these pages.

Good luck!

Kris

Kristof KOVACS
Associate Professor
University of Veszprem, Central Laboratory
P.O.Box 158, Veszprem, HUNGARY
H-8201
Phone: +36-(88)-421-684





From: Mike Gregory :      mgregory-at-pixie.udw.ac.za
Date: Wed, 31 Jul 1996 09:08:59 +0200 (SST)
Subject: Re: duodenal brush border vesicles

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Hi Buddy:

Sounds like you're describing structures that I found associated with the
glycocalyx of enterocytes during duodenal ulceration. They have been
called glycocalyceal bodies or R-bodies. No one seems to know what they
do or precicely where they come from. A good review article on glyc.
bodies is

P.B.Marcus "Glycocalyceal bodies and their role in tumour typing" J.
Submicroscop. Cytol: 1981: 13: (3) 483-500.

Incidently, if anyone out there has any more recent references or knows
what glyc. bodies are or where they come from I'd be interested in the
information

Regards

Mike Gregory

On Tue, 30 Jul 1996, Buddy Steffens wrote:

} } Duodenal brush border vesicles - DBBV- and negative staining.
} } We do negative staining of duodenal content of ostriches regularly as
} } well as faeces samples of dogs, calves, etc to detect the
} } presence of any possible virus particles eg. parvo, corona, picorna,
} } rota, etc. However we experience some difficulties in diagnosing
} } paramyxovirus (orthomyxo?) particles beyond doubt. The problem arises
} } when we encounter paramyxovirus-like particles from low numbers to
} } masses of them in a specimen BUT no sign of any helical nucleoprotein
} } strands. How can we break up these particles to release any
} } nucleocapsid strands if they are virus particles?
}
} These "virus-like" particles that you describe are a source of much
} debate among those of us who look at negative-stained fecal preps. I
} find them routinely in ostriches, emus, and turkeys, both ill and
} clinically healthy birds. If you suspect infection with a
} pleomorphic, enveloped virus, such as coronavirus or paramyxovirus,
} these things really are a nuisance. I don't believe that there is
} any known way of getting rid of them, short of lysis in distilled
} water. This is not completely effective, as most of them are already
} in lysis to some extent.
}
} } Can anyone supply me with a micrograph of x100-150K magnification of
} } DBBV (or any other reference) to compare with our own known
} } micrographs of paramyxoviruses or give info on how to obtain DBBV
} } easily(?) for negative staining.
}
} See Goodwin et al. 1995. Avian Pathology 24(3) pp 497-505.
}
} } Q: What happens to the duodenal epithelial lining during a viral
} } (bacterial) infection; can it shred structures (vesicles) in such vast
} } numbers and do they have a viral-like appearance with negative
} } staining?
}
} Gut is constantly turning over enterocytes, with the old ones being
} shed as "effete" enterocytes. These are basically membrane ghosts,
} which explains why you often see them in both healthy and ill
} animals. The glycocalyx on the membrane surface when negative
} stained often has a fuzzy or spiked appearance, and is very easily
} confused with enveloped viruses. Their wide range in size and
} surface projection morphology is what I use to distinguish them from
} real viruses.
}
}
}
} -=W.L. Steffens=-
} Department of Veterinary Pathology
} College of Veterinary Medicine
} University of Georgia
}




From: Interface Analysis Centre :      K.R.Hallam-at-bristol.ac.uk
Date: Wed, 31 Jul 1996 10:30:32 BST
Subject: Re: RE-Vac Mail

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} If you don't like this kind of an approach you can unsubscribe from the
} listserver before you leave, and then subscribe again when you get back.
} Basicaly, however, the problem is not one of concern to the listserver, but
} one involving what goes on at the receiving end of the listserver operation
} in your own institution.
}
In case you are on any other lists, be aware that some listowners unsubscribe
people if too many messages get bounced back once the receivers mailbox is full
(happens to one list I am on every so often and usually takes me a month to notice
:-)

Keith
---
Interface Analysis Centre, University of Bristol, Oldbury House,
121, St. Michael's Hill, Bristol, BS2 8BS, England
Telephone: +44 (0)117 925 5666 | Facsimile: +44 (0)117 925 5646 |
URL: http://www.phy.bris.ac.uk/research/iac/home.html






From: John Best :      jbest-at-vicon.net
Date: Wed, 31 Jul 1996 08:31:36 -0700
Subject: Re: Stray EM Fields

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Message-Id: {9607311115.AA00401-at-easynet.crl.dec.com}

Scott,

This is an odd image. Typically, with periodic EM or mechanical
interference, you'll see jagged edges. The amplitude of the "jagginess"
increases proportionally with magnification.

I assume in this image your referring to the banding on the substrate. Is
this correct?

My best guess at this time if that the scan rotation was at 90 degrees
and you've left the SEM's ABC circuit on during image collection.

John Best -- ELMDAS Co.
jbest-at-vicon.net





From: Walter A. Mannheimer :      wamann-at-metalmat.ufrj.br
Date: Wed, 31 Jul 1996 09:56:01 EST3EDT
Subject: IRFT microscopy

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I would like to contact microscopist operating IRFTmicrocopy
(reflected light). My interest in in ageing of polymeric (electrical
insulation) materials.
Perhaps anyone would care to meet at the MSA meeting in Minneapolis
(notice on the bulletin board?)
Thank you
Prof.Walter A.Mannheimer
Metallurgy and Materials Engineering
Federal University of Rio de Janeiro
POBox 68505 21945 Rio de Janeiro, Brazil
Voice +5521 280-7443 (Dept.office) +5521 590-0579 (direct)
Fax +5521 290-6626 Email: wamann-at-metalmat.ufrj.br




From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Wed, 31 Jul 1996 13:48:03 +0000
Subject: Re: Stray EM Fields

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X-Sender: (Unverified)
Message-Id: {v01510100ae251243455a-at-[158.152.199.245]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Hi All.
} I have been having a bit of a problem with some stray EM (?) fields
} on our SEM. The problem has been traced to the power feeding the wall
} outlets. Anything plugged into these outlets and the microscope will affect
} the images produced. Specifically the backscatter detector and the PC we use

snips...

} Your help and expertise will be greatly appreciated. Vendors please
} jump in with any advise you may have.

Scott,

You are almost certainly setting up ground loops. ALL your equipment must
go back to a common, local ground. You need to go back to the mains power
source for your SEM and run extensions to all auxillary equipment from this
point. Be careful not to make these extensions too long, or you may suffer
pick-up from other sources. There should be some auxillary power outputs in
the back of your SEM and these are the prefered power source. However, if
you have a lot of auxillary equipment, you may risk overloading these.

Ideally, you should have a mains matching trasnformer between your SEM and
your local power supply - this helps to provide protection agianst power
surges, and some isolation from interference. You should tap in at this
point to provide additional power sources.

Regards,

Larry Stoter






From: John Best :      jbest-at-vicon.net
Date: Wed, 31 Jul 1996 08:57:39 -0700
Subject: Re: Stray EM Fields?

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Message-ID: {31FF8273.2E50-at-vicon.net}

To Scott Whittaker:
Normally I'd agree with Kris Kovacs assesmmment, but usually noise from
ground loops is much more periodic, as the dominant ground currents are
usually 60 Hz.

A proper analysis of the instrumentation should be performed, but this is
often impractical. Often floating the 110VAC inputs to the PC and BSE
(using "cheater plugs") and grounding their frames directly to the
instrumentation ground of the SEM (via very low impedance braided cables)
will solve this problem. However, this approach is more of a diagnostic
procedure than a permanent remedy. Extreme care must be taken if this
solution is employed, as uninsulated ground cables running about in your
SEM can cause havoc if they come in contact with the wrong thing. Also,
although this may be a better instrumentation practice, it isn't the best
for safety reasons, as your depending on the ground cables (and the SEM
earth ground) for safety grounding. If you choose to employ this
approach, DISCUSS IT WITH YOUR LOCAL MAINTENANCE PEOPLE. I assume no
responsibility for the situation.


Kris Kovacs Wrote:
} I am almost absolutely certain that your problem is associated with anearthing (or grounding) loop. It is very important to have only one
single earth path. If you have separate earth path as supposedly exists
with your additional backscatter detector and/or PC (the microscope is
connected to the earth terminal, the PC is connected too, and both
instruments are connected to each other through their grounds), there are
alternative, physically separated earth paths, which will result in noise
currents, and almost certainly impair the performance of any electron
microscope. Some years ago we had similar problems with our computer and
EDS system, but there is a remedy. It would be too long and sketches are
also needed to explain. The best I can propose is to check a good
textbook such as "Design of the Electron Microscope Laboratory" by Ronald
H. Anderson (North Holland, 1985, p61-66) or consult an expert elctrician
as a final solution. In case you cannot obtain quickly the book I can fax
you these pages.
} End of Kris Kovacs reply





From: kna101-at-utdallas.edu
Date: Wed, 31 Jul 1996 10:13:44 -0500 (CDT)
Subject: Re: Stray EM Fields?

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From: luciom-at-NEWTON.UMSL.EDU (Luciano Mule'Stagno)
Date: Wed, 31 Jul 1996 09:00:43 -0500
Subject: St. Louisans going to MSA

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Message-Id: {9607311355.AA08769-at-aurora.memc.com}
X-Sender: luciom-at-newton.umsl.edu (Unverified)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hi there,
I am trying to locate someone from St. Louis going to MSA. I have
submitted an abstract, but will not be able to make it to Minneapolis on
monday. I am trying to find someone to hang it up for me.

thanks

Lucio

ps - please reply to me directly at one of the emails below.


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Lucio Mule'Stagno
MEMC Electronic Materials Inc University of Missouri -St.Louis
Silicon Materials Research Group Physics Dept.,
501 Pearl Dr., 8001 Natural Bridge rd.,
St.Peters, St.Louis
MO 63376 MO 63121
tel: 314 279 5338 314 516 5931/3
fax: 314 279 5363 314 516 6152
email: lmulestagno-at-memc.com luciom-at-newton.umsl.edu
URL:http://www.newton.umsl.edu
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From: ayache-at-csnsm.in2p3.fr (Ayache Jeanne)
Date: Wed, 31 Jul 1996 16:00:07 +0000
Subject: suspend mail during vacations

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Message-Id: {199607311401.QAA23843-at-csn-hp.in2p3.fr}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"



stop sending mail during all august month long please

Thank you

Jeanne Ayache
CSNSM-CNRS
Bat 108
91405 Orsay campus
France
ayache-at-csn-hp.in2p3.fr (Ayache Jeanne)








From: kna101-at-utdallas.edu
Date: Wed, 31 Jul 1996 10:26:56 -0500 (CDT)
Subject: Cell coats

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Dear Pat,

I'm not sure if this is what your looking for, but alcian blue 8GX can be
added to glutaraldehyde fixative to enhance preservation and contrast of
cell coats and, if part of the coat contains glycosaminoglycans, alcian
blue is
better at maintaining the thickness of the coat than ruthenium red.
Electron Microscopy Sciences has it (Ft. Washington, PA), we have an old
jar of it from Sigma Chem Co (St. Louis, MO) and I'm sure there are other
suppliers. The reference EMS gives on fixation is Schofield, et al.,
Histochem J. 7:139 (1975).

Good luck,
Karen




From: mrdunlap-at-ucdavis.edu (Michael Dunlap)
Date: Wed, 31 Jul 1996 11:03:54 -0700
Subject: McKenzie Buffer Recipe

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Hello fello micropist -

We are looking for a buffer for snake eyes. Not snake oil. Someone
sugested a Mckenzie Buffer. If anyone has a recipe for this or an
additional buffer for snakes we would like to know.

thanks

Mike

===========================================================
Michael Dunlap lab (916) 752-0284
Facility For Advanced Instrumentation fax (510) 422-2282
University of California mrdunlap-at-ucdavis.edu
Davis CA, 95616
http://carbon.ucdavis.edu
============================================================






From: wesleysm-at-biology.und.ac.za (James Wesley-Smith)
Date: Wed, 31 Jul 1996 17:34:44 +0200
Subject: Yet another LR White query!

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Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za}

I would like to add another 'complaint' to the list. Plant tissues
embedded in LR White show signs of severe plasmolysis. I have broken
down the infiltration into 25, 50, 75 and 100% resin/75% EtOH and
prolonged the infiltration stages (+- 2 hrs each) but to
no avail.

By the way, the mixture tends to separate as well! Maybe this is the
reason for the shrinkage...?

###################################################
James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa
http://www.und.ac.za/und/emu/emunit.html
###################################################




From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Wed, 31 Jul 1996 12:08:53 -0600
Subject: TEM/LM flat bottom "beem" caps

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Message-Id: {v01540b09ae2550429b92-at-[128.206.15.200]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Greetings,
We have been using some capsules that are like "beem" capsules,
8mm diameter and with a snap-on cap, only instead of having a cone or a
pyramid, they are simply flat on the bottom. They are made from
polyethylene (polypropylene ones are made too). These are great for flat
embedding. We got ours from TAAB in England, but we have used them up
nearly. Does anyone know if these are for sale in the USA? A quick perusal
of catalogs from several major USA supply houses failed to find 'em. Thanks
for any leads.
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: Delilah W. Irving :      dirving-at-aggie.pw.usda.gov
Date: Wed, 31 Jul 1996 06:47:21 -0700 (PDT)
Subject: Re: LR White embedding

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I've used (or rather TRIED to use)LR White for LM but with very little
success. I found that the blocks were brittle and didn't stain well with
LM stains. Needless to say, I'm not impressed and have stuck to GMA
embedding. I understand that the shelf-life for LR White is very short;
perhaps that was part of my problem as well as part of yours???

On Tue, 30 Jul 1996 kszaruba-at-mmm.com wrote:

} hans-martin.vaihinger-at-rz.ruhr-uni-bochum.de wrote:
} }
} } Dear Microscopists
} }
} } We are only just beginning to use LR White as embedding medium for
} } use in EM- immunocytochemistry. A manual by AGAR SCIENTIFIC LTD.
} } distributed through PLANO recommends to bring the specimen from
} } 70 % EtOH into LR White/70 % EtOH (2:1) before embedding in pure LR
} } White. We find that this mixture very quickly separates into two
} } phases. How shall we handle this?
} } Furthermore the specimen seems to be cured insuffiently even after
} } 24 hours of polymerisation at 55 C. Any comments on this?
} }
}
}
} I am having exactly the same experience with LR White and would appreciate
} seeing the replies. (I have started going to 95% ethanol in order to get
} good mixing).
}
} Thanks!
}
} Karen Zaruba E-mail: kszaruba-at-mmm.com
} 3M Company Phone: (612) 737-2971
} St.Paul, MN USA
}

Delilah W. Irving tel: 510-559-5653
USDA - ARS - WRRC fax: 510-559-5777
800 Buchanan St. email: dirving-at-pw.usda.gov
Albany, CA 94710






From: Brian Gorman :      bgorman-at-umr.edu
Date: Wed, 31 Jul 1996 16:15:42 -0500 (CDT)
Subject: Re: LR White embedding

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Message-Id: {199607312115.QAA05848-at-rocket.cc.umr.edu}
To: microscopy-at-Sparc5.Microscopy.Com

subscribe microscopy bgorman-at-umr.edu




From: Doug Cromey :      dcromey-at-ccit.arizona.edu
Date: Wed, 31 Jul 1996 07:59:16 -0700
Subject: vacations from the list

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Listmembers,
I don't know if this works on every list, but a technique I've used to stop
emailbox overflow when I'm out of town is to use the following command:

the TO: address should be LISTSERV-at-MSA.MICROSCOPY.COM
in the text of the message put the line (no signature or footers):
SET MICROSOPY NOMAIL

When you're ready to get your mail again, send the following message to the
listserv address (LISTSERV-at-MSA.MICROSCOPY.COM):
SET MICROSCOPY MAIL

It's probably about as effective as unsubscribing and resubscribing
(provided you remember how to do that without sending your request to all of
us list readers). It does have the advantage of avoiding the welcome
messages that come when you resubscribe (not that we mind Nestor's eloquent
prose...).

Have a safe vacation.

Doug
.....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
:...................................................................:
http://www.pharm.arizona.edu/exp_path.html





From: kevin-at-mediacy.com
Date: Wed, 31 Jul 1996 14:00:19 -0400
Subject: TEM Job Opening at Intel Albuquerque

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} From: Robert Underwood {underwoo-at-u.washington.edu}
} To: Microscopy List {Microscopy-at-Sparc5.Microscopy.Com}
} Subject: Help on Deconvolution
} Date: Tuesday, July 30, 1996 10:52 AM
}
} I am getting confused and nervous (about price) of deconvolution. We
are
} putting together a deconvolution system for a Nikon SA upright, some
type
} of cooled CCD monochrome camera (haven't decided which) on a PowerMac
} platform.
}
} We already have the microscope and are committed to PowerMac. We have
} contacted several companies that can provide the whole system,
} therefore:
}
} 1. I would love to hear from anyone who has had experience in putting
such
} a system together, with warnings and recommendations.
}
} 2. I'm confused about the various terms describing deconvolution:
}
} EPR algorythem
} Nearest Nieghbor
} Inverse fourier analysis
} constrained iterative
} Poisson point model
} PSF deconvolution
} 3D blind deconvolution
}
} Is there somone who could simplify this or knows of a good reference
} that clarifies the methods?
}
} Bob
} University of Washington

I've written deconvolution software in the past (although I'm not
working with it right now), and perhaps I can answer a few things...


The Point Spread Function (Optical Transfer Function, Modulation
Transfer Function, all different forms of the same thing) describes how a
'point object', smaller than the resolution of the scope, blurs in 3-D.
As large objects can be considered as clouds of little points, this gives
all the information about how any complex object gets blurred.

This blur is reversed to sharpen the data, providing a reconstruction
of what the original object was. The relationship is:

D = P*O; P(-1)*D = P(-1)*P*O ~ O

The data is the object convolved with the point spread function,
reconstruction consists of convolving the data with the inverse point
spread function to approximate the object. This is approximate, as some
parts of the PSF are zeros, and you lose that information permanently. In
addition, 1/0 is infinity, and tends to be badly behaved mathematically.

Fourier transforms are used to simply these calculations - a
multiplication in the Fourier domain is equivalent to convolution in a
normal image.

Full inverse deconvolution involves taking the 3-D Fourier of the PSF
and data, inverting the PSF (with math tricks to handle zeros),
multiplying it times the data and inverse Fourier transforming the
result. Lots of number crunching.

Constrained iterative runs multiple cycles of this, 'constraining'
the result at each pass with limits such as non-negativity or frequency
limits (things you _know_ about the object), to minimize the errors from
the lost data and contaminating noise. Lots of number crunching, repeated
25-50 times, rather better results than a single pass.

Nearest Neighbor is an approximation of full single inverse
deconvolution (fairly good, actually), that uses the data planes above
and below each plane to estimate out of focus blur, and output an object
approximation. Less accurate, but a _lot_ less computation. This is also
more appropriate for _thin_ specimens, as you really need a fair number
of planes for decent full deconvolution. Full iterative deconvolution
surpasses nearest neighbor accuracy at about iteration 10 to 15 out of
50.

EPR I'm not so certain of - that sounds like one of the maximum
entropy techniques. If it is, it has computation on the order of
constrained iterative, with some mathematical pluses and minuses to
differentiate it.

Poisson point model; that term sounds like a noise model. When
handling places where the PSF approaches zero you have to have a good
model of the data SNR (signal to noise ratio) to see how close you can
cut matters. Poisson, Gaussian, and white noise are all different models
that have been used, depending on acquisition methods, experimental
knowledge, and the bias of the experimentor.

3-D blind deconvolution refers to techniques of estimating the PSF in
the absence of exact knowledge. I have not used such algorithms, and I
can't tell you how good they are. In general, if you can get a good
estimate of the PSF (using analytic techniques if you have low
abberations or experimental PSF's if not) you will probably have a better
reconstruction than if your program guesses at it from the
characteristics of your data.


Warnings and recomendations:

Match your immersion fluid to your embedding media in regards to
refractive index. Otherwise changing relative path lengths at different
depths and refractive indexes in your specimen result in depth dependent
spherical abberations, meaning that your PSF is highly non-uniform and
non-linear, and your reconstruction suffers accordingly. The best results
I've seen are from water immersion and no cover slip, the second best
from water or glycerin immersion and wet mounts with cover slips. Good
objectives are equally important, particularly in regards to Plan (flat
field of view) qualities.

Consider time: iterative techniques are ones where you go away for
lunch or the evening, and look at results the next day. Nearest neighbor
can be done in a few minutes at most. Confocal scopes give you immediate
results (at the cost of massive photobleaching and longer scan times).

Get good data, with a high SNR. I once had someone come in with some
data that had a dynamic range of 35 grey values on 20 grey values of
noise (SNR of ~1.5), and ask for a reconstruction. Results were, shall we
say, poor. Since you're amplifying edges, salt and pepper noise will have
a major effect compared with the smooth blurred object edges. Starting
with a high signal to noise ration mitigates this.

Correct for non-linearities in your imaging prior to deconvolution!!!
This means background subtraction, flat field division, and scaling the
images in your stack to account for photobleaching, in that order. [For
bleaching, you can just assume that all full field images of the same
area, regardless of depth, will have the same integrated intensity, and
scale accordingly.] The PSF division technique is based on assumptions of
linear modulations in the object, and non-linear effects such as these
_will_ distort your results.

Cost: not much that can be said here. It will likely cost you on the
order of a low to medium grade confocal microscope to implement
deconvolution.

Most importantly - data acquisition is really only a minor piece of
the bag. Deconvolution in its various forms, confocal, whatever; you'll
get 3-D data. Data analysis, rendering and the extremely difficult 3-D
measurements of position, area, and volume: these are what will pay off
in the long run. I won't make product recommendations, but I _strongly_
suggest that you consider your analysis software (integrated or separate)
as a major item in your system design. Review these with as critical an
eye as the acquisition method. Great data only pays off if you can derive
answers from it.

Hope this is helpful!

--
Kevin Ryan
Media Cybernetics
kevin-at-mediacy.com




From: kszaruba-at-mmm.com
Date: Tue, 30 Jul 1996 17:35:35 -0500
Subject: Re: LR White embedding

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Message-ID: {n1373297827.69710-at-sjdccd.cc.ca.us}

We have used LR White for over 15 years. The biggest problem that we have is
when there is EtOH left in the material before it goes to 100% LRWhite. To
take care of that problem we do NOT put a mixture of alcohol and LR Whitae
before going to pure. We definitely do go to 100%, then 3 changes of pure LR
White, then into capsules. The gelatinous mess that results from the EtOH/LR
White was never found to polymerize even after 2 yrs., even after try ing to
dry it out and all sorts of other manipulations to save a valuable sample.
Good Luck,
Judy Murphy


Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy-at-ms.sjdccd.cc.ca.us

_______________________________________________________________________________

hans-martin.vaihinger-at-rz.ruhr-uni-bochum.de wrote:
}
} Dear Microscopists
}
} We are only just beginning to use LR White as embedding medium for
} use in EM- immunocytochemistry. A manual by AGAR SCIENTIFIC LTD.
} distributed through PLANO recommends to bring the specimen from
} 70 % EtOH into LR White/70 % EtOH (2:1) before embedding in pure LR
} White. We find that this mixture very quickly separates into two
} phases. How shall we handle this?
} Furthermore the specimen seems to be cured insuffiently even after
} 24 hours of polymerisation at 55 C. Any comments on this?
}


I am having exactly the same experience with LR White and would appreciate
seeing the replies. (I have started going to 95% ethanol in order to get
good mixing).

Thanks!

Karen Zaruba E-mail: kszaruba-at-mmm.com
3M Company Phone: (612) 737-2971
St.Paul, MN USA

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From: IAN HALLETT :      ihallett-at-MARCCRI.MARC.CRI.NZ
Date: Thu, 1 Aug 1996 12:31:02 GMT+1200
Subject: Re: LR White embedding

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We have used LR White routinely for a number of years. We find the
best results are obtained by dehydrating to 100% Ethanol. The main
things to watch out for are keeping the temperature well controlled
and excluding oxygen (which can penetrate many commonly used moulds
such as BEEM capsules).

Excessive brittleness seems to be caused by the embedding
temperature being too high (perhaps only a degree or two above 60C).

Fresh LR White has a shelf life of 1 year at 4C though we have used
older batches occasionally. The major problem with old batches can
be premature polymerising. This is not a great problem if it occurs
in the bottle but more so if it occurs whilst infiltrating the
sample. Uncatalysed resin, which comes with a powder catalyst,
has an almost indefinite shelf life and we make up a fresh bottle of
active resin just before the old one runs out.

Ian



Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660
EMail ihallett-at-hort.cri.nz




From: John Best :      jbest-at-vicon.net
Date: Wed, 31 Jul 1996 21:17:44 -0700
Subject: Re: Stray EM Fields

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Message-ID: {32002FE8.1656-at-vicon.net}

Robert and all,

In my experience, the "high mag jaggies" are most often caused by a
mechanical vibration. Often the roughing pump is the problem. Sometimes
a couple of tennis balls under the roughing pump will decrease the effect
substantially. It's not always this simple though. Also check for cables
touching the vacuum line from the RP. They can transmit these vibes.

John






From: KEN STANLEY :      k.stanley-at-student.qut.edu.au
Date: Thu, 01 Aug 1996 11:30:44 +1000 (EST)
Subject: TEM - Detection of bacterial infection in cultured lymphocytes.

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I need to find out how I could determine if a suspected bacterial
infection of cultured lymphocytes is in fact the case.

Any assistance, advice or direction of where to find infomation would
be greatly appreciated.
Thankyou
Ken Stanley
k.stanley-at-student.qut.edu.au






From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Thu, 1 Aug 1996 10:39:39 +1100
Subject: Re: LR White embedding

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} Dear Microscopists
}
} We are only just beginning to use LR White as embedding medium for
} use in EM- immunocytochemistry. A manual by AGAR SCIENTIFIC LTD.
} distributed through PLANO recommends to bring the specimen from
} 70 % EtOH into LR White/70 % EtOH (2:1) before embedding in pure LR
} White. We find that this mixture very quickly separates into two
} phases. How shall we handle this?
} Furthermore the specimen seems to be cured insuffiently even after
} 24 hours of polymerisation at 55 C. Any comments on this?

I replied to this a few days ago, but am not sure that it arrived. So, again...

The 70% EtOH needs to be made up freshly just before use from 100%. Even
stored in a tightly stoppered bottle, the concentration of the EtOH seems
to decrease. This solved my similar problems.

70% EtOH seems to be the bare mininum that LR White will mix with; as soon
as the concentration drops below this there are problems with infiltration,
curing and the final blocks are brittle. I keep the LR White in the fridge
and use it without warming. All vials are rotated continuously during
processing. I embed the tissue in gelatin capsules in a normal embedding
oven. I rarely have problems with the blocks, though I must admit I would
only use LR White for immuno, never as a replacement for resin.

Diana van Driel
Dept Ophthalmology
Sydney University C09
AUSTRALIA 2006






From: alan.wilson-at-dsto.defence.gov.au (Alan Wilson)
Date: Thu, 1 Aug 1996 14:37:05 +1000
Subject: Re: Stray EM Fields

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High Frequency Earth Loops.
We also had an interesting stray fields problem which showed up in high
noise counts in X-ray spectra - the amount depending on the scan rate in
the SEM! This was traced to a high frequency earth loop (no DC connection)
with capacitive coupling associated with the thin plastic shin isolating
the X-ray detector from the column. This was solved by passing a few loops
of the whole cable going to the X-ray detector through a large toroid to
increase the high frequency impedance of this part of the loop.


Alan Wilson alan.wilson-at-dsto.defence.gov.au

Senior Research Scientist
Ship Structures and Materials Division
Aeronautical and Maritime Research Laboratory
Defence Science and Technology Organization
506 Lorimer St
Fishermens Bend 3207
Victoria Australia
ph 61 3 9626 7508, fax 61 3 9626 7816 or 61 3 9626 7087






From: Nebesarova LEM Motejl :      lem-at-paru.cas.cz
Date: Thu, 1 Aug 1996 08:24:06 +0100 (GMT+0100)
Subject: El.diffraction and CCD.

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Many best regards from the Czech Republic!

We are Lab. of El. Microscopy, Institute of Parasitology, Czech Academy
of Sciences. We also offer EM services to other Institutes and Universities
in the Czech in the field of biology. We want also to use electron
diffraction on the TEM Philips 420 and to connect CCD or TV camera to
microscop in the near future. And there are some problems to solve.

Please, I have a few questions.

1. Is there some difference in el. diffraction images obtained by
different way? I mean:
a/ Normal image is loaded by camera to PC and the diffraction is
obtained by computer.
b/ El.diffraction image is loaded on the planfilm and this one is
processed by laser diffraction.
c/ El. diffraction is loaded directly by camera and processed by computer.
d/ El. diffraction is loaded on the planfilm and this one is scanned
to computer and processed by computer.

2. Exist some camera /CCD or TV/, by which it is possible to load el.
diffraction images directly? / I'am afraid that very intensive main
beam can destroy the detector/

Thank You Very Much for responses.

Milos Motejl
Lab. of Electron Microscopy,
Institute of Parasitology,
Czech Academy of Sciences
Branisovska 31
370 05 Ceske Budejovice
CZECH REPUBLIC
FAX : 042/038/47743
E-mail: lem-at-paru.cas.cz
.............................................................................




From: ebs-at-ebsciences.com
Date: Thu, 1 Aug 1996 06:39:48 -0500
Subject: Re: TEM/LM flat bottom "beem" caps

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Dear Tobias,

Energy Beam Sciences carries the TAAB polypropylene embedding capsules. The
advantage of polypropylene is that these capsules can be autoclaved and used
at temperatures up to 100 degrees C. They come in in two diameters (6mm and
8mm) and two styles (flat ends and truncated pyramids). They can be found
on p45 of our Catalog 3, or, on-line, at http://www.ebsciences.com, in the
TEM supplies section of the catalog.

Best regards
Sonja L. White, Sales & Marketing Secretary
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: kna101-at-utdallas.edu
Date: Thu, 1 Aug 1996 07:41:09 -0500 (CDT)
Subject: Re: TEM/LM flat bottom "beem" caps

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Hello,

By some odd coincidence, I was just trying to find out if such a
thing exsited yesterday. Right now, I'm trying to make a beem capsule
work by setting it on it's cap and snipping the tip to allow filling from
what is now the top. I would love to hear if these flat bottom capsules
are available in the US too, so please post your replies to the list.
Thankyou,

Karen

On Wed, 31 Jul 1996, Tobias Baskin wrote:

} Greetings,
} We have been using some capsules that are like "beem" capsules,
} 8mm diameter and with a snap-on cap, only instead of having a cone or a
} pyramid, they are simply flat on the bottom. They are made from
} polyethylene (polypropylene ones are made too). These are great for flat
} embedding. We got ours from TAAB in England, but we have used them up
} nearly. Does anyone know if these are for sale in the USA? A quick perusal
} of catalogs from several major USA supply houses failed to find 'em. Thanks
} for any leads.
} Tobias Baskin
}
} - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
} ___ ____ ^ ____ _____ Tobias I. Baskin
} / \ / / \ / \ / University of Missouri
} / | / / \ / / Biological Sciences
} /___ / /__ /_____\ / /__ 109 Tucker Hall
} / / / \ ( / Columbia, MO 65211 USA
} / / / \ \ / voice: 573-882-0173
} / /____ / \ \____/ /_____ fax: 573-882-0123
}
}
}




From: Eugene Zarakhovsky :      ez1-at-netcom.com
Date: Thu, 01 Aug 1996 08:50:35 -0700
Subject: SEM - Problem with M6A Pirani Gauge Controller

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Message-Id: {2.2.32.19960801155035.006c12e8-at-192.0.2.2}
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I have a rather old Cambridge Stereoscan SEM, and I think the pirani gauge
controller may have gone bad. It's an Edwards pirani gauge, model M6A. If
anyone knows how to check whether it still works and/or where to find a
replacement (Edwards no longer makes that model), please e-mail me. Thank you.
-Eugene Zarakhovsky
ez1-at-netcom.com
"Show me an angel, I'll paint one." -Courbet





From: wesleysm-at-biology.und.ac.za (James Wesley-Smith)
Date: Thu, 1 Aug 1996 16:38:59 +0200
Subject: Measuring freezing rates

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Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za}

I would appreciate to hear from anyone who has had experience in
RECORDING freezing rates used in cryofixation. I am particularly
interested in establishing the duration and interval of the sampling
required.

Please reply directly to my address.

Thank you.


###################################################
James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa
http://www.und.ac.za/und/emu/emunit.html
###################################################




From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Thu, 01 Aug 1996 08:54:44 -0400
Subject: Re: Stray EM Fields

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Hi Bob. I will summerize all of the replies in a bit. Meanwhile,
there was another thread a while back posted to the server which I have
archived. Go to the web page listed at the end of this message. Click on the
"Tips & Tricks" button. You will find a link for "SEM Techniques &
Instrumentation" which wil point to another link called "Dealing with Drift,
SEM". It may prove useful. If you do not have web access, let me know and I
will be happy to get the info to you some other way.



At 09:44 AM 7/31/96 +0600, you wrote:
} } Date: Wed, 31 Jul 1996 08:31:36 -0700
} } From: John Best {jbest-at-vicon.net}
} } To: sdw-at-biotech.ufl.edu
} } CC: microscopy-at-Sparc5.Microscopy.Com
} } Subject: Re: Stray EM Fields
} }
} } Scott,
} }
} } This is an odd image. Typically, with periodic EM or mechanical
} } interference, you'll see jagged edges. The amplitude of the "jagginess"
} } increases proportionally with magnification.
} }
} } I assume in this image your referring to the banding on the substrate. Is
} } this correct?
} }
} } My best guess at this time if that the scan rotation was at 90 degrees
} } and you've left the SEM's ABC circuit on during image collection.
} }
} } John Best -- ELMDAS Co.
} } jbest-at-vicon.net
}
} We don't have Scott's problem with our JEOL 5400 SEM, but we do have this
} jagginess problem that John Best deicribes. JEOL has been here and we have
} looked for electrical fields. Not a problem. A shield was installed in the
} consol to eliminate fields emmanating from the CRT's. No effect The company
} service people are stumped, and we are left unable to use the SEM a high
mags.
} This outlet problem intrigues me, however. Please keep this thread going
for a
} while.
}
} Bob Schmitz
} rschmitz-at-uwspmail.uwsp.edu
} or
} rschmitz-at-macsrv1.uwsp.edu
} (note its macsrv"one" not "el")
} Robert (Bob) J. Schmitz
} Department of Biology,
} University of Wisc. Stevens Point.
} Stevens Point, Wisconsin 54481
} ph 715-346-2420
}
}




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 904-392-1295
ICBR EM Core Lab fax 904-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "





From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Thu, 01 Aug 1996 10:05:11 -0400
Subject: Venting Print Processors

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Message-Id: {s200817f.084-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

I apologize for bringing this subject up again, but we have recently
purchased a print processor and the information I was collecting from
the bulletin board has disappeared during my relocation to another
province. I was hoping that someone may have a summary of the
discussion about the venting of print processors as we are in the midst
of renovating our darkroom to install a new one. Please respond to my
E-mail address so that I don't clog up the bulletin board with repetitive
information.
Thanks in advance,
Paula.
allanwojtasp-at-em.agr.ca





From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Thu, 1 Aug 1996 14:36:31 -0400 (EDT)
Subject: O-Rings

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I was wondering if anybody knows of a supplier of o-rings in the metric size.
The suppliers I have dealt with here in Baltimore only have stock of even
size o-rings such as 2mm, 3mm, etc. I need a supplier that can provide
fractional sizes also such as, 2.3mm, 2.5mm, etc. I am more concerned
about the width of the o-ring being fractional than I am of the I.D. or O.D.
All of the o-rings I use are in whole numbers as far as the O.D. and I.D.
goes. Thanx.

O.D.O's

Phil




From: rw9-at-psu.edu (Rosemary Walsh)
Date: Thu, 1 Aug 1996 19:34:10 GMT
Subject: Re: suspend mail during vacations

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please unsuscribe

Rosemary Walsh

####################################################
Rosemary Walsh
Electron Microscope Facility for the Life Sciences
The Biotechnology Institute for Research and Education
1 South Frear Lab
University Park, PA 16802
814-865-0212 email:rw9-at-psu.edu
####################################################






From: Murphy, Judy :      murphy-at-sjdccd.cc.ca.us
Date: 1 Aug 1996 11:10:38 -0800
Subject: Printer Security

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Message-ID: {n1373224632.74684-at-sjdccd.cc.ca.us}

I have just purchased a Harris printer which produces black and white photo
quality prints on special but affordable paper (i.e.$ 0.68). My concern is
that one student could still break the budget. This will be attached over a
ether net to our Macs (Mac 8500 and Mac II). We have two printers hooked up
to the computers i.e. a Apple 600 dpi laser printer and soon the Harris
Printer. I only need the security on the Harris printer.

Does anyone know of a password entry to a printer which I might be able to
change. Even if I couldn't change it, just having a password entry would
help. I have checked with the folks that make MacControl, At East (Apple),
and On Guard which are security systems for Macs and they do not have a way of
doing that.

Any ideas would be appreciated.
Thanks in advance,
Judy Murphy

Judy Murphy, PhD
Dept. of Microscopy
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5649
e-mail: murphy-at-sjdccd.cc.ca.us





From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Thu, 1 Aug 1996 08:38:10 -0500
Subject: SET NOMAIL

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Subscribers....

Please refrain from telling people how to operate the
listserver using commands that DONOT exist on this
system.. I realize you are trying to give advice but please
READ your instructions. That is where you will find out
how to do things.

You all received instructions on Subscribing and Unsubscribing
when you initially subscribed and I periodically (a few times a year
repost those instructions to the current subscribers)
those are the commands that work no others. Using or telling people
to use commands that are NOT IMPLEMENTED do nothing more
than increase my work load.


Nestor
Your Tired & Friendly Neighborhood SysOp
who is also the Program Chairman of the Microscopy & Microanalysis 96 Meeting!!!






From: becks-at-sunynassau.edu (Steve Beck)
Date: Thu, 1 Aug 1996 11:04:16 -0500
Subject: Fall 1996 - TEM Course Announcement

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FALL 1996 COURSE ANNOUNCEMENT - Transmission Electron Microscopy (BIO. 221-E2)

NASSAU COMMUNITY COLLEGE

A fifteen week, fall 1996 semester, course in Biological Transmission
Electron Microscopy is being offered by the Biology Department of Nassau
Community College. This is a 4 credit course offered ONE EVENING PER WEEK,
Thursdays, starting at 5:30pm. Classes will begin on Sept. 5 and end on
Dec. 12, 1996.

This is a "hands-on" course emphasizing biological specimen preparation,
ultra-thin sectioning involving block trimming, glass knifemaking and
operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III),
thick and ultra-thin section mounting and contrast staining (UA and Pb
citrate), grid support films (formvar, carbon), student operation of the
TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs
through the process of black & white photography, and electron micrograph
analysis. Students will work on a chosen sample(s) with the goal of
producing a portfolio of high quality TEM photomicrographs of that
sample(s).

The course is widely transferrable and the cost per credit is reasonable at
$78 per credit.

More information about the Bio-Imaging Center at NCC, course descriptions
and syllabi, and the humble beginnings of a student gallery of EM
photomicrographs is available at our recently completed web site. The URL
is {http://www.sunynassau.edu/webpages/biology/becks.htm} . Any comments or
suggestions on the homepage would be appreciated - I'm somewhat new at
this!

For those without www access, the catalog description is specified below.
If you have further questions, you should e-mail me directly at the address
below.

Interested individuals should register early (prior to Aug. 15) since the
course is limited to a total enrollment of ten (10) students.
____________________________________________________________________________
____

CATALOG DESCRIPTION
BIO 221: Transmission Electron Microscopy -- 4 credits
Prerequisites: BIO 109-110 or equivalent, CHE 151-152 or equivalent.
An introduction to the basic principles of transmission electron
microscopy including tissue preparation, microscope (TEM) operation, black
& white photography, and micrograph interpretation. The entire laboratory
is devoted to the development of skills and preparative techniques involved
with the operation of an actual transmission electron microscope.
(3 lecture, 3 laboratory hours). Laboratory fee applies.
________________________________________________________________________________






Stephen J. Beck
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}





From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Thu, 1 Aug 1996 08:32:51 -0500
Subject: SET NO MAIL Is not Implemented!!

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Message-Id: {199608011327.IAA04242-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Listmembers,
} I don't know if this works on every list, but a technique I've used to stop
} emailbox overflow when I'm out of town is to use the following command:
}
} the TO: address should be LISTSERV-at-MSA.MICROSCOPY.COM
} in the text of the message put the line (no signature or footers):
} SET MICROSOPY NOMAIL
}
} When you're ready to get your mail again, send the following message to the
} listserv address (LISTSERV-at-MSA.MICROSCOPY.COM):
} SET MICROSCOPY MAIL
}
} It's probably about as effective as unsubscribing and resubscribing
} (provided you remember how to do that without sending your request to all of
} us list readers). It does have the advantage of avoiding the welcome
} messages that come when you resubscribe (not that we mind Nestor's eloquent
} prose...).
}
} Have a safe vacation.
}
} Doug
} .....................................................................
} : Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
} : Sr. Research Specialist University of Arizona :
} : (office: AHSC 4212A) P.O. Box 245044 :
} : (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
} : (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
} :...................................................................:
} http://www.pharm.arizona.edu/exp_path.html






From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Thu, 1 Aug 1996 14:36:26 -0600
Subject: flat bottom caps:summary

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Greetings,
I have had many replies to my earlier posting about "flat bottom
beem capsules". Thanks one and all.

1) There was a little confusion about "beem" capsules that have the normal
cone truncated into a kind of flat pyramid. This is not what I was asking
about. Instead, I use capsules with a completely flat bottom, as if you
turned the capsule upside down, and used its top for a flat surface (see
point two).

2) Several respondents weighed in with methods for do-it-your-self
solutions. For example, you can work with a normal "beem" capsule, snap the
top on, run a strip of parafilm or wax around the top and turn it upside
down. Or, you can cut off the conical bottom and put an extra top on the
"bottom", again sealing with wax or parafilm. If just a few samples are
needed, these methods are great, and we used to use them. But we process
dozens of samples a week (many weeks) and so all of this fabrication is a
drag.

3) It was suggested that capsules can be avoided altogether by using flat
embedding molds on top of which a second mold is placed, cavity side up,
which acts as an air-tight but uv transmittable barrier on top of the
samples.

4) Flat bottom polythene capsules, similar if not identical to the ones
from TAAB, are now sold by Ted Pella. Energy Beem Sciences apparantly also
can get them. EMS and SPI sell vials, perhaps the same as used for holding
em grids, that are 8mm in diameter, made of polythene, and apparently used
widely for flat embedding. I have no connection with any of these companies
other than as a satisfied customer.

Hope this helps,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: Jill Craig :      jcraig-at-unbc.edu
Date: Thu, 1 Aug 1996 15:20:58 -0700 (PDT)
Subject: graduate student projects

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Hi, I'm looking for some ideas of possible projects at the master's
degree level in SEM microscopy. Areas of primary interest would be
Materials Science/Engineering, Environmental, and Biological/Medical. I
know this is an incredibly diverse area. I'd just like to hear some
brainstorming type ideas on things you may have wanted to see
investigated, but time/money/etc. restraints don't allow it. I would be
happy to either post a summary or keep from posting any suggestions as
you prefer.

Thanks in advance for your ideas.


Jill




From: Frances Gallery [Neurobiology] :      fgallery-at-brain.bio.sunysb.edu
Date: Thu, 1 Aug 1996 14:37:04 -0500
Subject: (Fwd) Posting of TEM Course Announcement

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Dear Aspiring TEMists-
'Just thought I'd share this class info with you, should anyone be
interested; I took it last summer and found it a worthwhile intro
class!


FALL 1996 COURSE ANNOUNCEMENT - Transmission Electron Microscopy (BIO. 221-E2)

NASSAU COMMUNITY COLLEGE

A fifteen week, fall 1996 semester, course in Biological Transmission
Electron Microscopy is being offered by the Biology Department of Nassau
Community College. This is a 4 credit course offered ONE EVENING PER WEEK,
Thursdays, starting at 5:30pm. Classes will begin on Sept. 5 and end on
Dec. 12, 1996.

This is a "hands-on" course emphasizing biological specimen preparation,
ultra-thin sectioning involving block trimming, glass knifemaking and
operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III),
thick and ultra-thin section mounting and contrast staining (UA and Pb
citrate), grid support films (formvar, carbon), student operation of the
TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs
through the process of black & white photography, and electron micrograph
analysis. Students will work on a chosen sample(s) with the goal of
producing a portfolio of high quality TEM photomicrographs of that
sample(s).

The course is widely transferrable and the cost per credit is reasonable at
$78 per credit.

More information about the Bio-Imaging Center at NCC, course descriptions
and syllabi, and the humble beginnings of a student gallery of EM
photomicrographs is available at our recently completed web site. The URL
is {http://www.sunynassau.edu/webpages/biology/becks.htm} . Any comments or
suggestions on the homepage would be appreciated - I'm somewhat new at
this!

For those without www access, the catalog description is specified below.
If you have further questions, you should e-mail me directly at the address
below.

Interested individuals should register early (prior to Aug. 15) since the
course is limited to a total enrollment of ten (10) students.
____________________________________________________________________________
____

CATALOG DESCRIPTION
BIO 221: Transmission Electron Microscopy -- 4 credits
Prerequisites: BIO 109-110 or equivalent, CHE 151-152 or equivalent.
An introduction to the basic principles of transmission electron
microscopy including tissue preparation, microscope (TEM) operation, black
& white photography, and micrograph interpretation. The entire laboratory
is devoted to the development of skills and preparative techniques involved
with the operation of an actual transmission electron microscope.
(3 lecture, 3 laboratory hours). Laboratory fee applies.
________________________________________________________________________________




Stephen J. Beck
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu}
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}






From: ard-at-atom.ansto.gov.au (Arthur Day)
Date: Fri, 2 Aug 1996 09:47:32 +1000
Subject: Re: "high mag jaggies" (was Stray EM Fields)

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} Robert and all,
}
} In my experience, the "high mag jaggies" are most often caused by a
} mechanical vibration. Often the roughing pump is the problem. Sometimes
} a couple of tennis balls under the roughing pump will decrease the effect
} substantially. It's not always this simple though. Also check for cables
} touching the vacuum line from the RP. They can transmit these vibes.
}
} John

About a year ago we had an intermittent "high mag jaggies" problem on our
6300. Viewed at TV rate at } 50,000x the interference seemed to have a
fairly high frequency and underwent rapid variations in amplitude. Some
days it was there and other days it wasn't. It was indeed caused by a
mechanical vibration -- but in our case it was coming from the stage
motors, in particular the Z-axis motor. The effects of this vibration only
started to become visible above about 15-20,000x, and it could only just be
felt on some days by very lightly touching the motor once you already "knew
it had a vibration". Our Jeol engineer was the one who finally figured this
out and then he measured a ~2 volt sawtooth ripple on top of the DC holding
current to the stage motors. The problem was eliminated by changing some
resistors in the (non-Jeol) motor driver hardware, on the recommendation of
its vendors, to reduce the DC holding current and hence presumably the
absolute magnitude of the ripple that came with it. We have not had any
trouble since.






Arthur Day, Electron Microscope Unit http: //www.ansto.gov.au/
Ansto Materials Division Phone: 61-2-717-3457
PMB 1, Menai (Sydney), NSW, 2234 Fax: 61-2-543-7179
Australia Email: ard-at-atom.ansto.gov.au






From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Thu, 1 Aug 1996 14:49:18 -0500
Subject: Re: Measuring freezing rates

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Back when I was young, I tried measuring the rate of freezing of a thin
slice of tissue against a metal mirror surface at liquid nitrogen temp
using the quick freeze device now sold commercially as the "gentleman jim".
(for a description of the machine but not the freezing rate studies, see
Phillips & Boyne, J. Electron Microscopy Techniques 1:9-29 (1984). I built
a head for the tissue to rest on that had two electrodes on the bottom
surface. the slot in the head on which the tissue was placed was 195 um
deep and I used 300 um slides of tissue (liver or electrocytes). the
tissue completed the circuit and allowed current from a 6 V source to flow.
I added a 5.5 K ohm resister in series and measured a 1-2 V drop across
the resister so the tissue was presumably acting as a 6-10 K ohm. when the
tissue hit the metal mirror, the signal decreased continuously from the
moment of impact.. there was good reproducibility between slices. the
total event lasted approx. 2-300 msec with about 30% of the decay in the
initial 25 msec. even the initial 25 msec was biphasic with a very flat
response and a relatively slower decay. Heuser and Reese used an
alternative technique using capacitance. I think this material was
included as an appendix in their classic J Cell Biol (1979) 81:280 paper.
When I read that paper, I had problems with their assumptions (I am doing
this from a 15 year old memory, so I hope I don't have this wrong). They
assumed after the first molecular layer had frozen, the tissue could be
viewed as 2 dielectrics in series, one water dielectric and one ice. My
problem with this is that I viewed the ice as a dielectric and the saline
(unfrozen tissue) above as an extension of the copper electrode and
therefore I thought the saline was in fact acticing as the upper "plate" of
a capacitor. I seem to remember another problem with the maximum
breakdown voltage of a dielectric requires a very low applied voltage at
the frequencies Heuser et al (and Van Harreveld in earlier studies) had
used in their studies and I am not sure their studies took that into
account. It has been a long time since I thought about this but if you
want more specifics on what I did or read the Heuser paper and have
questions about my critique, I will try to figure out my old notes. good
luck.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: SOBOCIG :      sobocig-at-aa.wl.com
Date: Thu, 01 Aug 1996 10:13:09 -0400 (EDT)
Subject: Re: TEM/LM flat embedding with "beem" capsules

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For years, I have taken BEEM capsules, cut the tapered end off with a
razor blade, and embedded tissue flat on the 'lid'. After curing the blocks,
removing them is easy with a 'cherry pitter' device, which in one catalog the
BEEM Company refers to as a 'capsule press'. To remove the cured blocks, simply
open the 'lid' of the capsule where the tissue is, place it tissue-side down in
the press, and press down on the lever to extricate the block.
I find it hard to believe that this is an original idea, but hopefully
it will help some of you with flat-embedding dilemmas.


Gregg Sobocinski
Parke-Davis
Pharmaceutical Research Division
Ann Arbor, Michigan
USA
Sobocig-at-aa.wl.com

*** And I'm sure the lawyers would be happy if I include:
The views expressed above are my individual opinions and do not represent the
views or policies of Parke-Davis.





From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 1 Aug 1996 17:46:39 -0400 (EDT)
Subject: Re: El.diffraction and CCD.

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Dear Milos,

} We want also to use electron
} diffraction on the TEM Philips 420 and to connect CCD or TV camera to
} microscop in the near future. And there are some problems to solve.
}
} Please, I have a few questions.
}
} 1. Is there some difference in el. diffraction images obtained by
} different way? I mean:
} a/ Normal image is loaded by camera to PC and the diffraction is
} obtained by computer.

There are several limitations to resolution using this method. A
video camera has a limited pixel size and number--larger and fewer than
film--so the Fourier components are inherently fewer than for scanned film.
This may or may not be a problem. The intensities are also of limited range,
giving possible inaccuracies, and the intensity at one pixel can affect that
measured at adjacent pixels. Of course, the contrast transfer function also
affects the intensities. Finally, the resolution is limited by the lens
aberrations (assuming that pixel size does not impose a more stringent
limit). It is, however, very fast.

} b/ El.diffraction image is loaded on the planfilm and this one is
} processed by laser diffraction.

There are some limitations due to lens aberrations and (possibly)
grain size. I find typically that this method gives about one more order
than the first method. The difference is probably due to the greater sam-
pling frequency as a consequence of smaller pixel size.

} c/ El. diffraction is loaded directly by camera and processed by computer.

The resolution limits are usually much better than with the image.
In the very favorable cases, ED can go out to at least 0.05 nm. There can
be problems with intensity quantitation due to pixel size and crossover be-
tween pixels. The first problem is called "Wooster error"; Inoue's book on
video microscopy explains the second type of problem.

} d/ El. diffraction is loaded on the planfilm and this one is scanned
} to computer and processed by computer.

Resolution is equally good, but accurate quantitation requires
sensitometry--measuring a blackening curve (OD vs exposure)--which can be
tedious. The correct scanner to use is one with a small spot of light,
such as the Perkin-Elmer or Optronics. CCD array scanners, such as Eikonix
or CCD cameras will not account correctly for very intense spots due to
stray light from more transparent areas of the film. This is the most
accurate method, and the slowest.

}
} 2. Exist some camera /CCD or TV/, by which it is possible to load el.
} diffraction images directly?

Yes. I have seen Ken Downing's camera on the IVEM at Berkeley.

/ I'am afraid that very intensive main
} beam can destroy the detector/

A beam stop can be used to block the beam--I don't know what the
consequences are if the beam is not blocked, but I'd be worried too.
Yours,
Bill Tivol




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: 01 Aug 96
Subject: Ground loops and image defects

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Message-Id: {199608020347.XAA21834-at-mime2.prodigy.com}
X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc04

-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #3.0 ] --

Twice in the past twenty six years we had mysterious problems arise that
had all the symptoms of stray AC fields which caused various problems
with the images on what were then JEOL JSM U3 SEMs. In one instance in
particular, quite a bit of major equipment was brought in (and dollars
spent) to find the source of the field but to no avail. The proposed
"fixes", and with no guarantees, included extensive "mu" metal shielding
of the microscope room.

However, by going around and turning off individual circuits in the
building, and finding out which "circuit", when "off" caused the problem
to go away, in one instance it was traced to a ground feedback loop from
a vacuum pump with windings starting to fail. And in the other instance
, which was a little more challenging, it was traced to a compressor
motor that was part of the microscope itself, with (apparently) the same
problem, windings starting to fail on the motor. In both instances,
replacing the failing motor (or pump), the problem disappared.

So while expensive and complex solutions are sometimes necessary, don't
sell short what might be accomplished with the good old common horse
sense approach.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.
com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.
com


Look for us!
############################
WWW: http://www.2spi.com
############################
=====================================================




From: wesleysm-at-biology.und.ac.za (James Wesley-Smith)
Date: Fri, 2 Aug 1996 09:01:11 +0200
Subject: Re: Measuring freezing rates

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Comments: Authenticated sender is {wesleysm-at-biology.und.ac.za}

Hello Tom
Thanks a stack for the information. It certainly gives me a good
starting point. I am intersted in acquiring a data collecting card,
and they obviously vary in speed and frequency of the sampling (and price).
I just wish I could keep this sort of information in MY head for 15
years!!

Thanks again.


###################################################
James Wesley-Smith
Electron Microscope Unit
George Campbell Building
University of Natal
Durban, South Africa
http://www.und.ac.za/und/emu/emunit.html
###################################################




From: Karl Kadler :      KKADLER-at-fs1.sem.man.ac.uk
Date: Fri, 2 Aug 1996 14:43:58 BST
Subject: unsuscribe

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Message-Id: {m0umFai-0000fKC-at-ermail01.btx.dtag.de}

unsuscribe
Karl Kadler, PhD,
Wellcome Trust Senior Research Fellow,
Wellcome Trust Centre for Cell-Matrix Research,
Electron Microscope Unit,
School of Biological Sciences,
University of Manchester,
Stopford Biulding 2.205,
Oxford Road, Manchester M13 9PTAssistant: Carol McMurdo (cmcmurdo-at-fs2.scg.man.ac.uk)




From: Ron Neumeyer :      micron-at-bc.sympatico.ca
Date: Fri, 2 Aug 1996 07:15:47 -0700 (PDT)
Subject: Need Zeiss/Leitz Accessories

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I am looking for the following optics and accessories:

(1) Zeiss West dry darkfield condenser with holder Z (0.75/0.85)
(2) Zeiss West planopo 25x phase 3 objective
(3) Leitz Ortholux nosepiece

If you have any of the above in mint condition please advice quoting price,
including shipping to Vancouver Canada, in $US.

Thank you for your attention in this matter.

Regards,

Ron Neumeyer

phone 604-582-2552
fax 604-623-6239
E-mail (micron-at-bc.sympatico.ca)





From: A. Kent Christensen :      akc-at-umich.edu
Date: Fri, 2 Aug 1996 10:08:51 -0400 (EDT)
Subject: Re: Ground loops and image defects

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Sometimes these field effects can be very strange and challenging. Years
ago, when I was at Temple Univ. Medical School, in Philadelphia, a
colleague in the building had a recently installed Philips 300 TEM that
suffered from intermittent image deflection. Things were all right most
of the time, but occasionally the image would be deflected off to the side
for a time, and would then return to its normal position. The Philips
service personnel tried everything, but the cause remained a mystery. It
eventually turned out that an elevator in the vicinity had a large iron
counterweight that went up and down the shaft all day long. The image
deflection occurred when this magnetized mass of iron happened to be at
the same level as the microscope.

A. Kent Christensen, University of Michigan, akc-at-umich.edu.





From: Jun Wang :      g9326479-at-mcmail.cis.mcmaster.ca
Date: Fri, 2 Aug 1996 12:12:27 -0400 (EDT)
Subject: unsubscribe

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unsubscribe for vacation

******************************************************************************
Jun Wang g9326479-at-mcmail.cis.mcmaster.ca
Center for Electroptical Materials
And Devices/Dept. of Engineering Physics Tel. 905 525 9140 x24936
McMaster University Fax: 905 527 8409
Hamilton, Ontario Canada L8S 4L7





From: rml-at-med.unc.edu (Robert Bagnell)
Date: Fri, 2 Aug 1996 13:16:53 -0500
Subject: LEO (Zeiss) EM 906 Users

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Mime-Version: 1.0
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Are there any other EM 906 owners out there who would like to share
their experiences with this instrument?







From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 2 Aug 1996 12:37:30 -0400
Subject: RE-Stray EM fields

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Message-ID: {n1373132754.39014-at-mse.engin.umich.edu}

Subject: Time: 12:23 PM
OFFICE MEMO RE:Stray EM fields Date: 8/2/96

I fully agree with Chuck Garber. A few common sense tests can often clear up
the cause of image irregularities without invoking a lot of expensive
high-tech methods. We had one instance when the heating element in a
furnace in a lab down the hall had shorted over to ground, producing
horrendous ground loop currents through the heating ducts and steel girders
in the walls of the building that gave periodic image distortion. In
another instance, an educational TV station was feeding signals into the
building mains that caused problems.

However, if all else fails, there is a company (Linear Research
Associates, Trumansburg, NY 14886 Fx: 770-368-8256) that specializes in
correcting EM field problems. In fact, Curt Dunham, of LRA has recently
published a series of articles on EM field problems in "Microscopy Today".
You might contact him.






From: Woody.N.White-at-mcdermott.com
Date: 2 Aug 96 13:41:00 -0500
Subject: More vibes ~ ~ ~ ~ ~

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As the operator of a well used Etec, I have encountered worn and
loose gears/bearings in the stage. Enviro vibes, which normally
did not create a problem, caused "jaggies" as low as 5000x.

Etec FYI: My system has been modified by removing the diff pump and
installing a turbo. Beware of ball bearing units. The Etec is far
too susceptible to their vibrations. I have been using a Leybold
maglev pump since they became available and can see no pump induced
vibes below about 30Kx.

Woody White
Babcock & Wilcox Research Ctr.
woody.n.white-at-mcdermott.com

woody.white-at-worldnet.att.net




From: Sara Miller :      saram-at-acpub.duke.edu
Date: Fri, 2 Aug 1996 09:24:11 -0400 (EDT)
Subject: Re: Measuring freezing rates

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On Thu, 1 Aug 1996, James Wesley-Smith wrote:

} Date: Thu, 1 Aug 1996 16:38:59 +0200
} From: James Wesley-Smith {wesleysm-at-biology.und.ac.za}
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: Measuring freezing rates
}
} I would appreciate to hear from anyone who has had experience in
} RECORDING freezing rates used in cryofixation. I am particularly
} interested in establishing the duration and interval of the sampling
} required.
}
} Please reply directly to my address.
}
} Thank you.
}
}
} ###################################################
} James Wesley-Smith
} Electron Microscope Unit
} George Campbell Building
} University of Natal
} Durban, South Africa
} http://www.und.ac.za/und/emu/emunit.html
} ###################################################
Try contacting Dr.Joe Costello at
Dept. of Cell Biology
University of North Carolina
Chapel Hill, NC 27514

Sorry, I don't have an email address.

For faster answers, you might also check your library. He has published
info on rates of freezing in different cryogens; he may have described
the methods for measuring them. It was probably in the late 1980's.

Another possibility is to contact the companies that sell freezing
equipment such as slammers, etc. They should be able to give you some
references on "how they know their product works."

Good luck,
S.}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: Judy Ogilvie :      jmo-at-cidmac.wustl.edu
Date: 2 Aug 1996 15:35:30 +0100
Subject: Re: Venting Print Processors

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test-header: [jmo-at-cidmac.wustl.edu]
Message-ID: {n1373122315.51563-at-CIDMAC.wustl.edu}
"Paula Allan-Wojtas" {AllanWojtasP-at-em.agr.ca}
X-Mailer: Mail*Link SMTP-QM 3.0.2

I am new to the listserver and have missed any recent discussion of print
processors. We are likely to buy one in the near future and would appreciate
comments on what people have and like.

I really liked the old Kodak print processors. They were very fast, easy to
use, relatively easy to clean, and had a small footprint on the benchtop. But
they are no longer available as far as I can tell. The new ones that I have
looked into all seem to be designed for professional labs. Although the
prints come out completely fixed and dry, they take a relatively long time
which is a pain for test prints. Ours will get moderate and sporadic use so
cleaning out the chemicals should be easy.

Thanks in advance.
jmo

------------------------------------------------
Judith Mosinger Ogilvie, Ph.D.
Assistant Research Scientist
Central Institute for the Deaf
-affiliate of Washington University-
818 S. Euclid Ave.
St. Louis, MO 63110
tel: 314-977-0280
fax: 314-977-0030
e-mail: jmo-at-cidmac.wustl.edu
------------------------------------------------








From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 2 Aug 1996 11:58:21 -0400
Subject: RE-O-ring sizes

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Message-ID: {n1373135308.85486-at-mse.engin.umich.edu}

Subject: Time: 11:49 AM
OFFICE MEMO RE:O-ring sizes Date: 8/2/96

Phil:
I have always used O-rings in the Parker Series 2 that I obtain from the
Zatkoff Co. in Farmington Hills, MI (313-478-2400). These are based on
'nominal' widths which are common fractions of an inch; however, actual
widths don't correspond exactly to the nominal ones, and there is some
variation probably due to manufacturing variability (it's difficult to
measure O-rings exactly, anyway). Here are some of the standard nominal
sizes with the quoted corresponding actual dimensions:
Nom: 1/16": Act: 1.78 mm, 0.70"
3/32": 2.62 mm, 0.103"
1/8": 3.53 mm, 0.139"
3/16": 5.33 mm, 0.210"
1/4": 6.99 mm, 0.275"
For each thickness there is a range of ID sizes available.
I have just been designing a couple of devices for Japanese-built TEMs
(which one might expect to have metric O-rings, since all other dimensions
are metric), and find that all the O-rings used on them fall into this series
pretty nicely. Possibly you also can find O-rings from this series that
will meet your needs. Zatkoff stocks them in Viton, and several other kinds
of rubber.
Good luck, Wil Bigelow (bigelow-at-umich.edu)





From: bmenco-at-casbah.acns.nwu.edu (Bert Menco)
Date: Fri, 2 Aug 1996 16:38:07 -0500
Subject: Re: Measuring freezing rates

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Re. James Wesley-Smith's querie:

A good and recent source book:

(vacationing) Patrick Echlin, Low Temperature Microscopy and Analysis.
Plenum Press, New York , 1992

I think that Joe Costello joined Univ. North Carolina, Chapel Hill

Bert Menco
Neurobiology & Physiology
Hogan Hall
Northwestern University
Evanston, IL 60208-3520






From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Fri, 2 Aug 1996 11:47:47 -0400 (EDT)
Subject: vibrations

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Vibration can be a nasty problem. You can't assume they are coming from
your area. When I was at George Washington Univ. Med School my lab was
on the 5th. floor. Never had problems with vibration until one day I was
looking at cilia at 200,000 times and saw it. Zeiss came in to look at
the scope and the surrounding area and couldn't find what was causing the
problem. They saw vibration only when they put the meter on the desk of
the scope. Put it on the floor, no vibration detectable. We looked at
another TEM on the floor below us and they had no problem even at 300,000x.
After much hunting we found a air handling unit 2 floors below us and
about 100ft. away from the scope with a bad rubber foot pad. We had the
physical plant dept. replace it and our vibration problem disappeared.
Apparently this was causing low level vibration that my scope was picking
up and the other scope (floor below) wasn't. Vibration, especially low
level can be anywhere, construction several blocks away, bad feet on a
centrifuge, new equipment, just about anything. So if you're having a
problem with vibration check everything you can, don't rule anything out
unless you've checked it out, this includes anything touching your
mechanical pumps such as cables, hoses or even boxes you might have set on
the backing hoses.

Peace,

Phil




From: Mike Folsom :      mwfolsom-at-UNM.EDU
Date: Fri, 2 Aug 1996 12:21:04 -0600 (MDT)
Subject: Re: graduate student projects

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Message-Id: {m0umLNk-0004VWC-at-fast.net}

On Thu, 1 Aug 1996, Jill Craig wrote:

}
} Hi, I'm looking for some ideas of possible projects at the master's
} degree level in SEM microscopy. Areas of primary interest would be
} Materials Science/Engineering, Environmental, and Biological/Medical. I
} know this is an incredibly diverse area. I'd just like to hear some
} brainstorming type ideas on things you may have wanted to see
} investigated, but time/money/etc. restraints don't allow it. I would be
} happy to either post a summary or keep from posting any suggestions as
} you prefer.
}
} Thanks in advance for your ideas.
}
}
} Jill
}


One thing to consider is JOBS -

The market for biological microscopists is fairly bad right now and the
salaries for the jobs that are out there aren't much better.

On the other hard the job market in materials microscopy seems to be
much better and the salaries are probably much higher.

No doubt some on the list won't agree with me on this but we would all be
better off if the number of skilled microscopists out there was cut.
And, the easiest way to do that is to stop training 'em.

Michael






From: preid-at-rsmas.miami.edu (Pamela Reid)
Date: Fri, 2 Aug 1996 19:57:09 -0500
Subject: unsubscribe

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unsubscribe

__________
Dr. Pamela Reid
Research Associate Professor
University of Miami/RSMAS-MGG
4600 Rickenbacker Causeway
Miami, Fl 33149

email: preid-at-rsmas.miami.edu
phone (305) 361-4606
fax (305) 361-4632






From: Andrew Buechele :      andrew-at-RSRCH.VSL.CUA.EDU
Date: Fri, 2 Aug 1996 20:01:06 -0500 EST
Subject: Ultramicrotomy-Hard Materials

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I am planning to buy a reconditioned ultramicrotome (since I don't
have enough money available for a new unit) and diamond knife
in hopes of producing TEM specimens of leached glass. I would
appreciate hearing from anyone who has gone the "used instrument"
route to ultramicrotomy of hard materials. What kind of success did
you have? Are there things to look out for? etc.
Thanks in advance.
Andy Buechele
The Catholic University of America
409 Hannan Hall
Washington, D.C. 20064
(202) 319-4995 FAX: (202) 319-4469




From: A. Greene :      ablue-at-mail.io.com
Date: Fri, 2 Aug 1996 17:49:19 -0500 (CDT)
Subject: Re: O-Rings

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} Date: Fri, 02 Aug 1996 16:47:36
} To: rutledge phil {prutle1-at-gl.umbc.edu}
} From: "A. Greene" {ablue-at-mail.io.com}
} Subject: Re: O-Rings
}
} Hello Phil, I have been very pleased with American Seal, Inc. as a source
for o-rings. They are very responsive and have even overnighted o-rings to
my motel when I was out doing microscope service. Great people and
excellent stock. Their address is: 1242 Kress Street
} Houston, Texas 77020
} Phone 800/527-3151
} FAX 713/675-3618
}
}
} Alex Greene
} Scientific Instrumentation Services, Inc.
} Number 499, Post Office Box 19400
} Austin, Texas 78760
} Phone: 512/282-5507
} FAX 512/280-0702
}
}
} At 02:36 PM 8/1/96 -0400, you wrote:
} } I was wondering if anybody knows of a supplier of o-rings in the metric size.
} } The suppliers I have dealt with here in Baltimore only have stock of even
} } size o-rings such as 2mm, 3mm, etc. I need a supplier that can provide
} } fractional sizes also such as, 2.3mm, 2.5mm, etc. I am more concerned
} } about the width of the o-ring being fractional than I am of the I.D. or O.D.
} } All of the o-rings I use are in whole numbers as far as the O.D. and I.D.
} } goes. Thanx.
} }
} } O.D.O's
} }
} } Phil
} }
} }
}





From: A. Greene :      ablue-at-mail.io.com
Date: Fri, 2 Aug 1996 17:49:19 -0500 (CDT)
Subject: Re: O-Rings

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} Date: Fri, 02 Aug 1996 16:47:36
} To: rutledge phil {prutle1-at-gl.umbc.edu}
} From: "A. Greene" {ablue-at-mail.io.com}
} Subject: Re: O-Rings
}
} Hello Phil, I have been very pleased with American Seal, Inc. as a source
for o-rings. They are very responsive and have even overnighted o-rings to
my motel when I was out doing microscope service. Great people and
excellent stock. Their address is: 1242 Kress Street
} Houston, Texas 77020
} Phone 800/527-3151
} FAX 713/675-3618
}
}
} Alex Greene
} Scientific Instrumentation Services, Inc.
} Number 499, Post Office Box 19400
} Austin, Texas 78760
} Phone: 512/282-5507
} FAX 512/280-0702
}
}
} At 02:36 PM 8/1/96 -0400, you wrote:
} } I was wondering if anybody knows of a supplier of o-rings in the metric size.
} } The suppliers I have dealt with here in Baltimore only have stock of even
} } size o-rings such as 2mm, 3mm, etc. I need a supplier that can provide
} } fractional sizes also such as, 2.3mm, 2.5mm, etc. I am more concerned
} } about the width of the o-ring being fractional than I am of the I.D. or O.D.
} } All of the o-rings I use are in whole numbers as far as the O.D. and I.D.
} } goes. Thanx.
} }
} } O.D.O's
} }
} } Phil
} }
} }
}





From: zimarie-at-dibit.hsr.it (Zimarino Vincenzo)
Date: Sat, 3 Aug 1996 16:11:45 -0300
Subject: Re: O-Rings

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subscribe microscopy
vujanam-at-dibit.hsr.it (Milos Vujanac)








From: zimarie-at-dibit.hsr.it (Zimarino Vincenzo)
Date: Sat, 3 Aug 1996 16:08:29 -0300
Subject: Re: O-Rings

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Mime-Version: 1.0
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"subscribe microscopy" zimarie-at-dibit.hsr.it

Vincenzo Zimarino
San Raffaele Scientific Institute
DIBIT- Biological and Technological
Research Department
Room 4-A2-46
Via Olgettina, 58
20132 Milano Italy
Tel. + 39 2 26 43 48 96
Fax + 39 2 26 43 48 44
e-mail : zimarie-at-dibit.hsr.it






From: Murphy, Judy :      murphy-at-sjdccd.cc.ca.us
Date: 3 Aug 1996 12:28:24 -0800
Subject: Web Disc Groups

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Message-ID: {n1373047125.48457-at-sjdccd.cc.ca.us}

We are teaching microscopy and I want to make discussion groups for each of my
classes using the internet. I would appreciate anyone's experience (good and
bad) in the software they have used and problems that I might encounter.
Thanks in advance
Judy M.

Judy Murphy, PhD
Microscopy Technology Center
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5600
e-mail: murphy-at-sjdccd.cc.ca.us
program web page: http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html





From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Sat, 3 Aug 1996 18:57:26 -0400
Subject: Re: UNSUBSCIBE

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In-Reply-To: {66C8033201F70300-at-mhs.unc.edu}

UNSUBSCRIBE





From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 04 Aug 96 12:48:22 EDT
Subject: Tripod Polisher (R) Workshop

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REDUCED FEE REGISTRATION DEADLINE AUGUST 31, 1996

Workshop on Tripod Polishing

Workshop Objective
This course will cover all aspects of pre-thinning and focus on final thinning
via Tripod Polishing. Due to the limited class size and the extensive hands-on
opportuinities, this course is well suited to novices as well as advanced
Tripodders. The course will include sections on:

How to do it and why should I?
What's really going on and what am I really seeing?
How to prepare small, specific area cross-sections.
The problem of wildly differing materials (eg tungsten).
Rapid preparation of TEM cross-sections.
Preparation of a wide range of materials: semiconductors, ceramics, metals,...

Hands-on Opportunity
This course will be unique in that it will provide a hands-on opportunity for
every class participant. Tripod Polishers, Polishing Wheels, and pre-thinning
equipment will be made available to all participants and actual samples will be
prepared - by the students - as part of the course. This is a great opportunity
to get your hands dirty and actually learn by doing. The instructors will walk
you through each step of the process and then let you loose on the equipment.
This course is designed to teach the Tripod Polishing technique. Silicon
samples will be provided to the students and used as the basis for the course
teaching.

Workshop Location and Dates
South Bay Technology - San Clemente, CA
Dates: Friday & Saturday - October 18-19, 1996

Previous Participants (partial list)
INTEL, AMD, Motorola, LSI Logic, Conner Peripherals, Univ of Maryland, Univ of
New Mexico, UNAM (Mexico), LG Electronics (Korea), Battelle, MEMC, MVA Inc.,
Univ of Michigan, U.S. Bureau of Mines, IBM, Naval Research Lab, Purdue Univ,
Univ of Alabama, Univ of Arizona, Univ of Colorado, Univ of Wisconsin.

Class Size
Due to the intensive hands-on aspects of this course, class size will be
strictly limited to 10 participants.

Registration Fee: $795 (includes lunches and Friday night Dinner)
$695 if registration fee paid by August 31, 1996 Workshop

Registration Deadline: 30 days prior to workshop

For additional Information: Monica Pflaster
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673
TEL: 800-728-2233
FAX: 714-492-1499
e-mail: sbt-at-southbaytech.com




Registration Form

To register for the workshop, please fill out this form and send it, with
registration fee to:

South Bay Technology, Inc.
Workshop on Tripod Polishing
1120 Via Callejon
San Clemente, CA 92673 USA

Payment must be made in the form of a check, money order, Visa or MasterCard.
Checks must be drawn on a U.S. Bank and made payable to South Bay Technology,
Inc. Credit card orders by FAX may be sent to South Bay Technology at
714-492-1499.

Name:


Affiliation:


Address:




City: State:
Zip: Country:_________
Telephone: FAX:

e-mail:________________________
Primary sample type:




VISA MasterCard Card #_________________________________

Expiration Date________ Signature of Cardholder_________________________

Cardholder name (Please print):________________________________________





From: OSRAMWG-at-aol.com
Date: Sun, 4 Aug 1996 14:58:13 -0400
Subject: OSRAM HBO-lamps

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Dear subscribers,

first of all, let me introduce myself. My name is Dr. Wolfgang Gottschalk
(osramwg-at-aol.com) and I am the productmanager at OSRAM GMBH headquarters for
HBO and low wattage XBO lamps.
Last week it has come to my attention, that several people had problems with
lamps from our company.
Customers satisfaction is our goal and therefore we want to fix existing
problems as soon as they have been reported to us and also to support
customers if the problem is related to non-OSRAM equipment.
However up to now, only two cases have been reported to us. Therefore I like
to ask everybody, who had problems with our lamps to report this to me
directly. It will help us for the investigation to have the following
information:

1.) What was the problem?
2.) What type of lamp was it related to?
3.) In what type of equipment do you use our lamps? Is it modified?
4.) What was the serial number of the lamp (imprinted on the metal base)?
5.) To whom and when did you report the problem (local OSRAM office or
mircoscope equipment manufacturer)?

Please adress this information to me as soon as possible.
We will provide statements on the evaluation process in the microscopy list.
Please feel free to contact me in the future directly in case of any question
or problems. We feel, that an honest relationsship between manufacturer and
the customer is a win-win -relationship for both parties.

Best regards


Dr. W. Gottschalk




From: Murphy, Judy on Sat, Aug 3, 1996 12:28 PM
Date: 4 Aug 1996 11:51:04 -0800
Subject: Web Disc Groups

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Message-ID: {n1372963003.10258-at-sjdccd.cc.ca.us}


I sent out a general request for information on setting up discussion groups
for my classes on the internet the other day however from my responses I made
the question too general, so here is a try at making it more specific:

We are teaching microscopy and I want to make discussion groups for each of my
classes using the internet. Each of the discussion groups would have a
password. Having this on the internet would allow all the students in the
classes to get into the discussion whenever they wanted. They would also be
using the internet for research searches, etc. I would also like a way to
post class assignments etc, i.e. some sub group which the students don't
necessarily add anything to.

I would appreciate anyone's experience (good and bad) in the software they
have used and problems that I might encounter.
Thanks in advance
Judy M.

Judy Murphy, PhD
Microscopy Technology Center
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5600
e-mail: murphy-at-sjdccd.cc.ca.us
program web page: http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html



________________________________________________________

We are teaching microscopy and I want to make discussion groups for each of my
classes using the internet. I would appreciate anyone's experience (good and
bad) in the software they have used and problems that I might encounter.
Thanks in advance
Judy M.

Judy Murphy, PhD
Microscopy Technology Center
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5600
e-mail: murphy-at-sjdccd.cc.ca.us
program web page: http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html





From: John Best :      jbest-at-vicon.net
Date: Sun, 04 Aug 1996 17:56:02 -0700
Subject: Re: graduate student projects

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Message-ID: {320546A2.1360-at-vicon.net}

Michael Folsom recently wrote:
One thing to consider is JOBS -

The market for biological microscopists is fairly bad right now and the
salaries for the jobs that are out there aren't much better.

On the other hard the job market in materials microscopy seems to be
much better and the salaries are probably much higher.

No doubt some on the list won't agree with me on this but we would all be
better off if the number of skilled microscopists out there was cut.
And, the easiest way to do that is to stop training 'em.
End of Mr. Folsoms reply.

To Mr. Folsoms reply:
I apologize in advance to the subscribers of this listserver for
responding to an issue of this nature, but your response is disgusting,
Mr. Folsom.

In my view the Scanning Electron Microscope has been one of the primary
instruments contributing to the advancement of our economy, technology,
and the ability to raise the standard of living for all people.
Advancing the utilization of the SEM into new areas of research is
essential to our future. We need more people trained in the fundamental
physics which govern the limitations and possibilities for this
instrument. We also need people to excercize their imagination with
respect to the huge number of problems the SEM can be utilized to solve.

Ultimately this will create more jobs.

Respectfully
John W. Best.





From: VINCENT CHAND :      v.chand-at-student.qut.edu.au
Date: Mon, 05 Aug 1996 13:35:45 +1000 (EST)
Subject: comparison with TEM and SEM techinques (fwd)

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I need some information to general procedures w.r.t. prep. to specimens to
be studied under either TEM or SEM techinques. Can anyone help me with me
in this regard, as I am relative new to some of these procedures.

Basically I would like to any new techniques used to prepare specimens for
SEM and TEM.


TIA






From: Mike Folsom :      mwfolsom-at-UNM.EDU
Date: Mon, 5 Aug 1996 00:44:17 -0600 (MDT)
Subject: Re: graduate student projects

Contents Retrieved from Microscopy Listserver Archives
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} } Michael Folsom recently wrote:
} } One thing to consider is JOBS -
} }
} } The market for biological microscopists is fairly bad right now and the
} } salaries for the jobs that are out there aren't much better.
} }
} } On the other hard the job market in materials microscopy seems to be
} } much better and the salaries are probably much higher.
} }
} } No doubt some on the list won't agree with me on this but we would all
} } be better off if the number of skilled microscopists out there was cut.
} } And, the easiest way to do that is to stop training 'em.
} End of Mr. Folsoms reply.
}
} To Mr. Folsoms reply:
} I apologize in advance to the subscribers of this listserver for
} responding to an issue of this nature, but your response is disgusting,
} Mr. Folsom.
}
} In my view the Scanning Electron Microscope has been one of the primary
} instruments contributing to the advancement of our economy, technology,
} and the ability to raise the standard of living for all people.
} Advancing the utilization of the SEM into new areas of research is
} essential to our future. We need more people trained in the
} fundamental physics which govern the limitations and possibilities for
} this instrument. We also need people to excercize their imagination with
} respect to the huge number of problems the SEM can be utilized to solve.
}
} Ultimately this will create more jobs.
}
} Respectfully
} John W. Best.

I'm really not interested in letting this whole thing get personal -

I posted my reply because I thought a discussion about the job market for
microscopists would be a good thing.

A few thoughts late Sunday night..................

First, let's deal with the real - not the ideal. The simple fact is that
all kinds of wonderful microscopy needs to be done ranging from basic
paraffin work to SEM, TEM, & LSCM. However, that doesn't mean that
the system is gonna provide the funds or opportunity to do the work.

Hasn't anybody noticed that microscopy labs are being closed down or simply
allowed to decay all over the place. At a time when all kinds of incredible
technological developments are occurring in biological microscopy it seems to
be dying right before our eyes.

To blindly advise a student to follow us into the abyss is immoral. The job
market in the sciences stinks. And, as I hear it - its worse in Physics than
in Biology. So, what do we gain by telling somebody to "follow us" when we
can't even see a future for ourselves. Isn't this the ultimate act of
selfishness? To advise a student to "do what we did" and not care about
their future? Are they just sacrifical lambs on the alter of science? A
cheap and willing pair of hands to exploit for our personal good? Should
they be told to give up all matter of things for an uncertain future - all in
the cause of science!

Can't we do better than that?

Frankly, there will always be the need for a good microscopist to be handy.
But as a technician - not as a collegue. In a University setting that
automatically means a second class citizen with a third rate pay scale. Why
should we wish that on our students, our young collegues?


As, I said earlier I posted my original reply to start a discussion. We
really have no need for silly polemics.


Michael





From: Henrik Fermin :      henrik.fermin-at-fujifilm.se
Date: Mon, 05 Aug 1996 11:44:53 +0200
Subject: Re: graduate student projects

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subscribe henrik.fermin-at-fujifilm.se
****************************************************************************
Henrik Fermin Phone: + 46 (08) 729 14 57
FUJI FILM SVERIGE AB
Technical Manager BAS Fax: + 46 (08) 33 71 29 Box 23086
Medical Imaging System Dept.
104 35 Stockholm SWEDEN


*****************************************************************************





From: NEAL.SHARPE-at-spcorp.com (NEAL SHARPE)
Date: Mon, 5 Aug 1996 07:42:16 -0400
Subject: unsuscribe

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Unsuscribe

Thanks!!




From: Microscopy-request
Date: Monday, August 05, 1996 1:35PM
Subject: comparison with TEM and SEM techinques (fwd)

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I need some information to general procedures w.r.t. prep. to specimens to
be studied under either TEM or SEM techinques. Can anyone help me with me
in this regard, as I am relative new to some of these procedures.

Basically I would like to any new techniques used to prepare specimens for
SEM and TEM.


TIA






From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Mon, 5 Aug 1996 09:54:55 -0500
Subject: Re: Web Disc Groups-More Specific/Resources

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Although hypertext provides for microscopy the attractive benefit of
combining images with text, nothing useful for discussion groups is yet
available. This summary comes from the following URLs:

"Update Notes for Conferencing on the Web"
http://freenet.msp.mn.us/~drwool/webconup.html.

A large list of free and commercial resources with short evaluations and
demo sites are at:

"Computer Conferencing on the Web (Discussion Forums and Groupware)"
http://freenet.msp.mn.us/~drwool/webconf.html#wbb

Does anyone has experience with any of the programs cited in the above URs?
Best regards, Klaus

*******************
} I want to make discussion groups for each of my
} classes using the internet. Each of the discussion groups would have a
} password. Having this on the internet would allow all the students in the
} classes to get into the discussion whenever they wanted. They would also be
} using the internet for research searches, etc. I would also like a way to
} post class assignments etc, i.e. some sub group which the students don't
} necessarily add anything to.
******************

******************************************************************************
* Klaus-Ruediger Peters, Ph.D. :Molecular Imaging Laboratory *
* Director, Molecular Imaging Laboratgory : http://panda.uchc.edu/ *
* Biomolecular Structure Analysis Center : htklaus/index.html *
* University of Connecticut Health Center : *
* 263 Farmington Ave. :F r e e Access to Differential *
* Farmington, CT 06030-2017; U.S.A :Contrast Software at *
* e-Mail: Peters-at-BSAC.UCHC.EDU : http://panda.uchc.edu/ *
* Tel: (860) 679-3977; Fax: (860) 679-1989 : htklaus/DHP-Img.html#Software*
******************************************************************************







From: kdj928-at-lulu.acns.nwu.edu (Kevin Johnson)
Date: Mon, 5 Aug 1996 09:25:44 -0500
Subject: POSTDOCTORAL POSITION OPEN

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================================================================================

POSTDOCTORAL POSITION OPEN - IMMEDIATELY !!

ANALYTICAL ELECTRON MICROSCOPY/MATERIALS SCIENCE

Qualifications: A PhD in Materials Science/Physics or related area. Strong
experimental and conceptual background in interface structure and crystal
defect phenomena, especially in oxide materials is necessary. Experience in
oxide superconductors is desirable but not required.
Extensive hands-on experience in advanced analytical EM (XES, EELS,
CBED etc..) is a must, including TEM specimen preparation of complex
oxides, especially cross-sections of thin films.
The position is a part of a multi-faceted research program
concerned with structure-property correlation for oxide interfaces. The
research would involve experimental and phenomenological studies of oxide
interfaces in electroceramics, including high Tc compounds and
ferroelectric thin films. The principal theme is to exploit high spatial
resolution analytical techniques to probe the crystallography, chemistry
and aspects of electronic structure associated with internal interfaces in
these functional materials to complement ongoing atomistic simulation and
ab-initio electronic structure calculations of interfaces.
Instrumentation available at NU in the newly restructured Electron
Probe Instrumentation Center (EPIC) includes: A Hitachi HF-2000 FEG
TEM/STEM with x-ray, EELS, in-situ IV/LHe holder and e- holography set-up,
a Hitachi S4500-II FEG SEM with x-ray, EBSP/OIM and LHe stage with in-situ
IV probes, a Hitachi H8100 cTEM, and access to various other TEMs/SEMs and
other analytical instrumentation.

The position is open immediately for at least one year, and
renewable upon mutual consent. Northwestern University is an equal
opportunity employer.

*******************************************************
(Vinayak P. Dravid)
Associate Professor, Materials Science & Engineering
Director, Electron Probe Instrumentation Center (EPIC)
2225 N. Campus Drive, MLSF Building
Northwestern University, Evanston, IL 60208, USA
Ph.: (847) 467-1363, Fax: (847) 491-7820
E-mail: v-dravid-at-nwu.edu
*******************************************************






From: hou-at-kcgl1.eng.ohio-state.edu (Vincent D.H. Hou)
Date: Mon, 05 Aug 1996 15:11:13 -0500
Subject: Re: graduate student projects

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} } John W. Best recently wrote:
}
} In my view the Scanning Electron Microscope has been one of the primary
} instruments contributing to the advancement of our economy, technology,
} and the ability to raise the standard of living for all people.
} Advancing the utilization of the SEM into new areas of research is
} essential to our future. We need more people trained in the
} fundamental physics which govern the limitations and possibilities for
} this instrument. We also need people to excercize their imagination with
} respect to the huge number of problems the SEM can be utilized to solve.
}
} Ultimately this will create more jobs.


} } Michael Folsom recently wrote:
}
} First, let's deal with the real - not the ideal. The simple fact is that
} all kinds of wonderful microscopy needs to be done ranging from basic
} paraffin work to SEM, TEM, & LSCM. However, that doesn't mean that
} the system is gonna provide the funds or opportunity to do the work.
}
} Hasn't anybody noticed that microscopy labs are being closed down or simply
} allowed to decay all over the place. At a time when all kinds of incredible
} technological developments are occurring in biological microscopy it seems to
} be dying right before our eyes.
}
} To blindly advise a student to follow us into the abyss is immoral. The job
} market in the sciences stinks. And, as I hear it - its worse in Physics than
} in Biology. So, what do we gain by telling somebody to "follow us" when we
} can't even see a future for ourselves. Isn't this the ultimate act of
} selfishness? To advise a student to "do what we did" and not care about
} their future? Are they just sacrifical lambs on the alter of science? A
} cheap and willing pair of hands to exploit for our personal good? Should
} they be told to give up all matter of things for an uncertain future - all in
} the cause of science!
}
} Can't we do better than that?
}
} Frankly, there will always be the need for a good microscopist to be handy.
} But as a technician - not as a collegue. In a University setting that
} automatically means a second class citizen with a third rate pay scale. Why
} should we wish that on our students, our young collegues?
}

I would like to throwing in my two cents here:

Real or Ideal? This is a difficult question to answer. It may well be the
only question needed to be answered for your life.

So what should we tell these young/bright graduate students? The truth. I
belive that every one who has been doing research, either in basic or
applied research in any discipline, would agree that it is a very
interesting and exciting thing to do. I also believe that the excitment may
also be worn out with the increasing pressure to satisfy the "real" need of
life. So, new graduate students should be well informed before they make
their OWN decision. It's definitely unfair just to tell the one-side story.

I am still tring to find a balance between "real" and "ideal".


Vincent






From: John Best :      jbest-at-vicon.net
Date: Mon, 05 Aug 1996 16:00:54 -0700
Subject: Re: graduate student projects

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Message-ID: {32067D26.516E-at-vicon.net}

Mr. Folsom,

Nothing personal was intended, and I certainly didn't imply that students
should follow blindly. I simply don't see the present and future of SEM
as bleak in the slightest.

Salaries for microscopists might not be what you or I would like them to
be. If a student of microscopy derives their feelings of self worth from
their salary, then perhaps they shouldn't be looking at microscopy as a
profession. On the other hand, the majority of jobs in microscopy are
with universities and serious corporations. Perhaps salaries are
(arguably) low, but other important benefits are provided.

For now, I'll stick with my opionion that SEM based R&D at the university
level will ultimately expand the possibilities for electron beam
applications in industry, hence creating more opportunities. Go for it
graduate students. You might not want to be a microscopist when you come
out into industry, but the SEM will provide you with a unique view of
what makes things tick. The understanding that goes along with this
vantage point is invaluable to potential employers.

Regards and Good Luck to all.
John Best.






From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Mon, 5 Aug 1996 09:39:20 -0500
Subject: Re: graduate student projects

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} Can't we do better than that?
}
} Frankly, there will always be the need for a good microscopist to be handy.
} But as a technician - not as a collegue. In a University setting that
} automatically means a second class citizen with a third rate pay scale. Why
} should we wish that on our students, our young collegues?
}
}
} As, I said earlier I posted my original reply to start a discussion. We
} really have no need for silly polemics.
}
}
} Michael

My $0.02: John Best's idealism is laudable and easily defensible.
Unfortunately Michael Folsom has a much more realistic view of the world.
With one exception: it's the techs who are getting cut. I speak from
personal experience, having had 2 jobs now shot out from under me by
administrators who are more interested in personal advancement than it
serving the university community.
The problems of microscopy labs being starved into extinction and
closed is very real, and the only things now that prevent it is either lots
of press coverage or powerful administrators/full faculty who need
microsopy for their own purposes.
It all comes down to education: educating administrators,
legislators, and faculty about the value and need of all forms of
microscopy.
Until that happens, Michael Folsom's comments must be taken very
seriously. Too many students are being trained without regard for whether
or not they will be able to find any position, much less a good one.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********






From: VITHAGNA KHAMMANIVONG :      v.khammanivong-at-student.qut.edu.au
Date: Tue, 06 Aug 1996 14:30:10 +1000 (EST)
Subject: electron microscopy of bacteriophage

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Could you please send me book references or journal references on how to
process and view bacteriophage in a bacterial culture.





From: VITHAGNA KHAMMANIVONG :      v.khammanivong-at-student.qut.edu.au
Date: Tue, 06 Aug 1996 14:40:05 +1000 (EST)
Subject: electron microscopy of bacteriophage

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Could you please send me any book references or journal references on how
to process and view bacteriophage in a bacterial culture.





From: MATTHEW JOHNSTON :      n1414194-at-sparrow.qut.edu.au
Date: Tue, 06 Aug 1996 14:39:36 +1000 (EST)
Subject: Preparation of mucosal parasites

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Does anyone know of any good books on how to prepare giardiasis for the
electron microscope or on how to prepare a mucosal parasite, or on
waterborne parasites? Thanks.






From: em-at-mediacity.com (Ed Monberg)
Date: Tue, 6 Aug 1996 00:07:21 -0700
Subject: Re: graduate student prospects

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} Mr. Folsom,
}
} Nothing personal was intended, and I certainly didn't imply that students
} should follow blindly. I simply don't see the present and future of SEM
} as bleak in the slightest.



Dear Group,


A small point if I may:

What is the time constant for changes in the practitioner/demand ratio for
a given profession such as microscopy which has a longish "gestation" or
training period ?

Judging from other fields with similar training periods, the cycle is
shortest where the variables are strictly economic, such as in the various
branches of science, teaching and engineering. However where some
organization, the AMA or national bar association for example, twiddles the
process through lobbying, propaganda, disinformation in the marketplace, or
manipulation of demand for services, it appears that the cycles are much
longer, and the swings much deeper.

I vote for letting INFORMED self-interest do the strolling through the
yellow pages.



Ed Monberg
Palo Alto






From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: Tuesday, August 13
Subject: FREE Tripod Polisher Tutorial at MSA

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As part of the Microscopy & Microanalysis sponsored Exhibitor Tutorial Program,
South Bay Technology will be presenting a tutorial on Tripod Polishing for SEM
and TEM Analysis.

South Bay Technology Booth #500

Registration:
Please register at the MSA Education Committee Booth on the exhibit floor.
There is NO CHARGE for the tutorial.

If you need any additional information about the tutorial, please check the MSA
Web Site at:

http://www.msa.microscopy.com

or contact me directly. The first 10 people who confirm their registration by
visiting the South Bay Technology booth will receive a FREE Tripod Polisher (r)
T-shirt!

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
http://www.southbaytech.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: simkin-at-egr.msu.edu (Benjamin - Simkin)
Date: Tue, 6 Aug 1996 00:20:34 -0400
Subject: Microscopy in grad research

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As further contribution to the debate at hand:

I don't believe at all that one must make the choice to "be a
microscopist" or "not be a microscopist"; I am currently finishing up
my Master's on some applications of a microscopy techneque to certian
materials science type problems. This could certianly place me within
the catagory of "microscopist" from a number of standpoints, but I have no
intention of limiting myself to only one field of work in the future.

I am of the opinion that most people pursuing an advanced degree in materials
science should be able to use well such a versitile analytical tool, and
know the means behind the information that they are aquiring; otherwise
the microscope fails to provide all the information possible, and of the
questions that might be profitably asked, some would go unseen. While I believe
a good technician can (and should) come up with and answer a number of useful
questions for most any sample, it remains impossible to have anyone other than
the researcher in question to ask all that should be asked. Furthermore, without
a passible working knowledge of the principals of the type of microscopy being
employed, the researcher won't even know of the possible methods of answering
those questions that emerge during examination.

A minimum requirment would therefore seem to include knowledge of: the vacuum
system; beam-sample interactions, especially those that produce exploitable
contrast; and the exploitable signals arising from the above. Throw in there
rudimentary operation skills for the most common techniques, and one would seem
to have a microscopist, if perhaps not of terribly high skill. I would
therefore urge anyone in graduate study to at least examine projects dealing
with microscopy, as it will likely prove useful later on, and is unlikely
to hurt.

Ben Simkin (simkin-at-egr.msu.edu)
Dept. Mat. Sci. & Mech.
Michigan State University
o
previously some stuff that has been said included:

} So what should we tell these young/bright graduate students? The truth. I
} belive that every one who has been doing research, either in basic or
} applied research in any discipline, would agree that it is a very
} interesting and exciting thing to do. I also believe that the excitment may
} also be worn out with the increasing pressure to satisfy the "real" need of
} life. So, new graduate students should be well informed before they make
} their OWN decision. It's definitely unfair just to tell the one-side story.
}
} Vincent
}
}
} Salaries for microscopists might not be what you or I would like them to
} be. If a student of microscopy derives their feelings of self worth from
} their salary, then perhaps they shouldn't be looking at microscopy as a
} profession. On the other hand, the majority of jobs in microscopy are
} with universities and serious corporations. Perhaps salaries are
} (arguably) low, but other important benefits are provided.
}
} For now, I'll stick with my opionion that SEM based R&D at the university
} level will ultimately expand the possibilities for electron beam
} applications in industry, hence creating more opportunities. Go for it
} graduate students. You might not want to be a microscopist when you come
} out into industry, but the SEM will provide you with a unique view of
} what makes things tick. The understanding that goes along with this
} vantage point is invaluable to potential employers.
}
} John Best.




From: Betty Loraamm :      loraamm-at-chuma.cas.usf.edu
Date: Tue, 06 Aug 1996 08:52:38 -0500
Subject:

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Message-ID: {32074E26.246D-at-chuma.cas.usf.edu}

subscribe microscopy loraamm-at-chuma.cas.usf.edu




From: Stanley Hayes :      SHAYES-at-atlas.niaid.nih.gov
Date: Tue, 6 Aug 1996 10:16:24 -0400
Subject: old address

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Message-Id: {c=US%a=_%p=NIH%l=ATLAS-960806141624Z-661-at-atlas.niaid.nih.gov}

Please unsubscribe old address and subscribe me at this new address.
Thank you.

The new address is Stanley_Hayes-at-NIH.GOV




From: John Best :      jbest-at-vicon.net
Date: Mon, 05 Aug 1996 16:00:54 -0700
Subject: Re: graduate student projects

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Mr. Folsom,

Nothing personal was intended, and I certainly didn't imply that students
should follow blindly. I simply don't see the present and future of SEM
as bleak in the slightest.

Salaries for microscopists might not be what you or I would like them to
be. If a student of microscopy derives their feelings of self worth from
their salary, then perhaps they shouldn't be looking at microscopy as a
profession. On the other hand, the majority of jobs in microscopy are
with universities and serious corporations. Perhaps salaries are
(arguably) low, but other important benefits are provided.

For now, I'll stick with my opionion that SEM based R&D at the university
level will ultimately expand the possibilities for electron beam
applications in industry, hence creating more opportunities. Go for it
graduate students. You might not want to be a microscopist when you come
out into industry, but the SEM will provide you with a unique view of
what makes things tick. The understanding that goes along with this
vantage point is invaluable to potential employers.

Regards and Good Luck to all.
John Best.


I have been following this string with much interest, but have vowed
not to include my opinion; until now. I have been in electron
microscopy since the late '60s, both as a graduate student and
"technician". For the past 12 years I have taught two TEM and one
SEM class per year at the university level, although I am still classified as an EM
Technician. Mr. Folsom is correct in his realistic approach to the
subject. We all would enjoy working in our "ideal" jobs and "ideal"
situations, but those things only exist at Disneyland.

Mr. Best suggests a different occupation if salary is the main
concern of the technician. We have all had the dream of being
involved in the research which will improve the lot of mankind, but
try raising a family on the salary of an EM technician and see how
long it takes for reality to set in.

In closing, dear graduate students, use electron microscopy as it was
intended to be used. It is a high tech tool and should be considered
a means to an end, and not the end itself.

Franklin Bailey




From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Tue, 6 Aug 1996 15:04:32 -0700
Subject: Durst S-45 Enlarger available

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A friend of mine has a Durst Laborator S-45 enlarger available for sale.
The package includes 4 condenser lenses and 5 Schneider Componon lenses of
various focal lengths, as well as point light source.

Please contact me directly for further details. Serious inquiries only.

John
chandler-at-lamar.ColoState.EDU






From: Nancy K.R. Smith :      smithn-at-uthscsa.edu
Date: Tue, 06 Aug 1996 16:54:15 -0500 (CDT)
Subject: Tracor NS-880 parts

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Microscopy list:
Does anyone happen to have a cassette tape transport for a
Tracor-Northern NS-880 x-ray analysis system (vintage 1975-1979)?
If you do, or know of anyone who does, please contact me directly at
smithn-at-uthscsa.edu.

Thanks





From: schooley-at-mcn.org (Caroline Schooley)
Date: Tue, 6 Aug 1996 14:20:12 -0700
Subject: Your "Ask-a-microscopist" query

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Terri -
I think that you'll find the size information that you want at:


http://www.uq.oz.au/nanoworld/images_1.html
Although the images don't have scale bars, the image index gives
enough info to calculate them, and the micrographs themselves are
excellent.


Caroline Schooley
Educational Outreach Coordinator, Microscopy Society of America
Box 117
Caspar, CA 95420
Phone/FAX (707)964-9460
Email schooley-at-mcn.org






From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 6 Aug 1996 16:14:46 -0500
Subject: Enlarger_advice?

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Message-ID: {n1372774121.91693-at-msmail.tmc.tulane.edu}

Please respond within the next 30 minutes on source and specs for the latest
4x5 enlarger (Omega, Besseler, etc.) Thanks
*********************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} ----} Prof. Pathology & Otolaryngology *
*http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html*
*********************************************************************




From: kathryn.jones-at-stonebow.otago.ac.nz (Kathryn R. Jones)
Date: Wed, 7 Aug 1996 16:43:57 +1200
Subject: Precipitation and evaporation of fluoronanogold label

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Hi,

I have been using fluoronanogold. I aliquoted out the first 0.2ml I
received into eppendorf tubes and cryotubes and placed them in a 4 degrees
celcius fridge (10 or 20=B5l aliquots). A number of the aliquots have
precipitated or completely evaporated or ended up on the lids of the tubes.
I have checked that the temperature of the fridge remains constant.

Has anyone got any suggestions for the storage of this antibody?

Thanks for your assistance
Kathryn






From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 6 Aug 1996 19:14:34 -1000 (HST)
Subject: New SEM images to amuse y'all

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Aloha, Microscopists-

I'm looking forward to seeing many of you next week at the MSA meeting! I
felt guilty for not preparing a poster this year (the data weren't
cooperating), so I spent some time putting together a web page, instead.
These images are a product of the imaginations of a recently re-met old
high-school friend and myself; an eccentric artist and a mad scientist
(='MicroAngelo'). We hope to come up with some more fun stuff soon
('SEMantics'). Check it out at
http://www.pbrc.hawaii.edu/bemf/microangelo.html

See you in Minneapolis!

Tina

***************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu *
***************************************************************************
http://www.pbrc.hawaii.edu/bemf/microangelo.html





From: koster-at-biochem.mpg.de (Bram Koster)
Date: Wed, 7 Aug 1996 09:24:41 +0200
Subject: recording moving sceneries from a Display

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X-Sender: koster-at-spinne.biochem.mpg.de
Message-Id: {v01530500ae2df141b66c-at-[141.61.5.39]}
Mime-Version: 1.0
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Dear microscopists

I am looking for a system capable of recording moving sceneries shown on a
Silicon Graphics workstation display.

My impression is that there are two basic approaches:
(1) based on recording the sequence of displayed images over an
anolog/digital/anaolog converter to a S-VHS videorecorder, and
(2) by burning the digital results after various file-conversions into
a CD-ROM.

For practical purposes the S-VHS approach seems to be more
practical/faster, although the CD-ROM approach in combination with an
interactive CD-player seems to have the future.....

Suggestions on the pros and cons of possibles approaches/systems are very
welcome.




-------------------------------------------
Dr. Abraham J. Koster
Max-Planck-Institute for Biochemistry
Department for Structural Biology
Am Klopferspitz 18A, D-82152 Martinsried
Germany

voice : +49-89-8578 2632
fax : +49-89-8578 2641
email : koster-at-biochem.mpg.de
www : http://www/baumeister/koster.html
-------------------------------------------






From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 7 Aug 1996 09:19:06 -0500
Subject: EnlargerGothanks

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I found out that replacing the two Besseler enlarger we now have with current
models (except Durst which I do not want) would not add any more
functionality, specially for the price of newer models. Thanks to those who
responded so quickly. I was given 45 minutes to make a decision on $$$$.




From: Robert Derby :      derby-at-pb.net
Date: Wed, 7 Aug 1996 11:42:38 -0500
Subject: Fluorgold

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DO NOT FREEZE this material. Just store it in the ffrig. 4 degress is fine.
I have used this, provided by Nanaprobes, it works fine, but just like any
gold labeled material do not freeze, it knocks the gold off, hence your percip.

R.J. Derby





From: schooley-at-mcn.org (Caroline Schooley)
Date: Wed, 7 Aug 1996 17:25:05 -0700
Subject: Meeting time!

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Message-Id: {199608080013.RAA29981-at-mail.mcn.org}
X-Sender: schooley-at-mail.mcn.org
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Content-Type: text/plain; charset="us-ascii"

Unsubscribe.

Caroline Schooley
Educational Outreach Coordinator, Microscopy Society of America
Box 117
Caspar, CA 95420
Phone/FAX (707)964-9460
Email schooley-at-mcn.org






From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Wed, 7 Aug 1996 14:21:10 -1000 (HST)
Subject: LM: Sectioning non-decalcified bone

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Aloha, Microscopists,

A colleague called this morning with questions regarding sectioning
non-decalcified rat bone for LM studies. I know I've seen this subject
pass through here before, but ignored it because I didn't have to do
it...! She has papers that recommend embedding in methacrylate and using
a Jung-K sliding microtome with a HK-2 profile tungsten carbide tipped
knife. We have not yet been able to locate such a beast here on Oahu, and
so we are wondering if a "regular" rotary microtome with a good knife has
a chance of doing the job. Do any of you have any recommendations? Will
a hardened steel knife do it, or should it, indeed, be a carbide blade?
Do rotary microtomes have enough oomph? We are trying to use instruments
at hand, as driving over to the next state is not an option...

Thanks in advance, and see you at MSA!
Tina

http://www.pbrc.hawaii.edu/bemf/microangelo.html

***************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu *
***************************************************************************





From: geoffa-at-amsg.austmus.gov.au (GeoffA)
Date: Wed, 7 Aug 1996 16:43:13 +1100
Subject: MicroLumina: comments?

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Mime-Version: 1.0


Hi to all on the listserver from sunny Sydney,

Any users of the MicroLumina digital camera from Leaf systems willing to
share their impressions/opinions?



::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
Geoff Avern
Manager
Microscopy Laboratories
Australian Museum Email: geoffa-at-amsg.austmus.oz.au
6 College St Ph: (61)(2) 9320 6198
Sydney, Australia. 2000 Fax: (61)(2) 9320 6059
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::








From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Thu, 8 Aug 1996 03:09:55 -0500
Subject: Proceedings of Microscopy & Microanalysis-96 is Now On-Line

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Message-Id: {199608080804.DAA01189-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
wgunning-at-magnum.mco.edu, opmills-at-mtu.edu, jjerome-at-bgsm.edu,
ron-anderson-at-VNET.IBM.COM, rgronsky-at-garnet.berkeley.edu,
lemaster-at-med.unc.edu, bhgf-at-med.unc.edu, Ak5d+-at-andrew.cmu.edu,
rma-at-ahabs.wisc.edu, allanwojtasp-at-em.agr.ca, fulch001-at-maroon.tc.umn.edu,
hainfeld-at-genome1.bio.bnl.gov, spector-at-cshl.org, mvp2-at-cornell.edu,
mueller-at-em.biol.ethz.ch, jjerome-at-isnet.is.bgsm.edu, chestnut-at-pg.com,
perovic-at-ecf.toronto.edu, das3-at-lehigh.edu, mibuckett-at-mmm.com,
vinod.berry-at-gep.ge.com, carter-at-cems.umn.edu, joy-at-utkvx.utk.edu,
jpawley-at-macc.wisc.edu, bentleyj-at-ornl.gov, bondm-at-cesmtp.ccf.org,
fpo-at-oci.utoronto.ca, george-at-igel.mbg.uoguelph.ca, sgmcakay-at-mmm.com,
sbryan-at-phi.com, small-at-gapnet.nist.gov, ian-at-wang.ms.ornl.gov,
magold-at-bcm.tmc.edu, imusselm-at-utdallas.edu, prussell-at-ncsu.edu,
Gilles.L'esperance-at-mailsrv.polymtl.ca, carmen-at-wadsworth.org,
jfmjfm-at-umich.edu, stuartm-at-maroon.tc.umn.edu, Zaluzec-at-AAEM.AMC.ANL.GOV,
ruth.dimlich-at-uccc.san.uc.edu, jrmicha-at-sahp356.sandia.gov,
corbett-at-physics.watstar.uwaterloo.ca, ruth.dimlich-at-uccc.san.uc.edu,
efosten-at-mmm.com, stan-at-lenti.med.umn.edu,
MSABusinessOffice-at-Sparc5.Microscopy.Com, hallel-at-macgw1.crd.ge.COM,
hallel-at-macgw1.crd.ge.COM, corbett-at-physics.watstar.uwaterloo.ca,
jrmicha-at-sandia.GOV, Ruth.Dimlich-at-UC.Edu, alexanderkb-at-ornl.gov,
jonm-at-noran.com, BusinessOffice-at-Sparc5.Microscopy.Com, efosten-at-mmm.com,
carter-at-cems.umn.edu, zaluzec-at-Sparc5.Microscopy.Com,
BusinessOffice-at-Sparc5.Microscopy.Com

Colleagues....

As most of you all know the annual meeting of Microscopy & Microanalysis
will convenes next week in Minneapolis Minnesota (Aug 11-15). For those of you
who will not be able to attend or are still hesitating because your not sure
that a particuliar symposium or set of papers is of interest to you, let me try
to peak your interest on last time. I have arranged for a Low Resolution
copy of every abstract of the Meeting to be available On-Line at the
Microscopy Society of America WWW Home Page effective immediately.
The URL to point your WWW browser to is:

http://www.msa.microscopy.com

The purpose of these on-line access is to provide a simple rapid means of
reference to the majority of information in the proceedings and will be
presented as either a platform or poster. This on-line
access is not meant to replace, but rather to supplement, the Proceedings
of the meeting. This is particuliarly important for those
microscopists/microanalysts
who do not have immediate access to their own or library copies or for
the majority of our international colleagues who cannot travel to the
meeting at this time.

You may login to the MSA WWW site, and search for papers by author,
symosium topic, and/or keywords in the Abstract titles and then view these
abstracts on-line. All abstracts were digitized (from photocopies
not originals... sorry), OCR'ed and then converted into the Adobe
Portable Document Format (PDF). Hot links are provided on the
appropriate page so that you may download a FREE copy of the
public domain PDF reader program for your flavor of computer.

Please note that these documents are all readable, however there are
most definitely OCR errors with most equations superscripts etc... There
has been NO attempt to editing of the PDF documents to correct those
problems at this time, it is a project to undertake sometime in the future.
The quality of the photocopies generally dictated the quality of the
OCR software to recognize text. So some manuscripts will have
more errors than others. The same holds true for the micrographs.

You will of course be able to procure excellent high resolution, versions of
each manuscript by attending the meeting and getting your copy
of the proceedings with your registration (there is still time folks
you can obviously register on-site)! Alternatively you may order a copy
of the proceedings from the Society Business office or our
publisher. Information on all of these options is also available
on the MSA WWW site.

If your not a member of the society, why not take a moment while
your visiting our page and fill out our on-line electronic membership
form. It fast , easy and inexpensive! You'll become a member of MSA
and receive copies of the Journal and reap a variety of valuable benefits
when you become an MSA member.

Hope to see you in Minneapolis, but if you can't be there don't forget
to check the MSA Home Page for daily updates on the meeting. We're
planning to attempt some novel ways for you to be involved with
the meeting even if you cannot be there...

Cheers..... Nestor

Your Friendly Neighborhood SysOp
&
Microscopy & Microanalysis Program Chair








From: Hans-Martin Vaihinger :      Hans-Martin.Vaihinger-at-rz.ruhr-uni-bochum.de
Date: Thu, 8 Aug 1996 09:40:46 +0000
Subject: LR White summary

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Comments: Authenticated sender is {vaihihbt-at-mailhost.rz.ruhr-uni-bochum.de}

Thanks everybody for contributing to the LR White diskussion.

Here is the initial request:
* We are only just beginning to use LR White as embedding medium for
* use in EM- immunocytochemistry. A manual by AGAR SCIENTIFIC LTD.
* distributed through PLANO recommends to bring the specimen from
* 70 % EtOH into LR White/70 % EtOH (2:1) before embedding in pure LR
* White. We find that this mixture very quickly separates into two
* phases. How shall we handle this?
* Furthermore the specimen seems to be cured insuffiently even after
* 24 hours of polymerisation at 55 C. Any comments on this?

... and this is what I received:

I am having exactly the same experience with LR White and
would appreciate seeing the replies. (I have started going to 95%
ethanol in order to get good mixing). (Karen Zaruba)

I would like to add another 'complaint' to the list. Plant tissues
embedded in LR White show signs of severe plasmolysis. I have
broken down the infiltration into 25, 50, 75 and 100% resin/75%
EtOH and prolonged the infiltration stages (+- 2 hrs each) but to
no avail. By the way, the mixture tends to separate as well!
Maybe this is the reason for the shrinkage...? (James Wesley-
Smith)

I've used (or rather TRIED to use) LR White for LM but with
very little success. I found that the blocks were brittle and didn't
stain well with LM stains. Needless to say, I'm not impressed and
have stuck to GMA embedding. I understand that the shelf-life
for LR White is very short; perhaps that was part of my problem
as well as part of yours??? (Delilah W. Irving)

I routinely embed in LRWhite; I always go into 100% EtOH,
then some EtOH:LRW mixture (usually 2:1 and then 1:1) before
straight resin - I don't quite trust the claim that no further
dehydration/infiltration is required. As for curing - we cure at
60C for 36-48 hours; sometimes the surface is still "tacky" at this
point, but I embed such that the sample is always away from any
possible contact with air, so I can just cut the tacky part away.
What kind of molds are you using? Agar capsules, filled and
capped, are great for "chunk" samples; we use the old JB-4
molds for cells on coverslips. (Tamara Howard)

We have used LR WHite frequently and just do everythihng the
same as if we were using Spurr's resin (use the same dehydration
schedule, etc.). THE one thing that we noted that made a big
difference in our hands is to polymerize in fully filled gelatin
capsules (predried in an oven) and use a temperature controlled
block rather than an oven. It seems that our oven did not keep a
very close control on temperature. Change over to using a temp
block (like one commonly used for restriction digests) solved the
problem with poor polymerization. For immunoEM, we
polymerize at 50C, but it takes several days(Robin Wright).

The separation is due to the sample not being actually in 70%
EtOH when it enters the EtOH:LR White mixture. Remember
that the specimen will exchange some water in the 70% step
making the final concentration below 70%. We routinely add an
80% EtOH step and have encountered no problems with
miscibility. Also the 1:2 mixture is made with 80% EtOH.
Insufficient curing could be alleviated by letting the blocks
polymerize for another 24h. But if the LR White is older than the
printed expiration date on the bottle then that may be the cause
too. (Larry Oakford)

You know that LR White needs to be cured in a anarobic
atmosphere? right? Any O2 messes it up,` turns to jelly. And in
my hands a 70% mixture was misable, I got mine from EMS in
Penn. USA. I infiltrated on a rocking table that should keep it
"mixed". But in my hands I always dehydrated completly first
thru 100% ETOH. then into LR White, first change was short
like 1-3 hours then the next was overnight, then cured. I did
years of LR white embeding and labeling if any questions, if you
think I am help E-mail me at derby-at-pb.net. (Robert J. Derby)

I have been using LR White for some time now and my protocol
is as follows. 25% ETOH, 50% ETOH, 70% ETOH, 2x 100%
ETOH, 1:1 LR White to 100% ETOH, pure resin. I usually mix
1 ml resin with 1 ml 100% ETOH by using a Pastuer pipet many
times slowly until the swirling effect goes away and it is a
consistent solution. I then cure for 24 hr at 60 degrees. I have
been doing IC with these sections for 6 months and have not had
too many problems except some post stain garbage. (Christine
Ann Brantner)

For IC of plant tissue, we bring the tissue into 100% EtOH, then
1:3 100% LRWhite: 100% EtOH, then 1:1, then 3:1 then 100%
LRWhite. We then change the 100% LRWhite 2-3 times during
about 24h infiltration. In other words, we treat it pretty much
like Spurr's. You can use acetone or methanol as the dehydrating
agent too. I've heard you can leave some water or ethanol in the
specimen and still get good embedding, but it's never worked for
us - causes soft blocks. We found overnight polymerisation
usually sufficient as long as the specimen is well infiltrated and
air is excluded. (Rosemary White)

Use 100% alcohol to make your 70%. You may have a more
dilute concentration of alcohol than you think. It happened to us.
(Rosemary Walsh)

We just bring the specimen from 30--} 50--} 70--} 96--} 100%
EtOH into LR White, 3X1 hour, before embedding in pure LR
White. 48 hours of polymerisation at 60 C. (Gary Chinga)

The 70% EtOH needs to be made up freshly just before use.
Even stored in a tightly stoppered bottle, the concentration of the
EtOH seems to decrease. This solved my similar problems. 70%
EtOH seems to be the bare mininum that LR White will mix
with; as soon as the concentration drops below this there are
problems with infiltration, curing and the final blocks are brittle.
I
keep the LR White in the fridge and use it without warming. All
vials are rotated continuously during processing. I embed the
tissue in gelatin capsules in a normal embedding oven. I rarely
have problems with the blocks, though I must admit I would only
use LR White for immuno, never as a replacement for resin.
(Diana van Driel)

When using LR White, we dehydrate specimens by passing
through a 70, 85, 95 and 100% EtOH serie. After doing this we
bring the specimens in EtOH 100% / LR White (1:1) for 60 min
before pure resin. The fact that the resin seems to be cured
insuffiently does not surprise me at all : I have the same problem,
I am working on plant cells and the cell wall does not adhere well
to the resin. I am still trying to solve this last point... Good
luck (Pascal Veys)

You can use DMF(dimethyl formamide) instead of the ethanol
when dehydrating. LR white must be fairly air tight when it is
polymerized. The oxygen from the air is enough to cause this
problem of poor polymerization. Are you using gelatin caps?
Also I gently bubble nitrogen through the LR white to
remove any oxygen, then I use it in the final embedding. (Patricia
Zerfas)

We have used LR White routinely for a number of years. We find
the best results are obtained by dehydrating to 100% Ethanol.
The main things to watch out for are keeping the temperature
well controlled and excluding oxygen (which can penetrate many
commonly used moulds such as BEEM capsules). Excessive
brittleness seems to be caused by the embedding temperature
being too high (perhaps only a degree or two above 60C). Fresh
LR White has a shelf life of 1 year at 4C though we have used
older batches occasionally. The major problem with old batches
can be premature polymerising. This is not a great problem if it
occurs in the bottle but more so if it occurs whilst infiltrating the

sample. Uncatalysed resin, which comes with a powder catalyst,
has an almost indefinite shelf life and we make up a fresh bottle
of active resin just before the old one runs out. (Ian Hallett)

We have used LR White for over 15 years. The biggest problem
that we have is when there is EtOH left in the material before it
goes to 100% LRWhite. To take care of that problem we do
NOT put a mixture of alcohol and LR Whita before going to
pure. We definitely do go to 100%, then 3 changes of pure LR
White, then into capsules. The gelatinous mess that results from
the EtOH/LR White was never found to polymerize even after 2
yrs., even after try ing to dry it out and all sorts of other
manipulations to save a valuable sample. (Judy Murphy)

To the folks discussing embedding in LR White. I routinely
embed material in Lrwhite for use in immunocytochemistry
experiments. Some solutions to the polymerization problem are
to simply extend the polymerization time at 55 degrees or to use
the room-temperature cure method by which a catalyst is added
to the resin and it cures in about ten minutes or so. I am currently
comparing room-temperature and 55 degree cure blocks of the
same material (Clarkia stigmas). I have taken 160 serial thick (1
um) sections from each block and see no difference between the
blocks in terms of sectioning. For my infiltration, I dehydrate to
100% ethanol with three changes for 15 minutes each. Then I go
to a 1/3 LRWHITE:2/3 ETOH mix for 24 hrs, a 2/3
LRWHITE:1/3 ETOH for 24 hrs, a 24 hr 100% LRWHITE, and
then a final 6 hr period of infiltration with 100% LRWHITE. For
the ETOH/Resin mixes I draw the solution up into the pipette
and dispense it back into the vial a few times to mix it initially,
for all the infiltration steps I leave the vials on a rotator so that
they are constantly being mixed. I have never had problems with
infiltration or separation of the resin and solvent. (Michael A. Fremarek)

I routinely used LR White a few years ago for EM
immunocytochemistry. It was definately easier to go through
95% EtOH and then into LR White. The most consistent curing
was at 50 C under nitrogen. I would evacuate a vacuum oven
and then flood the chamber with nitrogen to exclude any oxygen.
(Paulette Brunner)

I have done extensive immunochemistry with LRW hard grade
using cell monolayers. I always infiltrate to 100% ETOH with a
freshly opened bottle. I do a 50:50 LRW : ETOH for a minimum
of three hours or overnight, then go to 100% LRW. I have found
a 55 oven to be inadequate. I use 58 - 60 oven for two days. If
you are using cell cultures permanox solvent resistant petri dishes
can be used for the entire procedure, filling to the top and using
another bottom to create an anerobic seal. Then sawing your
sample out and remounting it. The best immunochemistry text I
have found is by Griffiths "Fine Structure Immunocytochemistry"
(Shelley Landon Kaurin)

**************************************************************
Hans-Martin Vaihinger
Ruhr-University of Bochum
Comparative Endocrinology Research Section
Building ND 5/37
44780 Bochum
GERMANY
*********************************************************
phone ++49 234 700 4329
fax ++49 234 709 4551
email Hans-Martin.Vaihinger-at-RZ.Ruhr-Uni-Bochum.de





From: tsr-at-codon.nih.gov (tsr)
Date: Thu, 8 Aug 1996 12:10:43 -0400
Subject: Balzers part needed

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We needs parts for, or a complete DPA 101 Vacuum control unit for a Balzers
301 freeze etch unit (sale or barter). Also need crystals for QCM (old
style). Could be interested in a complete 301unit. Please reply
privately to: Tom Reese. } treese-at-mbl.edu {

...Thanks....Tom






From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 08 Aug 96 13:37:53 EDT
Subject: Re: LM: Sectioning non-decalcified bone

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Tina:

While I don't have an answer for you, I would suggest that you contact Don
Kimmel at Creighton Univ. Unfortunately, I don't have his e-mail - I did call,
but he was not in. Anyway, you can reach him at: TEL: 402-280-4470 or FAX:
402-280-5173.

As I recall, he was sectioning rat mandibles that he encapsulated in methyl
methacrylate. He sectioned them into 100u slices using our Low Speed Diamond
Wheel Saw. I think you could get the slices thinner using our sample rotation
attachment for the saw. I know Don was considering this at one point, but I'm
not certain if he has pursued it. Anyway, he would be worth talking to. I hope
this helps.

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
http://www.southbaytech.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.





From: NORM OLSON :      NHO-at-bragg.bio.purdue.edu
Date: Thu, 8 Aug 1996 14:06:04 -0500 (EST)
Subject: Cryoelectron Microscopy Workshop

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Purdue University is offering an intensive, four-day workshop
in the basics of cryo-electron microscopy. The course will run
from November 3 through November 6 and will include theoretical
discussions and hands-on demonstrations in preparing
vitrified samples of biological macromolecules. Students will also learn
low-dose, phase-contrast imaging procedures and image recording on
both film and CCD cameras. An introduction to the methods of
image analysis and three-dimensional image reconstruction will
also be given. For registration information contact: Susan Umberger,
7136-U, Purdue University, Division of Conferences,
1586 Stewart Center, Room 116, West Lafayette, Indiana 47907-1586.
Phone: 317-494-7217. For course content information see the
web site at http://bilbo.bio.purdue.edu/~workshop/ or contact
workshop-at-bilbo.bio.purdue.edu. The workshop fee is $1200 US and
includes course materials, lunches, a banquet and lodging.




From: Murphy, Judy :      murphy-at-sjdccd.cc.ca.us
Date: 8 Aug 1996 15:13:49 -0800
Subject: no registration stuff

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Message-ID: {n1372605202.28518-at-sjdccd.cc.ca.us}

I am leaving for Minneapolis in a few moments and have not received my
tickets, registration badge and all the stuff that was apparently supposed to
be sent out. Please regenerate them so I can pick them up at registration.
Thanks,
Judy Murphy

Judy Murphy, PhD
Microscopy Technology Center
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5600
e-mail: murphy-at-sjdccd.cc.ca.us
program web page: http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html





From: h.liang-at-unsw.edu.au (Helena Liang)
Date: Fri, 9 Aug 1996 16:50:22 +1000 (EST)
Subject: "NO SUBJECT"

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PLEASE SUBSCRIBE
Thank you
Helena





From: Probing & Structure :      pns-at-ultra.net.au
Date: Fri, 9 Aug 1996 21:41:18 +1000
Subject: Please pass on this information to your colleagues.

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Take care
Jim Darley

Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site http://www.ultra.net.au/~pns/
***********************





From: versaci raul :      versaci-at-cnea.edu.ar
Date: Fri, 09 Aug 1996 07:06:55 -0700
Subject: JEOL, why?

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Message-ID: {320B45FF.5445-at-cnea.edu.ar}

Since our bidding for a transmission analytical electron microscope was
legally observed by the local JEOL representative, we would like to know
if such situation has ocurred in other countries. Thank you.
R. A. Versaci
Comision Nacional de Energia Atomica
Departamento de Materiales
Buenos Aires-Argentina
versaci-at-cnea.edu.ar




From: Michael J. Lyon :      lyonm-at-vax.cs.hscsyr.edu
Date: Fri, 09 Aug 1996 09:34:27 -0400
Subject: Fluoro-Gold

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Could anyone help me with a vendor for fluoro-gold. I would like to use
this for retrograde tracing. The only info I have is a company called
Fluorochrome. I don't know where it is located. Any help would be
appreciated.

Thanks




From: Susan Carbyn (Paula Allan-Wojtas) :      AllanWojtasP-at-em.agr.ca
Date: Fri, 09 Aug 1996 10:40:44 -0400
Subject: Courses in Canada?

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Message-Id: {s20b15c9.089-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

I was wondering if there are any EM workshops offered in Canada (in
particular on the East Coast). Workshops of interest are Cryo, Image
Analysis or Insitu hybridization techniques. Please respond to me
directly at carbyns-at-em.agr.ca
Thanks,
Susan





From: changj-at-ecn.purdue.edu (Jessica Chang)
Date: Fri, 9 Aug 1996 10:47:48 -0500 (EST)
Subject: postdoc opening

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Post-doc on TEM available - The NSF-Materials Research Science and Engineering
Center for Technology-Enabling Heterostructure Materials at the
School of Electrical and Computer Engineering of Purdue University has an
opening for a post-doctoral research associate. The position
requires hands-on experience on transmission electron microscopy (TEM)
including electron diffraction pattern analysis, bright-field, dark-field,
weak-beam, and high-resolution TEM, as well as specimen preparation for
plan-view and cross-sectional TEM. The employee will be responsible for
the characterization of molecular-beam epitaxy (MBE) grown II-VI, III-V
and/or nitrides wide bandgap semiconductor heterostructure devices,
including analysis of the failure mechanism of II-VI lasers and light-
emitting diodes. Duties will also include maintenance of sample
preparation facilities including a Gatan Dual ion mill and a Jeol-2000 EX
high-resolution transmission electron microscope which is under service
contract. Rate of pay will be commensurate with qualifications and
experience. All applications should send resumes to Professor Jessica C.P.
Chang, School of Electrical and Computer Engineering, Purdue University,
West Lafayette, IN 47906-1285; Fax:317-494-6951; Email:changj-at-ecn.purdue.edu
Purdue University is an AA/EEO/ADA employer.






From: Nancy Desmond :      nld-at-avery.med.virginia.edu
Date: Fri, 9 Aug 1996 13:07:43 -0400
Subject: unsubscribe

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unsubscribe


--
Nancy L Desmond, Ph.D. nld-at-virginia.edu
Department of Neurosurgery 804.924.5607 (voice) 804.982.3829 (fax)
University of Virginia Health Sciences Center, Box 420
Charlottesville, VA 22908




From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Fri, 9 Aug 1996 14:11:36 EST
Subject: Re: JEOL, why?

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} Since our bidding for a transmission analytical electron microscope was
} legally observed by the local JEOL representative, we would like to know
} if such situation has ocurred in other countries. Thank you.


I experienced a similar situation several years ago when we were
purchasing a SEM, and our university (in Louisiana at that time)
requested bids. We had present at the opening of the sealed bids,
representatives from Hitachi, Zeiss, ISI, AMR, and ETEC. JEOL did
not send a representative. One of the reps later told me that they
just occasionally check to see how the competition bids on detailed
specifications. This was my only experience of this nature.



-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Jane A. Fagerland (847) 935-0104 :      FAGERLAND.JANE-at-igate.pprd.abbott.com
Date: Fri, 09 Aug 1996 09:13:00 -0500 (CDT)
Subject: LM: Sectioning undecalcified bone

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Mr-Received: by mta RANDB; Relayed; Fri, 09 Aug 1996 09:25:45 -0500
Mr-Received: by mta MCM$RAND; Relayed; Fri, 09 Aug 1996 09:25:47 -0500
Mr-Received: by mta RANDC; Relayed; Fri, 09 Aug 1996 09:26:04 -0500
Alternate-Recipient: prohibited
Disclose-Recipients: prohibited
Content-Return: prohibited

Tina,

I recently did a small project involving whole undecalcified rat "knee"
joints. I embedded them in JB-4 glycol methacrylate using an
infiltration schedule that took several days. The blocks were sectioned
using a Microm microtome and a tungsten carbide knife. It was possible
to cut sections at 5 microns and thinner. The Microm has a motor-driven
cutting stroke that I think is probably necessary to get through the
bone, but I have never tried anything else so I can't say for sure. I
can give you more details if you'd like to contact me directly.

Jane A. Fagerland, Ph.D.
Abbott Laboratories
Abbott Park IL 60064






From: Woody.N.White-at-mcdermott.com
Date: 9 Aug 96 15:53:00 -0500
Subject: Re: Good Time Hoax

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This virus alert was noted several months ago. The consensus of every
sysop with whom I have communicated (numerous) is that this virus is a
hoax. If ANYONE has experienced it - first hand, please post.

Woody White

woody.n.white-at-mcdermott.com

Babcock & Wilcox Research




From: John.Wheatley-at-asu.edu (John C. Wheatley)
Date: Fri, 09 Aug 1996 11:45:59 -0700
Subject: Re: Good Time Hoax

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} X-Sender: crozier-at-csss2.la.asu.edu
} Mime-Version: 1.0
} Date: Thu, 8 Aug 1996 09:53:46 -0700
} To: wheatley-at-csss.la.asu.edu
} From: Crozier-at-ASU.Edu (Peter Crozier)
}
} John,
}
} Can you please post this to the microscopy list server
}
} Thanks
}
} Peter
}
} Dear Colleague,
}
} Please bring this position to the attention of any qualified candidates.
} Thanks for your assistance.
}
}
} Post-Doctoral Research Associate
}
}
}
} A post-doctoral research associate position is available with the
} Industrial Associates Program in Transmission Electron Microscopy at the
} Center for High Resolution Electron Microscopy at Arizona State University.
} The Industrial Associates Program consists of member companies with an
} interest in advanced transmission electron microscopy. The successful
} candidate will gain experience working on real industrial materials
} problems in an academic setting. This unique perspective provides
} excellent training for individuals interested in expanding and broadening
} their skills.
}
} The position offers opportunities to work on a wide range of
} technologically significant micro-characterization problems using the most
} advanced state-of-the-art transmission electron microscopy techniques. In
} addition to applying existing microscopy techniques to advanced materials
} (especially heterogeneous catalysts and electronic materials), the
} Industrial Associates Program also develops and evaluates new TEM
} techniques for nanocharacterization. Areas of particular interest include
} chemical imaging by HREM, annular dark-field imaging and electron
} energy-loss techniques, electron crystallography of zeolites, and in-situ
} environmental microscopy.
}
} The successful candidate should have a Ph.D. in physical sciences, with
} extensive experience in analytical microscopy, HREM imaging and STEM
} techniques. Experience in the areas of semiconductors and heterogeneous
} catalysts is preferred. An ability to interact well with others and assist
} industrial scientists in materials problem solving is essential. The
} initial appointment will be for a period of one year with renewal
} conditional on future funding.
}
} Applicants should submit their resume together and the names of 3 referees to:
}
} Dr. Peter A. Crozier
} Industrial Associates Program
} Center for Solid State Science
} Arizona State University
} Tempe, AZ 85287-1704
} Email:Crozier-at-asu.edu
}
} Peter A. Crozier, PhD (602) 965-2934
} FAX (602) 965-9004
} Crozier-at-ASU.edu
} ASU- Industrial Associates Program
} WWW URL: http://www.asu.edu/clas/csss/IAP
}

John C. Wheatley
Lab Manager
Center for High Resolution Electron Microscopy
BOX 871704
Tempe, AZ 85287-1704
Phone: (602) 965-3831
FAX: (602) 965-9004
John.Wheatley-at-ASU.Edu






From: masur-at-Inka.MSSM.EDU (Dr. Sandra Masur)
Date: Fri, 9 Aug 1996 13:52:17 -0400 (EDT)
Subject: Announcment NYSEM symposium, September 20, 1996 ADHESION MOLECULES AND

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EXTRACELLULAR MATRIX

NEW YORK SOCIETY OF EXPERIMENTAL MICROSCOPISTS
PRESIDENTIAL SYMPOSIUM
& MOUNT SINAI SCHOOL OF MEDICINE, CUNY
present
September 20, 1996, 9 a.m. to 5 p.m.

STOP AND GO:
ADHESION MOLECULES AND EXTRACELLULAR MATRIX

Overview
Dr. David COLMAN, Past-President NYSEM
Mount Sinai School of Medicine, NYC

EXTRACELLULAR MATRIX
Dr. Jean SCHWARZBAUER, Princeton University, NJ

Regulation of Fibronectin Assembly and Matrix Function

Dr. David BIRK, Tufts University, Boston, MA
Regulation of Collagen and Matrix Assembly


INTEGRINS: MATRIX-CELL AND CELL-CELL INTERACTION
Dr. Filippo GIANCOTTI, New York University School of Medicine, NY
Integrin Mediated Adhesion and Signaling

Dr. Judith M. WHITE, University of Virginia Health Sciences,Charlotesvile VA
Integrins and Disintegrins in Fertilization and Development


CELL-CELL JUNCTIONS
Dr. Barry GUMBINER, Memorial Sloan-Kettering Cancer Center, NY
Adherens Junctions

Dr. David SPRAY, Albert Einstein College of Medicine, Yeshiva University,
the Bronx, NY
Gap junctions: What We've Learned About Their Function from
Connexin Knockout Mice


CELL MIGRATION
Dr. Denise MONTELL, Johns Hopkins University, Baltimore, MD
Regulation of Cell Migration During Drosophila Development.


Dr. Frederick MAXFIELD, Cornell University Medical College, NY
Calcium Regulation of Adhesion and Motility

FRIDAY SEPT 20, 1996, 9 AM to 5:00 PM

The Stern Auditorium
The Mount Sinai School of Medicine
Madison Avenue and 100th Street
New York City

For information:
Dr. Marie Filbin, NYSEM secretary
e-mail: filbin-at-genectr.hunter.cuny.edu
phone: 212-772-5270
OR
Dr. Sandra K. Masur, NYSEM President
e-mail: masur-at-msvax.mssm.edu
phone: 212-241-0089

NYSEM Symposium and Reception Pre-Registration Fees (post-marked by Sept 16)

$12.00 Pre-registration for NYSEM members
$16.00 Pre-registration for non-members
$ 5.00 Pre-registration for students

Registration at door:
$20.00 for NYSEM members and non-members, $ 8.00 Registration for students


NAME:______________________________________________________

AFFILIATION:________________________________________________

_____________________________________________________________

_____________________________________________________________

AMOUNT ENCLOSED_____________

PLEASE SEND REGISTRATION FEE AND FORM before Sept 16 TO:

Dr. Marie Filbin
Secretary, NYSEM
Hunter College
Department of Biological Sciences
695 Park Avenue
New York, NY 10021

Best regards,

Sandra K. Masur, Ph.D. masur-at-inka.mssm.edu
Box 1183
Depts of Ophthalmology,and
Cell Biology/Anatomy
Mount Sinai School of Medicine phone: 212-241-0089 or 6544
New York NY 10029-6574 fax: 212-289-5945






From: Kathy Walters :      kwalters-at-emiris.iaf.uiowa.edu
Date: Fri, 9 Aug 1996 16:22:54 -0500 (CDT)
Subject: Iowa Microscopy Society meeting

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The Iowa Microscopy Society (IMS) will hold it's annual meeting on
September 26 and 27, 1996 in Iowa City. This year we will combine our meeting
with an open house of the Central Microscopy Research Facility. The
seminars will take place on the University of Iowa campus in the Eckstein
Medical Research Building.

September 26th, Thursday

7:30-9:00am Registration, poster and exhibiter setup

8:00 am Welcoming Remarks by Richard Sjolund-President,IMS

8:20 am "Imaging Muscle: From 3-D to 2-D and back again"
By Margaret Goldstein, Baylor College of Medicine

9:10 am "Resinless Sections for Transmission and Scanning
Electron Microscopy"
By Marek Malecki, University of Wisconsin, IMR

10:00 am BREAK: Refreshments, posters, exibitors

10:20 "Microwave Technology: Application of Clinical
Medicine and Research"
Rick Giberson, Ted Pella Inc.

11:10 "An Improved Procedure or Alternate Approach to
Cryo fixation for Both Light and Electron Microscopy"
Fred Lightfoot, George Washington University

12:00 IMS Business Meeting

12:15 Lunch Break

1:40 pm "Visualization of Oral Drug Delivery"
Christopher Squier, University of Iowa

2:10 pm "Immunological Approach to the Study of Phloem"
Richard Sjolund, University of Iowa

2:40pm "Fluorescence Banding in Stalagmites"
Luis Gonzalez, University of Iowa

3:10pm BREAK: Refreshments, posters, and exhibitors

3:40pm "In-Situ Hybridization Techniques"
Rebecca Reiter, University of Iowa

4:00pm "Human Pathogenic Bacteria: Elucidation of
Pathogenic Mechanisms by Microscopy"
Michael Apicella, University of Iowa

4:30pm "Cell Interactions in the Tomato Root Meristem"
Eugene Syzmkowiak, University of Iowa

5:00pm RECEPTION, award presentations.

September 27th, Friday Workshops

"Microwave Technology: Application to Clinical Medicine and Research"
Rick Giberson, Ted Pella Inc.

"Resinless Sections for SEM and TEM"
Mereck Malecki, University of Wisconsin, IMR

"Immunohistochemical and Immunofluorescence Techniques Using
Monoclonal Antibodies"
Richard Sjolund, University of Iowa

"Variable Pressure SEM-Hitachi S2460N"
CMRF open house

"Bio-Rad MRC-1024 Laser Scanning Microscope"
CMRF open house

Staff and Student poster competion for cash prizes. Abstracts due
September 10.

Conference Preregistraion: Meeting day Registration:

Regular-$8.00 Regular-$12.00
Student-$6.00 Student-$8.00

Workshop fees are $15.00 each except for open house, which is free.

A box lunch will be available on the 26th for $5.00 if purchased in advance.

Contact Kathy Walters, IMS secretary
for further information:


Kathy Walters / /
Research Assistant III / /\
Center for Microscopy Research / /\ \
University of Iowa /_/ \ \
85 EMRB ____ ((O))
Iowa City, Iowa 52242 | | / /
|| / /
email: kwalters-at-emiris.iaf.uiowa.edu -----------
fax: (319)335-8049 -------------
www: http://www.uiowa.edu/~cemrf






From: Probing & Structure
Date: 8/9/96 9:37 AM
Subject: Please pass on this information to your colleagues.

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {n1372521588.78537-at-macmail7.lbl.gov}
"Probing & Structure" {pns-at-ultra.net.au}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Reply to: RE} Computer virus alert -email!

Apparently this hoax was brought to you by an AOL user.
For more info see --

http://www.lbl.gov/ICSD/Security/good-times-hoax.html

or

ftp://usit.net/pub/lesjones/good-times-virus-hoax-faq.txt

or

http://ciac.llnl.gov/ciac/virdb/VIRS0360.TXT

or even

http://ciac.llnl.gov/ciac/CIACVirusDatabase.html

Mike
--------------------------------------


Take care
Jim Darley

Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site http://www.ultra.net.au/~pns/
***********************







From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)
Date: Sat, 10 Aug 1996 12:45:26 +1200
Subject: TEM: FNA gelling technique

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X-Sender: st004718-at-brandywine.otago.ac.nz
Message-Id: {v01540b01ae2eaf2f627c-at-[139.80.120.152]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Zygmunt Pocswa asks:

I am trying to find the best method to process fine needle aspirates (FNA)
for diagnostic electron micrsocopy. Most of the FNA specimens contain very
fine cells and are either needle washings or contain a lot of red blood
cells.
I have used the technique of centrifuging the FNA between solution changes
but find with the small amount of material availiable too much loss of
specimen is occuring.
As well, I have recently tried geling the FNA in 8% bovine serum albumin,
however I have found that the BSA is not gelling well.

Does anyone have a reliable method, hopefully using BSA, for processing
fine needle aspirates for diagnostic EM?
This may also include processing FNA material on a Giemsa or PAP stained
slide for EM.

Thanks in advance.

Please reply to richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood)


Richard Easingwood
South Campus Electron Microscope Unit
School of Medical Sciences
University of Otago
PO Box 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254
e-mail: richard.easingwood-at-stonebow.otago.ac.nz








From: em-at-mediacity.com (Ed Monberg)
Date: Fri, 9 Aug 1996 20:14:17 -0700
Subject: why z NOT ?

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Message-Id: {26080914280323-at-vms2.macc.wisc.edu}

} Since our bidding for a transmission analytical electron microscope was
} legally observed by the local JEOL representative, we would like to know
} if such situation has ocurred in other countries. Thank you.
} R. A. Versaci
} Comision Nacional de Energia Atomica
} Departamento de Materiales
} Buenos Aires-Argentina
} versaci-at-cnea.edu.ar

I witness bid openings HERE, at both commercial and
gov awards, and by the way,
south of the (canadian) border is not where ANYONE trusts the honesty of
bureaucrats ! I suggest that you want MORE witnessing, not less - that way
your prices can improve even further, especially if the bidding remains
open !!

Good luck, hope you get better prices, and hoist a cup to JOEL !



Regards,



(signed) Ed Monberg {em-at-mediacity.com}

--------------------------------------------------

510-429-1060 Fax 429-1065
LMDC, (Laser & Motion Development Co.)
3101 Whipple Road
Union City, CA 94587-1216

FOR A TEXT CATALOGUE:
For our Most recent Catalogue of "On Hand" EQUIPMENT:
Send empty mail to: {Cat-at-lasermotion.com}


Our web page: http://www.lasermotion.com (Is beginning to take shape!)
Our e-mail: office-at-lasermotion.com






From: Townsend, Jon :      townsend-at-sjdccd.cc.ca.us
Date: 9 Aug 1996 18:15:39 -0800
Subject: help

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Message-ID: {n1372507785.48020-at-sjdccd.cc.ca.us}

help




From: Townsend, Jon :      townsend-at-sjdccd.cc.ca.us
Date: 9 Aug 1996 18:15:39 -0800
Subject: help

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Message-ID: {n1372507785.48020-at-sjdccd.cc.ca.us}

help




From: W Dale Branton :      branton-at-maroon.tc.umn.edu
Date: Fri, 09 Aug 1996 21:42:10 -0500
Subject: help

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X-Sender: branton-at-maroon.tc.umn.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

list short




W. Dale Branton, Ph.D.
Associate Professor of Physiology
Director of Undergraduate Studies
6-255 Millard Hall
University of Minnesota
435 Delaware St. S.E.
Minneapolis, MN 55455
(612) 625-8977
(612) 625-5149 (FAX)







From: marsh-at-msg.ucsf.edu
Date: Fri, 9 Aug 1996 20:36:06 -0700
Subject: help

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what is imrlist-at-NETCONCEPTS.COM, and why the fuck am I getting a whole
slew of unsolicited e-mails from them? i never signed up onto any listservs
and i want to know who put me on this list and why.
-wall





From: W Dale Branton :      branton-at-maroon.tc.umn.edu
Date: Fri, 09 Aug 1996 21:42:34 -0500
Subject: help

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X-Sender: branton-at-maroon.tc.umn.edu
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

list global




W. Dale Branton, Ph.D.
Associate Professor of Physiology
Director of Undergraduate Studies
6-255 Millard Hall
University of Minnesota
435 Delaware St. S.E.
Minneapolis, MN 55455
(612) 625-8977
(612) 625-5149 (FAX)







From: f44-at-cosmail1.ctd.ornl.gov (Thomas L. Ferrell)
Date: Fri, 9 Aug 1996 23:27:15 -0400
Subject: W Dale Branton

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Message-Id: {v02140b01ae31b1be5742-at-[128.219.165.168]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

list global




W. Dale Branton, Ph.D.
Associate Professor of Physiology
Director of Undergraduate Studies
6-255 Millard Hall
University of Minnesota
435 Delaware St. S.E.
Minneapolis, MN 55455
(612) 625-8977
(612) 625-5149 (FAX)






From: marsh-at-msg.ucsf.edu
Date: Fri, 9 Aug 1996 21:24:38 -0700
Subject: integrated microscopy?

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to those who have been getting mail from imrlist and don't know what it
is about: i have been looking at the affiliations of the people whose
undelivered mail is being bounced back to me, and it seems as though most
ppl on this list are in some way involved in biology or medicine. having
realized this, i made the connection that "IMR" stands for the integrated
microscopy resource at madison wisconsin, and I had got a few announcements
from them recently. have the other people who are getting unsolicted mail
from imrlist also been getting announcements from the integrated microscopy
resource? if so, all we have to do is find out who is in charge over there
and get them to take us off this stupid list.
-wall
p.s. has anyone besides me tried sending mail to imrlist? so far i
have gotten about 50 undeliverable-mail messages bouncing back.




From: ard-at-atom.ansto.gov.au (Arthur Day)
Date: Sat, 10 Aug 1996 17:47:19 +1000
Subject: OK, what sort of list are you??

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Message-Id: {v02140b01ae31eeca85c4-at-[137.157.95.82]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"








From: Amy Aslamkhan :      aslamkha-at-hawaii.edu
Date: Fri, 9 Aug 1996 23:18:17 -1000
Subject: UNSUBSCRIBE

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Message-ID: {320C53D7.4386-at-hawaii.edu}

I do not remember subscribing to this listserver. Please UNSUBSCRIBE
me. I already receive mail from another listserver, and that is quite
enough to keep up with.




From: Stephen Harold Loughborough :      shl1-at-st-andrews.ac.uk
Date: Sat, 10 Aug 1996 13:46:46 +0100 (BST)
Subject: Immunolocalisation...

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Dear all,

I am interested in using a TEM to examine enzyme distribution, As such, I am
wondering if there is an appropriate method for quantification of gold
markers?

cheers,

Stephen Loughborough.
The University of St Andrews,
Harold Mitchell Building,
Greenside Place,
St Andrews,
FIFE,
Scotland.




From: lmeserv-at-bgnet.bgsu.edu (Lee Meserve)
Date: Sat, 10 Aug 1996 07:44:37 -0500
Subject: UNSUBSCRIBE

Contents Retrieved from Microscopy Listserver Archives
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X-Sender: lmeserv-at-mailbox.bgsu.edu
Message-Id: {v01510102ae3234509aa6-at-[129.1.254.149]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I am unsure where you received my address to add to your list, but PLEASE
DELETE IT! Unsubscribe from imrlist.

Lee A. Meserve






From: FRANCISCO J HERNANDEZ BLAZQUEZ fzea - zab 0195 616122 - 283 :      fjhblazq-at-usp.br
Date: Sat, 10 Aug 1996 10:48:23 -0300 (GRNLNDST)
Subject: EM: ultramicrotomes

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I'm looking for a good ultramicrotome,
In Brazil I only know the Leica representatives

Does anyone have experience with Leica ultramicrotomes?
What equipment do you recommend ?

How may I contact the firms who sell ultramicrotomes?
If a company has this equipment for sell, please
send me a message, fax or letter.

I would be very grateful for any answer.
=============================================================================
Francisco Javier Hernandez Blazquez |
Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-spider.usp.br
Zootecnia e Engenharia de Alimentos | Voice: 55 195 616122 r. 265
Departamento de Ciencias Basicas/Histologia| r. 278
Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 195 618606
CEP 13630-000 Pirassununga (Sao Paulo) |
BRAZIL |
==============================================================================







From: XiaoGuang Ning :      ningx-at-mcmail.cis.mcmaster.ca
Date: Sat, 10 Aug 1996 09:31:32 -0400 (EDT)
Subject: sign off, help

Contents Retrieved from Microscopy Listserver Archives
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I want to sign off! Actually, I never signed on.





From: William Wasserman :      wwasser-at-orion.it.luc.edu
Date: Sat, 10 Aug 1996 08:18:53 -0500 (CDT)
Subject: Re: your mail

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unsubscribe

I am receiving listserve email from many people from
imrlist-at-netconcepts.com PLEASE REMOVE ME FROM THIS LISTSERVER!


-------------------------------------------------------
William J. Wasserman | e-mail: wwasser-at-luc.edu
Associate Professor | phone: (312)508-2337
Loyola University | fax: (312)508-3646
Dept. of Biology | http://orion.it.luc.edu/~wwasser
-------------------------------------------------------







From: Sal Pietromonaco (MED_ONC) :      SPIETRO-at-COBRA.UNM.EDU
Date: Sat, 10 Aug 1996 09:01:29 +0000
Subject: list

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UNSUBCRIBE ME !

SAL PIETROMONACO





From: zena-at-radlab.ucsf.edu (Zena Werb)
Date: Sat, 10 Aug 1996 08:46:33 -0700
Subject: unsubscribe

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Message-Id: {v02120d00ae325f00a6b5-at-[128.218.24.14]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

UNSUBSCRIBE

I am unsure where you received my address to add to your list, but PLEASE
DELETE IT! Unsubscribe from imrlist.



Zena Werb
Laboratory of Radiobiology and Environmental Health
University of California, LR-102
3rd and Parnassus Avenues
San Francisco, CA 94143-0750
Tel: (415) 476-4622
Fax: (415) 476-0721
Assistant: (415) 476-1636
Laboratory: (415) 476-4758
Internet: zena-at-radlab.ucsf.edu






From: m_martindale-at-uchicago.edu (mark q. martindale)
Date: Sat, 10 Aug 1996 10:56:59 -0500 (CDT)
Subject: unsubscribe

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PLEASE TAKE ME OFF OF THIS LIST. THANKS.

:) mqm
Department of Organismal Biology and Anatomy
University of Chicago
1027 E. 57th St.
Chicago, IL 60637

(312) 702-9228 (office)
(312) 702-0428 (lab)
(312) 702-0037 (FAX)






From: smithp01-at-MCRCR6.MED.NYU.EDU
Date: Sat, 10 Aug 1996 08:34:32 -0400 (EDT)
Subject: Re: UNSUBSCRIBE

Contents Retrieved from Microscopy Listserver Archives
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On Fri, 9 Aug 1996, Amy Aslamkhan wrote:

} I do not remember subscribing to this listserver. Please UNSUBSCRIBE
} me. I already receive mail from another listserver, and that is quite
} enough to keep up with.

I'm on this list. And I really don't mind that I got added.

However, the polite thing to do for the list owner is to post the
mechansim to unsubscribe. Then people can do it for themselves without
deluging the net (and other subscribers) with demands to be removed that
frankly arn't at all likely to be fulfilled....

+------------- 8F EF 51 4E 4F 23 22 AF 6A 41 D6 C0 AE 31 B1 82 -------------+
|Ross Smith, Research Computing Resource, Department of Cell Biology, NYU-MC|
|E-Mail: SMITHP01-at-MCRCR.MED.NYU.EDU Phone: (212)263-5356: FAX: (212)263-8139|
+-------------- {http://www.med.nyu.edu/people/P.R.Smith.html} --------------+





From: Schoonhoven, Robert :      rschoonh.sph-at-mhs.unc.edu
Date: Sat, 10 Aug 1996 9:33:49 -0400
Subject: Re: integrated microscopy reply

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Message-ID: {1D7D0C3201F70300-at-mhs.unc.edu}
In-Reply-To: {147D0C3201F70300-at-mhs.unc.edu}

I want off of this list myself. I started getting messages last night
and it seems that each one is sent in duplicate, I think that you may be
right about Madison, and yes, I did recieve some announcements from them
in the past ( but I didn't reply).


Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
University of North Carolina
CB#7400, Rosenau Hall
Chapel Hill, NC 27599-7400
Ph. 919-966-6343
Fax 919-966-6123





From: bnicklas-at-acpub.duke.edu (Bruce Nicklas)
Date: Sat, 10 Aug 1996 11:40:16 -0400 (EDT)
Subject: UNSUBSCRIBE ME

Contents Retrieved from Microscopy Listserver Archives
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UNSUBCRIBE ME !


Bruce Nicklas bnicklas-at-acpub.duke.edu
B365 LSRC Bldg. FAX: 919 613-8177
Duke University Phone: 919 613-8196
Box 91000
Durham, NC 27708-1000


"To find joy in the sky, the trees, the flowers. ...
There are always flowers for those who wish to see them."
Henri Marisse, "Jazz"







From: (Stephan Spencer) :      sspencer-at-rhino.bocklabs.wisc.edu
Date: Sun, 11 Aug 1996 02:51:06 -0500
Subject: imrlist explanation

Contents Retrieved from Microscopy Listserver Archives
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I am still not sure exactly what happened here, but it looks to me like a
misconfiguration or error in what was supposed to be a mail reflector
of a one-time IMR symposium announcement. Instead it was functioning as
a listserver when people started sending emails to it. So it turned into
something like a feedback loop.

I have been sent a number of emails from angry scientists regarding this.
Please understand that no harm was meant and this was a mistake. It's
upsetting to see messages from scientists saying "you can bet this did not
win any friends for the Integrated Microscopy Resource!". Even threats of
legal action! The IMR tries very hard to provide valuable resources to the
microscopist community, and would not knowingly set out to fill Netizens'
email boxes with junk mail.

I hope you will forgive this error. It has not happened before. It will
not happen again.

I am no longer affiliated with the IMR, so please do not send emails to me
regarding this. Instead, direct them to the IMR (vickie-at-macc.wisc.edu).

And by the way, please be gentle with Vickie, she has a baby due in a
week. (i.e. please no more calls to her home or angry emails). Please keep
in mind that this was not intentional. I know she feels very bad about
this.

I hope this answers your questions regarding this matter/disaster.

Thank you for your understanding,

Stephan Spencer
sspencer-at-netconcepts.com




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Sun, 11 Aug 1996 12:59:45 -0500 (EST)
Subject: imrlist explanation

Contents Retrieved from Microscopy Listserver Archives
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For all of you who are trying to subscribe or unsubscribe , we have the
instructions posted on our WWW page under Tips & Tricks. The URL is :

http://www.biotech.ufl.edu/~emcl/


Maybe Nestor could post them on the MSA home page as well.
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: Smith, Peter :      SMithP-at-agresearch.cri.nz
Date: Mon, 12 Aug 1996 12:18 +1200 (NZST)
Subject: Optiscan Confocals

Contents Retrieved from Microscopy Listserver Archives
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Have just received a brochure on a product called optiscan.At first glance
it looks like a laser head which can be fitted to any reasonable light
microscope and hence transform it into a confocal. Has anyone any knowledge
or experience of this product. Thanks in advance
Peter Smith




From: Nestor J. Zaluzec-Argonne Nat. Lab. :      ZALUZEC-at-aaem.amc.anl.gov
Date: Sun, 11 Aug 1996 20:59:16 -0500 (CDT)
Subject: Visit MIcroscopy & Microanalysis 96 LIVE on the WWW

Contents Retrieved from Microscopy Listserver Archives
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G'day Colleagues

Putting on my hat as Program Chair of Microscopy
and Microanalysis 1996, I'd like to invite those
members of the Microscopy Listserver community that
cannot make it to the Meeting this week to visit
us via the WWW.

Using the protocols developed for my TelePresence
Microscopy Site at ANL (http://tpm.amc.anl.gov)
I have established 2 Live Video feeds direct
from the Conference Site in the Minneapolis
Convention Center to the WWW.

You may access these Live Feeds directly from
the Home Page of the Microscopy Society of America

http://www.msa.microscopy.com

The Video Feeds offically go on-line about Noon CST
on Monday 8/12/96 at the start of the Conference
Exhibit and the opening of the Computer Workshop.
However, you may sneak preview them right now, realizing
that we are still hasseling with the usual installation
headaches.

In any event, please feel free to join in the meeting
even if it is only via a remote link. Just think of
this as another way I'm trying to bring the microscopists
of the world abit closer together. A Video Listserver
so to speak! (Yes, before you ask we can also send
audio, but I've turned that function off right now).

All reports of your connection location, video
quality, download speed as well as your comments will be
appreciated.

Just send them to me at Zaluzec-at-Microscopy.Com


Cheers....

Nestor
Your Friendly Neighborhood SysOp






From: CHINGYI-at-ccvax.sinica.edu.tw
Date: Mon, 12 Aug 1996 21:27:18 +0800
Subject: Emulsion of Ilford L4 ?

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Message-Id: {MAPI.Id.0016.00697020202020203235373830303030-at-MAPI.to.RFC822}
Priority: Normal
To: microscopy-at-Sparc5.Microscopy.Com
Cc: pearl {yip-at-maxwell.rl.plh.af.mil}
Mime-Version: 1.0

Dear all,

Does anyone know that the catalog No. of Ilford L4 emulsion for
doing EM-autoradiography ? Since I can not find this info from
the company in Taiwan.
Any help or info would be highly appreciated in advance.

***************************************************************
Min-Huei, Chen
Institute of molecular biology
Academia Sinica, Nankang,
Taipei, Taiwan
E-Mail : chingyi-at-ccvax.sinica.edu.tw
****************************************************************




From: Gavin S. Dawe :      G.Dawe-at-iop.bpmf.ac.uk
Date: Mon, 12 Aug 1996 17:18:14 +0100
Subject: Receptor antibodies

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199608121616.RAA05654-at-iop.bpmf.ac.uk}
X-Sender: spjtgsd-at-mail.iop.bpmf.ac.uk
X-Mailer: Windows Eudora Pro Version 2.1.2
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


Can anyone suggest vendors of antibodies for receptor
immunocytochemistry ? Specifically, I am interested in
antibodies to excitatory amino acidergic receptor subunits.

Thanks for any help,

Gavin.


********************************************************

Gavin S. Dawe,
Departments of Psychology and Neuroscience,
Institute of Psychiatry,
De Crespigny Park, Denmark Hill,
London, SE5 8AF,
England, UK.

Telephone: +44 (0)171-919-3356
Fax: +44 (0)171-708-3497 / 277-1390

********************************************************






From: versaci raul :      versaci-at-cnea.edu.ar
Date: Mon, 12 Aug 1996 11:56:55 -0700
Subject: JEOL, Yaichi Konishi.

Contents Retrieved from Microscopy Listserver Archives
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Message-ID: {320F7E77.1295-at-cnea.edu.ar}

} Subject: JEOL, Y. Konishi
} Date: Mon, 12 Aug 1996 11:32:57 -0700
} From: versaci raul {versaci-at-cnea.edu.ar}
} Organization: cnea
} To: microscopy-at-sparc5.microscopy.com
}
} Dear Mr. Yaichi Konishi,
} Our bidding for a analytical transmision electron microscope was answered
} by the local Philips and JEOL representatives, offering instruments in
} accordance with the requested technical characteristics. The Philips
} CM200 instruments was the cheapest offer, being about U$S 80.000 less
} than the JEOL offer for the JEM 2010. Then, according to our regulations
} we have decided for the cheapest offer. However, the local representative
} has legally observed the bidding, alleging that any change of polar
} pieces cannot be made by the operator in the Philips instrument as
} requested.
} R. Versaci
} Comision Nacional de Energia Atomica
} Departamento de Materiales
} Buenos Aires-Argentina
} E-mail: versaci-at-cnea.edu.ar




From: versaci raul :      versaci-at-cnea.edu.ar
Date: Mon, 12 Aug 1996 12:13:34 -0700
Subject: JEOL, Yaichi Konishi

Contents Retrieved from Microscopy Listserver Archives
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Message-ID: {320F825E.2783-at-cnea.edu.ar}


Dear Mr. Yaichi Konishi,

Our bidding for a analytical transmision electron microscope was answered by the local Philips and JEOL representatives, offering instruments in accordance with the requested technical characteristics. The Philips
CM200 instruments was the cheapest offer, being about U$S 80.000 less than the JEOL offer for the JEM 2010. Then, according to our regulations we have decided for the cheapest offer. However, the local representative
has legally observed the bidding, alleging that any change of polar pieces cannot be made by the operator in the Philips instrument as requested.


R. Versaci

Comision Nacional de Energia Atomica
Departamento de Materiales
Buenos Aires-Argentina
E-mail: versaci-at-cnea.edu.ar
















From: Lucio Mulestagno :      luciom-at-NEWTON.UMSL.EDU
Date: Mon, 12 Aug 1996 12:50:11 -0500 (CDT)
Subject: Re: Optiscan Confocals

Contents Retrieved from Microscopy Listserver Archives
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Hi Peter,
I haven't received the mentioned brochure, but would be very interested
to receive it. Could you send me a copy ro 800 nuberaddress I could
contact thanks.

Lucio
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Lucio Mule'Stagno
MEMC Electronic Materials Inc University of Missouri -St.Louis
Silicon Materials Research Group Physics Dept.,
501 Pearl Dr., 8001 Natural Bridge rd.,
St.Peters, St.Louis
MO 63376 MO 63121
tel: 314 279 5338 314 516 5931/3
fax: 314 279 5363 314 516 6152
email: lmulestagno-at-memc.com luciom-at-newton.umsl.edu
URL:http://www.newton.umsl.edu
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From: garyc-at-stud.unit.no (Gary)
Date: Mon, 12 Aug 1996 21:24:34 +0200 (MET DST)
Subject: 3DR

Contents Retrieved from Microscopy Listserver Archives
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Hi!

I have made some 3D reconstructions of plant cells available to the net.
There are 3DRs that I have made during my thesis. The 3DR are made using LM
sections of plant cells, transfering the sections to a PowerMac and
processing them
with NIH-Image, no other program has been used to render the 3DRs.
Please if you take a look to my page, let me know what you think.

Any comments would be appreciated. Correct my grammar if it is wrong.

The reconstructions can be found in the following URL:

http://www.nvg.unit.no/~gary

under thesis.

To see the animation you have to use NETSCAPE 2.0 or higher. If you try to
see the S/MM-03 part, it will be very slow, because the reconstruction is
to big. I will try to make this 3DR a little smaller.

thanks...

/////////////////////////////////////////////////////////////////
// // //

// Gary Chinga // email :garyc-at-james.avh.unit.no //
// Plantebiosenteret // WWW :http://www.nvg.unit.no/~gary //
// NTNU, 7055 Dragvoll // phone : 73590168 //
// Norway // fax : 73590177 //
// // //
/////////////////////////////////////////////////////////////////






From: wise-at-vaxa.cis.uwosh.edu
Date: Mon, 12 Aug 1996 16:16:10 +0000
Subject: Plans for Light Boxes?

Contents Retrieved from Microscopy Listserver Archives
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Does anyone have readily available plans for a home made light box for
inspection of negatives? Desktop or wall mounted? I could probably design
one myself but am trying to avoid re-inventing the wheel.

Thanks in advance,

Bob

Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-vaxa.cis.uwosh.edu






From: Gavin S. Dawe :      G.Dawe-at-iop.bpmf.ac.uk
Date: Mon, 12 Aug 1996 17:18:14 +0100
Subject: Receptor antibodies

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone suggest vendors of antibodies for receptor
immunocytochemistry ? Specifically, I am interested in
antibodies to excitatory amino acidergic receptor subunits.

Thanks for any help,

Gavin.


********************************************************

Gavin S. Dawe,
Departments of Psychology and Neuroscience,
Institute of Psychiatry,
De Crespigny Park, Denmark Hill,
London, SE5 8AF,
England, UK.

Telephone: +44 (0)171-919-3356
Fax: +44 (0)171-708-3497 / 277-1390

********************************************************






From: Diganta Saha :      saha-at-engin.umich.edu
Date: Mon, 12 Aug 1996 18:33:31 -0400 (EDT)
Subject: Re: JEOL, Yaichi Konishi.

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Excuse me, I have no idea why or how I received email from the

microscopy-at-sparc5.microscopy.com email list but I have very little to
do with anything biological. If there is someone who is responsible
for this list can you kindly take me off.
-Diganta

+----+ +----+ +-----------------------+-----------------------------+
| \ GO / | | Diganta Saha | Material Science Engineer, |
++ + \ / + ++ | | Virtual Reality Lab Staff, |
| |\ \/ /| | | Forest Place #205 | ITD Level 1 Consultant, |
| | \ / | | | 721 South Forest Av. | Hermes Project Webmaster, |
| | \ / | | | Ann Arbor, MI 48104 | Home :1-(313)-668-8154 |
++ ++ \/ ++ ++ |-----------------------+-----------------------------|
| | BLUE | | | URL: http://www-personal.engin.umich.edu/~saha |
+----+ +----+ +-----------------------------------------------------+






From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Mon, 12 Aug 1996 19:16:00 -0500
Subject: Two Symposia to be broadcast live on Tuesday from MM96

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199608130004.TAA16069-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Colleagues

Hello again from Minneapolis. We have a news flash for
you.

Tomorrow Tuesday 8/13/96 we will be broadcasting
live the complete TelePresence Microscopy Symposium
on the WWW. I will be reconfiguring the image display
windows to smaller sizes and attempting to add the
audio feed at the same time. This is an experiment
but if you have access to a high speed connection to
the internet you may be able to login and watch and
in some cases listen to the symposium. (Donot attempt
to watch and listen over a modem, I recommend at least
ISDN, but better yet find someone with a T1 or better
connection. Also use the lastest version of NetScape
that you can download I recommend at least Version
2.01)

In addition to the TPM symposium, Congressman
Bill Luther, Member of the House Science Committee
and the Basic Research Subcomittee will be giving
a presentation entitled

"Perspectives on Federal Funding for Science"

This will also be put on-line, but via delayed
broadcast also on Tuedsay. We expect Congressman
Luthers talk to be broadcast between about 3:30 to
4:00 and to last roughly 20 minutes.

Direct links to both of these presentations can be found
on the MSA HomePage.

http://www.msa.microscopy.com


Cheers.... ( and I hope this experiment works, or
at least gives us an idea of what we can/will
be able to do in the future)

Nestor

Your Friendly Neighborhood SysOp.






From: CHINGYI-at-ccvax.sinica.edu.tw
Date: Mon, 12 Aug 1996 21:27:18 +0800
Subject: Emulsion of Ilford L4 ?

Contents Retrieved from Microscopy Listserver Archives
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Microscopy-at-sparc5.microscopy.com (MICROSCOPY MSA list messages)
Message-ID: {1996Aug13.104200.1814.9177-at-missgate.sunderland.ac.uk}
X-Mailer: Microsoft Mail via PostalUnion/SMTP for Windows NT
Mime-Version: 1.0
Content-Type: text/plain; charset="US-ASCII"
Organization: University of Sunderland


Dear CHINGY

your best bet would be to contact a specialist e.m. supplier rather than
Ilford Photographic. You could try someone like:-

Agar Scientific
66a Cambridge Road
STANSTED
Essex
CM24 6DA
ENGLAND
Telephone (01279) 813519
Fax (01279) 815106 } N.B. I think international calls are
prefixed by 0044
and miss the first
0.

The Agar Scientific catalogue number for 50mls of Ilford nuclear emulsion
L4 is P9282 and the price was 111.00 UK pounds (excluding VAT - Value Added
Tax - and delivery) in January 1995 but there may be a surcharge. If you ask
nicely they should send you a catalogue.

Good luck.

Malcolm Haswell
University of Sunderland
UK
e-mail:- es0mhs-at-environment.sunderland.ac.uk

----------






From: philf-at-NEWTON.UMSL.EDU (Phil Fraundorf)
Date: Tue, 13 Aug 1996 06:36:07 -0500
Subject: A small animation

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A short "gif-animation", with power-spectrum accompaniment, of one path
for finding Scherzer defocus on a holey-film specimen is now accessible
through our browser-interactive simulator at
{http://www.umsl.edu/~fraundor/epc/index.html} . Problem is: You have to
find Scherzer defocus on the unknown in order to get it!

Enjoy. /philf :)

\//
(-at- -at-)
//\/\/\/\--o00-(_)-0oo--}
// P.Fraundorf Phys&Astr/CME 3145165044 philf-at-newton.umsl.edu
\\ U.Missouri-St.Louis MO 63121 http://www.umsl.edu/~fraundor
\\/\/\/\/\/\/\/\/-----------------}





From: benchaib-at-rockefeller1.univ-lyon1.fr (Mehdi BENCHAIB)
Date: Tue, 13 Aug 1996 17:43:18 +0200 (MET DST)
Subject: A small animation

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subscribe microscopy benchaib-at-rockefeller1.univ-lyon1.fr





From: Brad Goodwin :      goodwib-at-wdni.com
Date: Tue, 13 Aug 1996 08:18:08 -0700
Subject: LR White Accelerator

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Message-Id: {199608131518.AA03303-at-interlock.wdni.com}

Does anyone use LR White with plant tissue? I'm trying to use it, but I
don't have any instructions for the ratio of accelerator to resin. Any
help would be greatly appreciated.




From: Lesley S. Smith :      lesleys-at-pobox.upenn.edu
Date: Tue, 13 Aug 1996 13:35:29 -0400 (EDT)
Subject: Canadian Microscopy Courses

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Hi Susan,

There is an entire listing of all courses offered in Canada
through the Microscopical Society of Canada. It was compiled by David
O'Neil. his email address is : oneild-at-imb.lan.nrc.ca

The latest listing I have is Jan. 95. It should be very current
and comprehensive. The "Bulletin", which comes out 4 times a year also
lists short courses and workshops. The editor is Carolyn Emerson. Her
email address is: cemerson-at-kean.ucs.mun.ca

The MSC also has local sections. The Atlantic sEction is very
active and meets regularly. Again, David O'Neil could give you more info
on that.

Good Luck!! I am a Nova Scotian in case you're wondering how I
fit into this!

Lesley Bechtold
lesleys-at-pobox.upenn.edu




From: IAN HALLETT :      ihallett-at-MARCCRI.MARC.CRI.NZ
Date: Wed, 14 Aug 1996 08:58:25 GMT+1200
Subject: Re: LR White Accelerator

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Resent-Message-Id: {199608131400.JAA24662-at-mercury.acs.unt.edu}
Resent-from: "David Garrett" {DGARRETT-at-gab.unt.edu}
Resent-to: Microscopy-at-Sparc5.Microscopy.Com
Resent-date: Tue, 13 Aug 1996 09:00:38 CST6CDT

} Date: Tue, 13 Aug 1996 08:18:08 -0700
} From: Brad Goodwin {goodwib-at-wdni.com}
} Organization: Weyerhaeuser
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: LR White Accelerator


} Does anyone use LR White with plant tissue? I'm trying to use it,
} but I don't have any instructions for the ratio of accelerator to
} resin. Any help would be greatly appreciated.


Brad

The data sheets for LR White recommend one drop of accelerator in
10ml resin with curing in 10-20 minutes. Points to watch are to
cool the block during the polymerisation and not to use osmium
tetroxide to post-fix material for EM. If the blocks polymerise too
quickly or too slowly adjust the accelerator volume slightly (it can
go off in time).

We use LR White extensively for LM and immuno-gold TEM of plant
material with good success though we normally use thermal
polymerisation.

Ian Hallett




Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660
EMail ihallett-at-hort.cri.nz




From: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
Date: Tue, 13 Aug 1996 18:35:12 -0600
Subject: Help needed: Te, Tb, Bi stds

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Message-Id: {v01530500ae36cdf658ce-at-[130.39.132.39]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Can anyone provide me some information about the possible sources
for Te (Tellurium), Tb (Terbium), and Bi (Bismuth) standards for metal
microprobe analysis? Or any commercially available materials that can be
used for the purpose?

Thanks for your help.

Xiaogang

***********************************
* Xiaogang Xie *
* SEM & Microprobe lab *
* Dept. of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
***********************************






From: Patricia Musa :      pmusa-at-udel.edu
Date: Wed, 14 Aug 1996 09:25:20 -0400 (EDT)
Subject: TEM Microscopist Position Available

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Electron Microscopist Position - Magnetic Materials
University of Delaware

The Department of Physics and Astronomy at the
University of Delaware invites applications for an
electron microscopist position in an interdisciplinary
program supported by the Department of Defense involving
the fabrication, characterization, study, and
development of advanced high temperature magnetic
materials. Previous experience in analytical/high
resolution electron microscopy and Lorentz microscopy is
required. Position start date is October/November 1996.
Applications will be accepted until Oct. 16, 1996.
Applicants should send a resume and the names and
addresses of three references to: George C.
Hadjipanayis, Department of Physics and Astronomy,
University of Delaware, Newark, DE 19716-2570, USA.
The University of Delaware is an Equal Opportunity
Employer which encourages applications from qualified
members of minority groups and women.


****************************************************************************
Patricia Musa pmusa-at-udel.edu
Physics & Astronomy Dept.
University of Delaware Phone: 302-831-2662
Newark, DE 19716-2570, USA FAX: 302-831-1637
*****************************************************************************





From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Wed, 14 Aug 1996 08:13:59 -0500
Subject: Unsubscribe Off-Line

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Sorry Folks,,

Unsubscribe is off-line for some reason. I won't be able
to get a fix in until tomorrow sometime.

Nestor

Your Friendly Neighborhood SysOp






From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Wed, 14 Aug 1996 10:01:36 -0500
Subject: Unsubscribe Off-Line

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
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I am posting this for a colleague, PLEASE DO NOT REPLY TO ME, REPLY TO:
SBME-at-RDC.PUC-RIO.BR.
Any replies to be will end up in the trash!

Research Associate and Post-Doctoral Postions

Available in Rio de Janeiro - Brazil

Requirements: Ph. D. in Materials Science and Engineering or Physics
with hands-on experience in experminetal AEM and HREM of metallic and
ceramic materials. Highly desired experience with JEOL 2010 TEM and
with image processing operations.

Task: Co-ordinate preparation of bulk and cross-section samples. Operate
(and give demonstrations to students) a JEOL 2010 TEM 960 SEM. Strong
and interactive participation in research projects involving detailed
microstructural characterization of engineering materials and minerals.

Salary: According with experience, comparable to equivalent position in
the US. Round trip airplane ticket is provided. One year or two years
appointments with possibility of renewal.
Please contact and mail your resume to:

Prof. Guillermo Solorzano
President
Brazillian Society for Electron Microscopy
C.P. 38090 - Gavea
22452 Rio de Janeiro
Brazil

Fax: +5521-5112489 / 5112196
e-mail: SBME-at-RDC.PUC-RIO.BR






From: Ron Neumeyer :      micron-at-bc.sympatico.ca
Date: Wed, 14 Aug 1996 06:14:43 -0700 (PDT)
Subject: unsubscribe

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From: weiliang EARTH.WGONG.PLANET. gong :      wgong-at-UNM.EDU
Date: Wed, 14 Aug 1996 22:19:14 -0600 (MDT)
Subject: unsubscribe

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From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: 15 Aug 96
Subject: Help needed: Te, Tb, Bi stds

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Message-Id: {199608150810.EAA21350-at-mime2.prodigy.com}
X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc04

-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #3.0 ] --

Xie Xiaogang wrote:
===========================
Can anyone provide me some information about the possible
sources
for Te (Tellurium), Tb (Terbium), and Bi (Bismuth) standards for metal
microprobe analysis? Or any commercially available materials that can be
used for the purpose
===========================
SPI Supplies produces these kinds of standards for microanalysis, and
full details including prices are available at our website given below.
Just click on the catalog cover, and once in the Table of Contents,
click on "Standards and Calibration Aids".

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.
com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.
com


Look for us!
############################
WWW: http://www.2spi.com
############################
=====================================================









From: David Brauer :      dbrauer-at-asrr.arsusda.gov
Date: Thu, 15 Aug 1996 11:50:50 -0400 (EDT)
Subject: Help needed: Te, Tb, Bi stds

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subscribe dbrauer-at-asrr.arsusda.gov




From: Woody.N.White-at-mcdermott.com
Date: 15 Aug 96 11:59:00 -0500
Subject: Kevex/printer drv?

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Hello out there...

I am looking for a printer driver to permit my Kevex 8000/Delta to
dump to a late model color inkjet or possibly a laserjet. The system
is a DEC PDP 11/73 running RT11 w/TSX. Currently I have a
(malfunctioning) Tektronix 4696 ink jet printer connected through the
parallel port. Application programs of interest include "Advanced
Imaging", "Automated Image Analysis (AIA), and Quantx.

Any home-grown drivers for my orphan?

Thanks in advance, Woody

woody.n.white-at- mcdermott.com (alt: woody.white-at-worldnet.att.net)

N. W. White, Jr
Babcock & Wilcox Co.
Lynchburg Research Center
Lynchburg, Va. USA




From: msi4-at-cornell.edu (Michael Isaacson)
Date: Thu, 15 Aug 1996 22:24:43 -0400 (EDT)
Subject: unsubscribe

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unsubscribe msi4-at-cornell.edu

Michael S. Isaacson
Associate Dean for Research & Graduate Studies
College of Engineering
241 Carpenter Hall
Cornell University
Ithaca, NY 14853
Telephone: (607)255-9545
Fax: (607)255-9606






From: joan.clark-at-sci.monash.edu.au (Joan Clark)
Date: Fri, 16 Aug 1996 15:43:05 +1000
Subject: Mosquito photograph wanted

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Would anyone have a SEM micrograph of the mosquito "aedes aegypti". An
advertising company are looking for one and are prepared to pay for it. If
you are interested please let me know
Thank you
Joan Clark

Joan Clark
Department of Zoology
University of Melbourne
joan.clark-at-sci.monash.edu.au






From: Probing & Structure :      pns-at-ultra.net.au
Date: Fri, 16 Aug 1996 18:43:58 +1000
Subject: Re: Help needed: Te, Tb, Bi stds

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} Date: Thu, 15 Aug 1996 15:06:21
} To: xxie-at-lsuvm.sncc.lsu.edu (Xiaogang Xie)
} From: Probing & Structure {pns-at-ultra.net.au}
} Subject: Re: Help needed: Te, Tb, Bi stds
}
} At 18:35 13-08-96 -0600, you wrote:
} } Can anyone provide me some information about the possible sources
} } for Te (Tellurium), Tb (Terbium), and Bi (Bismuth) standards for metal
} } microprobe analysis? Or any commercially available materials that can be
} } used for the purpose?
} }
} } Thanks for your help.
} }
} } Xiaogang
} ****************************************************
Of course P&S and several other EM/Probing suppliers too can supply those
standards.
The advice is shop around, standard blocks prices and quality differ.
The term produce should mean manufacture, but some people use it as in "he
produced a rabbit out of a hat."
P&S supplies and we do not pretend to manufacture what we do not. Compare
when you shop and you may find that some products are in fact of identical
manufacture but have only been re-badged - at great cost.

Jim Darley
Probing & Structure
Microscopy Supplies & Accessories

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site http://www.ultra.net.au/~pns/
***********************





From: Kevin Mackenzie :      nhi691-at-abdn.ac.uk
Date: Fri, 16 Aug 1996 10:01:35 +0100 (bst)
Subject: SCOTTISH MICROSCOPY MEETING

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The 24th SCOTTISH MICROSCOPY GROUP SYMPOSIUM
(First circular)

ABERDEEN EXHIBITION AND CONFERENCE CENTRE
Wednesday 13 November 1996.

This year the meeting will emphasise on techniques rather than results.

The following topics have been selected:

(a) Biological Immunocytochemistry

(b) Energy Filtering Electron Microscopy

(c) Environmental Electron Microscopy

(d) Image Archiving

These talks will be interspersed with several short presentations. The
names of the speakers and titles of the talks will be sent out with the
second circular.

As in previous years, we welcome posters and there will be prizes for
the best entries.

If you are interested in submitting a poster or would like more
information please email me.




Kevin Mackenzie
Tillydrone E.M. Unit
University of Aberdeen
Tillydrone Avenue
Aberdeen
Scotland
Tel 01224-272847
fax 01224-272396
email k.s.mackenzie-at-abdn.ac.uk







From: slimbach-at-facstaff.wisc.edu (steve limbach)
Date: Fri, 16 Aug 1996 09:06:17 -0600
Subject: Image file management software

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I need some recommendations! I'm presently looking for software,
preferably mac or mac/pc crossplatform, for keeping track of images
created in our lab. The ideal software would be able to index any image
file type, create postage stamp size images, and sort by a variety of
criteria, such as date, experiment, researcher, etc. Additionally it
should be able to read opticals, cd's, or any other scsi device.
What do you use? Pro's? Con's?

Fetch? Filemaker?

Thanks for the help! Steve

University of Wisconsin-Madison
Robert M. Bock Laboratory
1525 Linden Dr
rm#527
Madison, WI 53706

Work (608) 262-4581
Fax (608) 262-4570
Home (608) 837-9566

slimbach-at-facstaff.wisc.edu






From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Fri, 16 Aug 1996 12:02:07 -0400 (EDT)
Subject: collagen banding

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I was wondering if anybody would know of a good reference for looking at
the d.periodicity of collagen banding in rabbits.

Thanks,

Phil 8-{)




From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Fri, 16 Aug 1996 12:22:49 -0500
Subject: Microscopy & Microanalysis-96

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Message-Id: {199608161717.MAA08414-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


A final farewell & thanks to Minneapolis......

Well the Microscopy & Microanalysis 96 meeting is over and
a lot of people are happy and rightfully tired.

Allow me to take this opportunity to literally take my hat off
to all those of you who have made this meeting a resounding
success. A special acknowledgement is due to the Symposium Organizers
and very importantly the Local Arrangements Committee,
without whom this meeting could not have taken place.
You all did a great job!

For those of you that could not attend, this year the scientific
registration reached 1530 individuals and is the second highest
ever attendance in the meeting history, exceeded only by the
mega-meeting which was held in Boston in 1992 and was
also the 50th anniversary meeting of the Microscopy Society
of America.

Several other notable "First's" happened this year.

The proceedings appeared on-line and many of you took the
opportunity to preview medium resolution copies of all the
presentations.

Poster Sessions were distributed throughout the meeting
and about 1/4 of each were presented daily, with
each poster presenter given a brief opportunity
to stand up and say a few words in a topical discussion session.

The experimental TelePresence link which we established and
only announced on the evening prior to the start of the meeting
met with surprizing success. This represented the first
ever Live Internet broadcast to the world from a Microscopy
meeting. And given the short notice I'm particuliarly pleased
with the results. In effect, we are now becoming a truely
connected community of microscopists and microanalysts.

We recorded TelePresence Access from 18 countries around
the world the farthest were our colleagues in Australia
while the most numerous were various Universities in the USA.
For the record we had "on-line" visits from (in no particuliar order) :

Australia, Japan, Sweden, Denmark, France, Mexico,
Hungary, Germany, Canada, New Zealand, United Kingdom, Brazil,
Switzerland, Finland, Belgium, Norway, Solvenia and of course many
links from the USA in government, university and commerical
organizations.

A total of 1252 logins were recorded to the TelePresence link in
the computer workshop area, where we not only broadcast
a continuous view of the workshop, but on Tuesday
broadcast live the entire TelePresence Microscopy Symposium
(another first), with yours truely being the first speaker ;-) .
Those who had access to a Java Aware WWW browser were able to not only
watch but listen to the symposium on-line (with varying degrees
of success depending upon the speed of your connection to
the internet).

We also had delayed broadcast on Tuesday of the public policy
symposium where presentations were made by Congressman
Bill Luther, member of the House Science Committee and by
Christopher Roosa, the Assistant Legislative Director of the
House Committee on Science. I will be arranging a repeat
broadcast of that discussion for those of you who might
be interested and will post the details sometime in the next
few weeks.

In addition, to the computer workshop we recorded
883 acesses to the Exhibit Floor Telepresence line.
Because of our connectivity, a number of Exhibitors were
able to have live high speed access (T1) to the Internet, yet
another first, and I expect this to grow next year.

Finally on behalf of the Microscopy Society of America, the
Microbeam Analysis Society and the Microscopical Society of Canada,
the meeting attendee's and myself allow me to thank again
all those who made the meeting a success. We all hope that if
you couldn't make it this year you will be able to
join us next year in Cleveland, Ohio (August 10-14, 1997).
Just keep checking the society home page (http://www.msa.microscopy.com)
for details.

Cheers.... Nestor

Your Friendly Neighborhood SysOp

and now happily

Former Program Chair of Microscopy & Microanalysis !!!






From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Fri, 16 Aug 1996 12:40:26 -0500
Subject: Re: Image file management software

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Message-Id: {v01540b00ae3a60794534-at-[128.206.15.189]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I used to use Kodak's Shoebox but have just upgraded to Canto's Cumulus
desktop image database. I haven't had time to install it yet but it is
supposed to do everything you ask. It is apparently a major player in the
field based on the user list they supplied in the original advertisement
which I no longer have but I think they listed Disney's Animation studio
and several big graphic imaging companies. My upgrade from Shoebox pricing
was only $29.95 but it lists for about $99 I think. You might want to give
them a call at 415-905-0300 or fax 415-905-0315. I hope it is as good as
it sounds.


} I need some recommendations! I'm presently looking for software,
} preferably mac or mac/pc crossplatform, for keeping track of images
} created in our lab. The ideal software would be able to index any image
} file type, create postage stamp size images, and sort by a variety of
} criteria, such as date, experiment, researcher, etc. Additionally it
} should be able to read opticals, cd's, or any other scsi device.
} What do you use? Pro's? Con's?
}
} Fetch? Filemaker?
}
} Thanks for the help! Steve
}
} University of Wisconsin-Madison
} Robert M. Bock Laboratory
} 1525 Linden Dr
} rm#527
} Madison, WI 53706
}
} Work (608) 262-4581
} Fax (608) 262-4570
} Home (608) 837-9566
}
} slimbach-at-facstaff.wisc.edu

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(314)-882-4712 (voice)
(314)-882-0123 (fax)






From: Woody.N.White-at-mcdermott.com
Date: 8/16/96 9:56 AM
Subject: Re: Kevex/printer drv?

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Hi Dave,

I know what you mean.... dumping images to the 4696 using the
available software is a total waste of time. The default resolution
of the original is 512x256 (x256 shades). I have had success in
producing nice images, but had to work for them...I don't do it
regularly.

Also, Tektronix has discontinued sales and service contracts on the
4696. They still will repair them "on a best effort basis, based on
time and materials, continginent on parts availability" (almost
accurate quote).

In AIA, you can set the resolution to 512x512 (or more). After the
image has been collected, I save as tiff to 44 Mb bernoulli.
Since my sys is not wired to network, I take the 44 to a PC which I
have configured to read RT-11 format 44 Bernoullis, copy to dos/win,
then print using PC software to (at least) a 600dpi laserjet. For
halftoned images, a 1200dpi would be better yet (software permiting).

For spectra, I scrounged a Hewlet Packard plotter. The driver was on
my system for a different model HP plotter (HPPLOT), but the commands
were the same. Not only is the plotter faster, but I think the
appearance is improved. The only drawback is I cannot "compare" two
spectra in two (transparent) colors and have the overlap as a third
color.

BTW All:

I know the right video printer will work, but have not (yet) recieved
permission to purchase one. Furthermore, (given the 8000 display) the
quality/resolution will never be as good for image printing as a GOOD
high res digital ptr could be.


Woody

Wk: woody.n.white-at-mcdermott.com
Hm: woody.white-at-worldnet.att.net When the m-server is running ha ha!



______________________________ Reply Separator _________________________________


Woody,
I have the same Kevex setup, and use the 4696 to print spectra.
However, images from AIA and feature packages look like crap.
I would love to print to an HP or similar inkjet. Please let
me know if you get any responses to your request.
Thanks,


Dave Gnizak
Ferro Corporation
Technical Center
Independence, Ohio




From: Sara Miller :      saram-at-acpub.duke.edu
Date: Fri, 16 Aug 1996 20:54:20 -0400 (EDT)
Subject: Re: Microscopy & Microanalysis-96

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Wow, Nester!!!
The MSA computer presence is impressive---and most of it due to you.
Many thanx for everything from all of us.

Sincerely,
Sara



Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: LINDSAY UNSWORTH :      l.unsworth-at-student.qut.edu.au
Date: Sun, 18 Aug 1996 11:52:18 +1000 (EST)
Subject: SEM: Prep of Aquatic samples - topographic studies.

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Hello,
I am trying to find info on the preparation of a pond water sample.
I was wondering if critical point drying or standard preparation would be
better, considering diverse microorganisms present(diatoms, filamentous
bacteria, zooplankton, cyanobacteria, algae and protozoa). I've so far
only been able to find general information on SEM applications and the
problems of dealing with structural water, the info is also quite old.
Any useful libraries with online resources would also be greatly
appreciated.
Thanks muchly, Lindsay.





From: luciom-at-NEWTON.UMSL.EDU (Luciano Mule'Stagno)
Date: Mon, 19 Aug 1996 13:01:53 -0500
Subject: JOBS - B.S./M.S.

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The Silicon Materials Research Department at MEMC Electronic Materials Inc,
has 2 immediate openings for "engineers" . MEMC is located outside St.
Louis, and is the largest manufacturer of silicon wafers for the electronic
industry outside Japan. The St. Peters plant currently has over 2000
employees including most of the researchers, and is the fastest growing
employer in the state of Missouri.

If you or anyone you know is interested in one of these jobs, please have
them contact me or send me a resume.

The first position involves the managing of a research lab with various
equipment such as an FTIR, lifetime scanner, furnaces etc.. The person would
be responsible for ensuring that the equipment is in working order, and that
procedures are written for operating the various equipment. He/she would
also interact with various technicians, and at times might be required to
supervise their work. This person would also be required to help the
scientist to whom he reports put together data and reports.

The requirements for the job:
B.S/M.S. in Physics, Chemistry, Engineering, Material Sc., or related field.
Ph.D. not required.
Strong communication, leadership and laboratory skills required.
2+ years experience in industry (ideally the electronics industry)or
military experience preferred but not required.


The second position involves the actual running of 2-3 IR scattering
instruments (with the help of one or more technicians) and providing the
data for analysis. It will also involve TEM SEM and similar specimen
preparation. Light microscopy and image analysis would also be part of this
person's duties. The person would be responsible for ensuring the running of
the instruments, writing-up relevant procedures for technicians to use, and
helping scientists with the data analysis. He or she would also work closely
with technicians, and might be asked to supervise their activities.

The requirements for the job are:

B.S/M.S. in Physics, Chemistry, Engineering, Material Sc., or related field.
Ph.D. not required.
Specimen preparation experience preferred.
Strong communication, leadership and laboratory skills required.
2+ years experience in industry (ideally the electronics industry)or
military experience preferred but not required.


Dr. Luciano Mule'Stagno
MEMC Electronic Materials Inc.,
Silicon Materials Research Group
501 Pearl Dr.,
St.Peters,
MO 63376
tel: 314 279 5338
fax: 314 279 5363
email: lmulestagno-at-memc.com


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Lucio Mule'Stagno
MEMC Electronic Materials Inc University of Missouri -St.Louis
Silicon Materials Research Group Physics Dept.,
501 Pearl Dr., 8001 Natural Bridge rd.,
St.Peters, St.Louis
MO 63376 MO 63121
tel: 314 279 5338 314 516 5931/3
fax: 314 279 5363 314 516 6152
email: lmulestagno-at-memc.com luciom-at-newton.umsl.edu
URL:http://www.newton.umsl.edu
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From: leapman-at-helix.nih.gov (Richard Leapman)
Date: Mon, 19 Aug 1996 14:57:59 +0000
Subject: Postdoctoral Fellowship at the NIH

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Message-Id: {199608191956.PAA20969-at-helix.nih.gov}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Postdoctoral Fellowship in Analytical Electron Microscopy
at the National Institutes of Health, Bethesda, Maryland

________________________________________________________

Postdoctoral fellowship (Ph.D. in biophysics, physics or related
discipline) is now available for the development of biological electron
energy loss spectroscopy and energy-filtering electron microscopy, and the
applications of these techniques to biomedicine in collaboration with other
NIH laboratories. This collaborative research will be centered around a
field-emission scanning transmission electron microscope (VG HB501)
equipped with a parallel-detection electron spectrometer and interfaced to
a digital image acquisition system. A fixed-beam transmission electron
microscope (CM120) equipped with an imaging filter and a cooled slow-scan
CCD camera is also available.


For further information contact:

Dr. Richard Leapman
Biomedical Engineering & Instrumentation Program, NCRR
Bldg. 13, Rm. 3N17
NIH, Bethesda, MD 20892
Tel: (301) 496-2599
FAX: (301) 496-6608
e-mail: leapman-at-helix.nih.gov

(NIH is an Equal Opportunity Employer)






From: michaela :      Michaela.Smith-at-sci.monash.edu.au
Date: Tue, 20 Aug 1996 14:02:57 GMT+10
Subject: freeze-sub - plant leaf tissue

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Hello can anyone help me? I am having resin infiltration problems using
freeze-substitution on leaf tissue from Myrothamnus flabellifolia.

the freeze-substitution schedule used was:

1) samples were plunge frozen in liquid propane.
2) samples were then substituted in 100% methanol for 14 days at
-82 C.
3) samples were then placed in a cryochamber and the temperature
was ramped up from -80 C to -25 C over a 45 hr period.
4) samples were infiltrated in two changes of 100% LR Gold resin (+
benzil) at -25 C with each change for a duration of 1 hr.
5) samples were then placed in 100% LR Gold (+benzil) at -25 C for
16 hr.
6) samples were then placed into another change of 100% LR Gold
(+benzil) for 248 hr at -20 C.
7) samples were polymerized in a final change of 100% LR Gold (+
benzil) under UV light at -25 C for 24 hr.

The blocks polymerized fine but there seemed to be no resin infiltration
into the tissue at all - the leaf was a spongy mass contained within the
polymerized block of resin.

Is this freeze-substitution schedule OK?
Is this freeze-substitution schedule suitable for leaf tissue? Is there
a tried freeze-substitution protocol for plant leaf tissue?
Are there any ideas on enhancing resin infiltration into leaf tissue?

thanks for your time, Michaela.





From: michaela :      Michaela.Smith-at-sci.monash.edu.au
Date: Tue, 20 Aug 1996 14:02:57 GMT+10
Subject: freeze-sub - plant leaf tissue

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Microscopy-at-Sparc5.Microscopy.Com (MICROSCOPY MSA list messages)
Message-ID: {1996Aug20.094300.1814.18719-at-missgate.sunderland.ac.uk}
X-Mailer: Microsoft Mail via PostalUnion/SMTP for Windows NT
Mime-Version: 1.0
Content-Type: text/plain; charset="US-ASCII"
Organization: University of Sunderland


Dear Michaela

I am not familiar with LR Gold in freeze substitution but I have seen
schedules for Lowicryl which involve the use of methanol/resin mixtures
between 100% methanol and 100% resin. This would seem sensible for any good
infiltration, especially of plant, so perhaps your stage 4 should include:
25:75 resin:methanol
50:50 resin:methanol
75:25 resin:methanol
I'm sorry I can't suggest any times (possibly 1 hour each).

Malcolm Haswell
E.M. Unit
University of Sunderland
U.K.
e-mail: es0mhs-at-sunderland.environment.ac.uk

----------

Hello can anyone help me? I am having resin infiltration problems using
freeze-substitution on leaf tissue from Myrothamnus flabellifolia.

the freeze-substitution schedule used was:

1) samples were plunge frozen in liquid propane.
2) samples were then substituted in 100% methanol for 14 days at
-82 C.
3) samples were then placed in a cryochamber and the temperature
was ramped up from -80 C to -25 C over a 45 hr period.
4) samples were infiltrated in two changes of 100% LR Gold resin (+
benzil) at -25 C with each change for a duration of 1 hr.
5) samples were then placed in 100% LR Gold (+benzil) at -25 C for
16 hr.
6) samples were then placed into another change of 100% LR Gold
(+benzil) for 248 hr at -20 C.
7) samples were polymerized in a final change of 100% LR Gold (+
benzil) under UV light at -25 C for 24 hr.

The blocks polymerized fine but there seemed to be no resin infiltration
into the tissue at all - the leaf was a spongy mass contained within the
polymerized block of resin.

Is this freeze-substitution schedule OK?
Is this freeze-substitution schedule suitable for leaf tissue? Is there
a tried freeze-substitution protocol for plant leaf tissue?
Are there any ideas on enhancing resin infiltration into leaf tissue?

thanks for your time, Michaela.


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From: KRISTIN.BECKER-at-spcorp.com (KRISTIN BECKER)
Date: Tue, 20 Aug 1996 07:47:42 -0400
Subject: EM Exam - Certification

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Mime-Version: 1.0

I'm thinking about taking the EM exam for Certification and would like
to hear from anyone who has taken it recently. Any information on
what to study or helpful hints for taking the exam would be greatly
appreciated.

Thanks!! Kristin Becker
201-579-4394
kristin.becker-at-spcorp.com




From: Dave King (607)757-1248 T37/257-3 Ext DEKING-at-VNET.IBM.COM
Date: 20 Aug 1996 09:39:08 EDT
Subject: What's the EM Exam?

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Message-Id: {199608201353.IAA01159-at-Sparc5.Microscopy.Com}

To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}


As an industry employed microscopist, I'd be interested to know
what the EM exam is that Kristin Becker asked about. Is it
related to government employment?

. {
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: DR CHARLES A GARBER :      GVKM07A-at-prodigy.com
Date: Sat, 17 Aug 1996 02:29:57, -0500
Subject: Microscopy & Microanalysis-96

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Hi EM folks,
Am passing along a message from Chuck Garber seconding kudos for
Nestor. Also, included is recognition for John Mansfield, whom I forgot
to mention. Hope all of you interested in digital microscopy heard John's
talk. It was very informative and well-organized!!! And for those of
you, like I, who couldn't write fast enough, it was on the MSA computers
under digital microscopy.
Sara

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735

---------- Forwarded message ----------

-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #3.0 ] --

Hi Sara,

What makes it all the more amazing is that during all of this time,
Nestor was doing another "first", something never done before at any
exhibition we have ever participated. He made it possible to have a T1
connection run into individual exhibit booths, and novices like myself
made enormous demands not only on Nestor's time but also that of John
Mansfield.

This enabled us for example, to demonstrate the wonders of our website
and electronic catalog at T1 speeds.

And this was all done with out a single break in the cadence of his
running of the meeting!

Chuck

PS: Message sent only to you and not to the listserver.



Wow, Nester!!!
The MSA computer presence is impressive---and most of it due to you.
Many thanx for everything from all of us.
-------- REPLY, Original message follows --------

} Date: Friday, 16-Aug-96 10:10 PM
}
} From: Sara Miller \ Internet: (saram-at-acpub.duke.edu)
} To: Garber, Charles A. \ PRODIGY: (GVKM07A)
}
} Subject: Re: Microscopy & Microanalysis-96
}
} Sincerely,
} Sara
}
}
}
} Sara E. Miller, Ph. D.
} P. O. Box 3020
} Duke University Medical Center
} Durham, NC 27710
} Ph: 919 684-3452
} FAX: 919 684-8735
}
}
}

-------- REPLY, End of original message --------








From: versaci raul :      versaci-at-cnea.edu.ar
Date: Tue, 20 Aug 1996 14:41:39 -0700
Subject: JEOL, after?

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Message-ID: {321A3113.1D7C-at-cnea.edu.ar}

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From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Tue, 20 Aug 1996 17:21:07 +0000
Subject: SEM & ruptured cells

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Message-Id: {s219f3d5.053-at-wpo.nerc.ac.uk}
X-Mailer: Novell GroupWise 4.1

Hello all

We have a problem with settled phagocytic blood cells from
the mussel (Mollusca: Lammellibranch: Mytilus) which
are ruptured after critical point drying . The cells have very
thin, delicate sheets of cytoplasm around the nuclear area
which almost merge into the background. These split but so
too can the thicker nuclear area.

The cells are settled onto glass cover slips, fed bacteria,
fixed in glutaraldehyde, dehydrated with acetones (typically
30, 50, 70, 90 and 100%) and then go into liquid carbon
dioxide for drying. I know shrinkage occurs in CPD but it
would be really helpful to minimise it!! All suggestions
welcomed.

With best wishes

Keith Ryan
Plymouth Marine Laboratory
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel: ++44 1752 633294
Fax: ++44 1752 633102
e-mail: k.ryan-at-pml.ac.uk
PML web site: http://www.npm.ac.uk/pml







From: taylor-at-iris1.sb.fsu.edu (Kenneth A. Taylor)
Date: Tue, 20 Aug 1996 11:33:46 -0600
Subject: NIH Image 1.60

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I have been trying to average some color video pictures using NIH Image
running on a Macintosh 8500 using the built in video option. Input is from
a Sony 8 mm video camera running in VCR mode. The capture video picture
looks OK but when I try to grab the picture using "Average frames" option I
get an image with a bizarre lookup table. In B/W mode "Average frames"
seems to work OK. Is "Average frame" not possible for color input? Is the
built-in video option not supported or not supported for averaging frames?

Thanks for any help you can give me.

Cheers -- Ken Taylor

{ {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } {

Kenneth A. Taylor, Ph.D. Phone: 904-644-3357
Institute of Molecular Biophysics Fax: 904-561-1406
Florida State University E-mail: taylor-at-sb.fsu.edu
Tallahassee, FL 32306-3015
Home pages: http://www.sb.fsu.edu/~taylor/
http://www.fsu.edu/~biology/faculty/kat.html

{ {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } { {} } {








From: bergrh-at-msuvx2.memphis.edu (R. Howard Berg)
Date: Tue, 20 Aug 1996 10:42:49 -0600 (CST)
Subject: Re: freeze-sub - plant leaf tissue

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It sounds like the substitution is adequate; I'd bet you would need on the
order of several weeks infiltration time: the resin is of higher viscosity
at (-)25 and the rate of diffusion through the tissue is also much slower.
Try infiltrating in 100% resin for a month...

} Hello can anyone help me? I am having resin infiltration problems using
} freeze-substitution on leaf tissue from Myrothamnus flabellifolia.
}
} the freeze-substitution schedule used was:
}
} 1) samples were plunge frozen in
} liquid propane.
} 2) samples were then substituted in
} 100% methanol for 14 days at
} -82 C.
} 3) samples were then placed in a
} cryochamber and the temperature
} was ramped up from -80 C to -25 C over a 45 hr period.
} 4) samples were infiltrated in two
} changes of 100% LR Gold resin (+
} benzil) at -25 C with each change for a duration of 1 hr.
} 5) samples were then placed in 100% LR
} Gold (+benzil) at -25 C for
} 16 hr.
} 6) samples were then placed into
} another change of 100% LR Gold
} (+benzil) for 248 hr at -20 C.
} 7) samples were polymerized in a final
} change of 100% LR Gold (+
} benzil) under UV light at -25 C for 24 hr.
}
} The blocks polymerized fine but there seemed to be no resin infiltration
} into the tissue at all - the leaf was a spongy mass contained within the
} polymerized block of resin.
}
} Is this freeze-substitution schedule OK?
} Is this freeze-substitution schedule suitable for leaf tissue? Is there
} a tried freeze-substitution protocol for plant leaf tissue?
} Are there any ideas on enhancing resin infiltration into leaf tissue?
}
} thanks for your time, Michaela.


R. Howard Berg, Ph.D.
Department of Microbiology &
Molecular Cell Sciences
Campus Box 526041
University of Memphis
Memphis, TN, 38152-6041
E mail: bergrh-at-cc.memphis.edu
phone: 901-678-4449 fax: 901-678-4457







From: Greg Strout :      gstrout-at-uoknor.edu
Date: Mon, 19 Aug 1996 11:30:55 -0500
Subject: Re: SEM: Prep of Aquatic samples - topographic studies.

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Message-ID: {321896BF.315-at-obsnsrv1.bio.uoknor.edu}
Microscopy-at-Sparc5.Microscopy.Com

LINDSAY UNSWORTH wrote:
}
} Hello,
} I am trying to find info on the preparation of a pond water sample.
} I was wondering if critical point drying or standard preparation would be
} better, considering diverse microorganisms present(diatoms, filamentous
} bacteria, zooplankton, cyanobacteria, algae and protozoa). I've so far
} only been able to find general information on SEM applications and the
} problems of dealing with structural water, the info is also quite old.
} Any useful libraries with online resources would also be greatly
} appreciated.
} Thanks muchly, Lindsay.
Lindsay,

I don't quite understand what you mean by standard preparation. If you
have a sample with organisms which are soft bodied, you usually take a
smple and fix it in some way (Transeau's, Lugol's, for LM work, or GA,
PFA then OsO4 for EM work etc.), then dehydrate thru an ETOH gradient,
and critical point dry (CPD). If you are only interested in the hard
structures, for example Diatoms, then other specific methods without CPD
are avilable. You could avoid CPD if you use hexamethyldisilazane
(HMDS), but you would still have to employ some "standard" fixation
regime followed with dehydration, then exchange with HMDS. The
Osmium-thiocarbohydrazide (OTOTO) method has been shown to stabilize
fragile structures while at the same time making them electrically
conductive. A reference you may want to look at would be Scanning
Electron Microscopy: A Students Handbook by Postek et. al. available
thru Ladd Industries (USA). It has a large section containing specific
protocols for fixing a variety of organisms/samples.
=================================================
Greg Strout
Electron Microscopist, University Of Oklahoma
e-mail gstrout-at-ou.edu
=================================================




From: schooley-at-mcn.org (Caroline Schooley)
Date: Tue, 20 Aug 1996 16:11:20 -0700
Subject: Re: SEM: Prep of Aquatic samples - topographic studies.

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Message-Id: {199608202259.PAA10367-at-mail.mcn.org}
X-Sender: schooley-at-mail.mcn.org
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Subscribe microscopy. schooley-at-mcn.org

Caroline Schooley
Educational Outreach Coordinator, Microscopy Society of America
Box 117
Caspar, CA 95420
Phone/FAX (707)964-9460
Email schooley-at-mcn.org






From: bozzola-at-siu.edu (John J. Bozzola)
Date: Tue, 20 Aug 1996 11:18:58 -0600
Subject: Re: What's the EM Exam?

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X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
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On Aug 20, Dave King asked "What's the EM Exam?"
}
} As an industry employed microscopist, I'd be interested to know
} what the EM exam is that Kristin Becker asked about. Is it
} related to government employment?
}
The Certification Program of the Microscopy Society of America (MSA) has
been in existence for over 16 years. The purpose of the MSA Certification
Program is to provide a means of assuring employers that holders of the
certificate possess an acceptable level of technical skill in biological
electron microscopy. Such a credential may be useful for job
classification, for establishing salary level or potential for advancement,
for personal goals and possibly for future use in applying for new
positions. MSA provides the only certification of biological electron
microscopy technologists in the United States, Central and South America,
although similar programs exist in Europe and in Canada. Certification is
not for everyone, since the process is both labor and time intensive and
currently covers only biological electron microscopy. (The board is
looking into the certification of non-biologists).

A written examination, administered two times a year (December and June),
and proctored by an MSA member in the candidates' vicinity, is graded by
the Certification Board. This exam consists of one hundred objective items
covering: instrumentation, tissue processing, sectioning and staining,
special techniques as well as photography, general chemistry, safety and
history. A syllabus and reading list is provided in the application packet
and the minimum passing score is 70%. One is permitted two attempts at
this exam over a one year period. After a third failure, one must reapply
anew.

For the practical exam (also accepted twice a year), the applicant is
required to submit: stained grids containing sections of three different
types of non-pathological tissues, a trimmed block of each tissue, stained
thick sections taken from the blocks and at least 12 unmounted 8x10"
electron micrographs. The negatives are also submitted along with details
of the protocol followed and figure descriptions. The practical exam is
graded by 2-3 members of the Certification Board and is permanently kept by
MSA as part of the applicant's file. In the event of failure, one may
resubmit a second practical within a one year period. A grading sheet
detailing criteria used to evaluate the practical exam is available from
the Certification Board.

The Certification Office has been contacted in the past by several state
civil service boards about the procedures involved in certification of
EMT's and some action at the state level for certification of technologists
is anticipated in the near future.

FOR MORE INFORMATION: phone MSA at 800-538-3672 or check the WWW at:
http://www.msa.microscopy.com/




####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: Greg Strout :      gstrout-at-uoknor.edu
Date: Mon, 19 Aug 1996 11:26:22 -0500
Subject: Re: freeze-sub - plant leaf tissue

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Message-ID: {321895AE.57CF-at-obsnsrv1.bio.uoknor.edu}

Michaela,
We also have encountered difficulty infiltrating freeze-substituted
plant material(pollen of Plumbago zeylanica). Our material was frozen
in liquid propane, substituted in 100% acetone at -90C for several days,
then warmed over an 8 hr. period to -25C, then to 4C, again taking
several hours.
Infiltration was performed at room temperature. The protocol used was a
standard (2:1 acetone:resin, 1:1, 1:2, 100% 3X) schedule. The resin was
cured at 52C (immunolabeling) for 12-14hrs. and was fine except for the
interior of the pollen grain where it was soft and tended to explode
when sectioned. We tried extending the infiltration schedule, using a
different transition solvent (acetone, free radicals, LR White :( not
good), and a variety of other things to no avail.
The bottom line? We tried placing the material from the final 100%
resin infiltration step into a mictowave oven and gave it two short
bursts lasting 6 sec each. The pollen grains sank to the bottom of the
conical centrifuge tube, we embedded the pollen and polymerized in a 52C
oven for 12 hrs. The material sectioned fine, no problems. We are
currently evaluating the pollen sections for immunolabelling
suitability.
Greg
Michaela wrote:
}
} Hello can anyone help me? I am having resin infiltration problems using
} freeze-substitution on leaf tissue from Myrothamnus flabellifolia.
}
} the freeze-substitution schedule used was:
}
} 1) samples were plunge frozen in liquid propane.
} 2) samples were then substituted in 100% methanol for 14 days at
} -82 C.
} 3) samples were then placed in a cryochamber and the temperature
} was ramped up from -80 C to -25 C over a 45 hr period.
} 4) samples were infiltrated in two changes of 100% LR Gold resin (+
} benzil) at -25 C with each change for a duration of 1 hr.
} 5) samples were then placed in 100% LR Gold (+benzil) at -25 C for
} 16 hr.
} 6) samples were then placed into another change of 100% LR Gold
} (+benzil) for 248 hr at -20 C.
} 7) samples were polymerized in a final change of 100% LR Gold (+
} benzil) under UV light at -25 C for 24 hr.
}
} The blocks polymerized fine but there seemed to be no resin infiltration
} into the tissue at all - the leaf was a spongy mass contained within the
} polymerized block of resin.
}
} Is this freeze-substitution schedule OK?
} Is this freeze-substitution schedule suitable for leaf tissue? Is there
} a tried freeze-substitution protocol for plant leaf tissue?
} Are there any ideas on enhancing resin infiltration into leaf tissue?
}
} thanks for your time, Michaela.




From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Wed, 21 Aug 1996 08:27:40 +0000
Subject: Variable pressure/Environmental SEM

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Message-Id: {s21ac84e.034-at-wpo.nerc.ac.uk}
X-Mailer: Novell GroupWise 4.1

Dear All

As the manager of some ageing EM equipment, ever
hopeful of funding etc., I would appreciate any comments on
the usefulness or otherwise of the type SEM's which offer
'poor' vacuum in the specimen chamber so that wet/fresh
specimens can be examined.

Our problem is that we do marine biology and most
specimens come with a layer of salt water or, if dissected,
then a film of body fluid. What happens to the surface layer -
I know from cryo that after sublimation we are left with a
driedsalt layer which can be unhelpful! Sometimes I have
been known to rinse specimens in fresh water - that helps!

Any comments would be welcomed


With best wishes

Keith Ryan
Plymouth Marine Laboratory
Citadel Hill
Plymouth Devon PL1 2PB
England

Tel: ++44 1752 633294
Fax: ++44 1752 633102
e-mail: k.ryan-at-pml.ac.uk
PML web site: http://www.npm.ac.uk/pml






From: gbza40-at-udcf.gla.ac.uk
Date: Wed, 21 Aug 1996 09:41:22 +0100 (BST)
Subject: Re: SEM & ruptured cells

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Keith

Have you access to a low temp/high vac freezedryer ? If so our best preps
of cultured cells (long traction fibres, delicate surface cell-cell contact
projections and so on) have been produced from plunge freezing cells on 10
mm thin glass coverslips after fixing following a TEM protocol including
glutaraldehyde/osmium/uranyl acetate stages. The plunging has to be from
distilled water of course but as these cells are stabilised by the multifix
sequence they're no longer osmotically sensitive. An overnight sublimation
at -80C with a 10C/hr warm up the next day provides good results. The proof
of the pudding is in the images (?!) obtained compared to CPD where the much
greatear shrinkage due to extraction can devastate the structure (whether or
not you take precautions to avoid water in the CO2 supply).

Good Luck

Laurence Tetley





} Hello all
}
} We have a problem with settled phagocytic blood cells from
} the mussel (Mollusca: Lammellibranch: Mytilus) which
} are ruptured after critical point drying . The cells have very
} thin, delicate sheets of cytoplasm around the nuclear area
} which almost merge into the background. These split but so
} too can the thicker nuclear area.
}
} The cells are settled onto glass cover slips, fed bacteria,
} fixed in glutaraldehyde, dehydrated with acetones (typically
} 30, 50, 70, 90 and 100%) and then go into liquid carbon
} dioxide for drying. I know shrinkage occurs in CPD but it
} would be really helpful to minimise it!! All suggestions
} welcomed.
}
} With best wishes
}
} Keith Ryan
} Plymouth Marine Laboratory
} Citadel Hill
} Plymouth
} Devon PL1 2PB
} England
}
} Tel: ++44 1752 633294
} Fax: ++44 1752 633102
} e-mail: k.ryan-at-pml.ac.uk
} PML web site: http://www.npm.ac.uk/pml
}
}
}
}
}
Dr Laurence Tetley
IBLS EM Centre
Joseph Black Building
University of Glasgow
Glasgow G12 8QQ

email gbza40-at-udcf.gla.ac.uk
tel. 0141 330 4431
fax 0141 307 8016





From: shsiriw-at-slvaxa.umsl.edu (Haresh Siriwardane)
Date: Wed, 21 Aug 1996 07:45:02 -0500
Subject: Unsubscribe

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From: zaluzec-at-sparc5.microscopy.com (Nestor J. Zaluzec)
Date: Wed, 21 Aug 1996 08:03:01 -0500
Subject: Announcement of Image-Pro Listserver

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Message-Id: {199608211257.HAA04023-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
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Colleagues....

I was asked to review this announcement as to the appropriateness of
posting on the Microscopy Listserver. Since it is a public & free listserver
dedicated to answering questions about a program that many
Microscopists use, I have agreeded to allow the posting. Thanks
to Media Cybernetics for clearing it with me first.

Nestor

Your Friendly Neighborhood SysOp
===========================================================================
}
} Nestor - Here is the announcement we discussed last week regarding our new
} email listserver. We would greatly appreciate it if you could forward this to
} the
} microscopy list.
}
} Thanks for your assistance -
}
} Scott Ireland
} Media Cybernetics, LP
} scott-at-mediacy.com
} http://www.mediacy.com
}
} _____________________________-
} }
} } An Image-Pro Plus users automated email listserver is now open to
} } subscribers!
} }
} } The imagepro-users mail listserver was created by Media Cybernetics to
} } provide
} } Image-Pro Plus users with an easy way of communicating with each other
} } world-wide.
} } The list should make it easier to find out about IPP solutions that others
} } may have
} } discovered and learn about what other IPP users are accomplishing.
} }
} } Once subscribed, members have the ability to send to the list - that
} } is, when you send an email to {imagepro-users-at-mediacy.com} , you are
} } sending to everyone who has subscribed. When anyone else who is subscribed
} } sends
} } to the list, members all receive a copy of the email.
} }
} } The imagepro-users mail list is a Majordomo automated list. Please send
} } questions or comments regarding this list to
} } {imagepro-users-owner-at-mediacy.com} .
} }
} } To subcribe, either:
} }
} } 1. Go to Web page {http://www.mediacy.com/ipplist.htm}
} }
} } *or*
} }
} } 2. Send email to {imagepro-users-request-at-mediacy.com} with the word
} } subscribe, on a line by itself, as the body of the message. It does not
} } matter what is put in the Subject field.
}
}






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Wed, 21 Aug 1996 08:53:41 -0400
Subject: Re: SEM & ruptured cells

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Perhaps an osmication step mught harden the tissue or use Parducz's Fixative
which is 6 parts 2% Os tetroxide and 1 part saturated mercuric chloride.
Take all prcautions as with osmium.
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
} Hello all
}
} We have a problem with settled phagocytic blood cells from
} the mussel (Mollusca: Lammellibranch: Mytilus) which
} are ruptured after critical point drying . The cells have very
} thin, delicate sheets of cytoplasm around the nuclear area
} which almost merge into the background. These split but so
} too can the thicker nuclear area.
}
} The cells are settled onto glass cover slips, fed bacteria,
} fixed in glutaraldehyde, dehydrated with acetones (typically
} 30, 50, 70, 90 and 100%) and then go into liquid carbon
} dioxide for drying. I know shrinkage occurs in CPD but it
} would be really helpful to minimise it!! All suggestions
} welcomed.
}
} With best wishes
}
} Keith Ryan
} Plymouth Marine Laboratory
} Citadel Hill
} Plymouth
} Devon PL1 2PB
} England
}
} Tel: ++44 1752 633294
} Fax: ++44 1752 633102
} e-mail: k.ryan-at-pml.ac.uk
} PML web site: http://www.npm.ac.uk/pml
}
}
}
}
}
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: Dave King (607)757-1248 T37/257-3 Ext DEKING-at-VNET.IBM.COM
Date: 21 Aug 1996 13:05:41 EDT
Subject: EM Exam

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Message-Id: {199608211659.LAA04531-at-Sparc5.Microscopy.Com}

To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}


Wow, what a response. Thanks very much for all of you who
explained what the EM Exam is. I'm sure there'll be plenty of
communication if it's expanded to include SEM and non-biological
work.

. {
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Wed, 21 Aug 1996 11:27:23 -0400 (EDT)
Subject: shrimp eyes

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I was wondering if anyone might have a good technique for the embedment
of shrimp eyes. I seem to have an infiltration problem even with vacuum
infiltration. I am using a 3% paraform.-2% glut in 0.1M cacodylate,
pH7.3 for the fix. Is there a better fix to use for the preservation of
ultrastructure? I have been leaving the eyes under vacuum all day before
I embed. It seems like the eyes are difficult to infiltrate with the
embedding resin. Do I have to remove the outer layer of the eye to
get better infiltation and what would be the best route to take for this
procedure? I appreciate any help available. Thanks.

Peace,

Phil Rutledge 8-{)




From: Susan Carbyn (Paula Allan-Wojtas) :      AllanWojtasP-at-em.agr.ca
Date: Wed, 21 Aug 1996 12:35:42 -0400
Subject: microwave problem

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Message-Id: {s21b02cc.095-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

I am having a problem with our Laboratory Microwave Oven, and was
hoping that someone may be able to shed some light on this problem.
We have a EMS-820 Precision Pulsed Lab. , 115V microwave oven, and
it keeps blowing fuses of 250W 15Amp. I set the effect to 100% and a
temperature restriction of 37 degrees celcius for the magnetron warm
up time at 2 minutes. This may be the problem right off the bat, but I am
not sure if this effect should cause a fuse in the microwave to blow.
Any comments or suggestions would be greatly appreciated!
Also, since I am doing some technique development, does anyone have
any comments, results or comparisons, on microwaving in hot spots as
opposed to cold spots.
My email address is carbyns-at-em.agr.ca
Thanks in advance,
Susan





From: Gillian Bond :      gbond-at-nmt.edu
Date: Wed, 21 Aug 1996 14:45:57 -0600 (MDT)
Subject: Graphite coating of TEM beam-deflector parts

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Hi everyone!

We are in need of help on a repair problem. We have some beam-deflector
moving parts which have become "sticky" due to partial loss of their
graphite lubricant coating. Can anyone advise us on how to recoat them
with graphite? Many thanks for your time.

Gill Bond
Dept. of Materials & Met. Eng.
New Mexico Tech






From: versaci raul :      versaci-at-cnea.edu.ar
Date: Wed, 21 Aug 1996 15:01:55 -0700
Subject: I am sorry.

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Message-ID: {321B8753.3765-at-cnea.edu.ar}

Dear all,
I am sorry about JEOL after.

R. Versaci




From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Wed, 21 Aug 1996 13:02:32 -0500
Subject: Re: SEM & ruptured cells

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Message-Id: {199608211756.MAA13953-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Another thing to try is drying from HMDS (hexamethyldisilizane,
also as a tetramethyletc.). I've found that this can work very well with
delicate cells. After the last 100% EtOH (which I prefer to acetone),
transfer to HMDS by which ever of the following works best for you guys:
1:1 EtOH (or acetone): HMDS
-or- \
2:1 \
1:2 \
-or- } all times = time for each change in 100% EtOH (acetone)
3:1 /
1:1 /
1:3 \
then 3 X 100% HMDS
evaporate dry from last step at either room temp or 45-60 degrees
Celsius.
The evaporation temp seems to have the greatest affect on
morphology, once the infiltration is good enough for complete replacement
of the dehydrating fluid with HMDS. That is, you can "under infiltrate"
(leaving some EtOH or whatever behind), but it's harder to "over
infiltrate".
Phil
} Perhaps an osmication step mught harden the tissue or use Parducz's Fixative
} which is 6 parts 2% Os tetroxide and 1 part saturated mercuric chloride.
} Take all prcautions as with osmium.
} } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } } }
} } Hello all
} }
} } We have a problem with settled phagocytic blood cells from
} } the mussel (Mollusca: Lammellibranch: Mytilus) which
} } are ruptured after critical point drying . The cells have very
} } thin, delicate sheets of cytoplasm around the nuclear area
} } which almost merge into the background. These split but so
} } too can the thicker nuclear area.
} }
} } The cells are settled onto glass cover slips, fed bacteria,
} } fixed in glutaraldehyde, dehydrated with acetones (typically
} } 30, 50, 70, 90 and 100%) and then go into liquid carbon
} } dioxide for drying. I know shrinkage occurs in CPD but it
} } would be really helpful to minimise it!! All suggestions
} } welcomed.
} }
} } With best wishes
} }
} } Keith Ryan
} Greg Erdos

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu

*********** looking for a job again ***********






From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 21 Aug 1996 13:58:50 -0400 (EDT)
Subject: Re: Variable pressure/Environmental SEM

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} As the manager of some ageing EM equipment, ever
} hopeful of funding etc., I would appreciate any comments on
} the usefulness or otherwise of the type SEM's which offer
} 'poor' vacuum in the specimen chamber so that wet/fresh
} specimens can be examined.
}
} Our problem is that we do marine biology and most
} specimens come with a layer of salt water or, if dissected,
} then a film of body fluid. What happens to the surface layer -
} I know from cryo that after sublimation we are left with a
} driedsalt layer which can be unhelpful! Sometimes I have
} been known to rinse specimens in fresh water - that helps!
}
} Any comments would be welcomed
}
Dear Keith,
I have not had experience with ESEM's, but I have used an environ-
mental chamber on the high-voltage TEM. I don't know what is commercially
available, but I can tell you a few relevant details about our system.
Ours is a differentially-pumped chamber, which has a reservoir of
water (or, potentially, other liquids) and four apertures. The inner two
separate the inner chamber from the differential pumping region, and the
outer two separate that region from the high vacuum of the column. Water
vapor flows through the inner pair of apertures and is pumped away, and the
beam passes through all four apertures. The aperture size was selected to
allow steady-state operation--water from the reservoir evaporates at the
same rate as vapor flows out--and the pressure in the chamber is the vapor
pressure of water at the operating temperature.
The chamber stays at ~100% humidity; we tested this by finding dif-
fraction patterns from catalase, which is irreversably disordered at { 95%
humidity. We also cultured some PTK1 cells on gold grids, removed them
from the dish, rinsed them with fresh water, blotted the excess until only
a thin film of water remained (~10 A) and put them into the chamber.
Some were left in the chamber for 1/2 hour and then removed and
replaced into the culture dish without being exposed to the beam, and
others were similarly left in the chamber then subjected to the beam and
photographed. Only one edge of these grids was exposed to the beam, but
there was x-ray exposure to the entire grid. Photos were taken with Lo
Dose film, so there was less exposure than for the usual imaging conditions.
Subsequently, these grids were also replaced into a culture dish. At least
some of the cells on each of the grids were still alive after these treat-
ments--of course, none of the irradiated cells on the exposed edges of the
grids survived.
The answer to where the water on your specimens would go is that at
steady state it should neither decrease from evaporation nor increase from
condensation, if the vapor pressure at the reservoir is the same at that
at the specimen. This may mean that you would have to add a solute to the
reservoir to achieve the same vapor pressure as sea water. Your specimens
may also be less tolerant of a fresh-water rinse. Good luck.
Yours,
Bill Tivol




From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Tue, 20 Aug 1996 17:21:07 +0000
Subject: SEM & ruptured cells

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In response to:
----- Begin Included Message -----


We have a problem with settled phagocytic blood cells from
the mussel (Mollusca: Lammellibranch: Mytilus) which
are ruptured after critical point drying . The cells have very
thin, delicate sheets of cytoplasm around the nuclear area
which almost merge into the background. These split but so
too can the thicker nuclear area.

The cells are settled onto glass cover slips, fed bacteria,
fixed in glutaraldehyde, dehydrated with acetones (typically
30, 50, 70, 90 and 100%) and then go into liquid carbon
dioxide for drying. I know shrinkage occurs in CPD but it
would be really helpful to minimise it!! All suggestions
welcomed.

With best wishes

Keith Ryan
Plymouth Marine Laboratory
Citadel Hill
Plymouth
Devon PL1 2PB
England

Tel: ++44 1752 633294
Fax: ++44 1752 633102
e-mail: k.ryan-at-pml.ac.uk
PML web site: http://www.npm.ac.uk/pml

----- End Included Message -----


Keith, some time ago I surveyed and tried to address the shrinkage problem.
The best suggestions I came across were to use mordants (tannic acid, uranyl,
TCH-osmium, to fortify
the cells against shrinkage and to be sure the final 100% solvent dehydration
is anhydrous. There were a few interesting papers you might look into:

D. Schroeter etal, A Procedure for Rupture free Preparation of Confluently
Grown Monolayer Cells for SEM. Jou of Elec. Miscroc. Techn. 1:219-225 (1984)

K-R Peters & R. Pohl, Freeze Substitution of Chemically Stabilized Samples for
Biological Field Emission SEM. Microsc. Res & Techn. 22:170-184 (1992)

H. Gamliel, Optimum fixation conditions may allow air drying of soft biological
specimens with minimal cell shrinkage and maximum preservation of surface
features. Scanning Elect. Microsc. 1985; IV: 1649-1662.

L. Wollweber, etal. The use of a simple method to avoid cell shrinkage during SEM preparation. J Microsc. 121 (2) 185-189 (1981)

good luck

Ed Basgall, PhD
Dept of Chem
Penn State Univ.
University Park, PA 16802




From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Thu, 22 Aug 1996 11:08:15 +1100
Subject: Re: shrimp eyes

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X-Sender: diana-at-pc-0.eye.usyd.edu.au
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} I was wondering if anyone might have a good technique for the embedment
} of shrimp eyes.

I deal with rather bigger eyes here; the smallest I've had are mice. There
is absolutely no way that any whole intact eye that I've come across can be
effectively fixed or embedded. Shrimp eyes, though smaller, may be the
same. When you consider the structure of the outer layers, the eye is
obviously designed to for toughness and impermeability. Even the outer
layers of a cut up eye are hard to embed nicely. The best way to fix/embed
whole eyes is to make a slit across the anterior chamber and allow the
fixative etc to get to the tissue from the inside. Obviously, continuous
agitation is needed to allow the chemicals in and out. If you don't need
the eye intact, dissect out the tissue of interest. In particular, throw
away the outer collagenous layers if you don't need them and it will all be
a lot easier. Oh, I use 2.5% glut + paraf. in cacodylate and have for
years. Let me know if you need any help.

Diana van Driel
Dept Ophthalmology
Sydney University C09
AUSTRALIA 2006






From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: 22 Aug 96
Subject: Certifcation issues

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X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc04

-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #3.0 ] --

There is another approach and that is to accredit the laboratory for the
work that is actually being done rather than to certify individuals.
This might in fact be the only path possible presently for those doing
things other than life science TEM work.

The organization currently accrediting microscopy laboratories to the
standard of ISO Guide 25 is the American Association for Laboratory
Accreditation (A2LA). You can get to their website via the SPI home
page, clicking on "Other WWW Sites of Interest to Microscopy and
Microanalysis People" (toward the bottom of the page).

There are two other sites of interest to microscopy people that are
linked to that same page:

ALMA (Analytical Laboratory Managers Association) and
ACIL (American Council of Independent Laboratories.

With all of the concern about laboratory financing, and the "business"
aspects of running a major microscopy facility, I have found ALMA
membership especially valuable. It is great for networking with other
laboratory managers from all over the USA and some from even outside the
USA.

ACIL is the main organization representing for-profit tax-paying
independent analytical and testing laboratories and they have their own
set of programs to benefit such laboratories. It represents a fantastic
opportunity for networking for anyone offering laboratory services as a
business.

If the issue is really one of quality, and the ability to be recognized
for such quality, one might do well to investigate the A2LA
accreditation program for their laboratory.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.
com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.
com


Look for us!
############################
WWW: http://www.2spi.com
############################
=====================================================




From: manuela :      manuelap-at-petermac.unimelb.edu.au
Date: Thu, 22 Aug 1996 17:11:13 +1000
Subject: tem:grids

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Is there a supplier (other than Alltech) who stock grids that are marked in
some way i.e. with numbers or letter? Preferably hexagonal and 200 or 300 mesh.

Thanks in advance

Manuela





From: Dr. Molnar Peter :      molnarp-at-lib.dote.hu
Date: Thu, 22 Aug 1996 10:37:14 +100
Subject: Nikon-Lucia system

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Hi Everybody:
I would appreciate some input from anybody with the experience about
Nikon's image analysis and morphometric system named "Lucia". The
representatives of the system are here today at our Department and
would want to convince us that it is worth buying their system. I
know that all little bugs and inconveniences only appear during
staedy use of a software like that. In addition, they are willing to
sell the software only with their hardware and that gives me second
thougths
Is there anybody out there who has already tried this software?
Please advise me and be critical.
Thanks in advance
Peter P. Molnar
e-mail: molnarp-at-lib.dote.hu




From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Thu, 22 Aug 1996 08:40:34 -0400 (EDT)
Subject: Re: microwave problem

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Dear Susan,

Call EMS about the microwave fuse problem. They should be more than
willing to help out.
As to the hot\cold spots, I would use the cold, to "cool" areas and
not the hot spots.
Obtain a copy of Kok and Boon's Microwave Cookbook, this is a very
good reference.

Best of Luck,
Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu




From: Gary Login :      glogin-at-bih.harvard.edu
Date: Thu, 22 Aug 1996 08:58:44 -0400
Subject: Re: microwave problem

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Message-Id: {v03007800ae4200ad7108-at-[134.174.111.7]}
In-Reply-To: {s21b02cc.095-at-EM.AGR.CA}
Mime-Version: 1.0
Content-Type: text/enriched; charset="us-ascii"

Regarding Susan's inquiry:


} I set the effect to 100% and a

} temperature restriction of 37 degrees celcius for the magnetron warm

} up time at 2 minutes. I am

} not sure if this effect should cause a fuse in the microwave to blow.


} Any comments or suggestions would be greatly appreciated!


If a microwave oven's power is on while it is unloaded (e.g., no water
load or missing its glass tray) than a safety fuse may open (i.e.,
blow) in order to protect the magnetron from overheating and damage.



} Also, since I am doing some technique development, does anyone have

} any comments, results or comparisons, on microwaving in hot spots as

} opposed to cold spots.


The answer to the question about "hot and cold spots" is specific to
the sample and the goal of the procedure (i.e., staining, fixation, or
embedding). I recently reviewed this issue in
{fontfamily} {param} Geneva {/param} G.R. Login, N. Tanda and A.M. Dvorak,
"Calibrating and standardizing microwave ovens for
microwave-accelerated specimen preparation. A review.," Cell Vision,
3, 172-179 (1996). {/fontfamily} For example, the advantages of fixing
tissues for LM or EM in "hot spots" in the microwave oven are increased
speed of specimen handling and decreased exposure time to hot immersion
solutions. (Fixing tissues in "cold spots" extends their dwelling time
in hot fixatives which may superimpose conductive heating artefacts on
the tissue and decrease antigen immunoreactivity- reviewed
in {bold} {bigger} {/bigger} {/bold} G.R. Login and A.M. Dvorak, "Methods
of microwave fixation for microscopy. A review of research and clinical
applications: 1970-1992," {italic} Prog Histochem Cytochem, {/italic}
{bold} 27/4, {/bold} 1-127 (1994).


I would be happy to advise you on your application- please send me a
separate e-mail.




Dr. Gary R. Login

Dept. Pathology

Beth Israel Hospital

330 Brookline Avenue

Boston, MA 02215


phone: 617-667-2034

fax: 617-667-8676


e-mail: glogin-at-bih.harvard.edu





From: Damian_Neuberger_at_RLT011-at-ccmailgw.mcgawpark.baxter.com
Date: Thu, 22 Aug 1996 08:54:49 -0500
Subject: HMDS method

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Hi everyone. I was just migrated to cc:Mail and still learning how to
use it which explains why I mistakenly deleted Phil Oshel's recent
message on drying with HMDS. I was in the process of working out a
better method of transfer from ethanol to HMDS and would really
appreciate it if someone would be good enough to send a copy to me at
neuberd-at-baxter.com. Thanks for your help.

Damian Neuberger
Baxter Healthcare, Corp.





From: scott.wight-at-nist.gov (Scott Wight)
Date: Thu, 22 Aug 1996 09:10:02 -0500
Subject: metal evaporation mask

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Microscopy listserve readers:
Is anyone aware of a company or vendor that makes or sells masks which are
placed on substrates (ie:carbon planchet) before metal evaporation (ie:Au,
Al) which leave a grid structure in the evaporated metal on the substrate?
Size would be 2mm square to 12 mm square. If you can suggest a supplier or
any leads on a manufacturer please contact me at scott.wight-at-nist.gov
Thanks, Scott

Scott Wight e-mail: SCOTT.WIGHT-at-NIST.GOV
NIST - Microanalysis Group W voice: 301-975-3949
Bld 222, Rm A113 | fax: 301-216-1134 or 301-417-1321
Gaithersburg, MD 20899 \|/ disclaimer:
Any opinion expressed is my own and does not represent those of my employer.
Web Page: http://www-sims.nist.gov/Division/MicroGroup.html






From: Campbell36-at-aol.com
Date: Thu, 22 Aug 1996 14:31:03 -0400
Subject: Re: metal evaporation mask

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In a message dated 96-08-22 13:48:00 EDT, scott.wight-at-nist.gov (Scott Wight)
writes:

{ { Subj: metal evaporation mask
Date: 96-08-22 13:48:00 EDT
From: scott.wight-at-nist.gov (Scott Wight)
To: microscopy-at-Sparc5.Microscopy.Com

Microscopy listserve readers:
Is anyone aware of a company or vendor that makes or sells masks which are
placed on substrates (ie:carbon planchet) before metal evaporation (ie:Au,
Al) which leave a grid structure in the evaporated metal on the substrate?
Size would be 2mm square to 12 mm square. If you can suggest a supplier or
any leads on a manufacturer please contact me at scott.wight-at-nist.gov
Thanks, Scott } }

Scott, We use Towne Technologies, in Somerville, New Jersey (908-722-9500)
for the fabrication of our masks for CV-Dot systems.

James Campbell
Marketing Manager
Denton Vacuum, Inc
609-439-9100 Fax 609-439-9111
j_campbell-at-dentonvacuum.com
www.dentonvacuum.com
August 22, 1996
2:25 pm




From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Thu, 22 Aug 1996 12:36:33 -0800
Subject: belljar for varian ve-10

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Howdy all

anyone have a bell jar to fit a varian VE-10 vacuum evaporator they are
willing to part with?

steve

---------------------------------------------------------------------

Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Dr.
San Diego, CA 92182-4614
phone: (619)594-4523
fax:(619)594-5676
email:sbarlow-at-sunstroke.sdsu.edu






From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Thu, 22 Aug 1996 12:38:58 -0800
Subject: Javelin light microscope

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Message-Id: {v02110103ae4275563a99-at-[130.191.238.141]}
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howdy all

anyone know where I can get some information on a Javelin light microscope?
My user didn't know if this was the model name or manufacturer name.

steve

---------------------------------------------------------------------

Dr. Steven Barlow
EM Facility/Biology Dept.
San Diego State University
5500 Campanile Dr.
San Diego, CA 92182-4614
phone: (619)594-4523
fax:(619)594-5676
email:sbarlow-at-sunstroke.sdsu.edu






From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 22 Aug 1996 15:02:29 -0400 (EDT)
Subject: Re: Variable pressure/Environmental SEM

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}
} Bill
} Would you not expect a considerable temperature increase where your
} beam is, and therefore a (regional) RH of far less than 100%?
}
Dear Stephan,
Yes, there is probably a considerable local temperature increase,
but this is minimized by the use of low-dose imaging. The local water
vapor pressure probably doesn't change too much, since the water-vapor
currents will keep the pressure uniform. One thing to remember is that
there is no air--only water--in the set-up we use.
Under these conditions, there is neither net condensation nor evap-
oration, so the thin aqueous film on wet specimens stays the same. The
catalase ED shows that the order is preserved for at least 100 sec--the
length of the exposure of the film--since the pattern is still visible
after the picture is taken.
Yours,
Bill Tivol




From: MalHillmn-at-aol.com
Date: Thu, 22 Aug 1996 19:16:16 -0400
Subject: Employment Opportunities!

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I am a recruiter with the firm of Michigan Search Plus in Farmington Hills,
MI.

I am seeking candidates per the following requirements:

Sales Persons are being sought by a highly regarded manufacturer and
international marketer of high value fluorescence microscopy imaging
equipment, which are being sold into leading edge life science research
facilities (public and private). Requires life science background and sales
experience. Compensation package $100K +/- (base & commission & bonus), plus
benefits and relocation assistance. Multiple locations in the U.S. Involves
50%+ travel.

Programmers are being sought for a manufacturer and international marketer of
high value fluorescence microscopy imaging equipment. Instruments consist of
analog and digital components and uses extensive data base to generate 2 &
3-D images, conduct kinetic studies, etc. Requires 2+ years of programming
experience with Windows, C++, objective oriented design and Microsoft
Foundation Classes. Compensation $55K +/-, plus full benefits and relocation
assistance.

ALL INQUIRIES WILL BE HANDLED WITH UTMOST CONFIDENTIALITY AND AT NO COST TO
CANDIDATES!

Interested candidates can contact Mal Hillman, Michigan Search Plus, 25882
Orchard Lake Road, Suite 207, Farmington Hills, MI 48336; Fax to:
810-471-6572.

Please post, e-mail, etc, this message as you deem fit.

Sincerely,
Mal Hillman, Ph.D.
Consultant




From: Leo Marin :      leo-at-spine.med.utoronto.ca (by way of zaluzec-at-microscopy.com (Nestor J. Zaluzec))
Date: Thu, 22 Aug 1996 18:51:28 -0500
Subject: curling of bio. specimens during EM processing

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Is there any way of controlling or preventing specimen curling during
processing for TEM? My tissue (Drosophila larva) is about 1mm wide by
3-4mm long by 1/4 mm thick. Most of the curling takes place during
ethanol dehydration, especially from 70%.
Your suggestions will be greatly appreciated.

Thanks

Leo







From: MATTHEW JOHNSTON :      n1414194-at-sparrow.qut.edu.au
Date: Fri, 23 Aug 1996 10:47:32 +1000 (EST)
Subject: Subscribe to Microscopy

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Subscribe Microscopy {M.Johnston-at-student.qut.edu.au}






From: Gary Login :      glogin-at-bih.harvard.edu
Date: Thu, 22 Aug 1996 15:23:59 -0400
Subject: "Hot and Cold spots" in a Microwave Oven

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Message-Id: {v03007802ae425309aebc-at-[134.174.111.7]}
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Comment on the Definition of "hot and cold spots" in a microwave oven.

These are jargon terms and they are misleading. I apologize for any
confusion I caused by using these terms in an e-mail message I sent earlier
today.

"Hot and cold spots" loosely refer to the varying electric field pattern of
microwave energy in a microwave oven. "Hot spots" have a measurably higher
field than "cold spots". Although, biological samples can heat faster in
higher electric fields, samples will be Heated in "hot and in cold spots"!

Knowing the location of nonuniform fields is of practical importance for
reproducibly heating samples. Nonuniform fields are present in ALL
household and laboratory microwave ovens (even ovens with turn tables)!!

Using a Neon Bulb Array (available from several distributors of microscopy
products), you can visualize the location of hot and cold spots in any
microwave oven. Other tools such as Agar Saline-Giemsa Blocks are useful
for predicting nonuniform heating patterns within a biological sample.
Using these tools, intelligent decisions can be made for reproducible
sample placement within a microwave oven.

References are available upon request.





Dr. Gary R. Login
Dept. Pathology
Beth Israel Hospital
330 Brookline Avenue
Boston, MA 02215

phone: 617-667-2034
fax: 617-667-8676

e-mail: glogin-at-bih.harvard.edu






From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Thu, 22 Aug 1996 17:53:57 -0700 (PDT)
Subject: Javelin Microscope?

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I have not heard of Javelin microscopes however we have a Javelin TV
camera used with a microscope.The manufacturer was Javelin Electronics in
Torrance, CA, USA, (800) 421-2716. Our vendor was Technical Instrument Co.
in San Francisco (415) 431-8231. Hope this helps.

Larry Ackerman mishot-at-itsa.ucsf.edu
The Laboratories of Lily & Yuh Nung Jan Voice (415) 476-8751
Howard Hughes Medical Institute
UCSF, Box 0724, Rm U426 FAX (415) 476-5774
533 Parnassus Ave.
San Francisco, CA 94143






From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Thu, 22 Aug 1996 15:35:32 -0500
Subject: Re: metal evaporation mask

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In message {v02140b00ae4218182db1-at-[129.6.98.75]} Scott Wight writes:
} Microscopy listserve readers:
} Is anyone aware of a company or vendor that makes or sells masks which are
} placed on substrates (ie:carbon planchet) before metal evaporation (ie:Au,
} Al) which leave a grid structure in the evaporated metal on the substrate?
} Size would be 2mm square to 12 mm square. If you can suggest a supplier or
} any leads on a manufacturer please contact me at scott.wight-at-nist.gov
} Thanks, Scott
}
} Scott Wight e-mail: SCOTT.WIGHT-at-NIST.GOV
} NIST - Microanalysis Group W voice: 301-975-3949
} Bld 222, Rm A113 | fax: 301-216-1134 or 301-417-1321
} Gaithersburg, MD 20899 \|/ disclaimer:
} Any opinion expressed is my own and does not represent those of my employer.
} Web Page: http://www-sims.nist.gov/Division/MicroGroup.html


Scott, Try Buckbee-Mears, 278 E. 7th st., St. Paul, MN. (612)228-6425,
228-6572FAX. Dave Finegan is East Coast representative. They make many kinds of
metal mesh that might work for you.

Happy hunting!

Gib


Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996
Over, but not forgotten, and it was a blast!





From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Fri, 23 Aug 1996 14:53:19 +1100
Subject: penetration of antibodies - glut fixed tissue

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Message-ID: {199608230344.WAA04570-at-IndyNet.indy.net}
To: Microscopy list {microscopy-at-Sparc5.Microscopy.Com} ,
"SCOTT.WIGHT-at-NIST.GOV" {scott.wight-at-nist.gov}

I label whole retinae (app 300 mic. thick) for immunoEM using various
antibodies. There is no problem getting penetration of the antibodies and
gold reagent into paraformaldehyde or PLP fixed tissue, but as soon as
glutaraldeyhde is introduced, penetration of the antibodies plummets. I can
get away with 0.1% glut, sometimes, but no higher. Does anyone know of a
trick to increase permeability without compromising ultrastructure. I
routinely use saponin and have tried alcohols and freeze/thaw.


Diana van Driel
Dept Ophthalmology
Sydney University C09
AUSTRALIA 2006






From: jflah-at-macollamh.ucd.ie
Date: Fri 23 Aug 1996 07:45:02 -0500
Subject: Unsubscribe

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Unsubscribe

Department of Botany, University College Dublin, Belfield, Dublin 4,
Ireland

E-mail: jflah-at-macollamh.ucd.ie




From: manuela :      manuelap-at-petermac.unimelb.edu.au
Date: Fri, 23 Aug 1996 16:15:06 +1000
Subject: TEM grids

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Thanks to everyone who replied. I think my request has been misunderstood!!
I am aware of the existence of finder grids etc. I'm actually after grids
which are marked individually for the purpose of identification of the grid
itself not as a reference point on the grid for the section. Alltech
supplies 400 mesh square grids marked A-Z for this purpose. However, we
would prefer 200-300 hex grids for the particular nature of the work we do.

Again thanks to everyone who replied as I continue with my quest!!!

Manuela





From: Robin Griffin :      rgriffin-at-eng.uab.edu
Date: Thu, 22 Aug 1996 16:15:00 -0500
Subject: video cameras

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Message-ID: {c=US%a=_%p=UAB%l=ENGEM0-960822211500Z-16494-at-engem0.eng.uab.edu}

What is an acceptable level of resolution for a black and white video
camera?




From: Robin Griffin :      rgriffin-at-eng.uab.edu
Date: Fri, 23 Aug 1996 07:54:32 -0500
Subject: Getting B/W images from optical microscope

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Message-ID: {c=US%a=_%p=UAB%l=ENGEM0-960823125432Z-16556-at-engem0.eng.uab.edu}

I recently sent a message asking about black and white camera
resolution. I don't think it was very clear so I am trying again!

I am trying to purchase a camera to import black and white images from
an optical microscope to a computer. I would like to take these images
and use them for image processing and for putting into reports.

Questions:

1) What is reasonable resolution. Salesmen are talking about 560 to
1100 lines resolution. I don't want to spend too much but also want the
camera to stand up to developments in the computer/printer field (at
least for a while!). Does anyone have experience?

2) What kind of board do I need to import into the computer. I know
there are many on the market but has anyone had good/bad experience with
a particular type/brand?

3) I'd also like to purchase a printer to make cheap, reasonably
decent image prints for students in laboratories. Any preferences?

Thanks,

RGRIFFIN-at-eng.uab.edu




From: Owen P. Mills :      opmills-at-mtu.edu
Date: Fri, 23 Aug 1996 09:54:46 -0400
Subject: SPM repairs

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Hello,

We are preparing to purchase a SPM and, as with our other electron optical
instruments, would like to handle maintenance and repairs in-house after the
warranty period. No particular vendor has been chosen yet but we have some
favorites. All things being equal, repair history and service may be the
deciding issue. Here's a few questions for those of you with SPM's.

1. What kinds of repairs have been necessary to your SPM instruments?

2. Are service contracts available and/or necessary?

3. What should we get from the vendor "up front" in the way of manuals,
schematics, etc? This question could also be stated as, looking back over
your shoulder, what should you have gotten when you purchased your SPM?

4. Any thing else we should be aware of??

Send replies to me directly, I'll make a summary available to anyone who is
interested.

Thanks in advance.

Owen
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: Susan E. Babcock :      BABCOCK-at-coeadm.engr.wisc.edu
Date: Fri, 23 Aug 1996 09:33:02 CST6CDT
Subject: Unsubscribe

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Unsubscribe

Susan E. Babcock
Assistant Professor
Materials Science and Engineering
608-263-5696
babcock-at-engr.wisc.edu

1500 Engineering Drive
Madison Wisconsin 53706
USA




From: JSmiley-at-nwu.edu (John Smiley)
Date: Fri, 23 Aug 1996 12:01:12 -0600
Subject: E.polishing of Co-Fe

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John F. Smiley, Ph.D. e-mail: jsmiley-at-nwu.edu
Dept. of Neurology phone: 312-908-8571
Northwestern University FAX: 312-908-8789
11-499 Searle
320 E.Superior
Chicago, IL 60611






From: Paula Allan-Wojtas :      AllanWojtasP-at-em.agr.ca
Date: Fri, 23 Aug 1996 11:33:40 -0400
Subject: TEM and SEM of berries

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X-Mailer: Novell GroupWise 4.1

Does anyone have a good protocol (or references) for conventional
fixation of blueberries for TEM and or SEM?

Paula.





From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Fri, 23 Aug 1996 08:35:33 -0400
Subject: Re: HMDS method

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Hi Damian. I keep an archive of much of the biologic and computer
related problems posted to the list with their respective replies. There is
a file which I think you will be interested in. Go to the web address listed
at the end of this message and click on the "Tips & Tricks" button. This
will take you to the main menu and you will see another link to "SEM
Techniques and Instrumentation" The fille is in there. If you do not have
web access or cannot get the info, please let me know and I wil get it to
you. Hope this helps.







At 08:54 AM 8/22/96 -0500, you wrote:
} Hi everyone. I was just migrated to cc:Mail and still learning how to
} use it which explains why I mistakenly deleted Phil Oshel's recent
} message on drying with HMDS. I was in the process of working out a
} better method of transfer from ethanol to HMDS and would really
} appreciate it if someone would be good enough to send a copy to me at
} neuberd-at-baxter.com. Thanks for your help.
}
} Damian Neuberger
} Baxter Healthcare, Corp.
}
}
}




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {

Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 352-392-1295
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "





From: levin-at-ecsuc.ctstateu.edu (Martin Levin)
Date: Fri, 23 Aug 1996 10:26:57 -0500
Subject: Ultrotome Nova problems

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I have an LKB Ultrotome Nova (not a SuperNova) that just started giving me
some problems. The manual specimen arm control only moves the arm about
one cm from the top position. It does not go down nearly far enough to
activate the knife retraction and alarm. If I push it down by hand, it
does reach the bottom of its range and the retraction and alarm are
activated. The automatic arm control seems to work fine and does go
through the entire range.

I've checked inside and there doesn't seem to be anything wrong. The arm
lifting band is fine. I might add that while I was away from the lab for a
few weeks, the university, in its wisdom, turned my air conditioning off at
nights and during the weeked. As a result the specimen arm (and other
parts) had extensive surface rust. I've removed all the rust that I
could see. Could there be some hidden rust causing this? Anything that I
might lubricate?

The Nova is used mainly for teaching and the course starts in about one
week, so I need to resolve this problem quickly. If I can't solve the
problem myself, I will have to call in a technician. Anyone have any
suggestions?

Marty Levin


Martin A. Levin
Department of Biology
Eastern Connecticut State University
Willimantic, CT 06226
Phone: (860)465-4324 FAX: (860)465-5213






From: bert menco :      bmenco-at-casbah.acns.nwu.edu
Date: Fri, 23 Aug 1996 14:22:54 -0500
Subject: Unsubscribe

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} Unsubscribe
}
bmenco-at-casbah.acns.nwu.edu
}

Dr. Bert Menco
Research Associate Professor
Associate Director EM Lab
Department of Neurobiology & Physiology
O.T. Hogan Hall
Northwestern University
Evanston, IL 60208-3520
USA

Phone: 1-748-491-2866/7057 (w), 1-748-491-5211 (fax), 1-748-864-7210 (h)
E-mail: bmenco-at-casbah.acns.nwu.edu






From: Woody.N.White-at-mcdermott.com
Date: 8/22/96 4:15 PM
Subject: video cameras

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That will depend on what YOU need. However if the video is NTSC,
then you are fixed at 525 horizontal lines. Of the 525, only about
500 are available for image. The horizontal resolution depends on the
bandwidth of the overall system. Low is typically in the area of 250.
A "good" NTSC system can go (I forget exact) about 500-600 lines
equiv.

Higher resolution video is available, but is not compatible with the
average monitor/VCR, etc.

Woody


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What is an acceptable level of resolution for a black and white video
camera?




From: Michael P. Goheen :      mgoheen-at-indyvax.iupui.edu
Date: Fri, 23 Aug 1996 16:07:07 -0500
Subject: Biological TEM Charges

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We are trying to find out where we stand with regards to technical charges
for preparation of samples for TEM. WE currently charge $250.00/ sample
this includes processing, cutting thicks and thins, staining, and taking 8
to 10 electron micrographs per sample.

If anyone is willing to tell us what they are charging for similar services
it would be a great help to us.

Thanks
M Goheen
mgoheen-at-indyvax.iupui.edu





From: Beverly E Maleeff
Date: 23 Aug 96 16:54:46 EDT
Subject: Re: TEM and SEM of berries

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Message-Id: {9608240052.AA0220-at-pho903.sbphrd.com}
To: Paula Allan-Wojtas {AllanWojtasP-at-em.agr.ca} ,
Microscopy {Microscopy-at-Sparc5.Microscopy.Com}
{Beverly_E_Maleeff-at-sbphrd.com}

Paula:
Here's a good reference, from work done many years ago when I was at the USDA:

Sapers, GM, Burgher, AM, Phillips, JG and Jones, SB, 1984. Effects of
freezing, thawing and cooking on the appearance of highbush blueberries. J.
Amer. Soc. Hort. Sci. 109(1): 112-117, 1984.

Hope this helps.

Regards,
Bev Maleeff
SmithKline Beecham Pharmaceuticals
Toxicology-US, UE 0462
709 Swedeland Road
King of Prussia, PA 19406
610/270-7987
610/270-7202 fax
Beverly_E_Maleeff-at-sbphrd.com





From: Woody.N.White-at-mcdermott.com
Date: 8/22/96 4:15 PM
Subject: video cameras

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Message-Id: {n1371310990.81369-at-macmail.lbl.gov}
rgriffin-at-eng.uab.edu, Woody.N.White-at-mcdermott.com
X-Mailer: Mail*Link SMTP/QM 3.0.0

Reply to: RE} } video cameras

} Of the 525, only about 500 are available for image
Or even less, say 485.
The usual "standard" way to digitize (i.e., frame-grab) NTSC is into an image
with 640x480 (square) pixels. Which is not to say that the camera resolution
achieves 640x480, but it cannot be better when digitized this way.
Mike
--------------------------------------
That will depend on what YOU need. However if the video is NTSC,
then you are fixed at 525 horizontal lines. Of the 525, only about
500 are available for image. The horizontal resolution depends on the
bandwidth of the overall system. Low is typically in the area of 250.
A "good" NTSC system can go (I forget exact) about 500-600 lines
equiv.

Higher resolution video is available, but is not compatible with the
average monitor/VCR, etc.

Woody


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What is an acceptable level of resolution for a black and white video
camera?






From: Leo Marin :      leo-at-spine.med.utoronto.ca (by way of zaluzec-at-microscopy.com
Date: Thu, 22 Aug 1996 18:51:28 -0500
Subject: curling of bio. specimens during EM processing

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Message-ID: {n1371305454.53107-at-sjdccd.cc.ca.us}

We have worked with mosquito larvae with the same results. The curling is due
to differential shrinkage in the EtOH. We have had to go to variable pressure
SEMs where the specimen can be looked at uncoated. One is still limited to a
short period of time until dehydration occurs. The real answer if one can
afford it is the true Environmental SEM. We did that, I mean tried it, and it
worked. Now if only we had the funds!! Before we tried all sorts of reagents
to minimize shrinakge i.e. tannic acid, etc. etc., but there was always
shrinkage except in the Electroscan SEM which uses a hydrated chamber. One
could also use cryo but there is also problems with that.
Good Luck,
Judy M.

Judy Murphy, PhD
Microscopy Technology Center
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5600
e-mail: murphy-at-sjdccd.cc.ca.us
program web page: http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html

_______________________________________________________________________________

Is there any way of controlling or preventing specimen curling during
processing for TEM? My tissue (Drosophila larva) is about 1mm wide by
3-4mm long by 1/4 mm thick. Most of the curling takes place during
ethanol dehydration, especially from 70%.
Your suggestions will be greatly appreciated.

Thanks

Leo




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From: Woody.N.White-at-mcdermott.com
Date: 8/23/96 7:54 AM
Subject: Getting B/W images from optical microscope

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______________________________ Reply Separator _________________________________


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I recently sent a message asking about black and white camera
resolution. I don't think it was very clear so I am trying again!

I am trying to purchase a camera to import black and white images from
an optical microscope to a computer. I would like to take these images
and use them for image processing and for putting into reports.

Questions:

1) What is reasonable resolution. Salesmen are talking about 560 to
1100 lines resolution. I don't want to spend too much but also want the
camera to stand up to developments in the computer/printer field (at
least for a while!). Does anyone have experience?

Do you need "video" or will "still" shots work? Video of
this resolution is non-standard and quite expensive. A
(direct) digital single frame still camera might be a
cheaper solution, particularly if you can live with a
resolution of 640x480 (x16 million colors if desired).
Woody


2) What kind of board do I need to import into the computer. I know
there are many on the market but has anyone had good/bad experience with
a particular type/brand?

For video resolutions mentioned, GOOD capture boards are
expensive - several thousand dollars. A digital still
camera can usually download directly to a high speed
serial port.

Not that I specifically reccommend them, but two
examples of high res. video capture boards are
Matrox and Data Translation.
Woody

3) I'd also like to purchase a printer to make cheap, reasonably
decent image prints for students in laboratories. Any preferences?

This is the really tricky one! For B/W images, with the
appropriate software, a 600 or 1200 dpi laserjet will do a
fair job (halftone) at a minimal cost per copy. For
"rough" color print, 360+dpi inkjets could be used ( I
don't reccommend). "Full tone" (8bit/256 shades of gry)
high res. printers are available and produce near (B/W)
photographic grade output. They cost 5 times (+) more than
the 600dpi laserjet and the cost per copy is higher due to
special "paper" requirements. The best color copy, should
one need it, is produced by a dye sublimation printer.
Copy is (almost) as good as photographic, but the printers
are expensive (2-10 thousand) and the cost per print is in
the realm of $3/print. Cheaper ones are very slow also.
Woody


Thanks,

RGRIFFIN-at-eng.uab.edu


Comments: Woody White, Electron Microscopist




From: generalmicro-at-ccinet.ab.ca (General Microdevices, Inc.)
Date: Sun, 25 Aug 1996 12:31:47 -0600
Subject: In-column FE-SEM imaging required.

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Message-ID: {199608241900.OAA19137-at-IndyNet.indy.net}
To: Microscopy list {microscopy-at-Sparc5.Microscopy.Com}


Dear Readers,

We are needing some EM imaging (cross-sections) done on a thin film sample
whose features are small enough to require an in-column type FE-SEM to get
good quality pictures. If you operate one of these microscopes and would be
able to take our sample, or if you know of someone with such a microscope
whom we might contact, please drop me a line at the email address below.
Thank you,


Regards,

Cam Sorlie

____________________________________________________________________________
Box 1932 Main Station T: 1 403 435 2167
Cameron Sorlie, President Edmonton, AB T5J 2P3 Canada F: 1 403 433 9376
General Microdevices, Inc. -----------------------------------------------
generalmicro-at-ccinet.ab.ca
______________________________________________________
Microtechnology products for science and industry






From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Mon, 26 Aug 1996 11:37:48 +1000 (EST)
Subject: Re: curling of bio. specimens during EM processing

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} Is there any way of controlling or preventing specimen curling during
} processing for TEM? My tissue (Drosophila larva) is about 1mm wide by
} 3-4mm long by 1/4 mm thick. Most of the curling takes place during
} ethanol dehydration, especially from 70%.
} Your suggestions will be greatly appreciated.
}
} Thanks
}
} Leo
}
} Try making a sandwich of the specimen after glutaraldehyde fixation, using
a solvent resistant large pore membrane in a swinnex filter assembly. I use
silver membranes from SPI for this purpose. Then use a syringe to pass the
different changes of solution through the sandwich. The pressure of the
membrane ought to be somewhat controllable or you can put an extra gasket or
two between the membranes to pack them apart a bit. I reckon this will
retard curling. Let me know how you go. I use the system for SEM CPD of
small filterable organisms.

mel dickson
}
}
}





From: das-at-rri.sari.ac.uk (Diane Slater)
Date: Mon, 26 Aug 1996 09:03:26 +0100
Subject: Re: curling of bio. specimens during EM processing

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subscribe microscopy das-at-rri.sari.ac.uk





From: Jose A. Thomazini :      jathomaz-at-fmrp.usp.br
Date: Mon, 26 Aug 1996 09:02:39 -0200
Subject: unsubscribe

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Message-Id: {3221844F.51CF-at-uhura.fmrp.usp.br}

unsubscribe, please.




From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Mon, 26 Aug 1996 11:55:53 -0400 (EDT)
Subject: Thumbs plus

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I have had several people ask me about where to get Thumbs plus, the
shareware program we use for filing thumbnails of images.

The software is from Cerius Software. We downloaded it via links from
ZDNets shareware review page. This is a great source of information,
reviews, and downloads of freeware and shareware. The shareware search
engine can be found at http://206.66.154.152/index.html.

DISCLAIMER: I have no financial stake in just about anything- including
Thumbs plus and Ziff Davis publishing.


I hope this information is useful
Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************





From: keller-at-boulder.nist.gov (Bob Keller)
Date: Mon, 26 Aug 1996 13:45:26 -0700
Subject: call for papers - Materials Research Society

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X-Nupop-Charset: English

===================================================

CALL FOR PAPERS

Materials Research Society 1997 Spring Meeting
March 31 - April 4, 1997
San Francisco, California

ABSTRACT DEADLINE: November 1, 1996

Symposium J: Materials Reliability in Microelectronics VII

The inexorable drive for increased integrated circuit functionality and
performance places growing demands on the metal and dielectric thin films
used in fabricating these circuits, as well as spurring demand for new
materials applications and processes. In addition to meeting performance
and manufacturability requirements, these materials and processes must
yield circuits that operate reliably for many years. Achieving these goals
requires a better understanding of the relationship of thin-film material
properties and manufacturing processes to reliability degradation mechanisms.

This symposium is a continuation of the series that seeks to foster
this understanding. The aim is to provide a forum to bring together
researchers from industry and universities to discuss fundamental mechanisms
and materials properties pertinent to materials reliability issues in
the manufacture of submicron integrated circuits.

Papers are solicited in the following and related areas:

+ Reliability of metallic thin films and interconnects
- Electromigration in lines, contacts, vias
- Stress relaxation and stress voiding
- Metal microstructure: grain growth, texture, precipitate formation
- Effects of microstructure on electromigration
- Intermetallic formation and diffusion barriers
- New interconnect metallizations/novel alloys; alternative dielectrics
+ Reliability of dielectric thin films
- Physics and chemistry of gate dielectric breakdown, including
breakdown mechanisms, intrinsic vs. extrinsic failure, and failure analysis
- Reliability, yield and gate stack processing: growth conditions,
substrate effects, impurities, cleaning and plasma damage
- Relation of reliability to leakage and tunneling currents, charge
trapping and defect generation
- Special reliability considerations for ultrathin ( { 5nm) gate dielectrics
- Materials issues in hot-carrier reliability
+ Mechanical stress and strains in films, lines and device structures:
deformation, fracture, adhesion, simulation and modeling.
+ Novel analytical and measurement techniques
+ Reliability modeling and simulations


Joint sessions are planned with Symposium I: "Polycrystalline Thin Films III:
Structure, Texture, Properties, and Applications", and Symposium P:
"Science and Technology of Semiconductor Surface Preparation"

A partial list of invited speakers:
David Clarke (UCSB)
W.W. Gerberich (University of Minnesota)
Qing Ma (Intel)
J. Meindl (Georgia Tech University)
Wayne Paulson (Motorola)
Klaus Schuegraf (Micron)
M. Thouless (University of Michigan)
A.H. Verbruggen (Delft University)
W.L. Brown (Bell Laboratories)
J.E. Greene (University of Illinois)
C.V. Thompson (MIT)


Abstract information can be accessed through the MRS Website:

http://www.mrs.org/meetings/spring97/cfp/

Click on "abstract submission guidelines".

For further information, please contact Robert Keller (address, phone,
email given below).


==============================================================================
Robert R. Keller
National Institute of Standards and Technology
Materials Reliability Division, 853 office: (303)497-7651
325 Broadway FAX: (303)497-5030
Boulder, CO 80303 keller-at-boulder.nist.gov







From: bozzola-at-siu.edu (John J. Bozzola)
Date: Mon, 26 Aug 1996 17:42:07 -0600
Subject: Re: SEM conversion to digital image capture.

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Message-Id: {199608262109.OAA02848-at-scv1.apple.com}

Hi Alwyn,
We have been using the SEMICAPS system on hour Hitachi S570 for almost
three years now. No problems, works nicely, we're completely content with
it. The newer version of software is apparently even more user friendly.
John
(I have NO commercial interests in SEMICAPS--just a happy user.)



} Alwyn Eades eades-at-uimrl7.mrl.uiuc.edu
}
} Center for Microanalysis of Materials, University of Illinois,
} 104 S. Goodwin, Urbana, Illinois, 61801-2985, USA
} 217-333-8396, Fax 217-244-2278.
}
} We have a nine-year old SEM - a Hitachi S-800 - which we
} would like to convert to digital image capture. We would
} prefer to do this by buying a complete commercial package,
} rather than by putting together a system of our own.
}
} If you have had such a system installed on your SEM, please
} send me your experience and recommendations.

####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Mon, 26 Aug 1996 18:55:33 -0400 (EDT)
Subject: Re: Thumbs plus

Contents Retrieved from Microscopy Listserver Archives
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Confocal Microscopy List {confocal-at-ubvm.cc.buffalo.edu}


Oops. I can't type worth a .....

try http://206.66.184.152/index.html


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************

On Mon, 26 Aug 1996, Elinor Solit wrote:

} Jay,
}
} In reference to your message on the Microscopy Bulletin Board re the
} above, I tried the address in your message several times, and got not one
} flicker of life.
}
} Is this correct: http://206.66.154.152/index.html ?
}
} Many thanks. Our bulletin board address is cambrex-at-world.std.com
}
} Elinor Solit
} **********************************
} Elinor Solit
} (http://www.shorenet.~catalogs )
} **********************************
}
}
}
}




From: Mike Nicksic :      menco-at-azstarnet.com
Date: Mon, 26 Aug 1996 18:33:54 -0700
Subject: Web Sources

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Message-ID: {32225082.4E07-at-azstarnet.com}

What Web sites buy/sell/trade microscopes & accessories?

Mike Nicksic
menco-at-azstarnet.com




From: Woody.N.White-at-mcdermott.com
Date: 8/26/96 4:56 PM
Subject: Re[2]: Kevex/printer drv? -Reply

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In regard to locating a printer program/driver, RT-11 / custom
applications: Have not located such code and appears unlikely now
that I will. I did locate one outfit who would generate a program,
but for (about) $20,000!!!! A new EDS would be cheaper in the long
run....

I did purchase a Mitsubishi video printer. One must be careful which
video printer is selected, since the video sweep/sync from the Kevex
(and maybe Tracor?) are non standard rates. Although I like color, it
was not my primary concern for EM work. Given this, I chose the
Mitsubishi P-78U. This printer, is full-tone BW, fast, produces
relatively cheap hard copy (plastic "paper") and has a rather large
(for video printers) format... 6 x 8 inches. Cost here was about
$2750. Need to hook in series between computer and CRT.

Good luck all ... AND if anyone does find a driver program, I am
still interested..

Woody White
______________________________ Reply Separator _________________________________


X-Mailer: Novell GroupWise 4.1

** High Priority **

Woody,

I am also interested to configure a PDP-11 to a PC. We have a TN-5500
system. Also let me know which video and/or laser printer would you
select?

Thank you for the information,
Regards,

Laszlo J. Veto
Electron Microscopy and Image Analysis
AAFC




From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Tue, 27 Aug 1996 09:51:18 -0400 (EDT)
Subject: Immunocytochemistry

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We are starting to do immunocytochemistry in our EM lab using cryo
sections and plastic embedded sections, and I have a couple of questions
concerning both types....

1. Is it necessary to etch 1um sections embedded in Araldite for, say,
glutamate ? and if so, I have a protocol already, but could anyone
add their suggestions?

2. We are using Endothelin, and iNOS on frozen brain tissue fixed in 4%
Para, has anyone tried these and had success? I'm getting
staining with the iNOS, but the endothelin doesn't seem to work
very well, and I'm wondering what i am doing wrong.

3. Does anyone have a working protocol for immunogold using glutamate,
endothelin, or iNOS?

thank you.

Cheri Owen
Detroit Neurotrauma Institute
Detroit, Michigan
FAX (313)577-7552





From: JBG8NORD-at-aol.com
Date: Tue, 27 Aug 1996 10:40:53 -0400
Subject: Re: Web Sources

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On Tue, 27 Aug 96 Mike Nicksic wrote:

} What web sites buy/sell/trade microscopes & accessories.

Mike,

Try Microscopy Online (http://microscopy-online.com/).
This site is has a bulletin board for general postings including your area of
interest.

Jeff Goldstein
Nordcoff Associates




From: bozzola-at-siu.edu (John J. Bozzola)
Date: Tue, 27 Aug 1996 10:14:46 -0600
Subject: All:4x5 scanner

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Does anyone know if the Nikon LS-4500AF 4x5 negative scanner is, in fact,
the Leaf Scan (Scitex) unit in another skin? If so, can someone recommend a
vendor, please. Thank you.

####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: ruixia-at-ipas4.afip.mil (Ruixia Zhou)
Date: Tue, 27 Aug 1996 12:33:06 -0400
Subject: calibration slide

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Hi,

I am looking for a calibration slide for light transmittant microscope.
The slide should hold several absorbing objects with know optical
density and size. Ideally, multiple objects with various size,
optical density, and distances in a single transparant slide
is desirable.

Please forward any info. to ruixia-at-afip.mil. Your information is
appreciated. Thanks.

Dr. Ruixia Zhou
AFIP




From: Andre Wong :      andywong-at-unixg.ubc.ca
Date: Tue, 27 Aug 1996 11:54:16 -0700 (PDT)
Subject: Greeting

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Hi, Lazlo

How are you doing? It has been a long time that we have not talked to each
other. You must be enjoying life up there in Summerland. I heard lately that
Michael Weiss will be joing you soon.

I am still working at the EM facility in the department of Oral Biology.
Things has changed quite a lot here. I hope that I can handle all the
changes before my retirement.

Talk to you again.

Andre
Andre


Department of Oral Biology
Faculty of Dentistry
University of BC
2199, Wesbrook Mall, Vancouver, BC, Canada
phone # 822-2873
fax # 822-6698
e-mail andywong-at-unixg.ubc.ca





From: ROSEBUW :      rosebuw-at-aa.wl.com
Date: Tue, 27 Aug 1996 11:24:56 -0400 (EDT)
Subject: immunocyto for endothelin

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Mr-Received: by mta PDAV01.MUAS; Relayed; Tue, 27 Aug 1996 11:24:56 -0400
Mr-Received: by mta PDAV02; Relayed; Tue, 27 Aug 1996 11:24:56 -0400
Mr-Received: by mta SRVR01; Relayed; Tue, 27 Aug 1996 11:25:32 -0400
Disclose-Recipients: prohibited

Hi Cheri

I realize this isn't exactly the information you are looking for, but
it may be helpful.
On 10% formalin fixed, paraffin embedded rabbit iliac femoral arteries,
I used endothelin-1 monoclonal antibody from Biodesign, Cat# H55194M at
1:400 with fifteen minutes of trypsinization with good results.


Good luck
Wendy Rosebury
Warner-Lamber / Parke-Davis
rosebuw-at-aa.wl.com
(313) 996-7375


Cheri wrote:

We are starting to do immunocytochemistry in our EM lab using cryo
sections and plastic embedded sections, and I have a couple of questions
concerning both types....

1. Is it necessary to etch 1um sections embedded in Araldite for, say,
glutamate ? and if so, I have a protocol already, but could anyone
add their suggestions?

2. We are using Endothelin, and iNOS on frozen brain tissue fixed in 4%
Para, has anyone tried these and had success? I'm getting
staining with the iNOS, but the endothelin doesn't seem to work
very well, and I'm wondering what i am doing wrong.

3. Does anyone have a working protocol for immunogold using glutamate,
endothelin, or iNOS?

thank you.

Cheri Owen
Detroit Neurotrauma Institute
Detroit, Michigan
FAX (313)577-7552





From: P.Wyeth-at-soton.ac.uk (Paul Wyeth)
Date: Tue, 27 Aug 1996 23:46:52 +0100
Subject: research assistant

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*******************************************************
Research Assistant in Bioceramic Characterisation

Applied Biocomposites Group
Department of Chemistry
University of Southampton
Southampton SO17 1BJ
U.K.

Applications are invited for the post of Research Assistant (grade 1B,
salary =A314317) within the Applied Biocomposites Group, to work as part of=
a
small team on the characterisation of robust bioceramics. Funds from the
EPSRC Nanotechnology programme are available for two years commencing 1st
January, 1997.

A range of techniques, including scanning and transmission electron
microscopy, atomic force microscopy and electron microprobe analysis, will
be applied to the elucidation of the microstructural and ultrastructural
architecture of the biocomposites as well as their crystallochemistry.
Prospective candidates should, preferably, have experience of one or more of
these techniques and the accompanying methodology of specimen preparation.

Part-time registration for a higher degree is possible. Further details can
be seen at http://www.soton.ac.uk/~pw/abg/posts. Applicants should send
their CVs and the names of two referees to Dr Paul Wyeth by 15th October,
1996; preliminary, informal enquiries are welcome (email: pw-at-soton.ac.uk).

The University of Southampton is an equal opportunities employer.

*****************************************************
Dr Paul Wyeth

Applied Biocomposites Group
Department of Chemistry,
University of Southampton,
Southampton SO17 1BJ,
U.K.

Tel: +44 (0)1703 592186
Fax: +44 (0)1703 593781
URL: http://www.soton.ac.uk/~guid/
email: pw-at-mail.soton.ac.uk





From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Tue, 27 Aug 1996 12:55:55 -0600
Subject: Re: Web Sources

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Message-Id: {199608272349.SAA12441-at-Sparc5.Microscopy.Com}
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Try the following:
http://www.capovani.com
http://www.zeiss.com

===================================

} What Web sites buy/sell/trade microscopes & accessories?
}
} Mike Nicksic
} menco-at-azstarnet.com

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(630)252-7194
FAX: (630)252-4798






From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Tue, 27 Aug 1996 12:57:40 -0600
Subject: Re: All:4x5 scanner

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Message-Id: {199608272351.SAA12450-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

It is NOT the LeafScan 45. The two have very different capabilities.
========================
} Does anyone know if the Nikon LS-4500AF 4x5 negative scanner is, in fact,
} the Leaf Scan (Scitex) unit in another skin? If so, can someone recommend a
} vendor, please. Thank you.
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Center for Electron Microscopy
} Southern Illinois University
} Carbondale, IL 62901-4402
} U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(630)252-7194
FAX: (630)252-4798






From: W.Jablonski-at-csl.utas.edu.au (Wis Jablonski)
Date: Wed, 28 Aug 1996 15:58:18 +1100
Subject: E-mail

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Dear All,
Please help me to find E-mail address of Prof. M. Isaacson of Cornell
University, School of Applied and Engineering Physics , Ithaca, NY ( address
and institution valid as per July 1994)
I can be contacted directly or via our network.
Thank you
Wis Jablonski, OiC EM/X-ray Microanalysis at CSL University of Tamania,
Australia





From: liuying-at-mb.luth.se (Liu-Ying Wei)
Date: Wed, 28 Aug 1996 08:59:07 +0800
Subject: Re: Web Sources

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Try Microscopy Online (http://microscopy-online.com/).
This site is has a bulletin board for general postings including your area of
interest.

Jeff Goldstein
Nordcoff Associates

Try the following:
http://www.capovani.com
http://www.zeiss.com

Mike,

===================================

} What Web sites buy/sell/trade microscopes & accessories?
}
} Mike Nicksic
} menco-at-azstarnet.com

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(630)252-7194
FAX: (630)252-4798






From: Andreas Brech :      andreas.brech-at-bio.uio.no
Date: Wed, 28 Aug 1996 13:02:19 +0100
Subject: Re: Web Sources

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subscribe microscopy andreas.brech-at-darwin.uio.no





From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Wed, 28 Aug 1996 11:24:34 -0400
Subject: Re[2]: EM courses

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} Return-Path: {TLREYN-at-ccmail.monsanto.com}
} From: TLREYN-at-ccmail.monsanto.com
} X400-Originator: TLREYN-at-MONSANTO.COM

}
} Do you have any info regarding other EM courses that are given around the
} country or where I can find out about it???
} Tracey Reynolds
}
}
}
Please reply to Ms Reynolds directly
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: hong-at-apollo.numis.nwu.edu (Hong Zhang)
Date: Tue, 27 Aug 1996 17:25:17 -0500
Subject: unsubscribe

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unsubscribe hong-at-apollo.numis.nwu.edu




From: Xianying Burany :      xianying-at-udel.edu
Date: Wed, 28 Aug 1996 13:12:25 -0400 (EDT)
Subject: Dimpling

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Dear All:

We want buy a used or a new dimpling instrument to make cross sectioning
TEM samples and a 1700 C heat treatment furnace. Please reply directly to
me.

I appreciate your help

Sandy Burany, PhD
Dept. of Physics & Astronomy
Univ. of Delaware
Newark, DE 19711
Email: 94765-at-udel.edu or xianying-at-udel.edu
(302) 831-3515
Fax: (302) 831-1637





From: Paulo Iris :      piris-at-baitaca.ipen.br
Date: Wed, 28 Aug 1996 15:25:16 -0300 (ADT)
Subject: Dimpling

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UNSUBSCRIBE





From: Paulo Iris :      piris-at-baitaca.ipen.br
Date: Wed, 28 Aug 1996 15:28:25 -0300 (ADT)
Subject: UNSUBSCRIBE

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From: Krueger, Eugene :      krueger.eugene-at-mayo.edu
Date: 28 Aug 1996 13:17:35 -0600
Subject: stereololgy tool

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Message-ID: {n1370884250.2044-at-msgw.mayo.edu}

I need a tool (non-computer) that will allow me to trace a shape and get some
kind of distance travelled measurement. I remember a special pen that had a
rollerball in the tip with a digital readout on the shaft, but I cannot locate
one or even a catalog with anything similar. Please help!!! Any product with
a similar ability would also work.

TIA

Eugene Krueger
GI Research
Mayo Foundation
krueger.eugene-at-mayo.edu




From: Peter Markiewicz :      pmarkiew-at-chem.utoronto.ca
Date: Wed, 28 Aug 1996 15:37:43 -0400
Subject: Using TEM grids with the AFM

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Further to the message sent by Don Chernoff to Jouko K. M=E4ki on using TEM
grids with the atomic force microscope:

Our group has recently submitted a paper on this very subject. Some of the
highlights of this can be found at my Web site:

http://www.chem.utoronto.ca/~pmarkiew/publctns/grids.html

It contains some large pictures, so the downloading may take some time, but
the setup and some of the results we obtained are given.

BTW - Could someone send me the address or phone number for Alltech, the
supplier of 400 mesh indexed grids (I've been using 200 mesh).

Peter.


Peter Markiewicz Voice (416) 978-4526
Department of Chemistry Fax (416) 978-6254
University of Toronto pmarkiew-at-chem.utoronto.ca
80 St. George Street http://alchemy.chem.utoronto.ca/~pmarkiew
Toronto, Canada M5S 1A1





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 28 Aug 1996 16:44:34 -0400
Subject: None

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From: Paulo Iris :      piris-at-baitaca.ipen.br
Date: Wed, 28 Aug 1996 15:25:16 -0300 (ADT)
Subject: None

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UNSUBSCRIBE MICROSCOPY Paulo Iris piris-at-baitaca.ipen.br


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From: P.Wyeth-at-soton.ac.uk (Paul Wyeth)
Date: Wed, 28 Aug 1996 23:28:19 +0100
Subject: research assistant

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


*******************************************************
Research Assistant in Bioceramic Characterisation

Applications are invited for the post of Research Assistant (grade 1B,
salary 14317 pounds) within the Applied Biocomposites Group, to work as part
of a small team on the characterisation of robust bioceramics. Funds from
the EPSRC Nanotechnology programme are available for two years commencing
1st January, 1997.

A range of techniques, including scanning and transmission electron
microscopy, atomic force microscopy and electron microprobe analysis, will
be applied to the elucidation of the microstructural and ultrastructural
architecture of the biocomposites as well as their crystallochemistry.
Prospective candidates should, preferably, have experience of one or more of
these techniques and the accompanying methodology of specimen preparation.

Part-time registration for a higher degree is possible. Further details can
be seen at http://www.soton.ac.uk/~pw/abg/posts. Applicants should send
their CVs and the names of two referees to Dr Paul Wyeth by 15th October,
1996; preliminary, informal enquiries are welcome (email: pw-at-soton.ac.uk).

The University of Southampton is an equal opportunities employer.

*****************************************************
Dr Paul Wyeth

Applied Biocomposites Group
Department of Chemistry,
University of Southampton,
Southampton SO17 1BJ,
U.K.

Tel: +44 (0)1703 592186
Fax: +44 (0)1703 593781
URL: http://www.soton.ac.uk/~guid/
email: pw-at-mail.soton.ac.uk





From: Jay Jerome :      jjerome-at-bgsm.edu
Date: Wed, 28 Aug 1996 21:45:44 -0400 (EDT)
Subject: Re: stereololgy tool

Contents Retrieved from Microscopy Listserver Archives
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We purchased such a tool at our local bookstore in the road map section.
It is intended as a map wheel for measuring distances on maps. I have
also seen them in travel supply and luggage shops. We use ours in
several measuring situations. However, for measuring
circumferences of irregular objects the stereological point counting
procedure still works better. Although it gives aggregate data (total
circumference for a group of objects and only a relative measure
(i.e. distance per area) you can mathematically calculate the distance if
you know the area.


Jay Jerome
**************************************************************
* aka: W. Gray Jerome *
* Dept. of Pathology *
* Bowman Gray School of Medicine of Wake Forest University *
* Medical Center Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972 *
* jjerome-at-bgsm.edu *
**************************************************************

On 28 Aug 1996, Krueger, Eugene wrote:

} I need a tool (non-computer) that will allow me to trace a shape and get some
} kind of distance travelled measurement. I remember a special pen that had a
} rollerball in the tip with a digital readout on the shaft, but I cannot locate
} one or even a catalog with anything similar. Please help!!! Any product with
} a similar ability would also work.
}
} TIA
}
} Eugene Krueger
} GI Research
} Mayo Foundation
} krueger.eugene-at-mayo.edu
}




From: W.Jablonski-at-csl.utas.edu.au (Wis Jablonski)
Date: Thu, 29 Aug 1996 09:54:50 +1100
Subject: E-mail address

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Message-Id: {199608282250.IAA08968-at-lab.csl.utas.edu.au}
X-Sender: wis-at-postoffice.csl.utas.edu.au
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Thank you, All, for your fantastic response to my enquires about Prof. Isaacson
E-mail address. I got now four different versions of it and will try the lot..
Thanks again,
Wis Jablonski, University of Tasmania





From: STANSMAN-at-aol.com
Date: Thu, 29 Aug 1996 00:19:39 -0400
Subject: New Nikon Inc. Web Site

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To all Microscopists,

For informational and reference purposes Nikon Inc. would like to invite you
to visit our new WEB site at http://www.nikonusa.com

Join us in our world wide introduction of the New Eclipse E800 microscope and
CFI60 optical system.

Comments and requests can be made to me directly at NikonBio-at-aol.com

Thank You,

Stan Schwartz
Manager, Biomedical Instruments
Nikon Inc. Instrument Group
1300 Walt Whitman Rd.
Melville, NY 11747
516-547-8529 Fax 516-547-0306
E-mail NIKONBIO-at-aol.com





From: Woody.N.White-at-mcdermott.com
Date: 8/28/96 10:50 AM
Subject: Kevex RT-11 files to PC for printout

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Hello Jeff...

Been there....have that.... In fact have 2 PCs which can read the
44s, but horribly inconvenient. Not allowed to hook-up to "rest of
world" so networking is no-go. Also have aspect ratio distortion when
manipulating 8000 data on PC with 3rd party software.

Thanks, however, for the reply..... Woody White

Babcock & Wilcox Co
Lynchburg Research Center
Lynchburg, VA 24506

______________________________ Reply Separator _________________________________


X-Mailer: NetConnect (c)Thinque 1995
Mime-Version: 1.0
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I noticed your posting on the Microscopy forum and thought you may be
interested in some software and hardware options available from Kevex.
There is a software product called Report Manager that runs on the
Delta or 8000 analyzer under RT-11 or TSX+ and allows the operator to
convert Kevex image and spectra files to TIFF format. There are also
hardware products which allow transfer of data files (TIFF, text, or
raw binary) from the DEC RT-11 system to a PC. One such product is the
PC Communications Package, using Kermit, but it is fairly slow at 19.2
kbps. The other product is SCSI based and can transfer an entire image
file in only a few seconds. Customers who have upgraded to the Kevex
Sigma analyzer find this especially useful for sharing files between
the older analyzer and the Sigma. If you are interested, your
regional Kevex Service office would be happy to provide pricing and
more details.

Best regards,

Jeff Allbright




From: Simon.Dumbill-at-aeat.co.uk (Simon Dumbill)
Date: 27/08/96 12:57
Subject: Re: All:4x5 scanner

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Mime-Version: 1.0

I'd be interested to hear just what the 'different capabilities' are
and any opinions on the relative merits of negative scanners.


Simon Dumbill
AEA Technology plc Tel: +44 1235 434245
220, Harwell Fax: +44 1235 435941
Didcot Email: Simon.Dumbill-at-aeat.co.uk
Oxfordshire OX11 0RA
UK




______________________________ Forward Header __________________________________


It is NOT the LeafScan 45. The two have very different capabilities.
========================
} Does anyone know if the Nikon LS-4500AF 4x5 negative scanner is, in fact,
} the Leaf Scan (Scitex) unit in another skin? If so, can someone recommend a
} vendor, please. Thank you.
}
} ####################################################################
} John J. Bozzola, Ph.D., Director
} Center for Electron Microscopy
} Southern Illinois University
} Carbondale, IL 62901-4402
} U.S.A.
} Phone: 618-453-3730
} Fax: 618-453-2665
} Email: bozzola-at-siu.edu
} Web: http://www.siu.edu/departments/shops/cem.html
} ####################################################################

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(630)252-7194
FAX: (630)252-4798






From: John Best :      jbest-at-vicon.net
Date: Thu, 29 Aug 1996 08:26:09 -0700
Subject: Image networking from PC to MAC

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Message-ID: {3225B691.1F79-at-vicon.net}

Good Day Everyone,

We are considering establishing some sort of dial in connection between
my SEM lab and a 4th grade classroom. The teacher wishes to send samples
home with my daughter semi-interactively send images back to the
classroom. They have a phone in the classroom and several MACs which
are linked together with a very slow AppleTalk network.

I think we'd like to put a 28.8 modem on one of the MAC's to dial in to.
The software issue is the main concern. Could anyone please make
reccomendations as to some sort of simple networking software that would
allow us to at least transfer the image files?

Thanks for your thoughts on this.
Regards,
John

--
John W. Best ELMDAS Co. Email: jbest-at-vicon.net
P.O. Box 355, Alexandria, PA, USA 16611
Voice: 814-669-4474
WWW: http://www.vicon.net/~jbest





From: Xianying Burany :      xianying-at-udel.edu
Date: Thu, 29 Aug 1996 08:27:16 -0400 (EDT)
Subject: Dimpling

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Dear All:

We want to buy a used or a new dimpling instrument to make cross
sectioning TEM samples, and a 1700 C heat treatment furnace. If you have
any information please reply directly to me.

I appreciate your help

Sandy Burany, PhD
Dept. of Physics & Astronomy
Univ. of Delaware
Newark, DE 19711
Email: 94765-at-udel.edu or xianying-at-udel.edu
(302) 831-3515
Fax: (302) 831-1637





From: geosclmr-at-showme.missouri.edu (Lou Ross)
Date: Thu, 29 Aug 1996 08:43:28 -0500
Subject: Kevex 4505P

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I'm looking for a Kevex 4505P pulse processor to resurrect a 7000 EDS
system. If anyone out there has a spare or leads for me to contact, please
respond to me directly.

TIA,
Lou Ross

Electron Beam Analytical Facility
101 Geological Sciences Bldg.
University of Missouri
Columbia, MO 65211
(573) 882-4777, 882=5458 fax

http://www.missouri.edu/~geosclmr/ebaf.html

'Hindsight... foresight...
sometimes we have no sight at all.'






From: ebs-at-ebsciences.com
Date: Thu, 29 Aug 1996 08:39:21 -0500
Subject: Re: stereololgy tool

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Message-Id: {199608291450.AA17787-at-gateway.ppg.com}

At 01:17 PM 8/28/96 -0600, you wrote:
} I need a tool (non-computer) that will allow me to trace a shape and get some
} kind of distance travelled measurement. I remember a special pen that had a
} rollerball in the tip with a digital readout on the shaft, but I cannot locate
} one or even a catalog with anything similar. Please help!!! Any product with
} a similar ability would also work.
}
Hi Eugene-

The device you are looking for is called a "curvimeter" or "plan measure."
We have several styles available in our catalog (on-line at
http://www.ebsciences.com).

Best regards
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: kna101-at-utdallas.edu
Date: Thu, 29 Aug 1996 09:07:39 -0500 (CDT)
Subject: Re: stereololgy tool

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Eugene,

What you're talking about is a map wheel. Aplace like the Sharper Image
may have one, otherwise try a store that sells travel equipment.
Unfortunately, I haven't seen one in the scientific catalogs. Good luck.

Karen Pawlowski

On 28 Aug 1996, Krueger, Eugene wrote:

} I need a tool (non-computer) that will allow me to trace a shape and get some
} kind of distance travelled measurement. I remember a special pen that had a
} rollerball in the tip with a digital readout on the shaft, but I cannot locate
} one or even a catalog with anything similar. Please help!!! Any product with
} a similar ability would also work.
}
} TIA
}
} Eugene Krueger
} GI Research
} Mayo Foundation
} krueger.eugene-at-mayo.edu
}




From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Thu, 29 Aug 1996 08:56:16 -0500
Subject: Re: stereololgy tool

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Message-Id: {199608291350.IAA07392-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} I need a tool (non-computer) that will allow me to trace a shape and get some
} kind of distance travelled measurement. I remember a special pen that had a
} rollerball in the tip with a digital readout on the shaft, but I cannot locate
} one or even a catalog with anything similar. Please help!!! Any product with
} a similar ability would also work.
}
} TIA
}
} Eugene Krueger

Try the yuppie catalogs like Herrington's, Improvements, etc., in
th automotive section--items like this a sold for measuring mileages on
roadmaps. You might also try the catalog ads in the back of "Earth" and
"Natural History".
I've also found such devices in the drafting section of art supply
stores, architecture/drafting supply stores, etc., where the same items are
sold, except now the official purpose is measuring perimeters, etc. on
plans.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu
(217) 355-1143 home

*********** looking for a job again ***********






From: John F. Mansfield :      jfmjfm-at-umich.edu
Date: Thu, 29 Aug 1996 10:18:06 -0400
Subject: Microsscopy & Microanalysis on the World Wide Web

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X-Sender: jfmjfm-at-srvr5.engin.umich.edu
Message-Id: {v03007809ae4b56457d42-at-[141.213.21.13]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hi there,
I have had a number of requests to make available the presentations and
documents used in the First Microbeam Analysis Society Topical Conference
Microsscopy & Microanalysis on the World Wide Web. I have therefore
created a number of Web Pages that feature most of the presentations that
were given. They are in html, Adobe PDF and Microsoft Powerpoint (the
latter two have free viewers available).
To view these "Proceedings" files please connect to:

http://www.microanalysis.org/mas/mandmonthewww/

Comments, moans and groans and compliments to me jfmjfm-at-umich.edu.

Cheers
Jfm.



John Mansfield
North Campus Electron Microbeam Analysis Laboratory
417 SRB, University of Michigan
2455 Hayward, Ann Arbor MI 48109-2143
Phone: (313)936-3352 FAX (313)936-3352
Email: jfmjfm-at-engin.umich.edu
URL: http://emalwww.engin.umich.edu/people/jfmjfm/jfmjfm.html






From: John Best :      jbest-at-vicon.net
Date: Thu, 29 Aug 1996 12:29:30 -0700
Subject: Re: Image networking from PC to MAC

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Message-ID: {3225EF9A.4241-at-vicon.net}

Dear Phil and All,

Phil wrote:
Please post responses to this question to the list, or copy your replies
to me, I am interested in this problem myself. Phil

I did receive (not via the microscopy listserver) this response which I'm
assuming Peter Guthrie wouldn't mind my forwarding. Thanks Peter.

} From Peter:
Try Timbuktu (from Farallon Software). We actually use it to control
a remote Macintosh-based image acquisition system. Timbuktu puts a
mirror of the remote Macintosh desktop on your computer, so images
brought up on the remote computer are seen on the local computer.
Peter Guthrie

Also, Lou Ross suggested a shareware program called "Fetch". Could
anyone comment on this? Is it something like "PC anywhere"? Thanks Lou.

To everyone else who could help with this problem, please post your
replies to the listserver, as the problem seems to have applicability to
a number of us.

Have a good day everyone. Regards, John.
--
John W. Best ELMDAS Co. Email: jbest-at-vicon.net
P.O. Box 355, Alexandria, PA, USA 16611
Voice: 814-669-4474
WWW: http://www.vicon.net/~jbest





From: Warren Straszheim :      wes-at-ameslab.gov
Date: Thu, 29 Aug 1996 08:41:09 -0500
Subject: Re: Kevex RT-11 files to PC for printout

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At 04:14 PM 8/28/96 -0500, you wrote:
} Hello Jeff...
}
} Been there....have that.... In fact have 2 PCs which can read the
} 44s, but horribly inconvenient. Not allowed to hook-up to "rest of
} world" so networking is no-go. Also have aspect ratio distortion when
} manipulating 8000 data on PC with 3rd party software.

Hello again, Woody.
Kevex stretches their pixels vertically. They fit 512 pixels into a 4-unit
wide screen and 256 into the 3 units of height. You would need 384 pixels
vertically to get square pixels.

I have recently processed a few maps that we converted to TIF and shipped to
a PC. My processing involves cropping off the unused right section of the
image (it is there for my conversion software if not Kevex's), and then
resizing the image to 384 pixels high to give the right overall aspect ratio
with square pixels. It is a kluge fix, but it works for reports, and these
images are small compared to what we collect on our newer EDS system.
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Terry.R.McCue-at-mcdermott.com
Date: 29 Aug 96 11:03:00 -0500
Subject: Microanalysis Services

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Colleagues

Our lab is looking for microanalysis services :

* Wish to analyze grain boundaries of Alloy 690 materials; objective
is to compare composition of grain boundary regions which exhibit
differences in metallographic etching response ( 2% Bromine-Methanol
Etchant).

* Interested in applying:

Main Interest - * SIMS imaging for element distribution

* APFIM atom probe field ion microscopy

* Analytical transmission electron microscopy of thinned foils.

** (Reference: MET & MATERIALS TRANSACTIONS A, Volume 27A, Feb. 1996,
page 327

We'd like to identify potential services vendors.

Need to define scope of the deliverable from each test and per
specimen cost.


Larry W. Sarver & Terry R. McCue
Metallurgical Analysis Section
Babcock & Wilcox Research
1562 Beeson St.
Alliance, Ohio 44601
(330) 829-7463 or 7427 Fax: (330) 829-7831
Internet: larry.w.sarver-at-mcdermott.com
terry.r.mccue-at-mcdermott.com




From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: 8/28/96 1:17 PM
Subject: stereololgy tool

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Mime-Version: 1.0
"Krueger; Eugene" {krueger.eugene-at-mayo.edu}
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
Content-Description: cc:Mail note part

Eugene;

Sounds like you are interested in some sort of planimeter. I have the
address of a company that offered such a product in the past, but I have
not had contact with them in recent years. The product was a Model 10
Lasico polar planimeter. Their last known address was:

Los Angeles Scientific Instrument Co.
2451 Riverside Dr.
Los Angeles, CA 90039
Ph: 662-2128 (area code may be 213 or 818, not sure)

Good luck;

-Bob
********************************
Bob Citron
Chiron Vision Corp.
555 W. Arrow Hwy
Claremont, CA 91711
USA
Ph: (909)399-1311
email: Bob_Citron-at-cc.chiron.com
********************************

______________________________ Reply Separator _________________________________


I need a tool (non-computer) that will allow me to trace a shape and get some
kind of distance travelled measurement. I remember a special pen that had a
rollerball in the tip with a digital readout on the shaft, but I cannot locate
one or even a catalog with anything similar. Please help!!! Any product with
a similar ability would also work.

TIA

Eugene Krueger
GI Research
Mayo Foundation
krueger.eugene-at-mayo.edu




From: Warren Straszheim :      wes-at-ameslab.gov
Date: Thu, 29 Aug 1996 09:13:19 -0500
Subject: Re: Image networking from PC to MAC

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Well after getting a dose of Telepresence Microscopy at the MSA meeting, I
have been bitten by the bug. There was much reference to Timbuktu Pro there
for remote control and/or observation. It piqued my interest enough to check
out such programs and to try the demos when available. (Timbuktu has a
30-day free trial version available through their web site
http://www.farallon.com)

Trying it out, I see it allows for file transfers back and forth between
MACs and PCs as well as the control and observe options. It also has a
message capability which can drag along files with it. I have been duly
impressed, and the $49 on-line price for the PC version doesn't hurt too
much. It was $70 per copy through PC Connection. I have been using it over
the ethernet (in IP mode), but it also works over dial-up with the right
supporting software.

In fairness, there are at least two similar products: PC Anywhere from
Symantec and Carbon Copy from MicroCom. The capabilities all look good.
however, the demos were not quite as functional, and I don't think they
supported MAC to PC communications. Prices were comparable.

BTW, can anyone tell me if there are certain window or screen displays that
are not supported by such products? Some of our live acquition Windows don't
come across well or at all.

Disclaimer - I have no financial interest in any of the above-mentioned
companies. Kind of wish that I did. They look like hot products.

At 08:26 AM 8/29/96 -0700, John Best wrote:
} Good Day Everyone,
}
} We are considering establishing some sort of dial in connection between
} my SEM lab and a 4th grade classroom. The teacher wishes to send samples
} home with my daughter semi-interactively send images back to the
} classroom. They have a phone in the classroom and several MACs which
} are linked together with a very slow AppleTalk network.
}
} I think we'd like to put a 28.8 modem on one of the MAC's to dial in to.
} The software issue is the main concern. Could anyone please make
} reccomendations as to some sort of simple networking software that would
} allow us to at least transfer the image files?
}
} Thanks for your thoughts on this.
} Regards,
} John
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: drstad-at-JUNO.COM (David R Stadden)
Date: Thu, 29 Aug 1996 17:14:47 EDT
Subject: Imaging PE Foam Cells

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To: Microscopy-at-Sparc5.Microscopy.Com

I have a need to get an image of polyethylene foam cells in order to
allow subsequent image processing for cell size, area, etc. I don't want
anything but the 2-D outline of the cells -- no glare from the backs of
the closed cells, for example. The magnification needed would probably
be around 20X-100X, and the polymer is gray in color. I've tried using
loose carbon black to render the surfaces dull black, but not enough
adheres. I tried using soot from a flame, but again no luck. I suppose
some type of staining or embedding would be the way to go, and wonder if
the histologists in the group might have a technique that could be
useful. SEM is available to generate the image, too, but I was hoping
for a light microscopy method. Any ideas would be much appreciated.

Dave Stadden
DRStad-at-Juno.com




From: Robert Derby :      derby-at-pb.net
Date: Thu, 29 Aug 1996 17:49:37 -0500
Subject: CCD cam.

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Anyone have any experience with a "Pixera" CCD, Pro's and Con's welcomed.
Thanks
Robert derby





From: mmdisko-at-erenj.com (Mark M Disko)
Date: Thu, 29 Aug 1996 11:31:09 -0400
Subject: TEM Position at Exxon Research

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Message-Id: {199608291529.LAA15082-at-eredns.erenj.com}
X-Sender: mmdisko-at-clmail.erenj.com
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Please Note: I am posting this position announcement on August 29 to
get quick responses as the position is open immediately. I will be away from
the office and not checking electronic mail until Monday September 9.
Please send resumes to the address given below.

- Mark Disko


-----------------------------------------------------------------------------

POSITION IN ANALYTICAL TRANSMISSION ELECTRON MICROSCOPY

Corporate Research Laboratory
Exxon Research and Engineering Company

The position will involve the use of high resolution and
analytical transmission electron microscopy for characterizing
catalysts, polymers, engineering materials, and other materials of
interest to Exxon. Requirements include expertise with high
resolution electron microscopy, electron diffraction for phase
identification, digital image processing, energy-dispersive x-ray
analysis, scanning TEM, and electron energy-loss spectrometry.
Experience with the materials science of heterogeneous catalysts
is desirable for this rapidly advancing research program.
Research in this position involves a multidisciplinary team
approach which provides an excellent learning environment. Our
laboratory offers advanced instrumentation for structure-property
studies of complex materials, including a new field-emission TEM
equipped with a rotatable electron biprism for electron
holography.

A Masters degree or equivalent working experience beyond the
Baccalaureate degree in materials science, physics, chemistry or
related fields form requirements for this position.

Our research lab in rural New Jersey offers convenient access to
Philadelphia, Princeton and New York City. Exxon offers an
excellent working environment, salary commensurate with skills and
experience, and excellent benefits.

Applicants for this position who meet the majority of the
qualifications outlined above should forward a resume, publication
list and two or three letters of reference to:

Dr. Mark M. Disko
Corporate Research Laboratory
Exxon Research and Engineering Company
1545 Route 22 East
Annandale, New Jersey 08801

(908) 730-2503
FAX (908) 730-3314

----------------------------------------------------------------------

Please do not respond directly to this list server. In the
event you need further information rapidly, forward electronic
mail to me at mmdisko-at-erenj.com . I will however not be able to
respond until sometime after September 9, 1996.



Equal Opportunity Employer M/F/H/V







From: Greg2NJ-at-aol.com
Date: Thu, 29 Aug 1996 19:27:44 -0400
Subject: Microwave #1

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Question Set ONE:
Our Lab is currently investigating the possibilities of incorporating
microwave technology in Electron Microscopy. Would be interested in hearing
from other EM labs using microwave processing for biological applications
regarding the pro’s and cons.


Some questions I have at the moment are:
How do you en bloc stain with uranyl acetate, what percent, how much time to
microwave exposure, and the ideal staining temperature in the MW oven

Polymerizing EM blocks in the MW. Has anyone found a preference for using a
water load? Any success with Embed 812 kit, were there any NMA problems.

Any preference with EMBED 812/Araldite 502, if so the time and power, i.e. 15
min 50% power using a 700 watt MW oven.





From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Fri, 30 Aug 1996 10:08:52 +1000 (EST)
Subject: Re: Imaging PE Foam Cells

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John Russ used a stamp pad to look at foam structure in bread. He pressed
the bread on to a pad which made the cut surface nice and black. Worth a try.

mel dickson





From: Greg2NJ-at-aol.com
Date: Thu, 29 Aug 1996 19:27:49 -0400
Subject: Microwave #2

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Question Set TWO:
Our Lab is currently investigating the possibilities of incorporating
microwave technology in Electron Microscopy. Would be interested in hearing
from other EM labs using microwave processing for biological applications
regarding the pro’s and cons.

What is the better mode for fixation, time or temperature ?, i.e. time of
fixation in the MW vs. change in temperature of fixative in MW, or a
combination.

Does anyone have any methods for "processing" in the MW
i.e. times, temp, etc. for buffer washes, dehydration (acetone or alcohol),
infiltration schedules for biological applications, (liver, kidney, spleen).

Is there any way to increase the oven capacity (total number of blocks/day or
blocks/run?

Some references state the importance of CACl2 or MgCl2 for fixation and post
fixation, any thoughts?







From: ainswort-at-geology.gla.ac.uk (Pete Ainsworth)
Date: Fri, 30 Aug 1996 09:47:59 +0100
Subject: Re: Image networking from PC to MAC

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Hi John

There's a wide range of possible methods to do this. A lot depends on what
software you and the school have allready. I've listed some possibilities

Has the school access to e-mail? If so why not simply mail the images as
attachments. Choose an appropriate file format for the software the school
has. (if they do not have appropriate software for imaging - download NIH
IMAGE - http://rsb.info.nih.gov/nih-image/ - it's public domain and there
are a range of versions for the mac - if the school has older macs use one
of the older versions).

Likewise if the school has access to the WEB why not create some pages at
your end with the images on them. Convert the images to GIF format and
reduce their size as much as possible to retain reasonable images. (Adobe
photoshop is a great program for playing around with digital images
versions are available for Mac, Windows and UNIX - it is reasonably priced
and I for one have found it extremely useful).

I hope this is of some help - without more information it is hard to be
more specific - why not try speaking to the computing support at your end.

Good luck

Pete Ainsworth


} Good Day Everyone,
}
} We are considering establishing some sort of dial in connection between
} my SEM lab and a 4th grade classroom. The teacher wishes to send samples
} home with my daughter semi-interactively send images back to the
} classroom. They have a phone in the classroom and several MACs which
} are linked together with a very slow AppleTalk network.
}
} I think we'd like to put a 28.8 modem on one of the MAC's to dial in to.
} The software issue is the main concern. Could anyone please make
} reccomendations as to some sort of simple networking software that would
} allow us to at least transfer the image files?
}
} Thanks for your thoughts on this.
} Regards,
} John
}
} --
} John W. Best ELMDAS Co. Email: jbest-at-vicon.net
} P.O. Box 355, Alexandria, PA, USA 16611
} Voice: 814-669-4474
} WWW: http://www.vicon.net/~jbest

**************************************
* Pete Ainsworth *
* Dept. Geology & Applied Geology *
* Lillybank Gardens *
* University of Glasgow *
* Glasgow G12 8QQ *
* Tel : 0141 330 5505 (direct) *
**************************************






From: Crossman, Harold :      crossman-at-rd.sylvania.com
Date: Fri, 30 Aug 1996 07:07:00 -0400
Subject: imaging to/from school

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Message-Id: {c=US%a=_%p=SYLVANIA%l=SYLVANIA/OSI/00004271-at-da-exc1.sylvania.com}

John,

Part or all of your solution may be found in a shareware product called
CU-SeeMe, from Cornell University. There is also a commercial version
available from White Pine Software in Nashua, NH. White Pine has a lot
of experience in multiple platform communication.

I have no financial interest in White Pine, blah, blah blah...

The WWW page below is a wealth of information about the product. There
are many links to K-12 schools that have done just what you are trying
to do. I haven't used the software, but it looks pretty cool.

http://cu-seeme.cornell.edu/~WCW/

-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.osram.de
www.siemens.com





From: dlietz-at-trentu.ca (deborah Lietz)
Date: Fri, 30 Aug 1996 08:54:00 +0100
Subject: digital equipment

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We are still taking our time deciding on how we are going to leap into the
digital era so I'm looking for pros and cons on SEMICAPS , ORION AND THE
LEAF MICROLUMINA.



THANKS

Debbie Lietz
Electron Microscopy Suite
Department of Biology
Trent University
Peterborough, Ontario
K9J 7B8
Telephone: (705)748-1486
Fax: (705) 748-1205
email: dlietz-at-trentu.ca






From: Woody.N.White-at-mcdermott.com
Date: 8/29/96 4:14 PM
Subject: Imaging PE Foam Cells

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Dave,

Although I have not specifically tried this w/poly foam, I have
prepared other samples for such an examination. This will work best
if you have connected porosity. ....Impregnated the sample with a
material (low viscosity) which had a different average atomic number,
then cut or gring/polish a surface. Used low kV SEM-BSE to image the
structure. This may work for your poly, but will be a real
"challenge" compared to the specimens I had.

Woody White


______________________________ Reply Separator _________________________________


X-Mailer: Juno 1.15
X-Juno-Line-Breaks: 10-13

I have a need to get an image of polyethylene foam cells in order to
allow subsequent image processing for cell size, area, etc. I don't
want ...snip...
Dave Stadden
DRStad-at-Juno.com




From: kna101-at-utdallas.edu
Date: Fri, 30 Aug 1996 08:53:41 -0500 (CDT)
Subject: ROTO

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Hello,

Has anyone heard of a contrast enhancing stain for TEM on
biological sections called ROTO? If so, I'd like to know more about it.
A reply to the list would be very helpfull.

Karen




From: minter-at-kodak.com (John Minter)
Date: Fri, 30 Aug 1996 10:44:29 -0400
Subject: Re: Imaging PE Foam Cells

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Dave Stadden wrote:
I have a need to get an image of polyethylene foam cells in order to
allow subsequent image processing for cell size, area, etc. ...

I recall seeing a poster at an EMSA meeting several years ago that provided
an elegant solution for cutting through the foam samples to expose inner
surfaces while minimizing distortion of the foam. The authors (sorry, I don't
remember who they were...) impregnated the foam with isopropanol and then
cooled the specimen to make a composite that could be esily trimmed.
After trimming, the isopropanol was sublimed.

Best Regards,
John

John R. Minter, Ph. D. Phone: (716) 722-3407
Eastman Kodak Company FAX: (716) 477-3029
Analytical Technology Division email: minter-at-kodak.com
Rochester, NY 14562-3712






From: stoughma-at-ornl.gov (Matthew A. Stough)
Date: Fri, 30 Aug 1996 12:09:28 -0400 (EDT)
Subject: Subscribe Microscopy Matthew Stough

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Subscribe Microscopy Matthew Stough

(* In case this is not an automatic subscription cgi, please add me to the
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From: Robert Derby :      derby-at-pb.net
Date: Fri, 30 Aug 1996 12:41:55 -0500
Subject: Pixera

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Anyone have any experience with the Pixera Pro. CCD?
Pro's & Con's
Thanks in advance
Robert Derby





From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Fri, 30 Aug 1996 12:39:31 -0500
Subject: Re: ROTO

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Hi Karen, Ruthenium Red/Osmium/Tannic Acid/Osmium. This is an ionic stain
used for extracellular matrix whith the idea of stabilizing the stained
matrix against collapse during room temp. dehydration by covalent cross
bridging with osmium-tannic acid-osmium bridges. Careful washing between
each step avoids non-covalently bound osmium black precipitation which are
undesirable in TEM as well as in ultra-high resolution FSEM. Variations of
this method with heavy osmium black precipitation are used in some
preparation approaches in conventional SEM for the replacement of metal
(gold) coatings. Klaus
**************
} Has anyone heard of a contrast enhancing stain for TEM on
} biological sections called ROTO? If so, I'd like to know more about it.
} A reply to the list would be very helpfull.



******************************************************************************
* Klaus-Ruediger Peters, Ph.D. :Molecular Imaging Laboratory *
* Director, Molecular Imaging Laboratgory : http://panda.uchc.edu/ *
* Biomolecular Structure Analysis Center : htklaus/index.html *
* University of Connecticut Health Center : *
* 263 Farmington Ave. :F r e e Access to Differential *
* Farmington, CT 06030-2017; U.S.A :Contrast Software at *
* e-Mail: Peters-at-BSAC.UCHC.EDU : http://panda.uchc.edu/ *
* Tel: (860) 679-3977; Fax: (860) 679-1989 : htklaus/DHP-Img.html#Software*
******************************************************************************







From: Richard Lee :      richard_lee-at-QMGATE.ANL.GOV
Date: 30 Aug 1996 08:55:55 -0600
Subject: Microanalysis Services

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From: Terry.R.McCue-at-mcdermott.com
Date: 29 Aug 96 11:03:00 -0500
Subject: Microanalysis Services

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Colleagues

Our lab is looking for microanalysis services :

* Wish to analyze grain boundaries of Alloy 690 materials; objective
is to compare composition of grain boundary regions which exhibit
differences in metallographic etching response ( 2% Bromine-Methanol
Etchant).

* Interested in applying:

Main Interest - * SIMS imaging for element distribution

* APFIM atom probe field ion microscopy

* Analytical transmission electron microscopy of thinned foils.

** (Reference: MET & MATERIALS TRANSACTIONS A, Volume 27A, Feb. 1996,
page 327

We'd like to identify potential services vendors.

Need to define scope of the deliverable from each test and per
specimen cost.


Larry W. Sarver & Terry R. McCue
Metallurgical Analysis Section
Babcock & Wilcox Research
1562 Beeson St.
Alliance, Ohio 44601
(330) 829-7463 or 7427 Fax: (330) 829-7831
Internet: larry.w.sarver-at-mcdermott.com
terry.r.mccue-at-mcdermott.com

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From: d. kingston :      kingston-at-julian.uwo.ca
Date: Fri, 30 Aug 1996 16:08:05 -0400 (EDT)
Subject: Unsubscribe

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From: scott.wight-at-nist.gov (Scott Wight)
Date: Fri, 30 Aug 1996 12:22:10 -0500
Subject: Re: stereololgy tool

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You can make yourself an analog version of the tool you describe with a
washer (wheel), nut, machine screw, dowel with hole drilled near the end to
fit machine screw. I just made one in 5 minutes and it works quite well.
Take a permanent marker and make a mark on the side of the washer for
start/stop, attach washer to dowel with machine screw - not too tight it
has to roll. Calibrate by running the washer down a ruler, divide
revolutions into distance travled to get the travel of one revolution of
the washer. Now you are ready to measure by counting revolutions. A
machinist could make a better wheel with a known circumference, even press
in a bearing if you wanted to get fancy. At that point it will be cheaper
to buy a commercial map wheel.

} I need a tool (non-computer) that will allow me to trace a shape and get some
} kind of distance travelled measurement. I remember a special pen that had a
} rollerball in the tip with a digital readout on the shaft, but I cannot locate
} one or even a catalog with anything similar. Please help!!! Any product with
} a similar ability would also work.
}
} TIA
}
} Eugene Krueger
} GI Research
} Mayo Foundation
} krueger.eugene-at-mayo.edu

Scott Wight e-mail: SCOTT.WIGHT-at-NIST.GOV
NIST - Microanalysis Group W voice: 301-975-3949
Bld 222, Rm A113 | fax: 301-216-1134 or 301-417-1321
Gaithersburg, MD 20899 \|/ disclaimer:
Any opinion expressed is my own and does not represent those of my employer.
Web Page: http://www-sims.nist.gov/Division/MicroGroup.html






From: Stine Kraeft :      kraeft-at-mbcrr.harvard.edu
Date: Fri, 30 Aug 1996 16:18:40 -0400
Subject: Re: stereololgy tool

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From: Gary Login :      glogin-at-bih.harvard.edu
Date: Fri, 30 Aug 1996 12:54:19 -0400
Subject: Re: Microwave #2

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Dear Greg: Thanks for your query- I list my comments and references
below each question. I think the issues are general enough in nature
that a posting to the news group members interested in microwave
methods will be beneficial:



} Question Set TWO:

} Our Lab is currently investigating the possibilities of incorporating

} microwave technology in Electron Microscopy. Would be interested in
hearing

} from other EM labs using microwave processing for biological
applications

} regarding the pro=EDs and cons.


Well controlled microwave conditions will enhance penetration of
chemical fixatives within seconds. The following books are available
in libraries and should be helpful for this application. Please
contact me if you would like additional information.


Text Books on Microwave Fixation

1. Kok LP, Boon ME. Microwave Cookbook for Microscopists (Coulomb
Press, Leyden, 1992).


2. Login GR, Dvorak AM. The Microwave Toolbook. A Practical Guide for
Microscopists (Beth Israel Hospital, Boston, 1994).


3. Login GR, Dvorak AM. Methods of microwave fixation for microscopy. A
review of research and clinical applications: 1970-1992. Prog Histochem
Cytochem 1994;27/4: 1-127.



} Does anyone have any methods for "processing" in the MW

} i.e. times, temp, etc. for buffer washes, dehydration (acetone or
alcohol),

} infiltration schedules for biological applications, (liver, kidney,
spleen).


General methods collected from the literature are compiled in the book:
Kok LP, Boon ME. Microwave Cookbook for Microscopists (Coulomb Press,
Leyden, 1992)- Specific methods will need to be calibrated in your
oven.


References on microwave processing:

1. L.P. Kok, P.E. Visser and M.E. Boon, "Histoprocessing with the
microwave oven: an update," Histochem J, 20, 323-328 (1988).

2. A.S.-Y. Leong, "Microwave fixation and rapid processing in a large
throughput histopathology laboratory," Pathol, 23, 271-273 (1991).

3. D. Hopwood, "Microwaves and tissue processing," USA Microsc
Analysis, 1, 23-25 (1993).

4. L.P. Kok and M.E. Boon, "Ultrarapid vacuum-microwave
histoprocessing," Histochem J, 27, 411-419 (1995).



} What is the better mode for fixation, time or temperature ?, i.e. time
of

} fixation in the MW vs. change in temperature of fixative in MW, or a

} combination.


Both parameters must be carefully controlled for reproducible results.=20
I refer you to The Microwave Toolbook. A Practical Guide for
Microscopists (Beth Israel Hospital, Boston, 1994) Chapter 4 (Measuring
Temperature) =20

and Chapter 7 (Even Heating) for simple methods to identify the best
combination of parameters for your applications.



} Is there any way to increase the oven capacity (total number of
blocks/day or

} blocks/run?


Yes, use containers which are flat and which have a maximum depth of 1
cm because microwave energy (generated in laboratory microwave ovens)
can only penetrate ~1 cm into an aqueous medium (Do not stack
specimens). Using a large beaker of processing solution in a microwave
oven is counterproductive because samples which are more central in the
container are heated by conduction (contact with hot fluid) and not by
microwave energy. Of course if your samples are small enough (~1 mm3)
then conductive heat (via a hot plate) is just as effective as using a
microwave oven for rapid processing. See the article by Leonard JB,
Shepardson SP. Comparison of microwave and convective heating in rapid
specimen preparation techniques for electron microscopy. IEEE Internat
Microwave Symp Digest 1992.


} Some references state the importance of CACl2 or MgCl2 for fixation
and post

} fixation, any thoughts?


This is reviewed in Login GR, Dvorak AM. Methods of microwave fixation
for microscopy. A review of research and clinical applications:
1970-1992. Prog Histochem Cytochem 1994;27/4: 1-127.


0.75% calcium chloride and 2 mM magnesium chloride reportedly improve
morphology (Login GR, Dvorak AM. A review of rapid microwave fixation
technology: its expanding niche in morphologic studies. Scanning
1993;15:58-66), and antigen immunoreactivity (Ohtani H.
Microwave-stimulated fixation for preembedding immunoelectron
microscopy. Eur J Morphol 1991;29:64-67.). 1-10 mM Zinc Chloride has
also been used with success (Login GR, Dvorak AM. Prog Histochem
Cytochem 1994;27/4: 1-127)




} Question Set ONE:

} Our Lab is currently investigating the possibilities of incorporating

} microwave technology in Electron Microscopy. Would be interested in
hearing

} from other EM labs using microwave processing for biological
applications

} regarding the pros and cons.


Most microwave recipies are tailored for a specific microwave oven and
usually result in disappointing results when used in a different
microwave device. The most recent microwave methods literature
emphasizes calibration of a microwave oven to facilitate adapting
published recipies to your equipment. So, if at first you don't
succeed please let me know- we can discuss the protocol in more detail.
=20


} Some questions I have at the moment are:

} How do you en bloc stain with uranyl acetate, what percent, how much
time to

} microwave exposure, and the ideal staining temperature in the MW oven


I recommend that you begin by following the protocol published for
osmium tetroxide (it describes appropriate safety measures for handling
heavy metal fixatives): Login GR, Ku T-C, Dvorak AM. Rapid primary
microwave-aldehyde and microwave-osmium fixation. Improved detection
of rat parotid acinar cell secretory granule a-amylase using a
postembedding immunogold ultrastructural morphometric analysis. J
Histochem Cytochem 1995;43:525-533.



}

} Polymerizing EM blocks in the MW. Has anyone found a preference for
using a

} water load? Any success with Embed 812 kit, were there any NMA
problems.=20


There are two approaches in the literature for microwave accelerated
curing of resins: 1} Giammara's approach for flat embedding molds and
2} Giberson and Demaree's approach for Beam Capsules.


My suggestion for flat embedding molds is to start with 50% power for
15 minutes (100% will definately give you disappointing results). Also
when using flat embedding molds, allow your blocks to cool for 15
minutes before removing them from their molds, and vent your microwave
oven during curing.


I highly recommend using an appropriately sized water load for your
oven during microwave curing in flat embedding molds (otherwise there
is simply too much energy in a microwave oven and specimen damage from
over heating will result). In addition, microwave curing in an
uncalibrated microwave oven is very tricky and usually results in
disappointing results (e.g., incomplete curing of blocks). Simple
tools that you can make and a detailed description of how to use them
to calibrate your microwave oven are published in the The Microwave
Tool Book.



When curing resins in BEEM capsules, they are placed IN a water bath-
temperature never exceeds 100 =B0C and curing times are ~ 75 minutes. =20
See the reference by Giberson RT, Demaree RS, Jr: Microwave fixation:
understanding the variables to achieve rapid reproducible results.
Microsc Res Tech 32:246, 1995


} Any preference with EMBED 812/Araldite 502, if so the time and power,
i.e. 15

} min 50% power using a 700 watt MW oven.


Detailed information regarding curing times of various resins in a
microwave device can be found in


1. Giammara B. Microwave embedment for light and electron microscopy
using Epoxy resins, LR White, and other polymers. Scanning
1993;15:82-87.


2. Login GR, Dvorak AM. The Microwave Toolbook. A Practical Guide for
Microscopists (Beth Israel Hospital, Boston, 1994). The book contains a
table of curing times for resins tested.


Please contact me if you have additional questions and I will respond
directly to you.






Dr. Gary R. Login

Dept. Pathology

Beth Israel Hospital

330 Brookline Avenue

Boston, MA 02215


phone: 617-667-2034

fax: 617-667-8676


e-mail: glogin-at-bih.harvard.edu





From: Stine Kraeft :      kraeft-at-mbcrr.harvard.edu
Date: Fri, 30 Aug 1996 16:18:05 -0400
Subject: Re: Image networking from PC to MAC

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quit




From: lmaser-at-assnmgtinc.com (Larry Maser)
Date: Fri, 30 Aug 1996 15:52:19 GMT
Subject: Re: Fall 1996 - TEM Course Announcement

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Dear Stephen,

Might the students be interested in membership in the Microscopy Society of
America? May I send to you or someone a batch of brochures (10?) promoting
the Society? Hoping for a positive reply,

Larry Maser
Meeting and Business Office Manager for the Microscopy Society of America

At 11:04 AM 8/1/96 -0500, Steve Beck wrote:
} FALL 1996 COURSE ANNOUNCEMENT - Transmission Electron Microscopy (BIO. 221-E2)
}
} NASSAU COMMUNITY COLLEGE
}
} A fifteen week, fall 1996 semester, course in Biological Transmission
} Electron Microscopy is being offered by the Biology Department of Nassau
} Community College. This is a 4 credit course offered ONE EVENING PER WEEK,
} Thursdays, starting at 5:30pm. Classes will begin on Sept. 5 and end on
} Dec. 12, 1996.
}
} This is a "hands-on" course emphasizing biological specimen preparation,
} ultra-thin sectioning involving block trimming, glass knifemaking and
} operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III),
} thick and ultra-thin section mounting and contrast staining (UA and Pb
} citrate), grid support films (formvar, carbon), student operation of the
} TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs
} through the process of black & white photography, and electron micrograph
} analysis. Students will work on a chosen sample(s) with the goal of
} producing a portfolio of high quality TEM photomicrographs of that
} sample(s).
}
} The course is widely transferrable and the cost per credit is reasonable at
} $78 per credit.
}
} More information about the Bio-Imaging Center at NCC, course descriptions
} and syllabi, and the humble beginnings of a student gallery of EM
} photomicrographs is available at our recently completed web site. The URL
} is {http://www.sunynassau.edu/webpages/biology/becks.htm} . Any comments or
} suggestions on the homepage would be appreciated - I'm somewhat new at
} this!
}
} For those without www access, the catalog description is specified below.
} If you have further questions, you should e-mail me directly at the address
} below.
}
} Interested individuals should register early (prior to Aug. 15) since the
} course is limited to a total enrollment of ten (10) students.
} ____________________________________________________________________________
} ____
}
} CATALOG DESCRIPTION
} BIO 221: Transmission Electron Microscopy -- 4 credits
} Prerequisites: BIO 109-110 or equivalent, CHE 151-152 or equivalent.
} An introduction to the basic principles of transmission electron
} microscopy including tissue preparation, microscope (TEM) operation, black
} & white photography, and micrograph interpretation. The entire laboratory
} is devoted to the development of skills and preparative techniques involved
} with the operation of an actual transmission electron microscope.
} (3 lecture, 3 laboratory hours). Laboratory fee applies.
} ___________________________________________________________________________
_____
}
}
}
}
}
}
} Stephen J. Beck
} Bio-Imaging Center/Electron Microscopy
} Department of Biology
} Nassau Community College
} Garden City, NY 11530
} Voice Mail: (516) 572-7829
} Email: {becks-at-sunynassau.edu}
} URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}
}
}
}





From: lmaser-at-assnmgtinc.com (Larry Maser)
Date: Fri, 30 Aug 1996 15:52:19 GMT
Subject: Re: Fall 1996 - TEM Course Announcement

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear Stephen,

Might the students be interested in membership in the Microscopy Society of
America? May I send to you or someone a batch of brochures (10?) promoting
the Society? Hoping for a positive reply,

Larry Maser
Meeting and Business Office Manager for the Microscopy Society of America

At 11:04 AM 8/1/96 -0500, Steve Beck wrote:
} FALL 1996 COURSE ANNOUNCEMENT - Transmission Electron Microscopy (BIO. 221-E2)
}
} NASSAU COMMUNITY COLLEGE
}
} A fifteen week, fall 1996 semester, course in Biological Transmission
} Electron Microscopy is being offered by the Biology Department of Nassau
} Community College. This is a 4 credit course offered ONE EVENING PER WEEK,
} Thursdays, starting at 5:30pm. Classes will begin on Sept. 5 and end on
} Dec. 12, 1996.
}
} This is a "hands-on" course emphasizing biological specimen preparation,
} ultra-thin sectioning involving block trimming, glass knifemaking and
} operation of the ultramicrotomes (Sorvall MT-2B and LKB Ultrotome III),
} thick and ultra-thin section mounting and contrast staining (UA and Pb
} citrate), grid support films (formvar, carbon), student operation of the
} TEM (Hitachi HS-8, Philips EM 300) and production of electron micrographs
} through the process of black & white photography, and electron micrograph
} analysis. Students will work on a chosen sample(s) with the goal of
} producing a portfolio of high quality TEM photomicrographs of that
} sample(s).
}
} The course is widely transferrable and the cost per credit is reasonable at
} $78 per credit.
}
} More information about the Bio-Imaging Center at NCC, course descriptions
} and syllabi, and the humble beginnings of a student gallery of EM
} photomicrographs is available at our recently completed web site. The URL
} is {http://www.sunynassau.edu/webpages/biology/becks.htm} . Any comments or
} suggestions on the homepage would be appreciated - I'm somewhat new at
} this!
}
} For those without www access, the catalog description is specified below.
} If you have further questions, you should e-mail me directly at the address
} below.
}
} Interested individuals should register early (prior to Aug. 15) since the
} course is limited to a total enrollment of ten (10) students.
} ____________________________________________________________________________
} ____
}
} CATALOG DESCRIPTION
} BIO 221: Transmission Electron Microscopy -- 4 credits
} Prerequisites: BIO 109-110 or equivalent, CHE 151-152 or equivalent.
} An introduction to the basic principles of transmission electron
} microscopy including tissue preparation, microscope (TEM) operation, black
} & white photography, and micrograph interpretation. The entire laboratory
} is devoted to the development of skills and preparative techniques involved
} with the operation of an actual transmission electron microscope.
} (3 lecture, 3 laboratory hours). Laboratory fee applies.
} ___________________________________________________________________________
_____
}
}
}
}
}
}
} Stephen J. Beck
} Bio-Imaging Center/Electron Microscopy
} Department of Biology
} Nassau Community College
} Garden City, NY 11530
} Voice Mail: (516) 572-7829
} Email: {becks-at-sunynassau.edu}
} URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}
}
}
}





From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Sat, 31 Aug 1996 04:15:01 -0500
Subject: trans-Golgi antibodies

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I am looking for a commercially available trans-Golgi antibody for
immunoctyochemistry. Does anybody know of one? TIA









From: y78-at-ornl.gov (Sharon Robinson)
Date: Fri, 30 Aug 1996 16:03:09 -0400 (EDT)
Subject: email list

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I would to be added to the microscopy mailing list.

Thank you

Sharon Robinson
y78-at-ornl.gov






From: INGEBORG PAULUZZI :      pauluzzi-at-rsbs-central.anu.edu.au
Date: Sat, 31 Aug 1996 15:58:25 EST10
Subject: unsubscribe

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From: zaluzec-at-Sparc5.Microscopy.Com (Nestor J. Zaluzec)
Date: Sat, 31 Aug 1996 12:52:19 -0500
Subject: Materials Science EM PostDoc Position in Brazil

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RESEARCH ASSOCIATE and POST-DOCTORAL positions

Available in Rio de Janeiro - Brazil

Please contact and mail your resume to:

Prof. Guillermo Solorzano
President
Brazilian Society for Electron Microscopy
C.P. 38090 - Gavea
22452 Rio de Janeiro
Brazil

Fax: +5521-5112489/5112196
e-mail: SBME-at-RDC.PUC-RIO.BR



Requirements; Ph.D. in Materials Science and Engineering or Physics with
hands-on expenence in experimental AEM and HREM of metalic and ceramic
materials. Highly desired expenence with Jeol 2010 TEM and with image
processing operations.

Task; Co-ordinate preparation of bulk and cross-section samples. Operate
(and give demostrations to students) a Jeol 2010 TEM and a Zeiss 960 SEM.
Strong and interactive participation in research projects involving
detailed microstructural characterization of engineering materials and
minerals.

Salary; According with experience, comparable to equivalent position in the
US. Round tnp airplane ticket provided. One year or two years appointments
with possibility of renewal.

Please contact and mail your resume to:

Prof. Guillermo Solorzano
President
Brazilian Society for Electron Microscopy
C.P. 38090 - Gavea
22452 Rio de Janeiro
Brazil

Fax: +5521-5112489/5112196
e-mail: SBME-at-RDC.PUC-RIO.BR






From: lanier-at-slip.net (Dr. Wayne Lanier)
Date: Sun, 25 Aug 1996 13:20:48 -0700
Subject: Re: Plans for Light Boxes?

Contents Retrieved from Microscopy Listserver Archives
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Thanks to all who contributed ideas about home-made light boxes. Attached
is my personal favorite.

Bob Wise

***********

I used an old wine box.

Out here in sunny California* three or four-bottle wooden gift wine boxes
are common and empty ones can be obtained from liquor shops for about
$2.50. These boxes are about 10" X 12" X 6" and have a sliding top made of
0.25" wood, usually stamped or printed with the wine makers logo. Inside
are two or three dividers or, sometimes, cut out bottle holders.

I tossed the sliding top and replaced it with an 0.25" sheet of translucent
lucite plastic. I happended to have it on hand, but these can be gotten
from any plastics supplies dealer. Usually they will give you scrap of
roughly the right size. The plastic I had on hand was clear, so I sanded
it to translucency. If you buy a sheet you can get a better quality of
translucent plastic.

The box I used had two wood dividers inside (a 3-bottle box). I left them
in and covered them and the bottom with a sheet of aluminum foil over
cardboard to make a kind of curvy "W"-shaped reflecting surface.

Then I tucked three small fluorescent lights into the box, added a switch
and a hardware store handle, and Presto! Light box.

*where I live, San Francisco, we've had fog and 60-deg weather for the last
month.


Dr. Wayne Lanier
250 Ashbury
San Francisco, CA 94117
TEL: [415] 346-4940
e-mail -at-home: lanier-at-slip.net
e-mail -at-work: lanier-at-resbiom.com








From: Andreas Brech :      andreas.brech-at-bio.uio.no
Date: Sun, 01 Sep 1996 12:09:07 +0100
Subject: answer

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Message-Id: {199609021014.MAA00714-at-darwin.uio.no}
X-Sender: abrech-at-darwin.uio.no
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recieved message correct





From: csbeneas :      csbeneas-at-wiccmail.weizmann.ac.il
Date: 2 Sep 1996 17:50:26 +0200
Subject: cryo jet

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Message-ID: {n1370435812.81260-at-wiccmail.weizmann.ac.il}

Hi,
I'm looking for a cryo jet commercialy avaliable or used, I'll be very glad to
get any information concerning to this, thank you in advance, Elia





From: ueksi-at-eos.ncsu.edu
Date: Mon, 2 Sep 1996 13:53:03 -0400 (EDT)
Subject: Silicon Chip Images ?

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I am looking for magnified images of silicon chips. Does anybody know
where to find such stuff ?

__________________________________________________________________________
Umut Eksi Phone +1 919 512-5169
400-B Watauga Hall
Box 21675, NCSU ueksi-at-eos.ncsu.edu
Raleigh, NC 27607, USA http://www4.ncsu.edu/unity/users/u/ueksi/www
__________________________________________________________________________




From: Andrews Lab :      Andrews.Lab-at-quickmail.yale.edu
Date: 2 Sep 1996 15:06:35 -0400
Subject: unsubscribe

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Message-Id: {n1370445471.78414-at-QuickMail.Yale.edu}

please unsubscribe andrews_lab-at-quickmail.yale.edu
thank you





From: Zaluzec-at-aaem.amc.anl.gov (Nestor J. Zaluzec)
Date: Mon, 2 Sep 1996 12:26:44 -0600
Subject: Looking for UK Parts Supplier Info

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G'day Colleagues...

I'm doing a repair job on my Philips 501 SEM system
(no service contract) and need to locate a supplier
of the pneumatic pressure valves.

The valves are actually working, the problem is that
the small plungers which do the sealing need replacing.
These items can't cost more than $10-20, and replacing the
whole valve ~$100 is a bit excessive and overkill.

If any of my UK colleagues can locate a phone/fax/email
address for me for the following company I would appreciate
it.

MEAD FLUID DYNAMICS
ABEX MEAD Ltd

Pneumatic Valve # P05-150-516 14/93



They may be located in Burgess Hill area, but I
cannot be sure as the valves on the system are
pretty old.


Thanks in advance

Nestor
Your Friendly Neighborhood SysOp






From: bcockman-at-uniwa.uwa.edu.au (Brett Cockman)
Date: Tue, 3 Sep 1996 18:01:28 +0800
Subject: Vorticellapreparation method

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Message-ID: {199609030151.UAA04837-at-IndyNet.indy.net}
To: Mike Nicksic {menco-at-azstarnet.com} ,
Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

Hello fello microscopists,

I need a reliable method for preparing Vorticella slides. I would be most
grateful if someone has a good method for making permanent preparations of
these delicate protists and would not mind sharing it.
I look forward to your response.
Thanks

Brett








From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Tue, 3 Sep 1996 07:57:23 -0500
Subject: Re: Web Sources on Microscopy Sellers/Buyers

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Message-Id: {v03007802ae51db92bc7e-at-[206.69.208.21]}
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Another list of Commerical Microscopy & Microanalysis
Web Sites can be found at:

http://www.amc.anl.gov/Docs/NonANL/ComSites.html

I have just recently updated that list so there are
additional firms that buy and sell new and used
instrumentation. (Annotations about the various
companies will slowly be added). The master lists
of EDU, GOV, COM, and Microscopy Society sites can be accessed
via the M&M Home Page.

http://www.amc.anl.gov

As usual if your WWW site is missing just send a me a off-line
note at:
Zaluzec-at-AAEM.AMC.ANL.GOV
or
Zaluzec-at-Microscopy.Com



Cheers....

Nestor
Your Friendly Neighborhood SysOp






From: csedax-at-alpha.arcride.edu.ar
Date: Tue, 3 Sep 1996 10:41:37 -2036
Subject: SEM: Tensile and Torsion testing in different kinds of materials.

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Hi everyone,


I am a student from Argentina, and I am interested in the study of materials and the
next items: their mechanical properties, tensile and torsion test in diferents kinds of
materials like metals, polymers, ceramics, etc.. All this items refer to use of the
scanning electron microscopy.

If any of you know references about this subjet or names of people working on this
please send me a e-mail message if possible. I appreciate very much your attention,
thanks a lot.


Daniel F. Imbert

Centro Regional de Investigacion
y Desarrollo de Santa Fe.
Guemes 3450 - 3000 Santa Fe.
Argentina.






From: Braun :      braun-at-argos.ipfdd.de
Date: Tue, 03 Sep 1996 18:20:55 +0000
Subject: Magnetic beads

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Message-Id: {322C7707.61E5-at-argos.ipfdd.de}

Hello microscopists ,

I am looking for suppliers of magnetic beads ( preferently in Europe )

Who can help me ?

Thanks Hans-Georg Braun




From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 3 Sep 1996 11:21:10 -0700
Subject: Shared use of diamond knives?

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Hi:

I am trying to figure out how to let lab users share diamond knives
provided by me. If you have an opinion on the shared use of diamond knives,
I would be interested in it.

Here is the setup: I oversee a small EM lab, most of our TEM users are
undergraduate and graduate students. The glory of molecular biology has
turned the heads of most of our faculty so the level of support for student
TEM is fairly low. However, we do get students and other users who want to
try a project that requires sectioning. As long as there is minimal cost,
many students get started and try sectioning using glass knives.
Eventually, they run into the limitations of glass knives and want to use a
diamond knife.

When I suggest that they purchase a diamond knife, the wind goes out of
their sails. Most say they cannot afford even to have an old knife from
their lab resharpened, let alone afford to buy a new knife. I would like
to help them but feel burdened by the problem of how to distribute diamond
knife privileges among semi-beginning users.

To add to the current problem, I have several old diamond knives that need
resharpening. Letting users have access to these knives in their current
condition is not much help. It will cost us several thousand dollars to get
them resharpened. I think we have some money in the lab budget that could
be used for sharpening. Of course once they are sharpened, do I want to
pass them out to multiple users?

So it boils down to something like this:

I have users who need access to a diamond knife to do their work. They say
they are too poor to afford a knife. I have knives and money to sharpen
them. Do I play the Sugar Daddy and sharpen the knives and just let them
use them? Do you have any sage advice about establishing a policy to help
these paupers get their work done?

I struggle with the questions of how to make users borrowing an expensive
knife responsible so they are careful and can contribute to replacing the
knife if they damage it. I wonder about the need to set up some kind of
charge to use the knife when for most practical purposes any single user
will (hopefully) leave it as good as new. I wonder if it would be OK to let
more than one person use a knife at the same time. It goes on and on.

If you have worked on this problem or just have some personal insights to
help me I would appreciate hearing from you.




Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
FAX (408) 429-0146
jmkrupp-at-cats.ucsc.edu






From: Xianying Burany :      xianying-at-udel.edu
Date: Tue, 3 Sep 1996 16:03:59 -0400 (EDT)
Subject: EDS Detector Windows

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Dear All:

We have a ultra thin EDS detector window. It seems that it is too easy to
be broken. We need to analyze C (carbon) in our magnetic samples. Can you
tell me which type of EDS detector window I should choose.

Please reply directly to me.

I appreciate your help.

Sandy

Univ. of Delaware

94765-at-udel.edu or xianying-at-udel.edu





From: schooley-at-mcn.org (Caroline Schooley)
Date: Tue, 3 Sep 1996 16:38:25 -0700
Subject: Re: Silicon Chip Images ?

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Microscopy ListServer {Microscopy-at-Sparc5.Microscopy.Com}
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} I am looking for magnified images of silicon chips. Does anybody know
} where to find such stuff ?
}

} Umut Eksi
Look in Project MICRO's bibliography
(www.MSA.microscopy.com/ProjectMICRO/Books.html) under videotapes :
Marshall Space Flight Center. There is nice SEM of real-time voltage
contrast.

Caroline Schooley
Educational Outreach Coordinator, Microscopy Society of America
Box 117
Caspar, CA 95420
Phone/FAX: (707)964-9460
Email: schooley-at-mcn.org
Web: http://www.MSA.microscopy.com/Projrct MICRO/Books.html






From: Tom Donnelly :      tdonne-at-api.com
Date: Tue, 3 Sep 1996 16:22:22 -0700
Subject: New address for API

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================= commercial posting ==========================

Applied Precision is moving! We have outgrown our current location on Mercer
Island, and are moving to a new location in Issaquah, Washington. Our new
building is located a few miles outside of Seattle, and should provide room
to grow for many years.

Our new address:

Applied Precision, Inc.
1040 12th Avenue NW
Issaquah, WA 98027-8929
Phone: (206)557-1000
Fax: (206)557-1055

Our email and WWW site domain name will remain the same: api.com (or
http://www.api.com).

Regards,

Tom Donnelly


Tom Donnelly 206-313-4549 tel
DeltaVision Systems 206-557-1055-4184 fax
Applied Precision, Inc. tdonne-at-api.com
1040 12th Ave. N.W. Web Site: http://www.api.com
Issaquah, WA 98027-8929







From: B.Pirie-at-unsw.edu.au (Brian Pirie)
Date: Wed, 4 Sep 1996 12:07:23 +1000
Subject: UNICRYL

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DOES ANYBODY HAVE ANY EXPERIENCE USING UNICRYL(By BioCell).WE ARE HAVING
DIFFICULTIES POLYMERIZING IT.HOW INTENTION IS TO USE IT FOR E.M & L.M.
THANKS IN ADVANCE
BRIAN






From: drouillon-solvay-at-e-mail.com
Date: Wed, 04 Sep 1996 02:54:49 EDT
Subject: RE : SEM: TENSILE AND TORSION TESTING IN DIFFEREN

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Hello Daniel,


Georg H. Michler has published a review with 37 references about the
investigation in situ of the micromechanical mechanisms in polymers by electron
microsocpy (TEM and SEM).
The reference follows :
"Electron Microscopic Techniques for Direct Investigation of Micromechanical
Mechanisms in Polymers". Georg H. Michler, TRIP, vol.3, n4, April 1995,
pp 124-131


Best regards


Philippe Drouillon
Solvay Research & Technology
Electron Microscopy Unit

Extra X400 information begins:
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From: jiangj-at-Papin.HRZ.Uni-Marburg.DE
Date: Wed, 4 Sep 1996 12:46:27 +0000
Subject: subscribe

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Message-Id: {9609041046.AA35290-at-Mailer.Uni-Marburg.DE}
Comments: Authenticated sender is {jiangj-at-mailer.uni-marburg.de}

Dr. Jiechao Jiang
Fachbereich Geowissenschaften
Philipps Universitaet Marburg
Hans-Meerwein-Strasse
D-35043 Marburg
Germany

Tel: +49 6421 283458
Fax: +49 6421 288919

email: jiangj-at-mailer.uni-marburg.de




From: mlamvik-at-mcnc.org
Date: Wed, 4 Sep 1996 07:42:38 -0400
Subject: Preparing Ta for TEM

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I'm trying to thin a cross-section of tantalum by mechanical lapping and
polishing, and it's going very slowly. Does anyone have advice on how best
to do this? Suggestions will be appreciated.
Thanks,
Mike Lamvik






From: jiangj-at-Papin.HRZ.Uni-Marburg.DE (Jiang Jiechao Dr. )
Date: Wed, 4 Sep 1996 14:38:53 +0200
Subject: subscribe

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From: rgrappe-at-MMM.COM
Date: Wed, 04 Sep 1996 08:40:15 -0500
Subject: LM - Image Analysis

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I would like to locate a simple image processing program that runs on
Windows 3.1. All I want is a program which let me set calibrations for
various objectives and make length measurements and possibly do some
contrast enhancements.
Thanks for your help.

Rod Rappe
Imation Corporation
RGRappe-at-MMM.com




From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Wed, 04 Sep 1996 08:38:04 -0400 (EDT)
Subject: Re: Shared use of diamond knives?

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The few students that I have in my lab all use a single diamond knife. Of
course they start out with a glass knife and when I feel that they can handle
a diamond, I let them use it. Most labs nowadays need to be user friendly and
this usually means sharing our most prized possesions, so in the long run, let
the folks use a diamond.

Cheers,
Ed Calomeni
Medical College of Ohio
Toledo, OH
emlab-at-opus.mco.edu




From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Wed, 4 Sep 1996 09:30:34 -0400 (EDT)
Subject: Re: UNICRYL

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Dear Brian,

I do not have experience with Unicryl, but they are considering its use in
our lab and I am very skeptical. Would you please pass on the info you
receive to all of us on the listserver?

Thank you
Cheri Owen
Detroit Neurotrauma Institute
Wayne STate University
Detroit, Mi

On Wed, 4 Sep 1996, Brian Pirie wrote:

} DOES ANYBODY HAVE ANY EXPERIENCE USING UNICRYL(By BioCell).WE ARE HAVING
} DIFFICULTIES POLYMERIZING IT.HOW INTENTION IS TO USE IT FOR E.M & L.M.
} THANKS IN ADVANCE
} BRIAN
}
}
}





From: drouillon-solvay-at-e-mail.com
Date: Wed, 04 Sep 1996 06:26:56 EDT
Subject: RE : SEM: TENSILE AND TORSION TESTING IN DIFFEREN

Contents Retrieved from Microscopy Listserver Archives
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Hello Daniel,


Georg H. Michler has published a review with 37 references about the
investigation in situ of the micromechanical mechanisms in polymers by electron
microsocpy (TEM and SEM).
The reference follows :
"Electron Microscopic Techniques for Direct Investigation of Micromechanical
Mechanisms in Polymers". Georg H. Michler, TRIP, vol.3, n4, April 1995,
pp 124-131


Best regards


Philippe Drouillon
Solvay Research & Technology
Electron Microscopy Unit

Extra X400 information begins:
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From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Wed, 4 Sep 1996 08:01:50 -0700 (PDT)
Subject: Re: UNICRYL

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On Wed, 4 Sep 1996, Brian Pirie wrote:

} DOES ANYBODY HAVE ANY EXPERIENCE USING UNICRYL(By BioCell).WE ARE HAVING
} DIFFICULTIES POLYMERIZING IT.HOW INTENTION IS TO USE IT FOR E.M & L.M.
} THANKS IN ADVANCE
} BRIAN
}
}
}
Hello Brian,

We have been experimenting with unicryl in search of of a easy reliable
way of doing post embedded immunohistochemistry. We were using LR White
for years and still haven't totally switched to unicryl. However we have
had some good results.

If the tissue isn't polymerized it may be infiltration or getting all the
alcohol out. If the whole block isn't polymerizing, I have found that the
polymerization times in the booklet are way too short. I have had to
polymerize under UV for a week or more and never had a block polymerize
under 4 days. We have also discovered and heard from others that it is
impossible to over polymerize.

We keep the resin in alliquots at -20, letting it warm completely befor
use. We go directly from 100% EtOH to resin
resin 2X 1hr
resin overnite
resin 2X 1hr
orient tissue in polyethelene molds uncovered or beem capsules covered or
permanox tissue culture dishes sealed.
UV chamber with ice packs about 8 degrees for 2-3 days then let
temperature come up to room temp for the remainder.

We still don't have all the bugs worked out but hope this helps.

Bob Underwood
Morphology Core
University of Washington






From: Leo Marin :      leo-at-spine.med.utoronto.ca
Date: Wed, 4 Sep 1996 09:25:51 -0400 (EDT)
Subject: Re: Shared use of diamond knives?

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Hi,
Would it not be possible to assign a diamond knife to a limited number of
students and have the cost shared by the supervisors and/or course budgets?

Leo

On Tue, 3 Sep 1996, Jon Krupp wrote:

} Hi:
}
} I am trying to figure out how to let lab users share diamond knives
} provided by me. If you have an opinion on the shared use of diamond knives,
} I would be interested in it.
}
} Here is the setup: I oversee a small EM lab, most of our TEM users are
} undergraduate and graduate students. The glory of molecular biology has
} turned the heads of most of our faculty so the level of support for student
} TEM is fairly low. However, we do get students and other users who want to
} try a project that requires sectioning. As long as there is minimal cost,
} many students get started and try sectioning using glass knives.
} Eventually, they run into the limitations of glass knives and want to use a
} diamond knife.
}
} When I suggest that they purchase a diamond knife, the wind goes out of
} their sails. Most say they cannot afford even to have an old knife from
} their lab resharpened, let alone afford to buy a new knife. I would like
} to help them but feel burdened by the problem of how to distribute diamond
} knife privileges among semi-beginning users.
}
} To add to the current problem, I have several old diamond knives that need
} resharpening. Letting users have access to these knives in their current
} condition is not much help. It will cost us several thousand dollars to get
} them resharpened. I think we have some money in the lab budget that could
} be used for sharpening. Of course once they are sharpened, do I want to
} pass them out to multiple users?
}
} So it boils down to something like this:
}
} I have users who need access to a diamond knife to do their work. They say
} they are too poor to afford a knife. I have knives and money to sharpen
} them. Do I play the Sugar Daddy and sharpen the knives and just let them
} use them? Do you have any sage advice about establishing a policy to help
} these paupers get their work done?
}
} I struggle with the questions of how to make users borrowing an expensive
} knife responsible so they are careful and can contribute to replacing the
} knife if they damage it. I wonder about the need to set up some kind of
} charge to use the knife when for most practical purposes any single user
} will (hopefully) leave it as good as new. I wonder if it would be OK to let
} more than one person use a knife at the same time. It goes on and on.
}
} If you have worked on this problem or just have some personal insights to
} help me I would appreciate hearing from you.
}
}
}
}
} Jonathan Krupp
} Microscopy and Imaging Lab
} University of California
} Santa Cruz, CA 95064
} (408) 459-2477
} FAX (408) 429-0146
} jmkrupp-at-cats.ucsc.edu
}
}
}




From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Wed, 4 Sep 1996 19:31:23 +0100 (BST)
Subject: Getting back on to the NesterNet

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Nester:

You won your pint of beer, I have lost the instructions of how to
subscrine and unsubscribe. Send these details at your leisure but in the
meantine please put Patrick Echlin "pe13-at-cus.cam.ac.uk" back ino the
system. There is another pint either at the Free Press in Cambridge or at
a bar of your own choosing in Cleveland

Patrick Echlin
Cambridge UK





From: Luc Nocente :      ln-at-noesisvision.com
Date: Wed, 04 Sep 1996 15:08:49 -0400
Subject: Re: LM - Image Analysis

Contents Retrieved from Microscopy Listserver Archives
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Try Visilog from Noesis Vision, we have a new low end version for 2,995.00
which will accomplish what you are looking for, only problem is that it will
run only on WIndows 95.


At 08:40 AM 9/4/96 -0500, you wrote:
} I would like to locate a simple image processing program that runs on
} Windows 3.1. All I want is a program which let me set calibrations for
} various objectives and make length measurements and possibly do some
} contrast enhancements.
} Thanks for your help.
}
} Rod Rappe
} Imation Corporation
} RGRappe-at-MMM.com
}
}

----------------------------------------------------------
---------------------------------------
Luc Nocente Tel: 514 345 1400
Noesis Vision Inc. Fax: 514 345 1575
e-mail: ln-at-noesisvision.com
6800 Cote de Liesse, Suite 200
St-Laurent, PQ
H4T 2A7,Canada

Visit our new web site at http://www.cam.org/~noesis
----------------------------------------------------------------------------
---------------------





From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 4 Sep 1996 15:58:41 -0500
Subject: DOS shareware

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Message-ID: {n1370268933.65334-at-msmail.tmc.tulane.edu}

Are there sites for shareware sources for these softwares. Please respond
directly to {fermin-at-tmc.tulane.ed}

1) Ted.Com, a DOS a straightfoward text editor I prefer to others including
note pad. Someone told me that there is reasonably decent upgrade out there
(even today I use the old version)?

2) Typing Tutor or any other similar share ware for practicing and increasing
typing speed (not for me)

*********************************************************************
*Cesar D. Fermin, Ph.D \|T|/ Fax (504) 587-7389 *
*Tulane Medical School /|M|\ Voice Mail (504) 584-2618 *
*Pathology/SL79 \|C|/ Secretary (504) 584-2436 *
*New Orleans La 70112-2699 /|*|\ Lab/Techn. (504) 584-2521 *
* {Fermin-at-tmc.tulane.edu} ----} Prof. Pathology & Otolaryngology *
*http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html*
*********************************************************************





From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Wed, 4 Sep 1996 13:15:08 -0600
Subject: Re: SEM conversion to digital image capture.

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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} We have a nine-year old SEM - a Hitachi S-800 - which we
} would like to convert to digital image capture. We would
} prefer to do this by buying a complete commercial package,
} rather than by putting together a system of our own.
}
} If you have had such a system installed on your SEM, please
} send me your experience and recommendations.

In addition to the SemiCaps and 4pi systems, which take control of the
beam, there is a passive capture system called SEMages. It is sold in the
US through Advanced Database Systems. They can be reached at
jhilton-at-rmii.com.

We have one of these systems on our Philips 505. It has a very simple user
interface that doesn't allow many options or problems. All users of the
scope who have started using it have stayed with it and abandoned Polaroid
film. One of the things I like about the passive system is that it leaves
all normal functions of the instrument in tact and uses the calibration and
micron bar from the SEM.

If you have further questions, don't hesitate to contact me. Each of the
systems mentioned above has its strong points and reasons for selection.
The situation where they will be placed will help determine which one you
would choose. (Normal disclaimer: I don't have financial interest in any
of these companies.)

John
chandler-at-lamar.ColoState.EDU






From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 3 Sep 1996 11:21:10 -0700
Subject: Shared use of diamond knives?

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Message-ID: {n1370289985.51838-at-sjdccd.cc.ca.us}

Dear John,

I have had to "share" diamonds in both research and training labs. When we
did it for researchers, we estimated the amount of times it would have to be
used before resharpening and calculated the cost per use. Obviously the first
time that is done, you have to be sure you get the reshrapening amount out of
it. We used a log book for each knife (I have had 10 diamonds for sign out in
my past jobs). They always started from the left side and didn't move over
until it got dull in that area. They had to draw how the edge appeared in the
log book when finished. When they turned it back in usually I had them have
it under a stereo scope with a cleaning stick in water, just in case they
didn't get it clean and I looked at it. You don't have to monitor it however
it tends to stay in better condition if you do - I have tried it both ways. I
always had the funds transferred BEFORE they started using the knife.
Even with students, when it is monitored it generally cuts lots of tissue
before it needs resharpening. For my students I insist on seeing good
sections on glass knives prior to their cutting on a diamond. I then have a
training diamond, which isn't in the best shape and we use strictly for
training. I have them cut on that, to be sure they know how to change the
height properly etc. and get sections in ribbons. This lets me know they know
how to trim the block and set the diamond knife up.
I personally like to have my own diamond for my own research HOWEVER for
people that cut for one project for something, loaner diamonds do work -
monitoring it seems to be the trick. It takes a bit more time, but not that
much and generally means more users get good use of the diamond.
Good Luck,
Judy M.

Judy Murphy, PhD
Microscopy Technology Center
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5600
e-mail: murphy-at-sjdccd.cc.ca.us
program web page: http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html

_______________________________________________________________________________

Hi:

I am trying to figure out how to let lab users share diamond knives
provided by me. If you have an opinion on the shared use of diamond knives,
I would be interested in it.

Here is the setup: I oversee a small EM lab, most of our TEM users are
undergraduate and graduate students. The glory of molecular biology has
turned the heads of most of our faculty so the level of support for student
TEM is fairly low. However, we do get students and other users who want to
try a project that requires sectioning. As long as there is minimal cost,
many students get started and try sectioning using glass knives.
Eventually, they run into the limitations of glass knives and want to use a
diamond knife.

When I suggest that they purchase a diamond knife, the wind goes out of
their sails. Most say they cannot afford even to have an old knife from
their lab resharpened, let alone afford to buy a new knife. I would like
to help them but feel burdened by the problem of how to distribute diamond
knife privileges among semi-beginning users.

To add to the current problem, I have several old diamond knives that need
resharpening. Letting users have access to these knives in their current
condition is not much help. It will cost us several thousand dollars to get
them resharpened. I think we have some money in the lab budget that could
be used for sharpening. Of course once they are sharpened, do I want to
pass them out to multiple users?

So it boils down to something like this:

I have users who need access to a diamond knife to do their work. They say
they are too poor to afford a knife. I have knives and money to sharpen
them. Do I play the Sugar Daddy and sharpen the knives and just let them
use them? Do you have any sage advice about establishing a policy to help
these paupers get their work done?

I struggle with the questions of how to make users borrowing an expensive
knife responsible so they are careful and can contribute to replacing the
knife if they damage it. I wonder about the need to set up some kind of
charge to use the knife when for most practical purposes any single user
will (hopefully) leave it as good as new. I wonder if it would be OK to let
more than one person use a knife at the same time. It goes on and on.

If you have worked on this problem or just have some personal insights to
help me I would appreciate hearing from you.




Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
FAX (408) 429-0146
jmkrupp-at-cats.ucsc.edu



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From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Wed, 4 Sep 1996 18:11:11 -0600
Subject: LM/TEM, resin chemistry, methacrylates

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Greetings,
I recently made up some K4M where I left out the crosslinker. I
was expecting that without the crosslinker this resin, like other mixtures
of methacrylates, would be extractable with acetone after sectioning. To my
surprise, the sections were not extracted at all with acetone.

Now, unlike other kinds of methacrylate that I have used, the K4M
is made with methacrylate monomers with hydroxyls on their side chains. Do
free radicals form at these hydroxyls and in this way effectively crosslink
the resin? Or, do the hydroxyls give the resin a chemical character that
makes it less soluble in acetone? If the latter is the case, can anyone
suggest a solvent that might successfully extract the K4M where the acetone
failed? I might mention that I am wanting to do immunocytochemistry after
extraction, so I would rather not go to a really extreme solvent.

Thanks in advance,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: Sara Miller :      saram-at-acpub.duke.edu
Date: Wed, 4 Sep 1996 19:36:37 -0400 (EDT)
Subject: Re: UNICRYL

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On Wed, 4 Sep 1996, Robert Underwood wrote:

} Date: Wed, 4 Sep 1996 08:01:50 -0700 (PDT)
} From: Robert Underwood {underwoo-at-u.washington.edu}
} To: Brian Pirie {B.Pirie-at-unsw.edu.au}
} Cc: Microscopy-at-Sparc5.Microscopy.Com
} Subject: Re: UNICRYL
}
}
}
} On Wed, 4 Sep 1996, Brian Pirie wrote:
}
} } DOES ANYBODY HAVE ANY EXPERIENCE USING UNICRYL(By BioCell).WE ARE HAVING
} } DIFFICULTIES POLYMERIZING IT.HOW INTENTION IS TO USE IT FOR E.M & L.M.
} } THANKS IN ADVANCE
} } BRIAN
} }
} }
} }
} Hello Brian,
}
} We have been experimenting with unicryl in search of of a easy reliable
} way of doing post embedded immunohistochemistry. We were using LR White
} for years and still haven't totally switched to unicryl. However we have
} had some good results.
}
} If the tissue isn't polymerized it may be infiltration or getting all the
} alcohol out. If the whole block isn't polymerizing, I have found that the
} polymerization times in the booklet are way too short. I have had to
} polymerize under UV for a week or more and never had a block polymerize
} under 4 days. We have also discovered and heard from others that it is
} impossible to over polymerize.
}
} We keep the resin in alliquots at -20, letting it warm completely befor
} use. We go directly from 100% EtOH to resin
} resin 2X 1hr
} resin overnite
} resin 2X 1hr
} orient tissue in polyethelene molds uncovered or beem capsules covered or
} permanox tissue culture dishes sealed.
} UV chamber with ice packs about 8 degrees for 2-3 days then let
} temperature come up to room temp for the remainder.
}
} We still don't have all the bugs worked out but hope this helps.
}
} Bob Underwood
} Morphology Core
} University of Washington
}
Hi,
Can you please enlighten me as to why you went to Unicryl with all
its inherent problems? Is there better staining/ultrastructure??? We
use L. R. Gold whch polymerizes overnight at -20 with uv light. What am
I missing?
Sara}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: tspires-at-uts.cc.utexas.edu (Tara Spires)
Date: Wed, 4 Sep 1996 13:51:12 -0500 (CDT)
Subject: Re: UNICRYL

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unsubscribe





From: Yeong Seok Kim :      iecco-at-nuri.net
Date: Thu, 05 Sep 1996 16:42:51 +0900
Subject: Plasma Etch equip.

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Message-Id: {1.5.4.32.19960905074251.0068c194-at-nuri.net}
X-Sender: iecco-at-nuri.net
X-Mailer: Windows Eudora Light Version 1.5.4 (32)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear Microscopist,

We are finding someone who knows of Plasma etch equip and can offer it to us.
Detail info will be highly appreciated.

thank you in advance,

Interface Engineering Co. in Korea
Yeong S. Kim
INTERFACE ENGINEERING CO. +82-2-400-2605(T) +82-2-406-0762(F)





From: Luc Nocente :      ln-at-noesisvision.com
Date: Thu, 5 Sep 1996 09:08:33 BST
Subject: Re: LM - Image Analysis

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http://ddsdx.uthscsa.edu/

Keith
---
Interface Analysis Centre, University of Bristol, Oldbury House,
121, St. Michael's Hill, Bristol, BS2 8BS, England
Telephone: +44 (0)117 925 5666 | Facsimile: +44 (0)117 925 5646 |
URL: http://www.phy.bris.ac.uk/research/iac/home.html






From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Thu, 05 Sep 1996 08:17:31 -0400 (EDT)
Subject: Re: Negative Staining Problems

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Dear Jerry,

HBsAg is usually found in serum in very high concentrations, therefor
a 1:10 dilution with PBS is usually sufficient to reduce background proteins.
I have always used 1% ammonium molybdate as the negative stain.

Best of Luck,
Ed Calomeni
Medical College of Ohio
Toledo, OH 43699
emlab-at-opus.mco.edu




From: John Best :      jbest-at-Sparc5.Microscopy.Com
Date: Thu, 05 Sep 1996 08:52:09 -0700
Subject: Hissing EDS detector

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Message-ID: {322EF729.18A4-at-vicon.net}

Hello All,

First let me thank all of those who replied to my question about
interfacing MAC's to PC's.

We have a different problem today. I've recently installed an EDS
detector on my SEM. After filling with LN2, allowing 24 hrs to cool,
then topping off, I powered up the analyzer. There is a symptom of a
problem, i.e. the count rate is high even with no xray source.

It's a pulsed optical type preamp, and a check of the test point on the
preamp revealed a +/- 1V sawtooth approx 10 mS in duration. The
documentation indicates that with no xrays present this ramp should
ascend very slowly.

While looking for the cause, I noticed that the detector is "hissing".
It's been 36 hrs from the LN2 top off, and the detector has used about
20% of the LN2. I don't think this a necessarily high usage rate, but
when listening near the top of the dewar, I can still hear something
hapenning in there!

What do you think? Contamination in the dewar? Ice? I'm thinking of
pouring out the LN2, blowing the detector out with Argon and refilling
it. Any suggestions? Is there a cleaning procedure?

Thank You most kindly in advance for any and all consideration of this
matter.

Regards,
John.


--
John W. Best ELMDAS Co. Email: jbest-at-vicon.net
P.O. Box 355, Alexandria, PA, USA 16611
Voice: 814-669-4474
WWW: http://www.vicon.net/~jbest






From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Tue, 3 Sep 1996 11:21:10 -0700
Subject: Shared use of diamond knives?

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X-Sender: zaluzec-at-microscopy.com
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Nestor J. Zaluzec)


Dear Jon

you mention that your students run into the difficulties of glass knives and
therefore want to use diamonds. I accept that there are some exceptionally
hard materials about that actually require diamond but most typical animal
and a lot of plant material can be cut better on a good glass knife. I
worked for over 5 years in a pharmaceutical company and we only ever
routinely used glass for all of our cutting.

If the difficulties are really due to the specimens then there is no choice
but to work out a method of sharing diamonds. Another alternative could be
to make glass knife making as easy as possible for instance if you are using
tape to seal your water baths try using plastic disposable water baths and a
proper wax heater because this makes it much easier to make a good knife
especially for students (we use LKB 'TRUF's and the appropriate hot plate
wax dispenser - LKB 2208 multiplate). If your glass knives aren't very good
is it the source of glass, the quality of knife maker or a need for service
or upgrade of knife maker?

If students still need to cut with diamond then I have always given them a
lot of supervision, particularly when setting up and cleaning/removing the
knife from the microtome. Then I have to assess individual ability, needs
and frequency of use to decide how much supervision will be needed. If you
can't give this level of supervision then maybe you should consider limiting
the sharing of the knives. It's much easier to instil in someone the need
for care of a diamond if they know they are the only one, at a particular
time, using it. They feel more responsible and can't blame anyone else.

Finally after all of the above you may just have to accept that most users
can't or won't pay and so the investments or losses may have to be borne by
you.

I hope that I haven't just told you stuff you already know and good luck
when you decide.

Malcolm Haswell
e.m. unit
University of Sunderland
U.K.
e-mail: es0mhs-at-environment.sunderland.ac.uk

----------

Hi:

I am trying to figure out how to let lab users share diamond knives
provided by me. If you have an opinion on the shared use of diamond knives,
I would be interested in it.

Here is the setup: I oversee a small EM lab, most of our TEM users are
undergraduate and graduate students. The glory of molecular biology has
turned the heads of most of our faculty so the level of support for student
TEM is fairly low. However, we do get students and other users who want to
try a project that requires sectioning. As long as there is minimal cost,
many students get started and try sectioning using glass knives.
Eventually, they run into the limitations of glass knives and want to use a
diamond knife.

When I suggest that they purchase a diamond knife, the wind goes out of
their sails. Most say they cannot afford even to have an old knife from
their lab resharpened, let alone afford to buy a new knife. I would like
to help them but feel burdened by the problem of how to distribute diamond
knife privileges among semi-beginning users.

To add to the current problem, I have several old diamond knives that need
resharpening. Letting users have access to these knives in their current
condition is not much help. It will cost us several thousand dollars to get
them resharpened. I think we have some money in the lab budget that could
be used for sharpening. Of course once they are sharpened, do I want to
pass them out to multiple users?

So it boils down to something like this:

I have users who need access to a diamond knife to do their work. They say
they are too poor to afford a knife. I have knives and money to sharpen
them. Do I play the Sugar Daddy and sharpen the knives and just let them
use them? Do you have any sage advice about establishing a policy to help
these paupers get their work done?

I struggle with the questions of how to make users borrowing an expensive
knife responsible so they are careful and can contribute to replacing the
knife if they damage it. I wonder about the need to set up some kind of
charge to use the knife when for most practical purposes any single user
will (hopefully) leave it as good as new. I wonder if it would be OK to let
more than one person use a knife at the same time. It goes on and on.

If you have worked on this problem or just have some personal insights to
help me I would appreciate hearing from you.




Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
FAX (408) 429-0146
jmkrupp-at-cats.ucsc.edu



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From: MicroToday-at-aol.com
Date: Thu, 5 Sep 1996 09:10:55 -0400
Subject: FEI/Philips Electrron Optics

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HOT OFF THE PRESS:

"Phililps Electron Optics and FEI Company to combine global operations:
Philips Electron Optics BV (Eindlven, The Netherlands) and FEI Company
(Hillsb oro, Oregon, USA) have today signed a letter of intent to enter into
an agreemnt to integrate their global operations. To accomplish this, FEI
will acquire substantially al of the assets of the Philips Electron Optics
business. In exchange, Philips will acquire approximnately 55% of the common
stock of FEI. The balance of the FEI common stock will be held by the
present FEI shareholders. FEI will continue to be publicly traded on Nasdaq.
Closing is currently expected at end 1996."

For full description of the arrangement, see the September issue of
Microscopy Today.
Don Grimes
Microscopy Today




From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Thu, 5 Sep 1996 07:30:54 -0700 (PDT)
Subject: Re: UNICRYL

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Hello Sara,

In response to your question about why we are going to Unicryl if it has
some problems to work out?

The main reason is to find a simple method that increases our
immunolabelling potential and retains good ultrastructure. Wether unicyl
is the answer I'm not so sure yet. But, at this point the alternative is
freeze substitution to increase our antigen yield. I'm hoping for a
simpler way.

By the way do know a Ms Lara Muffley? We hired her in our lab here in
Seattle. She is wonderfull !!!!!

Bob
Morphology Core
206-543-1088






From: John Best :      jbest-at-Sparc5.Microscopy.Com
Date: Thu, 05 Sep 1996 12:03:42 -0700
Subject: Hissing Detector.

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Message-ID: {322F240E.6D96-at-vicon.net}

Hello All,

The "hissing" detector is somewhat less mysterious now. It seems I have
a vacuum leak! The hissing was the LN2 bubbling. I was able to see this
by lowering a small flashlight into the dewar, just above the surface of
the LN2.

Apparently (comments please) an increase in the activity of the bubbling
after venting the chamber is an indicator of the integrity of the cold
finger, BE window, seals, etc.

Many thanks to Claudio Tarquinio of EVEX.

Also a special thank you to Harry Crossman who was kind enough to give me
a call and some very valuable advice.


Regards All,
John.

--
John W. Best ELMDAS Co. Email: jbest-at-vicon.net
P.O. Box 355, Alexandria, PA, USA 16611
Voice: 814-669-4474
WWW: http://www.vicon.net/~jbest





From: _ _ _ _ _ MARK W. LUND _ _ _ _ _ :      lundm-at-physc2.byu.edu
Date: Thu, 05 Sep 1996 11:10:36 MST/MDT
Subject: Hissing EDS detector

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Sender: lundm-at-physc1.byu.edu

John Best wrote (in part).

`We have a different problem today. I've recently installed an EDS
`detector on my SEM. After filling with LN2, allowing 24 hrs to cool,
`then topping off, I powered up the analyzer. There is a symptom of a
`problem, i.e. the count rate is high even with no xray source.
`
`It's a pulsed optical type preamp, and a check of the test point on the
`preamp revealed a +/- 1V sawtooth approx 10 mS in duration. The
`documentation indicates that with no xrays present this ramp should
`ascend very slowly.
`
`While looking for the cause, I noticed that the detector is "hissing".
`It's been 36 hrs from the LN2 top off, and the detector has used about
`20% of the LN2. I don't think this a necessarily high usage rate, but
`when listening near the top of the dewar, I can still hear something
`hapenning in there!
`
`What do you think? Contamination in the dewar? Ice? I'm thinking of
`pouring out the LN2, blowing the detector out with Argon and refilling
`it. Any suggestions? Is there a cleaning procedure?

John,
A short ramp without any x-ray flux usually means leakage current in
the detector. This could be caused by a warm detector, which could
happen because 1) the dewar has a poor vacuum, 2) the detector assembly
has a thermal short to the snout, or 3) the strap connecting the
cold finger to the LN2 tank has come loose. All of these problems
would cause the window, snout, or other external surfaces to get
cold--frost will form, etc.

Other, less likely, causes are contamination on the detector, damage
to the detector-fet assembly, etc. Some old detectors had an extra
LED that illuminated the detector in order to get the detector leakage
current higher than the JFET. There is a trim pot to adjust this,
which may have been tampered with.

Lastly, if the feedback connection between the external part of the
preamp and the dewar is not connected the whole thing will freak out,
but you probably wouldn't be getting a good (but fast) ramp.
best regards
mark




From: Warren Straszheim :      wesaia-at-iastate.edu
Date: Thu, 05 Sep 1996 13:45:09 -0500
Subject: Re: Hissing EDS detector

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Message-Id: {199609051846.NAA12301-at-mailhub.iastate.edu}
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I know mechanical vibration (tapping the side of the dewar) can generate
counts. Also, the chamber light can also flood the preamp with signal. But
the hissing sound... I have heard tell of something like that when the
window goes, but probably worse.

We had a few panes of the many on our window fail. When there was atmosphere
behind that window (i.e., after venting the sample chamber), we had a flood
of counts. After it pumped down (several minutes if not hours), we could
operate. As long as we used the airlock, we were okay.

Careful examination of the window revealed the failed panes the size of
pinholes. We sent the detector in for a replacement window. It might be
worth a check.

feAt 08:52 AM 9/5/96 -0700, you wrote:
} Hello All,
}
} First let me thank all of those who replied to my question about
} interfacing MAC's to PC's.
}
} We have a different problem today. I've recently installed an EDS
} detector on my SEM. After filling with LN2, allowing 24 hrs to cool,
} then topping off, I powered up the analyzer. There is a symptom of a
} problem, i.e. the count rate is high even with no xray source.
}
} It's a pulsed optical type preamp, and a check of the test point on the
} preamp revealed a +/- 1V sawtooth approx 10 mS in duration. The
} documentation indicates that with no xrays present this ramp should
} ascend very slowly.
}
} While looking for the cause, I noticed that the detector is "hissing".
} It's been 36 hrs from the LN2 top off, and the detector has used about
} 20% of the LN2. I don't think this a necessarily high usage rate, but
} when listening near the top of the dewar, I can still hear something
} hapenning in there!
}
} What do you think? Contamination in the dewar? Ice? I'm thinking of
} pouring out the LN2, blowing the detector out with Argon and refilling
} it. Any suggestions? Is there a cleaning procedure?
}
} Thank You most kindly in advance for any and all consideration of this
} matter.
}
} Regards,
} John.
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: FRIEDA CHRISTIE :      f.christie-at-rbge.org.uk
Date: Fri, 6 Sep 1996 12:18:20 BST
Subject: Mercury lamp blow-out

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Suggestions please on how best to deal with a mercury lamp blow-out.

Thanks in advance,

Frieda Christie
Royal Botanic Garden, Edinburgh




From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 6 Sep 1996 09:24:04 -0400 (EDT)
Subject: Re: Mercury lamp blow-out

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}
} Suggestions please on how best to deal with a mercury lamp blow-out.
}
Dear Frieda,
When I was using a 1 kW high-pressure Hg lamp, one blew out
just as I reached out to adjust the air flow--it made quite an im-
pression. When I called the company about repair of the housing,
they told me that this was the usual method of failure. The solu-
tion I used was to turn on the lamp and leave it on for the duration
of the experiment (and to run the experiment 24 hours a day). Since
I was doing a series of photochemistry experiments at different ex-
posures and wavelengths, this was feasible, although I had to come
into the lab at odd hours. Having the lamp on continuously extended
its lifetime by a large factor. After the experiment was done and
the lamp was shut off, it was discarded--before it blew. This may
not be a practical solution for you, but the key is not to subject
the lamp to more thermal stress (by turning it on and off) than ab-
solutely necessary, and to discard it before it explodes. Good luck.
Yours,
Bill Tivol




From: smita purushottam manusmare :      smanusma-at-students.uiuc.edu
Date: Fri, 6 Sep 1996 10:24:35 -0500 (CDT)
Subject: Unsubscribe

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From: James Martin :      James.S.Martin-at-williams.edu
Date: Fri, 6 Sep 1996 11:19:14 -0400 (EDT)
Subject: Re: Mercury lamp blow-out

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This thread may be of general interest. Could respondents please post to
the list? Thanks.

James Martin

On Fri, 6 Sep 1996, FRIEDA CHRISTIE wrote:

} Suggestions please on how best to deal with a mercury lamp blow-out.
}
} Thanks in advance,
}
} Frieda Christie
} Royal Botanic Garden, Edinburgh
}




From: Crossman, Harold :      crossman-at-rd.sylvania.com
Date: Fri, 6 Sep 1996 13:52:00 -0400
Subject: Cleaning standard

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Does anybody have, or know where I can obtain the following military
standard?

MIL-STD-1246C Product Cleanliness and Contamination Control Program


Thanks in advance,



Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.osram.de
www.siemens.com




From: rnessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Fri, 6 Sep 1996 14:16:14 -0500 (CDT)
Subject: Re: Mercury lamp blow-out

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On Fri, 6 Sep 1996, FRIEDA CHRISTIE wrote:

} Suggestions please on how best to deal with a mercury lamp blow-out.
}
} Thanks in advance,
}
} Frieda Christie
} Royal Botanic Garden, Edinburgh
We had one explode about four months ago. The user of the instrument
was very concerned about the exposure to mercury vapor. Since we are in a
university setting, I called our Health Protection Office for advice. They said
to consult the manufacturers data that accompanies replacement bulbs. The data
sheet says to clear the area, and allow the ventilation system to exchange the
air in the room. HPO didn't think that there would be enough liquid mercury to warrant
trying to wipe down the area. Consult the manufacturer of the lamp in question.


Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu





From: Matt Schibler :      schibler-at-ljcrf.edu
Date: Fri, 6 Sep 1996 15:03:34 -0700
Subject: Mercury burner blowout

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At 12:18 PM 9/6/96 BST, you wrote:
} Suggestions please on how best to deal with a mercury lamp blow-out.
}
} Thanks in advance,
}
} Frieda Christie
} Royal Botanic Garden, Edinburgh
}
} Frieda,

I have had this happen two or three times. Once it was due to using past
the rated life of the burner and I had two 200W lamps explode due to a
faulty power supply.

WHAT TO DO:

1) Close off the room immediately. This is mercury vapor (a small amount,
but mercury vapor is highly toxic (it has been known to kill careless gold
amalgamators). You want the mercury to return to liquid form. It is going
to be in a few places.

2) call your institutional safety officers and find out what they do for a
mercury cleanup. This is not a great amount of mercury, but they may want
to deal with it according to your local environmental laws.

3) After that, you're going to have to check the condition of your lamp
housing. If you have a lamp housing with a parabolic mirror, it may have
been shattered by the explosion and you will have to send it back to the
manufacturer for repair.

4) Determine the cause of the blowup. Posible ones are:

a) use beyond rated life. This is the most common cause of lampo blowout.
The glass inside the burners gradually gets coated inside with a gray or
black film, even with proper use. This will lead to the lamp burning hotter
as it nears the end of its rated life. Eventually, booom!
As I understand it rated lives are as follows:

50W HBO 100 hrs
100W HBO 200 hrs
200w HBO 400 hrs
(Anyone please correct me if I am misinformed on this point).

b) improper alignment of the burner. If there is a parabolic mirror in your
lamp housing, it is important that you do not overlap the mirror image of
the arc with the real image. This can easily lead to oveheating of the high
pressure bulb. Refer to the manual for your lamp housing for arc alignment
instructions.

c) Faulty power supply. Occasionally, a power supply may overload the
burner. As I stated, I have had this happen a couple of times. Get the
power supply either replaced or repaired.

I hope this helps you and others on the list. Please add any other useful
commments.

Matt Schibler
Matthew J. Schibler, Ph.D.

Cell Imaging Facility
The Burnham Institute
(La Jolla Cancer Research Foundation)
10901 North Torrey Pines Road
La Jolla, CA 92037

P (619) 455-6480 x3206
F (619) 646-3197
E-mail: schibler-at-ljcrf.edu





From: cytoana-at-univ-lyon1.fr
Date: Tue, 13 Aug 1996 17:40:56 +0200
Subject: Mercury burner blowout

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unsubscribe mycroscopy cytoana-at-cismsun.univ-lyon1.fr





From: Dr. Molnar Peter :      molnarp-at-lib.dote.hu
Date: Sat, 7 Sep 1996 11:11:06 +100
Subject: NIH image

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Hi Fellow Microscopists:
I am trying to set up an image analysis operation and would like to
get acquinted with the NIH software. Please help me with the
following problems.
1. How do I access the software?
2. I have been warned that it is only usable on Macs. Is this true?
My PC is an IBM compatible, 100 MHz, 32 Mbyte RAM, 500 Mbyte
Winchester. Do I have any chance to run the NIH program on it?
All help would be highly appreciated.
Thanks in advance
Peter
Please reply directly to: molnarp-at-lib.dote.hu




From: Xianying Burany :      xianying-at-udel.edu
Date: Sat, 7 Sep 1996 13:32:44 -0400 (EDT)
Subject: Acknowledgement

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I thank all of those who replied to my questions about dimpler, furnace
and EDS detector windows.

Sandy







From: allardlfjr-at-ornl.gov (Larry Allard)
Date: Sat, 7 Sep 1996 22:16:39 -0400
Subject: Used SEMs

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Message-ID: {n1369996934.23937-at-qmgate.anl.gov}

Hi all:

Periodically we see posts of used SEMs for sale, but I haven't saved any of
them. I am gathering info for a colleague who is interested in purchasing
a reliable used SEM including an EDS detector, and can spend up to 50K.
Please e-mail me directly if you have a candidate, or know of someone who
does.

Thanks in advance.

Larry






From: Edward J. Huff :      huffe-at-potassium.chem.nyu.edu
Date: Sat, 7 Sep 1996 23:00:20 -0400
Subject: Re: NIH image

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Look on
{li} {a href="http://www.cs.ubc.ca/spider/ladic/executor.html"} Using NIH Image on a PC {/a}
{li} {a href="http://rsb.info.nih.gov/nih-image/"} NIH-Image home page {/a} and {a href="http://rsb.info.nih.gov/nih-image/manual/contents.html"} online manual {/a}
{li} {a href="http://corn.eng.buffalo.edu/www/ConfocalList/readme.html"} Confocal Mailing List {/a}




From: Krueger, Eugene :      krueger.eugene-at-mayo.edu
Date: 9 Sep 1996 10:23:34 -0600
Subject: Pinkel filter set

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I'm just wondering what people's opinions are of the Pinkel polychroic
filter set. Is it easy to use? Is it really any better than using a slider
cube? My lab has need of several new filters, and it appears that the Pinkel
set may fill our needs. Any observations, suggestions, etc. are welcome.

TIA

Eugene Krueger
GI Research
Mayo Foundation
krueger.eugene-at-mayo.edu




From: Dave Teter :      teter-at-lanl.gov
Date: Mon, 09 Sep 1996 08:48:38 -0600
Subject: Re: NIH image

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Message-Id: {2.2.32.19960909144838.00693c5c-at-mst.lanl.gov}
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Look on
{li} {a href="http://rsb.info.nih.gov/nih-image/"} NIH-Image home page {/a}

There is a program called ImagePC which is based on NIH Image but rewritten
for 32bit WIN95. I haven't tried it yet, but it looks to have all the same
functions as the MAC version. Give it a whirl.
*******************************************************
David F. Teter
Los Alamos National Laboratory
Materials Science and Technology: Metallurgy (MST-6)
Mail Stop: G755
Los Alamos, NM 87545
ph: (505)665-9975 fax: (505) 667-8021
e-mail: teter-at-lanl.gov
*******************************************************





From: sling-at-erenj.com (Shiun Ling)
Date: Mon, 09 Sep 1996 12:02:04 -0400
Subject: Postdoc Position at Exxon Research

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Postdoctoral Research Fellow Position in
Transmission Electron Microscopy for Corrosion Chemistry
Corporate Research Laboratories, Exxon Research & Engineering Company

The Corporate Research Laboratories of Exxon Research and Engineering
Company has a Postdoctoral opening in the area of characterizing films
formed on steel and alloy surfaces. Specifically, we are seeking candidates
having Ph.D. degree in Materials Science or a related discipline, and having
expertise in the use of TEM techniques to investigate the microstructure and
microchemistry of inorganic surface films. Capability in diffraction and
high resolution imaging techniques will be an added asset. The selected
candidate will work in the Corrosion Chemistry Group at the Laboratories.

Exxon's Corporate Research Laboratories are located in scenic, rural
western New Jersey, about an hour west of New York City and 45 minutes
northwest of Princeton. The laboratories perform basic and applied research
in support of Exxon Corporation's worldwide scientific, technological, and
business needs.

Applicants for this position should send a resume, a publication list, a
summary of major research interests, and three letters of reference to:

Dr. Shiun LING
Exxon Research & Engineering Company
Corporate Research Laboratories
Room LA 388
Route 22 East, Clinton Township
Annandale, NJ 08801-0998
FAX: (908) 305-3355
E-mail: sling-at-erenj.com

Equal Opportunity Employer M/F/H/V

-----------------------------------------------------------------------
Best Regards,
Shiun LING
LA 388, Exxon Research & Engineering Co., Annandale, NJ 08801
Tel: (908) 730-2337; FAX: (908) 730-3355





From: gss2-at-psu.edu (Gary Settles)
Date: Mon, 09 Sep 1996 14:18:33 -0400
Subject: LM - Julius Rheinberg

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Dear colleagues:

Everyone knows about Rheinberg illumination, which was supposedly eclipsed
in the 1950's by Zernike's Nobel-prize-winning phase-contrast approach but keeps
cropping up, perhaps becuase of the beautiful results it produces. I'm
doing some
historical research on Julius Rheinberg for a book I'm writing on schlieren
and shadowgraph techniques, which features a chapter on the close connection
between these techniques and microscopy. If anyone has ever seen a photo of
Royal Microscopical Society Fellow Julius Rheinberg or any biographical
information on him, I'd sure appreciate hearing about it.

Thanks!


------------------------------------------------------------------
Gary S. Settles
Professor of Mechanical Engineering
Director, Gas Dynamics Lab phone: (814) 863-1504
Penn State University fax: (814) 865-0118
301D Reber Bldg. email: gss2-at-psu.edu
University Park, PA 16802 USA
http://www.me.psu.edu/psgdl/index.html
------------------------------------------------------------------





From: Steven D. Majewski :      sdm7g-at-Virginia.EDU
Date: Mon, 9 Sep 1996 17:52:17 -0400 (EDT)
Subject: DTSA <=> EMMFF file conversion

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Does anyone have a utility to/from convert NIST DTSA spectral
binary data files to EMSA/MAS Standard File Format ?

---| Steven D. Majewski (804-982-0831) {sdm7g-at-Virginia.EDU} |---
---| Computer Systems Engineer University of Virginia |---
---| Department of Molecular Physiology and Biological Physics |---
---| Box 449 Health Science Center Charlottesville,VA 22908 |---
[ "The grass is always greener, except at t=0" - Stan Kelly-Bootle ]





From: Roger Wallis :      rogergm-at-ozemail.com.au
Date: Tue, 10 Sep 1996 14:39:09 +-1000
Subject: Objective Lenses for Sale

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Message-ID: {01BB9F25.D5EE6F40-at-slmel9p41.ozemail.com.au}

Dear List,

A customer of ours has a selection of brand new, never used Olympus =
objectives he wishes to sell. They are all 160 tube length and suitable =
for the CH2, BH2, AH2, CK2 and IMT2 series of scopes and anything else =
that uses the 160 TL. The prices shown are today's recommended lists. =
Any offers for one or all of these will be seriously considered. If =
there is any interest, please contact me on the e-mail address below.=20

Objective List Price AUD$
D Apo 40X UV /RIO NA 1.3 oil WD 0.12 UV Corrected $1,440
D Apo 100X UV/RIO 1.3 oil 0.12 UV Corrected $1,570
D Plan Apo 100x UVPL/R 1.3 oil 0.16 UV Corrected, Phase cont. =
$2,770
LWDCDPL20X NA 0.4 cover slip corrected 0-2mm long working Plan $985
LWDCDPL40X NA 0.55 cover slip corrected 0-2mm long working Plan $1,755
SPL10X NA 0.3 WD 7.5 corrected for DIC $467
ED10X NA 0.25 WD 6.3 Good quality student achromat $97 x6

Eyepieces
GWH10X-CD Widefield with cross hair for SZ, SZH etc. $275
GWH10X-D Focussable match for above $199

Misc.
BH2-DMB "Blue" fluorescence cube for BH2 with BP490 filter $2,000

Thanks and regards,



Roger Wallis
General Manager
Optiscan P/L
Confocal Imaging
PO Box 1066
Mt. Waverley MDC
Victoria 3149 Australia
Tel: (61) 3-9562 7741
Fax: (61) 3-9562-7742
Mob:(61) 0412-004-252
e-mail: rogergm-at-ozemail.com.au
Web Site: http://www.optiscan.com.au
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D




From: pyk-at-ornl.gov (Stephen J. Pennycook)
Date: Tue, 10 Sep 1996 00:12:26 -0400
Subject: Re: Postdoc Positions in Materials Physics

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ruehle-at-hrem.mpi-stuttgart.mpg.de, j-gibson-at-uiuc.edu,
jsilcox-at-msc.cornell.edu, UDahmen-at-lbl.gov, spencej-at-csss2.la.asu.edu,
smithd-at-csss2.la.asu.edu, rez-at-csss2.la.asu.edu, cel1-at-lehigh.edu,
del-at-sol1.lrsm.upenn.edu, BABCOCK-at-coeadm.engr.wisc.edu,
andy.norman-at-materials.oxford.ac.uk, carpenter-at-csss2.la.asu.edu,
cbcarter-at-maroon.tc.umn.edu, eades-at-uimrl7.mrl.uiuc.edu,
D.Bird-at-bath.ac.uk, imajid-at-prism.mit.edu, egerton-at-phys.ualberta.ca,
goodhew-at-LIVERPOOL.AC.UK, rgronsky-at-garnet.berkeley.edu,
zaluzek-at-aaem.amc.anl.gov, dbw1-at-lehigh.edu, leapman-at-helix.nih.gov,
jrmicha-at-sandia.gov, tonygr-at-eagle.mit.edu, granstis-at-phys.uts.edu.au,
kruit-at-dutndo7.tn.tudelft.nl, jr43-at-phy.cam.ac.uk, wos1-at-cus.cam.ac.uk,
van_dyck-at-ruca.ua.ac.be, batson-at-watson.ibm.com, clarke-at-eci1.ucsb.edu,
temitchell-at-lanl.gov, alexanderkb-at-ornl.gov, ALLARDLFJR-at-ornl.gov,
vog-at-ornl.gov

Dear colleagues,

I would be most grateful if you would bring the position below to the attention
of potential candidates.

Many thanks,

Steve Pennycook


JOINT POSTDOCTORAL POSITION IN ELECTRON MICROSCOPY OF SUPERCONDUCTORS

OAK RIDGE NATIONAL LABORATORY/UNIVERSITY OF ILLINOIS, CHICAGO

An outstanding candidate is sought for a program on correlating
superconducting transport properties to grain boundary atomic structure and
chemistry by combined Z-contrast microscopy and EELS, using thin films
grown on bicrystal substrates and also wire samples. This is a joint
program between ORNL (S. J. Pennycook) and UIC (N. D. Browning), using the
VG
Microscopes HB603 300 kV scanning transmission electron microscope with a
1.26A probe size, and the HB501UX 100 kV microscope with a
high sensitivity parallel EELS capability, both located at ORNL. Based on
recent success in using the structural unit model to explain the
exponential decrease in critical current with increasing grain boundary
misorientation, we anticipate this program will make substantial progress
in understanding the link between grain boundary atomic structure and the
macroscopic
transport properties of thin films and wires.


Successful candidates will be recent Ph.D. graduates in physics,
metallurgy, or materials science with a sound background in the relevent
materials issues and a burning ambition to develop a forefront area in
materials physics. If this is you, send your resume and publication list
to Dr. S. J. Pennycook at the address below. Prior experience using
transmission electron microscopy is essential. Positions are for one year
initially, normally
renewed for a second year and possibly a third. ORNL is a multipurpose
national laboratory managed by Lockheed Martin Energy Research Corporation
for the U.S. Department of Energy. ORNL is an equal opportunity employer
committed to building and maintaining a diverse work force.



----------------------------------------------------------------------------
-----------------------------------------Stephen J. Pennycook
Corporate Fellow and Electron Microscopy Group Leader
Oak Ridge National Laboratory
Solid State Division
PO Box 2008
Oak Ridge TN 37831-6030

phone: (423) 574-5504
fax: (423) 574-4143
----------------------------------------------------------------------------
------------------------------------------






From: SCM!ATitkov-at-scmaust.attmail.com (SCM!ATitkov)
Date: Tue, 10 Sep 1996 15:46:42 +0800
Subject: OSIRIS image processing software

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To: Microscopy-at-Sparc5.Microscopy.Com (Microscopy Listserver U.S.A.)

Dear Microscopists,

I am looking for OSIRIS, image processing freeware, which enables to
input calibration and manually measure features using a mouse. As far as
I remember it was designed for medical applications.

I would appreciate your help in locating it. Also, if you know other
free/shareware programs running on PC (DOS or Windows 3.1) with the same
capability, could you please let me know.

Alexander Titkov

SCM Chemicals Ltd.
PO Box 245
Bunbury WA 6231
Australia
Ph (097) 808 505






From: ROBIN CROSS :      EURC-at-giraffe.ru.ac.za
Date: Tue, 10 Sep 1996 11:04:55 GMT+0200
Subject: fats in leather

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A student would like to be able to measure the extent
of penetration of fats into leather. Does anyone know of LM
stains which will show this?

Thanks


Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)




From: Helen McCall - SAHFOS :      hmc-at-unixb.nerc-pml.ac.uk
Date: Tue, 10 Sep 1996 11:17:18 +0100
Subject: Re: LM - Julius Rheinberg

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Dear colleagues;

Prof. Gary Settles writes:

} Everyone knows about Rheinberg illumination, which was supposedly eclipsed
} in the 1950's by Zernike's Nobel-prize-winning phase-contrast approach but
} keeps cropping up, perhaps becuase of the beautiful results it produces.

Well I am not everyone because I do not know about Rheinberg illumination.

Could anyone explain it to me because I am interested in any light microscopy
techniques which might help me to identify Dinoflagellate cysts?

Thanks.

Helen McCall (PhD Student)
Sir Alister Hardy Foundation for Ocean Science
The Laboratory,
Citadel Hill,
Plymouth PL1 2PB
Great Britain

E-Mail: H.McCall-at-pml.ac.uk




From: Dietmar.Reiter-at-uibk.ac.at (dietmar.reiter-at-uibk.ac.at)
Date: Tue, 10 Sep 1996 13:17:39 +0100
Subject: Re:OSIRIS image processing software

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X-Sender: c71956-at-dm.uibk.ac.at
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Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii

At 15:46 Uhr on 10.9.1996, SCM!ATitkov wrote:

} Dear Microscopists,
}
} I am looking for OSIRIS, image processing freeware, which enables to
} input calibration and manually measure features using a mouse. As far as
} I remember it was designed for medical applications.

http://expasy.hcuge.ch/www/UIN/osiris.html

} I would appreciate your help in locating it. Also, if you know other
} free/shareware programs running on PC (DOS or Windows 3.1) with the same
} capability, could you please let me know.

Try to find something at:
http://www.cs.ubc.ca/spider/ladic/softibm.html
http://www.ee.princeton.edu/~icip95/iplink/index.html

regards, -Dietmar-

*** Dietmar Reiter {dietmar.reiter-at-uibk.ac.at} ****************
*** Dept. of Zoology, Univ. of Innsbruck, Technikerstrasse 25 ***
*** A - 6020 Innsbruck, Austria *** fax: (+43)512-507-2930 ***






From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Tue, 10 Sep 1996 12:50:10 +0100 (BST)
Subject: Re: Image conversion

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On Tue, 10 Sep 96 15:40:35 GMT
makroczy-at-ccsun.tuke.sk wrote:
} Does anybody know about image conversion utility
(sharware, freeware or
} commercial product) to convert images from/to Link
AN10000 EDX system (*.IM)
} and IBM PC computer (e.g. *.pcx, *.tiff, *.jpg...)? Thank
you very much.
}
}
} Peter Makroczy
} Technical University of Kosice
} Dept. of Materials Science
} Park Komenskeho 11
} 040 01 Kosice
} Slovak Republic
} E-mail: makroczy-at-ccsun.tuke.sk
}
I had the same problem. For some reason whenever I
asked the Link people, they denied all knowledge of a
solution. There is however, a solution. Provided you have
a reasonably late version of the Link operating system (Rev
5.22 for the setup and rev 5.25 utilities), you can convert
the image to a PC format version, still .IM. PC Image from
Foster Finlay Associates, Newcastle Technopole, Kings
Manor, Newcastle upon Tyne NE1 6PA england, can read
.IM files and convert them to a number of formats. It is
commercial software. I have found it to work reasonably
well, although the instruction manual is not particularly
helpful.

Good luck with your quest,

Dr Eric Lachowski
University of Aberdeen
Department of Chemistry
tel +44 1224 272934
fax +44 1224 272921





From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Tue, 10 Sep 1996 12:53:41 +0100 (BST)
Subject: Re: Image conversion

Contents Retrieved from Microscopy Listserver Archives
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On Tue, 10 Sep 96 15:40:35 GMT
makroczy-at-ccsun.tuke.sk wrote:
} Does anybody know about image conversion utility
(sharware, freeware or
} commercial product) to convert images from/to Link
AN10000 EDX system (*.IM)
} and IBM PC computer (e.g. *.pcx, *.tiff, *.jpg...)? Thank
you very much.
}
}
} Peter Makroczy
} Technical University of Kosice
} Dept. of Materials Science
} Park Komenskeho 11
} 040 01 Kosice
} Slovak Republic
} E-mail: makroczy-at-ccsun.tuke.sk
}
I had the same problem. For some reason whenever I
asked the Link people, they denied all knowledge of a
solution. There is however, a solution. Provided you have
a reasonably late version of the Link operating system (Rev
5.22 for the setup and rev 5.25 utilities), you can convert
the image to a PC format version, still .IM. PC Image from
Foster Finlay Associates, Newcastle Technopole, Kings
Manor, Newcastle upon Tyne NE1 6PA england, can read
.IM files and convert them to a number of formats. It is
commercial software. I have found it to work reasonably
well, although the instruction manual is not particularly
helpful.

Good luck with your quest,

Dr Eric Lachowski
University of Aberdeen
Department of Chemistry
Old Aberdeen, Scotland
tel +44 1224 272934
fax +44 1224 272921





From: Dave.Strecker-at-po.cle.ab.com (Dave Strecker)
Date: 9/10/96 3:46 PM
Subject: OSIRIS image processing software

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Mime-Version: 1.0
SCM!ATitkov-at-scmaust.attmail.com (SCM!ATitkov)
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
Content-Description: cc:Mail note part

Alexander,

OSIRIS has a web information page at
'http://expasy.hcuge.ch/www/UIN/UIN.html' or you can just get the
software by ftp by to expasy.hcuge.ch using username "anonymous" and
password of your e-mail address. Good luck.

Dave Strecker



______________________________ Reply Separator _________________________________


Dear Microscopists,

I am looking for OSIRIS, image processing freeware, which enables to
input calibration and manually measure features using a mouse. As far as
I remember it was designed for medical applications.

I would appreciate your help in locating it. Also, if you know other
free/shareware programs running on PC (DOS or Windows 3.1) with the same
capability, could you please let me know.

Alexander Titkov

SCM Chemicals Ltd.
PO Box 245
Bunbury WA 6231
Australia
Ph (097) 808 505






From: csedax-at-alpha.arcride.edu.ar
Date: Tue, 10 Sep 1996 13:02:07 -2036
Subject: SEM: again about tensile and torsion testing

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Hi everyone,

ten days ago I've sent a message to all of you asking for
references about mechanical properties, tensile and torsion test by SEM. Since
I've got just two messages about the subject, I try again in case someone else
can help me.


Thanks again. The old message follows......

Daniel F. Imbert

...................

I am a student from Argentina, and I am interested in the study of materials and the
next items: their mechanical properties, tensile and torsion test in diferents kinds of
materials like metals, polymers, ceramics, etc.. All this items refer to use of the
scanning electron microscopy.

If any of you know references about this subjet or names of people working on this
please send me a e-mail message if possible. I appreciate very much your attention,
thanks a lot.


Daniel F. Imbert

Centro Regional de Investigacion
y Desarrollo de Santa Fe.
Guemes 3450 - 3000 Santa Fe.
Argentina.

csedax-at-arcride.edu.ar




From: Marc C. Brande, MS, Founder :      mcbrande-at-sierra.net
Date: Tue, 10 Sep 1996 10:40:58 +0000
Subject: Free Brochure: Cell Bio/Imaging Services at Your Site

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Message-Id: {323545BA.5250-at-sierra.net}

Email your Full Name/Address/FAX to: mcbrande-at-sierra.net




From: hudakjm-at-mcmaster.ca (John Hudak)
Date: Tue, 10 Sep 1996 13:47:29 -0400 (EDT)
Subject: Re: Image conversion

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On our QX-2000 system (which is later than the AN10000) there is a small
program called DEMON/TIFF Convert. This will convert the .IM file to a .TI
file (only two character extensions allowed by Link) and dump it to a 720K
DOS formatted disk. It's a bit fussy to use and the conversion takes about
5 min. per 512x512 file. You'll have to rename the file for the .TIF
extension. The image comes out as a negative so I have invert it in a
viewer program. The other problem I found was that many viewers would not
read this TIFF file for some reason. I can only view them with Paint Shop
Pro. Once I've got it in there I can change it to a positive image and
resave it as whatever format I like. I don't know if the conversion program
will work with an AN10000 but it might be worth a try to see if Link can
supply it to you.

A company in Finland called Picomega, Ltd. was also offering a program
called 'Linker' to read .IM files. I'm afraid I don't have the address.
The fellow at the company is Esa Wainio.

--------------------------------------------------------
John Hudak hudakjm-at-mcmaster.ca
Electron Optics
Brockhouse Institute for Materials Research
McMaster University
Hamilton, Ontario, Canada








From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Tue, 10 Sep 1996 07:18:42 -0700 (PDT)
Subject: Re: LM - Julius Rheinberg

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On Tue, 10 Sep 1996, Helen McCall - SAHFOS wrote:

} Dear colleagues;
}
} Prof. Gary Settles writes:
}
} } Everyone knows about Rheinberg illumination, which was supposedly eclipsed
} } in the 1950's by Zernike's Nobel-prize-winning phase-contrast approach but
} } keeps cropping up, perhaps becuase of the beautiful results it produces.
}
} Well I am not everyone because I do not know about Rheinberg illumination.
}
} Could anyone explain it to me because I am interested in any light microscopy
} techniques which might help me to identify Dinoflagellate cysts?
}
} Thanks.
}
} Helen McCall (PhD Student)
} Sir Alister Hardy Foundation for Ocean Science
} The Laboratory,
} Citadel Hill,
} Plymouth PL1 2PB
} Great Britain
}
} E-Mail: H.McCall-at-pml.ac.uk
}
Hi Helen,

It is a technique similar to darkfield, but you replace the darkfield
stop with a color filter and the outside ring (,that would normally be the
only illuminating light that bounces off the specimen),to another colored
filter. Opposite colors work nice. So the backround of your field is the
color of the inner filter and any specimen capable of diffracting light
will be the color of your outer filter. We use to make these filters out
of kodak gelatin filters. Also you must make the inner disk much darker
than the outer, using nuetral density and you must match the inner disk
size to the NA of your objective just like darkfield. It can give trully
beautiful results.

Bob
Morphology Core
U of W
Seattle WA





From: dbw1-at-lehigh.edu (David B. Williams)
Date: Tue, 10 Sep 1996 08:45:30 -0500
Subject: David Smith

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X-Sender: dbw1-at-mail.Lehigh.EDU
Message-Id: {v01510125ae5b2142701c-at-[128.180.55.51]}
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ruehle-at-hrem.mpi-stuttgart.mpg.de, j-gibson-at-uiuc.edu,
jsilcox-at-msc.cornell.edu, UDahmen-at-lbl.gov, spencej-at-csss2.la.asu.edu,
smithd-at-csss2.la.asu.edu, rez-at-csss2.la.asu.edu, cel1-at-lehigh.edu,
del-at-sol1.lrsm.upenn.edu, BABCOCK-at-coeadm.engr.wisc.edu,
andy.norman-at-materials.oxford.ac.uk, carpenter-at-csss2.la.asu.edu,
cbcarter-at-maroon.tc.umn.edu, eades-at-uimrl7.mrl.uiuc.edu,
D.Bird-at-bath.ac.uk, imajid-at-prism.mit.edu, egerton-at-phys.ualberta.ca,
goodhew-at-LIVERPOOL.AC.UK, rgronsky-at-garnet.berkeley.edu,
zaluzek-at-aaem.amc.anl.gov, dbw1-at-lehigh.edu, leapman-at-helix.nih.gov,
jrmicha-at-sandia.gov, tonygr-at-eagle.mit.edu, granstis-at-phys.uts.edu.au,
kruit-at-dutndo7.tn.tudelft.nl, jr43-at-phy.cam.ac.uk, wos1-at-cus.cam.ac.uk,
van_dyck-at-ruca.ua.ac.be, batson-at-watson.ibm.com, clarke-at-eci1.ucsb.edu,
temitchell-at-lanl.gov, alexanderkb-at-ornl.gov, ALLARDLFJR-at-ornl.gov,
vog-at-ornl.gov

Dear Colleagues:

I regret to inform you that Professor David A. Smith died suddenly of heart
attack early on Sunday morning. He was fifty two. He is survived by his
wife Carol Smith-Nichols, a daughter Anna, eighteen months, a son Griffin,
to be born in November, and Howard, Patrick and Rosalind in Somers, New
York.

The will be a funeral service at Lehigh University's Chapel on Thursday
Septemeber 12 at 4:10 pm.

Donations to the Anna and Griffin Smith Fund are being accepted at the
Lafayette Bank Rt. 512 and Crawford Drive, Bethlehem PA 18017.

If you need any further information, please contact me directly. I can fax
a map if you wish to attend the funeral.

Dave Williams

PS. Please accept my apologies if you receive this message via several
email listings.









From: Warren Straszheim :      wes-at-ameslab.gov
Date: Tue, 10 Sep 1996 08:43:22 -0500
Subject: Re: Image conversion

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There is an option to export to TIFF and BMP in our Link Isis software.
Isn't there such an option in yours? The difficulty is in getting the job
done. It represents a few keystrokes for each file, and we have lots of files.

Therefore, we are in the process of writing a program to convert Link Isis
*.IM files to TIFF format in bulk. That is, we would like to do it outside
of the Link program structure and be able to select multiple files and to
automate the process. I don't know how long it will take. I am guiding a
student in the project helping him with what I know of TIFF and decoding the
IM format as we can. I think we will be able and glad to share the result
when we are finished.

I would suppose the AN10000 files would be the same. Perhaps you can send me
one with whatever description you have of it, e.g., 512x400, 8 bit image
file. You may wish to send both an image and an x-ray map set (at least a
couple of elements). We would be glad to play with it.

Of course, if this has already been done, I would be glad to find out too.

At 03:40 PM 9/10/96 +0000, you wrote:
} Does anybody know about image conversion utility (sharware, freeware or
} commercial product) to convert images from/to Link AN10000 EDX system (*.IM)
} and IBM PC computer (e.g. *.pcx, *.tiff, *.jpg...)? Thank you very much.
}
}
} Peter Makroczy
} Technical University of Kosice
} Dept. of Materials Science
} Park Komenskeho 11
} 040 01 Kosice
} Slovak Republic
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Eric Steel :      steel-at-enh.nist.gov
Date: Tue, 10 Sep 1996 16:10:58 -0400 (EDT)
Subject: Re: DTSA <=> EMMFF file conversion

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}
} Does anyone have a utility to/from convert NIST DTSA spectral
} binary data files to EMSA/MAS Standard File Format ?
}
} ---| Steven D. Majewski (804-982-0831) {sdm7g-at-Virginia.EDU} |---
} ---| Computer Systems Engineer University of Virginia |---
} ---| Department of Molecular Physiology and Biological Physics |---
} ---| Box 449 Health Science Center Charlottesville,VA 22908 |---
} [ "The grass is always greener, except at t=0" - Stan Kelly-Bootle ]
}

Version 2.5 of NIST Desktop Spectrum Analyzer (DTSA) includes a plug-in for
reading and writing of the MSA/MAS standard file format. This version
should be available from the NIST Standard Reference Data office
(301-975-2208) in about a month (its in the mail...)


Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov
Microanalysis Research Group Office: 301-975-3902
N.I.S.T. FAX: 301-216-1134
Bldg. 222/Rm A113
Gaithersburg MD 20899





From: Mount, Richard :      MOUNTR-at-hermes.is.sickkids.on.ca
Date: Tue, 10 Sep 1996 11:04:00 -0500
Subject: re: Rheinberg illumination

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Message-ID: {Megw.374257-at-hermes}


{Text_1}
Helen McCall asked about Rheinberg illumination. A good how
to guide can be found in Kodak's "Photography Through the
Microscope" book. The following description is taken from
the Molecular Expressions web page at:
http://micro.magnet.fsu.edu/micro/primer/setup.html

Another method of contrast enhancement was developed by
microscopist Julius Rheinberg in 1896. Rheinberg
illumination is often referred to as optical
staining, and lends an exciting spectrum of color and
highlights to conventional microscopy techniques. It is
especially useful for amorphous and unstained
samples which lack sufficient detail for successful
photomicrography. Rheinberg illumination is similar to
conventional darkfield illumination except that the
central opaque light stop at the base of the substage
condenser (Figure 3) is substituted for a colored central
stop. A sample viewed with the colored
central stop will appear white on a background the color of
the stop. Addition of a colored transparent annular filter
will add color to the sample. A
variety of materials can be employed for constructing the
colored stops and annular rings. Colored cellulose acetate
or gelatin filters are probably the
most convenient, and many graphics supply houses sell, or
sometimes give away, small sample books of these materials.
By experimenting with a variety
of different colored stops and annular filters, a wide
spectrum of different images can be obtained.




From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: 9/10/96 11:04 AM
Subject: fats in leather

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Mime-Version: 1.0

Robin:

One stain that you might try is Alcian blue (Ingrain blue). It is very
specific to negative surface charges and carbonyl groups, and might do the
trick for you. I recently had success using it as a 0.05% aqueous solution
to identify glycerol monostearates. Staining time was only about 10
minutes at room temp.

Regards,

-Bob
***********************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA. 91711
USA
ph: (909)399-1311
email: Bob_Citron-at-cc.chiron.com
***********************************

______________________________ Reply Separator _________________________________


A student would like to be able to measure the extent
of penetration of fats into leather. Does anyone know of LM
stains which will show this?

Thanks


Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)




From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 10 Sep 1996 17:16:48 -1000 (HST)
Subject: EELS/ESI

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Message-Id: {9609110002.AA5251-at-czjnotes03}
To: Microscopy {Microscopy-at-Sparc5.Microscopy.Com} ,
confocal {confocal-at-ubvm.cc.buffalo.edu} , spm {spm-at-di.com}

Aloha, Microscopists!

What is the difference between EELS (electron energy loss spectroscopy)
and ESI (electron spectroscopic imaging)? Is ESI with deltaE } 0eV the
same as EELS? And when one speaks of "energy filtering" on TEM, is one
refering to ESI only, or is EELS included?

Confused in Honolulu,
Tina

www.pbrc.hawaii.edu/bemf/microangelo.html
***************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu *
***************************************************************************





From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Tue, 10 Sep 1996 23:09:48 -0500
Subject: Re: EELS/ESI

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Tina

EELS is spectroscopy of an area or point. You get a "spectrum" just
similiar to an X-ray spectrum when you select an area
using scanning or fixed spot analysis.

ELSI is an imaging technique where you form an image with
a fixed energy window, similiar to X-ray Mapping. You select the
energy window you want to image and form a "energy filtered"
image. If the window surrounds the zero loss peak then you
have a pseudo-elastic image. If the window is on a specific
energy loss then you have an inelastic image corresponding
to the energy loss you have selected. If you want to form an
image of a specific "element" in ELSI, you must do some
processing as the background (unlike XEDS) is highly non-linear
so you must have windows before, at and after each energy
loss edge. With suitable processing you get elemental and/or
chemical images.

There is also the Spectrum Imaging modality, which was
pioneered by Trebbia and Colliex Group in Orsay and has been
since developed a great deal by multiple groups. Here you record
a spectrum at each point of an image (if you are running in
STEM mode). Or multiple Energy Filtered Images in the EFTEM
mode. You can then interrogate the 3D data set and get the
best of both worlds (spectroscopy and/or imaging).

Look at the books by Egerton( EELS in the Electron Microscope)
Plenum Press, or Reimer (Transmission Electron Microscopy)
Springer Series. Or the article by Hunt and Leapman in JMSA
Volume 1 Issue #3
(http://www.msa.microscopy.com/JMSA/JMSA1995/Vol1-3.html)

Nestor
Your Friendly Neighborhood SysOp



} Aloha, Microscopists!
}
} What is the difference between EELS (electron energy loss spectroscopy)
} and ESI (electron spectroscopic imaging)? Is ESI with deltaE } 0eV the
} same as EELS? And when one speaks of "energy filtering" on TEM, is one
} refering to ESI only, or is EELS included?
}
} Confused in Honolulu,
} Tina
}
} www.pbrc.hawaii.edu/bemf/microangelo.html
} ***************************************************************************
} * Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
} * Biological Electron Microscope Facility * (808) 956-6251 *
} * University of Hawaii at Manoa * http://www.pbrc.hawaii.edu *
} ***************************************************************************







From: Stuart Kearns :      Stuart.Kearns-at-bristol.ac.uk
Date: Wed, 11 Sep 1996 10:45:03 +0100 (BST)
Subject: Re: Image Conversion

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Message-Id: {9609110945.AA00354-at-mail.bris.ac.uk}

Oxford Instruments (LINK) AN10000 *.IM images can be readily converted to
TIFF images using NIH Image on Macs. There is a facility for stripping
the 512byte header from the *.IM image. I think it is necessary to invert
the LUT to generate a positive image which can then be saved as TIFF and
transported to PC if necessary.

I do not know if ImagePC - referred to in Dave Teter's message a couple of
days ago - will accomplish the same conversion without the need to use a Mac.

Only problem with this route is that I don't think there is a way of
translating a LINK generated LUT at the same time - but most of the time
this is probably not relevant.

Cheers,
Stu

--
**************************************************************************
Stuart L. Kearns
Electron Microbeam Laboratories
Dept. Of Geology tel: +44 (0)117 928 8204
University of Bristol fax: +44 (0)117 925 3385
Bristol UK BS8 1RJ e-mail Stuart.Kearns-at-bristol.ac.uk
***************************************************************************




From: geosclmr-at-showme.missouri.edu (Lou Ross)
Date: Wed, 11 Sep 1996 08:33:45 -0500
Subject: Kevex 4505P

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Thanks to all for the useful leads to locate this component.

Lou Ross

Electron Beam Analytical Facility
101 Geological Sciences Bldg.
University of Missouri
Columbia, MO 65211
(573) 882-4777, 882=5458 fax

http://www.missouri.edu/~geosclmr/ebaf.html

'Hindsight... foresight...
sometimes we have no sight at all.'






From: Jacob Wilbrink :      102677.1135-at-CompuServe.COM
Date: 11 Sep 96 12:43:43 EDT
Subject: Please subscribe

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I would like to subscribe to the Microscopy Bulletin board.

Thanks

Jacob






From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 11 Sep 96 12:25:49 EDT
Subject: Image Processing

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Message-id: {32209565-at-dancer.Dartmouth.EDU}

I scan my negatives on a LaCie Silverscanner III fitted with an Epsom
Transparency Adapter. With a good digital printer the results are closing in on
photographic quality .
Kate Connolly




From: psic-at-uclink4.berkeley.edu (Paula Sicurello)
Date: Wed, 11 Sep 1996 09:39:22 -0700 (PDT)
Subject: Polaron Sputter Coater

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I have a PolaronE5400 sputter coater with a Polaron E5500/07 film thickness
monitor. I can't get the thickness monitor to zero and I can't find any
instruction book. Can anybody out there help me with either instructions
or a phone number to Polaron?

Paula
psic-at-uclink4.berkeley.edu






From: John Baltrus :      BALTRUS-at-virago.petc.doe.gov
Date: Wed, 11 Sep 1996 13:47:10 -0400
Subject: Presider Needed Microsopy Session Pittcon '97

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Message-Id: {s236c2f5.067-at-virago.petc.doe.gov}
X-Mailer: Novell GroupWise 4.1

I am currently helping to arrange program sessions for the Pittsburgh
Conference in Atlanta on March 16-21, 1997.

I am looking for a volunteer who will be attending the Conference to
preside at a session titled: "Microscopy/Bioanalytical Microscopy". The
session includes a variety of microscopies and many are applied to
bioanalytical research. The session will be on Wednesday afternoon,
March 20, 1997.

Please direct your responses to me by September 23rd at
baltrus-at-petc.doe.gov

Thanks!

John P. Baltrus





From: richard.lander-at-stonebow.otago.ac.nz (Richard Lander)
Date: Thu, 12 Sep 1996 08:33:58 +1200
Subject: Zerostat guns

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Charles McLaughlin has asked me to post this message:

I have been told that Zerostat anti-static guns (for removing static during
sectioning and cryosectioning) are no longer commercially available. Has
anyone found an alternative method of eliminating static during
cryoultramicrotomy?

Thanks in advance,

rich



Richard Lander
South Campus Electron MicroscopeUnit
c/- Pathology Department
Otago Medical School
P.O. Box 913Dunedin
N.Z.
Tel. National 03 479 7301 Fax. National 03 479 7254

"Southernmost EM Unit in the World!"






From: lkerr-at-mbl.edu (Louis Kerr)
Date: Wed, 11 Sep 1996 14:07:32 -0400
Subject: Re: Fiber-Optic Ring Illuminator

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Try Joan Fulton at:

Fostec, Inc.
62 Columbus Street
Auburn, NY 13201-0275
315-255-2791
FAX 315-255-2695

They produce fiber optic devices including what you are asking about.

Louie Kerr


At 10:54 AM 9/11/96, cxhx-at-MUSICA.MCGILL.CA wrote:
} Would anyone be able to relay the coordinates of a supplier for a
}
} miniature fiber-optic ring illuminator
}
} that fits around a microscope objective (objective ~ 20 mm diameter) with
} fosuses 3.8 to 14 mm.
}
} For the purpose of creating darkfield on an inverted microscope.
}
} Paul Heroux
} McGill Medicine

Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)

VISIT OUR WEB SITE:
http://www.mbl.edu






From: Microscopy-request
Date: Thursday, September 12, 1996 8:33AM
Subject: Zerostat guns

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Charles McLaughlin has asked me to post this message:

I have been told that Zerostat anti-static guns (for removing static during
sectioning and cryosectioning) are no longer commercially available. Has
anyone found an alternative method of eliminating static during
cryoultramicrotomy?

Thanks in advance,

rich



Richard Lander
South Campus Electron MicroscopeUnit
c/- Pathology Department
Otago Medical School
P.O. Box 913Dunedin
N.Z.
Tel. National 03 479 7301 Fax. National 03 479 7254

"Southernmost EM Unit in the World!"






From: Kirk Rogers :      rogers-at-er6.eng.ohio-state.edu
Date: Wed, 11 Sep 1996 13:21:36 -0500
Subject: macs in science and engineering mailing list announcement

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NIH-Image {nih-image-at-soils.umn.edu}
Message-id: {AE5C709A-E1E60-at-164.107.184.182}
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Announcement:

I am starting a Macs in Science and Engineering - Mailing List

Purpose:
To quickly disseminate information about and related to science and
engineering. This includes but is not limited to software
announcements/reviews, programming, finding software to suit a particular
purpose, application, MacOS and instrumentation help, etc. Postings also
include MacOS evangelism and employment opportunities.

To subscribe, send a message to {macjordomo-at-mse59.eng.ohio-state.edu} , with
the commands in the *BODY* of the message. You can send multiple commands,
each in one line finishing with END.

SUBSCRIBE MacSciEng-List Your_FirstName Your_LastName

UNSUBSCRIBE MacSciEng-List

SET List_Name OPTION
Sets your subscription parameters to OPTION

ACKN : Confirms that you sent a message to the list.
NOACKN : No Acknowledgment is sent
[Default].

DIGEST : Sends digests rather than individual messages
MAIL : Sends you individual messages.
[Default].

If you have problems, contact {Postmaster-at-mse59.eng.ohio-state.edu} or
{rogers-at-er6.eng.ohio-state.edu} .

If there is sufficient interest, I will make a searchable archive of the
messages, and a FAQ.








---------------------------------------------------
This message was created and sent using the Cyberdog Mail System
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From: A. Kent Christensen :      akc-at-umich.edu
Date: Wed, 11 Sep 1996 14:40:41 -0400 (EDT)
Subject: Re: EM-Image Processing

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Yuhui,

The combinations of equipment used for digital processing of EM negatives
will vary greatly from lab to lab. We have obtained excellent results
with the following combination:

1. Scan the EM negatives with a Leafscan 45 scanner (Leaf Systems, Inc.,
250 Turnpike Rd, Southboro, MA 01772; (508) 460-8300 -- however I think
they were bought out by some parent company). This can give very high
resolution (with consequent large files), taken up directly in Adobe
Photoshop on a Power Mac 8500. We learned about the Leafscan 45 from
Martin Muller's lab in Zurich.

2. Work with the EM files on the Mac with Adobe Photoshop 3.0 (crop,
brightness, contrast, size, resolution, grouping, labeling, and so forth
to infinity).

3. When the micrograph is exactly as you want it, print it from the
Macintosh on a Kodak XLS 8600 PS Printer. The results are glossy prints
that can rival anything you could do in the darkroom. The printer can
print B&W or color with very good quality. The "printing paper", however,
is quite expensive.

Kent

A. Kent Christensen
Department of Anatomy and Cell Biology
University of Michigan Medical School
{akc-at-umich.edu}

--------------------------------------------

On Wed, 11 Sep 1996, yuhui xu wrote:

} Dear Colleages:
}
} I will appreciate it very much if anyone of you could tell me whether there
} are photo scanners on the market that can be used to scan EM negatives into
} the computer? I was told by a person from an image processing company that it
} is possible to do this by using a transparency device. But I need to know if
} there are people who are actually using those products for this purpose. I am
} also interested in knowing the softwares, and printers that use photographic
} paper. I do know there have been similar products that can be used for light
} microscopy, e.g.,for flurescence microscopy, where the resolution of the
} image is not a big concern. I do not know whether they can be used for
} electron microscopy.
}
} Thanks.
}
} Yuhui Xu,MD,PhD
} DFCI Core EM Facility
} Harvard Medical School
}





From: Robert Fisher :      rmfisher-at-u.washington.edu
Date: Wed, 11 Sep 1996 15:49:36 -0700 (PDT)
Subject: JEOL 4000 Maintenance

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JEOL 4000 Managers.

I would like to make contact with those of you that
are close observers or actually involved with service
of your microscope with particular emphasis on vacuum
conditions and high voltage stability.

Useful tips on conditioning methods would also be helpful.

Thanks

Bob Fisher





From: Walt Bobrowski (313) 996-7814 :      bobroww-at-aa.wl.com
Date: Wed, 11 Sep 1996 15:18:05 -0400 (EDT)
Subject: RE: Image Processing

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Mr-Received: by mta SRVR05.MUAS; Relayed; Wed, 11 Sep 1996 15:18:05 -0400
Mr-Received: by mta SRVR05; Relayed; Wed, 11 Sep 1996 15:18:05 -0400
Mr-Received: by mta SRVR01; Relayed; Wed, 11 Sep 1996 15:21:48 -0400
Disclose-Recipients: prohibited

IMHO,

For photographic quality of a digital image, the only way to go for output is
the
Fujix Pictrograph 3000 digital printer (~$25K). With a top notch electronic
image, you'dswear it was a photo.

Best regards,

Walt Bobrowski
Parke-Davis Pharmaceutical Research





From: Norm Olson :      nho-at-holli.com
Date: Wed, 11 Sep 1996 22:07:25 -0500 (EST)
Subject: Cryoelectron Microscopy Shortcourse

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Cryoelectron Microscopy Shortcourse

Purdue University is offering an intensive, four-day workshop
in the basics of cryo-electron microscopy. The course will run
from November 3 through November 6 and will include theoretical
discussions and hands-on demonstrations in preparing
vitrified samples of biological macromolecules. Students will also learn
low-dose, phase-contrast imaging procedures and image recording on
both film and CCD cameras. An introduction to the methods of
image analysis and three-dimensional image reconstruction will
also be given. For registration information contact: Susan Umberger,
7136-U, Purdue University, Division of Conferences,
1586 Stewart Center, Room 116, West Lafayette, Indiana 47907-1586.
Phone: 317-494-7217. For course content information see the
web site at http://bilbo.bio.purdue.edu/~workshop/ or contact
workshop-at-bilbo.bio.purdue.edu. The workshop fee is $1200 US or
$1350 after September 15 and includes course materials, lunches, a
banquet and lodging.





From: Jay Jones :      JONESJ-at-ULVACS.ULAVERNE.EDU
Date: Wed, 11 Sep 1996 19:49:32 -0800 (PST)
Subject: Help finding old Vanox accessories

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In an effort to aquire a research grade photomicroscope on a spartan budget,
I puchased a 70's vintage Vanox model S (I am assuming this since there is
no model number on the scope.) It came with a fully functional PM 10 CBAD and
a 4x5 camera body. The auction purchase price was about $3K and it came with
a full array of Neo S Plan optics.

The scope is great but it is set up for reflectance microscopy. This has
worked very well for reflectance work but most of our needs are for
transmission. I thought that we could purchase the transmission optics with
little difficulty. This has not been the case. Olympus no longer supports the
scope and efforts to find the needed parts have not yeilded any leads.

Does anyone know where I might obtain the substage components for standard
light field/ darkfield. Phase would be nice too but the former (at a
reasonable price) would save the day.

As an alternative we would certainly entertain liquidiating this scope for
something that is closer to our needs. The scope is in superb condition!

Any leads would be greatly appreciated.

Jay Jones
****************************************************************************
Jay H. Jones Internet: jonesj-at-ulvacs.ulaverne.edu
Professor of Biology and Biochemistry
Biology Dept.
University of La Verne 909 593-3511 x4040 office
La Verne, CA 91750 909 593-3511 x4604 lab
****************************************************************************





From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Thu, 12 Sep 1996 15:33:43 +1000 (EST)
Subject: Re: EM-Image Processing

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} We have Microtek scanners:
} A 45 T for negatives up to 4x5 inches, 30 bit color or B&W
} A Scanmaker III for refection scanning; 36 bit color or B&W
} a 35 t for fast scanning of 35 mmm size transparencies
}
} We scan in the images so they are at least one megabyte (say 1024x1024).
We process the digital images in Photoshop 3.0 on a 100 mHz Pentium with
128 Mb of RAM and a 1.6 Gb disk.
}
} For advanced enhancement we use a PiXision workstation.
}
} But most of our images are now recorded digitally from the start. We
archive them on CD ROM.
}
} For page makeup we mostly use CorelDraw.
}
} For output we use a 600 DPI HP Laserjet for draft quality and a Tektronix
440 dye sublimation printer for photorealistic output. It does excellent
monochrome and briliant color, Cost AU$3.00 a page for mon o and AU$5.00
for color.
}
} The system has been very well received by our users. The final result is
very professional and takes much less skill than conventional photography.
}
} Mel Dickson.
}





From: marilyn-at-cemmsa.adelaide.edu.au (Marilyn Henderson)
Date: Thu, 12 Sep 1996 15:46:55 +0900
Subject: Reference

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Message-Id: {199609120623.GAA26919-at-traminer.cemmsa.adelaide.edu.au}
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Could anyone help me with the following reference details for a colleague?


Bates, A. and Buthala,? (1987). Biological specimen preparation for SEM by
a method other than CPD. Proceedings of the 45th American EM Society of
America, 1987. pp?????



Thanks
Marilyn

Dr. Marilyn Henderson
CEMMSA
The University of Adelaide
South Australia 5005






From: Leszek Kepinski :      kepinski-at-highscreen.int.pan.wroc.pl
Date: Thu, 12 Sep 1996 09:40:07 +0200
Subject: Advanced Imaging Tech.

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Message-ID: {3237BE57.5FDA-at-highscreen.int.pan.wroc.pl}

Hi.
Are there lectures presented at the workshop :"Advanced Imaging
Techniques Applied to Catalyst Characterization", Baltimore 1996,
available in printed or electronic form?

Leszek Kepinski

====================================================================
| Address: Dr. Leszek Kepinski, |
| Institute of Low Temperature and Structure Research, |
| Polish Academy of Sciences, |
| ul. Okolna 2, P.O. Box 937, |
| 50-950 WROCLAW 2, Poland. |
| phone: 48(71) 3435021 ext. 153; fax: 48(71) 441029. |
| e-mail: kepinski-at-highscreen.int.pan.wroc.pl |
| www: http://www.int.pan.wroc.pl |
====================================================================




From: Self :      GIRAFFE/EURC
Date: Thu, 12 Sep 1996 08:27:59
Subject: Re: Polaron Sputter Coater

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Forwarded message:

Hello Paula

Regarding your problem with the film thickness monitor, firstly,
the crystal for the film thickness monitor may be saturated or
be shorting. An extract from the instruction book reads:

"Quartz crystal: .. it will function normally until
the total thickness of deposited material causes the
oscillation frequency to be outside the range of
the measuring system. At this point, which corresponds
to about 11 microns of aluminium or 2 microns of gold,
the digits on the display will no longer alter during
deposition. The crystal must then be changed."

Secondly, the density setting could be wrong. The setting for
gold is 19.4 g/cc, Au/Pd is 18.0 g/cc.

I hope this solves your problem. I could fax you the important
parts of the instruction manual if you can't obtain one from
someone nearer to you.

Regards

Robin


Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)




From: Paolo Ghiara :      ghiara-at-sienanet.it
Date: Thu, 12 Sep 1996 11:41:29 -0700
Subject: Image acquisition

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Message-Id: {32385959.FC1-at-sienanet.it}

Dear friends,
I would greatly appreciate your help in choosing an optimal
configuration for an image aquisition and storage system that I am
trying to finalize.
I am presently using a single CCD camera (Optronics ZVS-47E) with a 2:1
interlaced 525 lines, 30 frames/sec, resolution. I perform image
acquisition through a Kontron framegrabber and I am using KS400 software
for my analyses, using a 486 66MHz, 16Mb ram PC. I am not happy with the
resolution of the images that I store (I do mostly histology and
immunohistochemistry).
I would like to move to a 3CCD camera a better framegrabber and a
Pentium PC. Could you please give me some advice on the following items
I've been examining in the market?
3CCD cameras: JVC KY-F55E or KY-F30BE
Panasonic GP-US502
Hamamatsu C6157
most of the above have 1/3" CCDs
framegrabber: Matrox Millenniun with 2 or 4 MB
Pentium PC 133MHz with PCI structure.
Thank you for your time and advice. You may either answer in the list or
privately at the address: Ghiara-at-sienanet.it
bye
Paolo




From: ebs-at-ebsciences.com
Date: Thu, 12 Sep 1996 06:11:13 -0500
Subject: Re: Polaron Sputter Coater

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Hi Paula-

The "Polaron" products are now manufactured by an English company called VG
Microtech, who purchased the product line from BioRad about 4 years ago. We
(Energy Beam Sciences) are the authorized Polaron distributors in the United
States. We can supply instruction manuals for any Polaron product. We also
have trained service engineers on staff. Please contact me by e-mail or
toll-free telephone (800-992-9037).

Best regards
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: sf-at-noesis.noesis.fr (Serge Foret)
Date: Thu, 12 Sep 1996 14:21:54 +0100
Subject: Re: Image Conversion

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Dear M. Kearns.

} Oxford Instruments (LINK) AN10000 *.IM images can be readily converted to
} TIFF images using NIH Image on Macs.
} There is a facility for stripping the 512byte header from the *.IM image.
There is also the import capability on Visilog 4.

} I think it is necessary to invert
} the LUT to generate a positive image which can then be saved as TIFF and
} transported to PC if necessary.
You could do that under Visilog 4.
Create an image with the same size as your image,
with the grey level 255 inside and subtract your image to this constant image
create("a",255,...);
subtract("a","YOUR_IMAGE","RESULT");

} I do not know if ImagePC - referred to in Dave Teter's message a couple of
} days ago - will accomplish the same conversion without the need to use a Mac.
I don't know.

} Only problem with this route is that I don't think there is a way of
} translating a LINK generated LUT at the same time - but most of the time
} this is probably not relevant.

Do you really want the Pseudo Color LUT, or just to invert the image ?
I think you can import Oxford images directly into Visilog.

PS: Just for your information, Visilog 5 is able to read Oxford Image format,
but it does not yet take LUT into account.

Regards.
-----------------------------------------------------------------
| Serge FORET | Email: Serge.Foret-at-noesis.fr |
| |
| NOESIS S.A. Immeuble ARIANE |
| Domaine Technologique de Saclay |
| 4 Rue Rene Razel - Saclay |
| F-91892 ORSAY CEDEX |
| France |
| |
| Tel: (33-1) 69 85 55 81 Fax: (33-1) 69 85 52 66 |
| Hotline: (33-1) 69 85 56 45 |
-----------------------------------------------------------------




From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Thu, 12 Sep 1996 09:31:23 -0500
Subject: Bulbs for Durst enlarger

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Message-Id: {199609121322.JAA26870-at-dogwood.botany.uga.edu}
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Hi,
Our lab has a Durst Laborator 138S enlarger. I called Durst for info on
replacing the bulb - for an Atlas 110-120V, 300W, Opale their price is
$63.00. Anyone know of a better deal out there?

Thanks for your help,

Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602






From: John.Tong-at-cor.dowjones.com
Date: Thu, 12 Sep 1996 9:48:14 -0400
Subject: subscript

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Is any one to be able to tellme how to subscript?
Thank you very much

John






From: Audette, David :      deaudette-at-corp.olin.com
Date: Thu, 12 Sep 1996 09:05:00 -0500
Subject: Wanted: Used Ultracentrifuge

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Does anyone have a used ultracentrifuge, in good operating condition, that
is for sale?

Gwen Gifford
Olin Research Center
Cheshire, CT
gegifford-at-corp.olin.com




From: tania-at-dynamotive.com (Tania Jones)
Date: Thu, 12 Sep 1996 08:40:02 -0700
Subject: JEOL service numbers

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Hello,

I am trying to contact the JEOL service representatives in Canada but
unfortunately, the number I have doesn't seem to work anymore. If anyone
could pass along the number, I'd be grateful.

Thanks in advance,

Tania Jones





From: Megill John R :      Megill_John_R.PRILVMS3-at-msmail.bms.com
Date: Thu, 12 Sep 1996 14:00:58 -0400
Subject: RE: Bulbs for Durst enlarger

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Message-ID: {199609121439.JAA11556-at-IndyNet.indy.net}
To: SPM List {spm-at-di.com} , Microscopy list {microscopy-at-Sparc5.Microscopy.Com}
microscopy-at-sparc5.microscopy.com
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Content-transfer-encoding: 7BIT

Beth,

Check with a local Photo Supplier. Prefferally a large one that deals with
enlargers.




From: Nina S Allen :      nallen-at-unity.ncsu.edu
Date: Thu, 12 Sep 1996 12:42:15 -0400 (EDT)
Subject: RE: Image Processing

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I too have the Fujix and our pictures really are excellent (if I say so
myself). Nina Allen

On Wed, 11 Sep 1996, Walt Bobrowski (313) 996-7814 wrote:

} IMHO,
}
} For photographic quality of a digital image, the only way to go for output is
} the
} Fujix Pictrograph 3000 digital printer (~$25K). With a top notch electronic
} image, you'dswear it was a photo.
}
} Best regards,
}
} Walt Bobrowski
} Parke-Davis Pharmaceutical Research
}
}




From: Leo Marin :      leo-at-spine.med.utoronto.ca
Date: Thu, 12 Sep 1996 09:07:05 -0400 (EDT)
Subject: Re: Zerostat guns

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Hi Richard,
For regular ultra-microtoming I use a anti-static gun which is
manufactured or supplied by Chapman of Portland, Maine.

Leo Marin

On Thu, 12 Sep 1996, Richard Lander wrote:

} Charles McLaughlin has asked me to post this message:
}
} I have been told that Zerostat anti-static guns (for removing static during
} sectioning and cryosectioning) are no longer commercially available. Has
} anyone found an alternative method of eliminating static during
} cryoultramicrotomy?
}
} Thanks in advance,
}
} rich
}
}
}
} Richard Lander
} South Campus Electron MicroscopeUnit
} c/- Pathology Department
} Otago Medical School
} P.O. Box 913Dunedin
} N.Z.
} Tel. National 03 479 7301 Fax. National 03 479 7254
}
} "Southernmost EM Unit in the World!"
}
}
}




From: Walter A. Mannheimer :      wamann-at-metalmat.ufrj.br
Date: Thu, 12 Sep 1996 11:41:39 EST3EDT
Subject: SEM explosion?

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Fellow Microscopists, need your help for following puzzle:

We operate a scanning electron microscope (make and model withheld),
tungsten filament, turbomolecular pump mechanically backed, with EDS
beryllium window, about one year old. The instrument is overnight on
vacuum. A few days ago when arrived in the lab in the morning found
that chamber door wide open. The opening was evidently very violent:
the plastic latch was broken, and the door had swung 180 degrees
against a stop, severely bending and damaging detector and stage motor
housings, and putting the door out of alignment. This would indicate
an internal explosion, yes?

We have not yet determined if damage to column or EDS window occurred.
When the turbo pump (which seems to be undamaged) was disassembled,
found that the wire protection screen was thoroughly wetted with a
liquid which feels and smells as alcohol. The pump support had an oily
film mixed with a gritty material. This material is currently being
analyzed. The only place where there is oil in the system is in the
mechanical pump. The hose from this pump is clean, indicating no back
leakage.

The operation routine of the instrument is very careful, mostly
geological samples, no open access, no alcohol used anywhere in the
routine of the lab.

Does anyone recall a similar experience or have any clues? Thanks to
all
Prof.Walter A.Mannheimer
Metallurgy and Materials Engineering
Federal University of Rio de Janeiro
POBox 68505 21945 Rio de Janeiro, Brazil
Voice +5521 280-7443 (Dept.office) +5521 590-0579 (direct)
Fax +5521 290-6626 Email: wamann-at-metalmat.ufrj.br




From: Woody.N.White-at-mcdermott.com
Date: 9/11/96 11:39 AM
Subject: Polaron Sputter Coater

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Could be your crystal is too heavily coated to work properly....

Polaron is currently represented by: Energy Beam Sciences, Inc.
Vioce - (800) 992-9037 (413) 786-9322 Fax: (413) 789-2786

P.O.. Box 468
Agawam, MA 01001


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I have a PolaronE5400 sputter coater with a Polaron E5500/07 film thickness
monitor. I can't get the thickness monitor to zero and I can't find any
instruction book. Can anybody out there help me with either instructions
or a phone number to Polaron?

Paula
psic-at-uclink4.berkeley.edu




From: jleclef-at-snoopy.hypercon.com (Jean Leclef)
Date: Fri, 13 Sep 1996 01:58:34 -0500
Subject: subscribe

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subscribe





From: ebs-at-ebsciences.com
Date: Fri, 13 Sep 1996 05:53:40 -0500
Subject: Re: JEOL service numbers

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Hi Tania-

For JEOL service and sales in Canada, you should contact Soquelec Ltee. Ltd.
in Montreal. Their telephone number is 514-482-6427 and their fax number is
514-482-1929. I'm sure that Jean-Pierre Slakmon and his staff will be able
to help you.

Best regards,
Steven E. Slap
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: Crossman, Harold :      crossman-at-rd.sylvania.com
Date: Fri, 13 Sep 1996 08:26:00 -0400
Subject: cleaning standard

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Message-Id: {c=US%a=_%p=SYLVANIA%l=SYLVANIA/OSI/000139E0-at-da-exc1.sylvania.com}

To all the helpful microscopists on the list:

Thanks for the input on where to obtain MIL-STD-1246C. I haven't
received it yet but will report later.

I bought a copy ($12 plus postage) from:

Document Center Inc.
1504 Industrial Way- Unit 9
Belmont, CA 94002
(415) 591-7600

http://www.service.com/doccenter/home.html

-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.osram.de
www.siemens.com




From: Leo Marin :      leo-at-spine.med.utoronto.ca
Date: Thu, 12 Sep 1996 09:07:05 -0400 (EDT)
Subject: Re: Zerostat guns

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Message-Id: {n1369522906.19442-at-QuickMail.Yale.edu}
"Richard Lander" {richard.lander-at-stonebow.otago.ac.nz}
Cc: "Microscopy-at-Sparc5.Microscopy.Co" {Microscopy-at-Sparc5.Microscopy.Com}
X-Mailer: Mail*Link SMTP-QM 3.0.3 b1 d5

RE} } Zerostat guns 9/13/96

Richard,
You may want to try the Static Line II made by diatome. It is an anti static
line that can be used during sectioning and for cryoultramicrotomy. We bought
ours through Electron Microscopy Sciences.
Linda Iadarola
Center for Cell Imaging
Yale University, New Haven, CT

--------------------------------------

Leo Marin

On Thu, 12 Sep 1996, Richard Lander wrote:

} Charles McLaughlin has asked me to post this message:
}
} I have been told that Zerostat anti-static guns (for removing static during
} sectioning and cryosectioning) are no longer commercially available. Has
} anyone found an alternative method of eliminating static during
} cryoultramicrotomy?
}
} Thanks in advance,
}
} rich
}
}
}
} Richard Lander
} South Campus Electron MicroscopeUnit
} c/- Pathology Department
} Otago Medical School
} P.O. Box 913Dunedin
} N.Z.
} Tel. National 03 479 7301 Fax. National 03 479 7254
}
} "Southernmost EM Unit in the World!"
}
}
}

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From: cacharro-at-mpiz-koeln.mpg.de (Jorge Cacharron)
Date: Fri, 13 Sep 1996 16:37:40 +0100
Subject: Re: Zerostat guns

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Date: Fri, 13 Sep 1996 16:38:36 +0100
Subject: unsubscribe

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From: Robin Griffin :      rgriffin-at-eng.uab.edu
Date: Fri, 13 Sep 1996 07:47:47 -0500
Subject: filament and TEM gun protection

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Message-ID: {c=US%a=_%p=UAB%l=ENGEM0-960913124747Z-4347-at-engem0.eng.uab.edu}

After being off maintenance contract for two years on our JEOL 2000FX
(200kV) TEM, we've returned to the fold. I've just found out that JEOL
refuses to guarantee my gun under the maintenance contract if I don't
use filaments that they specify as OK. If their is a problem with the
gun that they can prove arises from the filament, they won't fix it.
The filaments they support are about a factor of three times more
expensive than what I have in stock. I would like to use up my supply
of cheaper filaments. Am I taking a big risk? Does a lower quality
filament really risk the health of a gun? I've always been satisfied
with the brightness, stability, and life span of these filaments in the
past.




From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 13 Sep 1996 13:33:52 -0400 (EDT)
Subject: Re: SEM explosion?

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}
} Fellow Microscopists, need your help for following puzzle:
}
} We operate a scanning electron microscope (make and model withheld),
} tungsten filament, turbomolecular pump mechanically backed, with EDS
} beryllium window, about one year old. The instrument is overnight on
} vacuum. A few days ago when arrived in the lab in the morning found
} that chamber door wide open. The opening was evidently very violent:
} the plastic latch was broken, and the door had swung 180 degrees
} against a stop, severely bending and damaging detector and stage motor
} housings, and putting the door out of alignment. This would indicate
} an internal explosion, yes?
}
Dear Walter,
Yes, and the two types which come to mind would be chemical and
sudden evaporation/sublimation of a liquid/solid. In the first case,
there would have to be both an inflammable material and a reactant
(usually an oxidising agent) which would mix in the chamber. Had this
happened, I would expect that there would be residue from incomplete
combustion; i.e., there would be more than an alcohol-like residue on
the TMP screen. It is possible that hydrogen and oxygen somehow got into
the chamber, in which case there would be no residue; this is unlikely
barring sabotage, but oxygen can condense at 77 K, so there is a conceiv-
able mechanism for getting liquid oxygen into the system.
LN2 does not evaporate suddenly, but perhaps other cryo-liquids
do. In this case, the sudden expansion would cause an increase in pres-
sure without leaving a residue. Failure of a valve on a compressed gas
tank would also do this, but that would have left obvious clues.
Yours,
Bill Tivol





From: Crossman, Harold :      crossman-at-RD.SYLVANIA.com
Date: Fri, 13 Sep 1996 15:11:00 -0400
Subject: re: sem explosion?

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Message-Id: {199609131655.MAA02760-at-thomas.ge.com}

My two cents from a failure analysis lab:

To go out on a limb...

(Assuming a N2 line exists)
Is the chamber vented with N2 through a pressure reduction stage? I'm
wondering if there could have been a failure in the pressure reducer
allowing high pressure gas to fill the chamber. This would have given
the appearance of an explosion and would have stirred things up a bit,
maybe even adding some of that debris you describe. Gas lines can have
traps for oil, pipe grit, etc. even when they're not supposed to need
them. A sudden rush of gas could have blown stuff downstream. Stranger
things have happened.

Was there any maintenance being performed on the N2 plumbing? Was the
reservoir being filled? Emptied? What is/was the line pressure? Does
the valve/pressure reducer have a handle that can be turned easily?

Could the oil have been from the bearing lubrication system if the turbo
did not shut down immediately? If the turbo controller has a power
supply with a fault indicator, was it lit?


-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.osram.de
www.siemens.com








From: Woody.N.White-at-mcdermott.com
Date: 9/12/96 11:41 AM
Subject: SEM explosion?

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In over 15 years of SEMing, have not heard of such... will be
interesting to see other replies.

First question the comes to mind... Does the EDS dewar still contain
liquid nitrogen?

Woody White


______________________________ Reply Separator _________________________________


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Fellow Microscopists, need your help for following puzzle:
...snip...This would indicate an internal explosion, yes?

We have not yet determined if damage to column or EDS window occurred.
...snip...

Does anyone recall a similar experience or have any clues? Thanks to
all
Prof.Walter A.Mannheimer
Metallurgy and Materials Engineering
Federal University of Rio de Janeiro
POBox 68505 21945 Rio de Janeiro, Brazil
Voice +5521 280-7443 (Dept.office) +5521 590-0579 (direct)
Fax +5521 290-6626 Email: wamann-at-metalmat.ufrj.br




From: a114-at-lehigh.edu
Date: Fri, 13 Sep 1996 17:03:17 EDT
Subject: re:fumed silica

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} Date: Fri, 13 Sep 96 12:55:14 EDT
} From: "MARK DARUS (216) 266-2895 GENERAL ELECTRIC CO." {darus-at-cle.dnet.ge.com}
} Subject: Fumed Silica sample prep.
}
} Does anyone have a suggestion as to how I can prepare fumed silica for
} analysis under an SEM. My problem is that the silica won't stick to
} carbon tape, only as small particles. I would like to get a fairly even layer.

You might try collecting the particles on a Nuclepore filter.

Michael Zemyan




From: GVKM07A-at-PRODIGY.COM (DR CHARLES A GARBER)
Date: 13 Sep 96
Subject: Fumed Silica sample prep.for SEM

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Message-Id: {199609132246.SAA12406-at-mime2.prodigy.com}
X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc04

-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #3.0 ] --

Mark Darus wrote:
} ====================================================
} } Does anyone have a suggestion as to how I can prepare fumed silica
for
} } analysis under an SEM. My problem is that the silica won't stick to
carbon
} } tape, only as small particles. I would like to get a fairly even
layer.
} ====================================================
} This might sound a bit involved and drawn out, but this is a
procedure that we
} have used for particles in this range, including fumed
} silica, where the basic particles really are on the order of nm sizes.
I
} publicized this method about a year go, it was taught to me by Robert
P. Schatz
} who is now deceased, when we were both working at the DuPont
Experimental
} Station in the late 1960's and sharing a laboratory, I am not sure if
the
} method was his own innovation, but he sure did know how to recognize a
system
} that could benefit from this method.
}
} Mix up some camphor (60%) and napthalene (40%) which is then heated to
melting
} (which occurs not too far above room temperature) where the two
compounds form
} a very interesting (for EM people) eutectic. The freezing point is
just a few
} degrees above room temperature, so the idea is to make the liquid
solution of
} the two compounds, then add some of the powder to be studied, and then
with an
} eye dropper, put a few drops onto a glass slide or cover slip. The
liquid of
} course instantly freezes, and at the same time, immobilized fine
particles in
} 3D space.
}
} Then put the substrate with the thin layer of solid eutectic into a
vacuum
} evaporator and let it pump with mechanical pump vacuum over night.
The solid
} sublimes away, so that by morning, the colloidal particles of silica
(or
} whatever else one might be studying) is uniformly dispersed on the
substrate.
} If the dispersed particles are of SEM size, then they can be studied
directly
} by SEM. If more apprpriate for TEM observation, then the particles
are all
} ready to be Pt/C replicated and then studied. If one sees "doublets"
then they
} were probably doublets to begin with and were not dublets that were
formed
} during sample preparation.
}
} Any ordinary camphor or naphthalene will do, nothing special otherwise
is
} needed, they can be purchased from any reputable scientific supply
house.
}
} Chuck
}
======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.
com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.
com


Look for us!
############################
WWW: http://www.2spi.com
############################
=====================================================




From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Fri, 13 Sep 1996 18:59:48 -0500
Subject: SEM Explosion Another Idea

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Walter

The clues you gave also point to the possibility of
a high pressure compressed air line failure. Do you use "house"
compressed air to for example operate any pneumatic valves
on your SEM.

Frequently these air lines contain "oil" and other oily fluids.
Certainly, the compressed "house" air lines that we use here at ANL to
operate all valves and provide several Atmospheres of pressure. I
think I would investigate the (slim ) possiblity of
one of your pneumatic valves failing internally with
a subsequent dump of the high pressure into the pumping
lines. This would overpressurize the colum and certainly cause
the door to fling open. But you should have heard the air
line releasing pressure into the room when you walked in, unless the
compressor died too. I guess my message is to check your
Pressurized piping and all valves too.

Nestor
Your Friendly Neighborhood SysOp.






From: George.C.Ruben-at-Dartmouth.EDU (George C. Ruben)
Date: 14 Sep 96 07:55:34 EDT
Subject: Re: Fumed Silica sample prep.for SEM

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Message-id: {17875169-at-prancer.Dartmouth.EDU}
Microscopy-at-Sparc5.Microscopy.Com

Chuck,

Your method for dispersing particles on
a surface using a 60% camphor/40% napthalene particle suspension eutectic is
equivalent to freeze-drying except you don't need the fancy cold stages and
high vacuum apparatus. Both camphor and napthalene will sublime at room
temperature on the bench or in a vacuum system leaving the particles behind on
a selected surface.

George C. Ruben
Dartmouth College




From: koht-at-usa.pipeline.com (Craig A. Koht)
Date: Sat, 14 Sep 1996 17:41:11 GMT
Subject: Re: [Fwd: filament and TEM gun protection]

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Hey Mike,
Thanks for the mail forwarding, especially the first regarding 2000
filament/SC policy.
I'll forward same to Pat on Monday, hopefully someone in SSG has
intercepted same message.
The context that this was presented (I'm back in the S/C fold and being
"extorted into high priced filaments") has to be followed with a phone call
to customer with a more understanding and practical discussion/solution.
Thks & Have a good weekend !
Craig

PS I'll be in Boston on Tueday - I'll find out more about above.




From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Mon, 16 Sep 1996 09:59:33 +0100 (BST)
Subject: Re: Fumed Silica sample prep.

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On Fri, 13 Sep 96 12:55:14 EDT "MARK DARUS (216)
266-2895 GENERAL ELECTRIC CO."
{darus-at-cle.dnet.ge.com} wrote:
} Does anyone have a suggestion as to how I can prepare
fumed silica for
} analysis under an SEM. My problem is that the silica
won't stick to
} carbon tape, only as small particles. I would like to get a
fairly even layer.

I've had some success with the following method. Make a
very thin suspension in an inert liquid such as 2-propanol
and dry a drop on a smooth glass surface (e.g. a
microscope slide or cover slip). You will find that with
particles as small as silica fume the surface forces are
strong enough to hold them on the glass. The effect is
stronger than if you just dry-dust the material onto the
substrate.

Dr Eric Lachowski
University of Aberdeen
Department of Chemistry
Aberdeen, Scotland
tel +44 1224 272934
fax +44 1224 272921





From: ryna.marinenko-at-nist.gov (Ryna Marinenko)
Date: Mon, 16 Sep 1996 10:24:14 -0500
Subject: MAMAS Meeting

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Attached is a technical meeting announcement


MAMAS
(Mid-Atlantic Microbeam Analysis Society)
and the
Surface and Microanalysis Science Division,
NIST

Meeting
at the
National Institute of Standards and Technology
Gaithersburg, MD
on
Monday, October 7, 1996
10:30 am- 3:00 PM
Lecture Room D, Administration Bldg.

10:30 Coffee and Doughnuts

10:45 Wilbur Bigelow, MAS Tour Speaker, Prof. Emeritus,
U. Michigan
"Some Fundamental Reasons Why it Takes
So Long to Pump Down to a High Vacuum"

12 noon Lunch

1:00 Gene Jarosewich, Department of Mineral Sciences,
Smithsonian Institution

"Standards for Geological Analysis"
2:00 Wilbur Bigelow
" Why and How Oil Diffusion Pumps are being
Replaced in Electron Beam Instruments"



For more information, contact Ryna Marinenko (301)975-3901,
FAX(301)216-1134, email:ryna.marinenko-at-nist.gov


Ryna Marinenko
NIST
Rm A113, Bldg 222
Gaithersburg, MD 20899-0001
FAX: 417-1321
email: ryna.marinenko-at-nist.gov






From: mrdunlap-at-ucdavis.edu (Michael Dunlap)
Date: Mon, 16 Sep 1996 09:51:30 -0700
Subject: Re: SEM explosion?

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I have not hear of a SEM exploding but I could conceive of it happening.
If Liquid oxygen, a carbon source and a metal catalysis are mixed together
an explosion could occur. If your EDS system was cold enough to cool O2 to
a liquid. The liquid O2 could come in contact with vacuum grease and there
is plenty of different metals to act as a catalysis. Although, very rare,
I have hear of this happening on other LN2 cool instruments.

Mike

===========================================================
Michael Dunlap lab (916) 752-0284
Facility For Advanced Instrumentation fax (510) 422-2282
University of California mrdunlap-at-ucdavis.edu
Davis CA, 95616
http://carbon.ucdavis.edu
============================================================






From: beth-at-dogwood.botany.uga.edu (Beth Richardson)
Date: Mon, 16 Sep 1996 16:41:29 -0500
Subject: thanks for bulb info

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Message-Id: {199609161743.NAA27890-at-thomas.ge.com}

Originally, I had called Durst for a replacement bulb for a 138S enlarger.
I told them I had an Atlas 110-120V, 200W bulb. I was told that I needed a
300W bulb for that enlarger (even though it says max200W on the enlarger?
go figure) anyway, and that it would cost me $63.00.

I want to thank everyone who replied with names and numbers of their
favorite bulb companies.

Results - The favorite place to bulb shop is PSC Lamps, Inc., (800) 772-5267.

They have a new address:
PSC Lamps, Inc.
1 Fishers Rd.
Pittsford, NY 14534

and a web site - http://www.roccplex.com/psclamps
and they had the cheapest price - $2.61

Other companies:

Bulbs Only - very nice and helpful
954 Queen St
Southington, CT 06489
(203) 621-0213

Bulbman, Inc. - very nice and helpful
sorry forgot to get their address
Reno, NV
(800) - 648-1163

Bulbtronics - nice, except they promised to call back with info and didn't
31 Willow Park Ctr
PO 306
Farmingdale, NY 11735
(800) 654-8542

Again, thanks for the helpful suggestions. I don't know if the person I
spoke to at Durst made a mistake or if they are just price gouging. I'll
use PSC.

Best regards,
Beth



Beth Richardson
EM Lab Coordinator
Botany Department
University of Georgia
Athens, GA 30602-7271

phone (706) 542-1790
fax (706) 542-1805
email beth-at-dogwood.botany.uga.edu






From: RonMervis-at-aol.com
Date: Mon, 16 Sep 1996 18:43:53 -0400
Subject: where can I get microtome knives sharpened???

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I need some information...
I have a few microtome knives (15-25 cm long) that need sharpening but I
don't know where to send them in the states...anybody know who does this sort
of stuff???
please reply to:
RonMervis-at-aol.com

thanks in advance....

Ron Mervis, Ph.D.
NeuroMetrix Research, Inc.





From: Khoo Keng Meng :      medp6023-at-leonis.nus.sg
Date: Tue, 17 Sep 1996 11:35:12 +0800 (SST)
Subject: help needed!

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hi!
i am currently working on some immunohistochemistry work and i was
wondering how can i get into the list?
perhaps you can help me there.
thanks.

keng meng
department of biochemistry
faculty of medicine
national university of singapore





From: ROBIN CROSS :      EURC-at-giraffe.ru.ac.za
Date: Tue, 17 Sep 1996 11:07:13 GMT+0200
Subject: JEOL 733 probe upgrade

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We would appreciate information and advice on upgrade
options for the JEOL 733 microprobe. I am aware that many have
been upgraded, some more successfully than others. We would like
to avoid the problems that others may have experienced!

Thanks


Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)




From: lkerr-at-mbl.edu (Louis Kerr)
Date: Tue, 17 Sep 1996 08:53:51 -0400
Subject: Re: where can I get microtome knives sharpened???

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Message-Id: {199609171259.IAA05697-at-uva.pcmail.Virginia.EDU}

Ron,

I have been using C.L. Sturkey, Inc. They are fast and good at a fair price.

C.L. Sturkey, Inc.
1549 Joel Drive
Lebanon, PA 17046
800-274-9446

Good luck,
Louie Kerr

At 6:43 PM 9/16/96, RonMervis-at-aol.com wrote:
} I need some information...
} I have a few microtome knives (15-25 cm long) that need sharpening but I
} don't know where to send them in the states...anybody know who does this sort
} of stuff???
} please reply to:
} RonMervis-at-aol.com
}
} thanks in advance....
}
} Ron Mervis, Ph.D.
} NeuroMetrix Research, Inc.

Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)

VISIT OUR WEB SITE:
http://www.mbl.edu






From: Gregory Argentieri :      Gregory.Argentieri-at-sandoz.com
Date: Tue, 17 Sep 1996 12:23:40 -0500
Subject: Subscribe to Microscopy news

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Message-ID: {323EDE9C.5437-at-sandoz.com}

I would like to subscribe to the microscopy news group

Mail address is Gregory.Argentieri-at-sandoz.com




From: Rajesh Patel :      rpatel-at-UMDNJ.EDU
Date: Tue, 17 Sep 1996 11:51:44 -0400 (EDT)
Subject: lab6 filaments

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Message-Id: {199609171713.MAA28785-at-Sparc5.Microscopy.Com}

We have a Philips 420 TEM and always has a DENKA lab6 filament.
It always seem that we never get enough life out of the DENKA because the
solder joints housing the crystal always crack a bit. Our acuum system is
good. Are there other types that are better? I heard that Kimbell is a
good one.

Raj






From: Delilah W. Irving :      dirving-at-aggie.pw.usda.gov
Date: Tue, 17 Sep 1996 09:14:19 -0700 (PDT)
Subject: digital cameras

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I'm looking for a source of black and white digital cameras (preferably
those companies offering GSA, i.e., government, contracts) to use with a
light microscope image analysis system. I plan to use the camera to
collect images using an ordinary lens as well. If anyone can be of help,
please email back with suppliers or recommendations. Thanks in advance.

Delilah W. Irving tel: 510-559-5653
USDA - ARS - WRRC fax: 510-559-5777
800 Buchanan St. email: dirving-at-pw.usda.gov
Albany, CA 94710






From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Tue, 17 Sep 1996 10:01:52 -0600
Subject: Re: Zerostat guns

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I'm sorry if this is a duplicate mailing. I think my original message
yesterday didn't make it out.

} } I have been told that Zerostat anti-static guns (for removing static during
} } sectioning and cryosectioning) are no longer commercially available. Has
} } anyone found an alternative method of eliminating static during
} } cryoultramicrotomy?

I just checked with Sigma Chemical Company about availability of Zerostat
guns. As of this morning, they have 52 in stock for US$51.95. The Catalog
Number is Z-3000. When these are gone, they will discontinue the item.

John
chandler-at-lamar.ColoState.EDU






From: jm Lett :      jm_lett-at-cidmac.wustl.edu
Date: 17 Sep 1996 11:46:05 +0100
Subject: Re: Knife Sharpening

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Message-ID: {n1369161695.38942-at-CIDMAC.wustl.edu}

In regards to the query from Ron Mervis about microtome knife sharpening:

One of the technicians in our lab has sent knives to the following
reconditioners and was satisfied with the results from both. She doesn't have
current info on prices or turnaround times.

C.L. Sturkey, Inc. (215) 754-7296
646 Kulp Rd., Perkiomenville, PA 18074

Dorn & Hart (708) 832-3843
135 Home Ave., Villa Park, IL 60181

C.L. Sturkey also sells new knives (I'm not sure about Dorn & Hart).

Helpfully,

Jaclynn Lett

Central Institute for the Deaf
St. Louis, MO
jm_Lett-at-cidmac.wustl.edu






From: Dave King (607)757-1248 T37/257-3 Ext DEKING-at-VNET.IBM.COM
Date: 17 Sep 1996 17:21:06 EDT
Subject: Denka Life

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Message-Id: {199609172212.RAA29333-at-Sparc5.Microscopy.Com}

To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}


Raj,

We found those brazed (M3, I recall), Denka tips need to be
heated and cooled slowly. If you are doing that, they should last
per Denka's literature. The brightness drops as they get old.

No more than about a 0.2 amp per 20 second increase is what we
use when firing up or shutting down.

. {
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: Charles K. Karski :      charlesk-at-nationalnet.com
Date: Tue, 17 Sep 1996 19:12:12 -0400
Subject: subscribe

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subscribe charlesk-at-nationalnet.com Charles K. Karski





From: Karen Vaughn :      klv-at-biotech.ufl.edu
Date: Tue, 17 Sep 1996 16:39:26 -0400
Subject: fixative for phospholipid

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Message-Id: {1.5.4.32.19960917203926.0067e0b0-at-biotech.ufl.edu}
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Does anyone know of a fixative called Iodoplatinate?

I am interested in looking at phospholipids in mammalian tissue. Any other
suggestions for a fixative?


----------------------------------------------------------------------------
---------
Karen Vaughn Tel.(904) 392-1184
EM Technician
University of Florida Fax.(904) 846-0251
Electron Microscopy Core Laboratory Email. KLV-at-biotech.ufl.edu
Interdisciplinary Center for Biotechnology Research
http://www.biotech.ufl.edu/~emcl/
214 Bartram Hall
Gainesville, Fl 32611







From: Microscopy-request
Date: Tuesday, September 17, 1996 11:51AM
Subject: lab6 filaments

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We have a Philips 420 TEM and always has a DENKA lab6 filament.
It always seem that we never get enough life out of the DENKA because the
solder joints housing the crystal always crack a bit. Our acuum system is
good. Are there other types that are better? I heard that Kimbell is a
good one.

Raj






From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Tue, 17 Sep 1996 16:39:39 -0500
Subject: Re: lab6 filaments

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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} We have a Philips 420 TEM and always has a DENKA lab6 filament.
} It always seem that we never get enough life out of the DENKA because the
} solder joints housing the crystal always crack a bit. Our acuum system is
} good. Are there other types that are better? I heard that Kimbell is a
} good one.
}
} Raj

I prefer FEI or Kimball Physics. FEI in particular has given us
good customer service.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
University of Illinois
Rm 74 Bevier Hall
905 S. Goodwin Ave.
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu
(217) 355-1143 home

*********** looking for a job again ***********






From: Dascorr-at-aol.com (by way of Nestor J. Zaluzec)
Date: Tue, 17 Sep 1996 21:33:24 -0500
Subject: Polymer analysis

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I would like suggestions for a method and/or procedure for analyzing the
diffusion gradient of sodium and chloride ions that are transported into a
0.25" thick polymer under cathodic protection (a negative potential of -1.0 V
vs. saturated calomel). Ion contents most likely will be less than 1% of
the polymer content (only C, O, H in chains)

David A. Shifler
NSWCCD
Bldg. 60, Code 613
Bethesda, MD
20084-5000
USA

(301) 227-5128
(301) 227-5576 FAX






From: lkerr-at-mbl.edu (Louis Kerr)
Date: Tue, 17 Sep 1996 16:49:05 -0400
Subject: Re: lab6 filaments

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Rajesh,

We have been using a Kimball Physics LaB6 filament in our JEOL JSM-840 SEM
with very good results. It uses a carbon mount. Life time has been at
least as good as the DENKA and we were instructed by Kimball to not use the
preheat function. In other words the filament is brought up from cold each
time we use it. There is some wandering for about 15 minutes but then it
settles down. On our first Kimball the carbon split post burned through
because of our overheating the filament. I have dealt with Barry
Scientific for purchasing (800-348-2257).

Hope this helps,
Louie Kerr

At 11:51 AM 9/17/96, Rajesh Patel wrote:
} We have a Philips 420 TEM and always has a DENKA lab6 filament.
} It always seem that we never get enough life out of the DENKA because the
} solder joints housing the crystal always crack a bit. Our acuum system is
} good. Are there other types that are better? I heard that Kimbell is a
} good one.
}
} Raj

Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)

VISIT OUR WEB SITE:
http://www.mbl.edu






From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 17 Sep 1996 15:15:39 -1000 (HST)
Subject: Need cryo-TEM in Western US

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Aloha, Microscopists!

A colleague at UCLA is looking for a TEM with a cryo stage that she can
use to look at vitreous films containing a million Dalton protein to try
to work out the 3-D structure. Coming to Hawaii to use our cryostage
seems rather out-of-the-way (unless you're looking for a tan), so we would
like to know if there is one nearer to southern Calif., including the
Pacific NW or the SW. Oh, and a freezing device with cryo-transfer, as
well, of course. If you can reply to me, I'll forward messages to her.

Alternative approaches to this problem would also be welcome. Any ideas
out there?

Thank you all in advance!

Mahalo,
Tina

***************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu *
***************************************************************************





From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Wed, 18 Sep 1996 14:34:40 +1000 (EST)
Subject: Re: digital cameras

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We use Sanyo B/W CCD cameras type VC-2524. They come with C-mount adaptors
to mount on microscopes. Resolution is 800x600 picture elements. They need
a 24 V ac supply. CCIR standard 625 TV lines. Output 1.0 vp-p 75 ohms,
composite, BNC connector. They just plug into a frame grabber. Very
satisfactory. They are commonly used for building security surveillance
systems. About US $1000

Mel Dickson
}
}
}
}





From: Tina Carvalho :      tina-at-pbrc.hawaii.edu
Date: Tue, 17 Sep 1996 15:15:39 -1000 (HST)
Subject: Need cryo-TEM in Western US

Contents Retrieved from Microscopy Listserver Archives
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Aloha, Microscopists!

A colleague at UCLA is looking for a TEM with a cryo stage that she can
use to look at vitreous films containing a million Dalton protein to try
to work out the 3-D structure. Coming to Hawaii to use our cryostage
seems rather out-of-the-way (unless you're looking for a tan), so we would
like to know if there is one nearer to southern Calif., including the
Pacific NW or the SW. Oh, and a freezing device with cryo-transfer, as
well, of course. If you can reply to me, I'll forward messages to her.

Alternative approaches to this problem would also be welcome. Any ideas
out there?

Thank you all in advance!

Mahalo,
Tina

***************************************************************************
* Tina (Weatherby) Carvalho * tina-at-pbrc.hawaii.edu *
* Biological Electron Microscope Facility * (808) 956-6251 *
* University of Hawaii at Manoa * http://www.pbrc.hawaii.edu *
***************************************************************************





From: Stuart Kearns :      Stuart.Kearns-at-bristol.ac.uk
Date: Wed, 18 Sep 1996 09:18:23 +0100 (BST)
Subject: Carbon Coater wanted - UK

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Message-Id: {9609180818.AA19596-at-mail.bris.ac.uk}

For all UK based EM labs.

Does anybody have or know of a diffusion/turbo pumped evaporation unit
suitable for carbon coating either gathering dust or available for purchase.
We are currently using a rough pumped 'flash coater' which we
find wholly unsuitable for microanalysis.

Ideally, an Edwards 306 unit or something similar would suit our
requirements but anything will be considered.
In addition to SOME cash (times are hard you know) we can offer WDS
analysis in return.

Thaks for your cooperation

Cheers,
Stu

--
**************************************************************************
Stuart L. Kearns
Electron Microbeam Laboratories
Dept. Of Geology tel: +44 (0)117 928 8204
University of Bristol fax: +44 (0)117 925 3385
Bristol UK BS8 1RJ e-mail Stuart.Kearns-at-bristol.ac.uk
***************************************************************************




From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Wed, 18 Sep 1996 08:56:15 +0100
Subject: Re: lab6 filaments

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} We have a Philips 420 TEM and always has a DENKA lab6 filament.
} It always seem that we never get enough life out of the DENKA because the
} solder joints housing the crystal always crack a bit. Our acuum system is
} good. Are there other types that are better? I heard that Kimbell is a
} good one.
}
} Raj

I used a number of Philips 400 series TEMs with Denka filaments over a
number of years and had no serious problems - in fact the results were
excellent. Four points (sorry if I state the obvious, or tell you
something you already know):

1. You MUST, especialy with a new filament or one that has recently been up
to air, increase the filament heating SLOWLY. For the first few times, I
would recommend taking perhaps 15 minutes to reach saturation point. After
the filament has been run in for 10 hours or so, you can increase the
heat-up rate, but I would still recommend taking 5 minutes.

2. I always used to run the filaments slightly undersaturated. The
saturation point is not as well defined, in my experience, with LaB6
filaments as with W and users familiar with W tend to increase the filament
current until all structure disappears from the filament image. I my
opinion, this is too high (and sometimes much too high) and there is
minimal loss in brightness by operating with structure (perhaps quite a
lot) still apparent in the filament image - this position will generally be
about 3-4 steps lower on the filament control.

3. Check the saturation carefully when changing the gun emission. Even with
W, the saturation point will vary as the bias is changed. This is more
pronounced with LaB6 and more critical. If you turn the emission up, this
is obvious, as the filament de-saturates. The problem usually arises when
users reduce the emission, and forget to reset the filament saturation (if
you follow my suggestion above, this is a less significant issue).

4. Problems also arise if STEM mode is used frequently and the user does
not check the filament image - DO NOT rely on brightness levels to
determine the saturation point, as you will get it wrong. Saturate in TEM
mode first, where you can carefully check the filament image, then switch
to STM.

I would also note that filament alignment is much more critical with LaB6 -
you MUST do it with a stereo microscope.

Also, make sure you are using the correct LaB6-compatible wehnelt
assemblies and wehnelt caps - the caps are much larger than earlier types,
with extra holes to ensure good vacuum near the tip and the wehnelt may
also have additional holes. If you try using the earlier type of wehnelt
(no holes), you will certainly get early failure.

The mode of failure I experienced was exactly as you describe. You don't
actually say what lifetime you are getting. From what I remember, Denka
specify a rather broad range of lifetimes - I would not expect to get
anywhere near the upper end doing analytical TEM. You will only achieve
these very long life times with the filament well back from the wehnelt,
low emission and careful operation - for example, low magnification work on
a SEM. I guess for routine analytical TEM, I would be happy with 500 hrs.

I also remember that when Denka first brought out LaB6 filaments for TEMs,
they did have some quality control problems, and a few filaments only
lasted 50-100 hours. I think I sent two back. So, if you are doing
everything else right, go back to your supplier - there might be a real
problem with the filament.

Regards,

Larry Stoter






From: C Bower :      paxcb-at-unix.ccc.nottingham.ac.uk
Date: Wed, 18 Sep 1996 13:17:53 +0100 (BST)
Subject: 1D Fourier transforms (fwd)

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Dear All,

I am trying to assess the degree of periodicity and in greyscale images
with vertical stripes, so to this end I am using NIH-image to extract the
pixel values of horizontal lines in an image. I then load these into Excel to
obtain a 1D FFT,the idea being to average over all the lines in the
image.

However I'm not sure how to:

(i) Obtain the spacial frequency of the FFT:- for every pixel brightness
value along the line the result of the FFT is an imaginary number so how
is the amplitude at a particular spacial frequency determined? And indeed
what is the range of spacial frequencies over which the FFT has been
performed?

(ii) Is there any easier way to perform a series of 1D FFT's over each
line of the image and obtain a resulting 'Amplitude vs' spacial frequency
plot' for the image that detects vertical periodicity?

Any comments would be appreciated!

Chris Bower












From: _ _ _ _ _ MARK W. LUND _ _ _ _ _ :      lundm-at-acousb.byu.edu
Date: Wed, 18 Sep 1996 10:45:06 MST/MDT
Subject: RE: LM - Microscope Objective

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I have used the Nikon correction lens for looking at WORM pits
on glass substrates. I would suggest stacking up glass or polycarbonate
sheets until you are within the Nikon's range
best regards
mark
Mark W. Lund, PhD
Director } } Soft X-ray Web page http://www.moxtek.com { {
MOXTEK, Inc. *************************************************
Orem UT 84057 **"Soft x-rays in the 21st Century" conference **
801-225-0930 ** 8-11 January 1997, Midway Utah **
FAX 801-221-1121 ** http://volta.byu.edu/xray/info.html **
lundm-at-xray.byu.edu *************************************************

"Let me commend a great truth to you which has been one of the supports
of my life: 'The Gods send threads for a web begun.' Andrew Carnegie




From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Wed, 18 Sep 1996 11:25:31 -0600
Subject: TEM/easy boats

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Greetings,
And appologies: a few weeks ago in the discussion about sharing
diamond knives, someone commented on a great system for making boats on
glass knives. It was supposed to be much easier that the good old silver
tape method. I lost that post, and can't find it in the "Tips and Tricks"
archive. If the original poster is listening, I would appreciate a reprise,
or if someone else has it, or a suggestion, that would be great too.
Many thanks,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: Warren Straszheim :      wes-at-ameslab.gov
Date: Wed, 18 Sep 1996 13:48:27 -0500
Subject: Re: Digital Image: B/W to B/color

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At 09:51 AM 9/18/96 -0400, you wrote:
} The idea is to use a particular color, rather than white, to represent the
} presence of an element in an EDS dot map. Is there a way to convert a B/W
} digital image to B/color image using either Photoshop or NIH-Image? Thanks
} for any input.
}
} Michael Cinibulk

It may not be easy, but it can be done in PhotoStyler. I suppose PhotoShop
has a similar feature.

It is possible to do an Edit/Fill/Color-Only operation where the chosen
color replaces the gray value. E.g., bright white becomes bright red while
black remains black. However, it is first necessary to convert the image to
a true color (i.e., 24-bit image).

I also was able to convert the white-on-black to color-on-white, but that
took a few more steps which I don't recall off hand. But I could find them
if you need them. I know it involved at least one negative operation.
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: RonMervis-at-aol.com
Date: Wed, 18 Sep 1996 15:24:11 -0400
Subject: sharpening microtome knives - thanks to all

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to all who sent me info regarding knives...thanks much...I received a nice
sample of folks who do this and if anyone would like the names of companies
who perform the service please feel free to contact me....
thanks again.. & bravo for e-mail !!!
Ron Mervis, Ph.D.
NeuroMetrix Research, Inc.
"...assessing the neuroanatomical correlates of neuroplasticity,
neurodegeneration, neurotrauma, ischemia, and cognitive dysfunction...."
RonMervis-at-aol.com
tel: 614-486-6080
fax:614-486-6020




From: GARONEL-at-cliffy.polaroid.com
Date: Wed, 18 Sep 1996 09:47 -0400 (EDT)
Subject: sharpening microtome knives - thanks to all

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We have a JEOL 2000FX which is routinely tested for X-ray's with a
geiger counter by our radiation officer and for the last ten years has
essentially shown no radiation above background. Occassionally there
is a signal in the area between the gun and the condenser lens
(something we have never been able to explain because of the heavy
shielding in the column in that area) but never at a level of concern.
At our last inspection the area around the condenser apertueres showed
up with a level above the min. dose. We found that at large beam
conditions with Pt apertures this was an issue. It was curious that we
"suddenly" had this problem for no explained reason. The vendor came
in and replaced the shielding around the condeser aperture fixture.
This did not decrease the X-ray signal. Our solution has been to
replace the Pt apertures with Mo. The signal is now 10 fold less. Has
anyone else experienced this? Any ideas what's going on?

Thanks, Lynne




From: cinibumk-at-ml.wpafb.af.mil at -SMTPlink
Date: 9/18/96 6:51 AM
Subject: Digital Image: B/W to B/color

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Hi Mike (and all)

Photoshop should do this fine. Bring the image in (probably as a PICT file)
then change to RGB color under the Mode menu. Go to the Select Menu and choose
"Color Range". You can either select Highlights (for the white pixels) or
"Selected Color" which you can then select (by clicking the cursor on a
representative pixel). One problem, if you have light areas on the gray scale
image that are not part of what you want to change to color, you may have to be
selective about how you do this. Once your pixels are selected, pick a
foreground color you want the pixels to become (use the Picker pallette). Then
choose Fill from the Edit menu and select "foreground color". The program will
then fill everything selected with the color you chose.

If these directions are too confusing, let me know off line and I could walk you
through it on the phone.

I imagine NIH Image will also do something like this but I'm not as familiar
with that program.

Cheers,

John Vetrano
_______________________________________________________________________________

The idea is to use a particular color, rather than white, to represent the
presence of an element in an EDS dot map. Is there a way to convert a B/W
digital image to B/color image using either Photoshop or NIH-Image? Thanks
for any input.

Michael Cinibulk
UES, Inc. at
Wright Laboratory
Wright-Patterson AFB, OH

cinibumk-at-ml.wpafb.af.mil




From: rgrappe-at-MMM.COM
Date: Wed, 18 Sep 1996 09:31:36 -0500
Subject: LM - Microscope Objective

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I have a project which requires that I look through .6mm of
polycarbonate with a 100x objective. In the past I have been looking
through 1.2mm of polycarbonate and used a 100x Nikon PC objective with
the correction collar that goes from .9mm to 1.5mm. The microscope I am
using is a Zeiss Axioplan. Does anyone know of a microsope objective
with correction in the .6mm range.

Thanks for your help.

Rod Rappe
Imation Co.
RGRappe-at-mmm.com




From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 18 Sep 1996 14:33:22 -0400 (EDT)
Subject: Re: Polymer analysis

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} I would like suggestions for a method and/or procedure for analyzing the
} diffusion gradient of sodium and chloride ions that are transported into a
} 0.25" thick polymer under cathodic protection (a negative potential of -1.0 V
} vs. saturated calomel). Ion contents most likely will be less than 1% of
} the polymer content (only C, O, H in chains)
}
Dear David,
How much spatial resolution are you looking for, and do you want
the 3-D distribution of the ions, or are these distributions uniform over
the area of the polymer (perpendicular to the direction of diffusion).
If the latter, cut thin cryosections--the resolution will be the thick-
ness of the section--and use something like atomic absorption spectro-
scopy to get the concentration for each section. If there is a non-uni-
form source (e.g., a point on the top surface of the polymer where the
ions diffuse from), cut thin cryosections and use either wavelength-
dispersive x-ray microanalysis or electron energy-loss spectroscopy.
You will be pushing hard at the sensitivity limits for these techniques,
but for a reasonable fraction of 1% ion concentrations, you will prob-
ably get useful results. The spatial resolution will depend on such
factors as beam size, incident electron energy and section thickness.
Having only low-z materials in the matrix will cut down brehmsstrahlung
background, and this is favorable. Good luck.
Yours,
Bill Tivol





From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 18 Sep 1996 14:18:02 -0400 (EDT)
Subject: Re: 1D Fourier transforms (fwd)

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Dear Chris,

} I am trying to assess the degree of periodicity and in greyscale images
} with vertical stripes, so to this end I am using NIH-image to extract the
} pixel values of horizontal lines in an image. I then load these into Excel to
} obtain a 1D FFT,the idea being to average over all the lines in the
} image.
}
} However I'm not sure how to:
}
} (i) Obtain the spacial frequency of the FFT:- for every pixel brightness
} value along the line the result of the FFT is an imaginary number so how
} is the amplitude at a particular spacial frequency determined? And indeed
} what is the range of spacial frequencies over which the FFT has been
} performed?
}
If you have 2N pixels along each line, you can get spatial frequen-
cies from 0 to N. The FFT in particular will give values for this frequen-
cy range. The algorithm for the FFT produces a complex amplitude for each
of the spatial frequencies, and the squares of the moduli constitute the
power spectrum. The FFT does not give a value "for every pixel brightness
value along the line"; the "amplitude at a particular spatial frequency"
depends on the brightness values at all the pixels and how these values
change from one pixel to the next.

} (ii) Is there any easier way to perform a series of 1D FFT's over each
} line of the image and obtain a resulting 'Amplitude vs' spacial frequency
} plot' for the image that detects vertical periodicity?
}
Peaks in the power spectrum will correspond to periodicities in
each line. You should be aware that these need not be vertical features.
If there are features at, say, 45 deg, each line will have a peak at the
same spatial frequency; however, the phase of the amplitude will vary
from line to line.

| / / / / / / / / / / note periodicity
y-axis | / / / / / / / / / / of 3 spaces
| / / / / / / / / / / in each line.
____________________________________
x-axis

In order to get strictly the vertical features, you
should perform a 2-D Fourier transform and look at the horizontal compon-
ents. That is, if x is horizontal and y is vertical, a vertical feature
does not vary with y, so the Fourier transform--which has components de-
pending on (hx/2pi + ky/2pi) will be represented by amplitudes for which
k = 0.
Yours,
Bill Tivol




From: (Marek Malecki, M.D., Ph.D.) :      malecki-at-MACC.WISC.EDU
Date: Wed, 18 Sep 1996 13:46:56 -0500
Subject: Symposium on Energy-Filtered Transmission Electron Microscopy

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Microscopists:
The Campus Committee on Microscopic Imaging and Analysis
at the University of Wisconsin-Madison is pleased to announce a half day
symposium on Energy-Filtered Transmission Electron Microscopy.
The symposium will be held on October 3, 1996 room 140 Bardeen Hall
1215 Linden Drive, Madison. Two world renowned scientist in this field
will give lectures containing introductory information and demonstrating
how this emerging technology may be useful for a wide range of applications in
the biological and physical sciences. There will be a short demonstration
of the new LEO EM912 Omega Filter TEM in room 146 before the lectures.
Investigators willing to present and discuss their result can contact
organizers
to schedule short, contributed presentations. For parking arrangements
please contact organizers. Admission is free.

The schedule is as follows:

The 3rd of October 1996.

Exhibition in room 146 Bardeen Hall
1 PM Demonstration of the EFTEM 912 Omega.

Lectures in room 140 Bardeen Hall
2 PM Energy filtered electron microscopy. Perspectives into the past
and future. Prof. Peter
Ottensmeyer, Ontario Cancer Institute,
Toronto, Canada.
3 PM Performance and applications of integrated EFTEM. Dr. Wolfgang
Probst, LEO Electron Microscopy,
Oberkochen, Germany.
4 PM Contributed Lectures.
5 PM Reception.

If you would like any further information, please contact
Marek Malecki by email: malecki-at-macc.wisc.edu, Grayson Scott
by email: glscott-at-facstaff.wisc.edu or phone 608 262-2993, or
Tom Kelly by email: tfkelly-at-engr.wisc.edu or phone: 608 263-1073.

Marek Malecki, Chairman.
Energy Filtered Transmission Electron Microscopy Committee.









From: ebs-at-ebsciences.com
Date: Wed, 18 Sep 1996 10:08:35 -0500
Subject: Re: lab6 filaments

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Dear Raj and colleagues-

Energy Beam Sciences is the North American distributor for Denka LaB6
cathodes. I want to take this opportunity to clear up a common
misperception about these filaments. In the Denka Model 3 (M-3) design, the
LaB6 crystal is placed into a tantalum "cup" and held to the tungsten wires
by a ceramic binding. There are two different binding materials used in
this process, and they cool at different rates. The interfaces between the
two binders exhibit a characteristic vertical "crack." These cracks are
*not* manufacturing defects, and are not stress points or the cause of
premature filament burn-out or other failure.

In a good vacuum, the Model 3 cathode lifetime varies between 500 hours
(minimum) and well over 1000 hours. Any cathode which does not give at
least 500 hours life should be returned to us for failure analysis. On the
rare occasions where premature burnout can be attributed to a manufacturing
defect, the returned cathode would be cheerfully replaced.

I do not want to comment on competitive products, except to say that, to my
knowledge, none of them guarantee a minimum lifetime (in hours).

Steven E. Slap, Vice-President


********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: Kirk Rogers :      rogers-at-kcgl1.eng.ohio-state.edu
Date: Wed, 18 Sep 1996 13:05:22 +0000
Subject: Re: angio digitizing board

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microscopy {Microscopy-at-sparc5.microscopy.com}
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dominic

} I would like to built a digital archive (CD ROM) in my angiographic
} cathlab.
} The outgoing video signal is a grayscale S-Video PAL.
} What PCI based digitizing board do you suggest?
} Is there anybody who faced with this argument.

Some time ago on the microscopy listserver {Microscopy-at-MSA.Microscopy.Com}
someone wrote the following:

The Scion LG-3 frame grabber is about $900 and does an excellent job
digitizing images with the NIH Image software. One caveat - make sure you
have a full-size (12") PCI or NuBus slot in your PowerMac (the 6100, for
example, has a 7" slot). Be sure to specify video format (US video =
NTSC/RS-170, European video = PAL/CCIR).

The LG-3 framegrabber is limited to about 20 frames/sec on a decent
Powermac using NIH Image. Scion may also have other framegrabbers that
are of interest.

Scion Corporation
152 West Patrick St.
Frederick, MD 21701 USA

phone: 301-695-7870
fax: 301-695-0035


-Kirk
___________________________________________________
This message was created and sent using the Cyberdog Mail System
___________________________________________________
Kirk Rogers E: rogers-at-er6.eng.ohio-state.edu
Office: (614) 688-4067 FAX: (614) 292-1537
Materials Science and Engineering The Ohio State University
477 Watts Hall, 2041 College Ave. Columbus, OH 43210








From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Thu, 19 Sep 1996 16:01:35 +1000 (EST)
Subject: Re: Digital Image: B/W to B/color

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In photoshop, load your image, go to MODE, select INDEXED COLOR . The
program will change the image to indexed mode.
Go to COLOR TABLE and choose CUSTOM. You will see a matrix of 256 color
patches. Click on the top left patch, which represents white. You are
presented with a color square. Pick red (if you like red!) or in the RGB
boxes, set R =255 B=0, G=0. Click OK. Top left square should turn red.
Click OK on table menu. All white areas should turn red. Non X-ray dots
that are red can be edited. Of course if you know in advance you will make
sure no detail in the underlying image goes into white.

Mel Dickson





From: Gordon Watt :      gwatt-at-brookes.ac.uk
Date: Thu, 19 Sep 1996 09:31:28 -0700
Subject: B+W images to colour

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Message-ID: {32417560.20C6-at-brookes.ac.uk}

Although we use the colour lookup tables supplied with our Oxford
Instruments EXL EDS package, I've been playing with Adobe Photoshop to
try and improve some of the LUTs (or palettes as they're referred to in
Photoshop and most other packages).

The method I use for adding colour is to convert the 512x512x16bit (but
only 8 bits contain data) image to "indexed colour mode" and then apply
palettes as required. The advantage of this method is that you can
customise the palette easily, save palettes from images you like (I've
converted the EXL lookup tables to Windows palettes in this way) and it
works really well and is very very easy.

Although Photoshop is expensive, a "lite" version (Photoshop LE) comes
bundled with many scanners so there may be a copy available to you?

If anybody else out there knows of any sources for palettes files I'd
like to know - I'm getting a bit bored with Thermal colour schemes!

Gordon




From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Thu, 19 Sep 1996 09:30:58 +0100
Subject: Re: Digital Image: B/W to B/color

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} The idea is to use a particular color, rather than white, to represent the
} presence of an element in an EDS dot map. Is there a way to convert a B/W
} digital image to B/color image using either Photoshop or NIH-Image? Thanks
} for any input.
}
} Michael Cinibulk
} UES, Inc. at
} Wright Laboratory
} Wright-Patterson AFB, OH
}
} cinibumk-at-ml.wpafb.af.mil

You can, in principle, do it in NIH-Image but it is tricky - at least, I
can't work out a straightforward way to do it. Also, NIH-Image doesn't like
images that aren't either 8 or 16 bits deep, so you would first have to may
sure your B/W x-ray dot map was converted to 8-bits.

I don't know but guess that it can be done more easily in Photoshop.

However, my preference would be Graphic Converter (I'm assuming you're Mac
based in all this). I would say anyone working with images should have this
application (I have no interest in the programme, or anyone connected with
it). Simply, it allows images to be switched between all sorts of formats,
Mac, PC, and others. There are something like 50 formats it can open and
about 35 it can save. There are a number of useful features but one is
fairly comprehensive LUT editing, including sorting, minimising and direct
editing of any value. I often use it for reducing the size of image files -
read in a 24bit image which only really has useful information in a few
levels, select the levels I want and save as a 4 bit image.

Regards,
Larry






From: Probing & Structure :      p&s-at-ultra.net.au
Date: Thu, 19 Sep 1996 18:59:11 +1000
Subject: Re: Digital Image: B/W to B/color

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Lynne wrote on the 18 Sept.96:
We have a JEOL 2000FX which is routinely tested for X-ray's with a
geiger counter by our radiation officer and for the last ten years has
essentially shown no radiation above background. Occassionally there
is a signal in the area between the gun and the condenser lens
(something we have never been able to explain because of the heavy
shielding in the column in that area) but never at a level of concern.
At our last inspection the area around the condenser apertueres showed
up with a level above the min. dose. We found that at large beam
conditions with Pt apertures this was an issue. It was curious that we
"suddenly" had this problem for no explained reason. The vendor came
in and replaced the shielding around the condeser aperture fixture.
This did not decrease the X-ray signal. Our solution has been to
replace the Pt apertures with Mo. The signal is now 10 fold less. Has
anyone else experienced this? Any ideas what's going on?

Thanks, Lynne
**************************************
It's obvious (if you know)Lynne:
Mo is element 42 and it's highest energy X-rays are 19999.5 eV. Pt
is element 78 and it's highest energy X-rays are 78394.8 eV. The Pt X-rays
are much more energetic and will penetrate more material and in turn
generate more X-rays from the materials along the way.
By definition, it is impossible to generate X-rays of greater energy
than the accellerating voltage used. Maximum fluorescence requires about
1.8x greater voltage than the X-rays generated. Your FX2000, at full voltage
has plenty of "boot" to generate at lot of powerful Pt K alpha X-rays.
Cheers
Jim Darley

Probing & Structure
} (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia
}
} Phone +61 77 740 370 Fax: +61 77 892 313
} A great microscopy site http://www.ultra.net.au/~pns/





From: Walter A. Mannheimer :      wamann-at-metalmat.ufrj.br
Date: Thu, 19 Sep 1996 08:43:49 EST3EDT
Subject: SEM explosion

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Fellow microscopists, thanks to all who contributed so far to our
dilema. We are still awaiting a position from the manufacturer
service.
I will be away from the office abroad, and will post our conclusions
when I return. Regards to all
Prof.Walter A.Mannheimer
Metallurgy and Materials Engineering
Federal University of Rio de Janeiro
POBox 68505 21945 Rio de Janeiro, Brazil
Voice +5521 280-7443 (Dept.office) +5521 590-0579 (direct)
Fax +5521 290-6626 Email: wamann-at-metalmat.ufrj.br




From: John Best :      jbest-at-vicon.net
Date: Thu, 19 Sep 1996 09:15:04 -0700
Subject: 3D sample request

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Microscopy-at-Sparc5.Microscopy.Com (MICROSCOPY MSA list messages)
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Tobias

it sounds like a reply I made (unless anyone else did, when I wasn't
looking).

The glass knife boats were made by LKB (I don't know who markets their stuff
in the States). They are 'disposable' plastic water troughs called LKB
TRUFFS and can be attached to a slightly warmed glass knife, using molten
dental wax. LKB also manufactured a special hot-plate/wax dispenser called
an LKB 2208 multiplate which makes the process much easier (although any
suitable hot-plate at about 60-70 deg. C should do). All you do is keep a
reservoir of wax hot then warm up knives (not the very cutting edge) for a
few seconds, put a thin layer of molten wax onto the edge of the TRUF and
attach it to the knife. It's usually advisable to go around the outside of
the TRUF with a warmed spatula and a bit more wax to make sure of the seal.

The big advantage is that with a little practice you can easily produce
large rigid water baths in seconds. The disadvantage is that they cost about
0.15 to 0.2 UK pound each but even a thousand would be cheaper than ay
diamond

If you can't find a cheap LKB supplier you could try AGAR SCIENTIFIC,
BALZERS, BIO-RAD, SPI because some of these probably still sell the
hot-plates.

Malcolm Haswell
University of Sunderland
U.K.

DISCLAIMER: I have no personal interest in any of the above companies.
----------

A Good Day to All,

Would anyone be so kind as to send me a sample of a diatomaceous sand?
I'm playing around with 3D image capture from the SEM, have seen some
outstanding 3D diatomes, and would like to digitize same.

Does anyone have any comments about color purity on various SVGA monitors
, quality of 3D viewing glasses, or techniques in general for capturing
3D images that jump right off the screen?

Regards,
John.

--
John W. Best ELMDAS Co. Email: jbest-at-vicon.net
P.O. Box 355, Alexandria, PA, USA 16611
Voice: 814-669-4474
WWW: http://www.vicon.net/~jbest





From: scott.wight-at-nist.gov (Scott Wight)
Date: Thu, 19 Sep 1996 10:02:26 -0500
Subject: Re: Digital Image: B/W to B/color

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Actually is is simple in NIH Image, load your grey scaled image then select
"density slice" from the "options" menu. Use the lut tool to slide the top
and bottom of the red up and down until your white pixels are red and all
else is black.
Scott Wight

} } The idea is to use a particular color, rather than white, to represent the
} } presence of an element in an EDS dot map. Is there a way to convert a B/W
} } digital image to B/color image using either Photoshop or NIH-Image? Thanks
} } for any input.
} }
} } Michael Cinibulk
} } UES, Inc. at
} } Wright Laboratory
} } Wright-Patterson AFB, OH
} }
} } cinibumk-at-ml.wpafb.af.mil
}
} You can, in principle, do it in NIH-Image but it is tricky - at least, I
} can't work out a straightforward way to do it. Also, NIH-Image doesn't like
} images that aren't either 8 or 16 bits deep, so you would first have to may
} sure your B/W x-ray dot map was converted to 8-bits.
}
} I don't know but guess that it can be done more easily in Photoshop.
}
} However, my preference would be Graphic Converter (I'm assuming you're Mac
} based in all this). I would say anyone working with images should have this
} application (I have no interest in the programme, or anyone connected with
} it). Simply, it allows images to be switched between all sorts of formats,
} Mac, PC, and others. There are something like 50 formats it can open and
} about 35 it can save. There are a number of useful features but one is
} fairly comprehensive LUT editing, including sorting, minimising and direct
} editing of any value. I often use it for reducing the size of image files -
} read in a 24bit image which only really has useful information in a few
} levels, select the levels I want and save as a 4 bit image.
}
} Regards,
} Larry

Scott Wight e-mail: SCOTT.WIGHT-at-NIST.GOV
NIST - Microanalysis Group W voice: 301-975-3949
Bld 222, Rm A113 | fax: 301-216-1134 or 301-417-1321
Gaithersburg, MD 20899 \|/ disclaimer:
Any opinion expressed is my own and does not represent those of my employer.






From: wise-at-vaxa.cis.uwosh.edu
Date: Thu, 19 Sep 1996 10:36:07 +0000
Subject: RE: TEM/easy boats

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} The glass knife boats were made by LKB (I don't know who markets their stuff
} in the States). They are 'disposable' plastic water troughs called LKB. The
} disadvantage is that they cost about 0.15 to 0.2 UK pound each but even a
} thousand would be cheaper than a diamond


We reuse our Trufs. We scrape off the old wax, rinse them in alcohol, and
throw them back in the drawer. Occassionally we get "scuzzy" water in a
re-used Truf (probably from a dirty Truf) but the problem is not very
frequent or serious. We just throw that knife away.

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-uwosh.edu






From: kelloes-at-emlab.cb.uga.edu
Date: Thu, 19 Sep 1996 12:50:54 +0000
Subject: Hummer Sputter Coaters

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We have two Hummer Sputter Coaters , which need repaired. We are
offering these for sale. The cost would only be paying for the
shipping and handling. They come with gold/palladium targets; however,
other targets are available . If anyone is interested please let me
know. Thanks Cathy Kelloes




From: Steve Pfeiffer :      103455.3701-at-CompuServe.COM
Date: 19 Sep 96 12:42:15 EDT
Subject: Nominations please

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AFM _ STM


Attention All Electrochemists/ Scanning Probe Microscopists.


Molecular Imaging is soliciting nominations for the "Young Electrochmical
Scanning Probe Microscopist of 1996". The winner of this award will
receive a PicoSPM from Molecular Imaging configured to the winner's
specifications.


A ballot for nominations may be found on Molecular Imaging's Web page
{www.molec.com} . Please, only one nomination per person. The winner
will be selected by peer review using a panel of renowned
electrochemical scanning probe microscopists. Complete
rules are posted with the ballot.


Thank you

Steve Pfeiffer
Director of Sales and Marketing
Molecular Imaging
steve-at-molec.com





From: Beverly E Maleeff
Date: 19 Sep 96 12:42:34 EDT
Subject: Resolution in processed images

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Message-Id: {9609191928.AA1613-at-pho903.sbphrd.com}
To: microscopy {microscopy-at-sparc5.microscopy.com}
{Beverly_E_Maleeff-at-sbphrd.com}

Question-- can you measure a loss in resolution after processing a digitized
image? For example, if we have an image of a single virus particle and perform
a 2D-FFT on all or part of the image, can a measurement of resolution
difference (loss) be made, and if so, how? It seems logical just to do a
point-to-point measurement before and after processing, but are there any other
ways that are used to determine resolution differences?

TIA-
Bev Maleeff

SmithKline Beecham Pharmaceuticals
Toxicology-US, UE 0462
709 Swedeland Road
King of Prussia, PA 19406
610/270-7987
610/270-7202 fax
Beverly_E_Maleeff-at-sbphrd.com





From: rnessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Thu, 19 Sep 1996 15:17:43 -0500 (CDT)
Subject: RE: TEM/easy boats

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Microscopy-at-Sparc5.Microscopy.Com

On Thu, 19 Sep 1996 wise-at-vaxa.cis.uwosh.edu wrote:

}
}
} We reuse our Trufs.
}
} Bob
}
}
} Robert R. Wise, PhD

Instead of wax, we buy cheap finger nail polish that the local
Osco drugstore discounts because it wouldn't sell (ugly colors, etc.).
You don't have to have the equipment to keep molten wax handy. Also, when
reusing the Truf, rather large gaps can be filled with polish.

Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu





From: Christophe Roos :      ROOS-at-operoni.helsinki.fi
Date: Thu, 19 Sep 1996 18:20:31 EET DST
Subject: LUT / palette editor?

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Dear reader,

I am looking for a palette / LUT editor that might make the editing a
bit easier than what can be done with a standard spreadsheet program.

The programs I am using are among other PaintShop pro and ImageTool
and their LUT-files are plain ASCII files with one column for each of
the Red/Green/Blue colors and one line for each grey value (0-255).

Thank you for your attention.

ChR

_________________________________________________________________________
Christophe Roos Dr.Sc., doc. | Institute of Biotechnology
| & Dept. of Biosciences
Phone: +358 0 7085 9367 | Division of Genetics
Fax: +358 0 7085 9366 | P.O.Box 56, Viksbagen 9
E-mail: Christophe.Roos-at-Helsinki.FI | FIN-00014 Univ. of Helsinki
X-400: /G=Christophe/S=Roos/O=Helsinki/ADMD=fumail/C=Fi Finland
{A HREF="http://www.helsinki.fi/~roos/index.html"} WWW Home Page: Roos {/A}
-------------------------------------------------------------------------





From: Gejing Li :      gjinli-at-umich.edu
Date: Thu, 19 Sep 1996 18:31:19 -0400 (EDT)
Subject: unsubscribe

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please remove my name from your list. Thanks






From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 19 Sep 1996 13:21:59 -0400 (EDT)
Subject: Re: X-rays from JEOL 2000FX

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} We have a JEOL 2000FX which is routinely tested for X-ray's with a
} geiger counter by our radiation officer and for the last ten years has
} essentially shown no radiation above background. Occassionally there
} is a signal in the area between the gun and the condenser lens
} (something we have never been able to explain because of the heavy
} shielding in the column in that area) but never at a level of concern.

You should ascertain whether your shielding might not have small
spaces between lead pieces. Sometimes these spaces allow beams of radiation
to pass through. Electrons can scatter several times at large angles from
lead atoms and thereby go around corners etc., so if there are possible
pathways around the shielding, this could account for the signal.

} At our last inspection the area around the condenser apertueres showed
} up with a level above the min. dose. We found that at large beam
} conditions with Pt apertures this was an issue. It was curious that we
} "suddenly" had this problem for no explained reason. The vendor came
} in and replaced the shielding around the condeser aperture fixture.
} This did not decrease the X-ray signal.

Slight changes in beam conditions can possibly illuminate high-Z
material in the column, producing a sudden appearance of x-rays for no
explained reason. The changes in the shielding would not be expected to
fix things in that case.

} Our solution has been to
} replace the Pt apertures with Mo. The signal is now 10 fold less.

Brehmsstrahlung production goes as Z^2, which gives a factor of ~4.
The remaining difference could arise from differences in geometry of the
two types of aperture, but that may be stretching things. I don't know
how the effects of other processes (e.g., scattering of electrons from the
aperture and subsequent x-ray production) vary with Z, but any relevant
process must obviously involve interaction with the aperture. We use an
aluminum aperture at the top of our lens column (it doesn't define the
beam, so the oxide coating the aluminum is no bother) and we have tried
berylium apertures in the second condenser lens. These give a ragged-
looking beam, but seem to be OK otherwise. We have never seen any prob-
lems with x-rays. We have monitors permanently mounted to the column in
addition to inspections by the safety office.
Yours,
Bill Tivol




From: gjli-at-asu.edu
Date: Thu, 19 Sep 1996 15:33:43 -0700 (MST)
Subject: subscribe

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Please put my email address to your mailing list. Thanks.




From: masur-at-Inka.MSSM.EDU (Dr. Sandra Masur)
Date: Thu, 19 Sep 1996 19:01:21 -0400 (EDT)
Subject: subscribe

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unsubscribe

Best regards,

Sandra K. Masur, Ph.D. masur-at-inka.mssm.edu
Box 1183
Depts of Ophthalmology,and
Cell Biology/Anatomy
Mount Sinai School of Medicine phone: 212-241-0089 or 6544
1 Gustave Levy Place
Annenberg Building 22-20
New York NY 10029-6574 fax: 212-289-5945






From: (Marek Malecki, M.D., Ph.D.) :      malecki-at-vms2.macc.wisc.edu
Date: Thu, 19 Sep 1996 20:01:08 -0500
Subject: WANTED - used inverted light microscope.

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Microscopists,
We would like to buy for less than $3000.- an inverted light microscope
(used or demo model) equipped with a port for a camera (C-mount). It will
be used for routine cell culture work. Ph-c optics with long working
distances preferred. 160 tubus length also acceptable.
Marek Malecki.
Email: malecki-at-macc.wisc.edu
Phone: 6082638481





From: (Marek Malecki, M.D., Ph.D.) :      malecki-at-vms2.macc.wisc.edu
Date: Thu, 19 Sep 1996 20:01:08 -0500
Subject: WANTED - used inverted light microscope.

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Microscopists,
We would like to buy for less than $3000.- an inverted light microscope
(used or demo model) equipped with a port for a camera (C-mount). It will
be used for routine cell culture work. Ph-c optics with long working
distances preferred. 160 tubus length also acceptable.
Marek Malecki.
Email: malecki-at-macc.wisc.edu
Phone: 6082638481





From: masur-at-Inka.MSSM.EDU (Dr. Sandra Masur)
Date: Thu, 19 Sep 1996 20:42:33 -0400 (EDT)
Subject: unsubscribe

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Best regards,

Sandra K. Masur, Ph.D. masur-at-inka.mssm.edu
Box 1183
Depts of Ophthalmology,and
Cell Biology/Anatomy
Mount Sinai School of Medicine phone: 212-241-0089 or 6544
1 Gustave Levy Place
Annenberg Building 22-20
New York NY 10029-6574 fax: 212-289-5945






From: Al :      al-at-execpc.com (by way of Nestor J. Zaluzec)
Date: Thu, 19 Sep 1996 18:27:05 -0500
Subject: Microscope allignment

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Colleagues

Can anyone in our Wisconsin area help this guy?
Please reply direct to "al-at-execpc.com" he is not
a member of the Listserver subscribers group

Nestor
Your Friendly Neighborhood SysOp
===========================================================

Hi. I recently aquired a Nikon binocular microscope that needs
allignment. I'm having trouble finding services in the Milwaukee area.
Would any of your members know of an optics lab that can do this for me?
Thanks! Sincerely, Al (al-at-execpc.com)






From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Fri, 20 Sep 1996 09:28:26 +1000 (EST)
Subject: Easy boats on glass knives

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LKB USED to sell, and Baltec still sell, metal (brass?) boats which you can
wax onto glass. The nice thing being that after the first waxing, which
involves a bit of thrashing around with dental wax and a hot micro spatula,
the baths crack cleanly off the glass, taking the wax with them.

Then you need only clip the bath back on a fresh knife and warm the bottom
where the wax is over a small (eg spirit lamp) flame. The wax melts and
capillarity makes it run into and seal all the cracks twixt boat and knife.
A little dabble with a warm spatula in the bottom at the back and you have a
guaranteed leak proof boat. The boats cost $$$ but they last .

Mel Dickson





From: Jeff Duerr :      JDUERR-at-zoogate.zoo.hawaii.edu
Date: Thu, 19 Sep 1996 16:30:31 -1000
Subject: % H2O in cells?

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Hello out there,

I have question I was hoping somebody could answer. Does anybody
know what average percentage of a cell's volume is aqueous versus
solid (organelles, ect.)??? I'm trying to estimate intracellular K
concentration in lobster hepatopancreas E-cells.

Thanks and aloha,
Jeff.

Jeff Duerr University of Hawaii - Zoology
jduerr-at-zoogate.zoo.hawaii.edu 808-956-4718
jduerr-at-uhunix.uhcc.hawaii.edu 808-956-4723
WWW access coming soon.




From: ROBIN CROSS :      EURC-at-giraffe.ru.ac.za
Date: Fri, 20 Sep 1996 10:41:02 GMT+0200
Subject: JEOL 733 upgrade

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Message-ID: {199609192317.SAA00434-at-IndyNet.indy.net}
To: C Bower {paxcb-at-unix.ccc.nottingham.ac.uk} ,
Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

Just to add to Malcolm's reply: LKB was swallowed by Leica and I expect that
they provide those glass knife boats as do EMS and Pelco in the USA and we
(P&S) do in Australasia. The trufs can be re-used after very minor cleaning.
Yet many people still use (prefer) "silver tape".

Jim Darley
A great microscopy site http://www.ultra.net.au/~pns/
*********************************************************

Tobias

it sounds like a reply I made (unless anyone else did, when I wasn't
looking).

The glass knife boats were made by LKB (I don't know who markets their stuff
in the States). They are 'disposable' plastic water troughs called LKB
TRUFFS and can be attached to a slightly warmed glass knife, using molten
dental wax. LKB also manufactured a special hot-plate/wax dispenser called
an LKB 2208 multiplate which makes the process much easier (although any
suitable hot-plate at about 60-70 deg. C should do). All you do is keep a
reservoir of wax hot then warm up knives (not the very cutting edge) for a
few seconds, put a thin layer of molten wax onto the edge of the TRUF and
attach it to the knife. It's usually advisable to go around the outside of
the TRUF with a warmed spatula and a bit more wax to make sure of the seal.

The big advantage is that with a little practice you can easily produce
large rigid water baths in seconds. The disadvantage is that they cost about
0.15 to 0.2 UK pound each but even a thousand would be cheaper than ay
diamond

If you can't find a cheap LKB supplier you could try AGAR SCIENTIFIC,
BALZERS, BIO-RAD, SPI because some of these probably still sell the
hot-plates.

Malcolm Haswell
University of Sunderland
U.K.

DISCLAIMER: I have no personal interest in any of the above companies.
----------

I received several interesting replies to my question on JEOL
733 upgrades. Anyone wishing to receive a summary of these
is welcome to email me directly.


Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)




From: ROBIN CROSS :      EURC-at-giraffe.ru.ac.za
Date: Fri, 20 Sep 1996 10:39:13 GMT+0200
Subject: glass knife boats

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For several years we have used only the plastic LKB "trufs".
However, having read Mel Dickson's message about the brass
boats I realised that there may be people now looking for these.
We had several at one time so I am sure if there is a need I
could find them.


Robin Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za (tel: +27 461 318168 - fax: +27 461 24377)




From: John Best :      jbest-at-vicon.net
Date: Fri, 20 Sep 1996 09:13:21 -0700
Subject: Swimming Pool Sand

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Message-ID: {3242C2A1.568D-at-vicon.net}

Many thanks to all who suggested trying swimming pool sand as a source
for diatoms. It's even the end of the swimming season here in
Pennsylvania, so I'm likely to get it cheap!

Regards, and have a nice weekend.
John

--
John W. Best ELMDAS Co. Email: jbest-at-vicon.net
P.O. Box 355, Alexandria, PA, USA 16611
Voice: 814-669-4474
WWW: http://www.vicon.net/~jbest





From: Vincent Tsai :      vincent.tsai-at-nist.gov
Date: Fri, 20 Sep 1996 09:53:35 -0400
Subject: "subscribe"

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From: Maite.Caldes-at-cnrs-imn.fr
Date: Fri, 20 Sep 1996 16:22:19 +0200
Subject: subscribe

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From: Peters-at-BSAC.UCHC.EDU (Klaus-Ruediger Peters)
Date: Fri, 20 Sep 1996 10:09:14 -0500
Subject: Re: Resolution in processed images

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} Question-- can you measure a loss in resolution after processing a digitized
} image? ... It seems logical just to do a
} point-to-point measurement before and after processing, but are there any
} other
} ways that are used to determine resolution differences?
*********

Hi Beverly,

Yes, that's easy if you know what where to measure. Often, image components
do not have sufficient contrast to be accurately measured, have varying
intensity background or are obscured by noise.

We do such measurements for image processing (conventional image
processing, and various image compression algorithms) routinely by
quantitative segmentation before and after processing of the contrast
ranges of image components in question, i.e., your virus particles. We use
contrast segmentation by differential hysteresis processing (DHP) that
generates output images of 8-bit with continuous intensity distribution.

Since DHP does not change any spatial image properties you may use it
before your processing to more easily access a component's property and
after processing for their measurement, i.e.,, full width at half maximum,
max. extension above noise, gravity center of particles, area loss of
components etc. Loss in contrast in proportion to raw data is available if
you can include a reference component that is unaffected by the processing,
This procedure is quantitative and provides really useful inside into the
processing characteristics of the analyzed procedure in regard to spatial
resolution and the contrast resolution.

We used this approach also for R&D by quantitatively analyzing image
reconstruction approaches for PET, performances of amplifiers, transducers
of stylus imaging systems etc.

Best regards Klaus






******************************************************************************
* Klaus-Ruediger Peters, Ph.D. :Molecular Imaging Laboratory *
* Director, Molecular Imaging Laboratgory : http://panda.uchc.edu/ *
* Biomolecular Structure Analysis Center : htklaus/index.html *
* University of Connecticut Health Center : *
* 263 Farmington Ave. :F r e e Access to Differential *
* Farmington, CT 06030-2017; U.S.A :Contrast Software at *
* e-Mail: Peters-at-BSAC.UCHC.EDU : http://panda.uchc.edu/ *
* Tel: (860) 679-3977; Fax: (860) 679-1989 : htklaus/DHP-Img.html#Software*
******************************************************************************







From: micro-at-unix.midplains.net (Al Kutchera)
Date: Fri, 20 Sep 1996 13:43:55 -0600
Subject: LM-Need U.S. source Hyrax or Naphrax Mounting Media

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I would appreciate info on a U.S. source for Hyrax or Naphrax Mounting
Media or other high refractive index media for use in mounting Diatoms.
Thanks in advance for your help.

micro-at-unix.midplains.net(Al Kutchera/Midwest MicroTech)






From: tong-at-cebaf.gov (Tong Wang)
Date: Fri, 20 Sep 1996 15:12:49 -0400
Subject: install a Field Emission scanning probe in SEM

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I am considering to install a high voltage Field Emission microtip in an
Amray 1830 SEM chamber equipped with EDX in order to investigate the FE on
niobium sample. The highest voltage will be ~30Kv, and the tip-sample gap
would be tens of micron. Will the vacuum of ~1E-5 --} 1E-6 Torr in the
chamber be a problem of this work? Such as the unstable emission possibly
caused by adsorbates or the residual gas plasma would help to process away
the Field Emitter during the scanning? And anyone has experience in
upgrading the SEM vacuum?
I appreciate your information.

Tong







From: humen001-at-metvax.metro.msus.edu (John Humenansky)
Date: Fri, 20 Sep 1996 18:00:36 -0500
Subject: NanoLab 7 SEM

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Would appreciate any information regarding the NanoLab 7 SEM. Would be
interested in determining if there are some of these out there in use or
available for parts. Also would like to know the resolution, I'm guessing
100-250=C5.

Thanks in advance for any information, and you can reply off line.

John Humenansky






From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Sat, 21 Sep 1996 08:42:17 -0300 (EST)
Subject: confocal microscopy in France

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I would like to communicate with french researchers that work with
confocal microscopy. Could anyone help me?

Thank you
_____________________________________________________________________________
Francisco Javier Hernandez Blazquez * Av. Prof. Lineu Prestes, 1524
Departamento de Histologia e Embriologia * 05508-900 Sao Paulo
Instituto de Ciencias Biomedicas * e-mail fjhblazq-at-spider.usp.br
Universidade de Sao Paulo * fjhblasq-at-biomed.icb2.usp.b
__________Brasil_____________________________________________________________






From: emerald-at-chem.psu.edu
Date: Sun, 22 Sep 1996 02:59:28 -0400
Subject: 1st Laue zone in CBED

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I am interested in finding out about getting unit cell dimension
information in the z direction. I have some patterns and have not been
successful due to limited tilt to index multiple zone axis patterns. In
CBD I do get a nice pattern at the K intersection with the rings for the
first and second laue zones. I am looking for references/advice on how I
may index the pattern based upon this information. Specifically how do I
use the Laue ring???
Thank you in advance for your advice.
Dave






From: lag-at-pegasus.cc.ucf.edu (Lucille A. Giannuzzi)
Date: Sun, 22 Sep 1996 18:13:13 -0400
Subject: characterization centers

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I am in the process of setting up a university-wide user facility that
includes TEM, SEM, as well as RBS, SIMS, microprobe, and Auger. I would
like to know from those who run, or are familiar with centers, the
following types of things:

Does your center support itself (or make money)?

What percentage of the operating budget does the university contribute to?

How much of the budget is run on user fees?

What percentage of your equipment is on service contracts?

Are full-time or part-time technicians (students, post-docs?) used instead
of or in addition to service contracts?

Any other information would be helpful.

Thank you,
Lucille Giannuzzi



*************************************************************************
Lucille A. Giannuzzi, Ph.D.
Assistant Professor

Dept. of Mechanical, Materials, and Aerospace Eng.

University of Central Florida phone (407) 823-5770
PO Box 162450 fax (407) 823-0208
4000 Central Florida Blvd. email lag-at-pegasus.cc.ucf.edu
Orlando, FL 32816-2450 USA
*************************************************************************







From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Mon, 23 Sep 1996 09:48:44 +0100
Subject: Re: 1st Laue zone in CBED

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} I am interested in finding out about getting unit cell dimension
} information in the z direction. I have some patterns and have not been
} successful due to limited tilt to index multiple zone axis patterns. In
} CBD I do get a nice pattern at the K intersection with the rings for the
} first and second laue zones. I am looking for references/advice on how I
} may index the pattern based upon this information. Specifically how do I
} use the Laue ring???
} Thank you in advance for your advice.
} Dave

A number of books on electron diffraction cover this method - try, for
example, 'Practical Analytical Electron Microscopy in Materials Science' by
David Williams, ISBN 0-9612934-0-3, or 'Introduction to Analytical Electron
Microscopy' eds Hren, Goldstein and Joy, pub Plenum Press, New York.

Basically, the equations are:

G1 = square root(2KH), for 1st HOLZ ring

G2 = 2 x square root(KH), for 2nd HOLZ ring

and so on.

(If you do a standard Ewald sphere construction, you can easily work these
out yourself)

where Gn is radius of nth HOLZ ring, K is reciprocal wavelength and H is
lattice spacing perpendicular to plane of diffraction pattern.

Knowing this value, H, and the UVW beam direction, you can then use
standard crystallographic equations to work back to the basic a, b, c
lattice paramenters.

A couple of points to check:

1. The value you get will be no more accurate than any other electron
diffraction method, and possibly worse.

2. The first VISIBLE HOLZ ring may not actually be the first order Laue
zone ring. The FOLZ may be kinematically forbidden (as may be others), so
if you seem to be getting some unexpected values, try sticking in an extra
root(2).

3. There may be distortions in the electron optics such that for large
spacings in the diffraction pattern (as in this case, where you are
measuring the radius of the 1st HOLZ), the camera length is significantly
different from that near the centre of the pattern. That is, to stand a
reasonable chance of getting a good measurement, you need to calibrate the
whole diffraction plane with a known specimen.

4. Often, a HOLZ will spread over several reciprocal lattice spacings,
making measurement of G (the radius of the ring) difficult. If you can, try
to relate a sharp, bright line in the 1st HOLZ ring to its appropriate
deffect line in the bright field disc, preferably one that is not involved
in any dynamic diffraction. Iddeally, use a C2 aperture that give you CBED
disks just smaller than the smallest reciprocal lattice spacing.

Regards,

Larry

---------------------------------------------------------------
Dr L. P. Stoter Techical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: Larry-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
---------------------------------------------------------------






From: kelloes-at-emlab.cb.uga.edu
Date: Mon, 23 Sep 1996 10:23:18 +0000
Subject: Hummer Sputter Coater-Thank you

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Message-Id: {199609231313.IAA01948-at-Sparc5.Microscopy.Com}

To all subscrubers who were interested in our Hummers:

Thank all of you who showed an interest in our Hummers. They have
been acquired, and I appreciate the great response I received.
Thanks again, Cathy Kelloes




From: Jbarjr-at-aol.com
Date: Mon, 23 Sep 1996 11:59:22 -0400
Subject: Microscopist Subscription

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Please subscribe me to your internet

Subscribe jbarjr-at-aol.com

Thank You,

Joseph G. Barney
President
Micro-Analytical Service Center, Inc.




From: Ford Tovey :      toveyf-at-calgary.associated-eng.com
Date: Mon, 23 Sep 1996 10:00:12 -0400
Subject: Info on Vickers LMs

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X-Mailer: Novell GroupWise 4.1

Fellow Microscopists:

Does anyone have information on how to contact
Vickers, the UK-based manufacturer of light
microscopes? I'm in Calgary, Canada.

All help is appreciated.

Thanks!

Ford Tovey {toveyf-at-calgary.associated-eng.com}





From: Fangqiong Lu :      fanglu-at-maestro.geo.utexas.edu
Date: Mon, 23 Sep 1996 09:53:48 -0500
Subject: polish metal probe standard

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To: Microscopy-at-Sparc5.Microscopy.Com (Microscopy Listserver U.S.A.)

Dear All,

I have two electron microprobe standard mounts of metals that need to be
repolished. Some of the metals are very chemically reactive so the mounts
have been kept under vacuum in a dessicator. One mount includes Mg, Al, si,
Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Cu, Zn, Zr,Nb, the other includes Zn, Ge, As,
Se, Cd, In, Sn,Te,Tl,Pb,Bi,Th,U,Ag,Au,ru,Rh,Pd,Os,Ir,Pt. Any advice on how
to go about polishing them would be sincerely appreciated.

Fangqiong Lu
Analytical laboritories
Department of Geological Sciences
The University of Texas at Austin
voice:512-471-5847
fax:512-471-9425





From: VayTek, Inc. :      vaytek-at-netins.net
Date: Mon, 23 Sep 1996 13:48:03 -0500 (CDT)
Subject: Re: Digital Image: B/W to B/color

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X-Nupop-Charset: English

} The idea is to use a particular color, rather than white, to represent the
} presence of an element in an EDS dot map. Is there a way to convert a B/W
} digital image to B/color image using either Photoshop or NIH-Image? Thanks
} for any input.
}
} Michael Cinibulk

I am a vender replying to this inquiry. Since you mentioned NIH-Image, I
presume you are interested in viewing your image as a 3D reconstruction.
Our volume visualization software allows full control of color and opacity.
With VoxBlast's Palette Editor, you can asign any value of each of the three
primary color to any portion of the image's histogram. In other words, you
can asign any color to any portion of the image's gray scale.

Furthurmore, you get full control of the image's opacity. This means that
you can make selected portions of the image transparent in order to view a
component inside.

I hope this is helpfull.

Regards

Patrick Guerin
Technical Support Engineer
VayTek, Inc.
Suite 109
305 West Lowe Avenue
Fairfield Iowa 52556

Tel : 515 472-2227
Fax : 515 472-8131
E-Mail :pguerin-at-vaytek.com





From: VayTek, Inc. :      vaytek-at-netins.net
Date: Mon, 23 Sep 1996 11:54:24 -0500 (CDT)
Subject: Re: Digital Image: B/W to B/color

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} The idea is to use a particular color, rather than white, to represent the
} presence of an element in an EDS dot map. Is there a way to convert a B/W
} digital image to B/color image using either Photoshop or NIH-Image? Thanks
} for any input.
}
} Michael Cinibulk

I am a vender replying to this inquiry. Since you mentioned NIH-Image, I
presume you are interested in viewing your image as a 3D reconstruction.
Our volume visualization software allows full control of color and opacity.
With VoxBlast's Palette Editor, you can asign any value of each of the three
primary color to any portion of the image's histogram. In other words, you
can asign any color to any portion of the image's gray scale.

Furthurmore, you get full control of the image's opacity. This means that
you can make selected portions of the image transparent in order to view a
component inside.

I hope this is helpfull.

Regards

Patrick Guerin
Technical Support Engineer
VayTek, Inc.
Suite 109
305 West Lowe Avenue
Fairfield Iowa 52556

Tel : 515 472-2227
Fax : 515 472-8131
E-Mail :pguerin-at-vaytek.com





From: VayTek, Inc. :      vaytek-at-netins.net
Date: Mon, 23 Sep 1996 11:54:24 -0500 (CDT)
Subject: Re: Digital Image: B/W to B/color

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


} The idea is to use a particular color, rather than white, to represent the
} presence of an element in an EDS dot map. Is there a way to convert a B/W
} digital image to B/color image using either Photoshop or NIH-Image? Thanks
} for any input.
}
} Michael Cinibulk

I am a vender replying to this inquiry. Since you mentioned NIH-Image, I
presume you are interested in viewing your image as a 3D reconstruction.
Our volume visualization software allows full control of color and opacity.
With VoxBlast's Palette Editor, you can asign any value of each of the three
primary color to any portion of the image's histogram. In other words, you
can asign any color to any portion of the image's gray scale.

Furthurmore, you get full control of the image's opacity. This means that
you can make selected portions of the image transparent in order to view a
component inside.

I hope this is helpfull.

Regards

Patrick Guerin
Technical Support Engineer
VayTek, Inc.
Suite 109
305 West Lowe Avenue
Fairfield Iowa 52556

Tel : 515 472-2227
Fax : 515 472-8131
E-Mail :pguerin-at-vaytek.com





From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Mon, 23 Sep 1996 12:31:38 -0600
Subject: cryofix/propane and copper

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Greetings,
Does anyone know if liquid propane and copper can interact to
cause a precipitate of some kind? I have a device for plunge freezing in
which propane is liquified in a well formed by a copper pipe, sealed at one
end. Between runs, when the unit is at room temp and the propane has
evaporated, I have found a conspicuous amount of blackish brownish powder
left in the well. Is it possible that the propane and copper are reacting
in some way? Does anyone out there have a similar unit in which copper and
propane do not cause any powdery residue? Is there reason to think that
some common impurity in copper could be the culprit? Any advice here
welcome. The powdery residue seems to damage knives, so we need to prevent
its forming.
Thanks very much,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / University of Missouri
/ | / / \ / / Biological Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
/ / / \ \ / voice: 573-882-0173
/ /____ / \ \____/ /_____ fax: 573-882-0123






From: roy-at-bayou.uh.edu (Roy Christoffersen)
Date: Mon, 23 Sep 1996 13:25:31 -0500
Subject: HRTEM multislice: persistent "white" atoms

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I would appreciate input from the those of you out there doing HRTEM image
simulations concerning the following problem. I am running simulations
using the Cerius HRTEM image simulation package on a Sr-Co oxide structure.
In the zone axis projection I am working on there is a "high projected
potential" atomic column where Sr and Co are exactly on top of one another,
and adjacent "lower projected potential" columns where these cations do not
quite line up. For the first broad CTF interval beyond Schertzer where the
CTF is supposed to produce "white" atoms everything is fine: the
high-potential column shows up as the brightest (white) atomic column of
the bunch.

However, at or near Schertzer where the contrast reverses and atoms are
supposed to be black, the high-potential column is not the "blackest" of
the bunch but rather persists as a fairly bright white spot, even though
other lower-potential columns in the same atomic row are now black. Why
didn't the contrast for the high potential column "reverse" like the
others? I have checked my convergence parameters, e.g. slice thickness, #
beams etc. and they seem fine. The effect is most noticable for a thickness
of 85 A, but is still there for thicknesses below this. What gives? Is
there some thickness effect here that I'm not aware of? Am I guilty of some
naive notion about HR imaging? Any thoughts from you image simulators out
there? Thanks in advance.

Roy Christoffersen
Texas Center for Superconductivity
3201 Cullen
Houston, TX 77204-5932
roy-at-bayou.uh.edu
(713) 743-8273
FAX: (713) 743-2787






From: Roy Christoffersen
Date: 9/23/96 5:31 PM
Subject: Re: HRTEM multislice- persis

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Message-Id: {n1368612402.1449-at-macmail7.lbl.gov}

However, at or near Schertzer where the contrast reverses and atoms are
supposed to be black, the high-potential column is not the "blackest" of
the bunch but rather persists as a fairly bright white spot, even though
other lower-potential columns in the same atomic row are now black. Why
didn't the contrast for the high potential column "reverse" like the
others? I have checked my convergence parameters, e.g. slice thickness, #
beams etc. and they seem fine. The effect is most noticable for a thickness of 85 A, but is still there for thicknesses below this.
What gives? Is there some thickness effect here that I'm not aware of? Am I guilty of some naive notion about HR imaging? Any
thoughts from you image simulators out there? Thanks in advance.

Roy Christoffersen
Texas Center for Superconductivity
3201 Cullen
Houston, TX 77204-5932
roy-at-bayou.uh.edu
(713) 743-8273
FAX: (713) 743-2787

:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:
Michael A. O'Keefe, Deputy Head
National Center for Electron Microscopy
Lawrence Berkeley National Laboratory
University of California
Berkeley, California 94720
tel: (510) 486-4610
fax: (510) 486-5888
email: maok-at-lbl.gov
http://ncem.lbl.gov/
:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:








From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Tue, 24 Sep 1996 07:55:47 +0100
Subject: Re: cryofix/propane and copper

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To: Microscopy-at-Sparc5.Microscopy.Com (Microscopy Listserver U.S.A.)
Microscopy-at-Sparc5.Microscopy.Com

} Greetings,
} Does anyone know if liquid propane and copper can interact to
} cause a precipitate of some kind? I have a device for plunge freezing in
} which propane is liquified in a well formed by a copper pipe, sealed at one
} end. Between runs, when the unit is at room temp and the propane has
} evaporated, I have found a conspicuous amount of blackish brownish powder
} left in the well. Is it possible that the propane and copper are reacting
} in some way? Does anyone out there have a similar unit in which copper and
} propane do not cause any powdery residue? Is there reason to think that
} some common impurity in copper could be the culprit? Any advice here
} welcome. The powdery residue seems to damage knives, so we need to prevent
} its forming.
} Thanks very much,
} Tobias Baskin

I don't have any specific experience of this problem, but a point that may
be relevant is that there are a huge number of copper-based 'alloys', many
of which appear to be copper only.

I came across this problem with a low temperature TEM holder which would
not cool down properly. After a lot of time, it turned out that for reasons
on better machining properties a previously un-tried 'copper' had been used
in the construction. It did have good machining properties but its thermal
conductivity was alomost as bad as stainless steel.

I guess the point is, that your copper may not be quite what you think it
is and the propane is reacting not with the copper but an alloying
component, so among other things, I check exactly what is in your 'copper'
pipe.

Regards,
Larry

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: Larry-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
---------------------------------------------------------------






From: Dave King (607)757-1248 T37/257-3 Ext DEKING-at-VNET.IBM.COM
Date: 24 Sep 1996 08:38:29 EDT
Subject: Re: cryofix/propane and copper

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Message-Id: {199609241231.HAA04445-at-Sparc5.Microscopy.Com}

To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}
*** Reply to note of 09/24/96 04:37

Tobias,

I'd say you probably have a corrosion problem, with condensed
moisture, but there must be an additional corrodent present,
unless your pipe is made from some strange alloy which is not
very corrosion resistant. Is there a way you can check for
moisture about the time all the propane has evaporated out? I'll
bet you'll stop the problem if you can backfill with a dry gas,
or otherwise keep the pipe dry.

. {
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: Beverly E Maleeff
Date: 24 Sep 96 10:05:57 EDT
Subject: October '96 PSM Meeting

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Message-Id: {9609241706.AA7020-at-pho903.sbphrd.com}
To: microscopy {microscopy-at-Sparc5.Microscopy.Com}
{Beverly_E_Maleeff-at-sbphrd.com}

PHILADELPHIA SOCIETY FOR MICROSCOPY
MEETING NOTICE
October 1996

DATE: Tuesday, October 8, 1996

PLACE: Laboratory for the Research of Science and Materials (LRSM) Building,
33rd and Walnut Street. Parking is available behind
the LRSM Building after 5:00 PM.

TIME: 5:30 - 7:30 PM Social hour
Dinner will be informal and available throughout the
social hour.

7:30 PM Speaker


Basic Principles and Industrial Applications in Atomic Force Microscopy

Dr. Ray Eby
FocalPoint Instruments
Glen Gardner, NJ

The main functions of an Atomic Force Microscope (AFM) will be discussed,
including: (I) the primary components of the instrument; (ii) some of the
various scan modes that are available; (iii) the types of data that are
available; (iv) a variety of mainstream applications for this relatively new
technology.

The AFM is primarily a high resolution surface imaging tool. It is a
lensless microscope that profiles the surface with a sharp stylus. A 3D
image is constructed from a series of parallel rastered line scans. Surface
roughness, frictional coefficients and magnetic domains are some of the
properties that can be imaged with this tool. The AFM is also a tactile
tool for probing elasticity and adhesion.

************************************************************************************************

Reservations will be taken by Ms. Pat Overend at the University of Pennsylvania,
215/898-8337. Deadline for reservations will be Friday, October 4.
Cancellations must be received by Ms. Overend no later than 5:00 PM, October 4,
1996.

DINNER:

Members $12.00 Student members $6.00 Non-members $15.00

Menu: Mexican beer
Salsa, chips, pretzels, etc.

Vegetarian chili
Taco shells, crispy or soft
Sauteed ground beef or chicken
Shredded lettuce
Grated cheese
Diced tomatoes
Sour cream
Guacamole
Refried beans
Tortilla chips

Fresh fruit salad

Coffee, decaf or tea



If you have any questions regarding the meeting please feel free to contact
Rollin
Lakis of the Executive Council at 215/898-8718 or lakis-at-lrsm.upenn.edu





From: ebs-at-ebsciences.com
Date: Tue, 24 Sep 1996 09:44:56 -0500
Subject: Re: polish metal probe standard

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Dear Dr. Lu and colleagues-

We (Energy Beam Sciences) represent MicroAnalytical Consultants in the
United States. They (MAC) can repolish old microanalysis standards and
return them to their original condition. The cost is very reasonable.

Best regards,
Sonja L. White, Sales & Marketing Secretary
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: smithg-at-gar.union.edu (George W. Smith)
Date: Tue, 24 Sep 1996 14:02:44 -0500
Subject: Unsubscribe

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Please Unscribe.







From: Helen Campbell :      HELEN-at-minmet.lan.mcgill.ca
Date: Tue, 24 Sep 1996 15:05:15 EST5EDT
Subject: Image format conversions

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Message-Id: {199609241909.PAA11118-at-sirocco.CC.McGill.CA}

Hello!
I would like to convert images aquired by our Noran I2 system
(i.e. from Fabled Flextran!) to Tif format. Can anyone tell me how to
do it?
Helen




From: Leo Marin :      leo-at-spine.med.utoronto.ca
Date: Tue, 24 Sep 1996 15:16:37 -0400 (EDT)
Subject: re curling of bio. specimens

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Hi everyone,

I received 4 replies to my request for suggestions on reducing or
preventing curling of specimens during TEM processing:

... pin down specimens throughout entire processing

... sandwich specimen between large pore membranes in a swinnex filter
assembly

... very slow dehydration (Fur-jiang Leu et al. (1993), Lab Invest'n V.69
pp121-130) by delivering dehydrating agents with a slow peristaltic pump

... use of a "true Enviromental SEM"

Your suggestions are truly appreciated.

Leo Marin




From: EMLAB-at-vet.ksu.edu
Date: Tue, 24 Sep 1996 17:11:41 CST6CDT
Subject: H-300 TEM/H-3010 SEM

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HI!
If your the proud owner of a H-300 with the H-3010 SEM accessory,
will you please reply! I have a few quick questions.
Thanks in advance!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!




From: Darryl Krueger :      giba-at-puccini.crl.umn.edu
Date: Tue, 24 Sep 1996 17:33:21 -0500
Subject: MINNESOTA MICROSCOPY MEET

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MINNESOTA MICROSCOPY SOCIETY, PRESENTS:

FALL BUFFET DINNER & TOUR
THURSDAY, OCTOBER 3, 1996

TOUR: Cryo EM & B.I.P.L.
5:45 - 7:00 PM
JACKSON HALL, U. of M, Mpls. East Campus

BUFFET DINNER
CAMPUS CLUB, 7:00 - 8:30 PM
Mpls. East Campus, UM

SPEAKER: Paul Walther, Zurich
TOPIC: High Resolution Cryo Field Emission SEM - New Applications & Methods?

MMS will hold its eighth annual Fall Buffet Dinner on October 3, 1996, at the
University of Minnesota's Campus Club, 401 Coffman Memorial Union, 300
Washington Avenue S.E., Minneapolis Campus(see map below). We hope to provide a
pleasant evening during which microscopists will be moved to renew or begin
memberships in MMS, MSA and/or MAS.

Different this year is a tour from 5:45-7:00 to begin the evening. The buffet
dinner and program follow from 7:00-8:30. The Campus Club sets up one of the
finest food lineups in the world. The dinner affords an excellent opportunity to
meet microscopists from many disciplines, talk shop, and to have a pleasant time
together. The talk is from 7:30-8:30. Parking is available behind the Union in
the East River Road Ramp (connected by walkway to Union) for $2.50 (per day
rate), and at other Minneapolis Campus locations(see map inside).

Our featured speaker, Paul Walther, is from the ETH, Laboratory for Electron
Microscopy 1, Institute for Cell Biology, Zurich, Switzerland.

Before the Campus Club Buffet, the Department of Cell Biology and Neuroanatomy
will host an open house from 5:45 to 7:00pm with demonstrations of CryoSEM in
Stan Erlandsen's lab, CryoTEM in Ed Egelman's lab, Cryo-sectioning by Tom
Groppoli and a tour of the Biomedical Imaging Processing Laboratory (BIPL).
People should come to room 2-164 in Jackson Hall (basement level) at 5:45pm
where we will do the microscopy demos after which we will see the imaging lab on
the fourth floor. The tour will last about 60 minutes and we'll proceed directly
to the Campus Club upon its conclusion.

We will hold a short business meeting just before the talk. The Buffet Dinner is
$12.00 per person payable at the door. (non-member $22.00, includes new
membership), $8.00 for current student members (non-member student $13.00,
includes new membership).

In order for us to provide an accurate head count to the Campus Club, please
make an advance reservation by contacting Mike or Stuart. Deadline: Monday,
September 30.

Buffet head counters:
Mike Coscio (612)569-1331, 569-1284 FAX, mike.coscio-at-medtronic.com
Stuart McKernan, (612)626-7942, 626-7530 FAX, stuartm-at-maroon.tc.umn.edu





From: Murphy, Judy :      murphy-at-sjdccd.cc.ca.us
Date: 24 Sep 1996 18:19:28 -0800
Subject: NCSM Meeting Oct. 3, 1996

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Delta Microscopy Society and Northern California Microscopy Society
Meeting Announcement

When: October 3, 1996 from 8 am -9:30 pm

Where: San Joaquin Delta College, Microscopy Technology Center
Stockton, California

Deadline to let us know: Call by September 27, 1996 Friday at 5pm
Contact Judy Murphy, numbers and e mail below.

Cost: $15 for daytime workshops (Make check out to Delta Microscopy Society
and send to Judy Murphy, address below)
$12.50 for dinner (Make check out to Northern California Microscopy
Society (NCSM), and send to Judy Murphy
$6.25 for dinner for students.

Overall Times
7:30-8:00 am Registration, Holt Lounge
8:00-4:45 Workshops/Lectures listed below, Reservations Required
4:45-6:15 Hosted Bar
6;15-7:30 pm Dinner, Reservations Required
7:30-9:15 pm Presentations, listed below in West Forum

Contact for further information and reservations:
Dr. Judy Murphy
Microscopy Technology Center
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207
Phone: 209/474-5284
FAX: 209/474-5600
e mail: murphy-at-sjdccd.cc.ca.us

PROGRAM
Microwave Workshops: 2 repeat sessions
8:00-11:00am
1:30-4:30pm
Lectures
Microwave Experiences and Applications
11:00-12:15 (These lectures will be given only once)
Applications of Microwaving in Biotechnology: Linda Rangell, Genentech, Inc.,
South San Francisco, CA
Experiences with Microwaving in EM Preparations: Dr. Kent McDonald, UC,
Berkeley
Applications of Microwaving in the Clinical Setting: Bob Munn, UC, Davis
Challenges in Microwaving for Immunocytochemistry: Greta Fry, UC, Davis

Virtual SEM Demo: in Microscopy Lab, Holt: (2 repeat sessions)
9:30 am and 4:00 pm


Tripod Polishing Including the Anatomy of the Integrated Circuit
Shane Roberts, South Bay Technology
8:00-9:00: Lecture
9-11:30 Hands-on Tripod Polishing with your own samples
1-2:00 Repeat Lecture
2:00-4:00 Repeat Hands-on Tripod Polishing

Acoustic Microscopy: How it works, appropriate samples, information output
and applications. Marti McCurdy, Sonix Labs
9:00-10:00 Lecture
10-11:30 Hands-on acoustic microscopy
2-3:00 Repeat Lecture
3:00-4:45 Hands-on acoustic microscopy

Atomic Force Microscopy, AFM, How It Works and Applications
Gary Williams, Topometrix
9-10:00 Lecture
10-11:30 Hands-on AFM. Try your own samples.
1:30-2:30 Repeat lecture
2:30-4:30 Hands-on AFM

Focused Ion Beam, FIB Demo on FEI 611 FIB in Microscopy Lab
9-11:00 am and 3:45-5 pm

Lectures
10:00-10:45 Characterization of Multi-layered Films by EM, Mark Wall,
Lawrence Livermore National Lab
10:45-11:30 Digital Imaging and Control in TEM: Dr. Jim Mancuso, Advanced
Microscopy Techniques
11:30-11:55 Vacuum Systems in EM, Ron Vane, XEI, Inc.

LUNCH 12-1

Lectures
1-1:45pm Focused Ion Beam Technology: Peter Carleson, FEI Corp., Hillsboro,
OR
1:45-2:15 FIB Techniques in the Semiconductor Industry: Ken Faulk, Intel,
Folsom, CA
2:15-2:45 Applications of FIB in the Disk Drive Industry: Russell Stearns,
Read-Rite, Inc., Fremont, CA
3:00-3:30 FIB and Mask Design in the Semiconductor Industry: Sutton Faulk,
Intel, Folsom, CA
3:30-3:45 TEM Stage Compatibility with FIB System: Mike Sherrill or Louis
Dawang, Hitachi Instruments, Mountain View, CA

4:30 Lab Tours

5-6:15 pm SOCIAL (Holt Lounge, hosted bar)
6:15-7:30 pm DINNER, Upper Danner

Lectures
7:30-8:00 pm 5D Microscopy Using Widefield Deconvolution, Practical
Considerations, Wallace Marshall, UC, San Francisco
8-8:30 pm Colorization of MIcroscopy Images Without Losing the 3D Effect:
Practical Aspects of How To Do It, Dr. Bob Anderhalt, Philips Electronics,
Mahwah, NJ
8:30-9:15 3D Reconstructions and the Space Program: Dr. Kevin Montgomery,
NASA Ames Research Center, Moffett Field, CA







From: Stephen J Murray :      smurray-at-u.Arizona.EDU
Date: Tue, 24 Sep 1996 22:30:37 -0700 (MST)
Subject: NCSM Meeting Oct. 3, 1996

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subscribe microscopy





From: m.colella-at-ansto.gov.au (Mike Colella)
Date: Wed, 25 Sep 1996 16:54:52 +0900
Subject: TEM specimen prep of Sn/Al

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Greetings,

Would anyone be able to suggest a method of preparing a TEM cross-sectional
sample of Tin coated Aluminium discs. The tin coatings range from
approximately 0.2 - 1 micron in thickness. We are interested in the Sn
phase chemistry at the interface, and therefore temperature variations are
critical. I have had no success with low angle ion milling techniques, as
the tin coating tends to melt.

Would tripod polishing, or microtoming be the answer?

Looking forward to many replies. (I hope)


Mike Colella,
Materials Division, ANSTO






From: VARGAS-at-lib.dote.hu
Date: Wed, 25 Sep 1996 12:05:36 +100
Subject: help

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help




From: Albert Sicignano :      mks-at-cyburban.com
Date: Wed, 25 Sep 1996 07:00:05 -0400
Subject: looling for sample ciater soytter system

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Message-ID: {324910B5.7681-at-cyburban.com}

I need a used small DC sputter Au/Pd sample coater. Any one have
a suggestion as to leads? thanks Mike Konas, mksas-at-cyburban.com




From: Davin Jutila :      DJUTILA-at-wpsmtp.siumed.edu
Date: Wed, 25 Sep 1996 09:08:42 -0600
Subject: Unsubscribe

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X-Mailer: Novell GroupWise 4.1

Unsubscribe djutila-at-wpsmtp.siumed.edu







From: Zhen Quan Liu :      wl-at-ibmstone.pku.edu.cn
Date: Wed, 25 Sep 1996 21:58:59 -0500
Subject: Edwards Pump EDM 6

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I would like to know the speed of Edwards pump of Model EDM 6 (Serial No.
05460). Our engineers want to make sure if it can replace another Edwards
pump (Model RV8, speed is 8 m3/hr).

Thanks in advance.

Z.Q. Liu (Ph.D)
zqliu-at-pku.edu.cn
EML, Peking Uni.
Beijing China
Tel: 86-10-6275-1427(office)
86-10-6275-3727(home)
Fax: 86-10-6275-1615(office)







From: Glenn Poirier :      glennp-at-eps.mcgill.ca
Date: Wed, 25 Sep 1996 11:46:15 -0400 (EDT)
Subject: Contamination rates

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Good morning all,
Does anyone know of a reference for information on carbon contamination
rates in microanalysis (i.e. effect of current, accelerating voltage,
spot size etc. )? I'm going to try to do some semi-quantitative
analyses of carbon in steel and am trying to find analytical conditions
which minimize contamination. Also, if anyone knows of a technique(s) for
cleaning samples before carbon analysis could they share it with me.

TIA

Glenn




From: Owen P. Mills :      opmills-at-mtu.edu
Date: Wed, 25 Sep 1996 11:50:35 -0400
Subject: TN5500 RGB monitor

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Message-Id: {199609251326.IAA07082-at-Sparc5.Microscopy.Com}
To: Microscopy messageslistserver {Microscopy-at-sparc5.microscopy.com}

Hi All,

I am relaying a message from a colleague below;

--------------------------------------------

} Our TN5500 monitor bit the dust. Please contact me at the address that
follows if } you have or know of a used 5500 RGB monitor for sale."
}
} Leonard Smith
} CIF Department, Tulane University
} New Orleans, LA
} 504-865-5173
} leonards-at-i-way.net

--------------------------------------------

Thanks in advance for your help in locating a monitor for him.


Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: Michael P. Webber :      102413.2660-at-CompuServe.COM
Date: 25 Sep 96 15:36:14 EDT
Subject: Raleigh NC Roadshow

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To anyone near to the Raleigh Vicinity.
LEO Electron Microscopy in collaboration with Oxford instruments, will be
displaying the LEO 435 Variable pressure sem and Oxford Isis microanalysis
system, Both systems work in The "Microsoft Windows" operating system
enviroment. The venue is the Brownstone hotel, adjacent to NC state University.
in downtown Raleigh. If anyone is interested in a introductory demonstration,
please reply either by e-mail or toll free # to:
Mike Webber
LEO Electron Microscopy
1-800-356-1090 Ext 708.
The instruments will be available for viewing by appointment on Tuesday Oct 8th
from 9:00am to 6:00pm, and Wednesday from 9:00am to 5:00pm.
Thursday and Friday will be dedicated to the Southern Area Forensics meeting
Thank You
Mike Webber





From: Visit Thaveeprungsriporn :      fntvtv-at-eng.chula.ac.th
Date: Wed, 25 Sep 1996 19:34:17 +0700 (TST)
Subject: Conference in Thailand

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ANNOUNCEMENT
******************************************************************************
Prince of Songkla University and the Electron Microscopy Society
of Thailand will hold the "XIVth Annual Conference on Electron
Microscopy" on Dec 11-13, 1996 at the J.B. Hotel, Hat Yai, Songkla, Thailand.

Several nationally and internationally renowned electron
microscopists are invited to give lectures in life science and materials
science.
Invited Lectures delivered by:
Materials Science:
Dr. H. Hashimoto - Okayama University of Science, Japan
Dr. K. Yada - Aomori Public College, Japan
Dr. J. Mansfield - University of Michigan, USA

Life Science:
Dr. M. Nakai - Osaka Medical College, Japan
Dr. H. Suzuki - Nihon University Surugadai Hospital, Japan
Dr. M. Osumi - Japan Women's University, Japan
Dr. R. Hori - Ogaki Women's College, Japan

Some world-wide companies will be introducing their new advanced
technologies in electron microscopy and accessories at the conference.

RMC, USA - Cryo UltraMicrotome for Immunocytochemistry at the EM
level. Sample Preparation Techniques for the Ultramicrotomy of materials
science specimens.

Gatan, USA - TEM Sample Preparation, Digital Imaging.

JEOL, Japan - Field Emission Electron Microscopy and Analytical
Electron Microscopy.

Oxford Instruments, U.K. - OPAL System
*****************************************************************************
For Enquiries and Correspondence

Submission of Paper, please contact:
Dr. Prapee Sretarugsa, Dept of Anatomy, Faculty of Science
Mahidol University, Bangkok 10400
Tel:(662)-246-1320, (662)-246-0063-Ext. 4072.
Fax: (662)-247-9880, (662)-247-7075

Deadline: Abstracts must be received before October 31, 1996

For Registration and Hotel Accommodation, please contact
Mr. Paiboon Nuannin, Faculty of Science, Prince of Songkla University
Tel:(66)(74)-211030 Ext.2617
Fax: (66)(74)-212817, (66)(74)-212920 Ext.104
E-mail: emstconf-at-ratree.psu.ac.th
******************************************************************************




From: Glenn Poirier :      glennp-at-eps.mcgill.ca
Date: Wed, 25 Sep 1996 11:46:15 -0400 (EDT)
Subject: Contamination rates

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Message-ID: {n1368463478.61461-at-mse.engin.umich.edu}
"Micros/contamination" {microscopy-at-Sparc5.Microscopy.Com}
X-Mailer: Mail*Link SMTP/QM 3.0.0GM

Reply to: RE} Contamination rates

I believe two general methods have been used to control contamination. One
involves inserting a very cold surface (usually cooled with LN2) into the
specimen chamber as near to the specimen as possible to condense out volatile
organic vapors before they reach the specimen. The second involves directing
a fine jet of oxygen onto the region of the surface being analyzed, so that
organic materials are 'burned' up, forming volatile compounds such as carbon
dioxide and water that are readily removed by the vacuum pumps.

The matter is discussed, with references, by Heinrich on p. 17 of his book
'Electron Beam Microanalysis', Van Nostrand-Reinhold, 1981. The book
bybGoldstein,et. al. 'Scanning Electron Microscopy and X-ray Micro Analysis'
also containd a dozen or so references to the contqmination problem.


--------------------------------------

TIA

Glenn

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From: rybicka-at-acsu.buffalo.edu (K.K rybicka)
Date: Wed, 25 Sep 1996 16:31:18 -0400 (EDT)
Subject: neglected organelles

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Hi, Microscopists,

In reply to my earlier display on glycogen I received an e-mail
which indicated to me that there is still great confusion among
microscopists about glycosomes. The basic points of this e-mail
are presented below in quotation marks.

First: "The original question was whether glycogen can be
contrasted in tissue for transmission electron microscopy".

Glycogen, a polymer made up of glucose units, can be shown
in TEM, by the use of histochemical, or negative staining techniques.
However, glycogen does not react with ionic compounds such as osmium,
uranium, and lead salts, or with the ferrocyanides as used by DeBruijn.
All these salts react with the proteins which are associated with
glycogen and which together with glycogen make up the structures called
glycosomes; but they do not stain glycogen itself. This protein
component was misinterpreted in early EM studies as glycogen and this
misinterpretation persists in textbooks, in research articles, and in
diagnostic pathology.

Second: "Why this is such an issue".

Serious problems eventually appear in research when one thing
is mistaken for another. In this case, when a complex protein component
visible in TEM is believed to represent glycogen. This misunderstanding
has, in fact, led to a dead end in the TEM studies on glycogen. Also, in the
last 20 years there have been almost no TEM studies on glycosomal
proteins whereas biochemical research has made enormous progress in the
identification of these proteins and the mechanisms of their activity.


Third: "...people still refer to glycogen as granules instead of
glycosomes, ....it may be simply a matter of semantics".

The problem is more than a matter of semantics. When we fail
to recognize the true nature of glycosomes as complex organelles which
undergo constant metabolic turnover and interprete them as simple,
deposits of a stored carbohydrate (still confusing the enzymatic complex
in these organelles with glycogen) we overlook very important aspects of
cell organization, metabolism, and function. For example, the existing
data show that some glycosomes are intimately associated with different
cellular structures whereas others are free in the cytoplasm. The meaning
of this observation has yet to be explored. The size and the staining
intensity of the protein component of glycosomes can vary depending on
the metabolic state of glycosome; but, do larger, or more densely
staining glycosomes indicate that there is more glycogen in the cell?
In pathology alterations in tissue glycogen are used as a diagnostic
feature in a number of diseases. Is there more to be learned about
disease processes by understanding the nature and functions of the
glycosome?

In order to begin to study all aspects of glycosomes as
functional cellular units, we first have to correct the existing
confusion in the TEM interpretation. The realization that we are
dealing with an organelle opens a vast field for research on the
cellular metabolism of glycogen with the use of modern molecular
and cellular biology techniques.

Readers interested in the subject are referred to a review article:
K.K.Rybicka, Glycosomes-the Organelles of Glycogen Metabolism, Tissue & Cell
1996, 28,(3) 253-265.

Krystyna Kielan Rybicka
__________________________________









From: Jintamas SUWANJARAT :      sjintama-at-ratree.psu.ac.th
Date: Thu, 26 Sep 1996 10:53:41 +0700 (GMT+0700)
Subject: neglected organelles

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Dear Sir, Please inform me where will the meeting for 16th ICXOM take
place. How can I get the circutar? Thanks.
Jintamas Suwanjarat





From: Jintamas SUWANJARAT :      sjintama-at-ratree.psu.ac.th
Date: Thu, 26 Sep 1996 10:53:41 +0700 (GMT+0700)
Subject: neglected organelles

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Dear Sir, Please inform me where will the meeting for 16th ICXOM take
place. How can I get the circutar? Thanks.
Jintamas Suwanjarat





From: Hans-Martin Vaihinger :      Hans-Martin.Vaihinger-at-rz.ruhr-uni-bochum.de
Date: Thu, 26 Sep 1996 09:37:28 +0000
Subject: Tissue handling for TEM fixation/embedding

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Comments: Authenticated sender is {vaihihbt-at-mailhost.rz.ruhr-uni-bochum.de}

Hello to all,

I am seeking for inputs how to handle small delicate pieces of tissue during
the preparation/fixation/embedding process for TEM. There must be
something beside using fine forceps, spoons or toothpicks. What about
agar-embedding? How good is fixative penetration through agar?
Any suggestions will be welcome!

Hans-Martin

**************************************************************
Hans-Martin Vaihinger
Ruhr-University of Bochum
Comparative Endocrinology Research Section
Building ND 5/37
44780 Bochum
GERMANY
*********************************************************
phone ++49 234 700 4329
fax ++49 234 709 4551
email Hans-Martin.Vaihinger-at-RZ.Ruhr-Uni-Bochum.de




From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Thu, 26 Sep 1996 08:55:57 -0500
Subject: Contamination Reduction

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To add to Will Bigelow's Comments:

Cooling the specimen also helps alot more. In the AEM if you cool the sample
in LN2 stage to at least -30 C you will virtually eliminate
contamination. The H20 parital pressure, in the vicinity, is the
factor which helps. As the H20 molecule is broken up by the interaction
with the beam, the freed O reacts with C and forms CO2 and is
pumped away, just as Will Bigelow'shas suggested, but it is
more efficient as you are now forming the O closer to the area
that you want to keep clean. However if you have a
very dirty vacuum system this may not be sufficient. I have used this
method for years in TEM's (actually come to think of it since ~ 1978 )
and it works well, unless you have a lot of carbon
in your sample. The problem here should be obvious, the O will
also react with your sample and you will "remove" it. I've drilled
some nice holes in diamond with a 100 kV electron beam in a
JEOL 100 C! There are a variety of manufacturer's of EM cold stages
so this might be a quick solution if you have access to such a stage.

Lists of commerical manufacturers of Microscopy related equipment and
accessories can be found at:

http://www.msa.microscopy.com/MSADocs/MSASustainingMembers95.html

or at

http://www.amc.anl.gov/#NatIntSites.



If your contamination is specimen borne, then
an alternative is to condition/clean the specimen AND the stage
with a reactive gas sputtering system. The gas reacts any residual organic
material on the surface of the specimen which was left by
any sample prep procedures (polishing, washing, solvent cleaning etc..)
or poor storage conditions (gelatin capsule storage, dirty stages....) .
I have also used this method for awhile in AEM work (on Steels as well
as lots of other materials ) and it works well for me.
You will need to chooze the correct gas but an Argon/Oxygen
mixture usually works for most materials.

There are no publications on this that I can give you as ANL submitted
a patent application (a few years ago) and hence put a hold on any
publications related to the
technique as applied to AEM. The patent was recently granted #5510624, and
there are now a few commerical EM accessory firms that have licensed the
technology
and are making a unit which should work well. If you are trying to
do TEM-based work then this may be another route for you. At least
two of the model designs that I am familiar with will also
handle specimens which are SEM size and could additionally help in that area.

If it is not obvious, I should state that my employer (ANL) has a financial
interest in the latter technique as they hold the patent. It would be
inappropriate for me to post the names of any specific manufacturers because
of that fact, but they are US companies so you should have no problems
also finding them on the WWW at the addresses listed above.



Nestor
Your Friendly Neighborhood SysOp






From: James J. McGee :      mcgee-at-epoch.geol.sc.edu
Date: Thu, 26 Sep 1996 09:38:41 -0400
Subject: Contamination rates

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Message-Id: {01BBAB8E.81A80AC0-at-summer.geol.sc.edu}

Beaman and Isasi (ASTM STP 506) looked at C contamination rates and its =
causes in 1972. Borile and Garulli (X-Ray Spectrometry, v. 7, 1978) =
discussed various mods to improve/allow C measurement in Fe-Ni alloys. =
Presumably there are more recent studies as well. Goldstein et al.'s =
Scanning Electron Microscopy & X-Ray Microanalysis book should have some =
info.
-Jim McGee

*.*.*.*.*.*.*.*.*.*.*.*.*.*.*.*.*.*.*.*.*
James J. McGee (jmcgee-at-sc.edu)
Dept. of Geological Sciences
University of South Carolina (803) 777-6300 (Office)
Columbia, SC 29208 (803) 777-6610 (Fax)


**************************original =
Message*****************************************
Does anyone know of a reference for information on carbon contamination=20
rates in microanalysis (i.e. effect of current, accelerating voltage,=20
spot size etc. )? I'm going to try to do some semi-quantitative=20
analyses of carbon in steel and am trying to find analytical conditions=20
which minimize contamination. Also, if anyone knows of a technique(s) =
for=20
cleaning samples before carbon analysis could they share it with me.

Glenn (Poirier)





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 26 Sep 1996 11:52:17 -0400
Subject: RE- Contam & Turbo pumps

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Message-ID: {n1368383521.69753-at-mse.engin.umich.edu}

Subject: Time: 11:34 AM
OFFICE MEMO RE: Contam & Turbo pumps Date: 9/26/96

Alfred Kracher asked whether or not there is a need to use direct methods to
control contamination problems in systems that are pumped with oil-free pumps
such as turbomolecular or cryogenic pumps.

I guess my answer would be, 'if it aint broke don't fix it. Certainly a
well designed and properly managed oil-free system should allow analyses to
be performed without problems arising from carbon contamination - - -}
UNLESS! the contaminating materials are introduced into the situation on or
by the specimen itself. This is a matter that is discussed at some length in
Section 2.10.4d, p. 74, of my book, 'Vacuum Methods in Electron Microscopy',
and so I will not go into details about it here except to comment that it can
cause significant contamination problems, even in the best of vacuum systems.

W. C. Bigelow (bigelow-at-umich.edu)





From: DDKJoe-at-aol.com
Date: Thu, 26 Sep 1996 12:43:11 -0400
Subject: Re: TEM specimen prep of Sn/Al

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Dear Mike:

I've done a lot of ultramicrotomy (UM) of metals and find soft ones like Sn
and Al fairly straightforward. Naturally, I'm trying to show the advantages
of UM in general and UM with a diamond knife specifically.

We are called upon periodically to demonstrate the feasibility of
ultramictomy of various materials in our lab here. We would be happy to work
with your sample and (hopefully!) provide a grid for you.

Just send a small bit with whatever instructions you think are appropriate to
the address below. We should have some preliminary results in a few days.

Regards,
Joe Tabeling
Delaware Diamond Knives
3825 Lancaster Pike
Wilmington, DE 19805
800-222-5143
302-999-8320:FAX
http://www.ddk.com




From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Thu, 26 Sep 1996 12:58:42 -0400 (EDT)
Subject: Re: Tissue handling for TEM fixation/embedding

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Dear Hans-Martin,

We routinely embed culture cells in agarose, dehydrate and embed. We do
this to hold them together during the embedding process, but i am sure it
would work to protect delicate tissues also. Let me know if you would like
a more detailed protocol.

Cheri Owen
Detroit Neurotrauma Institute
Wayne State University
Detroit, Mi

On Thu, 26 Sep 1996, Hans-Martin Vaihinger wrote:

} Hello to all,
}
} I am seeking for inputs how to handle small delicate pieces of tissue during
} the preparation/fixation/embedding process for TEM. There must be
} something beside using fine forceps, spoons or toothpicks. What about
} agar-embedding? How good is fixative penetration through agar?
} Any suggestions will be welcome!
}
} Hans-Martin
}
} **************************************************************
} Hans-Martin Vaihinger
} Ruhr-University of Bochum
} Comparative Endocrinology Research Section
} Building ND 5/37
} 44780 Bochum
} GERMANY
} *********************************************************
} phone ++49 234 700 4329
} fax ++49 234 709 4551
} email Hans-Martin.Vaihinger-at-RZ.Ruhr-Uni-Bochum.de
}





From: Patty Jansma :      plj-at-manduca.neurobio.arizona.edu
Date: Thu, 26 Sep 1996 09:51:00 -0600 (MDT)
Subject: Re: Tissue handling for TEM fixation/embedding

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{Pine.SUN.3.91.960926092504.4964C-100000-at-manduca.neurobio.arizona.edu}
MIME-version: 1.0
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We use low melting point agar for EM processing. The concentration varies
depending on the tissue-- usually between 2-7%. We fix first and then embed
in agar. The tissue then is run up as standard blocks of tissue.

Patty Jansma Tel:602-621-6671
plj-at-manduca.neurobio.arizona.edu
Arizona Research Labs Division of Neurobiology
University of Arizona

On Thu, 26 Sep 1996, Hans-Martin Vaihinger wrote:

} Hello to all,
}
} I am seeking for inputs how to handle small delicate pieces of tissue during
} the preparation/fixation/embedding process for TEM. There must be
} something beside using fine forceps, spoons or toothpicks. What about
} agar-embedding? How good is fixative penetration through agar?
} Any suggestions will be welcome!
}
} Hans-Martin
}
} **************************************************************
} Hans-Martin Vaihinger
} Ruhr-University of Bochum
} Comparative Endocrinology Research Section
} Building ND 5/37
} 44780 Bochum
} GERMANY
} *********************************************************
} phone ++49 234 700 4329
} fax ++49 234 709 4551
} email Hans-Martin.Vaihinger-at-RZ.Ruhr-Uni-Bochum.de
}




From: SilverStf-at-aol.com
Date: Thu, 26 Sep 1996 16:00:48 -0400
Subject: dry silver printer paper

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Hi everyone:

Does anyone know of a supplier for the special paper for an old dry silver
printer? My old vendor has vanished and I need to get a fresh supply of
paper. Thanks for any help anyone can lend.

Anne Esposito
E.M. Connection




From: VCRVINCE-at-aol.com
Date: Thu, 26 Sep 1996 16:11:41 -0400
Subject: XTEM

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VCR GROUP 250 East Grand Avenue Ste. 70 415 875-1000
Incorporated South San Francisco, CA 94080 800 536-1827
Fax 415 875-7111


September 25, 1996


TO: Mike Colella

FROM: Vince Carlino

TOPIC: Sn/Al XTEM


Dear Mike,


If you send your address I will forward a procedure to dimple your samples
with D500i, DIMPLER. A DIMPLER user has very successfully dimpled Pb/Sn and
copper specimens to near electron transparency. In a few instances he did
not even ion mill samples. Therefore no heat. Your specimens will work just
as well.


Best Regards,


Vince Carlino





From: lamiller-at-uiuc.edu (Lou Ann Miller)
Date: Thu, 26 Sep 1996 11:05:13 -0500
Subject: Re: Tissue handling for TEM fixation/embedding

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Hans-Martin,

I "handle" small pieces of tissue by cutting off the tip of a plastic
dispopipet, and pipeting the samples from vial to vial, or from cutting
petridish to vial etc.

I have a little microcentrifuge. If samples go into suspension, just
before any chemical change, I gently centrifuge down the sample and then
change the chemical. And, then resuspend the sample. So the entire
processing is done in a 1.5 or 2.0 ml microcentrifuge tube.

At the end, we place small beam capsules inside clean 1.5 ml centrifuge
tubes, spin well, and polymerize the whole assembly as one. Taking it
apart when the blocks are hard.

This works for us.

Lou Ann



} Hello to all,
}
} I am seeking for inputs how to handle small delicate pieces of tissue during
} the preparation/fixation/embedding process for TEM. There must be
} something beside using fine forceps, spoons or toothpicks. What about
} agar-embedding? How good is fixative penetration through agar?
} Any suggestions will be welcome!
}
} Hans-Martin
}
} **************************************************************
} Hans-Martin Vaihinger
} Ruhr-University of Bochum
} Comparative Endocrinology Research Section
} Building ND 5/37
} 44780 Bochum
} GERMANY
} *********************************************************
} phone ++49 234 700 4329
} fax ++49 234 709 4551
} email Hans-Martin.Vaihinger-at-RZ.Ruhr-Uni-Bochum.de

***********************
Lou Ann Miller
Microscopic Imaging Laboratory
College of Veterinary Medicine
University of Illinois
2001 S Lincoln Ave
Urbana, Illinois 61801
217-244-1566
lamiller-at-ux1.cso.uiuc.edu

Microscopic Imaging Lab Home Pages:
http://www.cvm.uiuc.edu/MicImagLab/MicImagLab.html

Central States Microscopy Society
http://www.cvm.uiuc.edu/Homepages/LouAnnMiller/CSMS/csms.html

Personal Home Pages:
http://www.cvm.uiuc.edu/Homepages/LouAnnMiller/LAM.html
***********************






From: Paolo Ghiara :      ghiara-at-sienanet.it
Date: Thu, 26 Sep 1996 23:28:16 +0200
Subject: live video microscopy

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Message-Id: {324AF570.156E-at-sienanet.it}

Hello friends,
I would like to study live interaction between mammalian cells and
bacteria by time-lapsed video microscopy. Does anybody can give me a
hint on how and where I could find details to build or buy a chamber for
mantaining cells and bacteria alive under the microscope?
thanks for help.
P.Ghiara




From: GVKM07A-at-PRODIGY.COM ( CHARLES A GARBER)
Date: 26 Sep 96
Subject: Sn coated aluminum discs

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Message-Id: {199609261531.LAA16406-at-mime2.prodigy.com}
X-Mailer: Prodigy Internet GW(v0.9beta) - ae02dm02sc04

-- [ From: Charles A. Garber, Ph. D. * EMC.Ver #3.0 ] --

In response to the following:
=========================================
}
} Would anyone be able to suggest a method of preparing a TEM cross-
sectional
} sample of Tin coated Aluminum discs. The tin coatings range from
} approximately 0.2 - 1 micron in thickness. We are interested in the Sn
} phase chemistry at the interface, and therefore temperature variations
are
} critical. I have had no success with low angle ion milling techniques,
as
} the tin coating tends to melt.
===========================================

This can be done, quite easily in fact, if experienced, with diamond
knife ultramicrotomy. The Al is "soft" and unless the Sn layer is
extraordinarily thick, one should not have difficulty sectioning the
system. While one might think of using a "blunter" knife angle (e.g. 55
deg. was suggested), remember that compression and other undesirable
effects are more likely, and we therefore recommend a more "normal"
angle, such as 45 deg. Also, with the blunter angle, it would be more
likely that the Sn layer would separate during the sectioning. But you
do not have to spend big bucks for a so-called "life science" diamond
knife, save money buy using a "materials science" diamond knife, which
comes standard with a 45 deg. angle (SPI knives). After all, for those
who believe a life science knife is more desirable even for this kind of
work, remember that after the first "slice", the edge is now no better
than an SPI materials science diamond knife would have been anyhow!

After the thickness of the Sn layer exceeds some point, then it becomes
more difficult to section. However, that cut off point will be a higher
number in thickness with the smaller angle knife.

There are a few other "problems" you might encounter such as separation
of the Sn layer from the Al substrate. Sometimes it is better to embed
the top surface, and then positioning the sample in the microtome so
the cutting is 90 deg. to the surface instead of cutting along the
surface. But in the end, you should be able to get a very excellent
view of the structure of the Sn coating, including precise grain size
measurements.

If you are looking for signs of possible pin-holes in the Sn layer, then
before embedding, do a Pt sputtering (Au will work as well but is smears
easier). Then from the micrographs, you can follow that metallization
layer and see if it actually "goes" down into what could be a pin hole,
because there could always be the danger otherwise that what was being
interpreted as being a pin-hole would have been just where the knife
pulled out a piece of the coating (e.g. an artifact).

Information about SPI Materials Science Diamond Knives is available from
the SPI website given below.

Chuck

PS: One other comment: I am surprised to hear that melting occurred
during ion milling, and while we believe thin sectioning is the better
way to do this kind of sample, consider the following:

Maybe the answer is as simple as using a more conductive kind of
adhesive to be sure the sample is in good thermal contact with the
cooling effect of the sample holder. Two possiblities here, one being
our SPI Silver Paste Plus and the other being a new product that is
about to be introduced, namely a diamond support ring (and also a slot
for smaller samples). Diamond has heat conduction characteristics about
four times higher than copper.


======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: GVKM07A-at-prodigy.
com
West Chester, PA 19381-0656 USA Customer Service: spi2spi-at-2spi.
com


Look for us!
############################
WWW: http://www.2spi.com
############################
=====================================================




From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Thu, 26 Sep 1996 14:29:50 -0500 (CDT)
Subject: TEM Available

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We have an Hitachi HU 11B Transmission Electron Microscope in need
of a new home. The microscope is in working order, and has MANY spare
parts. For additional information, please contact me.


Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816







From: B.Cribb-at-mailbox.uq.oz.au (Bronwen Cribb)
Date: Fri, 27 Sep 1996 08:42:25 +1000
Subject: Re small delicate tissue handling

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Re the following enquiry:

I am seeking for inputs how to handle small delicate pieces of tissue during
the preparation/fixation/embedding process for TEM. There must be
something beside using fine forceps, spoons or toothpicks. What about
agar-embedding? How good is fixative penetration through agar?
Any suggestions will be welcome!


You may find the following reference of use:
B. Cribb & J. Zhu. A simple filter system for processing small or
transparent specimens. 1994. Journal of Microscopy, 173: 83-86.

We discovered that it was possible to add a TEM grid to the end of a plastic
pipette by heating the end of the pipette. This enabled us to process small
samples without loosing them or damaging them. We also refer to most of the
literature on handling small samples in the paper. I hope this is of some
use to you.

Cheers
Bronwen Cribb


--------------------------------------------------------------------------
Dr Bronwen Cribb
Centre for Microscopy and Microanalysis
& The Department of Entomology
The University of Queensland
Phone: +61-7-3365 3025 Fax: +61-7-3365 2199
--------------------------------------------------------------------------





From: Sara Miller :      saram-at-acpub.duke.edu
Date: Thu, 26 Sep 1996 18:20:43 -0400 (EDT)
Subject: Re: Tissue handling for TEM fixation/embedding

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On Thu, 26 Sep 1996, Hans-Martin Vaihinger wrote:

} Date: Thu, 26 Sep 1996 09:37:28 +0000
} From: Hans-Martin Vaihinger {Hans-Martin.Vaihinger-at-rz.ruhr-uni-bochum.de}
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: Tissue handling for TEM fixation/embedding
}
} Hello to all,
}
} I am seeking for inputs how to handle small delicate pieces of tissue during
} the preparation/fixation/embedding process for TEM. There must be
} something beside using fine forceps, spoons or toothpicks. What about
} agar-embedding? How good is fixative penetration through agar?
} Any suggestions will be welcome!
}
} Hans-Martin
}
} **************************************************************
} Hans-Martin Vaihinger
} Ruhr-University of Bochum
} Comparative Endocrinology Research Section
} Building ND 5/37
} 44780 Bochum
} GERMANY
} *********************************************************
} phone ++49 234 700 4329
} fax ++49 234 709 4551
} email Hans-Martin.Vaihinger-at-RZ.Ruhr-Uni-Bochum.de

What is it you're examining?

We use agar all the time for keeping up with cell pellets (bacterial and
tissue culture) that don't stick together by themselves. After encasing
in agar, we then handle the sample as we would a piece of tissue.

Penetration of 1% agar is fine. One secret is that you have to
glutaraldehyde-fix, THEN add the agar. If you fix the agar with the
glutaraldehyde, further fixation, dehydration, and infiltration are poor.}
After osmium fixation, the whole thing will be dark, but when excess
osmium is washed out, the cells are black, and the agar is clear so that
you can see where the sample is.

Feel free to ask if you have further questions.
Sara


Sara E. Miller, Ph. D.
P. O. Box 3020 Duke
University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: alan.wilson-at-dsto.defence.gov.au (Alan Wilson)
Date: Fri, 27 Sep 1996 12:39:53 +1000
Subject: IC Encapsulation Removal

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A colleague of mine has asked about removing integrated circuits from their
plastic moulded packaging and still having them operational. Has anyone
done this? or know how to do this? what solvents etc..

Thanks.


Alan Wilson alan.wilson-at-dsto.defence.gov.au

Senior Research Scientist
Ship Structures and Materials Division
Aeronautical and Maritime Research Laboratory
Defence Science and Technology Organization
506 Lorimer St
Fishermens Bend 3207
Victoria Australia
ph 61 3 9626 7508, fax 61 3 9626 7816 or 61 3 9626 7087






From: dan-at-bioptechs.com (Dan Focht)
Date: Thu, 26 Sep 1996 17:32:37 -0500
Subject: Re:LM live cell microscopy

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Message-Id: {v01510103ae70b4b2e8d3-at-[205.228.233.33]}
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On Thu, 26 Sep 1996 Paolo Ghiara {ghiara-at-sienanet.it} Wrote:

} Hello friends,
} I would like to study live interaction between mammalian cells and
} bacteria by time-lapsed video microscopy. Does anybody can give me a
} hint on how and where I could find details to build or buy a chamber for
} mantaining cells and bacteria alive under the microscope?
} thanks for help.
} P.Ghiara


******************************************************************
Reply

Dr.Ghiara

My company Bioptechs, develops and manufactures live cell microscopy
environmental control systems. Our systems are based around several
patented technologies.
1. Microaqueduct perfusion, used in closed system chambers to provide
compatability with all modes of microscopy, uniform temperature control and
near laminer perfusion as well as end user defined volume and flow
characteristics.
2. Objective temperature control through the use of a heating device that
attaches to the objective and is electronically controlled to within 0.2C.
(Requred when using high N.A. objectives on any chamber)
3. A closure system that requires no tools or special skill to operate,
does not leak, break coverslips or produce uneven strain on the glass
surfaces.
4. First surface thermal transport heating that provides thermal control
to the cells through a conductive coating on the bottom side of the
coverglass surface.
The thermal response of this coating technique is on the order of
0.1degree/sec. Therefore we can include a confocal setting to the
controller to eliminate the Z axis drift that occurs due to thermal
expansion.

Biotechs also provides perfusion suport for developmental and induced
change experiments on cell culture, tissue slice, and artificial membrane
experiments.

Please visit our web site for more information.

www.bioptechs.com

Dan


|\ /|
_|\\ //|_
|_|\\\~~~~~~~~~~~~~~~~~~~~~~~~///|_|
|\\\\ Daniel Focht ////|
|\\\\\_in hot water again_/////|
(____)
/ \
/______\
| HI N.A.|
| |

Bioptechs, Inc.
3560 Beck Rd.
Butler, PA 16001
Voice 412-282-7145
Fax 412-282-0745
dan-at-bioptecs.com
Live-Cell Microscopy Environmental Control web site
www.bioptechs.com






From: richard.lander-at-stonebow.otago.ac.nz (Richard Lander)
Date: Fri, 27 Sep 1996 16:50:19 +1200
Subject: Potassium ferrocyanide problem?

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To Listserv members,

We have recently been experiencing occasional difficulty with our
infiltration into various biological tissues such as Liver, Muscle etc.
In our post fixation step, we are using Potassium Ferrocyanide with OsO4 to
get enhanced contrast, and we have a vague idea that we have had
infiltration problems with potassium ferrocyanide post-fixed tissues in the
past.

Other users, not using Pot Ferro + OsO4 , have had no difficulties at all.

Has anyone else had these sort of problems with Pot Ferro before?

Look forward to hearing from you,

rich

Richard Lander
South Campus Electron Microscope Unit
c/- Pathology Department
Otago Medical School
P.O. Box 913
Dunedin
N.Z.
Tel. National 03 479 7301 Fax. National 03 479 7254

"Southernmost EM Unit in the World!"






From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Fri, 27 Sep 1996 08:36:37 +0100
Subject: Re: Contamination rates

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} Good morning all,
} Does anyone know of a reference for information on carbon contamination
} rates in microanalysis (i.e. effect of current, accelerating voltage,
} spot size etc. )? I'm going to try to do some semi-quantitative
} analyses of carbon in steel and am trying to find analytical conditions
} which minimize contamination. Also, if anyone knows of a technique(s) for
} cleaning samples before carbon analysis could they share it with me.
}
} TIA
}
} Glenn

Glen,

I've never specifically undertaken semi-quantitative C analysis in steel.
However, I am more generally fairly experienced with EM microanalysis, so a
few thoughts (apologises if I'm telling you things yoalready know),

1. I'd say that C contamination rates were almost entirely instrument
dependent, both to the specific instrumental set up and the history of
usage.

2. Specimen contamination (as opposed to the build up of a contamination
spot at the point of analysis) is probably only dependent on the level of
hydrocarbon contamination inside your vacuum and the time for which your
specimen is exposed. Electron optical conditions will not affect the build
of of contamination.

3. The development of a contamination spot at your point of analysis occurs
mainly by SURFACE diffusion of hydrocarbons across the specimen, into the
electron beam, were they are cracked down to carbon by the electron beam.
Thus those electron beam factors which maximise the interaction of the beam
with the hydrocarbons will speed up the development of the carbon spot. In
which case, lower kV and higher beam current will both increase the rate at
which the C contamination spot builds up. Adjusting the electron probe size
will (I guess) simply distribute the same volume of C over different areas.

4. Because surface diffusion is involved, the use of cryo-traps does not
directly have as much effect on specimen contamination as you might hope.
HOWEVER, cryo-trapping can help reduce the overall level of hydrocarbon
contamination in your system.

5. Knowing the above, a nice trick if you are having really big C
contamination problems is to 'flood' the specimen. That is, expose a
relatively large area of the specimen to plenty of beam current for perhaps
20-30 minutes. This cracks, and thus immobilises a very thin layer of C
contamination over the large area. The rate of surface diffusion is such
that you will get not get a C contamination spot start to build up again
for a significant period (20 minutes) of time.

6. Another approach worth trying is cryo. As with flooding, by freezing the
specimen, you immobilise the C.

7. Having said all the above, the best approach is to get the EM vacuum
system as clean as possible. With modern EMs this should not be too
difficult but with olders instruments it is a lengthy (and expensive)
process. Basically, you need to replace all oils and greases with
fluorocarbon (i.e fomblin) based materials.

Hope that helps,

Regards,

Larry

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: Larry-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
---------------------------------------------------------------






From: alan.wilson-at-dsto.defence.gov.au (Alan Wilson)
Date: Fri, 27 Sep 1996 12:39:53 +1000
Subject: IC Encapsulation Removal

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {n1368306751.13283-at-chdqm.sps.mot.com}
"Microscopy-at-Sparc5.Microscopy.Co" {Microscopy-at-sparc5.microscopy.com}
X-Mailer: Mail*Link SMTP-QM 3.0.2 GM

RE} IC Encapsulation Removal 9/27/96

We do that here on a daily basis. Generally, without knowing the exact type
of "plastic" you have, red fuming nitric acid is the etchant. First, grind a
shallow depression in the center of the top of the package (we use dremmel
tool) to create a puddle area for the acid and to ensure minimal exposure to
lead wires when etching begins. After the depression is formed, place the
package on a hot plate at 85c, then use a dropper to place a drop-at-a-time
red fuming nitric acid at 55 to 85c. When foaming stops, immediately rinse
with acetone, then repeat the droplet-rinse procedure until the die is
exposed, probably 4 to 5 iterations. If you don't have access to red fuming
nitric, white fuming will work but it will more quickly attack aluminum pads.
If you discover copper wires inside, use a 9:1 ratio of fuming nitric to
fuming sulfuric. With this mixture the cooper will remain at the expense of
the aluminum pads. In any case, metals will be attacked, it's just a matter of
getting some experience on quickly exposing only as much die as you need, then
stop.

**********************************************************
Jake Schaper
Product Analysis Lab
Advanced Digital Consumer Division
Motorola, Inc.
Chandler, Arizona
Phone 602-814-4756
**********************************************************


--------------------------------------

Thanks.


Alan Wilson alan.wilson-at-dsto.defence.gov.au

Senior Research Scientist
Ship Structures and Materials Division
Aeronautical and Maritime Research Laboratory
Defence Science and Technology Organization
506 Lorimer St
Fishermens Bend 3207
Victoria Australia
ph 61 3 9626 7508, fax 61 3 9626 7816 or 61 3 9626 7087



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From: INGRAM-at-RTI.ORG
Date: Fri, 27 Sep 1996 12:30:09 -0400 (EDT)
Subject: ADVANCES IN MICROSCOPY SYMPOSIUM

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-------------- MEETING ANNOUNCEMENT! --------------

THE NORTH CAROLINA SOCIETY FOR MICROSCOPY
AND MICROBEAM ANALYSIS

presents their

15TH. ANNUAL SYMPOSIUM ON ADVANCES IN
MICROSCOPY

*THE NEW MICROSCOPIES IN BIOLOGICAL AND
PHYSICAL SCIENCES*

Blockade Runner Resort, Wrightsville Beach
North Carolina, USA
October 25 - 27, 1996

Phone: 1 800 545 5494 *Mention Group 6665 for
special discount rates*

Contact: Peter Ingram - ingram-at-rti.org
Ann LeFurgey - ann_lefurgey-at-cellbio.duke.edu
or phone: 919 684-3534 for additional information

SYMPOSIUM SYNOPSIS

The meeting has several purposes, not the least of which
is to draw attention of the scientific community to
emerging developments in the practical and basic
research aspects of exciting new fields, and to bring
people together from diverse disciplines to discuss how
innovative techniques will be relevant to the future
direction of microscopy and microbeam analysis.
continuing with the tradition of the symposium, the guest
lecturers are composed of both nationally and
internationally distinguished scientists

The symposium also offers the opportunity for interested
participants to submit abstracts for poster display.

This year THREE SPECIAL WORKSHOPS/TUTORIALS will
be offered at NO COST to participants in the symposium.
They are:
*PRACTICAL DIGITAL IMAGING AND PRINTING*
John Mackenzie, NC State University

* CONFOCAL MICROSCOPY*
Jim Pawley, University of Wisconsin

*ENVIRONMENTAL SCANNING EM*
John Mansfield, University of Michigan

SYMPOSIUM PROGRAM

Friday October 25

4 pm Registration

6.30 pm Poolside Reception - courtesy of AMRAY

7.00 - 9.30 pm BARBECUE ON THE BEACH - courtesy of
JEOL and GATAN

Saturday October 26

8.30 - 11.30 am WORKSHOPS/TUTORIALS

POSTER SESSIONS

EXHIBITORS DISPLAYS

11.30 am NCSMMA ANNUAL BUSINESS MEETING

12 noon LUNCH

1.00 pm Welcome

1.15 pm *AFM as a Complement to SEM and TEM in
Materials Science* - BARRY CARTER

2.00 pm *What can Atomic Force Microscopy do in
Biology?* - ZHIFENG SHAO

2.45 pm *Low Voltage Lensless Microscopy in Biology
And Materials Science, and The Atom Probe*
- JOHN SPENCE

3.30 pm BREAK

4.00 pm *Z-Contrast Imaging in Materials Science*
- STEVE PENNYCOOK

4.45 pm *Z-Contrast Imaging in Biology*
- JOE WALL

5.30 pm POSTER SESSION, EXHIBITORS DISPLAYS


7.00 pm EVENING BUFFET - Al fresco, poolside
Supported by OXFORD INSTRUMENTS and LEO


Sunday October 27

8.30 am Student Awards and Presentations

9.00 am *Confocal Microscopy: Present Capabilities and
Future Directions* - JIM PAWLEY

9.45 am *Imaging Deeper, Better and Longer with
2-Photon Microscopy* - SCOTT FRASIER

10.30 am BREAK

11.00 am *Laser Microsurgery on Living Cells*
- CONLY RIEDER

11.45 am *Advances in Trace, Molecular and Isotope
Imaging using Ion Microscopy*
- RICH LINTON

12.30 pm LUNCH



SEND






From: lizard-at-okway.okstate.edu (Ginger Baker)
Date: Fri, 27 Sep 1996 13:13:52 -0700
Subject: In situ hybridization of mouse liver

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Mime-Version: 1.0

Hello all,

I have a researcher who wants to perform in situ hybridization of
DIG-labeled rRNA probes to mouse liver ultrathin sections. The
protocol is as follows: (taken from a paper by D. Fischer, D.
Weisenberger and U. Scheer in Procedures for In Situ Hybridization to
Chromosomes, Cells, and Tissue Sections: ISH to Tissues)

All steps on ice: condensed version
(1) fix in 4% paraformaldehyde in PBS, 0.5% glut plus 4%PFA in PBS.
(2) dehydrate in ETOH (30%-100% series) at -20C
(3) infiltrate and embed in Lowicryl K4M

The question is the researcher wants to use LR White, LR Gold or
Unicryl instead of Lowicryl K4M. Has anyone had any experience doing
this type of preparation? I have used the LR White, etc. but not with
the purpose of performing in situ hybridization.

Thank you for your assistance in advance,

Ginger R. Baker
EM Lab Manager

Dept. of Anatomy, Pathology, and Pharmacology
250 Veterinary Medicine
Oklahoma State University
Stillwater, OK 74078

Phone: (405) 744-6765
FAX: (405) 744-5275
Email: lizard-at-okway.okstate.edu




From: jykoo-at-erenj.com (Jay Koo)
Date: Fri, 27 Sep 1996 17:16:03 -0400
Subject: TEM Postdoc Position in Physical Metallurgy at Exxon

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Post Doctoral Postion
TEM in Physical Metallurgy
Corporate Research Laboratory, Exxon Research & Engineering Co.
Clinton Township, Annandale, NJ 08801

A post doctoral position is available immediately in the Advanced
Materials Section of the Corporate Research Laboratory, Exxon Research and
Engineering Company.

The position requires demonstrated expertise and experience in the
analytical and high resolution transmission electron microscopy
characterization of microstructures and fine precipitates in ferrous and
other alloy systems. A broad physical metallurgy background, including
structure-property relations, phase transformations, and phase stability is
also required. The person is expected to contribute to the development of
new high strength steels tailored for applications in oil and gas
industries. In a multidisciplinary and cooperative research environment,
the candidate should be able to work effectively in a team and should have
good interpersonal and communication skills.

Exxon's Corporate Research Laboratory is located in scenic, rural
western New Jersey, about an hour west of New York City and 45 minutes
northwest of Princeton. The laboratory performs basic and applied research
in support of Exxon Corporation's worldwide scientific, technological, and
business needs.

For more information, please write to us at the above address or contact:

Dr. N. Rao V. Bangaru, (Tel) 908-730-2950, (Fax) 908-730-3355

Dr. Jay Koo, (Tel) 908-730-3358, (Fax) 908-730-3355,
e-mail: jykoo-at-erenj.com

Equal Opportunity Employer M/F/H/V
Best regards,

Jay Koo
Exxon Research & Engineering Company
Annandale, NJ 08801





From: Leonard Radzilowski :      radzil-at-elt
Date: Sat, 28 Sep 1996 09:13:28 -0700 (PDT)
Subject: Cryoultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
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Dear List:

I have a question regarding cryoultramicrotomy of moisture-
sensitive polymers for TEM. My usual technique for cryo sectioning is to
collect sections with a wire loop and sucrose solution, transfer the
sections to a TEM grid, and float the grid, section-side down, on water so
as so to dissolve the remaining sucrose. I now want to section ionomers,
to which I would like to minimize exposure to water. Could anyone suggest
a method for collecting sections without water?

Thanks in advance,

Len Radzilowski

Dept. of Materials Science & Engin.
M.I.T.
Cambridge, MA 02139





From: SilverStf-at-aol.com
Date: Sat, 28 Sep 1996 15:59:34 -0400
Subject: general question

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To the listserver:

It appears that a local non-profit laboratory is about to open their doors to
everyone at very reduced rates. Their equipment was not bought using NSF
funds. Do you know of any legal [not political or ethical] reasons this
cannot be done? If so, please state statutes.

Thank you for your input.

Anne Esposito
E.M.C., e-mail: Silverstf-at-aol.com





From: smithg-at-gar.union.edu (George W. Smith)
Date: Sat, 28 Sep 1996 17:30:15 -0500
Subject: Unsubscribe

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Unsubscribe smithg-at-gar.union.edu







From: Paul.Fischione-at-internetmci.com
Date: Sun, 29 Sep 1996 18:17:21 -0500
Subject: TEM Contamination

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Message-Id: {m0v79lV-00046DC-at-fast.net}

-- [ From: Paul E. Fischione * EMC.Ver #2.3 ] --

Hello All,

To further the contamination discussion.... During the past three years I
have concentrated a great deal of effort in addressing possible sources of
TEM contamination and developing technology to reduce, if not eliminate
contamination (hydrocarbon).

We have discovered that the major source of hydrocarbon contamination is
either the specimen or the specimen holder. Some of the possible causes
are diffusion pumped or mechanical rotary backing pumped ion mills,
failure to fully remove cleaning chemicals such as acetone and alcohol from
the specimen, and adhesive residue on the specimen (popular adhesives for
attaching specimens during dimpling/tripod polishing can be hydrocarbon
based). Additionally, inadvertent touching of the specimen or specimen
holder in front of the vacuum sealing o-ring is also a major cause of
contamination.

The technology that we have found to be extremely effective is a low-energy,
, high frequency oxygen/argon plasma. The plasma is created in a vacuum
chamber which is designed to accept the specimen and the specimen holder.
The specimen holder is exposed to the plasma from the o-ring forward.
Pumping is achieved by an oil-free vacuum system in order to eliminate any
possibility of backstreaming oil from the vacuum system into the plasma
chamber. This is a critical aspect of the technology.

Ion energies are very low and the contamination is removed by means of a
chemical reduction of hydrocarbons by the oxygen radicals created within
the plasma. All of the above mentioned technology has been incorporated
into our Model 1400 Plasma Cleaner.

We have found that the vacuum systems of the newer TEM's are actually quite
clean and that the best means for high-quality microanalysis is to subject
the specimen to plasma cleaning before conducting TEM. Cleaning times are
on the order of one or two minutes for most specimens. This process results
in maintaining both microscope vacuum cleanliness and specimen integrity.
It has also been found that once a carbon spot has been created by the
electron beam it is very difficult to remove.

The effectiveness of plasma cleaning is realized in TEM's with high-
brightness guns (LaB6 and FEG). Contamination can also be a factor in
TEM's with W filament sources, however, the high current density of the FEG
often times can result in unacceptable levels of contamination when
conducting fine-probe microanalysis (EDS or PEELS).

Cryo-technology and flooding of the specimen with the electron beam are
acceptable techniques to work around the problem. It is far better to
eliminate the problem at the source.

I hope this helps.

Best regards,

Paul

Paul E. Fischione, President
E.A. Fischione Instruments, Inc.
9003 Corporate Circle
Export, PA 15632 U.S.A.
Phone (412)325-5444
FAX (412)325-5443
e-mail paul.fischione-at-internetmci.com






From: Paul.Fischione-at-internetmci.com
Date: Sun, 29 Sep 1996 18:17:21 -0500
Subject: TEM Contamination

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


-- [ From: Paul E. Fischione * EMC.Ver #2.3 ] --

Hello All,

To further the contamination discussion.... During the past three years I
have concentrated a great deal of effort in addressing possible sources of
TEM contamination and developing technology to reduce, if not eliminate
contamination (hydrocarbon).

We have discovered that the major source of hydrocarbon contamination is
either the specimen or the specimen holder. Some of the possible causes
are diffusion pumped or mechanical rotary backing pumped ion mills,
failure to fully remove cleaning chemicals such as acetone and alcohol from
the specimen, and adhesive residue on the specimen (popular adhesives for
attaching specimens during dimpling/tripod polishing can be hydrocarbon
based). Additionally, inadvertent touching of the specimen or specimen
holder in front of the vacuum sealing o-ring is also a major cause of
contamination.

The technology that we have found to be extremely effective is a low-energy,
, high frequency oxygen/argon plasma. The plasma is created in a vacuum
chamber which is designed to accept the specimen and the specimen holder.
The specimen holder is exposed to the plasma from the o-ring forward.
Pumping is achieved by an oil-free vacuum system in order to eliminate any
possibility of backstreaming oil from the vacuum system into the plasma
chamber. This is a critical aspect of the technology.

Ion energies are very low and the contamination is removed by means of a
chemical reduction of hydrocarbons by the oxygen radicals created within
the plasma. All of the above mentioned technology has been incorporated
into our Model 1400 Plasma Cleaner.

We have found that the vacuum systems of the newer TEM's are actually quite
clean and that the best means for high-quality microanalysis is to subject
the specimen to plasma cleaning before conducting TEM. Cleaning times are
on the order of one or two minutes for most specimens. This process results
in maintaining both microscope vacuum cleanliness and specimen integrity.
It has also been found that once a carbon spot has been created by the
electron beam it is very difficult to remove.

The effectiveness of plasma cleaning is realized in TEM's with high-
brightness guns (LaB6 and FEG). Contamination can also be a factor in
TEM's with W filament sources, however, the high current density of the FEG
often times can result in unacceptable levels of contamination when
conducting fine-probe microanalysis (EDS or PEELS).

Cryo-technology and flooding of the specimen with the electron beam are
acceptable techniques to work around the problem. It is far better to
eliminate the problem at the source.

I hope this helps.

Best regards,

Paul

Paul E. Fischione, President
E.A. Fischione Instruments, Inc.
9003 Corporate Circle
Export, PA 15632 U.S.A.
Phone (412)325-5444
FAX (412)325-5443
e-mail paul.fischione-at-internetmci.com






From: Gerry LITTLE :      angjl-at-medicine.newcastle.edu.au
Date: Mon, 30 Sep 1996 10:03:06 GMT +11
Subject: Re: Cryoultramicrotomy

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G'day Len,
The following reference outlines a method that we have found very
useful and easy to use.
Tsuji, S. et al., 1992, Cryoultramicrotomy: Electrostatic transfer of
dry ultrathin frozen sections grids applied to the central nervous
system, Arch. Histol. Cytol. 55: 423-428.
Regards,
Gerald Little.

Dr. Gerald J. Little,
The Neuroscience Group,
Discipline of Anatomy,
Faculty of Medicine and Health Sciences,
The University of Newcastle, Callaghan,
New South Wales, Australia, 2308.
Ph (61 49) 21 5618
Fax (61 49) 21 8667
Email ANGJL-at-Medicine.Newcastle.edu.au
Dr. Gerald J. Little,
The Neuroscience Group,
Discipline of Anatomy,
Faculty of Medicine and Health Sciences,
The University of Newcastle, Callaghan,
New South Wales, Australia, 2308.
Ph (61 49) 21 5618
Fax (61 49) 21 8667
Email ANGJL-at-Medicine.Newcastle.edu.au





From: Jim McClean :      J.McClean-at-Queens-Belfast.AC.UK
Date: Mon, 30 Sep 1996 08:37:42 PDT
Subject: Re: Cryoultramicrotomy

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unsubscribe j.mcclean-at-qub.ac.uk
===================================================================
! Jim McClean ! j.mcclean-at-qub.ac.uk !
! Head of Applications Group ! !
! Computing Services ! Tel: 01232 245133 x 3843 !
! The Queen's University of Belfast ! !
! Belfast BT7 1NN ! Fax: 01232 335066 !
===================================================================






From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Mon, 30 Sep 1996 08:23:20 +0000
Subject: Cryoultramicrotomy -Reply

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Message-Id: {s24f82da.072-at-wpo.nerc.ac.uk}
X-Mailer: Novell GroupWise 4.1

Dear Leonard

I have no experience in your particular method, but for many
years we have cut dry sections at ambient temperature of
resin embedded material for x-ray microanalysis. This
entails manipulating the sections onto carbon-coated
formvar-filmed grids with the old eyelash probe. Once on the
formvar, they can be gently tacked down with the probe,
normally at the corners and along the edges. They are then
carbon coated again for STEM work. Maybe you could try
something similar. Manual dexterity is required!

Regards - Keith Ryan





From: Microscopy-request
Date: Saturday, September 28, 1996 9:13AM
Subject: Cryoultramicrotomy

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Dear List:

I have a question regarding cryoultramicrotomy of moisture-
sensitive polymers for TEM. My usual technique for cryo sectioning is to
collect sections with a wire loop and sucrose solution, transfer the
sections to a TEM grid, and float the grid, section-side down, on water so
as so to dissolve the remaining sucrose. I now want to section ionomers,
to which I would like to minimize exposure to water. Could anyone suggest
a method for collecting sections without water?

Thanks in advance,

Len Radzilowski

Dept. of Materials Science & Engin.
M.I.T.
Cambridge, MA 02139





From: Woody.N.White-at-mcdermott.com
Date: 9/26/96 3:00 PM
Subject: dry silver printer paper

Contents Retrieved from Microscopy Listserver Archives
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A possible lead may be Alden Electronics ... Last info I have is: 53 Washington
St. Westborough, MA 01581-0500 (508) 366-8851

This info is a bit old - Hope this vendor is not the one you cannot find!

Woody White

______________________________ Reply Separator _________________________________


Hi everyone:

Does anyone know of a supplier for the special paper for an old dry silver
printer? My old vendor has vanished and I need to get a fresh supply of
paper. Thanks for any help anyone can lend.

Anne Esposito
E.M. Connection




From: Woody.N.White-at-mcdermott.com
Date: 9/26/96 9:39 PM
Subject: IC Encapsulation Removal

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I have "opened" a moulded case IC once, but it was already dead and I cannot say
if thie following process will damage the chip....

The case can be dissolved by reacting, one drop at a time, with red fuming
nitric acid near its' boiling point. Add one drop of nitric, wait until the
obvious reaction ceases, then rinse with acetone (squeeze bottle) and blow dry.
Continue until the plastic is removed.

The chip (usually SiO2 passivated) was not etched.... good luck with the wires!


______________________________ Reply Separator _________________________________


X-Sender: alan.wilson-at-SoMPop.dsto.defence.gov.au
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

A colleague of mine has asked about removing integrated circuits from their
plastic moulded packaging and still having them operational. Has anyone
done this? or know how to do this? what solvents etc..

Thanks.


Alan Wilson alan.wilson-at-dsto.defence.gov.au

Senior Research Scientist
Ship Structures and Materials Division
Aeronautical and Maritime Research Laboratory
Defence Science and Technology Organization
506 Lorimer St
Fishermens Bend 3207
Victoria Australia
ph 61 3 9626 7508, fax 61 3 9626 7816 or 61 3 9626 7087




From: slakmon-at-soquelec.com (SOQUELEC Ltd.)
Date: Mon, 30 Sep 1996 11:39:02 -0400 (EDT)
Subject: IC Encapsulation Removal

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Unsubscribe slakmon-at-soquelec.com
________________________________________________________________

Jean-Pierre Slakmon, Eng. Tel: (514) 482-6427
SOQUELEC Ltd. Fax: (514) 482-1929
5757 Cavendish Blvd., Suite 101
Montreal, Quebec e-mail: slakmon-at-soquelec.com
H4W 2W8, Canada http://www.soquelec.com
________________________________________________________________





From: Jill Craig :      jcraig-at-unbc.edu
Date: Mon, 30 Sep 1996 10:25:17 -0700 (PDT)
Subject: .tif image files for Science Week

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Hi all,

I'm looking for 5 .tif file SEM images of common objects for a contest at
the Prince George Science and Technology week. These micrographs will be
published in the paper 1 at a time for 5 days and then the kids will
guess what they are and a prize will be given to those with the most correct.

I volunteered to give the Sci and Tech week committee these micrographs.
Our machine was down at the time and now I've found out that the repair
parts will not be in until after the deadline so I can't produce them. I
know that there are lots of image pages on the web. However, since I
need free micrographs that can be published in the local paper I thought
asking in this way would be preferable.

Thank you very much for helping me out. Please attach files to e-mail as
jpg or tif and e-mail me directly. There is no money for any of us in
this, it's just for fun and education.

Thanks again, Jill




From: Chao-ying Ni; Office 312 SPL; Phone 1013 :      ni-at-me.udel.edu
Date: Mon, 30 Sep 1996 17:01:10 -0400 (EDT)
Subject: Subscription

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Hi, there. I would like to be included in the list of this news group if
possible. My e-mail address is as follows:

ni-at-me.udel.edu

Thanks very much.

Chao-Ying Ni
Materials Science Program
University of Delaware
Newark, DE 19716





From: Tom Bryner :      brynert-at-mcmaster.ca
Date: Mon, 30 Sep 1996 20:00:28 -0400 (EDT)
Subject: Hitachi S-570 SEM-film speed

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I have recently been using a Hitachi S-570 SEM. I wanted to use Polaroid 53,
but this being an older machine it does not have a setting for 800 speed
film. I was considering using the recommended setting for 400 ISO film and
close the lens aperture down one stop. Does anyone know if this is feasible
or can they recommend a better method.

Thank you
Tom Bryner





From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Mon, 30 Sep 1996 18:24:29 -0500 (CDT)
Subject: LKB Multiplate (TEM)

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Does anyone know of a vendor for a replacement heater for an LKB
Multiplate apparatus that heats wax for sealing boats to glass knives. I
put a thermometer into the one we have, and it only reads 62 C in the
wells and the same on the knife warming deck (the instructions indicate
that the temperature should be about 80 C). The wax (pink dental) is
barely melted, and we have a very short window of opportunity before the
wax solidifies. I'd appreciate any information.

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816






From: ldeg-at-midway.uchicago.edu (Linda Degenstein) (by way of Nestor J.
Date: Mon, 30 Sep 1996 18:42:27 -0500
Subject: EM Technician Opening: Part Time

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Zaluzec)


EM TECHNICAL ASSISTANT: Part Time Position

The main responsibilities of the person will include solution
preparation, sample registration, routine EM supply maintenance, processing
of biological samples for TEM, microtomy and ultramicrotomy. Some darkroom
printing of photomicrographs for analysis and data filing as well will be
required.

The person should have a college degree in biology, some knowledge of
mammalian anatomy, histology, and cell biology, with basic training, and at
least 1 year working experience in biomedical electron microscopy. The work
environment is in a very competitive research lab.

Please contact:
Dr. Q.C. Yu
The University of Chicago
5841 South Maryland
MC 1028 Rm. N350
Chicago, Illinois 60637

Please include your resume, a letter of intent, and a list of 3
references. The University of Chicago is an Equal Opportunity Employer.


Sincerely,
Linda Degenstein
ldeg-at-midway.uchicago.edu






From: Heather A Owen
Date: 01 October 1996 05:00
Subject: LKB Multiplate (TEM)

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owenha-at-csd.uwm.edu (Heather A Owen)
Message-ID: {1996Oct01.101559.1814.79639-at-missgate.sunderland.ac.uk}
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Heather

my knowledge of the workings of our LKB multiplate are extremely limited but
I would suspect, if you are getting heat but not enough, that there may be a
faulty thermostat rather than heater. Sorry I don't know where to get one of
those either.

Malcolm Haswell
E.M. Unit
University of Sunderland
U.K.

----------


Does anyone know of a vendor for a replacement heater for an LKB
Multiplate apparatus that heats wax for sealing boats to glass knives. I
put a thermometer into the one we have, and it only reads 62 C in the
wells and the same on the knife warming deck (the instructions indicate
that the temperature should be about 80 C). The wax (pink dental) is
barely melted, and we have a very short window of opportunity before the
wax solidifies. I'd appreciate any information.

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816






From: Probing & Structure :      p&s-at-ultra.net.au
Date: Tue, 01 Oct 1996 23:10:04 +1000
Subject: Re: SEM-film speed & aperture

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At 20:00 30/09/96 -0400, you wrote:
} I have recently been using a Hitachi S-570 SEM. I wanted to use Polaroid 53,
} but this being an older machine it does not have a setting for 800 speed
} film. I was considering using the recommended setting for 400 ISO film and
} close the lens aperture down one stop. Does anyone know if this is feasible
} or can they recommend a better method.
}
} Thank you
} Tom Bryner
******************************
Hi Tom and whoever:
I believe that you cannot do better than to close down the camera
by one stop. The only other consideration is lens resolution. For that size
format best resolution for most lenses would be between f5.6 to 11. Lenses
designed for 35mm format are likely to have best resolution with the
aperture one stop more open.
If the image is properly in focous, the additional depths of field
is no consideration when photgraphing a flat screen.
Cheers
Jim Darley
Probing & Structure
} (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia
}
} Phone +61 77 740 370 Fax: +61 77 892 313
} A great microscopy site http://www.ultra.net.au/~pns/





From: akracher-at-iastate.edu (Alfred Kracher)
Date: Tue, 1 Oct 1996 09:22:01 -0600
Subject: EPMA on Mn in sulfides etc.

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What do people who microprobe sulfides and other ore samples use as Mn
standard? In my experience pure Mn metal corrodes too fast to be useful.

-----------------------------------------
Alfred Kracher
akracher-at-iastate.edu
http://www.public.iastate.edu/~akracher
-----------------------------------------






From: roy-at-bayou.uh.edu (Roy Christoffersen)
Date: Tue, 01 Oct 1996 10:04:53 -0500
Subject: Re:"White atoms": O'Keefe was right

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A belated thank you to the few of you who replied to my posting about
persistent white atoms in HRTEM simulations. It would appear that Mike
O'Keefe hit the nail squarely, since the white atoms disappear when I add
16 milliradians of crystal tilt to the simulation. They are also not
present for very small specimen thicknesses.

Also, thanks to Larry Allard I will spell "Scherzer" correctly for the rest
of my life.....


Roy Christoffersen
Texas Center for Superconductivity
3201 Cullen
Houston, TX 77204-5932
roy-at-bayou.uh.edu
(713) 743-8273
FAX: (713) 743-2787






From: Warren Straszheim :      wes-at-ameslab.gov
Date: Tue, 01 Oct 1996 09:20:45 -0500
Subject: Re: Hitachi S-570 SEM-film speed

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Message-ID: {n1367960291.9654-at-msgw.mayo.edu}

Should be. We do it often with our JEOL 840. We normally run 100 seconds at
f-5.6 for Type 55 film. If I am in a hurry, I will shorten the exposure to
50 seconds and open the aperture to f-4. Each f-stop change will double or
halve the light coming through, so just expose it half or twice as long,
respectively.

At 08:00 PM 9/30/96 -0400, you wrote:
} I have recently been using a Hitachi S-570 SEM. I wanted to use Polaroid 53,
} but this being an older machine it does not have a setting for 800 speed
} film. I was considering using the recommended setting for 400 ISO film and
} close the lens aperture down one stop. Does anyone know if this is feasible
} or can they recommend a better method.
}
} Thank you
} Tom Bryner
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Chao-ying Ni; Office 312 SPL; Phone 1013 :      ni-at-me.udel.edu
Date: Tue, 1 Oct 1996 13:35:00 -0400 (EDT)
Subject: subscribe

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From: A. Greene :      ablue-at-mail.io.com
Date: Tue, 1 Oct 1996 13:02:32 -0500 (CDT)
Subject: Re: Hitachi S-570 SEM-film speed

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Message-Id: {m0v88Yv-0003MYC-at-fast.net}

Hello Tom, Yours is an excellent solution since one larger f-stop number
(not larger opening) is about the same as one/half the light or twice the
film speed. Besides, your depth of field and therefore , focus may be better.

Good luck. Alex Greene
Scientific Instrumentation Services, Inc.
[Independent Electon Microscope Service]
Number 499, Post Office Box 19400
Austin, Texas 78760




At 08:00 PM 9/30/96 -0400, you wrote:
} I have recently been using a Hitachi S-570 SEM. I wanted to use Polaroid 53,
} but this being an older machine it does not have a setting for 800 speed
} film. I was considering using the recommended setting for 400 ISO film and
} close the lens aperture down one stop. Does anyone know if this is feasible
} or can they recommend a better method.
}
} Thank you
} Tom Bryner
}
}
}





From: luciom-at-NEWTON.UMSL.EDU (Luciano Mule'Stagno)
Date: Mon, 30 Sep 1996 15:59:23 -0500
Subject: TEM specimen prep. - silicon on insulator.

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Hi All,
we have been trying to find different ways to prepare SOI specimens
for TEM. The material of interest is a silicon wafer which has an oxide lare
on it, on top of which there is the silicon (SOI) layer. This layer can be
as thin as 0.1-0.2microns, and the oxide can be 0.1-0.8 microns. It would be
nice if we could lift off the top layer only as a TEM specimen. I have tried
placing in an HF bath, however it takes sufficiently long to get thorough
all the oxide, that you start seeing hjoles in the top layer too. I was
trying to figure out a way to coat the top layer with something robust and
non-damaging and inert (or reacts slowly with HF), and which could be easily
removed after lift-off. I thought of super-glue or wax, but thought I'd ask
if anyone has had similar experiences before I try it out.

Any suggestions?

cheers

Lucio


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr.Lucio Mule'Stagno
MEMC Electronic Materials Inc University of Missouri -St.Louis
Silicon Materials Research Group Physics Dept.,
501 Pearl Dr., 8001 Natural Bridge rd.,
St.Peters, St.Louis
MO 63376 MO 63121
tel: 314 279 5338 314 516 5931/3
fax: 314 279 5363 314 516 6152
email: lmulestagno-at-memc.com luciom-at-newton.umsl.edu
URL:http://www.newton.umsl.edu
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From: Dave King (607)757-1248 T37/257-3 Ext deking-at-vnet.ibm.com
Date: 1 Oct 1996 10:33:46 EDT
Subject: Hitachi S-570 SEM-film speed

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Message-Id: {199610011530.KAA18895-at-Sparc5.Microscopy.Com}

To: Tom Bryner {brynert-at-mcmaster.ca}
Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}
*** Reply to note of 10/01/96 08:51

Tom,

What you suggest for setup is fine, but you need to go further.

Most SEM's have a "graph" mode that shows the video signal
intensity across a line on the image. You need to mark the CRT
bezel (or similar reference) for where the image signal is barely
white on the top, and barely black on the bottom.

A high contrast sample with full grey scale range works nicely.
Trial and error until you get an OK photo, and then note areas
where you just barely maintained highlight detail and one where
you just barely maintained shadow detail. Look at the graph in
these areas and mark the levels on the bezel. This has to all be
done with no changes in the setup, and the beam must be stable.
If your getting level drifts, you'll have to settle it down 1st.
After that, you're set with very exact repeatable reference marks
to go by. Try it, and let me know how you do.

. {
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: H. ADAMS :      hadams-at-nmsu.edu
Date: Tue, 1 Oct 1996 18:19:03 -0600 (MDT)
Subject: EM lab support

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Message-ID: {n1367923537.70881-at-qmgate.anl.gov}

I was asked by our very recently appointed User's Committee of the EM
core facility of the university which has been given the task "to
explore options for the future of the lab" to find out how other similar
university facilities are supported. In particular, how much support is
provided by the central administration, the colleges and/ or departments
for the running of the lab? Also, where do such labs usually sit in the
infrastructure of the university, ie. in a college,underthe vp for
research or other entity? The university,I guess is a midsize
institution of around 15,000 so I believe a core type facility makes
sense in reducing redundancy, costs, etc., but so far the users and myself
are having a hard time convincing the central administration this.
Thanks in advance
Hank Adams
EML
New Mexico State University




From: geoffa-at-amsg.austmus.gov.au (GeoffA)
Date: 30/9/96 8:00 PM
Subject: Hitachi S-570 SEM-film speed

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Hi Tom,

Closing the aperture by one stop is the simplest solution.

Another method you might consider (about which I'd welcome comments from
the EM community) is to recalibrate the output levels of the record screen
amplifier to match the faster film. The benefits are;

1) you will know for sure that the output matches the film rather than
relying on "what should be the case" (and possibly being thwarted by
unforseen irregularities in the system). Also, I gather that the phosphor
in the screens "ages", so if it's been a long while since you've matched
output to film, you should probably do this anyway.

2) The width of the scan line of the record screen is related to the
brightness. I assume this is due to bright phosphor particles exciting
neighbouring particles. Excessive brightness could ultimately, I expect,
produce overlapping scan lines, reducing the effective resolution of the
record screen image - this appeared to be the case in our SEM. (Note: you
can also lose resolution if the electron beam in the CRT is not properly
focussed - this is adjustable on our SEM). To cut a long story short, I
opted to match the film-speed to my chosen output level of the record
screen (maintaining optimal f-stop) rather than the normal way of matching
output to film/camera.

In your case, retuning the output to a faster film would entail reducing
the brightness. This would be going in the right direction for reducing
the variation in width of the scan line on the record CRT.

I'd welcome comments on this from others, particularly if it introduces
other detrimental effects of which I'm not currently aware.

Cheers, Geoff

::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
Geoff Avern
Manager
Microscopy Laboratories
Australian Museum Email: geoffa-at-amsg.austmus.oz.au
6 College St Ph: (61)(2) 9320 6198
Sydney, Australia. 2000 Fax: (61)(2) 9320 6059
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::







______________________________ Reply Separator _________________________________


I have recently been using a Hitachi S-570 SEM. I wanted to use Polaroid 53,
but this being an older machine it does not have a setting for 800 speed
film. I was considering using the recommended setting for 400 ISO film and
close the lens aperture down one stop. Does anyone know if this is feasible
or can they recommend a better method.

Thank you
Tom Bryner





From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Wed, 2 Oct 1996 13:22:30 +1000 (EST)
Subject: Focussed Ion Beam Milling

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On a recent visit to Hitachi Naka Works I was shown a Focussed Ion Beam
Miller (the FB2000). They are commonly used in semiconductor defect
analysis. Does any one know who else makes them?

Thanks,

Mel Dickson
E.M. Unit,
UNSW, Sydney, Australia





From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Tue, 1 Oct 1996 20:12:47 -0800
Subject: Re: Hitachi S-570 SEM-film speed

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Dear Tom,
If you can stand another message on your S-570, here's mine. I also have an
S-570. Yes, closing the f-stop one stop should adjust the camera for the
800 ASA film. Also, if you need to adjust the camera slightly, there are
adjustments for photo CRT brightness and contrast on the panel just to the
left of the camera, under the cover. If you run out of adjustment, there
are coarser adjustments in the back that the Hitachi service people can
tweak.
Hope this helps,
Regards,
Mary

Tom Bryner wrote:
} I have recently been using a Hitachi S-570 SEM. I wanted to use Polaroid 53,
} but this being an older machine it does not have a setting for 800 speed
} film. I was considering using the recommended setting for 400 ISO film and
} close the lens aperture down one stop. Does anyone know if this is feasible
} or can they recommend a better method.
}
} Thank you
} Tom Bryner

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Tue, 1 Oct 1996 20:20:19 -0800
Subject: Re: Video capture

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Dear Eugene:
The definition of "video" is the 640 X 480 size. You must use a higher
resolution camera to get a higher resolution image. These, however, will
not be compatible with a video VHS recorder and will not work at real time
speeds.

Eugene Krueger wrote:
} The lab I'm in uses time lapse recorders (Super VHS) for videoing live cells
} on a microscope stage under different conditions. The resulting videotape
} looks terrific on a TV/ monitor. The problem is when I videocapture from the
} tape (for posters or papers), the resulting image is limited to 640 x 480
} pixels even if I set my monitor as high as 1024 x 768. Is this a limitation
} of the software/hardware set-up I'm using, or is this the limit of resolution
} of VHS/ SVHS tapes? I perform the captures on a PowerMac 8500 using a SVHS
} recorder and the built-in SVHS port of the mac. The capture software is Apple
} Video Player (free with the mac). Would a commercial software product allow
} me to capture "larger" images? Would a different videoboard matter?
}
Luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Probing & Structure :      p&s-at-ultra.net.au
Date: Wed, 02 Oct 1996 14:26:09 +1000
Subject: Re: LKB Multiplate (TEM)

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At 18:24 30/09/96 -0500, you wrote:
}
} Does anyone know of a vendor for a replacement heater for an LKB
} Multiplate apparatus that heats wax for sealing boats to glass knives. I
} put a thermometer into the one we have, and it only reads 62 C in the
} wells and the same on the knife warming deck (the instructions indicate
} that the temperature should be about 80 C). The wax (pink dental) is
} barely melted, and we have a very short window of opportunity before the
} wax solidifies. I'd appreciate any information.
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816
*************************************************
Hello Heather and other multiplaters:
LKB was swallowed up by Leica and they should have spare parts.
The gadget does not have a thermostat but relies on the heater to reach a
design maximum which is a bit over 70 degrees. This could be limited by low
ambient temperature, poor heat transfer from the element and wrong operating
voltage. If the element is designed for 120 volts but the line voltage is
only 110, considerably lower temperatures will result. Your electronic
technicians will have some heat transfer paste. Smearing some of that over
the cylinder shaped element may do the trick.
I prefer a small adjustable lab hot plate with an Al gadget on top to hold
wax and spatula. I prefer to seal glass boats with dental wax rather than
nail varnish; I think that poor temperature control of the dental wax is the
main reason that varnish is used.
Cheers
Jim Darley
Probing & Structure
} (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia
}
} Phone +61 77 740 370 Fax: +61 77 892 313
} A great microscopy site http://www.ultra.net.au/~pns/





From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Tue, 1 Oct 1996 20:50:32 -0800
Subject: Re: Contamination rates

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Dear Glenn,
The best reference I know is Bastin et al in the MAS Conference Proceedings
of five or six years ago. He did a very thorough study of light element
analysis spanning several years and including a study of contamination,
since it is so important in light element analysis. He also attempted to
work out a method of analysing the C in steel by graphing the apparent C
level and extrapolating back to time = 0. He also found that most
contamination is specimen derived. I attempted some C in steel several
years ago and on "good" days it was almost possible. Use low kV and as low
a current as you can to get the x-rays. I then attempted to study the
contamination rates on a piece of polished oxygen-free copper and found
that it varied widely and without any logical reason. One source I found
was if the e-beam hit a bit of mounting epoxy, the contamination level was
high for three days after. I attempted to use an air-jet that injected air
or oxygen on to the sample to remove contamination or prevent its deposit,
but it only worked once in five days of trying, and I don't know why. One
thing I tried for the C in steel was to put the sample into the camber the
evening before the analysis, so it pumped overnight and was not vented in
the morning before the analysis was done.
It is always a problem. Hope this helps
Regards,
Mary

Glenn Poirier wrote:
} Does anyone know of a reference for information on carbon contamination
} rates in microanalysis (i.e. effect of current, accelerating voltage,
} spot size etc. )? I'm going to try to do some semi-quantitative
} analyses of carbon in steel and am trying to find analytical conditions
} which minimize contamination. Also, if anyone knows of a technique(s) for
} cleaning samples before carbon analysis could they share it with me.
}
} TIA
}
} Glenn

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Jorge Canchaya :      inquil-at-amauta.rcp.net.pe
Date: Wed, 02 Oct 1996 05:26:19 -0500
Subject: Video system for microscopes

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Message-ID: {3252434B.1B86-at-amauta.rcp.net.pe}

Hi:

Could anybody out there help me? I am looking for an imaging video
system which can digitalize what is observed in a microscope, and send
to a screen (computer of TV set). I will really appreciate your opinions
about the best ones.

Thank you in advance

-------------------------
Carlos A. Canchaya
Genetic Resources and Plant Biotechnology Research Center
National Agrarian University
Lima-Peru
inquil-at-amauta.rcp.net.pe




From: Self :      RISRMS1/AFM-JQBI
Date: Wed, 2 Oct 1996 12:47:04 +0200
Subject: Re: contamination rates

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------- Forwarded Message Follows -------

Dear Glenn,

In addition to the remarks by Mary Mager:
G. F. Bastian and H. J. M. Heijligers from Laboratory for Physical
Chemistry, University of Technology, Eindhoven, Netherlands have
written a detailed report on the matter. The title is "Quantitative
electron probe microanalysis of carbon in binary carbides" and its
ISBN number is 90-6819-004-0 CIP.

Regards,
Joergen.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
J. B. Bilde-Soerensen
Materials Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 35 11 73




From: Jorge Canchaya :      inquil-at-amauta.rcp.net.pe
Date: Wed, 02 Oct 1996 05:26:09 -0500
Subject: Universal Microscope (Aus Jenna). Optical-microscope information. Alternatives

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Message-ID: {32524341.C55-at-amauta.rcp.net.pe}

Hi:

I would like to contact with people who knows or is using the Universal
Microscope of Aus Jenna. We have one in our lab and it have most of his
parts and accesories but we do not know how to assemble it. There is no
representant of Aus Jenna in my country. We do not also have its model
and date of fabrication. It seems that it is a very good microscope for
research. My questions are:

- Does anybody know how to obtain its handbook or manual of its parts
and how to use it?
- Do you know information about what the model is and when it was
fabricated?
- Does Aus Jenna have a Web site in Internet?
- Could anyone suggest the best alternative for that microscope, does
the alternative have
all the same features of the Universal (fluorescence,
phase-constrast,inverted microscopy etc)


We really appreciate your future help.

Thank you in advance,

-------------------------
Carlos A. Canchaya
Genetic Resources and Plant Biotechnology Research Center
National Agrarian University
Lima-Peru
inquil-at-amauta.rcp.net.pe




From: Nuria Cortadellas :      nuriac-at-giga.sct.ub.es
Date: Wed, 2 Oct 1996 15:37:32 +0000 (GMT)
Subject: Molds

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Does anyone knows some Beem flat embedding molds from polyethylene deeper
than 3mm? we have some problems with embedding media such as nanoplast,
lowicryls or LR-white...because the bloc is too thin and sometimes it
breaks.
Thanks in advanced
Nuria Cortadellas





From: csedax-at-alpha.arcride.edu.ar
Date: Wed, 2 Oct 1996 12:59:56 -2036
Subject: thanks

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Hi all!

Thanks for answer my question refered to the tensile and torsion test by SEM,
what you send me, was an important help to complete my thesis, thanks again

Daniel F. Imbert




From: Crossman, Harold :      crossman-at-rd.sylvania.com
Date: Wed, 2 Oct 1996 09:47:00 -0400
Subject: image capture

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Message-Id: {c=US%a=_%p=SYLVANIA%l=SYLVANIA/OSI/00039ADA-at-da-exc1.sylvania.com}
microscopy
{Microscopy-at-Sparc5.Microscopy.Com}

Sir,

If you have access to the world wide web, a great place to start it the
NIH Image home page: http://rsb.info.nih.gov/nih-image/
NIH Image is image processing, acquisition share-ware that works on the
Mac. There will soon be Windows (95?) version as well. There are
thousands of users around the world and many derivatives of the program
and wide commercial support. There is also a very active NIH Image
listserver similar to this one.


We use several frame grabber boards. My favorites:
Data Translation Quick Capture for Mac (from video source) , and Data
Translation 3152 for PCI-bus Win 3.1(from analog & digital SEM signals)
We have all but eliminated the use of Polaroid film in our lab with
savings of many thousands of dollars (US) per year.


-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.osram.de
www.siemens.com




From: Woody.N.White-at-mcdermott.com
Date: 9/30/96 12:25 PM
Subject: .tif image files for Science Week

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For a few assorted SEM images, check my homepage at

http://www.geocities.com/capecanaveral/3722

Woody White


______________________________ Reply Separator _________________________________


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Hi all,

I'm looking for 5 .tif file SEM images of common objects for a contest at
the Prince George Science and Technology week. These micrographs will be
published in the paper 1 at a time for 5 days and then the kids will
guess what they are and a prize will be given to those with the most correct.

I volunteered to give the Sci and Tech week committee these micrographs.
Our machine was down at the time and now I've found out that the repair
parts will not be in until after the deadline so I can't produce them. I
know that there are lots of image pages on the web. However, since I
need free micrographs that can be published in the local paper I thought
asking in this way would be preferable.

Thank you very much for helping me out. Please attach files to e-mail as
jpg or tif and e-mail me directly. There is no money for any of us in
this, it's just for fun and education.

Thanks again, Jill




From: Paolo Ghiara :      ghiara-at-sienanet.it
Date: Wed, 02 Oct 1996 19:04:08 -0700
Subject: Re: Video system for microscopes

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Message-Id: {32531F18.5123-at-sienanet.it}

Jorge Canchaya wrote:
}
} Hi:
}
} Could anybody out there help me? I am looking for an imaging video
} system which can digitalize what is observed in a microscope, and send
} to a screen (computer of TV set). I will really appreciate your opinions
} about the best ones.
}
} Thank you in advance
}
} -------------------------
} Carlos A. Canchaya
} Genetic Resources and Plant Biotechnology Research Center
} National Agrarian University
} Lima-Peru
} inquil-at-amauta.rcp.net.pe

Hi,
It depends on what you want to look at. If you are interested in
histopathology (as I do) and make image archiving and analysis, you
would need a high resolution color camera (3CCD or, better, a digital
camera). for the CCD technology you would need a framegrabber (Matrox
has a wide range of offer), for the digital camera you need a SCSI port
on the PC. the final output will be an image file that is small or huge
(for usual CCD cameras and framegrabbers is 1-2MB, for the digital
camera you usually have much bigger files). An important item is the PC:
try to get a Pentium PC (PCI structure) or a PowerMac. Then you can
download the image analysis software from NIH for free or buy a more
sophisticated program. Another important thing is the video monitor: I
think the best is a SONY Trinitron.
If you are interested in time-lapsed live video microscopy a B/W CCD
camera is sufficient and the cost is more affordable, but you may need a
more sophisticated hardware for image storage and retrieve (i.e. speedy
optical disks and software for movie reconstruction).
In conclusion you may need to pay anything between 8,000 and 60,000 USD
for a decent system. Is up to you.

best wishes

Paolo Ghiara
Dept of Immunology - Chiron Biocine SpA, Italy
ghiara-at-sienanet.it




From: Joe D Geller :      geller-at-world.std.com
Date: Wed, 2 Oct 1996 07:51:27 -0400 (EDT)
Subject: Re: SEM-film speed & aperture

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On Tue, 1 Oct 1996, Probing & Structure wrote:

} At 20:00 30/09/96 -0400, you wrote:
} } I have recently been using a Hitachi S-570 SEM. I wanted to use Polaroid 53,
} } but this being an older machine it does not have a setting for 800 speed
} } film. I was considering using the recommended setting for 400 ISO film and
} } close the lens aperture down one stop. Does anyone know if this is feasible
} } or can they recommend a better method.
} }
} } Thank you
} } Tom Bryner
} ******************************
} Hi Tom and whoever:
} I believe that you cannot do better than to close down the camera
} by one stop. The only other consideration is lens resolution. For that size
} format best resolution for most lenses would be between f5.6 to 11. Lenses
} designed for 35mm format are likely to have best resolution with the
} aperture one stop more open.
} If the image is properly in focous, the additional depths of field
} is no consideration when photgraphing a flat screen.
} Cheers
} Jim Darley
} Probing & Structure
} } (Microscopy Supplies & Accessories)
} PO Box 111, Thuringowa QLD 4817 Australia
} }
} } Phone +61 77 740 370 Fax: +61 77 892 313
} } A great microscopy site http://www.ultra.net.au/~pns/
}
Years ago I did a study on the appropriate F stop to set the camera on the
image recording unit for the SEM (JEOL-35). That instrument had a F4.5
lens and the ability to adjust the contrast and brightness independently.

The end result was interesting. The quality parameter measured was the
number of line pairs resolved on the photographic image (Polaroid type 55
was used since it has the resolution to see 2000 line pairs over the 4X5"
image. The best result was F8. At smaller F stopsl (F11 and lower) the
brigtness had to be increased which had the result of blooming- the
phosphor was being pushed too hard. At larger F stops there was a lack of
depth of field field resulting in the outer corners of the image being out
of focus. Also noticed was defocusing of the image due to "mottling" of
the negative material.

Incidentally, our MRS-3 standard has patterns to help check CRT
resolution.


Hope this little tutorial helps.

Joe Geller
Geller Microanalytical Lab
Topsfield, MA 01983
www.gellermicro.com





From: kris-at-elod.vein.hu (Kris Kovacs)
Date: Wed, 2 Oct 1996 19:49:39 +0100
Subject: Universal Microscope (Aus Jenna). Optical-microscope information. Alternatives

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Dear Carlos:

} - Does Aus Jenna have a Web site in Internet?

I cannot help you in finding the data you are looking for, but I must
comment some facts. The microscope in question is obiously German made, the
city where the factory is located is JENA (not Jenna), and "aus" means
"from" in German, so you should look at this as "an universal microscope
from Jena". By the way, Jena is the home city of Carl Zeiss Jena,
geographically located in the Eastern part of Germany. Hope someone can help
you more in this matter, and maybe if you are looking either for "Zeiss" or
for "Jena" on the Web you might find something useful.
Good luck!
Kris


*************************************************************************
Kristof KOVACS
Associate Professor
University of Veszprem, Central Laboratory
P.O.Box 158, Veszprem, HUNGARY
H-8201
Phone: +36-(88)-421-684
*************************************************************************





From: Microscopy-request
Date: Monday, September 30, 1996 3:59PM
Subject: TEM specimen prep. - silicon on insulator.

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Hi All,
we have been trying to find different ways to prepare SOI specimens
for TEM. The material of interest is a silicon wafer which has an oxide lare
on it, on top of which there is the silicon (SOI) layer. This layer can be
as thin as 0.1-0.2microns, and the oxide can be 0.1-0.8 microns. It would be
nice if we could lift off the top layer only as a TEM specimen. I have tried
placing in an HF bath, however it takes sufficiently long to get thorough
all the oxide, that you start seeing hjoles in the top layer too. I was
trying to figure out a way to coat the top layer with something robust and
non-damaging and inert (or reacts slowly with HF), and which could be easily
removed after lift-off. I thought of super-glue or wax, but thought I'd ask
if anyone has had similar experiences before I try it out.

Any suggestions?

cheers

Lucio


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr.Lucio Mule'Stagno
MEMC Electronic Materials Inc University of Missouri -St.Louis
Silicon Materials Research Group Physics Dept.,
501 Pearl Dr., 8001 Natural Bridge rd.,
St.Peters, St.Louis
MO 63376 MO 63121
tel: 314 279 5338 314 516 5931/3
fax: 314 279 5363 314 516 6152
email: lmulestagno-at-memc.com luciom-at-newton.umsl.edu
URL:http://www.newton.umsl.edu
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From: houpt-at-worldxs.worldaccess.nl (houpt)
Date: Wed, 2 Oct 1996 21:47:29 +0100
Subject: Universal Microscope (Aus Jenna). Optical-microscope information. Alternatives

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Message-Id: {199610021943.VAA16729-at-worldxs.worldaccess.nl}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Hi:

I would like to contact with people who knows or is using the Universal
Microscope of Aus Jenna. We have one in our lab and it have most of his
parts and accesories but we do not know how to assemble it. There is no
representant of Aus Jenna in my country. We do not also have its model
and date of fabrication. It seems that it is a very good microscope for
research. My questions are:

- Does anybody know how to obtain its handbook or manual of its parts
and how to use it?
- Do you know information about what the model is and when it was
fabricated?
- Does Aus Jenna have a Web site in Internet?
- Could anyone suggest the best alternative for that microscope, does
the alternative have
all the same features of the Universal (fluorescence,
phase-constrast,inverted microscopy etc)


We really appreciate your future help.

Thank you in advance,

-------------------------
Carlos A. Canchaya
Genetic Resources and Plant Biotechnology Research Center
National Agrarian University
Lima-Peru
inquil-at-amauta.rcp.net.pe


BIOMET:Applied Phycological Research
and biological microscopy consultancy.






From: Michael J. Lyon :      lyonm-at-vax.cs.hscsyr.edu
Date: Wed, 02 Oct 1996 17:03:29 -0400
Subject: Staining compatibility

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I am looking for some help. I am trying to dissect out some very small
{50 um blood vessels. The main problem is that I cannot see them once
the blood has washed out. I would like to stain the vessels with
something that is compatible with immunohistochemical procedures using
confocal microscopy. I am concerned that the vessel staining will
interfere with the fluorescence when viewed on the confocal either by
scattering or autofluorescence. Since alkaline phosphatase is present
in most vessels and is still active after paraformaldehyde fixation,
this should would work for the vessel stain. I am using rats and most
likely the method of Mayahara et al., Histochemie 11:88;1967. Any
advice would be appreciated.

Thanks

Mike




From: SilverStf-at-aol.com
Date: Wed, 2 Oct 1996 17:08:41 -0400
Subject: follow up info. on gen. question

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To the listserver:

In regards to my previous request for information regarding a non-profit lab c
ontemplating going into competitiion with commercial labs, I would like to
thank those that responded to me with their comments. Here are some
additional details that answer your questions.

The location should not matter. They want to do it to survive so that they
can get their own work done. They'll probably only need about 25% from
outside sources, so it is believed....They would not turn a profit since any
work would be done at cost. Their current equipment inventory does not
include any federally funded instruments. We cannot find any laws that
address the unfair competition possibility. They do not see it any different
than a bookstore at a school being in competition with commercial bookstores.
The activity has not yet started but is likely to unless we find some legal
objections.

Any further input would be appreciated.

Anne Esposito
E.M.C., e-mail: Silverstf-at-aol.com








From: gerry_nas-at-antdiv.gov.au (Gerry Nash)
Date: Thu, 3 Oct 1996 08:45:09 +1000
Subject: Penguin Eyeballs

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G'day from Oz,
Please would any of you wonderful microscopists out there have looked at
Penguin Eyeballs under LM or EM. I'm particularly interested in the retina
structure under SEM or TEM.
Thank you
Gerry

Ms Geraldine Nash
Electron Microscopist

EM Unit
Australian Antarctic Division
Channel Highway
Kingston
Tasmania 7050
Australia Ph: + 61 03 62 323 322 Fax: + 61 03 62 323 351






From: Henrik Kaker :      Henrik.Kaker-at-guest.arnes.si
Date: Thu, 03 Oct 1996 00:53:04 +0100
Subject: Re: Universal Microscope (aus Jena)

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For Carl Zeiss WWW address please see our database at:

http://www2.arnes.si/guest/sgszmera1/vendors.html


--
Henrik Kaker
SEM-EDS Laboratory, Metal Ravne d.o.o., Slovenia,
Tel: +386 602 21 131 Fax: +386 602 20 436
Henrik.Kaker-at-guest.arnes.si,
http://www2.arnes.si/guest/sgszmera1/index.html
Microscopy Vendors Database:
http://www2.arnes.si/guest/sgszmera1/vendors.html




From: Henrik Kaker :      Henrik.Kaker-at-guest.arnes.si
Date: Thu, 03 Oct 1996 00:53:04 +0100
Subject: Re: Universal Microscope (aus Jena)

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For Carl Zeiss WWW address please see our database at:

http://www2.arnes.si/guest/sgszmera1/vendors.html


--
Henrik Kaker
SEM-EDS Laboratory, Metal Ravne d.o.o., Slovenia,
Tel: +386 602 21 131 Fax: +386 602 20 436
Henrik.Kaker-at-guest.arnes.si,
http://www2.arnes.si/guest/sgszmera1/index.html
Microscopy Vendors Database:
http://www2.arnes.si/guest/sgszmera1/vendors.html




From: JOSEPH COHEN :      JDCOHEN-at-stoel.com
Date: Wed, 02 Oct 1996 15:51:51 -0700
Subject: Re: Focussed Ion Beam Milling -Reply

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Message-Id: {s2528d9d.027-at-wpsmtp.stoel.com}
X-Mailer: Novell GroupWise 4.1

1. FEI's current address and phone numbers are:

FEI Company
7451 NE Evergreen Parkway
Hillsboro, OR 97124-5830
USA
voice: (503) 640-7500
fax: (503) 640-7509

Good Contacts there would be Phil Fischer and Brent Townsend.
There are also European contacts:
FEI Europe Ltd. Cambridge, UK
44-0954-250-526

FEI Europe, GmbH, Munich, Germany
49-894-623-450

A recent FEI technical newsletter ("FEI focus") says that they'll be at the
following trade shows through the end of this year:
American Vacuum Soc., Oct. 15-17, Philadelphia, Pennsylvania, US
Semicon/Southwest, Oct 15-16, Austin, Texas, US
ISTFA '96, Nov 18-20, Los Angeles, California, US
MRS, December 2-5, Boston, Massachusetts, US
Semicon/Japan, Dec. 4-6, Makuhari, Japan

If the machine that Mel Dickson saw was a "FIB 200," it was an FEI model.

FEI also makes systems ("DualBeam") that combine an FIB system with an
SEM.


} } } Scott D. Walck WL/MLBT {walcksd-at-ml.wpafb.af.mil} 10/02/96
06:44 } } }
} On a recent visit to Hitachi Naka Works I was shown a Focussed Ion
Beam
} Miller (the FB2000). They are commonly used in semiconductor defect
} analysis. Does any one know who else makes them?
}
} Thanks,
}
} Mel Dickson
} E.M. Unit,
} UNSW, Sydney, Australia
}
There's two in USA:

Micrion 1 Corporation Way
Peabody, MA, 01960 USA
(508) 531-6464


FEI
19500 NW Gibbs Dr., Suite 100
Beaverton. OR 97006 USA
(503)690-1500

For your information, there will be two talks on FIB sample preparation at
the
TEM Sample Preparation Symposium Z at the Spring '97 Materials
Research Society meeting in San Francisco.

- -Scott Walck






From: r.g.white-at-sci.monash.edu.au (Rosemary White)
Date: Thu, 03 Oct 1996 12:18:33 +1200
Subject: Re: Video capture

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Dear Eugene,

We've just started capturing images directly to the powermac using the
built in frame grabber, and not a problem - you can set the resolution to
whatever the computer is capable of - for single images, we find a canvas
size of 1024x768 and millions of colours is fine, and so far have used only
the built-in maximum size for PAL or NTSC when capturing video. You do
need mega amounts of RAM though, and need to use at least thousands of
colours if capturing colour images. We've used both Apple video player and
Avid Videoshop, both of which came with our machine. Maybe if the S-VHS
recorder is limiting the resolution, this would be a way to go. It
shouldn't be too hard to set up a time lapse system controlled by the
computer.

cheers,

Rosemary White
Department of Ecology and Evolutionary Biology
Monash University, Clayton, Victoria 3168, Australia
phone 61-3-9905 5670 email r.g.white-at-sci.monash.edu.au
fax 61-3-9905 5613 or rgwhite-at-vaxc.cc.monash.edu.au






From: Stephen J Murray :      smurray-at-u.Arizona.EDU
Date: Wed, 2 Oct 1996 21:15:13 -0700 (MST)
Subject: Digitalizing on the Cheap

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Hello all,

I'm a high school teacher whose been given a vintage 1980 Cambridge
StereoScan 250 SEM. It is up and running as well as expected but now I
need to figure out how to get it more flexible. The recording device is a
Polariod affair that is much too expensive for numerous high schoolers.
What was intimated was hooking up a digitalizer to the TV mode of the
viewing screen and bypass the HRRT.(?) It was also told to me that if we
wanted to used the HRRT to capture the image digitally that it would take
a bit more money as well as smarts. (Two things I'm rather short on right
now).

My mission (of which I am pleading for help with) is to find a product and
a vendor of such a digitalizer, a price, and all the pertinent information
to present at a meeting next week.

Help please!

Stephen Murray





From: DVCCO-at-aol.com
Date: Thu, 3 Oct 1996 01:58:48 -0400
Subject: Re: Universal Microscope (Aus...

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Carl Zeiss of old West Germany had merged with Jena which was the old Carl
Zeiss in East Germany before WW2.
Zeiss should have a web page in any of the browsers such as Yahoo or try
www.zeiss.com and see what happens.

Regards,

Rich
DVC Company




From: Stephen Griffiths :      s.griffiths-at-ucl.ac.uk
Date: Thu, 03 Oct 1996 07:55:57 +0100
Subject: Re: Molds

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Message-Id: {1.5.4.16.19961003065557.237f1b58-at-pop-server.bcc.ac.uk}
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Nuria Cortadellas wrote:-

} Does anyone knows some Beem flat embedding molds from polyethylene deeper
} than 3mm? we have some problems with embedding media such as nanoplast,
} lowicryls or LR-white...because the bloc is too thin and sometimes it
} breaks.
} Thanks in advanced
} Nuria Cortadellas


TAAB in the UK make an embedding mold that is similar to a standard type
capsule except that it has a full flat end. They are 8mm in diameter. They
have a snap lid which is helpful if you are embedding resins like Lowicryl
and LR White where you may wish to exclude air. Orientation of specimens in
the end of the capsule can be difficult but is usually possible with a bit
of ingenuity.

I don't know if TAAB products are available in your locality from another EM
supplier.
If not then details are:-

TAAB Laboratories Equipment Ltd.
3 Minerva House, Calleva Industrial Park
Aldermaston, Berkshire, England. RG7 8NA
Phone 01734 817775 (local UK number)
Fax 01734 817881 (local UK Number)

Cat No. C094 TAAB Capsule 8mm flat, polythene
Cat N0. C095 TAAB Capsule 8mm flat, polypropylene

Regards
Stephen Griffiths


{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
Stephen Griffiths e-mail: s.griffiths-at-ucl.ac.uk
Visual Science Department
Institute of Ophthalmology Tel: 0171 608 6914
London EC1V 9EL UK Fax: 0171 608 6850
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}





From: Keith Ryan :      KPR-at-WPO.NERC.AC.UK
Date: Thu, 03 Oct 1996 08:41:24 +0000
Subject: Universal Microscope (Aus Jenna). Optical-microscope information.

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Message-Id: {s2537b8a.058-at-WPO.NERC.AC.UK}
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Alternatives -Reply

Dear Carlos

I was in Jena (in former East Germany) about two years ago.
The entire Jena Zeiss complex was demolished for
redevelopment as (I beliece) a shopping complex. Only the
original planetarium was remaining, at a separate location
in the city.

The Jena company reunited with the Oberkochen company
a little while ago. There is a web site at:

http://www.zeiss.de

This also gives phone and fax for Carl Jeiss Jena GmbH:
tel. ++49 (0) 3641 64 0
fax. 0049 (0) 3641 2856 but maybe this is the planetarium?

For the Oberkochen site:
tel. 0049 (0) 7634 0 (this seems a little short?)
fax. 0049 (0)7634 20 6808

Good luck


With best wishes - Keith Ryan








From: Dr. Molnar Peter :      molnarp-at-lib.dote.hu
Date: Thu, 3 Oct 1996 11:47:31 +100
Subject: SEM atlas

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Dear Fellow Microscopists:
I would highly appreciate titles (and ISBN, address for ordering,
possibly price..) of books and/or atlases on normal and pathological
SEM material.
I'd like to use them for teaching (first myself) and diagnostic
purposes. Any suggestions would be welcome.
Thanks in advance
Peter P. Molnar
molnarp-at-lib.dote.hu




From: Michael J. Lyon :      lyonm-at-vax.cs.hscsyr.edu
Date: Thu, 03 Oct 1996 08:06:41 -0400
Subject: Staining compatibility

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Thanks for the responses. But it is apparent that I was not clear in
stating my problem. So I will give it another try.

I am trying to dissect out some very small {50 um blood vessels. The
main problem is that I cannot see them once the blood has washed out. I
would like to stain the vessels with something that is compatible with
immunohistochemical procedures using confocal microscopy. I am
interested in immunostaining for the innervation and then determining,
among other things, length density. Since most of the nerve are very
small I would like to image them using the confocal microscopy.
However, I am concerned that the vessel staining will interfere with the
fluorescence when viewed on the confocal either by scattering or
autofluorescence. Since alkaline phosphatase is present in most vessels
and is still active after paraformaldehyde fixation, this should would
work for the vessel stain. I am using rats and most likely the method
of Mayahara et al., Histochemie 11:88;1967. I hope that this has
clarified my problem and once again, any advice would be appreciated.

Thanks




From: Katherine.S.Connolly-at-Dartmouth.EDU (Katherine S. Connolly)
Date: 03 Oct 96 09:12:25 EDT
Subject: staining compatibility

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Message-id: {33132170-at-dancer.Dartmouth.EDU}

If you are fixing by perfusion,2% Evans blue in the fixative stains the vessels
without any conflict with horseradish peroxidase or for that matter the
fixation. I cannot comment on its fluorescence.
Kate Connolly




From: Gerd Schreiter :      schreiter-at-zoologie.uni-halle.de
Date: Thu, 3 Oct 1996 16:28:42
Subject: Re: Universal Microscope (Aus Jenna)

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Message-Id: {m0v8mIo-0004A8C-at-fast.net}

} http://www.zeiss.de

official address: Carl Zeiss Jena GmbH
Tatzendpromenade 1a
07745 Jena
Germany

Phone number of our representativ, if this is any help:

03641 643248 (Germany)
and Fax similar
03641 643439


regards, Gerd

* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
* *
* Dr. Gerd Schreiter University of Halle *
* Dept. Dev. Biol. Institute of Zoology *
* Domplatz 4 *
* Tel. +49 345 5526433 Halle/Saale 06099 *
* Fax +49 345 5527152 Germany *
* *
* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *




From: rigbyj4-at-cs.uleth.ca (Joel Rigby)
Date: Thu, 3 Oct 1996 10:09:12 -0600
Subject: Re: Universal Microscope (Aus Jenna)

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Subscribe




From: Peter D. Barnett :      pbarnett-at-crl.com
Date: Thu, 3 Oct 1996 09:32:02 -0700
Subject: Leeuwenhoek microscope

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Does anyone know where I can find an image of a Leeuwenhoek microscope that
I can download for use in a meeting annoucnement I am preparing?

Thanks.

Pete Barnett






From: Dave King (607)757-1248 T37/257-3 Ext deking-at-vnet.ibm.com
Date: 3 Oct 1996 09:01:08 EDT
Subject: Digitalizing on the Cheap

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Message-Id: {199610031254.HAA25380-at-Sparc5.Microscopy.Com}

To: Stephen J Murray {smurray-at-u.Arizona.EDU}
Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}
*** Reply to note of 10/03/96 00:25

Stephen,

We have an S250 Mk II, also. I've got the image store unit on
ours. It has a standard TV output (NTST). If you ask the people at
Leo, they might be able to find you an old used one, for a
decent price, considering how old it is. BTW, it's the HRRU,
"high resolution record unit."

We have a thermal printer attached to the NTST output, but the
image quality is poor. The HRRU has a resolution of 2500 verticle
lines, and type 52 Polaroid film is about 1500 lines. The NTST
should be about 350 lines. Thermal prints can be very
inexpensive, but the resolution will never be very good.

At one time we had a data acquisition board attached, and took
images from the visual display unit (don't remember line counts).
The problem was software for handling the image capture and
processing. A rudimentary version was developed locally, but then
the support was dropped.

There are many commercial people in this business now. PC based
image capture should be the best. I'm sure one can help you, but
it will be costly. I'd like to know what the best solution is,
since I'm not happy with ours, but our budgets are now very
tight, too. Digitized images can be electronically distributed and
processed as well. These are the reasons I have renewed interest.

Please keep this discussion public. I'm sure there are many
people interested in this.

. {
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: jkdye-at-ucdavis.edu (J. K. Dye)
Date: Thu, 3 Oct 1996 11:09:59 -0700 (PDT)
Subject: Help

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Help






From: tania-at-dynamotive.com (Tania Jones)
Date: Thu, 03 Oct 1996 11:15:46 -0700
Subject: grinders/polishers

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hello,

I'm looking for used bench top grinder/ polishers that are available, cheap.

Please email any information to:

tania-at-dynamotive.com

Thanks in advance,

Tania Jones





From: TLREYN-at-ccmail.monsanto.com
Date: Thu, 3 Oct 1996 15:18:57 -0500
Subject: unsubscribe

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unsubscribe tlreyn-at-ccmail.monsanto.com




From: stoughma-at-ornl.gov (Matthew A. Stough)
Date: Thu, 3 Oct 1996 17:08:48 -0400 (EDT)
Subject: DIGEST mode?

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I believe I asked this before, if so, excuse this question:

Is there a DIGEST mode for this list?

Thanks.
Matt Stough






From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Thu, 3 Oct 1996 09:07:28 -0700 (PDT)
Subject: Re: staining compatibility

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On 3 Oct 1996, Katherine S. Connolly wrote:

} If you are fixing by perfusion,2% Evans blue in the fixative stains the vessels
} without any conflict with horseradish peroxidase or for that matter the
} fixation. I cannot comment on its fluorescence.
} Kate Connolly
}
Hello,

The Evans blue will emit very strong fluoresence in red but not green.
We use it as a nice counterstain when using FITC as our primary tag.

Bob
morphology core
dermatology
Univ. of Wash.
Seattle






From: PKaan-at-prl.pulmonary.ubc.ca
Date: Thu, 3 Oct 1996 08:21:19 +0800 PST
Subject: Re: Fixation of cells for immunogold

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Hello All,

I would appreciate some pointers from anyone who is familiar with
fixation of cultured cells to be subsequently used immunogold
labeling. I have tried using 4% paraformaldehyde, 0.1%
glutaraldehyde in PBS -at- pH7.4 but the cell morphology was quite
chewed up. Thanks for your help.

Philomena Kaan
UBC Pulmonary Research Laboratory
St. Paul's Hospital
Vancouver, B.C.





From: Patty Jansma :      plj-at-manduca.neurobio.arizona.edu
Date: Thu, 03 Oct 1996 16:17:15 -0600 (MDT)
Subject: antibody production

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{Pine.SUN.3.91.961003161428.6190A-100000-at-manduca.neurobio.arizona.edu}
MIME-version: 1.0
Content-type: TEXT/PLAIN; charset=US-ASCII
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We are interested in making polyclonal antibodies to a 11 Kd purified
protein. Do anyone have a recommendation of a company to use or to stay
away from?

Patty Jansma Tel:602-621-6671
plj-at-manduca.neurobio.arizona.edu
Arizona Research Labs Division of Neurobiology
University of Arizona





From: Ron Norris :      NORRISR-at-algonquinc.on.ca
Date: Thu, 3 Oct 1996 17:21:56 EST
Subject: NanoLab 7 SEM

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Regarding your e-mail message of September 28/96 and your interest in
the NanoLab 7 SEM:

Servicing, parts, spares and technical details are available from
Semoptics Ltd. (613) 727-1698 (Voice or Fax). We are staffed by
former employees who designed and manufactured the SEM at Semco
Instruments in Ottawa, Canada from 1977-1984. The resolution of the
NL7 with Tungsten emitter was {= 70 Angstroms.

Much more information by contacting Semoptics at the above phone
number. Look forward to talking with you.

- Al Bingham




From: Sara Miller :      saram-at-acpub.duke.edu
Date: Thu, 3 Oct 1996 20:46:54 -0400 (EDT)
Subject: Re: SEM atlas

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On Thu, 3 Oct 1996, Dr. Molnar Peter wrote:

} Date: Thu, 3 Oct 1996 11:47:31 +100
} From: Dr. Molnar Peter {molnarp-at-lib.dote.hu}
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: SEM atlas
}
} Dear Fellow Microscopists:
} I would highly appreciate titles (and ISBN, address for ordering,
} possibly price..) of books and/or atlases on normal and pathological
} SEM material.
} I'd like to use them for teaching (first myself) and diagnostic
} purposes. Any suggestions would be welcome.
} Thanks in advance
} Peter P. Molnar
} molnarp-at-lib.dote.hu
}
Tissues and Organs: A Text-Atlas of Scanning Electron Microscopy
1979, R. G. Kessel and R H. Kardon
W. H. Freeman and Co., San Francisco

ISBN 0-7167-0091-3
0-7167-0090-5 pbk


Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Fri, 4 Oct 1996 12:39:00 +1000 (EST)
Subject: Re: SEM atlas

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Alternate-Recipient: allowed
Auto-Forwarded: prohibited
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Conversion: allowed
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Priority: normal
Sensitivity: Company-Confidential

Good books with SEM pictures

Scanning Nature
D. Claugher
British Museum
ISBN 0521 25705 0 Hardback
GBPound 5.5

Tissues and Organs
R.G. Kessel & R.H. Kardon
W.H. Freeman S.F.
ISBN 0-7167-0091-3
}
}





From: Probing & Structure :      p&s-at-ultra.net.au
Date: Fri, 04 Oct 1996 11:48:53 +1000
Subject: Re: again -SEM-film speed & aperture

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At 07:51 2/10/96 -0400, you wrote:
}
}
} On Tue, 1 Oct 1996, Probing & Structure wrote:
}
} } At 20:00 30/09/96 -0400, you wrote:
} } } I have recently been using a Hitachi S-570 SEM. I wanted to use Polaroid 53,
} } } but this being an older machine it does not have a setting for 800 speed
} } } film. I was considering using the recommended setting for 400 ISO film and
} } } close the lens aperture down one stop. Does anyone know if this is feasible
} } } or can they recommend a better method.
} } }
} } } Thank you
} } } Tom Bryner
} } ******************************
} } Hi Tom and whoever:
} } I believe that you cannot do better than to close down the camera
} } by one stop. The only other consideration is lens resolution. For that size
} } format best resolution for most lenses would be between f5.6 to 11. Lenses
} } designed for 35mm format are likely to have best resolution with the
} } aperture one stop more open.
} } If the image is properly in focous, the additional depths of field
} } is no consideration when photgraphing a flat screen.
} } Cheers
} } Jim Darley
} } Probing & Structure
} } } (Microscopy Supplies & Accessories)
} } PO Box 111, Thuringowa QLD 4817 Australia
} } }
} } } Phone +61 77 740 370 Fax: +61 77 892 313
} } } A great microscopy site http://www.ultra.net.au/~pns/
} }
} Years ago I did a study on the appropriate F stop to set the camera on the
} image recording unit for the SEM (JEOL-35). That instrument had a F4.5
} lens and the ability to adjust the contrast and brightness independently.
}
} The end result was interesting. The quality parameter measured was the
} number of line pairs resolved on the photographic image (Polaroid type 55
} was used since it has the resolution to see 2000 line pairs over the 4X5"
} image. The best result was F8. At smaller F stopsl (F11 and lower) the
} brigtness had to be increased which had the result of blooming- the
} phosphor was being pushed too hard. At larger F stops there was a lack of
} depth of field field resulting in the outer corners of the image being out
} of focus. Also noticed was defocusing of the image due to "mottling" of
} the negative material.
}
} Incidentally, our MRS-3 standard has patterns to help check CRT
} resolution.
}
}
} Hope this little tutorial helps.
}
} Joe Geller
} Geller Microanalytical Lab
} Topsfield, MA 01983
} www.gellermicro.com
*************************************************

Joe Geller's observations, no doubt were right. But he had to stop down the
large format lens to F8 to overcome spherical aberrations. Defocussed edges
indicate that and not a lack of depth of focous. Joe (and many SEM'ists)
have a crummy lens on their SEM.
The better solution is a good lens; but SEM manufacturers save money with
cheap lenses and would argue that the resolution is good enough, considering
the film format and SEM screen resolution.
The result is that users pay for roll film and obtain an image quality which
is no better than is attainable with 35mm format and a good macro lens.
50 ASA 35mm film easily resolves all SEM screen details. The beauty
of such 35mm system is that the camera can be motorised and integrated with
the SEM. This totally avoids double and unexposed negatives. I've done and
it works well.
Jim Darley





From: geoffa-at-amsg.austmus.gov.au (GeoffA)
Date: Fri, 4 Oct 1996 10:22:43 +1100
Subject: Re: Digitalizing on the Cheap

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Mime-Version: 1.0

Hi Stephen,

Congrats on getting an SEM into a school environment!

I kept this email that appeared on the listserver a few months ago for just
such an occasion. I hope it's helpful. I left the author's details at the
bottom for you to direct further questions. I'd suggest that if you go
this way (i.e. on a Mac) that you get hold of the public domain software
'Image' from NIH - it's a very handy Image Processing/Image Analysis
package.

Another option might be the frame grabber we have on our Cambridge S120
called 'Image Slave' which slots into a PC, and I'd get hold of Image Tools,
another pub.dom. IA/IP program for PC's. To find an 'Image Slave'
distributor in the US you might ask Steve Wisbey of OED Pty Ltd here in Oz
(sbwisbey-at-ozemail.com.au).

I realise that money it a key issue but I'd strongly recommend trying to
get a Back-Scatterd Electron detector at some stage. Kids love looking at
hairy biological things which are buggers for charging under the beam which
produces crap Secondary Electron images. The image from a BSE detector
will not be troubled by charging 99% of the time.

Good Luck,

Geoff

::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
Geoff Avern
Manager
Microscopy Laboratories
Australian Museum Email: geoffa-at-amsg.austmus.oz.au
6 College St Ph: (61)(2) 9320 6198
Sydney, Australia. 2000 Fax: (61)(2) 9320 6059
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::

------------------------------------------------------------------------
From Hasso Weiland:

} From the feedback I obtained to my previous posting it seems that there is
astrong interest for home made systems for digital imaging on analog SEMs.
Here is my cooking recipe for digital imaging on a JEOL840 using a MacIIci.
The cost of the whole system was about US$ 700 and two days of work.

Ingredients:

1 MAC ADIOS IIJr from GW Instruments, 35 Medford St. Sommerville, MA 02143,
Tel: (617) 625-4096

1 MAC ADIO ABO (Analog Breakout box)

1 9ft cable with a 14 pin connector on the microscope side, loose ends to
connect to the terminal on the ADIOS board. The cable connects on the
microscope side to connector JA2 on the rear panel of the JEOL840. This
connection will control the SEM. A BNC cable connects to the JEOL connector
NA8. This cable will carry the video signal to one of the AD channels of
the MACADIOS board. The video signal on NA8 carries the same signal which
will be displayed on the SEM screen, thus whatever signal is selected for
the SEM display, BSE, SE, or ??, will be recorded by the digital imaging
sytem.

1 small software, controlling the beam. It will deflect the beam and read
in the Video signal at each position. The resolution can be set by the
user.

-----------------------------------------------------------------

Pin connections on the JA2 connector are as following:

Pin 1: X-deflection connected to DA out (CHN0) Pin 2: Y-deflection
connected to DA out (CHN1)
Pin 4: Blank (blanks the SEM screen) connected to one of the Digital Out
channels
Pin 5: Relay (controls the relay in the JEOL840 for external scan coil
control, (this relay is build in each microscope), connected to one of the
Digital Out channels
Pin 7, 14: Ground connected to Common


The board should be set up to 10V on the DA channels. 0 Volt is in the
screen center. For the X-deflection, +9 Volts is on the left of the screen
(when sitting in front of the microscope), -9 Volt on the right side. For
the Y-deflection, -9 Volt is the top of the screen, and +9 Volt is the
bottom.

For external control, set the Digital Out which is connected to Pin 5 to
high, low will give the control back to the JEOL system.
Blank (connected to Pin 4) is on when set to high, setting this Digital Out
to low will leave the beam on (be careful, this can easily burn a black
mark on your screen, always watch your screen when setting up the system,
best is to turn brightness and contrast down).


The small C-code we are using is part of an other application (BKD
analysis), thus it is not stand alone and not necessarily helpful to
everyone. If interested, I can e-mail the code later to those who would
like to have it as a guide for their own development.

If anybody needs more help, please let me know.


Hasso Weiland
Alcoa Technical Center
Alcoa Center, PA 15069, USA
412 337-3133
weiland_h-at-atc.alcoa.com







From: Paul Vanderlinden :      orion-at-infoboard.be
Date: Fri, 4 Oct 1996 09:04:51 +0200 (MET DST)
Subject: Re: Digitalizing on the Cheap

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Dave,

Our company E.L.I. sprl (Belgium) has developped a vey powerful system for
electronic microscope grabbing: ORION 4.1.

We are sure you will understand how easy it can be to upgrade your SEM by
acquiring digital pictures with such a convivial package.

For your information, the PC minimum requirements to run ORION 4.1 are the
following:
* Pentium 100 Mhz - 16 Mb RAM
* ISA slot 16 bits full length
* VGA graphic card 1 Mb

The price for the ORION 4.1 package (hardware card + software) is 8.000-US$.

The "installation" includes:
* the physical installation itself and the tests
* a full training for the users
* one year hot line guarantee (in case of on site service, the travelling
costs will be charged)

The price for this service depends on the country (do we have a distributor
or not) and/or the technical capability of the customer to install itself
the connection with on line help (phone, fax or Email) from our technical
service.

Don't hesitate to contact me for more information and/or have a look to our
http site:
http://www.microscopy-uk.org.uk




At 09:01 03/10/1996 EDT, you wrote:

}
} There are many commercial people in this business now. PC based
} image capture should be the best. I'm sure one can help you, but
} it will be costly. I'd like to know what the best solution is,
} since I'm not happy with ours, but our budgets are now very
} tight, too. Digitized images can be electronically distributed and
} processed as well. These are the reasons I have renewed interest.
}
} Please keep this discussion public. I'm sure there are many
} people interested in this.
}
} . {
} } { {
} } } ===========} Dave King { { {
} } } } ------------------------------------------------------ { { { {
}
}


Best regards,



Paul Vanderlinden.
Sales Manager.

=========================================================================
To contact us:

E.L.I. sprl

Technical support:
Jean-Louis Leclef: Phone: +32 67 21 25 07
Fax : +32 67 22 09 53
Email: jleclef-at-hypercon.com
Sales support:
Paul Vanderlinden: Phone: +32 2 215 20 02
Fax : +32 2 726 08 65
Email: orion-at-infoboard.be
=========================================================================







From: Margareta Halin :      Margareta.Halin-at-ah.slu.se
Date: Fri, 4 Oct 1996 09:06:49 +0100
Subject: re: Fixation of cells for immuno-gold staining

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Hi!

I suggest that you increase the concentration of glutaraldehyde to 1%. (4%
PFA + 1% GA).
When I fix cultured cells, I heat the fixative to 37 degr. C, and fix the
cells taken directly out of the chamber -after one wash with buffer of the
same temperature -(if they're not free-floating). I let them cool down in RT
for a while, and then put them in the fridge for a couple of hours / over
night. On the other hand; when you embed the cells in plastic that is
suitable for immuno-gold staining + omit the osmium treatment, you don't
really see what you're used to...

Good luck!

Margareta.Halin-at-ah.slu.se
Dept. of Anatomy and Histology
Sveriges LantbruksUniversitet





From: Robert Fisher :      rmfisher-at-u.washington.edu
Date: Fri, 4 Oct 1996 04:03:39 -0700 (PDT)
Subject: TEM Microscopist Position

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A post-doc or visiting scientist position is available at the
University of Washington in Materials Science & Engineering
for studies of metal - oxide interfaces using TEM and ELS.
Other duties include help with supervision of TEM operation
and general EM maintenance.

For further information please send a brief resume by email to -

Dr. R. M. Fisher
Director - Electron Microscopy Consortium
Univ. of Washington, Seattle, WA

rmfisher-at-u.washington.edu







From: kris-at-elod.vein.hu (Kris Kovacs)
Date: Fri, 4 Oct 1996 12:06:44 +0100
Subject: Leeuwenhoek microscope zipped tif

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--=====================_844459451==_
Content-Type: text/plain; charset="us-ascii"

Dear Pete and all Microscopists interested:

Attached you will find a sketch of the Leeuwenhoek microscope scanned as a
high resolution tiff image (slightly less than 500 kb when uncompressed).
Legend to the figure:

a: Base
b: Lens
c: Height adjustment (coarse)
d: Specimen holder and height adjustment (fine)
e: Focusing screw

Enjoy it!

T.G.I.F.

Kris

--=====================_844459451==_
Content-Type: application/mac-binhex40; name="LEEUWTIF.ZIP"
Content-Disposition: attachment; filename="LEEUWTIF.ZIP"


(This file must be converted with BinHex 4.0)

--=====================_844459451==_
Content-Type: text/plain; charset="us-ascii"



*************************************************************************
Kristof KOVACS
Associate Professor
University of Veszprem, Central Laboratory
P.O.Box 158, Veszprem, HUNGARY
H-8201
Phone: +36-(88)-421-684
*************************************************************************

--=====================_844459451==_--





From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Fri, 4 Oct 1996 08:05:07 -0500
Subject: DONOT SEND IMAGES to the LISTSERVER

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Message-Id: {v03007800ae7abadb740d-at-[206.69.208.21]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Colleagues...

We went over this long ago. NEVER, NEVER, post images
compressed or otherwise to the listserver. If you wish
to send an image to an individual that has requested it
that is fine. But do not fill the worlds mailboxes with
images, epecially using this forum. Too many people will
have their mailboxes filled and it will result in bouncing
Email messages (to me!) saying that there is a problem with
user XYZ receiving Email.

If needed, I can and will supply FREE space on the MSA
Anonymous FTP Site as well and my ANL Site in the Image
Library sections for miscellaneous non-copyrighted and
free/public domain images.


Nestor
Your Friendly Neighborhood SysOp






From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Fri, 4 Oct 1996 08:05:07 -0500
Subject: DONOT SEND IMAGES to the LISTSERVER

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {v03007800ae7abadb740d-at-[206.69.208.21]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Colleagues...

We went over this long ago. NEVER, NEVER, post images
compressed or otherwise to the listserver. If you wish
to send an image to an individual that has requested it
that is fine. But do not fill the worlds mailboxes with
images, epecially using this forum. Too many people will
have their mailboxes filled and it will result in bouncing
Email messages (to me!) saying that there is a problem with
user XYZ receiving Email.

If needed, I can and will supply FREE space on the MSA
Anonymous FTP Site as well and my ANL Site in the Image
Library sections for miscellaneous non-copyrighted and
free/public domain images.


Nestor
Your Friendly Neighborhood SysOp






From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Fri, 04 Oct 1996 08:44:52 -0400
Subject: Re: DIGEST mode?

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Message-Id: {1.5.4.32.19961004124452.006a5154-at-biotech.ufl.edu}
X-Sender: sdw-at-biotech.ufl.edu
X-Mailer: Windows Eudora Light Version 1.5.4 (32)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


Nestor keeps an archive of everything on the list at the MSA home
page "http://www.msa.microscopy.com/" Go to the "Microscopy and
Microanalysis Software Library" Click on the "pub" directory and then on the
"MicroscopyListserverArchives" directory. In ther you will find everything
from about 1994 to current. It is not convienient nor searchable, but it is
all there.
Another option is "Tips & Tricks" at the web address listed at the
end of this message. I maintain a biologic archive of the info posted to
this list since about late 1994. It is more convient to use but limited to
what I wanted to put in. If there is something you are specifically looking
for or something you remember seeing recently but can't find, let me know.
So far the archive is rather labor intensive and is usually about a month
behind. I hope this helps.



At 05:08 PM 10/3/96 -0400, you wrote:
} I believe I asked this before, if so, excuse this question:
}
} Is there a DIGEST mode for this list?
}
} Thanks.
} Matt Stough
}
}
}
}




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
GO GATORS
Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 352-392-1295
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "





From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Fri, 4 Oct 1996 09:30:42 -0500
Subject: Re: Fixation of cells for immunogold

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For cultured human colon adenocarcinoma cells, I use
2% freshly depolymerized paraformaldehyde (NOT formalin!)
70 mM NaCl
30 mM HEPES
5 mM CaCl2
pH 7.4

Cells like divalents (Ca2+) around during fixation.
The osmolarity needs to account for the initial contribution of the
aldehydes (a tricky situation since they are somewhat permeable but this
increases with time.
The infiltration into plastic should be gradual (2:1 and 1:1 solvent:resin).



} Hello All,
}
} I would appreciate some pointers from anyone who is familiar with
} fixation of cultured cells to be subsequently used immunogold
} labeling. I have tried using 4% paraformaldehyde, 0.1%
} glutaraldehyde in PBS -at- pH7.4 but the cell morphology was quite
} chewed up. Thanks for your help.
}
} Philomena Kaan
} UBC Pulmonary Research Laboratory
} St. Paul's Hospital
} Vancouver, B.C.

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(573)-882-4712 (voice)
(573)-882-0123 (fax)






From: Doug Cromey :      dcromey-at-ccit.arizona.edu
Date: Fri, 04 Oct 1996 08:48:58 -0700
Subject: New WWW Page announcement

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {1.5.4.32.19961004154858.006890bc-at-ccit.arizona.edu}
X-Sender: dcromey-at-ccit.arizona.edu
X-Mailer: Windows Eudora Light Version 1.5.4 (32)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear Confocal & Microscopy Listserv subscribers,

I'd like to make you aware of a suite of WWW pages that I've put together
that touch on many of the topics related to biological microscopy and
imaging. My target audience is students and staff (since that's who I work
with the most), although I suspect "old hands" will find some useful links
as well.

The main page is entitled "Microscopy and Imaging on the WWW". Related
pages touch on Histology, Electron Microscopy, Confocal Microscopy, Digital
Imaging and a page on free magazine subscriptions that might be of interest
to microscopists.

The URL is (I had no control over how long this is):
http://www.pharm.Arizona.edu/centers/tox_center/swehsc/exp_path/m-i_onw3.html

Your comments & feedback on this work in progress would be appreciated.

Yours,

Doug Cromey
.....................................................................
: Douglas W. Cromey, M.S. Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212A) P.O. Box 245044 :
: (voice: 520-626-2824) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: dcromey-at-ccit.arizona.edu) :
:...................................................................:
http://www.pharm.arizona.edu/exp_path.html





From: Sara Miller :      saram-at-acpub.duke.edu
Date: Fri, 4 Oct 1996 10:45:41 -0400 (EDT)
Subject: re: Fixation of cells for immuno-gold staining

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On Fri, 4 Oct 1996, Margareta Halin wrote:

} Date: Fri, 4 Oct 1996 09:06:49 +0100
} From: Margareta Halin {Margareta.Halin-at-ah.slu.se}
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: re: Fixation of cells for immuno-gold staining
}
} Hi!
}
} I suggest that you increase the concentration of glutaraldehyde to 1%. (4%
} PFA + 1% GA).
} When I fix cultured cells, I heat the fixative to 37 degr. C, and fix the
} cells taken directly out of the chamber -after one wash with buffer of the
} same temperature -(if they're not free-floating). I let them cool down in RT
} for a while, and then put them in the fridge for a couple of hours / over
} night. On the other hand; when you embed the cells in plastic that is
} suitable for immuno-gold staining + omit the osmium treatment, you don't
} really see what you're used to...
}
} Good luck!
}
} Margareta.Halin-at-ah.slu.se
} Dept. of Anatomy and Histology
} Sveriges LantbruksUniversitet
}


However, be aware that glutaraldehyde can reduce or destroy some
antigenic sites. The general rule is that the higher the glutaraldehyde
concentration, the better the ultrastructure, but the worse the
immunostaining. The only way to know if your particular antigen remains
active after glut is to try it. Usual percents used range from about 0.1
to 1%, mixed with paraformaldehyde (4-8 %). Also, sometimes staining
decreases with increased storage of specimens in aldehydes, even
formaldehyde. If you're starting with an unknown, your best bet is to
keep the glut low (we don't use it), and the fixation time short (1-2
hrs). Then you can test to see if more glut, longer fixation times, or even
osmium fixation (not usually used except in freeze substitution), can be
tolerated. Margareta's statement that the ultrastructure of
non-osmicated tissue is not what you're used to seeing is very true.
Maybe your cells aren't chewed up--just not stained conventionally.

Sara


Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Fri, 4 Oct 1996 11:30:03 -0700 (PDT)
Subject: Re: Fixation of cells for immunogold

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On Thu, 3 Oct 1996 PKaan-at-prl.pulmonary.ubc.ca wrote:

} Hello All,
}
} I would appreciate some pointers from anyone who is familiar with
} fixation of cultured cells to be subsequently used immunogold
} labeling. I have tried using 4% paraformaldehyde, 0.1%
} glutaraldehyde in PBS -at- pH7.4 but the cell morphology was quite
} chewed up. Thanks for your help.
}
} Philomena Kaan
} UBC Pulmonary Research Laboratory
} St. Paul's Hospital
} Vancouver, B.C.
}
}
Hello,

If the cells are to be post embed labelled I would worry about too strong
of fixation in fear of loosing the immunolabelling capacity.

You might try a 2-4% para in sorensons (so there is no NaCl in the
formula) or a combination of para and picric acid in sorensons buffer such
as a Zamboni fixative, if you are post embed labelling on sections.

We have noticed a problem when there is NaCl present in the fix, with poor
morphology.

Bob
Morphology Core
Derm
U of Wash
Seattle





From: GREG VAUGHN MARTIN :      gmartin-at-welchlink.welch.jhu.edu
Date: Fri, 4 Oct 1996 16:47:04 -0400 (EDT)
Subject: Re: Fixation of cells for immunogold

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On Thu, 3 Oct 1996 PKaan-at-prl.pulmonary.ubc.ca wrote:

} Hello All,
}
} I would appreciate some pointers from anyone who is familiar with
} fixation of cultured cells to be subsequently used immunogold
} labeling. I have tried using 4% paraformaldehyde, 0.1%
} glutaraldehyde in PBS -at- pH7.4 but the cell morphology was quite
} chewed up. Thanks for your help.
}
} Philomena Kaan
} UBC Pulmonary Research Laboratory
} St. Paul's Hospital
} Vancouver, B.C.
}
}

You might try using a different buffer -- I've never had very good
results fixing in PBS. We generally use 3% freshly (that's important)
depolymerized formaldehyde and 0.05% glut. in a modified Hanks buffer.
The more glut. your antigens will tolerate, the better. To get the
membrane contrast closer to what you are used to seeing by
conventional EM,try en bloc staining with Uranyl Acetate (which can help
to improve the retention of antigenicity) and Tannic Acid (which can
reduce antigenicity, so try some tissue +TA and some -TA). The
composition of the modified Hanks fixative and protocols for en bloc
staining can be found in:

J. Histochem. Cytochem. 40:845-857 '92.

There is (are?) a myriad of protocols for immunoEM. I sort of
inherited this one and found it to work very well for many cell types and
antigens, using either LRGold or Lowicryl K4M resins.

Happy Labeling!

Greg Martin
gmartin-at-welchlink.welch.jhu.edu
Dept. Cell Biology and Anatomy
Johns Hopkins School of Medicine





From: John G. Aghajanian, Ph.D. :      JOHNA-at-SCI.WFBR.EDU
Date: Fri, 04 Oct 1996 14:41:12 -0700
Subject: Re: Fixation of cells for immuno-gold staining

Contents Retrieved from Microscopy Listserver Archives
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Sara Miller wrote:
}
} On Fri, 4 Oct 1996, Margareta Halin wrote:
}
} } Date: Fri, 4 Oct 1996 09:06:49 +0100
} } From: Margareta Halin {Margareta.Halin-at-ah.slu.se}
} } To: microscopy-at-Sparc5.Microscopy.Com
} } Subject: re: Fixation of cells for immuno-gold staining
} }
} } Hi!
} }
} } I suggest that you increase the concentration of glutaraldehyde to 1%. (4%
} } PFA + 1% GA).
} } When I fix cultured cells, I heat the fixative to 37 degr. C, and fix the
} } cells taken directly out of the chamber -after one wash with buffer of the
} } same temperature -(if they're not free-floating). I let them cool down in RT
} } for a while, and then put them in the fridge for a couple of hours / over
} } night. On the other hand; when you embed the cells in plastic that is
} } suitable for immuno-gold staining + omit the osmium treatment, you don't
} } really see what you're used to...
} }
} } Good luck!
} }
} } Margareta.Halin-at-ah.slu.se
} } Dept. of Anatomy and Histology
} } Sveriges LantbruksUniversitet
} }
}
} However, be aware that glutaraldehyde can reduce or destroy some
} antigenic sites. The general rule is that the higher the glutaraldehyde
} concentration, the better the ultrastructure, but the worse the
} immunostaining. The only way to know if your particular antigen remains
} active after glut is to try it. Usual percents used range from about 0.1
} to 1%, mixed with paraformaldehyde (4-8 %). Also, sometimes staining
} decreases with increased storage of specimens in aldehydes, even
} formaldehyde. If you're starting with an unknown, your best bet is to
} keep the glut low (we don't use it), and the fixation time short (1-2
} hrs). Then you can test to see if more glut, longer fixation times, or even
} osmium fixation (not usually used except in freeze substitution), can be
} tolerated. Margareta's statement that the ultrastructure of
} non-osmicated tissue is not what you're used to seeing is very true.
} Maybe your cells aren't chewed up--just not stained conventionally.
}
} Sara
}
} Sara E. Miller, Ph. D.
} P. O. Box 3020
} Duke University Medical Center
} Durham, NC 27710
} Ph: 919 684-3452
} FAX: 919 684-8735


Hello folks,

Here's a tip that will help your unosmicated/immunostained sections look
more aesthetically pleasing. After you have immunostained your
sections, presumably they are on nickel or gold grids, run the grids
through a series of droplets which parallel a routine fixation: hard fix
in 1-2% GA, buffer washes, OsO4, washes, UA, wash, blot dry and scope.
You need only incubate for 10-15 min. in the fixes and 5 min. in the
washes and so on. I've done this with LR White and Lowicryl and it
works well. My colleagues see fine structure which more closely
resembles "routinely" fixed TEM specimens and have their imunno-gold
staining as well. Kind of like having your pie and eating it too. Try
it - you'll like it!

Cheers,

John Aghajanian
Worcester Foundation for Biomedical Research




From: kris-at-elod.vein.hu (Kris Kovacs)
Date: Fri, 4 Oct 1996 19:13:35 +0100
Subject: Apologies for sending in an image

Contents Retrieved from Microscopy Listserver Archives
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Dear Colleagues,

Sorry for doing something wrong as . It was a coincidence that I saw the
posting of Pete Barnett requesting an image of Leeuwenhoek microscope and
just got a nice drawing of what is considered the very first useful
microscope. Being happy with this finding I thought this clear drawing might
be of interest to all microscopists considering the roots equally important
to the newest findings or whatever. Sorry again, I will never do this in the
future.

Kris



*************************************************************************
Kristof KOVACS
Associate Professor
University of Veszprem, Central Laboratory
P.O.Box 158, Veszprem, HUNGARY
H-8201
Phone: +36-(88)-421-684
*************************************************************************





From: mboucher-at-isd.net
Date: Fri, 4 Oct 1996 21:33:04 +0000
Subject: Help on Polarized Microscopy in Biology?

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I have been asked to give a presentation to a group of Microscope
salespersons on PLM and specifically on the geological aspects. That
is no problem, but some of their customers are ?biologists? or
non-geological and are using some compensators in their research.
They would like to know how to use them and what they are for. I can
discuss how to make the appropriate measurements, but what
materials are they measuring and what does that tell them? They are
using Berek, Brace-Kohler, Erhinghaus and senarmont compensators.
Anyone know what the ususal biological applications are?
Thanks for any advice. You can e-mail me direct. If anyone else is
curious, I can mail out a summary.
Regards,
Mike
================================================
Michael L. Boucher Sr. mboucher-at-isd.net
13345 Foliage Avenue
Apple Valley, MN 55124-5603 Ph 612-432-8836
================================================




From: Dasce-at-aol.com
Date: Fri, 4 Oct 1996 23:00:25 -0400
Subject: Subscribing to list.

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Subscribe.




From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Fri, 4 Oct 1996 20:23:54 -0800
Subject: Re: Digitalizing on the Cheap

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X-Sender: mager-at-pop.unixg.ubc.ca
Message-Id: {v01510100ae7b8e6c91c8-at-[137.82.222.214]}
Mime-Version: 1.0
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Dear Stephen,
There is a relatively inexpensive product to get an SEM image into a
computer from GW Electronics, called Printerface. Larry Glassman of GW says
it is a mail-order-type product, easy to install. Contact him at:
GW Electronics
6981 Peachtree Industrial Blvd.
Norcross GA 30092
Phone: 404-449-0707
Fax: 404-449-0284
I use a more expensive product, called PCI, and I find it is a wonderful
way to show the SEM image to a class full of students or print out laser
copies for all.
Luck
Mary.

Stephen Murray wrpte:

} I'm a high school teacher whose been given a vintage 1980 Cambridge
} StereoScan 250 SEM. It is up and running as well as expected but now I
} need to figure out how to get it more flexible. The recording device is a
} Polariod affair that is much too expensive for numerous high schoolers.
} What was intimated was hooking up a digitalizer to the TV mode of the
} viewing screen and bypass the HRRT.(?) It was also told to me that if we
} wanted to used the HRRT to capture the image digitally that it would take
} a bit more money as well as smarts. (Two things I'm rather short on right
} now).
}
} My mission (of which I am pleading for help with) is to find a product and
} a vendor of such a digitalizer, a price, and all the pertinent information
} to present at a meeting next week.
}
} Help please!
}
} Stephen Murray

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Jacob Bastacky :      SJBastacky-at-lbl.gov
Date: Sat, 05 Oct 1996 11:31:18 -0800
Subject: EM Safety Book

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Message-Id: {3256B785.212A-at-lbl.gov}

Has the second edition of the Electron Microscopy Safety Handbook been
published?




From: bozzola-at-siu.edu (John J. Bozzola)
Date: Sat, 5 Oct 1996 20:03:01 -0600
Subject: Re: EM Safety Book

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X-Sender: bozzola-at-saluki-mail.fiber2.siu.edu
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The Second Edition of The Electron Microscopy Safety Handbook edited by
Vernon C. Barber and Joseph A. Mascorro has been published for over a year.
It is available from San Francisco Press, Inc., Box 426800, San Francisco,
CA 94142-6800. I belive it is -at- $15-18. It has ISBN number of:
0-911302-72-7.

####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: EE NG :      el.ng-at-student.qut.edu.au
Date: Sun, 06 Oct 1996 12:06:51 +1000 (EST)
Subject: unsubscribe

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Please unsubscribe me from your list.





From: Michael D. Warfield :      warfield-at-current.BarePower.net
Date: Sat, 5 Oct 1996 18:10:35 -0700
Subject: unsubscribe

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subscribe warfield-at-mail.barepower.net




From: Michael D. Warfield :      warfield-at-current.BarePower.net
Date: Sun, 6 Oct 1996 08:59:28 -0700
Subject: subscribe

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From: Tom Bryner :      brynert-at-mcmaster.ca
Date: Sun, 6 Oct 1996 13:38:21 -0400 (EDT)
Subject: S-570 SEM and Polaroid summary

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Thanks for all the suggestions on my question about adjustments necessary to
the Hitachi S-570 when using an 800 speed film(Polaroid 53). The consensus
of opinion was that changing the f stop is a viable method. Also it was
suggested that calibration of the contrast and brightness would be necessary.

I changed the f stop from f16 to f22 with a scanning time of 100 seconds and
adjusted the contrast and brightness settings. To compare I reduced the scan
time to 50 seconds and used an f stop of f16. The resulting prints of these
two tests were comparable with each other. I would say that they were
slightly less sharp than using Polaroid 52 (ASA 400) at a scanning speed of
100 seconds and a f stop of f16, probably as to be expected. The results
were acceptable though.

When time and circumstance allows a thorough calibration of the video signal
will be done.

Tom Bryner





From: Dr. IGOR LAPSKER :      LAPSKER-at-barley.cteh.ac.il
Date: Mon, 7 Oct 1996 10:22:27 +200
Subject: thanks and bug

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Dear Phil Fraundorf

Many thanks for your program "EPCfocus". It is very good for teaching!
May be you know some simulation programs for SEM? It is very interesting
for me.
I cannot link to programs "ChipSi" and "VBimage". Whatis the problem?

I look forward to hearing from you in the near future.
Thanking you beforehand for you cooperation.

Whis best wishes,
Dr. Igor Lapsker
Center for Technological Education Holon
Lapsker-at-barley.cteh.ac.il




From: ayache-at-csnsm.in2p3.fr (Ayache Jeanne)
Date: Mon, 7 Oct 1996 11:57:11 +0000
Subject: telephone number change in University

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Message-Id: {199610070957.LAA04131-at-csn-hp.in2p3.fr}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} } } } } } }
} } } } } } } Dear coleague,
} } } } } } }
} } } } } } } From october the 1st the telephone numbers of Orsay University have
} } } } } } } change.
} } } } } } }
} } } } } } } PLease not my new coordinates in Orsay
} } } } } } } With the pleasure to see you in a next future
} } } } } } }
} } } } } } } Dr Jeanne Ayache,
} } } } } } } CSNSM - CNRS,
} } } } } } } batiment 108,
} } } } } } } 91405 Orsay campus,
} } } } } } } France,
} } } } } } } Tel : 01 69 15 52 19,
} } } } } } } fax : 01 69 15 52 68,
} } } } } } } email : ayache -at-csn-hp.in2p3.fr
} } } } } } }
} } } } } } }
} } } } } }






From: Dr. IGOR LAPSKER :      LAPSKER-at-barley.cteh.ac.il
Date: Mon, 7 Oct 1996 10:30:04 +200
Subject: Thahks

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Dear Phil Fraundorf

Many thanks for your program "EPCfocus". It is very good for teaching!
May be you know some simulation programs for SEM? It is very interesting
for me.
I cannot link to programs "ChipSi" and "VBimage". Whatis the problem?

I look forward to hearing from you in the near future.
Thanking you beforehand for you cooperation.

Whis best wishes,
Dr. Igor Lapsker
Center for Technological Education Holon
Lapsker-at-barley.cteh.ac.il




From: csedax-at-alpha.arcride.edu.ar
Date: Thu, 26 Sep 1996 11:11:43 -2036
Subject: thanks

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Hi all!

Thanks for answer my question refered to the tensile and torsion test by SEM,
what you send me, was an important help to complete my thesis, thanks again

Daniel F. Imbert




From: Kevin Mackenzie :      nhi691-at-abdn.ac.uk
Date: Mon, 7 Oct 1996 10:36:52 +0100 (bst)
Subject: SCOTTISH MICROSCOPY MEETING

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24th SCOTTISH MICROSCOPY GROUP SYMPOSIUM

ABERDEEN EXHIBITION and CONFERENCE CENTRE,
Wednesday 13 November 1996

Here is our finalised programme:

Biological Immunocytochemistry -Dr. Chris Hawes

A simple immunogold silver (IGSS) technique for light microscopy of =0Aresi=
n sections - Ian Roberts

The principle and benefits of energy filtering electron microscopy - Dr. =
=0ARichard Bauer

A new dry transfer system for EDX of optimally dried cryosections - Dr. =0A=
Andrew Johnson

Variable pressure SEM: principles and applications - Dr. Peter Clark

Visualising neurons using Golgi impregnation and confocal imaging of =0Aint=
racellular dyes - Dr. Debbie Brown

Image Archiving - Dr. Andy Yarwood

A novel image analysis and data visualisation method for the study of =0Abi=
ological structures - Dr. Steven Campbell

The cost for registration will be =A320.

For further information etc. please email me. =20

Kevin Mackenzie
Tillydrone E.M. Unit
University of Aberdeen
Aberdeen
Tel 01224-272847
Fax 01224-272396
email k.s.mackenzie-at-abdn.ac.uk





From: slimbach-at-facstaff.wisc.edu (Steve Limbach)
Date: Mon, 7 Oct 1996 11:09:23 -0600
Subject: messages

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Message-Id: {v02130503ae7ee91eb65e-at-[144.92.19.80]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Is it possible to exclude subscribe and unsubscribe messages from the
general messages that go out on the listserver? This would help keep down
the traffic and save all of us from having to dump sll this extraneous
mail!! Thanks, Steve

University of Wisconsin-Madison
Robert M. Bock Laboratory
1525 Linden Dr
rm#527
Madison, WI 53706

Work (608) 262-4581
Fax (608) 262-4570
Home (608) 837-9566

slimbach-at-facstaff.wisc.edu






From: Daniele Spehner :      daniele.spehner-at-etss.u-strasbg.fr
Date: Mon, 07 Oct 1996 19:09:44 +0100
Subject: photography

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Message-ID: {32594768.112D-at-etss.u-strasbg.fr}

Bonjour,

I have just finished equipping my lab with the essentials for
TEM and finally I need to get the pictures out of the scope and onto
print. Can anyone recommend a good professional enlargement system for 4
x 5 inch negatives?

Thank you, Danièle Spehner




From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Mon, 7 Oct 1996 11:25:52 -0700
Subject: update on Cu detection

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Message-Id: {199610071821.LAA05915-at-cats.ucsc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Here is an update on the Cu concentration in the plant trichomes. Latest
bulletin from trichome central is that the concentration is probably closer
to 0.1% Cu.

Now that's a horse of a different color!

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
FAX (408) 429-0146
jmkrupp-at-cats.ucsc.edu






From: Donna Wagahoff :      DWAGAHOF-at-wpsmtp.siumed.edu
Date: Mon, 07 Oct 1996 13:42:30 -0600
Subject: Flatbed Scanner Demo

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Message-Id: {s2590a30.088-at-wpsmtp.siumed.edu}
X-Mailer: Novell GroupWise 4.1

We need to digitize an entire 3.25 in. by 4.25 in. TEM negative and at the
same time, maintain resolution adequate to distinguish the morphometry
of the vesicles in synaptic terminals.
Our CCD camera is unable to give us the resolution to distinguish the
shape of the vesicles, so we were told that a flatbed scanner or a high
resolution digital camera would solve our problem. We have an
appointment for a demo of the digital camera but we also would like to
have a scanner demo. Please let me know, if you are a representative
who can demo a flatbed scanner for us or if you successfully use a
flatbed scanner for resolution requirements similar to our needs.
Thanks!

Donna Wagahoff
SIU School of Med.
P.O. Box 19230
Springfield, Il. 62794-1220

217-782-0898

fax 217-524-3227





From: Steve Limbach on Mon, Oct 7, 1996 1:49 PM
Date: 7 Oct 1996 13:46:51 -0500
Subject: messages

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Message-ID: {n1367425482.99319-at-msmail.tmc.tulane.edu}

I agreed, particularly when the sender does not include a header or subject!

Cesar D. Fermin, Ph.D. Professor of Pathology & Otolaryngology
http://www1.omi.tulane.edu/departments/pathology/fermin/cdftop.html
Fax 504 587-7389 & Voice 584-2521, Internet: fermin-at-tmc.tulane.edu

_______________________________________________________________________________

Is it possible to exclude subscribe and unsubscribe messages from the
general messages that go out on the listserver? This would help keep down
the traffic and save all of us from having to dump sll this extraneous
mail!! Thanks, Steve

University of Wisconsin-Madison
Robert M. Bock Laboratory
1525 Linden Dr
rm#527
Madison, WI 53706

Work (608) 262-4581
Fax (608) 262-4570
Home (608) 837-9566

slimbach-at-facstaff.wisc.edu







From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Mon, 7 Oct 1996 10:53:26 -0700
Subject: Microscopic detection of Cu?

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {199610071749.KAA27265-at-cats.ucsc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Greetings:

I am trying to help a user of our lab solve a problem involving locating
and quantifying small concentrations of copper in plant cells.

Here is the setup: Small plants are grown in soil high in copper. These
plants accumulate copper in the trichomes (hairs) of the plant ( we think).


Here is the question: How can it be demonstrated that copper is actually
accumulated in the trichomes and how closely can we approximate its
concentration?

I have tried helping her by doing some quick and dirty SEM/EDS. I could not
detect any copper in the trichomes (maybe I wasn't looking hard enough).
She reports that there should be around 1% copper or less per dry weight of
tissue. I suggested that that amount maybe hard to pin down using EDS, but
I told her I would check it out with the experts to see what a reasonable
minimum detectable amount might be (OK experts, what do you think?).

I also cautioned her regarding the pitfalls of EDS on biological samples,
suggesting that she may want to explore alternatives to her preparation
technique. She is currently fixing and embedding in paraffin so she can
section for the light microscope. If freezing and other cryo techniques are
the best way to go, I could use some help (and back-up) in advising her of
how far to go and the chance of success. Is simple freezing enough or does
she have to find someone with a cryo stage to keep everything in place
throughout the analysis?

Finally, do you think there is any future in this type of investigation? If
so, do you have any ideas of where we might be able to find the equipment
needed to proceed? If not, do you have any alternatives you could share?

Perhaps I am too pessimistic, maybe I don't know what I am talking about.
Either way it would help us to get some outside opinions and ideas about
this problem.

Thanks,

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
FAX (408) 429-0146
jmkrupp-at-cats.ucsc.edu






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Mon, 07 Oct 1996 16:37:42 -0500
Subject: Re: messages

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Message-Id: {199610072241.RAA11685-at-watson.bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

If they are made to the proper server rather than to the "general" server,
they won't go out over the listserver. Review the instructions that you
received when you subscribed.

Joiner

At 11:09 AM 10/7/96 -0600, you wrote:
} Is it possible to exclude subscribe and unsubscribe messages from the
} general messages that go out on the listserver? This would help keep down
} the traffic and save all of us from having to dump sll this extraneous
} mail!! Thanks, Steve
}
} University of Wisconsin-Madison
} Robert M. Bock Laboratory
} 1525 Linden Dr
} rm#527
} Madison, WI 53706
}
} Work (608) 262-4581
} Fax (608) 262-4570
} Home (608) 837-9566
}
} slimbach-at-facstaff.wisc.edu
}
}
}
}





From: keller-at-boulder.nist.gov (Bob Keller)
Date: Mon, 7 Oct 1996 15:37:05 -0700
Subject: Call for Papers-MRS Microelectronics Reliability

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X-Sender: keller-at-arc1.mrd.bldrdoc.gov
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Following Scott Walck's heads up on the TEM specimen prep. symposium, here
is a repeat of the call for papers for the Materials Reliability in
Microelectronics Symposium. Author instructions and meeting information
are available at the web site listed at the end of this message. Abstract
deadline is November 1, 1996.

Bob Keller

===================================================

CALL FOR PAPERS

Materials Research Society 1997 Spring Meeting
March 31 - April 4, 1997
San Francisco, California

ABSTRACT DEADLINE: November 1, 1996

Symposium J: Materials Reliability in Microelectronics VII

The inexorable drive for increased integrated circuit functionality and
performance places growing demands on the metal and dielectric thin films
used in fabricating these circuits, as well as spurring demand for new
materials applications and processes. In addition to meeting performance
and manufacturability requirements, these materials and processes must
yield circuits that operate reliably for many years. Achieving these goals
requires a better understanding of the relationship of thin-film material
properties and manufacturing processes to reliability degradation mechanisms.

This symposium is a continuation of the series that seeks to foster
this understanding. The aim is to provide a forum to bring together
researchers from industry and universities to discuss fundamental mechanisms
and materials properties pertinent to materials reliability issues in
the manufacture of submicron integrated circuits.

Papers are solicited in the following and related areas:

+ Reliability of metallic thin films and interconnects
- Electromigration in lines, contacts, vias
- Stress relaxation and stress voiding
- Metal microstructure: grain growth, texture, precipitate formation
- Effects of microstructure on electromigration
- Intermetallic formation and diffusion barriers
- New interconnect metallizations/novel alloys; alternative dielectrics
+ Reliability of dielectric thin films
- Physics and chemistry of gate dielectric breakdown, including
breakdown mechanisms, intrinsic vs. extrinsic failure, and failure analysis
- Reliability, yield and gate stack processing: growth conditions,
substrate effects, impurities, cleaning and plasma damage
- Relation of reliability to leakage and tunneling currents, charge
trapping and defect generation
- Special reliability considerations for ultrathin ( { 5nm) gate dielectrics
- Materials issues in hot-carrier reliability
+ Mechanical stress and strains in films, lines and device structures:
deformation, fracture, adhesion, simulation and modeling.
+ Novel analytical and measurement techniques
+ Reliability modeling and simulations


Joint sessions are planned with Symposium I: "Polycrystalline Thin Films III:
Structure, Texture, Properties, and Applications", and Symposium P:
"Science and Technology of Semiconductor Surface Preparation"

A partial list of invited speakers:
David Clarke (UCSB)
W.W. Gerberich (University of Minnesota)
Qing Ma (Intel)
J. Meindl (Georgia Tech University)
Wayne Paulson (Motorola)
Klaus Schuegraf (Micron)
M. Thouless (University of Michigan)
A.H. Verbruggen (Delft University)
W.L. Brown (Bell Laboratories)
J.E. Greene (University of Illinois)
C.V. Thompson (MIT)


Abstract information can be accessed through the MRS Website:

http://www.mrs.org/meetings/spring97/cfp/

Click on "abstract submission guidelines".

For further information, please contact Robert Keller (address, phone,
email given below).


==============================================================================
Robert R. Keller
National Institute of Standards and Technology
Materials Reliability Division, 853 office: (303)497-7651
325 Broadway FAX: (303)497-5030
Boulder, CO 80303 keller-at-boulder.nist.gov






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Mon, 7 Oct 1996 21:42:16 -0800
Subject: Re: EM Safety Book

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Dear Jacob,
The second edition was published a few years ago and should be available
from the San Francisco Press. I think it is about $16.
Jacob Bastack wrote:
} Has the second edition of the Electron Microscopy Safety Handbook been
} published?
Luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: mager-at-unixg.ubc.ca (Mary Mager)
Date: Mon, 7 Oct 1996 22:11:59 -0800
Subject: Re: Microscopic detection of Cu?

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X-Sender: mager-at-pop.unixg.ubc.ca
Message-Id: {v01510103ae7fa07416b1-at-[137.82.220.6]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Dear Jon,
I think your only choice would be WDX, which has a lower detection limit
than EDX. The sample, however, must be flat. A dressed block, such as is
used to make ultramicrotome samples, may be suitable after carbon coating.
Luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng, UBC
309 - 6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel: 604-822-5648, fax: 604-822-3619
email: mager-at-unixg.ubc.ca






From: Paolo Ghiara :      ghiara-at-sienanet.it
Date: Tue, 08 Oct 1996 09:08:44 -0700
Subject: sub-unsub...

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Message-Id: {325A7C8C.1141-at-sienanet.it}

Goodmornig to everybody,

all the people who want to subscribe or unsubcribe SHOULD send this
message to:


LISTSERVER-at-MSA.Microscopy.Com


OK?

Paolo




From: H.BRINKIES -SE108/TEL.8657 :      hbrinkies-at-swin.edu.au
Date: Tue, 8 Oct 1996 16:18:36 EST+10
Subject: Wax in SEM

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Message-Id: {199610080618.AA14010-at-lucy.swin.edu.au}
Comments: Authenticated sender is {hbrinkies-at-gpo.swin.edu.au}

I have been approached by one of our PhD students to examine the
surfaces of very small metal particles that are mounted in wax molds.
However, I have never used the SEM for wax inpregnated specimens.
Any advise is welcome.
I only have two convential SEMs in my laboratory, and the preparation
facilities are set up for metallographical and geological specimens,
i.e. no cold stages and only a carbon evaporator.

Thanking you

Hans Brinkies
Industrial Microscopy
School of Mechanical and Manufacturing Engineering
Swinburne, University of Technology
Hawthorn - Australia




From: DVCCO-at-aol.com
Date: Tue, 8 Oct 1996 02:46:52 -0400
Subject: Web/PCI Boards/Cameras 10 bit!

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***************************************************
Web site http://www.edt.com/dvc/dvc.html
***************************************************
DVC Company wishes to offer it's web site in a educational manner. The
detailed question and answer section will help researchers in their knowledge
on how to choose a CCD camera, what to look for and what benefits a digital
output & high signal to noise cameras offer..

Does your camera give you the 8 bits you think your getting or would you like
to have 1024 levels of gray to work with in real time. Gray level resolution
is just as important as spacial res!

We offer (camera)(custom digital cable)(framegrabber)(software)

We take the guess work out of picking a PCI bus board/ frame grabber/
digitizer for your Mac or PC. We carry many boards so as to offer a broad
choice for the best in performance relative to the researchers parameters
(all from one source!)
DVC is a U.S. Camera Manufacturer and offers many hardware /software
packages. See boards & camera specifications below.

DVC Cameras- US Manufacturer of a line of monochrome 10 bit analog and
digital cameras offering real time and simultaneous outputs of RS-422 and or
RS-170 video, and upgradeable later.
Frame Grabber Boards - analog or digital for PCI bus for PC and Mac/ others.
Other bus structures available. PCI most popular and fastest.
----------------------------------------------------------
Frame Grabber/ Digitizer List
Matrox 10 bit analog or 10 + bit digital (Pulsar) and others.
Mutech 10 bit analog or 10+ bit digital
Dipix / C40 DSP boards
Coreco
PDI/Precison Digital Imaging for Mac PCI and Mac Nu Bus Boards
Bitflow
ITI/ IMAGING TECHNOLOGY
EDT for S-bus

Tuneable LCD filters
CRI - for RGB sequencial and monochrome 400-1100nm range
for monochrome band pass filter 400-750 nm or 750-1100nm

Software:
Siganl Analytics - IP lab for Mac-PCI and Mac Nu-bus
Noesis Vision
General Imaging
Matrox C library for Win 95 & NT & Inspector-32 IA software
Vaytek / deconvolving microscope images
//////////////////////////////////////////////////////////

MONOCHROME DIGITAL CCD CAMERAS BRING TRUE
10 BIT PRECISION (} 62dB SNR)
TO REAL- TIME IMAGING

DVC-10, DVC-08: 10 or 8 bit RS-422 digital video output with simultaneous
analog RS-170 output.
DVC-0A: SNR } 62dB -at- 0.5 lux (100 IRE) analog
RS-170 output, resolvable to 10 bit precision.
The higher SNR offers increased bit preservation when gain is added for more
sensitivity.
DVC-0A & DVC-08 can be upgraded to DVC-10.
755 (H) X 484 (V) X 30 Hz; 1024 gray levels without external cooling!
Precision opto-mechanical CCD interface for optical stability and
repeatability. 1/2" optical format with industry-standard C-mount.
Contiguous pixels with 100% fill factor, noise floor of 20-25 electrons
typical, & no dead pixels!
Sensor faceplate removal for laser work with 200-1100nm spectral response and
no fringes!
On-camera digitization using CCD pixel clock eliminates pixel jitter &
improves repeatability & sub-pixel accuracy.
Cost effective solution for high end scientific/medical imaging, laser,
interferometry, sub-pixel interpolation & x-ray imaging.

Call DVC for complete imaging solutions including cameras, frame grabbers,
DSP boards, custom cables and software for PCI, Mac, Sun bus & SGI platforms!

DVC is a one-stop source, offering plug-and-play systems. Custom options
available for OEM and other applications

DVC Company, San Diego, CA
Tel: (619) 444-8300 DVC Cameras are
Fax: (619)444-8321 made in the USA.
e-mail: dvcco-at-aol.com Two year warranty.
www.edt.com/dvc/dvc.html





From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Tue, 8 Oct 1996 09:07:16 +0100
Subject: Re: Microscopic detection of Cu?

Contents Retrieved from Microscopy Listserver Archives
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X-Sender: (Unverified)
Message-Id: {v01510101ae7faeba9494-at-[158.152.199.245]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Greetings:
}
} I am trying to help a user of our lab solve a problem involving locating
} and quantifying small concentrations of copper in plant cells.
}
} Here is the setup: Small plants are grown in soil high in copper. These
} plants accumulate copper in the trichomes (hairs) of the plant ( we think).
}
} Here is the question: How can it be demonstrated that copper is actually
} accumulated in the trichomes and how closely can we approximate its
} concentration?
}
} I have tried helping her by doing some quick and dirty SEM/EDS. I could not
} detect any copper in the trichomes (maybe I wasn't looking hard enough).
} She reports that there should be around 1% copper or less per dry weight of
} tissue. I suggested that that amount maybe hard to pin down using EDS, but
} I told her I would check it out with the experts to see what a reasonable
} minimum detectable amount might be (OK experts, what do you think?).
}
} I also cautioned her regarding the pitfalls of EDS on biological samples,
} suggesting that she may want to explore alternatives to her preparation
} technique. She is currently fixing and embedding in paraffin so she can
} section for the light microscope. If freezing and other cryo techniques are
} the best way to go, I could use some help (and back-up) in advising her of
} how far to go and the chance of success. Is simple freezing enough or does
} she have to find someone with a cryo stage to keep everything in place
} throughout the analysis?
}
} Finally, do you think there is any future in this type of investigation? If
} so, do you have any ideas of where we might be able to find the equipment
} needed to proceed? If not, do you have any alternatives you could share?
}
} Perhaps I am too pessimistic, maybe I don't know what I am talking about.
} Either way it would help us to get some outside opinions and ideas about
} this problem.
}
} Thanks,
}
} Jonathan Krupp
} Microscopy and Imaging Lab
} University of California
.
.
} Here is an update on the Cu concentration in the plant trichomes. Latest
} bulletin from trichome central is that the concentration is probably closer
} to 0.1% Cu.

} Now that's a horse of a different color!

Jonathon,

Even with the revised concentration estimate it MAY be possible to do by EM
and possibly (?!) even without cryo.

Two key questions:

1. Is the Cu evenly distributed or concentrated in specific sights? If
evenly distributed, then you have a difficult problem. If concentrated in
very specific sights, you may have a somewhat easier problem. i.e. if it is
0.1wt% everywhere, you have a problem but the Cu may be concentrated in the
surface layer of the hairs, then the LOCAL concentration could be much
higher 1wt% - 5wt%.

So can you use knowledge of the metabolic routes involving the Cu to guess
where it might be? Alternatively, BSE in the SEM or a STEM, or just high
contrast in an unstained TEM section might indicate regions to check for
Cu.

2. What makes you think that the Cu is still in the specimen? Unless you
KNOW that the Cu has been immobilised, most specimen preparation procedures
are not designed to fix elements but structure. So, unless I new different,
I would assume that the Cu was washed out of the specimen during
preparation. Although Cu does tend to be fairly immobile - it's not like Na
or Ca.

IF the CU is immobilised AND locally concentrated, you stand a chance. but
I would guess you still need to do something about the specimen
preparation. Not being an expert in this area, I would suggest that you
need to follow more standard EM preparation techniques but try to avoid all
heavy metals (glutaradehyde fixation?) - their presence will mask small
amounts of Cu. Of coure, this means that even if you do find the Cu, you
may not be able to determine where it is in relation to the anatomy of the
specimen!.

You also need to avoid coating the specimen - Au or C. In the SEM, this
will probably mean low kV to minimise specimen charging but trying to pick
up the Cu L peak will be difficult - high X-ray background at low energies
- and you won't be able to go lower than about 2.5 keV anyway, or you'll
never excite the Cu L peak. To excite the Cu K peak you'll need to be over
12keV and preferably around 20keV. All of this tends to suggest that you
stand a better chance in a TEM.

More generally, if the Cu is mobile and/or not localised, to stand a chance
of getting a result via EM, you will certainly need to follow a cryo route.
This means freezing the specimen to fix the Cu in the correct place in the
specimen and then using a cryo-stage to keep it cold.

My preference would be for cryo-TEM. The EDX spatial resolution will be
higher - no beam spreading in a thin specimen - so IF the Cu is locally
concentrated and you can find the right area, your chance of detection will
be better.

A key factor in EDX of cryo-specimens is water content. If there is a lot
of water in the specimen, this will obscure the presence of trace elements
- you will need to dehydrate the specimen inside the EM. You'll probably
need/want to do this anyway to improve resolution and contrast.

If cryo-SEM, don't coat the specimen - even with C and certainly not with
Au or other heavy metals.

And don't forget that Cu is everywhere in most EMs. You need to do a
careful check that any Cu you do pick up is really coming from the specimen
- particularly in a TEM - e.g. Cu specimen grids, specimen holders (brass?
and/or Cu parts), cold traps, an so on.

Also, I'd recommend a thorough literature search.

Hope that is some help - best of luck!

Regards,

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: Larry-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
---------------------------------------------------------------






From: Eddy Robinson :      74260.1673-at-CompuServe.COM
Date: 08 Oct 96 17:52:25 EDT
Subject: SPM/NSOM Workshop October 22nd

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14TH INTERNATIONAL SPM WORKSHOP
INTERNATIONAL WORKSHOP ON NEAR-FIELD SCANNING OPTICAL
MICROSCOPY

Tuesday, October 22, 1996
8:00 a.m. to 6:00 p.m.
Stanford University
Stanford, California

International Workshop On Near-field Scanning Optical Microscopy
Near-field Scanning Optical Microscopy (NSOM) allows the extension of optical
microscopy, spectroscopy, and other photonic techniques beyond the Abbe barrier,
also known as the diffraction limit. Recent advances in Scanning Probe
Microscopy have made NSOM, first postulated in 1925, into a reality.

This workshop will showcase new applications and trends in NSOM and allow
in-depth discussion with some of the world's leading practitioners of the
technique.

NSOM HAS APPLICATIONS IN ALL AREAS OF SCIENCE

Based on classical optical contrast and providing unique information on
electronic
properties of matter, NSOM has potential applications in all areas of research.
Speakers are drawn from groups interested in practical applications of NSOM to
areas which are limited by conventional optical resolution. Areas of discussion
will
range from biology and clinical research to semiconductor technology and
materials science. All invited participants are keenly interested in developing
new areas of application for NSOM.

WHO SHOULD ATTEND

Because the workshop will aim to spark discussion of both exciting and novel
NSOM applications, anyone with any interest in Scanning Probe Microscopy or
anyone who would like to extend existing light-based studies to higher
resolution
should attend.

POSTER SESSION

Anyone with research in the field of NSOM or more general areas of Scanning
Probe Microscopy is encouraged to submit a poster. Display facilities will be
available during morning and afternoon breaks and during the lunch-time
discussion period.

HOW TO REGISTER

Please complete the registration form (page 6) and return it to TopoMetrix
Corporation in Santa Clara, California. This registration fee covers the full
day*s
attendance including eight speakers, lunch, and refreshments throughout the
workshop.


AGENDA

8:00 - Registration
8:45 - Welcome address by Professor Ronald Hanson, Department Chairman,
Department of Mechanical Engineering, Stanford University.
9:00 - Mark Utlaut, University of Portland - Introduction to Near-field Scanning

Optical Microscopy
9:30 - Philip Haydon, Iowa State University - Opportunities for Near-field
Microscopy in Biology
10:10 - Break
10:40 - Wolfgang Heckl, University of Munich - Scanning Probe Microscopy of
DNA from Molecules to Chromosomes
11:20 - Shyamsunder Erramilli, Boston University - Scanning Near-field Infrared
Microscope (SNIM) Based on a Free Electron Laser
12:00 - Lunch/Poster Session
1:30 - Kenneth Goodson, Stanford University - Laser Thermometry and Processing
in the Near-field
2:10 - Walter Duncan, Texas Instruments - Near-field Optical Spectroscopy of
Electronic Materials and Structures
2:50 - Break
3:20 - Nigel Cave, Motorola Corp. - Near-field Optical Microscopy and
Spectroscopy of
Semiconductors
4:00 - Ludwig Balk, University of Wuppertal, Germany - Device Characterization
and Failure Analysis by Means of Near-field Scanning Optical Microscopy
4:40 - Final Discussion

BIOGRAPHIES:

PROFESSOR LUDWIG BALK
Dr. Balk studied physics at the Technical University of Aachen, gaining his
diploma
in 1971, then his doctoral degree within the faculty of electrical engineering
in
1976. Following his move to Duisberg University as Academic Director in the
Department of Materials, Dr. Balk took a full chair professorship for
electronics in
the faculty of electrical engineering at the University of Wuppertal in 1991 and
is
presently dean of faculty. His applications range from pure electronic materials
to
inorganic materials, such as functional ceramics, and biological materials, such
as
human tissues and cells.

DR. NIGEL CAVE
Dr. Cave is a senior staff scientist in Material Research and Strategic
Technologies at
Motorola. He uses optical techniques to characterize new semiconductor materials

and processes. He is currently developing near-field optical techniques within
Motorola to permit deep sub-micron analysis of semiconductor devices. Dr. Cave
received his Ph.D. from Imperial College, London in 1990 and did his
post-doctoral
work at the University of Cincinnati.

DR. WALTER DUNCAN
Dr. Duncan is a senior member of the technical staff in the Materials Science
Laboratory of Texas Instruments. He received his B.S. in Chemistry from Virginia

Polytechnic Institute in 1974 and his Ph.D. in Inorganic Chemistry from North
Carolina State University in 1979. His current research is directed towards in
situ
wafer state sensors for adaptive control of vacuum processes, near-field optical

microscopy, optical properties of quantum confined structures, and silicon based

optoelectronic integrated circuits.

DR. SHYAMSUNDER ERRAMILLI
Dr. Erramilli is a professor in Photonics and Physics at Boston University,
granted a
joint appointment in 1996. He received his Ph.D in Physics from the University
of
Illinois working with Hans Frauenfelder. He later joined the faculty of the
Physics
department at Princeton University, where he taught for ten years. His research
interests have included the application of imaging techniques in Biological
Physics,
using x-ray diffraction and high pressure to study biological systems. Most
recently,
Dr. Erramilli has worked in the development of new forms of infrared microscopy.

In 1995, he was named a recipient of the duPont Young Professor award.

PROFESSOR KENNETH GOODSON
Dr. Goodson is a professor and Terman Fellow with the Mechanical Engineering
Department at Stanford University. He received a Ph.D. from M.I.T. in 1993 and
was visiting scientist for two years with Daimler-Benz AG. His research
investigates
thermal phenomena in semiconductor devices and interconnects for integrated
circuits. Dr. Goodson's group is using NSOM to improve the spatial resolution of

far-field optical thermometry and thermal processing techniques developed
previously in his laboratory. He is an Office of Naval Research Young
Investigator
and a recipient of the National Science Foundation CAREER Award.

PROFESSOR PHILIP HAYDON
Dr. Haydon is a professor and the director of the Signal Transduction Training
Group as well as the Laboratory of Cellular Signaling at Iowa State University.
His
research focuses on ion channels and synaptic trans-mission between neurons. He
is
applying AFM and NSOM techniques to these problems.

PROFESSOR WOLFGANG HECKL
Dr. Heckl is a professor in Experimental Physics at the University of Munich in
the
Institute for Crystallography & Mineralogy. Dr. Heckl gained his Physics diploma
at
Munich in 1985 followed by his Ph.D. in experimental physics under Professors
Moewald and Sackmann. Post-doctorate positions followed at the Max-Planck
Institute and the University of Toronto before working with Professor G. Binnig
at
IBM Research (STM) and Prof. T. Hansch at the Ludwig-Maximilians University
Munich (STM/SFM/NSOM).

PROFESSOR MARK UTLAUT
Dr. Utlaut is a professor in the Department of Chemistry and Physics at the
University of Portland where he pursues research in the applications of Focused
Ion
Beam and Secondary Ion Mass Spectrometry technology as well as NSOM
imaging. Dr. Utlaut received his B.S. in Physics from the University of Colorado
and
his Ph.D. in Physics from the University of Chicago.


REGISTRATION
HOW TO REGISTER
Complete the advanced registration form below and return with your check or
money order. Payment must be included in order to process your registration.
On-site registration will be available on a first-come, first-serve basis only.
All
workshop registrations received by October 10, 1996 will be confirmed. Badges
will
be available at the workshop registration area on the day of the workshop.
Registration begins at 8:00 a.m.

CANCELLATION AND REFUND POLICY
If you need to cancel for any reason, you must notify TopoMetrix Corporation, in

writing, by 5:00 p.m., October 15, 1996 to receive a full refund. Because the
tickets may sell out before the workshop, refunds will not be granted on
cancellations received after that date.

SPEAKER AVAILABILITY
The speakers listed in this brochure are leading professionals in their
respective
fields. Should a speaker be unable to attend the workshop, all efforts will be
made
to replace that speaker with one of comparable experience and qualifications.

WORKSHOP REGISTRATION:
FAX to:
408/982-9751

MAIL to:
TopoMetrix Corporation
Attention: Workshop Registration
5403 Betsy Ross Drive
Santa Clara, CA 95054

For Workshop Information call: 408/982-9700-Jan McNerney

COST:
Industry scientists, executives and academics: $50.00
Students, interns and lab technicians: $20.00

PLEASE SEND THE FOLLOWING INFORMATION:

First Name, Middle, Last Name

Title/Department, Company

Address

City, State/Prov, Zip/Postal Code

Phone, Facsimile

Registration must be payed before the Workshop.

Enter total payment due: $_____

Check or money order must be made payable to "TopoMetrix Corporation".





From: slakmon-at-scottscientific.com (P. Slakmon)
Date: Tue, 8 Oct 1996 20:18:53 -0400 (EDT)
Subject: Polaroid Equipment & Supplies

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We invite and welcome anyone who is interested in visiting & seeing all of
Polaroids equipment and supplies in Montreal, Quebec, Canada on October 9 & 10.

Anyone interested in a visit through our Polaroid Expo, please respond ASAP
to polaroid-at-scottscientific.com


Scott Scientific
_______________________________________________
SCOTT SCIENTIFIC
P.O. Box 66552 Station Cavendish
Montreal, Quebec, H4W 3J6, Canada

Telephone: 514-485-2309 Fax: 514-485-9931
Voice Mail: 514-888-6509

WWW Site: http://www.scottscientific.com

E-Mail: info-at-scottscientific.com
sales-at-scottscientific.com
admin-at-scottscientific.com
links-at-scottscientific.com
_______________________________________________





From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: 10/8/96 4:18 PM
Subject: Wax in SEM

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Mime-Version: 1.0
"H.BRINKIES -SE108/TEL.8657" {hbrinkies-at-swin.edu.au}
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Hans;

Assuming that the metal particles of interest actually protrude from the
wax surface (and are not coated with wax), you should be able to image
these samples and perhaps even do some EDS work on them if they are large
enough (i.e. minimum of 1 micron). A thermal evaporated carbon film of
about 10-15 nm should be sufficient for conductive purposes, however I
would also paint a very thin trail of carbon dag from the surface of the
sample along the edge to the SEM stud. You may have to image at lower kV's
(10 or perhaps even 5 kV). If you find the sample still charges a little,
try going down maybe 1kV and leave it in the same location for a few
minutes. Sometimes you can balance the charge buildup and dissipation this
way.

If your microscope has an auto brightness control, switch to manual
control; and if you have digital imaging on the SEM, this will be a
tremendous help as well in reducing the amount of charge observed in your
final image, because images can be captured quickly.

Hope this is useful. If not, pretend I didn't write it.

Regards,

-Bob
******************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA 91711
ph: (909)399-1311
email: Bob_Citron-at-cc.chiron.com
*******************************
______________________________ Reply Separator _________________________________


I have been approached by one of our PhD students to examine the
surfaces of very small metal particles that are mounted in wax molds.
However, I have never used the SEM for wax inpregnated specimens.
Any advise is welcome.
I only have two convential SEMs in my laboratory, and the preparation
facilities are set up for metallographical and geological specimens,
i.e. no cold stages and only a carbon evaporator.

Thanking you

Hans Brinkies
Industrial Microscopy
School of Mechanical and Manufacturing Engineering
Swinburne, University of Technology
Hawthorn - Australia




From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: 10/7/96 10:53 AM
Subject: Microscopic detection of Cu?

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Mime-Version: 1.0

Jon:

If I understand the requirements of this assay correctly, it seems to me that
microscopy isn't necessarily the best technique to use in this application. I am
not a biologist, but if you can somehow isolate/section away the trichomes from
the plant tissues, you might be able to perform this analysis by AA (atomic
absorption spectroscopy) or GFAA (graphite furnace AA techniques). Both of
these methods yield detection limits far lower than you will ever get with EDS.
I have performed similar analyses on tissues of marine bivalves with very good
results.

Good luck in your studies;

-Bob
**************************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA 91711
ph: (909)399-1311
email: Bob_Citron-at-cc.chiron.com
**************************************

______________________________ Reply Separator _________________________________


Greetings:

I am trying to help a user of our lab solve a problem involving locating
and quantifying small concentrations of copper in plant cells.

Here is the setup: Small plants are grown in soil high in copper. These
plants accumulate copper in the trichomes (hairs) of the plant ( we think).


Here is the question: How can it be demonstrated that copper is actually
accumulated in the trichomes and how closely can we approximate its
concentration?

I have tried helping her by doing some quick and dirty SEM/EDS. I could not
detect any copper in the trichomes (maybe I wasn't looking hard enough).
She reports that there should be around 1% copper or less per dry weight of
tissue. I suggested that that amount maybe hard to pin down using EDS, but
I told her I would check it out with the experts to see what a reasonable
minimum detectable amount might be (OK experts, what do you think?).

I also cautioned her regarding the pitfalls of EDS on biological samples,
suggesting that she may want to explore alternatives to her preparation
technique. She is currently fixing and embedding in paraffin so she can
section for the light microscope. If freezing and other cryo techniques are
the best way to go, I could use some help (and back-up) in advising her of
how far to go and the chance of success. Is simple freezing enough or does
she have to find someone with a cryo stage to keep everything in place
throughout the analysis?

Finally, do you think there is any future in this type of investigation? If
so, do you have any ideas of where we might be able to find the equipment
needed to proceed? If not, do you have any alternatives you could share?

Perhaps I am too pessimistic, maybe I don't know what I am talking about.
Either way it would help us to get some outside opinions and ideas about
this problem.

Thanks,

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
FAX (408) 429-0146
jmkrupp-at-cats.ucsc.edu






From: rutledge phil :      prutle1-at-umbc.edu
Date: Wed, 9 Oct 1996 10:11:03 -0400 (EDT)
Subject: agarose embedding for immuno staining (fwd)

Contents Retrieved from Microscopy Listserver Archives
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X-Authentication-Warning: umbc7.umbc.edu: prutle1 owned process doing -bs



---------- Forwarded message ----------

} Date: Wed, 2 Oct 1996 15:00:39 -0700 (PDT)
} From: Sarah Bottjer {bottjer-at-alnitak.usc.edu}
} To: neuroethologists {neuroethology-at-sio.ucsd.edu}
} Cc: soumya iyengar {iyengar-at-usc.edu}
} Subject: agarose embedding for immuno staining
} Mime-Version: 1.0
} Status: RO
}
} does anyone out there have a good protocol for embedding tissue in agarose
} (for subsequent frozen sectioning and free-floating immuno reaction)?? We
} prefer not to use gelatin-albumin, since we are embedding small bryozoan
} larvae, and it's a distinct advantage to be able to see them in the
} agarose!
} (p.s. please don't ask me how a songbird lab got involved with
} bryozoans . . .)
} Thanks in advance, Sarah Bottjer
}






From: bettina :      FEDTKE-at-ubaclu.unibas.ch
Date: Wed, 09 Oct 1996 17:14:45 +0100
Subject: TEM - Fourier transform (or optical diffraction) of

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


drift and vibration

It is said, that the FT of drift is different to the FT of
vibration (for example on carbon film only).

The reason for this difference is not clear to me, because in
both cases the "points" on the object are represented on the
image by an elongated shape.

Where does the difference in FT come from, and how does the
difference in FT look like??


------------------------------------------------------------------------
Bettina Fedtke fedtke-at-ubaclu.unibas.ch
Maurice E. Mueller Institute University of Basel, Switzerland
Klingelberstr. 70 Tel +41-61-267 2257
CH-4056 Basel Fax +41-61-267 2259
------------------------------------------------------------------------




From: Heinz Wolff :      Heinz.Wolff-at-brunel.ac.uk
Date: Wed, 9 Oct 1996 16:03:14 +0000
Subject: Re: www

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Message-Id: {199610091559.KAA06323-at-Sparc5.Microscopy.Com}
X-Sender: ibsrhsw-at-mailhost.brunel.ac.uk
X-Mailer: Windows Eudora Light Version 1.5.2
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
To: bjford-at-cix.compulink.co.uk, lct1001-at-hermes.cam.ac.uk, patbob-at-sequent.com,
kwiackow-at-geography.ucl.ac.uk, osp086-at-clss1.bangor.ac.uk,
100443.3474-at-CompuServe.COM, rwilliamson-at-cix.compulink.co.uk,
jefwolf-at-pipeline.com, 100064.1252-at-CompuServe.COM,
72511.447-at-CompuServe.COM, 100437.567-at-CompuServe.COM, sharilee-at-aol.com,
awright-at-uoguelph.ca, Foxvulpes1-at-aol.com, 100424.3727-at-CompuServe.COM,
100332.1673-at-CompuServe.COM, myates-at-citygate.demon.co.uk,
74722.703-at-CompuServe.COM, Microscopy-at-aaem.amc.anl.gov,
ZWANENBU-at-pacific.net.sg

At 07:08 PM 10/4/96 BST-1, Brian Ford wrote:
}
}
} We have a new web page. You will find it on:
}
} www.sciences/demon.co.uk
}
} We have e-mail on demon, too:
}
} brian-at-sciences.demon.co.uk
}
} Let me know what you think of the web page - it's only provisional, I
} shall not really announce it 'til Christmas.
}
} Best wishes,
} Brian J Ford
}
Dear Brian,

If www.sciences/demon.co.uk is the correct address, than I cannot find
it; I have tried on a number of occasions.

Yours,


HEINZ}
}
}





From: Franklin Bailey :      jfb-at-novell.uidaho.edu
Date: Wed, 9 Oct 1996 11:45:34 PST8PDT
Subject: unsubscribe

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Please unsubscribe for now




From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Wed, 9 Oct 1996 11:27:48 -0500 (EST)
Subject: Re: agarose embedding for immuno staining (fwd)

Contents Retrieved from Microscopy Listserver Archives
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I do not think agarose freezes very well. The water tends to separate from
the colloid and makes large ice crystals. At least this is what happens
during slow freezing. On thawing the agrose then sorta precipitates out
leaving free water. Way back when, we used to recycle agarose for
microbiological culture and that is how we separated the agarose from the
soluble components. I am trying to think of an insoluble alternative that
might work. Maybe polyacrylamide
}
} ---------- Forwarded message ----------
}
} } Date: Wed, 2 Oct 1996 15:00:39 -0700 (PDT)
} } From: Sarah Bottjer {bottjer-at-alnitak.usc.edu}
} } To: neuroethologists {neuroethology-at-sio.ucsd.edu}
} } Cc: soumya iyengar {iyengar-at-usc.edu}
} } Subject: agarose embedding for immuno staining
} } Mime-Version: 1.0
} } Status: RO
} }
} } does anyone out there have a good protocol for embedding tissue in agarose
} } (for subsequent frozen sectioning and free-floating immuno reaction)?? We
} } prefer not to use gelatin-albumin, since we are embedding small bryozoan
} } larvae, and it's a distinct advantage to be able to see them in the
} } agarose!
} } (p.s. please don't ask me how a songbird lab got involved with
} } bryozoans . . .)
} } Thanks in advance, Sarah Bottjer
} }
}
}
}
}
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*******************************************************





From: Mark Zirinsky - Production Engineering :      markz-at-pemed.com
Date: Wed, 09 Oct 1996 14:48:07 -0700
Subject: Phillips Transmission Electron Microscope

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Message-ID: {325C1D97.7D2D-at-pemed.com}

We have a Philips TEM CM-12 for sale.

We would appreciate hearing from anyone who has experience operating
this instrument.

http://www.pemed.com/newdock/newdock.htm

or e-mail to :

marz-at-pemed.com




From: Mark Zirinsky - Production Engineering :      markz-at-pemed.com
Date: Wed, 09 Oct 1996 14:51:02 -0700
Subject: Re: Phillips Transmission Electron Microscope

Contents Retrieved from Microscopy Listserver Archives
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Message-ID: {325C1E46.7528-at-pemed.com}

We have a Philips TEM CM-12 for sale.

We would appreciate hearing from anyone who has experience operating
this instrument.

http://www.pemed.com/newdock/newdock.htm

or e-mail to :

markz-at-pemed.com




From: bettina
Date: 10/9/96 1:52 PM
Subject: TEM - Fourier transform (or optical diffraction) of

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Message-Id: {n1367251534.36536-at-macmail.lbl.gov}

Reply to: RE} TEM - FT of drift and vibration

Bettina:

Both drift and vibration will smear out image points into line segments. The
image power spectrum will then be truncated in the direction of the drift or
vibration. How can we distinguish between drift and vibration in the power
spectrum?

Theoretical answer:
For an exposure time that is longer than several vibration periods, vibration
smear will have a Gaussian-like profile. Drift smear will be constant over
the exposure with an abrupt step at each end of the line segment. The FTs of
these functions will be different; one will produce a reciprocal-space
damping function with a Gaussian-like profile, whereas the other will have a
sin(x)/x profile showing a banded ringing effect.

Practical answer:
A series of images taken with increasing exposure times will show the same
truncation of the power spectrum (FT) for vibration, but an increasing effect
for drift (assuming the drift is fairly constant).

Mike O'Keefe
--------------------------------------
drift and vibration

It is said, that the FT of drift is different to the FT of
vibration (for example on carbon film only).

The reason for this difference is not clear to me, because in
both cases the "points" on the object are represented on the
image by an elongated shape.

Where does the difference in FT come from, and how does the
difference in FT look like??


------------------------------------------------------------------------
Bettina Fedtke fedtke-at-ubaclu.unibas.ch
Maurice E. Mueller Institute University of Basel, Switzerland
Klingelberstr. 70 Tel +41-61-267 2257
CH-4056 Basel Fax +41-61-267 2259
------------------------------------------------------------------------
:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:
Michael A. O'Keefe, Deputy Head
National Center for Electron Microscopy
Lawrence Berkeley National Laboratory
University of California
Berkeley, California 94720
tel: (510) 486-4610
fax: (510) 486-5888
email: maok-at-lbl.gov
http://ncem.lbl.gov/
:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:






From: bozzola-at-siu.edu (John J. Bozzola)
Date: Wed, 9 Oct 1996 11:20:54 -0600
Subject: MIKMAS/CSMS Meeting Program

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If you are interested in attending this joint meeting, contact me by e-mail
or check out our website at:

http://www.cvm.uiuc.edu/Homepages/LouAnnMiller/CSMS/csms.html

PROGRAM


8:00-8:30 Registration, Vendor Displays and Welcome Coffee
at Jesse Wrench Auditorium.

8:30-8:35 Welcome and comments.

8:35-8:55 Don Parker, Monsanto, St. Louis.
"Network Interfacing of Microscopes"

8:55-9:15 Lee Dickey, Micro Lines Marketing, St. Louis.
"Ultramicrotomy in Material Sciences"

9:15-9:35 Nancy Wilson Rizzo, SemTech, Inc, Shawnee Mission,
Kansas.
"Analysis of Gunshot Residue"

9:35-10:05 Phil Fraundorf, Physics and Astronomy, University
of Missouri-St. Louis.
"Some Developments in Air-Based Scanning Tunneling
Microscopy"

10:05-10:30 Morning Break-Refreshments.

10:30-11:30 Dale E. Newbury (MAS Tour Speaker) National
Institute of Standards
and Technology, Gaithersburg, Maryland.
"Lies, Damned Lies, and Standardless Analysis"

11:30-1:00 Lunch at Glen's Cafe: $10/person. Cajun/southwest
cuisine: salad,
brisket, shrimp creole, chicken, veggies, potatoes,
cornbread, drinks.


R.S.V.P. -- Lou Ross, (573) 882-4777
or geosclmr-at-showme.missouri.edu
or Fax (573) 882-5458
by Tuesday, October 15th!

1:00-1:20 Heidi Schatten, Veterinary Sciences, University of
Missouri-Columbia.
"Cytoskeletal Organization during Fertilization, Cell Division, and
Development in
Microgravity: Results from a Space Flight (STS-77) on the Shuttle
Endeavor"

1:20-1:40 Tobias Baskin, Biological Sciences, University of
Missouri-Columbia.
"Cryofixation of Large Samples for Light Microscopy"

1:40-2:00 Hari B. Krishnan, Plant Pathology, University of
Missouri-Columbia.
"Immunocytochemical Localization of Storage Proteins in Developing
Rice Endosperm"

2:00-2:20 John Burton, Cardiology, University of Missouri-Columbia.
"Utilization of Confocal Laser Microscopy and Image Analysis
as Tools in Medicine Today"

2:20-2:35 Afternoon Break-Refreshments

2:35-2:55 James W. Cogswell, L.M. Ross Jr., M.J. O'Brien, H. Neff,
and M.D. Glascock,
Anthropology and Geological Sciences, University of Missouri-Columbia.
"Postmanufacture Effects on the Chemical Characterization of
Prehistoric Pottery:
Evidence from the Central Mississippi River Valley"

2:55-3:15 Robert R. Church, Anthropology, University of Missouri-Columbia.
"The Effect of Boiling on Osteological Materials"

3:15-3:35 Rajat Roychoudhury, E.J. Charleson, and L.M. Ross, Jr,
Electrical Engineering
and Geological Sciences, University of Missouri-Columbia.
"Microcharacterization of Phosphorus Doped Diamond Thin Film
Interfaces Using
Voltage Contrast and Electron Beam Induced Current Studies"

3:35-3:55 Fariborz Golshani, M. Prelas, L.M. Ross, Jr., Nuclear
Engineering and Geological
Sciences, University of Missouri-Columbia and John L. Fitz,
University of Maryland.
"CL Study of Polycrystalline Diamond Films Before and After Annealing"

4:00- Business Meetings

####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: wise-at-vaxa.cis.uwosh.edu
Date: Wed, 09 Oct 1996 18:14:51 +0000
Subject: Poly-L-lysine

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To all,

I have never worked with poly-L-lysine coated slips (but soon will)
and have a couple of questions. It looks like a fairly easy procedure but
none of the several dozen EM books on my shelf say much about it.

1. What is the shelf life of an aqueous solution of poly-L-lysine?
2. What is the shelf life of coated cover slips and how should they be stored?
3. When making lysine coated cover slips, the procedure (as I understand
it) is to cover a cover slip with an aqueous solution of poly-L for one
hour then dab dry. Can that drop of solution be transferred to a fresh
cover slip or has its supply of lysine been exhausted? Do slips need to be
cleaned in any particular fashion?
4. When working with cells in culture, is it better to stick them to the
cover slips as a living culture or after glut or osmium treatment?
5. If living cells are exposed to a coated cover slip, does subsequent
glutaraldehyde treatment cross link the cells to the lysine and increase
adhesion?
6. How long should the cell culture be left on the coated cover slip?
Temperature?
7. What density of cells in the culture works best?
8. Any special concerns during dehydration, CPD, or sputter coating?
9. Did I leave anything out?

Thanks in advance
Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-uwosh.edu






From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 9 Oct 1996 16:42:17 -0400 (EDT)
Subject: Re: TEM - FT of drift and vibration

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} It is said, that the FT of drift is different to the FT of
} vibration (for example on carbon film only).
}
} The reason for this difference is not clear to me, because in
} both cases the "points" on the object are represented on the
} image by an elongated shape.
}
} Where does the difference in FT come from, and how does the
} difference in FT look like??
}
Dear Bettina,
Assume for the moment that the vibration has constant amplitude,
the drift is always in the same direction and the exposure time for the
image is long compared to the period of the vibration. In this case, the
image corresponding to a point is a line segment which is of fixed length
in the case of a vibration and which is proportional to the exposure time
for drift. Furthermore, if the vibration is from simple harmonic motion,
the intensity along the line segment is greater at the ends than in the
middle; whereas, the intensity for drift is constant for constant drift
velocity. I won't go into the details of the math, but there will be a
large Fourier amplitude for the spatial frequency corresponding to the
amplitude of the vibration or for the distance of the drift. There will
be differences in the higher-frequency Fourier amplitudes depending on
whether the intensity along the line segment is variable (vibration) or
constant (drift), and for a series of different exposure times, these
large amplitudes will occur at the same (vibration) or different (drift)
frequencies.
That said, in the real world, vibrations do not have constant
amplitude, drift is not unidirectional and, depending on the source,
exposure times are not necessarily longer than vibration periods, al-
though they usually are. Another consideration for distinguishing drift
from vibration by taking a series of exposures of different lengths is
that the drift may be induced by the beam and may vary with the beam
conditions. By spreading the beam to get the proper illumination for
a longer exposure, you might slow the drift, so that the line segment
produced will be the same length on the film.
The easiest way to see what the differences in the FT look like
is to rotate the image so that the axis of the line segments is along
the x- or y-axis and do a 1-D FT; i.e. multiply the intensity by cos(2pi
hx/l) and integrate over x, where l is the length of the image. This will
give F(h) with the peaks as described above. The line segments should be
uncorrelated, so the Fourier amplitudes from each should add with random
phase, and the total amplitude distribution should look like that from a
single line segment.
Yours,
Bill Tivol




From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Wed, 9 Oct 1996 18:47:25 -0500
Subject: MMMS Meeting-Oct 29 on Microscopy in HealthCare

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Message-Id: {v03007803ae81e8aee2c2-at-[206.69.208.21]}
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Content-Transfer-Encoding: quoted-printable

{fontfamily} {param} Times_New_Roman {/param} Midwest Microscopy and
Microanalysis Society
Meeting {/fontfamily} {fontfamily} {param} Times {/param}


{/fontfamily} {fontfamily} {param} Times_New_Roman {/param} Microscopy: A
Problem - Solving Tool=20

in the Healthcare
Industr {/fontfamily} {fontfamily} {param} Times {/param} y

{/fontfamily} {fontfamily} {param} Times_New_Roman {/param}

Tuesday, October 29,
199 {/fontfamily} {fontfamily} {param} Times {/param} 6 {/fontfamily} {fontfamily} {p=
aram} Times_New_Roman {/param}

Abbott
Laboratorie {/fontfamily} {fontfamily} {param} Times {/param} s {/fontfamily} {fontf=
amily} {param} Times_New_Roman {/param}

Abbott Park, Illinoi {/fontfamily} {fontfamily} {param} Times {/param} s


Additional Information will be posted on the MMMS WWW Site


http://www.msa.microscopy.com/MSALAS/MMMS/MMMSHomePage.html

{/fontfamily} {fontfamily} {param} Times_New_Roman {/param}

12:00 - 1:00 Lunch (provided by Abbott
Laboratories {/fontfamily} {fontfamily} {param} Times {/param} )

{/fontfamily} {fontfamily} {param} Times_New_Roman {/param}

1:00 - 1:15 Welcom {/fontfamily} {fontfamily} {param} Times {/param} e

{/fontfamily} {fontfamily} {param} Times_New_Roman {/param}

1:15 - 2:00 Pharmaceutical Sleuthing - Applications of Light
Microscop {/fontfamily} {fontfamily} {param} Times {/param} y {/fontfamily} {fontfam=
ily} {param} Times_New_Roman {/param}

Scott Aldrich, Trace Substance Analysis, Pharmacia & Upjohn, Inc.;
Kalamazoo M {/fontfamily} {fontfamily} {param} Times {/param} I

{/fontfamily} {fontfamily} {param} Times_New_Roman {/param}

2:00 - 2:45 Imaging Applications in the Pharmaceutical Industry: Not
Just Pretty
Picture {/fontfamily} {fontfamily} {param} Times {/param} s {/fontfamily} {fontfamil=
y} {param} Times_New_Roman {/param}

Michail Esterman, Technology Core Research, Lilly Research
Laboratories, Indianapolis
I {/fontfamily} {fontfamily} {param} Times {/param} N

{/fontfamily} {fontfamily} {param} Times_New_Roman {/param}

2:45- 3:15 Brea {/fontfamily} {fontfamily} {param} Times {/param} k

{/fontfamily} {fontfamily} {param} Times_New_Roman {/param}

3:15 - 4:00 Electron Microscopy in Pathology: Under the Regulatory
Microscop {/fontfamily} {fontfamily} {param} Times {/param} e {/fontfamily} {fontfam=
ily} {param} Times_New_Roman {/param}

Sandy L. White and Jeffrey W. Horn, Toxicology Research Laboratories,
Lilly Research Laboratories, Greenfield
I {/fontfamily} {fontfamily} {param} Times {/param} N

{/fontfamily} {fontfamily} {param} Times_New_Roman {/param}

4:00 - 4:45 Microscopy in Medical Product
Developmen {/fontfamily} {fontfamily} {param} Times {/param} t {/fontfamily} {fontfa=
mily} {param} Times_New_Roman {/param}

Jim DiOrio, Medical Materials Technology Center, Baxter Healthcare
Corporation, Round Lake
I {/fontfamily} {fontfamily} {param} Times {/param} L

{/fontfamily} {fontfamily} {param} Times_New_Roman {/param}

4:45 Tour of the Department of Microscopy and
Microanalysi {/fontfamily} {fontfamily} {param} Times {/param} s

{/fontfamily} {fontfamily} {param} Times_New_Roman {/param}

To register, please contact Jerry Gagne by October 11=20

phone (847-938-5023), FAX (847-938-5027) or E-mail
(gerard.d.gagne-at-abbott.co {/fontfamily} {fontfamily} {param} Times {/param} m) {/fo=
ntfamily} {fontfamily} {param} Times_New_Roman {/param}


Please help us out by registering ahead of time! The main security
gate at Abbott requires a list of registrants in advance,=20

and we need to plan for lunch. See you on October
29 {/fontfamily} {fontfamily} {param} Times {/param} th!



{/fontfamily}






From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Wed, 9 Oct 1996 22:07:08 -0500
Subject: Scanning Electron Microscopy SEMINAR - Oct 25th

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ASM CHICAGO ELECTRONIC MATERIALS CHAPTER

In Collaboration With

The CHICAGO and CHICAGO WESTERN CHAPTERS Of ASM INTERNATIONAL

The MIDWEST MICROSCOPY andMICROANALYSIS SOClETY
and
The MATERIALS SCIENCE DIVISION of ARGONNE NATIONAL LABORATORY

presents

"Scanning Electron Microscopy Today"
An All-Day Educational Seminar

Friday, October 25, 1996
Advanced Photon Source Conference Center Auditorium
Argonne National Laboratory

8:30 am-4:30 pm

The lecturers for this seminar will review in some depth the basic
principles of SEM, including the basic concepts of SEM operation and
specimen preparation, imaging mechanisms in various operational modes,
field emission SEM (FESEM), low voltage SEM (LVSEM) and environmental SEM
(ESEM). The lecturers include Professor David Joy, University of Tennessee
Knoxville (sponsored by Nissei Sangyo America/ Hitachi Scientific
Instruments) and Vernon Robertson, JEOL Applications Laboratory (sponsored
by JEOL USA, Inc.). Whether you are a beginner or a long-time user of
scanning electron microscopy, you will find the lecturers and their lecture
material interesting, informative and uptodate. Extensive discussion is
encouraged. Registration fee is $20 per person (students, $10), which
includes a buffet lunch and refreshments during morning and afternoon
breaks and should be paid in advance. To register, complete and mail the
form below, along with a check or money order for registration. If you are
not a US citizen (even if you hold a Green Card), you must provide city and
date of birth in order for us to arrange for your entry to the Laboratory
site. Please register early and save everyone considerable hassle. The day
of the Seminar, come directly to North Gate where the security guard will
check your name on a list of attendees. Bring photo ID (e.g., drivers
license).

................................................................................
........................




SEM Seminar Registration (Oct. 25, 1996)

Name:____________________________
Organization:______________________
Mailing Address:____________________
FAX:______________________________
Phone_____________________________
E-Mail____________________________

..........................................................................

Today's Date________________________

Membership: [ASM] [MMMSl [ ]
Citizensh ip:________________________
City of Birth:________________________
Date of Birth:________________________

Registration Fee $20 (students: $10) enciosed.
Mail to: C. W. Allen, MSD-212/E211, Argonne
National Laboratory, Argonne, IL 60439-4838

(Questions or Information: Phone: 630-252-4157 or Email:
allen-at-aaem.amc.anl.gov)






From: houtsmuller-at-pa1.fgg.eur.nl (Adriaan Houtsmuller)
Date: Thu, 10 Oct 1996 10:00:35 +0100
Subject: Re: www

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X-Sender: houtsmul-at-hp750.fgg.eur.nl
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At 07:08 PM 10/4/96 BST-1, Brian Ford wrote:
}
}
} We have a new web page. You will find it on:
}
} www.sciences/demon.co.uk
}
} We have e-mail on demon, too:
}
} brian-at-sciences.demon.co.uk
}
} Let me know what you think of the web page - it's only provisional, I
} shall not really announce it 'til Christmas.
}
} Best wishes,
} Brian J Ford
}

Maybe I'm wrong, but I consider the announcement of a webpage on such a
large mailing list as an official announcement. Moreover, if it concerns a
PROVISIONAL webpage (don't we all have one) I think it is something like
announcing the submission of a paper.

Possibly it's better to announce these type of things on a smaller
platform, like colleagues and friends.

If no one agrees with me I'm sorry to have bothered you.

A.B. Houtsmuller

__ __
__ /_/ /_/
/_/
____ ___
/ / / |
/ / / / *Dept. of Pathology,
/ --- / *Erasmus University,
/ / *P.O. BOX 1738,
/__ ** | *3000 DR Rotterdam,
/ | *The Netherlands,
__/ | *Phone +31 10 4087499
\________ / *Fax +31 10 4366660
| \
/ |
----






From: Analytical Imaging Facility :      aif-at-telico.bioc.aecom.yu.edu
Date: Thu, 10 Oct 1996 10:40:54 -0400 (EDT)
Subject: heat load question

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We are undergoing renovation of our shared microscopy facility and need
specific heat load information for each piece of equipment in order to
convince the engineers of the need for dedicated air cooling/ventilation.
Does anybody know the following or a reference for the following:
The heat load for:
100W mercury lamp
100W halogen lamp
Nikon incubator boxes NP-2
Olympus incubator boxes
Sony 17" monitors (computer)
Sony 14" monitors
computers
VCRs
anything else that we may have overlooked (each person is calculated as 75W)

Thanks-
Michael Cammer






From: Franklin Bailey :      jfb-at-novell.uidaho.edu
Date: Thu, 10 Oct 1996 08:43:41 PST8PDT
Subject: unsubscribe

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Please unsubscribe




From: Mary A. Molter :      molter-at-post.its.mcw.edu
Date: Thu, 10 Oct 1996 11:04:44 -0500 (CDT)
Subject: unsubscribe

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unsubscribe Mary Molter





From: Joseph P. Neilly 847-938-5024 :      NEILLY.JOSEPH-at-igate.pprd.abbott.com
Date: Thu, 10 Oct 1996 08:44:00 -0500 (CDT)
Subject: MMMS Meeting:Microscopy in Healthcare - Oct. 29

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Mr-Received: by mta RANDD; Relayed; Thu, 10 Oct 1996 09:31:43 -0500
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Midwest Microscopy and Microanalysis Society Meeting

Microscopy: A Problem - Solving Tool in the Healthcare Industry

Tuesday, October 29, 1996
Abbott Laboratories
Abbott Park, Illinois



12:00 - 1:00 Lunch (provided by Abbott Laboratories)

1:00 - 1:15 Welcome

1:15 - 2:00 Pharmaceutical Sleuthing - Applications of Light Microscopy
Scott Aldrich, Trace Substance Analysis, Pharmacia & Upjohn, Inc.;
Kalamazoo MI

2:00 - 2:45 Imaging Applications in the Pharmaceutical Industry: Not
Just Pretty Pictures
Michail Esterman, Technology Core Research, Lilly Research Laboratories,
Indianapolis IN

2:45- 3:15 Break

3:15 - 4:00 Electron Microscopy in Pathology: Under the Regulatory
Microscope
Sandy L. White and Jeffrey W. Horn, Toxicology Research
Laboratories, Lilly Research Laboratories, Greenfield IN

4:00 - 4:45 Microscopy in Medical Product Development
Jim DiOrio, Medical Materials Technology Center, Baxter Healthcare
Corporation, Round Lake IL

4:45 Tour of the Department of Microscopy and Microanalysis

To register, please contact Jerry Gagne by October 11 phone (847-938-
5023), FAX (847-938-5027) or E-mail (gerard.d.gagne-at-abbott.com)

Please help us out by registering ahead of time! The main security gate
at Abbott requires a list of registrants in advance, and we need to plan
for lunch. See you on October 29th!


Additional information will be posted on the MMMS WWW Site:
http://www.msa.microscopy.com/MSALAS/MMMS/MMMSHomePage.html





From: Richard Zeyen - richz-at-puccini.crl.umn.edu
Date: Thu, 10 Oct 1996
Subject: unsubscribe

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Please unsubscribe


Richard J. Zeyen
Work: Department of Plant Pathology
University of Minnesota
St. Paul, MN 55108

Office Phone + Voice Mail
(612) 625-4754
E-Mail
richz-at-puccini.crl.umn.edu





From: mgeorge-at-matsci.uah.edu (Michael A. George)
Date: Fri, 11 Oct 1996 02:58:05 +0500
Subject: unsubscribe

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Message-Id: {m0vBQca-000UK4C-at-biolo.bg.fcen.uba.ar}

Please unsubscribe mgeorge-at-matsci.uah.edu






From: kjones-at-es.co.nz (kathryn jones)
Date: Fri, 11 Oct 1996 10:54:28 +1200
Subject: Poly-L-lysine

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Hi Robert

} 1. What is the shelf life of an aqueous solution of poly-L-lysine?
I would think only a few days. I made a concentrated solution up under
sterile conditions, aliquoted it and froze it at -20. It lasts for 6
months if frozen.
} 3. When making lysine coated cover slips, the procedure (as I understand
} it) is to cover a cover slip with an aqueous solution of poly-L for one
} hour then dab dry.
Iwas using the coverslips for culturing work. I would coat the coverslips
for 10 minutes, remove the PLL, rinse the coverslips 2X with deionized
water and then let them dry.
} Can that drop of solution be transferred to a fresh
} cover slip or has its supply of lysine been exhausted?
Partially exhasuted and you may want the same concentration of PLL to be on
each coverslip.

Kathryn

Kathryn R. Jones BSc (Hons)
k.jones-at-es.co.nz






From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 10 Oct 1996 16:37:56 -0700
Subject: Thanks to all re Cu in trichomes

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Please let me take a moment to thank everyone who offered suggestions on
how to solve our problem of localizing Cu in trichomes. We got some great
ideas and will try to move forward on the project.

Also, let me express my gratitude to Nestor and each of you who read and
contribute to this list. For me, this list represents a tremendous source
of knowledge and help when I need advice. It provides a helpful reality
check whenever I wonder if what I want to do is not quite thought out or
needs some sage advice from those with more experience than I.

Thanks again,

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
FAX (408) 429-0146
jmkrupp-at-cats.ucsc.edu






From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 10 Oct 1996 16:37:56 -0700
Subject: Thanks to all re Cu in trichomes

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Message-Id: {199610102333.QAA14781-at-cats.ucsc.edu}
Mime-Version: 1.0
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Please let me take a moment to thank everyone who offered suggestions on
how to solve our problem of localizing Cu in trichomes. We got some great
ideas and will try to move forward on the project.

Also, let me express my gratitude to Nestor and each of you who read and
contribute to this list. For me, this list represents a tremendous source
of knowledge and help when I need advice. It provides a helpful reality
check whenever I wonder if what I want to do is not quite thought out or
needs some sage advice from those with more experience than I.

Thanks again,

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
FAX (408) 429-0146
jmkrupp-at-cats.ucsc.edu






From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Fri, 11 Oct 1996 10:06:10 +1000 (EST)
Subject: Re: heat load question

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} We are undergoing renovation of our shared microscopy facility and need
} specific heat load information for each piece of equipment in order to
} convince the engineers of the need for dedicated air cooling/ventilation.
} Does anybody know the following or a reference for the following:
} The heat load for:
} 100W mercury lamp
} 100W halogen lamp
} Nikon incubator boxes NP-2
} Olympus incubator boxes
} Sony 17" monitors (computer)
} Sony 14" monitors
} computers
} VCRs
} anything else that we may have overlooked (each person is calculated as 75W)
}
} Thanks-
} Michael Cammer
You dont' go far wrong (and err on the safe side anyway) if you just take
the wattage of the equipment as representing the heat load. In Australia we
express heat load in watts anyway. If its different in the US a competent
Air-conditioning engineer can convert for you.

Mel Dickson
}
}
}





From: hartz stephanie ( phd mocb) :      shartz1-at-gl.umbc.edu
Date: Thu, 10 Oct 1996 15:46:19 -0400 (EDT)
Subject: Confocal question doing double label colocalization

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X-Authentication-Warning: umbc8.umbc.edu: prutle1 owned process doing -bs

This waas sent to me byu a colleague and hopefully someone can help her.
I have little experience doing double labeling.
Thanks.

Peace through Christ,

Phil Rutledge, Director e-mail: prutle1-at-gl.umbc.edu
Center for Microscopy voice: (410) 455-3582
UMBC Dept. of Biology fax: (410) 455-3875
Catonsville, MD 21228
////
/ /
/ /
/////// ///////
/ /
/////// ///////
/ /
/ /
/ /
/ /
/ /
/ /
/////

---------- Forwarded message ----------
prutle1-at-umbc.edu, Tom Roth {roth-at-umbc.edu}


I have been trying to use the confocal microscope to determine when
the chicken protein LEP100 arrives in the lysosomes of transiently
expressing Drosophila tissue culture cells. I have not had much success,
and would greatly appreciate any help or advice you could give me.

} From the experiment I usually see about 1-5% of the cells expressing
LEP100. The amount of protein expressed can vary from cell to cell,
depending on the copy number. To determine whether or not LEP100 reaches
the lysosomes, I prelabel the lysosomes with Fluorescence Dextran. I then
localize LEP100 by indirect immunofluorescence using
RITC-conjugated secondary antibody. I see lysosomal labeling in the
green channel and LEP100 staining in the red channel, but there
intensities are different. There appears to be
colocalization using the naked eye. THe problem comes in when I try to
sequentially scan each image and superimpose the images. I don't see
colocalization. That is I don't see yellow areas where the red and green
areas overlap.

The procedure that I am using on the confocal microscope (LEICA TCS 4D) is
as follows: I
first select optical fiters for channel 1 (FITC, with excitation of
488nm). Next, I adjust the voltage, offset, and pinhole for optimal image
quality in channel 1. This typically results in a voltage of 550, an
offset of -2, and a pinhole of 100 (for the 100X objective). The laser
power is about 9. I next define the top and bottom of the Z series, set
the memory bar to the number of sections to be taken, and then scan the
image doing an average of 4 scans per frame.
Then I go back and select optical filters for channel 2 (RITC,
with excitation of 568nm). I then adjust the voltage and offset for
optimal image quality when in channel 2. This typically results in a
voltage of 920 and an offset of -1. I do not change the dimensions for
the Z series, laser power, or pinhole size. After scanning the same image
series in channel 2, I go to the TCD monitor to display the images. TO get
the best resolution, I just look at one section of the image series at a
time. I go to the Macrohandling window and click on gallery all for 2
channels. What I see on the TCD display monitor is the image of one
section in channel 1 (lysosomes), and the image of the same section
scanned in channel 2 (LEP100) superimposed on top of the channel 1
image. I see green areas and red areas which appear to overlap, but the
color is not yellow. Do you know what is going on? Please help me if you
can. Thank you.

Sincerely,

Stephanie Hartz
X2246





From: kuo-at-jrcat.or.jp (LI-HSIN KUO)
Date: Fri, 11 Oct 1996 10:08:16 -0600
Subject: request for the information

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Message-Id: {199610110059.JAA02043-at-mserver.jrcat.or.jp}

Dear editor,

This is a request from L. H. Kuo. I am a electron microscopist. I would
appreciate
very much if you can transfer the information related to electron microscpy
from your internet connection.

Thank you very much.

Best regards,

Li-hsin Kuo.

*******************************************************
Joint Research Center for Atom Technology (JRCAT),
c/o National Institute for Advanced Interdisciplinary Research (NAIR),
1-1-4 Higashi, Tsukuba, Ibaraki 305, Japan.
Tel: 81-298-54-2739 Fax: 81-298-54-2777
*******************************************************





From: Sara Miller :      saram-at-acpub.duke.edu
Date: Thu, 10 Oct 1996 19:19:31 -0400 (EDT)
Subject: Re: www

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On Thu, 10 Oct 1996, Adriaan Houtsmuller wrote:

} Date: Thu, 10 Oct 1996 10:00:35 +0100
} From: Adriaan Houtsmuller {houtsmuller-at-pa1.fgg.eur.nl}
} To: Microscopy-at-aaem.amc.anl.gov
} Subject: Re: www
}
} At 07:08 PM 10/4/96 BST-1, Brian Ford wrote:
} }
} }
} } We have a new web page. You will find it on:
} }
} } www.sciences/demon.co.uk
} }
} } We have e-mail on demon, too:
} }
} } brian-at-sciences.demon.co.uk
} }
} } Let me know what you think of the web page - it's only provisional, I
} } shall not really announce it 'til Christmas.
} }
} } Best wishes,
} } Brian J Ford
} }
}
} Maybe I'm wrong, but I consider the announcement of a webpage on such a
} large mailing list as an official announcement. Moreover, if it concerns a
} PROVISIONAL webpage (don't we all have one) I think it is something like
} announcing the submission of a paper.
}
} Possibly it's better to announce these type of things on a smaller
} platform, like colleagues and friends.
}
} If no one agrees with me I'm sorry to have bothered you.
}
} A.B. Houtsmuller
}
} __ __
} __ /_/ /_/
} /_/
} ____ ___
} / / / |
} / / / / *Dept. of Pathology,
} / --- / *Erasmus University,
} / / *P.O. BOX 1738,
} /__ ** | *3000 DR Rotterdam,
} / | *The Netherlands,
} __/ | *Phone +31 10 4087499
} \________ / *Fax +31 10 4366660
} | \
} / |
} ----
}

I agree. At least you could tell us in the subject heading what it's
about. I, for one, don't have time to look it up just to see if I'm
interested.}

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: Edward J. Huff :      huffe-at-carbon.chem.nyu.edu
Date: Thu, 10 Oct 1996 23:12:06 -0400
Subject: Re: www

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(houtsmuller-at-pa1.fgg.eur.nl)

} We have a new web page. You will find it on:
} http://www.sciences.demon.co.uk [corrected]
} Brian J Ford

} Maybe I'm wrong, but I consider the announcement of a webpage on such a
} large mailing list as an official announcement....
} A.B. Houtsmuller

I suspect that sending the message to the microscopy list was
unintended, considering the long list of addresses on the original
note. I would have ignored this except that I took the time to find
out that the '/' should be a '.'. My apologies to those who can't
rapidly scan over junk mail, but there are better mail readers
available...

Anyway, readers of the list should be careful about putting
the list address into mail aliases to which they might later send
something that doesn't belong.




From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Fri, 11 Oct 1996 07:18:56 -0500
Subject: Message from Nestor to Everyone

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G'day Subscribers...


Just a short note to let you all know that I will be
out of the country from Oct 12-20. This guarenttee's,
of course, that SOMEONE SOMEWHERE will attempt
SOMETHING that will upset the Listserver and send
it into chaos (why should this trip be different
than any others, right?). Unfortunately, my connection to the
Net will not be very good so be prepared for a possible
hickup that may not be immediately noticed by yours truely.

If your not sure of something today is the day to ask
the question as I'll be leaving tomorrow, Saturday Oct. 12.

--------------

Just as a reminder to UNSUBSCRIBE

send a message to: LISTSERVER-at-MSA.MICROSCOPY.COM

the message type UNSUBSCRIBE MICROSCOPY "USERID-at-YOUREMAILADDRESS".

If you send these messages to MICROSCOPY instead of LISTSERVER, while
I am gone I can almost guarentee you will NOT be unsubscribed since I
will not be monitoring the postings and will NOT catch your errors.

If you think you will be unsubscribing during the next week, why not
do it today to save possible hickups to the system while I'm gone.

---------------


Nestor
Your Friendly Neighborhood SysOp

For those of you that are curious I will be at the Microscopy & Materials
Conference
(MicroMat - 96) in Rio de Janerio.






From: evansnd-at-ornl.gov (Neal D. Evans)
Date: Fri, 11 Oct 1996 09:06:35 -0400
Subject: heat loads from laboratory equipment

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Someone asked for estimated heat loads from laboratory equipment. An easy
and reasonable approximation is to assume all power consumed is converted to
heat. So that computer (w/200 watt power supply) and monitor (200 watts)
will dump up to 400 watts into a room; don't adjust this estmate to reflect
that portion of time the system is off or asleep because you want the HVAC
to be able to handle the maximum loads.







From: lizard-at-okway.okstate.edu (Ginger Baker)
Date: Fri, 11 Oct 1996 08:51:32 -0700
Subject: poly-l-lysine

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Bob,

Here is the procedure I use:

1. Clean coverslips (first rinse with methanol, then water)
2. 1ug/ml polylysine solution (high molecular weight)
3. fixed cells
5. standard aldehyde solution

Spread a drop of the polylysine solution on a clean coverslip.
Spread the drop by drawing the edge of one coverslip over the surface
of the other. Allow the coverslip to dry for 1 min. Rinse with
double distilled water. Place a drop of the sample that is in water
or buffer on the reactive surface. Wait for the sample to settle down
on the surface, usually between 5-20 min. Place coverslips in
aldehyde solution for 15 min. Proceed as normal for dehydration and
drying.

Coverslips must be clean or polylysine won't stick. Unfixed cells
will adhere but they tend to flatten out on the surface. A light
fixation before putting them on the coverslips helps prevent this
occurrence. The polylysine I use has been in the refrigerator for
several months. The container I used previously lasted almost two
years. You want a good growth of cells. I make up my coverslips fresh.



Ginger R. Baker
EM Lab
Dept. of Anatomy, Pathology, and Pharmacology
250 Vet Med
OSU
Stillwater, OK 74078
(405) 744-6765
FAX: (405) 744-5275
Email: lizard-at-okway.okstate.edu




From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Fri, 4 Oct 1996 12:39:00 +1000 (EST)
Subject: Re: SEM atlas

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This message is automatically generated.

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Original message follows
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Good books with SEM pictures

Scanning Nature
D. Claugher
British Museum
ISBN 0521 25705 0 Hardback
GBPound 5.5

Tissues and Organs
R.G. Kessel & R.H. Kardon
W.H. Freeman S.F.
ISBN 0-7167-0091-3
}
}





From: Glenn Poirier :      glennp-at-eps.mcgill.ca
Date: Fri, 11 Oct 1996 13:58:53 -0400 (EDT)
Subject: Re: Carbon Contamination rates

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Thanks to all who replied regarding my question on carbon contamination
rates. It is really nice to be able to get the attention of so much
expertise so easily. If anyone is interested I can send them a copy of
all the replies I received, you can e-mail me at the address below.
I plan to wait until my cold-finger gets installed (hopefully
next week) and to try and find a place on campus to get the samples
sputter cleaned (Getting them back to the lab will be another story :-)).

Thanks again for all the help

Glenn




From: Richard Thrift :      Richard_Thrift-at-depotech.com
Date: Fri, 11 Oct 1996 13:28:12 -0800
Subject: Fluorescent labels for triglyceride vs phospholipid?

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Message-Id: {s25e4b67.042-at-depotech.com}
X-Mailer: Novell GroupWise 4.1

Hi
I'm studying structure of emulsions of phospholipids, cholesterol, and
triglycerides by fluorescence microscopy. I would appreciate hearing
suggestions or comments about probes to use that might specifically
label triglyceride droplets but not phospholipid membranes. I can work
with a label added either before or after the emulsion is made.

For example I can specifically label phospholipid membranes using
phospholipids with a fluorescent headgroup such as Bodipy-FL. I have
used Nile Red (added either during or after manufacture of the emulsion)
to simultaneously stain neutral lipids, but it also stains the membranes to
some extent. It is also hard to work with because it photobleaches so
quickly. Has anyone found useful alternatives to Nile Red for
more-or-less specifically staining neutral lipid? Have you found a good
additive that can minimize photobleaching of Nile Red?

Does DiI stain triglyceride droplets well, and would any related
compound work better?
Is NBD-cholesterol useful for imaging? (My impression is that it might
photobleach rapidly.)

Thanks very much
Richard Thrift
Depotech Corp.
Richard_Thrift-at-Depotech.com





From: Richard Thrift :      Richard_Thrift-at-depotech.com
Date: Fri, 11 Oct 1996 14:30:41 -0800
Subject: Questions on DiI transport, flip-flopping etc

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Message-Id: {s25e5a11.060-at-depotech.com}
X-Mailer: Novell GroupWise 4.1

Hi again
I'm looking for a fluorescent probe that can demonstrate whether
membranes are continuous or not. If I add the probe to the outer
membrane I want it able to label internal membranes IF AND ONLY IF
they are continuous with the external membrane. (In a dead or
ATP-depleted cell, for instance. I'm actually studying liposomes.) It
should be able to flip-flop and label the opposite leaflet of the bilayer to
which it is exposed. (Actually, a probe that is unable to flip-flop would
be useful, too, though not as useful.) It should spread only by diffusion
in the "plane" of the membrane, once the carrier solvent dissipates.

Can you tell me whether DiI (and related dyes) flip-flop rapidly across
membranes to label both sides of a bilayer in absence of ATP)? I've
heard DiI labels all internal cell membranes; does it diffuse through
cytoplasm or is it constrained to stay in the membrane? Can it jump
from one membrane to another that is very closely apposed but not
continuous? Is it a substrate for lipid transfer/exchange proteins,
including albumin? Can you suggest any good references on properties
of these dyes, or regarding their use for this particular purpose?

Pardon my ignorance; any suggestions would be greatly appreciated!
THANKS very much !
Richard Thrift
Depotech Corp.
Richard_Thrift-at-Depotech.com
(619) 625-2424





From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: Fri, 11 Oct 1996 16:01:02 -0700
Subject: Prepared LM Slides

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Mime-Version: 1.0

Hi All;

A friend of mine would like to obtain some prepared LM slides for teaching
purposes (such as blood cells, onion skin, etc.). Any suggestions as to a
relatively inexpensive source? TIA

-Bob
**********************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA 91711
ph: (909)399-1311
email: Bob_Citron-at-cc.chiron.com
**********************************




From: Laurent Heliot :      Laurent.Heliot-at-imag.fr
Date: Sat, 12 Oct 1996 11:19:40 +0000
Subject: STEM-Realignement for Tomography

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Hi,
I work in biology with a STEM. I make tomographic reconstruction with a
serial tiltes views, but I have a problem about realignement of projections
because during the acquisition the rotation axis is shifted (5-10 pixels in X
direction).
Do you have a suggestion ????
Think you for your help.

Laurent Heliot

--


----------------------------**********-----------------------------------
Laurent HELIOT LABORATOIRES DYOGEN/INFODIS
(Post-doctorant) Institut Albert Bonniot,
Mots Clefs : GRENOBLE
Nucleolus, Nucleus, chromosome, Domaine de la MERCI
AgNOR, Tomography, STEM, reconstruction 3D 38700 La TRONCHE
----------------------------------------------------------------------------




From: Scott D. Ireland :      sireland-at-frontiernet.net
Date: Sat, 12 Oct 1996 06:38:04 -0400
Subject: Re: Prepared LM Slides

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Message-Id: {199610121040.GAA40512-at-mail.frontiernet.net}
{microscopy-at-sparc5.microscopy.com}

How about Edmund Scientific in NJ - call 609-573-6250 for a catalog.

Scott D. Ireland
Media Cybernetics, LP
" The Imaging Experts "
scott-at-mediacy.com
(716) 473-0222 Tel
(716) 473-8048 Fax

----------
} From: Bob Citron {Bob_Citron-at-cc.chiron.com}
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: Prepared LM Slides
} Date: Friday, October 11, 1996 7:01 PM
}
} Hi All;
}
} A friend of mine would like to obtain some prepared LM slides for
teaching
} purposes (such as blood cells, onion skin, etc.). Any suggestions as to
a
} relatively inexpensive source? TIA
}
} -Bob
} **********************************
} Bob Citron
} Chiron Vision
} 555 W. Arrow Hwy
} Claremont, CA 91711
} ph: (909)399-1311
} email: Bob_Citron-at-cc.chiron.com
} **********************************




From: Caroline Schooley :      schooley-at-mcn.org
Date: Sat, 12 Oct 1996 09:11:25 -0700
Subject: Re: Prepared LM Slides

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Mime-Version: 1.0
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} Hi All;
}
} A friend of mine would like to obtain some prepared LM slides for teaching
} purposes (such as blood cells, onion skin, etc.). Any suggestions as to a
} relatively inexpensive source? TIA

Basics like that are carried by Carolina Biological Supply:800-334-5551.
If this is for precollege education, consider the "do-it-yourself" approach
rather than prepared slides - see the ProjectMICRO website. I'll be happy
to suggest specific books.

Caroline Schooley
Educational Outreach Coordinator, Microscopy Society of America
Box 117 (45301 Caspar Point Road)
Caspar, CA 95420
Phone/FAX: (707)964-9460
Email: schooley-at-mcn.org
Web: http://www.MSA.microscopy.com/ProjectMICRO/Books.html






From: geoffa-at-amsg.austmus.gov.au (GeoffA)
Date: Mon, 14 Oct 1996 10:18:50 +1100
Subject: Microscopists in Singapore?

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Mime-Version: 1.0

If there are any Singaporean microscopists on the List who are willing to
have a chat about work and general life in the region, would you please
contact me direct on the email address below.

Thanks,

::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
Geoff Avern
Manager
Microscopy Laboratories
Australian Museum Email: geoffa-at-amsg.austmus.gov.au
6 College St Ph: (61)(2) 9320 6198
Sydney, Australia. 2000 Fax: (61)(2) 9320 6059
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::








From: Philip H. IMAMURA :      phimamur-at-uci.edu
Date: Sun, 13 Oct 1996 21:33:38 -0700 (PDT)
Subject: Litserve

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I am writing to find out how to subscribe to the microscopy litserve.
Any assistance will be apppreciated.

thanks,

Phili Imamura





From: rh208-at-cus.cam.ac.uk (Ray Hicks)
Date: Mon, 14 Oct 1996 12:26:00 +0100
Subject: Re: Questions on DiI transport, flip-flopping etc

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Hi Richard,

A lateral response to your question: If your membranes include
phosphatydilserine, you could try labelling them with Annexin, which is a
short peptide that is specific for PS. It is available conjugated to
fluorescein or biotin. It's used to study apoptosis, where PS, which is
normally restricted to the inner lamella, flips over to the outer, allowing
Annexin V to bind and label the cell.

Note that Annexin V isn't actually incorporated into the membrane, but
binds strongly to components whose migration it would reveal. It only
binds in the presence of fairly high calcium concentrations, and can be
removed from membranes by washing in a calcium-free buffer.


Ray


VAt 2:30 pm 11/10/96, Richard Thrift wrote:
} Hi again
} I'm looking for a fluorescent probe that can demonstrate whether
} membranes are continuous or not. If I add the probe to the outer
} membrane I want it able to label internal membranes IF AND ONLY IF
} they are continuous with the external membrane. (In a dead or
} ATP-depleted cell, for instance. I'm actually studying liposomes.) It
} should be able to flip-flop and label the opposite leaflet of the bilayer to
} which it is exposed. (Actually, a probe that is unable to flip-flop would
} be useful, too, though not as useful.) It should spread only by diffusion
} in the "plane" of the membrane, once the carrier solvent dissipates.
}
} Can you tell me whether DiI (and related dyes) flip-flop rapidly across
} membranes to label both sides of a bilayer in absence of ATP)? I've
} heard DiI labels all internal cell membranes; does it diffuse through
} cytoplasm or is it constrained to stay in the membrane? Can it jump
} from one membrane to another that is very closely apposed but not
} continuous? Is it a substrate for lipid transfer/exchange proteins,
} including albumin? Can you suggest any good references on properties
} of these dyes, or regarding their use for this particular purpose?
}
} Pardon my ignorance; any suggestions would be greatly appreciated!
} THANKS very much !
} Richard Thrift
} Depotech Corp.
} Richard_Thrift-at-Depotech.com
} (619) 625-2424






From: agoldsch-at-tamarugo.cec.uchile.cl (GOLDSCHMIDT DE LA MATTA ALFONSO)
Date: Mon, 14 Oct 1996 11:56:45 +0400
Subject: i need contact with MSABusinessOffice@MSA.Microscopy.Com

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Dear friend :
The mail addres of Microscopy-at-MSA.Microscopy.Com may I have
not writh , because the E-mail retuurn to me


thanks in advance


A.Goldschmidt




From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: Mon, 14 Oct 1996 07:50:05 -0700
Subject: Micro Slides - Thanks

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Just wanted to thank everyone who replied so quickly to my question about
prepared microscope slides. The most highly recommended sources included
Triarch (Ripon, WI) and Carolina Biological Supply (800) 334-5551.

-Bob
********************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA 91711
(909)399-1311
email: Bob_Citron-at-cc.chiron.com
********************************




From: JOSEPH COHEN :      JDCOHEN-at-stoel.com
Date: Mon, 14 Oct 1996 08:32:00 -0700
Subject: Re: Prepared LM Slides -Reply

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Another catalog source is NASCO. Their scientific catalog has many more
slides of basic biological specimens than Edmund's catalog.
I took the following information is from NASCO's web site
http://www.nascofa.com/
(You can order a catalog via the web site.)
______
Phone:
Call Toll Free - U.S.A. & Canada 1-800-558-9595
If ordering from outside the U.S.A., call 1-414-563-2446

NASCO ? FORT ATKINSON
901 Janesville Avenue
P.O. Box 901
Fort Atkinson, WI 53538-0901
E-mail: info-at-nascofa.com
Fax: 414-563-8296

NASCO ? MODESTO
4825 Stoddard Road
P.O. Box 3837
Modesto, California 95352-3837
E-mail: modesto-at-nascofa.com
Fax: 209-545-1669

PHONE ORDER HOURS:
Weekdays 7:00 AM - 6:00 PM
Saturday 8:00 AM - 12:00 Noon
Fort Atkinson, WI (CT) Modesto, CA (PT)

} } } Scott D. Ireland {sireland-at-frontiernet.net} 10/12/96 03:38
} } }
How about Edmund Scientific in NJ - call 609-573-6250 for a catalog.

Scott D. Ireland
Media Cybernetics, LP
" The Imaging Experts " scott-at-mediacy.com
(716) 473-0222 Tel
(716) 473-8048 Fax

----------
} From: Bob Citron {Bob_Citron-at-cc.chiron.com}
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: Prepared LM Slides
} Date: Friday, October 11, 1996 7:01 PM
} } Hi All;
} } A friend of mine would like to obtain some prepared LM slides for
teaching } purposes (such as blood cells, onion skin, etc.). Any
suggestions as to a } relatively inexpensive source? TIA
} } -Bob
} **********************************
} Bob Citron
} Chiron Vision
} 555 W. Arrow Hwy
} Claremont, CA 91711
} ph: (909)399-1311
} email: Bob_Citron-at-cc.chiron.com
} **********************************






From: OSRAMWG-at-aol.com
Date: Mon, 14 Oct 1996 18:30:58 -0400
Subject: HBO 50 W/AC: lamp for microscopes: start up time

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STATEMENT NR. 3

issued by OSRAM GmbH, Photo-Optics Marketing, Berlin
Fax: (Germany)-30 -3386 -2773, e-mail: osramwg-at-aol.com

re: HBO 50 W/AC, lamp for microscopes:start up..

Dear subscribers,

this is now the third statement, related to "start-up time".

Some of you complained about the startup time in HBO 50 W/AC lamps.

Beginning of September, we have started delivery of a number of slightly
modified lamps.
The obvious difference is, that the lamps have a reflector on both sides of
the electrodes (standard lamp has only one reflector). These lamps are 100%
compatible to the standard lamps, however we found a reduced start-up time.
It will be important for us to get your feedback.
If you find your next lamp with reflectors on both sides we would like to ask
you to give us some kind of feedback. This should help you and us as well by
an increased performance of the lamps in the future.

Thank you very much for your support.

Statement NR. 4 will target "low output power from the lamp"




From: r.g.white-at-sci.monash.edu.au (Rosemary White)
Date: Tue, 15 Oct 1996 10:15:18 +1200
Subject: job opening

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I would be grateful if you could let postdoctoral people in your department
know that there was an advertisement for a Marine Protistan Biologist in
the Antarctic Division in the WEEKEND AUSTRALIAN on 12 October.

Cheers

Harvey

*********************************************************************
Harvey J. Marchant
Program Leader, Biology
Australian Antarctic Division
Channel Highway, Kingston, Tasmania 7050
Australia

phone: (03) 62 323 323 international + 61 3 62 323 323
fax: (03) 62 323 351 international + 61 3 62 323 351
mobile: 0419 390 492
*********************************************************************






From: OSRAMWG-at-aol.com
Date: Mon, 14 Oct 1996 18:30:54 -0400
Subject: HBO 50 W/AC, lamp for microscopes, no or difficult ignition

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issued by OSRAM GmbH, Photo-Optics Marketing, Berlin
Fax: (Germany)-30 -3386 -2773, e-mail: osramwg-at-aol.com

re: HBO 50 W/AC, lamp for microscopes, no or difficult ignition

Dear subscribers,

this is not a commercial advertisement but (hopefully) valuable information
to all
microscopists, who recently faced problems with OSRAM lamps in their
microscopes.
The first statement is related to " no starters or difficult ignition".
End of 1995, we have replaced a machine in production. This new machine had
at the
beginning a broader tolerance regarding fill pressure and therefore a small
number of lamps have been produced with a wrong fill pressure, causing
ignition problems. However it took several month until we heard about these
problems and until the failure was located. Corrective actions have been
taken and the problem should not exist any longer.
However, dependent on the stock situation of the different microscope
manufacturers, service organizations and lamp dealers, there are probably
still a certain number of these lamps in the field.
If you do have any problem with ignition AND your lamp shows the following
serial numbers: xxxx qP2....xxxxqP6 or xxxxqk1.... qk4, please report to
your dealer or to us directly. Your lamps will be replaced free of charge.


Statement NR. 2 will follow soon and contains information about reported lamp
explosions




From: OSRAMWG-at-aol.com
Date: Mon, 14 Oct 1996 18:31:00 -0400
Subject: HBO 50 W/AC, HBO 100 W/2 and HBO 103W/2: low output power

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STATEMENT NR. 4

issued by OSRAM GmbH, Photo-Optics Marketing, Berlin
Fax: (Germany)-30 -3386 -2773, e-mail: osramwg-at-aol.com

re: HBO 50 W/AC, HBO 100 W/2 and HBO 103W/2, lamp for microscopes: low output
power on Nikon microscopes

Dear subscribers,

this is now statement NR. 4, related to low light output.

Two reasons in general might cause low output:
- blackening of the bulb (aging)
- low current from the psu

Blackening is dependent on the voltage, current (also power supply!), type of
current (DC vs., AC) electrode material, gas purity etc... etc. With HBO 50
W/AC it is an aging process.

Low output from the microscope with HBO 100 W/2 or HBO 103 W/2 it is mostly
related to the characteristics of the power supply. An HBO lamp has a burning
voltage depending on the lamp itself and draws as much current as it can get.
The power supply however powers the lamp depending on the lamp voltage. For
Nikon microscopes for example, the power to the lamp will be between 60 and
100 W depending on the lamp voltage (which increases as a function of
lifetime- if you are interested in this graph- please send an e-mail, we will
get it to you immediately).
Other microscope manufacturers have decided for constant power mode. In this
case, the lamp demands increasing current over lifetime and you will get a
more or less constant output until the end of lifetime.

If you do have any problem with Nikon equipment regarding low output power,
we recommend to contact your Nikon dealer. We were told today from NIKON
Yokohama, that there is a problem with their power supplies, however up to
now, we do not have more detailed information.

The last statement NR. 5 will summarize faq's from the microscopy list
subscribers during the last weeks




From: OSRAMWG-at-aol.com
Date: Mon, 14 Oct 1996 18:30:57 -0400
Subject: HBO 50 W/AC, HBO 100 W/2 and HBO 103 W/2, : lamp explosions

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STATEMENT NR. 2

issued by OSRAM GmbH, Photo-Optics Marketing, Berlin
Fax: (Germany)-30 -3386 -2773, e-mail: osramwg-at-aol.com

re: HBO 50 W/AC, HBO 100 W/2 and HBO 103 W/2, lamp for microscopes: lamp
explosions

Dear subscribers,

this is now the second statement, related to "lamp explosions"

A very small number of you (6 people) reported, that they have been faced
with lamp explosions in their microscopes.
We have investigated this situation here in the company, however within the
last years (100.000 produced lamps) no explosions have been reported. The
questions was: When does a lamp explode (beside operation above the rated
lifetime)?-either due to a defect in the bulb (microcrack etc.) or due to
overheating in the lamp (by excessive energy input, by backreflection of
light into the bulb or by improper cooling) or due to use above rated
lifetime.
Therefore we got back to the microscope suppliers and this is what we found:
Explosions have been reported on Zeiss microscopes. In old equipment, lamp
explosion might be caused by a slightly misaligned optical system, i.e. when
the backreflected light (from the rear mirror) is going back directly into
the electrode area of the lamp. This might cause overheating of the lamp with
a reduced lifetime or explosion.
In Nikon microscope: We did some testing on lamp housing from Nikon with HBO
103W/2. Here we found, that in some cases the measured temperature was close
to the upper level of the tolerated temperature, but still within the given
specs.
From users it was also reported, that contact between lamp and lamp holder
might create problems in a very few cases due to improper fitting. This might
result in local overheating ( the metal on the lamp base gets a bluish color)
and cracking the lamp. The cracking of the lamp might be proceeded by a
flickering, caused by arcing between lamp and lamp holder. If you find your
bases with a blue color, please report to your Nikon dealer or service for
inspection of the lamp holder. Under proper contact conditions, no explosion
will happen.


Statement NR. 3 will follow soon and contains information about start up
time.




From: OSRAMWG-at-aol.com
Date: Mon, 14 Oct 1996 18:31:03 -0400
Subject: HBO 50 W/AC, HBO 100 W/2, HBO 103 W/2, lamp for microscopes:FAQ's

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STATEMENT Nr. 5

issued by OSRAM GmbH, Photo-Optics Marketing, Berlin
Fax: (Germany)-30 -3386 -2773, e-mail: osramwg-at-aol.com

re: HBO 50 W/AC, HBO 100 W/2 and HBO 103 W/2, lamp for microscopes: FAQ's

Dear subscribers,

this is now the last statement NR. 5, related to frequently asked questions.

1.) What are the technical date for my lamp?
Please send an e-mail or a fax with your address- we will get you the latest
datasheets.

2.) Can I replace my HBO 100/2 by the longlife version HBO 103/2?
In principle...You can however.... The electrical characteristics are about
the same. In addition, we have tested all lamps in Zeiss, Nikon etc.
equipment available on the market. No problems have been found or reported
from customers so far.
However...
- for old Nikon equipment we currently recommend the HBO 100W/2 since there
is a better coupling between power supply and lamp due to the lower lamp
voltage.
- for Zeiss equipment: please ask your Zeiss service about the optical
design in your particular lamp house. Zeiss has changed their optical setup a
certain time ago- new equipment therefore should not see any problems.

3.) My HBO 50 W/AC failed after about 50 h to ignite (cool room due to air
condition) , I used a hair dryer, warmed up the lamp and than it started
again- does this damage the lamp?
The hair dryer either evaporates a small portion of the mercury from the
surface of the electrode inside the lamp and makes it easier for the cold
lamp to start. Or it heats up the bulb connections (see statement NR. 3) A
moderate heating will not damage the lamp ( I do not know what happens to
the lamp housing and the paint.), however this should not be the way for a
microscopist to work with OSRAM lamps. I assume, that this was either one of
the lamps, mentioned in statement NR. 1 or it was a contact problem like we
have seen it from Nikon equipment). In the first case, report to your OSRAM
dealer or to us directly (include Serial number and type of microscope), we
will replace your lamp. In the second case, you should report to your
microscope dealer.

4.) Can I install an additional fan to reduce risk of explosion and to
increase lifetime?
We do not recommend to do this because:
- under normal circumstances you should not have an explosion, please refer
to previous statement NR. 2 about possible reasons for an explosion.
- cooling of the lamp will reduce output power due to condensation of the
mercury
- cooling of the lamp might create tensions in the bulb and cause and
explosion

5.) If my lamp (HBO 50 W/AC) in my ZEISS microscope does not ignite, I was
told to press a special button.
This "special button" is the reset button for the igniter. The starter
disables after a certain number of unsuccessful attempts to ignite the lamp
or when the current has exceeded a certain value. If there is no ignition,
please press this little button. Refer to the Zeiss users manual!

6.) My microscope service told me, that according the newest research there
is absolutely no reason for the two images not to be aligned after lamp
replacement.
In general, this is not correct and might damage your system. Please refer to
your handbook or ask your microscope supplier about the proper alignment.


This was the last statement on the recently reported problems on HBO lamps
for microscopes. Please feel free to contact us directly in case you have any
further questions: osramwg-at-aol.com




From: rigbyj4-at-cs.uleth.ca (Joel Rigby)
Date: Tue, 15 Oct 1996 10:56:40 -0600
Subject: SEM and It's Use in Microcircuitry Applications

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I am a Student at the University of Lethbridge and I am taking a
Microscopy course related to the study of Microcircuitry. My professor is a
biological Microscopist and has rather limited experience with the study of
circuitry. As this is the case I was wondering if anyone on this list has
suggestions for things to study related to this and any experiments that might
be interesting.

Thank you all
Joel Rigby




From: N. Shashidhar :      NSHASHIDHAR-at-intergate.dot.gov
Date: Tue, 15 Oct 1996 12:32:16 -0400
Subject: Quantitative microscopy of packed particles

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I need to quantify pavements, which can be looked at as a system of packed particles. I need to
obtain the number of contacts each particle has on average with another particle. This can then be
related to mechanical properties such as shear resistance, etc.

Are there microscopic techniques to get this information from a compact and stereology methods to
extract such information. Any response will be appreciated.

Thank You.





From: nzjaba-at-madison.tec.wi.us
Date: Tue, 15 Oct 1996 14:00:46 -0500
Subject: Use of EM in forensic science

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My name is Nancy Zjaba and I am a student in the Madison (Wis.) Area
Technical College EM program. As part of my coursework, I am required to
give an oral report. My topic is the use of EM in forensic science. I have
done preliminary research, but have specific questions I'm hoping someone
will help me with (or point me to a reference):

1. When did SEM and TEM first pass the Frye rule as acceptable
techniques for examining evidence?

2. How is it that the courts have limited the kind of sample
preparation that can be done? What common preparation techniques can be
used, and which ones can't?

3. Are people who conduct forensic EM required to testify in court?
How often? Does maintaining the "chain of evidence" require a lot of time
(as far as paperwork)?

Thanks in advance for your help!

Nancy Z.





From: wcornell-at-centum.utulsa.edu (Winton Cornell)
Date: Tue, 15 Oct 1996 14:18:22 -0500
Subject: Freebie

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Scopers:

We were cleaning a closet when we found a brandy new (we think) CRT for a
Cambridge 600. (host instrument retired here in 1990) Specs on the box
read as follows: Thorn Brimar limited, Type M17/151BE, serial No. 430142.
Why let you know? Well, it's available....for free!

Now, not having shipped one of these babies before, I note that there is a
cautionary note on the (original) packing about the contents being:
"glass", "evacuated tube", etc. Do any of you know if there are any
restrictions on mailing a CRT?

Qualifications for donation: not exactly a first come - first
serve...rather, a first "needy" come - first serve. Please mail me with
your requests (if there be any at all) and I will choose a winner.

Winton Cornell

P.S. I will need a FedEx # or like for the mailing


Dr. Winton Cornell
Senior Research Associate
Department of Geosciences
The University of Tulsa
600 South College
Tulsa, OK 74104-3189

phone: 918-631-3248
fax: 918-631-2091
e-mail: wcornell-at-centum.utulsa.edu






From: shaf :      mshaf-at-darkwing.uoregon.edu
Date: Tue, 15 Oct 1996 14:12:52 -0700
Subject: Re: Use of EM in forensic science

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At 02:00 PM 10/15/96 -0500, Nancy Zjaba wrote:
} My name is Nancy Zjaba and I am a student in the Madison (Wis.) Area
} Technical College EM program. As part of my coursework, I am required to
} give an oral report. My topic is the use of EM in forensic science. ...

This is an interesting topic ... please reply to the list ... TIA.

cheers, shaf

{\/} /\ {\/} /\ {\/} /\ {\/} cognito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/}
Michael Shaffer, R.A. - University of Oregon Electron Probe Facility
mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu
http://darkwing.uoregon.edu/~mshaf/epmahome/





From: Dave Teter :      teter-at-lanl.gov
Date: Tue, 15 Oct 1996 12:40:02 -0600
Subject: Negative film scanners

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I am considering purchasing a negative film scanner and I have narrowed the
choices to the Polaroid Sprintscan 45, the Nikon LS4500AF or the LeafScan
45. The LeafScan 45 is no longer produced but I may be able to get a hold of
one of the last ones or a rebuilt one. I have used the LeafScan 45, but I
have no experience with either the Polaroid or the Nikon. The LeafScan
exceeds the performance of the other two (according to the specifications),
but is the cost justified? The Polaroid and Nikon are approximately half the
price of the LeafScan.

I would appreciate hearing your comments (advantages/disadvantages) if you
have used either the Polaroid or the Nikon, especially if you have
experience with the LeafScan for comparison purposes.

Thanks in advance.
*******************************************************
David F. Teter
Los Alamos National Laboratory
Materials Science and Technology: Metallurgy (MST-6)
Mail Stop: G755
Los Alamos, NM 87545
ph: (505)665-0160 fax: (505) 665-0657
e-mail: teter-at-lanl.gov
*******************************************************





From: Probing & Structure :      p&s-at-ultra.net.au
Date: Wed, 16 Oct 1996 21:04:48 +1000
Subject: Re: Negative film scanners - alternative

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At 12:40 15/10/96 -0600, you wrote:
}
} I am considering purchasing a negative film scanner and I have narrowed the
} choices to the Polaroid Sprintscan 45, the Nikon LS4500AF or the LeafScan
} 45. The LeafScan 45 is no longer produced but I may be able to get a hold of
} one of the last ones or a rebuilt one. I have used the LeafScan 45, but I
} have no experience with either the Polaroid or the Nikon. The LeafScan
} exceeds the performance of the other two (according to the specifications),
} but is the cost justified? The Polaroid and Nikon are approximately half the
} price of the LeafScan.
}
} I would appreciate hearing your comments (advantages/disadvantages) if you
} have used either the Polaroid or the Nikon, especially if you have
} experience with the LeafScan for comparison purposes.
} Thanks in advance.
} *******************************************************
} David F. Teter
} Los Alamos National Laboratory
} Materials Science and Technology: Metallurgy (MST-6)
} Mail Stop: G755
} Los Alamos, NM 87545
} ph: (505)665-0160 fax: (505) 665-0657
} e-mail: teter-at-lanl.gov
} *******************************************************
David & all:
I have an interest in digital cameras - we sell the MicroLumina in
Australasia. However, I truly believe that a high resolution digital camera,
although more expensive, is a better solution than a scanner. I don't like
scanners, I've had too much trouble with scan lines etc.
Combined with a macro lens a digital camera can copy negs on a light
box beautifully, it can also "copy" prints, apparatus, people and record
microscope images. In short it's infinitely more versatile.
Jim Darley

Probing & Structure
} (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia
}
} Phone +61 77 740 370 Fax: +61 77 892 313
} A great microscopy site http://www.ultra.net.au/~pns/





From: Wayne England :      wengland-at-ortech.on.ca
Date: Wed, 16 Oct 1996 08:53:00 -0400
Subject: count rate fluctuations

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Hello All,
We are having a problem that I wondered if anyone has had experience with
out there.
We actually have two problems that may or may not be associated. We have
been getting up to 5% Sr detected when doing quantitative analysis with
specimens that are high in Si (~20%) where no peaks for Sr are detected on
the spectra (even at 35kv) and there should be no Sr present in the sample.
The samples are charging at times due to the high Si but this is not always
the case. We are concerned about the proximity of the peaks causing some
type of overlap for some reason (Si and Sr)

We are also getting count rate variations with alterations in contrast and
brightness settings while in both back scatter and secondary modes
respectively. These alterations are not having an effect on our total area
readings but they do influence the count rate and dead time.

We have tried conditioning the analyzer, multiple calibrations, changing
electrical outlets for the back scatter detector, carbon coating the
specimens... We also noted recently that our backscatter detector had
slipped and was almost touching the secondary detector though this has been
corrected and is no longer a problem.
Analysis of copper/gold standards are giving good quantitative results.

I realize that these details are somewhat sketchy but does anyone recognize
this problem? Any input is greatly appreciated.

Wayne England
ORTECH CORP.
Mississauga, Ontario
wengland-at-ortech.on.ca







From: houpt-at-worldxs.worldaccess.nl (houpt)
Date: Wed, 16 Oct 1996 15:48:58 +0100
Subject: relay optics

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Dear microscopists listers,

I like to know how I can make a relay lenssystem for connecting a CCD
camera to the camera port of a lightmicroscope.
I want to see 1/3 to 3/4 of the visual field on the monitor.

Thank you for help,

Pieter Houpt

BIOMET-applied phycology.
biological microscopy.







From: wise-at-vaxa.cis.uwosh.edu
Date: Wed, 16 Oct 1996 09:28:45 +0000
Subject: Formaldehyde fixation

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I got a "Stump The Prof" question today. If formaldehyde is a
monofunctional fixative, how can it cross-link anything and, by extension,
how can it be a fixative at all?

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-uwosh.edu






From: Anthony J. Garratt-Reed :      tonygr-at-MIT.EDU
Date: Wed, 16 Oct 1996 09:22:36 -0400
Subject: Freebie...

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Referring to Winton Cornell's offer of a free M17/151BE CRT, VG HB5/HB501
owners may like to check on the part numbers of their CRT's - I've a feeling
they are the same. But then, my memory may fail me!

Tony Garratt-Reed


*********************************
** **
** Anthony J. Garratt-Reed **
** MIT Room 13-1027 **
** 77 Massachusetts Avenue **
** Cambridge, MA 02139-4307 **
** USA **
** **
** Ph: 617-253-4622 **
** Fax: 617-258-6478 **
** **
*********************************
*********************************





From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Wed, 16 Oct 1996 12:38:32 -0500
Subject: Re: Formaldehyde fixation

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Message-Id: {199610161349.IAA05368-at-Sparc5.Microscopy.Com}
To: Microscopy messageslistserver {Microscopy-at-Sparc5.Microscopy.Com}

} I got a "Stump The Prof" question today. If formaldehyde is a
} monofunctional fixative, how can it cross-link anything and, by extension,
} how can it be a fixative at all?
}
} Bob

From J.A. Kiernan, _Histological & Histochemical Methods_, pg 18ff:

formaldehyde reacts with water to form methylene hydrate HOCH2OH this
then reacts with various parts of proteins to form methylene cross-links.
But I've also heard that it works by a two-stage reaction, where 2
CHO's react to form some unspecified product with two functional groups
which then cross-link the proteins.
I prefer the first explanation.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel *all mail:*
University of Illinois Station A
Rm 74 Bevier Hall PO Box 5037
905 S. Goodwin Ave. Champaign, IL 61825-5037 USA
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu
(217) 355-1143 home

*********** looking for a job again ***********






From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Wed, 16 Oct 1996 12:57:46 -0500
Subject: Re: Formaldehyde fixation

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This puzzled me once also but I think I now understand.

RH + CH2O {=} RCH2(OH)

Then

RCH2(OH) + HR' {=} RCH2R' + H2O

note that this is a reversible reaction so with extensive washing one can
lose the fixation. could this be how some "antigen retrival" processes
work?

Most of the formaldehyde in aqueous solution forms a glycol called
methylene glycol. the small formaldehyde penetrates the tissue rapidly.
as the formaldehyde reacts with tissue, it shifts the equilibrium and more
methylene glycol turns into formaldehyde. this latter transition is one
reason formaldehyde is a slow fixer despite being a rapid pentetrator.

Also with time, polymers of formaldehyde form and can substitute directly
into this equation. This is why older stocks of formalin or formaldehyde
can act differently from fresh stocks.

Hope this helps. Tom Phillips.


} I got a "Stump The Prof" question today. If formaldehyde is a
} monofunctional fixative, how can it cross-link anything and, by extension,
} how can it be a fixative at all?
}
} Bob
}
}
} Robert R. Wise, PhD
} Director, UWO Electron Microscope Facility
} Department of Biology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} (414) 424-3404 tel
} (414) 424-1101 fax
} wise-at-uwosh.edu

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(573)-882-4712 (voice)
(573)-882-0123 (fax)






From: winey-at-lrsm.upenn.edu (Karen I. Winey)
Date: Wed, 16 Oct 1996 12:08:27 -0500
Subject: Postdoctoral Position

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Posted-Date: Wed, 16 Oct 1996 12:03:55 -0400 (EDT)
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Post Doctoral Position

Department of Materials Science and Engineering &
Laboratory for Research on the Structure of Matter
3231 Walnut Street
University of Pennsylvania
Philadelphia, PA 19104-6272

Qualified candidates will have a Ph.D. in a physical science or engineering
field as well as considerable experience with transmission electron
microscopy. Preferred candidates will have experience with beam sensitive
materials, EELS, and/or polymer microscopy. The successful candidate will
initiate two or more new projects associated with (1) lateral uniformity at
polymer interfaces, (2) electrically active polymeric devices, and/or (3)
optically active polymers. These projects will involve the (soon to arrive)
field emission analytical TEM.


Start Date is flexible.
Earliest start date: December 1, 1996
Latest start date: April 1, 1996


To apply please send the following items to the address above:
1. complete curriculum vitae
2. names and addresses of two references
3. one preprints/reprints


Applications are due November 20, 1996.

regards,
Prof. Karen I. Winey
winey-at-lrsm.upenn.edu




-----------------------------------------------------------------------------
Karen I. Winey
Assistant Professor (215) 898-0593
Materials Science and Engineering Department (215) 573-2128 FAX
3231 Walnut Street winey-at-lrsm.upenn.edu
University of Pennsylvania LRSM building; room 308
Philadelphia, PA 19104-6272






From: winey-at-lrsm.upenn.edu (Karen I. Winey)
Date: Wed, 16 Oct 1996 12:08:27 -0500
Subject: Postdoctoral Position

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Posted-Date: Wed, 16 Oct 1996 12:03:55 -0400 (EDT)
X-Sender: winey-at-sol1.lrsm.upenn.edu
Message-Id: {v01540b02ae8ac483d66d-at-[130.91.56.135]}
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Post Doctoral Position

Department of Materials Science and Engineering &
Laboratory for Research on the Structure of Matter
3231 Walnut Street
University of Pennsylvania
Philadelphia, PA 19104-6272

Qualified candidates will have a Ph.D. in a physical science or engineering
field as well as considerable experience with transmission electron
microscopy. Preferred candidates will have experience with beam sensitive
materials, EELS, and/or polymer microscopy. The successful candidate will
initiate two or more new projects associated with (1) lateral uniformity at
polymer interfaces, (2) electrically active polymeric devices, and/or (3)
optically active polymers. These projects will involve the (soon to arrive)
field emission analytical TEM.


Start Date is flexible.
Earliest start date: December 1, 1996
Latest start date: April 1, 1996


To apply please send the following items to the address above:
1. complete curriculum vitae
2. names and addresses of two references
3. one preprints/reprints


Applications are due November 20, 1996.

regards,
Prof. Karen I. Winey
winey-at-lrsm.upenn.edu




-----------------------------------------------------------------------------
Karen I. Winey
Assistant Professor (215) 898-0593
Materials Science and Engineering Department (215) 573-2128 FAX
3231 Walnut Street winey-at-lrsm.upenn.edu
University of Pennsylvania LRSM building; room 308
Philadelphia, PA 19104-6272






From: Warren Straszheim :      wes-at-ameslab.gov
Date: Wed, 16 Oct 1996 10:23:01 -0500
Subject: Re: count rate fluctuations

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Why is Sr even showing up in your analyses? I know we can help or hurt our
standardless results by confusing the element list. I would rather not let
the analyzer try to figure out which elements are present, or if it tries to
revise the list to the proper elements. (I got very tired of always deleting
V from the list of identified elements when O was present.) Without
standards, a certain amount of Sr may help the fit to your Si peak. It
depends on how good a shape they use. This is another arguement for using
standards whenever possible - both a proper peak shape and a real intensity
reference.

Regarding the fluctuating count rate - Are you running a "shotgun" analysis
over an area at a "slow" scan rate on a heterogenous sample? We set the
count rate for x-ray maps on concrete and watch the count rate wander 10%
and more as the beam scans the field. We are now setting it using TV scan
rate, although it doesn't matter a lot except for consistency.

At 08:53 AM 10/16/96 -0400, you wrote:
}
} Hello All,
} We are having a problem that I wondered if anyone has had experience with
} out there.
} We actually have two problems that may or may not be associated. We have
} been getting up to 5% Sr detected when doing quantitative analysis with
} specimens that are high in Si (~20%) where no peaks for Sr are detected on
} the spectra (even at 35kv) and there should be no Sr present in the sample.
} The samples are charging at times due to the high Si but this is not always
} the case. We are concerned about the proximity of the peaks causing some
} type of overlap for some reason (Si and Sr)
}
} We are also getting count rate variations with alterations in contrast and
} brightness settings while in both back scatter and secondary modes
} respectively. These alterations are not having an effect on our total area
} readings but they do influence the count rate and dead time.
}
} We have tried conditioning the analyzer, multiple calibrations, changing
} electrical outlets for the back scatter detector, carbon coating the
} specimens... We also noted recently that our backscatter detector had
} slipped and was almost touching the secondary detector though this has been
} corrected and is no longer a problem.
} Analysis of copper/gold standards are giving good quantitative results.
}
} I realize that these details are somewhat sketchy but does anyone recognize
} this problem? Any input is greatly appreciated.
}
} Wayne England
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Laszlo Veto :      VetoL-at-em.agr.ca
Date: Wed, 16 Oct 1996 18:14:53 -0400
Subject: Re: Carbon Contamination rates -Reply

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Message-Id: {s2652602.050-at-EM.AGR.CA}
X-Mailer: Novell GroupWise 4.1

** High Priority **

Glenn,

This classical paper may interest you also:
"Current efforts to attain the resolution limit of the transmission electron
microscope" by E. Ruska, Journal of the Royal Microscopical Society,
Vol. 84, Pt. 1, April 1965. Pp. 77-103.

Enjoy!

Laszlo J. Veto
PARC




From: Arthur Gillman :      ARGIL-at-delphi.com
Date: Wed, 16 Oct 1996 19:12:52 -0500 (EST)
Subject: relay optics

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to Pieter Houpt, who asks about relay optics to connect a c-mount camera
to a camera port:

We have mounted various cameras on the ports of many microscopes, and the
general conclusion is that they are all different. The usual relay optic
is used to step the real image from the port down to the size of the
camera sensor. The details of a design depend on the sensor size and the
position of the image when the microscope is in focus. I have seen systems
as simple as a singlet lens (not very satisfactory) to a singlet and
doublet combination (worked well). To a large extent, the difficulties
are mechanical.

We actually sell a relay lens that fits into a DIN 23mm eyepiece hole,
with or without a clamp assembly, and has a c-mount thread on
the other end. We use it with many of our systems. If you would like
more info, Email me.

I can also give you the name of a company that makes camera adapters for most
of the standard light microscopes.

Arthur Gillman
Princeton, NJ







From: r.g.white-at-sci.monash.edu.au (Rosemary White)
Date: Thu, 17 Oct 1996 09:56:24 +1200
Subject: Re: Formaldehyde fixation

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} I got a "Stump The Prof" question today. If formaldehyde is a
} monofunctional fixative, how can it cross-link anything and, by extension,
} how can it be a fixative at all?
}
Isn't formaldehyde cross-linked to itself to some extent - i.e. once made
up into solution it's in small polymers not pure monomers? I think this is
the case even for solutions made from paraformaldehyde powder. And the C
in HCHO should be able to cross-link two Ns from different amino groups or
peptide links. Acrolein is also a monoaldehyde, I think, but is a very
efficient fixative so must work in a similar way. I'm not 100% sure about
this, and am no chemist, just remember it from long ago EM prep classes.

Rosemary

Rosemary White
Department of Ecology and Evolutionary Biology
Monash University, Clayton, Victoria 3168, Australia
phone 61-3-9905 5670 email r.g.white-at-sci.monash.edu.au
fax 61-3-9905 5613 or rgwhite-at-vaxc.cc.monash.edu.au






From: caa3045-at-ritvax.isc.rit.edu (Ciprian Almonte)
Date: Thu, 17 Oct 1996 00:17:30 -0400
Subject: Re: Negative film scanners (fwd)

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}
} I am considering purchasing a negative film scanner and I have narrowed the
} choices to the Polaroid Sprintscan 45, the Nikon LS4500AF or the LeafScan
} 45.
David, if you have the money, I will definitely recommend you
purchasing the LeafScan 45. I have been using the LeafScan 45 for the last
two years, and I'm very happy with it. The resolution and reliability of
the scan are more superior than the Polaroid Sprintscan, and the Nikon
LS4500AF.

--Ciprian

_________________________________________________________
Ciprian A. Almonte
Rochester Institute of Technology
Biomedical Photographic Communications
Rochester, NY 14623-5603

Visit my web site at http://www.isc.rit.edu/~caa3045/
__________________________________________________________






From: Reinhard Rachel t4534 :      reinhard.rachel-at-biologie.uni-regensburg.de
Date: Thu, 17 Oct 1996 09:26:46 +0200
Subject: Re: Formaldehyde fixation

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"Formaldehyde fixation" (Oct 16, 9:28am)
References: {01IAPF4IXY1E0044RK-at-vaxa.cis.uwosh.edu}
X-Mailer: Z-Mail (3.2.1 15feb95)
To: wise-at-vaxa.cis.uwosh.edu

For a recent book, which explains this and many other
problems in EM, check the monograph
Fine Structure Immunocytochemistry (ed: Gareth Griffiths, EMBL)
Springer, Berlin, 1993 (ISBN 3 540 54805 X)
or New York (ISBN 0 387 54805 X)
and many references cited therein. (pp. 39 ff on formaldehyde fixation)
Reinhard Rachel





From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Thu, 17 Oct 1996 08:15:15 +0100 (BST)
Subject: Re: Freebie

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Dear Winton;
Your message of Tuesday October is rather intriguing. You had written,
and I quote from your message "We were cleaning a closet when we found a
brandy----". I'm sure many of us as we read our e-mail at unsocial hours
would have had a sharp focus of interest which may have dropped away as
the message continued on about CRT etc. I hope you find a home for your
CRT.

Patrick Echlin

On Tue, 15 Oct 1996, Winton Cornell wrote:

} Scopers:
}
} We were cleaning a closet when we found a brandy new (we think) CRT for a
} Cambridge 600. (host instrument retired here in 1990) Specs on the box
} read as follows: Thorn Brimar limited, Type M17/151BE, serial No. 430142.
} Why let you know? Well, it's available....for free!
}
} Now, not having shipped one of these babies before, I note that there is a
} cautionary note on the (original) packing about the contents being:
} "glass", "evacuated tube", etc. Do any of you know if there are any
} restrictions on mailing a CRT?
}
} Qualifications for donation: not exactly a first come - first
} serve...rather, a first "needy" come - first serve. Please mail me with
} your requests (if there be any at all) and I will choose a winner.
}
} Winton Cornell
}
} P.S. I will need a FedEx # or like for the mailing
}
}
} Dr. Winton Cornell
} Senior Research Associate
} Department of Geosciences
} The University of Tulsa
} 600 South College
} Tulsa, OK 74104-3189
}
} phone: 918-631-3248
} fax: 918-631-2091
} e-mail: wcornell-at-centum.utulsa.edu
}
}
}





From: Woody.N.White-at-mcdermott.com
Date: 17 Oct 96 08:05:00 -0500
Subject: Re: count rate fluctuations

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Charging will affect SE much more than BSE, but I have certainly had specimens
charge to the point of disrupting BSE imaging. Charging will also reduce the
effective incident beam potential, causing possible loss of higher energy x-ray
counts. This will be evident when examining the x-ray background signal. With
no charging the bkgn should "tail-off" at the beam potential. As a specimen
charges, the maximum bkgn energy will become lower. For example, an incident
beam of 10kV will normally produce a background terminating at 10kV. If the
specimen has charged to 2kV, the bkgn will terminate at (10-2) 8kV.
Woody
______________________________ Reply Separator _________________________________
Subject: count rate fluctuations

The samples are charging at times ...SNIP...

We are also getting count rate variations with alterations in contrast and
brightness settings while in both back scatter and secondary modes
respectively. ...SNIP...

Wayne England
ORTECH CORP.
Mississauga, Ontario
wengland-at-ortech.on.ca




From: rgrappe-at-MMM.COM
Date: Thu, 17 Oct 1996 08:55:04 -0500
Subject: Project Micro

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The Minnesota Microscopy Society is involved in a preliminary trial of
the Project Micro Program. One of the experiments calls for examining
various sands at low magnification (10x-20x). These sands should have
unique morphological or color properties which could be seen at this
magnification. If anyone has access to some unique sand we would
appreciate the help. Please contact me at my EMail address

RGRappe-at-mmm.com

and we can make arrangements on how obtain it.

Thanks in advance.

Rod Rappe




From: levin-at-ecsuc.ctstateu.edu (Martin Levin)
Date: Thu, 17 Oct 1996 08:38:24 -0500
Subject: Hummer V Sputter Coater

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Dear Microscopists,

I am thinking about buying a used Hummer V Sputter Coater. To those
unfamiliar with the model, it is a completely self-contained unit (i.e., it
has an integral vacuum pump) and is also capable of carbon coating. The
instrument is about 8 years old, but has hardly seen any use. It basically
looks brand new and comes with a gold-palladium source and a carbon source.
It has been cleaned, greased, oiled, etc., and it works well. I have two
questions:

1. Is this a good, reliable, instrument?

2. Is $2500 a fair asking price.

Thanks.

Martin A. Levin
Department of Biology
Eastern Connecticut State University
Willimantic, CT 06226
Phone: (860)465-4324 FAX: (860)465-5213






From: Wayne England[SMTP:wengland-at-ortech.on.ca]
Date: Thu, 17 Oct 1996 10:18:03 -0400
Subject: count rate fluctuations

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Message-Id: {01BBBC14.7BACA360-at-summer.geol.sc.edu}

Wayne:
It is not clear if you are using WDS or EDS or which lines you are =
measuring but regardless, the Si K-beta line is close enough to Sr =
L-alpha to cause spurious counts for Sr, even with the better-resolving =
WDS technique. My experience on a microprobe with wavelength =
spectrometers is that Sr measurement below ~ 0.5 wt% SrO in a silicate =
with ~50 wt% SiO2 was quite difficult unless some overlap corrections =
are made. =20
Your description of count rate variations in BSE or SE mode is =
difficult to understand, but if you are collecting element intensity =
data while scanning a heterogeneous target, of course you will get =
fluctuations. If Si varies in your sample, then the Sr data due to Si =
overlap will also vary. Did I understand your problem correctly?
-Jim McGee
*.*.*.*.*.*.*.*.*.*.*.*.*.*.*.*.*.*.*.*.*
James J. McGee (jmcgee-at-sc.edu)
Dept. of Geological Sciences
University of South Carolina (803) 777-6300 (Office)
Columbia, SC 29208 (803) 777-6610 (Fax)


----------


Hello All,
We are having a problem that I wondered if anyone has had experience =
with=20
out there.
We actually have two problems that may or may not be associated. We =
have=20
been getting up to 5% Sr detected when doing quantitative analysis with=20
specimens that are high in Si (~20%) where no peaks for Sr are detected =
on=20
the spectra (even at 35kv) and there should be no Sr present in the =
sample.=20
The samples are charging at times due to the high Si but this is not =
always=20
the case. We are concerned about the proximity of the peaks causing =
some=20
type of overlap for some reason (Si and Sr)

We are also getting count rate variations with alterations in contrast =
and=20
brightness settings while in both back scatter and secondary modes=20
respectively. These alterations are not having an effect on our total =
area=20
readings but they do influence the count rate and dead time. ...


Wayne England
ORTECH CORP.
Mississauga, Ontario
wengland-at-ortech.on.ca








From: peling-at-amnh.org (Peling Melville - Interdepartmental Facilities)
Date: Thu, 17 Oct 1996 14:52:37 -0500
Subject: BSE Detectors for SEM

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Dear Microscopists,

I was wondering about the kinds of BSE detector systems that people have
with their SEM's. What are your opinions? How reliable is the detector
system that you have? Which one/one's would you recommend?

Thanks in advance,
Peling Melville

--------------------------------------------------------------
Peling Fong Melville
Senior Scientific Assistant
Interdepartmental Facilities
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 U.S.A.
******************************
E-mail: peling-at-amnh.org
Work #: (212) 769-5469
FAX #: (212) 769-5495






From: rcrang-at-pop.life.uiuc.edu (Richard Crang)
Date: Thu, 17 Oct 1996 14:30:17 +0700
Subject: Identifying heavy metals in low concentration

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On of the researchers here is doing toxicologic research on marine mammmal
specimens collected in a field situation. Specifically, she is concerned
with the localization of heavy metal contaminants in tissues at a cellular
and subcellular level. Tissues will be analyzed for metal concentrations
via atomic absorption spectroscopy
(AA). In addition, autometallographic silver development of tissue slide
preparations at the light and electron microscopic level will be used to both
amplify and localize metal deposition in tissue. However, she is looking for a
technique that would positively identify minute amounts of metals in slide
preparations that would serve as an adjunct to autometallographic and AA
results. The elements of particular interest are mercury, silver, and
selenium. I am anticipating that concentrations in tissues will range from
limits of detection (for AA) to possibly a few hundred ppm, with most
concentrations being in the 5-50 ppm range, thus very likely to be outside
usual EDX limits. Is PIXE a useful adjunct technique? Any advice with
respect to a technique designed to identify these elements at low
concentration in situ would be greatly appreciated.


************************
Richard Crang
Dept. Plant Biology
University Illinois
Urbana, IL 61801
(217) 244-3143
************************






From: Dave King (607)757-1248 T37/257-3 Ext deking-at-vnet.ibm.com
Date: 17 Oct 1996 16:50:19 EDT
Subject: Hummer V

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Message-Id: {199610172103.QAA08710-at-Sparc5.Microscopy.Com}

To: Microscopy ListSer {Microscopy-at-MSA.Microscopy.Com}



We've been using a Hummer IV for about 14 years, and it's done
very well. There were vacuum leak problems, but we sorted them
out. Price? No idea, sorry.

. {
} { {
} } ===========} Dave King { { {
} } } ------------------------------------------------------ { { { {




From: Woody.N.White-at-mcdermott.com
Date: 10/17/96 8:55 AM
Subject: Project Micro

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Would you be interested in sand collected by my well pump filter? The
grains are sharp edged and irregular - about 100 microns long. The
sand is predominantly an even mix of quartz (clear/white) and nearly
black particles. I forget the composition of the black - I think it
was comprised of O, Si, Fe, and maybe(?): Mg, Ca, Mn, and or Ti??
A geologist friend belives it comes from decomposing (poorly
"sintered") igneous(sp?) rock which was once an undersea deposit of
volcanic ash.

Woody White
Electron Microscopist
Wk: woody.n.white-at-mcdermott.com
Hm: woody.white-at-worldnet.att.net
http://www.geocities.com/capecanaveral/3722


______________________________ Reply Separator _________________________________


Organization: 3M
X-Mailer: Mozilla 2.01 (Win95; I)
Mime-Version: 1.0
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The Minnesota Microscopy Society is involved in a preliminary trial of
the Project Micro Program. One of the experiments calls for examining
various sands at low magnification (10x-20x). These sands should have
unique morphological or color properties which could be seen at this
magnification. If anyone has access to some unique sand we would
appreciate the help. Please contact me at my EMail address

RGRappe-at-mmm.com

and we can make arrangements on how obtain it.

Thanks in advance.

Rod Rappe




From: CrushStone-at-aol.com
Date: Thu, 17 Oct 1996 19:03:00 -0400
Subject: Re: Project Micro

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Rod:

You absolutely should include sand from Colorado Silica. There are many
small agates in this sand, it is beautiful!!

Steve Stokowski
Stone Products Consultants
508-881-6364




From: Donna Jacobs :      jacobs-at-uimrl7.mrl.uiuc.edu
Date: Thu, 17 Oct 1996 16:44:34 -0500
Subject: Open Position - Research Electron Microscopist

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University of Illinois at Urbana-Champaign
Materials Research Laboratory


RESEARCH ELECTRON MICROSCOPIST


The Materials Research Laboratory at the University of Illinois is seeking
an experienced electron microscopist as a staff member in the Center for
Microanalysis of Materials. The Center is a major research facility with
eight electron microscopes as well as instruments in surface microanalysis,
x-ray diffraction, and other analytical techniques.

The person will work mainly on SEM, but should have the experience and
flexibility to work also in TEM and possibly other techniques, if needed.
The Center has two SEMs and a third may be acquired shortly. Experience in
one or more of the following techniques would be especially important:
Cathodo-luminescence, EBSP, quantitative EDX. The main responsibilities of
the position are to facilitate the research of the approximate 150 SEM users
yearly, train new users, and keep the instruments running.

There will be ample opportunity to carry out interactive research in the
facility with the wide range of research programs. Possibilities for
independent research could also be developed.

This position requires a university degree in physics, materials science or
a related field (PhD is desired) and at least three years experience with
electron microscopes.

This is a 12 month, 100% time regular appointment with standard university
benefits. Salary will be commensurate with education and experience. The
position will be available in early 1997. In order to ensure full
consideration, application must be received by November 30, 1996. Please
send letter of application, resume, and names and addresses of three
references to J. A. Eades, c/o Donna Jacobs, University of Illinois,
Materials Research Laboratory, 104 South Goodwin Avenue, Urbana, Illinois
61801, phone (217) 244-2944. For technical information, call J. A. Eades at
(217) 333-8396.

The University of Illinois is an Affirmative Action/Equal Opportunity
Employer.





From: r.g.white-at-sci.monash.edu.au (Rosemary White)
Date: Fri, 18 Oct 1996 10:33:54 +1200
Subject: bacterial biofilms

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Dear all,

A colleague would like to study the development of polysaccharide biofilms
(secreted by Pseudomonad bacteria) on lettuce leaves. She asked whether
there were any quick and easy (or, if necessary, long and tricky) ways of
seeing whether these polysaccharides were present on leaves before and
after they are turned into salads. My suggestion was to use a simple
polysaccharide stain on hand sections of leaves, but the film may be too
thin to distinguish it from the cuticle, even if she compared leaves from
sterile cultures with field-grown leaves. Any suggestions?

TIA,

Rosemary White
Department of Ecology and Evolutionary Biology
Monash University, Clayton, Victoria 3168, Australia
phone 61-3-9905 5670 email r.g.white-at-sci.monash.edu.au
fax 61-3-9905 5613 or rgwhite-at-vaxc.cc.monash.edu.au






From: manuela :      manuelap-at-petermac.unimelb.edu.au
Date: Fri, 18 Oct 1996 11:30:07 +1000
Subject: Evans Blue

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Hi,

Could someone tell me what it is exactly that Evans Blue binds to in a cell????
Does anyone know?

Many thanks

M.Palatsides





From: geoffa-at-amsg.austmus.gov.au (GeoffA)
Date: 17/10/96 2:52 PM
Subject: BSE Detectors for SEM

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Mime-Version: 1.0
peling-at-amnh.org (Peling Melville - Interdepartmental Facilities)
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
Content-Description: cc:Mail note part

Hi Peling,

IMHO there's only one choice, the Robinson BSE detector. We do approx. 90%
of our micrographs using ours and it's worked wonderfully for 10 years.
The decision becomes 'which model of Robinson to get'?

Cheers,

::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
Geoff Avern
Manager
Microscopy Laboratories
Australian Museum Email: geoffa-at-amsg.austmus.oz.au
6 College St Ph: (61)(2) 9320 6198
Sydney, Australia. 2000 Fax: (61)(2) 9320 6059
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
______________________________ Reply Separator _________________________________


Dear Microscopists,

I was wondering about the kinds of BSE detector systems that people have
with their SEM's. What are your opinions? How reliable is the detector
system that you have? Which one/one's would you recommend?

Thanks in advance,
Peling Melville

--------------------------------------------------------------
Peling Fong Melville
Senior Scientific Assistant
Interdepartmental Facilities
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 U.S.A.
******************************
E-mail: peling-at-amnh.org
Work #: (212) 769-5469
FAX #: (212) 769-5495






From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Thu, 17 Oct 1996 21:01:48 +0100
Subject: Re: count rate fluctuations

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X-Sender: (Unverified)
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} Hello All,
} We are having a problem that I wondered if anyone has had experience with
} out there.
} We actually have two problems that may or may not be associated. We have
} been getting up to 5% Sr detected when doing quantitative analysis with
} specimens that are high in Si (~20%) where no peaks for Sr are detected on
} the spectra (even at 35kv) and there should be no Sr present in the sample.
} The samples are charging at times due to the high Si but this is not always
} the case. We are concerned about the proximity of the peaks causing some
} type of overlap for some reason (Si and Sr)
}
} We are also getting count rate variations with alterations in contrast and
} brightness settings while in both back scatter and secondary modes
} respectively. These alterations are not having an effect on our total area
} readings but they do influence the count rate and dead time.
}
} We have tried conditioning the analyzer, multiple calibrations, changing
} electrical outlets for the back scatter detector, carbon coating the
} specimens... We also noted recently that our backscatter detector had
} slipped and was almost touching the secondary detector though this has been
} corrected and is no longer a problem.
} Analysis of copper/gold standards are giving good quantitative results.
}
} I realize that these details are somewhat sketchy but does anyone recognize
} this problem? Any input is greatly appreciated.
}
} Wayne England
} ORTECH CORP.
} Mississauga, Ontario
} wengland-at-ortech.on.ca

Wayne,

Are you saying a) that the count rate changes as you adjust the SEM
controls for brightness/contrast while doing an analysis at a stationary
point or b) are you slowly scanning (or taking a series of multi-point
analyses) over an area and seeing the count rate change as the
brightness/contrast of the specimen image naturally changes?

If a), then you have a problem, but if b) then this is exactly what you
would expect - either the composition (if a flat polished surface) and/or
topography (if a rough surface) of the specimen is changing. Both will
affect the EDX count rate.

Any Sr giving rise to a L alpha peak that might confuse with Si would also
produce a much stronger K alpha/beta pair - as they don't show, you don't
have any Sr. Similarly M lines from heavier elements would also produce K
lines up around 8 keV, so I doubt that you have any other element genuinely
contributing to the spectra.

What count rates are you running at? If you have a high count rate, then an
escape peak from an element around 3.5keV might screw up the Si K peak, for
example, lots of Sb, Sn or Ca but as you don't mention these, I presume
this is not an issue.

You might have also have some real problems:

1. You may have a (maybe more than one) ground loop. Check that all your
ancilliary equipment is getting its power from the same source. Preferably,
this means connecting eveything to the auxillary power outputs of your SEM.
Also make sure that you don't have any spurious ground links - for example,
when the EDX detector is fully disconnected from the analyser, it should be
isolated from the SEM. If the detector is grounded both directly through
the SEM and via its own analyser, you will get odd things happening.

2. You say everthing is fine for Cu and Au standards. Are you using the Au
M and Cu L? You need to check using a reference with peaks in the same part
of the spectrum as the region you are having problems in since the problems
you are experiencing may not be simple, by which I mean, the effect may
have a complex relationship to energy, mainly affecting only the low engery
region of the spectrum. However, if these standards are genuine, nice flat
polished standards and the situation is as b) above, then this is exactly
what you would expect.

Hope that helps. If it raises more questions, come back to me.

Regards,

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
---------------------------------------------------------------






From: WARRENJ1-at-cliffy.polaroid.com
Date: 10/16/96 7:04 AM
Subject: Re: Negative film scanners - alternative

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OK, I'll jump in the middle of this. We have a digital camera also. We
have one of the referenced Film Scanners as well as three 35mm film
scanners. We also have flatbed scanners.

The bit depth,cost,dpi optical resolution, versatility and ease of use
of any of our scanners and others on the market outweigh any scan line
issues as well as the expense of getting the equivalent resolution and
bit depth of scanners.

John D. Warren
Southern Sales Manager "see what develops"
Digital Photographic Imaging Group
Polaroid Corporation

4525 Leonard Parkway
Richmond, Virginia 23221-1809
Office 804.254.1011
Fax 804.254.1013
Internet warrenj1-at-polaroid.com







______________________________ Reply Separator _________________________________


At 12:40 15/10/96 -0600, you wrote:
}
} I am considering purchasing a negative film scanner and I have narrowed the
} choices to the Polaroid Sprintscan 45, the Nikon LS4500AF or the LeafScan
} 45. The LeafScan 45 is no longer produced but I may be able to get a hold of
} one of the last ones or a rebuilt one. I have used the LeafScan 45, but I
} have no experience with either the Polaroid or the Nikon. The LeafScan
} exceeds the performance of the other two (according to the specifications),
} but is the cost justified? The Polaroid and Nikon are approximately half the
} price of the LeafScan.
}
} I would appreciate hearing your comments (advantages/disadvantages) if you
} have used either the Polaroid or the Nikon, especially if you have
} experience with the LeafScan for comparison purposes.
} Thanks in advance.
} *******************************************************
} David F. Teter
} Los Alamos National Laboratory
} Materials Science and Technology: Metallurgy (MST-6)
} Mail Stop: G755
} Los Alamos, NM 87545
} ph: (505)665-0160 fax: (505) 665-0657
} e-mail: teter-at-lanl.gov
} *******************************************************
David & all:
I have an interest in digital cameras - we sell the MicroLumina in
Australasia. However, I truly believe that a high resolution digital camera,
although more expensive, is a better solution than a scanner. I don't like
scanners, I've had too much trouble with scan lines etc.
Combined with a macro lens a digital camera can copy negs on a light
box beautifully, it can also "copy" prints, apparatus, people and record
microscope images. In short it's infinitely more versatile.
Jim Darley

Probing & Structure
} (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia
}
} Phone +61 77 740 370 Fax: +61 77 892 313
} A great microscopy site http://www.ultra.net.au/~pns/




From: N. Voster * Anatomiese Patologie * :      GNAPNV-at-med.uovs.ac.za
Date: Fri, 18 Oct 1996 11:24:37 GMT+2
Subject: Message

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Experienced diagnostic transmission electron microscopist, with SEM
experience too, looking for a job URGENTLY.


SUSAN COOPER

E-mail gnapnv-at-med.uovs.ac.za
Tel. 0027-51-4053061 (W)
0027-51-4366025 (H)

Fax 0027-51-4473222


#####################################################################
************************************************************
* Nellie Vorster - Anatomiese Patologie / *
* Anatomical Pathology *
* *
* Tel. : 051 - 405 3060 *
* Fax : 051 473222 *
* P.Mail: gnapnv-at-med.uovs.ac.za *
************************************************************





From: Henrik Kaker :      Henrik.Kaker-at-guest.arnes.si
Date: Fri, 18 Oct 1996 12:44:58 +0100
Subject: GW Instruments E-mail address

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Dear All,

I'm looking for E-mail address and fax number of GW Instruments, 35 St.
Medford, Sommerville, MA 02143.

Henrik
--
Henrik Kaker
SEM-EDS Laboratory, Metal Ravne d.o.o., Slovenia
Tel: +386-602-21-131, Fax: +386-602-20-436,
E-mail: Henrik.Kaker-at-guest.arnes.si
http://www2.arnes.si/guest/sgszmera1/index.html
Microscopy Vendors DataBase:
http://www2.arnes.si/guest/sgszmera1/vendors.html




From: ainswort-at-geology.gla.ac.uk (Pete Ainsworth)
Date: Fri, 18 Oct 1996 11:32:37 +0100
Subject: Re: BSE Detectors for SEM

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peling-at-amnh.org (Peling Melville - Interdepartmental Facilities)

Hi Peling,

I would agree with Geoff Avery that the Robinson detector will give you
better atomic number contrast than the four quadrant detectors but don't
discount the latter totally. We use a Kevex 4QBSE and have had extremely
good results from this. (in previous employment I used a Robinson
setector). The 4QBSE detector certainly achieves the manufacturers specs
and has given no problems in the time we have had it (6 years). When I used
the Robinson type detector an occassional anoyance was that on occassions I
had to remove the detector from the SEM in order to achieve high res SE
images on certain larger specimens.

I would say that a lot depends on what else you have and want. For example
we have a retractable CL detector and so could not use a Robinson without
physically swapping the detectors in the same port. (not a good idea)

Hope this helps

Pete

} Dear Microscopists,
}
} I was wondering about the kinds of BSE detector systems that people have
} with their SEM's. What are your opinions? How reliable is the detector
} system that you have? Which one/one's would you recommend?
}
} Thanks in advance,
} Peling Melville
}
} --------------------------------------------------------------
} Peling Fong Melville
} Senior Scientific Assistant
} Interdepartmental Facilities
} American Museum of Natural History
} Central Park West at 79th Street
} New York, NY 10024-5192 U.S.A.
} ******************************
} E-mail: peling-at-amnh.org
} Work #: (212) 769-5469
} FAX #: (212) 769-5495

**************************************
* Pete Ainsworth *
* Dept. Geology & Applied Geology *
* Lillybank Gardens *
* University of Glasgow *
* Glasgow G12 8QQ *
* e-mail: ainswort-at-geology.gla.ac.uk *
* Tel : 0141 330 5505 (direct) *
**************************************






From: H. ADAMS :      hadams-at-nmsu.edu
Date: Fri, 18 Oct 1996 08:50:15 -0600 (MDT)
Subject: Re: bacterial biofilms

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On Fri, 18 Oct 1996, Rosemary White wrote:

} Dear all,
}
} A colleague would like to study the development of polysaccharide biofilms
} (secreted by Pseudomonad bacteria) on lettuce leaves. She asked whether
} there were any quick and easy (or, if necessary, long and tricky) ways of
} seeing whether these polysaccharides were present on leaves before and
} after they are turned into salads. My suggestion was to use a simple
} polysaccharide stain on hand sections of leaves, but the film may be too
} thin to distinguish it from the cuticle, even if she compared leaves from
} sterile cultures with field-grown leaves. Any suggestions?
}
} TIA,
}
} Rosemary White
} Department of Ecology and Evolutionary Biology
} Monash University, Clayton, Victoria 3168, Australia
} phone 61-3-9905 5670 email r.g.white-at-sci.monash.edu.au
} fax 61-3-9905 5613 or rgwhite-at-vaxc.cc.monash.edu.au
}
}
Rosemary, in the paper cited below, the author was able to distinguish
between polysaccharides secreted by fungi and bacteria from those
secreted by the plant's root tissues. This paper could be a good starting
point. Citation: R.C.Foster. The Ultrastructure and Histochemistry of the
Rhizosphere. New Phytol. (1981) vol. 89, 263-273.
I hope this helps.
Hank Adams
EML
New Mexico State Univ.




From: Crossman, Harold :      crossman-at-rd.sylvania.com
Date: Fri, 18 Oct 1996 12:02:00 -0400
Subject: Re: BSE detector

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Message-Id: {c=US%a=_%p=SYLVANIA%l=SYLVANIA/OSI/001FFEC2-at-da-exc1.sylvania.com}

Dear Peling Melville and other microscopists,

We, too have a Robinson-type detector, on our field emitter and get
excellent results, especially with pesky samples that have local
charging: multi-phase glasses and ceramics, loose rare-earth phosphor
particles, silicone deposits on metals, etc. It is invaluable in these
cases.

The only "problems" we have are:
Scratching of the aluminum over coat by samples (operator error, not
instrument problem)
The necessity to retract the rod when it is necessary to get closer than
12 mm to the pole piece (it has to go someplace to collect electrons)
"Buck-shot" noise on the CRT when the contrast is very high (just turn
it down)
No opportunity to select/add/subtract, etc. individual channels as in a
plate (nature of the beast)

-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.osram.de
www.siemens.com


Dear Microscopists,

I was wondering about the kinds of BSE detector systems that people have
with their SEM's. What are your opinions? How reliable is the detector
system that you have? Which one/one's would you recommend?

Thanks in advance,
Peling Melville

--------------------------------------------------------------
Peling Fong Melville
Senior Scientific Assistant
Interdepartmental Facilities
American Museum of Natural History
Central Park West at 79th Street
New York, NY 10024-5192 U.S.A.
******************************
E-mail: peling-at-amnh.org
Work #: (212) 769-5469
FAX #: (212) 769-5495






From: Caroline Schooley :      schooley-at-mcn.org
Date: Fri, 18 Oct 1996 08:46:03 -0700
Subject: Re: Project Micro

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MCI/C=US/-at-MHS}
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} The Minnesota Microscopy Society is involved in a preliminary trial of
} the Project Micro Program. One of the experiments calls for examining
} various sands at low magnification (10x-20x). These sands should have
} unique morphological or color properties which could be seen at this
} magnification. If anyone has access to some unique sand we would
} appreciate the help. Please contact me at my EMail address:
}
} RGRappe-at-mmm.com

} Would you be interested in sand collected by my well pump filter? The
} grains are sharp edged and irregular - about 100 microns long. The
} sand is predominantly an even mix of quartz (clear/white) and nearly
} black particles. I forget the composition of the black - I think it
} was comprised of O, Si, Fe, and maybe(?): Mg, Ca, Mn, and or Ti??
} A geologist friend belives it comes from decomposing (poorly
} "sintered") igneous(sp?) rock which was once an undersea deposit of
} volcanic ash.

I hope that everyone agrees that sand samples for MSA's middle school
educational outreach program are an appropriate use of the listserver. I
think that it's a great idea, and I'll be glad to receive labeled samples,
for future distribution. Please send samples first to Rappe - he needs
them now.

Caroline Schooley
Educational Outreach Coordinator, Microscopy Society of America
Box 117 (45301 Caspar Point Road)
Caspar, CA 95420
Phone/FAX: (707)964-9460
Email: schooley-at-mcn.org
Web: http://www.MSA.microscopy.com/ProjectMICRO/Books.html






From: lag-at-pegasus.cc.ucf.edu (Lucille A. Giannuzzi)
Date: Fri, 18 Oct 1996 11:18:03 -0400
Subject: TEM for sale

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Message-Id: {v01540b05ae8d4fa530be-at-[132.170.199.100]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

A Philips EM301 is available for immediate delivery.

The instrument has been on a service contract and has been upgraded with a
goniometer stage and diffusion pump.

For more information, please contact:


*************************************************************************
Lucille A. Giannuzzi, Ph.D.
Assistant Professor

Dept. of Mechanical, Materials, and Aerospace Eng.

University of Central Florida phone (407) 823-5770
PO Box 162450 fax (407) 823-0208
4000 Central Florida Blvd. email lag-at-pegasus.cc.ucf.edu
Orlando, FL 32816-2450 USA
*************************************************************************







From: Tina Geueke :      tina-at-retina.anatomy.upenn.edu
Date: Fri, 18 Oct 1996 14:55:38 -0400 (EDT)
Subject: TEM for sale

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Posted-Date: Fri, 18 Oct 1996 15:10:10 -0400

To whom it may concern:
Please put my e-mail address on your mailing list. Thank you.
Tina Geueke
U of PA






From: slakmon-at-scottscientific.com (P. Slakmon)
Date: Fri, 18 Oct 1996 16:23:53 -0400 (EDT)
Subject: TEM for sale

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I would like to have contact with peolple who are geographically close to
New Orleans who have experience in the installation and configuration of
imaging products such as frame grabber boards, computers, software & CCD
cameras, for light microscopy applications.

_______________________________________________
SCOTT SCIENTIFIC
P.O. Box 66552 Station Cavendish
Montreal, Quebec, H4W 3J6, Canada

Telephone: 514-485-2309 Fax: 514-485-9931
Voice Mail: 514-888-6509

WWW Site: http://www.scottscientific.com

E-Mail: info-at-scottscientific.com
sales-at-scottscientific.com
admin-at-scottscientific.com
links-at-scottscientific.com
_______________________________________________





From: bozzola-at-siu.edu (John J. Bozzola)
Date: Fri, 18 Oct 1996 16:13:39 -0600
Subject: E coli detecting plasts

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A colleague wishes to produce sphaeroplasts (wall-less bacteria) using
lysozyme on cultures of E. coli. Is there a stain capable of
differentiating between normal, walled E. coli cells and sphaeroplasts?
With gram positive cells this is possible with a simple gram stain but this
won't work with E. coli since it is already gram negative. Thank you.

####################################################################
John J. Bozzola, Ph.D., Director
Center for Electron Microscopy
Southern Illinois University
Carbondale, IL 62901-4402
U.S.A.
Phone: 618-453-3730
Fax: 618-453-2665
Email: bozzola-at-siu.edu
Web: http://www.siu.edu/departments/shops/cem.html
####################################################################






From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 18 Oct 96 18:08:07 EDT
Subject: Technical Sales Position

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[[[[[[[[[[[[[[[[[[[[[[[[[[[[[[[[[[[[[[[[[ POSITION OPEN
]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]]

Inside Technical Sales


South Bay Technology, Inc. a small and rapidly expanding laboratory equipment
manufacturer is seeking an innovative, organized and self-motivated individual
for a position in technical sales.

Responsibilities
The successful candidate will report directly to the Vice President of Sales and
Marketing and will be responsible for telephone follow up and qualification of
prospects from leads generated through trade shows, journal advertising,
industry trade groups, etc. The successful candidate will also make regular
contact with existing customers informing them of new product developments and
technical information specific to their application. The position will require
extensive telephone contact and interaction with customers. The position will
also involve responding to quotation requests, taking incoming calls for
information and intitial processing of orders.

Qualifications
Strong interpersonal, organizational and writing skills are absolutely required
as is the ability to work as a member of a team. The candidate should have
strong computer skills -familiarity with ACT!, Microscoft Word, Microsoft Excel,
Microsoft Powerpoint and other software packages is desirable. Scientific
background and/or familiarity with mechanical drawings is a plus.

Salary
Commensurate with experience

Location
South Bay Technology, Inc. is located midway between Los Angeles and San Diego
in the beach front community of San Clemente, CA.


Company Information
You can get additional information on our company via our web site at:
http://www.southbaytech.com.

Please submit your resume to:

Human Resources/Inside Sales Position
South Bay Technology, Inc.
1120 Via Callejon
San Clemente, CA 92673

TEL: 714-492-2600
FAX: 714-492-1499
e-mail: sbt-at-southbaytech.com








From: A. Greene :      ablue-at-mail.io.com
Date: Fri, 18 Oct 1996 21:51:44 -0500 (CDT)
Subject: Film

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Greetings Folks, I have a question regarding Transmission Electron
Microscope Film. A client of mine came up with several old boxes of DuPont
Cromolar Electron Microscope Film. Type EM-7. It was outdated but had been
stored in a freezer. We can find no information as to the speed of the film
or other characteristics. I suppose it can be developed in D-19, like Kodak
film but don't know for sure. Any information about this stuff would be
greatly appreciated. Thanks much for your time.

Alex Greene
Scientific Instrumentation Services, Inc.
Austin, Texas

[INDEPENDENT ELECTRON MICROSCOPE MAINTENANCE]





From: manuela :      manuelap-at-petermac.unimelb.edu.au
Date: Sat, 19 Oct 1996 15:44:10 +1000
Subject: Evans Blue

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Hi,

What does Evens Blue bind to in a cell??

Does anyone know?

Thanks

M.Palatsides.





From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Sun, 20 Oct 1996 10:03:55 +0100 (BST)
Subject: Re: BSE Detectors for SEM

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Dear Peling:

We have the Oxford Instruments TETRA BSe collector and it is fine down
to an accelerarting voltage of ca. 4-5kV. Philips do a "low voltage BSE
collector but I have no idea how well it works. Has any one out there
got or know of a BSE collector which works ar a 1-2 kV or are we up
against the dreaded Laws of Physiscs.

Patrick Echlin
Multi-Imaging Centre CambridgeOn
Thu, 17 Oct 1996, Peling Melville - Interdepartmental Facilities wrote:

} Dear Microscopists,
}
} I was wondering about the kinds of BSE detector systems that people have
} with their SEM's. What are your opinions? How reliable is the detector
} system that you have? Which one/one's would you recommend?
}
} Thanks in advance,
} Peling Melville
}
} --------------------------------------------------------------
} Peling Fong Melville
} Senior Scientific Assistant
} Interdepartmental Facilities
} American Museum of Natural History
} Central Park West at 79th Street
} New York, NY 10024-5192 U.S.A.
} ******************************
} E-mail: peling-at-amnh.org
} Work #: (212) 769-5469
} FAX #: (212) 769-5495
}
}
}





From: Margareta Halin :      Margareta.Halin-at-ah.slu.se,Margareta.Halin-at-medicin.uu.se
Date: Mon, 21 Oct 1996 10:29:39 +0200
Subject: EM-autoradiography

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HI,

I am soon going to start working in a project that involves autoradiography
on the electron microscopical level. There is not very much written about it
(I've read Baker), so I have quite a few questions... One of my questions
is regarding exposure time; I'll be working with a gamma-radiator (In-111).

If you have any idea, please contact me!


My regards,

Margareta Halin
Dept. of anatomy and histology
SLU
Sweden










From: Stefan Mueller-Pfeiffer/Jena/Zeiss/DE :      mueller-pfeiffer-at-zeiss.de
Date: 21 Oct 96 13:43:51
Subject: Zeiss SNOM

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Message-Id: {9610211749.AA3209-at-czjnotes03}
To: spm {spm-at-di.com}

Fellow microscopists,

Carl Zeiss plans the launch of a Scanning Optical Near Field
Microscope (SNOM) in early 1997.

Everybody who is interested in this development may leave his
address on our web-page http://www.zeiss.de/mi/rsm/form.html
and will get further informations as soon as they are released.

Sincerly,

Stefan Mueller-Pfeiffer

-------
Stefan Mller-Pfeiffer, Carl Zeiss Jena GmbH,
Microscope Division

E-Mail: mueller-pfeiffer-at-zeiss.de




From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 21 Oct 1996 09:36:25 EST
Subject: Re: EM-autoradiography

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Microscopy-at-Sparc5.Microscopy.Com

Actually, there's a considerable amount of older information on the
technique itself. M. Salpeter contributed the most to this very
tedious and laborious technique, and has a very good book on the
subject. She also has several chapters in some of the EM techniques
books, such as ADVANCED TECHIQUES IN BIOLOGICAL ELECTRON MICROSCOPY
(J.K. Koehler, ed.).

This should get you started if you REALLY want to do this.



-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Dane Gerneke :      dane-at-uctvms.uct.ac.za
Date: Mon, 21 Oct 1996 15:18:34 -0500
Subject: BS detectors

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-- [ From: Dane Gerneke * EMC.Ver #2.5.02 ] --

Dear Patrick

I have a feeling that what you are looking for is the new CENTARUS BS
detector made by Mike Cowham of KE Developments in Toft. Although I have
not yet been able use one I do understand that it operates well down to
about 600 V - which is good and so is ideal for LV SEM. It also has a
number of other advantages over the Robinson type. One problem I have seen
with the Robinson is having to replace the scintillator after a number of
years. With heat from the beam one finds the scintillator develops cracks
which affects efficiency. A costly exercise.

Mike can be e-mailed at CompuServe: 100610.2353

I look forward to attending your Cryo workshop.

Best regards

Dane Gerneke
E M Unit
University of Cape Town
Tel +27 21 650 2819
Fax +27 21 689 1528
dane-at-uctvms.uct.ac.za




From: A.G.Cullis :      A.G.Cullis-at-sheffield.ac.uk
Date: Mon, 21 Oct 1996 17:59:16 +0100
Subject: MSM X: Conference Announcement

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CONFERENCE ANNOUNCEMENT - Call for Papers

Tenth International Conference on

*************************************************************

MICROSCOPY OF SEMICONDUCTING MATERIALS

*************************************************************

University of Oxford on 7-10 April, 1997
----Abstract deadline: 2 December 1996

Organized on behalf of the Royal Microscopical Society by:
**Prof Anthony G Cullis (a.g.cullis-at-sheffield.ac.uk)
**Dr John L Hutchison (john.hutchison-at-materials.ox.ac.uk)

Co-sponsored by the Institute of Physics (EMAG) and Endorsed by
the Materials Research Society


CENTENARY OF ELECTRON DISCOVERY
------------------------------------------------------------

The conference will feature a special Symposium to celebrate the
centenary of the discovery of the electron. Key presentations will
be given by:
Dr W F Brinkman (Vice-President for Physical Sciences Research,
Bell Labs, Murray Hill)
**The Materials Behind the Telecommunications Revolution
Dr T Matsuo (Managing Director of Semiconductor Equipment Division,
JEOL Ltd, Tokyo)
**The Evolution of Semiconductor E-Beam Lithography and Metrology
Prof K van der Mast (Philips Electron Optics, Eindhoven)
**The Development of Electron-Optical Imaging and Diffraction Systems


MAIN CONFERENCE SCIENTIFIC SESSIONS

These will focus on the most recent advances in the application of
transmission and scanning electron microscopy, X-ray diffraction and
SPM to the study of the structural and electronic properties of
semiconducting materials.

Main topic areas:
- Characterisation of as-grown semiconductors.
- Investigation of lattice defect and impurity behaviour.
- Study of the effects of semiconductor processing treatments.
- Assessment of finished electronic devices.

Special conference sessions:
- Developments in high resolution transmission electron microscopy.
- The nature of epitaxial layers, including quantum well, wire and dot st=
ructures
---- strain relaxation, defect introduction, morphological distortion,
self-organization, luminescence.
- The structure and properties of dislocations and grain boundaries.
- Metal-semiconductor contacts and silicides.
- The effects of processing treatments.
- The exploitation of advanced scanning techniques
---- SEM-EBIC, SEM-CL, etc
---- STM, AFM, BEEM, etc.


INVITED SPEAKERS
-----------------------------

Prof P J Goodhew (University of Liverpool)
**Dislocation Behaviour in Strained Layer Interfaces
Prof R J Hamers (University of Wisconsin-Madison)
**STM Studies of CVD Processes on Si Surfaces
Dr D C Houghton (Canadian National Research Centre, Ottawa)
**Advances in Epitaxial Strained Layer Devices
Dr D E Jesson (Oak Ridge National Laboratory, Tennessee)
**Exploring Instabilities and Metastabilities in Semiconductor Growth
Dr J-L Rouvi=E8re (CEN, Grenoble)
**GaN Growth: Influence of Polarity and Strain
Prof J C H Spence (Arizona State University)
**Dislocation Kink Behaviour in Semiconductors
Prof H P Strunk (University of Erlangen)
**Self-Organization and Defect Mechanisms in Heteroepitaxial Growth
Prof S Takeda (University of Osaka)
**The Structures of Extended Defects in Si and Ge Analysed by HRTEM
Dr R T Tung (Bell Laboratories, Murray Hill)
**Control of Silicide Layers in ULSI Devices: Simple Principles at Work
Dr J Vanhellemont (IMEC, Leuven)
**TEM Studies of Processed Si Device Materials
Dr P R Wilshaw (University of Oxford)
**Developments in SEM:EBIC Studies of Semiconducting Materials

********************************

The Proceedings of the conference will be published. Further details,
including registration and abstract submission information, can be
obtained from: The Administrator, The Royal Microscopical Society,
37/38 St Clements, Oxford OX4 1AJ, UK Tel: +44-(0)1865-248768
Fax: +44-(0)1865-791237 E-mail: meetings-at-rms.org.uk
WWW: http://www.rms.org.uk

********************************




From: Eric :      earosen-at-goodnet.com
Date: Mon, 21 Oct 1996 10:22:48 -0800
Subject: Jobs searches and school majors in biology

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Message-Id: {199610211727.KAA10516-at-goodguy.goodnet.com}
Comments: Authenticated sender is {earosen-at-mail.goodnet.com}

I just have one question to ask you all what the job market is lately
for a biologist with a M.A. or M.S., or B.S. degree?? I am doing
some research into what major I should choose for school and really
like biology but have hard mixed reviews about the job market...

Thanks in Advance.....


\\|//
(o o)
~~~~~~~~~~~~oOOo~(_)~oOOo~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

{ { {This message is made of 100% recycled electrons} } }

Cheers ;o) :o) %o)
Eric
http://www.indirect.com/www/earosen/index.htm




From: Ya Chen :      ychen14-at-facstaff.wisc.edu
Date: Mon, 21 Oct 1996 14:30:17 -0500
Subject: Re: BSE Detectors for SEM

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Hi Peling,

We have an YAG crystal BSE detector modified by Dr. Autrata, Czech
Republic. The BSE detector is installed on a Hitachi S-900 LVSEM and is
capable of detecting 1nm immuno-gold signal. The efficient voltage can be
down to 2.6kV.









From: Woody.N.White-at-mcdermott.com
Date: 10/21/96 3:18 PM
Subject: BS detectors

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What technology is used? Is saturation a problem when using high potentials and
currents? Woody
______________________________ Reply Separator _________________________________


X-Mailer: E-Mail Connection v2.5.03
Content-Transfer-Encoding: 7BIT

-- [ From: Dane Gerneke * EMC.Ver #2.5.02 ] --

Dear Patrick

I have a feeling that what you are looking for is the new CENTARUS BS
detector made by Mike Cowham of KE Developments in Toft.
...snip...
Mike can be e-mailed at CompuServe: 100610.2353

I look forward to attending your Cryo workshop.

Best regards

Dane Gerneke
E M Unit
University of Cape Town
Tel +27 21 650 2819
Fax +27 21 689 1528
dane-at-uctvms.uct.ac.za




From: drstad-at-juno.com (David R Stadden)
Date: Mon, 21 Oct 1996 19:56:47 EDT
Subject: Polaroid Fillm Back Exchange?

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Dear Microscopist Associates,

I've got a surfeit of Polaroid 545 (4"X5") film backs and am looking to
trade one or two for the same size back, but pack film format. I shoot a
lot of Polaroid 553 and could really use another one. Anyone want to do
an even exchange? Please reply to DRStad-at-Juno.com.

Thanks!




From: wise-at-vaxa.cis.uwosh.edu
Date: Mon, 21 Oct 1996 12:48:02 +0000
Subject: A question

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To all,

I gave a seminar last Friday on high resolution SEM of chloroplast
ultrastructure. At the conclusion of my talk I got a question that I have
gotten before: "How do you know that what you are seeing is not an
artifact of fixation?" The answer I always use is: 1) fixation artifacts
are rather apparent so if there was one (membrane blebbing, broken
membranes, etc) I would have discarded the samples. 2) We have been
looking at chloroplasts for over 40 years under a wide variety of fixation
conditions and if the structure as we see it is an artifact, it is an
amazingly consistent one. 3) The structural data are consistent with
biophysical, bioenergetic and biochemical data taken on similar systems.

But this got me thinking so I ask the following question to you all...

Does anyone have an example of an artifact in either SEM or TEM that
seriously mislead the scientific community? I don't wish to get into
criticizing other people's work, but in the 41 years since the invention of
the ultramicrotome (the real start of biological TEM) and the 30 years
since the commercial availability of the SEM, how much impact on scientific
understanding have artifacts had or how much impedance have they provided
to science? What about those of you doing high pressure freezing? What
are the differences you see in samples prepared by HPF vs. standard
chemical fixation?

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-uwosh.edu






From: BOTSS1-at-LURE.LATROBE.EDU.AU
Date: Tue, 22 Oct 1996 13:09:53 +1000
Subject: Mucor for TEM

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hai..I'am having problems with Mucor. Anyone has good protocols and
techniques for preparing Mucor for TEM?
thank you in advance
Widy Sunarpi




From: r.g.white-at-sci.monash.edu.au (Rosemary White)
Date: Tue, 22 Oct 1996 18:11:05 +1200
Subject: Re: A question

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Dear Bob,

In reply to your question:

} Does anyone have an example of an artifact in either SEM or TEM that
} seriously mislead the scientific community? I don't wish to get into
} criticizing other people's work, but in the 41 years since the invention of
} the ultramicrotome (the real start of biological TEM) and the 30 years
} since the commercial availability of the SEM, how much impact on scientific
} understanding have artifacts had or how much impedance have they provided
} to science? What about those of you doing high pressure freezing? What
} are the differences you see in samples prepared by HPF vs. standard
} chemical fixation?

I'm not sure about "seriously" misled the community, but....

First example - Geoff Hyde showed that spore formation in the fungus
Phytophthora was by membrane extension around nuclei, not by fusion of
lined-up membrane vesicles. He saw this with rapid plunge freezing (I
think), not with high pressure freezing, nor with conventional chemical
fixation. People had scratched heads for a long time as to how these
membrane vesicles managed to line up so nicely, and here, finally was the
answer - they didn't! THe next question is - what guides the membranes?
The answer is probably some component of the cytoskeleton - I think he
showed that things are messed up with cytochalasin or oryzalin.

Second example - John Pettit looked at mouse kidney tissue (?organ? - this
is a botanist speaking) in which it was thought that salt secretion was via
vesicles (seen after chemical fixation) that were all lined up, and somehow
they fused with the membrane at a particular spot when secretion was
induced. After slam freezing, instead of lined-up vesicles, in serial
sections he saw long curly membranous tubes shaped a bit like the cord that
connects your telephone ear/mouthpiece to the body of the phone. Active
salt secretion was associated with slight untwisting of the tubes together
with a slight increase in their diameter. Fascinating.

I suspect there are quite a few chemical fixation artefacts like this that
can be avoided with various freezing techniques - as long as you can get
your tissue small enough to avoid ice damage, or can trim it down without
wounding artefacts.....

cheers,

Rosemary White
Department of Ecology and Evolutionary Biology
Monash University, Clayton, Victoria 3168, Australia
phone 61-3-9905 5670 email r.g.white-at-sci.monash.edu.au
fax 61-3-9905 5613 or rgwhite-at-vaxc.cc.monash.edu.au






From: mariani-at-ib.pi.cnr.it
Date: Tue, 22 Oct 1996 12:19:11 -0700
Subject: (no subject)

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Subscribe Microscopy mariani-at-ib.pi.cnr.it




From: lag-at-pegasus.cc.ucf.edu (Lucille A. Giannuzzi)
Date: Tue, 22 Oct 1996 08:26:15 -0400
Subject: Two Positions Available

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Message-Id: {199610221049.FAA02934-at-Sparc5.Microscopy.Com}

Post-Doctoral Position Available

Electron Microscope Engineer Position Available


Two positions are available at the University of Central Florida. The new
Joint Lucent/UCF Materials Characterization Facility currently consists of
a JEOL 2000FX TEM with EDS, Philips EM400T, specimen preparation facility,
JEOL 6400 SEM, JEOL 733 electron microprobe, Riber SIMS, Tandentron RBS. A
Phi Auger, Cameca SIMS, and a Hitachi S-800 SEM will be added within the
year. The facility is located in nearby Research Park.

An electron microscope engineer is desired with an expertise in servicing
and running electron microscopes and related equipment. Responsibilities
will include servicing equipment, training students, and aiding faculty and
staff with analytical procedures.

A Post-Doctoral/Research Associate is desired with an expertise in
(analytical) transmission electron microscopy, and advanced specimen
preparation of materials. Responsibilities will include performing
independent research, training students, and assisting with graduate
student research projects.

UCF is located near Lucent Technologies (formely AT&T), Westinghouse,
Lockheed Martin, NASA Kennedy Space Center, Walt Disney World, Universal
Studies, Harris, and others.

Candidates for either position should submit a resume (please specify the
position your are applying for) and at least three references, to Dr.
Lucille A. Giannuzzi, Mechanical, Materials & Aerospace Engineering,
University of Central Florida, PO Box 162450, 4000 Central Florida Blvd.,
Orlando, FL 32816-2450.

UCF is an affirmative action/equal opportunity employer. As an agency of
the State of Florida, UCF makes all materials available for public review.


*************************************************************************
Lucille A. Giannuzzi, Ph.D.
Assistant Professor

Dept. of Mechanical, Materials, and Aerospace Eng.

University of Central Florida phone (407) 823-5770
PO Box 162450 fax (407) 823-0208
4000 Central Florida Blvd. email lag-at-pegasus.cc.ucf.edu
Orlando, FL 32816-2450 USA
*************************************************************************







From: EmLab :      EMLAB-at-OPUS.MCO.EDU
Date: Tue, 22 Oct 1996 08:54:40 -0400 (EDT)
Subject: Re: Film

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Dear Alex,

Green Box?? If so, I have used this film a long time ago. The emulsion
settings are very similar to 4489 film. Try a few different settings. You
can develope in same D-19 solutions as 4489. I was very mad when this film was
no longer avaiable, it was about $20/box cheaper than kodak film. Oh well.

Best of luck,
Ed Calomeni
Medical College of Ohio
Dept. Pathology
Toledo, OH 43699
emlab-at-opus.mco.edu





From: Donald Lovett :      lovett-at-tcnj.edu
Date: Tue, 22 Oct 1996 10:54:15 -0400 (EDT)
Subject: Re: A question

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I work with crustacean ultrastructure. In a number of species
we have noted structures that look just like the multi-lamellar bodies
associated with surfactant in mammalian lung. Although I (along with
colleagues at other institutions) am convinced that these are not
fixation artifacts, but rather represent real structures, there are many
who do believe that we have fixation artifacts (with justification).

How could we ever prove otherwise?

Additional responses would be welcomed.

Don

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
The College of New Jersey * fax: (609) 771-2674
Trenton, NJ 08650-4700

(* formerly Trenton State College; please note our new name)





From: Michael J. Lyon :      lyonm-at-vax.cs.hscsyr.edu
Date: Tue, 22 Oct 1996 11:05:17 -0400
Subject: diffusible tracer

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I am working with a diffusible tracer, C14-iodoantipyrine and need to
section the tissue. After the experiment the head is frozen in
methylbutane at dry ice temp, freeze dried and then the area of interest
is dissected out. This leave a specimen of } 1 mm sq. I need to then
section the tissue at 20 um. I have seen some older references where
the tissue is placed directly into degassed molten paraffin. Has anyone
had any experience with this technique and willing to pass along their
experiences?

Thanks in advance

Michael




From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Tue, 22 Oct 1996 08:33:12 -0500
Subject: Re: A question

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Message-Id: {199610221325.IAA13159-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"


} Does anyone have an example of an artifact in either SEM or TEM that
} seriously mislead the scientific community?
}
} Bob

This may still be a matter of controversy, but off the top of my
head, I would suggest mesosomes in bacteria. Many people were excited about
them when first seen, but they have come to be regarded as fixation
artifacts, especially from glutaraldehyde.
In SEM, I would regard any measurement made on soft tissue as
artifactual, due to both fixation/dehydration/drying and the physics of how
SEMs work. I'm leery of any measurements, including TEM or LM, of processed
tissues generally.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel *all mail:*
Microscopy
University of Illinois Station A
Rm 74 Bevier Hall PO Box 5037
905 S. Goodwin Ave. Champaign, IL 61825-5037 USA
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu
(217) 355-1143 home

*********** looking for a job again ***********






From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Tue, 22 Oct 1996 09:15:18 EST
Subject: Re: A question

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In my memory, the most widespread instance of a (purported) fixation artifact misleading a
large proportion of the scientific community was that of the proposed
microtrabecular system which was supposed to be common to all
eukaryotic cells. Observations of thick sections of
glutaraldehyde-fixed cultured cells by HV-TEM revealed a very fine
wispy network suspended in the cytosol and numerous functions
(mostly intracellular transport and communication) were attributed to
it. Many imminent scientists were involved with it and many careers
were made studying it. Its validity was always questioned by another
side which (I think) ultimately demonstrated that it was inducible by
chemical fixation.
Anyway, cell biology books of 10-12 years ago covered it, and its
absent from them today. I can probably direct you to specific
literature if you are that interested.


-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Stephan Coetzee :      stephan-at-gecko.biol.wits.ac.za
Date: Tue, 22 Oct 1996 20:21:46 GMT+2
Subject: TEM AISI 431 Jet polishing

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Dear All

Want to jetpolish a AISI 431 Stailess Steel. Using a Fischione twin-
jet. Any sugestions?

Thanks



##
[########]
##
##
##
##
##
Stephan H Coeztee
Electron Microscope Unit
Private Bag 3
Wits
2050
South Africa

Stephan-at-Gecko.biol.WITS.ac.za

Tell: +27 11 716 2419
Fax : +27 11 339 3407




From: Eric Johnston :      ericdj-at-HOME.SEAS.UPENN.EDU
Date: Tue, 22 Oct 1996 14:35:14 -0400
Subject: Macroporous Beads

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Posted-Date: Tue, 22 Oct 1996 14:39:42 -0400 (EDT)
Message-ID: {01BBC026.3DB84F80-at-OAKMONT.SEAS.UPENN.EDU}

Hi

Does anyone know how to reach a company called Microporous Materials =
Limited in Braunston, UK, preferrably a fax number or email address? =
They make a polystyrene bead with porous structure. (I am trying to =
find or develop beads that have a density less than water. No one seems =
to have anything less than 1.02 g/cm^3.)

Thanks

Eric Johnston
Department of Bioengineering
University of Pennsylvania
P: 215-898-1958
F: 215-573-2071
ericdj-at-eniac.seas.upenn.edu




From: kna101-at-utdallas.edu
Date: Tue, 22 Oct 1996 08:58:45 -0500 (CDT)
Subject: Re: Jobs searches and school majors in biology

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Eric,
What kind of job you get depends on what field of biology you want
to study and what
part of the country you are willing to work in AND what kind of salary
you are looking for. There are a fairly good number of jobs in the
medical support field, for instance, but you need to be where there is a
large medical center and you can't be looking for big pay...
I am in a graduate school where many students graduate with a
M.S. in speech pathology or audiology. Here, the field of speech is just
opening up, so there are a fair number of jobs in the area. But this
local area is saturated with audiologists, so to get a good job,
graduates have to be willing to relocate. Also, the audiologists are
talking about switching to a 4 year, post-graduate degree for
certification in the future, so by the time you got to gradute school,
things could be different.
The best way, I think, to approach this is to decide what you
would enjoy doing and how much money you want to start your carrier
making. Don't limit your choices by how long you would be in school. I
did that and here I am, 15 years later, getting the degree to do what I
wanted way back when...

Good luck,
Karen Pawlowski

On Mon, 21 Oct 1996, Eric wrote:

} I just have one question to ask you all what the job market is lately
} for a biologist with a M.A. or M.S., or B.S. degree?? I am doing
} some research into what major I should choose for school and really
} like biology but have hard mixed reviews about the job market...
}
} Thanks in Advance.....
}
}
} \\|//
} (o o)
} ~~~~~~~~~~~~oOOo~(_)~oOOo~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
}
} { { {This message is made of 100% recycled electrons} } }
}
} Cheers ;o) :o) %o)
} Eric
} http://www.indirect.com/www/earosen/index.htm
}




From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Tue, 22 Oct 1996 12:15:06 -0500
Subject: Re: Film

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In message {199610190251.VAA02487-at-tristero.io.com} "A. Greene" writes:
} Greetings Folks, I have a question regarding Transmission Electron
} Microscope Film. A client of mine came up with several old boxes of DuPont
} Cromolar Electron Microscope Film. Type EM-7. It was outdated but had been
} stored in a freezer. We can find no information as to the speed of the film
} or other characteristics. I suppose it can be developed in D-19, like Kodak
} film but don't know for sure. Any information about this stuff would be
} greatly appreciated. Thanks much for your time.
}
} Alex Greene
} Scientific Instrumentation Services, Inc.
} Austin, Texas
}
} [INDEPENDENT ELECTRON MICROSCOPE MAINTENANCE]
}
Hi Alex,

I used to use that film. I developed it in D-19, full strength, for 3.0 minutes,
stop bath 30 sec, Kodak Rapid Fixer for 3.0 minutes, hypoclear 1.0 min, 10
minute minimum water wash. HOWEVER, the 3.0 minute development time only works
if you calibrate your TEM exposure so that you get a good negative at 3.0
minutes. Your TEM must have a way to set a "film speed" parameter, like my
Philips CM12 TEM does. On my former Philips EM300, there was a film sensitivity
control. Most TEM's have a way of measuring electron brightness which is metered
somehow and that meter output can be adjusted by the film sensitivity control.

So if you want to standardize to 3.0 minute development time, then you need to
do a few exposure tests to get a good full toned negative at 3.0 minutes.

Alternatively, if you want to dilute D-19 to 1:1 or 1:2, then perhaps
standardize on 4.0 or 5.0 minute development and calibrate your TEM exposure
accordingly.

Hope this helps!

Gib


Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996
Its over, but not forgotten, and it was a blast!





From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Tue, 22 Oct 1996 15:00:49 +0100 (BST)
Subject: Re: A question

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In reply to the query about chloroplast ultrastructure in the SEM, you
need to distinguish between artefact and damage.
I use the term artefact to describe an altered state ie some which is
there in a natural state but s
"seen" in an altered state. In that respect all EM is an artefact. BUT
what we try to do is to understand the nature of the transformation from
the wet organic reality to the dry inorganic material from which the
image is derived.Sample preparation should be considered as constructive
fossilization.
Damage is where we the operaters muck things up.So the bottom line is:
Artefact is inevitable and needs careful understanding
Damage is inexcsible.

Hope this helps

Patrick Echlin
Cambridge UKOn
Mon, 21 Oct 1996 wise-at-vaxa.cis.uwosh.edu wrote:

} To all,
}
} I gave a seminar last Friday on high resolution SEM of chloroplast
} ultrastructure. At the conclusion of my talk I got a question that I have
} gotten before: "How do you know that what you are seeing is not an
} artifact of fixation?" The answer I always use is: 1) fixation artifacts
} are rather apparent so if there was one (membrane blebbing, broken
} membranes, etc) I would have discarded the samples. 2) We have been
} looking at chloroplasts for over 40 years under a wide variety of fixation
} conditions and if the structure as we see it is an artifact, it is an
} amazingly consistent one. 3) The structural data are consistent with
} biophysical, bioenergetic and biochemical data taken on similar systems.
}
} But this got me thinking so I ask the following question to you all...
}
} Does anyone have an example of an artifact in either SEM or TEM that
} seriously mislead the scientific community? I don't wish to get into
} criticizing other people's work, but in the 41 years since the invention of
} the ultramicrotome (the real start of biological TEM) and the 30 years
} since the commercial availability of the SEM, how much impact on scientific
} understanding have artifacts had or how much impedance have they provided
} to science? What about those of you doing high pressure freezing? What
} are the differences you see in samples prepared by HPF vs. standard
} chemical fixation?
}
} Bob
}
}
} Robert R. Wise, PhD
} Director, UWO Electron Microscope Facility
} Department of Biology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} (414) 424-3404 tel
} (414) 424-1101 fax
} wise-at-uwosh.edu
}
}
}





From: Brad Kort :      bkort1-at-xnet.com
Date: Tue, 22 Oct 1996 16:31:29 -0500
Subject: Particle Atlas on CD-ROM

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Message-ID: {326D3D31.1EFB-at-xnet.com}

I am a student in the Northwestern business school (Kellogg). We are
doing some research for the McCrone Research Institute. Our goal is to
find who could use the following item and what would be a reasonable
price. They have a particle atlas on CD-ROM (2,000 particle images),
with a glossary of terms and techniques.

Any, and all, comments would be very much appreciated. Questions and
comments should be directed to me at:

312/422-2293 (day)
708/361-7613 (evening)
bkort1-at-xnet.com (anytime)

Thanks in advance,
Brad Kort




From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Tue, 22 Oct 1996 09:43:01 -0500
Subject: Re: A question - fixation artifacts

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Message-Id: {v01540b02ae928c6f9c50-at-[128.206.15.189]}
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You are pretty selective on what you call an artifact (blebbing, broken
membrane). One only has to compare an aldehyde fixed mitochondrion with a
quick frozen one. In quick frozen tissues, mitos tend to be smaller, much
more condensed. somebody showed that if you pre-treat the tissue with an
uncoupler of ox-phos, the quick frozen ones look like aldehyde fixed
tissue. In my own work with cholinergic nerve terminals in torpedine rays,
we showed that aldehyde fixed nerve terminals had no vesicles along the
pre-synaptic membrane but quick frozen tissues had a zillion. Aldehyde
fixation caused calcium entry and loss of active zone vesicles. Van
Harreveld showed the classic view of aldehyde fixed brain with no spaces
between anything is an artifact of fixation; quick frozen brain shows much
more extracellular space. Microtubules are lost if one fixes at 4 C but
not if you fix at room temp. Nuclear volume varies significantly depending
on the fix and dehydration protocol. There are lots of other examples. It
is extremely dangerous to assume what one sees (and even prefers visually)
after any type of fixation is totally unaltered.

} To all,
}
} I gave a seminar last Friday on high resolution SEM of chloroplast
} ultrastructure. At the conclusion of my talk I got a question that I have
} gotten before: "How do you know that what you are seeing is not an
} artifact of fixation?" The answer I always use is: 1) fixation artifacts
} are rather apparent so if there was one (membrane blebbing, broken
} membranes, etc) I would have discarded the samples. 2) We have been
} looking at chloroplasts for over 40 years under a wide variety of fixation
} conditions and if the structure as we see it is an artifact, it is an
} amazingly consistent one. 3) The structural data are consistent with
} biophysical, bioenergetic and biochemical data taken on similar systems.
}
} But this got me thinking so I ask the following question to you all...
}
} Does anyone have an example of an artifact in either SEM or TEM that
} seriously mislead the scientific community? I don't wish to get into
} criticizing other people's work, but in the 41 years since the invention of
} the ultramicrotome (the real start of biological TEM) and the 30 years
} since the commercial availability of the SEM, how much impact on scientific
} understanding have artifacts had or how much impedance have they provided
} to science? What about those of you doing high pressure freezing? What
} are the differences you see in samples prepared by HPF vs. standard
} chemical fixation?
}
} Bob
}
}
} Robert R. Wise, PhD
} Director, UWO Electron Microscope Facility
} Department of Biology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} (414) 424-3404 tel
} (414) 424-1101 fax
} wise-at-uwosh.edu

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(573)-882-4712 (voice)
(573)-882-0123 (fax)






From: wise-at-vaxa.cis.uwosh.edu
Date: Mon, 21 Oct 1996 12:48:02 +0000
Subject: A question

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Message-ID: {n1366142431.24925-at-sjdccd.cc.ca.us}
wise-at-vaxa.cis.uwosh.edu
X-Mailer: Mail*Link SMTP-MS 3.0.2

Lots of times this has happened but my life is a bit too busy to go into much
detail.
Look at the mesosomes in bacteria, there were actually fixation artifact,
found when free fracture was done.
The pore complex in basidiomycetes and ascomycetes in fungi.
and there are plenty more.
The cell membrane in bacteria often is artifactual shown true by free fracture
where there is no fixation.

Good luck, its actually fun to make a list and look through the literature for
them as I have my students do.

Judy M.

Judy Murphy, PhD
Microscopy Technology Center
San Joaquin Delta College
5151 Pacific Ave
Stockton, CA 95207

Phone: 209/474-5284
FAX: 209/474-5600
e-mail: murphy-at-sjdccd.cc.ca.us
program web page: http://www.sjdccd.cc.ca.us/ElectMicro/sjdc.html

_______________________________________________________________________________

To all,

I gave a seminar last Friday on high resolution SEM of chloroplast
ultrastructure. At the conclusion of my talk I got a question that I have
gotten before: "How do you know that what you are seeing is not an
artifact of fixation?" The answer I always use is: 1) fixation artifacts
are rather apparent so if there was one (membrane blebbing, broken
membranes, etc) I would have discarded the samples. 2) We have been
looking at chloroplasts for over 40 years under a wide variety of fixation
conditions and if the structure as we see it is an artifact, it is an
amazingly consistent one. 3) The structural data are consistent with
biophysical, bioenergetic and biochemical data taken on similar systems.

But this got me thinking so I ask the following question to you all...

Does anyone have an example of an artifact in either SEM or TEM that
seriously mislead the scientific community? I don't wish to get into
criticizing other people's work, but in the 41 years since the invention of
the ultramicrotome (the real start of biological TEM) and the 30 years
since the commercial availability of the SEM, how much impact on scientific
understanding have artifacts had or how much impedance have they provided
to science? What about those of you doing high pressure freezing? What
are the differences you see in samples prepared by HPF vs. standard
chemical fixation?

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-uwosh.edu



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From: Jim Darley :      p&s-at-ultra.net.au
Date: Wed, 23 Oct 1996 14:41:02 +1000
Subject: Re: A question

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Message-Id: {1.5.4.32.19961023044102.00671544-at-mailhost.ultra.net.au}
X-Sender: pns-at-mailhost.ultra.net.au
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Mime-Version: 1.0
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At 10:54 22/10/96 -0400, you wrote:
}
} I work with crustacean ultrastructure. In a number of species
} we have noted structures that look just like the multi-lamellar bodies
} associated with surfactant in mammalian lung. Although I (along with
} colleagues at other institutions) am convinced that these are not
} fixation artifacts, but rather represent real structures, there are many
} who do believe that we have fixation artifacts (with justification).
}
} How could we ever prove otherwise?
}
} Additional responses would be welcomed.
}
} Don
}
} ______________________________________________________________________
} Donald L. Lovett e-mail: lovett-at-tcnj.edu
} Assoc. Professor, Dept. of Biology voice: (609) 771-2876
} The College of New Jersey * fax: (609) 771-2674
} Trenton, NJ 08650-4700
}
} (* formerly Trenton State College; please note our new name)
************************************************
Don have you tried staining GA only fixed tissue with Sudan Black B or
another lipid stain? Over 30 years ago it was shown that poorly fixed lipid
droplets can form myelin-like, concentric artificial membranes.
Alternatively you could treat the sections with lipase and digest any
lipids. I rather expect that you are seeing such lipid droplets.
However, if those structures are "real", chances are that they are lipid
based anyway.
Regards
Jim Darley

Probing & Structure
} (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia
}
} Phone +61 77 740 370 Fax: +61 77 892 313
} A great microscopy site http://www.ultra.net.au/~pns/





From: azriel gorski :      azrielg-at-cc.huji.ac.il
Date: Wed, 23 Oct 1996 10:01:32 +0200 (GMT+0200)
Subject: Re: Particle Atlas on CD-ROM

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I currently use it, and find it worth every bit worth the price it was.

Hope that helps.

Shalom from Jerusalem,
Azriel Gorski

+++++++++++++++++++++++++++++++++++++++++++++++
Azriel Gorski, Head
Optical Microscopy Laboratory
Division of Identification and Forensic Science
Israel National Police
91906 Jerusalem



On Tue, 22 Oct 1996, Brad Kort wrote:

} I am a student in the Northwestern business school (Kellogg). We are
} doing some research for the McCrone Research Institute. Our goal is to
} find who could use the following item and what would be a reasonable
} price. They have a particle atlas on CD-ROM (2,000 particle images),
} with a glossary of terms and techniques.
}
} Any, and all, comments would be very much appreciated. Questions and
} comments should be directed to me at:
}
} 312/422-2293 (day)
} 708/361-7613 (evening)
} bkort1-at-xnet.com (anytime)
}
} Thanks in advance,
} Brad Kort
}





From: Gary Dietrich Chinga :      garyc-at-stud.ntnu.no
Date: Wed, 23 Oct 1996 10:54:01 +0100 (MET)
Subject: Scanning and DIP

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Posted-Date: Wed, 23 Oct 1996 10:54:01 +0100 (MET)


Hi !

We are trying to find out how to connect a Mac computer to a Scanning EM
Jeol JSM 5310 microscope.

What equipment do we have to purchase (CCD camera, +++?)

Does anyone have some experience with this kind of equipment?

GCH...




From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Wed, 23 Oct 1996 10:46:12 +0100 (BST)
Subject: EDXA of Ar

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Dear Fellow Sufferers,
I am trying to analyse sputtered films by TEM/EDXA.
These contain a significant amount of Ar, but I don't have a
suitable standard (isn't that strange!). I can calculate a
theoretical k factor which will give a reasonable estimate,
but that still leaves me scratching around for a decent peak
profile. Has anyone any solutions to this problem?
Regards,
Eric


Dr Eric Lachowski
University of Aberdeen
Department of Chemistry
tel +44 1224 272934
fax +44 1224 272921
e.lachowski-at-abdn.ac.uk





From: Oldrich Benada :      benada-at-sun1.biomed.cas.cz
Date: Wed, 23 Oct 1996 10:22:21 +0100
Subject: Alcian Blue

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Message-Id: {9610230822.AA28654-at-sun1.biomed.cas.cz}
Comments: Authenticated sender is {benada-at-sun1.biomed.cas.cz}

Dear colleagues,
I should like to use Alcian blue for fixation of envelope of
bacteria (A procedure which was reviewed by T.A. Fassel et al.,
1992). I have problems with dissolving this dye. Although I have
purified it according to M.A. Hayat (1989), I can'n prepared 0.5%
stock solution without any precipitate. I an using Alcian blue 8 GS
(8 GX) for Microscopy FLUKA, Cat.No.05500.

Could anyone give me some advice?

Thanking you in advance
Your sincerely
O. Benada
+---------------------------------------------------------------+
Dr. Oldrich Benada
Acad. Sci. CR Phone: +42-2-4752399
Institute of Microbiology Fax: +42-2-4715743
Electron Microscopy Group E-mail: benada-at-biomed.cas.cz
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+---------------------------------------------------------------+




From: csbeneas :      csbeneas-at-wiccmail.weizmann.ac.il (by way of Nestor J.
Date: Wed, 23 Oct 1996 07:30:32 -0500
Subject: artifacts

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Zaluzec)

Hi, everybody,
I agree with Philip Oshel (message from 22.10.1996) that we cannot belive to
the measurments of the fixed and especialy dehydrated tissues.
Do somebody have an opinion about all cryo methods do they affect the size of
cells and organels and how significant these changes are?






From: Laurence Tetley :      gbza40-at-udcf.gla.ac.uk
Date: Wed, 23 Oct 1996 09:21:34 +0100 (BST)
Subject: Artifacts thread

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The current expanding discussion of examples of artifacts in EM hasn't
mentioned that there's a book on the subject published in 1988 which could
be usefully consulted called , not surprisingly,

Artifacts inBiological Electron Microscopy eds Richard Creng / Karen Klomparens
Plenum, ISBN 0-306-42863-6


it isn't concerned too much with what's recently been alluded to ie. results
from cryotechniques being probably the strongest evidence for determining
what are artifacts in chemically prepared specimens - but it's worth a look
for the uninitiated dealing with conventionally prepared specimens. It goes
into some detail on a range of artifacts in TEM, SEM and allied techniques
including those generated by improper use of the instruments themselves.

Laurence Tetley
Dr Laurence Tetley
IBLS EM Centre
Joseph Black Building
University of Glasgow
Glasgow G12 8QQ

email gbza40-at-udcf.gla.ac.uk
tel. 0141 330 4431
fax 0141 307 8016





From: kris-at-elod.vein.hu (Kris Kovacs)
Date: Wed, 23 Oct 1996 14:36:16 +0100
Subject: Particle Atlas

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Dear Fellow Microscopists,

Some of you know more about the Particle Atlas CD than I do. Where is it
available? Who does it? How much it costs? The last Particle Atlas I saw
(and have) was published by Ann Arbor Science in 1973 (Second Edition).
There should be something fresher out there...
Thanks for any information I get.

Kris
==================================================================



*************************************************************************
Dr. Kristof KOVACS
Associate Professor
University of Veszprem, Central Laboratory
P.O.Box 158, Veszprem, HUNGARY
H-8201
Phone: +36-(88)-421-684
*************************************************************************





From: wise-at-vaxa.cis.uwosh.edu
Date: Wed, 23 Oct 1996 10:47:07 +0000
Subject: Question for vendors only

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Vendors,

I am looking for price and availability information on thermal
paper for Mitsubishi video copy processor model K65H. Please repond to me
and not the entire net.

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-uwosh.edu






From: John Gabrovsek :      gabrovj-at-cesmtp.ccf.org
Date: Wed, 23 Oct 1996 09:26:42 -0400
Subject: Membrane labeling

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Message-Id: {s26de680.080-at-cesmtp.ccf.org}
X-Mailer: Novell GroupWise 4.1

Hi everybody,
I need some advice.
We would like to check for the proportion of vesicles which are outside
out as opposed to inside out in liver plasma membranes.
Does anyone know of, or has used, a cytochemical marking for TEM to
selectively label:
a) only the extracellular domain or
b) only the intracellular domain of a typical common plasma membrane
protein.
In particular, has anyone used colloidal gold-tagged lectins to bind
membrane glycoproteins? If so, do you have a protocol?
Any advice will be appreciated including suggested readings.
TIA
Regards,
John Gabrovsek
CCF Cleveland, Ohio







From: LLOYD WILLIAMS :      HOSTA.williams-at-genectr.hunter.cuny.edu
Date: Wed, 23 Oct 1996 14:00:00 -0500 (EST)
Subject: COMPIX vs Metamorph

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We are currently upgrading our Image analysis software and are
considering purchasing either Universal Imaging's Metamorph or
COMPIX's C*Imaging product. And I was wondering wether any listserv
subscribers have any experience with either product that might help us
giude our decision.
Thanks in advance
Lloyd Williams
Manager of Bio Imaging Facility
Center for Study of Gene Structure and Function
Hunter College
e-mail williams-at-genectr.hunter.cuny.edu




From: Henrik Kaker :      Henrik.Kaker-at-guest.arnes.si
Date: Wed, 23 Oct 1996 20:49:29 +0100
Subject: Re: Particle Atlas

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Cris,

You may found more about Particle Atlas on Web site of McCrone Research
Institute. URL address is in the my microscopy vendors database.

Henrik
--
Henrik Kaker
SEM-EDS Laboratory, Metal Ravne d.o.o., Slovenia,
Tel: +386 602 21 131 Fax: +386 602 20 436
Henrik.Kaker-at-guest.arnes.si,
http://www2.arnes.si/guest/sgszmera1/index.html
Microscopy Vendors Database:
http://www2.arnes.si/guest/sgszmera1/vendors.html




From: Jeffrey.Shield-at-mse.utah.edu (Jeff Shield)
Date: Wed, 23 Oct 1996 13:39:01 -0600 (MDT)
Subject: SEM: immobile stages

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Greetings,

The z-control in our Cambridge S240 refuses to budge. I was wondering if
anyone had any helpful hints before we tackle it. Any help is greatly
appreciated, since getting phone support has proven difficult. Thanks.

Jeff

------------------------------------------------------------------
-- Jeff Shield --
-- Assistant Professor --
-- Department of Materials Science and Engineering --
-- University of Utah --
-- Salt Lake City, Utah 84112 --
-- 801/581-3179 --
------------------------------------------------------------------






From: Woody.N.White-at-mcdermott.com
Date: 10/22/96 4:31 PM
Subject: Particle Atlas on CD-ROM

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You might elaborate a bit... Particles of what? Mode of imaging?
Other data? Woody


______________________________ Reply Separator _________________________________


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I am a student in the Northwestern business school (Kellogg). We are
doing some research for the McCrone Research Institute. Our goal is to
find who could use the following item and what would be a reasonable
price. They have a particle atlas on CD-ROM (2,000 particle images),
with a glossary of terms and techniques.

Any, and all, comments would be very much appreciated. Questions and
comments should be directed to me at:

312/422-2293 (day)
708/361-7613 (evening)
bkort1-at-xnet.com (anytime)

Thanks in advance,
Brad Kort




From: Adam Rupert :      arupert-at-inet.guthrie.org
Date: Tue, 22 Oct 1996 22:07:39 -0400
Subject: Inquiry of electron microscopy

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X-Authentication-Warning: gw: Host [10.17.1.22] claimed to be
Message-Id: {326D7DEB.66AA-at-inet.guthrie.org}

To Whom It May Concern:
I am a recent graduate of Mansfield University (B.S. in Biology with a Chemistry minor)
and a current student at Robert Packer Hospital's School of Medical Technology.
At Mansfield Uni. I conducted undergraduate research on a malignant, murine macrophage
cell line --- P388D1 (ATTC). I experimented with carbonyl iron uptake and cell cycle
correlations. The only thing that I was unable to find with this particular cell line in my
literature search was electron microscopy. If I had some background of its cellular and surface
components, I would better understand the cell response from my research. At the current moment
I intend to continue the research here at RPH in 1997 with a stronger emphasis on membrane
receptors with the use of Flowcytometry.
It would be greatly appreciated if I could find this information of electron microscopy
of this cell line through your organization or through your reference to another. Thank you for
your time.

Sincerely,

Tammy M. Walker

I can be contacted at: twalker-at-inet.guthrie.org




From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: 10/22/96 4:31 PM
Subject: Particle Atlas on CD-ROM

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Brad;

I believe that the particle atlas on CD-ROM would be very useful to people (like
myself) in the medical device, pharmaceutical, and biotech fields. I routinely
have to isolate and identify particle contaminants in and on such products,
using various microscopic and spectroscopic techniques. The price should be
comparable to (or hopefully less than) what we would pay for the book version.

-Bob
**********************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA 91711
USA
ph: (909)399-1311
email: Bob_Citron-at-cc.chiron.com
**********************************

______________________________ Reply Separator _________________________________


I am a student in the Northwestern business school (Kellogg). We are
doing some research for the McCrone Research Institute. Our goal is to
find who could use the following item and what would be a reasonable
price. They have a particle atlas on CD-ROM (2,000 particle images),
with a glossary of terms and techniques.

Any, and all, comments would be very much appreciated. Questions and
comments should be directed to me at:

312/422-2293 (day)
708/361-7613 (evening)
bkort1-at-xnet.com (anytime)

Thanks in advance,
Brad Kort




From: tmitche-at-mst.lanl.gov (Terry Mitchell)
Date: Wed, 23 Oct 1996 09:28:20 -0700
Subject: EM staff position

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Dear colleagues,

Los Alamos will shortly be advertising for a technical staff member
in the Electron Microscopy Facility. Here is the job description:

Summary:

Work in the Center for Materials Science, Materials Science and Technology
Division at Los Alamos National Laboratory supporting a variety of basic
energy sciences and other DOE programs. Provide expertise in electron
microscopy and be part of a team that operates the CMS Electron Microscopy
Facility. In particular take the lead in operating and maintaining a VG
HB601UX field-emission transmission elecron microscope, a JEOL 3000F
field-emission high resolution transmission electron microscope and a
Philips CM30 analytical transmission electron microscope, along with
associated parallel electron energy loss systems, x-ray energy dispersive
systems and ccd cameras. Be involved in all aspects of thin film sample
preparation. Teach and assist other employees and visitors in the operation
of the microscopes. Be involved in research that uses the electron
microscopes.

Required Skills, Knowledge, Abilities:

The successful candidate will possess a strong background in electron
microscopy with an emphasis on applications to materials science.
Demonstrated skills in STEM, TEM, HREM, PEELS, EDS and associated computer
programs. Research experience in electron microscopy applications in
materials science. Effective written and oral communication skills, as
evidenced by publications and presentations in the electron microscopy
field. Must be able to perform successfully in a team environment.

Desired Skills, Knowledge, Abilities:

Experience in operating an electron micoscopy facility involving large
numbers of users.

Education, Training, or Licensing:

Ph.D. in materials science, physical sciences, or equivalent combination of
education and experience. Minimum of 10 years experience in operating
transmission electron microscopes.

Qualified people can indicate their interest by e-mailing me and attaching
their cv.

Terry Mitchell

**********************************
* Terence E. Mitchell
* Center for Materials Science
* Mail Stop K765
* Los Alamos National Laboratory
* Los Alamos, NM 87545
* Tel: 505-667-0938
* Fax: 505-665-2992
* E-mail: temitchell-at-lanl.gov
**********************************






From: mgb-at-nucleus.ansto.gov.au (Mark Blackford)
Date: Thu, 24 Oct 1996 08:55:34 +1100
Subject: Re: EDXA of Ar

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Message-Id: {199610232254.IAA00830-at-nucleus.ansto.gov.au}
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Eric,

I produced a sample for Ar profiles by gluing a graphite disc to a copper
slot grid then dimpling and ion beam thinning it. I found that sufficient
Ar was implanted into the graphite to allow suitable spectra from which
profiles could be made.

} Dear Fellow Sufferers,
} I am trying to analyse sputtered films by TEM/EDXA.
} These contain a significant amount of Ar, but I don't have a
} suitable standard (isn't that strange!). I can calculate a
} theoretical k factor which will give a reasonable estimate,
} but that still leaves me scratching around for a decent peak
} profile. Has anyone any solutions to this problem?
} Regards,
} Eric
}
}
} Dr Eric Lachowski
} University of Aberdeen
} Department of Chemistry
} tel +44 1224 272934
} fax +44 1224 272921
} e.lachowski-at-abdn.ac.uk

Mark Blackford
TEM Group
Materials Division, Ansto
PMB 1,
Menai, N.S.W.
Australia
2234
Phone (02) 9717 3027
Fax (02) 9543 7179






From: Glen MacDonald :      glenmac-at-franklin01.u.washington.edu
Date: Wed, 23 Oct 1996 10:37:52 -0700
Subject: Re: Scanning and DIP

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Microscopy-at-Sparc5.Microscopy.Com T

Chinga) wrote:


Gary,
No camera is necessary. 1. You can use a Mac based sytem to control the SEM (4
Pi Analysis makes one; www.4pi.com) or a PC based system. these will cost
US$8,000 and up. 2. some kind soul who saved it might send you a recent message
outlining how to build your own Mac controller for under $1,000. 3. Run a coax
cable from the framegrabber in your Mac to the video out BNC on your SEM. On
our JEOL 6300, the video out is behind the kick panel under the console. This
last is the simplest method, but you are limited to the resolution of the signal
to the video monitor. However, I found that it is quite sufficient for many
purposes.

} We are trying to find out how to connect a Mac computer to a Scannig EM
} Jeol JSM 5310 microscope.
Regards,
Glen MacDonald
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu





From: Michael OKeefe :      Michael_OKeefe-at-macmail.lbl.gov
Date: 23 Oct 1996 11:24:58 -0700
Subject: Re: Particle Atlas

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Message-ID: {n1366052238.17577-at-macmail.lbl.gov}
"Microscopy-at-Sparc5.Microscopy.Co" {Microscopy-at-Sparc5.Microscopy.Com}
Cc: "Jan-Olle Malm" {jan-olle.malm-at-oorg2.lth.se}
X-Mailer: Mail*Link SMTP/QM 3.0.0

Reply to: RE} Particle Atlas

} Date: 10/23/96 8:19 AM
} From: Kris Kovacs
} Dear Fellow Microscopists,

} Some of you know more about the Particle Atlas CD than I do.
} Where is it available? Who does it? How much it costs?
} The last Particle Atlas I saw (and have) was published by
} Ann Arbor Science in 1973 (Second Edition).
} There should be something fresher out there...
} Thanks for any information I get.

} Kris
} =================================================================
} Dr. Kristof KOVACS
} Associate Professor
} University of Veszprem, Central Laboratory
} P.O.Box 158, Veszprem, HUNGARY
} H-8201
} Phone: +36-(88)-421-684
} *********************************************************

Also, please --
What sorts of particles?
What sizes?
What microscope(s)?

:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:
Michael A. O'Keefe, Deputy Head
National Center for Electron Microscopy
Lawrence Berkeley National Laboratory
University of California
Berkeley, California 94720
tel: (510) 486-4610
fax: (510) 486-5888
email: maok-at-lbl.gov
http://ncem.lbl.gov/
:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:.:







From: John Turek :      jjt-at-vet.purdue.edu
Date: Wed, 23 Oct 1996 12:19:09 -0500
Subject: Indium-111 autoradiograghy

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Message-Id: {1.5.4.32.19961023171909.006cddf4-at-vet.purdue.edu}
X-Sender: jjt-at-vet.purdue.edu
X-Mailer: Windows Eudora Light Version 1.5.4 (32)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

HI,

I am soon going to start working in a project that involves autoradiography
on the electron microscopical level. There is not very much written about it
(I've read Baker), so I have quite a few questions... One of my questions
is regarding exposure time; I'll be working with a gamma-radiator (In-111).

If you have any idea, please contact me!


My regards,

Margareta Halin
Dept. of anatomy and histology
SLU
Sweden




We have performed autoradiography using IN-111 on one micron sections of
epoxy embedded tissues. The half-life of IN-111 was so short that within 5
days after collecting tissues from animals injected with an IN-111 compound,
there was not sufficient radioactivity left to expose an emulsion. The trick
is to get the material embedded, sectioned, and dipped in emulsion as soon
as possible. I would suggest a rapid embedding procedure (microwave) so
that you get the emulsion on the sections within 24 hours. An exposure time
of 3-5 days should be sufficient.
John J. Turek, Ph.D.
Purdue University
Dept. of Basic Medical Sciences
Core Laboratory for Image Analysis
and Multidimensional Applications (CRISTAL)
phone: 317-494-5854
fax: 317-494-0781





From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Thu, 24 Oct 1996 10:52:55 +1000 (EST)
Subject: Re: Artefacts in Biological Microscopy

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The topic of artefact in microscopy goes back at least to Robert Hookes
"Microscopia" in which he warns microscopists against "mistaking the shadow
for the substance" Pretty apt even today.

I think the only honest way is to take the approach suggested by Edgar
Mercer. All biological electron microscopy IS AN ARTEFACT. Sure, it starts
with a real thing and is based on a real thing but it is not the same as the
real thing. Our only excuse is that we understand the changes we have
induced and that the artefact is repeatable. There are degrees of change,
too. A gold coated fly in an SEM has been altered less than a sectioned
chloroplast.

It is instructive to read the challenges to electron microscopy thrown out
by the gadfly Harold Hillman. See Hillman and Sartory, Perception, 1977 Vol
6, pp 667-673 and anything else Harold has challenged. Get your students
to read them and answer the challenges. THAT will teach them some microscopy!

Mel Dickson
EM Unit, UNSW





From: azriel gorski :      azrielg-at-cc.huji.ac.il
Date: Wed, 23 Oct 1996 20:44:25 +0200 (GMT+0200)
Subject: Re: Particle Atlas

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The Particle Atlas Electronic Edition was put out by MicroDataware which
is no longer in business. It is a very nice electronic, computer
searchable copy of the 6 Volumns of the Old Particle Edition. The search
ability is NICE!!!!!

The cost was about US$ 1500.00.

As I stated MicroDataware, and this is a shame, is no more. From the note
I saw here, it seems that the McCrone Research Institute is looking at
re-issue of it in some form.

If they do, I wish them luck. But specifically I wish them sales.

Shalom from Jerusalem,
Azriel

On Wed, 23 Oct 1996, Kris Kovacs wrote:

} Dear Fellow Microscopists,
}
} Some of you know more about the Particle Atlas CD than I do. Where is it
} available? Who does it? How much it costs? The last Particle Atlas I saw
} (and have) was published by Ann Arbor Science in 1973 (Second Edition).
} There should be something fresher out there...
} Thanks for any information I get.
}
} Kris
} ==================================================================
}
}
}
} *************************************************************************
} Dr. Kristof KOVACS
} Associate Professor
} University of Veszprem, Central Laboratory
} P.O.Box 158, Veszprem, HUNGARY
} H-8201
} Phone: +36-(88)-421-684
} *************************************************************************
}





From: Mary Mager :      mager-at-unixg.ubc.ca
Date: Wed, 23 Oct 1996 19:50:24 -0700 (PDT)
Subject: Re: COMPIX vs Metamorph

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} We are currently upgrading our Image analysis software and are
} considering purchasing either Universal Imaging's Metamorph or
} COMPIX's C*Imaging product.
Dear Lloyd,
We have been using the Compix product for two years now. The system is used
almost all day, every day for grain size measurements on metal. It is very
robust, easy to use and modify for your particular use. i have no knowledge
of the Universal product.
Regards,
Mary
Mary Mager
Electron Microscopist
Metals and Materials Eng., UBC
6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel:604-822-5648, fax:604-822-3619
e-mail: mager-at-unixg.ubc.ca





From: r.g.white-at-sci.monash.edu.au (Rosemary White)
Date: Thu, 24 Oct 1996 14:36:40 +1200
Subject: Re: Indium-111 autoradiograghy

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Dear Margareta,

This isn't my area, but I thought people had switched to various methods
where the target is visualised with gold probes, because film exposure
times were so long. Anyway, there is reference to several papers (almost
all in animal cells) using radiolabelled probes in Hall and Hawes (1991) -
Electron microscopy of Plant cells - pp. 221-222, that may be useful.

cheers,

Rosemary White
Department of Ecology and Evolutionary Biology
Monash University, Clayton, Victoria 3168, Australia
phone 61-3-9905 5670 email r.g.white-at-sci.monash.edu.au
fax 61-3-9905 5613 or rgwhite-at-vaxc.cc.monash.edu.au






From: DVCCO-at-aol.com
Date: Thu, 24 Oct 1996 04:11:53 -0400
Subject: Re: Particle Atlas on CD-ROM

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Dear Azriel,

If you are in need of 10 bit/ or very high signal to noise } 62dB monochrome
real time CCD cameras, digital or analog, for your microscope systems feel
free to look at our very informative web site / Q &A section, and rundown on
how to choose a CCD camera, and what to look for.

http://www.edt.com/dvc/dvc.html

We supply complete PCI bus imaging systems/ frame grabbers etc., from many
different manufacturers in US and Canada. Software also.

Regards,

Richard Klotsche
DVC Company
619-444-8300
619-444-8321-fax




From: clsmteam-at-imiucca.csi.unimi.it
Date: Thu, 24 Oct 1996 10:02:44 GMT
Subject: TEM immunohistochemistry

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To all
Is there anyone who has experience of TEM
immunohistochemistry on human skin?
Can he send us the methods used and is observations on
cryofixation, cryoprotection and so on?

Thanks you.


Prof. Paolo Castano
Istitute of Anatomy
Via Mangiagalli 31, 20133 Milan
Italy
E-mail clsmteam-at-imiucca.csi.unimi.it





From: James Lewis :      jlewis-at-jesus.ox.ac.uk
Date: Thu, 24 Oct 1996 11:11:23 +0100 (BST)
Subject: Please delete

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} Please can my address be deleted from the microscopy mailing list.

Many thanks

James Lewis




From: es0mhs-at-environment.sunderland.ac.uk (HASWELL Malcolm)
Date: Thu, 24 Oct 1996 10:58:35 +0100
Subject: Osmium waste

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What does everyone else do with their osmium tetroxide waste?

We only use a few grams a year so it is allowed to accumulate in a waste
bottle of osmium 'black' until it annoys me and goes to a waste disposal
company. I have worked in a lab where it was re-cycled into low grade osmium
tetroxide but it was time consuming and the end product could not be used
for demanding work.

Are there any commercial companies which re-process the waste or is this
un-economical because of the relatively low value of osmium, at present, and
the potential mix of buffer vehicle components? The reason I ask is that I
used to hear rumours about chemical companies recovering osmium but I have
never actually traced one.

This question is more about the environment than economics and I know the
same question can be asked of other elements such as silver in photography.

Incidentally, I would like to thank everyone for their suggestions about our
problem with a molybdenum artefact peak in x-ray microanalysis. We have
greatly reduced the problem by having our service engineer paint the exposed
edges of the objective aperture rod with carbon DAG.

Malcolm Haswell
E.M Unit
University of Sunderland
UK





From: Loudy, David E. :      davidloudy-at-mmd.com
Date: Thu, 24 Oct 1996 08:55:00 -0500
Subject: Compix vs Metamorph

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Message-Id: {199610241256.AA24366-at-gate.mmd.com}

Our Pathology /Imaging group has been using the C-imaging system for 3 years and find it very useful for quantitating samples ranging from
immunocytochemistry slides to autoradiograms. I have not seen an imaging product that can perform 1 Mb grey scale operations as fast as
Simple with the 1280 Matrox boards (1000 mips, In was told). Software is very user friendly, and a win95 version is due out soon. I
strongly recommend. If you would like more info, you can e-mail directly at davidloudy-at-mmd.com.




From: Lesley S. Smith :      lesleys-at-pobox.upenn.edu
Date: Thu, 24 Oct 1996 09:13:31 -0400 (EDT)
Subject: Epon Blocks

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A friend who does SEM mostly but is doing more TEM asked me the
other day why her epon blocks were sticking in the mold. She said that
they were cutting them out with razor blades but it was getting costly. I
also use razor blades and have cut myself numerous times when the blade
stuck. I even ended up with stitches on two fingers from one of these
incidents. I have heard of using liquid nitrogen to remove blocks but as
I've never seen it done, I have no idea how this works. But I thought
there must be a better way to remove blocks. Does anyone have any easier
and safer methods for removing epon blocks from their molds?

Thanks in advance!!

Lesley Bechtold





From: tphillips-at-biosci.mbp.missouri.edu (Tom Phillips)
Date: Thu, 24 Oct 1996 09:55:22 -0500
Subject: Re: Membrane labeling

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Message-Id: {v01540b00ae9532ba709f-at-[128.206.15.189]}
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Dozens, if not hundreds, of papers describing collodial gold lectin
labeling of the extracellular membrane are out there. Among them is Frisch
& Phillips 1990 Lectin binding patterns to plasmalemmal glycoconjugates of
goblet cells undergoing differentiation in vitro. J. Electron Microscopy
Technique 16:25-36. WGA would be an obvious starting place. Another
common approach is to use cationized ferritin to non-specifically label the
extracellular face of the plasmalemma. Cationized gold is also now
available.



} Hi everybody,
} I need some advice.
} We would like to check for the proportion of vesicles which are outside
} out as opposed to inside out in liver plasma membranes.
} Does anyone know of, or has used, a cytochemical marking for TEM to
} selectively label:
} a) only the extracellular domain or
} b) only the intracellular domain of a typical common plasma membrane
} protein.
} In particular, has anyone used colloidal gold-tagged lectins to bind
} membrane glycoproteins? If so, do you have a protocol?
} Any advice will be appreciated including suggested readings.
} TIA
} Regards,
} John Gabrovsek
} CCF Cleveland, Ohio

Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(573)-882-4712 (voice)
(573)-882-0123 (fax)






From: Mark Wall :      Mark.Wall-at-quickmail.llnl.gov
Date: 24 Oct 1996 08:07:49 -0800
Subject: Mark Wall

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Message-ID: {n1365977979.82967-at-quickmail.llnl.gov}

Does anyone know of any commercial services providing sectioning and/or
cryosectioning of bio materials near me in northern Calif.? Please respond by
E-mail.

Thanks,

Mark A. Wall
Lawrence Livermore National Lab
Livermore, CA 94550
510-423-7162





From: Linda Fox :      lfox1-at-wpo.it.luc.edu
Date: Thu, 24 Oct 1996 10:10:41 -0500
Subject: CPD free floating section

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Message-Id: {s26f400a.062-at-wpo.it.luc.edu}
X-Mailer: Novell GroupWise 4.1

Greetings Friends, Can anyone help with a proceedure for CPD a
50um free floating section? It is brain tissue that has been fixed,
cryosectioned and returned to buffer. All wizardry is welcome at
this point !! Has anyone used Peldri II ? Would this be a
possibility? Thanks
Linda Fox lfox1-at-wpo.it.luc.edu





From: cvierret-at-misn.com
Date: Thu, 24 Oct 1996 13:16:25 -0400 (EDT)
Subject: Lab listing

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In the spring a European journal asked for labs that wanted to publish their
abilities space in a North American listing of microscopic labs. We sent in a
listing and recieved a phone call from England about viewing the listing. The
number that we got off the answering machine keeps giving us an out of order
number. If any one has the number then please share it with us. Thank you,
Clarissa Vierrether
ORES
1200 West Third
Salem, MO 65560
cvierret-at-misn.com




From: pqmiragl-at-cc10ss.unity.ncsu.edu
Date: Thu, 24 Oct 1996 12:30:38 +0000
Subject: STEM

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To Whom It May Concern,

I am a graduate student at NC State University in an SEM class. I am
currently performing a research project on the development and
history of the scanning transmission electron microscope (STEM).
I was wondering if someone could give me an idea of what break-
throughs have been made over the past few decades and possibly
what references I could look for that might concern these
breakthroughs.

Thank You,

Peter Miraglia




From: Joseph P. Neilly 847-938-5024 :      NEILLY.JOSEPH-at-igate.pprd.abbott.com
Date: Thu, 24 Oct 1996 08:19:00 -0500 (CDT)
Subject: RE: Particle Atlas on CD-ROM

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Mr-Received: by mta RANDD; Relayed; Thu, 24 Oct 1996 08:21:51 -0500
Mr-Received: by mta MCM$RAND; Relayed; Thu, 24 Oct 1996 08:21:52 -0500
Mr-Received: by mta RANDD; Relayed; Thu, 24 Oct 1996 08:22:03 -0500
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Brad,

We have a copy of the Particle Atlas on CD-ROM and use it fairly
regularly. We also do a lot of light microscopy and electron microscopy
similar to what was done in the atlas. I do wish there was away to add
images and data to the atlas that are more typical of the types of
particles we encounter. This feature would make it an extremely
valuable resource but would likely change the atlas into a subscription.
Either way we felt it was worth the price we paid which I think was
$950 (we got a show special from the Intermicro meeting in '95). It
would also be nice if it were available in a Mac version.

Joe Neilly
Abbott Laboratories
Abbott Park, IL 60064





From: cvierret-at-misn.com
Date: Thu, 24 Oct 1996 13:16:22 -0400 (EDT)
Subject: Alpha Pb

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Has anyone done any microscopy work on alpha Pb? This is lead that has low
alpha radiation and is useful in the electronics industry. We have some samples
of "regular" lead and low alpha lead. WE would like to know if there is a way
to distingush between the two microscopically. The alpha emissions test are
very costly and seem to have a delayed turn around time. Please contact me
directly at cvierret-at-misn.com. I will post the answers if there are any. Thank
you for your time, Clarissa




From: philippe.buffat-at-cime.uhd.epfl.ch (Philippe-Andre Buffat)
Date: Thu, 24 Oct 1996 16:16:49 +0100
Subject: STM/AFM pictures for lectures

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X-Sender: pabuffat-at-cimedec1.epfl.ch
Message-Id: {v02130506ae95376608f5-at-[128.178.98.14]}

Dear Dieter,

I am looking for some STM and AFM pictures to show to our students within
the frame of an electron miroscopy course. I intend to tell them that there
are also other microscopies than the classical light microscopy and the
TEM/SEM. I would also point out how pictures can be misleading if you don't
know anything about the technique used to get them and about their scale.

In one example, I am trying to get as much as possible images of
dislocations of any kind (vein on saguarro cactus, painting on wall,
mosquito's eye, TEM=8A). If by any chance do you have a scanning probe image
of a (edge) dislocation observed at the atomic/molecular scale on metals,
ceramics, semi-conductors, or on a biological cristal, would you accept to
send me a copy (a file by e-mail would be fine and probably the easiest, a
print on paper would also be nice).

Of course, any other pictures are also welcome.

Thanks in advance for your help and time. Best regards

Philippe Buffat

__________________________________________________________________
Philippe Buffat
Ecole Polytechnique Federale de Lausanne (EPFL)
Centre Interdepartemental de Microscopie Electronique
Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
E-mail: philippe.buffat-at-cime.uhd.epfl.ch, WWW URL http://cimewww.epfl.ch/
______________________________ Eudora F2.1 ___________________________






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Thu, 24 Oct 1996 10:26:23 -0500 (EST)
Subject: Re: Epon Blocks

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Message-Id: {199610241827.NAA09315-at-Sparc5.Microscopy.Com}
To: Microscopy messageslistserver {Microscopy-at-Sparc5.Microscopy.Com}

We don't usually have this problem, since we choose our molds with proper
release in mind. Polyethylene, as in BEEM products, give the easiest
release. Silicon rubber is also good and can be made better by spraying
with a release agent. Polypropylene can also be used and the epon will
release with mechanical pressure as with a hammer or a pair of pliers or
with a hacksaw. If you are using polstyrene, then you have troubles. Here
is where dipping in liquid nitrogen might help, although you have to hope
that your epon does not shatter right through the important part of the
specimen. Selection of mold material is the secret.

A friend who does SEM mostly but is doing more TEM asked me the
} other day why her epon blocks were sticking in the mold. She said that
} they were cutting them out with razor blades but it was getting costly. I
} also use razor blades and have cut myself numerous times when the blade
} stuck. I even ended up with stitches on two fingers from one of these
} incidents. I have heard of using liquid nitrogen to remove blocks but as
} I've never seen it done, I have no idea how this works. But I thought
} there must be a better way to remove blocks. Does anyone have any easier
} and safer methods for removing epon blocks from their molds?
}
} Thanks in advance!!
}
} Lesley Bechtold
}
}
}
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*****Home of the #1 Gators





From: Sara Miller :      saram-at-acpub.duke.edu
Date: Thu, 24 Oct 1996 16:43:32 -0400 (EDT)
Subject: Re: Mark Wall

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On 24 Oct 1996, Mark Wall wrote:

} Date: 24 Oct 1996 08:07:49 -0800
} From: Mark Wall {Mark.Wall-at-quickmail.llnl.gov}
} To: List server {microscopy-at-Sparc5.Microscopy.Com}
} Subject: Mark Wall
}
} Does anyone know of any commercial services providing sectioning and/or
} cryosectioning of bio materials near me in northern Calif.? Please respond by
} E-mail.
}
} Thanks,
}
} Mark A. Wall
} Lawrence Livermore National Lab
} Livermore, CA 94550
} 510-423-7162
}
Hi,
We do lots of sectioning and ultracryosectioning for lots of
people. Although we're not in No. CA, there is FedEX. We receive
samples by FedEx from all over for electron microscopy diagnostic virology
(can provide details on this too if anyone is interested). There's no
reason why you couldn't send cells/tissues in glutaraldehyde or
paraformaldehyde for processing. Let me know if we can help.

Sara

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: Sara Miller :      saram-at-acpub.duke.edu
Date: Thu, 24 Oct 1996 16:43:32 -0400 (EDT)
Subject: Re: Mark Wall

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


On 24 Oct 1996, Mark Wall wrote:

} Date: 24 Oct 1996 08:07:49 -0800
} From: Mark Wall {Mark.Wall-at-quickmail.llnl.gov}
} To: List server {microscopy-at-Sparc5.Microscopy.Com}
} Subject: Mark Wall
}
} Does anyone know of any commercial services providing sectioning and/or
} cryosectioning of bio materials near me in northern Calif.? Please respond by
} E-mail.
}
} Thanks,
}
} Mark A. Wall
} Lawrence Livermore National Lab
} Livermore, CA 94550
} 510-423-7162
}
Hi,
We do lots of sectioning and ultracryosectioning for lots of
people. Although we're not in No. CA, there is FedEX. We receive
samples by FedEx from all over for electron microscopy diagnostic virology
(can provide details on this too if anyone is interested). There's no
reason why you couldn't send cells/tissues in glutaraldehyde or
paraformaldehyde for processing. Let me know if we can help.

Sara

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: John Hunt :      hunt-at-msc.cornell.edu
Date: Thu, 24 Oct 1996 10:09:48 -0400 (EDT)
Subject: Re: Quantitative microscopy of packed particles (fwd)

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Contact Ken Hover for more info.


Forwarded message:
} From KCH7-at-cornell.edu Wed Oct 23 20:21:30 1996
} Message-Id: {2.2.32.19961024002431.00694f4c-at-postoffice4.mail.cornell.edu}
} X-Sender: kch7-at-postoffice4.mail.cornell.edu
} X-Mailer: Windows Eudora Pro Version 2.2 (32)
} Mime-Version: 1.0
} Content-Type: text/plain; charset="us-ascii"
} Date: Wed, 23 Oct 1996 20:24:31 -0400
} To: John Hunt {hunt-at-msc.cornell.edu}
} X-UIDL: 846158903.012
} From: "Kenneth C. Hover" {KCH7-at-cornell.edu}
} Subject: Re: Quantitative microscopy of packed particles (fwd)
}
} John:
}
} One of my colleagues at NIST has worked in this area. His name is Ken
} Snyder, working in the Building Materials Lab under Dr. Geoff Frohnsdorff.
} I am not in my office but can get address and email.
}
} ken
}
}
}
}
}
} At 09:41 AM 10/16/96 -0400, you wrote:
} } Forwarded message:
} } } From Microscopy-request-at-Sparc5.Microscopy.Com Tue Oct 15 15:42:36 1996
} } Message-Id: {s263847e.073-at-intergate.dot.gov}
} } X-Mailer: Novell GroupWise 4.1
} } Date: Tue, 15 Oct 1996 12:32:16 -0400
} } From: "N. Shashidhar" {NSHASHIDHAR-at-intergate.dot.gov}
} } To: Microscopy-at-Sparc5.Microscopy.Com
} } Subject: Quantitative microscopy of packed particles
} }
} } I need to quantify pavements, which can be looked at as a system of packed
} particles. I need to
} } obtain the number of contacts each particle has on average with another
} particle. This can then be
} } related to mechanical properties such as shear resistance, etc.
} }
} } Are there microscopic techniques to get this information from a compact and
} stereology methods to
} } extract such information. Any response will be appreciated.
} }
} } Thank You.
} }
} }
} Kenneth C. Hover, P.E., Ph.D.
} Associate Dean for Undergraduate Programs
}
} 221 Carpenter Hall
} Cornell University
} Ithaca, New York 14853
}
} Phone 607-255-0393/8340
} Fax 607-255-9606
} Email kch7-at-cornell.edu
}





From: P.V.Hatton :      P.V.Hatton-at-sheffield.ac.uk
Date: Thu, 24 Oct 1996 19:47:36 +0100
Subject: Immuno. LM/TEM and Ti rod - HELP

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Dear Colleagues,

I have a Postdoc. going over to Holland for a six month placement.
She wants to know where to find a reliable method to embed tissue
that contains ceramic or metallic implants - she hopes to preserve a
number of bone proteins in the process (Vitronectin, Fibronectin &
Osteopontin) for immuno. work in 1997. The questions are:

1. Do we have to use a cryo/freeze-sub. route to preserve this
tissue, or might we get away with a "light" glut. fixation?

2. Would LR Gold be an appropriate resin, bearing in mind me may
want to do LM and TEM?

Finally, does anybody know a source for commercially pure titanium
rod (diameter 1.5 to 2.0 mm)?

Thank you for your help. Paul


Dr Paul V. Hatton
Lecturer in Biomaterials
School of Clinical Dentistry
University of Sheffield
Claremont Crescent
SHEFFIELD S10 2TA

Tel. (0114) 271 7938
Fax. (0114) 2665326
or 2797050




From: J.K.Chen :      jkchen-at-mcmail.cis.mcmaster.ca
Date: Thu, 24 Oct 1996 19:58:28 -0400 (EDT)
Subject: Re: STM/AFM pictures for lectures

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Alternate-Recipient: allowed
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Microscopy-at-Sparc5.Microscopy.Com

Hi,

Not too long ago, I put up a homepage for a university open house
(, thus not designed for academic purpose,) which may have part of
what you need. The pages show how various scales can make a difference
to the microstructures observed. You can find these at:

http://mse.mcmster.ca/research/micro

Suggestions are welcome as well.

============================================================================
J. K. Chen, PhD | Postdoctoral Fellow
357 Engineering Bldg. | E-mail: jkchen-at-mcmaster.ca
McMaster University | Phone: +1-905-5259140 ext.27042 (O)
Dept. of Materials Sci. & Eng.| Fax: +1-905-5289295
Hamilton, ON L8S 4L7, CANADA | http://mse.mcmaster.ca/faculty/cv/jkchen
============================================================================

At 04:16 PM 10/24/96 +0100, you wrote to Microscopy listserver:
} ... snip ...
} I would also point out how pictures can be misleading if you don't
} know anything about the technique used to get them and about their scale.
} ... snip ...
}
} Of course, any other pictures are also welcome.
}
} Thanks in advance for your help and time. Best regards
}
} Philippe Buffat





From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Thu, 24 Oct 1996 14:12:43 -0500
Subject: Re: CPD free floating section

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Message-Id: {199610241904.OAA25994-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Greetings Friends, Can anyone help with a proceedure for CPD a
} 50um free floating section? It is brain tissue that has been fixed,
} cryosectioned and returned to buffer. All wizardry is welcome at
} this point !! Has anyone used Peldri II ? Would this be a
} possibility? Thanks
} Linda Fox lfox1-at-wpo.it.luc.edu

Linda;
CPD: cut the end off of a BEEM capsule, cut out the center of the
capsule's cap, cut off the cap of another BEEM capsule, cut out the center,
put plankton netting over the end of the capsule with the lid, put in
sections, close lid; dehydrate, standing open end up in a Wheaton 4 mL
vial, add EtOH to capsule, withdraw from vial outside of capsule; put on
cut-off lid with netting,
put in CPD & dry:

___/ \__
| /________________\ |
lid on 1st capsule ---} | | | | {-----lid, removed from 2nd
| | | |
| |________|_______| |
|__\ | /_|
\ | / {------plankton netting
|
BEEM

Peldri: fluorocarbon, not made anymore.
or: dehydrate as usual, and dry from HMDS (Hexamethyldisilizane).
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel *all mail:*
Microscopy
University of Illinois Station A
Rm 74 Bevier Hall PO Box 5037
905 S. Goodwin Ave. Champaign, IL 61825-5037 USA
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu
(217) 355-1143 home

*********** looking for a job again ***********






From: em-at-mediacity.com (Ed Monberg)
Date: Thu, 24 Oct 1996 18:20:16 -0700
Subject: Re: Alpha Pb

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At 1:16 PM 10/24/96, cvierret-at-misn.com wrote:
} Has anyone done any microscopy work on alpha Pb? This is lead that has low
} alpha radiation and is useful in the electronics industry. We have some
} samples
} of "regular" lead and low alpha lead. WE would like to know if there is a way
} to distingush between the two microscopically. The alpha emissions test are
} very costly and seem to have a delayed turn around time.


WHY COSTLY ? Alpha is easy to detect and assay, yes ?

Is there some OTHER difference ?







Regards,



(signed) Ed Monberg {em-at-mediacity.com}

--------------------------------------------------

510-429-1060 Fax 429-1065
LMDC, (Laser & Motion Development Co.)
3101 Whipple Road
Union City, CA 94587-1216

TO BE ON OUR LIST AND BE THE FIRST
TO KNOW ABOUT BARGAIN PRICED TECHNOLOGY EQUIPMENT,
Just send an e-mail containing
"Join Equipment List" in the header line.

Our web page: http://www.lasermotion.com
Our e-mail: office-at-lasermotion.com






From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Fri, 25 Oct 1996 10:56:35 +1000 (EST)
Subject: Re: Osmium waste recycling

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}
} What does everyone else do with their osmium tetroxide waste?

} For a few years I coordinated a scheme in Australia where anyone could send
me their Osmium waste. We precipitated it by adding formaldehyde, I stored
it until we had about 100 litres then we sent it to Johnson Matthey. They
filtered off the osmium precipitate and I think then sent the cake to
Matthey in the UK for refining. The recovery rate was much less than we
hoped (probably due to the volatile nature of the oxides) and out of the
value of the osmium that was recovered, most of the money went to pay the
refining cost. All we were left with was a warm fuzzy feeling that we had
helped recycled a scarce resource.

But do try it if you like. Contact MAtthey in the UK (They may be Matthey
Garrett, I cant track all these company name changes) as they certainly know
about refining osmium.

Mel Dickson





From: Jim Darley :      p&s-at-ultra.net.au
Date: Fri, 25 Oct 1996 11:27:58 +1000
Subject: Re: STM/AFM pictures for lectures

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {1.5.4.32.19961025012758.006a6384-at-mailhost.ultra.net.au}
X-Sender: pns-at-mailhost.ultra.net.au
X-Mailer: Windows Eudora Light Version 1.5.4 (32)
Mime-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable

At 16:16 24/10/96 +0100, you wrote:
} Dear Dieter,
}
} I am looking for some STM and AFM pictures to show to our students within
} the frame of an electron miroscopy course. I intend to tell them that there
} are also other microscopies than the classical light microscopy and the
} TEM/SEM. I would also point out how pictures can be misleading if you don't
} know anything about the technique used to get them and about their scale.
}
} In one example, I am trying to get as much as possible images of
} dislocations of any kind (vein on saguarro cactus, painting on wall,
} mosquito's eye, TEM=8A). If by any chance do you have a scanning probe=
image
} of a (edge) dislocation observed at the atomic/molecular scale on metals,
} ceramics, semi-conductors, or on a biological cristal, would you accept to
} send me a copy (a file by e-mail would be fine and probably the easiest, a
} print on paper would also be nice).
}
} Of course, any other pictures are also welcome.
}
} Thanks in advance for your help and time. Best regards
}
} Philippe Buffat
}
} __________________________________________________________________
} Philippe Buffat
} Ecole Polytechnique Federale de Lausanne (EPFL)
} Centre Interdepartemental de Microscopie Electronique
} Address: EPFL-CIME, Batiment MX-C, CH-1015 Lausanne, Switzerland
} Phone: +41(21)693 29 83 Fax: +41(21)693 44 01 (Central European Time)
} E-mail: philippe.buffat-at-cime.uhd.epfl.ch, WWW URL http://cimewww.epfl.ch/
} ______________________________ Eudora F2.1 ___________________________
Hello Philippe and whoever:
Within our on-line catalogue is an excellent collection of
microscopy related links. You will find numerous images to chose from in the
various Atomic/Tunnelling microscopy sites and endless more microscopy
images under the header "Image Resources". I expect that you know that you
can copy images to disk by clicking on the right mouse button. Images on the
internet are covered by copyright but you could ask to use these and many
posted images in fact specifically permit their use for teaching purposes.=
=20
Cheers
Jim Darley
=20
Probing & Structure
} (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia
}
} Phone +61 77 740 370 Fax: +61 77 892 313
} A great microscopy site http://www.ultra.net.au/~pns/





From: H.BRINKIES -SE108/TEL.8657 :      hbrinkies-at-swin.edu.au
Date: Fri, 25 Oct 1996 14:02:56 EST+10
Subject: LEITZ objectives

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Message-Id: {199610250402.AA09220-at-lucy.swin.edu.au}
Comments: Authenticated sender is {hbrinkies-at-gpo.swin.edu.au}

I know this server is mainly concerned with electron microscopy but I
need some information on optical equipment.

As most of us know university funds are getting constantly smaller. I
am therefore trying to refurbish am older Leitz micro-hardness tester
that had been stored in a cupboard for several years. The objectives
are badly damaged. Where can I find replacements ?
The objectives are marked A 0.18 C HM 25oo 10x and A 0.70 C HM 6.3
40x Ernst Leitz-Wetzlar respectively.

Any help is appreciated.

Hans Brinkies
SWINBURNE, University of Technology
School of Mechanical and Manufacturing Engineering
Industrial Microscopy
HAWTHORN, 3122, Australia




From: Keith Ryan :      KPR-at-WPO.NERC.AC.UK
Date: Fri, 25 Oct 1996 08:20:35 +0000
Subject: Osmium waste -Reply

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Message-Id: {s2707802.082-at-WPO.NERC.AC.UK}
X-Mailer: Novell GroupWise 4.1

Dear Malcolm

There was a short 'Technical Tip' in the procedings of the
RMS some time ago re. osmium disposal which I took to
heart! I bring in old cooking oil and tip it into that so that it
becomes bound and inactivated. When there is enough, I
mix it with a hot water / detergent mixture (Quadralene
Instrument Cleaner) and then wash down the sink with
'copious amounts of running water'. No matter what you do
with it, it has to end up in the environment from whence it
came?

With best wishes - Keith Ryan

++++++++++++++++++++++++++++++++++++++++++++++++++
Keith Ryan
Plymouth Marine Laboratory, Citadel Hill,
Plymouth, Devon PL1 2PB, England

Tel: ++44 1752 633294 (international)
01752 633294 (national)
Fax: ++44 1752 633102 (international)
01752 633102 (national)
e-mail: k.ryan-at-pml.ac.uk
PML web site: http://www.npm.ac.uk/pml
++++++++++++++++++++++++++++++++++++++++++++++++++





From: Dan Hill (Bioc) :      dh2-at-mole.bio.cam.ac.uk
Date: Fri, 25 Oct 1996 10:00:22 +0100 (BST)
Subject: Re: Epon Blocks

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To avoid cut fingers when slicing open embedding capsules I use a TAAB
capsule splitter. This is a metal block with a hole drilled at either end
to take a 6 or 8mm capsule and two slits on either side to enable a
single-edge razor blade to be inserted and drawn down the side of the
capsule without the risk of it slipping and slicing into your fingers.
The nearest distributor to you would be in Canada at Marivac Ltd, 5821
Russel Street, Halifax, Nova Scotia, B3K 1X5. It costs 26.72 UK pounds.

Dan Hill
Biochemistry Dept
Cambridge University
United Kingdom


On Thu, 24 Oct 1996, Lesley S. Smith wrote:

} A friend who does SEM mostly but is doing more TEM asked me the
} other day why her epon blocks were sticking in the mold. She said that
} they were cutting them out with razor blades but it was getting costly. I
} also use razor blades and have cut myself numerous times when the blade
} stuck. I even ended up with stitches on two fingers from one of these
} incidents. I have heard of using liquid nitrogen to remove blocks but as
} I've never seen it done, I have no idea how this works. But I thought
} there must be a better way to remove blocks. Does anyone have any easier
} and safer methods for removing epon blocks from their molds?
}
} Thanks in advance!!
}
} Lesley Bechtold
}
}




From: svepet :      svepet-at-ikp.liu.se
Date: Fri, 25 Oct 1996 14:04:37 +0200 (MET DST)
Subject: LaB6 on SEM

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I am a user of a JEOL6400 SEM with LaB6 gun. I have noticed that there is a
new cathode on the market called DENKA LKS7. Has anyone got any experience
in using this type of cathode.

Sten Johansson





From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Fri, 25 Oct 1996 09:10:01 -0400
Subject: Re: CPD free floating section

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Have you tried Hexamethyldisilizane (HMDS for short). We have found
it more convient than Peldri and it gives similar to better results. There
are discussions about both which came across the list and I have archived
them at the web site listed at the end of this message. Click on the "Tips &
Tricks " button and look in the SEM section. Let me know if you cannot
access it and I will get it to you some other way



At 10:10 AM 10/24/96 -0500, you wrote:
} Greetings Friends, Can anyone help with a proceedure for CPD a
} 50um free floating section? It is brain tissue that has been fixed,
} cryosectioned and returned to buffer. All wizardry is welcome at
} this point !! Has anyone used Peldri II ? Would this be a
} possibility? Thanks
} Linda Fox lfox1-at-wpo.it.luc.edu
}
}
}




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
GO GATORS
Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 352-392-1295
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "





From: kna101-at-utdallas.edu
Date: Fri, 25 Oct 1996 08:48:00 -0500 (CDT)
Subject: Re: Osmium waste -Reply

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Keith,

Your comment on no matter what you do with it, it has to end up
from whence it came sounds like what the big manufacturing companies
used to say when they were found dumping large volumes of untreated waste
into rivers and streams. These things are no longer in their natural
form and so they can do more harm. I like the idea of recycling
even if you only break even. At least you know it won't be showing
up in your drinking water or food in the near future...That's my two cents.

Karen



On Fri, 25 Oct 1996, Keith Ryan wrote:

} Dear Malcolm
}
} There was a short 'Technical Tip' in the procedings of the
} RMS some time ago re. osmium disposal which I took to
} heart! I bring in old cooking oil and tip it into that so that it
} becomes bound and inactivated. When there is enough, I
} mix it with a hot water / detergent mixture (Quadralene
} Instrument Cleaner) and then wash down the sink with
} 'copious amounts of running water'. No matter what you do
} with it, it has to end up in the environment from whence it
} came?
}
} With best wishes - Keith Ryan
}
} ++++++++++++++++++++++++++++++++++++++++++++++++++
} Keith Ryan
} Plymouth Marine Laboratory, Citadel Hill,
} Plymouth, Devon PL1 2PB, England
}
} Tel: ++44 1752 633294 (international)
} 01752 633294 (national)
} Fax: ++44 1752 633102 (international)
} 01752 633102 (national)
} e-mail: k.ryan-at-pml.ac.uk
} PML web site: http://www.npm.ac.uk/pml
} ++++++++++++++++++++++++++++++++++++++++++++++++++
}
}




From: H. ADAMS :      hadams-at-nmsu.edu
Date: Fri, 25 Oct 1996 09:04:58 -0600 (MDT)
Subject: EML support

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I would like very much to thank all those who responded to my
inquery/plea for help/ideas on placement and in house support for university
wide em facilities. The ideas were gobbbled up by our newly appointed
Users Committe. We are putting together our recommendations at the end of
next week to the central adminstration and then cross our fingers and wait.
Thanks Again,
Hank Adams
EML
New Mexico State University
Las Cruces, NM "The Green/Red Chile Capitol"




From: slc6-at-lehigh.edu (Sharon Coe)
Date: Sat, 26 Oct 1996 11:57:34 -0400
Subject: Ad for microscopy server

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Could you please place the following ad on the Microscopy Server?
Please let me know that you got this message.

Thank you.
*****************************************************************
SENIOR FACULTY POSITION IN MICROSCOPY/MICROANALYSIS/
MATERIALS SCIENCE
AND ENGINEERING

The Department of Materials Science and Engineering at Lehigh University
has an opening for a senior (associate/full) professor with expertise in
electron microscopy and/or microanalysis (SEM, TEM, AEM, etc.). The
successful candidate must have a proven record of research in the
application of microscopy and/or microanalysis to the solution of materials
problems and be able to conduct independent and cooperative research in
MS&E. Ability to teach undergraduate and graduate courses in microscopy
and materials is essential. Experience in running a microscopy facility
will be an advantage.
Curriculum vitae and the names of three references should be sent
by December 15, 1996 to:

Professor David B. Williams
Chairman, Search Committee
Dept. of Materials Sci. & Eng.
Lehigh University
5 East Packer Avenue
Bethlehem, PA 18015-3195

Lehigh University is an committed to recruiting, retaining, and tenuring
women and minorities.
***************************************************************************



Sharon L. Coe
Department of Materials Science & Engineering
5 East Packer Avenue
Lehigh University
Bethlehem, PA 18015
610/758-5133
e-mail: slc6-at-lehigh.edu







From: petford-at-vax.ox.ac.uk
Date: Fri, 25 Oct 1996 18:08:19 +0100
Subject: request for cryo-HREM images

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Dear Group, I have been asked to give a couple of lectures in February as part
of an interdisciplinary lecture course on 'The new microscopies'. I will be
discussing HREM, and since the lectures are aimed at both physical and
biological scientists I would very much like to include some life science
examples. I know that very good work has been done using cryo-HREM. If anybody
has any images that I would be able to use (suitably referenced of course) or
could suggest people to contact, I would be most grateful.


Yours,
Amanda Petford-Long


Dept. of Materials, Univ. of Oxford
Parks Road, Oxford OX1 3PH, UK
Tel +44 1865 273656
Fax +44 1865 283333




From: Glen MacDonald :      glenmac-at-deskmail.washington.edu
Date: Fri, 25 Oct 1996 09:56:21 -0200
Subject: Re: Epon Blocks

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"Lesley S. Smith" {lesleys-at-pobox.upenn.edu}
cc: microscopy-at-Sparc5.Microscopy.Com
In-Reply-To: {Pine.SGI.3.91.961025094722.29405A-100000-at-mole.bio.cam.ac.uk}
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The simplest way to avoid cuts while opening BEEM capsules is to set the capsule
upright on the bench, resting on its flat, uncapped end. Then, perhaps with the
middle finger of each hand supporting the capsule on the side away from you,
slide a razor blade down the near side. But, hold the razor blade tangentially
to the capsule. Removing a broad strip of plastic make the thing easier to open
and dump the block out, and allows better control over the blade motion.
}
} On Thu, 24 Oct 1996, Lesley S. Smith wrote:
}
} } A friend who does SEM mostly but is doing more TEM asked me the
} } other day why her epon blocks were sticking in the mold. She said that
} } they were cutting them out with razor blades but it was getting costly. I
} } also use razor blades and have cut myself numerous times when the blade
} } stuck. I even ended up with stitches on two fingers from one of these
} } incidents. I have heard of using liquid nitrogen to remove blocks but as
} } I've never seen it done, I have no idea how this works. But I thought
} } there must be a better way to remove blocks. Does anyone have any easier
} } and safer methods for removing epon blocks from their molds?
} }
} } Thanks in advance!!
} }
} } Lesley Bechtold
} }
} }

Glen MacDonald
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, Wa 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu





From: klivi-at-jhu.edu (Kenneth JT Livi)
Date: Fri, 25 Oct 1996 09:58:33 -0400 (EDT)
Subject: Re: EDXA of Ar

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In response to Scott's suggestion that an Ar standard could be made, it
seems to me that if you know your detector well enough and can determine
good k-factors for K and Cl, then you would be better off estimating the
ArX k-factor than trusting that you've properly manufactured and estimated
the concentration of the *standard*. DTSA is a program that would be
helpful in this situation. It takes some effort to fully characterize the
efficiency of your detector and then choose which set of empirical
parameters work in your analytical environment (e.g., ionization cross
sections, etc.), but it has been worthwhile for our lab. Ion implanting of
Ar in C (or one of the sheet silicate minerals like pyrophyllite) could
give you the shape reference.
Good luck.

Ciao for now,
Ken


} I was following this topic because I once looked at some ion sputtered
} hydroxyapatite films that had Ar incorporated in them. I ignored it because I
} figured it would diffuse out eventually.
}
} Mark Blackford's answer gave me an idea for an approach to the solution to this
} problem. It is based on low energy ion implantation. The problem with Mark's
} answer is that you do not know the concentration of the Ar in the graphite from
} the ion milling process and therefore cannot calculate the k-factor. You would
} also be calculating the k(C,Ar) factor and would be tough to correlate it with
} other k(C,X) factors since carbon is tough to quantify in a lot of materials.
} Even though the graphite is tough to ion mill with straight Ar you will still
} lose C while you mill. You need to use a material that resists ion milling
} from Ar and was not prepared by ion milling. I suggest using W. The W could
} easily be electrolytically prepared with 5% NaOH solution (5V ac) and the TEM
} foils implanted with Ar at about 1 keV. (It would be better to prepare the W
} with a known geometry such as would result by Tripod Polishing.) The
} concentration profiles could be calculated using some of the Monte Carlo TRIMML
} codes or other codes that are available. Unfortunately, they are not great at
} low energies. Experimental alternative are to do depth profiling of implanted
} field emitters in an atom probe(Been there, done that.), SIMS profiles, or RBS
} profiles. If you know the thickness of the W sample and the concentration
} profile of the implanted Ar, you could calculate the concetration of the Ar in
} the volume probed in the AEM. You could then calculate the k-factors from the
} measured intensities. A benefit of using W is that you can work with
} relatively thick samples without worrying too much about the thin film
} criteria. You are going to have large errors with this approach.
}
} Other materials that might work would be thin films of Au or Au/Pd that were
} ion implanted.
}
} This is not an easy problem. These are some thoughts off the top of my head,
} so you should take them as such.
}
} - -Scott Walck
}

Kenneth JT Livi
Department of Earth and Planetary Sciences
34th and Charles Streets
The Johns Hopkins University
Baltimore, Maryland 21218

klivi-at-jhu.edu (e-mail)






From: gwe-at-biotech.ufl.edu (Greg Erdos)
Date: Fri, 25 Oct 1996 14:09:06 -0500 (EST)
Subject: Re: EDXA of Ar

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I would like to be in touch with someoen in the S.F. Bay area that is doing
freeze-fracture electron microscopy and could do ultrarapid freezing of
uncryoprotected sample. I have a collaborator there and it would be easier
if he could work with some one locally.

Reply directly and not to the server.

Thank you/.
*******************************************************
Greg Erdos Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*****Home of the #1 Gators





From: walambe-at-erenj.com (William A. Lamberti)
Date: Fri, 25 Oct 1996 11:15:39 -0400
Subject: Quantitative microscopy of packed particles

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Ken:

The best technique for this would likely be xray tomography. One
can obtain 3d images of samples with 1 to 10 um resolution.
Element-specific maps can also be made, so one could obtain both
morphological & elemental info in 3d. Performing such experiments is best
done at a dedicated facility (beamlines; such as Brookhaven or NIST).
Depending upon the analysis needs though, bench-scale measurements using
more standard xray generators can produce very useful images.

Once the images are in hand, one simply uses standard image
processing techniques to extract the desired parameters (contact points,
contact areas, angles, etc.).

Good luck.

Regards, Bill.




} Subject: Quantitative microscopy of packed particles
}
} I need to quantify pavements, which can be looked at as a system of
} packed particles. I need to obtain the number of contacts each } particle
has on average with another particle. This can then be related } to
mechanical properties such as shear resistance, etc.
}
} Are there microscopic techniques to get this information from a } compact
and stereology methods to extract such information. Any } response will be
appreciated.
}
} Thank You.
}
}
} Kenneth C. Hover, P.E., Ph.D.
} Associate Dean for Undergraduate Programs
}
} 221 Carpenter Hall
} Cornell University
} Ithaca, New York 14853
}
} Phone 607-255-0393/8340
} Fax 607-255-9606
} Email kch7-at-cornell.edu
William A. Lamberti
Office LA-196
Exxon Research and Engineering Company
Route 22 East
Annandale, NJ 08801
(908)730-2144 office
(908)730-2262/2104 labs
(908)730-3042/3051 fax
Email: walambe-at-erenj.com






From: BARRYSCAN-at-aol.com
Date: Fri, 25 Oct 1996 17:06:19 -0400
Subject: subscribe

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Could you please give me information on how to subscribe.

Thanks ,
L.Barry




From: shaf :      mshaf-at-darkwing.uoregon.edu
Date: Fri, 25 Oct 1996 14:30:15 -0700
Subject: Re: EDXA of Ar

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At 09:58 AM 10/25/96 -0400, you wrote:
} In response to Scott's suggestion that an Ar standard could be made, it
} seems to me that if you know your detector well enough and can determine
} good k-factors for K and Cl, then you would be better off estimating the
} ArX k-factor ...

This suggestion reminds me of an ancient method of synthesizing peaks
given only a few measured spectra. The idea was, for a given keV and
detector low-energy efficiency, acquire a spectrum of pure carbon (e.g.,
from polished vitreous carbon or diamond) and assume this spectrum defines
the envelope (shape) for all spectral peaks for the pure elements.
Kenneth's suggestion of measuring K and Cl, together with the C
"efficiency" spectrum would provide excellent criteria for knowing exactly
what the Ar intensity should be.

cheers, shaf

{\/} /\ {\/} /\ {\/} /\ {\/} cognito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/}
Michael Shaffer, R.A. - University of Oregon Electron Probe Facility
mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu
http://darkwing.uoregon.edu/~mshaf/epmahome/





From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Sat, 26 Oct 1996 12:29:08 +1000 (EST)
Subject: Epon sticking to molds

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I dont remember epon sticking to polythene or polystyrene if it was fully
cured. But epoxies will stick to silicone rubber molds when they get older.
Seems that silicone rubber molds are not fully cured so thye have reactive
groups free and you start to get the epoxies cross linking to the rubber.
Once that happens you have to throw out the silicone rubber mold and buy (or
cast) a new one.

mel dickson





From: Jim Darley :      p&s-at-ultra.net.au
Date: Sat, 26 Oct 1996 12:17:27 +1000
Subject: CPD free floating section

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} Date: Thu, 24 Oct 1996 10:10:41 -0500
} From: Linda Fox {lfox1-at-wpo.it.luc.edu}
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: CPD free floating section
}
} Greetings Friends, Can anyone help with a proceedure for CPD a
} 50um free floating section? It is brain tissue that has been fixed,
} cryosectioned and returned to buffer. All wizardry is welcome at
} this point !! Has anyone used Peldri II ? Would this be a
} possibility? Thanks
} Linda Fox lfox1-at-wpo.it.luc.edu
} **************************************
Linda & You:
Sorry Philip O. but Peldri II is available. (Ted Pella Inc & P&S)
Those compounds do nasty things to the Ozone layer but, I am afraid, my
motorcar alone could produce enough nitrous oxides to exceed the damage done
by all the Peldri in the world.
The technical issue is the fragillity and the preservation of the section
for this Peldri II would be may first choice.
Jim Darley

Probing & Structure
} (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia
}
} Phone +61 77 740 370 Fax: +61 77 892 313
} A great microscopy site http://www.ultra.net.au/~pns/





From: Kathy Barker :      barker-at-nucleus.immunol.washington.edu
Date: Fri, 25 Oct 1996 22:08:02 -0700
Subject: Please delete

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} Please deleted my name from the microscopy mailing list...til another time.
Good stuff.



Kathy Barker






From: Kathy Barker :      barker-at-nucleus.immunol.washington.edu
Date: Sat, 26 Oct 1996 07:29:14 -0700
Subject: Please delete

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} Please deleted my name from the microscopy mailing list...til another time.
Good stuff.



Kathy Barker








From: MUjiie-at-aol.com
Date: Sat, 26 Oct 1996 14:30:25 -0400
Subject: Unsubscribe

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Unscribe




From: lkerr-at-mbl.edu (Louis Kerr)
Date: Sun, 27 Oct 1996 17:53:45 -0500
Subject: Re: Epon molds - casting?

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Mel,

Do you know of an established method for casting molds for epoxies. I need
to embed odd sized samples and have not been able to locate the appropriate
sized molds so your idea of casting a mold sounds interesting!

Thanks,
Louie Kerr

At 12:29 PM 10/26/96, Dr Mel Dickson wrote:
} I dont remember epon sticking to polythene or polystyrene if it was fully
} cured. But epoxies will stick to silicone rubber molds when they get older.
} Seems that silicone rubber molds are not fully cured so thye have reactive
} groups free and you start to get the epoxies cross linking to the rubber.
} Once that happens you have to throw out the silicone rubber mold and buy (or
} cast) a new one.
}
} mel dickson

Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)

VISIT OUR WEB SITE:
http://www.mbl.edu






From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Mon, 28 Oct 1996 13:13:50 +1100 (EST)
Subject: Re: Epon molds - casting?

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} Mel,
}
} Do you know of an established method for casting molds for epoxies. I need
} to embed odd sized samples and have not been able to locate the appropriate
} sized molds so your idea of casting a mold sounds interesting!
}
} Thanks,
} Louie Kerr


Hi Louie,

The easy way to cast a mold is to use silicone encapsulating rubber. We
used RTV E mould making rubber we bought from a plastic supplies shop here.
You make replicas exactly the shape of the final cast you want, then seal
them (say with superglue) to the bottom of a shallow dish (a glass Petri
dish is ideal. The replicas should have a smooth surface. You can punch
numbers or letters on them if you want. Mix up the RTV resin as per
instructions, degas in a vacuum desiccator and pout it into the dish and
leave it to cure again as per instructions.

The silicone eventually becomes hard, brittle and adheres to the epoxies so
be ready to make more molds as this happens.

Incidently, if you want to see through the roughened surface of an
embedment, just smear a thin film of immersion oil over it.

Mel dickson





From: Mark Aindow :      aindowm-at-sun1.bham.ac.uk
Date: Mon, 28 Oct 1996 11:26:47 GMT
Subject: Postdoctoral Position Available

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Dear Members of the list,

The following is the text of a advertisement which will appear in
next weekUs issue of the New Scientist magazine. I would be grateful
if you could bring this to the attention of any of your students or
colleagues who might be interested in applying for this post.

Thank you for you help in this matter, Mark Aindow

*********************************************************


THE UNIVERSITY OF BIRMINGHAM
School of Metallurgy & Materials


Research Fellow

The Nucleation Stage of Carbon Deposition
on Oxidised Stainless-Steel Surfaces



Applications are invited for the above industrially funded post which
will be available for two years. The work will involve producing
carbon deposits on oxidised stainless-steel surfaces from carbon
monoxide/dioxide mixtures and characterising them using high
resolution techniques including TEM and AFM. Applicants should have
a PhD and experience of relevant experimental techniques. Starting
salary will be in the range 14,317 - 15,986 pounds sterling per annum.

Preliminary enquiries should be directed to:
Dr. M. Aindow Telephone: 0121 414 5188, Email: M.Aindow-at-bham.ac.uk.

Application forms (returnable by 25 November 1996.) and further
particulars are available from the Director of Staffing Services,
The University of Birmingham, Edgbaston, Birmingham B15 2TT,
Telephone 0121 414 6483 (24 hours), (Email: Staffing-at-bham.ac.uk).

Please quote reference G19167/96A

**********************************************************************




*********************************************************************

Mark Aindow,
School of Metallurgy and Materials, Telephone; (0121) 414 5188
The University of Birmingham, FAX; (0121) 414 5232
Elms Road, Edgbaston, Birmingham, Email; M.AINDOW-at-BHAM.AC.UK
GB B15 2TT, United Kingdom.

*********************************************************************





From: Brian Burgess :      burgbr-at-che.ufl.edu
Date: Mon, 28 Oct 1996 08:37:46 -0500
Subject: unsubscribe

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From: Marilyn Wadsworth :      mwadswor-at-zoo.uvm.edu
Date: Mon, 28 Oct 1996 11:13:42 -0500 (EST)
Subject: Safranin O

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Dear Users Group,
We have a researcher interested in using Safranin O to dye bone/
cartilage sections embedded in Epon and cut at 4 microns. Has anyone
done this successfully, and if so how? Any help would be appreciated.

Regards,

Marilyn Wadsworth
mwadswor-at-zoo.uvm.edu




From: Edward Preble :      eapreble-at-eos.ncsu.edu
Date: Mon, 28 Oct 1996 13:25:43 -0500
Subject: polymer fibers

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Message-ID: {3274FAA7.6787-at-eos.ncsu.edu}

I had a question concerning the imaging of coated and non-coated
polymer fibers in scanning electron microscopy. If you could give me
any useful websites and/or journal articles that are related to this
topic it would be greatly appreciated.
Susan Lloyd
salloyd-at-eos.ncsu.edu




From: Xianying Burany :      xianying-at-udel.edu
Date: Mon, 28 Oct 1996 14:24:28 -0500 (EST)
Subject: Buehler Ltd.

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Good afternoon.

I am looking for the phone number of Buehler Ltd. Please reply me if you
know.

Thank you in advance.

Sandy
94765-at-udel.edu





From: tania-at-dynamotive.com (Tania Jones)
Date: Mon, 28 Oct 1996 11:44:34 -0800
Subject: determining % oxide on surface

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Hello,

Often I am required to determine how clean a sample of strip steel is by
comparing the amount of oxide remaining on the surface to the area scanned
on SEM. The method we currently use is extremely time-consuming and uses up
many polaroid films.
Does anyone know of a simple way of finding the ratio of oxide left on a
surface to bare metal? Is there any software available that may be of help?

Thanks in advance,

Tania Jones

tania-at-dynamotive.com





From: kszaruba-at-MMM.COM (Karen S. Zaruba)
Date: Mon, 28 Oct 1996 13:43:38 -0600
Subject: Re: TEM immunohistochemistry

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Please reply to the group. I would also find these references interesting.
(I know from some work at the light level on fixed tissue that there is
often a ton of nonspecific staining in the stratum corneum. Fortunately I
was interested in the dermis. But how do people get around this?)

Karen


} To all
} Is there anyone who has experience of TEM immunohistochemistry on human skin?
} Can he send us the methods used and is observations on cryofixation,
} cryoprotection and so on?
}
} Thanks you.
}
}
} Prof. Paolo Castano
} Istitute of Anatomy
} Via Mangiagalli 31, 20133 Milan
} Italy
} E-mail clsmteam-at-imiucca.csi.unimi.it
}

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
3M Company
3M Center 270-1S-01 Phone: 612-737-2971
St. Paul, MN 55144-1000 Fax: 736-1519

These opinions are my own and may not represent those of 3M.







From: Scott Holt :      102467.2752-at-CompuServe.COM
Date: 28 Oct 96 18:10:19 EST
Subject: BUEHLER's Phone No.

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Message-Id: {199610282129.QAA17083-at-thomas2.ge.com}

Dear Sandy at udel.edu,

BUEHLER's phone numbers are as follows:
(800)323-9330 and (847)295-6500.
Fax: (847)295-7929.

Please let me know if I can help further.

Best regards,
Scott D. Holt
BUEHLER
41 Waukegan Rd.
Lake Bluff, IL 60044
(847)295-4546

Manufacturers of scientific instruments, supplies and laboratory furniture
for use by microstructural analysts for over 60 years!





From: peggy-at-research.nj.nec.com (Peggy Bisher) (by way of Nestor J.
Date: Mon, 28 Oct 1996 18:39:52 -0500
Subject: Re: Immuno. LM/TEM and Ti rod - HELP

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Zaluzec)


} Finally, does anybody know a source for commercially pure titanium
} rod (diameter 1.5 to 2.0 mm)?
}

Try contacting:


GOODFELLOW

Web Page: http://www.goodfellow.com
Telephone: (800) 821-2870
Fax: (800) 283-2020
E-mail: inq-at-goodfellow.com




****************************************************************************

Margaret(Peggy) Bisher

NEC Research Institute _/ _/ _/_/_/ _/_/_/ _/
4 Independence Way _// _/ _/ _/ _/
Princeton, NJ 08540. _/ / _/ _/_/_/ _/ _/
Tel.: (609) 951-2629 _/ /_/ _/ _/ _/
Fax: (609) 951-2496 _/ _/ _/_/_/ _/_/_/ _/
e-mail: peggy-at-research.nj.nec.com


****************************************************************************






From: SCM!ATitkov-at-scmaust.attmail.com (SCM!ATitkov)
Date: Tue, 29 Oct 1996 09:06:43 +0800
Subject: ImageSlave

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Dear Microscopists,

Has anyone tried to use ImageSlave under Windows NT?

Alexander Titkov
SCM Chemicals Ltd.
PO Box 245
Bunbury WA 6231
Australia
Ph (097) 808 505






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Mon, 28 Oct 1996 10:31:14 -0600
Subject: Re: Epon Blocks

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Lesley & Glen -

Lesley didn't say specifically that she was using BEEM capsules. However, if
that's the case, I recommend the BEEM Capsule Press (E.F. Fullam, Inc.
cat.no. 54090; Structure Probe, Inc. cat.no. 2400-AB; Look in other
suppliers' catalogs under "capsule, embedding", or "BEEM".) We produce a lot
of blocks and this gizmo saves time as well as wear & tear on blocks and
fingers.

Joiner

At 09:56 AM 10/25/96 -0200, you wrote:
} The simplest way to avoid cuts while opening BEEM capsules is to set the
capsule
} upright on the bench, resting on its flat, uncapped end. Then, perhaps with the
} middle finger of each hand supporting the capsule on the side away from you,
} slide a razor blade down the near side. But, hold the razor blade tangentially
} to the capsule. Removing a broad strip of plastic make the thing easier to
open
} and dump the block out, and allows better control over the blade motion.
} }
} } On Thu, 24 Oct 1996, Lesley S. Smith wrote:
} }
} } } A friend who does SEM mostly but is doing more TEM asked me the
} } } other day why her epon blocks were sticking in the mold. She said that
} } } they were cutting them out with razor blades but it was getting costly. I
} } } also use razor blades and have cut myself numerous times when the blade
} } } stuck. I even ended up with stitches on two fingers from one of these
} } } incidents. I have heard of using liquid nitrogen to remove blocks but as
} } } I've never seen it done, I have no idea how this works. But I thought
} } } there must be a better way to remove blocks. Does anyone have any easier
} } } and safer methods for removing epon blocks from their molds?
} } }
} } } Thanks in advance!!
} } }
} } } Lesley Bechtold
} } }
} } }
}
} Glen MacDonald
} Virginia Merrill Bloedel Hearing Research Center
} Box 357923
} University of Washington
} Seattle, Wa 98195-7923
} (206) 616-4156
} glenmac-at-u.washington.edu
}
}
}





From: Leah Dobbs :      leadob-at-execpc.com
Date: Mon, 28 Oct 1996 20:26:41 -0000
Subject: RE: polymer fibers

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Message-ID: {01BBC50F.652DC3E0-at-voga-12.execpc.com}
"Microscopy-at-Sparc5.Microscopy.Com" {Microscopy-at-Sparc5.Microscopy.Com}

I would also be interested in resources for this topic.
Leadob-at-execpc.com
Leah dobbs





From: I.Ivanov-at-ix.netcom.com
Date: Mon, 28 Oct 1996 15:56:37 -0800
Subject: Re: determining % oxide on surface

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On 10/28/96 11:44:34 you wrote:
}
} Hello,
}
} Often I am required to determine how clean a sample of strip steel is by
} comparing the amount of oxide remaining on the surface to the area scanned
} on SEM. The method we currently use is extremely time-consuming and uses up
} many polaroid films.
} Does anyone know of a simple way of finding the ratio of oxide left on a
} surface to bare metal? Is there any software available that may be of help?
}
} Thanks in advance,
}
} Tania Jones
}

Dear Tania:
There is an ASTM standard protocol for QA/QC analysis of the surface oxide
layer on electropolished stainless steel using SEM, Auger and XPS. It
reports density of defects, average thickness of oxide layer (in Angstroms),
and Cr/Fe ratio in oxide. Some procedures in this protocol could be applied
to your samples.
We provide SEM/EDS,Auger,SIMS,TEM,GCMS,FTIR,HPLC,ICP-MS analytical services.
Igor Ivanov
RJ LeeGroup
530 McCormick Str.
San Leandro, CA 94577
510-567-0480 ph. 510-567-0488 FAX





From: John Best :      jbest-at-vicon.net
Date: Sat, 26 Oct 1996 20:18:44 -0700
Subject: Re: BS detectors

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Message-ID: {3272D494.7B37-at-vicon.net}

Hello All.............

I'm sorry to join the discussion late, but I haven't checked my email for
about a week.

I didn't see any replies recommending the micro-chanell plate as a
solution to the low kEv situation. My last impression of these systems
was that they were state of the art. They work very much like a
photo-multiplier, and provide the high gain and very good signal to noise
ratio.

If one were seeking a BSE system for low accellerating voltage
situations, it would be worth contacting GW electronics in Norcross, GA.
I have no interest in GW, other than seeing that the best technologies be
evaluated.

One comment about the micro-chanell: the last time I looked, they didn't
offer a topographic contrast mode (neither does the robinson). Why
doesn't someone build a micro-chanell plate with 2 or 4 separate detector
elements and high voltage supplies so the signals can be fed into a
traditional amplifier which offers atomic number and topographic
contrast? Sort of a multi-detector version of the micro chanell. Is
there a practical reason this can't be done, or is it a commercial
reason?

Regards,
John.

--
ELMDAS Co. P.O. Box 355, Alexandria, PA 16611
Voice: (814) 669-4474
Our website: http://www.vicon.net/~jbest
Email: jbest-at-vicon.net





From: Dr. Wayne Lanier :      lanier-at-slip.net
Date: Mon, 28 Oct 1996 21:51:05 -0800
Subject: unsubscribe

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----------------------------------
Dr. Wayne Lanier
250 Ashbury
San Francisco, CA 94117
[415] 346-4940
lanier-at-slip.net
---------------------------------






From: Dr Eric Lachowski :      che136-at-abdn.ac.uk
Date: Tue, 29 Oct 1996 10:53:34 +0000 (GMT)
Subject: EDX of Ar

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Thanks to all who responded to my recent query on the
EDX analysis of Ar.

Dr Eric Lachowski
University of Aberdeen
Department of Chemistry
tel +44 1224 272934
fax +44 1224 272921





From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Tue, 29 Oct 1996 09:00:49 +0000
Subject: Re: determining % oxide on surface

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} Hello,
}
} Often I am required to determine how clean a sample of strip steel is by
} comparing the amount of oxide remaining on the surface to the area scanned
} on SEM. The method we currently use is extremely time-consuming and uses up
} many polaroid films.
} Does anyone know of a simple way of finding the ratio of oxide left on a
} surface to bare metal? Is there any software available that may be of help?
}
} Thanks in advance,
}
} Tania Jones
}
} tania-at-dynamotive.com

Don't quite understand the problem - I'd have thought that any cleaned
steel surface would immediately oxidise on exposure to air, if only to a
monolayer.

I guess you're talking about a fairly thick corrosion-type of oxide layer?
For a reasonably flat surface, how about either an EDX map on the O-K peak,
or z-contrast image via BSE (you'd probably need to adjust HT to optimise
contrast depending on thickness of oxide layer)

Regards,
Larry Stoter






From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Tue, 29 Oct 1996 09:00:54 +0000
Subject: Re: EDX semi-quant analysis on cubes

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} I have a question about standardless semi-quant work that I am doing,
} involving take off angle. I have a TN5500 EDX instrument. When I analyze

snips

} My problem is, how do I perform standardless semi-quant work on the
} cubes within my powder, to examine homogeneity within the sample from one
} cube to the next?
}
} Thank You in advance.
} Mark Darus
} General Electric

First, I'd try running the quant programme on the same spectrum a number of
times but with different values for the relevant angles. This will give you
a feel for how sensitive your quant algorithm and particular combination of
elements is to these paramenters.

You might find that for sample tilts over a certain value (sensitivity to
specimen tilt reduces as the tilt increase - at high tilt, a small change
in tilt will have less effect on X-ray path lengths, so the absorption
correction will be changing less), the error is acceptably small - in which
case you can choose cubes appropriately. That is, select/set up high (but
not too high) tilt surfaces to analyse. I don't suppose the tilt angles of
the cubes will be determined by composition. (At this point you can also
check how good the algorithm is - how does it handle specimen tilts around
90 deg?)

It'll also give you an idea of how accurately you need to know the actual
tilt angles. Again, for low tilts, you'll probably need to know to within a
degree or so but I'd bet that at higher tilts (45 to 60 deg) you may get
away with knowing to the nearest 10 deg. If you do need to know the
specimen surface tilt angles 'accurately', then I guess some sort of stereo
pair procedure will give it to you?

I guess I'd be more worried about excitation volumes and X-ray paths to the
detector that exit the sides of the cubes rather than the 'top' surface.
This could be a problem irrespective of the tilt of the cubes, although
you'll minimise it by rotating the specimen so that the surface you are
analysisng is facing towards the detector and minimising the HT (to reduce
excitation volume). It will also depend on the composition - if it's mainly
low z, then the problem would be worse. Does the size of these cubes vary a
lot and is it sufficient to check how apparent composition varies with
size? If so, you might be able to work out a statistical extrapolation to
bulk composition, although you'll need to analyse a lot of 'randomly'
oriented cubes to get any sort of accuracy.

More practically, how much does the composition appear to vary and how
accurately do you really need to know it? For example, if the apparent
variation is within the error limits of the analysis anyway, then your
problem dissappears! Alternatively, if you analyses a fairly large number
of cubes (which you should be doing anyway, just like everbody else, to get
good statistics .......:)...) can you demonstrate that there are clearly 2
or 3 seperate populations? If so, this might 'solve' your real pratical
problem without even getting into the complexities of trying to do an
accurate analysis.

Regards,
Larry Stoter






From: Naresh Shah :      naresh-at-service1.uky.edu
Date: Tue, 29 Oct 1996 08:55:18 -0500
Subject: Re: Buehler Ltd.

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Message-ID: {32760CC6.3F64-at-pop.uky.edu}

Xianying Burany wrote:
}
} Good afternoon.
}
} I am looking for the phone number of Buehler Ltd. Please reply me if you
} know.
}
} Thank you in advance.
}
} Sandy
} 94765-at-udel.edu


1-800-Buehler
1-800-283-4537
and if you are looking for a competitor-
1-888-Struers
1-888-787-8377

--

Naresh Shah
Research Associate Professor, University of Kentucky
Consortium for Fossil Fuel Liquefaction Science (CFFLS) and
Department of Chemical and Materials Engineering (CME)
533 South Limestone Street, Room 111
Lexington, KY 40506-0043
Phone: (606) 257-5119; FAX: (606) 257-7215; e-mail: naresh-at-pop.uky.edu




From: Joergen Bilde-Soerensen 5802 :      j.bilde-at-risoe.dk
Date: Tue, 29 Oct 1996 15:13:35 +0200
Subject: Re: Pulstar

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Beat Frey wrote:
{I am wondering whether any Listserv subscribers have any experience
{with the new PULSSTAR preferably in biological applications
{that might help us guide our decision.

Dear Beat,

We are a materials science laboratory - but anyway: We have had very
good experience with the PULSTAR digital pulse processor. The
increased count rate is very useful - in particular when you are
doing linescans or X-ray mappings.

If you are working at high count rates you will encounter some
deterioration of the energy resolution; but in the cases where this is a problem,
you still have the possibility of choosing a low count rate with the
normal energy resolution.

Best wishes,
Joergen



-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
J. B. Bilde-Soerensen
Materials Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 35 11 73




From: Marc C. Brande, MS, Founder :      mcbrande-at-sierra.net
Date: Tue, 29 Oct 1996 08:01:18 +0000
Subject: Scanner for Histological slides

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Message-Id: {3275B9CE.52E2-at-sierra.net}
nih-image-at-soils.umn.edu

Can someone please point me to make/model/contact info for scanners
that will digitize directly standard glass microscope slides that
contain histological sections? Thanks so much.

Marc




From: Rachel Teitelbaum :      teitelba-at-aecom.yu.edu
Date: Tue, 29 Oct 1996 10:44:41 -0500 (EST)
Subject: immunohistochemistry on epon blocks

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I'm trying to get an antibody to work on glut fixed, osmicated, epon
blocks. I have a very high background of the secondary binding to the
sections on the copper grids, and cannot discern if the antibody is
working at all, beyond the background "noise". Has anyone been
successful with this, or am I wasting my time by trying to get this to
work. For my purposes, I cannot use lowicryl, or non-osmicated tissue.
Any suggestions?

Thanks,
Rachel




From: tania-at-dynamotive.com (Tania Jones)
Date: Tue, 29 Oct 1996 08:44:09 -0800
Subject: determining % oxide on surface

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Thank you for your responses, but I fear I may have posed the wrong
question. I am looking for oxide on the surface in more of an image
analysis point-of-view, than quantifying the thickness of the oxide.
I am considering the oxide to be like paint, and want to find the
percentage of the wire's surface uncovered by the "paint". Typically,
we have taken several photographs of the surface and crudely calculate
the surface covered in oxide.

I hope I've explained myself a little better this time.

Thanks in advance,

Tania Jones

tania-at-dynamotive.com





From: Richard Miller :      LYMFNOD-at-worldnet.att.net
Date: Tue, 29 Oct 1996 08:28:25 -0800
Subject: Re: Scanner for Histological slides

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Message-ID: {327630A9.5B58-at-WORLDNET.ATT.NET}
microscopy-at-Sparc5.Microscopy.Com, nih-image-at-soils.umn.edu

Marc C. Brande, MS, Founder wrote:
}
} Can someone please point me to make/model/contact info for scanners
} that will digitize directly standard glass microscope slides that
} contain histological sections? Thanks so much.
}
} Marc

I understand Polaroid makes one called Path Enabler. I have no personal
experience with the product.
--
Richard Miller M.D.
Hematopathology
"alles ist nur ein ubergang"





From: Christian Broesamle :      broesam-at-hifo.unizh.ch
Date: Tue, 29 Oct 1996 19:03:08 +0100
Subject: quatitative immunohistology

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Message-Id: {327646DC.52C9-at-hifo.unizh.ch}

Hello all,

Does anybody know of accepted procedures to quatitatively evaluate
immunehistochemical stainings. I am especially interested in evaluation
methods for enzyme driven detection which has the problem of possible
nonlinear relationships between signal and antigen abundance. Are there
references to this problem?

Christian
--
************************************************************
Christian Broesamle email: broesam-at-hifo.unizh.ch
Brain Research Institute tel: +41 1 385 6333
University of Zurich fax: +41 1 422 2262
CH-8029 Zurich http://www.hifo.unizh.ch
Switzerland
************************************************************




From: Woody.N.White-at-mcdermott.com
Date: 10/28/96 1:44 PM
Subject: determining % oxide on surface

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Message-Id: {199610292050.OAA03808-at-Sparc5.Microscopy.Com}


Tania,

Wonder if you have (and what kind) an EDS system? One with a light
element detector (for O)? Some EDS sys will let you compare spectra/search
a list for best fit. If you can obtain (standards) references of metal
with various oxide film thicknesses, you could (using identical acquisition
parameters) then compare the unknown spectrum to a series of references for
the best fit. Once standards are collected, this should be quick and easy.

Another possibility is quantitative BSE. This would require similar
standards. Digital image collection and manipulation would help quite a bit.
Comparing the BSE coefficients (brightness at same op. parameters) to the
standards will let you pick the closest film thickness. This method,
depending on the oxide thickness, may require the use of 10 kV, 5kV, or less.

In either case the specimens will have to be "squeaky" clean to avoid false
indications.

Have Fun!... Woody White
Babcock & Wilcox Research
Also -at-: woody.white-at-worldnet.att.net
http://www.geocities.com/capecanaveral/3722
______________________________ Reply Separator _________________________________


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Hello,

Often I am required to determine how clean a sample of strip steel is by
comparing the amount of oxide remaining on the surface to the area scanned
on SEM. The method we currently use is extremely time-consuming and uses up
many polaroid films.
Does anyone know of a simple way of finding the ratio of oxide left on a
surface to bare metal? Is there any software available that may be of help?

Thanks in advance,

Tania Jones

tania-at-dynamotive.com




From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Tue, 29 Oct 1996 14:39:33 -0500
Subject: Order

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Hi chris. Sorry for the delay in getting you this. As we spoke about
before I will need the Photoshop 4.0 upgrade for the PC. I don't know if it
is relevant or not but I am running win95. The serial # you requested
is"PWW300B1126201-337"
Additionally I need:
Maxtor 2.0 G hard drive , catalog #DR4875, price $269.95.

Epson stylus color inkjet, #PR11427, price $379.95. If you
sell the ink, please send me a backup of each.
I would appreciate if you could e-mail me back the total as well.
This order was taken out of the MicroWarehouse catalog volume 40.0 so let me
know if there are any price differences.


Customer # 6646947
PO# L02034

Ship to:
Scott Whittaker
University of Florida
218 Carr Hall
Gainesville, FL 32653

Let me know if there is anything else you need and thanks.





} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
GO GATORS
Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 352-392-1295
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "





From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: 10/28/96 1:25 PM
Subject: polymer fibers

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Susan:

I believe that this forum is also very good source of information on this
topic if you could be a little more specific. Many of the subscribers to
this list have direct experience imaging polymer fibers, and could perhaps
help you with your questions.

Regards,

-Bob
********************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA 91711
ph: (909)399-1311
email: Bob_Citron-at-cc.chiron.com
********************************

______________________________ Reply Separator _________________________________


I had a question concerning the imaging of coated and non-coated
polymer fibers in scanning electron microscopy. If you could give me
any useful websites and/or journal articles that are related to this
topic it would be greatly appreciated.
Susan Lloyd
salloyd-at-eos.ncsu.edu




From: John Best :      jbest-at-vicon.net
Date: Sat, 26 Oct 1996 20:18:44 -0700
Subject: Re: BS detectors

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Message-Id: {n1365542316.66366-at-chdqm.sps.mot.com}

RE} } BS detectors 10/29/96

I have a microchannel plate on my 6300F JEOL. It performs extremely well in
both modes - compositional (A+B) and topographical (A-B), and in low KV
applications. It was purchased in 1995. If purchasing a new detector it is
well worth evaluating this type, I'm sure there is more than one manufacturer.
The one I have was built by Galileo Electro-Optics Corp. in Mass.
(800-648-1800).
No interest in the company.....just providing info.

**********************************************************
Jake Schaper
Product Analysis Lab
Advanced Digital Consumer Division
Motorola, Inc.
1300 N. Alma School Rd. Chandler, Arizona 85224
Mail Drop CH240
Phone 602-814-4756
**********************************************************


--------------------------------------

I'm sorry to join the discussion late, but I haven't checked my email for
about a week.

I didn't see any replies recommending the micro-chanell plate as a
solution to the low kEv situation. My last impression of these systems
was that they were state of the art. They work very much like a
photo-multiplier, and provide the high gain and very good signal to noise
ratio.

If one were seeking a BSE system for low accellerating voltage
situations, it would be worth contacting GW electronics in Norcross, GA.
I have no interest in GW, other than seeing that the best technologies be
evaluated.

One comment about the micro-chanell: the last time I looked, they didn't
offer a topographic contrast mode (neither does the robinson). Why
doesn't someone build a micro-chanell plate with 2 or 4 separate detector
elements and high voltage supplies so the signals can be fed into a
traditional amplifier which offers atomic number and topographic
contrast? Sort of a multi-detector version of the micro chanell. Is
there a practical reason this can't be done, or is it a commercial
reason?

Regards,
John.

--
ELMDAS Co. P.O. Box 355, Alexandria, PA 16611
Voice: (814) 669-4474
Our website: http://www.vicon.net/~jbest
Email: jbest-at-vicon.net


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From: Goran Drazic :      goran.drazic-at-ijs.si
Date: Tue, 29 Oct 1996 16:24:26 GMT+2
Subject: MCEM '97- Multinational Congress on Electron Microscopy

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Congress Announcement:
_ _ _ _ _ _ _ _ _ _ _ _ _

MULTINATIONAL CONGRESS ON ELECTRON MICROSCOPY

MCEM '97

5 to 8 October 1997

Portoroz, Slovenia

http://www2.ijs.si/~k5www/MCEM97/index.html


1993 Parma, Italy ... 1995 Stara Lesna, Slovakia ... 1997 Portoroz, Slovenia


Organized by:

Slovenian Society for Electron Microscopy
Austrian Society for Electron Microscopy
Croatian Society for Electron Microscopy
Czechoslovak Society for Electron Microscopy
Microscopy Society of Hungaria
Italian Society for Electron Microscopy
_ _ _ _ _ _ _ _ _ _ _ _ _


DATE AND LOCATION

MCEM 97 will be held from Sunday 5 to Wednesday 8 October, 1997
at Grand Hotel Emona Convention Centre, Portoroz, Slovenia (EU).


SCOPE OF THE CONGRESS

The aim of the Congress, which is held under the auspices of CESEM,
is to allow the scientist to exchange the most recent results and technical
advances in the development and application of electron microscopy.

The main topics are:

Ultrastructural localization of molecules
Cryotechniques
EM in cell structure and function
EM in pathology
EM of microorganisms
New methods in analytical microscopy
EM in material science
Surface analysis
Image analysis
Digital processing and stereology
Instrumentation and tools


SCIENTIFIC PROGRAM

The program will consist of invited lectures, oral presentations, poster
presentations, and round table discussions. A limited number of
abstracts will be selected for oral presentations.


ABSTRACTS

All those intending to participate at the Congress are welcome to
submit an abstract.
Deadline for abstract submission is April 30, 1997.
Details for the format of the abstracts will be given in the Second
circular (January 1997).


TIME SCHEDULE

January 1997: Second Circular and call for papers
April 1997: Deadline for abstracts
July 1997: Deadline for registration


CONGRESS LANGUAGE

The official language of the Congress will be English.


EXHIBITION

Ample space, immediately adjacent to the lecture and poster rooms,
will be available for the exhibition of equipment, leaflets and books.
Interested companies should contact the Congress Secretariat.


ADDRESS OF THE CONGRESS SECRETARIAT:

MCEM'97
Ceramics Department
"Jozef Stefan" Institute
Jamova 39, 1000 Ljubljana
Slovenia

Tel.: +386 61 1773481
Fax.: +386 61 1263126

E-mail: mcem97-at-ijs.si


_ _ _ _ _ _ _ _ _ _ _ _ _

For more information:

http://www2.ijs.si/~k5www/MCEM97/index.html





From: dianavd-at-eye.usyd.edu.au (Diana van Driel)
Date: Wed, 30 Oct 1996 10:38:27 +1100
Subject: Re: immunohistochemistry on epon blocks

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} I'm trying to get an antibody to work on glut fixed, osmicated, epon
} blocks. I have a very high background of the secondary binding to the
} sections on the copper grids, and cannot discern if the antibody is
} working at all, beyond the background "noise".

First and foremost: don't use copper grids; use nickel or gold. Only some
antibodies will still react in osmicated Epon sections - I presume you know
yours will or at least have evidence that it survives tough treatment.
There are standard methods to use: eg Ross A: Postembedding Ultrastructural
Immunocytochemistry of Neural Antigens on Tissues Previously Processed for
Routine L and EM....J EM Technique 5:81-90 (1987). Lastly, the smaller the
gold particle, the more sensitive the reaction.


Diana van Driel
Dept Ophthalmology
Sydney University C09
AUSTRALIA 2006






From: CLAYJ-at-ix.netcom.com
Date: Tue, 29 Oct 1996 16:27:00 -0800
Subject: Scleraderma

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I am looking to contact anyone doing research on sclerderma.

My appreciation is given in advance.

Clay Jordan
clayj-at-ix.netcom.com






From: Woody.N.White-at-mcdermott.com
Date: 10/28/96 3:30 PM
Subject: EDX semi-quant analysis on cubes

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Mark,

I concur with the previous posting.... The effect on the quant with
tilt will depend on the degree of tilt, the matrix, and the energy of
the x-ray of interest. Using the lowest beam potential and highest
x-ray (line) energy practical will minimize adsorption
losses/corrections.

Suggestion, if you can prepare a polished specimen of similar
composition... Using the polished material and the appropriate SEM
conditions, collect spectra at several tilt angles. Collect a similar
spectrum from the cubic specimen. The shape of the unknown (specimen)
background may then be matched to the reference backgrounds and the
associated tilt used for your calculations.

Woody
http://www.geocities.com/capecanaveral/3722


______________________________ Reply Separator _________________________________


X-To: mail11:; (-at-micro)
X-Cc: darus-at-cle.dnet.ge.com

I have a question about standardless semi-quant work that I am doing,
involving take off angle. ...SNIP...
I want to examine a powder that has cubic structures within it. ...SNIP...
of its position in space? I think the answer is yes and I've got a problem.
My problem is, how do I perform standardless semi-quant work on the
cubes within my powder, to examine homogeneity within the sample from one
cube to the next?

Thank You in advance.
Mark Darus
General Electric
Materials Characterization Laboratory
Darus-at-cle.dnet.ge.com




From: Anya Shcherbina :      shcherbina-at-cbr.med.harvard.edu
Date: Tue, 29 Oct 1996 19:32:07 -0800
Subject: EDX semi-quant analysis on cubes

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To: microscopy-at-Sparc5.Microscopy.Com


Hi!
Need advice on staining human neutrophils for cytoskeletal proteins. My
protocol works fine for other blood cells, but not for neutrophils. After
fixing in 2% paraformaldehyde and permeabilization with 0.2-0.5% Triton there
is no staining even for actin(Rh-phalloidin). I tried to use gluteraldehyde for
fixing, but I get huge autoflourescense.
Anya Shcherbina (shcherbina-at-cbr.med.harvard.edu)




From: Anthony J. Garratt-Reed :      tonygr-at-MIT.EDU
Date: Tue, 29 Oct 1996 13:25:25 -0500
Subject: Re: Pulstar

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Message-Id: {9610291825.AA22299-at-MIT.MIT.EDU}
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This is in response to Beat Frey's posting about the Pulstar pulse processor.

Beat does not say whether the application is to SEM or TEM. However, it is
important to understand what EDX maufacturers are trying to do in developing
their products.

The quality of the final analysis made with EDX depends upon the number of
x-rays detected, and the signal to background ratio in the spectrum. The
number of x-rays detected depends, of course, upon electron beam current and
the time for the analysis, but also on the solid angle the detector subtends
at the sample, and the ability of the system to process (i.e. measure) the
x-rays. The signal to background ratio is partly determined by physics, but
is improved by high energy resolution in the detector and analyzer system.
Each x-ray takes a finite time for analysis; during that time, if a second
x-ray arrives, it is lost (and, in some conditions, the first x-ray is also
lost). This leads to the familiar "Dead time" which is reported, in one way
or another, by all EDX systems. A pulse processor which processes x-rays
faster leads to a lower dead time, and therefore less loss of x-rays.
However, this is only useful if the loss of x-rays was originally
significant. If the dead time is below about 25% the loss of x-rays is
quite small.

While I'm not familiar with Noran's current product line, I would assume
from Beat's comments that the Pulstar is their newest pulse processor, which
probably is capable of a throughput of many tens of thousands of counts per
second. However, it is important to understand that this specification
assumes the x-rays will reach the detector in the first place. This leads
back to my opening comment. Are the experiments capable of generating so
many counts? If the microscope is a TEM, the samples are biological thin
sections, and the probe size small, then perhaps not. This is not to say
that upgrading to the new pulse processor would not be worthwhile otherwise,
for it would undoubtedly also give you better energy resolution at lower
count rates.

The fundamental question to be answered is the following: Do you have to
adjust your experimental conditions to get the dead time on your current
system down below 50%? (or, to rephrase, would you be able to use a larger
beam current, but at the moment you don't because the x-ray count rate would
be too high?) If the answer to either question is "yes", then the new pulse
processor would benefit you. It will also give improved energy resolution,
and will undoubtedly be serviced by the vendor for longer into the future.

My comments apply to all EDX systems, regardless of manufacturer; there may
well be other features specific to the Noran line that would be benefits to
you, too.

Hope this helps,

Tony Garratt-Reed.
*********************************
** **
** Anthony J. Garratt-Reed **
** MIT Room 13-1027 **
** 77 Massachusetts Avenue **
** Cambridge, MA 02139-4307 **
** USA **
** **
** Ph: 617-253-4622 **
** Fax: 617-258-6478 **
** **
*********************************
*********************************





From: WARRENJ1-at-cliffy.polaroid.com
Date: 10/29/96 4:01 AM
Subject: Scanner for Histological slides

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nih-image-at-soils.umn.EDU
Message-id: {01IB87WMTTK600B5QM-at-cliffy.polaroid.com}
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Content-type: TEXT/PLAIN
Content-transfer-encoding: 7BIT


We offer the SprintScan 35 that will scan 35mm slides and negatives.
There is an optional "Path Scan Enabler". This option acts as a
carrier for a glass microscope slide to be scanned by the Sprint Scan
35.

There are three models available, 10 bit/1950 dpi, 10 bit/2700 dpi,
and 12 bit/2700 dpi. They operate on both PC and Mac platforms.

The scanning software provides for a preview mode that offers image
optimization prior to the actual scan.

Please contact me if you require additional information or visit our
website at www.polaroid.com.

John D. Warren
Southern Sales Manager "see what develops"
Digital Photographic Imaging Group
Polaroid Corporation

4525 Leonard Parkway
Richmond, Virginia 23221-1809
Office 804.254.1011
Fax 804.254.1013
Internet warrenj1-at-polaroid.com








______________________________ Reply Separator _________________________________


Can someone please point me to make/model/contact info for scanners
that will digitize directly standard glass microscope slides that
contain histological sections? Thanks so much.

Marc




From: em-at-mediacity.com (Ed Monberg)
Date: Tue, 29 Oct 1996 11:42:47 -0700
Subject: Tuesday 29 October 1996 EQUIPMENT LIST from LMDC

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A Selfridge {73121.7422-at-CompuServe.COM} , aasundi-at-hkumea.hku.hk,
agitprop-at-shrine.cyber.ad.jp (Greg Dodds),
Alan Wilson {wilsona-at-blackjack.cis.dsto.gov.au} ,
Allard Mosk {mosk-at-phys.uva.nl} , Allen Graber {allen-at-homecom.com} ,
{ARRELL-at-FS-IAM-1.jrc.nl} , {art+-at-andrew.cmu.edu} Arthur W. Wetzel,
{art-at-synema.com} Art Kauffman,
{asarinan-at-campus.mty.itesm.mx} Aaro'n Sari~ana T.,
Bernie Kestel {Bernard_Kestel-at-QMGATE.ANL.COM} ,
{BI090-at-FS1-DVZ-FHKOELN.DVZ.FH-Koeln.DE} Andreas Karge,
bigguy-at-teleport.com, billg63475-at-aol.com, billp-at-ni.net,
blatt-at-pss.fit.edu, {bmval-at-anat.uct.ac.za} Boonzaier,
Bob Luffel {bobl-at-gr.hp.com} , BOB ROBERTS {ROBERTS-at-csss2.la.asu.edu} ,
brucei-at-ocumetrics.com (Bruce M. Ishimoto),
btk-at-ix.netcom.com (Bill Marriott), bxz3587-at-vaxb.isc.rit.edu,
bygrens-at-freedom.cs.TRW.COM, Calidris-at-ett.se (George Farrants),
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Charlie Lyman {cel1-at-lehigh.edu} ,
Christopher Stevenson {csteven-at-kelvin.physics.mun.ca} ,
Chuck Garber {GVKM07A-at-PRODIGY.COM} ,
Chuck Gulker {71562.1743-at-CompuServe.COM} ,
Cindy Dogan {DOGAN-at-alrc.usbm.gov} , clarke-at-acme.ist.ucf.edu,
COULSONM-at-aol.com, {craig-at-hq.sylvania.com} Craig, css-at-clark.net,
CSTOM-at-stmarytx.edu,
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davecole-at-efn.orgt.berg-at-bridge.no, DAVEL-at-cc.usu.edu,
david-at-circinus.ociw.edu, David Henriks {73531.1344-at-CompuServe.COM} ,
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{dawn_coppola-at-maca.sarnoff.com} , dbell-at-coral.bucknell.edu (David Bell),
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Ian A Paull {iap-at-vectorbd.com} , ike.song-at-anderson.ucla.edu,
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{kossover-at-tenet.edu} Marc Kossover, krish-at-mcs.com (R.Radhakrishnan),
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From: Rachel Teitelbaum :      teitelba-at-aecom.yu.edu
Date: Tue, 29 Oct 1996 10:44:41 -0500 (EST)
Subject: immunohistochemistry on epon blocks

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Rachel: While immunocytochemistry on osmicated tissues sometimes works, the
reactivity is usually less than on non-osmicated tissues; we have also had
some problems with background. I would suggest trying the following:
1. Avoid copper grids as sometimes there are reactions with reagents that can
leave a precipitate on the sections.
2. Try to oxidize the osmium before incubation with saturated sodium
metaperiodate or 10% hydrogen peroxide.
3. Try changing your blocking solution; if you are using BSA, maybe try
ovalbumin, instant milk, fish gelatin or normal serum, or a combination.
Sometimes adding Tween 20 (0.05-0.1%) will help.
4. Decrease the concentration of the primary and increase the incubation
time, e.g. overnight or longer at 4C. You might also try using tris buffer
at pH 8.2.
5. You might also increase the dilution of your secondary.

Good luck!
Art Hand
UConn Health Center
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I'm trying to get an antibody to work on glut fixed, osmicated, epon
blocks. I have a very high background of the secondary binding to the
sections on the copper grids, and cannot discern if the antibody is
working at all, beyond the background "noise". Has anyone been
successful with this, or am I wasting my time by trying to get this to
work. For my purposes, I cannot use lowicryl, or non-osmicated tissue.
Any suggestions?

Thanks,
Rachel





From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: 10/29/96 8:44 AM
Subject: determining % oxide on surface

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Mime-Version: 1.0

Hi Tanya;

Again, a little more information would be helpful, but I can think of a few
image analysis (IA) approaches if you have not already considered them.
Sounds to me like you are on the right track though, at one time we went
down the road of manual grid-counting until we zoomed into the '90's and
splurged on IA software.

1) If the oxide is fully distinguishable from the unoxidized surface by
some topographical means or contrast difference using light microscopy or
SEM, there are a number of IA programs that could simplify this assay. We
have performed similar analyses using a PC version of program called
"Imagemeasure", version 4.02, by Phoenix Technology, Inc. This software is
sold in modules (counting & sizing, morphometry, densitometry, etc.)
depending upon your needs. We used the counting and sizing module to
determine the percentage of damage to corneal endothelial cell surfaces
when touched by a plastic implant.

2) Other responses have suggested using backscatter detection, and this is
another possibility. Obviously, in this procedure the unoxidized surfaces
will appear brighter and can be quantitated using an IA program similar to
that mentioned above.

Disclaimer: No interest in Phoenix Technology, commercial or otherwise,
but if you would like their address or phone # contact me directly.

Good luck with your assay; hope this is useful.

Regards,

-Bob
**************************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA 91711
ph: (909)399-1311
email: Bob_Citron-at-cc.chiron.com
**************************************


______________________________ Reply Separator _________________________________


Thank you for your responses, but I fear I may have posed the wrong
question. I am looking for oxide on the surface in more of an image
analysis point-of-view, than quantifying the thickness of the oxide.
I am considering the oxide to be like paint, and want to find the
percentage of the wire's surface uncovered by the "paint". Typically,
we have taken several photographs of the surface and crudely calculate
the surface covered in oxide.

I hope I've explained myself a little better this time.

Thanks in advance,

Tania Jones

tania-at-dynamotive.com





From: Ray_Ortiz_at_RLT011-at-ccmailgw.mcgawpark.baxter.com
Date: 10/29/96 8:01 AM
Subject: Scanner for Histological slides

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"Marc C. Brande; MS; Founder" {mcbrande-at-sierra.net}
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Hi Marc,

I use the polaroid SprintScan 35 when I am scanning histological
sections. I use with it the PathScan enabler. The SprintScan can be
used with either Windows or Macintosh with the appropiate software and
SCSI. You can get the Scanner at a computer store. The PathScan
enabler is available from Meyer Instruments (713)579-0342, Houston
Texas.

The scanner gives pretty good resolution from the entire slide. The
files tend to be quite large. So you want to make sure you have
enough RAM and space to store these files.


______________________________ Reply Separator _________________________________


Can someone please point me to make/model/contact info for scanners
that will digitize directly standard glass microscope slides that
contain histological sections? Thanks so much.

Marc

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From: Ray_Ortiz_at_RLT011-at-ccmailgw.mcgawpark.baxter.com
Date: 10/29/96 8:01 AM
Subject: Scanner for Histological slides

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From: Marc C. Brande, MS, Founder :      mcbrande-at-sierra.net
Date: Tue, 29 Oct 1996 08:01:18 +0000
Subject: Scanner for Histological slides

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From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Wed, 30 Oct 1996 07:41:12 -0500
Subject: EM@Mediacity.com has been Unsubscribed

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Colleagues

You will have received an Email "sales" bulletin from
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From: kna101-at-utdallas.edu
Date: Wed, 30 Oct 1996 08:46:32 -0600 (CST)
Subject: Re: immunohistochemistry on epon blocks

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glenmac-at-u.washington.edu, microscopy-at-Sparc5.Microscopy.Com

Rachel,

It's been a while since I did immunohistochemistry at the EM
level, but when I was doing it I had a problem with high background stain
that I was able to reduce by using nickel instead of copper grids. It
had something to do with an interaction between the copper and the
colloidal gold I think... I have never tried osmium fixed tissue,
however, so I can't help with that part.

Karen

On Tue, 29 Oct 1996, Rachel Teitelbaum wrote:

}
} I'm trying to get an antibody to work on glut fixed, osmicated, epon
} blocks. I have a very high background of the secondary binding to the
} sections on the copper grids, and cannot discern if the antibody is
} working at all, beyond the background "noise". Has anyone been
} successful with this, or am I wasting my time by trying to get this to
} work. For my purposes, I cannot use lowicryl, or non-osmicated tissue.
} Any suggestions?
}
} Thanks,
} Rachel
}




From: leah dobbs :      leadob-at-execpc.com
Date: Wed, 30 Oct 1996 09:33:23 -0600 (CST)
Subject: ultramicrotomy of paper

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I will be doing ultramicrotomy of paper which has been coated. Could I
please have suggestions and comments on an embedment process.

Thank you

Leah L. Dobbs
Madison Area Technical College
E-mail: leadob-at-execpc.com or ldobbs-at-madison.tec.wi.us





From: dlc-at-rice.edu (Daniel L. Callahan)
Date: Wed, 30 Oct 1996 10:29:43 -0600
Subject: metallography / FA course thoughts...

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Message-Id: {199610301629.KAA13417-at-owlnet.rice.edu}

Hello...

I'm putting together a course in metallography and failure analysis for a
mixed senior undergraduate / graduate group. I would like to raise the
intellectual content of the course beyond the typical technique survey and
review of case studies and add a significant component of materials
reliability and electrical/electronic FA. Any thoughts or suggestions
would be appreciated.

Does anyone share my concerns about intellectual content of FA-type courses
or have a positive example (outside of a short-course)? Several
universities seem to combine FA with design mechanics to add some
analytical content, but I would prefer to stay closer to materials failures
while broadening the industrial scope. The incorporation of tribological
failures (e.g. magnetic media) and electronic failures (electromigration,
dielectric breakdown) seems to be woefully lacking in texts on the matter.

Thanks
Daniel L. Callahan
Assistant Professor
Mechanical Engineering and Materials Science
Rice University

dlc-at-rice.edu
(713) 527-8101 x3572
http://www.owlnet.rice.edu:80/~dlc/





From: Jaime Finguerut :      JAIME-at-azul.ctc.com.br
Date: Wed, 30 Oct 1996 15:07:04 GMT+3
Subject: Automated, on-line microscopy

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nih-image-at-soils.umn.edu

Dear colleagues:

We work with ethanol production by alcoholic fermentation in large
industrial plants.
One of the main controls is the microscopic examination of the yeasts
in the several stages of the process.
In order to improve this control we would like very much to develop
or to buy a system to do the examination automatically.
Is there such a system ?
I mean, a system that resembles a Flow Injection Analysis, i.e. it
takes a sample from the fermenter, dilute it, mix with the contrast
inject it at the microscope slide (continuously?) take a sample image
and analyse it.
Any hint about it, mainly a contact person, would be very, very nice.
I thank you in anticipation for your kind attention to our problems.

Best regards





Jaime Finguerut(Mr., chem.eng.)
Cx.Postal 162 Piracicaba
Sao Paulo BRAZIL 13400-970
fax 0055 194 29 8388




From: Lesley S. Smith :      lesleys-at-pobox.upenn.edu
Date: Wed, 30 Oct 1996 13:32:48 -0500 (EST)
Subject: Epon Blocks-thanks!

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To everyone who gave me tips on removing epon blocks from their molds - I
want to thank you. I knew microscopists were creative but I didn't
realise just HOW creative everyone was!

Lesley Bechtold
lesleys-at-pobox.upenn.edu




From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Wed, 30 Oct 1996 13:04:03 -0700
Subject: Re: ImageSlave

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This reply comes from the US distributor of ImageSlave. His address is jhilton-at-rmi.net.

} Dear MicroPeople: Reply to ImageSlave under NT.
}
} It will not work, since the HAL spec. is not met, the card is not PCi, the
} software is not 32bit. However, it might work in NT using the DOS engine.
} But what's the point?
}
} Jim Hilton
}
} } Jim, this just came through the microscopy listserv. Thought I'd pass it
} along, in case you don't subscribe.
} }
} } } Date: Tue, 29 Oct 1996 09:06:43 +0800
} } } From: SCM!ATitkov-at-scmaust.attmail.com (SCM!ATitkov)
} } } Subject: ImageSlave
} } } To: Microscopy-at-Sparc5.Microscopy.Com
} } } Mime-Version: 1.0
} } } Status:
} } }
} } } Dear Microscopists,
} } }
} } } Has anyone tried to use ImageSlave under Windows NT?
} } }
} } } Alexander Titkov
} } } SCM Chemicals Ltd.
} } } PO Box 245
} } } Bunbury WA 6231
} } } Australia
} } } Ph (097) 808 505

John
chandler-at-lamar.ColoState.EDU






From: A. Kent Christensen :      akc-at-umich.edu
Date: Wed, 30 Oct 1996 08:38:26 -0500 (EST)
Subject: Re: immunohistochemistry on epon blocks

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Another reference that may be of interest is: Bendayan M, Zollinger M,
1983, "Ultrastructural localization of antigenic sites in osmium-fixed
tissues applying the protein A-gold technique", J Histochem Cytochem
31:101-109.

A. Kent Christensen
University of Michigan
{akc-at-umich.edu}

---------------------------------

On Wed, 30 Oct 1996, Diana van Driel wrote:

} } I'm trying to get an antibody to work on glut fixed, osmicated, epon
} } blocks. I have a very high background of the secondary binding to the
} } sections on the copper grids, and cannot discern if the antibody is
} } working at all, beyond the background "noise".
}
} First and foremost: don't use copper grids; use nickel or gold. Only some
} antibodies will still react in osmicated Epon sections - I presume you know
} yours will or at least have evidence that it survives tough treatment.
} There are standard methods to use: eg Ross A: Postembedding Ultrastructural
} Immunocytochemistry of Neural Antigens on Tissues Previously Processed for
} Routine L and EM....J EM Technique 5:81-90 (1987). Lastly, the smaller the
} gold particle, the more sensitive the reaction.
}
}
} Diana van Driel
} Dept Ophthalmology
} Sydney University C09
} AUSTRALIA 2006
}
}
}





From: Anya Shcherbina :      shcherbina-at-cbr.med.harvard.edu
Date: Wed, 30 Oct 1996 16:12:02 -0800
Subject: Re: immunohistochemistry on epon blocks

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X-Mailer: BeyondMail for Windows/SMTP 2.2
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To: Microscopy-at-Sparc5.Microscopy.Com

Hi!
Need advice on staining human neutrophils for cytoskeletal proteins. My
protocol works fine for other blood cells, but not for neutrophils.
After
fixing in 2% paraformaldehyde and permeabilization with 0.2-0.5% Triton
there
is no staining even for actin(Rh-phalloidin). I tried to use
gluteraldehyde
for
fixing, but I get huge autofluorescense.
Anya Shcherbina




From: Microscopy-request
Date: Wednesday, October 30, 1996 9:33AM
Subject: ultramicrotomy of paper

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I will be doing ultramicrotomy of paper which has been coated. Could I
please have suggestions and comments on an embedment process.

Thank you

Leah L. Dobbs
Madison Area Technical College
E-mail: leadob-at-execpc.com or ldobbs-at-madison.tec.wi.us





From: James Drummond :      drummond_james-at-VANLAB.PAPRICAN.CA
Date: Wed, 30 Oct 1996 11:29:23 PDT
Subject: Re: ultramicrotomy of paper

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Message-Id: {199610301516.KAA12374-at-ns1.axs2000.net}
To: MICROSCOPY BB {Microscopy-at-Sparc5.Microscopy.Com}

} I will be doing ultramicrotomy of paper which has been coated.
Could I
} please have suggestions and comments on an embedment
process.
}
} Thank you
}
} Leah L. Dobbs
}

Coated paper can be tricky. We've used Spurr's resin successfully -
make sure the edges of the sample are clean (ie cut with fresh razor
blade or scalpel) since these edges will be the route for the resin to
pentrate. Long infiltration times, use of a rotator, and alternating
vacuum to atmosphere cycling can also help (but avoid excessive
time under vacuum and use appropriate safety measures).

There are some references available in the pulp and paper industry
literature. You might want to search out articles by Dale
Quackenbush - he used epon812 for embedding and cut high quality 2
micron thick sections with a sledge microtome! Most of his work is
found in TAPPI magazine or The Microscope.

I hope this is a good starting point.

James Drummond
Pulp and Paper Research Institute of Canada
3800 Wesbrook Mall, Vancouver B.C., V6S 2L9
Phone: (604)222-3200
Fax: (604)222-3207




From: Hyejung Choi :      chj-at-eden.rutgers.edu
Date: Wed, 30 Oct 1996 23:21:37 -0500
Subject: LM-Polar Lipids Staining?

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Is there any staining method that can differentiate polar lipids
(e.g. mono, diglyceride etc.) from neutral lipids?

Thanks in advance.

Hyejung Choi
Dept. of Food Science
Rugers University
New Brunswick, NJ
e-mail) chj-at-eden.rutgers.edu




From: Hyejung Choi :      chj-at-eden.rutgers.edu
Date: Wed, 30 Oct 1996 23:21:37 -0500
Subject: LM-Polar Lipids Staining?

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Is there any staining method that can differentiate polar lipids
(e.g. mono, diglyceride etc.) from neutral lipids?

Thanks in advance.

Hyejung Choi
Dept. of Food Science
Rugers University
New Brunswick, NJ
e-mail) chj-at-eden.rutgers.edu




From: Margareta Halin :      Margareta.Halin-at-ah.slu.se,Margareta.Halin-at-medicin.uu.se
Date: Thu, 31 Oct 1996 09:19:06 +0100
Subject: Immunocytochemistry/osmicated tissue

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I never use anything but Ni-grids -when I in the beginning happened to
incubate the primary ab overnight and used coppergrids, the drop turned
blue, so something definitely goes wrong there...

Like everybody else already said, there are antibodies that work with
osmicated tissue, I have never tried it though. What I would like to add, is
that I a couple of years ago I saw a paper from a japanese (?) group, that
had reduced their osmium with potassiumferrocyanide, and that made their ICC
work better.
Maybe you can find that article? (I didn't keep it.)

Margareta





From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Thu, 31 Oct 1996 08:23:20 +0000
Subject: Re: determining % oxide on surface

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} Thank you for your responses, but I fear I may have posed the wrong
} question. I am looking for oxide on the surface in more of an image
} analysis point-of-view, than quantifying the thickness of the oxide.
} I am considering the oxide to be like paint, and want to find the
} percentage of the wire's surface uncovered by the "paint". Typically,
} we have taken several photographs of the surface and crudely calculate
} the surface covered in oxide.
}
} I hope I've explained myself a little better this time.
}
} Thanks in advance,
}
} Tania Jones
}
} tania-at-dynamotive.com

Any 'automated' approach will rely on having something in the image that
clearly distinguishes the oxide from the rest of the specimen. If you have
that then most image analysis programmes will handle the problem within
seconds.

If you have a 'modern' SEM, you can probably transfer the image directly
into your image analysis computer. With older SEMs, you'll either have to
connect your computer to the SEM via a frame grabber, have an image
scanned.

As to regards software, I'd suggest starting with free/shareware - NIH
Image is a good option. This will give you a chance to evaluate the
procedure without committing too much money.

Regards,
Larry Stoter






From: Walt Bobrowski (313) 996-7814 :      bobroww-at-aa.wl.com
Date: Thu, 31 Oct 1996 09:50:16 -0500 (EST)
Subject: Re: Reduced Osmium Immunocytochemistry

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Mr-Received: by mta SRVR05.MUAS; Relayed; Thu, 31 Oct 1996 09:50:16 -0500
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One paper is entitled, "Improved Method for Post-embedding Cytochemistry Using
Reduced Osmium and LR White Resin", H. Tamaki and S. Yamashina, J.
Histochemistry and Cytochemistry, 42:1285-1293, 1994.

Best regards,
Walt Bobrowski
Parke-Davis Research

} I never use anything but Ni-grids -when I in the beginning happened to
} incubate the primary ab overnight and used coppergrids, the drop turned
} blue, so something definitely goes wrong there...
}
} Like everybody else already said, there are antibodies that work with
} osmicated tissue, I have never tried it though. What I would like to add, is
} that I a couple of years ago I saw a paper from a japanese (?) group, that
} had reduced their osmium with potassiumferrocyanide, and that made their ICC
} work better.
} Maybe you can find that article? (I didn't keep it.)
}
} Margareta





From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Thu, 31 Oct 1996 15:04:41 -500
Subject: Re: Automated, on-line microscopy

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Not knowing exactly what yeast cell parameters your interested in
it would seem to me that an intergration of a flowcytometery system
and a machine vision / auto-recognition system might be a way of
acheiving what you need. Since you are involved in industrial
fermentation a bigger concern would be the wide location of
fermentation vats and an analysis system. (.... allowing my
imagingation to run wild: the possibility of floor track guided
automatic sample withdrawal system collecting and transfering samples
from vat to analysis workstation....mmm)



Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu




From: Daryl.Davis-at-anlw.anl.gov (Daryl Davis)
Date: Thu, 31 Oct 1996 14:48:35 -0700
Subject: Beem mold users

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For anyone who has cut their fingers using beem molds and razor blades
there is a new development called "easy molds". The epoxy bullets and
samples remove easily so a razor blade is not necessary to remove the
samples. The person to contact regarding these new molds is:

Mike Boykin at
boykin-at-mindspring.com




From: Marc C. Brande, MS, Founder :      mcbrande-at-sierra.net
Date: Thu, 31 Oct 1996 14:27:20 +0000
Subject: How to Convert DPI to Pixels?

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Message-Id: {3278B748.260A-at-sierra.net}

Would someone kindly explain how to convert dpi (dots-per-inch) to
pixels?

I would like to evaluate scanner resolution (ie. 2700 dpi) in terms of
pixels.

Thanks for any help on this topic.

Marc




From: Beverly E Maleeff
Date: 31 Oct 96 17:09:38 EDT
Subject: PSM November '96 Meeting Notice

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Message-Id: {9611010108.AA1308-at-pho903.sbphrd.com}
To: microscopy {microscopy-at-Sparc5.Microscopy.Com}
{Beverly_E_Maleeff-at-sbphrd.com}

PHILADELPHIA SOCIETY FOR MICROSCOPY

NOVEMBER 1996 MEETING NOTICE

Wednesday, November 13, 1996

New Frontiers in Thoracic Imaging

Warren B. Gefter, M.D.
Department of Radiology
University of Pennsylvania Medical Center
Philadelphia, PA

This presentation traces the rapid evolution of the following exciting advances
currently taking place in thoracic imaging: digital (computed) radiography,
progress in computed tomography (CT) including high-resolution CT, helical and
electron beam (ultrafast) CT; magnetic resonance imaging (MRI) and volumetric
image display methods such as virtual endoscopy. These methods are providing
multidimensional displays of cardiopulmonary anatomy and physiology. Examples
of the imaging provided by these techniques will be shown. In addition,
ongoing developments in volumetric image processing techniques are providing
methods to display these complex multidimensional datasets, useful in both the
clinical and research realms.



DATE: Wednesday, November 13, 1996

PLACE: Laboratory for the Research of Science and Materials
(LRSM) Building, 33rd and Walnut Street (map enclosed).
Parking is available behind the LRSM Building after 5:00 PM.

TIME: 5:30 PM Social hour, hosted by our meeting sponsors

6:30 PM Dinner
Members $12.00 Student members $6.00 Non-members $15.00

Menu: Beer, wine, assorted soda and bottled water
Munchies

Baked ziti with ricotta, mozzarella and parmagiana cheeses
Breast of chicken
House salad with Italian dressing
Chef's vegetable du jour
Rolls and butter

Golden cake with chocolate icing

Coffee, decaf or tea


7:30 PM Speaker



Reservations will be taken by Ms. Pat Overend at the University of
Pennsylvania, 215/898-8337.
Deadline for reservations will be Monday, November 11. If you have any
questions regarding the
meeting please feel free to contact Rollin Lakis of the Executive Council.
Cancellations must be
received by Ms. Overend no later than 5:00 PM, November 11, 1996.

RESERVATIONS ARE REQUIRED FOR DINNER. We cannot guarantee you a meal if you
do not make a reservation by the above deadline.










From: Larry Dodge :      ldodge-at-eznet.net
Date: Thu, 31 Oct 1996 19:47:54 -0500
Subject: Macintosh Image Databases

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Message-ID: {327948A3.7D40-at-eznet.net}

I am looking for an image database for the Apple Macintosh. Does any
one know of a good imaage database for the macintosh?




From: shaf :      mshaf-at-darkwing.uoregon.edu
Date: Thu, 31 Oct 1996 17:40:14 -0300
Subject: Re: How to Convert DPI to Pixels?

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Message-Id: {2.2.32.19961031204014.0069e4cc-at-darkwing.uoregon.edu}
X-Sender: mshaf-at-darkwing.uoregon.edu
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Mime-Version: 1.0
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At 02:27 PM 10/31/96 +0000, Marc wrote:
}
} Would someone kindly explain how to convert dpi (dots-per-inch) to
} pixels?
}
} I would like to evaluate scanner resolution (ie. 2700 dpi) in terms of
} pixels.
}
"Dots" (as in dots per inch) are usually equated to pixels ... that is, if
you scan a 35mm slide (1.25"" by 1") at 2700dpi you'll end up with
approximately a 3400 by 2700 pixel image. If this is then printed with a
300dpi printer then its final size would be (approximately) 11" by 9".

The confusion generally sets in when some printers demand you are
knowledgeable of what it is capable of printing in terms of "lines per inch"
(lpi), which has much to do with its inability of printing one of the 16M
colors as one "dot" (dye-sub printers have this ability), but instead need a
small matrix of dots to dither CMYK into a resemblance of one of 16M colors.
Thus, if a 300dpi printer (ink jets, color laser) needs an 8by8 matrix to
create one of these colors then its true capability is 300/8 or ~37 lines
per inch.

So much for the math ... there is a lot of gray area here such as
"apparent" capabilities for printers to do better than this in spite of the
math ... so begin with an understanding of the terms and experiment ...

hope this helps ...

cheers, shaf
{/} \ {/} \ {/} \ {/} \ {/} \ {/} cognito, ergo zZOooOM {/} \ {/} \ {/} \ {/} \ {/} \ {/}
Michael Shaffer - mshaf-at-oregon.uoregon.edu - mshaf-at-darkwing.uoregon.edu
Electron Microprobe Facility - Geological Sciences - University of Oregon
http://darkwing.uoregon.edu/~mshaf/shafhome/






From: s002swh-at-desire.wright.edu
Date: Thu, 31 Oct 1996 21:32:12 -0500 (EST)
Subject: Re: How to Convert DPI to Pixels?

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unsubscribe





From: Young Woo Jeong :      jyw-at-wm.lge.co.kr
Date: Fri, 01 Nov 1996 13:45:44 +0900
Subject: re-subscribe

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Message-ID: {32798077.2149-at-wm.lge.co.kr}

Hello,
My E-Mail address is changed to jyw-at-wm.lge.co.kr

Old E-mail address : jyw-at-gscrl.goldstar.co.kr
New E-mail address : jyw-at-wm.lge.co.kr




From: csbeneas :      csbeneas-at-wiccmail.weizmann.ac.il
Date: 1 Nov 1996 12:02:28 +0200
Subject: inverted microscope

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Message-ID: {n1365272706.9629-at-wiccmail.weizmann.ac.il}

we are lookng for an inverted microscope, something simple and cheap mb second
hand, if you have any suggestions and offers, please send them directly to my
address csbeneas-at-wiccmail.weizmann.ac.il, thank you in advance, Elia (-:




From: Daryl Davis
Date: 01 November 1996 02:42
Subject: Beem mold users

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Another alternative to avoid cutting your fingers when opening BEEM capsules
with a razor blade is simply to clamp the BEEM capsule in a small bench top
vice, cut one side of the capsule, turn it over and cut the other side - it
takes only seconds. You can buy a small removable vice for under 20 UK
pounds (shop around) which can simply be clamped to any bench (including the
microtome) and of course it has 1001 uses unlike a BEEM splitter.

Malcolm Haswell
University of Sunderland
UK
----------


For anyone who has cut their fingers using beem molds and razor blades
there is a new development called "easy molds". The epoxy bullets and
samples remove easily so a razor blade is not necessary to remove the
samples. The person to contact regarding these new molds is:

Mike Boykin at
boykin-at-mindspring.com





From: Tom Phillips :      tphillips-at-biosci.mbp.missouri.edu
Date: Fri, 1 Nov 1996 09:26:51 -0500
Subject: counter staining clatharin in EM

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I have looked in the literature without much success for a
non-immunological method to stained clatharin coated vesicles. I seem to
recall somebody using bismuth? to enhance the coat staining. I have found
some references to pre-embedding staining with tannic acid but I would like
to use existing blocks of formaldehyde fixed, LR Gold embedded tissue. I
have nice colloidal gold labeled staining of some primary antibodies and
would now like to prove they are in coated vesicles sometimes. TIA.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(573)-882-4712 (voice)
(573)-882-0123 (fax)






From: jacobb-at-mh1.lbl.gov (Jacob Bastacky)
Date: Fri, 1 Nov 1996 08:16:21 -0800
Subject: Wanted: Balzers freeze fracture machine

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We are looking for a donation of a Balzers freeze fracture machine,
preferably 300 series, that we could use for double-layer coating
(platinum/carbon) for low-temperature SEM. A conventional machine would
do. This would be for the University of California, Berkeley.
Please reply if you know of anyone with one of these machines; any leads
would help.
Thanks,
Jacob Bastacky

Jacob Bastacky
Room 116 Donner Laboratory
Lawrence Berkeley Laboratory EMail: sjbastacky-at-lbl.gov
University of California Phone: (510) 486-4606
Berkeley, California 94720 Fax: (510) 486-4750






From: cvierret-at-misn.com
Date: Fri, 1 Nov 1996 12:08:19 -0500 (EST)
Subject: Lead Staining

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I just recieved my EMS catalog in the mail. In this volumn it has Techinical
Tips. The first tip deals with "A Stable Lead Staining Solution". It is used
to increase contrast and reduc contamination in secions for EM. Could some one
share a more detailed description of its uses and what it should be used on?
Thank you, Clarissa

Clarissa Vierrether
The Doe Run Co.
PO Box 500
Viburnum, MO 65566
cvierret-at-misn.com




From: Glen MacDonald :      glenmac-at-franklin01.u.washington.edu
Date: Fri, 01 Nov 1996 10:46:36 -0800
Subject: Re: Mac Security

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The experiences of our school district led them to abandon At Ease in favor of
FoolProof. I installed it on many machines as a volunteer and found it much
more robust and conflict-free than AT Ease. I can't recall the company, but
they are in Oregon.

Glen MacDonald
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu





From: wise-at-vaxa.cis.uwosh.edu
Date: Fri, 01 Nov 1996 14:51:42 +0000
Subject: Re: Lead Staining

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} I just recieved my EMS catalog in the mail. In this volumn it has Techinical
} Tips. The first tip deals with "A Stable Lead Staining Solution". It is used
} to increase contrast and reduc contamination in secions for EM. Could some
} one
} share a more detailed description of its uses and what it should be used on?
} Thank you, Clarissa
}
} Clarissa Vierrether
} The Doe Run Co.
} PO Box 500
} Viburnum, MO 65566
} cvierret-at-misn.com


I switched to the Haniachi et al. calcined lead solution about two years
ago and really like it. A solution made up and stored in plastic has
lasted over a year at room temperature. Contamination is very low
(although high contamination rates are possible-just ask some of my
students), contrast is very nice, and staining times are short (~2 min). I
double stain with 30-60 min of UrAc (saturated in 70% ethanol). I work
with plant tissue--mostly leaves--and they typicaly have pretty low
contrast. I'm very pleased with the calcined lead recipe. I've never been
sure just how long to heat the lead citrate ("heat for several hours at
200-300C until it turns a light yellow") but I've never failed to make a
good batch of stain.

Bob







From: Warren Straszheim :      wes-at-ameslab.gov
Date: Fri, 01 Nov 1996 08:22:20 -0600
Subject: Re: How to Convert DPI to Pixels?

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Under 'normal' conditions one scan dot is equal to one pixel of an image.
When we scan photographic images for reports or the web, we are usually not
pushing the limits of our scanner by any means. If we aim to convert a 4x5
inch photo to a 800x1000 pixel image, then all we need is 200 dpi (pixels
per inch) resolution. Even cheap scanners give that, and such an image is
big for the web. A 1600x2000 pixel image would require 400 dpi which is
still within the capability of most scanners. However, to scan a 35mm
transparency to the same image size would require about 4 times the dpi
capability as the 4x5 Polaroid. Therefore, the new scanner capabilities will
be needed.

There are also the phrases "optical resolution" and "interpolated
resolution". I understand optical resolution to be the actual spacing of the
elements of the scanning mechanism. Interpolated resolution refers to extra
resolution obtained by mathematical (or mechanical?) tricks to see in
between the pixels. It may help, but I would always check the optical
resolution as the better measure of capability.

Now on printing, laser printers will normally need more dots per inch than
you are printing pixels per inch. The reason is that the printer pixels/dots
are only black or white and are dithered together to generate an area of
apparent gray. For example a 3x3 or larger printer area will be needed to
give the impression of shades of gray for a single image pixel. I think that
600 dpi printers are the practical *minimum* for printing gray scale images.
1200 dpi printers are better.

True gray scale printers (e.g., most dye subs) require less dpi to give a
similar quality image than does a laser printer. Of course they will tend to
be more expensive.

Hope this helps.

At 02:27 PM 10/31/96 +0000, you wrote:
} Would someone kindly explain how to convert dpi (dots-per-inch) to
} pixels?
}
} I would like to evaluate scanner resolution (ie. 2700 dpi) in terms of
} pixels.
}
} Thanks for any help on this topic.
}
} Marc
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: nzjaba-at-madison.tec.wi.us
Date: Fri, 1 Nov 1996 15:39:54 -0600
Subject: Thanks, re, EM in forensics

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Thanks to all who responded to my inquiry regarding the
use of EM in forensics. Your responses and leads
were very helpful.

nz

********************************************************************
* *
* Nancy E. Zjaba, student *
* Madison Area Technical College *
* Electron Microscopy Department *
* Home address: *
* P.O. Box 669 *
* Madison, WI 53701-0669 *
* (608) 251-5719 *
* email: nzjaba-at-madison.tec.wi.us *
* or, zjaba-at-engr.wisc.edu *
********************************************************************







From: reffner :      102223.406-at-CompuServe.COM (by way of Nestor J. Zaluzec)
Date: Fri, 1 Nov 1996 17:26:41 -0500
Subject: Autofocus for light microscopy

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I keep hearing that autofocus is a desired feature on light microscopes. Is
this really true or am I just an old fashioned knob turner.

I would like to learn about your hands-on experiance with autofocus. Who are
the major providers of autofocus? Who is the best supplier? What are the
major applications for autofocus?

Thanks for your help.

John A. Reffner

102223.406-at-compuserve.com






From: becks-at-sunynassau.edu (Steve Beck)
Date: Fri, 1 Nov 1996 19:16:41 -0500
Subject: Videoprinter

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Greetings All!

I have inherited a videoprinter and would like to obtain some information
on it. A metal plate on the front panel identifies it as an AXIOM, TX-500
Videoprinter. A metal plate on the rear panel indicates that it is
manufactured by Seiko/Seikosha and the reference VP-35.

Does anyone have any information on this printer, its quality, resolution,
etc. In addition, does anyone know of a source of paper for this model - it
appears to use paper which is approximately 5" wide. It also requires a
power cord, however, it appears to be a standard 3-prong computer/hard
drive cord which is readily obtainable.

Thanks in advance!


Stephen J. Beck
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}






From: Dr. Mark W. Lund :      lundm-at-physc2.byu.edu
Date: Fri, 01 Nov 1996 16:32:06 MST/MDT
Subject: Re: Pulstar

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Sender: lundm-at-physc1.byu.edu


} From: "Anthony J. Garratt-Reed" {tonygr-at-MIT.EDU}
} Subject: Re: Pulstar

This is in response to Antony Garrat-Reed's response to
Beat Frey's posting about the Pulstar pulse processor.

} The quality of the final analysis made with EDX depends upon the number of
} x-rays detected, and the signal to background ratio in the spectrum. The
} number of x-rays detected depends, of course, upon electron beam current and
} the time for the analysis, but also on the solid angle the detector subtends
} at the sample, and the ability of the system to process (i.e. measure) the
} x-rays. The signal to background ratio is partly determined by physics,
} but is improved by high energy resolution in the detector and analyzer system.
} Each x-ray takes a finite time for analysis; during that time, if a second
} x-ray arrives, it is lost (and, in some conditions, the first x-ray is also
} lost). This leads to the familiar "Dead time" which is reported, in one way
} or another, by all EDX systems. A pulse processor which processes x-rays
} faster leads to a lower dead time, and therefore less loss of x-rays.
} However, this is only useful if the loss of x-rays was originally
} significant. If the dead time is below about 25% the loss of x-rays is
} quite small.

A bit is missing from this--longer processing times give better resolution.
Since a digital pulse processor uses all the time available between pulses
the resolution is better for any given countrate than a comparable analog
processor runing at the same countrate.

} ...... This is not to say
} that upgrading to the new pulse processor would not be worthwhile otherwise,
} for it would undoubtedly also give you better energy resolution at lower
} count rates.

This of course implies what I added.

Best regards
mark

Mark W. Lund, PhD
Director } } Soft X-ray Web page http://www.moxtek.com { {
MOXTEK, Inc. *************************************************
Orem UT 84057 **"Soft x-rays in the 21st Century" conference **
801-225-0930 ** 8-11 January 1997, Midway Utah **
FAX 801-221-1121 ** http://volta.byu.edu/xray/info.html **
lundm-at-xray.byu.edu *************************************************

"Let me commend a great truth to you which has been one of the supports
of my life: 'The Gods send threads for a web begun.' Andrew Carnegie




From: David Henry Collins :      dhcollin-at-eos.ncsu.edu
Date: Sat, 02 Nov 1996 10:40:19 -0500
Subject: Immersion Lens SEM

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Sender: dhcollin-at-eos.ncsu.edu
Message-ID: {327B6B63.52D0-at-eos.ncsu.edu}

I am writing a research paper on Immersion Lens SEM for a class, and as
part of my thesis, and I have found very little articles or Journals by
searching, does anyone know of where I can find more info? Thanks
--
David Collins




From: John Best :      jbest-at-vicon.net
Date: Thu, 31 Oct 1996 17:35:34 -0800
Subject: Re: BS detectors

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Message-ID: {327953E6.C21-at-vicon.net}
"microscopy-at-Sparc5.Microscopy.Co" {microscopy-at-Sparc5.Microscopy.Com}

Jake,

Glad to get your response. I was unaware that micro-chanell plates has a
topographic mode. Thanks.

John.


Jake Schaper wrote:
}
} RE} } BS detectors 10/29/96
} I have a microchannel plate on my 6300F JEOL. It performs extremely well
in both modes - compositional (A+B) and topographical (A-B), and in low
KV applications. It was purchased in 1995. If purchasing a new detector
it is well worth evaluating this type, I'm sure there is more than one
manufacturer.
The one I have was built by Galileo Electro-Optics Corp. in Mass.
(800-648-1800).
No interest in the company.....just providing info.





From: Corvos-at-aol.com
Date: Sun, 3 Nov 1996 11:46:32 -0500
Subject: Electron Microprobe

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Message-Id: {9611022311.AA23635-at-ceam.UCSD.EDU}

All,

There is possibility of an electron microprobe comming availble in the next 5
months. If you are intrested, I will be keeping a list for the customer to
look at.....

I would like to know if their is a possible home for the following
instrument:
Cameca Electron Microprobe - MBX version - ~1984
3 wavelength spectrometers
Kevex EDS
Cathodoluminescence Detector
BSE
DEC computer

System will have to be used and not given as scrap..
Possible qualifications could include either DOD or DOE contracts...

Installation, repairs, upgrades and operating expences for first year could
be as high as $100K...

Only serious inquires apply....
I will not be involved in the selection of recipent....

Thank you,

Walter Protheror
E-MAC





From: Corvos-at-aol.com
Date: Sun, 3 Nov 1996 11:46:32 -0500
Subject: Electron Microprobe

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All,

There is possibility of an electron microprobe comming availble in the next 5
months. If you are intrested, I will be keeping a list for the customer to
look at.....

I would like to know if their is a possible home for the following
instrument:
Cameca Electron Microprobe - MBX version - ~1984
3 wavelength spectrometers
Kevex EDS
Cathodoluminescence Detector
BSE
DEC computer

System will have to be used and not given as scrap..
Possible qualifications could include either DOD or DOE contracts...

Installation, repairs, upgrades and operating expences for first year could
be as high as $100K...

Only serious inquires apply....
I will not be involved in the selection of recipent....

Thank you,

Walter Protheror
E-MAC





From: Ann FookYang (Ann-Fook Yang) :      YANGA-at-em.agr.ca
Date: Mon, 04 Nov 1996 09:53:57 -0500
Subject: Fried filament

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X-Mailer: Novell GroupWise 4.1

Hi, everyone,

I have a Zeiss 940 SEM. A tungsten filament that can last for 200hr. is
now fried in about 10 hr. A chunk o filament tip disappeared; the broken
ends were thinned out and pointed like needle. A lot of black deposits
were found inside and outside of the whenelt aperture disc and on the
anode.

Normally, I leave the saturation point untouched at the end of operation.
On the next session, when Vacuum Ready light is lit, I push HT and
Filament buttons, reset saturation point and off I go. It is equipped with
a turbo pump, therefore, HT and filament must be turned off before
specimen change and turned on again when the vacuum is ready. The
filament would be turned on and off several times a day.

I suspected a leak at the gun chamber but the vacuum remains stable
when I squirted methanol at the gap.

What is the cause of this problem? Any idea?

Thanks.

Ann Fook Yang




From: John G. Aghajanian, Ph.D. :      JOHNA-at-SCI.WFBR.EDU
Date: Mon, 04 Nov 1996 11:29:28 -0800
Subject: Beem capsule removal

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Hello folks,

I haven't used a razor blade on beem capsules in years. I just used a
regular old pair of pliers. I gently squeeze around the capsule and
watch it separate from the block, then squeeze the bottom (pyramidal)
end until it separates. By separating I mean that you can see that the
beem capsule and the epon are not adherent; now there's a layer of air
between the two. Then you just carefully squeeze the bottom of the
capsule just where the pyramid begins and the block either slides out or
pops out depending on how hard you squeeze. If you squeeze too hard or
have not completely separated the capsule from the body of the block,
you might squeeze right through and rip the polypropylene and then will
need a razor blade. The other risk of incomplete separation is damaging
your specimen at the tip of the block. If complete separation is
accomplished, however, this rarely, if ever, happens.

One other advantage of this method is that, if you're gentle, you can
reuse the beem capsules and you don't ruin razor blades -- a real money
saver! (joke de jeur).

Try it.

John A.

--
John G. Aghajanian, Ph.D. } tel: 508 842-8921 ext.147
Worcester Foundation for Biomedical Research {fax: 508 842-9632
222 Maple Ave. } email: johna-at-sci.wfbr.edu
Shrewsbury, MA 01545




From: Susan Danielson :      sdaniels-at-post.its.mcw.edu
Date: Mon, 04 Nov 1996 12:45:07 -0800
Subject: microwave protocol TEM

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Message-ID: {327E55D3.AD8-at-post.its.mcw.edu}

Our laboratory is looking into purchasing a microwave for the processing
of muscle and nerve samples for TEM. Currently we are investigating
model 3400 from Ted Pella. Does anyone have any recommendations on this
product? We would first like to attempt a "trial run" using a
colleagues' microwave on our tissue samples just to see if this technique
would benefit our needs. We presently embed our samples in Spurr resin;
however do not have a microwave protocol. If anyone has a microwave
protocol that would work for our needs, we would appreciate a reply
greatly on this matter. Another question we are wondering about regards
venting of the microwave during processing for TEM. Is it necessary to
purchase a unit equipped witb a venting apparatus? Is placing the oven
in the hood a common practice? Any input on these questions would be
helpful to us. Thanks in advance,

Susan Danielson, MS
Neuromuscular Laboratory Coordinator
Medical College of Wisconsin


Thanks in advance




From: Neal Nicklaus :      nnicklaus-at-nova.sarnoff.com
Date: Mon, 04 Nov 1996 14:07:49 -0500
Subject: Re: Autofocus for light microscopy

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To John and the group in general:

I did not realize that autofocus was desired by many microscopists. Please
post the discussion to the group. I am also interested in autofocus for use
in my optical trap/fluorescence microscope. I need to move laterally a lot
in my thin film sample, eventually near 1 micron thickness, and do not want
to keep refocusing by hand. I do not know of any vendors in this area.


----------------
At 05:26 PM 11/1/96 -0500, you wrote:
} I keep hearing that autofocus is a desired feature on light microscopes. Is
} this really true or am I just an old fashioned knob turner.
}
} I would like to learn about your hands-on experiance with autofocus. Who are
} the major providers of autofocus? Who is the best supplier? What are the
} major applications for autofocus?
}
} Thanks for your help.
}
} John A. Reffner
}
} 102223.406-at-compuserve.com
}
}
}
}

Neal Nicklaus
Senior Scientist
SEQ Limited

Voice: 609-452-6033 Ext. 13
Fax: 609-452-5955
email nnicklaus-at-seq.sarnoff.com





From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Tue, 5 Nov 1996 11:20:54 GMT+1200
Subject: Re: Fried filament

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I had the same problem ie drastically-shortened filament life on my
JEOL JXA-5A. The chamber vacuum gauge showed the vacuum there to be
ok, but in the end the problem went away when I fixed one of the
solenoid valves which controls the compressed air that controls the
vacuum valves, so I concluded that it had been caused by a leak
around the gun, too small and too remote from the vacuum gauge
sensor to show up on the gauge. How sensitive/reliable is the
methanol-squirting test? Maybe not sufficient.
Good luck.

Ritchie Sims


} I have a Zeiss 940 SEM. A tungsten filament that can last for 200hr. is
} now fried in about 10 hr. A chunk o filament tip disappeared; the broken
} ends were thinned out and pointed like needle. A lot of black deposits
} were found inside and outside of the whenelt aperture disc and on the
} anode.
}
} Normally, I leave the saturation point untouched at the end of operation.
} On the next session, when Vacuum Ready light is lit, I push HT and
} Filament buttons, reset saturation point and off I go. It is equipped with
}
} a turbo pump, therefore, HT and filament must be turned off before
} specimen change and turned on again when the vacuum is ready. The
} filament would be turned on and off several times a day.
}
} I suspected a leak at the gun chamber but the vacuum remains stable
} when I squirted methanol at the gap.
}
} What is the cause of this problem? Any idea?

Ritchie Sims phone: 64 9 3737599 ext 7713
Department of Geology fax: 64 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: Mary Mager :      mager-at-unixg.ubc.ca
Date: Mon, 4 Nov 1996 20:22:05 -0800 (PST)
Subject: Re: Immersion Lens SEM

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Message-Id: {m0vKYzY-000UKCC-at-biolo.bg.fcen.uba.ar}

Dear Daved,
At 10:40 AM 11/2/96 -0500, you wrote:
} I am writing a research paper on Immersion Lens SEM for a class, and as
} part of my thesis, and I have found very little articles or Journals by
} searching, does anyone know of where I can find more info? Thanks
} --
} David Collins
I would suggest a call to a Hitachi representative for brochures and
articles about the S-570, S-800, S-900, S-4500 and other SEM's using the
immersion lens and upper detector. They may be able to refer you to more
specific articles.
Regards,
Mary
Mary Mager
Electron Microscopist
Metals and Materials Eng., UBC
6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel:604-822-5648, fax:604-822-3619
e-mail: mager-at-unixg.ubc.ca





From: Larnould :      larnould-at-mnet.fr
Date: Tue, 05 Nov 1996 11:20:14 +0100
Subject: Re: Fried filament

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At 11:20 05/11/1996 GMT+1200, you wrote:
} I had the same problem ie drastically-shortened filament life on my
} JEOL JXA-5A. The chamber vacuum gauge showed the vacuum there to be
} ok, but in the end the problem went away when I fixed one of the
} solenoid valves which controls the compressed air that controls the
} vacuum valves, so I concluded that it had been caused by a leak
} around the gun, too small and too remote from the vacuum gauge
} sensor to show up on the gauge. How sensitive/reliable is the
} methanol-squirting test? Maybe not sufficient.
} Good luck.
}
} Ritchie Sims
}
}
} } I have a Zeiss 940 SEM. A tungsten filament that can last for 200hr. is
} } now fried in about 10 hr. A chunk o filament tip disappeared; the broken
} } ends were thinned out and pointed like needle. A lot of black deposits
} } were found inside and outside of the whenelt aperture disc and on the
} } anode.
} }
} } Normally, I leave the saturation point untouched at the end of operation.
} } On the next session, when Vacuum Ready light is lit, I push HT and
} } Filament buttons, reset saturation point and off I go. It is equipped with
} }
} } a turbo pump, therefore, HT and filament must be turned off before
} } specimen change and turned on again when the vacuum is ready. The
} } filament would be turned on and off several times a day.
} }
} } I suspected a leak at the gun chamber but the vacuum remains stable
} } when I squirted methanol at the gap.
} }
} } What is the cause of this problem? Any idea?

Generally there is two main causes that destroy W filament:
-An overheating of the filament due to oversaturation or electronic
problem (gate transistor broken or leaking)
in that case life time is very short and both side of broken filement can
seen a "sphere" of melting tungnsten.
- In general case, the filament itself is etched by the ions of
remaining air around the filament and Wehnelt, so it's thinner and thinner
and finally break ( this explain why the saturation point is moving because
the resistance of filament is changing)
- In case of a bad vacuum or dirty vacuum due to backstreaming ,
outgasing... the etching power increase and life time fall down. Generally
this leak can't be seen on a chamber gauge (too far of gun) and it's too
small to check by ethanol and as in many case ready is given by a pirani
gauge or a timing after secondary pumping, you can't be sure of vacuum.
A good practice is to install a penning gauge close to the gun and check it
after all filament exchange or leaking of the column if no valve between gun
and column.
} } but in the end the problem went away when I fixed one of the
} } solenoid valves which controls the compressed air that controls
the valve.

Sometime a leak can come not from the outside of the valve but from the
compressed air that control that valve, (it's leaking by the axe) in that
case it's not possible to use ethanol except by introducing directly in
compressed air!

Theses explanations are not very scientific, they are an experience of the
field.
I apologize for my non academic English.
Salutations.


==========================================================
Jacky Larnould
tel 33 (0)4 67 72 28 26
fax 33 (0)4 67 79 54 90
email larnould-at-mnet.fr





From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 5 Nov 1996 11:57:42 +0100
Subject: 200CX holders

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Message-Id: {n1364927087.97411-at-ematserv.ruca.ua.ac.be}

REGARDING 200CX holders

Hi all,

we're looking for some replacements for our 200CX top entry 2.3 mm holders.
Anyone out there who has a spare one ?

Thanks,

Nick Schryvers
University of Antwerp, Belgium
schryver-at-ruca.ua.ac.be





From: Dr. Giovanni Iazzetti :      nanni-at-biol.dgbm.unina.it
Date: Tue, 05 Nov 1996 14:24:53 -0800
Subject: High Pressure Fixation for TEM

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Dear Fellows,
I need technical informations about high pressure fixation method
for TEM.
Thank you in advance.

G. Iazzetti
Dipartimento di Genetica, Biologia Generale e Molecolare
Universita' "Federico II" di Napoli
Italy
Iazzetti-at-biol.dgbm.unina.it




From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Tue, 5 Nov 1996 08:45:37 +0000
Subject: Re: afm tip characterization

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} I have produced some AFM tips (ESP single cantilever silicon) with small,
} non conductive crystals grown on the tip apex (crystal size is approx.
} 0.1- 0.5um) and I would like to characterize their morphology with either
} SEM or AFM.

snips ...

} If anyone has been successful in a similar endeavor, your suggestions
} would be most appreciated!
}
} Also, I have read a paper whose authors imaged an AFM tip using AFM but I
} have had little success with this technique. Has anyone succeeded in
} doing this who could offer suggestions?
}
} Sincerely,
}
} *****************************************************************
} Paul Demkowicz
} University of Florida

I haven't tried it but one instrument that would probably do what you want
is the Philips/Electroscan FEG ESEM which is certainly capable of producing
very good high magnification images from non-conducting specimens. I'd
guess most low vacuum/variable pressure FEG SEMs would produce some good
images.

Regards,

Larry Stoter






From: ebs-at-ebsciences.com
Date: Tue, 5 Nov 1996 08:41:01 -0600
Subject: Re: W filament failure

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Hi John & Colleagues-

A tungsten filament should *not* always burn out some distance from the tip.
However, it may be, if the filaments were not vacuum annealed, that the
"cold stress" imparted by the bending of the filament does indeed make a
"region of weakness."

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Tue, 5 Nov 1996 09:50:50 -0600
Subject: For John Chandler

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Message-Id: {199611051548.JAA29493-at-ux1.cso.uiuc.edu}
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John,
Please send me your email address--I need to ask a question or two
regarding federal accounting regulation references.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel *all mail:*
Microscopy
University of Illinois Station A
Rm 74 Bevier Hall PO Box 5037
905 S. Goodwin Ave. Champaign, IL 61825-5037 USA
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu
(217) 355-1143 home

*********** looking for a job again ***********






From: Chao-ying Ni; Office 312 SPL; Phone 1013 :      ni-at-me.udel.edu
Date: Tue, 5 Nov 1996 09:50:48 -0500 (EST)
Subject: X-sectional TEM specimen preparation of thin films

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Dear colleagues:

I need some technical information on the x-sectional TEM specimen
preparation of thin solid film multilayers. The layers are
glass(substrate)/Mo/CuInSe2/CdS/ZnO and the total thickness of the
film layers are ~5um. The tricky thing here is that the glass is very
brittle, and the adhesion between glass and Mo is not very good and
peeling may occur.

Any information on the x-sectional thin film specimen preparation in
general or specific techniques to deal with glass substrate would be
highly appreciated.

Chao-Ying Ni
Materials Science
University of Delaware
Newark, DE 19716













From: Jill Craig :      jcraig-at-unbc.edu
Date: Tue, 5 Nov 1996 08:04:47 -0800 (PST)
Subject: wanted: donations of old equipment working or not

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Hi,

I'm working on creating a museum exhibit on microscopes and microscopy
focusing on light microscopy and SEM.

If anyone has any old equipment for donation I would love to hear from
you. I'm still working on funding so I don't know yet how much I'll have
for shipping.

Also, any and all "cool" ideas for displays are always appreciated. I
need to fill two rooms with displays focussed strongly on the under 15 crowd.

The display is scheduled for next September so keep it in mind.

Thank you!

Jill




From: Klaus-Ruediger Peters :      Peters-at-BSAC.UCHC.EDU
Date: Tue, 5 Nov 1996 13:42:08 -0500
Subject: Resource for EMDE 2x2 Aluminum Slide Binders?

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Hi all Microscopists:

I am looking for a distributer of "Emde 2x2 Slide binders". I got them
always from EMDE Products, Torrance, CA. The aluminum slide binders for 2x2
slides (with thin anti-Newton ring glass) are very thin, easy to assemble
and have no degasing plastic part or moisture adsorbing materials. They
allow the mounting of 2x2 super slides, i.e., use of the entire square
projection area. EMDE is a product from Switzerland, sold in the US.

Thanks for your help and efforts. Klaus






From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Tue, 5 Nov 1996 07:36:17 -0500
Subject: FW: Fried filament

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} ----------
} From: Crossman, Harold
} Sent: Monday, November 04, 1996 11:00AM
} To: 'Ann FookYang (Ann-Fook Yang)'
} Subject: RE: Fried filament
}
} You may have a tight chamber but a leak elsewhere. Can you monitor the
} pressure in the gun when opening the column isolation valve? Does it
} increase more than say, 1/2 decade upon opening? If so, you may have
} excessive pressure in the sample chamber or a leaking seal around the
} isolation valve. How is the sample pressure monitored? Is there an
} ionization tube or some other appropriate gauge (NOT a Pirani or
} thermocouple tube!) on the chamber? Or, does the vacuum ready signal
} come on after a period of time with no gauging? If so, try to put a
} gauge on the chamber to read the actual pressure.
}
} Can you analyze the "black deposit" on the failed filament? If so,
} that may point you in the right direction.
}
} How was the gun housing cleaned when the previous filament died? Dirty
} solvent? Lint in chamber? Water? Finger prints?
}
} -------------------------------------------------
} Harold J. Crossman
} OSRAM SYLVANIA INC.
} Lighting Research Center
} 71 Cherry Hill Dr.
} Beverly, MA 01915
} Phone: (508) 750-1717
} E-mail: crossman-at-rd.sylvania.com
}
} Our web sites: www.sylvania.com
} www.osram.de
} www.siemens.com
} --
}
} "Crossman, Harold" {crossman-at-RD.SYLVANIA.com}
}
}




From: slakmon-at-scottscientific.com (P. Slakmon) (by way of Nestor J.
Date:
Subject: MICROWAVE/TISSUE PROCESSING WORKSHOPS

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Colleagues

Scott Scientific has submitted this posting to me for
review before posting to the Microscopy LIstserver.
I have judged it to be within the rules of the Listserver.

Nestor
Your Friendly Neighborhood SysOp
-----------------------------------------------

MICROWAVE/TISSUE PROCESSING SCIENTIFIC WORKSHOPS


Scott Scientific is in the process of organizing scientific workshops
on Microwave Processing which will be held in the different provinces of
Canada. We
would like to hear back from anyone who would be interested in attending
such workshops or volunteer their facilities (room or lab and perhaps an EM
to view final results). We also welcome inquiries from people outside of
Canada.

A nominal fee for the workshops will be charged to cover the travel,
lodging, etc... expenses of the invited speakers and assistants. The
registration fee will be based on the number of applicants, and
will only be used to cover the costs of the workshop. The larger the number
of participants at a location the lower the fee to attendees.

Firm dates have not yet been set and depend on the interest and attendance
for each location.

Our Scientific workshops will consist of rapid processing of tissue in a
research/clinical environment for electron microscopy.


FRESH TISSUE TO SECTION in three hours, using microwave processing.
Other microwave applications will be covered. Rapid immunostaining for LM or
EM, rapid paraffin processing and rapid decalcification.

We encourage people to bring there own samples, either fresh or in fixative.


Please direct all email inquiries to microwave-at-scottscientific.com

Scott Scientific
Ref: Microwave/Tissue Processing Scientific Workshop
PO Box 66552, Station Cavendish, Montreal, Quebec, H4W 3J6, Canada
Tel: 514-485-2309 Voicemail: 514-888-6509 Fax: 514-485-9931



Please fill out and return the following by email, fax or mail.
____________________________________________________
To: Scott Scientific
Ref: Scientific Workshop


Title:
FName:
LName:

Organization:
Dept:

Street Address:
Office Room Number:
Laboratory Room Number:
P.O. Box Address:
City:
Province/State:
Postal Code/Zip Code:
Country:

Telephone:
Ext:
Voicemail:
Pager:
Cellular:
Fax:
Email:
URL:

Are You Interested in Attending a Microwave Workshop:
Application Type or Field of Interest:

Have You Used a Microwave Oven:
How Long:

Comments:

Dates Available or of Interest for a Workshop:
How far would you be willing to travel to attend one of these workshops:

We would be willing to volunteer our lab or conference room for the use of a
workshop:


______________________________________________________
_______________________________________________
SCOTT SCIENTIFIC
P.O. Box 66552 Station Cavendish
Montreal, Quebec, H4W 3J6, Canada

Telephone: 514-485-2309 Fax: 514-485-9931
Voice Mail: 514-888-6509

WWW Site: http://www.scottscientific.com

E-Mail: info-at-scottscientific.com
sales-at-scottscientific.com
admin-at-scottscientific.com
links-at-scottscientific.com
_______________________________________________






From: ebs-at-ebsciences.com
Date: Tue, 5 Nov 1996 07:08:32 -0600
Subject: Re: microwave protocol TEM

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Dear colleagues,

Susan Danielson asked some questions regarding microwave protocols for TEM.
We have put a bibliography of papers regarding microwave polymerization of
TEM resins on-line at our WWW site (http://www.ebsciences.com). If you
don't have access to the Web, I can fax or mail it to you.

In our H2800 Microwave Processor, you can polymerize blocks of Spurr (or
other epoxy and acrylic) resin in open silicone rubber molds. It takes
about 30 minutes. The protocol is described in several papers by Beverly
Giammara.

We recommend *always* using a vented laboratory microwave for these
procedures. Our microwaves are vented at a rate of 100cfm. The venting
should also be "safety-interlocked", so that the microwave cannot be run if
the venting mechanism is blocked or not working properly. The venting
system both protects the user from breathing fumes when opening the cavity
door, and allows for the use of flammable reagents. It should not be
necessary to put the whole instrument in a fume hood; the instruments are
designed to accept ordinary clothes dryer hose, which can be run into the
hood. If a hood is not available, there are self-contained filtration
devices into which the fumes may be vented.

Disclaimer: Energy Beam Sciences manufactures a Microwave Processor for
electron and light microscopy.

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: Gary Login :      glogin-at-bih.harvard.edu
Date: Tue, 5 Nov 1996 10:23:27 -0400
Subject: Re: microwave protocol TEM

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Message-Id: {v03007802aea4f0010cbc-at-[134.174.111.7]}
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Susan, in response to your question regarding microwave processing, I
include the following information:


} Our laboratory is looking into purchasing a microwave for the
processing

} of muscle and nerve samples for TEM.

Some references of interest

1. Armati PJ, Pollard JD, Van Reyk D, Van der Lubbe L.
Neuroimmunological electron microscopy with microwave-accelerated
fixation. J Immunol Methods 1988;110: 267-9.

2. Tovar CA, Armati PJ, Pollard JD. Microwave fixation of whole
peripheral nerve for rapid and efficient enzyme-linked immunosorbent
assay (ELISA) screening of monoclonal antibodies. J Immunol Methods
1987;94: 127-30.

3. Bonner J, Armati P. microwave assisted staining of nerve and muscle
biopsy tissue. Biotechnic Histochem 1991;66: 236-8.

4. Eggli PS, Lucocq J, Ott P, Graber W, van der Zypen E.
Ultrastructural localization of hyaluronan in myelin sheaths of the rat
central and rat and human peripheral nervous systems using
hyaluronan-binding protein-gold and link protein-gold. Neuroscience
1992;48: 737-44.

5. Ashton FT, Bhopale VM, Fine AE, Schad GA. Sensory neuroanatomy of a
skin-penetrating nematode parasite: Strongyloides stercoralis. I.
Amphidial neurons. J Comp Neurol 1995;357: 281-95.

6. Feirabend HK, Kok P, Choufoer H, Ploeger S. Preservation of
myelinated fibers for electron microscopy: a qualitative comparison of
aldehyde fixation, microwave stabilisation and other procedures all
completed by osmication. J Neurosci Methods 1994;55: 137-53.

7. Bertrand N, Beley P, Beley A. Brain fixation for acetylcholine
measurements. J Neurosci Methods 1994;53: 81-5.

8. Login GR, Dvorak AM. Application of microwave fixation techniques in
pathology to neuroscience studies: A review. J Neurosci Methods
1994;55: 173-82.

9. Jensen FE, Harris KM. Preservation of neuronal ultrastructure in
hippocampal slices using rapid microwave-enhanced fixation. J Neurosci
Methods 1989;29: 217-30.

10. Login GR, Dvorak AM. Methods of microwave fixation for microscopy.
A review of research and clinical applications: 1970-1992. Prog
Histochem Cytochem 1994;27/4: 1-127.




} We would first like to attempt a "trial run" using a

} colleagues' microwave on our tissue samples just to see if this
technique

} would benefit our needs.


References useful for setting up a "trial run" in a new microwave oven
and for testing microwave-accelerated protocols:

1. Login GR, Tanda N, Dvorak AM. Calibrating and standardizing
microwave ovens for microwave-accelerated specimen preparation. A
review. Cell Vision 1996;3: 172-9.

2. Login GR, Dvorak AM. The Microwave Toolbook. A Practical Guide for
Microscopists (Beth Israel Hospital, Boston, 1994).





} We presently embed our samples in Spurr resin;

} however do not have a microwave protocol. If anyone has a microwave

} protocol that would work for our needs, we would appreciate a reply

} greatly on this matter.

References:

1. Login GR, Dvorak AM. The Microwave Toolbook. A Practical Guide for
Microscopists (Beth Israel Hospital, Boston, 1994). Chapter 10-
Microwave Curing

2. McLay AL, Anderson JD, McMeekin W. Microwave polymerisation of epoxy
resin: rapid processing technique in ultrastructural pathology. J Clin
Pathol 1987;40: 350-2.

3. Giammara BL, Birch DJ, Harper DO. Microwave polymerization of
embedding resins for biological/biomedical electron microscopy. In:
Snyder WB, Sutton WH, Johnson DL, Iskander MF, eds. Microwave
Processing of Materials II: Pittsburgh: Materials Research Society,
1991: vol. 189:347-53.

4. Giammara B. Microwave embedment for light and electron microscopy
using Epoxy resins, LR White, and other polymers. Scanning 1993;15:
82-7.

5. Kok LP, Boon ME. Microwave Cookbook for Microscopists (Coulomb
Press, Leyden, 1992).

6. Demaree RS, Jr, Giberson RT, Smith RL. Routine microwave
polymerization of resins for transmission electron microscopy. Scanning
1995;17: V25-6.

7. Giammara B. EM embedding media- A comparative overview. Electron
Microsc EMSA Tech Forum 1985;43: 1-7.




} Another question we are wondering about regards

} venting of the microwave during processing for TEM. Is it necessary
to

} purchase a unit equipped witb a venting apparatus? Is placing the
oven

} in the hood a common practice?


I recommend using a microwave oven in a lab hood whenever possible.
The high volume exhaust on laboratory microwave ovens available from
many manufacturers are an added safety feature which further reduce the
likelihood of being exposed to fumes from heated reagents when you open
the oven door.


Please write to me if you have any questions.



Dr. Gary R. Login

Dept. Pathology

Beth Israel Deaconess Medical Center

330 Brookline Avenue

Boston, MA 02215


phone: 617-667-2034

fax: 617-667-8676


e-mail: glogin-at-bidmc.harvard.edu





From: John.Wheatley-at-asu.edu (John C. Wheatley)
Date: Tue, 05 Nov 1996 14:25:28 -0700
Subject: Philips 400T

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Available: Philips 400T--twin lens and some attachments. Contact John
Wheatley at 602-965-3831 or via e-mail.

John C. Wheatley
Lab Manager
Center for High Resolution Electron Microscopy
BOX 871704
Tempe, AZ 85287-1704
Phone: (602) 965-3831
FAX: (602) 965-9004
John.Wheatley-at-ASU.Edu






From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Tue, 5 Nov 1996 15:19:37 -0500
Subject: RE: W filament failure

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Message-Id: {c=US%a=_%p=SYLVANIA%l=RD_EXC1-961105201937Z-1759-at-da-exc1.sylvania.com}

}
}
} Something I have never gotten a satisfactory explanation of is:
}
} Why does a W filament always (?) burn out some distance from the tip.
} Is
} this inherent to the bending of the metal and a region of weakness? And
} why
} is it always on the same side, relative to the power input?
}
}
} Perhaps I can shed some light on the subject. I am an engineer in a
} materials characterization lab for OSRAM SYLVANIA. We make tungsten
} and light bulbs. I have looked at a filament or two in the SEM.
}
} Tungsten SEM filaments are generally "pure" tungsten rather than doped,
} or alloyed, as is normally done for light bulbs. The pure tungsten
} tends to form relatively smooth grain boundaries across the wire
} section, called a "bamboo structure". The grain boundaries have a
} higher resistance than the crystals themselves and they tend to slide
} past each other. These two factors lead to decreasing cross-sectional
} area (the W evaporates faster in the hot spots) which in turn causes
} the filament to get hotter, which in turn accelerates evaporation
} which.....
}
} Also, certain tungsten oxides have a higher vapor pressure than the
} metal. Thus, residual oxygen (water vapor is particularly troublesome)
} reacts with the W surface and is immediately evaporated yielding
} another pristine, reactive surface. More removal.
}
} As far as weakness, the tungsten is fully annealed as soon as it is
} turned on. Pure tungsten relaxes well below 2000C and filaments can
} operate at much higher temperatures.
}
} Hope this helps.
} -------------------------------------------------
} Harold J. Crossman
} OSRAM SYLVANIA INC.
} Lighting Research Center
} 71 Cherry Hill Dr.
} Beverly, MA 01915
} Phone: (508) 750-1717
} E-mail: crossman-at-rd.sylvania.com
}
} Our web sites: www.sylvania.com
} www.osram.de
} www.siemens.com
} --
}
} "Crossman, Harold" {crossman-at-RD.SYLVANIA.com}
}
}
}
}
}




From: lizard-at-okway.okstate.edu (Ginger Baker)
Date: Tue, 5 Nov 1996 16:43:45 -0800
Subject: Staining question

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Mime-Version: 1.0

Hello all,

I was asked by a fellow researcher if she could stain LR White
sections with these two stains: H & E stain and Trichrome stain. The
tissue embedded in the LR White is fish epithelial tissue. I have not
used these stains with LR White. Has anyone else?

Thank you,

Ginger Baker
EM Lab Manager
250 Veterinary Medicine
Dept. of Anatomy, Pathology, and Pharmacology
Oklahoma State University
Stillwater, OK 74078
(405) 744-6765
FAX: (405) 744-5275
Email: lizard-at-okway.okstate.edu




From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Tue, 5 Nov 1996 17:45:09 -0500
Subject: Microsopy Equipment for Exhibit

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Message-Id: {v03007801aea5736de087-at-[206.69.208.21]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Jill

If you are in Chicago sometime, check out the Microscopy
Exhibit at the Museum of Science and Industry. It has a
wide variety of things, including x-sections of real microscopes
and a number of displays. Many of which are geared for
the 15 and under crowd.

Nestor
Your Friendly Neighborhood SysOp.






From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Tue, 5 Nov 1996 08:00:24 -0500
Subject: RE: W filament failure

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Message-Id: {c=US%a=_%p=SYLVANIA%l=RD_EXC1-961105130024Z-1562-at-da-exc1.sylvania.com}
"'johnf-at-geology.wisc.edu'" {johnf-at-geology.wisc.edu}

}
}
} Something I have never gotten a satisfactory explanation of is:
}
} Why does a W filament always (?) burn out some distance from the tip.
} Is
} this inherent to the bending of the metal and a region of weakness? And
} why
} is it always on the same side, relative to the power input?


Perhaps I can shed some light on the subject. I am an engineer in a
materials characterization lab for OSRAM SYLVANIA. We make tungsten and
light bulbs. I have looked at a filament or two in the SEM.

Tungsten SEM filaments are generally "pure" tungsten rather than doped,
or alloyed, as is normally done for light bulbs. The pure tungsten
tends to form relatively smooth grain boundaries across the wire
section, called a "bamboo structure". The grain boundaries have a
higher resistance than the crystals themselves and they tend to slide
past each other. These two factors lead to decreasing cross-sectional
area (the W evaporates faster in the hot spots) which in turn causes the
filament to get hotter, which in turn accelerates evaporation which.....

Also, certain tungsten oxides have a higher vapor pressure than the
metal. Thus, residual oxygen (water vapor is particularly troublesome)
reacts with the W surface and is immediately evaporated yielding another
pristine, reactive surface. More removal.

As far as weakness, the tungsten is fully annealed as soon as it is
turned on. Pure tungsten relaxes well below 2000C and filaments can
operate at much higher temperatures.

Hope this helps.
-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.osram.de
www.siemens.com
--

"Crossman, Harold" {crossman-at-RD.SYLVANIA.com}



}




From: F. H. Schamber :      fhscham-at-sgi.net
Date: Tue, 05 Nov 1996 21:39:39 -0500
Subject: Re: W filament failure

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Message-ID: {327FFA6B.507B-at-sgi.net}

ebs-at-ebsciences.com wrote:
}
} Hi John & Colleagues-
}
} A tungsten filament should *not* always burn out some distance from the tip.
} However, it may be, if the filaments were not vacuum annealed, that the
} "cold stress" imparted by the bending of the filament does indeed make a
} "region of weakness."
}
} Best regards,
} Steven E. Slap, Vice-President
} ********************************

Steven

I'm puzzled by your response. Every tungsten filament I've ever seen
which has expired due to "natural causes" (i.e., which has failed
through gradual metal evaporation, rather than overcurrent, bad vacuum,
etc.) has opened just a bit to one side of center. I don't think I've
ever seen a filament which has gradually thinned and then opened just at
the center of the bend, despite the fact that this is where one would
expect the highest temperature for a filament of uniform thickness.
This seems to be true for filaments of all manufacture, including those
from your company.

My speculation as to a reason for this behavior is that the bending
process causes a very slight thickening at the apex of the bend, with a
similarly slight thinning in the adjacent regions. Near the end of its
life, the slight variations in thickness create increasing differences
in local ohmic heating and thus evaporation in a regenerative process.
This theory may be incorrect, but it is substantiated by a lot of time
spent observing operating filaments via an optical device. What I
observed is that the filament wire seems to thin rather uniformly until
late in the filament's life. In the last hours of lifetime, the
differences in thinning become pronounced and then proceed to failure in
an exponential fashion. The thinning regions were always just next to
the apex. I have also speculated that there may be actual migration of
metal to the tip during this end-game, thus hastening the thinning from
the side.

Fred Schamber




From: Mike Witcomb :      MIKEW-at-gecko.biol.wits.ac.za
Date: Wed, 6 Nov 1996 09:32:06 GMT+2
Subject: TEM preparation of powders?

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I have never worked with powders and thus would appreciate advice on
sample preparation for TEM study of microstructure and analysis.
Two sets of samples have been given to us:

1) Nanocrystalline WC-Co powder
2) Milled FeSi, size 0.1mm diameter upwards to 2mm, many appear to be
hollow, the hole being completely enclosed by material.

Facilities available: Gatan Ultrasonic Drill, Gatan Dimpler, Gatan Duo
Ion Mill 600 (1980) with minimum angle of incidence about 13 degrees.

Thanks in advance for any suggestions.


Michael J Witcomb PhD
Electron Microscope Unit
University of the Witwatersrand
Private Bag 3
WITS
2050
South Africa

Telephone: + 27 11 716 4000
+ 27 11 716 2419 (messages)
Fax: + 27 11 339 3407
E-mail: mikew-at-gecko.biol.wits.ac.za





From: I.MacLaren-at-BHAM.AC.UK (Ian MacLaren)
Date: Wed, 6 Nov 1996 12:38:48 +0000
Subject: Re: TEM preparation of powders?

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Message-Id: {m0vL2BX-0000heC-at-rodange.crpcu.lu}

} I have never worked with powders and thus would appreciate advice on
} sample preparation for TEM study of microstructure and analysis.
} Two sets of samples have been given to us:
}
} 1) Nanocrystalline WC-Co powder
} 2) Milled FeSi, size 0.1mm diameter upwards to 2mm, many appear to be
} hollow, the hole being completely enclosed by material.
}
} Facilities available: Gatan Ultrasonic Drill, Gatan Dimpler, Gatan Duo
} Ion Mill 600 (1980) with minimum angle of incidence about 13 degrees.

Mike,
If your WC-Co powders are suitably fine (say less than 200-300 nm) then you
may be able to prepare specimens without resort to ion milling. You will
need to prepare some thin carbon films mounted on copper grids (which is
not difficult, I can provide details of how). You then disperse the powder
in a suitable medium, we usually use methanol, and dip the grid in. The
powder particles should then be stuck to the carbon film and can easily be
observed in the TEM.

I am not so sure about the FeSi but I would imagine something like mixing
with epoxy, curing and then preparing by cutting slices, drilling out
discs, dimpling and ion thinning may work. Alternatively, some people have
prepared similar powders by electroplating with Ni and electropolishing.

Hope this helps


=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=
=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=
=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD
Ian MacLaren, Tel: (44) (0) 121 414 3447
IRC in Materials for FAX: (44) (0) 121 414 3441
High Performance Applications, email: I.MacLaren-at-bham.ac.uk
The University of Birmingham, http://sun1.bham.ac.uk/I.MacLaren/
Birmingham B15 2TT,
England.
=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=
=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=
=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD=DD






From: Wharton Sinkler :      sinkler-at-apollo.numis.nwu.edu
Date: Wed, 6 Nov 1996 08:28:42 +0200 (MEZ)
Subject: Re: TEM preparation of powders?

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On Wed, 6 Nov 1996, Mike Witcomb wrote:

} I have never worked with powders and thus would appreciate advice on
} sample preparation for TEM study of microstructure and analysis.
} Two sets of samples have been given to us:
}
} 1) Nanocrystalline WC-Co powder
} 2) Milled FeSi, size 0.1mm diameter upwards to 2mm, many appear to be
} hollow, the hole being completely enclosed by material.
}
} Facilities available: Gatan Ultrasonic Drill, Gatan Dimpler, Gatan Duo
} Ion Mill 600 (1980) with minimum angle of incidence about 13 degrees.
}
} Thanks in advance for any suggestions.
}

Dear Mike:

You can use the ion mill. However, first you will need to press the powders
into 3mm pellets. We have done this with a die and hardened steel rods, which we had
made for the purpose. The die had a 3mm bore, into which the rods could be
inserted. Placing a small spoonful (30 mg should be plenty) of the powder
on top of one rod (inserted into the die), the second rod was inserted on
top and a force was applied (we used a little more than 2 tons). This
consolidated the pellet sufficiently for subsequent grinding, dimpling and
ion milling.

Short of having a die made, you could try any kind of press, hopefully with a
small diameter (in order to reach the necessary pressure), and then reduce the
consolidated powder to a 3mm pellet.

Sometimes milled powders tend not to stick together well. In this case you can
mix the powder with a nice ductile, slow-milling metal like Mo, in about 1:1
proportion. This eliminates the problem of crumbling during grinding.

If you need any more details, let me know.


Wharton

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Wharton Sinkler PhD
Department of Materials Science and Engineering
Northwestern University
2225 Sheridan Rd.
Evanston, IL 60208-3108
tel: (847) 491-7809
fax: (847) 491-7820
email: sinkler-at-apollo.numis.nwu.edu


}
} Michael J Witcomb PhD
} Electron Microscope Unit
} University of the Witwatersrand
} Private Bag 3
} WITS
} 2050
} South Africa
}
} Telephone: + 27 11 716 4000
} + 27 11 716 2419 (messages)
} Fax: + 27 11 339 3407
} E-mail: mikew-at-gecko.biol.wits.ac.za
}










From: Jason Taylor Smith :      jtsmith2-at-eos.ncsu.edu
Date: Wed, 06 Nov 1996 10:49:41 -0500
Subject: Spin Polarization Analysis

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Sender: jtsmith2-at-eos.ncsu.edu
Message-ID: {3280B395.328D-at-eos.ncsu.edu}

Hello,
I have been looking for information concerning Spin Polarization
Microscopy in the SEM (SEMPA). SEMPA is concerned with the ability to
determine the orientation of magnetic domains in a specimen by analysis
of the spin of the secondary electrons. Any information concerning
recent developments (publications?) in theory, or any organization that
has a product (spin polarization analyzer?) on the market that is
capable of this type of analysis would be greatly apreciated.
Thanks
Jason Smith
email: jtsmith2-at-eos.ncsu.edu




From: MaceyInc-at-aol.com
Date: Wed, 6 Nov 1996 12:00:22 -0500
Subject: Microscopy

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Please unsubscribe.

Thank you




From: S.Suder-at-eee.salford.ac.uk
Date: 6 Nov 96 17:48
Subject: Thin film TEM sample preparation by SACT

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Hi,Mr Ni,

I am preparing thin solid film TEM samples by the Small-Angle Cleavage
Technique(originated and developed by John McCaffrey) currently. But,
the substrates are Si and the total thickness of the films is
thinner than 500 nm.

If you use Si substrates instead of glass and limit your films
within 500 nm range in thickness, I suggest you may try this
method.

Good luck.

S Suder
Joule Physics Laboratory
University of Salford
Manchester M5 4WT
UK
Tel: +44 (0161) 745 5000x3264
Fax: +44 (0161) 745 5119
Email: s.suder-at-eee.salford.ac.uk





From: JMCLEAN-at-ISDTCP3.HWC.CA (J McLean)
Date: Wed, 6 Nov 1996 12:57:41 -0500
Subject: RE: autofocu capabilities

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Mime-Version: 1.0


I saw some inquiries regarding autofocus. We have 4 light microscopes
with autofocus capabilities. We could not aford 4 complete motorized
stages with autofocus so we found a company that modified our existing
stages and attached autofocus controls as well. I am not sure what
their current prices are like but you can contact them at
richard-at-brancker.ca for updated info.






From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Wed, 6 Nov 1996 10:41:02 -0500
Subject: Sputter coaters

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Message-Id: {c=US%a=_%p=SYLVANIA%l=RD_EXC1-961106154102Z-1919-at-da-exc1.sylvania.com}

Microscopists,

I am thinking about replacing my cantankerous sputter coater. I
currently use Au/Pd and am satisfied with the results when I can get
them. High resolution is not as important as complete coverage of
insulating materials. I am interested in reading user experiences, good
and bad, about features, product support, RELIABILITY, accuracy,
repeatability, etc. I am primarily interested in bench-top systems.
High through-put is not an issue. I use accelerating voltages from 500
to 30keV, various beam currents, etc. If the machine can do it, I use
it.

As this request is not an objective survey of instrument features, I
will keep all comments confidential. Please respond directly to me.

-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.osram.de
www.siemens.com
--

"Crossman, Harold" {crossman-at-RD.SYLVANIA.com}





From: McCaffrey, John :      John.McCaffrey-at-nrc.ca
Date: Wed, 6 Nov 1996 15:25:00 -0500
Subject: X-sectional TEM specimen preparation of

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Message-ID: {c=CA%a=_%p=NRC%l=NRC/IMSD/00046164-at-imsd-exchange.nrc.ca}
worldwide BB for microscopist
{microscopy-at-Sparc5.Microscopy.Com} ,
"McCaffrey, John"
{John.McCaffrey-at-nrc.ca}


Chao-Ying Ni wrote:

} I need some technical information on the x-sectional TEM specimen
} preparation of thin solid film multilayers. The layers are
} glass(substrate)/Mo/CuInSe2/CdS/ZnO and the total thickness of the
} film layers are ~5um. The tricky thing here is that the glass is very
} brittle, and the adhesion between glass and Mo is not very good and
} peeling may occur.

} Any information on the x-sectional thin film specimen preparation in
} general or specific techniques to deal with glass substrate would be
} highly appreciated.


The simplest techniqe, and the first that I would try, would be to
try the small-angle cleavage technique, but fracture the sample instead
of
cleaving it.
1. back-thin the sample to about 100 microns thickness
2. using a scriber on the BACK surface (the side that you
back-thinned),
make a series of scribe lines about 1- 2mm apart
3. fracture the sample along these lines (over the edge of a glass
coverslip
works well)
4. Using the scriber on the FRONT surface, make small scribe lines at a
10-
20 degree angle to the fractured edge, BUT NOT TO THE EDGE ITSELF! Keep
scribing along these small lines until the sample fractures into a
thin triangular-shaped needle. Make about eight of ten of these.
These
needles will have the original fractured edge intersecting the new
scribed/fractured edge at a fine point on the surface with the layers
on it. Pick out the "pointiest" of these - these are your samples!
5. Mount each sample on edge on a standard copper slot grid with a
viscous
epoxy (silver-filled epoxy works well).

For examples of the technique applied to layers on glass and other
non-
cubic crystal substrates, see Scott Walck's presentations at the spring
MRS
meeting in San Francisco, March 31-April 4/96 or at the spring Int'l.
Conference on Metallurgical Coatings and Thin Films in San Diego, April
21-25,
or contact him to ask for reprints (walcksd-at-ml.wpafb.af.mil).

For references on the technique applied to semiconductors, see J.P.
McCaffrey, "Improved TEM samples of semiconductors prepared by a
small-angle
cleavage technique", Microscopy Research and Technique 24:1890-184
(1993), or
email me and I'll send a short video that applies to both crystals and
amorphous materials.

Cheers
John
-----------------------------------------------------------------------
-
| John P. McCaffrey tel: Canada 613-993-7823 |
| National Research Council of Canada fax: Canada 613-990-0202 |
| Inst. for Microstructural Sciences _____ _____ |
| Montreal Road Labs, Bldg. M-50 | | __/\__ | | |
| Ottawa, Ontario, K1A 0R6 | | __/\\ //\__ | | |
| Canada | | \ \\ // / | | |
| | | /___ ___\ | | |
| email: john.mccaffrey-at-nrc.ca | | /__ __\ | | |
| | | || | | |
| |_____| |_____| |
-----------------------------------------------------------------------
-





From: William Tivol :      tivol-at-wadsworth.org
Date: Wed, 6 Nov 1996 17:14:11 -0500 (EST)
Subject: Re: Spin Polarization Analysis

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}
} Hello,
} I have been looking for information concerning Spin Polarization
} Microscopy in the SEM (SEMPA). SEMPA is concerned with the ability to
} determine the orientation of magnetic domains in a specimen by analysis
} of the spin of the secondary electrons. Any information concerning
} recent developments (publications?) in theory, or any organization that
} has a product (spin polarization analyzer?) on the market that is
} capable of this type of analysis would be greatly apreciated.

Dear Jason,
Another possibility is to generate a polarized electron beam,
e.g., with a Stern-Gerlach apparatus, and measure the right-left assym-
metry of the scattering. As this assymetry may be measurable with
commercially available detectors, producing a polarized beam may be the
easiest course.
Yours,
Bill Tivol




From: Albert Sicignano :      mks-at-cyburban.com
Date: Thu, 07 Nov 1996 00:06:52 -0500
Subject: Looking for old EDAX 9900 or 9800 EDS system

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I am trying to help our local high school setup and SEM and EDS system
for their earth science department. I located an old SEM and now need
an EDAX 9900 or 9800 EDS system for it. If anyone has one of these
systems I can arrange to pick it up. Thanks, Al Sicignano




From: Karen Klomparens :      kklompar-at-msu.edu
Date: Thu, 07 Nov 1996 11:14:49 -0400 (EDT)
Subject: De-waxing and epoxy emmbedding

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Message-Id: {199611070535.AAA17156-at-ns1.axs2000.net}
To: MICROSCOPY BB {Microscopy-at-Sparc5.Microscopy.Com}

I have been asked to forward the following inquiry to the group.

(Please do not reply to me reply to:
Karen Klomparens {kklompar-at-msu.edu}


------- Forwarded Message Follows -------

Popped the reprints in the mail to you today.

Would you put a question out on the list serve for me:

Does anyone have a foolproof (HA!) method to dewax thick paraffin sections
(of plants) on microscope slides and then to run them up in resin for TEM?
The key question, of course, is getting the sample off of the glass slide.
Any methods or references will be appreciated! Thank you.

Karen Klomparens
Center for Electron Optics
Michigan State University

Karen Klomparens {kklompar-at-msu.edu}





From: Crozier-at-asu.edu (Peter Crozier)
Date: Thu, 07 Nov 1996 09:56:45 -0700
Subject: De-waxing and epoxy emmbedding

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subscribe

Peter A. Crozier






From: Robert Mixon :      mixonr-at-ohsu.edu
Date: Thu, 07 Nov 1996 10:22:48 -0800
Subject: RETROVIRUS IDENTIFICATION

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Message-Id: {s281b888.017-at-ohsu.edu}
X-Mailer: Novell GroupWise 4.1

I WOULD LIKE SOME HELP IDENTIFYING SOME RETROVIRUS PARTICLES IN SOME OF MY
ELECTRON MICROGRAPHS. ANY IDEAS??????

BOB

MIXONR-at-OHSU.EDU




From: hawkey-at-neuro.duke.edu (larry hawkey)
Date: Thu, 7 Nov 1996 14:47:28 -0500
Subject: What is a Reichert Ultra cut E worth?

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Message-Id: {v01510100aea7ecab7de4-at-[152.3.72.63]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

We have Reichert Ultra cut E, Reichert knife breaker, (Really LKB with
Reichert logo) and a LKB auto stainer (for grids). We have shut down our
EM lab and my boss is thinking about selling them. Duke surplus has to
apraise them, but these guys don't really know what these things might be
worth. Does any one want to give me a guess as to what they might be
worth? All are about 6 years old and are working. When we sold the scope
there didnot seem to be much of a market, although, we did eventually sell
it. Is it amy better for microtomes and other lab stuff?

I thank you in advance for any helpful information.

larry hawkey
Former Electron Microscopist
Department of Neurobiology
Duke University

Larry Hawkey
hawkey-at-neuro.duke.edu
Department of Neurobiology
Duke University






From: Jake Schaper :      Jake_Schaper-at-chdqm.sps.mot.com
Date: 7 Nov 1996 13:31:26 -0700
Subject: Subject-EDS Donation

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Message-Id: {n1364748781.44387-at-chdqm.sps.mot.com}

I read the request for an old Spectrometer for a high school, but deleted
it. However, after deletion, I saw a used EDAX9100 for sale for $500 in
October's "Microscopy Today" magazine Page 20. Maybe it's worth a call by
whomever was searching for one. The listed phone number is 203-389-6065. No
names were listed. The area code looks like the eastern time zone.









From: Richard Thrift :      Richard_Thrift-at-depotech.com
Date: Thu, 07 Nov 1996 14:17:53 -0800
Subject: How to annotate tiff files?

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X-Mailer: Novell GroupWise 4.1

There must be a way, but I'm not aware of it. Can I add a
brief description of the sample to the tiff file, so that the
description will always stay associated with the image?

What software will allow me to read/write the description?
In general, what software is used to edit tiff files other than
editing the image itself?
I'm using a PC.

Thanks
Richard





From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Thu, 7 Nov 1996 16:31:30 -0700
Subject: TEM film price increase

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I just ordered some Kodak 4489 TEM film, and was shocked by the price
increase from my normal supplier. Our price went from about $45/100 sheets
to $70. Has anyone else had the same surprise?

I'd be interested in cheaper sources of this film or comparable EM films.
I have been told that the Ilford EM film has been discontinued.

John
chandler-at-lamar.ColoState.EDU






From: kna101-at-utdallas.edu
Date: Thu, 7 Nov 1996 15:52:28 -0600 (CST)
Subject: Re: De-waxing and epoxy emmbedding

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Karen,

I haven't dewaxed tissue before, but I have reembedded tissue on
a slide before. I stood a full beem capsule on top of the slide and
polymerized according to instuctions for the media I filled the capsule
with. To get the tissue off the slide, I placed the slide in a petri
dish full of propylene oxide for an hour or so, then I removed the stub,
tissue and all, with a razor blade.

I have heard it helps if the slide is coated with albumin prior
to placeing the waxed section on it.

Good luck,
Karen Pawlowski




From: Ann-Fook Yang (Ann-Fook Yang) :      YANGA-at-em.agr.ca
Date: Thu, 07 Nov 1996 13:36:57 -0500
Subject: summary, fried filament

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331 UUEncode TEST11.WPD

Many thanks to all who responded to my question, to Nestor and MSA
who provide this wonderful forum.

I have not yet found the source of my problem. I have removed an X-ray
detector with a new Be window installed. This may not be the problem.
The company which installed the window told me that they used TORR
SEAL and that vacuum was good (at least at the gauge). I also let the
machine pump for at least 10 min. before turning on HT and filament.
The filament is turned down every time and restarted slowly. There
were three filaments fried before I posted the question. So far, the
filament is behaving normally. It is going to take some time to sort out the
source of this problem.
I have summarized all the responses in the attached file.


Cheers,
Ann Fook Yang
{WP Attachment Enclosed}

begin 644 TEST11.WPD



From: Ann-Fook Yang (Ann-Fook Yang) :      YANGA-at-em.agr.ca
Date: Thu, 07 Nov 1996 13:36:57 -0500
Subject: summary, fried filament

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From: owl-at-owlsnest.com
Date: Thu, 7 Nov 1996 20:37:59 -0500 (EST)
Subject: Is Your Web Site A Secret?

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Is your web site the best kept secret on the Internet?

We'll post your site to 50 search engines/indexes for $85
and complete the job in two business days.

Please respond for details or call 914-278-4933.

-Julie

===Web Promotions=====Press Releases=====Link Exchanges=========
Owl's Eye Productions, Inc.
260 E. Main Street
Brewster, NY 10509
Ph: 914-278-4933 Fx: 914-278-4507 E-mail: owlseye-at-owlsnest.com




From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 07 Nov 1996 14:39:32 -0600
Subject: Re: De-waxing and epoxy emmbedding

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Message-Id: {199611072043.OAA20030-at-watson.bcm.tmc.edu}
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At 11:23 AM 11/7/96 -500, you wrote:

} Does anyone have a foolproof (HA!) method to dewax thick paraffin sections
} (of plants) on microscope slides and then to run them up in resin for TEM?
} The key question, of course, is getting the sample off of the glass slide.
} Any methods or references will be appreciated! Thank you.
***************************

Karen -

We do this somewhat routinely with H & E preps of human biopsies. First, we
soak off the coverslip with xylene. We then rehydrate the section on its
glass slide through a series of alcohols (100% =} water) and osmicate the
tissue, again on the slide. We then dehydrate the tissue back through 100%
alcohol and P.O. and flood the slide with PO/Spurr in 2:1, 1:1, 1:2, and
pure Spurr, all while the tissue is still on the glass slide. We then fill a
Beem capsule with resin and invert it over the section and oven cure it. The
cured (and stuck) capsule and slide is then dropped into a beaker of liquid
nitrogen until it is thoroughly chilled. The LN chilled slide/capsule is
then transferred quickly to a beaker of hot water. This frees the glass from
the block. Remember the tissue is right on the surface of the resin block
and the first section off the knife will have tissue in it. So align the
block in your microtome carefully.

I assume that you could collect your paraffin sections on glass slides,
deparaffinize them in the usual way, and proceed as above. If you do not
subject the tissue to H&E staining, etc., but osmicate and embed them right
after deparaffinizing, your EM results might be better than ours. Tissue
that has been embedded in paraffin, and then stained for light microscopy,
doesn't have a whole lot of ultrastructure left; although we can often get
our diagnosis. Plant tissue may stand up to this better than human tissue.

Good luck.
Joiner
************************


Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: Richard L. Lemaster :      lemaster_wmtrp-at-ncsu.edu
Date: Thu, 07 Nov 1996 21:34:41 -0500
Subject: residual stress measurement of tungsten carbide tools

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Message-ID: {32829C41.460A-at-ncsu.edu}

Would it be possible to measure residual stresses in tungsten carbide
cutting tools as a measure of the quality of the grinding (sharpening)
process? Carbide tools typically have between 3 - 15% cobalt as a
binder.
In grinding these tools, a visual inspection after grinding may show a
good cutting edge even though the integrity of the material has been
compromised by improper grinding techniques.

Thanks




From: owl-at-owlsnest.com
Date: 11/7/96 6:51 PM
Subject: Re: Is Your Web Site A Secre

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Message-ID: {n1364728589.55958-at-macmail.lbl.gov}

We'll post your site to 50 search engines/indexes for $85
and complete the job in two business days.

Please respond for details or call 914-278-4933.

-Julie

===Web Promotions=====Press Releases=====Link Exchanges=========
Owl's Eye Productions, Inc.
260 E. Main Street
Brewster, NY 10509
Ph: 914-278-4933 Fx: 914-278-4507 E-mail: owlseye-at-owlsnest.com






From: Gang REN :      ren-at-image.blem.ac.cn
Date: Fri, 8 Nov 1996 13:21:47 -0800
Subject: Re: How to annotate tiff files?

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"How to annotate tiff files?" (Nov 7, 2:17pm)
References: {s281efa2.092-at-depotech.com}
X-Mailer: Z-Mail (3.1.0 22feb94 MediaMail)
To: Richard Thrift {Richard_Thrift-at-depotech.com} ,
Microscopy-at-Sparc5.Microscopy.Com

On Nov 7, 2:17pm, Richard Thrift wrote:
} Subject: How to annotate tiff files?
} There must be a way, but I'm not aware of it. Can I add a
} brief description of the sample to the tiff file, so that the
} description will always stay associated with the image?
}
} What software will allow me to read/write the description?
} In general, what software is used to edit tiff files other than
} editing the image itself?
} I'm using a PC.
}
} Thanks
} Richard
} -- End of excerpt from Richard Thrift

Dear Richard,

I know two softwares in PC which can open and annotate in tiff files, one is
PhotoStyler, another is ImageStar.

regards,
G.REN



--
+---------------------------------------------------------+
| Gang REN, Ph.D Candidate |
| Email: ren-at-image.blem.ac.cn Fax: (+86-010) 62561422 |
| Phone: (+86-10) 62568304; 62612498 |
| Beijing Laboratory of Electron Microscopy |
| Chinese Academy of Sciences, P.O Box 2724 |
| Beijing 100080, P.R.China |
+---------------------------------------------------------+







From: Zhiyu Wang :      zhiyu-at-hawaii.edu
Date: Thu, 7 Nov 1996 22:20:39 -1000
Subject: Re: How to annotate tiff files?

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Microscopy-at-Sparc5.Microscopy.Com


Hi, Rich:

One of PC software called Mocha 2.1 can open TIFF files and annotate on
the image in form of overlay. The overlay can be merged in image or
saperated. The annotate can be saved as a overlay file. You can open the
overlay file to see the annotate. The software can provide 3
overlaies in different color.
Hopefull it helps.

Zhiyu Wang
Dept. of Biosystem Engineering
University of Hawaii


On Fri, 8 Nov 1996, Gang REN wrote:

} On Nov 7, 2:17pm, Richard Thrift wrote:
} } Subject: How to annotate tiff files?
} } There must be a way, but I'm not aware of it. Can I add a
} } brief description of the sample to the tiff file, so that the
} } description will always stay associated with the image?
} }
} } What software will allow me to read/write the description?
} } In general, what software is used to edit tiff files other than
} } editing the image itself?
} } I'm using a PC.
} }
} } Thanks
} } Richard
} } -- End of excerpt from Richard Thrift
}
} Dear Richard,
}
} I know two softwares in PC which can open and annotate in tiff files, one is
} PhotoStyler, another is ImageStar.
}
} regards,
} G.REN
}
}
}
} --
} +---------------------------------------------------------+
} | Gang REN, Ph.D Candidate |
} | Email: ren-at-image.blem.ac.cn Fax: (+86-010) 62561422 |
} | Phone: (+86-10) 62568304; 62612498 |
} | Beijing Laboratory of Electron Microscopy |
} | Chinese Academy of Sciences, P.O Box 2724 |
} | Beijing 100080, P.R.China |
} +---------------------------------------------------------+
}
}
}
}





From: rw9-at-psu.edu (Rosemary A. Walsh) (by way of Nestor J. Zaluzec)
Date: Fri, 8 Nov 1996 07:12:44 -0500
Subject: Sputter coater

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We are repairing the sputter head on a 3 yr. old
SCD 050 Sputter-coater (BALTEC). Has anyone
done the same? Vacuum is fine but the problem
seems to be in the high V. We've changed the Oring,
shaft seal and now are working on the optocouplers.
Thanks in advance for any time and thought you can
give to this.
Rosemary Walsh






From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Fri, 8 Nov 1996 08:07:28 -0500
Subject: RE: How to annotate tiff files?

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Message-Id: {c=US%a=_%p=SYLVANIA%l=RD_EXC1-961108130728Z-2558-at-da-exc1.sylvania.com}
"'microscopy'"
{Microscopy-at-Sparc5.Microscopy.Com}

Richard,
Do you really want to annotate the image itself or catalog it for future
reference? If the latter is true, check out Thumbs Plus image database.
It allows annotation, keyword searches, thumbnail creation, and all
sorts of other neat stuff. It translates a handful of image formats and
runs under all Windows GUI/OS. The icing on the cake? It's about
sixty-five dollars.

Cerious Software, Inc.
1515 Mockingbird Lane
Suite 209
Charlotte, NC 28209
(704) 529-0200

www.cerious.com

I have no financial or other interest in Cerious. I am just a very
happy customer.

-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.siemens.com
--

} "Crossman, Harold" {crossman-at-RD.SYLVANIA.com




From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Fri, 8 Nov 1996 09:00:29 EST
Subject: Re: RETROVIRUS IDENTIFICATION

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} I WOULD LIKE SOME HELP IDENTIFYING SOME RETROVIRUS PARTICLES IN SOME OF MY
} ELECTRON MICROGRAPHS. ANY IDEAS??????

Bob,

My lab has done a bit of retrovirus diagnostic work from time to
time, mostly avian but some mammalian. What exactly do you
want....confirmation or id down to type?



-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: rgrappe-at-MMM.COM
Date: Fri, 08 Nov 1996 10:05:17 -0600
Subject: Attaching Laser to Optical Microscope

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I have had a request to attach a laser to my Ziess Axioplan
Light Microscope. The requester would like to irradiate a material with
the laser through the microscope and then record the results or the
process on video. Can someone recommend articles that might describe
how to attach a laser or a researcher who has done this.

Thanks for your help

Rod Rappe




From: Dr. H. Adelmann :      106421.3362-at-CompuServe.COM
Date: Fri, 8 Nov 1996 02:17:56 -0500
Subject: Subject: How to annotate tiff files

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Hello everybody,

All major scientific image processing software packages are able to
annotate TIFF files.
I made best experience with Media Cybernetics' Image-Pro Plus !
(....www.mediacy.com)

Dr. Holger G. Adelmann
Leverkusen, Germany




From: ebs-at-ebsciences.com
Date: Fri, 8 Nov 1996 12:20:25 -0600
Subject: Re: TEM film price increase

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Dear John and colleagues-

Energy Beam Sciences has, for some years now, sold AGFA SCIENTIA 23D56 film
for TEM. The price is comparable to the Kodak, and it offers certain
technical advantages.

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: Joyce Craig :      J-Craig-at-csu.edu
Date: Fri, 8 Nov 1996 12:30:32 -0600 (CST)
Subject: subscribe

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X-Authentication-Warning: ecom3.ecn.bgu.edu: bafpjec owned process doing -bs

subscribe listserver





From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Fri, 8 Nov 1996 14:24:35 EST
Subject: TEM film

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Someone recently posted a message lamenting the current price of
4489. After discarding the posting, I received a catalog from Laube
Photo offering the film for: 51.49/100 or 107.91/250. Not too bad!

The address is:

Laube Photo and Camera Supply
151 West Exchange Street
Akron, OH 44301

(330)535-3379 Voice
(330)535-0342



-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Fri, 8 Nov 1996 08:59:50 -0500 (EST)
Subject: Re: TEM film price increase

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On Thu, 7 Nov 1996, John Chandler wrote:

} I just ordered some Kodak 4489 TEM film, and was shocked by the price
} increase from my normal supplier. Our price went from about $45/100 sheets
} to $70. Has anyone else had the same surprise?
}
} I'd be interested in cheaper sources of this film or comparable EM films.
} I have been told that the Ilford EM film has been discontinued.
}
} John
} chandler-at-lamar.ColoState.EDU
}

John,
Yep! Too much! You can get 4489 through Penn Camera for approx. $57/box.
I order the bulk pack, cat. # 166-2238, 250 sheets for $112/box. It's
like getting 50 sheets free. Penn Camera can be reached at:
penncamera-at-aol.com address it to Jeff.
voice: 1-800-347-5770
Jeff and Art at this company are very good to deal with. Penn is the best
photo dealer I've dealt with in the past 17 years. Jeff and Art can help
with just about any problem you may have concerning photography. Like I
said before, I've dealt with Penn Camera for 17 years and they have always
been a help.



Peace through Christ,

Phil Rutledge, Director e-mail: prutle1-at-gl.umbc.edu
Center for Microscopy voice: (410) 455-3582
UMBC Dept. of Biology fax: (410) 455-3875
Catonsville, MD 21228
/////
/ /
/ /
/////// ///////
/ /
/////// ///////
/ /
/ /
/ /
/ /
/ /
/ /
/////





From: Richard Beanland +44 1327 356363 :      richard.beanland-at-gecm.com
Date: Fri, 08 Nov 1996 15:37:01 +0000 (GMT)
Subject: TEM - image simulation

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Disclose-Recipients: prohibited

Dear All,
I would like to simulate thickness fringes in a cleaved edge
semiconductor specimen with the aim of measuring composition of AlGaAs, InGaP
etc.. If anyone has an idea where I might be able to get the appropriate
software, comments on the feasibility of the measurement, etc., I would be
very glad to hear from them.

Thanks very much in advance,

Richard Beanland
GMMT Ltd.,
Caswell,
Towcester,
Northants NN12 8EQ
UK
e-mail richard.beanland-at-gecm.com
Tel. +44 1327 356363





From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 8 Nov 1996 10:06:04 -0500
Subject: Web site pub

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I agree that there is no need to pay a fee to have sites announced. Try
"Submit it" {http://www.submit-it.com/} . The site is sent automatically to a
bunch of users and engines. Most engines out there come to you for inclusion
is the site is visited often enough!

________ ___________
/ ______/ / _________/ Cesar Danilo Fermin, Ph.D.
/ / / / Professor of Pathology & Otolaryngology
/ / / /_____
/ \______ / ______/ Fax 504 587-7389 & Voice 584-2521
/________/ / / Internet: fermin-at-tmc.tulane.edu
/_______ \/_/
| | | | http://www1.omi.tulane.edu/departments/
| | | | pathology/fermin/cdftop.html
| |______/ |
|________ /




From: Dr. Augusto A. Morrone :      amorr-at-mse.ufl.edu
Date: Fri, 8 Nov 1996 15:21:54 -0500
Subject: Reflection electron diffraction in the TEM

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Dear Microscopy Netters:

I would like to get information on reflection electron diffraction
in the TEM. Recently, someone asked me if we had on our JEOL 200CX an
attachment to do reflection electron diffraction. I heard this attachment
was more common in older JEOL TEMs (JEM 7 or 9), and that it is installed in
the column somewhere below the OL or even below the intermediate lens. Does
anybody have this facility, or has any information about it?

Augusto A. Morrone
107D-MEL, P.O. Box 116400
MAIC
Materials Science and Engineering
University of Florida
Gainesville, FL 32611
(352) 392-1497 or 6985
Fax: (352) 392-0390
amorr-at-mse.ufl.edu





From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Fri, 8 Nov 1996 16:17:36 +0000
Subject: Re: residual stress measurement of tungsten carbide tools

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} Would it be possible to measure residual stresses in tungsten carbide
} cutting tools as a measure of the quality of the grinding (sharpening)
} process? Carbide tools typically have between 3 - 15% cobalt as a
} binder.
} In grinding these tools, a visual inspection after grinding may show a
} good cutting edge even though the integrity of the material has been
} compromised by improper grinding techniques.
}
} Thanks

Depends at what scale you are interested in examining the tools.

For 'macro' analysis, I believe that there are X-ray diffraction methods,
working on the scale of mm (and up) which use the broadening of diffraction
lines to measure strain.

On a 'micro' scale (micrometres down to nm), it is possible to use TEM
convergent beam electron diffraction to measure strain. Essentially the
same idea - you look at the broadening of diffraction lines.

However, I guess the XRD approach is potentially non-destructive whereas
the TEM method requires preparation of suitable specimens - which might
lead either to the release of residual stress, or introduce further stress.
If you are careful and use only electro-spark errosion, you should avoid
introducing more strain but it might be difficult to avoid stress release.

Regards,
Larry Stoter






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Fri, 08 Nov 1996 09:22:53 -0600
Subject: Re: How to annotate tiff files?

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Message-Id: {199611081526.JAA15802-at-watson.bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Most image cataloging, and some image analysis, software allow you to do
this. The standard is Adobe Photoshop. A smaller, but very capable image
databasing package with basic image editing capabilities, is Ulead, Inc.'s
Image Pals. Actually Image Pals has been updated and improved, and renamed;
and I can't recall the new name. Any good software shop would have both of
these programs. They allow you to store images with user provided titles,
keywords, and annotations that can be searched. An important feature is
"thumbnails" which allow you to preview small snapshots of many of your
images on screen at one time. Other features of these programs that are
useful are format conversion, file compression, and of course the various
image enhancement (sharpening, contrast control, etc.) features which
require care when used in a scientific environment.


At 02:17 PM 11/7/96 -0800, you wrote:
} There must be a way, but I'm not aware of it. Can I add a
} brief description of the sample to the tiff file, so that the
} description will always stay associated with the image?
}
} What software will allow me to read/write the description?
} In general, what software is used to edit tiff files other than
} editing the image itself?
} I'm using a PC.
}
} Thanks
} Richard
}
}
}
____ _
| ___\_\_o____/_| Joiner Cartwright, Jr., Ph.D.
{- [____\_\_---------- {...............Baylor College of Medicine
| o' Houston, Texas U.S.A.







From: Mark Wall :      Mark.Wall-at-quickmail.llnl.gov
Date: 8 Nov 1996 16:21:22 -0800
Subject: Mark Wall

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Message-ID: {n1364652238.19209-at-quickmail.llnl.gov}

Looking for a Side Entry Goniometer (SEG) for a JEOL 200/100 A or B or CX etc.
Preferably something to be given away from an unwanted TEM. (We don't need the
TEM though!)

Thanks

Mark Wall
Lawrence Livermore National Lab
510 423-7162





From: Karen Klomparens :      kklompar-at-msu.edu
Date: Thu, 07 Nov 1996 11:14:49 -0400 (EDT)
Subject: De-waxing and epoxy emmbedding -Reply

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Message-Id: {s2832fcb.036-at-TVMDL.TAMU.EDU}
X-Mailer: Novell GroupWise 4.1
microscopy-at-sparc5.microscopy.com

Karen,
Here's what I do when I need to embed paraffin sections on slides. Use
a diamond pencil to mark back of slide with area of interest. After
removing coverslip with xylene, deparaffinize tissue by soaking in xylene
for a couple of hours. Remove excess tissue with razor blade.
Rehydrate with EtOH , then wash in NaCacodylate. Postfix with OsO4,
wash and enbloc stain U.A. Dehydrate with EtOH, then P.O. Infiltrate
with 1:1 Epon Araldite : P.O. Drain in warm oven on side. Put on 100%
Epon Araldite, drain, and repeat. Put 1-2 drops of resin on tissue. Use a
flat hardened block that you have previously made in a Beem capsule (
add resin to capsule, close lid , and bake standing up on lid). Use the flat
end of this block and place it on top of slide, being careful not to
introduce air bubbles. Bake. Scrape excess resin from around block,
then place on 90 degree C. hotplate. After a few minutes, pop capsule (
with tissue attached) off with pliers. This is not always foolproof, but it
usually works for the few samples I do. You can also immerse slide in
liq. nitrogen which will break from the capsule. In the past, I've tried
inverting a capsule filled with fresh resin over the tissue, but I always
ended up with a mess because the resin would leak out.. I've also tried
putting the slide on top of an overfilled been capsule, then baking, but the
slide moves and I end up with air bubbles. My samples are animal tissue,
so you may have to modify it for plants.
Good luck!

Debbie Cassout
Texas Veterinary Medical Diagnostic Laboratory
E.M. Lab
College Station, TX
e-mail: dcassout-at-tamu.edu

} } } "Richard E. Edelmann" {edelmare-at-CASMAIL.MUOHIO.EDU}
11/07/96 11:23am } } }
I have been asked to forward the following inquiry to the group.

(Please do not reply to me reply to:
Karen Klomparens {kklompar-at-msu.edu}


------- Forwarded Message Follows -------

Popped the reprints in the mail to you today.

Would you put a question out on the list serve for me:

Does anyone have a foolproof (HA!) method to dewax thick paraffin
sections
(of plants) on microscope slides and then to run them up in resin for
TEM?
The key question, of course, is getting the sample off of the glass slide.
Any methods or references will be appreciated! Thank you.

Karen Klomparens
Center for Electron Optics
Michigan State University

Karen Klomparens {kklompar-at-msu.edu}






From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Sat, 9 Nov 1996 09:43:48 +0000
Subject: Re: TEM - image simulation

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} Dear All,
} I would like to simulate thickness fringes in a cleaved edge
} semiconductor specimen with the aim of measuring composition of AlGaAs, InGaP
} etc.. If anyone has an idea where I might be able to get the appropriate
} software, comments on the feasibility of the measurement, etc., I would be
} very glad to hear from them.
}
} Thanks very much in advance,
}
} Richard Beanland
} GMMT Ltd.,
} Caswell,
} Towcester,
} Northants NN12 8EQ
} UK
} e-mail richard.beanland-at-gecm.com
} Tel. +44 1327 356363

I guess in principle that is possible although I would have doubts as to
its practical implementation. I would first suggest (if you haven't already
done so) going through the theory and determining how acurately you can
measure the various parameters, and how sensitive the whole process is to
composition.

I presume that you plan to evaluate composition via the extinction
distance? However, if using thickness fringes, this also requires a
knowledge of thickness and you will need to determine the thickness
profile.

If you really want to try this route, I would suggest convergent beam
diffraction. Under appropriate diffraction conditions, you can count
Kossel-Mollenstedt fringes and measure their spacing in the CBD disc. A
simple plot gives you the thickness (from the intercept) and the extinction
distance (from the slope). This approach has two main advantages:

1. You are doing the analysis at a point, so in principle this will allow
you to pick up any compositional inhomogeneities, which a thickness fringe
approach would average out.

2. You can, if necessary, do it with a calculator and piece of graph paper!
No computer simulations will be needed.

Of course, it has disadvantages:

1. Again it presupposes that the extinction distance is a sensitive measure
of composition, as does the use of thickness fringes.

2. It won't work with very thin crystals - you need at least 3 dark fringes
on either side of the central bright maximum to do the plot (and I'd
suggest you need more to improve the accuracy).

3. You will probably need a double tilt holder to set up an appropriate
diffraction condition, and even then depending on the composition and
orientation of the specimen, you may not be able to find a suitable
orientation.

I presume you have considered direct analysis, that is EDX or EELS, and
concluded that accuracy is insufficent for the compositional changes you
are trying to measure?

How about measurement of lattice parameter? This may be sensitive enough to
show the compositional changes in which you are interested. There are two
approaches here:

1. Direct high resolution TEM - depends on the specimen and access to a
good instrument.

2. Back to convergent beam - the position of HOLZ lines is very sensitive
to lattice parameter, something like +/- 5 x 10-4 nm relative to a known
standard (it is not very good for absolute measurements). It does depend on
the TEM having a very stable kV, but this shouldn't be a problem on a
modern TEM.

Only having a rough idea of what you want to do, I'd recommend the last method.

Hope that is useful,

Regards,

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
---------------------------------------------------------------






From: Steven S. Trail :      trail-at-cyberdrive.net
Date: Sat, 09 Nov 1996 10:12:07 -0800
Subject: m-probe, Kevex - program to convert sys75.kvx file?

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {2.2.32.19961109181207.006b9478-at-pop.cyberdrive.net}
X-Sender: trail-at-pop.cyberdrive.net
X-Mailer: Windows Eudora Pro Version 2.2 (32)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I need to move a sys75.kvx file off of a DEC lsi 1173 and onto a p.c.
Once on the p.c., I would like to analyze the eds spectra (print out a
spectra and extract semi-quantitative elemental composition - and I
would like for it to be user friendly (boy, am I asking for a lot, or what!)

I am praying that the DEC has a copy of kermit on it. If not, then I will
have to either copy files one by one over the printer port to a laptop with
kermit
on it or someone get a copy of kermit onto the DEC.

Some other information: DEC LSI 1173 running on RT11, Kevex 9000 system
7579 delta5.
The DEC is connected to the outside world by 10 MB disks...

Is there any hope?

thanks,

Steve

***************************************
Dr. Steven S. Trail
B.P. Chemicals
4440 Warrensville Center Rd
Warrensville Hts., OH 44128
(216) 586-5136 (work)
(216) 586-5030 (fax)
trailss-at-bp.com
trail-at-cyberdrive.net (home)
***************************************





From: Steven S. Trail :      trail-at-cyberdrive.net
Date: Sat, 09 Nov 1996 10:12:07 -0800
Subject: m-probe, Kevex - program to convert sys75.kvx file?

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {2.2.32.19961109181207.006b9478-at-pop.cyberdrive.net}
X-Sender: trail-at-pop.cyberdrive.net
X-Mailer: Windows Eudora Pro Version 2.2 (32)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

I need to move a sys75.kvx file off of a DEC lsi 1173 and onto a p.c.
Once on the p.c., I would like to analyze the eds spectra (print out a
spectra and extract semi-quantitative elemental composition - and I
would like for it to be user friendly (boy, am I asking for a lot, or what!)

I am praying that the DEC has a copy of kermit on it. If not, then I will
have to either copy files one by one over the printer port to a laptop with
kermit
on it or someone get a copy of kermit onto the DEC.

Some other information: DEC LSI 1173 running on RT11, Kevex 9000 system
7579 delta5.
The DEC is connected to the outside world by 10 MB disks...

Is there any hope?

thanks,

Steve

***************************************
Dr. Steven S. Trail
B.P. Chemicals
4440 Warrensville Center Rd
Warrensville Hts., OH 44128
(216) 586-5136 (work)
(216) 586-5030 (fax)
trailss-at-bp.com
trail-at-cyberdrive.net (home)
***************************************





From: Sara Miller :      saram-at-acpub.duke.edu
Date: Sat, 9 Nov 1996 17:03:27 -0500 (EST)
Subject: Re: RETROVIRUS IDENTIFICATION

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On Fri, 8 Nov 1996, Buddy Steffens wrote:

} Date: Fri, 8 Nov 1996 09:00:29 EST
} From: Buddy Steffens {STEFFENS.B-at-calc.vet.uga.edu}
} To: Robert Mixon {mixonr-at-ohsu.edu} , Microscopy-at-Sparc5.Microscopy.Com
} Subject: Re: RETROVIRUS IDENTIFICATION
}
} } I WOULD LIKE SOME HELP IDENTIFYING SOME RETROVIRUS PARTICLES IN SOME OF MY
} } ELECTRON MICROGRAPHS. ANY IDEAS??????
}
} Bob,
}
} My lab has done a bit of retrovirus diagnostic work from time to
} time, mostly avian but some mammalian. What exactly do you
} want....confirmation or id down to type?
}
}
}
} -=W.L. Steffens=-
} Department of Veterinary Pathology
} College of Veterinary Medicine
} University of Georgia

Hi Buddy,}

Ditto above. Give me a call, and I'll try to stear you.
Sara


Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: Hugh Smith :      mms3-at-ix.netcom.com
Date: Sun, 10 Nov 1996 09:04:36 -0800
Subject: Phase Contrast Microscopy and Live Blood Analysis

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Message-ID: {32860B24.1D63-at-ix.netcom.com}

Looking for information re: the use of phase contrast microscopy and live
bllod analysis. Especially interested in any research done (or being
done) in the field.
Thanks,
Hugh Smith




From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Sun, 10 Nov 1996 16:07:48 +0100 (MET)
Subject: Re: TEM film: AGFA

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Do you have such nice information about AGFA 23D56?

Thanks

On Fri, 8 Nov 1996, Buddy Steffens wrote:

} Someone recently posted a message lamenting the current price of
} 4489. After discarding the posting, I received a catalog from Laube
} Photo offering the film for: 51.49/100 or 107.91/250. Not too bad!
}
} The address is:
}
} Laube Photo and Camera Supply
} 151 West Exchange Street
} Akron, OH 44301
}
} (330)535-3379 Voice
} (330)535-0342
}
}
}
} -=W.L. Steffens=-
} Department of Veterinary Pathology
} College of Veterinary Medicine
} University of Georgia
}

Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Lluis Sole i Sabaris, 1-3
E-08028 BARCELONA ESPANYA

Tel +34 (9)3 402 16 95
Fax +34 (9)3 402 13 98





From: IAN HALLETT :      ihallett-at-MARCCRI.MARC.CRI.NZ
Date: Mon, 11 Nov 1996 11:00:01 GMT+1200
Subject: Microscopy 97 - New Zealand Microscopy Conference

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Microscopy 97
Auckland 10-14 February 1997
Organised by Microscopy New Zealand (Inc.)

Something for everyone using or interested in microscopy

Workshops, Technical Sessions, Papers,

GUEST SPEAKERS

Prof. Anthony S-Y Leong,The Chinese University of Hong Kong.
Immunohistochemistry and microwave techniques in pathology.

Dr Ron Milligan, The Scripps Research Institute, USA
High resolution and low temperature TEM, image processing, particular
in the study of molecular motors.

Dr Val Randle, University College Swansea, UK
Electron backscattered kikuchi pattern analysis

Dr E.D. Salmon, The University of North Carolina, USA
High resolution video-enhanced microscopy of cell motility and the
cytoskeleton.

For Further Details Contact

Dr Ian Hallett
Organising Committee - Microscopy =9197 HortResearch, Private Bag 92
169, Auckland, New Zealand

Telephone +64-9-849 3660
Facsimile +64-9-815 4201
EMail ihallett-at-hort.cri.nz,


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660
EMail ihallett-at-hort.cri.nz




From: Ritchie Sims :      r.sims-at-auckland.ac.nz
Date: Mon, 11 Nov 1996 11:40:16 GMT+1200
Subject: Pure Oxides

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Can anyone advise a source of very small quantities (yes, I'm still
in trouble with microprobe standardisation) of synthetic very pure
crystalline oxides?
I've heard that for some elements, such oxides are produced in the
semiconductor industry, but I don't have any contacts there.
My wish list would include:
MgO, Al2O3, SiO2, TiO2, V2O3 or V2O5, any Fe oxide, MnO or MnO2,
plus anything else that's available.

thanks

Ritchie

Ritchie Sims phone: 64 9 3737599 ext 7713
Department of Geology fax: 64 9 3737435
University of Auckland
Private Bag 92019
Auckland
New Zealand




From: Colin MacRae :      C.Macrae-at-minerals.csiro.au
Date: Mon, 11 Nov 1996 12:28:33 +1100
Subject: AMAS 97- Australian Microscopy Symposium and Workshops

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The Fourth Biennial Symposium on SEM Imaging and Analysis:
Applications and Techniques will be held in Melbourne, Australia
3 - 7 February 1997
Organised by Australian Microbeam Analysis Society (AMAS)

The aim of the Symposium is to provide a forum where participants can
discuss scanning electron microscopy, microanalysis,image processing and
analysis together with all their related methods and techniques. The
emphasis is on applications and techniques.

Contributions are sought on all aspects of:

SEM imaging of biological and materials specimens
X-ray analysis
Field emission SEM
Low-voltage SEM
Cryo-SEM
Digital imaging, archiving and output
Laboratory management
Training methods

Guest speakers:

Dr. David Joy, University of Tennessee, USA
Monte Carlo Simulations of Low energy electron interactions + Low Voltage
Electron Microanalysis

Dr. Val Randle, University College Swansea, UK
Electron Backscattered Kikuchi pattern analysis.

Dr. Guy Remond, BRGM Orleans, France
Light Element Analysis and specimen preparation.


Full details of the Workshops and the Symposium can be found at
http://www.minerals.csiro.au/microscopy/amas/amas.htm

For further details

Colin MacRae
Workshop co-ordinator - AMAS 97
************************************************************************
Electron Microscopy Section & Scanning Probe Microscopy

Commonwealth Scientific and Industrial
Research Organisation. _--_|\ cmac-at-minerals.csiro.au
Division of Minerals / \ Ph. 61 3 9647 0296
PO Box 124, Port Melbourne 3207 \_.--._/ Fax 61 3 9646 3223
AUSTRALIA

EM units WWW site http://www.minerals.csiro.au/em-unit/
*************************************************************************





From: ScottE57-at-aol.com
Date: Sun, 10 Nov 1996 21:59:51 -0500
Subject: Re: How to annotate tiff files?

Contents Retrieved from Microscopy Listserver Archives
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} On Nov.7th Rihcard Thrift wrote:
} There must be a way, but I'm not aware of it. Can I add a
} brief description of the sample to the tiff file, so that the
} description will always stay associated with the image?
}
} What software will allow me to read/write the description?
} in general, what software is used to edit tiff files other than
} editing the image itself?
} I'm using a PC.
}
} Thanks
} Richard

Richard, Image Central from Advanced Imaging Concepts is an MS-Windows based
image databse that will let you catalog your images with any type of
information you like then sort and query on that data, also we can associate
a tiff image overlay plane with any image and keep that as a separate file or
merge it into the original for printing only or permanently.

Contact me at : Scott E57-at-aol.com and I will gladly mail you a packge of
literature and price list for our products.

Scott E. Berman
Advanced Imaging Concepts, inc.
Phone:(609) 921-3629
Fax:(609) 924-3010
e-mail Scott E57-at-aol.com




From: Stephen Griffiths :      s.griffiths-at-ucl.ac.uk
Date: Mon, 11 Nov 1996 07:57:59 +0000
Subject: Re: TEM film price increase

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Message-Id: {1.5.4.16.19961111075759.231f5286-at-pop-server.bcc.ac.uk}
X-Sender: smgxstg-at-pop-server.bcc.ac.uk
X-Mailer: Windows Eudora Light Version 1.5.4 (16)
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

At 16:31 07/11/96 -0700, you wrote:
} I just ordered some Kodak 4489 TEM film, and was shocked by the price
} increase from my normal supplier. Our price went from about $45/100 sheets
} to $70. Has anyone else had the same surprise?
}
} I'd be interested in cheaper sources of this film or comparable EM films.
} I have been told that the Ilford EM film has been discontinued.
}
} John
} chandler-at-lamar.ColoState.EDU

****************************************************************************
**********

Unfortunately Ilford EM Film has been unavailable for some years now, a pity
because it was an excellent product. When this occured we investigated the
prices of other films.

I can only speak for UK prices, naturally, but Agfa Scientia 23D56 film was
much cheaper than Kodak. However one problem we found was that although the
Agfa film was far more sensitive and therefore required less electron
exposure of the specimen, it requires the use of a dark red filter to handle
it and far more stringent EM and darkroom illumination conditions. Some
people can find this to be a bit of a problem when loading developing racks
and fiddley film holders for the EM. We found it a real pain and eventually
went over to the more expensive Kodak, where we could use an orange/brown or
brighter red filter.

Are there any other EM films on the market apart from Kodak and Agfa? I
don't know of any.

Regards
Stephen

{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
Stephen Griffiths e-mail: s.griffiths-at-ucl.ac.uk
Visual Science Department
Institute of Ophthalmology Tel: 0171 608 6914
London EC1V 9EL UK Fax: 0171 608 6850
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}





From: DENNIS WARD :      dw0005-at-epfl2.epflbalto.org
Date: Mon, 11 Nov 1996 05:39:20 -0500 (EST)
Subject: EM Lab Design

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I have the unique opportunity to assist in the design of a new EM
lab, to include 2 SEMs and a TEM, mostly for materials applications. Is
anyone aware of references describing considerations for EM lab design? Any
other suggestions would also be appreciated.

Thank you.

Dennis Ward.




From: Richard Beanland +44 1327 356363 :      richard.beanland-at-gecm.com
Date: Mon, 11 Nov 1996 11:21:25 +0000 (GMT)
Subject: RE: Reflection electron diffraction in the TEM

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Disclose-Recipients: prohibited
"Dr. Augusto A. Morrone" {amorr-at-mse.ufl.edu}
Message-Id: {5725211111111996/A14165/CAUV40/11AB5AD50000*-at-MHS}
Autoforwarded: false
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Hop-Count: 2

Augusto,
I am not sure what you mean by an attachment to do RHEED and REM. I
did this some time ago in a Phillips 400t; all you need is an appropriate
specimen. There are some excellent papers by Tung Hsu on the subject [e.g.
Ultramicroscopy _11_, 239, (1983), J. Vac. Sci. Tech. _B3_ (1985)] There is
also an 'idiots guide', but I don't have the reference (J. Appl. Cryst 1983?)
.

Regards,

Richard Beanland,
GMMT Ltd.,
Caswell,
Towcester,
Northants NN12 8EQ
UK

} Dear Microscopy Netters:
}
} I would like to get information on reflection electron diffraction
} in the TEM. Recently, someone asked me if we had on our JEOL 200CX an
} attachment to do reflection electron diffraction. I heard this attachment
} was more common in older JEOL TEMs (JEM 7 or 9), and that it is installed in
} the column somewhere below the OL or even below the intermediate lens. Does
} anybody have this facility, or has any information about it?
}
} Augusto A. Morrone
} 107D-MEL, P.O. Box 116400
} MAIC
} Materials Science and Engineering
} University of Florida
} Gainesville, FL 32611
} (352) 392-1497 or 6985
} Fax: (352) 392-0390
} amorr-at-mse.ufl.edu





From: Warren Straszheim :      wes-at-ameslab.gov
Date: Mon, 11 Nov 1996 07:46:58 -0600
Subject: Re: m-probe, Kevex - program to convert sys75.kvx file?

Contents Retrieved from Microscopy Listserver Archives
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Kermit is definitely available for the PDP-11 running RT-11 or TSX+. We have
used it plenty on our Kevex 8000 Delta V, which also has a 10 MB bernoulli.
I can get you a copy, plus it should be available on line from any number of
places - but then there is the issue of getting it onto your machine.

You will need to determine that you have a RS-232 serial port available. And
you will need to check the settings on it. They were not as common or as
straightforward as the ones on PCs.

The file itself will be a bit of fun. I know there was an export utility
that would convert files to ASCII format one at a time, but they would
contrain only the counts and acquire time. You would have to account for the
rest: energy resolution, geometry, kV, etc. But it can be done.

BTW, what do you intend to do with the info once on the PC, i.e., what
software processing?

Give me a call or e-mail and I can give you more specifics. Or it may be
that others on the list have found better ways to transfer the info.

At 10:12 AM 11/9/96 -0800, you wrote:
} I need to move a sys75.kvx file off of a DEC lsi 1173 and onto a p.c.
} Once on the p.c., I would like to analyze the eds spectra (print out a
} spectra and extract semi-quantitative elemental composition - and I
} would like for it to be user friendly (boy, am I asking for a lot, or what!)
}
} I am praying that the DEC has a copy of kermit on it. If not, then I will
} have to either copy files one by one over the printer port to a laptop with
} kermit
} on it or someone get a copy of kermit onto the DEC.
}
} Some other information: DEC LSI 1173 running on RT11, Kevex 9000 system
} 7579 delta5.
} The DEC is connected to the outside world by 10 MB disks...
}
} Is there any hope?
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Nestor J. Zaluzec :      zaluzec-at-sparc5.microscopy.com
Date: Mon, 11 Nov 1996 08:36:17 -0500
Subject: Submitting Meeting Information and WWW Site

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Message-Id: {v03007801aeacd8c9c83c-at-[206.69.208.21]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

G'day Subscribers...

Many of you use the Microscopy Listserver to
announce meetings/workshops/short courses
which is great. However, I don't always gleen
all the information I need to easily add your
information to the Master DataBases that I try
to maintain at

http://www.amc.anl.gov/Docs/NonANL/Meetings.html

In order to help (me) I have created an electronic
submission form which you can fill out. It
will simplify my life a bit if you use it. The
URL is:

http://www.msa.microscopy.com/MSAUtilities/MeetingForm.html
or
http://www.amc.anl.gov/Docs/NonANL/MeetingForm.html

Both of these sites have the same form, one is just a mirror
of the other.

I have also created a form to register your URL with
the WWW Microscpy Site Data Base at

http://www.amc.anl.gov/#NatIntSites


This also has a new electronic form:

http://www.msa.microscopy.com/MSAUtilities/WWWForm.html
or
http://www.amc.anl.gov/Docs/NonANL/WWWForm.html

Both of these sites also have the same form, one is just a mirror
of the other.

Any and all submissions are welcome, however, they will not
get "posted" until I check them out to make sure they are
not bogus.

Cheers... Nestor
Your Friendly Neighborhood SysOp






From: ebs-at-ebsciences.com
Date: Mon, 11 Nov 1996 09:38:24 -0600
Subject: Re: Pure Oxides

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Hi Ritchie!

Some of the oxides on your list are available from us as (approx) 2 x 2mm
grains, or mounted on brass blocks. They include:

Magnesium oxide
Aluminum oxide
Silicon oxide
Ferrous oxide

Disclaimer: Energy Beam Sciences is the United States distributor for
MicroAnalysis Consultants, who manufacture standards for x-ray microanalysis.

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: ldm3-at-apollo.numis.nwu.edu (L. D. Marks)
Date: Mon, 11 Nov 1996 09:26:05 -0600
Subject: Postdoc Position

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A postdoctoral position is available, starting immediately, to work on a numb
er of different projects all involving a unique UHV-HREM (see http://risc1.nu
mis.nwu.edu). The projects are:
1) Application of some new techniques for imaging surfaces at the ato
mic scale,and directly interpreting diffraction patterns.
2) In-situ growth and characterization of cubic boron-nitride hard co
atings.
3) Characterization of high temperature superconductors.

An good background in transmission electron microscopy or STEM is required,
and a background in any of the above areas and/or thin film growth or surface
science will help, as will profiency in computer programming for UNIX computers
(Fortran, C, X-windows).

Applicants should respond by email to ldm3-at-apollo.numis.nwu.edu enclosing
copies of C.V., names of referees etc.




From: dlietz-at-trentu.ca (deborah Lietz)
Date: Mon, 11 Nov 1996 15:15:57 +0100
Subject: sem sand samples

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} Date: Wed, 06 Nov 1996 17:05:51 +0100
} From: dlietz-at-TrentU.ca (deborah Lietz)
} Subject: sem snad samples
} To: microscopy-at-msa.com
} Cc: dlietz-at-TrentU.ca
} MIME-version: 1.0
}
} I have been given sand samples containing cyanobacteria which have been
} grown on the sand. I am having problems getting the samples to stay on the
} stubs. The sand crusts are too fragile to push down onto double sided tape
} and conductive paint is just soaked up by the sand but it really doesn't
} make it adhere. Does anyone have any suggestions? Any replys would be
} greatly appreciated.
}
} Thanks Debbie
}
}
} Debbie Lietz
} Electron Microscopy Suite
} Department of Biology
} Trent University
} Peterborough, Ontario
} K9J 7B8
} Telephone: (705)748-1486
} Fax: (705) 748-1205
} email: dlietz-at-trentu.ca
}
}

Debbie Lietz
Electron Microscopy Suite
Department of Biology
Trent University
Peterborough, Ontario
K9J 7B8
Telephone: (705)748-1486
Fax: (705) 748-1205
email: dlietz-at-trentu.ca






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 11 Nov 1996 16:19:27 -0400
Subject: RE-Refl'n ED in a TEM

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Subject: Time: 4:12 PM
OFFICE MEMO RE:Refl'n ED in a TEM Date: 11/11/96

When I was getting started in the fields of ED and EM some 50 years ago,
reflection electron diffraction was one of the new and exciting techniques,
and both GE and RCA marketed instruments specifically designed for reflection
electron diffraction. I did my Ph.D. research with Prof. L. O. Brockway,
who was one of the pioneers in the field of electron diffraction. There is
a chapter which I wrote on reflection electron diffraction in Vol. I of the
second edition of the book "Physical Methods in Chemical Analysis" W. G.
Berl, Ed. Academic Press (1960). My chapter is based on the use the
dedicated instruments; I believe the corresponding chapter in the Third
Edition may include a discussion of doing reflection ED in a TEM.

In any event, section 5.3 in Vol 1 of the Series 'Practical Methods in
Electron Microscopy',. A. M. Glauert, Ed. (ISBN 0-444-10404-6) North Holland,
1972 describes reflection ED in a TEM, and even shows a picture of an
attachment used for the purpose on a Siemens TEM.





From: DENNIS WARD :      dw0005-at-epfl2.epflbalto.org
Date: Mon, 11 Nov 1996 05:39:20 -0500 (EST)
Subject: EM Lab Design

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Microscopy-at-Sparc5.Microscopy.Com (MICROSCOPY MSA list messages)
Message-ID: {1996Nov11.153500.1814.164777-at-missgate.sunderland.ac.uk}
X-Mailer: Microsoft Mail via PostalUnion/SMTP for Windows NT
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Organization: University of Sunderland


Yes
the classic reference is :
Design of the Electron Microscope Laboratory
by R.H. Alderson (Vol 4 of Practical Methods in Electron Microscopy ed
Audrey M. Glauert) 1975 (reprint 1985) pub. Elsevier, NY & Amsterdam
ISBN 0-7204-4260-5 (paperback); ISBN 0-7204-4259-1 (hardback)

but there is also a chapter in the loose leaf publication:
Procedures in Electron Microscopy (Eds A.W. Robards & A.J. Wilson) 1993-to
present
pub. John Wiley & Sons (Chichester, NY, Brisbane, Toronto, Singapore)
ISBN 0 471 92853 4
-Chapter 1 Setting up an Electron Microscope Facility (pp 1:1.1-1:1.23;
1:2.1-1:2.2)

There also seem to be little bits/chapters in various other texts eg:
Practical Electron Microscopy for Biologists - Geoffrey A. Meek 2nd Edn
reprint 1981
Pub John Wiley (Chichester, NY, Brisbane, Toronto)
ISBN 0 471 59031 2 (cloth); ISBN 0 471 99592 4 (paper)
Chapter 9 Commercial T.E.M.s (bit out of date) Chapter 10 Installation

and of course consult the manufacturers' literature which should give a lot
of information about ambient temperatures, vibration, magnetic fields, power
requirements and size of rooms

I hope some of this helps

Malcolm Haswell
University of Sunderland
UK

----------

I have the unique opportunity to assist in the design of a new EM
lab, to include 2 SEMs and a TEM, mostly for materials applications. Is
anyone aware of references describing considerations for EM lab design? Any

other suggestions would also be appreciated.

Thank you.

Dennis Ward.

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From: Bill Chissoe :      wchiss-at-ou.edu
Date: Mon, 11 Nov 1996 14:18:04 -0600
Subject: LaB6

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Message-ID: {328789FC.7675-at-ou.edu}

We have an instrument in our lab that has a LaB6 source and I recently
had to replace it. Anybody that has had to clean evaporated LaB6 off the
inside of their wehnelt can identify with my situation--it's doggone
hard on the fingers!!! Our service rep told me about a procedure that
uses HNO3 (nitic acid) but the instructions were unclear and seemed
incomplete. On top of that, he could not recall where his information
had come from. Is anybody familiar with this procedure or can you
possibly shed some light this subject? Thanks.

Bill
--
=============================================================
Bill Chissoe III
Electron Microscopist,University of Oklahoma
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================




From: dana nojima :      noji-at-lenti.med.umn.edu
Date: Mon, 11 Nov 1996 19:40:05
Subject: RE: High Resolution networked Printers

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Message-Id: {9611112341.AA26848-at-lenti.med.umn.edu}
To: mxkalyan-at-pau.mobil.com, Microscopy-at-Sparc5.Microscopy.Com

} Our company is currently looking to buy a printer for high
} quality output of micrographs. Also, it would be have to be
} easily networkable.
} Is a 1200X1200 dpi printer good enough? Are there other criteria
} I should apply?

If you are looking at only dpi, these printers give good resolution. The
problem is that they have no gray scale unless you change the screen size.
This will reduce the resolution in ralationship to the desired amount of
gray scale desired.

Dana Nojima
noji-at-snowman.med.umn.edu __~0_\___
Minneapolis, Minnesota (_________)
(612) 624-4687 O O







From: McCaffrey, John :      John.McCaffrey-at-nrc.ca
Date: Mon, 11 Nov 1996 12:17:00 -0500
Subject: Re: TEM image simulation

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Message-ID: {c=CA%a=_%p=NRC%l=NRC/IMSD/0004C975-at-imsd-exchange.nrc.ca}
worldwide BB for microscopist {microscopy-at-Sparc5.Microscopy.Com}

Hello Richard,
If the information that you require is the alloy concentrations in
AlGaAs, InGaAs, etc., there is another fairly simple way to measure
this. We
recently had a paper accepted (Phil. Mag. A) that makes use of the
difference
in the diffracted intensities between GaAs, In(x)Ga(1-x)As and
Al(y)Ga(1-y)As
films using (200) dark field conditions to determine the alloy content
of
these layers. Essentially, we derived the total In or Al content in the
structure from a DCXRD measurement, then acquired accurate contast
profiles
of the individual layers using dark field techniques. This approach can
*probably* be applied in InGaP and other ternaries and quaternaries,
although
we've only done the dynamical calculations for InGaAs and AlGaAs so far.

The most interesting result was that simple structure factor
calculations
almost exactly matched the more difficult dynamical theory calculations
for
the questions "where is the indium or aluminum in the layers, and how
much of
it is there?" In the AlGaAs case, this was pointed out twenty years ago
by
Bithell and Stobbs.
Anyway, I've added the abstract at the end of this note - if you'd
like a
preprint, please let me know.

Cheers
John
-----------------------------------------------------------------------
-
| John P. McCaffrey tel: Canada 613-993-7823 |
| National Research Council of Canada fax: Canada 613-990-0202 |
| Inst. for Microstructural Sciences _____ _____ |
| Montreal Road Labs, Bldg. M-50 | | __/\__ | | |
| Ottawa, Ontario, K1A 0R6 | | __/\\ //\__ | | |
| Canada | | \ \\ // / | | |
| | | /___ ___\ | | |
| email: john.mccaffrey-at-nrc.ca | | /__ __\ | | |
| | | || | | |
| |_____| |_____| |
-----------------------------------------------------------------------
-
ABSTRACT
During the production of InxGa1-xAs quantum wells by the crystal growth
technique of molecular beam epitaxy (MBE), the indium tends to
accumulate in
the surface layer and float on the crystal growth surface. This effect
delays incorporation of the indium into the quantum well and results in
a
graduated value of "x" across the well. Detailed indium concentration
profile information is essential if accurate theoretical models are to
be
applied to describe these quantum wells. Therefore, to quantify this
indium
segregation we combine the complimentary results of two experimental
techniques, transmission electron microscopy (TEM) and double crystal
x-ray
diffraction (DCXRD). The indium concentration well profiles were
determined
through an analysis of the contrast variation in (200) dark field TEM
images. Differences of less than 0.5% indium concentration were
discernible.
The DCXRD analysis provided an accurate value for the total amount of
indium
in the individual wells which was used as the scaling factor for the TEM
concentration profile. To insure that the observed contrast differences
were
due to variations in indium concentration and were not merely TEM sample
preparation artifacts, high quality cross-sectional TEM samples with
parallel
cleaved surfaces free of ion milling damage were required. Accurate
thickness values for the samples were also needed. A TEM sample
preparation
technique was developed to provide for these requirements.
Indium concentration profiles were taken from intensity line scans
across
dark field TEM images of these samples. These profiles were interpreted
by a
simple kinematical theory (structure factor) calculation, and then by a
more
rigorous model using the dynamical theory of electron diffraction. The
results of these two models were then checked for consistency with the
results of the DCXRD measurement. The model profiles of indium
segregation
within the quantum wells calculated using the structure factor approach
for
up to 35% indium content, matched very closely the profiles produced by
the
use of dynamical theory. Both sets of model profiles strongly supported
by
the DCXRD results. These results demonstrate that a simple structure
factor
calculation in conjunction with a DCXRD measurement can allow an
accurate
measure of quantum well profiles and of the abruptness of InxGa1-xAs on
GaAs
and GaAs on InxGa1-xAs interfaces. This information is a powerful aid
in the
modeling and understanding of devices based on these structures.




From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Tue, 12 Nov 1996 11:16:18 +1100 (EST)
Subject: Re: sem sand samples

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} }
} } I have been given sand samples containing cyanobacteria which have been
} } grown on the sand. I am having problems getting the samples to stay on the
} } stubs. The sand crusts are too fragile to push down onto double sided tape
} } and conductive paint is just soaked up by the sand but it really doesn't
} } make it adhere. Does anyone have any suggestions? Any replys would be
} } greatly appreciated.


Tempfix is handy for this sort of sample. Warm up your stubs to 80 deg C
or so and smear a thin film of tempfix (SPI Cat #5058-AB, page 47) on the
warm stub. You can make several and cool them for later use or use them
while warm. The warm tempfix adheres to the sand (or powder) but wont wet
it. I suspect tempfix is very like the glue for hot glue guns any hardware
store sells.

Mel Dickson





From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Mon, 11 Nov 1996 15:47:04 -0600
Subject: Re: sem sand samples

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Message-Id: {199611112145.PAA16256-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} } I have been given sand samples containing cyanobacteria which have been
} } grown on the sand. I am having problems getting the samples to stay on the
} } stubs. The sand crusts are too fragile to push down onto double sided tape
} } and conductive paint is just soaked up by the sand but it really doesn't
} } make it adhere. Does anyone have any suggestions? Any replys would be
} } greatly appreciated.
} }
} } Thanks Debbie
} }
} }
} } Debbie Lietz

Have you tried Pella's double sticky carbon discs? They're a bit
sticky than some tapes. You might also try exposing the tape to acetone
vapor before dropping on the sand. The vapor will slightly dissolve the
glue and make the sand stick better. Maybe.
Lightly tapping the bottowm of the stub on a counter will help
also, driving the sand into the glue.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel *all mail:*
Microscopy
University of Illinois Station A
Rm 74 Bevier Hall PO Box 5037
905 S. Goodwin Ave. Champaign, IL 61825-5037 USA
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu
(217) 355-1143 home

*********** looking for a job again ***********






From: Stuart McKernan :      mckernan-at-cems.umn.edu
Date: Mon, 11 Nov 1996 17:41:16 -0600
Subject: Re: sem sand samples

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Responding to the message of {01IBQ0R7WCKY007YL9-at-TrentU.ca}
from dlietz-at-trentu.ca (deborah Lietz):
} } I have been given sand samples containing cyanobacteria which have been
} } grown on the sand. I am having problems getting the samples to stay on the
} } stubs. The sand crusts are too fragile to push down onto double sided tape
} } and conductive paint is just soaked up by the sand but it really doesn't
} } make it adhere. Does anyone have any suggestions? Any replys would be
} } greatly appreciated.
} }

We have been able to image bacteria on wet sand by just placing the sand into
the Peltier stage and imaging in the Environmental SEM. The samples do not need
any further handling (no coating for example) and the Peltier stage allows us to
examine them in the fully hydrated state.

If you do not have access to an ESEM try Chris Gilpin at the University of
Manchester (email cgilpin-at-man.ac.uk)

Good luck

Stuart McKernan stuartm-at-maroon.tc.umn.edu
CIE Microscopy Facility, University of Minnesota Office: (612) 626-7942
100 Union Street S. E., Minneapolis, MN 55455 Lab: (612) 624-6590





From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Mon, 11 Nov 1996 14:08:54 -0600
Subject: Re: High Resolution networked Printers

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Mime-Version: 1.0
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I wouldn't bother with laser printers. Get yourselves a good dye
sublimation printer. I recommend the Codonics NP1600. It has a
Sparcstation built into it, it can be networked, you can ftp files to it,
you can set up folders for several users, it will automatically size your
output to match the printing area, etc. It is hard to beat it in quality
and price. You'll be disappointed if you want high quality output but only
have a laser printer. We have 1200 dpi laser printers, but we use them
only for cheap, low-quality prints.

} Hi,
} Our company is currently looking to buy a printer for high
} quality output of micrographs. Also, it would be have to be
} easily networkable.
} Is a 1200X1200 dpi printer good enough? Are there other criteria
} I should apply?
} If you have any experience with such printers, please send me an
} e-mail. Also, if there is a current archived discussion (these
} run out of date quite quickly!) I can access it would be useful.
} Thank you.
}
} Mohan Kalyanaraman
} Mobil Technology Company
} 609-224-3989 (ph#)
} mxkalyan-at-pau.mobil.com

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(630)252-7194
FAX: (630)252-4798






From: Woody.N.White-at-mcdermott.com
Date: 11/9/96 12:12 PM
Subject: m-probe, Kevex - program to convert sys75.kvx file?

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Not precisely the same, but... I have a Kevex 8005 (8000 box,
upgraded to the point of being almost a Delta). I can save/externally
the data as ascii. I then can use my "sneaker-net" to get data to a
PC. I have the program "RTDIR" and RTCOPY which, along with a 44 Mb
Bernoulli and SCSI card in a PC, will permit cross-platform copies to
be made.

Woody White


______________________________ Reply Separator _________________________________


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I need to move a sys75.kvx file off of a DEC lsi 1173 and onto a p.c.
Once on the p.c., ...SNIP...

Steve

***************************************
Dr. Steven S. Trail
B.P. Chemicals
4440 Warrensville Center Rd
Warrensville Hts., OH 44128
(216) 586-5136 (work)
(216) 586-5030 (fax)
trailss-at-bp.com
trail-at-cyberdrive.net (home)
***************************************




From: Yi_Feng-at-pei.philips.com (Yi Feng)
Date: 11/11/96 11:21 AM
Subject: RE: Reflection electron diffraction in the TEM

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Mime-Version: 1.0
Richard Beanland +44 1327 356363 {richard.beanland-at-gecm.com}
Content-Type: text/plain; charset=US-ASCII
Content-Transfer-Encoding: 7bit
Content-Description: cc:Mail note part

Augusto,

Philips has a special bulk sample holder for reflection diffraction
applications (called reflection diffraction holder). It allows you to put a
bulk piece of sample onto the stage with the sample surface parallel to the
electron beam at zero tilt. You can tilt the holder a few degree to obtain
a glancing angle in order to obtain reflection diffractions. You can also
rotate the stage to obtain diffractions from different orientations.
If your sample is small enough, you can modify a double tilt holder to do
certain reflection work (see also Tung Hsu's paper: Technique of reflection
electron microscopy published in Microscopy Research and Technique
20:318-332, 1992). The thing is that you need to mount your speciman
surface to be diffracted nearly parallel to the beam.

Regards,

*******************************
Yi Feng, Ph.D.
Sr. Applications Specialist
Philips Electronic Instruments
85 McKee Drive
Mahwah, NJ 07430 USA

E-mail: yi_feng-at-pei.philips.com
Tel: 201 529 6405
Fax: 201 529 2252
*******************************








______________________________ Reply Separator _________________________________


Augusto,
I am not sure what you mean by an attachment to do RHEED and REM. I
did this some time ago in a Phillips 400t; all you need is an appropriate
specimen. There are some excellent papers by Tung Hsu on the subject [e.g.
Ultramicroscopy _11_, 239, (1983), J. Vac. Sci. Tech. _B3_ (1985)] There is
also an 'idiots guide', but I don't have the reference (J. Appl. Cryst 1983?)
.

Regards,

Richard Beanland,
GMMT Ltd.,
Caswell,
Towcester,
Northants NN12 8EQ
UK

} Dear Microscopy Netters:
}
} I would like to get information on reflection electron diffraction
} in the TEM. Recently, someone asked me if we had on our JEOL 200CX an
} attachment to do reflection electron diffraction. I heard this attachment
} was more common in older JEOL TEMs (JEM 7 or 9), and that it is installed in
} the column somewhere below the OL or even below the intermediate lens. Does
} anybody have this facility, or has any information about it?
}
} Augusto A. Morrone
} 107D-MEL, P.O. Box 116400
} MAIC
} Materials Science and Engineering
} University of Florida
} Gainesville, FL 32611
} (352) 392-1497 or 6985
} Fax: (352) 392-0390
} amorr-at-mse.ufl.edu





From: Bill Chissoe :      wchiss-at-ou.edu
Date: Mon, 11 Nov 1996 14:18:04 -0600
Subject: LaB6

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Message-ID: {n1364377501.88381-at-mse.engin.umich.edu}

Bill
--
=============================================================
Bill Chissoe III
Electron Microscopist,University of Oklahoma
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================

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From: unit-at-wolsi.com
Date: Mon, 11 Nov 1996 12:21:39 -0700
Subject: Request to buy ad-space on your page.

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Hello. My name is Aron Hsiao; I'm contacting you on behalf of Access
Development Corporation and MemberWeb which resides at
http://www.memberweb.com on the World-Wide Web.

We have found your e-mail address on the World-Wide Web and understand
you may own a business or information-related World-Wide Web page. We
want to buy advertising space on this page. We pay by commission (for
users which click our advertisement on your page, thereby visiting our
site). Details and specific advertisement information are at
http://www.wolsi.com/~cghsiao/JnA/aron/memberweb.html along with a
form which can be filled out to let us know you are interested in
selling space to MemberWeb.

For details on our advertising program via e-mail, please mail to
unit-at-wolsi.com with a request for information. E-mailing us does
/not/, I repeat, /not/ obligate you to sell advertising space to us.

If you feel that you have received this message in error, please
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From: Stephen Griffiths :      s.griffiths-at-ucl.ac.uk
Date: Mon, 11 Nov 1996 16:30:48 +0000
Subject: Re: EM Lab Design

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At 05:39 11/11/96 -0500, Dennis Ward wrote:
} I have the unique opportunity to assist in the design of a new EM
} lab, to include 2 SEMs and a TEM, mostly for materials applications. Is
} anyone aware of references describing considerations for EM lab design? Any
} other suggestions would also be appreciated.
}
} Thank you.
}
} Dennis Ward.
****************************************************************************
*****
If it's still in print try :-

Practical Methods in Electron Microscopy ed. Audrey Glauert
Volume 4:
Design of the Electron Microscope Laboratory
by R.H. Alderson
ISBN for the series is 0 7204 42508

Sorry, I don't have the book to hand for the individual ISBN.

It was written in 1975 but should not be too dated for most general design
requirements

Regards
Stephen Griffiths


{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}
Stephen Griffiths e-mail: s.griffiths-at-ucl.ac.uk
Visual Science Department
Institute of Ophthalmology Tel: 0171 608 6914
London EC1V 9EL UK Fax: 0171 608 6850
{} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {}





From: Jim Darley :      p&s-at-ultra.net.au
Date: Tue, 12 Nov 1996 21:15:01 +1100
Subject: LaB6; cleaning Wehnelts

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Message-Id: {1.5.4.32.19961112101501.0067fbb4-at-mailhost.ultra.net.au}
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} Date: Mon, 11 Nov 1996 14:18:04 -0600
} From: Bill Chissoe {wchiss-at-ou.edu}
} Reply-To: wchiss-at-ou.edu
} Organization: University of Oklahoma
} To: Microcoscpy List Server {microscopy-at-Sparc5.Microscopy.Com}
} Subject: LaB6
}
} We have an instrument in our lab that has a LaB6 source and I recently
} had to replace it. Anybody that has had to clean evaporated LaB6 off the
} inside of their wehnelt can identify with my situation--it's doggone
} hard on the fingers!!! Our service rep told me about a procedure that
} uses HNO3 (nitic acid) but the instructions were unclear and seemed
} incomplete. On top of that, he could not recall where his information
} had come from. Is anybody familiar with this procedure or can you
} possibly shed some light this subject? Thanks.
}
} Bill
} --
} =============================================================
} Bill Chissoe III
} Electron Microscopist,University of Oklahoma
} E-mail: wchiss-at-ou.edu Ph. (405)325-4391
} ==========================================================
Bill & other EMers:
Use ammonia, not nitric acid! Just place the Wehnelt
in a beaker with some ammonia, leave it for an hour or over-night. Cover the
top and use a fumehood. Most of the deposit will just float off. Light
polishing with Wehnol or Pol is only sometimes required. This has to be
removed with solvents and another shorter period in ammonia followed by a
water rinse and drying. Very little "elbow grease" is required.
Do not use ammonia on copper or brass parts.
Do not use metal polish in pole piece bores.
Cheers
Jim Darley

PS Recent changes to our site discriminated against Netscape II users.
That's fixed. Enjoy!
Probing & Structure
} (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia
}
} Phone +61 77 740 370 Fax: +61 77 892 313
} A great microscopy site http://www.ultra.net.au/~pns/





From: sdw-at-biotech.ufl.edu (Scott Whittaker)
Date: Tue, 12 Nov 1996 08:03:52 -0500 (EST)
Subject: Re: sem sand samples

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There is a product from Electron Microscopy Sciences called "Temp
Fix". Basically you coat some stubs with a layer of it and when you are
ready with the samples, sprinkle some on the stub. Put the stub in the oven
(60C) or so and it will melt and adhere the samples. Let me know if I can
assist you further.




} } I have been given sand samples containing cyanobacteria which have been
} } grown on the sand. I am having problems getting the samples to stay on the
} } stubs. The sand crusts are too fragile to push down onto double sided tape
} } and conductive paint is just soaked up by the sand but it really doesn't
} } make it adhere. Does anyone have any suggestions? Any replys would be
} } greatly appreciated.
} }
} } Thanks Debbie
} }
} }
} } Debbie Lietz
} } Electron Microscopy Suite
} } Department of Biology
} } Trent University
} } Peterborough, Ontario
} } K9J 7B8
} } Telephone: (705)748-1486
} } Fax: (705) 748-1205
} } email: dlietz-at-trentu.ca
} }
} }
}
} Debbie Lietz
} Electron Microscopy Suite
} Department of Biology
} Trent University
} Peterborough, Ontario
} K9J 7B8
} Telephone: (705)748-1486
} Fax: (705) 748-1205
} email: dlietz-at-trentu.ca
}
}
}
}



} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {


Scott Whittaker ph: 352-392-1184

Research Assistant fax: 352-846-0251
ICBR EM Core Lab e-mail: sdw-at-biotech.ufl.edu
University of Florida http://www.biotech.ufl.edu/~emcl/





From: dlietz-at-trentu.ca (deborah Lietz)
Date: Tue, 12 Nov 1996 10:22:50 +0100
Subject: thanks (sem sand)

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Thank everyone who has replied to my question about mounting sand
for sem. I now have a lot of different things to try and I'm sure one will
work.


Thank-you

Debbie Lietz

Debbie Lietz
Electron Microscopy Suite
Department of Biology
Trent University
Peterborough, Ontario
K9J 7B8
Telephone: (705)748-1486
Fax: (705) 748-1205
email: dlietz-at-trentu.ca






From: Mark Wall :      Mark.Wall-at-quickmail.llnl.gov
Date: 12 Nov 1996 07:51:31 -0800
Subject: Mark Wall

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Message-ID: {n1364337300.60430-at-quickmail.llnl.gov}

Looking for a Side Entry Goniometer (SEG) for a JEOL 200/100 A or B or CX etc.
Preferably something to be given away from an unwanted TEM. (We don't need the
TEM though!)

Thanks

Mark Wall
Lawrence Livermore National Lab
510 423-7162





From: clsmteam-at-imiucca.csi.unimi.it
Date: Tue, 12 Nov 1996 17:49:32 GMT
Subject: Buffer for achetylcholinesterase

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A collegue of mine asks:


To whom it may concern:

I working on neurovascular control in the spinal cord.
I would like to receive some informations on the Karnovsky and Roots
method for Achetylcholinesterase enzymatic activity detection. In
particular I would
like to know which buffer is more suited for the incubation medium.

Thank you


Dr. Cristiano Rumio
Istitute of Anatomy
University of Milan
Via Mangiagalli 31, 20133 Milan
Italy
E-mail clsmteam-at-imiucca.csi.unimi.it
Voice: -39.2.2663683
Fax:-39.2.2364082





From: Murat AYDIN :      muratay-at-pamuk.cc.cu.edu.tr
Date: Tue, 12 Nov 1996 17:57:32 +0300 (EET)
Subject: TEM - Need help on Candida cells

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Dear all,
I need help about comment of Candida albicans TEM photographs.
I think, there are many experts in the list, I will be glad if one of
them can help me about comment. I can send the 5 photos to a familiar one(s=
)
with E-mail in TIF, PCX, BMP or any format.

Sincerely.
Murat AYDIN
muratay-at-pamuk.cc.cu.edu.tr

-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=
=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D
Background information:
Candida albicans cells were exposed to 15 =E6A of anodic direct
current with silver electrodes in a culture medium for 48 hours. Yeast
colonies nearby the anode and their controls were examined in TEM.
Samples were separately introduced into two 5% glutaraldehyde
solutions (5 ml; pH, 7.4). Fixed cells were washed twice with 0.05 M
phosphate buffer 0.8 M sorbitol, then postfixed in 0.5% OsO4 overnight
at 4 degree, washed in distilled water, dehydrated through an ethanol
series, embedded in resin, after polymerization, ultrathin sections were
collected on grids, stained with 7% uranyl acetate and with lead citrate.

=09There are many intra-cytoplasmic vesicles in the Candida cells,
but which is what ? and what happened in the cells ?
-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=
=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D-=3D





From: Augusto A. Morrone :      amorr-at-mse.ufl.edu
Date: Tue, 12 Nov 1996 14:27:03 -0500
Subject: REM-RHEED/ Will Bigelow

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Dear Netters:
Thank you for all the responses to my question about holders to perform
REM-RHEED in the TEM. If anyone is interested I can send a summary of
responses.

Will Bigelow: Sorry I lost your message, and I don't have your address.
Please resubmit the message if you still have (I did read it before messing
up, I particularly enjoyed the historical information!)

Cheers,
Augusto
Augusto A. Morrone
107D-MEL, P.O. Box 116400
MAIC
Materials Science and Engineering
University of Florida
Gainesville, FL 32611
(352) 392-1497 or 6985
Fax: (352) 392-0390
amorr-at-mse.ufl.edu





From: GANTZ-at-med-biophd.bu.edu
Date: Tue, 12 Nov 1996 14:06:45 -0400 (EDT)
Subject: LaB6: Wehnelt Aperture Cleaning

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From: GANTZ-at-med-biophd.bu.edu
Date: Tue, 12 Nov 1996 14:06:45 -0400 (EDT)
Subject: LaB6: Wehnelt Aperture Cleaning

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From: GANTZ-at-med-biophd.bu.edu
Date: Tue, 12 Nov 1996 14:06:45 -0400 (EDT)
Subject: LaB6: Wehnelt Aperture Cleaning

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From: GANTZ-at-med-biophd.bu.edu
Date: Tue, 12 Nov 1996 14:06:45 -0400 (EDT)
Subject: LaB6: Wehnelt Aperture Cleaning

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From: GANTZ-at-med-biophd.bu.edu
Date: Tue, 12 Nov 1996 14:06:45 -0400 (EDT)
Subject: LaB6: Wehnelt Aperture Cleaning

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Dear Bill:
I would add two comments to what has already been said
about effective removal of deposits.
1) The less muscle required to remove deposits the better
because heavy pressure might bend the aperture.

2) Six micron diamond paste is very effective in removing
oxides; somewhat less effective on those purple crystal deposits.
A little "dab will do ya" applied gently with a Q-tip followed by
rinses with dilute HCl (1:5 w/H2O), mild alkaline to neutralize and/or
water, and usual solvent such as freon. This paste is available
through Valley Design Corporation (508-692-9549); no commercial
interest motivation. Obtained this procedure from friendly people
at Kimball Physics.

Don Gantz
Boston Univ Med School
gantz-at-med-biophd.bu.edu




From: psic-at-uclink4.berkeley.edu (Paula Sicurello)
Date: Tue, 12 Nov 1996 15:40:20 -0800 (PST)
Subject: Ultracut E at Duke

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Hello!

A week ago or so someone from Duke(?) said they might sell an Ultracut E.
We're interested in buying it if it's not too expensive. Will the person
who posted that note please contact me?

Thanks!

Paula Sicurello = )
U.C. Berkeley
Electron Microscope Lab
psic-at-uclink4.berkeley.edu






From: MIchael D. Warfield :      mdwarfield-at-CCGATE.HAC.COM
Date: Tue, 12 Nov 1996 12:10:26 -0800
Subject: Variable Pressure SEM

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Message-ID: {3288D9B2.7A0B-at-ccgate.hac.com}

I am looking for reference material about application work being done
using the new variable pressure sems. Can you point me to any specific
references of libraries that might have such materials.

Mike Warfield




From: Larry Ackerman :      mishot-at-itsa.ucsf.edu
Date: Tue, 12 Nov 1996 11:26:49 -0800
Subject: Re: High Resolution networked Printers

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Message-Id: {199611121940.LAA60320-at-itsa.ucsf.edu}
X-Sender: mishot-at-itsa.ucsf.edu
X-Mailer: Windows Eudora Light Version 1.5.2
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} }
BEWARE of the Codonics dye sub printer.It does not adhere to ICC standards
for color management and the company has refused to provide a device profile
for use by other software. Virtually all other printers incorporate ICC
color management capabilities (see Kodak & Tektronix. But if you do not care
to match the image on your monitor with what you get on a print or from
batch to batch of paper or off color, ie. green & white "black & white"
images the NP-1600 can be useful. Do not purchase the on-site service and
warranty--in our case they refused to honor the contract. I could write more
but you get the idea--one of our most troublesome purchases was the Np-1600.
Larry D. Ackerman (415) 476-8751
Howard Hughes Medical Institute FAX (415) 476-5774
UCSF, Box 0724, Rm U426
533 Parnassus Ave. mishot-at-itsa.ucsf.edu
San Francisco, CA 94143





From: WARRENJ1-at-cliffy.polaroid.com
Date: 11/11/96 11:21 AM
Subject: High Resolution networked Printers

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While resolution is an important factor, the other factor that gives
substance to the resolution and grayscale levels that a imaging system
can produce is the modulation transfer function. In simplest terms,
this value, expressed as a percentage, provides you with a idea of how
accurate the produced(printed) image is when compared on a pixel by
pixel basis to the original digital file.

When considering a imaging system, you have to consider what level of
accuracy your work requires. While a dye sublimation printer gives you
a nice smooth image, it may only have a MTF of 60-65% or less in
terms of the gray scale values and the 'edges' that it is
representing. A laser print may only have 40-50% MTF. There are
applications where a thermal print would be suitable and there are
applications where a _true_ photographic quality image is required.
You did not indicate if your requirement was for color or just black
and white(grayscale). If color is a requirement, then dye sub or d2t2
technology is your best solution. Take a file and give it to the
various vendors and have them print it on their units. Be aware that
the heads on these units generally begin to wear soon and image
quality drops accordingly. If you only need gray scale, then the best
solution is a true photographic quality system that reproduces your
digital files with complete accuracy.

Who offers such a thing? Ok, I set this up. We do. The Polaroid Helios
Imaging System offers true photographic quality with 100% MTF. IT is a
fully networkable printer that can work simultaneously on Mac, IBM and
Unix platforms.


______________________________ Reply Separator _________________________________


Hi,
Our company is currently looking to buy a printer for high
quality output of micrographs. Also, it would be have to be
easily networkable.
Is a 1200X1200 dpi printer good enough? Are there other criteria
I should apply?
If you have any experience with such printers, please send me an
e-mail. Also, if there is a current archived discussion (these
run out of date quite quickly!) I can access it would be useful.
Thank you.

Mohan Kalyanaraman
Mobil Technology Company
609-224-3989 (ph#)
mxkalyan-at-pau.mobil.com




From: Donald Lovett :      lovett-at-tcnj.edu
Date: Wed, 13 Nov 1996 00:04:51 -0500 (EST)
Subject: Re: sem sand samples

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With regards to affixing sand samples to your SEM stub, try Avery's
Spot-O-Glue. It is available in most stationary stores and sounds like
exactly what you need.

Good luck.

______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
The College of New Jersey * fax: (609) 771-2674
Trenton, NJ 08650-4700

(* formerly Trenton State College; please note our new name)





From: Woody.N.White-at-mcdermott.com
Date: 11/12/96 8:04 AM
Subject: Re: m-probe, Kevex - program to convert sys75.kvx file?

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Hello Carl,

Aren't sys75.kvx library files a pain in the butt!!!!!!!
...Along with the total lack of decent (or any?) utilities, especially
for file management. Oh well, we use what we have...

The RTCOPY and RTDIR pgms were a portion of "Report Manager" which I
purchased from Kevex several years back. I may be able to send you a
copy, but under the circumstances, I must first check with Kevex.
...Really don't want to upset them or my employer. Since those pgms
are so old, they *should not* object, but.... You never know when it
comes to Kevex and $$$$.

To make the copies, you will need a Bernoulli/SCSI (like Adaptec
AHA-1542B) in the PC. If you don't have a spare Bernoulli (for PC),
maybe you could "quietly" borrow one from the EDS for the transfer. I
have only tried this with the 44Mb disk format and don't know if the
pgms will accomodate other sizes.

I have all the data needed to set-up the card which I can send to you
if you like. ---Couple of paper pages, not on disk.

The programs are a little syntex sensitive (you must enter the entire
command line, both source and destination drives, full file names,
etc.), but they operate essentially like the DOS copy and dir
commands. The PC Bernoulli is DL2: and DL6:, just as it is for the
EDS. BTW, as I recall (been a while), wild cards do work.

Regards,

Woody White, Electron Microscopist, Babcock & Wilcox Research Center
woody.n.white-at-mcdermott.com
Home:
woody.white-at-worldnet.att.net
-or-
http://www.geocities.com/capecanaveral/3722



______________________________ Reply Separator _________________________________


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Woody,

Where can I find RTDIR and RTCOPY? I've got some 8" Bernoullis on a
couple 8000s which I'd like to read on a PC or Mac.

Thanks,
Carl


On 11 Nov 1996 Woody.N.White-at-mcdermott.com wrote:

: Not precisely the same, but... I have a Kevex 8005 (8000 box,
: upgraded to the point of being almost a Delta). I can save/externally
: the data as ascii. I then can use my "sneaker-net" to get data to a
: PC. I have the program "RTDIR" and RTCOPY which, along with a 44 Mb
: Bernoulli and SCSI card in a PC, will permit cross-platform copies to
: be made.
:
: Woody White
:
:
: ______________________________ Reply Separator
_________________________________
: Subject: m-probe, Kevex - program to convert sys75.kvx file?
: Author: trail-at-cyberdrive.net_at_internet at x400post
: Date: 11/9/96 12:12 PM
:
:
: X-Sender: trail-at-pop.cyberdrive.net
: X-Mailer: Windows Eudora Pro Version 2.2 (32)
: Mime-Version: 1.0
: Content-Type: text/plain; charset="us-ascii"
:
: I need to move a sys75.kvx file off of a DEC lsi 1173 and onto a p.c.
: Once on the p.c., ...SNIP...
:
: Steve
:
: ***************************************
: Dr. Steven S. Trail
: B.P. Chemicals
: 4440 Warrensville Center Rd
: Warrensville Hts., OH 44128
: (216) 586-5136 (work)
: (216) 586-5030 (fax)
: trailss-at-bp.com
: trail-at-cyberdrive.net (home)
: ***************************************
:

Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2005 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
--------------------------------
(313) 936-1550 (voice)
(313) 763-4690 (FAX)
chender-at-umich.edu (e-mail)
--------------------------------




From: Leon Smuts :      PLBLS-at-puknet.puk.ac.za
Date: Wed, 13 Nov 1996 08:31:30 +0200
Subject: Re: m-probe, Kevex - program to convert sys75.kvx file? -Reply

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Message-Id: {s289877f.048-at-pukpcm.puk.ac.za}
X-Mailer: Novell GroupWise 4.1

} } } Warren Straszheim {wes-at-ameslab.gov} 11/11/96, 03:46pm } } }
} Kermit is definitely available for the PDP-11 running RT-11 or TSX+.
} ....
} I can get you a copy, plus it should be available on line from any
} ...
} Give me a call or e-mail and I can give you more specifics. Or...

Indeed would I also like to obtain a copy of Kermit for the PDP-11
running RT-11, and am I going to email the above person.

At this moment I am ASCII downloading data from my JEOL 733 mprobe's
PDP11, serialport to PC emulating DEC VT102 terminal (MS win3.1 also
have such an emulator built in)

Does anybody perhaps have XMODEM (or Y or Z modem) transfer protocol
source code in FORTRAN IV (fortran four) ? It is for the same purpose
of transferring binary files between PDP11 and other platforms.

I uploaded xmodem Fortran VII (seven) source to the PDP11 via the VT102
emulation as ASCII and did try compile it with the RT11's compiler
resulting in an infinite list of errors.

Thank you so much, Leon Smuts.

} \\\\\\\\\\\\\\\\} URL: http://www.puk.ac.za } WWW} \\\\\\\\\\\\\\\\\\\}
} Leon Smuts-Electronmicroprobe-University of Potchefstroom-S_Africa}
} /////////////} mailto: plbls-at-puknet.puk.ac.za } eMail} ///////////////}






From: Giorgio Gasparotto :      gaspar-at-geomin.unibo.it
Date: Wed, 13 Nov 1996 08:34:25 +0000
Subject: EDS software

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Message-Id: {199611130734.IAA08404-at-dogon.geomin.unibo.it}
Comments: Authenticated sender is {gaspar-at-geomin.unibo.it}

Hi,

I need help with EDS analyses. I am searching software and /or references
about quantitative EDS analyses of minerals. What I need is not
general literature that I know well but specific literature (or
software, source code ecc) about for instance background modeling,
intensity calculation and so on. I am currently working with an EDS system
which has a software package absolutely unsatisfactory expecially in background
modeling.
Does exist software (commercial or free) that read data in ascii format
(eV vs Intensity) or proprietary format and perform quantitative calculations
with standards?
About the make of the EDS system I prefere to talk about it only in
private e-mail.
Best regards

-------------------------------------------
Giorgio Gasparotto
Dipartimento di Scienze della Terra
e Geo-Ambientali
Piazza di Porta S. Donato 1
40126 Bologna Italy
Tel. 51 243.556 FAX 51 243.336
WWW: http://geode.geomin.unibo.it/min/
Internet e-mail gaspar-at-geomin.unibo.it
-------------------------------------------




From: timon.fliervoet-at-uni-bayreuth.de (Timon Fliervoet)
Date: Wed, 13 Nov 1996 16:14:55 +0100
Subject: TEM: EDS software-2

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Message-Id: {199611130910.JAA1607390-at-f01n05.cac.psu.edu}

Dear all,

To follow up on Giorgio's request on EDS software. Does any of you know
software (comercial/freeware - PC/Mac/SUN) capable of doing off-line
absorption corrections, K-factor analysis etc with ascii net-intensity
data. We are using a Noran Voyager system attached to our TEM and would
like to do the analysis off-line. Thereby not using up TEM time, also I
don't like to way Noran saves my data afterwards.

Any help and suggestions are greatly appreciated.

Cheers, Timon

---------------------
Timon Fliervoet
Bayerisches Geoinstitut, Universitat Bayreuth
D-95440, Bayreuth, Deutschland
tel: ++49 921 553745; fax: ++49 921 553769






From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Wed, 13 Nov 1996 10:40:58 -0600
Subject: High Resolution networked Printers

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Message-Id: {199611131644.KAA18820-at-watson.bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
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Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Whenever someone asks about High Resolution networked Printers, the
response is almost always "Go Dye-sub." In the EM business, especially in a
service situation, we may crank out 80 to 100 or more prints a day. $4 to $6
per print just ain't going to work. Fortunately other technologies (laser
and ink jet) seem to be making head roads into the problem. EM is monochrome
which helps considerably; and I would guess that a fair amount of light
microscopy, which we normally think of as color, could be usefully rendered
in shades of gray. What is needed is a printing technology that gives us
high resolution.....both spacial AND contrast......quickly and inexpensively
(pennies, not $'s per page).



Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: Giles John E Jr :      giles_john_e_jr-at-space.honeywell.com
Date: 13 Nov 1996 12:21:47 -0500
Subject: Kevex Printers

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Message-Id: {199611131711.LAA01738-at-Sparc5.Microscopy.Com}

Hello All,

We currently have a Kevex Delta V EDS system with an old Okidata 192 printer.
I was wondering if anyone had any experience with changing printers to
something more modern (laser) ?

We have Kermit, so we could load drivers into Kevex system if we knew
what/where to load them.

Any info would be greatly appreciated.

John Giles
Senior Engineer
Honeywell Space Systems
jegiles-at-space.honeywell.com




From: S Senthil Nathan :      sen-at-isu.iisc.ernet.in
Date: Thu, 14 Nov 1996 00:07:31 +0500 (GMT+0500)
Subject: reference

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Hi,
I am looking for secondary electron emission co-effecient for yttrium
barium copper oxide material and its temperature dependency. Could anyone
suggest me a reference.

thanx in advance

bye

sen


////
___|--00_____________________________________________________________________
C ^ S.Senthil Nathan
\ ~/ Dept. of Instrumentation, Indian Institute of Science,
{} {} Bangalore, India
e-mail:sen-at-isu.iisc.ernet.in
http://www.isu.iisc.ernet.in/~sen/
voice: +80 3092349
fax: 91 80 3345135










From: Carl Henderson :      chender-at-umich.edu
Date: Wed, 13 Nov 1996 14:40:37 -0500 (EST)
Subject: Re: Kevex Printers

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On 13 Nov 1996, Giles John E Jr wrote:

: We currently have a Kevex Delta V EDS system with an old Okidata 192 printer.
: I was wondering if anyone had any experience with changing printers to
: something more modern (laser) ?

I updated an old Microline 92 printer to a 9-pin dot matrix Okidata 320
about a year ago. The printer needs a serial port expansion card and
Microline (ML) emulation. The emulation is needed to handle the graphics
sent by the Kevex software when printing spectra. I don't know if there
are laser printers that support such emulation--maybe Okidata makes one.

Carl
Carl Henderson
Electron Microbeam Analysis Laboratory
University of Michigan
2005 C.C. Little Bldg.
Ann Arbor, MI 48109-1063 USA
--------------------------------
(313) 936-1550 (voice)
(313) 763-4690 (FAX)
chender-at-umich.edu (e-mail)
--------------------------------





From: Peter Miraglia :      pqmiragl-at-eos.ncsu.edu
Date: Wed, 13 Nov 1996 15:28:58 -0500
Subject: Backscattered Electrons - Use in imaging

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Message-ID: {328A2F8A.5ED4-at-eos.ncsu.edu}

Dear Staff:

I am gathering data for a research paper on the use of Backscattered
Electrons in imaging. I am not limiting my search to any one field. Thus
far, I have found some interesting items from the IEEE Society papers, a
physics journal, and the acoustics area. Please let me know of any new
developments in the use of backscattered electrons.

Since I am wrapping up my data gathering soon, (11-18-96), please let me
know soon. My email address is nrjackso-at-eos.ncsu.edu

thanks.

Nicole




From: Heike Buecking :      heibueck-at-uft.uni-bremen.de
Date: Thu, 14 Nov 1996 00:07:15 +0100
Subject: cryo-substitution

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Message-Id: {1.5.4.32.19961113230715.0069d17c-at-alf.zfn.uni-bremen.de}
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Hello everyone,

our institute want to buy a new cryo-substitution unit, especially to
dehydrate plant material after cryofixation. Which companies offered this
technique and what are your experiences. At the moment we have only contact
to Leica and Bal-tec.=20
Thanks

Heike Buecking
Dr. Heike Buecking
Universit=E4t Bremen, UFT
Physiologische Pflanzenanatomie
Leobener Str.
28359 Bremen
Tel: +49-0421-218-2954
-7283
Fax: +49-0421-218-3737





From: Analytical Imaging Facility :      aif-at-telico.bioc.aecom.yu.edu
Date: Wed, 13 Nov 1996 15:26:10 -0500 (EST)
Subject: Cy2?

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Has anybody tried Cy2 with the BioRad MRC 600 with the Kr/Ar laser?
According to the spectra on the mailing we just received from Jackson
Labs it should work fine and, potentially (depending on whether it is as
glaringly bright as we find Cy3 to be), less spillover into the red. But
has anybody any experience w/ it?

--------------------------------------------
email sent from an account of the Analytical Imaging Facility
The Albert Einstein College of Medicine of Yeshiva University
1300 Morris Park Ave. Bronx, NY 10461
(718) 430-2890 FAX: (718) 430-8996
--------------------------------------------






From: wcornell-at-centum.utulsa.edu (Winton Cornell)
Date: Wed, 13 Nov 1996 16:33:33 -0600
Subject: more on EDS spectra

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Microscopists:

Another EDS question - while we're on the subject these days:

I operate the EDS on our Camebax microprobe through/with the/a PCA II EDS
card (The Nucleus, Inc.). Although I routinely use/process/label spectra
with the handy-dandy (qualitative) functions of associate software, I am
now interested in export of the data to other off-line programs. Files are
saveable as comma-delimited ASCII files for just such processing.

My question then - is anyone out there using ASCII files from the PCA II
(or I or III, for that matter) for off-line processing? If so, what do you
use?.... user-programmed software?....other commercial EDS
software?....other spectral processing software?

Looking forward to your feedback.


Dr. Winton Cornell
Senior Research Associate
Department of Geosciences
The University of Tulsa
600 South College
Tulsa, OK 74104-3189

phone: 918-631-3248
fax: 918-631-2091
e-mail: wcornell-at-centum.utulsa.edu






From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Wed, 13 Nov 1996 17:23:24 -0700
Subject: TEM Price - Summary

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I received 14 responses to my question about a price increase for Kodak
4489 TEM film. Thank you, all.

There were replies from several EM supply houses who sell the Kodak
product. Apparently, not all sell the 250 sheet Multipack. At least one,
Energy Beam Sciences, sells AGFA SCIENTIA 23D56 film for TEM.

Other places mentioned are:

Laube Photo
Akron, OH
330-535-3379

Mike's Camera
Boulder, CO
303-443-1715
guide.boulder.net/Mikes/

National Graphic Supply
Albany, NY
800-223-7130
www.natgraphicsupply.com

Penn Camera
Washington, D.C. area
800-347-5770
www.penncamera.com

Prices quoted are about $60/box (100 sheets) and $110-120 for the Multipack
(250 sheets). It's time for me to switch to the Multipack!

All information received was for suppliers in the US and in US dollars.

Hope this helps.

John
chandler-at-lamar.ColoState.EDU






From: dwaters-at-api.com (Dean Waters)
Date: Wed, 13 Nov 1996 11:10:16 -0800
Subject: Job Opportunity

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******************************************************************************
Applied Precision, Inc. is seeking an Eastern Regional Sales Representative
for DeltaVision, our line of deconvolution microscope systems and related
products.
******************************************************************************

This is an opportunity to make a significant contribution to our rapidly
growing company and product line. To be considered, you must have at least 3
years highly successful experience in technical sales of confocal or similar
microscopy products.

Credentials should include: education in biology or related field, knowledge
of digital imaging, knowledge of Silicon Graphics computer system (or
similar skills), ability to travel up to 50% as needed.

For immediate consideration, send resume and salary requirements to:
Applied Precision, Inc.
Attn: DV
1040 12th Avenue NW
Issaquah, WA 98027

For more information, visit our booth at the Society for Neuroscience
meeting in Washington, D.C.

________________________________________________________________________
Dean Waters
DeltaVision Systems
Applied Precision, Inc.
1040 12th Avenue NW
Issaquah, WA 98027-8929

dwaters-at-api.com
(206) 557-1000 x4408
(206) 557-1055 FAX





From: shaf :      mshaf-at-darkwing.uoregon.edu
Date: Wed, 13 Nov 1996 11:28:11 -0800
Subject: Re: High Resolution networked Printers

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Message-Id: {3.0b36.32.19961113112809.006c7948-at-darkwing.uoregon.edu}
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At 10:40 AM 11/13/96 -0600, Joiner wrote:

} ... , we may crank out 80 to 100 or more prints a day. $4 to $6
} per print just ain't going to work.

The least expensive way to go would be a 600dpi Laser which is fast and
suits your costs per page. The problem we've seen with lasers is that if
you print a lot of images then you will notice problems with the toner
cartridge about half way thru its normal lifetime (we take it out and put
into a similar printer dedicated to line drawings or text).
A monochrome ribbon for a dye sub will be considerable less expensive
than dedicating it to color, but if you ever did want to use it for color
then it is a real hassle just to change it over for a job.

Lastly ... you might want to look into a Ag salt laser (e.g., Harris
PhotoPro), for about a dollar/page you can have 256 "real" grays at 256dpi
on glossy paper which may have been improved since we bought ours. The
problem with this printer is it can not be configured as a network printer,
but you can certainly install it onto a peer-to-peer, or networked computer.

cheers, shaf



{\/} /\ {\/} /\ {\/} /\ {\/} cognito, ergo zZOooOM {\/} /\ {\/} /\ {\/} /\ {\/}
Michael Shaffer, R.A. - University of Oregon Electron Probe Facility
mshaf-at-oregon.uoregon.edu -or- mshaf-at-darkwing.uoregon.edu
http://darkwing.uoregon.edu/~mshaf/epmahome/





From: RPETER041-at-aol.com
Date: Wed, 13 Nov 1996 22:50:53 -0500
Subject: Re: ultramicrotomy of paper

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One suggestion not mentioned in earlier replies from excellent sources to
this inquiry is to use the sample block in the SEM and not just the section.
Of course, beam penetration must be kept in mind when doing work such as BE
imaging.

This may be obvious but it does make sample prep somewhat easier...

R. Peterson




From: Christophe Roos :      ROOS-at-operoni.helsinki.fi
Date: Thu, 14 Nov 1996 07:32:03 EET DST
Subject: Re: High Res. Print - Dye sub etc

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} Whenever someone asks about High Resolution networked Printers, the
} response is almost always "Go Dye-sub." In the EM business, especially in a
} service situation, we may crank out 80 to 100 or more prints a day. $4 to $6
} per print just ain't going to work.

Right, why then not go for a dual solution: dye-sublimation for
photorealistic pictures (material for publications) at 5$ per one
print and then thermal wax for drafts (300dpi, good color
saturation, 5$ per 100 prints)? Color lasers have not convinced me
yet (pale rendition) but are improving.

A good thermal wax printer is about 60% of the price of a good
dye-sublimation printer. Color lasers are coming down in price.

ChR

_________________________________________________________________________
Christophe Roos Dr.Sc., doc. | Institute of Biotechnology
| & Dept. of Biosciences
Phone: +358 9 7085 9367 | Division of Genetics
Fax: +358 9 7085 9366 | P.O.Box 56, Viksbagen 9
E-mail: Christophe.Roos-at-Helsinki.FI | FIN-00014 Univ. of Helsinki
X-400: /G=Christophe/S=Roos/O=Helsinki/ADMD=fumail/C=Fi Finland
{A HREF="http://www.helsinki.fi/~roos/index.html"} WWW Home Page: Roos {/A}
-------------------------------------------------------------------------




From: Paul Vanderlinden :      orion-at-infoboard.be
Date: Thu, 14 Nov 1996 10:34:37 +0100 (MET)
Subject: Orion 4 grabbing system

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Dear Mr Deking,

On october 4th, I send you some information about our high resolution
grabbing system for SEMs.

Could you please let me know you comments and interrest about it ?

Thanks in advance.


Best regards,



Paul Vanderlinden.
Sales Manager.

=========================================================================
See our web site: http://www.microscopy-uk.org.uk

To contact us:

E.L.I. sprl

Technical support:
Jean-Louis Leclef: Phone: +32 67 21 25 07
Fax : +32 67 22 09 53
Email: jleclef-at-hypercon.com
Sales support:
Paul Vanderlinden: Phone: +32 2 215 20 02
Fax : +32 2 726 08 65
Email: orion-at-infoboard.be
=========================================================================







From: Mr L Scott :      SCOTT-at-getafix.utr.ac.za
Date: Thu, 14 Nov 1996 11:59:27 GMT+02.00
Subject: proposal

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Hi
We are thinking of establishing an electron microscope unit
(mainly for biological work) from scratch. That means that we have
to put a proposal together for funding of equipment and staff.

Are there any suggestions on how to go about?

L. Scott
Univ. Transkei
South Africa




From: timon.fliervoet-at-uni-bayreuth.de (Timon Fliervoet)
Date: Thu, 14 Nov 1996 14:10:55 +0100
Subject: TEM: EDS software-3

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Dear all,

To follow up on my initial question on offline EDS software. I had several
replies. The one from Henrik Kaker pointed towards the microscopy ftp
sites. I downloaded some of the programs and found that the programs
capable of dealing with thin films cannot deal with my oxygen measurements
in TEM. Does anybody have another suggestions. Is the TFA (Thin filam
Analysis) program, written by Martin J. carr and Alton D. Romig being
updated??

Any help and suggestions are greatly appreciated.

Cheers, Timon

---------------------
Timon Fliervoet
Bayerisches Geoinstitut, Universitat Bayreuth
D-95440, Bayreuth, Deutschland
tel: ++49 921 553745; fax: ++49 921 553769






From: G.Fourlaris :      METGF-at-LUCS-MAC.NOVELL.LEEDS.AC.UK
Date: Thu, 14 Nov 1996 12:33:25 GMT0BST
Subject: Problems with Electropolishing Al -7000 series alloys

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Fellow Microscopists
One of our Research students, is carrying out a Ph.D project on Al based
alloys (Spay cast 7xxx series) , (10-11% Zn, 1-1.5% Mg, 1.5-2 % Cu, 0.3
% Zr, Al balance). As part of her project she is required to carry out an
extensive TEM characterisation study of these alloys.
However, she is encountering significant problems with rapid oxidation
of the TEM samples, following preparation by a conventional twin jetting
technique (involving the use of a Struers Tenupol -3 model using an
electrolyte solution of 30% Nitric acid in methanol at -40oC).
Following preparation by twin jetting we have tried removing the oxide
films by ion beam thinning on a Gatan ion beam thinner, at a milling
angle of 10o (minimum angle permitted ) and at a voltage of 4KV and at a
current of 3mA.
Following this dual approach, some times Oxide free Aluminium foils are
prepared but some times the oxide formation persists. However, it
appears that this effect is not related to the milling time, since different
milling times were involved.
I would deeply appreciate your advise on this persistent problem, by
either their replying to the mailing list or to her directly at:
meteo-at-leeds.ac.uk

Many thanks in anticipation.

George





From: Joergen Bilde-Soerensen 5802 :      j.bilde-at-risoe.dk
Date: Thu, 14 Nov 1996 14:10:12 +0100
Subject: Re: Variable Pressure SEM

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Mike Warfield wrote:
} I am looking for reference material about application work being
} done using the new variable pressure sems. Can you point me to any
} specific references of libraries that might have such materials.

Dear Mike,

You can find a bibliography of environmental scanning electron
microscopy in:
G. D. Danilatos, Microscopy Research and Technique, vol. 25 (1993)
page 529-534.

There is also a bibliography in ElectroScan's homepages on the
address:
http://www.electroscan.com/bibliog.html

One major problem in the application of variable pressure SEMs is
that the spatial resolution for X-ray spectrometry deteriorates with
increasing pressure because the primary electrons are scattered on
the gas and therefore ends up far from the electron beam target
point. Concerning ways to overcome this procblem we have recently
published two conference papers:

J. B. Bilde-Sorensen and C. C.Appel, Proc. 48th Annual Meeting of the
Scandinavian Society for Electron Microscopy, Aarhus, 2-5 June 1996.
p. 4-5
J. B. Bilde-Sorensen and C. C. Appel, Proc. 11th European Congress on
Microscopy EUREM'96, Dublin, 26-30 August 1996. Session T6.

Best wishes,
Jorgen.
-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
J. B. Bilde-Soerensen
Materials Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 35 11 73




From: Woody.N.White-at-mcdermott.com
Date: 11/13/96 11:21 AM
Subject: Kevex Printers

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Message-Id: {199611141300.IAA27034-at-ns1.axs2000.net}
To: MICROSCOPY BB {Microscopy-at-Sparc5.Microscopy.Com}
microscopy-at-sparc5.microscopy.com
P1-Message-Id: US*MCI*MCDERMOTT;c\650\961114072055c
Original-Encoded-Information-Types: IA5-Text
X400-Trace: US*MCI*MCDERMOTT; arrival 961114080600-0600 deferred 961114080600-0600 action Relayed
Message-Id: {M1893689.004.v3qt3.2282.961114132055Z.CC-MAIL*/O=650/PRMD=MCDERMOTT/ADMD=MCI/C=US/-at-MHS}
P1-Content-Type: P2

Hello John,

Good Luck!!! I have tried to replace my Tek 4696 injet on my
delta-like Kevex 8005 to no avail. "Drivers" for the DEC system (in
my case) are unique programs. I could never locate either a
substitute printer or a printer program for a more modern printer. If
I recall correctly, the Oki command structure is incompatible with
newer printers and would then present similar problems. I had to add
a video printer to replace the Tek. Still am funning the 192, but use
it only for text. If unformatted ascii is all you print, your problem
may not bee insurmountable, but I needed to go to the "graphics" mode.

The company who now owns the rights to the vintage DEC system (not
DEC) offered to develop someting, buyt couldn't pin down a price
except to say that it would be $10K, maybe $20K. Kinda pricey driver,
huh? Maybe this is an opportunity for some enterprising (old)
programmer. :-)

If you find more, helpful info, please forward.

Woody White, Electron Microscopist
Babcock & Wilcox Research


______________________________ Reply Separator _________________________________


Hello All,

We currently have a Kevex Delta V EDS system with an old Okidata 192 printer.
I was wondering if anyone had any experience with changing printers to
something more modern (laser) ?

We have Kermit, so we could load drivers into Kevex system if we knew
what/where to load them.

Any info would be greatly appreciated.

John Giles
Senior Engineer
Honeywell Space Systems
jegiles-at-space.honeywell.com




From: rnbalduc-at-alpha.arcride.edu.ar
Date: Thu, 14 Nov 1996 14:17:34 -1356
Subject: Searchin used SEM

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Dear Microscopysts

We are searching an used SEM for received it in donation .The SEM will be used for Research and Education in our School.
Please, we need know the model, year of fabrication, possibility of work,and other information available.
WE PAY ALL THE COSTS OF SHIPMENT.
Please, contact me for any questions.

E-mail : RNBALDUC-at-arcide.edu.ar

Address:

Fernando Balducci
Laboratory of Electron Microscopy
School of Bioengineering.
National University of Entre Rios.
C.C 57 Suc 3
Parana - Entre Rios
Argentina.





From: Chao-ying Ni; Office 312 SPL; Phone 1013 :      ni-at-me.udel.edu
Date: Thu, 14 Nov 1996 10:02:26 -0500 (EST)
Subject: X-TEM thin films: beam sensitive substrate

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Dear all,

I am working on the X-TEM of multilayer thin films on a soda-lime
substrate. What was unexpected was that when the beam (~120KeV) hit the
substrate, it evaporated (melted?) and the films collapsed. Can anybody
give me any suggestions on dealing with such specimens?

Your help are greatly appreciated.


Chao-Ying Ni
Institute of Energy Conversion
University of Delaware
Newark, DE 19716








From: Paul Vanderlinden :      orion-at-infoboard.be
Date: Thu, 14 Nov 1996 17:17:37 +0100 (MET)
Subject: Orion 4 - apology

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Dear Microscopists,

I am really sorry.
Due to my hurry, I have sent to all of you a personnal message without any
general interest.
I'll try and do my best in the future to be more careful :-( :-( :-(


Best regards,



Paul Vanderlinden.
Sales Manager.

=========================================================================
See our web site: http://www.microscopy-uk.org.uk

To contact us:

E.L.I. sprl

Technical support:
Jean-Louis Leclef: Phone: +32 67 21 25 07
Fax : +32 67 22 09 53
Email: jleclef-at-hypercon.com
Sales support:
Paul Vanderlinden: Phone: +32 2 215 20 02
Fax : +32 2 726 08 65
Email: orion-at-infoboard.be
=========================================================================







From: Richard Thrift :      Richard_Thrift-at-depotech.com
Date: Thu, 14 Nov 1996 11:52:33 -0800
Subject: Repeat, does DiI flip-flop across membrane bilayers?

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Message-Id: {s28b07a4.037-at-depotech.com}
X-Mailer: Novell GroupWise 4.1

I'll try one last time; got no response the first time.

My main question: Can you tell me whether DiI (and related
dyes) flip-flop across membranes to label both sides of a
bilayer (& if so does it require the presence of ATP)? I know
the usual answer is "Check the Molecular Probes catalog"
but the catalog says "there are discrepancies in the
literature".

Also, I've heard DiI labels all internal cell membranes; is this
true? If so does it diffuse through cytoplasm, is it carried by
an exchange protein, or is it really constrained to stay in the
plane of the outermost membrane? Can it jump from one
membrane to another that is very closely apposed but not
continuous? Can you suggest any good references on
properties of these dyes?

Pardon my ignorance; any suggestions would be greatly
appreciated!
THANKS very much !
Richard Thrift
Depotech Corp.
Richard_Thrift-at-Depotech.com
(619) 625-2424







From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 14 Nov 1996 09:30:35 -0600
Subject: Re: High Resolution networked Printers

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Message-Id: {199611141534.JAA10147-at-watson.bcm.tmc.edu}
X-Sender: joiner-at-bcm.tmc.edu
X-Mailer: Windows Eudora Version 2.1.1
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

At 08:22 AM 11/14/96 +0001, you wrote:
} The obvious solution, though not necessarily practical financially
} speaking would be to have a reasonable laser printer for day to day
} archiving type of work and a good dye sublimation printer to
} be used only for those situations where publication quality is
} required. The latter printer could be located in a institutional
} central area and jobs sent remotely, while the laser writer would
} be located locally. One can justify the dye sub printer costwise,
} by comparing to the upkeep of a print darkroom. In addition, AFM
} images and the like, for publication really cannot be output any
} other way, not to mention the "fancy", full color transparencies
} that can be produced!
}
*****************
No, that won't work. "Day to Day" is where we need the quality. We produce a
large number of E.M. images upon which we base medical diagnoses. These
prints have to have and convey the maximum amount of information captured in
the original image. The publication images are best produced in the darkroom.





From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Thu, 14 Nov 1996 08:55:15 -500
Subject: Win NT and Image capture

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OS wars:

DOS/Win 3.x - Its dead, Bill-I-am-master-of-the-universe-Gates
has killed it. (Besides it won't handle over 64Mb of RAM any way -
Don't believe me? Go ahead and try it)

OS/2 - stabile, once you get it loaded, but its destined to die an
undeserved death since no one is writing software specficially for
this OS.

UNIX/AIX/LINUX/Etc. - Give us a break.

Win 95 - A toy for the masses, and not really 32-bit.

Windows NT - ? Is this what we're left with? Its far more stabile
than Win 95, and is designed to handle networking issue much better.
It'll be around for awhile yet. But inherent in its stability is its
limitations on software control of hardware (that's why gamers don't
like it), but due to the massive quantities of data, data
transmission rates, and our hopes of 'live' image capture, the
typical solution has been to by pass the OS and have the software
directly handle the hardware (i.e. capture card) and memory
transfers in order to save the time wasted in OS overhead. What
does this mean interms of Windows NT and PCI capture systems?

I am looking for any KNOWN information on hardware interface
problems with Windows NT and 'image' capture hardware. Alternately,
or additionally, we would (I know I'm not alone) love to hear from
digital imaging vendors to find out where the OS wars are heading to
for microscopy needs.

Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu




From: timon.fliervoet-at-uni-bayreuth.de (Timon Fliervoet)
Date: Thu, 14 Nov 1996 16:56:02 +0100
Subject: TEM: EDS software-3

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Dear all,

To follow up on my initial question on offline EDS software. I had several
replies. The one from Henrik Kaker pointed towards the microscopy ftp
sites. I downloaded some of the programs and found that the programs
capable of dealing with thin films cannot deal with my oxygen measurements
in TEM. Does anybody have another suggestions. Is the TFA (Thin filam
Analysis) program, written by Martin J. carr and Alton D. Romig being
updated??

Any help and suggestions are greatly appreciated.

Cheers, Timon

---------------------
Timon Fliervoet
Bayerisches Geoinstitut, Universitat Bayreuth
D-95440, Bayreuth, Deutschland
tel: ++49 921 553745; fax: ++49 921 553769






From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Thu, 14 Nov 1996 08:44:07 -0600
Subject: Re: Fume Hood - suggestions

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} Our lab is in the process of buying a fume hood. It will be used primarilly
} for protection against EM and LM processing chemicals (fixatives, resins,
} solvents, etc.).
} My question is basically: what characteristics are recommended? (do we need a
} filter on the exhaust system? If so, what type of filter?) I would appreciate
} any suggestions.
} Thanks in advance.
}
} Adriana
}
A filter would be nice. Also, it's easier to build in than
retro-fit, so even if you don't want one now, it's better to build for it.
You'd need filters for organics and osmium.
Face velocity: fume hoods here are typically 100-120 cubic
feet/minute with the door open 12-18" (variations by state and
institution).
Watch where the exhaust is. I've been places where the fume hood
exhaust mixed into the building air, or is exhausted at ground level next
to busy sidewalks. It should be on the roof, and any scubbers ought to be
there also.
Also: don't put a storage cabinet under the fume hood! This is a
common practice, and is part of the cabinet. It only incourages people to
store acids, bases, and volatile organics together. Plus it makes working
at the hood, doing perfusions, etc. very awkward. Put a foot well under the
fume hood.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel *all mail:*
Microscopy
University of Illinois Station A
Rm 74 Bevier Hall PO Box 5037
905 S. Goodwin Ave. Champaign, IL 61825-5037 USA
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu
(217) 355-1143 home

*********** looking for a job again ***********






From: Laurence Tetley :      gbza40-at-udcf.gla.ac.uk
Date: Thu, 14 Nov 1996 15:40:15 GMT
Subject: Re: Fume Hood - suggestions

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Adriana

Not quite answering your question but have you thought of including a fume
bench in your installation ? We use this as frequently as the small fume
hood we had originally especially for the less hazardous processing
chemicals such as solvents and resins. Using an open access downdraught
fume bench is far easier when attempting to manipulate small specimens -
sometimes using a dissection microscope. We still use osmium in the hood
though ! Check your hospital pathology labs - they go in for fume benches
due to rigorous handling regulations which now apply to formaldehyde.

Good liuck

Laurence Tetley





At 09:20 14/11/96 EDT, you wrote:
}
} Our lab is in the process of buying a fume hood. It will be used primarilly
for protection against EM and LM processing chemicals (fixatives, resins,
solvents, etc.).
} My question is basically: what characteristics are recommended? (do we need
a filter on the exhaust system? If so, what type of filter?) I would
appreciate any suggestions.
} Thanks in advance.
}
} Adriana
}
} Adriana P. M. Rodriguez
} Biologia Celular, CENA/USP
} Av. Centenario 303, C.Postal 96
} 13400-970, Piracicaba, SP, BRAZIL
} fax 55-19-429 4610 - voice 55-19-429 4694
} e-mail {adriana-at-aguia.cena.usp.br}
}
}
}
Dr Laurence Tetley
IBLS EM Centre
Joseph Black Building
University of Glasgow
Glasgow G12 8QQ

email gbza40-at-udcf.gla.ac.uk
tel. 0141 330 4431
fax 0141 307 8016





From: jmkrupp-at-cats.ucsc.edu (Jon Krupp)
Date: Thu, 14 Nov 1996 15:07:14 -0800
Subject: Looking for a microtome

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I think we would like to upgrade our ultramicrotome resources. We have an
MT-2B and an MT-2. We do mostly simple thin sectioning with a fair number
of students and novice users.

I am looking for expert (!) advice on the following questions:

1. If we can get the funds together, would you like to volunteer your
opinions on the products currently available? (Where is Consumer Reports
when you really need them?)

2. If we can't get enough together for a new instrument, what's the
used microtome market like these days?

3. Should we be quiet and be happy with our MT-2B?

Jonathan Krupp
Microscopy and Imaging Lab
University of California
Santa Cruz, CA 95064
(408) 459-2477
FAX (408) 429-0146
jmkrupp-at-cats.ucsc.edu






From: akracher-at-iastate.edu (Alfred Kracher)
Date: Thu, 14 Nov 1996 12:08:39 -0600
Subject: Multiple printers

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} ...why then not go for a dual solution: dye-sublimation for
} photorealistic pictures (material for publications) at 5$ per one
} print and then thermal wax for drafts (300dpi, good color
} saturation, 5$ per 100 prints)?

In our (somewhat special) situation we actually have a triple solution: the
PC interfaced to the machine has a conventional laser printer, a color
inkjet, and it is also networked to campus services. Color inkjets are now
capable of producing "working quality" on plain paper, and relatively good
(what I would call "poster quality") on coated paper--at $2 or so a shot.
The final "publication quality" step is achieved by sending pictures (after
careful preview, of course) to services on campus, like a truly
professional color printer or slide camera. For the number of times we
really need dye sub quality it is cheaper to pay them $6 a shot than to buy
our own printer. Although these options are not available to all EM
facilities, those in large research universities should carefully explore
the services available to them.

Alfred

-----------------------------------------
Alfred Kracher
akracher-at-iastate.edu
http://www.public.iastate.edu/~akracher
-----------------------------------------






From: Warren Straszheim :      wes-at-ameslab.gov
Date: Thu, 14 Nov 1996 08:15:51 -0600
Subject: Re: Kevex Printers

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The nature of the PDP-11 and the RT-11/TSX operating system is somewhat
similar to the PC and DOS. A printer driver needs to be written for every
application. There is no general purpose driver that can be loaded to
support a new printer like can be done under Windows or Mac-OS. The driver
that is part of the RT-11 operating system simply manages the transmission
of bytes from the CPU to the serial port. Therefore, you would need a
version of the program that already has laser printer support built in.

I seem to recall that Kevex had support for some laser printer, but my
information is buried quite deep. You may want to give them a call.

It may be that you can find a laser printer that uses the same control code
set that the Okidata printer used. I don't recall if it was similar to the
Epson code set or not. Even if it does emulation, you may not be happy with
the graphic rendition. That is, it may be sharper and blacker but still very
sparse and Spartan graphics.

The HP 4 printers support multiple personalities and can handle HPGL plotter
code, once the correct escape sequences are used to enable that mode. That
might be one solution for you and might allow you to use the HPPLOT program
that Kevex supplied. I might be able to help you with the details of
switching modes.

If you are interested in printing images, you might be better off saving the
image to disk and shipping it to a PC or Mac for printing as a TIF file. I
have a routine I wrote to do that. I will see about getting it onto the MSA
file server.

At 12:21 PM 11/13/96 -0500, you wrote:
} Hello All,
}
} We currently have a Kevex Delta V EDS system with an old Okidata 192 printer.
} I was wondering if anyone had any experience with changing printers to
} something more modern (laser) ?
}
} We have Kermit, so we could load drivers into Kevex system if we knew
} what/where to load them.
}
} Any info would be greatly appreciated.
}
} John Giles
} Senior Engineer
} Honeywell Space Systems
} jegiles-at-space.honeywell.com
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Dr. Steve Zullo :      zullo-at-helix.nih.gov
Date: Thu, 14 Nov 1996 14:12:26 -0500
Subject: FW: Notification: Inbound Mail Failure - Address not found

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Hi microscopy list, sorry but I am forwarding this which was forwarded to me
to alert the list-manager to remove my old address from the list
Thanks,
Steve Zullo


} X-PH: V3.18-at-itchy.dcrt.nih.gov
} From: "Waldman, Ivan N." {WALDMANI-at-dirpc.nimh.nih.gov}
} To: "Zullo, Steven {sz5m} " {sz5m-at-nih.gov}
} Subject: FW: Notification: Inbound Mail Failure - Address not found
} Date: Thu, 14 Nov 1996 12:37:36 -0500
} Encoding: 27 TEXT
}
} Steve,
} You are getting a large number of e-mail messages still being sent to your
} old address --i.e. here at SEH. Can you please notify everyone of your new
} address as this is causing problems.
} Thanks.
} Ivan Waldman
}
} ----------
} From: System Administrator[SMTP:postmaster-at-imc1.nih.gov]
} Sent: Wednesday, November 13, 1996 11:14PM
} To: NIH Hub Administrator
} Subject: Notification: Inbound Mail Failure - Address not found
}
} A mail message was not sent because the following address(es) could not be
} found:
}
} {zullos-at-dirpc.nimh.nih.gov} zullos-at-dirpc.nimh.nih.gov
}
} The message that caused this notification was:
}
} To: {microscopy-at-Sparc5.Microscopy.Com}
} From: {Microscopy-request-at-Sparc5.Microscopy.Com}
} Subject: Re: High Resolution networked Printers
}
}
}
}
}





From: ROOS-at-operoni.helsinki.fi at -SMTPlink
Date: 11/13/96 9:32 PM
Subject: Re: High Res. Print - Dye sub etc

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I'm not sure what setup you are using for dye-sublimation printing, but our
Kodak 8650PS (about $8k after rebate) will print 8"x10" gray scale prints for
about $1.75 (U.S.) per print. Even the three color prints are only about $2.20
per print. Are you figuring in depreciation on the $4-$6 per print? Also, you
can easily put multiple prints on one page, reducing the cost further. As has
already been mentioned in this thread, the Codonics will do this automatically
and easily (and costs the same per print since the insides are the same as the
Kodak and takes the same supplies), though that printer costs a bit more.

This cost is still higher than laser or thermal wax, but it is not as bad as
originally presented.

I have no business interests in either Kodak or Codonics, but have been happy
with our Kodak printer.

Cheers,

John Vetrano
js_vetrano-at-pnl.gov
_______________________________________________________________________________

} Whenever someone asks about High Resolution networked Printers, the
} response is almost always "Go Dye-sub." In the EM business, especially in a
} service situation, we may crank out 80 to 100 or more prints a day. $4 to
$6
} per print just ain't going to work.

Right, why then not go for a dual solution: dye-sublimation for
photorealistic pictures (material for publications) at 5$ per one
print and then thermal wax for drafts (300dpi, good color
saturation, 5$ per 100 prints)? Color lasers have not convinced me
yet (pale rendition) but are improving.

A good thermal wax printer is about 60% of the price of a good
dye-sublimation printer. Color lasers are coming down in price.

ChR

_________________________________________________________________________
Christophe Roos Dr.Sc., doc. | Institute of Biotechnology
| & Dept. of Biosciences
Phone: +358 9 7085 9367 | Division of Genetics
Fax: +358 9 7085 9366 | P.O.Box 56, Viksbagen 9
E-mail: Christophe.Roos-at-Helsinki.FI | FIN-00014 Univ. of Helsinki
X-400: /G=Christophe/S=Roos/O=Helsinki/ADMD=fumail/C=Fi Finland
{A HREF="http://www.helsinki.fi/~roos/index.html"} WWW Home Page: Roos {/A}
-------------------------------------------------------------------------




From: Joiner Cartwright, Jr., Ph.D. :      joiner-at-bcm.tmc.edu
Date: Thu, 14 Nov 1996 13:34:51 -0600
Subject: Re: High Resolution networked Printers

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At 12:16 PM 11/14/96 +0001, you wrote:
} Well, if you really need the highest (to me, that means publication
} quality)quality output on a day-to-day basis, then your organization
} should recognize this and support the costs incurred. One question,
} though, if this is really the case, then why would you need a print
} darkroom at all?
}
*********************
I am trying to GET OUT OF THE DARKROOM! The world has decided that health
care should be cheap, and the competition between providers has mandated
that results should be delivered fast. My department will pay for the
machine, but it is the HMO's etc. that will (Ho-ho-ho!) or should, pay for
my services. If you have been reading the news in the past year or so, you
will know that THEY want our services done for free! By digital capture of
images, archiving on CD ROM, and immediate print out of the images I am
hoping to shave maybe an entire day off of the turn around time for a case
as well as some of the cost. Developing, washing, drying, printing
negatives; then washing and drying the prints takes a lot more time. And
when you add up the cost of the materials, chemicals, and most importantly,
the technician's wages, you have spent serious money. Then when you try to
get the print sets back from the various pathologists and clinicians in
order to perform the Quality Assurance duties that are now mandated, and
they have been misplaced, or "Dr. So-In-So is out of town, but he'll be sure
to return them to you as soon as he gets back.......next month", the idea
that your computer can print out a new set rather quickly, becomes rather
attractive. In short, digital management and data basing of images is
becoming more efficient than the old darkroom technology.


Joiner Cartwright, Jr., Ph.D.

Director, Electron Microscopy tel.: (713)798-4658
Department of Pathology, Rm.286-A FAX: (713)798-3945
Baylor College of Medicine Internet: joiner-at-bcm.tmc.edu
One Baylor Plaza Compuserve: 71555,1206
Houston, Texas 77030 U.S.A.






From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Fri, 15 Nov 1996 13:47:02 +1100 (EST)
Subject: Re: High Resolution networked Printers

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} Whenever someone asks about High Resolution networked Printers, the
} response is almost always "Go Dye-sub." In the EM business, especially in a
} service situation, we may crank out 80 to 100 or more prints a day. $4 to $6
} per print just ain't going to work.

You seldom need to print each negative on a whole letter size page. We
usually put from 2 to 6 on a dye sub page. Thats quite economic. And where
you REALLY need photo quality in submitting to a journal, (1) Laser output
wont do (2) you have 4-6 on one page anyway.



Fortunately other technologies (laser
} and ink jet) seem to be making head roads into the problem.

They are fine for draft prints. So long as these show the image that is
needed, they are OK. Most of our work can be output on our laser.

EM is monochrome
} which helps considerably; and I would guess that a fair amount of light
} microscopy, which we normally think of as color, could be usefully rendered
} in shades of gray. What is needed is a printing technology that gives us
} high resolution.....both spacial AND contrast......quickly and inexpensively
} (pennies, not $'s per page).
}
} It sure would be nice, but I gave up holding my breath. What will be best
is Customers and Journals which accept digital images on disk or down the
net. We encourage customers to accept images on CD-ROM.



Mel Dickson





From: kszaruba-at-MMM.COM (Karen S. Zaruba)
Date: Thu, 14 Nov 1996 17:04:51 -0600
Subject: Image Analysis Equipment questions

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I am in the process of writing up a wish list of equipment to upgrade our
image analysis capabilities. Before I get to my questions, I should mention
that our lab examines primarily biological specimens & some polymers, where
good color reproduction of histological stains is needed. We have a Zeiss
(universal? 160mm) light microscope and a Wild dissecting scope, both
equipped with camera C-mount. We also do TEM and SEM and have occasion to
examine some macro samples on a copy stand. We may in future do some
fluorescent microscopy but this is not an immediate need. Also we have a
mix of PC's and Mac's but the major imaging work is done on PC's.

I am actually narrowing down my list of options for cameras, software, etc.
But there are still many unknowns. So, I would like to hear from anyone
with experience with the following instruments or suggestions as to which
models, etc. to choose (all opinions welcome!):

Cameras:

Optronics DEI750 vs.
Sony SODXC-970M vs.
Pixera Professional vs.
Kodak DCS 410 or 420 vs.
any other suggestions?

Technical question--if signal/noise ratio = 6(bits)+ 2 then how can Pixera
Professional be 24 bit and only 46 S/N?


Framegrabbers:

I know Matrox and Data Translation offer good products, but which models
will be complementary to the above cameras? Are any true RGB?


Computer:

I have given up on trying to make do with what I have. So if I am going to
order a new one, how does the following sound: Pentium with PCI bus, 40+
Meg RAM, 1+gig hard drive, [good graphics card ?? how to specify?], SCSI
port, 2 parallel ports, ZIP drive. What type of monitor is needed? And the
big question -- Windows 95 or NT??

Software:

BioScan Optimas vs. Media Cybernetics Image Pro Plus?


Misc:

Does anyone know of a good source for RG-6 75 ohm cable with quad shielding
that I can get custom made at certain lengths ( {50 feet) with F-connectors
and/or BNC's?


Thanks in advance for all suggestions and help!

Karen Zaruba

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
3M Company
3M Center 270-1S-01 Phone: 612-737-2971
St. Paul, MN 55144-1000 Fax: 736-1519

These opinions are my own and may not represent those of 3M.







From: A. Kent Christensen :      akc-at-umich.edu
Date: Thu, 14 Nov 1996 19:53:12 -0500 (EST)
Subject: Re: Win NT and Image capture

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Another combatant in the OS wars has been Macintosh (still a major player
in the digital/graphics realm), with OS-8 (Copland) being pursued in
gradual steps, but still a long way off. However, according to MacWeek
11.04.96, Apple is currently negociating with Be Inc. to buy BeOS, an
elegant, advanced, modern OS that already has preemptive multitasking,
symmetrical multiprocessing, multithreading, protected memory, and great
stability. BeOS is currently PowerPC native, and the plan would be to use
BeOS as the Macintosh operating system, with the OS-8 microkernel
(essentially ready) underneath it. Apple would continue to support OS-7.5
for the time being, and would plan to have the BeOS-OS8 hybrid out by next
summer. Although there would be a lot of problems to work out, it could
be quite an exciting system with impressive specifications. Many of the
major digital/graphics developers still have considerable Apple loyalty.
Apparently, Adobe is already working on a BeOS version of Photoshop.

A. Kent Christensen
University of Michigan
{akc-at-umich.edu}

------------------------------

On Thu, 14 Nov 1996, Richard E. Edelmann wrote:

} OS wars:
}
} DOS/Win 3.x - Its dead, Bill-I-am-master-of-the-universe-Gates
} has killed it. (Besides it won't handle over 64Mb of RAM any way -
} Don't believe me? Go ahead and try it)
}
} OS/2 - stabile, once you get it loaded, but its destined to die an
} undeserved death since no one is writing software specficially for
} this OS.
}
} UNIX/AIX/LINUX/Etc. - Give us a break.
}
} Win 95 - A toy for the masses, and not really 32-bit.
}
} Windows NT - ? Is this what we're left with? Its far more stabile
} than Win 95, and is designed to handle networking issue much better.
} It'll be around for awhile yet. But inherent in its stability is its
} limitations on software control of hardware (that's why gamers don't
} like it), but due to the massive quantities of data, data
} transmission rates, and our hopes of 'live' image capture, the
} typical solution has been to by pass the OS and have the software
} directly handle the hardware (i.e. capture card) and memory
} transfers in order to save the time wasted in OS overhead. What
} does this mean interms of Windows NT and PCI capture systems?
}
} I am looking for any KNOWN information on hardware interface
} problems with Windows NT and 'image' capture hardware. Alternately,
} or additionally, we would (I know I'm not alone) love to hear from
} digital imaging vendors to find out where the OS wars are heading to
} for microscopy needs.
}
} Richard E. Edelmann
} Electron Microscopy Facility Supervisor
} 352 Pearson Hall
} Miami University, Oxford, OH 45056
} Ph: 513-529-5712 Fax: 513-529-4243
} E-mail: edelmare-at-muohio.edu
}





From: Mary Mager :      mager-at-unixg.ubc.ca
Date: Thu, 14 Nov 1996 22:10:20 -0800
Subject: Re: Kevex Printers

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Dear John,
My experience with Kevex printers is that you can only get a new printer
from Kevex. Not only did they write all their own drivers for the graphics
screen dump, but they put their own EPROMS, labeled "Kevex", into the
printer. Kevex sold me a new Okidata Microline 320 when I complained about
the old printer, for US$600. Pretty good for a nine-pin dot matrix printer!
It does work better, though.
Good luck,
Mary

} Hello All,
}
} We currently have a Kevex Delta V EDS system with an old Okidata 192 printer.
} I was wondering if anyone had any experience with changing printers to
} something more modern (laser) ?
}
} We have Kermit, so we could load drivers into Kevex system if we knew
} what/where to load them.
}
} Any info would be greatly appreciated.
}
} John Giles
} Senior Engineer
} Honeywell Space Systems
} jegiles-at-space.honeywell.com
}
}
Mary Mager
Electron Microscopist
Metals and Materials Eng., UBC
6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel:604-822-5648, fax:604-822-3619
e-mail: mager-at-unixg.ubc.ca





From: Jim Darley :      p&s-at-ultra.net.au
Date: Fri, 15 Nov 1996 18:21:56 +1100
Subject: Re: X-TEM thin films: beam sensitive substrate

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At 10:02 14/11/96 -0500, you wrote:
}
} Dear all,
}
} I am working on the X-TEM of multilayer thin films on a soda-lime
} substrate. What was unexpected was that when the beam (~120KeV) hit the
} substrate, it evaporated (melted?) and the films collapsed. Can anybody
} give me any suggestions on dealing with such specimens?
}
} Your help are greatly appreciated.
}
}
} Chao-Ying Ni
} Institute of Energy Conversion
} University of Delaware
} Newark, DE 19716
**************************************
Dear Chao-Ying Ni & :
The obvious microscope related changes to consider
are: Less beam current, smaller condensor setting, smaller condensor
aperture. More important is the voltage used. Frequently a higher but
sometimes a lower kV will cause markedly less damage. The most important
reduction of a "melt-down", or other specimen beam damage is achieved
through carbon coating the specimen. The thicker the coating, the better the
specimen will tolerate the beam. Eventually this will compromise resolution.
Cheers
Jim Darley
Probing & Structure
} (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia
}
} Phone +61 77 740 370 Fax: +61 77 892 313
} A great microscopy site http://www.ultra.net.au/~pns/





From: Heike Buecking :      heibueck-at-uft.uni-bremen.de
Date: Fri, 15 Nov 1996 11:08:36 +0100
Subject: Thanks Re: cryo-substitution

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Dear microscopists,

Thanks for your advices concerning the cryo-substitution unit. For special
questions i will contact you directly.

With compliments
Heike Buecking
Dr. Heike Buecking
Universit=E4t Bremen, UFT
Physiologische Pflanzenanatomie
Leobener Str.
28359 Bremen
Tel: +49-0421-218-2954
-7283
Fax: +49-0421-218-3737





From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 15 Nov 1996 09:59:15 +0100
Subject: Re: probl. electropol.

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Message-Id: {n1364070163.17525-at-ematserv.ruca.ua.ac.be}

REGARDING RE} probl. electropol.

George has asked about contamination during electropolishing. I'm afraid
it'll be very hard to give a direct solution to his problem unless someone
has worked on exactly the same material. I think, however, this could be a
good start for a general discussion on this issue.
In the past we have also tried a number of solutions for contamination but
could not conclude on a general rule for all materials. Every case is
different. A first question is whether the oxidation or contamination is a
left-over from the polishing or occurs during later handling of the samples.
At any rate, samples should be properly washed so no acid remains on the
surface. In some cases removal of the sample from the jet apparatus with the
voltage still on has been suggested. Rapid blow drying could be of help too.
Keeping the sample in freon between thinning and EM could also limit the
amount of oxidation.
Recently the plasma-cleaning principle has been suggested and succesfully
applied for similar problems, so I heard.
We currently have similar problems with Cu-Zr samples and have a hard time to
establish the best parameters for good thinning without too much amorphous
contamination. If anyone has any suggestions here ???
Nick Schryvers






From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Fri, 15 Nov 1996 13:57:38 +0100 (MET)
Subject: Re: probl. electropol.

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On 15 Nov 1996, NICK SCHRYVERS wrote:

} REGARDING RE} probl. electropol.
}
} George has asked about contamination during electropolishing. I'm afraid
} it'll be very hard to give a direct solution to his problem unless someone
} has worked on exactly the same material. I think, however, this could be a
} good start for a general discussion on this issue.
} In the past we have also tried a number of solutions for contamination but
} could not conclude on a general rule for all materials. Every case is
} different. A first question is whether the oxidation or contamination is a
} left-over from the polishing or occurs during later handling of the samples.
} At any rate, samples should be properly washed so no acid remains on the
} surface. In some cases removal of the sample from the jet apparatus with the
} voltage still on has been suggested. Rapid blow drying could be of help too.
} Keeping the sample in freon between thinning and EM could also limit the
} amount of oxidation.
} Recently the plasma-cleaning principle has been suggested and succesfully
} applied for similar problems, so I heard.
} We currently have similar problems with Cu-Zr samples and have a hard time to
} establish the best parameters for good thinning without too much amorphous
} contamination. If anyone has any suggestions here ???
} Nick Schryvers
}
}


Maybe I mistake and the story is more complex than I imagine, but does not
aluminum oxidize very quickly (growing a passivating layer of alumina) in
air. Thus in the case of aluminum sample, as in the case George was
dealing with, if I remember, "contamination" might take place even after
polishing. Maybe a transfert in oxygen free atmosphere might be
contemplated in order to check out this point.

Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Lluis Sole i Sabaris, 1-3
E-08028 BARCELONA ESPANYA

Tel +34 (9)3 402 16 95
Fax +34 (9)3 402 13 98





From: Susanne Pignolet Brandom :      spb-at-mwrn.com
Date: Fri, 15 Nov 1996 10:19:48 -0600
Subject: trying to contact H. Nonomura

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} Date: Thu, 14 Nov 1996 15:14:57 -0800
} From: andrew berry {andrew.berry-at-flinders.edu.au}
} Reply-To: andrew.berry-at-flinders.edu.au
} To: spb-at-mwrn.com
} Subject: trying to contact H. Nonomura
}
} Hello
} I am a Actinomycete researcher in South Australia, Australia, and I
} would like to contact H. Nonomura who has published extensively in this
} area. I am co-writing a short review paper and would like to request
} permission to use some of his electronmicrographs in it.
}
} I hope you can help
} Jackie Evans
}
}
Susanne Pignolet Brandom, Ph.D.
MC Services
6-D North Commons
Lincoln MA 01773

617-259-3376

MicroWorld Resources and News
http://www.mwrn.com/






From: lfowler-at-cell-science.org (Lynn Fowler)
Date: Fri, 15 Nov 1996 10:51:59 -0500
Subject: Unsubscribe

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Please remove my name from the list. Thanks, Lynn





From: Robert Ruscica :      etp-usa-at-ix.netcom.com
Date: Fri, 15 Nov 1996 11:25:16 -0800
Subject: Variable Pressure Sem

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Message-Id: {9611151745.AA9795-at-pho903.sbphrd.com}
To: microscopy {microscopy-at-Sparc5.Microscopy.Com}
{Beverly_E_Maleeff-at-sbphrd.com}

Dear Mike,
I am sure if you contact Dr. V. Robinson at ETP-Semra, he will be able to
assist you in your search. Dr. Robinson invented the techniqe in the
1970's and will be most pleased to respond. You can reach him at
etpsemra-at-geko.net.au.
Best Regards
Robert Ruscica
ETP-USA
www.etp-usa.com
e-mail, ruscica-at-etp-usa.com




From: Susanne Pignolet Brandom :      spb-at-mwrn.com
Date: Fri, 15 Nov 1996 10:19:50 -0600
Subject: Trying to Contact A. Dietz

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Please respond to: andrew.berry-at-flinders.edu.au

} Date: Thu, 14 Nov 1996 15:19:57 -0800
} From: andrew berry {andrew.berry-at-flinders.edu.au}
} Reply-To: andrew.berry-at-flinders.edu.au
} To: spb-at-mwrn.com
} Subject: Trying to Contact A. Dietz
}
} Hello
} My name is Jackie Evans and I am an actinomycete researcher in South
} Australia, Australia. I would like to contact A.Dietz who has published
} a number of electronmicrographs of various actinomycetes. I am writing a
} short review paper and would like to ask permission to use some of these
} electronmicrographs in it.
}
} Hoping you can help me
} Jackie Evans
}
}
Susanne Pignolet Brandom, Ph.D.
MC Services
6-D North Commons
Lincoln MA 01773

617-259-3376

MicroWorld Resources and News
http://www.mwrn.com/






From: klivi-at-jhu.edu (Kenneth JT Livi)
Date: Fri, 15 Nov 1996 09:34:31 -0400 (EDT)
Subject: Re: Fume Hood - suggestions

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Though I am not an expert at fume hoods, one aspect of fume hood design
that was brought to my attention by our lab safety personnel -- were the
exhaust fan was placed. The motor needs to be at the place of exit from the
building (usually on the roof). If the fan is placed just above the lab
which is not the top floor of the building, a positive pressure builds up
in the duct above the lab and could cause leakage into the floors above (or
to the side). This is something that you would think would always be
accounted for but I am constantly surprised by engineering oversights.

Ciao for now,

Ken

} Our lab is in the process of buying a fume hood. It will be used
} primarilly for protection against EM and LM processing chemicals
} (fixatives, resins, solvents, etc.).
} My question is basically: what characteristics are recommended? (do we
} need a filter on the exhaust system? If so, what type of filter?) I would
} appreciate any suggestions.
} Thanks in advance.
}
} Adriana
}
} Adriana P. M. Rodriguez
} Biologia Celular, CENA/USP
} Av. Centenario 303, C.Postal 96
} 13400-970, Piracicaba, SP, BRAZIL
} fax 55-19-429 4610 - voice 55-19-429 4694
} e-mail {adriana-at-aguia.cena.usp.br}






From: jacobb-at-mh1.lbl.gov (Jacob Bastacky)
Date: Fri, 15 Nov 1996 09:37:56 -0800
Subject: high-resolution printers

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The LaserMaster 1800dpi printer made impressive, inexpensive prints of SEM
1 and 4 MB digital images. Better than their 1200 dpi printer and
considerably better than 600 dpi Apple. These were the first trials by the
company on images that we printed in Zuerich and here on the other two
machines.

For halftones, we've used a 2400 dpi Linotronic at the local publishing
house: $10-20 per print.


Jacob Bastacky
Room 116 Donner Laboratory
Lawrence Berkeley Laboratory EMail: sjbastacky-at-lbl.gov
University of California Phone: (510) 486-4606
Berkeley, California 94720 Fax: (510) 486-4750






From: ScottE57-at-aol.com
Date: Fri, 15 Nov 1996 09:55:22 -0500
Subject: Re: High Resolution networked Printers

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joiner-at-bcm.tmc.edu wrote:
} } The obvious solution, though not necessarily practical financially
} } speaking would be to have a reasonable laser printer for day to day
} } archiving type of work and a good dye sublimation printer to
} } be used only for those situations where publication quality is
} } required. The latter printer could be located in a institutional
} } central area and jobs sent remotely, while the laser writer would
} } be located locally. One can justify the dye sub printer costwise,
} } by comparing to the upkeep of a print darkroom. In addition, AFM
} } images and the like, for publication really cannot be output any
} } other way, not to mention the "fancy", full color transparencies
} } that can be produced!
} }
} *****************
} No, that won't work. "Day to Day" is where we need the quality. We produce a
} large number of E.M. images upon which we base medical diagnoses. These
} prints have to have and convey the maximum amount of information captured in
} the original image. The publication images are best produced in the
darkroom.

If this is the case then you should consider the Fuji Pictrography - it is
superior to the thermal dye sub technologie at a cost of $3.50 per 8.5"x11"
page or $1.75 for 1/2 page as it uses continuous material and a silver halide
one step printing process at up to 400dpi. These units make a true
photograph - following this thread unfortunatly you can not get true photo
quality at a high print speed and at a low cost per print or for the unit -
Also thermal dye sub typically is about $2.00 to $3.00 per page not $5.00 per
print.

Scott E. Berman
Advanced Imaging Concepts, Inc.
Phone(609) 921-3629 x26
Fax(609) 924-3010
e-mail Scott E57-at-aol.com





From: Wharton Sinkler :      sinkler-at-apollo.numis.nwu.edu
Date: Fri, 15 Nov 1996 08:48:10 +0200 (MEZ)
Subject: Re: probl. electropol.

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Regarding: Electropolishing

Standard jet electropolishers usually hold the 3mm disk between two pieces of
plastic. It seems to me that the time it takes to get the specimen out of the
holder (an operation which occurs at room temperature), or to rinse the acid
from the entire holder, could be a problem with extremely sensitive specimens.

One solution is to hold the specimen in a pair of platinum-tipped tweezers
(clamped closed). The specimen must be dimpled carefully, or else it will
simply dissolve in the acid before it is perforated. by viewing the specimen
through a glass beaker with a light bulb positioned behind it, it should be
possible to see the hole and quickly cut off the current before the specimen is
ruined. I have used this technique in the past to produce TEM specimens in
Titanium alloys.

Another issue is the temperature of the electrolyte. Very low temperature is
good, because any reaction between the specimen and the electrolyte, which can
occur after polishing and before cleaning, will be slowed.

A good reference on electropolishing (with references for further reading) is
written by Van der Voort, and I believe has the word "Metallography" in the
title.

Wharton

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Wharton Sinkler PhD
Department of Materials Science and Engineering
Northwestern University
2225 Sheridan Rd.
Evanston, IL 60208-3108
tel: (847) 491-7809
fax: (847) 491-7820
email: sinkler-at-apollo.numis.nwu.edu


On 15 Nov 1996, NICK SCHRYVERS wrote:

} REGARDING RE} probl. electropol.
}
} George has asked about contamination during electropolishing. I'm afraid
} it'll be very hard to give a direct solution to his problem unless someone
} has worked on exactly the same material. I think, however, this could be a
} good start for a general discussion on this issue.
} In the past we have also tried a number of solutions for contamination but
} could not conclude on a general rule for all materials. Every case is
} different. A first question is whether the oxidation or contamination is a
} left-over from the polishing or occurs during later handling of the samples.
} At any rate, samples should be properly washed so no acid remains on the
} surface. In some cases removal of the sample from the jet apparatus with the
} voltage still on has been suggested. Rapid blow drying could be of help too.
} Keeping the sample in freon between thinning and EM could also limit the
} amount of oxidation.
} Recently the plasma-cleaning principle has been suggested and succesfully
} applied for similar problems, so I heard.
} We currently have similar problems with Cu-Zr samples and have a hard time to
} establish the best parameters for good thinning without too much amorphous
} contamination. If anyone has any suggestions here ???
} Nick Schryvers
}
}







From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 15 Nov 96 13:43:36 EST
Subject: Electropolishing Aluminum Alloys

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RE: Electropolishing of Aluminum Alloys

The oxidation problem you decsribe may be caused by the delay in removing and
rinsing your sample. The nitric/methanol solution you are using will continue
to etch your sample after the unit has shut off. An alternative may be to use a
"non-Acid" electrolyte. The BK-2 solution was developed by Bernie Kestel at
Argonne National Lab and is described in his paper "Non-Acid Electrolyte Thins
Many Materials Without Hydride Formation" Ultramicroscopy 19 (1986) 205-212.

If you don't have a copy of the paper, I can FAX and/or mail one to you. I
spoke to Bernie and he said it works well on aluminum alloys, but not on pure
aluminum. He suggested a voltage of 170-200 and low temperature (-40c). You
can contact him directly at bernard_kestel-at-qmgate.anl.gov.

DISCLAIMER
The non-acid electrolyte was developed for use on a South Bay Technology Model
550 Jet Polisher. Some of the settings required (eg voltage, current and visual
observation) for use may not be available on other commercial units. We are the
manufacturer of the Model 550 and have a financial interest in promoting its
use.

I hope this information helps!

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com
http://www.southbaytech.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.

Fellow Microscopists
One of our Research students, is carrying out a Ph.D project on Al based
alloys (Spay cast 7xxx series) , (10-11% Zn, 1-1.5% Mg, 1.5-2 % Cu, 0.3
% Zr, Al balance). As part of her project she is required to carry out an
extensive TEM characterisation study of these alloys.
However, she is encountering significant problems with rapid oxidation
of the TEM samples, following preparation by a conventional twin jetting
technique (involving the use of a Struers Tenupol -3 model using an
electrolyte solution of 30% Nitric acid in methanol at -40oC).
Following preparation by twin jetting we have tried removing the oxide
films by ion beam thinning on a Gatan ion beam thinner, at a milling
angle of 10o (minimum angle permitted ) and at a voltage of 4KV and at a
current of 3mA.
Following this dual approach, some times Oxide free Aluminium foils are
prepared but some times the oxide formation persists. However, it
appears that this effect is not related to the milling time, since different
milling times were involved.
I would deeply appreciate your advise on this persistent problem, by
either their replying to the mailing list or to her directly at:
meteo-at-leeds.ac.uk

Many thanks in anticipation.

George






From: Augusto A. Morrone :      amorr-at-mse.ufl.edu
Date: Fri, 15 Nov 1996 12:11:39 -0500
Subject: Resposes on REM/RHEED in the TEM

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} Dear Netters:
} Thank you for all the responses to my question about holders to perform
} REM-RHEED in the TEM. The following is a summary of the responses I=
received.
********

Philips has a special bulk sample holder for reflection diffraction
applications (called reflection diffraction holder). It allows you to put a
bulk piece of sample onto the stage with the sample surface parallel to the
electron beam at zero tilt. You can tilt the holder a few degree to obtain
a glancing angle in order to obtain reflection diffractions. You can also
rotate the stage to obtain diffractions from different orientations.
If your sample is small enough, you can modify a double tilt holder to do
certain reflection work (see also Tung Hsu's paper: Technique of reflection
electron microscopy published in Microscopy Research and Technique
20:318-332, 1992). The thing is that you need to mount your speciman
surface to be diffracted nearly parallel to the beam.

Yi Feng, Ph.D.
Sr. Applications Specialist
Philips Electronic Instruments
85 McKee Drive
Mahwah, NJ 07430 USA

E-mail: yi_feng-at-pei.philips.com
Tel: 201 529 6405
Fax: 201 529 2252
*******************************
I am not sure what you mean by an attachment to do RHEED and REM. I
did this some time ago in a Phillips 400t; all you need is an appropriate
specimen. There are some excellent papers by Tung Hsu on the subject [e.g.
Ultramicroscopy _11_, 239, (1983), J. Vac. Sci. Tech. _B3_ (1985)] There is
also an 'idiots guide', but I don't have the reference (J. Appl. Cryst=
1983?)
Regards,

Richard Beanland,
GMMT Ltd.,
Caswell,
Towcester,
Northants NN12 8EQ
UK
*******************************
Of course it's possible to attach this attachment on your 200Cx. It's
calling HR Diff attachment because
The location is just under the projector lens (on the port you can see on
both side of column) and as the camera length is mechanically fixed
(~37cm)+=7F=1C=7F=7F=16=F5(=01=7F=7F=7F=7F=7F=7F=7F=7F=7F0*0*0*=B0=04=B0=04=
=1C=7F=7F=D4=8Cthe calculation is more accurate for
the L Lambda.
It's primary design to use with normal grid but as you can tilt at 90
degrees you can make reflection dif
on bulk sample.
This attachment is design with an airlock in order to change the sample
without breaking vacuum in camera chamber.
Depending on the tools you have in your laboratory , you can build a simple
one without airlock.
May be it's possible to find a used one. If you're interresting let me know.
Regards.

Jacky Larnould
tel 33 (0)4 67 72 28 26
fax 33 (0)4 67 79 54 90
email larnould-at-mnet.fr
*************************************

We have a RHEED (Reflection High Energy Electron Diffraction) stage
on our JEOL JEM-100CX electron microscope. It is attached under the
Projector lens (i.e. there is no lens between it and the camera), has
its own airlock, can be used both for high resolution transmission
diffraction and
for reflection diffraction and has X & Y movement, tilt and rotate
goniometer controls. We use it in reflection mode for looking at ion
implanted semi-conductors but it has also been successfully used with
surface diffusion in metals.
I also see from the JEOL catalogue for the JEM-200CX that JEOL
made a "High Resolution Electron Diffraction Stage", No AD4, for this
microscope. I assume that this is the same type of specimen stage,
since they say "transmission and reflection diffraction possible". It,
like our RHEED stage, has a fixed camera length of 312mm.
Whether this stage is still available from JEOL I cannot say -
you'd have to ask JEOL
Hope this helps you with your enquiries,

Peter Smith.
Electron Microscope Unit
Dept of App Physics
Royal Melbourne Institute of Technology
124 La Trobe St
MELBOURNE Victoria 3000
AUSTRALIA

Phone: +61 3 9660 2205
Fax: +61 3 9660 3837
*****************************************
We have one on our 100CX and routinely use it
for+=7F=1C=7F=7F=16=F5(=02=7F=7F=7F=7F=7F=7F=7F=7F=7F0*0*0*=B0=04=B0=04=1C=
=7F=7F=D4=8Cexamining thin film surfaces. In fact,
it's the
most heavily
used stage on the instrument!

The stage inserts into the column just above the projection
chamber, and has its own prepumping controls. To ensure full
rotation, the sample can be no larger than, say 0.75 cm in diameter,
although we've examined (slightly) larger samples without rotating.
Also, the samples can be up to (at least) 0.5 cm thick. Generally,
they are mounted on a stub with silver paint.

B. G. Demczyk
USAF Rome Laboratory
*****************************************
.....The important technical info in it, however, was a reference
to a chapter I wrote on reflection ED in Vol. 1 of the 2nd. Ed. of the book
'Physical Methods in Chemical Analysis', W. G. Berl, Ed published by
Academic Press. This delt with ReflED in dedicated reflection units, but I
believe the corresponding chapter in the 3rd edition deals with ED in TEMS.
Also, there is a section on Refln ED with TEMs on p. 271 of Vol 1 in the
series 'Practical Methods in Electron Microscopy', Audrey Glauert, Ed.,
North Holland, 1972 (two ISBNs given: 0-7204-4251-6 for North Holland =
&
0-444-10404-6 for American Elsevier)
W. C. Bigelow (bigelow-at-umich.edu)
**********************************
...You're right, the attachment is under the proj lens on both side, one for
the sample holder and the second one for the air lock chamber.
Take care in using a specimen holder to make REM in the objective lens cause
the tilting angle is limited to
+-30+=7F=03=C0 and in that case the holder itself create a "shadow" on your=
REM.
If you have an idea on how much you can spend on a such attachment, I can
discuss with some of my customer that doesnt use that one.
Regards.

Jacky Larnould
tel 33 (0)4 67 72 28 26
fax 33 (0)4 67 79 54 90
email larnould-at-mnet.fr
***********************************
About 10 years ago we made a reflection diffraction holder for our Jeol
100CX. We used it for a consulting job, then it went on the shelf until this
year. We got an inquiry thru Jeol and ended up selling the holder to the
Hauptman-Woodward Medical Research Institute in Buffalo, NY. The user is
Douglas Dorset.

The holder mounted on the right diffraction camera port, just above the
viewing chamber. It had two sample mounts; one for a diffraction
standard+=7F=1C=7F=7F=16=F5(=03=7F=7F=7F=7F=7F=7F=7F=7F=7F0*0*0*=B0=04=B0=04=
=1C=7F=7F=D4=8Cand one for the sample. It provided
+/- 90 deg. tilt, 360 deg. rotation and
+/- 3 mm translation in the X and Y axes. The sample holder could be
retracted out of the beam when not in use.

We still have the drawings and could build another one if anyone was=
interested.

Petyer W. Fullam
Ernest F. Fullam, Inc.
Phone: (518) 785-5533 FAX: (518) 785-8647
E-Mail: pdf-at-fullam.com

*******************************
Augusto A. Morrone
107D-MEL, P.O. Box 116400
MAIC
Materials Science and Engineering
University of Florida
Gainesville, FL 32611
(352) 392-1497 or 6985
Fax: (352) 392-0390
amorr-at-mse.ufl.edu





From: Daniel Schwartz :      dschwartz-at-mdc.com
Date: Fri, 15 Nov 1996 15:56:26 -0600
Subject: Electropolishing 7000-series aluminum alloys

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George and student:
If you are trying to prepare truly oxide-free aluminum
this will be extremely difficult, except by keeping the
material in an UHV system where you can ion mill the
oxide away and then directly examine your specimen.
However, I find that the {2nm thickness of the native
oxide that forms on clean aluminum is thin enough to
be undetectable for all but the most demanding HREM.
Under normal conditions this thin oxide layer is fully
passivating, and the specimen is quite stable.

However, if you're getting some sort of massive
oxidation throughout the entire specimen, I suspect
something is wrong with your polishing procedure. I
have a lot of experience polishing a number of alloys
in the 7000 series: 7050, 7075, 7475, using essentially
the same recipe that you have with excellent results. I
use HNO3 in methanol in a 1:2 ratio, at -40C as you did.
We have a Fishione electropolisher. It is important to
keep the specimens very clean: I rinse in 3 methanol
baths, each progressively cleaner, as immediately after
perforation as possible. I give the specimen a final
rinse in acetone to remove all the methanol and help it
dry quickly. Typically, I put the specimens into the TEM
shortly afterward, but I never have problems with
specimens that spend several days in a specimen
container.

You might try 5% perchloric acid in methanol at -40C or
lower. I've had success with it, and it may work better
for you. Unfortunately, it tends to preferentially etch
away precipitates, which I suspect you want to
examine in detail.

For metallurgists only!!
Your alloy is very high in Zn--it's the highest I've seen in
the 7000 series. What're the mechanical properties
like? Is this material something like 7093?

Dan Schwartz, McDonnell Douglas Aerospace





From: cadams-at-ibm-08.lanl.gov (Chris D. Adams)
Date: Fri, 15 Nov 1996 16:57:28 -0700
Subject: X-sec TEM of metal foils

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I have a need to do some x-sectional TEM on Ta foil specimens. I tried
sandwiching the foil between two peices of Si with M-bond 610. From
previous experience I knew the sandwich might not stick together well
enough to survive the sawing process and sure enough 87% of the samples
I cut cam apart before any grinding. I have not tried the Gatan
adhesive (G???). Does anyone have any suggestions or had similar
experiences? The samples do need to be viewed in cross-section.

Thanks in advance,

Chris Adams




From: Warren Straszheim :      wes-at-ameslab.gov
Date: Fri, 15 Nov 1996 12:21:40 -0600
Subject: Re: Image Analysis Equipment questions

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I'll take a shot at some of your questions (and then let the experts correct
me).

At 05:04 PM 11/14/96 -0600, you wrote:
} Cameras:
}
} Optronics DEI750 vs.
} Sony SODXC-970M vs.
} Pixera Professional vs.
} Kodak DCS 410 or 420 vs.
} any other suggestions?
}
} Technical question--if signal/noise ratio = 6(bits)+ 2 then how can Pixera
} Professional be 24 bit and only 46 S/N?

We have just installed a Pixera. The 24 bit image is only 8 bits on a given
color. If your formula is correct, then 6(8)+2 = 50. But since it advertises
46 dB S/N, that would mean that is really just shy of 8-bit resolution. Some
intensities are going to waffle between adjacent gray levels. (Is that the
correct wording?)

We are still gaining experience with our Pixera. The cost was nice (~$1200),
but we have yet to prove the spatial and intensity resolution. We do not
normally have rigorous applications; we thought to give the Pixera a try.

} Framegrabbers:
}
} I know Matrox and Data Translation offer good products, but which models
} will be complementary to the above cameras? Are any true RGB?

The Pixera would net need a frame grabber. They have their own interface
card for that. But you may want to get a frame buffer or arithmetic
processor board to speed up the subsequent processing.

} Computer:
}
} I have given up on trying to make do with what I have. So if I am going to
} order a new one, how does the following sound: Pentium with PCI bus, 40+
} Meg RAM, 1+gig hard drive, [good graphics card ?? how to specify?], SCSI
} port, 2 parallel ports, ZIP drive. What type of monitor is needed? And the
} big question -- Windows 95 or NT??

Sounds pretty good. I don't know if you need that 40 MB of RAM (I still work
with 16 MB), but memory is cheap now. And you are thinking about working in
color.

You will probably want a bigger hard drive. We have pretty well filled up a
4 GB drive with x-ray maps and images in a production setting. Much of that
could and should be offloaded, but it filled up quick. Disks are pretty
cheap too. A removable/re-writable drive of 300+ MB would be nice - could be
a writable CD or phase change drive, a magneto-optical, or a conventional
removable (Syquest, Zip, whatever).

Big monitors are nice for image analysis/processing. You can end up with
lots of windows open.

If Windows NT gives you a better file system, it might be worth it, but I
have no experience. I know Windows 3.x and 95 both are strapped with the 64K
chunk per disk barrier. That is, the disk is split up into (up to) 64K
pieces (allocation units) and parceled out. So a single 2 GB disk partition
would get broken up into chunks that are 32 KB in size. Thus even the
smallest files takes up 32 KB of disk. I think the NT file system gets
around that limitation. But I don't know what other complications arise
(like the availability or compatibility of programs). Someone else should
comment.
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: ken rhodes :      khodes-at-adam.com.au
Date: Sat, 16 Nov 1996 14:38:56 +1030
Subject: Unsubscribe

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Please remove my name from the list.

Thanks.
Ken





From: Jim Darley :      p&s-at-ultra.net.au
Date: Sat, 16 Nov 1996 17:37:00 +1100
Subject: Short History of ESEM (longish!)

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A Short History of the ESEM.
Recently a couple of correspondents touched on the
development of the ESEM and other variable pressure type SEMs. The history
of the development of these instruments is rather more fascinating than
most movie scripts. I do not have time to write that book, but a few
snippets are appropriate fare for microscopy.com.
In one paragraph: What is the ESEM? Specimens for SEM observation must be
preserved, dried with minimal distortion and metal coated to conduct excess
electrons to ground. The ESEM is a commercial SEM-type instrument which
employs low to no vacuum near the specimen. This is achieved through
differential pumping, vacuum-limiting apertures and very short distances
between the final aperture and the specimen. Scanning is a time-sequential
process. In the gaseous detector, similar to other types, if more electrons
emerge from the specimen at an instant in time, then greater interaction
occurs with gases in proximity and a stronger detector signal is translated
into a brighter spot on the CRT during the scanning process. Essentially, an
ESEM can look at "live", hydrated and uncoated specimens.

Viv Robinson was Gerry Danilatos' supervisor at the University of New South
Wales. Robinson was instrumental in the development of the wide-angle BS
detector which carries his name and which, in my experience, is the best BS
detector.
The early developments of the various inventions which were
crucial to the variable pressure SEM must be very largely, if not entirely,
attributed to Danilatos, who by then was working in Robinson's lab, but with
his own research grant. Later they fell out and Danilatos had a term
appointment at CSIRO's Wool Research Division. For his work he adapted an
ancient JEOL SEM which was changed beyond recognition. The instrument was
owned by the Uni of NSW but Danilatos was allowed to move it to CSIRO.
He is of Greek origin and despite the language problems he
would have had back then, he was able to obtain a PhD in physics. He was a
highly task-oriented worker and not a good mixer; his achievements were
astonishing. Still under 35 years old when his contract at CSIRO terminated,
he had over 100 publications, several patents, was an Editor for at least
one international journal - and he could not get a job anywhere!
There can be little doubt that his job prospects were
adversely affected by one or two science administrators who seemingly had
developed a penchant to actively discourage his employment by others. Why?
We can only speculate, a touch of racism, a bit of professional jealousy,
gutlessness or poor judgement.
Australia has given rise to quite a few outstanding electron microscopists.
Like the proverbial prophet, however, Danilatos is still not recognised here
as the country's most outstanding electron microscopist.
With a young family to worry about and his job at an end, he invested his
scarce funds in a trip, visiting all significant EM manufacturers around the
world to present his invention and to request that they would consider
manufacturing an SEM based on some of his patents.
The answers were variations on the theme: "Interesting, but
too limited in application, no commercial possibilities" and unspoken "but
it was not invented here". Then the break-through: AMR's management too
had turned him down, but some of the engineers were convinced of the
invention's potential. They formed a company, raised venture capital and the
ESEM was in gestation.
Danilatos became an ESEM Research Director. With that lofty
title he worked for some years in Sydney at his Bondi Beach house. The
ESEM's "ancestor" was the centrepiece in his living room. He would start up
the ancient JEOL's pump system before breakfast. Quite a few publications
were produced in that remarkable setting.
I have lost contact with Gerry Danilatos; I last saw him at
the 10th Australian EM Conference, Perth, February 1988. For all I know, he
is still labouring away at Bondi Beach, but I doubt it. There are now six
ESEM instruments in Oz. Philips bought out ESEM in 1996 and other
manufacturers make other, patent-skirting variable pressure SEMs.
No doubt others researchers have made important
contributions to the ESEM's development, but with the variable pressure
systems, several vital detector systems and the initiating of the commercial
development to his credit, Gerry Danilatos has to be regarded as Father and
Godfather of these instruments.
JK Galbraith observed that modern inventions are too complex and must be the
products of a team effort; the lonely genius is an anachronism. This is
generally true and bringing an instrument like the ESEM into production does
require a large team. However, in our times it is a singular event when one
person can make such a large contribution to the development of a very
complex instrument.
Jim Darley

PS I will make this page, perhaps slightly modified, accessible through our
Links page, see URL below.
Probing & Structure
(Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site: http://www.ultra.net.au/~pns/





From: delphi.beckman.uiuc.edu-at-delphi.beckman.uiuc.edu (steve rogers)
Date: Sat, 16 Nov 1996 14:55:13 -0500
Subject: air curtain heater

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Hi. I am looking to buy a stage heater for a multi-user facility, and have
pretty much decided on an air curtain heater. The trouble is, the only
manufacturer I've been able to find has gone out of business. Can anyone
point me in the right direction? Thanks in advance.
Steve


****************************************************
Stephen Rogers
Dept. of Cell & Structural Biology and the
Beckman Institute - Optical Visualization Facility
University of Illinois -at- C/U
srogers-at-delphi.beckman.uiuc.edu
****************************************************






From: micrick-at-earthlink.net (Rick Ellis)
Date: Sun, 17 Nov 1996 01:11:03 -0800 (PST)
Subject: Annual Xmas Mtg.

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Would appreciate information on date, time, and place (Sta. Monica?) of the
newly reorganized southern CA/"LA" microscopical society's Christmas
party/meeting Dec. 1996. Contact person?






From: Microscopy-request
Date: Friday, November 15, 1996 4:57PM
Subject: X-sec TEM of metal foils

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I have a need to do some x-sectional TEM on Ta foil specimens. I tried
sandwiching the foil between two peices of Si with M-bond 610. From
previous experience I knew the sandwich might not stick together well
enough to survive the sawing process and sure enough 87% of the samples
I cut cam apart before any grinding. I have not tried the Gatan
adhesive (G???). Does anyone have any suggestions or had similar
experiences? The samples do need to be viewed in cross-section.

Thanks in advance,

Chris Adams




From: Paul R. Perkes :      jobs-at-microscopy-online.com
Date: Sun, 17 Nov 1996 15:07:49 -0700
Subject: Marketing Postion

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Microscopy Online, a World Wide Web resource dedicated to the professional
interests of the microscopist since August 1995, is currently seeking an
assistant marketing specialist. In addition to a proven record in sales and
marketing, the potential candidate should have a general background in the
field of microscopy as well as familiarity with the WWW/electronic media.

To apply and/or obtain further information please visit our web page at
www.microscopy-online.com or contact us at jobs-at-microscopy-online.com.






From: Jim Darley :      p&s-at-ultra.net.au
Date: Mon, 18 Nov 1996 10:46:27 +1100
Subject: Variable pressure SEM- seek more history info

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Further to my posting on ESEM history: The intent was to correct the
information given in another posting and to highlight some not well known
aspects of the ESEM's history. Although I believe that that posting is true
in its essential elements, I accept that to be more useful, even a brief
history should be more broadly based. I certainly still do not want to write
"that book" but feel obliged to enlarge a bit on the development of the
variable pressure SEMs.
I ask that any additional, pertinent information is emailed to me.
Especially wanted are hard facts, dates, events and publications (I will
utilize those on the ESEM site and I have many of the old references
available).
Obviously such a history is for microscopists interest and would not change
legal facts or outcomes.
Eventually I will post a fuller (but still brief) history on this
server and post/link that history also with the LINKS of my site.
Perhaps the sciences (particularly EM) do not sufficiently value their
heritage and it is regrettable that names like: Knoll, Ruska, von Ardenne &
Oatley already would be unknown to most electron microscopists - at least
Wehnelt or Everhard & Thornly have a lasting tribute. Now may be a good time
to collect and write up the essential facts which led to the development of
the variable pressure SEM class of electron microscope.
Be assured that I am open-minded and without vested interest in these
instruments - other than that my agency sells a wide range of microscopy
supplies, some of which happen to be suitable for use with the variable
pressure SEMs.
Jim Darley
Probing & Structure
(Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site: http://www.ultra.net.au/~pns/





From: Marcel Paques :      Marcel.Paques-at-unilever.com
Date: 16/11/96 11:40
Subject: Short History of ESEM (longish!)

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Dear colleagues,

A small contribution to the "short history of ESEM".

The history of "environmental-EM" (actually ETEM) started in the Isleworth
Research Laboratory of Unilever Research in the UK. The first ETEM was designed
by J.A. Swift in 1970. In the following years G.R. Wight developed the first
ESEM at the Unilever Research Lab at Port Sunlight (UK). This work was published
in 1979.
From 1981 G.D. Danilatos started to publish on the developments of ESEM which
resulted in the introduction of a commercial instrument in 1988.

Regards,

Marcel Paques
Unilever Research Laboratory Vlaardingen
The Netherlands
e-mail: Marcel.Paques-at-unilever.com

______________________________ Reply Separator
_________________________________


A Short History of the ESEM.
Recently a couple of correspondents touched on the
development of the ESEM and other variable pressure type SEMs. The history of
the development of these instruments is rather more fascinating than most
movie scripts. I do not have time to write that book, but a few snippets are
appropriate fare for microscopy.com.
In one paragraph: What is the ESEM? Specimens for SEM observation must be
preserved, dried with minimal distortion and metal coated to conduct excess
electrons to ground. The ESEM is a commercial SEM-type instrument which
employs low to no vacuum near the specimen. This is achieved through
differential pumping, vacuum-limiting apertures and very short distances
between the final aperture and the specimen. Scanning is a time-sequential
process. In the gaseous detector, similar to other types, if more electrons
emerge from the specimen at an instant in time, then greater interaction
occurs with gases in proximity and a stronger detector signal is translated
into a brighter spot on the CRT during the scanning process. Essentially, an
ESEM can look at "live", hydrated and uncoated specimens.

Viv Robinson was Gerry Danilatos' supervisor at the University of New South
Wales. Robinson was instrumental in the development of the wide-angle BS
detector which carries his name and which, in my experience, is the best BS
detector.
The early developments of the various inventions which were
crucial to the variable pressure SEM must be very largely, if not entirely,
attributed to Danilatos, who by then was working in Robinson's lab, but with
his own research grant. Later they fell out and Danilatos had a term
appointment at CSIRO's Wool Research Division. For his work he adapted an
ancient JEOL SEM which was changed beyond recognition. The instrument was
owned by the Uni of NSW but Danilatos was allowed to move it to CSIRO.
He is of Greek origin and despite the language problems he
would have had back then, he was able to obtain a PhD in physics. He was a
highly task-oriented worker and not a good mixer; his achievements were
astonishing. Still under 35 years old when his contract at CSIRO terminated,
he had over 100 publications, several patents, was an Editor for at least one
international journal - and he could not get a job anywhere!
There can be little doubt that his job prospects were
adversely affected by one or two science administrators who seemingly had
developed a penchant to actively discourage his employment by others. Why?
We can only speculate, a touch of racism, a bit of professional jealousy,
gutlessness or poor judgement.
Australia has given rise to quite a few outstanding electron microscopists.
Like the proverbial prophet, however, Danilatos is still not recognised here
as the country's most outstanding electron microscopist.
With a young family to worry about and his job at an end, he invested his
scarce funds in a trip, visiting all significant EM manufacturers around the
world to present his invention and to request that they would consider
manufacturing an SEM based on some of his patents.
The answers were variations on the theme: "Interesting, but
too limited in application, no commercial possibilities" and unspoken "but
it was not invented here". Then the break-through: AMR's management too
had turned him down, but some of the engineers were convinced of the
invention's potential. They formed a company, raised venture capital and the
ESEM was in gestation.
Danilatos became an ESEM Research Director. With that lofty
title he worked for some years in Sydney at his Bondi Beach house. The
ESEM's "ancestor" was the centrepiece in his living room. He would start up
the ancient JEOL's pump system before breakfast. Quite a few publications
were produced in that remarkable setting.
I have lost contact with Gerry Danilatos; I last saw him at
the 10th Australian EM Conference, Perth, February 1988. For all I know, he
is still labouring away at Bondi Beach, but I doubt it. There are now six
ESEM instruments in Oz. Philips bought out ESEM in 1996 and other
manufacturers make other, patent-skirting variable pressure SEMs.
No doubt others researchers have made important
contributions to the ESEM's development, but with the variable pressure
systems, several vital detector systems and the initiating of the commercial
development to his credit, Gerry Danilatos has to be regarded as Father and
Godfather of these instruments.
JK Galbraith observed that modern inventions are too complex and must be the
products of a team effort; the lonely genius is an anachronism. This is
generally true and bringing an instrument like the ESEM into production does
require a large team. However, in our times it is a singular event when one
person can make such a large contribution to the development of a very
complex instrument.
Jim Darley

PS I will make this page, perhaps slightly modified, accessible through our
Links page, see URL below.
Probing & Structure
(Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site: http://www.ultra.net.au/~pns/




From: rac-at-unity.ncsu.edu (Robert Cushman)
Date: Mon, 18 Nov 1996 07:22:00 -0500 (EST)
Subject: Unsubscribe

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Please remove my name from the list

Bob

Bob Cushman
Department of Animal Science
313 Polk Hall
N.C.S.U.
(919)515-7232
rac-at-unity.ncsu.edu
http://www4.ncsu.edu/unity/users/r/rac/public/





From: Fred Pearson :      eoptics-at-mcmail.cis.mcmaster.ca
Date: Mon, 18 Nov 1996 09:30:29 -0500 (EST)
Subject: Re: X-sec TEM of metal foils

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Chris

You can possibly use the Gatan Epoxy ie. G1. It works very well with all
the cross-section semi-conductor samples that we prepare and cures in
about 5 minutes at about 120 C. One more thing that we do to prepare
difficult samples that fall apart because of bad substrate adherence,
is to glue a small 3 mm. MO donut type washer to one
side of the sample after the disk has been cut from the raft and just
before polishing. Dimpling is performed on the glued washer side. These
washers are available from EM. supply catalogs.

Specs. of washers is 3 mm. OD. Molybdenum 2 mm. inner hole.

If you need further info email directly.

Fred Pearson
McMaster University
email: eoptics-at-mcmaster.ca

On Fri, 15 Nov 1996, Chris D. Adams wrote:

}
} I have a need to do some x-sectional TEM on Ta foil specimens. I tried
} sandwiching the foil between two peices of Si with M-bond 610. From
} previous experience I knew the sandwich might not stick together well
} enough to survive the sawing process and sure enough 87% of the samples
} I cut cam apart before any grinding. I have not tried the Gatan
} adhesive (G???). Does anyone have any suggestions or had similar
} experiences? The samples do need to be viewed in cross-section.
}
} Thanks in advance,
}
} Chris Adams
}




From: Brian G. Demczyk :      demczyk-at-ERXINDY.rl.plh.af.mil
Date: Mon, 18 Nov 1996 11:18:00 +0001 (EST)
Subject: Low Voltage EDS Quantitative Analysis

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I wish to solicit opinions regarding the feasibility of performing
"quantitative" thin film (on substrate) EDS (including light elements)
analysis in the SEM at low (i.e. 3-5kV) accelerating voltages, using a
conventional tungsten hairpin filament electron source in a chamber with
backing pressure in the mid to upper 10^-6 Torr range.




From: Brian G. Demczyk :      demczyk-at-ERXINDY.rl.plh.af.mil
Date: Mon, 18 Nov 1996 11:18:00 +0001 (EST)
Subject: Low Voltage EDS Quantitative Analysis

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Reply to: RE} Low Voltage EDS Quantitative Analysis

You don't say what elements your thin films are to be made of, but one
fundamental requirement is that the accelerating voltage used should be from
2 to 3 times the critical excitation potential of the heaviest element being
analyzed. Unless you base your analyses on L lines, which many EDS computer
programs will not let you do, you will be very severely limited in the
elements you can do analyses for at 3 - 5 kV.

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From: m_mochel-at-uimrl7.mrl.uiuc.edu (Peggy Mochel)
Date: Mon, 18 Nov 1996 15:08:01 -0600
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From: Sunita Verma :      sverma-at-mail.arc.nasa.gov
Date: Mon, 18 Nov 1996 14:04:58 -0800 (PST)
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Sunita Verma
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From: Robb Westby :      robbwestby-at-noran.com
Date: Mon, 18 Nov 1996 15:15:37 -0600
Subject: supplies

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I am trying to find vendors to get x-ray spectroscopy supplies, in
particular, standard block materials.

I want to create my own blocks so I am looking for grains, shavings,
and/or bulk supplies

Thank you for any help

robbwestby-at-noran.com




From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Mon, 18 Nov 1996 15:44:20 -0500
Subject: sputter coaters

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Message-Id: {c=US%a=_%p=SYLVANIA%l=RD_EXC1-961118204420Z-4455-at-da-exc1.sylvania.com}

To all of those of you who sent their comments about bench-top sputter
coaters:

Thank you very much.

While no one system stood out as unconditionally superior, I found that
there was a wide variety of opinion regarding same makes/models. One
person's never-fail workhorse was another's paperweight.

Maybe I'll make my own....
-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.siemens.com
--

"Crossman, Harold" {crossman-at-RD.SYLVANIA.com}





From: paulc-at-gps.caltech.edu (Paul K. Carpenter)
Date: Wednesday, November 20, 1996
Subject: Meeting Announcement -- SCSM / MASSC

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Meeting Announcement for Southern California folks:

Southern California Society for Microscopy
and
Microbeam Analysis Society of Southern California


Location: Peppermill Restaurant, 795 E. Walnut St. Pasadena CA

Directions: Take the 210 Foothill Freeway to Pasadena and exit at Lake
Ave. Go south on Lake to Walnut St. (approx 2 blocks). Turn right on
Walnut St, the Peppermill is on the right.

5:30 pm -- Social Hour hosted by Tony Carpenter of JEOL

6:30 pm -- Dinner: Chicken breast with marsala sauce, rice pilaf, and
accompaniments (Cost $18, Students $9)

7:30 pm -- Scientific Program

Use of SEM in Determining Relationships Among Crustaceans
Jody Martin, Los Angeles Natural History Museum

Atomic Force Microscopy: Introduction and Industrial Applications.
Inga Musselman -- MAS Tour Speaker, University of Texas at Dallas.

RSVP -- Please RSVP by Friday Nov 15, 1996. Contact Paul Carpenter at
818-395-6126 or email paulc-at-gps.caltech.edu







From: becks-at-sunynassau.edu (Steve Beck)
Date: Mon, 18 Nov 1996 20:43:07 -0500
Subject: Spring 1997 - SEM Course Announcement

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SPRING 1997 COURSE ANNOUNCEMENT - Scanning Electron Microscopy (BIO. 222-E2)

NASSAU COMMUNITY COLLEGE, Garden City, Long Island, NY

A fifteen week, Spring 1997 semester, course in Biological Scanning
Electron Microscopy is being offered by the Biology Department of Nassau
Community College. This is a 4 credit course offered ONE EVENING PER WEEK,
Thursdays, starting at 5:30pm. Classes will begin on Jan. 23 and end on May
8, 1997.

This is a "hands-on" course emphasizing biological specimen preparation,
student operation of the SEM (Hitachi S-2400 recently acquired through a
NSF-ILI grant - see my website for more info.) and production of electron
micrographs through the process of black & white photography, and electron
micrograph analysis. Students will work on a variety of biological samples
with the goal of producing a portfolio of high quality SEM photomicrographs
of those samples. In addition, students will learn digital image capture,
archiving and analysis on a PowerMac 7600 using NIH Image.

The course is widely transferrable and the cost per credit is reasonable at
$78 per credit.

More information about the Bio-Imaging Center at NCC, course descriptions
and syllabi, and the humble beginnings of a student gallery of EM
photomicrographs is available at our recently completed web site. The URL
is {http://www.sunynassau.edu/webpages/biology/becks.htm} .

Interested individuals should register as soon as possible since the course
is limited to a total enrollment of ten (10) students. Registration for
students new to NCC or non-residents of Nassau County begins on December 9,
1996.

If you have further questions, you should e-mail me directly at the address
below.


Stephen J. Beck
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}






From: healthy-at-mailhost.netrunner.net (David Sontag)
Date: Mon, 18 Nov 1996 15:53:12 -0500 (EST)
Subject: Biolam -L or Leica DMLS?

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I am about to righr a check so Any comments as to this would help me out.
Which One do I Buy?

David


} Hy Victor,

} did you ever used a LOMO Biolam microscope ?

} My references are Zeiss and Wild scopes and in my opinion the LOMOs are
} optically equal. OK, the design is a left over from the 30s, with all
} advantages and disadvantages.

} + you can adjust nearly everything
} + real koehler illumination
} + you can use the scope with a mirror (field use)
} + it is extremely robust

} - you have to adjust everything
} - needs a lot of space

} The main disadvantage is you don't have a factory preset illumination, so you
} have to adjust the illumination yourself. Most biology students are not able to
} do this, so they work with a nonadjusted illumination and get a bad picture. I
} think that's the reason for the bad reputation of the LOMOs.

} So if you buy a LOMO invest at least two weeks in reading a good microscope
} book and adjust the scope yourself. Once you have properly set up yor
} microscope yourself, you achieve an excellent optically quality.

} } There is simply no comparison between the Leica DM LS and the Biolam. It's
} } like comparing a matchbox to a Porsche.

} Leica DM LS = matchbox (first position in comparison)
} Biolam = Porsche (second position in comparison)

} Another try:

} It's like comparing a fastfood menu to a real menu. :-)

} (I know Leica (is it Leitz-Cambridge or Leitz Camera ?) builds really good
} scopes, so please don't overemphasize the above comparisons)

} Carsten Pitz

Carsten

Let me get this clear : you are saying the biolam is the porsche -
that is better then the DMLS -a matchbox ? You are the first person to
give this opinion - do you own one? I am about to buy the DMLS - not a
bad scope compared to many - no plastic parts etc., but I could buf a
biolam -L for 1/2 the cost of the leica with many more option?

david





From: Mario Caron :      caron-at-lisa.polymtl.ca
Date: Mon, 18 Nov 1996 22:00:28 -0500
Subject: Re: Low Voltage EDS Quantitative Analysis

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At 11:18 96-11-18 +0001, Brian G. Demczyk wrote:
} I wish to solicit opinions regarding the feasibility of performing
} "quantitative" thin film (on substrate) EDS (including light elements)
} analysis in the SEM at low (i.e. 3-5kV) accelerating voltages, using a
} conventional tungsten hairpin filament electron source in a chamber with
} backing pressure in the mid to upper 10^-6 Torr range.
}
}

We recently validate a procedure for the determination of thickness and
composition of multilayered thin film in the SEM by EDS and Monte Carlo
simulations. To do so, we used a TiN(100nm)/Ti(10nm)/SiO2(100nm)/Si sample
in conjonction with a TiN(400nm)/Si standard. The Monte Carlo simulations,
performed with the CASINO program developped at the Universite de
Sherbrooke, are used to compute calibration curves for different thickness
and composition from which experimental K factor are fitted. The analysis
were performed for electron beam energy ranging from 2KeV to 7KeV using a
SEM Philips XL-20 (tungstene) equiped with a Link ISIS system operating in
the windowless mode. For the deconvolution of the Ti La and N Ka lines we
used also an in-house deconvolution program developped for the traitment of
EDS spectrum.

Therefore, EDS at low energy are effectively possible but they are not so
easy and as mentionned by Wil Bigelow, commercial softwares do not allow
such analysis, especially when more than one element is present in two or
more layers and when severe case of peaks convolution is involved.
Background substration is another problem for low energy. The linear fit is
not apllicable. You should also have strategies to taking into account
detector icing if you working in windowless mode.

Mario Caron
Ecole Polytechnique de Montreal
Laboratory for the Integration of Sensors and Actuators





From: etpsemra-at-geko.net.au (Viv Robinson)
Date: Tue, 19 Nov 1996 09:33:27 +1000
Subject: SEM at high chamber pressures.

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Colleagues,

Several claims have been made lately about the development of SEMs
with higher pressure in the specimen chamber, in controlled environment
conditions. These instruments were first published by myself in 1974, see
reference below. (max pressure 5 torr). They were introduced commercially
by ISI/Akashi in conjunction with ETP Semra in 1978. To the best of my
knowledge, these references predate all other references. It is this work
which has resulted in some 2,000 of these types of SEMs being sold, out
selling ESEM by a factor of about 10.

One author has suggested Danilatos is the inventor of this
technology. Below is a copy of an updated review of the development of
SEM at high chamber pressures. You will see from it, my work predates all
of his by several years. I am wondering if he ever read my papers. I
believe this work pre-dates all other successful attempts at specimens in a
SEM specimen chamber at high pressures.


A REVIEW OF THE DEVELOPMENT OF SCANNING ELECTRON
MICROSCOPY AT HIGH CHAMBER PRESSURES

Vivian Robinson,
ETP Semra Pty. Ltd.,
244 Canterbury Road,
Canterbury, NSW, 2193
Australia

Ever since electron microscopes were developed, it has been the
goal of microscopists to observe specimens in their natural state, free
from artefacts which can often be introduced through specimen preparation.
For most biological specimens, that includes the presence of water.
With a pressure of 10exp-4 torr or lower required to operate a scanning
electron microscope (SEM), liquid water, which required a pressure of above
5 torr, was clearly a problem.

Although several attempts had been made to examine hydrated
specimens in a SEM, the first published results of water imaged in a stable
and reproducible manner in the SEM, were presented at the Eighth
International Congress on Electron Microscopy in Canberra in 1974
(Robinson, 1974). This represented an increase in the pressure
capability of almost 5 orders of magnitude, from less than 10exp-4 torr, to
5 torr.

Separation of the 5 torr water vapour in the specimen chamber from
the high vacuum in the electron optics column, was achieved by using a
single differentially pumped aperture. Although attempts at using thin
films for the separation had been made, they failed because they scattered
the electrons too much, even though there was no absorption. (Perhaps that
would not pose a problem with some of the thin window materials now
available.) Calculations based upon Duschman (1949), showed that the
pressure drop across a single 100mm aperture would enable a pressure of
below 10exp-4 torr to be sustained in the gun region of the SEM, whilst a
pressure of up to 10 torr was maintained in the specimen chamber, providing
the pumping speed above the aperture was greater than 10 litre/sec.

Another problem to be overcome was how to form an image? The
conventional Everhart-Thornley (E-T) secondary electron (SE) detector,
required a pressure of less than 10-4 torr to operate. Specimen current
imaging was not considered useful because ionisation of the gas molecules
could interfere with the adsorbed current. It was decided to use a wide
angle scintillator photomultiplier backscattered electron (BSE) detector
(Robinson 1974b; 1975a). These detectors could give images with similar
signal to noise and resolution as could be achieved with an E-T SE
detector.

There was still one further problem to be overcome. How to
reduce the path the electrons travelled through the higher pressure, and
thus limit the beam scattering and associated loss of image detail? This
required two steps; lowering the final aperture to reduce the distance the
electrons had to travel; and lowering the temperature of the chamber to
make sure the water vapour was never at a higher pressure. The
description of the experimental arrangement used in a modified JEOL JSM 2
SEM, was described in greater detail in a few publications. By using a
short, less than 5mm, working distance and a cooled specimen chamber, with
the specimen surrounded by an ice and water reservoir, it was possible to
produce some good images, up to x 2,000, of specimens containing liquid
water (Robinson, 1975b; 1976a; 1976b).

This system enabled hydrated specimens to be examined at
magnifications from x100 to x2000, with the water present in a stable
liquid state. The leak rate of the water from the specimen chamber,
approximately 10exp-3 torr litre/sec., was sufficiently slow that the
specimen would remain hydrated for several hours. The use of a 100 micron
aperture and the inability to alter the position of the cross over point of
the scan coils, meant that the minimum magnification attainable was x100
times.

Whilst using this technique, it was noticed that all specimens
viewed at chamber pressures above approximately 0.1 torr, were free from
charging artefacts (Robinson, 1975c). The first explanation was that it
was due to residual water in the specimen, rendering it slightly
conducting. This was determined to be an inadequate explanation and a new
one, in terms of ionisation of the residual gas molecules, was developed.
Moncrieff et al (1978), calculated the effect of ionisation due to the
incident beam, the BSEs, the charge build up on the specimen, the SEs
accelerated by the charge buildup, the positive ions attracted to the
specimen, the SEs released by the positive ions impinging upon the
surface, and the cumulative effect of these further SEs producing more
ions. They also measured these cumulative effects and showed that the
elimination of charging artefacts was due almost exclusively to the
ionisation mentioned above. Essentially, this established that as long as
the gas could be ionised, which was a property of all gases, and the
specimen could emit SEs and BSEs, which is a property of all solid and
liquid specimens, it was possible to examine a specimen in a SEM, free from
charging artefacts, at any accelerating voltage. Should an image still
display some intensity fluctuation charging artefacts, it was merely
necessary to increase the pressure of the residual gas. This increased
the ionisation effect and charging would be eliminated every time.

One other problem was how much did the gas interfere with the
electron beam? Moncrieff et al (1979) gave a very good dissertation of
the amount of scattering of the electrons by the residual gas molecules.
They calculated the elastic and inelastic scattering from nitrogen
molecules, and compared the results with experimental observation They
showed that following a single event, an electron would be scattered tens
to thousands of microns from the original beam trajectory, and would
contribute only to a background signal, which could be removed by
subtracting away some of the DC signal level. Those electrons which had
not been scattered would continue on to form a beam diameter which had the
same Gaussian FWHM diameter as would have existed without any beam
scattering. In other words, even if 90% of the electrons in the beam were
scattered, the unscattered electrons would still form a beam with the same
diameter as if there were no scattering. 90% beam scattering did not mean
90% reduction of resolution, it resulted only in minor a minor
deterioration in attainable resolution.

By that stage, the results achieved had established parameters for
high pressure SEMs. The next task was to extend the capability to the
limits determinable from the knowledge. The results of Moncrieff et al.
(1979), showed that as the pressure was increased, shorter path lengths
between the final aperture and the specimen were necessary to keep beam
scattering to minimum and thus form an usable image. For a pressure of
50 torr, this distance was less than 0.5mm, and 50 torr became the upper
practical limit of SEM using this type of technology.

Even then, 50 torr posed great problems for a single differentially
pumped aperture. For a pressure of 50 torr to be maintained on one side
of a single aperture, with 10-4 torr on the other side, an aperture
diameter of about 13 micron was required. This placed such a severe
limitation on the minimum size of the specimen which could be examined, as
to be of little practical use. To overcome this, it was necessary to have
an intermediate pressure by using two differentially pumped apertures.
Danilatos and Robinson (1979) constructed a system having this capability
and showed that this was usable. A pressure of 50 torr was equivalent to
saturated water vapour pressure at body temperature, plus a further partial
pressure of over 10 torr, for such gases as oxygen and effectively made it
possible to examine biological specimens in conditions which were close to
those necessary to support cell motility.

By the end of 1979, researchers at The University of New South
Wales, Sydney, Australia, led by myself, had developed the following
capabilities towards imaging specimens under high vapour pressure
conditions:-

a) Established differentially pumped apertures as
adequate for pressure separation in an SEM.
b) Established the parameters for the scattering of the
electron beam by the residual gas molecules.
c) Established an upper practical working pressure limit
of 50 torr, with higher pressures producing too short
a working distance requirement.
d) Shown how the ionisation of the residual gas was
responsible for the elimination of charging
artefacts.
e) Demonstrated the ability to form images of a wide
variety of specimens under a wide variety of
pressures, with and without hydration.

Neal and Mills (1980), built this type of system in a Cambridge
Stereoscan MkII SEM and were able to obtain video images of the adsorption
of water into sponge material, as well as other effects. Again, the
pressure limitation of 5 torr meant that it had to be cooled. They gave
an extended description of environmental SEM operating conditions.
Similar results have also been achieved by others, for example Shah and
Beckett (1979).

Having established the parameter for the capability to examine
specimens at higher vapour pressures, the next step was to establish
reasons for doing it. After all, at this time, most microscopists were
intent to look at their specimens in a cleaner vacuum system and to suggest
that there were advantages to be gained from going to higher pressures was
going against known convention. However, it was the ability to look at
insulating specimens at high, above 10kV, accelerating voltages, without
charging artefacts which proved to be most valuable. This capability
occurred at pressures of approximately 0.1 torr, for most working
distances. The amount of beam scattering was generally less than 10%.
As 0.1 torr was generally greater than the partial pressure of most oils
and waxes at room temperature, this capability enabled these and most other
out gassing specimens to be examined at voltages and currents suitable for
X-ray analysis, without charging artefacts. This whole situation ,
including TEM, STEM and SEM controlled environment operation, was reviewed
in 1984 (Robinson, 1984).

Interest in this capability was generated by ETP Semra Pty Ltd
which, in 1978, manufactured a device which was initially called an
environmental cell modification. This was later changed to Charge Free
Anti-contamination System (CFAS). This device enabled the specimen
chamber to be pumped by a rotary pump to a pressure controllable between
0.05 torr and 2 torr. The aperture remained a few mm inside the final
lens and an image was formed by detecting backscattered electrons. Over
one hundred of these were sold on Akashi/ISI SEMs. By 1980, Akashi
integrated a CFAS into one of their SEMs and called the integrated system
WET SEM. Over the next few years, they sold several hundred of these
systems, mostly in Japan. Despite many years of my talking to the SEM
manufacturers outside Japan, there was very little interest in building
this type of instrument. As Akashi increased its market share by actively
promoting this technique, the other major Japanese SEM manufacturers
followed, JEOL with their LV (Low Vacuum) SEM and Hitachi with their N
(Natural) SEM. Initially their sales were limited to the Japanese market,
which was perceived as being different from other markets. However, with
continued pushing by Mr Ruscica (Electron Detectors Inc) and myself on how
these devices were promoted in Japan and how a similar approach could work
in USA, sales started slowly in USA, but soon increased rapidly. AMRAY
Inc realised the potential of this type of instrument and introduced their
ECO (Environment COntrolled) SEM in 1993. Gresham Camscan introduced
their EnVac SEM. When Leica and Zeiss amalgamated to form LEO, their
first product was their VP (Variable Pressure) SEM. Philips introduced
their CP (Controlled Pressure) SEM in 1996. RJ Lee Instruments Ltd has
released their variable pressure SEM.

By 1996, the major SEM manufacturers had all released a SEM which
had the capability to examine specimens in a controllable pressure
environment in the specimen chamber of their SEM. For some SEM companies,
it was noticed that their sales of tungsten filament SEMs were almost
exclusively due to this type of SEM. These SEMs all used a single
differentialy pumped final aperture inside the final lens as a pressure
limiting aperture and a backscattered electron detector to collect a signal
to form an image. Although exact sales of this type of microscope are not
known, sales by ETP Semra Pty Ltd, of wide angle scintillator type BSE
detectors to be included in SEMs of this capability exceed 1500. Not all
of this type of SEM are fitted with a scintillator type BSE detector, and I
am unaware of the sales of solid state detectors for this purpose. I will
leave it to the imagination of your readers to determine how many of this
type of SEM have been sold, but as a conservative guess, a figure of 2000
SEMs would not be unrealistic.

While this was occurring, Danilatos continued researching higher
pressure capabilities, attempting to image at atmospheric pressure (1981).
However, this pressure placed such a severe limitation on depth of focus
and working distance that there was no further interest in that work. He
also commenced work on a secondary electron (SE) detector capable of
operating at higher specimen chamber pressures (Danilatos, 1983). Images
obtained with this environmental SE detector have displayed approximately
the same resolution capability as those obtained with an efficient BSE
detector, from similar specimens.

Much work has been performed on the development of new types of
electron guns, for example, the LaB6 and thermal and cold field emission,
to obtain greater resolution and through that greater specimen information.
The information gained from the ability to examine specimens in their
natural state, while not as spectacularly demonstrable as the improvements
to gun, is never the less making a quiet revolution to the information
which can be achieved from the specimen. It will not be long, given a
combination of the higher brightness electron gun and improvements to
detector performance, before images from hydrated biological specimens will
show as much detail as is currently achieved from dehydrated and gold
coated specimens imaged with a conventional tungsten filament.

List of References:

Robinson V N E: A wet stage modification to a scanning electron microscope;
Electron Microscopy/1974, Proc. 8th Int. Cong., Ed. J V Sanders and D J
Goodchild, Aust. Acad. Sci., Canberra, Vol. 2 (1974a) pp 50 - 51.

Dushman S: Scientific foundations of vacuum technique; John Wiley and Sons,
New York, Ch. 2 (1949).

Robinson V N E: The construction and uses of an efficient backscattered
electron detector for scanning electron microscopy; J. Phys. E: Sci.
Instrum., Vol. 7, pp 650 - 652 (1974b).

Robinson V N E: Backscattered electron imaging; Scanning Electron
Micrscopy/1975, Symp. Proc., Ed. O Johari, IITRI, Chicago, (1975a) pp 51 -
60.

Robinson V N E: A wet stage modification to a scanning electron microscope;
J. Microscopy, Vol. 103, pp 71 - 77 (1975b).

Robinson V N E: Scanning electron microscope environmental cells; Scanning
Electron Micrscopy/1976, Vol. 1, Symp. Proc., Ed. O Johari, and I Corvin,
IITRI, Chicago (1976a) pp 91 - 100.

Robinson V N E: The examination of hydrated biological specimens in a
scanning electron microscope environmental cell; Electron Microscopy/1976,
Proc. 6th Europ. Cong., Ed. Y Ben-Shaul, Tal International, Jerusalem, Vol
2 (1976b) pp 85 - 90.

Robinson V N E: The elimination of charging artefacts in the scanning
electron microscope; J. Phys. E: Sci. Instrum., Vol. 8, pp 638 - 640
(1975c).

Moncrieff D A, Robinson V N E, Harris L B, Neutralisation of insulating
surfaces in the scanning electron microscope, J. Phys. D: Appl. Phys. Vol.
12, pp 2315 - 2325 (1978).

Moncrieff D A, Barker P R, Robinson V N E: Electron scattering by gas in
the scanning electron microscope; J. Phys. D: Appl. Phys., Vol. 12, pp 481
- 487 (1979).

Danilatos G D, Robinson V N E: Principles of scanning electron microscopy
at high specimen chamber pressures; Scanning Vol. 2, pp 72 - 82 (1979).

Neal R J, Mills A Jr: Dynamic hydration studies in an SEM; Scanning, Vol.
3, pp 292 - 300 (1980).

Shah J S, Beckett A: A preliminary evaluation of moist environment ambient
temperature scanning electron microscopy (MEATSEM); Micron, Vol. 10, pp 13
- 23 (1979).

Danilatos G D: Design and construction of an atmospheric or environmental
SEM (Part 1), Scanning Vol. 4, 9 - 20 (1981).

Danilatos G D: A gaseous detector device for an environmental SEM; Micron
and Microscopica Acta, Vol. 14, pp 41 - 52 (1983)

Robinson V N E: The examination of hydrated specimens in electron
microscopes; in Echlin P, Analysis of organic and biological surfaces, John
Wiley and Sons, New York.



Jim, now that you have read the dates of my work, don't you think
these are somewhat ahead of those published by Danilatos? If you believe
he single handedly invented the environmental microscope, please show me
some dates of work which he has published which pre date my work.

As for some of his other statements. As can be seen from the
above references, the early work on environemntal SEMs and looking at
hydrated and live specimens was almost entirely the work of VNE Robinson
and his crew and was largely finished by the time Danilatos joined my team
on ARGS grant B75/15588. He was employed on ARGS funding obtained by
myself from 3 January, 1978 until 30 June, 1981. My project under that
grant was to increase the pressure to its limits and then apply it to
biological applications. Together we extended the limit to 50 torr. At
that stage Danilatos wished to extend the results to atmospheric pressure,
while I considered that too impractical. As it was The University of New
South Wales policy to let researchers try their project, he was allowed to
explore his project on that grant. You will note that he did not
acknowledge the receipt of any grant in his paper Danilatos GD An
atmospheric scanning electron microscope, Scanning vol 3, 215 (1980).

100 papers and still under the age of 35! Perhaps he could like
to list them all. "... he adapted an ancient JEOL SEM ..." In 1980,
that JEOL JSM 2 was only 12 years old, well within the expcted active life
of a SEM. It had been used by myself to look at liquid water since 1974.
As for "science administrators who developed a penchant ...", they allowed
him to take that SEM into his post University activities, an activity of
which I do not think has been extended to anyone else. It certainly was
not extended to myself when I left The University of New South Wales.
Racism? The University of New South Wales had a long history of employing
people from many different ethnic backgrounds. Professional jealousy?
Gutlessness? Strong words! Poor judgement - well that fits someone we
know.

For your information, there are about 2,000 variable pressure SEM
systems sold through the world since their release by ISI/Akashi in
conjunction with ETP Semra Pty Ltd in 1978. They were first called
Environmental Cell Modifications (ECM), quiclkly followed by Charge Free
Anti-contamination Systems (CFAS) and WET-SEM. They operated at pressures
up to 2 torr, almost 5 orders of magnitude above the previous specimen
chamber limit of {10exp-4 torr. These were commercialy available since
1978, before Danilatos even commenced publication. In 1974 the technology
to build these to 5 torr was published, 5 years before Danilatos' first
paper. Together Danilatos and I extended this pressure to 50 torr, with
him working on a research grant I obtained. True, Danilatos did attempt
atmospheric pressure, but he failed and 50 torr is the practical limit with
todays technology. That is hardly the work of a lonely genius.

So what did Danilatos do to deserve the title of Father and
Godfather of variable pressure SEMs?

1) First to image liquid water in a stable manner in an SEM?
Not before Robinson in 1974.
2) First to image liquid water at room temperature? See
Danilatos and Robinson reference above.
3) First to image at 50 torr specimen chamber pressure? See
Danilatos and Robinson reference above?
4) First to image at atmospheric pressure? First to attempt -
full marks for trying - but the results were not satisfactory
and no one is interested in or extending the work.
5) First to commercialise SEM with high pressure in the specimen
chamber. No! ISI/Akashi/ETP Semra in 1978, to a maximum
pressure of 2 torr.
6) First to determine the effect of beam scatterering. See
Moncreiff, Barker and Robinson (1979) reference cited above.
7) First to calculate the effect of ionisation and SE and BSE
yield on charge elimination. See Moncreiff, Robinson and
Harris (1978) reference cited above.

In 1978, the scientific work was extended to 5 torr and
commercialisation of the product to 2 torr had already occurred. Those
represent a minimum of four and almost five orders of magnitude increase in
available pressure in a SEM specimen chamber. Danilatos worked with me to
extend this to 50 torr, an increase of only one order of magnitude. He
worked with ElectroScan to increase the commercial limit to 20 torr, again
an increase of only one order of magnitude over ISI/Akashi's 2 torr in
1978.

8) Developed a Gaseous SE detector. His first US patent, No
4,823,006, dated April 18, 1989, is in the name of Danilatos
and Lewis! It post dates by almost 2 years a patent
application by JS Shah, the HH Wills Physics Laboratory,
University of Bristol, GB patent No 2,186,737, dated 19
August, 1987, in which reference is made to

"... means for collecting the specimen current generated by
the electron beam from the specimen, and biassing means for
producing a substantial electric field at the surface of the
specimen ..." (Claim 1)

All his own work?

".. other manufacturers make other, patent skirting variable
pressure SEMs." ElectroScan's patent only applies to a gaseous secondary
electron detector, not to differentialy pumped aperture systems or
backscattered electron detectors, which were used in these applications
years before ElectroScan was formed as a company. As mentioned earlier,
over 2,000 of these have been sold world wide by more than six different
companies, compared to about 200 ESEMs from ElectroScan. These 2,000 were
done using a technology which extended the upper SEM chamber pressure by
some five (5) orders of magnitude, using technology developed primarily by
myself. ESEM has sold about 10% of that number and only increased the
commercially available pressure capability by 1 to 1.5 orders of magnitude.



Vivian Robinson
ETP Semra Pty Ltd






From: Carl Zeiss :      micro-at-zeiss.de
Date: Tue, 19 Nov 1996 08:41:42 +0100
Subject: Carl Zeiss 150th Anniversary TODAY!

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Message-ID: {329164B6.7889-at-zeiss.de}

Dear Microscopy Friends:

November 19, 1846 was a historic day for Carl Zeiss and microscopy. It
was on that day that the precision mechanic, Carl Zeiss, officially
opened his workshop in Jena, Germany. This was the beginning of
microscope production by the firm, Carl Zeiss.

Today, November 19, 1996, we are honored to have Chancellor Helmut Kohl
with us in Jena to help celebrate the 150th anniversary of our company.
If you can come to Jena, we cordially invite all of our friends to join
us in this celebration.
We realize that most of you cannot be with us in Jena, and therefor to
enable you to participate in this celebration, we are pleased to
announce an Internet microscopy quiz contest open to all.
If you answer all of the questions correctly, your name will be entered
in a drawing to win a brass replica of a historical Zeiss microscope.
The Internet address for this contest is:

http://www.zeiss.de/mi/competition_e/rules_e.htm

Entries must be received by April 18, 1997 and complete contest rules
can be found at this site.

Best of luck to all.

Sincerely yours

James A. Sharp
Division Manager





From: Jolanta Mesjasz-Przybylowicz :      mesjasz-at-srvnac1.nac.ac.za
Date: Tue, 19 Nov 1996 11:40:12 GMT+200
Subject: Equipment for cryo-technique

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X-PMrqc: 1




Dear Microscopist,

We planned from certain time to organize laboratory specifically for
cryo-preparation different type of biological specimens for X-ray
microanalysis. Specimens type and size varies depends of projects -
from single cells from culture to bulk sample like part of seeds or
thick cross-section from plant organs.
We are routinely use the nuclear (proton) microprobe
for quantitative elemental distribution in micro-area. The sensitivity
of the nuclear microprobe allows to measure main and trace elements
(to a level of few ppm) in the same time. All analysis are done in
high vacuum and measured specimen must be completely dry. The
preparation of sample must preserve elemental distribution without
redistribution or lost of elements.

Suddenly it is a chance to obtain and spend quickly some money on
needed equipment. We start just from the beginning because so far we
use equipment from institution around.

We plan to buy freeze-drier, cryomicrotome, equipment for high
pressure freezing and freeze-substitution.
Because of administrative restriction we have to finalize it in relatively short period of time.

We, of course, contact agents and look into catalogues, but I am very
interested in your and your colleagues experience. What are you
using in your laboratory, what you could recommend, and which model one we should avoid
rather. We would like to avoid the problems which others may have
experienced. We are interested to purchase reliable high standard
equipment.

Please respond to me directly, and if you wish I will keep all this
comments confidential.

Thanks in advance for any comments and suggestions

Best regards

Jolanta Mesjasz-Przybylowicz
************************************************************************
Dr Jolanta Mesjasz-Przybylowicz
National Accelerator Centre
P.O. Box 72
Faure 7131
South Africa
tel: 27-21-8433820
fax: 27-21-8433543
Internet: MESJASZ-at-nac.ac.za
************************************************************************




From: healthy-at-mailhost.netrunner.net (David Sontag)
Date: Tue, 19 Nov 1996 07:17:58 -0500 (EST)
Subject: Biolam -L or Leica DMLS?

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I am about to righr a check so Any comments as to this would help me out.
Which One do I Buy?

David


} Hy Victor,

} did you ever used a LOMO Biolam microscope ?

} My references are Zeiss and Wild scopes and in my opinion the LOMOs are
} optically equal. OK, the design is a left over from the 30s, with all
} advantages and disadvantages.

} + you can adjust nearly everything
} + real koehler illumination
} + you can use the scope with a mirror (field use)
} + it is extremely robust

} - you have to adjust everything
} - needs a lot of space

} The main disadvantage is you don't have a factory preset illumination, so you
} have to adjust the illumination yourself. Most biology students are not able to
} do this, so they work with a nonadjusted illumination and get a bad picture. I
} think that's the reason for the bad reputation of the LOMOs.

} So if you buy a LOMO invest at least two weeks in reading a good microscope
} book and adjust the scope yourself. Once you have properly set up yor
} microscope yourself, you achieve an excellent optically quality.

} } There is simply no comparison between the Leica DM LS and the Biolam. It's
} } like comparing a matchbox to a Porsche.

} Leica DM LS = matchbox (first position in comparison)
} Biolam = Porsche (second position in comparison)

} Another try:

} It's like comparing a fastfood menu to a real menu. :-)

} (I know Leica (is it Leitz-Cambridge or Leitz Camera ?) builds really good
} scopes, so please don't overemphasize the above comparisons)

} Carsten Pitz

Carsten

Let me get this clear : you are saying the biolam is the porsche -
that is better then the DMLS -a matchbox ? You are the first person to
give this opinion - do you own one? I am about to buy the DMLS - not a
bad scope compared to many - no plastic parts etc., but I could buf a
biolam -L for 1/2 the cost of the leica with many more option?

david





From: Michael J. Lyon :      lyonm-at-vax.cs.hscsyr.edu
Date: Tue, 19 Nov 1996 09:27:05 -0500
Subject: Rotory Shadowing

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I am currently rotary shadowing human eye lens capsule on mica sheets.
The specimens are large and very difficult to remove from the mica. We
are currently removing the replica using bleach. Can anyone suggest an
alternate method of removal or support media? We would like to stay
away from coverslips and hydrofluoric acid.

Thanks in Advance.




From: palm-at-aeetes.re.anl.gov
Date: Tue, 19 Nov 1996 09:12:40 CST
Subject: unsubscribe

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please unsubscribe me.

bob palm




From: John W Heckman :      heckman-at-pilot.msu.edu
Date: Tue, 19 Nov 1996 12:05:17 -0500 (EST)
Subject: Re: Rotory Shadowing

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Message-Id: {199611191705.MAA118490-at-pilot06.cl.msu.edu}

ML:

If you've used Pt/C to make your replicas, you might try chromic acid.
Another suggestion would be to try an enzymatic approach. I've found that for
some material enzymatic digests can beat the brute force of bleach and acid.
HF will generally work with mica to detach replicas as well as digesting mica
slurries used in quick-freeze deep-etch techniques. To just detach the
specimen, you don't need more than 10% v:v conc HF solution, not too bad if
you have a fume hood.

Good luck
John Heckman
Center for Electron Optics
MSU




From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Tue, 19 Nov 1996 11:11:49 -0600
Subject: ETP Semra manual

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Message-Id: {199611191709.LAA24498-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Does anyone have a copy of the manual for an ETP Semra Model PSM II
Robinson Detector Electronics Control Module and the Robinson detector?
I've inherited (temporarily) one of these in a malfunctioning
state, and I'd like to get it working. But there's no manual.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel *all mail:*
Microscopy
University of Illinois Station A
Rm 74 Bevier Hall PO Box 5037
905 S. Goodwin Ave. Champaign, IL 61825-5037 USA
Urbana, IL 61801
(217) 244-3145
oshel-at-ux1.cso.uiuc.edu
(217) 355-1143 home

*********** looking for a job again ***********






From: Jean-Louis Beek :      ah56-at-SOLO.PIPEX.CO.ZA
Date: Tue, 19 Nov 1996 21:56:55 +0000
Subject: please unsubscribe me.

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Message-ID: {32922D27.3096-at-solo.pipex.co.za}

please unsubscribe me.




From: Jean-Louis Beek :      ah56-at-SOLO.PIPEX.CO.ZA
Date: Tue, 19 Nov 1996 21:56:55 +0000
Subject: please unsubscribe me.

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Message-ID: {32922D27.3096-at-solo.pipex.co.za}

please unsubscribe me.




From: kszaruba-at-MMM.COM (Karen S. Zaruba)
Date: Tue, 19 Nov 1996 14:56:21 -0600
Subject: Sanyo camera part

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Does anyone know of a good source for an AC adapter for a Sanyo camera?
I tried Newark Electronics and Allied Electronic and they don't carry this
particular adapter (12V DC, 0.35 A).

I also have tried Sanyo Fisher Service Corp. out of New Jersey but they are
very frustrating to communicate with. They cannot verify that the part
they quoted me is actually of the proper amperage and voltage (all the quote
says is "AC Adaptor").

If anyone knows of a source of Sanyo parts, or of specialized electronics that
I can actually TALK to, I would very much appreciate it!

BTW, thanks very much to all those who responded to my question on image
analysis equipment! You have helped me narrow down a few more choices, and
given some good advice.

Thanks again,

Karen Zaruba
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
3M Company
3M Center 270-1S-01 Phone: 612-737-2971
St. Paul, MN 55144-1000 Fax: 736-1519

These opinions are my own and may not represent those of 3M.







From: Hal Sippel :      hsippel-at-halsippel.com
Date: Tue, 19 Nov 1996 17:21:44 -0500 (EST)
Subject: unsubscribe

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Please unsubscribe me.

Hal Sippel





From: Hal Sippel :      hsippel-at-halsippel.com
Date: Tue, 19 Nov 1996 17:22:13 -0500 (EST)
Subject: unsubscribe

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Please unsubscribe me.

Hal Sippel





From: Robert Ruscica :      etp-usa-at-ix.netcom.com
Date: Tue, 19 Nov 1996 16:04:46 -0800
Subject: Re: ETP Semra manual

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Message-ID: {32924B1E.67B5-at-ix.netcom.com}

philip oshel wrote:
}
} Does anyone have a copy of the manual for an ETP Semra Model PSM II
} Robinson Detector Electronics Control Module and the Robinson detector?
} I've inherited (temporarily) one of these in a malfunctioning
} state, and I'd like to get it working. But there's no manual.
} Phil
}
} &&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&
}
} Philip Oshel *all mail:*
} Microscopy
} University of Illinois Station A
} Rm 74 Bevier Hall PO Box 5037
} 905 S. Goodwin Ave. Champaign, IL 61825-5037 USA
} Urbana, IL 61801
} (217) 244-3145
} oshel-at-ux1.cso.uiuc.edu
} (217) 355-1143 home
}
} *********** looking for a job again ***********Philip,
If you give me the serial number of your Robinson Detector I can help you
out with a manual. You can reach me at ruscica-at-etp-usa.com or thru our
web site at www.etp-usa.com.
Regards,
Robert Ruscica




From: kennedy-at-nsi.edu (Grace Kennedy)
Date: Tue, 19 Nov 1996 16:55:51 -0800
Subject: bismuth subnitrate

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Can anyone out there provide the original reference describing the use of
bismuth subnitrate as an em stain?? Many thanks Grace






From: Hendrik O. Colijn :      colijn.1-at-osu.edu
Date: Tue, 19 Nov 1996 20:31:38 -0500 (EST)
Subject: Re: Sanyo camera part

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You should try Radio Shack. They carry a wide range of AC adapters (usually
called battery eliminators) for under $20. Many of their units have
multiple plugs so that they can fit many devices. In the worst case, you
can always cut off the existing plug and tie it to the new adapter.

Cheers, Henk


At 02:56 PM 11/19/96 -0600, you wrote:
} Does anyone know of a good source for an AC adapter for a Sanyo camera?
} I tried Newark Electronics and Allied Electronic and they don't carry this
} particular adapter (12V DC, 0.35 A).
}
} I also have tried Sanyo Fisher Service Corp. out of New Jersey but they are
} very frustrating to communicate with. They cannot verify that the part
} they quoted me is actually of the proper amperage and voltage (all the quote
} says is "AC Adaptor").
}
} If anyone knows of a source of Sanyo parts, or of specialized electronics that
} I can actually TALK to, I would very much appreciate it!
}
} BTW, thanks very much to all those who responded to my question on image
} analysis equipment! You have helped me narrow down a few more choices, and
} given some good advice.
}
} Thanks again,
}
} Karen Zaruba
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
} 3M Company
} 3M Center 270-1S-01 Phone: 612-737-2971
} St. Paul, MN 55144-1000 Fax: 736-1519
}
} These opinions are my own and may not represent those of 3M.
}
}
}
}
Hendrik O. Colijn colijn.1-at-osu.edu
Campus Electron Optics Facility 116 W. 19th Ave.
(614) 292-0674
"Nothing is as inevitable as a mistake whose time has come."





From: ghanem-solvay-at-e-mail.com
Date: Wed, 20 Nov 1996 03:50:40 EST
Subject: SEM+EDX for sale

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Dear Microscopists,

We have available for sale a Cambridge Stereoscan 250 Mark III microscope,
equipped with an Image Store device, a one-year old BSE detector (4 quadrants
from K.E. Developments) and a Link AN10000 EDX system (Be window, 10 mm2
detector). The whole instrument is about 10-11 years old but in good condition,
the microscope having been under maintenance contract.


If you're interested, please contact me directly at :


SOLVAY Research and Technology
Rue de Ransbeek 310
1120 Brussels
Belgium
phone : (32)(2)2643422
fax : (32)(2)2642055

e-mail : ghanem-solvay-at-e-mail.com

Cheers,

Antoine Ghanem


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From: A. Kent Christensen :      akc-at-umich.edu
Date: Wed, 20 Nov 1996 08:33:12 -0500 (EST)
Subject: Re: bismuth subnitrate

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Grace,

Some original bismuth references:

Albershein P, Killias U. 1963. The use of bismuth as an electron
stain for nucleic acids. J Cell Biol 17:93-103.
Locke M, Huie P. 1977. Bismuth staining for light and electron
microscopy. Tissue Cell 9:347-371.
Brown GL, Locke M. 1978. Nucleoprotein localization by bismuth
staining. Tissue Cell 10:365-388.

A more recent example is: Takeuchi IK, Takeuchi YK, 1990. Ethanol
phosphotungstic acid and bismuth staining of spermatid nucleoli in mouse
spermiogenesis. J Struct Biol 103:104-112.

I don't remember whether or not these are bismuth subnitrate.

A. Kent Christensen
University of Michigan
{akc-at-umich.edu}

-------------------------

On Tue, 19 Nov 1996, Grace Kennedy wrote:

} Can anyone out there provide the original reference describing the use of
} bismuth subnitrate as an em stain?? Many thanks Grace
}
}
}





From: Jane A. Fagerland (847) 935-0104 :      FAGERLAND.JANE-at-igate.pprd.abbott.com
Date: Wed, 20 Nov 1996 07:52:00 -0500 (CDT)
Subject: bismuth subnitrate

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Mr-Received: by mta RANDD; Relayed; Wed, 20 Nov 1996 07:58:11 -0500
Mr-Received: by mta MCM$RAND; Relayed; Wed, 20 Nov 1996 07:58:12 -0500
Mr-Received: by mta RANDD; Relayed; Wed, 20 Nov 1996 07:58:20 -0500
Alternate-Recipient: prohibited
Disclose-Recipients: prohibited
Content-Return: prohibited

I have two references in my files:

Riva A; 1974; Journal de Microscopie 19:105-108.
Ainsworth and Karnovsky; 1972; J. Histochem Cytochem 20:225-229.

I've used the Ainsworth & Karnovsky method to enhance ferritin in
epoxy-embedded tissue sections, and it worked great.

Jane A. Fagerland, Ph.D.
Dept. Microscopy and Microanalysis
Abbott Laboratories
Abbott Park IL





From: ELMA CORTINAS :      ECORTI-at-childmed.dallas.tx.us
Date: Wed, 20 Nov 1996 09:21:08 -0600
Subject: unsubscribe

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X-Mailer: Novell GroupWise 4.1

please unsubscribe

thanks,
Elma Cortinas





From: kennedy-at-nsi.edu (Grace Kennedy)
Date: Wed, 20 Nov 1996 08:15:00 -0800
Subject: bismuth/thanks

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Wow! Many thanks to all of you for the bismuth references-extremely
helpful and collectible reading. Grace






From: dlc-at-rice.edu (Daniel L. Callahan)
Date: Wed, 20 Nov 1996 10:04:21 -0600
Subject: looking for Ag sputter-target source

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Message-Id: {199611201604.KAA20726-at-owlnet.rice.edu}

Good Day all:

We are looking for a silver sputter target for a Ted Pella sputter unit
(91000 Model 3).
The target should be 57-60 mm wide and 'appropriately' thin. Ted Pella,
Inc., could neither sell us one at a reasonable price (i.e. under $700) nor
give us another source. Any help would be appreciated.

Daniel L. Callahan
Mechanical Engineering and Materials Science
Rice University

dlc-at-rice.edu
(713) 527-8101 x3572
http://www.owlnet.rice.edu:80/~dlc/





From: colijn.1-at-osu.edu (Henk Colijn)
Date: Wed, 20 Nov 1996 12:27:56 -0400
Subject: Hitachi H9000NAR available

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We currently have a Hitachi H9000NAR TEM available. The scope is a 300kV
1.8A HRTEM that has been under continuous service contract. It is in
excellent condition, but is no longer needed for our programs. Asking
price: $320,000

Henk Colijn colijn.1-at-osu.edu
Hamish Fraser fraser.3-at-osu.edu

Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility
-------------------------------------------------------------------
Prosperity is the blessing of the Old Testament; adversity is the blessing
of the New. Francis Bacon, "Of Adversity."






From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Wed, 20 Nov 1996 09:45:51 -0600
Subject: Re: bismuth subnitrate

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In message {aeb8074a08021004d8da-at-[198.147.151.19]} Grace Kennedy writes:
} Can anyone out there provide the original reference describing the use of
} bismuth subnitrate as an em stain?? Many thanks Grace
}


Grace, Here is one:

Ainsworth, S.K., Ito S., and Karnovsky, M.J. (1972). Alkaline bismuth reagent
for high resolution ultrastructural demonstration of periodic-reactive sites. J.
Histochem, Cytochem. 20, p. 995.

A bismuth stain kit, cat. # 11436, is available from Elcetron Microscopy
Sciences. I believe this is a bismuth subnitrate kit.

Gib


Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996
Its over, but not forgotten, and it was a blast!





From: Cliff Priebe :      cliff-at-hal-pc.org
Date: Wed, 20 Nov 1996 07:49:58 -0600
Subject: Re: ETP Semra manual

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Message-ID: {32930C86.1A50-at-hal-pc.org}

Robert Ruscica wrote:
}
} philip oshel wrote:
} }
} } Does anyone have a copy of the manual for an ETP Semra Model PSM II
} } Robinson Detector Electronics Control Module and the Robinson detector?
} } I've inherited (temporarily) one of these in a malfunctioning
} } state, and I'd like to get it working. But there's no manual.
} } Phil
} }
} } &&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&
} }
} } Philip Oshel *all mail:*
} } Microscopy
} } University of Illinois Station A
} } Rm 74 Bevier Hall PO Box 5037
} } 905 S. Goodwin Ave. Champaign, IL 61825-5037 USA
} } Urbana, IL 61801
} } (217) 244-3145
} } oshel-at-ux1.cso.uiuc.edu
} } (217) 355-1143 home
} }
} } *********** looking for a job again ***********Philip,
} If you give me the serial number of your Robinson Detector I can help you
} out with a manual. You can reach me at ruscica-at-etp-usa.com or thru our
} web site at http://www.etp-usa.com.
} Regards,
} Robert Ruscica






From: Chris Wang :      wangl000-at-HG.ULETH.CA
Date: Wed, 20 Nov 1996 15:10:05 +0000
Subject: Help on using Feulgen Stain for Vero cell

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Message-ID: {32931F4C.2571-at-hg.uleth.ca}

Good Day,

We are working on an african green monkey (Vero) cell, but we don't
have any success of staining them using standard Feulgen stain
procedure. Does anyone have experience staining these cells? Your
suggestion is also highly appreciated.

Best Regards,

Christopher L.C. Wang
Dept. of Bio. Sci.
Univeristy of Lethbridge
Tel: (403) 329-2210
E-mail: wangl000-at-hg.uleth.ca




From: Doug Keene :      DRK-at-shcc.org
Date: Thu, 21 Nov 1996 21:57:25 -0600 (cst)
Subject: Re: Rotory Shadowing

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You might try exposing the replicas to acetic acid vapours.
For releasing rotary shadowed replicas of molecular
suspensions, we use 1% acetic acid (a small pool in a
covered petri, with the mica in the bottom of a smaller
petri contained within the larger petri) for about 30
minutes.
----------------------
Doug Keene
DRK-at-shcc.org






From: JKulik-at-uh.edu (Joe Kulik)
Date: Wed, 20 Nov 1996 10:16:32 -0600
Subject: TEM: x-section of metallic films on MgO

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We are interested in doing cross section TEM on metallic films grown on MgO=
substrates. The films are Fe/Au and Fe/Ag multilayers. Given the=
completely different mechanical and chemical properties of the films and=
substrate, we are not sure of the best way to make cross section samples. =
We believe that standard dimpling followed by ion milling at typical angles=
and voltages would simply cause too much damage in the metal, although we=
have not tried this as of yet. Would tripod polishing be a possibility? =
(At present we do not have a tripod polisher, but we have one on order.) =
Any suggestions would be welcome.

Thanks in advance.

Joe Kulik

___________________________________________________________________

Joseph Kulik
Texas Center for Superconductivity
University of Houston
Houston Science Center
Houston, Texas 77204-5932

Tel: (713) 743-8283
=46ax: (713) 743-8281






From: Greg Strout :      gstrout-at-obsnsrv1.bio.uoknor.edu
Date: Tue, 19 Nov 1996 09:59:49 -0600
Subject: Knife sharpening

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Message-ID: {3291D975.265-at-obsnsrv1.bio.uoknor.edu}

Sometimes there are threads that you should have saved, but just didn't.
Someone called me the other day looking for a place to send her metal
knives (LM wax sectioning) for sharpening. I recall that there was a
discussion of this on the listserve not too long ago. If anyone has
saved the thread or has a list of places that provide this service I
would appreciate it very much if you would forward it to me. TIA.
--
=================================================
Greg Strout
Electron Microscopist, University Of Oklahoma
e-mail: gstrout-at-ou.edu
=================================================




From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Wed, 20 Nov 1996 11:48:11 -0600
Subject: Materials postdoctoral position

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Message-Id: {199611201737.LAA01484-at-Sparc5.Microscopy.Com}
Mime-Version: 1.0
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POSTDOCTORAL POSITION IN ELECTRON MICROSCOPY

Materials Science Division

Argonne National Laboratory


The Materials Science Division at Argonne National Laboratory has an
immediate postdoctoral opening for an electron microscopist with experience
in materials problems of interest to the Materials Science Division
emphasizing analysis of inert gases in waste storage glasses, radiation
damage and analysis of phase transformation structures and kinetics by in
situ TEM techniques. Excellent written and oral communication skills are
required. Program summaries and research highlights for all Materials
Science Division groups can be found at www.msd.anl.gov. Candidates should
have received, within the past 3 years, a Ph.D. in Physics, Materials
Science or related discipline, and are requested to send a resume, names of
references, a statement of research interests, and copies of selected
publications to Robert C. Birtcher, Materials Science Division, Argonne
National Laboratory, Argonne, Illinois 60439. Questions can be addressed
to Robert C Birtcher at (708) 252-4996, (708) 252-4798 (fax), or
Birtcher-at-anl.gov.

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(630)252-7194
FAX: (630)252-4798






From: Heather A Owen :      owenha-at-csd.uwm.edu
Date: Wed, 20 Nov 1996 14:12:48 -0600 (CST)
Subject: EDS of biological cryosections

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We are in the process of aquiring an energy dispersive analysis system
for our TEM. One of our goals is to do elemental analysis on frozen
hydrated ultrathin sections. From the reading I'm doing it appears that,
because of the vulnerability of these sections to electron beam damage,
it would be to our advantage to get an EDS system with a pulse processor
that has the highest acquisition rate possible.

Am I off base with this assumption? I'd appreciate comments from persons
with experience in this sort of thing (i.e. users, not vendors) that may
help us in the decision-making process. Please e-mail directly to me.

Thanks in advance,
Heather Owen

Heather A. Owen, Director
Electron Microscope Laboratory
Department of Biological Sciences
University of Wisconsin - Milwaukee
(414)229-6816







From: Linda L. Kirstein :      104365.3522-at-CompuServe.COM
Date: Wed, 20 Nov 1996 13:13:54 -0500
Subject: NESEM's 30th Anniversary Fall Symposium and Seminar

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The New England Society for Electron Microscopy presents their 30th Annual
Fall Symposium and Seminar
Dates: December 6th and 7th, 1996
Location: Tara Ferncroft Conference Center, 50 Ferncroft Road, Danvers, MA
[(508)777-2500 for hotel reservations only].

PROGRAM
FRIDAY, DECEMBER 6, 1996

12 noon-----Registration and Welcome

1pm-----"Nanometer Scale Spectrum-Imaging of Interfaces: Seeing Chemical
Bonds and Understanding Materials Properties" to be presented by Dr. John
Bruley, Dept of Materials Science and Engineering at Lehigh University

1:45pm-----"Confocal Microscopy: Present Capabilities and Future
Challenges" to be presented by Dr. Jim Pawley, and MSA/LAS-sponsored
speaker from the University of Wisconsin

2:30pm-----"Digital Imaging in Electron Microscopy and Its Applications to
Remote Instrument Operation and TelePresence Microscopy" to be presented by
Dr. Lawrence F. Allard from the High Temperature Materials Lab at Oak Ridge
National Laboratory

3:15pm-----Coffee Break

3:45pm-----"Imaging Muscle: From 3-D to 2-D and Back Again" to be
presented by Dr. Margaret Ann Goldstein, President of MSA, from Baylor
College of Medicine.

NESEM's Annual Business Meeting will open at 5pm, followed by our 30th
Anniversary celebration Reception and Dinner.

8:15pm-----"Image Colorzation and 3-D Effects to be presented by Dr. Robert
Anderhalt from Philips Electronic Instruments Co.

SATURDAY, DECEMBER 7, 1996
Image Processing Seminar

Dr. John Friel and Dr. Eddie Prestridge will present a seminar on image
processing and analysis relative to its use in light and electron
microsscopy. Some of topics included are: What the computer perceives in
an image; Pixels, what are they and how big are they; Common image
processing functions; and Image processing and analysis, is there a
difference?

9am-----Image Processing Seminar

9:50am-----Commercial Exhibition

10:20am------Image Analysis Seminar

11:35am-----Vendor Interactive: Participants are encouraged to bring their
favorite stored image. Image-processing programs and equipment will be
available for trial.

1pm-----Seminar Adjourns

NEW MEMBERS WELCOME!

To register, contact L. Kirstein at tel: 508-473-9673 or E-mail:
104365.3522-at-compuserve.com.
(Mark message: NESEM 30th Registration)

REGISTRATION DEADLINE: NOVEMBER 27, 1996.











From: dlc-at-rice.edu (Daniel L. Callahan)
Date: Wed, 20 Nov 1996 10:04:21 -0600
Subject: looking for Ag sputter-target source

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Reply to: RE} looking for Ag sputter-target source

Silver is reasonably easy to deposit on glass, as is done in making mirrors,
and I believe it is also readily deposited by electroplating. Any chance
you could get what you need by depositing a layer of silver on a piece of
brass shim stock, or something like that???
Good luck, Bigelow-at-umich.edu

--------------------------------------

We are looking for a silver sputter target for a Ted Pella sputter unit
(91000 Model 3).
The target should be 57-60 mm wide and 'appropriately' thin. Ted Pella,
Inc., could neither sell us one at a reasonable price (i.e. under $700) nor
give us another source. Any help would be appreciated.

Daniel L. Callahan
Mechanical Engineering and Materials Science
Rice University

dlc-at-rice.edu
(713) 527-8101 x3572
http://www.owlnet.rice.edu:80/~dlc/


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From: rw9-at-psu.edu (Rosemary A. Walsh)
Date: Wed, 20 Nov 1996 21:47:23 -0500
Subject: Re: What is a Reichert Ultra cut E worth?

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At 2:47 PM 11/7/96 -0500, larry hawkey wrote:
} We have Reichert Ultra cut E, Reichert knife breaker, (Really LKB with
} Reichert logo) and a LKB auto stainer (for grids). We have shut down our
} EM lab and my boss is thinking about selling them. Duke surplus has to
} apraise them, but these guys don't really know what these things might be
} worth. Does any one want to give me a guess as to what they might be
} worth? All are about 6 years old and are working. When we sold the scope
} there didnot seem to be much of a market, although, we did eventually sell
} it. Is it amy better for microtomes and other lab stuff?
}
} I thank you in advance for any helpful information.
}
} larry hawkey
} Former Electron Microscopist
} Department of Neurobiology
} Duke University
}
} Larry Hawkey
} hawkey-at-neuro.duke.edu
} Department of Neurobiology
} Duke University


Larry,
I am interested in learning what your surplus
department sets as the price---I saw another 5 years
ago (13+years old) for $12-$15K.
Rosemary






From: r.g.white-at-sci.monash.edu.au (Rosemary White)
Date: Thu, 21 Nov 1996 09:48:55 +1200
Subject: POSITION AVAILABLE

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POSITION: RESEARCH ASSISTANT/RESEARCH FELLOW LEVEL A

We require an experienced person to set up and manage a new multi-
institution confocal facility at Monash University, to be shared with
groups from the Victoria University of Technology and CSIRO, located in the
Department of Ecology and Evolutionary Biology. The facility will include
both upright and inverted microscopes plus a stand-alone image analysis
workstation.

Experience in use and maintenance of confocal microscope hardware and
software is essential. Experience in a broad range of confocal
applications in biology is highly desirable, so that the appointee could
assist users in selecting the best technique for their particular project,
and bring new applications to their notice on a regular basis. Familiarity
with 3D reconstruction and image analysis applications is also desirable.

The position is available for one year in the first instance, commencing
between 1 February and 1 March, 1997, at a starting salary of $30,130 to
$40,889 ($38,092 minimum with PhD) depending on experience. During 1997,
users will require training in use of the microscope, and management
protocols will be established. The appointee will be part of a small
management committee representing the major user groups.

For further details, contact Dr. David Smyth, Department of Genetics and
Developmental Biology, email David.Smyth-at-sci.monash.edu.au, ph.
61-3-9905-3861, fax 61-3-9905-5537 or Dr. Rosemary White, email
r.g.white-at-sci.monash.edu.au.

Please send applications, including CV and names of three referees, to Ms.
Annabel Carle, Department of Ecology and Evolutionary Biology, Monash
University, Clayton, Victoria 3168, Australia.

Closing date for applications is 27 December, 1996.

______________________________________________________________________
**********************************************************************

Rosemary White
Department of Ecology and Evolutionary Biology
Monash University, Clayton, Victoria 3168, Australia
phone 61-3-9905 5670 email r.g.white-at-sci.monash.edu.au
fax 61-3-9905 5613 or rgwhite-at-vaxc.cc.monash.edu.au






From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Thu, 21 Nov 1996 09:03:46 +0000
Subject: Re: looking for Ag sputter-target source

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} Good Day all:
}
} We are looking for a silver sputter target for a Ted Pella sputter unit
} (91000 Model 3).
} The target should be 57-60 mm wide and 'appropriately' thin. Ted Pella,
} Inc., could neither sell us one at a reasonable price (i.e. under $700) nor
} give us another source. Any help would be appreciated.
}
} Daniel L. Callahan
} Mechanical Engineering and Materials Science
} Rice University
}
} dlc-at-rice.edu
} (713) 527-8101 x3572
} http://www.owlnet.rice.edu:80/~dlc/

How about silver coins? I don't know if there are any that have high enough
purity - you might need to find some old ones.

Regards,

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
---------------------------------------------------------------






From: ebs-at-ebsciences.com
Date: Thu, 21 Nov 1996 07:36:20 -0600
Subject: Re: looking for Ag sputter-target source

Contents Retrieved from Microscopy Listserver Archives
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Dear Daniel and colleagues,

The Ted Pella #91000 Model 3 sputter coater was a Polaron E5000 coater in a
private label box. We (Energy Beam Sciences) are the exclusive U.S.
distributors for Polaron and we stock consumables and spare parts for this,
and all other Polaron equipment.

The correct target is a 57mm diameter foil, and silver is one of the
available target materials.

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: lkerr-at-mbl.edu (Louis Kerr)
Date: Thu, 21 Nov 1996 10:06:50 -0500
Subject: Re: Knife sharpening

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Greg,

I have used, and been very happy with:

CL Sturkey, Inc.
1549 Joel Drive
Lebanon, PA 17046
800-274-9446

Hope this helps,
Louie

Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)

VISIT OUR WEB SITE:
http://www.mbl.edu






From: chenxi-at-rmslab.rockefeller.edu
Date: Thu, 21 Nov 1996 10:16:18 EST
Subject: TEM image analysis software

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Hi,
we are trying to analyze the distribution of PECAM-1 in the junctions of
endothelial cells using TEM immunogold labelling. We are thinking of scanning
the negatives onto the computer screen, input the gold particles using
a mouse, and build a graph of gold particles in relation to the position
of the junctions (apical to basal). We would like to know: 1). What type of
scanner we can use? 2). What type of software are available for this kind
of work? 3). Any better methods?
Thanks.

Xia Chen
Laboratory of Cellular Physiology and Immunology
Rockefeller University
Tel.(212)327-7985
chenxi-at-rockvax.rockfeller.edu




From: rnessler :      rnessler-at-emiris.iaf.uiowa.edu
Date: Thu, 21 Nov 1996 10:21:44 -0500 (CDT)
Subject: Re: Knife sharpening

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On Tue, 19 Nov 1996, Greg Strout wrote:

} Sometimes there are threads that you should have saved, but just didn't.
} Someone called me the other day looking for a place to send her metal
} knives (LM wax sectioning) for sharpening. I recall that there was a
} discussion of this on the listserve not too long ago. If anyone has
} saved the thread or has a list of places that provide this service I
} would appreciate it very much if you would forward it to me. TIA.
} --
} =================================================
} Greg Strout
} Electron Microscopist, University Of Oklahoma
} e-mail: gstrout-at-ou.edu
} =================================================
}

We send our cryostat knives to Otto Anklam Co., 4824 East 42nd
Street, Minneapolis, Minn. 55406 for resharpening. Don't have his number
handy, but I'm sure information can supply it for you. I'd call for
pricing, and to discuss the use of the resharpened knife. He changes the
sharpening angle to suit the application.

Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu





From: zullo-at-helix.nih.gov
Date: Thu, 21 Nov 1996 13:21:24 -0500
Subject: my old mail address

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Message-Id: {199611211741.MAA15077-at-ns1.axs2000.net}
To: "microscopy-at-sparc5.microscopy.com" {microscopy-at-Sparc5.Microscopy.Com}

Hi,
Please note that I do not know how to close the account that you continue to
send to that is no longer valid: zullos-at-dirpc.nimh.nih.gov.
Thanks,
Steve Zullo
zullo-at-helix.nih.gov





From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Thu, 21 Nov 1996 15:37:07 -0500
Subject: Heated Optical Microscope Stage

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Message-Id: {199611211441.JAA14510-at-ns1.axs2000.net}
To: MICROSCOPY BB {Microscopy-at-Sparc5.Microscopy.Com}

Microscopists,

A collegue is looking for commercial sources of heated stages for an
optical microscope using transmitted light. He would like a cell that
can accept a sample up to 2 cm x 1 cm.

Please respond directly to him:
David Johnston
OSRAM SYLVANIA INC.
71 Cherry Hill Dr.
Beverly, MA 01915
e-mail: johnston-at-rd.sylvania.com

Thanks

-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.siemens.com
--

"Crossman, Harold" {crossman-at-RD.SYLVANIA.com}





From: hagler.herb-at-pathology.swmed.edu (Herb Hagler, Ph.D.)
Date: Thu, 21 Nov 1996 08:02:16 -0600
Subject: Re: EDS of biological cryosections

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Heather,
I am afraid that the best pulse processor in the world will not help with
the problem of EDS analysis of frozen hydrated ultrathin sections. It
would be rare to exceed 2000 counts per second from a thin biological
specimen. The real problem is the interaction of the beam with the water
in your specimen. At the beam currents necessary to get 2000 counts, the
hydrated specimen lasts for about 5-10 seconds if you are lucky. The only
alternative is to freeze dry your specimen in the microscope (-90C for 15
to 30 minutes) and then you can do x-ray analysis on the specimen after it
is "dry".

} We are in the process of aquiring an energy dispersive analysis system
} for our TEM. One of our goals is to do elemental analysis on frozen
} hydrated ultrathin sections. From the reading I'm doing it appears that,
} because of the vulnerability of these sections to electron beam damage,
} it would be to our advantage to get an EDS system with a pulse processor
} that has the highest acquisition rate possible.
}
} Am I off base with this assumption? I'd appreciate comments from persons
} with experience in this sort of thing (i.e. users, not vendors) that may
} help us in the decision-making process. Please e-mail directly to me.
}
} Thanks in advance,
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816

Herb Hagler, Ph.D.
Director of Computer-Assisted Instruction
for Southwestern Medical School
UT Southwestern Medical Center
Dallas, TX 75235-9072
New Email Address: herb.hagler-at-email.swmed.edu
http://pathcuric1.swmed.edu/
(214)648-3890 Fax(214)648-3925
----------------------------------------------
"any sufficiently advanced technology is
indistinguishable from magic"
Arthur C. Clarke
----------------------------------------------






From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 21 Nov 1996 17:22:47 -0500 (EST)
Subject: Re: looking for Ag sputter-target source

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} } We are looking for a silver sputter target for a Ted Pella sputter unit
} } (91000 Model 3).
}
} How about silver coins? I don't know if there are any that have high enough
} purity - you might need to find some old ones.
}
Dear Larry,
The pre-1965 US silver coins are 90%, the 1965-1968 half-dollars
are 30%. It's better to get silver lumps or wire. YMMV for other countries.
Yours,
Bill Tivol




From: Warren Straszheim :      wes-at-ameslab.gov
Date: Thu, 21 Nov 1996 10:32:51 -0600
Subject: Re: EDS of biological cryosections

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I don't do TEM, but I think the wrong question was asked. The issue is what
beam current would be good for your other analytical needs and how much
damage would it do to the sample. If the beam current you use for other
reasons saturates the EDS system, then you would want a system that could
handle all the x-ray's coming its way.

However, I think the normal situation is that the current has to be cranked
up to get enough x-rays for a decent analysis. Therefore you would want a
system that gives you the highest solid angle in order to give you the
highest output. That probably translates into a 30 mm^2 detector positioned
as close to the sample as possible. That might well vary among manufacturers.

I think the pulse processing features of any system on the market would
probably handle the throughput you will encounter. You will get some more
counts with the newest electronics, but the improvement would likely be
incremental compared to the collection angle. But I suppose a real TEM user
would have to confirm that.

At 02:12 PM 11/20/96 -0600, you wrote:
} We are in the process of aquiring an energy dispersive analysis system
} for our TEM. One of our goals is to do elemental analysis on frozen
} hydrated ultrathin sections. From the reading I'm doing it appears that,
} because of the vulnerability of these sections to electron beam damage,
} it would be to our advantage to get an EDS system with a pulse processor
} that has the highest acquisition rate possible.
}
} Am I off base with this assumption? I'd appreciate comments from persons
} with experience in this sort of thing (i.e. users, not vendors) that may
} help us in the decision-making process. Please e-mail directly to me.
}
} Thanks in advance,
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816
}
}
}
}
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Warren Straszheim :      wes-at-ameslab.gov
Date: Thu, 21 Nov 1996 10:32:51 -0600
Subject: Re: EDS of biological cryosections

Contents Retrieved from Microscopy Listserver Archives
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I don't do TEM, but I think the wrong question was asked. The issue is what
beam current would be good for your other analytical needs and how much
damage would it do to the sample. If the beam current you use for other
reasons saturates the EDS system, then you would want a system that could
handle all the x-ray's coming its way.

However, I think the normal situation is that the current has to be cranked
up to get enough x-rays for a decent analysis. Therefore you would want a
system that gives you the highest solid angle in order to give you the
highest output. That probably translates into a 30 mm^2 detector positioned
as close to the sample as possible. That might well vary among manufacturers.

I think the pulse processing features of any system on the market would
probably handle the throughput you will encounter. You will get some more
counts with the newest electronics, but the improvement would likely be
incremental compared to the collection angle. But I suppose a real TEM user
would have to confirm that.

At 02:12 PM 11/20/96 -0600, you wrote:
} We are in the process of aquiring an energy dispersive analysis system
} for our TEM. One of our goals is to do elemental analysis on frozen
} hydrated ultrathin sections. From the reading I'm doing it appears that,
} because of the vulnerability of these sections to electron beam damage,
} it would be to our advantage to get an EDS system with a pulse processor
} that has the highest acquisition rate possible.
}
} Am I off base with this assumption? I'd appreciate comments from persons
} with experience in this sort of thing (i.e. users, not vendors) that may
} help us in the decision-making process. Please e-mail directly to me.
}
} Thanks in advance,
} Heather Owen
}
} Heather A. Owen, Director
} Electron Microscope Laboratory
} Department of Biological Sciences
} University of Wisconsin - Milwaukee
} (414)229-6816
}
}
}
}
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: r.g.white-at-sci.monash.edu.au (Rosemary White)
Date: Fri, 22 Nov 1996 11:27:59 +1200
Subject: POSITION AVAILABLE

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POSITION: RESEARCH ASSISTANT/RESEARCH FELLOW LEVEL A

We require an experienced person to set up and manage a new multi-
institution confocal facility at Monash University, to be shared with
groups from the Victoria University of Technology and CSIRO, located in the
Department of Ecology and Evolutionary Biology. The facility will include
both upright and inverted microscopes plus a stand-alone image analysis
workstation.

Experience in use and maintenance of confocal microscope hardware and
software is essential. Experience in a broad range of confocal
applications in biology is highly desirable, so that the appointee could
assist users in selecting the best technique for their particular project,
and bring new applications to their notice on a regular basis. Familiarity
with 3D reconstruction and image analysis applications is also desirable.

The position is available for one year in the first instance, commencing
between 1 February and 1 March, 1997, at a starting salary of $30,130 to
$40,889 ($38,092 minimum with PhD) depending on experience. During 1997,
users will require training in use of the microscope, and management
protocols will be established. The appointee will be part of a small
management committee representing the major user groups.

For further details, contact Dr. David Smyth, Department of Genetics and
Developmental Biology, email David.Smyth-at-sci.monash.edu.au, ph.
61-3-9905-3861, fax 61-3-9905-5537 or Dr. Rosemary White, email
r.g.white-at-sci.monash.edu.au.

Please send applications, including CV and names of three referees, to Ms.
Annabel Carle, Department of Ecology and Evolutionary Biology, Monash
University, Clayton, Victoria 3168, Australia.

Closing date for applications is 27 December, 1996.

______________________________________________________________________
**********************************************************************

Rosemary White
Department of Ecology and Evolutionary Biology
Monash University, Clayton, Victoria 3168, Australia
phone 61-3-9905 5670 email r.g.white-at-sci.monash.edu.au
fax 61-3-9905 5613 or rgwhite-at-vaxc.cc.monash.edu.au






From: chiba-at-mimj.co.jp (Chiba Atsushi)
Date: Fri, 22 Nov 1996 11:42:13 +0900
Subject: Ring slit selection

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Hi. I'm a total novice to microscopy. And I decided to join this ML because I now have to work on microscope manuals (Japanese-English translation).

Could anyone answer my question as follows?

When a phase-contrast condenser has 3 types of built-in ring slits (annuli), how do you select one suitable for the mounted objective? You just turn the turret, set it to the required position, and one of the ring slits is selected? Or is there any other
means to do it?

TIA.

PS. Is it correct to call the annulus "ring slit" anyway? Looks like that's the way my client calls it.


Chiba Atsushi
----------------------------------------
Surname: Chiba
Sex: Male
Snailmail: MIM, 2-19-3 Sasazuka, Tokyo 151
Site: http://www.mimj.co.jp
----------------------------------------






From: B.Pirie-at-unsw.edu.au (Brian Pirie)
Date: Fri, 22 Nov 1996 15:16:04 +1000
Subject: Unsubsribe

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From: chiba-at-mimj.co.jp (Chiba Atsushi)
Date: Fri, 22 Nov 1996 15:05:10 +0900
Subject: Ring slit selection

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Message-Id: {199611220604.PAA13754-at-mail.mimj.co.jp}
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X-Mailer: Macintosh Eudora Pro Version 2.1.3-J
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Content-Type: text/plain; charset="ISO-2022-JP"

Hi. I'm a total novice to microscopy. And I decided to join this ML because I now have to work on microscope manuals (Japanese-English translation).

Could anyone answer my question as follows?

When a phase-contrast condenser has 3 types of built-in ring slits (annuli), how do you select one suitable for the mounted objective? You just turn the turret, set it to the required position, and one of the ring slits is selected? Or is there any other
means to do it?

TIA.

PS. Is it correct to call the annulus "ring slit" anyway? Looks like that's the way my client calls it.


Chiba Atsushi
----------------------------------------
Surname: Chiba
Sex: Male
Snailmail: MIM, 2-19-3 Sasazuka, Tokyo 151
Site: http://www.mimj.co.jp
----------------------------------------






From: Jim Darley :      p&s-at-ultra.net.au
Date: Fri, 22 Nov 1996 18:52:30 +1100
Subject: Re: looking for Ag sputter-target source

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At 17:22 21/11/96 -0500, you wrote:
} } } We are looking for a silver sputter target for a Ted Pella sputter unit
} } } (91000 Model 3).
} }
} } How about silver coins? I don't know if there are any that have high enough
} } purity - you might need to find some old ones.
} }
} Dear Larry,
} The pre-1965 US silver coins are 90%, the 1965-1968 half-dollars
} are 30%. It's better to get silver lumps or wire. YMMV for other countries.
} Yours,
} Bill Tivol
********************************
The place to look for silver disks is a manufacturing jeweller wholesaler.
They could be found in Yellow Pages or trade indices. Tell them what you are
after and they make it. Cost of silver is cheap when compared with gold and
the manufacturing charge should not be high either.
I trust the target is not wanted for making mirrors. Large mirrors are made
with silver solutions, but evaporated Al makes much better optical mirrors.
We have a long standing offer to give away some high purity Al wire with
orders.
Cheers
Jim Darley

Probing & Structure
(Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site: http://www.ultra.net.au/~pns/





From: jjha-at-202.30.60.166 (Jong -Jae Ha)
Date: Fri, 22 Nov 1996 18:08:32 +0900
Subject: subscribe

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subscribe(jjha-at-202.30.60.166 (Jong -Jae Ha))






From: Jim Darley :      p&s-at-ultra.net.au
Date: Fri, 22 Nov 1996 18:52:28 +1100
Subject: Re: EDS of biological cryosections

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At 08:02 21/11/96 -0600, you wrote:
} Heather,
} I am afraid that the best pulse processor in the world will not help with
} the problem of EDS analysis of frozen hydrated ultrathin sections. It
} would be rare to exceed 2000 counts per second from a thin biological
} specimen. The real problem is the interaction of the beam with the water
} in your specimen. At the beam currents necessary to get 2000 counts, the
} hydrated specimen lasts for about 5-10 seconds if you are lucky. The only
} alternative is to freeze dry your specimen in the microscope (-90C for 15
} to 30 minutes) and then you can do x-ray analysis on the specimen after it
} is "dry".
Herb Hagler
}
} } We are in the process of aquiring an energy dispersive analysis system
} } for our TEM. One of our goals is to do elemental analysis on frozen
} } hydrated ultrathin sections. From the reading I'm doing it appears that,
} } because of the vulnerability of these sections to electron beam damage,
} } it would be to our advantage to get an EDS system with a pulse processor
} } that has the highest acquisition rate possible.
} }
} } Am I off base with this assumption? I'd appreciate comments from persons
} } with experience in this sort of thing (i.e. users, not vendors) that may
} } help us in the decision-making process. Please e-mail directly to me.
} }
} } Thanks in advance,
} } Heather Owen
**************************************
Herb is quite right. Additionally, EDS has a detection limit of about 0.1%.
This increases by an order of magnitude when the water is gone. Sure, the
percentages are only relative indicators of elemental concentrations in an
biological system, but they are more likely to remain relative in dry
sections than in the hydrated state, with unknown water losses.
Cheers
Jim Darley
Probing & Structure
(Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site: http://www.ultra.net.au/~pns/





From: BreezeImag-at-aol.com
Date: Fri, 22 Nov 1996 10:02:43 -0500
Subject: Subscribe

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Message-Id: {199611221404.JAA13232-at-post-ofc01.srv.cis.pitt.edu}
To: "microscopy-at-microscopy.com" {microscopy-at-Sparc5.Microscopy.Com}

Please add me to your email address. I work Tech Support for Kevex
Instruments and could possibly help some users of this service.

Thank you,
Jim VonDemmel
1850 Lake Park Drive
Suite 105
Smyrna, GA 33080
800-495-3839
email address:
jimv-at-kevex.com





From: GARONEL-at-cliffy.polaroid.com
Date: Fri, 22 Nov 1996 09:16 -0400 (EDT)
Subject: Use of AFM as nanoindenter

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Hi!
I've been monitoring this listsever for sometime and can't remember
seeing any AFM discussions. So if there are other AFM users out there,
I would like to find out if anyone has been successful using the probe
microscope to do hardness measurements on polymeric films. I have
tried to get reproducible data and have been unsuccessful.

Thanks,

Lynne

GaroneL-at-Polaroid.com




From: rnbalduc-at-alpha.arcride.edu.ar
Date: Fri, 22 Nov 1996 13:59:17 -1356
Subject: searching SEM

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Dear Microscopysts

We are searching an used SEM for received it in donation .The SEM will be used for Research and Education in our School.
Please, we need know the model, year of fabrication, possibility of work,and other information available.
WE PAY ALL THE COSTS OF SHIPMENT.
Please, contact me for any questions.

E-mail : RNBALDUC-at-arcide.edu.ar

Address:

Fernando Balducci
Laboratory of Electron Microscopy
School of Bioengineering.
National University of Entre Rios.
C.C 57 Suc 3
Parana - Entre Rios
Argentina.





From: Manoj Misra :      Manoj.Misra-at-unilever.com
Date: 22 Nov 1996 18:03:14 Z
Subject: Image Archival

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I am setting up a data archival system for digitally captured/scanned
TEM negatives and have evaluated a few currently available image
archival softwares. I welcome comments/experiences of those who have
or are in the process of implementing such a system.

Thanks,

Manoj MISRA




From: GARONEL-at-cliffy.polaroid.com
Date: Fri, 22 Nov 1996 09:10 -0400 (EDT)
Subject: Horizontal detector on ESEM

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Message-Id: {199611221406.JAA13313-at-post-ofc01.srv.cis.pitt.edu}
To: "microscopy-at-microscopy.com" {microscopy-at-Sparc5.Microscopy.Com}

Hi Everyone!
To save money (sound familiar), we have recently placed a horizontal
EDS detector on an ESEM (Electroscan E-3) from an old 'scope. The
optimum geometry for the ESEM is not horizontal but a detector with a
30 degree snout and use of their long working distance detector. It
would be difficult to tilt the sample 30 degrees because of the very
short working distance with that detector. I am curious if there are
other users out there who are working with horizontal detectors in
their ESEM's. If so, please contact me at GaroneL-at-Polaroid.com

Thanks in advance,
Lynne




From: Wayne England :      wengland-at-ortech.on.ca
Date: Fri, 22 Nov 1996 09:09:00 -0500
Subject: rebuilt filaments

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Hello all,

We have recently noticed that the ceramic portion of our tungsten filament
units often have a discolouration around at least one of the pegs to which
the tungsten is attached. This occurs in the form of grey or brown circle
patterns around the base of the peg. Our service representative suggests
that we may want to go to using new filaments vs. rebuilt units as the
rebuilt units have a reputation for problems. We do not have a vacuum meter
on the scope though from the cleanliness of the gun chamber we believe that
there has been adequate vacuum in the area. What are the experiences out
there regarding the rebuilt units?

Wayne England
wengland-at-ortech.on.ca
ORTECH
Mississauga, Ontario




From: delannoy-at-welchlink.welch.jhu.edu (Michael Delannoy)
Date: Fri, 22 Nov 1996 13:03:30 -0500
Subject: Confocal vs Deconvolution

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To all interested parties,
I have decided to list a shorter less detailed description of the
confocal and deconvolution demos we had here at Johns Hopkins Med School
this past summer. I run a multi-user facility for the Hopkins academic
community so we were able to test a variety of samples and imaging needs.
We looked at three confocal and two deconvolution systems. Most users were
impressed with all the systems, so we had to really nit-pick to choose the
right one.
Over all the confocal systems faired better than the deconvolution
systems. This was based on final image output and acquisiton speed. The
decons were quite nice with live material but the consensus was that PMT's
did better than the CCD's.
We finally chose the Noran Oz system with the deconvolution option.
This system gave the best 3D graphics (its on an SGI) and did the least
laser damage to live cells (The AOD instead of Galvo's for laser excitation
gave the fastest scan speeds).
The xy resolution~0.4 um and xz~0.5 um (0.4 after deconvolution). We went
with a Krypton/Argon and red HeNe. If anyone wants a more detailed
comparison I will fax my original summary (no companies please). This is
based upon my personal experience with the demos, and I am not receiving any
compensation or gifts from any of the companies.

Mike Delannoy
Microscopy Facility
JHU





From: Richard Thrift :      Richard_Thrift-at-depotech.com
Date: Fri, 22 Nov 1996 13:56:57 -0800
Subject: downloading .hqx files (e.g. NIH Image) to a PC?

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X-Mailer: Novell GroupWise 4.1

Hi
I want to try something with NIH image, and I don't have a
Mac with internet access, so I downloaded
nihimage160_68.hqx via my PC. But stuffit won't extract the
program (either using a friend's Mac or using the demo
version of Executor 2.0 on my PC).

Is there something about downloading .hqx files to a PC that
causes this? Is there anything I can do that will allow this to
work, short of finding a Mac with internet access?

And incidentally, is Executor 2.0 still a good choice for a
Mac emulator for running the current version of Image on a
PC?

Thank you very much
Richard_Thrift-at-Depotech.com





From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Fri, 22 Nov 1996 15:37:16 -0600
Subject: Re: rebuilt filaments

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Message-Id: {199611222134.PAA18030-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

} Hello all,
}
} We have recently noticed that the ceramic portion of our tungsten filament
} units often have a discolouration around at least one of the pegs to which
} the tungsten is attached. This occurs in the form of grey or brown circle
} patterns around the base of the peg. Our service representative suggests
} that we may want to go to using new filaments vs. rebuilt units as the
} rebuilt units have a reputation for problems. We do not have a vacuum meter
} on the scope though from the cleanliness of the gun chamber we believe that
} there has been adequate vacuum in the area. What are the experiences out
} there regarding the rebuilt units?
}
} Wayne England

I have to agree that there are problems with rebuilt filaments. I
have noticed shorter lives and more difficults getting good saturation and
alignments. The latter two from variations in the mounting of the new
filament wires on the pegs, and perhaps the solder joints. Rebuilt
filaments also seem to drift more when new, and near the end of their
lives.
Phil

&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
Microscopy
Station A
PO Box 5037
Champaign, IL 61825-5037 USA
(217) 244-3145 days
oshel-at-ux1.cso.uiuc.edu
(217) 355-1143 home

*********** looking for a job again ***********






From: Richard_Thrift-at-depotech.com at -SMTPlink
Date: 11/22/96 1:56 PM
Subject: downloading .hqx files (e.g. NIH Image) to a PC?

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Richard,

A friend of mine has a web page with two programs that may come in handy. The
URL is http://www.wsu.edu:8080/~mic/

Going to "Updates" there is a list of goodies, two of which are unstuffit and
binhex programs for PC's (for files that were originally on a Mac). This may
help, but I haven't tried them so I can't guarantee.

If nothing else, my friend will be excited that someone besides he and I
accessed his web page!

Cheers,

John Vetrano
_______________________________________________________________________________

Hi
I want to try something with NIH image, and I don't have a
Mac with internet access, so I downloaded
nihimage160_68.hqx via my PC. But stuffit won't extract the
program (either using a friend's Mac or using the demo
version of Executor 2.0 on my PC).

Is there something about downloading .hqx files to a PC that
causes this? Is there anything I can do that will allow this to
work, short of finding a Mac with internet access?

And incidentally, is Executor 2.0 still a good choice for a
Mac emulator for running the current version of Image on a
PC?

Thank you very much
Richard_Thrift-at-Depotech.com




From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 22 Nov 1996 16:29:51 -0500 (EST)
Subject: Re: rebuilt filaments

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}
} We have recently noticed that the ceramic portion of our tungsten filament
} units often have a discolouration around at least one of the pegs to which
} the tungsten is attached. This occurs in the form of grey or brown circle
} patterns around the base of the peg. Our service representative suggests
} that we may want to go to using new filaments vs. rebuilt units as the
} rebuilt units have a reputation for problems. We do not have a vacuum meter
} on the scope though from the cleanliness of the gun chamber we believe that
} there has been adequate vacuum in the area. What are the experiences out
} there regarding the rebuilt units?
}
Dear Wayne,
We have used rebuilt tungsten filaments without problems. We got
them from Energy Beam Sciences (I have no financial interest in EBS; I'm
just a customer). We have gotten up to several hundred hours of stable
beam from these filaments--as good as from new ones. Good luck.
Yours,
Bill Tivol




From: jbpawley-at-facstaff.wisc.edu (James Pawley)
Date: Fri, 22 Nov 1996 19:45:36 -0500
Subject: Second "3D MICROSCOPY OF LIVING CELLS" Course, Announcement

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"Simon Watkins" {swatkins-at-pitt.edu}
X-Mailer: Mail*Link SMTP/QM 3.0.0GM

Reply to: RE} optics and glass exhibit

The MSA has a committee that has been active in developing educational
programs on all kinds of microscopy, perhaps they could give you some ideas.
I believe the current committee chair is JoAn Hudson at Clemson University
(hjoan-at-clemson.clemson.edu).

--------------------------------------

Hi folks:

I have been asked for to help design an "interactive exhibit" which
demonstrates in a fairly low tech way how glass is used in optics..... This
is part of a large exhibit on the history of glass making in pittsburgh
(believe it or not, historically one of the glass making capitals in the US)
. The interactive should be simple to understand and FUN.... Your ideas on
content and interactives would be appreciated.

I look forward to being swamped with suggestions and themes

Thanks

Simon


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To: "microscopy-at-microscopy.com" {microscopy-at-Sparc5.Microscopy.Com}

Hello all,

The First Annual Course on 3D Microscopy of Living Cells was held last
summer at the University of British Columbia, in Vancouver, Canada.
Twenty-eight students got together with eleven, 3D microscopy setups and a
faculty of over 20 and we all really learned a lot in eight days. As a
result, we are going to do it again and a complete description is attached
below.

The main differences from last year will be

- More time (10 days vs. 7.5) with the extra time given to imaging theory,
time-lapse recordings and use of live-cell chambers.

- Different program of lab projects: 5 living-cell projects will be spread
over 8 setups on 4 afternoons.

- A separate 3-day Workshop, after the course, concentrated on Computer
Image processing for measurement and display of 3D data sets.

- Different month, June 19-29 rather than end on July.

Things that will stay the same:

- Focus on group teaching in interest-groups of three or four.

- Selective registration based on detailed application forms.

- "Bring-your-own" live-cell project to be done in evening sessions with
assistance from the course faculty.

- Presentations of group results on the last morning.

More complete information follows,

Thanks,

Jim Pawley


____________


Announcing a 10-Day Short Course on

3D Microscopy of Living Cells

June 19 - 29, 1997

and NEW post-course workshop on

3D Image Processing
June 30- July 2

in association with the
BioSciences Microscopy Facility
and the
Department of Computer Science
University of British Columbia
Vancouver, BC, Canada

Organized by Prof. James Pawley
University of Wisconsin-Madison
APPLICATIONS

Applicants will complete a questionnaire to assess knowledge level and
field of interest. Enrollment will be limited to about 20 participants.
Selection will be made on the basis of background and perceived need.
Those without previous LM experience will be provided with basic texts to
read before the course begins. Application packages may be obtained from:

Prof. James Pawley, Rm. 1235,
1500 Johnson Dr., Madison, WI, USA 53706.
Phone: 1-608-263-3147, Fax 1-608-265-5315,
Email: jbpawley-at-facstaff.wisc.edu

or more readily from:
http://www.cs.ubc.ca/spider/ladic/course/bulletin.html

Application deadlines:

Application forms requesting information on field of interest and level
of experience must be received for screening by March 1, 1997.
Successful applicants will be notified by April 1, and a deposit of 50%
must be received by April 15, 1997. In general, refunds of the deposit
will not be possible. The remainder is due before registration.

DATES:

Applications must be received by Mar. 1/97
50% deposit due Apr. 15/97
Registration 8:00 - 10:00 am Friday June 19/97
Last class will end with lunch Sun., June 29/97
3D Image Processing Workshop June 30- July 2

****************************************
Prof. James B. Pawley, Ph. 608-263-3147
Room 1235, Engineering Research Building, FAX 608-265-5315
1500 Engineering Dr., Madison, WI, 53706
JBPAWLEY-at-FACSTAFF.WISC.EDU






From: yonder-at-UNM.EDU
Date: Fri, 22 Nov 1996 17:02:23 -0700
Subject: Job Posting

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--========================_36905962==_
Content-Type: text/plain; charset="us-ascii"

Please post the attached job posting through 12-20-96. It is in Word 6.0

Thank you,

Bill Kroenke
Associate Director



--========================_36905962==_
Content-Type: application/mac-binhex40; name="Manager_SPCF.doc"
Content-Disposition: attachment; filename="Manager_SPCF.doc"

(This file must be converted with BinHex 4.0)



--========================_36905962==_
Content-Type: text/plain; charset="us-ascii"




*************************************

Bill Kroenke, Associate Director
Center for Micro-Engineered Materials
Farris Engineering Center Room 203
University of New Mexico
Albuquerque, NM 87131
(505)277-6824
(505)277-1024 Fax
yonder-at-unm.edu

*************************************



--========================_36905962==_--





From: Mark Yeadon :      yeadon-at-uimrl7.mrl.uiuc.edu
Date: Sat, 23 Nov 1996 16:43:20 -0600 (CST)
Subject: Re: Problem in JEOL's TEM

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Dear Suzan,

I maintain a JEOL 200CX (~15 yrs old) and have encountered similar problems
with the HT.
The problem is intermittent, occuring about once every couple of months. I
have talked to
several JEOL engineers, we checked water flow and freon levels, everything
is normal. Although
the microscope is heavily modified for UHV and in-situ sputtering
experiments I have
suspected that the problem lies somewhere in the microscope logic circuits,
giving rise to
occasional glitches. We've spent several hours tracing through circuit
diagrams etc. but
the phenomenon remains a mystery... I usually resort to re-starting the
microscope
(emergency stop, restart, then override the diffusion pump timer),
everything returns
immediately to normal.

We also had problems recently with the ready light extinguishing (during
operation as
well as when idle), and identified the penning gauge (reading ~gun pressure)
as the
culprit; below ~10-5T it shuts off the ready light which in turn shuts down
the HT. That
problem was quickly solved by cleaning the penning head.

I too would be glad to hear from anyone who may have solved the HT problem...

Faithfully,

Mark Yeadon

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%

Mark Yeadon PhD
Materials Reseach Laboratory
University of Illinois at Urbana-Champaign
Urbana, IL 61801 USA

Tel. (217) 333 2514
FAX (217) 244 2278

email: myeadon-at-uiuc.edu





From: Zhiyu Wang :      zhiyu-at-hawaii.edu
Date: Sat, 23 Nov 1996 18:44:18 -1000
Subject: Re: Problem in JEOL's TEM

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I had the same problem when I operated a JEOL-1200EX/ASID10. I my case,
the problem caused by intermittent malfunctioned DP heater. You can try
to move two temperature sensors from DP1 and DP2 to the same DP, if the
problem is still, move both to other DP. This may help you to locate
which one is wrong. I was tald that the problem may also caused by the
sensor of RP which is to measure the pumping speed of RP. If belt tension
is below certain value, the pumping speed may be low and the HV will be
shut off.

Zhiyu Wang
Department of Biosystem Engineering
University of Hawaii
Honolulu Hawaii 96822


On Sat, 23 Nov 1996 psizrk-at-biruni.erum.com.pk wrote:

}
} We are facing problem in JEOL's TEM, model: JEM-100SX that during normal
} operation (Image observation), the HT suddenly cuts off and then it cannot be
} generated again, however HT ready light remains ON. Service personnels have
} checked the system and suspects that this is probably due to insufficient
} cooling water flow through lens (column side). Now the status is rather
} intermittent that sometime the E/M. works normally but sometime and most of the
} time the same problem/phenomenon appears.
}
} Needs advise on what could be the real fault and what is remedy? Can anybody
} help??
}
} Suzan James
}





From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Sat, 23 Nov 1996 09:12:56 +0000
Subject: Re: downloading .hqx files (e.g. NIH Image) to a PC?

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X-Sender: (Unverified)
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Microscopy-at-Sparc5.Microscopy.Com

} Hi
} I want to try something with NIH image, and I don't have a
} Mac with internet access, so I downloaded
} nihimage160_68.hqx via my PC. But stuffit won't extract the
} program (either using a friend's Mac or using the demo
} version of Executor 2.0 on my PC).
}
} Is there something about downloading .hqx files to a PC that
} causes this? Is there anything I can do that will allow this to
} work, short of finding a Mac with internet access?
}
} And incidentally, is Executor 2.0 still a good choice for a
} Mac emulator for running the current version of Image on a
} PC?
}
} Thank you very much
} Richard_Thrift-at-Depotech.com

My first guess would be that the Macs think it is still a PC file and,
quite sensibily, don't like it :)

The first thing I'd do would be to go in with an application, such as
Norton Disk Editor, that can see and change the various file attibutes.
Then you need to set FILE TYPE to TEXT and CREATOR to BNHQ. Stuffit should
then recognise it.

Regards,
Larry Stoter






From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Sun, 24 Nov 1996 10:06:12 +0000
Subject: Re: Problem in JEOL's TEM

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} We are facing problem in JEOL's TEM, model: JEM-100SX that during normal
} operation (Image observation), the HT suddenly cuts off and then it cannot be
} generated again, however HT ready light remains ON. Service personnels have
} checked the system and suspects that this is probably due to insufficient
} cooling water flow through lens (column side). Now the status is rather
} intermittent that sometime the E/M. works normally but sometime and most
} of the
} time the same problem/phenomenon appears.
}
} Needs advise on what could be the real fault and what is remedy? Can anybody
} help??
}
} Suzan James

I'm not familiar with this particular model of TEM. However, the usual
design approach to saftey interlocks is to have a number of sensors,
connected in series, to a circuit holding the intended operating feature
ON. If any of the sensors detects a deviation from the expected level of
the parameter that sensor is monitoring, the circuit is broken, and the
operation go OFF.

As other replies to the list indicate, a number of parameters appear to be
monitored in the JEOL 100SX to determine if the HT should be ON or OFF.
Others that are typically in the circuit, but may not be, are, for example,
simple switches to detect that the gun chamber and/or camera chamber are
not open.

Also note that rather than a specific, separate sensor, it is possible to
use the device itself. For example, diff pump heaters can actually be in
the circuit, so that if they go open circuit (and hence the vacuum is not
functioning), the interlock circuit trips.

So, the first step is to try to obtain the diagram for the HT safety
interlock circuit and identify all the sensors and devices that might be
involved - it could quite easily be 10 or more.

From the practical side, long experience has taught me (finally) that in
situations like this, where a fault is intermittent, that the problem is
often not in any of the 'devices' or electronics making up the circuit but
the wiring and connectors. I once spent two weeks trying to locate an
intermittent fault on a plate numbering system. After removing and checking
every circuit board and all the discrete devices, I finally found the fault
in a connector - when the outer insulation of a multicored cable had been
stripped to assemble the plug, the inner insulation had been nicked and two
wires were intermittently shorting. So, it was actually a factory assembly
fault that only became apparent in the instrument after 2 years!

Hope that helps, best of luck.

Regards,

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
---------------------------------------------------------------






From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Sat, 23 Nov 1996 09:13:49 +0000
Subject: Re: Silver targets/sputter coaters

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} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

snips

} With regard to high purity aluminum wire, the aluminum one gets from
} "balling up" ordinary aluminum foil from the super market shelf is more than
} adequate for the needs of most persons evaporating Al in an EM lab. Since
} one needs quite high purity even to "roll" the aluminum into a foil in the
} first place, it is generally thought that if it can be rolled, then it is
} more than pure enough for an EM application. I have forgotten exactly what
} that purity is generally perceived to be, but if my memory is correct, it is
} something like 99.99%!
}
} Chuck
}
}
} ======================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

I agree that Al foil from the supermarket is high purity. However, most
such foil that I have seen has a 'dull' side and a 'shiny' side - I
understand that the dull side is simply oxide, but the shiny side stays
that way because of a polymer coating - why it's polymer coated, I've no
idea.

Does anyone know it this polymer coat causes any problems when the foil is
used for evaporation? I guess it is very thin, but ....

Regards,

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
---------------------------------------------------------------






From: manuela :      manuelap-at-petermac.unimelb.edu.au
Date: Mon, 25 Nov 1996 12:15:47 +1100
Subject: yeast cells

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Message-Id: {199611242041.PAA15046-at-ns1.axs2000.net}
To: MICROSCOPY BB {Microscopy-at-Sparc5.Microscopy.Com}

Could anyone help me with a protocol for processing yeast cells? I haven't
processed yeast before and want to know if there is a particular method that
works well.
I would like to embed them into spurrs resin. Any protocols and/or
references will be appreciated.

Many thanks

Manuela Palatsides.






From: manuela :      manuelap-at-petermac.unimelb.edu.au
Date: Mon, 25 Nov 1996 12:15:47 +1100
Subject: yeast cells

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Could anyone help me with a protocol for processing yeast cells? I haven't
processed yeast before and want to know if there is a particular method that
works well.
I would like to embed them into spurrs resin. Any protocols and/or
references will be appreciated.

Many thanks

Manuela Palatsides.






From: Robert D. Arnold :      arnoldr-at-labs.wyeth.com
Date: Mon, 25 Nov 1996 08:01:43 -0500
Subject: subscribe

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Message-Id: {s29952a7.038-at-labs.wyeth.com}
X-Mailer: Novell GroupWise 4.1

subscribe Microscopy arnoldr-at-war.wyeth.com





From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Mon, 25 Nov 1996 08:50:51 EST
Subject: Re: Problem in JEOL's TEM

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I had a similar problem in a JEOL 100-S several years ago. After
many service calls, the problem was finally isolated to cooling water
flow. Our recirculator water had become contaminated and plugged up
several lines, causing the problem to persist even when the water was
changed. It finally resulted in much disassembly and cleaning the
lines out with compressed air. The problem could probably be
entirely avoided with the use of additives such as antifreeze to the
coolant, but JEOL service does not like to do this.



-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Norman Elliott :      nee-at-lanl.gov
Date: Mon, 25 Nov 1996 08:02:39 -0700
Subject: Re: rebuilt filaments

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Message-Id: {3.0.32.19961125080238.0068e1b8-at-mst.lanl.gov}
X-Sender: nee-at-mst.lanl.gov
X-Mailer: Windows Eudora Pro Version 3.0 (32)

Wayne and others,
This discussion has occurred before on this list and I believe that there
was some differences in opinion at that time although most people felt
rebuilt filaments were fine. I personally have used rebuilt filaments from
Energy Beam Sciences for over 20 years in a variety of instruments and
cannot tell the difference from brand new manufacturer filaments. I have
no interest in EBS other than a satisfied customer and this opinion is
solely my own and does not necessarily represent my employer.




Norman Elliott | Los Alamos National Laboratory
MST-7 | PO Box 1663
MS E549 | Los Alamos, NM 87545

Phone 505-667-1587
Fax 505-665-2104
e-mail nee-at-lanl.gov





From: oshel-at-ux1.cso.uiuc.edu (philip oshel)
Date: Mon, 25 Nov 1996 09:04:04 -0600
Subject: Re: Rebuilt filaments

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Message-Id: {199611251501.JAA15808-at-ux1.cso.uiuc.edu}
Mime-Version: 1.0
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Just to keep the record straight, I am not a "microscope service
engineer". I am an end user (although I am having to be a "service
engineer"). I grant that not all may have my experience with rebuilt
filaments, but none the less, the remarks below are what I've seen.
Phil

} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} In response to the following:
} ==============================================
} I have to agree that there are problems with rebuilt filaments. I have
} noticed shorter lives and more difficults getting good saturation and
} alignments. The latter two from variations in the mounting of the new
} filament wires on the pegs, and perhaps the solder joints. Rebuilt filaments
} also seem to drift more when new, and near the end of their lives. Phil
} ==============================================
} I would urge caution when painting the performance of all rebuilt filaments
} with the same brush.
...
} In many instances, a microscope service engineer will immediately blame all
} of the microscope's "ills" on the use of retipped filaments, in part because
} he or his firm make a nice commission on the sale of new ones, and this
} could be one reason why there is a bit of a bias against the use of retipped
} filaments.
}



&&&&&&&&&&& Illigitmi non carborundum &&&&&&&&&&&

Philip Oshel
Microscopy
Station A
PO Box 5037
Champaign, IL 61825-5037 USA
(217) 244-3145 days
oshel-at-ux1.cso.uiuc.edu
(217) 355-1143 home

*********** looking for a job again ***********






From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Mon, 25 Nov 1996 11:13:18 -0600
Subject: Re: Problem in JEOL's TEM

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In message {20AE0B31BD0-at-calc.vet.uga.edu} "Buddy Steffens" writes:
} I had a similar problem in a JEOL 100-S several years ago. After
} many service calls, the problem was finally isolated to cooling water
} flow. Our recirculator water had become contaminated and plugged up
} several lines, causing the problem to persist even when the water was
} changed. It finally resulted in much disassembly and cleaning the
} lines out with compressed air. The problem could probably be
} entirely avoided with the use of additives such as antifreeze to the
} coolant, but JEOL service does not like to do this.

I had exatly this problem with my SEM some years ago. Had to use compressed air
to blow out SEM cooling lines, grungy bio-rusty stuff came out, then diffusion
pump stayed on and things were fine. The way to check if this is your problem is
to disconnet your cooling water line at the TEM's OUTPUT and collect water in a
graduated beaker for a specific time, say 10-20 seconds, and calculate the
output flow rate. Compare this with your scope's recommended flow rate. In my
case, it was about a third of what it was supposed to be, got back to normal
after leaning the SEM lines.

Good Luck!

Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"MICROSCOPY & MICROANALYSIS 96" in Minneapolis, Minnesota, Aug.11-15, 1996
Its over, but not forgotten, and it was a blast!





From: psic-at-uclink4.berkeley.edu (Paula Sicurello)
Date: Mon, 25 Nov 1996 10:02:18 -0800 (PST)
Subject: JEOL problems

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http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


We have a JEOL 100CX, a few months ago we also had a problem with the HT
light turning off and losing the beam. It turned out that it was the
pirani gauges that sense the vacuum in the gun and the column. The gauges
needed to be replaced. The gauges thought there were breaks in the vacuum
when none were occurring. Our microscope has 2 of these pirani gauges, one
checks the vacuum in the gun, the other checks the vacuum in the column.
If either on of these is bad the scope will not allow you to get the beam
back. Our service guy found the location of these gauges by checking the
schematics.


Of course first the gun pirani broke and a few months later the column
pirani broke. JEOL does sell these gauges. Oh yeah, JEOL sells the gauges
in packs of 2, one open and one closed. It appears that the open one is
the one that goes bad (at least it did on our machine).

I hope this helps.

Paula = )
U.C. Berkeley
Electron Microscope Lab

"Helping all of UC"






From: ebs-at-ebsciences.com
Date: Mon, 25 Nov 1996 10:36:09 -0600
Subject: Re: rebuilt filaments

Contents Retrieved from Microscopy Listserver Archives
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Hello Wayne & colleagues:

Discoloration of the ceramic base of a tungsten filament, along with
shortened filament life, is usually a very reliable indication of a vacuum leak.

Energy Beam Sciences has manufactured both new and rebuilt tungsten
filaments for electron microscopes for nearly 25 years. In regard to our
own production, I can say with the utmost confidence that there is *no
difference* between new and rebuilt filaments. Filament bases that are
returned to us for rebuilding are cleaned of any contamination, and the same
filament wire and production methods are used to manufacture them. They are
vacuum annealed and centered just like new filaments.

I have often heard comments in the field about differences in performance
between new and rebuilt filaments. Perhaps the filaments in question were
not identical (different wire, different loop configurations) if they were
acquired from different sources. However, rebuilt filaments offer a
tremendous cost savings to the user, and I hate to see this perception of
differences in quality lead users to pay additional money for new filaments
for no good reason.

For this reason, Energy Beam Sciences has always offered a program for new
users of rebuilt filaments: return 10 burned-out filament bases to us, and
we will supply 2 free rebuilt filaments in exchange. We welcome all skeptics!

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: Jim Darley :      p&s-at-ultra.net.au
Date: Tue, 26 Nov 1996 01:22:06 +1100
Subject: Silver targets/sputter coaters

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Message-Id: {1.5.4.32.19961125142206.00676118-at-mailhost.ultra.net.au}
X-Sender: pns-at-mailhost.ultra.net.au
X-Mailer: Windows Eudora Light Version 1.5.4 (32)
Mime-Version: 1.0
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In response to Chuck Garber's "contribution":

Chuck: You forgot the disclaimer. Commercial interest and all that.
Fancy American manufacturing jewellers using fixative sludge and antique
silver for making targets! In Oz they source their raw materials from
precious metal refiners and make items in specified grades.
I had made a good contribution to this particular thread. It is rather poor
to denigrate my item for a little snake oil and cheap advertising. This is
not the purpose of this forum and this is not your first transgression.
Jim Darley
****************************************

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

In response to the following posting:
==============================================
The place to look for silver disks is a manufacturing jeweller wholesaler.
They could be found in Yellow Pages or trade indices. Tell them what you are
after and they make it. Cost of silver is cheap when compared with gold and
the manufacturing charge should not be high either.
I trust the target is not wanted for making mirrors. Large mirrors are made
with silver solutions, but evaporated Al makes much better optical mirrors.
We have a long standing offer to give away some high purity Al wire with
orders.
Cheers
Jim Darley
Probing & Structure (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site: http://www.ultra.net.au/~pns/
===============================================
There are some sound technical reasons why most suppliers of EM supplies and
consumables don't go to jewelry wholesalers:

a) The purity of the silver is not certifiable, and could contain various
alloying elements depending on the (jeweler's) ultimate use of the silver,
and

b) The "fabrication charge" to make small quantities, in that kind of
circumstance, generally erodes any economic savings. Indeed I have been
told in our exhibit booth at meetings that the typical cost to make one this
way costs most persons more than our standard catalog price!

At least this firm would never dream ever of obtaining raw product of such
unknown origin and putting it into commerce and let the unsuspecting
customer be our quality control department!

But the real point is that the market price of a 10 mil thick cathode (most
cathodes from other sources are not that thick ) is only one quarter of the
price of that which was mentioned not long ago on this listserver. In
reality the current price is on the order of $150 depending on current
silver prices. So this hardly sounds like the horrendous price that was
suggested that would motivate one to waste their time seeking out jewelry
firms and to put at risk their important experimental results.

With regard to high purity aluminum wire, the aluminum one gets from
"balling up" ordinary aluminum foil from the super market shelf is more than
adequate for the needs of most persons evaporating Al in an EM lab. Since
one needs quite high purity even to "roll" the aluminum into a foil in the
first place, it is generally thought that if it can be rolled, then it is
more than pure enough for an EM application. I have forgotten exactly what
that purity is generally perceived to be, but if my memory is correct, it is
something like 99.99%!

Chuck
======================================================
Charles A. Garber, Ph. D.
President
SPI SUPPLIES







From: Peter Michiels :      pmi-at-mail2.tornado.be
Date: Mon, 25 Nov 1996 21:13:34 +0100
Subject: "The Beginnings of Electron Microscopy"

Contents Retrieved from Microscopy Listserver Archives
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Hello,

I am currently trying to retrieve a book which is titled "The Beginnings of
Electron Microscopy", edited by Dr Peter W. Hawkes.
I already contacted numerous renowned University book suppliers in both
Belgium and the Netherlands trying to get hold of a copy of Dr Hawkes's
publication.

As none has been able to find a copy I would like to ask this group for
further help.
The full details are:

Hawkes, P.W. (1985), "The Beginnings of Electron Microscopy", Academic
press., Orlando

I sincerely hope that somebody will be able to provide me with any further
information which will enable me to find Dr Hawkes's work.

Yours sincerely,

Ing. Peter Michiels
Philips Electron Optics
Belgium





From: Phil Campbell :      p.campbell-at-qut.edu.au
Date: Tue, 26 Nov 1996 07:34:34 +1000 (EST)
Subject: unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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please unsubscribe me.
****************************************************************************
********
Phil Campbell
Faculty Laboratory Manager
Faculty of Science
Queensland University of Technology

Telephone: (07) 3864 5027
Fax: (07) 3864 5100
email: p.campbell-at-qut.edu.au

****************************************************************************
***************





From: Joe Whelan :      jfwhelan-at-usgs.gov
Date: Mon, 25 Nov 1996 16:06:30 +0000
Subject: laser confocal microscopy

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Message-ID: {3299C406.7300-at-usgs.gov}

I just read an abstract discussing observation of annual growth bands
in speleothem (cave) calcite deposits and one of the tools used to
observe the banding was laser confocal microscopy. Can someone
describe this technology and some of its applications for me?

Thanks




From: Paul.Fischione-at-internetmci.com
Date: Mon, 25 Nov 1996 08:50:54 -0500
Subject: Electropolishing/Plasma Cleaning

Contents Retrieved from Microscopy Listserver Archives
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-- [ From: Paul E. Fischione * EMC.Ver #2.3 ] --

Hello All,

Recently, there has been a great deal of discussion on Electropolishing.
Although electropolishing is very much material and parameter dependent,
once the appropriate conditions have been established, high-quality TEM
specimens can be readily produced. It is imperative to determine the
proper conditions when attempting electropolishing of new materials. For
most metals, electropolishing provides excellent results and should be used
as the preparation method of choice.

Although 30% Nitric in water can be used for Al, 33% Nitric in methanol at -
-30 degrees C and 9 volts is preferred. In addition, a perchloric acid
based electrolyte can be used as follows: 10% Perchloric, 90% Ethanol at 0
degrees C and 7 volts or 5% Perchloric, 20% Butoxyethanol, 75% Methanol at -
-30 degrees C, and between 15 and 50 volts (depending on material).
Polishing at low temperatures greatly slows the reaction and provides
superior results. It should be noted that these parameters have been
successfully employed with the Fischione Twin-Jet Electropolisher.

If an oxide layer is present after electropolishing, it may be removed by
dipping the specimen into a solution of 16 grams Chomic Acid, 35 cc
Phosphoric Acid, and 65 cc Distilled water for 5-10 minutes at room
temperature.

To minimize or eliminate the oxide following electropolishing, it is
essential to rapidly remove the specimen from the polishing cell and rinse
it as quickly as possible. The ability of rapid removal is very much
instrument dependent. It is my opinion that the design of the Model 110
Twin-Jet Electropolisher which we have produced since 1966 affords the most
rapid specimen removal. The specimen holding mechanism is simply lifted
from the top surface of the Electropolisher then either rinsed with a
squeeze bottle of methanol or as Dan Schwartz indicated, to rinse
progressively after perforation. The time from termination to rinse can be
accomplished in less than 2 seconds. By rapid removal and rinsing, post
electropolishing specimen etching is eliminated.

Another alternative to remove the oxide is by plasma cleaning. The removal
is dependent on the ion energy impinging on the specimen. It is important
to consider that ion energies are directly related to the ability to remove
the oxide layer. In our Model 1400 Plasma Cleaner, introduced in 1995, the
ability exists to vary the gas pressure in the plasma chamber by adjusting
the flow of the process gas. There is a corresponding ion energy
associated with the differing gas pressures, thus, allowing precise control
over oxide reduction. Another significant advantage of this method is the
ability to maintain the specimen in a good vacuum. The Model 1400 possesses
an oil-free pumping system with a base vacuum of 10 to the -6 torr. Since
the specimen is already contained in the TEM specimen holder and both the
specimen and the specimen holder are simultaneously cleaned, a rapid
venting of the Plasma Cleaner chamber allows for fast insertion into the
TEM.

Should anyone wish to further discuss any of these issues, please e-mail me
directly or if you will be in attendance at MRS in Boston, I will be
exhibiting in the University Hall of the Marriott, Booth 428.

Thanks and regards,

Paul

Paul E. Fischione, President
E.A. Fischione Instruments, Inc.
9003 Corporate Circle
Export, PA 15632
Phone 412-325-5444
FAX 412-325-5443
e-mail: paul.fischione-at-internetmci.com






From: IAN HALLETT :      ihallett-at-MARCCRI.MARC.CRI.NZ
Date: Tue, 26 Nov 1996 10:01:58 GMT+1200
Subject: Isothermal Freeze-Fixation

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A number of years ago I was involved at looking at ice crystal damage
to fish tissue stored at -20C using isothermal freeze-fixation to
minimise changes to the ice within the tissue. I have now been asked
to look at fish stored at -30C. Does anyone know if it is posible to
do isothermal freeze fixation at that temperature? and has reference
to a formulation? The lowest I have on file is -21C.

Other alternatives would be to freeze substitute at -30C or lower
the temperature and use conventional freeze substitution or freeze
drying. However I am concerned at the changes to ice that might
occur.

Thanks in Advance

Ian


Ian Hallett
HortResearch
Mt Albert Research Centre
Private Bag 92 169
Auckland, New Zealand
Fax 64-9-815 4201
Telephone 64-9-849 3660
EMail ihallett-at-hort.cri.nz




From: CLAYJ-at-ix.netcom.com
Date: Mon, 25 Nov 1996 12:02:36 -0800
Subject: Re: Rebuilt filaments

Contents Retrieved from Microscopy Listserver Archives
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Dear Mr. Garber,

No disrespect intended, but I will take public issue with your statement
that a "a microscope service engineer will immediately blame all of the
microscope's "ills" on the use of retipped filaments, in part because
he or his firm make a nice commission on the sale of new ones"

I am a service engineer for Philips. As a courtesy to our contract customers
we provide, at no charge, spare filaments (10 to a box). Yes, there is a
sales commision that can be made, but OUR cost in comparison is minimal to
the rewards of providing a value-added perk in hopes of keeping our customer
operating uninterrupted. When issues involving short filament life arise, we
examine ALL possibilities and causes. Egg on our face if we said "poorly re-
tipped filaments. Here, try 'ours'", and then have ours fail just as badly.

If you are receiving poor service support or advice, it is wise to resolve
those particular issues with their source, not in this forum.

Respectfully,
Clay Jordan
Customer Service Engineer
Philips Electron Optics
clay_jordan-at-pei.philips.com


On 11/24/96 15:50:43 you wrote:
}
} -- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --
}
} In response to the following:
} ==============================================
} I have to agree that there are problems with rebuilt filaments. I
have
} noticed shorter lives and more difficults getting good saturation and
} alignments. The latter two from variations in the mounting of the new
} filament wires on the pegs, and perhaps the solder joints. Rebuilt
filaments
} also seem to drift more when new, and near the end of their lives. Phil
} ==============================================
} I would urge caution when painting the performance of all rebuilt filaments
} with the same brush. Appreciable numbers of customers worldwide regularly
} use rebuilt filaments, all enjoying considerable cost savings relative to
} the cost of new filaments. Now it is a fact that all rebuilt filaments do
} not all come from the same place, and might not all be created equal, but
} like with anything else, you have to determine which ones work best for
you.
}
} In many instances, a microscope service engineer will immediately blame all
} of the microscope's "ills" on the use of retipped filaments, in part
because
} he or his firm make a nice commission on the sale of new ones, and this
} could be one reason why there is a bit of a bias against the use of
retipped
} filaments.
}
} SPI Supplies offers a filament rebuilding service and we have an obvious
} interest in encouraging the use of rebuilt filaments!
}
} Chuck
}
} ======================================================
} Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
} President 1-(800)-2424-SPI
} SPI SUPPLIES FAX: 1-(610)-436-5755
} PO BOX 656 e-mail: cgarber-at-2spi.com
} West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com
}
}
} Look for us!
} ############################
} WWW: http://www.2spi.com
} ############################
}
}







From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Mon, 25 Nov 1996 19:38:47 -0500
Subject: "Commericals" on the ListServer & Subscribe/Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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Message-Id: {v03007802aebfce0c9525-at-[206.69.208.21]}
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Instructions

Colleagues:

In recent days there has been a rash of postings on the
listserver which are beginning to cross the grey area
between answering a question posted by a individual
and advertising/commericals. While I do not under any circumstances
wish to discourage ANYONE from contributing to the discussion,
however, I find it disturbing when a manufacturer uses this
forum to present their product(s) beyond the ground rules of the server,
or uses the server to critque other manufacturers. (i.e. recent comments
on Rebuilt Filaments, Sputter Coater Targets, Electropolishing/Plasma
Cleaners....)

Allow me to repost the rules of the discussion forum on advertising,
and/or commerical postings and once again ask that you all honor
the ground rules and that arguments be conducted off-line and
not broadcast on the server.

I will also take this opportunity to remind people how to
subscribe/unsubscribe!



Thanks...

Nestor
Your Friendly Neighborhood SysOp

=========================================================

Can I post an Advertisement?
----------------------------

No, that does not fit within the bounds of this discussion forum.

This listserver is not intended to be a Sales mechanism
for commerical organizations, but rather it is an open discussion area
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=======================


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as messages where bouncing all over the world. The current
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however, nothing is impossible and I want to make sure it doesn't
reoccur.






From: Mike Witcomb :      MIKEW-at-gecko.biol.wits.ac.za
Date: Tue, 26 Nov 1996 08:43:57 GMT+2
Subject: Details-Beginnings of EM book

Contents Retrieved from Microscopy Listserver Archives
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Peter Michiels asked for details of a book edited by Peter Hawkes
entitled "The beginnings of electron microscopy". As follows:

Advances in imaging and electron physics. The growth of electron
microscopy. Volume 96. Academic Press. Ed Tom Mulvey. Editor-in-
Chief Peter W Hawkes. Ass. Eds. Benjamin Kazan and Tom Mulvey.
1996. Int Standard Serial No: 1076-5670. Int Standard Book No: 0-12-
014738-6.

Disclaimer: No financial interest, just contributed to it.


Michael J Witcomb PhD
Electron Microscope Unit
University of the Witwatersrand
Private Bag 3
WITS
2050
South Africa

Telephone: + 27 11 716 4000
+ 27 11 716 2419 (messages)
Fax: + 27 11 339 3407
E-mail: mikew-at-gecko.biol.wits.ac.za





From: L. J. Chen :      ljchen-at-faculty.nthu.edu.tw
Date: Tue, 26 Nov 1996 15:00:33 +0800
Subject: TEM - Need help on the career of S. Kikuchi

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Message-ID: {329A9591.7DC2-at-mse.nthu.edu.tw}

S. Kikuchi is reknown for Kikuchi line, Kikuchi map etc. His pioneering
paper on Kikuchi line appeared in Jpn. J. Phys. in 1928. However, little
is known about his career. Does anyone know anything about him except
that he wrote the first paper on Kikuchi line?




From: robert weber :      weber-at-fz-rossendorf.de
Date: Tue, 26 Nov 1996 15:04:27 +0100
Subject: unsubscribe

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Please unsubscribe me from your list.





From: Goran Drazic :      goran.drazic-at-ijs.si
Date: Tue, 26 Nov 1996 15:30:21 +0100
Subject: Asbestos: tremolite/chrysotile ratio determination

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Dear Microscopists,

I am looking for a (practical) method or procedure for the determination
of tremolite/ chrysotile ratio (volume, mass, number of fibers) in bulk
samples (asbestos-cement products). I will be very grateful if you are
willing to share any experience regarding this matter using optical M,
SEM, TEM, XRD or classical analytical chemistry.

Best regards,


Dr. Goran Drazic
Ceramics department
J. Stefan Institute, University of Ljubljana
Slovenia





From: perera-at-ksu.edu
Date: Tue, 26 Nov 1996 10:09:35 -0600 (CST)
Subject: Unsubscribe

Contents Retrieved from Microscopy Listserver Archives
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Unsubscribe




From: Bill Chissoe :      wchiss-at-ou.edu
Date: Tue, 26 Nov 1996 10:05:01 -0600
Subject: Cleaning LaB6

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Message-ID: {329B152D.1EDE-at-ou.edu}

Many thanks to all who responded to my inquiry about cleaning LaB6
wehnelts. There were several good suggestions, all of which sound
better than the method I have been using. Here is a brief summary:
nitric acid on a foam type swab, dilute(1:4) HCl- soak for a minute or
short sequential exposures, a commercial product called "Soap Scum
Remover", soaking in ammonia (1 hr. to overnight), and .25um diamond
paste in water-soluble base. I knew there had to be an easier way!!!
Fortunately, this is not something that needs to be done very often, but
I look forward to trying some of these suggestions in the future.

Thanks,
Bill
--
=============================================================
Bill Chissoe III
Electron Microscopist,University of Oklahoma
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================




From: Fermin, Cesar :      fermin-at-TMC.Tulane.Edu
Date: 26 Nov 1996 12:38:56 -0500
Subject: Histo/EM tech Train

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Message-ID: {n1363110283.9467-at-msmail.tmc.tulane.edu}

I would like information on any program to train combined hitology/EM or
histology alone technicians in the US. I could not find much by calling a few
places. If any training program exist and list server members have information
on the curriculum I would be grateful for information. Thanks.

________ ___________
/ ______/ / _________/ Cesar Danilo Fermin, Ph.D.
/ / / / Professor of Pathology & Otolaryngology
/ / / /_____
/ \______ / ______/ Fax 504 587-7389 & Voice 584-2521
/________/ / / Internet: fermin-at-tmc.tulane.edu
/_______ \/_/
| | | | http://www1.omi.tulane.edu/departments/
| | | | pathology/fermin/cdftop.html
| |______/ |
|________ / Disclaimer: Whatever... is not Tulane's opinion!




From: tom moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Tue, 26 Nov 1996 08:32:03 -0600 (CST)
Subject: Re: laser confocal microscopy

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On Mon, 25 Nov 1996, Joe Whelan wrote:

} I just read an abstract discussing observation of annual growth bands
} in speleothem (cave) calcite deposits and one of the tools used to
} observe the banding was laser confocal microscopy. Can someone
} describe this technology and some of its applications for me?
}
} Thanks
}

As far as I know we were the first group to do this (Dr Luis Gonzalez,
Geology.) It is a very straight-forward technique--polish the sample, put
it on the stage and image with the 488 nm line (usually full power). The
images can then be montaged together and the banding patterns measured.


Thomas Moninger (moninger-at-emiris.iaf.uiowa.edu)
Central Microscopy Research Facility http://www.uiowa.edu/~cemrf
85 EMRB University of Iowa
Iowa City, IA 52242 319-335-8142 FAX 319-335-8049





From: gllovel-at-ppco.com (Gary Lovell)
Date: Tue, 26 Nov 1996 14:22:13 -0600
Subject: Re: laser confocal microscopy

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Message-Id: {199611262022.AA02021-at-relay.ppco.com}

I am thinking of purchasing NORAN'S Electron Flight Simulator 2.0 for
windows. If anyone is familar with this monte carlo modeling software I
would appreciate any information about 1: input parameters required to make
the calculations, 2: any limitations of the software, etc. If any other
company is offering monte carlo modeling software that will run under
Windows95 format , I would be intersed the information about it also.

Thanks in
advance





From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Tue, 26 Nov 1996 08:25:02 -0500
Subject: New virus hoax

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Message-Id: {v03007804aec09fb5d9d5-at-[206.69.208.21]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Another virus hoax. Please do not pass information on it to
the listserver.

Nestor

-------------
} References:
} http://ciac.llnl.gov/ciac/bulletins/h-05.shtml (DoE)



H-05 Internet Hoaxes: PKZ300, Irina, Good Times, Deeyenda, Ghost

November 20, 1996 16:00 GMT


PROBLEM: This bulletin addresses the following hoaxes and erroneous
warnings: PKZ300 Warning, Irina, Good Times, Deeyenda,
and
Ghost.exe
PLATFORM: All, via e-mail
DAMAGE: Time lost reading and responding to the messages
SOLUTION: Pass unvalidated warnings only to your computer security
department or incident response team. See below on
how to
recognize validated and unvalidated warnings and
hoaxes.

VULNERABILITY New hoaxes and warnings have appeared on the Internet and old
ASSESSMENT: hoaxes are still being cirulated.



I} Date: Tue, 26 Nov 96 14:40:58 +0100
} X-Sender: deschuyt-at-rxu01
} Mime-Version: 1.0
} To: ZALUZEC-at-sparc5.microscopy.com
} From: Michel Deschuyteneer {deschuyt-at-sbbio.be}
} Subject: New virus hoax
}
} Dear Nestor:
}
} Two friends in the US have sent me today a "Deeyenda" virus warning .
} As I suspected, it turns out to be of the same breed as the infamous "Good
} Times".
} References:
} http://ciac.llnl.gov/ciac/bulletins/h-05.shtml (DoE)
} http://www.kumite.com/myths/myth027.htm
}
} May I suggest a preemptive note to Microscopy before the list gets cluttered
} with the usual round of messages?
}
} Best regards,
} Michel
} ****************************************************
} Michel Deschuyteneer, Ph.D. deschuyt-at-sbbio.be






From: winey-at-lrsm.upenn.edu (Karen I. Winey)
Date: Wed, 16 Oct 1996 12:08:27 -0500
Subject: Postdoctoral Position

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Message-Id: {n1363082307.33247-at-ematserv.ruca.ua.ac.be}







From: manuela :      manuelap-at-petermac.unimelb.edu.au
Date: Wed, 27 Nov 1996 09:10:00 +1100
Subject: Yeast cells

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Thanks to all of you who replied to my request on the processing of yeast
cells. I now have lots of reading and a variety of methods to try out.

regards

Manuela





From: datye-at-UNM.EDU (Abhaya Datye)
Date: Tue, 26 Nov 1996 16:11:41 -0700
Subject: Job Posting Announcement

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Please refer to the URL at http://www.unm.edu/~cmem

for a job opening at the University of New Mexico

Text version of the posting announcement is appended below:

THE UNIVERSITY OF NEW MEXICO, USA
CENTER FOR MICRO-ENGINEERED MATERIALS
ALBUQUERQUE, NEW MEXICO 87131
VACANCY ANNOUNCEMENT

POSITION: RESEARCH SCIENTIST III
REQUISITION NUMBER: 963185*A
APPLICATIONPERIOD: 11-18-96 to 12-20-96
EEOC JOB GROUP: 3
STARTING SALARY: $3,038.50/MO to $4,179.08/MO
(Dependent on Budget/Policy)
GRADE: 16, Full-Time Position/Regular
PARTIALLY GRANT FUNDED POSITION

Applications from senior level scientists or engineers are invited for the
staff posi-tion of Research Scientist III in the Center for
Micro-Engineered Materials, a Na-tional Science Foundation/University of
New Mexico Industry/University Coop-erative Research Center. The successful
candidate will operate and manage a sur-face and powder characterization
facility. This facility contains a scanning Auger electron spectroscopy and
a scanning electron microscope. It also contains a powder characterization
laboratory that provides thermogravimetric analysis, dif-ferential thermal
analysis, BET surface area analysis, pycnometry, dilatometry and particle
size distribution measurements based on sedimentation velocity. The
suc-cessful candidate will also carry out a successful materials research
program in conjunction with other Center researchers.

The candidate must possess a Ph.D. in chemistry, physics or one of the
engi-neering disciplines or Master's degree in chemistry, physics or one of
the engi-neering disciplines and 4 years of directly related experience or
Bachelor's degree in chemistry, physics or one of the engineering
disciplines and 5 years directly re-lated experience. Directly related
education and experience may be substituted for each other on a year for
year basis.

Desired experience includes extensive hands-on experience with: the
operation and data analysis of Auger electron and X-ray photoelectron
spectroscopy systems; operation of scanning electron microscopes; design
and operation of ultra-high vacuum equipment; computer interfacing to
laboratory instruments; operation of MAC- and PC-based computers for data
analysis. Teaching experience including conducting training sessions for
science and engineering students; demonstrated ability to conduct a high
quality university research program; experience writing proposals for
external funding and a portfolio of research results including techni-cal
publications in refereed journals.

Specific responsibilities of the position include, but are not limited to
operating and maintaining a scanning auger electron spectrometer and
scanning electron micro-scope facility, maintaining various powder
characterization instruments, training students and faculty in the
operation of surface analysis and powder characteriza-tion equipment,
writing proposals to funding agencies and participating in the re-search
programs of a variety of groups associated with the Center.

Applicants must be legally authorized to work in the USA Successful
candidate must be prepared to present degrees, certifications, licenses
etc. to be photocopied and added to the personnel file.

TO APPLY: Applications/resumes, a detailed statement of experience and
three confidential letters of recommendation should be sent to Abhaya K.
Datye, Chair-man of the Search committee, Research Scientist III, Center
for Micro-Engineered Materials, University of New Mexico, Albuquerque, NM
87131 no later than 12/20//96. Applications must include requisition number
and applicant's signature. For further information, please contact William
J. Kroenke, (505)277-6824, Fax (505)277-1024, or Email: yonder-at-unm.edu.






From: jjha-at-202.30.60.10 (Jong -Jae Ha)
Date: Wed, 27 Nov 1996 11:38:25 +0900
Subject: subscribe

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subscribe
jjha-at-202.30.60.10






From: Jong-Jae Ha :      jjha-at-202.30.60.10
Date: Wed, 27 Nov 1996 11:47:08 +0000
Subject: subscribe

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Message-Id: {329C2A3C.4AB-at-202.30.60.10}

Subscribe
(jjha-at-202.30.60.10)




From: bozzola-at-siu.edu (John J. Bozzola)
Date: Tue, 26 Nov 1996 22:29:12 -0600 (CST)
Subject: Service Centers & Finances

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As finances become tighter at universities, administrators are increasingly
adopting "responsibility center management" practices along with insisting
on the recovery of more operating costs. Sometimes this works-but sometimes
it does not, depending on the university.

Does anyone have any experience with the "responsibility center management"
system? What are the specific problems and/or benefits? I will collate the
info and provide it to interested parties. Anonymity will be preserved for
all respondees.

Thanks.

John Bozzola
71 Concordia Drive
Carbondale, IL 62901






From: m.dickson-at-unsw.edu.au (Dr Mel Dickson)
Date: Wed, 27 Nov 1996 13:58:38 +1100 (EST)
Subject: Re: AC field presence detection and cancellation

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} Hello All,
}
} We have a Philips XL40 SEM where it is very hard to focus above
} 20,000 X. I see a wavy pattern as I do slow scans. Is there a
} simple way to test whether this is due to AC fields

See if the effect is worse at long working distances. If it is, that
suggests electrical interference rather than mechanical vibration. Test for
mechanical vibration by putting a dish of water on the microscope and
looking at the reflection of a light in the surface. Ripples = vibration.


There is a neat portable AC field detector called a Gauss Maus ( invented in
Australia!) which you can buy e.g from SPI (Cat #11065-AB, page 72) or
others (no commercial connection, I just have one). You can use it to
measure the RMS strength of the ac fields in 3 axes and to find the source.

You can also get a circular coil of say 1000 turns of enameled copper wire
and display the induced voltage on a oscilloscope. Of course you have no
calibration but it will show you weaker and stronger.

Measures to counter AC magnetic interference:

Oxford Instruments sell a magnetic field cancelling system. USA phone is
(508) 369-9933.

Once you find a source it is possible to move it away from the column.
Sources can include lights (esp fluorescent), metal pipes, air conditioning
ducts, steel reinforcing, power cables and so on. Metal pipes and ducts can
be isolated by putting non conducting plastic segments in the pipe run.

You will also find in the yellow pages, companies that sell magnetically
shielded rooms to exclude radio frequency radiation.

Mel Dickson







From: VISITORS :      visitors_-at-ruca.ua.ac.be
Date: 27 Nov 1996 10:23:32 +0100
Subject: FWD>Postdoctoral Position

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Message-Id: {n1363031838.67244-at-ematserv.ruca.ua.ac.be}
"Microscopy-at-Sparc5.Microscopy.Co" {Microscopy-at-Sparc5.Microscopy.Com}
Cc: winey-at-lrsm.upenn.edu
X-Mailer: Mail*Link SMTP-QM 3.0.1




From: winey-at-lrsm.upenn.edu (Karen I. Winey)
Date: Wed, 16 Oct 1996 12:08:27 -0500
Subject: Postdoctoral Position

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Post Doctoral Position

Department of Materials Science and Engineering &
Laboratory for Research on the Structure of Matter
3231 Walnut Street
University of Pennsylvania
Philadelphia, PA 19104-6272

Qualified candidates will have a Ph.D. in a physical science or engineering
field as well as considerable experience with transmission electron
microscopy. Preferred candidates will have experience with beam sensitive
materials, EELS, and/or polymer microscopy. The successful candidate will
initiate two or more new projects associated with (1) lateral uniformity at
polymer interfaces, (2) electrically active polymeric devices, and/or (3)
optically active polymers. These projects will involve the (soon to arrive)
field emission analytical TEM.


Start Date is flexible.
Earliest start date: December 1, 1996
Latest start date: April 1, 1996


To apply please send the following items to the address above:
1. complete curriculum vitae
2. names and addresses of two references
3. one preprints/reprints


Applications are due November 20, 1996.

regards,
Prof. Karen I. Winey
winey-at-lrsm.upenn.edu




-----------------------------------------------------------------------------
Karen I. Winey
Assistant Professor (215) 898-0593
Materials Science and Engineering Department (215) 573-2128 FAX
3231 Walnut Street winey-at-lrsm.upenn.edu
University of Pennsylvania LRSM building; room 308
Philadelphia, PA 19104-6272



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From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Wed, 27 Nov 1996 09:01:50 +0000
Subject: Re: AC field presence detection and cancellation

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} Hello All,
}
} We have a Philips XL40 SEM where it is very hard to focus above
} 20,000 X. I see a wavy pattern as I do slow scans. Is there a
} simple way to test whether this is due to AC fields and are
} there measures to counter it? Any help is appreciated.
}
}
} Mohan Kalyanaraman
} Mobil Technology Company
} PO Box 480
} Paulsboro, NJ 08066
} 609-224-3989 (ph#)
} mxkalyan-at-pau.mobil.com

First thing to do is confirm that it does originate from the mains supply.
Check your line times for the slow scan and by counting the numbers of
waves across the image you should be able to calculate the frequency of the
intereference. If it is the same (or very close) to the mains frequency, or
an obvious small multiple then its a good bet that the source of the
problem is the mains.

If it is another frequency, then you have to look for other sources which
are likely to be mechanical. This can be simple floor vibrations, or more
complicated things like vibrations on cooling water from pumps with
problems, TMPs with bearing problems, etc. Are there lift shafts nearby,
does anyone operate an ultracentrifuge, etc?

Other electronic sources are possible - I have come across high frequency
interference originating from problems in an HT supply, for example. Also,
poorly shielded computer monitors close to the SEM column can be a source.
Move anything that can be easily moved away from the column.

However, mains is the most probable - having said that, it doesn't need to
be radiated. The best approach is to try to locate and remove the source of
the problem - it MAY be the power lines in or near the SEM but, for
example, incorrectly wired equipment elsewhere in the building, perhaps a
long way away can transmit down the mains and cause problems.

One approach it to try to track the interference as a function of time. Is
it always present, or does it dissappear about 6.00pm (when people quit
work) and reappear about 8.00am when people come back into work? Also, a
many-turns coil of wire connected to an oscilloscope is a sensitive
detector that can help find a source.

My first assumption in these situations is a ground loop - that is the SEM
has accessory equipment connected to it and the SEM and accessory equipment
are connected to the mains (and grounded) at different points, AND grounded
to each other. This can cause all sorts of horrible problems - the usual
approach to solving this is to disconnect everything, apart from the main
SEM - at which point the problem hopefully will dissappear. Then
sequentially reconnect, until the problem reappears. Finally, having found
the offending accessory, make sure it is grounded to the SEM and nowhere
else. Preferably, power it from an accessory power point on the SEM.

But, you might discover that the problem really is AC fields radiated from
mains cabling. In this case, there are commercially available field
cancelling systems. The latest systems seem to work pretty well, although I
have no personal experience.

Regards,

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
---------------------------------------------------------------






From: ldm2-at-apollo.numis.nwu.edu (L.D.Marks)
Date: Wed, 27 Nov 1996 07:36:59 -0600
Subject: PEELS Interface(s)

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We have a model 666 (Gatan) PEELS, one of the
very first before Gatan had a Mac interface. It is
connected to an old workstation (Apollo) which is now
worth far more as tradein than anything else - about
the same speed as a 486 at best. I would be interested
in changing the computer interface to a PC (486 or Pentium).
Are there any interfaces out there?

Laurie Marks




From: manuela
Date: 27 November 1996 01:45
Subject: Yeast cells

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Microscopy-at-Sparc5.Microscopy.Com (MICROSCOPY MSA list messages)
Message-ID: {1996Nov27.140920.1814.199303-at-missgate.sunderland.ac.uk}
X-Mailer: Microsoft Mail via PostalUnion/SMTP for Windows NT
Mime-Version: 1.0
Content-Type: text/plain; charset="US-ASCII"
Organization: University of Sunderland


Manuela

would there be any chance of summarising the yeast references/replies? This
would certainly be useful to me if not other readers on the list.

thanks
Malcolm Haswell
University of Sunderland
UK
e-mail: es0mhs-at-environment.sunderland.ac.uk

----------

Thanks to all of you who replied to my request on the processing of yeast
cells. I now have lots of reading and a variety of methods to try out.

regards

Manuela






From: John Hunt :      hunt-at-msc.cornell.edu
Date: Wed, 27 Nov 1996 09:58:15 -0500 (EST)
Subject: JEOL cooling

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Here is a procedure for cleaning out cooling water lines on a 4000EX given to me by a JEOL service engineer.
Problem: lenses were overheating and shutting off, even though temp.
in was 56 F and out was 92 F. -at- a flow rate of l liter/min. Heat transfer
was insufficient due to copper corrosion which was inside the cooling baffles.
An indication of this was green particles in the bottom of the chiller or hose.
Analysis of the particles suggested Copper Chloride and Sulfate. It is
believed that this was caused by the use of tap water in the chiller.
Flushing the system only cured it for a month.
The following procedure has cured this for at least one year.
1.Cleaned baffles with a snake to remove most of the corrosion. Run
water through the baffle to help flush out particles. Flush both ways.
2.Circulate a 20% solution of acetic acid through the baffles. Do
each baffle separately for 10 minutes each way.
3.Flush with tap water for 5 minutes.
4.Circulate a solution of baking soda to fully neutralize. Mix 4 oz.
to a gallon of water. 5 minutes each way.
5.Flush again. You should get 5 liter/min. with about 45-50 psi
through each baffle. Objective les baffles should be more and condenser less.
6.Clean out chiller reservoir, water lines, valves and regulator. Give
system a final flush with deionized water. Fill chiller with deionized water
and add 1 gram of benzotriazole per liter of water.
John Hunt





From: Sukwon Kang :      skang-at-asrr.arsusda.gov
Date: Wed, 27 Nov 1996 11:48:29 -0500 (EST)
Subject: microtome and wheat

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Hi, everybody!

Did anybody try to get the slice of wheat by using a microtome?

I want to know:

what kind of rasin was used
how to prepare the sample for microtome
any tips for wheat and microtome

After I slice the wheat, get the shape of wheat and reconstruct the wheat
shape.

Thank you and happy holiday.

Suwkon Kang




From: Daniele Spehner :      daniele.spehner-at-etss.u-strasbg.fr
Date: Wed, 27 Nov 1996 17:42:02 +0100
Subject: PRINTER

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Message-ID: {329C6F5A.3CE6-at-etss.u-strasbg.fr}

Dear All,
I am intending on acquiring a high resolution printer for EM pictures.
I have a choice between XL8650 PS/CMY or XLS 8650/PS/CMYK both from
Kodak or the Codonics NP. Do any of you recommend one of these printers
?. I am working on a PC computer under Windows 95.
Thanks,

Danièle SPEHNER
Etablissement de Transfusion Sanguine
10 Rue Spielmann
67065 STRASBOURG, FRANCE




From: Leah Dobbs :      leadob-at-execpc.com
Date: Wed, 27 Nov 1996 07:36:23 -0000
Subject: Exposure time for diffraction patterns

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Message-Id: {199611271800.NAA24479-at-indy2.indy.net}
To: Microscopy list {microscopy-at-Sparc5.Microscopy.Com}

Hello,
We are currently doing diffraction on our TEM for school. Nobody seems to
know what the standard exposure time is when taking a photo of the
diffraction pattern.
I would appreciate any suggestions.

Thank you in advance
Leah L. Dobbs
Madison Area Technical College




From: wise-at-vaxa.cis.uwosh.edu
Date: Wed, 27 Nov 1996 13:24:21 +0000
Subject: FE-SEM reference

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Doers anyone have a good reference that explains the advancements in
imaging made possible by the development of the FE-SEM? I am writing a
paper that uses this technique to image plant mitochondria and I need a
standard paper on the technique/instrumentation that I can refer the reader
to.

Thanks in advance

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-uwosh.edu






From: Bill Chissoe :      wchiss-at-ou.edu
Date: Wed, 27 Nov 1996 14:08:26 -0600
Subject: Thanks

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Message-ID: {329C9FBA.7235-at-ou.edu}

I noticed that my "thank you" received even more suggested ways to
clean LaB6 wehnelts and I appreciate each response. In my initial
summary of suggestions, I forgot to include the one recommending
sonicating for 15 min. in sonicator cleaning solution. A number of the
suggestions satified my criteria for a good technique (ie. minimum elbow
grease, minimum time, minimum wear on wehnelt).

Thanks again,
Bill
--
=============================================================
Bill Chissoe III
Electron Microscopist,University of Oklahoma
E-mail: wchiss-at-ou.edu Ph. (405)325-4391
=============================================================




From: Peter Michiels :      pmi-at-mail2.tornado.be
Date: Wed, 27 Nov 1996 17:17:50 +0100
Subject: Re: AC field presence detection and cancellation

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} Date: Wed, 27 Nov 1996 08:15:17
} To: "Mohan Kalyanaraman" {mxkalyan-at-pau.mobil.com}
} From: Peter Michiels {pmi-at-mailhost.tornado.be}
} Subject: Re: AC field presence detection and cancellation
}
} Hello Mohan,
}
}
} It is very important that you determine whether your 'disturbance' is
mechanical or electromagnetical.
}
} MECHANICAL DISTURBANCES
}
} By changing the working distance (WD) from let's say 5 mm to 30 mm, while
keeping the magnification constant, the amplitude of the disturbance remains
the same.
} With a special tool you can also mount the specimen directly to the final
lens. When the disturbance vanishes it will definitely be a mechanical
disturbance.
}
} INTERFERENCE FIELDS (magnetical and electrical)
}
} By changing the high tension (decreasing from 30 kV to 1 kV), the
disturbance amplitude will increase according to the following formula:
}
} sqrt(30) x amplitude = 5.5
}
} When increasing the WD from 5 mm to 30 mm the amplitude will increase by a
factor of 20!
}
} A magnetic field is easy to simulate by holding a soldering iron or a
transformer near the column. This will result in a 50/60 Hz disturbance.
}
} SOME OTHER TIPS
}
} A dust particle on an aperture can act like an electrical disturbance with
an unstable frequency. Different spotsizes can change the effect.
}
} A pure 50 Hz (or 60 Hz) field or disturbance (flags) can be synchronised by
the mainslock. This is automaticaly done when you select a line time longer
than 20 ms (16.6 ms). When in mainlock you will get some image distortions
rather then flags.
}
}
} That's about it for now. If I come up with some other useful tips, I will
contact you. For now I hope that you can determine exactly what is going wrong.
}
} Good luck!
}
}
}
} Ing. Peter Michiels
} Philips Electron Optics
} Belgium
}
}
}
} At 11:48 26/11/96 EST, you wrote:
} } Hello All,
} }
} } We have a Philips XL40 SEM where it is very hard to focus above
} } 20,000 X. I see a wavy pattern as I do slow scans. Is there a
} } simple way to test whether this is due to AC fields and are
} } there measures to counter it? Any help is appreciated.
} }
} }
} } Mohan Kalyanaraman
} } Mobil Technology Company
} } PO Box 480
} } Paulsboro, NJ 08066
} } 609-224-3989 (ph#)
} } mxkalyan-at-pau.mobil.com
} }
} }
}





From: Peter Michiels :      pmi-at-mail2.tornado.be
Date: Wed, 27 Nov 1996 17:17:50 +0100
Subject: Re: AC field presence detection and cancellation

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} Date: Wed, 27 Nov 1996 08:15:17
} To: "Mohan Kalyanaraman" {mxkalyan-at-pau.mobil.com}
} From: Peter Michiels {pmi-at-mailhost.tornado.be}
} Subject: Re: AC field presence detection and cancellation
}
} Hello Mohan,
}
}
} It is very important that you determine whether your 'disturbance' is
mechanical or electromagnetical.
}
} MECHANICAL DISTURBANCES
}
} By changing the working distance (WD) from let's say 5 mm to 30 mm, while
keeping the magnification constant, the amplitude of the disturbance remains
the same.
} With a special tool you can also mount the specimen directly to the final
lens. When the disturbance vanishes it will definitely be a mechanical
disturbance.
}
} INTERFERENCE FIELDS (magnetical and electrical)
}
} By changing the high tension (decreasing from 30 kV to 1 kV), the
disturbance amplitude will increase according to the following formula:
}
} sqrt(30) x amplitude = 5.5
}
} When increasing the WD from 5 mm to 30 mm the amplitude will increase by a
factor of 20!
}
} A magnetic field is easy to simulate by holding a soldering iron or a
transformer near the column. This will result in a 50/60 Hz disturbance.
}
} SOME OTHER TIPS
}
} A dust particle on an aperture can act like an electrical disturbance with
an unstable frequency. Different spotsizes can change the effect.
}
} A pure 50 Hz (or 60 Hz) field or disturbance (flags) can be synchronised by
the mainslock. This is automaticaly done when you select a line time longer
than 20 ms (16.6 ms). When in mainlock you will get some image distortions
rather then flags.
}
}
} That's about it for now. If I come up with some other useful tips, I will
contact you. For now I hope that you can determine exactly what is going wrong.
}
} Good luck!
}
}
}
} Ing. Peter Michiels
} Philips Electron Optics
} Belgium
}
}
}
} At 11:48 26/11/96 EST, you wrote:
} } Hello All,
} }
} } We have a Philips XL40 SEM where it is very hard to focus above
} } 20,000 X. I see a wavy pattern as I do slow scans. Is there a
} } simple way to test whether this is due to AC fields and are
} } there measures to counter it? Any help is appreciated.
} }
} }
} } Mohan Kalyanaraman
} } Mobil Technology Company
} } PO Box 480
} } Paulsboro, NJ 08066
} } 609-224-3989 (ph#)
} } mxkalyan-at-pau.mobil.com
} }
} }
}





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 27 Nov 1996 15:30:33 -0400
Subject: Re: MagFields

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Message-ID: {n1363013686.43846-at-mse.engin.umich.edu}

Subject: Time: 2:57 PM
OFFICE MEMO RE} MagFields Date: 11/27/96

I never cease to be amazed at how frequently problems with magnetic fields
arise for those of us working with EMs. Our department is just now going
through a situation where the B&G Dept. wants to install a 400 Amp buss line
down the center of our building to service some heavy machining equipment to
be installed by another department. The field B (mG) produced at a distance
X (feet) from a single long straight line of this kind carrying a current C
(Amp) would be B = 6.56 C/X. A few calculations will show that the field
generated by the proposed buss line would put every EM within a distance of
several hundred feet out of business - and then there would also be the
effects of probable vibrations from the machines to contend with. We hope
we are going to be able to convince those in power to abandon their current
plans. If not, our SEMs will be useless.

The source of annoying magnetic fields can be even more subtle and unexpected
than this, however. One morning years ago we turned on our SEM and had
horrible double images. We hooked a coil of wire to an oscilloscope and
followed the source to the middle of the wall directly behind the column.
That led us to explore the office next door, whereupon we found that the
occupant had just started using a rather primative type of electric clock
(apparently received as a gift from one of his children). It took several
minutes to convince him that we had a serious problem, and that he was the
cause of it, but in the end the evidence was convincing - unplug the clock
and the double image went away. On another occasion similar effects were
observed when a silicon carbide rod heating element in a furnace across the
hall came loose and shorted to ground, producing prodigious ground loop
currents in the heating ducts and steel frame members within the walls of the
building.

Incidentally, there was a series of articles in Microscopy Today (Don Grimes,
Ed., MicroToday-at-aol.com) recently on magnetic fields, their causes and
cures. It was written by Curt Dunnam of Linear Research Associates,
Trumansburg, NY 14886 (Fax: 770-368-8256). LRA apparently specializes in
diagnosing and dealing with magnetic fields in EM labs. You might get useful
help from them. I know there are other companies that do the same, but I
don't happen to have names and addresses readily available.







From: es0mhs-at-environment.sunderland.ac.uk (HASWELL Malcolm)
Date: Wed, 27 Nov 1996 14:11:21 GMT
Subject: RE: Yeast cells

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Microscopy-at-sparc5.microscopy.com (MICROSCOPY MSA list messages)
Organization: University of Sunderland


Manuela

would there be any chance of summarising the yeast references/replies? This
would certainly be useful to me if not other readers on the list.

thanks
Malcolm Haswell
University of Sunderland
UK
e-mail: es0mhs-at-environment.sunderland.ac.uk

----------
At 08:29 27/11/96 -0500, you wrote:
} I would be interested in reading the responses you received about
} processing yeast. Would you mind forwarding them to me or writing a
} summary ?
}
} Thank you,
}
} Barbara Hartman
} Barbara.Hartman-at-spcorp.com
}
Here is copy of all the replies...
Also I have found the following paper very useful which I dug up on a
literature search: Robin Wright et al. 1989 TEM and immunocytochemical
studies of yeast: analysis of HMG-CoA reductase overproduction by EM.
Methods in Cells Biology, Vol. 31

If anyone else can shed some more light/advice on the subject please do so.

#1
The ONLY good and reliable method for preparation of
any yeast cell for ultrathin section is (high pressure) freezing
or other good freezing method (slam freezing; propane jet freezing),
combined with freeze substitution.
anything else should only be interpreted with great care.
Lit:
Studer et al. 1989 High pressure freezing comes of age.
Scanning microsc. suppl. 3:253-269
Scanning microscopy Interntl.Chicago,AMF O'Hara, USA
Walther et al 1988 Morphological Organization of Glycoprotein
containing cell surface structures in Yeast
J.Ultrastr. and Molec. Structure research 101: 123-136
C.Capellaro et al 1994 Mating type-specific cell-cell recognition
of Saccharomyces cerevisiae: cell wall attachment
and active sites of a- and alpha-agglutinin.
EMBO J. 13: 4737-4744

With kind regards, Reinhard Rachel

- - Reinhard Rachel {Reinhard.Rachel-at-biologie.uni-regensburg.de}
| )| ) Universitaet Regensburg, Lehrstuhl fuer Mikrobiologie
| \| \ D - 93040 Regensburg (Tel.: xx49-941-943-4534)

http://www.biologie.uni-regensburg.de/Mikrobio/Stetter/Gruppen/rachel.html

#2
Hi Manuela. I maintain an archive of the biologically related
material posted to this list. If you will go to the web address listed at
the end of this message you will see a section called "Tips & Tricks" Go to
the TEM section and there is a link to "TEM of Yeast". It has some protocols
in it but more importantly, e-mail addresses of persons who have done this
before. If you cannot access the info let me know and I will get it to you.

} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
GO GATORS
Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 352-392-1295
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "

#3

Yeast are difficult to handle sometimes. I would suggest that you treat the
cell walls either enzymatically or with metaperiodate in order to facilitate
entry and egress of reagents .

You might look at these two papers

,Byers, B.//Goetsch, L.,,,Preparation of Yeast Cells for Thin-Section
Electron Microscopy,,,,,Guide to Yeast Genetics and Molecular
Biology,,,,,,,,,,,1991,,194,,,602-608,,,,,,,,,,,,,,,,,

Anderson, W.H.//Thompson, E.W.//Zwizinski, C.W.,,,A Rapid Method for the
Preparation of Yeast for Immunoelectron Microscopy Using Lowicryl
HM-20,,,,,Journal of Electron Microscopy Technique,,,,,,,,,,,1991,,18,,2,172-175

*******************************************************
G.W. Erdos, Ph.D. Phone:
352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*****Home of the #1 Gators

"Many shall run to and fro, and knowledge shall be increased"
Daniel 12:4

#4
Dear Manuela,
I've processed yeast cells several times. Standard techniques are fine but
fix for a little longer than usual; up to 24 hrs. is o.k., and dehydrate
with acetone rather than ethanol.
The fix I use is 1.25% glut plus 4% paraformaldehyde in PBS with 4% sucrose.
P.S. I usually process as a cell pellet in ependorf tubes. They spin down
really well into a very dense pellet.
Good luck,
Marilyn

Marilyn Henderson
Centre for Electron Microscopy and Microstructure Analysis
The University of Adelaide
South Australia 5005
marilyn-at-cemmsa.adelaide.edu.au (Marilyn Henderson)

#5
I have embedded Saccharomyces cerevisiae for standard EM using EPON 812 and
Immuno using LRWhite. There is a lot of ref and I do not have them with me
right now but may be able to send in a couple of days. However You need to
treat the yeast suspension with b-glucuronidase ( Sigma, Cat# G-2887) and
Zymolyase 100T ( ICN ImmunoBiological,s Cat# 320931). Outer coating of the
yeast is hard to penetrate thus results in poor infiltration. Fix in 3%
glut(some ref claim to fix in 5% ??) and then run up as you would any cell
pellet. If I can find my protocol I shall email them to you as soon as
possible. Goodluck... }
Regards...
:-) :-) :-) :-) :-) :-) :-) :-) :-) :-) :-) :-)
Visit us at http://www.med.upenn.edu/~path/core/EMCMAIN1.htm
Neelima Shah {shahn-at-mail.med.upenn.edu}

#6

Dear Manuela Palatsides,
In our lab, we are using for many years following fixation protocol:

1. Yeast cells are fixed with 2% (w/v) KMnO4 for 20 min at 0 oC.
2. The cells are then embedded into 2% (w/v) Difco Agar.
3. The embedded cells (approx.1 cubic mm cubes) are dehydrated
in the ethanol series (25%, 50%, 75%, 90%, 95% [each for 15min],
100% [20min], 100% [20min]) and 100% acetone [two times for 20
min].

The samples are then embedded in Vestopal W resin. The ultrathin
sections are stained according to Reynolds (1963). [Reynolds E S:
The use of lead citrate at high pH as an electron-opaque stain in
electron microscopy. J.Cell Biol 17:208-212 (1963)]

This approach gives a good preservation of membrane structrures in
the cells. However, the procedure cannot be use for subsequent
immunolocalization of specific antigens. I do not know if this
fixation procedure works well for Spurrs resin. I do not have any
experieces with it.


The best regards
O. Benada
+---------------------------------------------------------------+
Dr. Oldrich Benada
Acad. Sci. CR Phone: +42-2-4752399
Institute of Microbiology Fax: +42-2-4715743
Electron Microscopy Group E-mail: benada-at-biomed.cas.cz
Videnska 1083
CZ - 142 20 Prague 4 - Krc
Czech Republic
+












From: manuela :      manuelap-at-petermac.unimelb.edu.au
Date: Thu, 28 Nov 1996 14:23:57 +1100
Subject: staining yeast cells

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Hi everyone,
I have another question regarding yeast cells. We need to stain the
chromatin of the spombe yeast cells I am processing and it has been brought
to my attention that staining the DNA is difficult! I've ben using standard
uranyl acetate and lead citrate staining procedures and the nucleus appears
to be hetergenous and lighter than the cytoplasm. So again if anyone has had
experience in this field or has a reference please guide me in the right
direction....

Many thanks

Manuela





From: Roderick G. Ford :      Roderick.Ford-at-asu.edu
Date: Wed, 27 Nov 1996 21:50:40 -0700
Subject: Re: Exposure time for diffraction patterns

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Leah Dobbs wrote:
}
} Hello,
} We are currently doing diffraction on our TEM for school. Nobody seems to
} know what the standard exposure time is when taking a photo of the
} diffraction pattern.
} I would appreciate any suggestions.
}
} Thank you in advance
} Leah L. Dobbs
} Madison Area Technical College

The best exposure times come through experience on diffraction patterns.
Often, if you lift the small screen and allow an auto exposure at that
time, the result will be very good. But, of course, this depends on how
much of the diffraction pattern is hitting the small screen. This is how
it is with the JEOL 2000FX.

Hope that helps.
Roderick Ford




From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Thu, 28 Nov 1996 08:34:59 +0000
Subject: Re: Exposure time for diffraction patterns

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} Hello,
} We are currently doing diffraction on our TEM for school. Nobody seems to
} know what the standard exposure time is when taking a photo of the
} diffraction pattern.
} I would appreciate any suggestions.
}
} Thank you in advance
} Leah L. Dobbs
} Madison Area Technical College

The short answer is that there isn't a standard exposure time.

Factors to think about are:

1. There is a 'sensitivity' control for the automatic exposure time
detector. This will normally be set to give appropriately exposed images
for the film and development procedure you use. It MIGHT be possible to
determine a setting which works reasonably well for diffration patterns (if
most of the work is similar, for example SADP of thin specimens) - take a
series of photos of a 'typical' diffraction pattern at different settings
and process. Users can then change the setting as appropriate depending on
whetther they are photographing images or diffraction patterns.

Of course, the problem here is certainly going to be that somebody will
forget to re-position the switch, or check its setting, and end up taking a
cassette of images on the diffraction pattern setting ...... A LARGE notice
might help.

2. The type of electron diffraction pattern and type of specimen will have
a big influence. Traditional SADP from thin specimens results in
diffraction patterns with a huge dynamic range. In this case it is probably
impossible to get all the information in a single negative - a short
exposure will capture the intense spots but a longer exposure will be
needed to get any faint spots. Patterns from thicker specimens and
different types, such as convergent beam, tend to have a smaller dynamic
range.

3. What you want from the pattern will also affect how you photograph it.
You will probably need several images to collect all the information you
need. When I was regularly taking convergent beam patterns, I often took 6
or 8 photos of each pattern - at least 2 different C2 aperture sizes (large
to show holz lines, small for spot positions), 2 or 3 different camera
lengths (very short for holz rings, medium for zero layer detail, and
longer for bright field detail) and 2 or 3 exposure times for each
condition. I used a lot of film :)

4. You may also need to think about how you process the images. It sounds
as though you are using a traditional 'wet' photographic route. One problem
here it that printing paper has less dynamic range in a single exposure
than film. So you can get a negative with lots of information in it, but
then find it impossible to print in a single exposure - this where you have
to go in by hand, with pieces of card (with and without holes). With some
practise, you can expose as many as 3 or 4 different areas of the paper for
different times and end up with a single print that contains all the detail
of the original negative.

To conclude, even with experience, whatever type of diffraction pattern you
are photographing, you'll probably need at least 2 or 3 exposures to be
confident of getting one right.

Regards,

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
---------------------------------------------------------------






From: Hans Kothe :      kothe-at-mwald5.chemie.uni-mainz.de
Date: Thu, 28 Nov 1996 12:04:17 -0800
Subject: Which CCD-camera

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Message-ID: {329DF041.308C-at-mwald5.chemie.uni-mainz.de}

Dear Mister Zaluzec,

We are an electron microscopy group and we search for a new CCDcamera, to
scan our electron diffraction films properly. We got your address from
George Farrants from Calidris. Could you hire us some advice which
CCD-camera is best. We would like to buy an advanced CCD camera with 16
Bit

With best wishes
Hans Kothe
Group Dr. I.G. Voigt-Martin




From: pogany-at-power.szfki.kfki.hu (Pogány Lajos)
Date: Thu, 28 Nov 1996 14:37:49 +0100
Subject: Taylor company

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Hi,
dear colleagues, I would like to know if the Taylor company (for
microanalitical standards) works and if they have an e-mail address or fax
number?
Thanks for your help
Lajos Pogany
pogany-at-power.szfki.kfki.hu


Dr. Lajos Pogany, PhD


Research Institut for Solid State Physics of
the Hungarian Academy of Sciences
XII. Konkoly Thege 29-33 ;
Budapest
Hungary P.O.Box 49
H-1525
phone: 00-36-1-169-9499
fax: 00-36-1-169-5380
e-mail: pogany-at-power.szfki.kfki.hu






From: judy-at-camtwh.eric.on.ca
Date: Thu, 28 Nov 1996 09:05:43 -0500 (EST)
Subject: cryo sections

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We have several boxes of serial cryo sections of mouse eyes, stored with
dessicant at -70deg. We would like to immunostain sections, a few at a time,
from each box. What is the best way to remove a couple slides without damaging
the rest of the frozen ones with freeze-thaw cycles? I have had 2 opinions -
a) do it as quickly as possible so the rest are not thawed. This, however,
has resulted in some ice formation inside the box.
b) let the entire box warm up to room temperature, the dessicant would absorb
any moisture, then refreeze.

Any opinions/experience would be greatly appreciated - these sections are the
result of a LOT of work.

judy


Judy Trogadis
Eye Research Institute of Canada and
University of Toronto
416-603-5088
judy-at-camtwh.eric.on.ca





From: Smith, Peter :      SMithP-at-agresearch.cri.nz
Date: Fri, 29 Nov 1996 08:31 +1200 (NZST)
Subject: ATS coated slides

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Help help!

We are presently having severe problems keeping paraffin embedded sections
attached to our ATS coated slides during in-situ hybridisation processing.
Our fetal tissue is OK, but anything larger has been totally floating or to
the point of being unusable.

We wash our slides well, and then coat them for 10 seconds in a 2% solution
of ATS in acetone. Two washes follow, one in 100% acetone and another in
DEPC water.

Any suggestions would be very gratefully received - this is now becoming a
very major problem in our lab.

Many Thanks
Peter Smith




From: Henrik Kaker :      Henrik.Kaker-at-guest.arnes.si
Date: Thu, 28 Nov 1996 19:36:52 +0100
Subject: Re: Taylor company

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Pog=E1ny Lajos wrote:
} =20
} Hi,
} dear colleagues, I would like to know if the Taylor company (for
} microanalitical standards) works and if they have an e-mail address=
or fax
} number?
} Thanks for your help
} Lajos Pogany
} pogany-at-power.szfki.kfki.hu
} =20
} Dr. Lajos Pogany, PhD
} =20
} =20
} Research Institut for Solid State Physics of
} the Hungarian Academy of Sciences
} XII. Konkoly Thege 29-33 ;
} Budapest
} Hungary P.O.Box 49
} H-1525
} phone: 00-36-1-169-9499
} fax: 00-36-1-169-5380
} e-mail: pogany-at-power.szfki.kfki.hu
} =20

Lajos,

You may found address with phone and fax number in my microscopy vend=
ors
database at:

http://www.kaker.com/mvd/vendors.html

or

http://www2.arnes.si/guest/sgszmera1/vendors.html

Henrik,

--=20
Henrik Kaker
SEM-EDS Laboratory, Metal Ravne d.o.o., Slovenia,=20
Tel: +386 602 21 131 Fax: +386 602 20 436
Henrik.Kaker-at-guest.arnes.si,
http://www2.arnes.si/guest/sgszmera1/index.html
Microscopy Vendors Database:
http://www2.arnes.si/guest/sgszmera1/vendors.html





From: Mathy H :      mathy-at-rdmetal.ulg.ac.be
Date: Fri, 29 Nov 1996 07:56:38 +0100
Subject: Subscribe

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From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Fri, 29 Nov 1996 11:17:48 +0100 (MET)
Subject: Re: MagFields

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About magnetic fields measurments, we have heard of an Australian company
called "the Arlundya division of the Dindima group", but we failed in
finding their present phones and or www address.

They seem to be in Victoria, Australia. Can anyone help?

Thank you in advance,

Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Lluis Sole i Sabaris, 1-3
E-08028 BARCELONA ESPANYA

Tel +34 (9)3 402 16 95
Fax +34 (9)3 402 13 98





From: 101776.3346-at-CompuServe.COM
Date: Fri, 29 Nov 1996 07:39:33 -0500 (EST)
Subject: Microtome and Wheat - reply

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Hello Suwkon,
From our own experience, we can recommend the following:
Concerning the resin: Use a GMA based resin (e.g. HistoResin). The sections are best prepared
with a motorized rotary microtome (e.g. LEICA RM 2155) and a tungsten carbide knife.
Depending on the degree of resin infiltration into the sample, it might be necessary to take the
sections with the scotch-tape method after sectioning. When the resin does only enclose
the sample and does not infiltrate it totally, parts of the wheat may break out
of the block.

I hope, this helps you.

Regards,
Sabine Schöllhammer
Leica Instruments GmbH
Germany




From: Sara Miller :      saram-at-acpub.duke.edu
Date: Fri, 29 Nov 1996 10:08:55 -0500 (EST)
Subject: Re: ATS coated slides

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On Fri, 29 Nov 1996, Smith, Peter wrote:

} Date: Fri, 29 Nov 1996 08:31 +1200 (NZST)
} From: Smith, Peter {SMithP-at-agresearch.cri.nz}
} To: microscopy listserver {Microscopy-at-Sparc5.Microscopy.Com}
} Subject: ATS coated slides
}
}
} Help help!
}
} We are presently having severe problems keeping paraffin embedded sections
} attached to our ATS coated slides during in-situ hybridisation processing.
} Our fetal tissue is OK, but anything larger has been totally floating or to
} the point of being unusable.
}
} We wash our slides well, and then coat them for 10 seconds in a 2% solution
} of ATS in acetone. Two washes follow, one in 100% acetone and another in
} DEPC water.
}
} Any suggestions would be very gratefully received - this is now becoming a
} very major problem in our lab.
}
} Many Thanks
} Peter Smith
}

We use Fisherbrand Superfrost/Plus (Fisher Scientific 800 766 7000) with
good success.


Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Fri, 29 Nov 1996 17:36:44 +0100 (MET)
Subject: lupe for Philips EM301

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Hello all,

We just went into a rather strange problem. Last week our service
engineer dismantled the lupe of our (old) Philips 301 microscope in order
to do his maintenance job... Therefore he put it on a shelf, for further
use...

a few hours later came an unknown guy, found very nice this old object and
decided it would look much better on his living room table than lost on a
laboratory shelf...

Here we are now, how shall we be able to focus the sample, known that
there is no camera on this microscope.

Conclusion: looking for a EM301 lupe, provided it comes from an instrument
that has already no future. We do have a budget for this.


Thank you in advance.

Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Lluis Sole i Sabaris, 1-3
E-08028 BARCELONA ESPANYA

Tel +34 (9)3 402 16 95
Fax +34 (9)3 402 13 98






From: jacobb-at-mh1.lbl.gov (Jacob Bastacky)
Date: Fri, 29 Nov 1996 14:43:52 -0800
Subject: Re: Image Archival

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Message-Id: {v01540b07aec517419e72-at-[131.243.32.51]}
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

Mary Thompson and Lawrence Berkeley Laboratory has developed a nice program
with us that allows Image Archiving with fast text searches and produces
thumbnails of any size you choose. It's available free. Contact her at:

MRThompson-at-lbl.gov

Jacob

Jacob Bastacky
Room 116 Donner Laboratory
Lawrence Berkeley Laboratory EMail: sjbastacky-at-lbl.gov
University of California Phone: (510) 486-4606
Berkeley, California 94720 Fax: (510) 486-4750






From: Lawrence Kordon :      nikon-at-jagunet.com
Date: Fri, 29 Nov 1996 22:06:34 -0500
Subject: Image Database Software

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Message-ID: {329FA4B2.EC8-at-jagunet.com}

List Members,

I am trying to help a local Researcher solve a minor problem. She
captures Digital Photomicrographs and wants to archive these images. It
would be nice if it worked on both Mac and/or Windows, had the ability
to show thumbnails and be customizable from a fields standpoint. I am
very verse with Filemaker Pro by Claris which would cover all these
criteria; however, I do not want to "reinvent the wheel" and feel
someone out there has done this already!

If you know of a template(s) for Filemaker or any other image databases;
shareware or commercial, please direct me to it. Also, since I am a
member of the notorious "Vendor Class" I would like to state up front
this is not going to be used for commercial purposes or monetary gain!
(I am just being a good friend and worthy consultant). Nonetheless, I
am willing to show my gratitude and reward the person generously that
best solves my dilemma.

Thank you,

Lawrence Kordon
Nikon, Inc.
Columbia, Maryland
(410) 740-7404
nikon-at-jagunet.com




From: Tang Ee Koon :      medlab2-at-leonis.nus.sg
Date: Sat, 30 Nov 1996 11:42:26 +0800 (SST)
Subject: carbon coated mica

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Can anyone tell me how to process carbon-coated mica, the heavy salt for
EM? This is a question asked by a friend. Thank you very much.






From: Yves Maniette[SMTP:yves-at-giga.sct.ub.es]
Date: Sat, 30 Nov 1996 21:20:24 +-1100
Subject: Re: MagFields

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Message-ID: {01BBDF06.BF653760-at-slmel12p37.ozemail.com.au}
"'Yves Maniette'"
{yves-at-giga.sct.ub.es}
Cc: Micros/MagFlds {microscopy-at-Sparc5.Microscopy.Com}

Dear Yves,

Just had a look in my local phone book for you...

Arlunya Division of the Dindima Group are located at 10 Argent Place, Ringwood, Victoria, Australia, 3134.
Telephone no. is international +61 3 9873 4455.

If you don't have any luck, let me know and I'll chase up a fax number for you.

Hope this helps, Roger


Roger Wallis
General Manager
Optiscan P/L Confocal Microscopy
PO Box 1066
Mt. Waverley MDC
Victoria 3149 Australia
Tel: (61) 3-9562 7741
Fax: (61) 3-9562-7742
e-mail: rogerw-at-optiscan.com.au
URL: http://www.optiscan.com.au
______________________________


----------


About magnetic fields measurments, we have heard of an Australian company
called "the Arlundya division of the Dindima group", but we failed in
finding their present phones and or www address.

They seem to be in Victoria, Australia. Can anyone help?

Thank you in advance,

Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Lluis Sole i Sabaris, 1-3
E-08028 BARCELONA ESPANYA

Tel +34 (9)3 402 16 95
Fax +34 (9)3 402 13 98








From: Eric Steel :      eric.steel-at-nist.gov
Date: Sat, 30 Nov 1996 17:55:45 -0500
Subject: Post Doc Opportunities

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The National Institute of Standards & Technology has many Post Doctoral
Positions open. These are offered competitively through the National
Research Council. Within microscopy/microanalysis research areas we have
several possible openings described at the following sites:

http://rap.nas.edu/lab/NIST/50837106.html
This opportunity highlights our analytical research using Analytical
Electron Microscopy/Compositional Mapping

http://rap.nas.edu/lab/NIST/50837109.html
This opportunity highlights our Submicroscopic Chemical and Physical
Characterization of Materials and Particles

http://rap.nas.edu/lab/NIST/50837110.html
This opportunity highlights our Electron-Probe
Microanalysis/Scanning Electron Microscopy research.

If you have research ideas that would be related to these analytical
approaches and are looking for a Post Doc opportunity, please contact me
immediately.

The NIST/NRC program offers a two year post doc at an annual salary of
$45,500. The applications are due to the NRC by Jan 15, 1997! This
includes a proposal and several recommendations.

A candidate must be a U.S. citizen that receives their PhD and starts work
at NIST by Feb 1, 1998 (You can start as early as July 1, 1997). So, this
is the perfect opportunity for those of you that are graduating this spring
through next fall. Please note that NIST/NRC only accepts applications once
a year, unlike some other institutions.


Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov
Microanalysis Research Group Office: 301-975-3902
N.I.S.T. FAX: 301-216-1134
Bldg. 222/Rm A113
Gaithersburg MD 20899





From: bethd-at-casbah.acns.nwu.edu (Elizabeth Dickey)
Date: Sun, 1 Dec 1996 05:34:07 -0500
Subject: Unsubscribe

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From: Microscopy-request
Date: Saturday, November 30, 1996 11:42AM
Subject: carbon coated mica

Contents Retrieved from Microscopy Listserver Archives
http://www.microscopylistserver.com/MicroscopyListserver/MicroscopyArchives.html


Can anyone tell me how to process carbon-coated mica, the heavy salt for
EM? This is a question asked by a friend. Thank you very much.






From: Microscopy-request
Date: Wednesday, November 27, 1996 7:36AM
Subject: Exposure time for diffraction patterns

Contents Retrieved from Microscopy Listserver Archives
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Hello,
We are currently doing diffraction on our TEM for school. Nobody seems to
know what the standard exposure time is when taking a photo of the
diffraction pattern.
I would appreciate any suggestions.

Thank you in advance
Leah L. Dobbs
Madison Area Technical College




From: H.BRINKIES -SE108/TEL.8657 :      hbrinkies-at-swin.edu.au
Date: Mon, 2 Dec 1996 17:09:16 EST+11
Subject: Re: MagFields

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Message-Id: {199612020608.AA31275-at-lucy.swin.edu.au}
Comments: Authenticated sender is {hbrinkies-at-gpo.swin.edu.au}

} Date: Fri, 29 Nov 1996 11:17:48 +0100 (MET)
} From: Yves Maniette {yves-at-giga.sct.ub.es}
} To: Wil Bigelow {Wil_Bigelow-at-mse.engin.umich.edu}
} Cc: Micros/MagFlds {microscopy-at-Sparc5.Microscopy.Com}
} Subject: Re: MagFields

}
} About magnetic fields measurments, we have heard of an Australian company
} called "the Arlundya division of the Dindima group", but we failed in
} finding their present phones and or www address.
}
} They seem to be in Victoria, Australia. Can anyone help?
}
} Thank you in advance,
}
} Yves MANIETTE
} Universitat de Barcelona
} Serveis Cientifico Tecnics
} Unitat ESCA TEM
} Carrer Lluis Sole i Sabaris, 1-3
} E-08028 BARCELONA ESPANYA
}
} Tel +34 (9)3 402 16 95
} Fax +34 (9)3 402 13 98
}
Hi Yves.

I looked up the local telephone book and found the following address
and phone number that might be what you are looking for.

THE DINDIMA GROUP P/L
10 Argent Place
RINGWOOD, Victoria, 3134
Australia
Telephone Number +61 3 9873 4455

Regards

Hans Brinkies
SWINBURNE, University of Technology
School of Mechanical and Manufacturing Engineering
Industrial Microscopy
HAWTHORN, Victoria, 3122
Australia




From: Self :      Hans.MM.DSED.Swinburne
Date: Mon, 2 Dec 1996 08:23:04 EST+11
Subject: Re: MagFields

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Message-Id: {199612020608.AA08671-at-lucy.swin.edu.au}
Comments: Authenticated sender is {hbrinkies-at-gpo.swin.edu.au}

Forwarded message:

} Date: Fri, 29 Nov 1996 11:17:48 +0100 (MET)
} From: Yves Maniette {yves-at-giga.sct.ub.es}
} To: Wil Bigelow {Wil_Bigelow-at-mse.engin.umich.edu}
} Cc: Micros/MagFlds {microscopy-at-Sparc5.Microscopy.Com}
} Subject: Re: MagFields

}
} About magnetic fields measurments, we have heard of an Australian company
} called "the Arlundya division of the Dindima group", but we failed in
} finding their present phones and or www address.
}
} They seem to be in Victoria, Australia. Can anyone help?
}
} Thank you in advance,
}
} Yves MANIETTE
} Universitat de Barcelona
} Serveis Cientifico Tecnics
} Unitat ESCA TEM
} Carrer Lluis Sole i Sabaris, 1-3
} E-08028 BARCELONA ESPANYA
}
} Tel +34 (9)3 402 16 95
} Fax +34 (9)3 402 13 98
}
}
Hi Yves.

I looked up the local telephone book and found the following address
and phone number that might be what you are looking for.

THE DINDIMA GROUP P/L
10 Argent Place
RINGWOOG, Victoria, 3134
Australia
Telephone Number +61 3 9873 4455

Regards

Hans Brinkies
SWINBURNE, University of Technology
School of Mechanical and Manufacturing Engineering
Industrial Microscopy
HAWTHORN, Victroria, 3122
Australia





From: timon.fliervoet-at-uni-bayreuth.de (Timon Fliervoet)
Date: Mon, 2 Dec 1996 08:39:54 +0100
Subject: RE: Exposure time for diffraction patterns

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To follow up in the discussion on diffraction pattern exposure time. For
normal diffraction patterns I use, as a general rule of thumb, 1/5th to
1/10th of the time given by the microscope. E.g. If the exposure time given
by the microscope would be 30 seconds, I will expose the film approximately
3 - 5 seconds. This is giving me good results, most diffraction spots can
be seen and the central / transmitted beam is not too bright. Of course if
you need to see more details in the diffraction pattern one needs to
experiment.

Timon

---------------------
Timon Fliervoet
Bayerisches Geoinstitut, Universitat Bayreuth
D-95440, Bayreuth, Deutschland
tel: ++49 921 553745; fax: ++49 921 553769






From: Yves Maniette :      yves-at-giga.sct.ub.es
Date: Mon, 2 Dec 1996 12:20:21 +0100 (MET)
Subject: RE: MagFields+binocular for EM300

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Hello all,

thanks to all of you who have answered my questions. Here is the relevant
information:

1.1. Two addresse are quoted, maybe the first one is more recent:

THE DINDIMA GROUP P/L
10 Argent Place
RINGWOOG, Victoria, 3134
Australia
Telephone Number +61 3 9873 4455

AND/OR

Post Office Box 106
VERMONT,
VIC 3133
AUSTRALIA

PHONE;
+613-9873-4455
FAX:
+613-9873-4749

1.2. Email is: 100241.3642-at-compuserve.com.au

1.3. If you are in USA, better deal with Chuck (cgarber-at-2spi.com), he has
already done for you the custom process and distributes Arlunya products.

1.4. If you are in Pakistan or nearby country then it might be more useful
to get in touch with M. Ashfaq Ali,

Probe Scientific Int'l,
Suite # 9, Ali Aptts,
Block-7, F. B. Area,
P.O. Box 13784,
Karachi-75950,
PAKISTAN Fax: 92-21-6674365, E-mail: psi-at-zrk.khi.erum.com.pk


2. "Lupe" (actually binocular, sorry for the mistake) I have got several
interesting answers and will answer them privately.



Yves MANIETTE
Universitat de Barcelona
Serveis Cientifico Tecnics
Unitat ESCA TEM
Carrer Lluis Sole i Sabaris, 1-3
E-08028 BARCELONA ESPANYA

Tel +34 (9)3 402 16 95
Fax +34 (9)3 402 13 98





From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 2 Dec 1996 13:44:02 -0500 (EST)
Subject: Re: Which CCD-camera

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Alternate-Recipient: allowed
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} We are an electron microscopy group and we search for a new CCDcamera, to
} scan our electron diffraction films properly. We got your address from
} George Farrants from Calidris. Could you hire us some advice which
} CCD-camera is best. We would like to buy an advanced CCD camera with 16
} Bit
}
Dear Hans,
One caution about using a CCD to scan film is that stray light can
be picked up as arising from other pixels. This results in higher light
levels (lower OD) being recorded than actually is there. This is particu-
larly troublesome for the very intense spots. Remember, a spot with OD = 4
has only 1/100 of a % of the incident light transmitted, so very small
amounts of stray light will cause large errors. The most accurate method of
scanning film uses an instrument which has a small beam and a small detec-
tor slit, such as the Perkin-Elmer, Optronics or others with which I am
not familiar. This accuracy for the intense spots may not be necessary
for your application, and using a scanning microdensitometer takes a long
time. Good luck.
Yours,
Bill Tivol




From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 2 Dec 1996 14:16:40 -0500 (EST)
Subject: Re: Exposure time for diffraction patterns

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[snips]
} } We are currently doing diffraction on our TEM for school. Nobody seems to
} } know what the standard exposure time is when taking a photo of the
} } diffraction pattern.
}
} The short answer is that there isn't a standard exposure time.
}
True, the best exposure time depends on the specimen, the use to
which the data will be put and the method used to extract the data from
the film. Obviously, the more intense the pattern, the shorter the ex-
posure. If all that is of interest is measurement of the lattice para-
meters, you want an exposure which gives as many accurate spot positions
as possible. If you want accurate intensity measurements, you may want
to keep the exposure short enough so that the spot OD's are in the linear
range of the film's response. (An alternative is to calibrate the expo-
sures with a blackening curve.) As pointed out by others, this may require
several exposures. If you are measuring the film with a scanning micro-
densitometer, you can have up to OD = 4 and not lose too much accuracy,
but if you use a CCD, you have to lower the exposure to the point where
stray light does not cause errors measuring the more intense spots.

} Factors to think about are:
}
} 2. The type of electron diffraction pattern and type of specimen will have
} a big influence. Traditional SADP from thin specimens results in
} diffraction patterns with a huge dynamic range. In this case it is probably
} impossible to get all the information in a single negative - a short
} exposure will capture the intense spots but a longer exposure will be
} needed to get any faint spots.

Using LoDose film and a Perkin-Elmer 1010A microdensitometer at
a window setting of 10 micron square, I have been able to recover both
the low-order spots (OD up to ~4) and faint spots (not visible to the
eye, but they integrate to a positive value; background and systematically
absent reflections are either negative or within statistics of 0).
}
} 3. What you want from the pattern will also affect how you photograph it.
} You will probably need several images to collect all the information you
} need.

In addition, it can be useful to have a sequence of patterns from
one area so that you can see if the crystal is being degraded in the beam.
If necessary, you can extrapolate the measurements to 0 dose.
}
} 4. You may also need to think about how you process the images. It sounds
} as though you are using a traditional 'wet' photographic route. One problem
} here it that printing paper has less dynamic range in a single exposure
} than film. So you can get a negative with lots of information in it, but
} then find it impossible to print in a single exposure - this where you have
} to go in by hand, with pieces of card (with and without holes). With some
} practise, you can expose as many as 3 or 4 different areas of the paper for
} different times and end up with a single print that contains all the detail
} of the original negative.
}
If you scan the negative into a computer, you needn't worry about
the printing. If you want prints for publication, they can be obtained
from computer files. The advantage here is that the files can be mani-
pulated (by subtracting background, rescaling various areas, etc.) so that
the pertinant info can be readily seen. Of course, all such manipulations
must be described in the paper.

} To conclude, even with experience, whatever type of diffraction pattern you
} are photographing, you'll probably need at least 2 or 3 exposures to be
} confident of getting one right.
}
I am not confident after 2 or 3 exposures, but I *can* get the
right exposure after I see the results of the first 2 or 3. BTW, use
at least a factor of 4 between exposures; i.e., 1, 4 and 16 sec, etc.
Good luck.
Yours,
Bill Tivol




From: Mary Thompson :      mrt-at-george.lbl.gov
Date: Mon, 02 Dec 1996 12:33:07 -0800
Subject: RE: Image Archiving program

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X-Mailer: exmh version 1.6.7 5/3/96
montpetitd-at-em.agr.ca, wamann-at-metalmat.ufrj.br,
george.braybrook-at-ualberta.ca, I.Ivanov-at-ix.netcom.com,
clsmteam-at-imiucca.csi.unimi.it, barbara-at-gatan.com,
K.R.Hallam-at-bristol.ac.uk, shcherbina-at-cbr.med.harvard.edu
cc: microscopy-at-Sparc5.Microscopy.Com


What Jacob is referring to is a software package called ImgLib devoloped at
Lawrence Berkeley Lab to help organize and browse digital images via a Web
browser. The relevant URL is http://imglib.lbl.gov/ImgLib. Check out the
"For documentation or to download software" button for a description of what
it does. Try "Introduction" first. The "Index to all the collections" button
lets you try out the searching/browsing interface. The Berkely Lab collection,
and LungStructure are accessible without a password.

The executive summary is:
Server runs on a Unix system, currently Solaris 2.5 and mostly consists of
Perl scripts called by the HTTP deamon. It could be fairly easily
ported to other Unix systems. It also uses several portable image
processing libraries which would make it harder to port to Windows
or MacOS.
The client side is just a Web browser. It is optimized a bit for Netscape.
The code is downloadable from our Web site. If you actually want to copy it
and get it running on your Unix server, I will probably have to help
you a bit, though there is a README file included in the tar file.

Mary

- ---------------------------------------------------------------------
Mary R. Thompson work - {MRThompson-at-lbl.gov}
Imaging & Distributed Computing Group (510) 486-7408
Lawrence Berkeley National Lab home - thompson-at-dnai.com
(510) 540-6538
- ----------------------------------------------------------------------









From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 2 Dec 1996 15:09:59 -0400
Subject: Error-MagFields

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Message-ID: {n1362577181.55859-at-mse.engin.umich.edu}

Subject: Time: 3:01 PM
OFFICE MEMO Error:MagFields Date: 12/2/96

I apparently gave the wrong FAX number for Linear Research Associates in my
last listserver memo on magnetic fields in EM labs. The following corrective
message, addressed to me, is self explainatory:
- - - - - - - - - - - - - - - - - - -
Dr. Bigelow,

Don Grimes of Microscopy Today has forwarded to my attention your
recent microscopy listserv memo on the interesting topic of ubiquitous,
annoying magnetic fields. In your memo, you refer to my recent article
series in MT on interfering magnetic fields, which I certainly appreciate.
I am writing to mention that the fax number you cite in your memo is
unfortunately not correct. The actual fax number for Linear Research
Associates is 607-387-7806 (v: 607-387-3411). We will of course be glad to
provide further descriptive materials for anyone interested in our systems,
services or research work in the area of active-feedback magnetic field
mitigation.


Best regards,
Curt Dunnam crd4-at-cornell.edu







From: tom moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Tue, 26 Nov 1996 08:32:03 -0600 (CST)
Subject: Re: laser confocal microscopy

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From: Beverly E Maleeff
Date: 2 Dec 96 15:59:05 EDT
Subject: PSM December '96 Meeting Notice

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To: microscopy {microscopy-at-Sparc5.Microscopy.Com}
{Beverly_E_Maleeff-at-sbphrd.com}

PHILADELPHIA SOCIETY FOR MICROSCOPY

DECEMBER 1996 MEETING NOTICE
Thursday, December 12, 1996

Seeing Polymer Ultrastructure

Matthew R. Libera, Sc.D.
Department of Materials Science and Engineering
Stevens Institute of Technology
Hoboken, NJ

Just as they fill an important niche in the study of inorganic engineering
materials, the various techniques
of transmission electron microscopy (TEM) play an important role in the study
of polymers. Traditional
polymer-imaging methods employ heavy-element stains to preferentially label
microstructural features.
These have contributed substantially to the understanding of polymer
structure/property relations, but they
are not without significant shortcomings. This presentation will briefly
review how staining leads to contrast
with examples from homopolymer blends and microphase-separated block
copolymers. Ongoing research
to develop microstructural mapping techniques based on electron energy-loss
spectroscopy will then be discussed.
Examples will be presented where contrast can be generated in blends due to
spatial variations in both valence
(polyethylene/polystyrene) and core (polyethylene/nylon) electron structure.
The presentation will conclude
with a brief description of a second method - transmission electron holography
- which offers an opportunity
for phase-contrast imaging of unstained multiphase polymers.


DATE: Tuesday, December 12, 1996

PLACE: Laboratory for the Research of Science and Materials
(LRSM) Building, 33rd and Walnut Street, Philadelphia, PA.
(On the campus of the University of Pennsylvania)
Parking is available behind the LRSM Building after 5:00 PM.

TIME: 5:30 PM Social hour, hosted by our meeting sponsors

6:30 PM Holiday Dinner
Members $12.00 Student members $6.00 Non-members $15.00

Menu: Beer, wine, assorted soda and bottled water
Munchies

Hickory ham with apricot glaze
served with orange halves and cranberry relish
Tortellini with broccoli and red peppers
Salad of winter greens with dressing
Chef's vegetable du jour
Rolls and butter

Cherry topped cheesecake with fresh mint

Coffee, decaf or tea


7:30 PM Speaker


Reservations will be taken by Ms. Pat Overend at the University of
Pennsylvania, 215/898-8337.
Deadline for reservations will be Tuesday, December 10. If you have any
questions regarding
the meeting please feel free to contact Rollin Lakis of the Executive Council
at 215/898-8718.
Cancellations must be received by Ms. Overend no later than 5:00 PM, December
10, 1996.

RESERVATIONS ARE REQUIRED FOR DINNER. We cannot guarantee you a meal if you
do not make a reservation by the deadline above.





From: mrt-at-george.lbl.gov (Mary Thompson)
Date: 96-12-01 19:52:55 EST
Subject: Fwd: Image Archiving program

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---------------------
Forwarded message:

Could one of you please forward my reply to the list that Jacob mentioned the
ImgLib software on.

What Jacob is referring to is a software package called ImgLib devoloped at
Lawrence Berkeley Lab to help organize and browse digital images via a Web
browser. The relevant URL is http://imglib.lbl.gov/ImgLib. Check out the
"For documentation or to download software" button for a description of what
it does. Try "Introduction" first. The "Index to all the collections" button
lets you try out the searching/browsing interface. The Berkely Lab
collection,
and LungStructure are accessible without a password.

The executive summary is:
Server runs on a Unix system, currently Solaris 2.5 and mostly consists
of
Perl scripts called by the HTTP deamon. It could be fairly easily
ported to other Unix systems. It also uses several portable image
processing libraries which would make it harder to port to Windows
or MacOS.
The client side is just a Web browser. It is optimized a bit for Netscape.
The code is downloadable from our Web site. If you actually want to copy
it
and get it running on your Unix server, I will probably have to help
you a bit, though there is a README file included in the tar file.

Mary

---------------------------------------------------------------------
Mary R. Thompson work - {MRThompson-at-lbl.gov}
Imaging & Distributed Computing Group (510) 486-7408
Lawrence Berkeley National Lab home - thompson-at-dnai.com
(510) 540-6538
----------------------------------------------------------------------








From: Barry, Lilith :      Lilith.Barry-at-nrc.ca
Date: Tue, 3 Dec 1996 08:41:00 -0500
Subject: scanner

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Message-ID: {c=CA%a=_%p=NRC%l=NRC/IMSD/000719CC-at-imsd-exchange.nrc.ca}

That topic again! Sorry, I haven't been keeping the information on
scanners and now I have to make fast decision on buying one. Is there
any good scanner that one can digitize from microscope slides,
negatives, autoradiographic film..? If there is one, we have to be able
to attach it to a PC and Mac. And also the approximate price, if you
know.
Thank you,

Lilith Ohannessian-Barry e-mail: lilith
barry-at-nrc.ca
National Research Council Tel: 613-993-6460
Institute of Biological Sciences Fax: 613-941-4475
Montreal rd Campus, M54
Ottawa, Ont. K1A 0R6
CANADA





From: Dorota Wadowska :      wadowska-at-upei.ca
Date: Tue, 03 Dec 1996 09:47:09 -0400 (AST)
Subject: TEM-protein A gold

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X-PMrqc: 1

Hi there!
Can anyone recommend a commercial source of good quality protein A
gold (20 nm).
Thanks
Dorota




From: Bob Hirche :      bhirche-at-usit.net
Date: Tue, 3 Dec 1996 12:14:34 -0500 (EST)
Subject: Unsubscribe

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"Unsubscribe pls."





From: Dr Eric E. Lachowski :      che136-at-abdn.ac.uk
Date: Tue, 3 Dec 1996 17:19:08 +0000 (GMT)
Subject: diffraction patterns

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In the good old days before electron microscopes had
exposure meters, the generally accepted method of getting
the right exposure for SAD patterns was to overfocus the
illumination until the diffraction spots were only just
visible and expose for about 60 seconds. Obviously,
"just visible" is a highly subjective condition, but
there is considerable latitude with this method and you
can usually get away with just one exposure unless the
crystal is very thick. Weak reflections usually show up
quite well, even ones which are not visible to the eye at
normal brightness. Keeping the illumination well
overfocussed has the additional advantage of ensuring
that the spots are as sharp as possible. Of course the
difficulties with printing the patterns remain, but these
have already been discussed in this forum.

----------------------
Dr Eric E. Lachowski
e.lachowski-at-abdn.ac.uk







From: peggy-at-research.nj.nec.com (Peggy Bisher)
Date: Tue, 3 Dec 1996 13:42:32 -0400
Subject: Aluminium Grids

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Does anyone know where I can purchase aluminium grids for TEM?
I do not want ones that have a mesh, but rather ones with just a
large hole.
The bigger the hole ( {2000=B5m) the better.

Thank you, Peggy






Margaret E. Bisher

NEC Research Institute
4 Independence Way
Princeton, NJ 08540.
Tel.: (609) 951-2629
=46ax: (609) 951-2496
e-mail: peggy-at-research.nj.nec.com








From: Michael J. Lyon :      lyonm-at-vax.cs.hscsyr.edu
Date: Tue, 03 Dec 1996 15:46:44 -0500
Subject: Cryostat Sectioning Bone

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I need some help in obtaining cryostat sections of undecalcified bone. I
am using diffusible tracers and cannot decalcify the bone. Any
suggestions would be welcomed on sectioning angle, temperature, knives,
etc. The piece that I need to section is about 5-7 mm square and I need
to have sections of at least 20 um in thickness.

Thanks in advance

Mike




From: Paul Webster :      paul.webster-at-Yale.edu
Date: 3 Dec 1996 17:09:02 -0500
Subject: Re: TEM-protein A gold

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Message-ID: {n1362489175.90206-at-QuickMail.Yale.edu}

Dorota Wadowska asks
"Can anyone recommend a commercial source of good quality protein A
gold (20 nm)."

We get ours from an interesting but reliable source:

The University of Utrecht,
Department of Cell Biology,
Medical School, AZU HOZ.314
Heidelberglaan 100,
3584 CX Utrecht, The Netherlands.

Fax 011 31 30 541 797.

The contact name is George Postuma but the enterprise is run by Jan Slot (famous
in colloidal gold and immunocytochemical circles).

Colloidal gold coupled to protein has a short shelf life so their deal is for
them to sell you 1 ml of gold probe which they deliver in quarterly
installments. Each instalment is delivered soon after it is made so there are
no storage problems. I think they will even make customised preparations of
colloidal gold.

We have great succes with our probes and get no consideration from our supplier
for recommending them.

Best regards

Paul Webster
Center for Cell Imaging
Yale School of Medicine
Come check us out at
http://info.med.yale.edu/cellimg







From: Sara Miller :      saram-at-acpub.duke.edu
Date: Tue, 3 Dec 1996 16:11:20 -0500 (EST)
Subject: Re: TEM-protein A gold

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On Tue, 3 Dec 1996, Dorota Wadowska wrote:

} Date: Tue, 03 Dec 1996 09:47:09 -0400 (AST)
} From: Dorota Wadowska {wadowska-at-upei.ca}
} To: microscopy-at-Sparc5.Microscopy.Com
} Subject: TEM-protein A gold
}
} Hi there!
} Can anyone recommend a commercial source of good quality protein A
} gold (20 nm).
} Thanks
} Dorota
}
We use Auroprobe (Janssen), marketed here by Amersham. Consistent quality.
Always works. Expensive. Some other brands work too--usually have to
use them at higher concentrations; thus, may come out just as expensive.

1 800 387 7160

I have no commercial interest in this product--just a satisfied customer.

Other sources: EMS in Pensylvania, EY in California. Can purchase less
than 1 ml at a time. Note optical density for comparison with other sources.

Use a smaller a gold if you can. 10 nm is good, if you can get away with
that size. 5 nm is a pain--hard to see; have to go up to high mag; is
gray like ferritin, not dense black.

Sara



Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: stoughma-at-ornl.gov (Matthew A. Stough)
Date: Wed, 4 Dec 1996 08:11:21 -0400
Subject: Help me Subscribe

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Can someone please send me the instructions
for SUBSCRIBING, UNSUBSCRIBING, etc. to this
Microscopy List? I have tried subscribing by
going to the MSA web site but had no luck.

Thanks!



Matthew A. Stough
HTML Graduate Fellow
Oak Ridge National Laboratory
One Bethel Valley Road
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Oak Ridge, TN 37831-6062

VOICE: 423-576-5086
FAX: 423-574-4913
email, office: stoughma-at-ornl.gov
email, school: mas157-at-psu.edu
email, home: matthewas-at-aol.com







From: stoughma-at-ornl.gov (Matthew A. Stough)
Date: Wed, 4 Dec 1996 09:55:10 -0400
Subject: Thanks...got the information I needed

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Thanks...I now have the information
required to SUBSCRIBE, UNSUBSCRIBE,
etc.

Matthew A. Stough






From: Tom Phillips :      tphillips-at-biosci.mbp.missouri.edu
Date: Wed, 4 Dec 1996 12:21:02 -0500
Subject: colloidal gold probe shelf life?

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Two recent postings have implied colloidal gold probes have a relatively
short half life. Could people comment on how long their probes are stable
and how they store them. TIA




Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(573)-882-4712 (voice)
(573)-882-0123 (fax)






From: kennedy-at-nsi.edu (Grace Kennedy)
Date: Wed, 4 Dec 1996 13:33:49 -0800
Subject: gold probe shelf life

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Three months tops for me, stored in the original container at 4C,
regardless of supplier. Definitely weaker after 6 weeks. (I've tried
Amersham, E-Y Labs, Chemicon, Biocell--Chemicon gets my vote for the best
buy.)






From: Kathy Walters :      kwalters-at-emiris.iaf.uiowa.edu
Date: Wed, 4 Dec 1996 15:37:31 -0600 (CST)
Subject: lipofuscin

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HI

I have some aged human brain tissue exibiting autofluorescence. I
susspect the culprit is lipofuscin. I seem to remember a thread where
Chip Montrose asked about lipofusion, but I don't recall if he received
any "cures". Does any one know how to block the autofluorescence of
lipofusion?

TIA,
Kathy Walters


Kathy Walters / /
Research Assistant III / /\
Center for Microscopy Research / /\ \
University of Iowa /_/ \ \
85 EMRB ____ ((O))
Iowa City, Iowa 52242 | | / /
|| / /
email: kwalters-at-emiris.iaf.uiowa.edu -----------
fax: (319)335-8049 -------------
www: http://www.uiowa.edu/~cemrf






From: bjg-at-uniwa.uwa.edu.au (Brendon J. Griffin)
Date: Thu, 5 Dec 1996 07:15:15 +0800
Subject: old Evap. coater needed

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Goodai

I am looking for an old (but operable) evaporative coater for dedicated Au
coating. The samples are for ion microprobe analysis and must be
evaporatively coated. Any offers should give an idea of price and
condition.

Thank you


Brendon J. Griffin
Centre for Microscopy and Microanalysis
The University of Western Australia
Nedlands, WA, AUSTRALIA 6907
ph 61-9-380-2739 fax 61-9-380-1087






From: Kathy Walters :      kwalters-at-emiris.iaf.uiowa.edu
Date: Wed, 4 Dec 1996 15:58:05 -0600 (CST)
Subject: Re: Cryostat Sectioning Bone

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Message-Id: {m0vVRl1-00043JC-at-fast.net}

Dear Mike,

The main problem I had when cutting mouse noses (snouts?) was getting the
sections to adhere to the slides during processing. My best results came
after reading a paper in The Journal of Histochemistry and Cytochemistry.
The article is "An Improved Method for Preparing Cryostat Sections of
Undecalcified Bone for Multiple Uses." Vol.38, No. 3. pp. 443-448.
Good Luck,
Kathy

Kathy Walters / /
Research Assistant III / /\
Center for Microscopy Research / /\ \
University of Iowa /_/ \ \
85 EMRB ____ ((O))
Iowa City, Iowa 52242 | | / /
|| / /
email: kwalters-at-emiris.iaf.uiowa.edu -----------
fax: (319)335-8049 -------------
www: http://www.uiowa.edu/~cemrf


On Tue, 3 Dec 1996, Michael J. Lyon wrote:

} I need some help in obtaining cryostat sections of undecalcified bone. I
} am using diffusible tracers and cannot decalcify the bone. Any
} suggestions would be welcomed on sectioning angle, temperature, knives,
} etc. The piece that I need to section is about 5-7 mm square and I need
} to have sections of at least 20 um in thickness.
}
} Thanks in advance
}
} Mike
}





From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 04 Dec 1996 16:35:27 -0500
Subject: Re: colloidal gold probe shelf life?

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Shelf life depends on the protein conjugated to the gold and the batch. We
use IgG conjugates for 6 months or more storing them at 4 C. I have used
protein A conjugates that were a year and a half old. Other times they have
gone bad much more quickly. It is always best to have a positive control
for activity of the gold if it has been several weeks since the last use.
If the gold is in a glycerol solution it can be frozen to extend its useful
life.

At 12:21 PM 12/4/96 -0500, you wrote:
} Two recent postings have implied colloidal gold probes have a relatively
} short half life. Could people comment on how long their probes are stable
} and how they store them. TIA
}
}
}
}
} Thomas E. Phillips, Ph.D.
} Associate Professor of Biological Sciences
} Director, Molecular Cytology Core Facility
} 3 Tucker Hall
} University of Missouri
} Columbia, MO 65211
} (573)-882-4712 (voice)
} (573)-882-0123 (fax)
}
}
}
}
*******************************************************
G.W. Erdos, Ph.D. Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*****
"Many shall run to and fro, and knowledge shall be increased"
Daniel 12:4





From: becks-at-sunynassau.edu (Steve Beck)
Date: Wed, 4 Dec 1996 20:19:44 -0500
Subject: SEM Imaging of CD4 Sites

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Dear Colleagues:

I have a couple of former EM students who would like to image the
distribution of CD4 proteins on the surface of CEM-E5 cells (immortal human
helper T-cell line). The cells are grown in a media known as RPMI (I was
informed that it's similar to the Modified Eagle Medium) and supplemented
with a bovine growth serum.

They have access to a primary antibody, OkT4, which is specific for the CD4
protein and a secondary gold labeled antibody - mouse IgG + IgM with a 12nm
gold bead.

Since I have not had much experience with immuno-EM, I would appreciate any
direction, references and advice you might have.

The specific questions I have follow:

1. Does this sound like a feasible project - can SEM be used to image such
membrane proteins as CD4 using gold labeled abs? (I have a tungsten based
Hitachi S-2400 SEM with a 4nm resolving power at 25kv - secondary electron
detector only).

2. Would a BSE detector be more useful in imaging the gold labeled abs?

3. Can you recommend a protocol (or specific reference) for fixing and
processing the cells - Is there something we could use to affix the cells
for processing - would polylysine coated coverslips be useful?

4. For drying, would CPD work well or would you recommend the
organo-silicon route (TMS, HMDS) directly from ethanol dehydration series?

5. I would assume that gold sputter coating would be contrary to our goals
- Is there a method for producing a high quality image at lower voltages OR
a method for enhancing conductivity without the need for conductive coating
[I am aware of the OTO(TO) technique for more "conventional" samples].

6. In a related project, these students would like to study the
relationship between CD4 and fusin receptors on these cells. Could these
sites be labeled differentially so as to be discerned when imaged?
In addition, are antibodies to fusin currently available.

7. Are there any additional points I haven't considered - once again, I'm
rather new to this technique!

Thanks in advance!






Stephen J. Beck
Bio-Imaging Center/Electron Microscopy
Department of Biology
Nassau Community College
Garden City, NY 11530
Voice Mail: (516) 572-7829
Email: {becks-at-sunynassau.edu
URL: {http://www.sunynassau.edu/webpages/biology/becks.htm}






From: Keith Moulding :      mcmouldk-at-uxmail.ust.hk
Date: Thu, 05 Dec 1996 11:50:09 +0800
Subject: Thin Film EDS analysis on a bulk substrate

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Hi,

Are there any programs (PC base preferred) which will do EDS analysis of a
thin film on a bulk substrate? Typical we look at ~1000 A thick films
(sometimes multilayer films) on silicon substrates. I have heard of a
program call STRATA, will this program do what I require?

I know there is the GMRFILM program in the archives, however the zip file
appears to be corrupted.

Many Thanks,

Keith.


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr. K. Moulding.

Materials Characterisation and Preparation Centre,
Hong Kong University of Science and Technology,
Clear Water Bay,
Kowloon,
Hong Kong.

FAX: (852) 2358 2451
TEL: (852) 2358 8724
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From: () (by way of Nestor J. Zaluzec)
Date: Wed, 4 Dec 1996 22:05:47 -0500
Subject: IAS Holiday Meeting/Pittsburgh

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MeetingName: IAS Holiday Meeting/Pittsburgh
MeetingDates: Dec. 12, 1996
MeetingTopic: Two presentations will be given:
John Friel, Princeton Gamma Tech,on " Low-Voltage, High-Resolution X-ray
Mapping", and
Louis Hector, Alcoa, on " Atomic Force Mircoscopy as a Tool in Materials
Science".

The IAS is a professional society providing a forum for the exchange
of information related to materials characteriztion in fields such
as metallurgy, chemistry, microscopy, life science, and biotechnology.

MeetingSponsor: Instrumentation and Analysis Society (IAS)
City: Alcoa Technical Center
State: PA
Zip: 15069
Country: Westmoreland
Interests: Physical Sciences, Scanning Probe, SEM
ContactName: Liz Goodwin or Hasso Weiland
ContactCity: Alcoa Center
ContactState: PA
ContactZip: 15068
Phone: 412 744-100 or 412 337-3133
email: goodwin-at-sgi.net or weiland_h-at-atc.alcoa.com
---------------------------------------------------------------------------






From: Doug Bray :      wangl000-at-HG.ULETH.CA
Date: Wed, 04 Dec 1996 22:56:13 -0700
Subject: neurogenesis of subventricular zone

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Message-ID: {32A663FB.48C0-at-hg.uleth.ca}

G'Day.
Does anyone have any information regarding neurogenesis in the
subventricular zone? I have a project due and I can't find any up to
date information. I have micrographs from the SEM, but I am unsure what
exactly I am looking at.

Thanks in advance for anyone who can help me.


Christopher L.C. Wang
Dept. of Bio. Sci.
University of Lethbridge
Tel: (403) 329-2210




From: jjha-at-202.30.60.37 (Jong Jae Ha)
Date: Thu, 5 Dec 1996 15:48:40 +0900
Subject: Subscribe

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sunscribe





From:
Date: Wed, 4 Dec 1996 22:05:47 -0500
Subject: IAS Holiday Meeting/Pittsburgh

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------------------------------------------------------------------------------





From: Daniele Spehner :      daniele.spehner-at-etss.u-strasbg.fr
Date: Thu, 05 Dec 1996 13:27:22 +0100
Subject: Re: TEM-protein A gold

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Message-ID: {32A6BFAA.3327-at-etss.u-strasbg.fr}

We use Aurion. It is the name of a Belgian society managed by Jan
Leunissen. Jan Leunissen, Ph.D.
AURION ImmunoGold Reagents & Accessories
Costerweg 5, 6702 AA Wageningen
The Netherlands

phone (31)-317-497676
fax (31)-317-415955
I think that the Aurion products are commercialised by EMS in the USA.
I have no commercial interest in the gold conjugates they are selling, I
am just satisfied, not only by the products but also by the advices and
discussions.




From: Leah Dobbs :      leadob-at-execpc.com
Date: Thu, 5 Dec 1996 06:48:42 -0000
Subject: thank you for diffraction answer's

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Message-Id: {199612051248.GAA15027-at-mailgate.execpc.com}

Thank you to every one who contributed to my question about exposure time
for Diffraction Patterns. The information was very useful.

Leah L Dobbs
Madison Area Technical College




From: GREG VAUGHN MARTIN :      gmartin-at-welchlink.welch.jhu.edu
Date: Thu, 5 Dec 1996 07:46:05 -0500 (EST)
Subject: Re: colloidal gold probe shelf life?

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Hey folks --

We use gold probes from Jackson exclusivly. Stored at 4C, we get
excellent results for at least one year -- I have one batch of anti-HRP
gold that is three years old and still works fine (by eye). I have not
done a quantitative analysis on decrease in labeling over time. We also
see a batch-to-batch variation in shelf life.

Greg Martin
Dept. of Cell Biology and Anatomy
Johns Hopkins School of Medicine







From: DDKJoe-at-aol.com
Date: Thu, 5 Dec 1996 10:28:51 -0500
Subject: Re: Cryostat Sectioning Bone

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Mike,

There is a protocol with references on our website at
http://www.ddk.com/cryobone.html

You email me from there if you have additional questions.

Good luck,
Joe Tabeling
Delaware Diamond Knives




From: kennedy-at-nsi.edu (Grace Kennedy)
Date: Thu, 5 Dec 1996 08:08:53 -0800
Subject: neurogenesis

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I'm not exactly sure of what you're looking for, but the following paper
may prove interesting even though the study was done in fish:

Zupanc, Gunther K.H., and Horschke, Ingrid (1995) Proliferation zones in
the brain of adult Gymnotiform fish: A quantitative mapping study.
J.Comp.Neurol. 353:213-233.
.






From: Linda Fox :      lfox1-at-wpo.it.luc.edu
Date: Thu, 05 Dec 1996 10:12:37 -0600
Subject: INK - Carnoy's

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Message-Id: {s2a6a332.077-at-wpo.it.luc.edu}
X-Mailer: Novell GroupWise 4.1

Help Please,
Does anyone know of a way to recover writing from Histo tissue
holders that have been processed in Carnoy's? Details as follows:
Holders were marked with, Securline MarkerII/Superfrost (permanent,
solvent-resistant)
SP Cat No P1220, then placed in Carnoy's fixative, (Absolute
alcohol, Chloroform and glacial acetic acid.) All of the writing
seems to have dissolved.
Are there any forensic scientists or others with experience that may
help us to recover some very valuable tissue? All suggestions are
most welcome. Thanks Linda Fox Loyola University Medical Center
lfox1-at-wpo.it.luc.edu, phone 1-708-216-3395, fax 1-708-216-3913





From: ebs-at-ebsciences.com
Date: Thu, 05 Dec 1996 08:48:08
Subject: Re: colloidal gold probe shelf life?

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Dear Tom and colleagues,

The BioSite colloidal gold probes which we sell will keep for about a year
in the refrigerator, and for an indefinite period of time when frozen. Most
of our customers will aliquot the reagent, and store the small volumes in
the freezer until needed. The probes are available in 1ml and .25ml volumes.

Best regards,
Steven E. Slap, Vice-President
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: carol-at-csbnmr.health.ufl.edu (Carol Tsekouras) (by way of Greg Erdos :      gwe-at-biotech.ufl.edu )
Date: Thu, 05 Dec 1996 08:49:24 -0500
Subject: Position Open

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OPTICAL MICROSCOPIST POSITION:

Immediate opening for Assistant-in or Assistant Scientist to operate and
maintain confocal and deconvolution microscopes in Center for Structural
Biology. This position will involve user support and collaborative research
with investigators from various departments. Final rank and starting
salary are commensurate with education and experience. Candidates should
have M.S. or Ph.D. with experience, and be knowledgable in use of advanced
optical
microscopies, and in their application to biological and medical research.
Anticipated starting date February 1, 1997. Send CV by
January 20, 1997 to Dr. Thomas H. Mareci, Center for Structural Biology, PO Box
100245, University of Florida, Gainesville, FL 32610. The University is an
equal employment opportunity/affirmative action employer.









From: Doug Bray :      wangl000-at-HG.ULETH.CA
Date: Wed, 04 Dec 1996 22:56:13 -0700
Subject: neurogenesis of subventricular zone

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Does anyone have any information regarding neurogenesis in the
subventricular zone? I have a project due and I can't find any up to
date information. I have micrographs from the SEM, but I am unsure what
exactly I am looking at.

Thanks in advance for anyone who can help me.


Christopher L.C. Wang
Dept. of Bio. Sci.
University of Lethbridge
Tel: (403) 329-2210




From: nano-at-ns1.lihti.org (Nanoprobes Inc.)
Date: Thu, 5 Dec 1996 11:07:19 -0500
Subject: Re: Gold Probe Shelf Life

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Kramercy and Sealock (J. Histochem and Cytochem, 1991, Vol. 39 p. 37-39)
investigated the presence of unconjugated antibody in colloidal gold
preparations and found some even in some "recently delivered" (they didn't
say how long) samples. They recommended storing colloidal gold conjugates
frozen, and testing them for free antibody before use by pelleting out the
gold, labeling a sample with a dilution of the gold-free supernatent
solution, then detecting the bound unconjugated IgG (in their case from
goat) in the sample using a fluorochrome-labeled probe.

Gel filtration or size exclusion chromatography may be used to separate the
unattached antibody from larger gold conjugates instead of pelleting and
resuspending, and this also has the advantage of also removing aggregates
(our colloidal gold probes are purified this way). I've also heard from a
couple of sources that Jackson's 6 and 12 nm gold conjugates are very
stable compared with other manufacturers.

Our 1.4 nm Nanogold=81 gold cluster conjugates are covalently cross-linked t=
o
the conjugate antibody rather than electrostatically adsorbed: this has
been shown to give much improved stability. In our tests, these work
identically after 12 months at 4=B0C, and have been used up to 2 years after
preparation. These give superior cellular penetration and more quantitative
labeling than equivalent colloidal gold conjugates (Vandr=E9 and Burry, J.
Histochem. Cytochem, 1992 Vol. 40 p. 1837; Takizawa and Robinson, J.
Histochem. Cytochem, 1994 Vol. 42 p. 1615). We cannot (yet) offer covalent
conjugates with larger gold labels, but Nanogold=81 may be effectively silve=
r
enhanced.

Hope this is useful,

Rick Powell



******************************************************************
* NANOPROBES, Incorporated | Tel: (516) 444-8815 *
* 25 East Loop Road, Suite 124 | Fax: (516) 444-8816 *
* Stony Brook, NY 11790-3350, USA | nano-at-mail.lihti.org *
* *
* NOW EASY TO FIND ON THE WEB: http://www.nanoprobes.com *
******************************************************************






From: Brad Goodwin :      goodwib-at-wdni.com
Date: Thu, 05 Dec 1996 14:34:45 -0800
Subject: LR White "ripples" in section

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I'm having problems with LR White sections. Fixation of plant embryo
material is fixed using 3% glutaraldehyde and buffer at 0 degrees
overnight. Dehydration is from buffer through ethanol series to
n-butanol. LR White and tissue are hardened by heating at 55 degrees C
for 24 hours in a Beem capsule. Sectioning is on a Reichert:Jung 2040
using a carbide tungsten blade at 3 microns. Sections are placed on a
drop of water before air drying. Staining consists of 5 min in 1% Acid
Fushin, rinse, 5 min in 0.05% toluidine blue O in benzoate buffer, final
rinsing, air drying and the addition of CytoSeal 60 mountant and a cover
slip.

Problems start at the staining. Many sections float away, although I
thought plastic sections "stuck". The end product has "ripples", but
the section itself is not uneven. The staining looks great, but I can't
photograph wavy sections. The sections do not seem to have "ripples"
before staining, however the waviness may not be severe enough to notice
before staining.

The protocol I'm trying to use was designed for GMA, not LR White, but
I'd hoped to just substitute the less toxic choice.

Any help is greatly appreciated. Send responses directly to
goodwib-at-wdni.com.




From: r.g.white-at-sci.monash.edu.au (Rosemary White)
Date: Thu, 05 Dec 1996 17:25:20 -0500
Subject: Confocal research assistant/research fellow

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NOTE: This is a re-posting - the closing date has been changed by higher
authorities to 3 January 1997...

RESEARCH ASSISTANT/RESEARCH FELLOW LEVEL A

We require an experienced person to set up and manage a new
multi-institution confocal facility at Monash University in Melbourne, to
be shared with groups from the Victoria University of Technology and CSIRO,
located in the Department of Ecology and Evolutionary Biology. The
facility will include both upright and inverted microscopes plus a
stand-alone image analysis workstation.

Experience in use and maintenance of confocal microscope hardware and
software is essential. Experience in a broad range of confocal
applications in biology is highly desirable, so that the appointee could
assist users in selecting the best technique for their particular project,
and bring new applications to their notice on a regular basis. Familiarity
with 3D reconstruction and image analysis applications is also desirable.

The position is available for one year in the first instance, with
anticipated starting date of 1 March, 1997. Salary within the range
$30,130 to $40,889 ($38,092 minimum with PhD) depending on experience.
During 1997, users will require training in use of the microscope, and
management protocols will be established. The appointee will be part of a
small management committee representing the major user groups.

For further details, contact Dr. David Smyth, Department of Genetics and
Developmental Biology, email David.Smyth-at-sci.monash.edu.au, ph.
61-3-9905-3861, fax 61-3-9905-5537 or Dr. Rosemary White, email
r.g.white-at-sci.monash.edu.au.

Please send applications, including CV and names of three referees, to Ms.
Annabel Carle, Department of Ecology and Evolutionary Biology, Monash
University, Clayton, Victoria 3168, Australia.

Closing date for applications is 3 January, 1997.

______________________________________________________________________
**********************************************************************

Rosemary White
Department of Ecology and Evolutionary Biology
Monash University, Clayton, Victoria 3168, Australia
phone 61-3-9905 5670 email r.g.white-at-sci.monash.edu.au
fax 61-3-9905 5613 or rgwhite-at-vaxc.cc.monash.edu.au






From: Jim Darley :      p&s-at-ultra.net.au
Date: Fri, 06 Dec 1996 22:14:44 +1100
Subject: Re: LR White "ripples" in section

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Message-Id: {1.5.4.32.19961206111444.00674630-at-mailhost.ultra.net.au}
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At 14:34 5/12/96 -0800, you wrote:
} I'm having problems with LR White sections. Fixation of plant embryo
} material is fixed using 3% glutaraldehyde and buffer at 0 degrees
} overnight. Dehydration is from buffer through ethanol series to
} n-butanol. LR White and tissue are hardened by heating at 55 degrees C
} for 24 hours in a Beem capsule. Sectioning is on a Reichert:Jung 2040
} using a carbide tungsten blade at 3 microns. Sections are placed on a
} drop of water before air drying. Staining consists of 5 min in 1% Acid
} Fushin, rinse, 5 min in 0.05% toluidine blue O in benzoate buffer, final
} rinsing, air drying and the addition of CytoSeal 60 mountant and a cover
} slip.
}
} Problems start at the staining. Many sections float away, although I
} thought plastic sections "stuck". The end product has "ripples", but
} the section itself is not uneven. The staining looks great, but I can't
} photograph wavy sections. The sections do not seem to have "ripples"
} before staining, however the waviness may not be severe enough to notice
} before staining.
}
} The protocol I'm trying to use was designed for GMA, not LR White, but
} I'd hoped to just substitute the less toxic choice.
}
} Any help is greatly appreciated. Send responses directly to
} goodwib-at-wdni.com.
*******************************************
Dear goodwib et al -
Try this: Plastic sections stick much better if they are heated. If heated
in a drop of water they are likely to stretch better too. This may be the
answer to those wrinkles too, but some very thick plant cell walls can be
difficult in that respect.
Within reason more heat causes the sections to stick better. Try 80 degrees
for at least a couple of minutes after the water has evaporated.
Use a single drop of the stains and wash off gently using squeeze bottle or
a Pasteur pipette. Wash into a small dish, then, if needed you could
retrieve the section and place it back onto a slide using a wire loop. For
a big section use a wedge shaped piece of coverslip.
Drawing a circle on the underside of the slide makes locating small sections
easier.
Hope this helps others with these common problems too.
Cheers
Jim Darley
Probing & Structure (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site: http://www.ultra.net.au/~pns/





From: Daniele Spehner :      daniele.spehner-at-etss.u-strasbg.fr
Date: Fri, 06 Dec 1996 13:08:33 +0100
Subject: re : colloidal gold

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Message-ID: {32A80CC1.1C04-at-etss.u-strasbg.fr}

"Hi there!
"Can anyone recommend a commercial source of good quality protein A
"gold (20 nm).
"Thanks
"Dorota

I buy all my conjugate antibodies by Aurion ( sold by EMS in the USA)
and I am really satisfied with. Aurion is a small compagny managed by
Dr. Jan Leunissen. Many of you may know him through the books that he
has written on Immunogold, and the courses that he teaches on the
subject.Aurion Immunogold reagents have a guaranteed shelf life of 18
months from the date of the quality control analysis.

Jan Leunissen, Ph.D.
AURION ImmunoGold Reagents & Accessories
Costerweg 5, 6702 AA Wageningen
The Netherlands

phone (31)-317-497676
fax (31)-317-415955




From: nyao-at-princeton.edu (Nan Yao)
Date: Fri, 6 Dec 1996 10:07:50 -0500
Subject: Electron Microscopes Available

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We have two electron microscopes for sale: 1) a Philips 400T TEM/STEM, with
a total operation time less than 200 hours; 2) a Dedicated VG HB-501A
STEM, which is about 9 years old and has all possible attachments on it.



Nan Yao
Princeton Materials Institute
Princeton University
Bowen Hall, 70 Prospect Ave.
Princeton, New Jersey 08540-5211

Tel: (609) 258-6394
Fax: (609) 258-6878
Email: nyao-at-princeton.edu






From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Fri, 6 Dec 1996 07:57:10 -0800 (PST)
Subject: Re: LR White "ripples" in section

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Hello,
If drying down the section on a warming tray doesn't do it, we sometimes
use charged slides to help them stick quicker. But the ripples may be
from incomplete polymerization. If LR white's polymerization is
interrupted by fluctuation in temperature or introduction of oxygen it may
not restart. We always polymerize in a nitrogen atmosphere using an old
paraffin vacuum oven hooked up to dry nitrogen.

Bob
Morphology Core
Univ. of Washington

On Thu, 5 Dec 1996, Brad Goodwin wrote:

} I'm having problems with LR White sections. Fixation of plant embryo
} material is fixed using 3% glutaraldehyde and buffer at 0 degrees
} overnight. Dehydration is from buffer through ethanol series to
} n-butanol. LR White and tissue are hardened by heating at 55 degrees C
} for 24 hours in a Beem capsule. Sectioning is on a Reichert:Jung 2040
} using a carbide tungsten blade at 3 microns. Sections are placed on a
} drop of water before air drying. Staining consists of 5 min in 1% Acid
} Fushin, rinse, 5 min in 0.05% toluidine blue O in benzoate buffer, final
} rinsing, air drying and the addition of CytoSeal 60 mountant and a cover
} slip.
}
} Problems start at the staining. Many sections float away, although I
} thought plastic sections "stuck". The end product has "ripples", but
} the section itself is not uneven. The staining looks great, but I can't
} photograph wavy sections. The sections do not seem to have "ripples"
} before staining, however the waviness may not be severe enough to notice
} before staining.
}
} The protocol I'm trying to use was designed for GMA, not LR White, but
} I'd hoped to just substitute the less toxic choice.
}
} Any help is greatly appreciated. Send responses directly to
} goodwib-at-wdni.com.
}





From: chandler-at-lamar.ColoState.EDU (John Chandler)
Date: Fri, 6 Dec 1996 11:50:46 -0700
Subject: Used TEM Available

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I have been asked to post this announcement. Please do not reply to the list.

Model: JEOL EM-100SX, with water chiller
Installed: 11/86
On contract with JEOL until '89
Inspected by JEOL 4/96, fully functional at 100kV, but could use some repairs
Location: Pacific northwest, USA
Contact: Peg Baskerville, 1-800-557-0401

John
chandler-at-lamar.ColoState.EDU






From: Paul Webster :      paul.webster-at-Yale.edu
Date: 6 Dec 1996 13:53:41 -0500
Subject: Re: colloidal gold

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Message-ID: {n1362241663.78804-at-QuickMail.Yale.edu}

There has been much talk about "shelf life" of colloidal gold probes but little
explanation of how this is evaluated.

We use protein A, and other protein-gold congujates for many different
applications. For us, shelf life is determined by the final labeling efficiency
i.e. is the signal the same as when we first made/purchased the probe?

As far as I know there are no published stereological studies on the labeling
efficiency of gold probes over time. However, from experience I know that if we
try to label biotinylated cells with streptavidin-gold that is more than 5 days
old, we get no signal.

(Commercial produces of this probe need not worry because most applications
involve detection of biotinylated antibodies followed by silver intensification,
both producing colossal amplifications of the signal.)

In contrast, although I have been told on numerous occasions that protein A-gold
has a shelf life of between 3 months to a year, I have used probes that are more
than 6 years old and seen qualitative results similar to those produced using
fresh probes. Unfortunately, other protein A-gold preparations have not aged so
well.

For us there seem to have been little difference between storing the probes
frozen in glycerol or in a 'fridge in the presence of azide. However (more
dogma) I have been advised that freezing in glycerol will produce small
aggregates of 2-3 particles. Again this is not something we have noticed.

From what is published it seems fair to conclude that the stability of a
particular probe depends on the protein used to stabilize the colloid and on the
amount of protein used. If these variables can be compared and correlated with
labeling efficiency then it is worth sharing this data.

Posting opinions only serves to add further support to dogma.

Best regards,

Paul Webster, Ph.D
Center for Cell Imaging
Yale School of Medicine
http://info.med.yale.edu/cellimg





From: Damian_Neuberger_at_RLT013-at-ccmailgw.mcgawpark.baxter.com
Date: Fri, 6 Dec 1996 10:43:01 -0600
Subject: Calcium particulate ID

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Good Morning (Central Std Time USA) All

I have been asked if there is a technique (optical microscopy, SEM,
EDX, confocal (MRC1000 K/Ar laser) to determine whether calcium
carbonate particulate is adhering to the surface of mesothelial cells
(on the surface of a tissue sample). I suggested fixed tissue but CPD
would probably result in loss of at least some of the adherent
particulate. I could use cryoSEM of fixed tissue but I can't do EDX
of frozen samples on our microscope. Is there a method I could use on
the confocal? Questions that the researcher could not answer are: 1)
is there particulate known to be present (don't know); 2) what size
particulate (could be in the 10s of nanometers to hundreds of microns;
well that covers just about everything!); 3) can there be
intracellular deposits of calcium (yes, that's possible). I know that
this is not very much to go on but it's all I can bring to the
listserve. Hope someone can help and thanks in advance (TIA)

Damian Neuberger, Ph.D.
Research Scientist
Baxter International
WG3-2S, P.O.Box 490
Round Lake, IL 60073
neuberd-at-baxter.com
847.270.5888
FAX 847.270.5897




From: BobCat54-at-aol.com
Date: Fri, 6 Dec 1996 18:08:38 -0500
Subject: Re: Welcome To The Microscopy ListServer

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You have my E-mail address right... I received the mailing.

Robert Isabelle





From: Luc Nocente :      ln-at-noesisvision.com
Date: Fri, 06 Dec 1996 16:37:47 -0500
Subject: Re: EM software-need to locate

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Message-Id: {3.0.32.19961206163742.0077fe94-at-noesisvision.com}
X-Sender: ln-at-noesisvision.com
X-Mailer: Windows Eudora Pro Version 3.0 (32)

Try VisilogPro for the price of 2,995.00$US. In addition we have a new
Materials Analysis module for an extra 1,000.00$.

This module allows to rebuild grains using advanced morphology plus over a
dozen other functions related to grain sizing and imaging in microscopy.

At 10:07 AM 12/6/96 CST, MCOV%mimi-at-magic.itg.ti.com wrote:
} From: Mike Coviello MCOV
}
} Subj: EM software-need to locate
}
}
} Can anyone recommend any inexpensive software (or shareware) for: 1) Indexing
} of diffraction patterns 2) Grain-size analysis in PC or Mac format(please
} specify which in your response).
}
} Michael Coviello
} Central Research Laboratories
} Texas Instruments
} Dallas, TX
}
}


----------------------------------------------------------------------------
---------------------
Luc Nocente Tel: 514 345 1400
Noesis Vision Inc. Fax: 514 345 1575
e-mail: ln-at-noesisvision.com
6800 Cote de Liesse, Suite 200
St-Laurent, PQ
H4T 2A7,Canada

Visit our new web site at http://www.noesisvision.com
----------------------------------------------------------------------------
---------------------




From: LARNOULD J. :      larnould-at-mnet.fr
Date: Sat, 07 Dec 1996 18:50:38 +0100
Subject: High resolution printer

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Hi Everybody,
Does somebody has heard something about an enhancement for laser printers
called LAZARPRINT and
abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I know
it looks impossible)
I'm interresting by technical information and manufacturer.
Salutations.
==========================================================
Jacky Larnould
tel 33 (0)4 67 72 28 26
fax 33 (0)4 67 79 54 90
email larnould-at-mnet.fr





From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Sun, 8 Dec 1996 12:00:09 -0500
Subject: Microscopy was Down for a Day

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--------------------------------------------------------------------
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G'day Subscribers....

Sorry, but I took the system down for a day for
some system maintenance. Everything should now be
back on-line. Hopefully no-body noticed the
shutdown. If your postings over the weekend
bounced then please resend them.

Nestor
Your Friendly Neighborhood SysOp.






From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel) (by way of Nestor J. Zaluzec)
Date: Sun, 8 Dec 1996 12:26:20 -0500
Subject: Re: Calcium particulate ID

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} I have been asked if there is a technique (optical microscopy, SEM,
} EDX, confocal (MRC1000 K/Ar laser) to determine whether calcium
} carbonate particulate is adhering to the surface of mesothelial cells
} (on the surface of a tissue sample). I suggested fixed tissue but CPD
} would probably result in loss of at least some of the adherent
} particulate. I could use cryoSEM of fixed tissue but I can't do EDX
} of frozen samples on our microscope. Is there a method I could use on
} the confocal? Questions that the researcher could not answer are: 1)
} is there particulate known to be present (don't know); 2) what size
} particulate (could be in the 10s of nanometers to hundreds of microns;
} well that covers just about everything!); 3) can there be
} intracellular deposits of calcium (yes, that's possible). I know that
} this is not very much to go on but it's all I can bring to the
} listserve. Hope someone can help and thanks in advance (TIA)
}
} Damian Neuberger, Ph.D.

Since the deposits survive fixation, you can dry the cells in
hexamethyldisilizane, which is mechanically very gentle. Might give better
morphology as well.
After dehydration in EtOH, transfer to HMDS through a
2:1 EtOH:HMDS
1:2
series,
then 3X100% HMDS using the same times as for 100% EtOH;
dry at room temperature or 60 degree (or 45 degree) C oven (the
different temperatures can give different results)
Phil

&&& Illigitimi non carborundum &&&&&&&&
Philip Oshel
Microscopy
Station A
PO Box 5037
Champaign, IL 61825-5037
(217)244-3145 days
(217)355-3145 evenings
oshel-at-ux1.cso.uiuc.edu
*** looking for a job again ******************






From: LARNOULD J. :      larnould-at-mnet.fr (by way of Nestor J. Zaluzec)
Date: Sun, 8 Dec 1996 12:26:11 -0500
Subject: High resolution printer

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Hi Everybody,
Does somebody has heard something about an enhancement for laser printers
called LAZARPRINT and
abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I know
it looks impossible)
I'm interresting by technical information and manufacturer.
Salutations.
==========================================================
Jacky Larnould
tel 33 (0)4 67 72 28 26
fax 33 (0)4 67 79 54 90
email larnould-at-mnet.fr






From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Sun, 8 Dec 1996 15:04:36 -0500
Subject: Administrivia: Check your Email Filters....

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G'day Again Colleagues...

It just occured to me, that in the new configuration
of the Listserver a few of you may have minor problems.
This warning only applies only to those users who employ
filters on their Email messages to sort and trash unwanted
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You may notice that I have recently modified the
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to always *remind* people the correct address for adding
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I bring this up because some of you will be likely
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S-bscribe ( the letter "u" omitted here)
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(I have left out the "u's" above so that everyone will see
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Since both these key words now appear in the body of *every*
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I realize that this may inconvenience a few of our subscribers but
I'm trying to find a simple way to benefit the largest body of
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Just for the record the majority (} 50%) of S-bscribe and Uns-bscribe
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Cheers....

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Your Friendly Neighborhood SysOp.







From: Paul Vanderlinden :      orion-at-infoboard.be
Date: Mon, 9 Dec 1996 08:50:44 +0100 (MET)
Subject: Re: High resolution printer

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At 18:50 07/12/1996 +0100, you wrote:
}
} Does somebody has heard something about an enhancement for laser printers
} called LAZARPRINT and=20
} abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I=
know
} it looks impossible)
}
}
Dear Microscopists,

Impossible or not, this product really exists !!!

In addition to the high quality grayscale images, the speed of the process
is also remarkable: due to a direct access to the video port of the printer
via a special interface, a picture of letter size is transferred and printed
out within the time the laser printer needs for paper skip alone (+/- 10
seconds !).

The near photographic quality obtained at a very low cost (use of plain
paper) makes the LazarPrint system the ideal solution if you want to print
pictures obtained with really high resolution grabbing systems (eg our ORION
4.2 for Windows that gives you a resolution up to 4000 x 4000 pixels and 256
gray levels).

For more information, please contact:
I.C.I. sarl (France)
Voice: + 33 384 58 02 43
Fax: + 33 384 54 03 98

E.L.I. sprl (Belgium)
Voice: +32 2 726 31 02
Fax: +32 2 726 08 65
Email: orion -at-infoboard.be


Best regards,



Paul Vanderlinden.
Sales Manager.

=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
See our web site: http://www.microscopy-uk.org.uk =20

To contact us:

E.L.I. sprl

Technical support:=20
Jean-Louis Leclef: Phone: +32 67 21 25 07 =20
Fax : +32 67 22 09 53=20
Email: jleclef-at-hypercon.com
Sales support:
Paul Vanderlinden: Phone: +32 2 726 31 02 (NEW N=B0 !!!)
Fax : +32 2 726 08 65
Email: orion-at-infoboard.be=20
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D
=
=20
=20





From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Mon, 9 Dec 1996 08:44:10 +0000 (GMT)
Subject: Re: High resolution printer

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Chere Jacky:

The little gizmoes which make a low DPI printet think it's a high DPI
printer ARE A RIP OFF. They are fiddling the system and really don't
give you any real additional information. Contact John Mackenzie at
{supervisor-at-emc.ncsu.edu} who is the expert on these matters.

Patrick Echlin
Cambridge UniversityOn Sat, 7 Dec
1996, LARNOULD J. wrote:

} Hi Everybody,
} Does somebody has heard something about an enhancement for laser printers
} called LAZARPRINT and
} abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I know
} it looks impossible)
} I'm interresting by technical information and manufacturer.
} Salutations.
} ==========================================================
} Jacky Larnould
} tel 33 (0)4 67 72 28 26
} fax 33 (0)4 67 79 54 90
} email larnould-at-mnet.fr
}
}





From: Mathy H :      mathy-at-rdmetal.ulg.ac.be
Date: Mon, 9 Dec 1996 10:02:59 +0100
Subject: Re: High resolution printer

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At 18:26 08/12/96 +0100, you wrote:
}
} Hi Everybody,
} Does somebody has heard something about an enhancement for laser printers
} called LAZARPRINT and
} abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I know
} it looks impossible)
} I'm interresting by technical information and manufacturer.
} Salutations.
} ==========================================================
} Jacky Larnould
}
}
Society : LEUTRON VISION GmbH
D-82216 Gernlinden (Germany)
FAX : (0 81 42) 4 02 19





From: John Turek :      jjt-at-vet.purdue.edu
Date: Mon, 09 Dec 1996 08:37:37 -0500
Subject: Re: High resolution printer

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At 06:50 PM 12/7/96 +0100, you wrote:
} Hi Everybody,
} Does somebody has heard something about an enhancement for laser printers
} called LAZARPRINT and
} abble to increase the resolution at 6800 DPI and 1024 Gray levels!!!(I know
} it looks impossible)
} I'm interresting by technical information and manufacturer.
} Salutations.
} ==========================================================
} Jacky Larnould
} tel 33 (0)4 67 72 28 26
} fax 33 (0)4 67 79 54 90
} email larnould-at-mnet.fr
}
}
} We use the LazarPrint print board in a HP 4+ laser printer. The board
allows the printer to print at 4800 dpi. We purchased ours from Smart
Analytical Products in Maryland, USA. However, the board is made in
Germany, and I am do know who markets the board in Europe. The board is
very fast. You can even print out multiple copies of 20MB+ image files in
the same manner you would print a text file. The normal output of the
laserjet produces a dot pattern that is easily seen if you examine a print
with a magnifying glass. If you look at the grain stucture of a picture
printed using the LazarPrint board, the dots are almost too small too seen.
Each pixel can accept 1024 gray levels, but only 256 of these will be
printed. However, this allows a great deal of contrast manipulation. The
board does an excellent job with electron micrographs. I have no commercial
interest in the board.

Regards,




John J. Turek, Ph.D.
Purdue University
Dept. of Basic Medical Sciences
Core Laboratory for Image Analysis
and Multidimensional Applications (CRISTAL)
phone: 317-494-5854
fax: 317-494-0781
email: jjt-at-vet.purdue.edu





From: Klaus-Ruediger Peters :      Peters-at-BSAC.UCHC.EDU
Date: Mon, 9 Dec 1996 10:31:44 -0500
Subject: Re: High resolution printer

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{fontfamily} {param} Times {/param} {bigger} {bigger} Patrick, understanding
some of the principles of LaserJet printing may help to draw some more
objective conclusions:


LaserJets like many other printers print in lines (lpi). The lines
represent in digital image printing lines of pixels. If the pixels are
printed squarely than each square area (of a width equal to that of
the actual line width) must be printed with a desired amount of ink
variations in order to generate the gray levels (see John Russ' book
for a nice illustration on how this is done in a LaserJet). LaserJets
and inkjets use dots as the smallest printed entity. If you would print
with 600 dpi and 100 lpi (LaserJet 4) than you could place 6 dots per
line (600/100=6), As a consequence you can maximally place 6x6 dots per
pixel or make 36 gray levels plus no-dots (white). Thus a LaserJet 4M
will print with 37 gray levels. This is just at the limit of gray level
recognition. A 1200 dpi printer which uses 100 lpi, will be able to
generate 145 gray levels per pixel (1200/100 = 12; 12x12+1=145). That's
why the Lexmark is so good because the eye can't distinguish that
amount of gray levels (beyond the contrast resolution of our eyes).
However, 100 lpi (25:100= 0.25 mm) also is just at the recognition
level of the eye with regards to spatial resolution. Print heads in
LaserJets can print much better and ,if combined with HP's superfine
ink powders, they can easily be driven at 4800 dpi on plain paper.
There are many high-resolution printers on the market which do better.
However, for comparison, print speed is as important as adequate print
resolution. LazarPrint prints 300 lines per inch (lpi) and 256 gray
levels, both surely beyond the resolution limit of the unaided eye and
thus producing "photography like" printing quality. However, in
addition, LazarPrint prints 1- 10 MB of images per page in 20 sec. (on
a conventional LaserJet).


If you like to see some proof, look at a comparison of these printers
at the following web site, where dpi/lpi/eye resolution are compared
for a 1Kx1Kx8-bit test image:


http://panda.uchc.edu/htklaus/DigiLab/Printing-ResultsL.html


Patrick, trust physics and experimental proof not emotions. I don't
like gizmos either, that's why I took the time and compared the actual
print quality. Than, I made up my mind and for now over two years I
never used my photolab again, but I printed over 20,000 images (pages
with up to 8 images each) on my LaserJet, submitted MSA and other
abstracts, made all my slides and published papers; all with the help
of my old but good LaserJet.


Address of LazarPrint developer: bm484646-at-muenchen.org (Mikel Jaeth)


I am commercially not involved in any of the mentioned resources. Best
season greetings Klaus


****************

} Chere Jacky:

}

} The little gizmoes which make a low DPI printet think it's a high DPI

} printer ARE A RIP OFF. They are fiddling the system and really don't

} give you any real additional information. Contact John Mackenzie at

} { {supervisor-at-emc.ncsu.edu} who is the expert on these matters.

}

} Patrick Echlin

} Cambridge UniversityOn Sat, 7 Dec

} 1996, LARNOULD J. wrote:

}

} } Hi Everybody,

} } Does somebody has heard something about an enhancement for laser
printers

} } called LAZARPRINT and

} } abble to increase the resolution at 6800 DPI and 1024 Gray
levels!!!(I know

} } it looks impossible)

} } I'm interresting by technical information and manufacturer.

*************



{/bigger} {/bigger} {/fontfamily}

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

x Bald ist es wieder so weit (It will be happening soon)
x x
x

x * { {|:-)= Santa Claus...
x

x
x

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx







From: Laurent.NORMAND-at-ifp.fr
Date: 9 Dec 1996 16:21:38 +0000
Subject: Tests TEM

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converted ((1) (0) (10021) (7) (1) (0) (1), (1) (0) (10021) (7) (1) (0) (6));
Relayed; 9 Dec 1996 17:18:07 +0100
X400-Received: by mta area3.rueilmta in /PRMD=ifp/ADMD=atlas/C=fr/;
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Relayed; 9 Dec 1996 16:21:38 +0000

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Dear all,

I have allready sent this message last week, but I am not sur I did it right...
So I am going to bother you once more... Sorry for those who have already
received this request.

I am trying to get some information on any kind of relevant tests that can be
done to evaluate TEM performances. I want to be sur I gonna use the right method
and I gonna do it right !!

Does any body know by heart articles or books giving (practical and theoritical)
details on specific methods to test resolution (calculation of Cs..),
information limit (like ODM + Young Franges...), stability, drift, analytical
performances ??..

If you have any good advises on evaluating a TEM before buying it, I will
appreciate...

Thanks to all and to Nestor.





From: Mike Bench :      bench-at-cems.umn.edu
Date: Mon, 9 Dec 1996 12:58:22 -0600
Subject: Re: EM fields

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In resonse to the most recent discussion on magnetic fields, I thought I might
be able to add some insight from the experiences we have had minimizing them in
our facility. As a little background, our facility was built about 5 years ago
and was "designed" specifically to house our electron microscopes. From the
beginning we had magnetic field problems in some of our microscope rooms (but
not others), with field strengths as high as 3-4 mG at a couple of the
microscopes. In the service corridor that runs behind the microscope rooms
(power supplies, water chillers, elecrtrical breaker boxes, etc. are located
there) field strengths greater than 20 mG were measured near some of the breaker
boxes. These fields did not go away even if all of the breakers were tripped.
They may have decreased somewhat but they certainly were not reduced to
acceptable levels. In fact, tripping the switches at the main distribution panel
to cut off the power to our entire facility simultaneously did not cause the
fields to go away. We muddled around with several attempted fixes such as having
electricians replace sections of electrical conduit with plastic tubing and
putting rubber insulators around the conduit where it was mounted into the walls
or ceilings. We had various degrees of success with these "fixes" and some may
have made things worse.
After meeting directly with an outside consultant we were able to get a good
understanding of what was necessary to eliminate the fields. Our problems were
entirely due to there being paths to ground other than the ground wires
themselves so that the ground did not run back to the electrical box. When this
happens there is a net electrical current on the conduit and wires. It is this
net current that gives rise to the electromagnetic fields. If I remember
correctly the magnetic field associated with this current drops off as a
function of 1/r. This is compared to the fields originating from electronic
equipment that drop off as a function of 1/(r^3). I think you will also see this
1/r cubed response if you measure the field from a power cord that has no net
current on it.
In my opinion the most important tool for tracking down the sources of the
fields is an AC current probe. A number of different models are available that
plug into a digital multimeter. Ours cost about $120 from Grainger. It works by
simply clamping it around the wire or conduit you want to check. If the current
readout is not zero you have a ground path somewhere that needs to be
eliminated. A magnetic field meter is also useful for general monitoring of
field strengths and tracking down sources behind walls and other inaccessible
places. Our meter is a Holaday Industries HI-3624A that we paid under $500 for
from Holaday Industries, Eden Prairie, MN ph.612-934-4920 (no financial
interest, just a satisfied user of a product from a local company).
In the end we were able to eliminate almost all of our electromagnetic field
sources and the readings at the scopes are down to about 0.2 mG. If I were
planning a new facility I would insist that isolated ground recepticles and
switches be used everywhere. In the typical recepticle there is a place to
attach a ground wire but it is not electrically isolated from the mounting yoke
of the recepticle. So, in our case where the recepticles were mounted into a
metal wire mold that in turn was screwed into the metal studs used for framing
the rooms we ended up with all kinds of possible ground paths. In this instance
we were able to isolate the wire mold from the framing using nylon washers
around all of the mounting screws, but could have had to replace all of our
recepticles (at much greater expense). The wiring to the light fixtures was also
a major field source. In this instance, our fixtures themselves could not be
isolated so the entire conduit run back to the electrical box had to be isolated
from possible alternative ground paths. This is where the current probe was most
useful. By checking the conduit on both sides of the possible grounding points
it could be quickly determined if isolation was necessary, and if so rubber
bushings could be installed. In regard to the observance of fields when power
was "disconnected," tripping the switch simply breaks the hot wire and a current
can still flow along the neutral wire to the unintended grounding points. When
we first observed this we thought the field source may have been external to our
electrical system and would require active EM field cancellation systems ($$$).
We do still have a number of very of minor field sources, but they haven't been
worth the effort to eliminate.
For all you who have also had to deal with EM fields I hope this was of a
little help.
Mike

Mike Bench
Characterization Facility
Center for Interfacial Engineering
University of Minnesota
Voice: (612) 624-6590
Fax: (612) 626-7530
e-mail: bench-at-cems.umn.edu





From: :     
Date: Mon, 9 Dec 1996 14:23:31 -0500 (EST)
Subject: Returned mail: Host unknown (Name server: sparc5.microscopy: h

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From: wong-at-msg.ucsf.edu (Mei Lie Wong)
Date: Mon, 9 Dec 1996 11:36:23 -0700
Subject: MT2 and MT2B

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Forwarded to: smtp-at-eye-at-servers[microscopy-at-sparc5.microscopy.com]
cc:
Comments by: Yuhui Xu-at-RES-at-DFCI

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We have an MT2 (working) and an MT2B (needs new o-rings installed, there
are some which are available with the microtome) Sorvall Microtomes which
are available for sale or to the highest bidder. If you have any
questions, or would like more information, You can call, fax or email me at
the numbers listed below. 12/9/96

Mei Lie Wong
Department of Biochemistry
HHMI-UCSF
Ph. 415-476-4441 Fax 415-476-1902
email wong-at-msg.ucsf.edu






From: Ann-Fook Yang (Ann-Fook Yang) :      YANGA-at-em.agr.ca
Date: Mon, 09 Dec 1996 15:46:19 -0500
Subject: Re: High resolution printer -Reply

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I like to save your message in my computer but I have difficulties. I am
using WordPerfect 7; it does not recognize what format the text is. Can
you kindly resend it with Ascii or a wordperfect formats? Thank you
kindly.

Ann Fook




From: Ann-Fook Yang (Ann-Fook Yang) :      YANGA-at-em.agr.ca
Date: Mon, 09 Dec 1996 15:54:54 -0500
Subject: Re: High resolution printer -Reply

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I sent you a message just few minutes ago asking you to resend your
message. I misquoted the version of my WordPerfect. It is a WP 6.1 not 7.
Sorry.

Ann Fook




From: William Tivol :      tivol-at-wadsworth.org
Date: Mon, 9 Dec 1996 16:18:25 -0500 (EST)
Subject: Re: Tests TEM

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} I am trying to get some information on any kind of relevant tests that can be
} done to evaluate TEM performances. I want to be sur I gonna use the right method
} and I gonna do it right !!
}
} Does any body know by heart articles or books giving (practical and theoritical)
} details on specific methods to test resolution

The simplest method is to image a gold film, find suitably fine spa-
cings, and see what is the finest which can be resolved. Another method is
to take a through-focus series of a holey amorphous carbon film and measure
the interference fringe width at focus. The latter is a very good test of
overall performance.

(calculation of Cs..),

O. Krivanek has a procedure using amorphous C or Ge. Take images at
several values of defocus. The fringe spacings are related to Cs, and one
can use least-squares to fit the spacings at various defocus values to Cs.
There is an article on this in Ultramicroscopy (I do not have it in front
of me, but I could look up the volume & pages if you wish). Good luck.
Yours,
Bill Tivol




From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 10 Dec 1996 09:09:07 +0100
Subject: Re: High resolution printer

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X-Mailer: Mail*Link SMTP-QM 3.0.1
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From: NICK SCHRYVERS :      nick_schryvers-at-ruca.ua.ac.be
Date: 10 Dec 1996 09:09:07 +0100
Subject: Re: High resolution printer

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From: Klaus-Ruediger Peters :      Peters-at-BSAC.UCHC.EDU
Date: Mon, 9 Dec 1996 10:31:44 -0500
Subject: Re: High resolution printer

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{fontfamily} {param} Times {/param} {bigger} {bigger} Patrick, understanding
some of the principles of LaserJet printing may help to draw some more
objective conclusions:


LaserJets like many other printers print in lines (lpi). The lines
represent in digital image printing lines of pixels. If the pixels are
printed squarely than each square area (of a width equal to that of
the actual line width) must be printed with a desired amount of ink
variations in order to generate the gray levels (see John Russ' book
for a nice illustration on how this is done in a LaserJet). LaserJets
and inkjets use dots as the smallest printed entity. If you would print
with 600 dpi and 100 lpi (LaserJet 4) than you could place 6 dots per
line (600/100=6), As a consequence you can maximally place 6x6 dots per
pixel or make 36 gray levels plus no-dots (white). Thus a LaserJet 4M
will print with 37 gray levels. This is just at the limit of gray level
recognition. A 1200 dpi printer which uses 100 lpi, will be able to
generate 145 gray levels per pixel (1200/100 = 12; 12x12+1=145). That's
why the Lexmark is so good because the eye can't distinguish that
amount of gray levels (beyond the contrast resolution of our eyes).
However, 100 lpi (25:100= 0.25 mm) also is just at the recognition
level of the eye with regards to spatial resolution. Print heads in
LaserJets can print much better and ,if combined with HP's superfine
ink powders, they can easily be driven at 4800 dpi on plain paper.
There are many high-resolution printers on the market which do better.
However, for comparison, print speed is as important as adequate print
resolution. LazarPrint prints 300 lines per inch (lpi) and 256 gray
levels, both surely beyond the resolution limit of the unaided eye and
thus producing "photography like" printing quality. However, in
addition, LazarPrint prints 1- 10 MB of images per page in 20 sec. (on
a conventional LaserJet).


If you like to see some proof, look at a comparison of these printers
at the following web site, where dpi/lpi/eye resolution are compared
for a 1Kx1Kx8-bit test image:


http://panda.uchc.edu/htklaus/DigiLab/Printing-ResultsL.html


Patrick, trust physics and experimental proof not emotions. I don't
like gizmos either, that's why I took the time and compared the actual
print quality. Than, I made up my mind and for now over two years I
never used my photolab again, but I printed over 20,000 images (pages
with up to 8 images each) on my LaserJet, submitted MSA and other
abstracts, made all my slides and published papers; all with the help
of my old but good LaserJet.


Address of LazarPrint developer: bm484646-at-muenchen.org (Mikel Jaeth)


I am commercially not involved in any of the mentioned resources. Best
season greetings Klaus


****************

} Chere Jacky:

}

} The little gizmoes which make a low DPI printet think it's a high DPI

} printer ARE A RIP OFF. They are fiddling the system and really don't

} give you any real additional information. Contact John Mackenzie at

} { {supervisor-at-emc.ncsu.edu} who is the expert on these matters.

}

} Patrick Echlin

} Cambridge UniversityOn Sat, 7 Dec

} 1996, LARNOULD J. wrote:

}

} } Hi Everybody,

} } Does somebody has heard something about an enhancement for laser
printers

} } called LAZARPRINT and

} } abble to increase the resolution at 6800 DPI and 1024 Gray
levels!!!(I know

} } it looks impossible)

} } I'm interresting by technical information and manufacturer.

*************



{/bigger} {/bigger} {/fontfamily}

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

x Bald ist es wieder so weit (It will be happening soon)
x x
x

x * { {|:-)= Santa Claus...
x

x
x

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx




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From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Tue, 10 Dec 1996 08:58:16 +0000 (GMT)
Subject: Re: High resolution printer

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Dear Klaus: Thank you for your patient explanation of Laser Jet
printing. We tried these gizmo's about two years ago and at that time
could see no improvement. We now use a 600dpi pinter of a clay covered
brilliant white paper using superfine print powder and are satisfied
with the results. Perhaps things have changed in the past two years.

Sincereley

Patrick

On
Mon, 9 Dec 1996, Klaus-Ruediger Peters wrote:

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} the Microscopy Society of America
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}
} {fontfamily} {param} Times {/param} {bigger} {bigger} Patrick, understanding
} some of the principles of LaserJet printing may help to draw some more
} objective conclusions:
}
}
} LaserJets like many other printers print in lines (lpi). The lines
} represent in digital image printing lines of pixels. If the pixels are
} printed squarely than each square area (of a width equal to that of
} the actual line width) must be printed with a desired amount of ink
} variations in order to generate the gray levels (see John Russ' book
} for a nice illustration on how this is done in a LaserJet). LaserJets
} and inkjets use dots as the smallest printed entity. If you would print
} with 600 dpi and 100 lpi (LaserJet 4) than you could place 6 dots per
} line (600/100=6), As a consequence you can maximally place 6x6 dots per
} pixel or make 36 gray levels plus no-dots (white). Thus a LaserJet 4M
} will print with 37 gray levels. This is just at the limit of gray level
} recognition. A 1200 dpi printer which uses 100 lpi, will be able to
} generate 145 gray levels per pixel (1200/100 = 12; 12x12+1=145). That's
} why the Lexmark is so good because the eye can't distinguish that
} amount of gray levels (beyond the contrast resolution of our eyes).
} However, 100 lpi (25:100= 0.25 mm) also is just at the recognition
} level of the eye with regards to spatial resolution. Print heads in
} LaserJets can print much better and ,if combined with HP's superfine
} ink powders, they can easily be driven at 4800 dpi on plain paper.
} There are many high-resolution printers on the market which do better.
} However, for comparison, print speed is as important as adequate print
} resolution. LazarPrint prints 300 lines per inch (lpi) and 256 gray
} levels, both surely beyond the resolution limit of the unaided eye and
} thus producing "photography like" printing quality. However, in
} addition, LazarPrint prints 1- 10 MB of images per page in 20 sec. (on
} a conventional LaserJet).
}
}
} If you like to see some proof, look at a comparison of these printers
} at the following web site, where dpi/lpi/eye resolution are compared
} for a 1Kx1Kx8-bit test image:
}
}
} http://panda.uchc.edu/htklaus/DigiLab/Printing-ResultsL.html
}
}
} Patrick, trust physics and experimental proof not emotions. I don't
} like gizmos either, that's why I took the time and compared the actual
} print quality. Than, I made up my mind and for now over two years I
} never used my photolab again, but I printed over 20,000 images (pages
} with up to 8 images each) on my LaserJet, submitted MSA and other
} abstracts, made all my slides and published papers; all with the help
} of my old but good LaserJet.
}
}
} Address of LazarPrint developer: bm484646-at-muenchen.org (Mikel Jaeth)
}
}
} I am commercially not involved in any of the mentioned resources. Best
} season greetings Klaus
}
}
} ****************
}
} } Chere Jacky:
}
} }
}
} } The little gizmoes which make a low DPI printet think it's a high DPI
}
} } printer ARE A RIP OFF. They are fiddling the system and really don't
}
} } give you any real additional information. Contact John Mackenzie at
}
} } { {supervisor-at-emc.ncsu.edu} who is the expert on these matters.
}
} }
}
} } Patrick Echlin
}
} } Cambridge UniversityOn Sat, 7 Dec
}
} } 1996, LARNOULD J. wrote:
}
} }
}
} } } Hi Everybody,
}
} } } Does somebody has heard something about an enhancement for laser
} printers
}
} } } called LAZARPRINT and
}
} } } abble to increase the resolution at 6800 DPI and 1024 Gray
} levels!!!(I know
}
} } } it looks impossible)
}
} } } I'm interresting by technical information and manufacturer.
}
} *************
}
}
}
} {/bigger} {/bigger} {/fontfamily}
}
} xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
}
} x Bald ist es wieder so weit (It will be happening soon)
} x x
} x
}
} x * { {|:-)= Santa Claus...
} x
}
} x
} x
}
} xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
}
}
}
}





From: Michel Warnau :      mwarnau-at-ulb.ac.be
Date: Tue, 10 Dec 1996 10:22:18 +0100
Subject: Regional newspapers

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zlbacb-at-zoo.upe.ac.za, rbirenhe-at-bio.titech.ac.jp, cndmdn-at-csi.unimi.it,
capborza-at-cce.ufpr.br, tab-at-hits.net, gonzalomo-at-lcg.servicom.es,
anna-at-anatomy.su.oz.au, zocp-at-ccvax.sinica.edu.tw, bofschia-at-usthk.ust.hk,
egel-at-lotus.univ-corse.fr, fujisawa-at-sacs.sv.saitama-u.ac.jp,
fujita-at-kahaku.go.jp, d-fujita-at-nsknet.or.jp, faribm-at-visenet.iasnet.com,
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Dear colleagues, Dear friends,
We (Ali Temara and Michel Warnau) became new young fathers in the
course of November 1996. We'd like first to announce it to the world as
we are incredibly proud of our sons (Rayan and Nathan, respectively).
Then we would like to ask to all of our colleagues and friends around
the world to try to find the newspaper that was published on the day of
their birth (November 17th and November 27th). Indeed, it is likely that
you and/or a member of your family or a colleague keep newspapers without
any particular aim and throw them away once a month. We would especially
appreciate the front page of your regional newspapers.
We take the opportunity of this message to wish you a Merry Christmas
and a happy and prosperous New Year!
Kindest regards,

Ali & Michel

________________________________________________________________
Dr. Ali Temara and Dr. Michel Warnau

Laboratoire de Biologie Marine (CP 160/15)
av. F.D. Roosevelt 50
B-1050 Brussels (BELGIUM)

Phone: 32/2/650 29 70 - 32/2/650 22 34 Fax: 32/2/650 27 96
e-mail: atemara-at-ulb.ac.be
mwarnau-at-ulb.ac.be





From: Walter A. Mannheimer :      wamann-at-metalmat.ufrj.br
Date: Tue, 10 Dec 1996 11:36:26 EST3EDT
Subject: zip drive

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Hello fellow microscopists,
Does anyone have experience in running an Iomega ZIP drive under
Novell 7 DOS on a network?
My computer Pentium 100, Windows 3.1 hangs when running the guest
driver;
if I boot on MS DOS 6.2 the drive runs fine.
Have not been able to get help from Iomega on this.

Thank you, and Season's Greetings
Prof.Walter A.Mannheimer
Metallurgy and Materials Engineering
Federal University of Rio de Janeiro
POBox 68505 21945 Rio de Janeiro, Brazil
Voice +5521 280-7443 (Dept.office) +5521 590-0579 (direct)
Fax +5521 290-6626 Email: wamann-at-metalmat.ufrj.br




From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Tue, 10 Dec 1996 07:48:51 -0500
Subject: Re: Regional newspapers

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While I appreciate the pride you have in the birth of your
family, this is an inappropriate use of the Microscopy Listserver.
DO NOT do this again!!!!!


Nestor Zaluzec
SysOp






From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Tue, 10 Dec 1996 09:14:23 -0500
Subject: RE: zip drive

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Message-Id: {c=US%a=_%p=SYLVANIA%l=RD_EXC1-961210141423Z-9853-at-da-exc1.sylvania.com}

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} Sir,
}
} You might get some help from Iomega's support site:
}
} http://www.iomega.com/support/techs/zip/1.html#unix
}
}
} -------------------------------------------------
} Harold J. Crossman
} OSRAM SYLVANIA INC.
} Lighting Research Center
} 71 Cherry Hill Dr.
} Beverly, MA 01915
} Phone: (508) 750-1717
} E-mail: crossman-at-rd.sylvania.com
}
} Our web sites: www.sylvania.com
} www.siemens.com
} --
}
} "Crossman, Harold" {crossman-at-RD.SYLVANIA.com





From: Rehfeld, Cheryl :      CREHFELD-at-msmail.path.tch.tmc.edu
Date: Tue, 10 Dec 1996 08:27:00 -0600
Subject: subscribe

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Please add my name, Cheryl Rehfeld, to your list of subscribers. Thanks.




From: Vladimir Dusevich :      dusevich-at-astro.ocis.temple.edu
Date: Tue, 10 Dec 1996 11:06:12 -0500 (EST)
Subject: Re: Regional newspapers

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It's not interesting for me. Please be polite and never post such messages.

On Tue, 10 Dec 1996, Michel Warnau wrote:

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}
} Dear colleagues, Dear friends,
} We (Ali Temara and Michel Warnau) became new young fathers in the
} course of November 1996. We'd like first to announce it to the world as
} we are incredibly proud of our sons (Rayan and Nathan, respectively).
} Then we would like to ask to all of our colleagues and friends around
} the world to try to find the newspaper that was published on the day of
} their birth (November 17th and November 27th). Indeed, it is likely that
} you and/or a member of your family or a colleague keep newspapers without
} any particular aim and throw them away once a month. We would especially
} appreciate the front page of your regional newspapers.
} We take the opportunity of this message to wish you a Merry Christmas
} and a happy and prosperous New Year!
} Kindest regards,
}
} Ali & Michel
}
} ________________________________________________________________
} Dr. Ali Temara and Dr. Michel Warnau
}
} Laboratoire de Biologie Marine (CP 160/15)
} av. F.D. Roosevelt 50
} B-1050 Brussels (BELGIUM)
}
} Phone: 32/2/650 29 70 - 32/2/650 22 34 Fax: 32/2/650 27 96
} e-mail: atemara-at-ulb.ac.be
} mwarnau-at-ulb.ac.be
}




From: Hans Sluiman :      RBG_3/HANS
Date: Tue, 10 Dec 1996 12:13:42 BST
Subject: Video microscopy with Quadra 840AV

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Forwarded message:


I have a Macintosh Quadra 840AV which seems quite suitable for recording and
editing video movies as it comes with an S-video input port. What I'm
interested in is recording fairly slow processes such as cell division
in microscopic algae using a video camera attached to a microscope, capturing an
image, say every minute or so. Has anyone used an 840AV (or any other Mac) for
this purpose and what additional software (apart from the built-in Video Monitor
and FusionRecorder software) would be needed, if any, to do the job properly?

Hans Sluiman
Royal Botanic Garden Edinburgh
h.sluiman-at-rbge.org.uk





From: Microscopy-at-sparc5.microscopy.c
Date: 12/9/96 1:12 PM
Subject: Re: EM fields

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RE} } EM fields 12/10/96

Very informative, thank-you for the info. I have some serious field problems
around a 6300FE JEOL. I've sent copies of your reply to the facilities people
here. All attempts to locate the problem thus far have been futile. Your
suggestions are new to us and we will try them.

**********************************************************
Jake Schaper
Product Analysis Lab
Advanced Digital Consumer Division
Motorola, Inc.
Chandler, Az.
**********************************************************

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In resonse to the most recent discussion on magnetic fields, I thought I
might
be able to add some insight from the experiences we have had minimizing them
in
our facility. As a little background, our facility was built about 5 years ago

and was "designed" specifically to house our electron microscopes. From the
beginning we had magnetic field problems in some of our microscope rooms (but
not others), with field strengths as high as 3-4 mG at a couple of the
microscopes. In the service corridor that runs behind the microscope rooms
(power supplies, water chillers, elecrtrical breaker boxes, etc. are located
there) field strengths greater than 20 mG were measured near some of the
breaker
boxes. These fields did not go away even if all of the breakers were tripped.
They may have decreased somewhat but they certainly were not reduced to
acceptable levels. In fact, tripping the switches at the main distribution
panel
to cut off the power to our entire facility simultaneously did not cause the
fields to go away. We muddled around with several attempted fixes such as
having
electricians replace sections of electrical conduit with plastic tubing and
putting rubber insulators around the conduit where it was mounted into the
walls
or ceilings. We had various degrees of success with these "fixes" and some may

have made things worse.
After meeting directly with an outside consultant we were able to get a
good
understanding of what was necessary to eliminate the fields. Our problems were

entirely due to there being paths to ground other than the ground wires
themselves so that the ground did not run back to the electrical box. When
this
happens there is a net electrical current on the conduit and wires. It is this

net current that gives rise to the electromagnetic fields. If I remember
correctly the magnetic field associated with this current drops off as a
function of 1/r. This is compared to the fields originating from electronic
equipment that drop off as a function of 1/(r^3). I think you will also see
this
1/r cubed response if you measure the field from a power cord that has no net
current on it.
In my opinion the most important tool for tracking down the sources of the
fields is an AC current probe. A number of different models are available that

plug into a digital multimeter. Ours cost about $120 from Grainger. It works
by
simply clamping it around the wire or conduit you want to check. If the
current
readout is not zero you have a ground path somewhere that needs to be
eliminated. A magnetic field meter is also useful for general monitoring of
field strengths and tracking down sources behind walls and other inaccessible
places. Our meter is a Holaday Industries HI-3624A that we paid under $500 for

from Holaday Industries, Eden Prairie, MN ph.612-934-4920 (no financial
interest, just a satisfied user of a product from a local company).
In the end we were able to eliminate almost all of our electromagnetic
field
sources and the readings at the scopes are down to about 0.2 mG. If I were
planning a new facility I would insist that isolated ground recepticles and
switches be used everywhere. In the typical recepticle there is a place to
attach a ground wire but it is not electrically isolated from the mounting
yoke
of the recepticle. So, in our case where the recepticles were mounted into a
metal wire mold that in turn was screwed into the metal studs used for framing

the rooms we ended up with all kinds of possible ground paths. In this
instance
we were able to isolate the wire mold from the framing using nylon washers
around all of the mounting screws, but could have had to replace all of our
recepticles (at much greater expense). The wiring to the light fixtures was
also
a major field source. In this instance, our fixtures themselves could not be
isolated so the entire conduit run back to the electrical box had to be
isolated
from possible alternative ground paths. This is where the current probe was
most
useful. By checking the conduit on both sides of the possible grounding points

it could be quickly determined if isolation was necessary, and if so rubber
bushings could be installed. In regard to the observance of fields when power
was "disconnected," tripping the switch simply breaks the hot wire and a
current
can still flow along the neutral wire to the unintended grounding points. When

we first observed this we thought the field source may have been external to
our
electrical system and would require active EM field cancellation systems
($$$).
We do still have a number of very of minor field sources, but they haven't
been
worth the effort to eliminate.
For all you who have also had to deal with EM fields I hope this was of a
little help.
Mike

Mike Bench
Characterization Facility
Center for Interfacial Engineering
University of Minnesota
Voice: (612) 624-6590
Fax: (612) 626-7530
e-mail: bench-at-cems.umn.edu


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From: Mike Bench :      bench-at-cems.umn.edu
Date: Mon, 9 Dec 1996 12:58:22 -0600
Subject: Re: EM fields

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From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: Tue, 10 Dec 1996 09:20:52 -0800
Subject: Re: Regional newspapers

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boxjx-at-usthk.ust.hk, root-at-tinro.marine.su, christr-at-delm.tas.gov.au,
sav-at-aster.zin.ras.spb.ru, mona-at-rmit.edu.au, ph20+-at-andrew.cmu.edu,
laabir-at-sb-roscoff.fr, lnaegel-at-cibnor.conacyt.mx,
Microscopy-at-Sparc5.Microscopy.Com, emilien_pelletier-at-UQAR.UQUEBEC.CA,
alerkt-at-qo.fcen.uba.ar, itnhlib-at-scut.edu.cn, p.willemsen-at-kribc.tno.nl,
unabia-at-hawaii.edu, gmartine-at-socompa.cecun.ucn.cl, zocm-at-hippo.ru.ac.za,
hadfield-at-hawaii.edu, edupre-at-socompa.cecun.ucn.cl, mjangoux-at-ulb.ac.be,
Michel Warnau {mwarnau-at-ulb.ac.be}
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From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: 12/10/96 10:22 AM
Subject: Regional newspapers

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Ali & Michel;

While we all appreciate your excitement regarding the arrival of your new
family members, perhaps we should remember the goal of this listserver,
which is to disseminate information on microscopy-related topics. Thanks
for your consideration.

(BTW, congratulations)

*******************************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA 91711
USA
email: Bob_Citron-at-cc.chiron.com
*******************************************


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Dear colleagues, Dear friends,
We (Ali Temara and Michel Warnau) became new young fathers in the
course of November 1996. We'd like first to announce it to the world as
we are incredibly proud of our sons (Rayan and Nathan, respectively).
Then we would like to ask to all of our colleagues and friends around
the world to try to find the newspaper that was published on the day of
their birth (November 17th and November 27th). Indeed, it is likely that
you and/or a member of your family or a colleague keep newspapers without
any particular aim and throw them away once a month. We would especially
appreciate the front page of your regional newspapers.
We take the opportunity of this message to wish you a Merry Christmas
and a happy and prosperous New Year!
Kindest regards,

Ali & Michel

________________________________________________________________
Dr. Ali Temara and Dr. Michel Warnau

Laboratoire de Biologie Marine (CP 160/15)
av. F.D. Roosevelt 50
B-1050 Brussels (BELGIUM)

Phone: 32/2/650 29 70 - 32/2/650 22 34 Fax: 32/2/650 27 96
e-mail: atemara-at-ulb.ac.be
mwarnau-at-ulb.ac.be





From: psizrk-at-biruni.erum.com.pk
Date: Wed, 11 Dec 96 00:16 PST
Subject: Thanks (Jeol's TEM Problem]

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Dear all,

Thanks to everyone who has replied to my question about Jeol's TEM Problem. We
now have a lot of different things to try and I hope one will work.

Thank-you
Suzan





From: Olivier Schueller :      oschueller-at-gmwgroup.harvard.edu
Date: 10 Dec 1996 16:06:19 -0500
Subject: carbon TEM grids

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Hello,

I looking for information about carbon TEM grids.
I would like to find out the following:

typical mesh size
fabrication process
manufacturer
applications

Anything you can tell me, or any reference, would be quite useful. =
Thanks in advance.
Please respond to the following email address:
oschueller-at-gmwgroup.harvard.edu

Olivier




From: Dr. Mark W. Lund :      lundm-at-physc2.byu.edu
Date: Tue, 10 Dec 1996 15:10:15 MST/MDT
Subject: RE: zip drive

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Professor Mannheimer wrote:
} Hello fellow microscopists,
} Does anyone have experience in running an Iomega ZIP drive under
} Novell 7 DOS on a network?
} My computer Pentium 100, Windows 3.1 hangs when running the guest
} driver;
} if I boot on MS DOS 6.2 the drive runs fine.
} Have not been able to get help from Iomega on this.

We at MOXTEK have been enthusiastic about Novell Dos for a long
time, so I asked around. They say that Zip drives don't work
with Novel Dos 7 because the emm386 is not working right. If
you REM out the emm386 load in config.sys it should then work
fine.

best regards
mark

Mark W. Lund, PhD
Director } } Soft X-ray Web page http://www.moxtek.com { {
MOXTEK, Inc. *************************************************
Orem UT 84057 **"Soft x-rays in the 21st Century" conference **
801-225-0930 ** 8-11 January 1997, Midway Utah **
FAX 801-221-1121 ** http://volta.byu.edu/xray/info.html **
lundm-at-xray.byu.edu *************************************************

"Let me commend a great truth to you which has been one of the supports
of my life: 'The Gods send threads for a web begun.' Andrew Carnegie






From: lizard-at-okway.okstate.edu (Ginger Baker)
Date: Tue, 10 Dec 1996 16:24:25 -0800
Subject: SEM Filter Samples

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Hello,

I have an environmental engineer who is interested at looking at
bacterial samples that have been filtered. He wants to look at the
bacteria on the filters themselves. Does anyone know how to process
such samples? Do I let the filters air-dry or should I fix,
dehydrate, and CPD the filters? Any help would be much appreciated.

Thank you in advance,

Ginger Baker
EM Lab Manager
Dept. APP
250 Veterinary Medicine
Oklahoma State University
Stillwater, OK 74078
(405) 744-6765
FAX: (405) 744-5275
Email: lizard-at-okway.okstate.edu




From: Donald P. Cox :      goldmrkr-at-fast.net
Date: Tue, 10 Dec 96 18:03 EST
Subject: Acrylate Allergies!

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Dear Colleagues:

One of our customers has had a rather strong allergic reaction during the
handling and use of two embedding media simultaneously. One of the products
was Unicryl and the other, Technovet. I am very concerned about the
prevalence of such allergic reactions with acrylates, and am presently
attempting to gather all pertinent literature and/or subscriber experiences
with acrylate allergies.

A literature search on MEDLINE has revealed only one paper describing
contact dermatitis reaction to Lowicryl, but many papers concerning the
allergies to methacrylates used as adhesives by the dental profession and
for cosmetic purposes.

I would appreciate learning of any experiences of the members and to receive
any pertinent references regarding allergies to acrylate embedding media.

Regards, Don Cox
********************************************************
Donald P. Cox, Ph.D., M.B.A.
GOLDMARK BIOLOGICALS/D.P. COX ASSOCIATES
437 Lock Street, Phillipsburg, NJ 08865-2764
(908) 859-2631 - - (908) 859-2875-FAX
E-Mail: goldmrkr-at-fast.net/goldmarker-at-aol.com
Web Page: http://members.aol.com/goldmarker

~~~"Goldmarking is everlasting probing!"~~~
********************************************************






From: W.Jablonski-at-csl.utas.edu.au (Wis Jablonski)
Date: Wed, 11 Dec 1996 12:06:50 +1000
Subject: Re: SEM Filter Samples

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From: adam vivian-smith :      Adam.Vivian-Smith-at-adl.hort.csiro.au
Date: Wed, 11 Dec 1996 11:57:27 +1030
Subject: Re: Technovet/Technovit embedding resin

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} Dear Colleagues:
{SNIP}
} One of our customers has had a rather strong allergic reaction during the
} handling and use of two embedding media simultaneously. One of the products
} was Unicryl and the other, Technovet. I am very concerned about the
} prevalence of such allergic reactions with acrylates, and am presently
} attempting to gather all pertinent literature and/or subscriber experiences
} with acrylate allergies.
{SNIP}

Hi! Aside from the allergies - is it possible to request for the address of
the supplier of technovit/technovet. I have heard that technovit resin gives
excellent embedding for plant tissues, and I am wondering if anybody has
experience with fixation and embedding plant tissues in technovit? Could you
email me with some details please....thanks in advance, Adam.
''~``
( o o )
_____________________.oooO--(_)--Oooo._______________________________________
Adam Vivian-Smith
PhD Student
University of Adelaide/CSIRO Voice: +61 08 8303 8627
Division of Horticulture Fax : +61 08 8303 8601
Urrbrae, Adelaide Email: Adam.Vivian-Smith-at-adl.hort.csiro.au
S.A., 5064
AUSTRALIA
.oooO
( ) Oooo.
________________________\ (____( )_________________________________________
\_) ) /
(_/





From: Rantg-at-aol.com
Date: Tue, 10 Dec 1996 21:15:07 -0500
Subject: microscopy for hair electrolysis

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I am a professional electrologist, permanent hair removal specialist. I am
trying to find a way to determine the effect of high frequency current [heat]
and galvanic action [alkali ] on pre- keratinized cells of the hair follicle.
These currents are delivered to the lower part of the hair follicle via micro
needle, the hair is then extracted by forceps. The lower part of the hair
shaft contains matrix cells that are undifferentiated and capable of mitosis.
These cells are available for analysis. Are there any microscopic
techniques and \ or stains that will differentiate denaturated cells from
ones in the normal state?




From: Glenn Holm :      KARUZIS-at-wccf.mit.edu
Date: Tue, 10 Dec 1996 22:29:22 -0500 (EST)
Subject: What does OCT stand for?

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Somebody asked. The stuff one uses for cryostat cutting. What does OCT stand
for?




From: rw9-at-psu.edu (Rosemary A. Walsh)
Date: Tue, 10 Dec 1996 23:31:29 -0500
Subject: Re: SEM Filter Samples

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At 4:24 PM 12/10/96 -0800, Ginger Baker wrote:
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Ginger,
Once you filter the suspension of bacteria
through a 0.2um polycarbonate filter, remove
the filter from the housing and place it into
fixative (2.5% glutaraldehyde in a buffer)}
Follow this with:
buffer washes,
an optional post-fixation (osmium tetroxide)
buffer washes
gradient alcohol series (3X100% ETOH)
At this point you have 2 options;
3 washes in HMDS (hexamethyldisilizane)
followed by air-drying
or Critical Point Drying.

Fix the filters to the SEM stub with double-sided
sticky tape, add a drop of silver dag to the edge of
the filter and sputter-coat.

Additional tips---during the intial stage of filtering,
remove the syringe from the filter housing, pull
plunger back, reattach and push a column of air
through the housing---this pushes all of the filtrate
through the filter so that it doesn't drain off when
removing the filter from the Swinney holder.
-------------------the filter housing and filter can be
autoclaved ahead of time and sterile 1 cc. syringes
used to insure a reliable prep.
Best of luck,
Rosemary Walsh






From: marilyn-at-cemmsa.adelaide.edu.au (Marilyn Henderson)
Date: Wed, 11 Dec 1996 14:33:02 +0900
Subject: Re: What does OCT stand for?

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Optimum Cutting Temperature






From: Jouko =?iso-8859-1?Q?M=E4ki?= :      jouko.maki-at-utu.fi
Date: Wed, 11 Dec 1996 07:46:32 +0200
Subject: Re: SEM Filter Samples

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At 16:24 10.12.1996 -0800, you wrote:
Hi Ginger,
=20
} I have an environmental engineer who is interested at looking at=20
} bacterial samples that have been filtered. He wants to look at the=20
} bacteria on the filters themselves. Does anyone know how to process=
=20
} such samples? Do I let the filters air-dry or should I fix,=20
} dehydrate, and CPD the filters? Any help would be much appreciated.

I would definitely recommend to dehydrate and CPD the filters with
the bacteria. After they are dry, you can cut the filter for suitable size
to be mounted on a SEM-stud, whereafter they have to be coated for=
conductivity.
Good luck and happy holidays

Jouko

Jouko K. M=E4ki, Ph.D., Laboratory Manager
University of Turku, Laboratory of Electron Microscopy
Kiinamyllynkatu 10 FIN-20520 TURKU FINLAND
Tel: + 358 2 333 7318 GSM: + 358 40 505 2521 FAX: + 358 2 333 7380





From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Wed, 11 Dec 1996 00:12:57 -0500
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G'day all

Can I make a request to all those of you reply to questions.
Take a moment to edit out extraneous text from your reply
(yes I know that I'm responsible for some of that text aka
the new "banner"). But it is certainly true that you do not
have to "echo" or repost the entire message that you are
replying to. Cut/Edit out the irrelevant parts and send only
enough of the text so that the reader can get the gist of
what you are replying to. This little courtesy will
certainly make everyone's mail boxes a bit less cluttered
and also save space in the archives.

Thanks to all in advance

Nestor
Your Friendly Neighborhood SysOp







From: SveEn-at-pai.liu.se (Sverker Enestrom)
Date: Wed, 11 Dec 1996 08:07:53 +0100
Subject: Re: What does OCT stand for?

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OCT stands for Optimal Control Temperature fluid (polyvinyl alcohol and
polyethylene glycol) and is an embedding medium for frozen tissue
specimens.

*********************************************************
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
Fax: +46 13 13 22 57
*********************************************************






From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Wed, 11 Dec 1996 08:17:38 +0000
Subject: Acrylate Allergies! -Reply

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Dear Don

My predecessor here had a lot of problems using watyer
soluble Durcupan resin. He manifested what we now realise
was contact dermatitis, with skin blemishing, cracking and
peeling. He was also sensitive to the cured blocks, at least
certainly to their dust (we used to cure in ice cube trays and
then cut specimens out with a hacksaw for mounting prior to
ultramicrotomy as routine).

With best wishes - Keith Ryan

++++++++++++++++++++++++++++++++++++++++++++++++++
Keith Ryan B.Sc., Ph.D., C.Biol., M.I.Biol.
Plymouth Marine Laboratory, Citadel Hill,
Plymouth, Devon PL1 2PB, England

Tel: ++44 1752 633294 (international)
01752 633294 (national)
Fax: ++44 1752 633102 (international)
01752 633102 (national)
e-mail: k.ryan-at-pml.ac.uk
PML web site: http://www.npm.ac.uk/pml
++++++++++++++++++++++++++++++++++++++++++++++++++




From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Wed, 11 Dec 1996 08:18:14 +0000
Subject: Re: SEM Filter Samples

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Microscopy & Analysis (US Edition Issue No. 21, November 1996) ran an
article you might be interested in - "Cryo-preparation of small or lightly
attached biological specimens" by Robinson et al.

Basically, the idea is a variation on cryo-fixation - this normally
involves plunging a specimen into liquid N2. In the case of specimens
similar to yours, the result is that all the particles of interest drop
off! However, if you simply fix the specimen on to a stub at room
temperature and then transfer it to the pre-cooled cryo-stage, freezing is
still relatively rapid. Obviously, you still loose internal specimen detail
because of ice crystal growth but a considerable amount of external detail
is successfully preserved. The authors present a number of good SEM images.
Whether the procedure will work successfully with specimens as small as
bacteria, I'm not sure.

If you want further details, contact Ken Robinson at KRO-at-pcmail.nerc-bas.ac.uk.

Regards,

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
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From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Wed, 11 Dec 1996 08:18:10 +0000
Subject: Re: carbon TEM grids

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Agar Scientific in the UK (fax +44-1279-815106) sell 75 mesh carbon
composite grids. I'm sure SPI sell something similar in the US - check
their web site. Both will be able to give you details of fabrication.

As to applications, I guess the two main applications would be for EDX
analysis, to reduce background counts, and where specimen preparation
procedures would bring the grid into contact with something that could
corrode material such as Cu.

You might also want to consider carbon-coated nylon grids (but watch for Ti
contamination), Be, Ti and Al, depending on your application.

(I have no commercial interest in Agar, SPI, or other consumables suppliers)

Regards,

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom
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From: Microscopy
Date: 11 December 1996 01:43
Subject: Acrylate Allergies!

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Don

have you got this reference? It seems to be a key one referring to several
others:

Tobler, M. and Freiburghaus (1990); Occupational risks of (meth)acrylate
compounds in embedding media for electron microscopy
Journal of Microscopy 160: 291-298

Good luck in your search.

Malcolm Haswell
University of Sunderland
UK
e-mail es0mhs-at-environment.sunderland.ac.uk

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Dear Colleagues:

One of our customers has had a rather strong allergic reaction during the
handling and use of two embedding media simultaneously. One of the products
was Unicryl and the other, Technovet. I am very concerned about the
prevalence of such allergic reactions with acrylates, and am presently
attempting to gather all pertinent literature and/or subscriber experiences
with acrylate allergies.

A literature search on MEDLINE has revealed only one paper describing
contact dermatitis reaction to Lowicryl, but many papers concerning the
allergies to methacrylates used as adhesives by the dental profession and
for cosmetic purposes.

I would appreciate learning of any experiences of the members and to receive
any pertinent references regarding allergies to acrylate embedding media.

Regards, Don Cox
********************************************************
Donald P. Cox, Ph.D., M.B.A.
GOLDMARK BIOLOGICALS/D.P. COX ASSOCIATES
437 Lock Street, Phillipsburg, NJ 08865-2764
(908) 859-2631 - - (908) 859-2875-FAX
E-Mail: goldmrkr-at-fast.net/goldmarker-at-aol.com
Web Page: http://members.aol.com/goldmarker

~~~"Goldmarking is everlasting probing!"~~~
********************************************************






From: lag-at-pegasus.cc.ucf.edu (Lucille A. Giannuzzi)
Date: Wed, 11 Dec 1996 07:39:39 -0500
Subject: TEM phosphor plates vs. CCD camera systems

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What are the advantages/disadvantages (including price) in using the
reusable TEM phosphor plates vs. a dedicated CCD camera system for
collecting images?

Thanks.

**************************************************************************
Lucille A. Giannuzzi, Ph.D. phone: 407 823-5770
University of Central Florida fax: 407 823-0208
Dept. of Mechanical, Materials, and Aerospace Engineering
PO Box 162450
4000 Central Florida Blvd.
Orlando, FL 32816-2450 email: lag-at-pegasus.cc.ucf.edu
**************************************************************************






From: lamiller-at-ux1.cso.uiuc.edu (Lou Ann Miller)
Date: Wed, 11 Dec 1996 07:15:02 -0600
Subject: Re: Acrylate Allergies!

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Don,
I don't know what to tell you except that this allergy is common, I am
severly allergic to Lowicryl, and have been told that if I'm exposed to it
again on my hands, I may lose the use of my hands. It took me 6 months to
get the feeling back in my finger tips, and my fingers were so bad they
were purple black. Also, the fumes from methacrylates etc is of no help to
my chronic asthma.

I usually run into someone with the same problem in the Histology / EM
field where ever I go.

We ordered the only gloves we found impermiable, H-4 Gloves from Monsanto,
but they are very ackward to work in.

Lou Ann


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***************************
Lou Ann Miller
Service Supervisor
Center for Microscopy and Imaging
College of Vet. Medicine
University of Illinois
2001 S Lincoln Ave
Urbana,Illinois 61801
217-244-1566
lamiller-at-ux1.cso.uiuc.edu

Microscopy Home Page:
http://www.cvm.uiuc.edu/MicImagLab/MicImagLab.html

Central States Microscopy Society
http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/CSMS/csms.html

Personal Home Page:
http://www.cvm.uiuc.edu/HomePages/LouAnnMiller/LAM.html






From: rjpalmer-at-utkux1.utk.edu (Robert J. Palmer Jr.)
Date: Wed, 11 Dec 1996 08:51:48 -0400
Subject: Re: SEM Filter Samples

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You presumably have a bacteriology group in your vet school. Ask them
about acridine orange or DAPI as stains for bacteria on memebrane filters.
Rob Palmer
CEB/UT





From: Greg Erdos :      gwe-at-biotech.ufl.edu
Date: Wed, 11 Dec 1996 08:58:00 -0500
Subject: Re: SEM Filter Samples

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I would try to minimize handling as much as possible to avoid losing any
bacteria. We have resorted to exposure to osmium vapors for an extended
period such as 24-72 hours and then air drying.
At 04:24 PM 12/10/96 -0800, you wrote:

} Hello,
}
} I have an environmental engineer who is interested at looking at
} bacterial samples that have been filtered. He wants to look at the
} bacteria on the filters themselves.
*******************************************************
G.W. Erdos, Ph.D. Phone: 352-392-1295
Scientific Director,
ICBR Electron Microscopy Core Lab
218 Carr Hall Fax: 352-846-0251
University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~emcl/

*****
"Many shall run to and fro, and knowledge shall be increased"
Daniel 12:4





From: pdf-at-fullam.com
Date: Wed, 11 Dec 1996 08:58:41 -0500 (EST)
Subject: Re: carbon TEM grids

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We can furnish the following:
No. 25510: Carbon Polymer Composite Grid by CRI, 75 mesh. Low x-ray
background. $6.75 each

No. 25500: EFFA Carbon Coated Nylon Grid, 180 mesh, Coated heavily on both
sides with carbon. Low x-ray background. Also good for backscattered
imaging. $5.00 for vial/25

see page 38 in 1992/3 catalog

We also sell thin-film substrates
No. 11250 Carbon Films on 200 mesh copper grids $2.90 each; vial/100 $260
Special films are also made to order

We also have No. 14570: Carbon Mesh
A fine lacy carbon film for use as a substrate in cases where a continuous
solid substrate background would obscure the specimen. These come on 200 or
300 mesh copper grids.


Dianne


}
} Hello,
}
} I looking for information about carbon TEM grids.
} I would like to find out the following:
}
} typical mesh size
} fabrication process
} manufacturer
} applications
}
} Anything you can tell me, or any reference, would be quite useful. Thanks
in advance.
} Please respond to the following email address:
} oschueller-at-gmwgroup.harvard.edu
}
} Olivier
}
Ernest F. Fullam, Inc.
Phone: (518) 785-5533 FAX: (518) 785-8647
E-Mail: pdf-at-fullam.com

************************************************************
* Complete on-line product listing: http://www.fullam.com/ *
************************************************************





From: rossetto-at-obelix.unicamp.br (Estela S. Rossetto)
Date: Wed, 11 Dec 1996 12:34:38 -0200 (BDB)
Subject: Technovit resin/plant material

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Hi all,
I am also interested in the informations asked by Adam, about the
use of Technovit resin for plant material. Would you please send them also
for me?
Could someone help me in another question? How do you find a good
protocol for freeze-substitution of plant material (hydrated and desiccated
leaves)? I have tryed three substitution solutions and some
substitution-times between 2 and 12 weeks, but the results were good for
just one species, not for the other two.
What do you use as substitution-solution? And for subst-time?
Thank you in advance!
Estela
____________________________________________________

Estela S. Rossetto
Ph.D. Student
Departamento de Biologia Celular
Instituto de Biologia - Unicamp
Universidade Estadual de Campinas
C.P. 6109
13081-970 Campinas SP
Brasil
Tel:(019) 2397821 Fax:(019) 2393124
e-mail: rossetto-at-obelix.unicamp.br
____________________________________________________





From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Wed, 11 Dec 1996 07:11:05 -0800 (PST)
Subject: Re: What does OCT stand for?

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Hi,
I think it stands for "Optimum cutting temperature". At lease that is
what I was told.

Bob Underwood
Morph Core Lab
U of Washington

On Tue, 10 Dec 1996, Glenn Holm wrote:

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} Somebody asked. The stuff one uses for cryostat cutting. What does OCT stand
} for?
}





From: kna101-at-utdallas.edu
Date: Wed, 11 Dec 1996 09:07:50 -0600 (CST)
Subject: Re: Acrylate Allergies!

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Dear Don,

I have personal experience with methyl methacrylate contact
allergies. Years ago, when I was taught how to embed and section JB-4
(from Polyscienses), I was told that the solutions could cause a poison
ivy-like reaction if I got it on my skin. My teacher, however chose not
to were gloves, so neither did I. After a couple of months of continuous
use, I developed the worst reaction that you could imagine. The fingers
that had been in contact with the liquid form of the acrylic started to
itch and burn, deep inside the flesh. Next, my fingers swelled to the
size of a meaty hotdog and itched like crazy. Any attempt to even touch
then sent sharp pains through my hand. I took antihistamines as soon as
the reaction started and applied ointment for two days before the reaction
was over. After that episode, I accidently touched the solid form once
or twice and scrubbed my hands immediately and at length And took some
more antihistamines. Still, I got a minor reaction that lasted a day
each time.
Now, about 10 years later, I still use JB-4 for alot of our
samples, but I Always were gloves, and glasses when I'm arround it and I
always wipe
down work surfaces when I'm finished for the day. I have not had another
even minor reaction for at least the last 5 years and never one as bad as
the first.
The reactions to these acrylics can be quite painfull, but
many of the other embedding medias available are carcinogenic, with no
early warnings. At least
with this material, I know immediately when I've gotten too careless with my
safety precautions.

Karen Pawlowski

On Tue, 10 Dec 1996, Donald P. Cox wrote:

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} -------------------------------------------------------------------------.
}
} Dear Colleagues:
}
} One of our customers has had a rather strong allergic reaction during the
} handling and use of two embedding media simultaneously. One of the products
} was Unicryl and the other, Technovet. I am very concerned about the
} prevalence of such allergic reactions with acrylates, and am presently
} attempting to gather all pertinent literature and/or subscriber experiences
} with acrylate allergies.
}
} A literature search on MEDLINE has revealed only one paper describing
} contact dermatitis reaction to Lowicryl, but many papers concerning the
} allergies to methacrylates used as adhesives by the dental profession and
} for cosmetic purposes.
}
} I would appreciate learning of any experiences of the members and to receive
} any pertinent references regarding allergies to acrylate embedding media.
}
} Regards, Don Cox
} ********************************************************
} Donald P. Cox, Ph.D., M.B.A.
} GOLDMARK BIOLOGICALS/D.P. COX ASSOCIATES
} 437 Lock Street, Phillipsburg, NJ 08865-2764
} (908) 859-2631 - - (908) 859-2875-FAX
} E-Mail: goldmrkr-at-fast.net/goldmarker-at-aol.com
} Web Page: http://members.aol.com/goldmarker
}
} ~~~"Goldmarking is everlasting probing!"~~~
} ********************************************************
}
}
}




From: MAIL-11 Daemon
Date: Mon, 09 Dec 1996 17:19:34 -0500 (CDT)
Subject: Re: High resolution printer -Reply

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From: MAIL-11 Daemon
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I sent you a message just few minutes ago asking you to resend your
message. I misquoted the version of my WordPerfect. It is a WP 6.1 not 7.
Sorry.

Ann Fook

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From: MAIL-11 Daemon
Date: Mon, 09 Dec 1996 15:41:30 -0500 (CDT)
Subject: Microscopist position

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Please post the following advertisement for an academic position at The
University of Calgary for a Structural Biologist/Microscopist.



STRUCTURAL BIOLOGIST


The University of Calgary Department of Anatomy invites applications for a
fulltime academic position as a structural/cell biologist. This position offerss
an excellent opportunity for independent and collaborative research with
other structural biologists employing technologies such as magnetic
resonance spectroscopy and x-ray diffraction in the university-wide structural
biology group, and for access to the University s Microscopy and Imaging
Facility, comprising state-of-the-art electron and computer-based light
microscopies. Duties will also include undergraduate teaching and graduate
student supervision.

Qualifications include a PhD or equivalent, at least two years of
postdoctoral experience, and a proven record of excellence in a research
program which includes the development of advanced imaging techniques.
Researchers particularly encouraged to apply are those with interests at the
cellular or molecular level in an area complementing activities of a Faculty
of Medicine research group such as Cancer Biology, Molecular &
Developmental Biology, Joint Injury & Arthritis, etc. More information is
available at http:/www.ucalgary.ca/~resoff/index.html.

The successful candidate must compete successfully for salary and
establishment grant support from the Alberta Heritage Foundation for
Medical Research and/or the Medical Research Council, and will have 75%
of time protected for research.

In accordance with Canadian immigration requirements, priority will be
given to Canadian citizens and permanent residents of Canada. The
University of Calgary is committed to Employment Equity.

Please submit a curriculum vitae and statement of research and goals, and
arrange for three letters of reference to be sent directly, by January 30,
1997, to:


Dr. D. Bazett-Jones
Chair, Search Committee
Department of Anatomy
3330 Hospital Drive
Calgary, AB T2N 4N1, Canada
TEL: (403) 220-3025, FAX: (403) 270-0737

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From: DAVID PATTON :      D-PATTON-at-wpg.uwe.ac.uk
Date: Wed, 11 Dec 1996 14:14:17 +0000
Subject: SEM Filter Samples -Reply

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Some bacteria can stand airdrying. I suggest trying this with
part of the sample. If the bacteria or other organisms look
deflated you can CPD the remainder.

Dave





From: Brad Goodwin :      goodwib-at-wdni.com
Date: Wed, 11 Dec 1996 08:43:01 -0800
Subject: Thanks for info about LR White "ripples"

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I just want to thank everyone (19 replies) that sent me possible
solutions to the problem of "ripples" in LR White sections.

When and if the problem is solved, I will post the procedure.

Thanks again,




From: Susan Carbyn :      CarbynS-at-em.agr.ca
Date: Wed, 11 Dec 1996 11:24:44 -0500
Subject: Technovit resin/plant material

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Perhaps the technovit resin/plant material could be discussed on the
listserver, as I am interested in any information and perhaps others are
too.

Susan




From: DAVID PATTON :      D-PATTON-at-wpg.uwe.ac.uk
Date: Wed, 11 Dec 1996 15:31:31 +0000
Subject: Subject OK; Message Blank - Help

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From: edb-at-chem.psu.edu (Ed J. Basgall)
Date: Wed, 11 Dec 1996 11:49:19 -0500
Subject: Re: SEM Filter Samples

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Ginger:

I have done similar preps while at UMASS. Are the bacteria already
on the filters? There are several kinds of filters. Some are better
than others for SEM. The best ones to use for SEM are polycarbonate
(Nuclepore type, Bio-Rad also sold some). Other ones are
a torturous path type and it will be hard to find the bacteria on them.

Your best bet if there is a ?? is to look at an unused filter so you know
what the background is. Polycarbonate ones are smooth with round holes in them.
They come in several pore sizes. You will probably want ~.2um for bacteria.
Too many bacteria will clog them up and stop the flow. My guess is these are
some type of water filters. Certainly try to get a control to look at.

By all means fix the samples, osmicate, ethanol dehydrate, CPD and Au/Pd coat
for best results. Fixing should also aid in keeping the bacteria in place.
You can try air drying for comparison but the morphology will
be poor. It depends on what your final purpose is; bacterial counts or
being able to identify types and have nice morphology. I would recommend
running a test batch first to be sure there are no problems. Feel free
to call if you have any ???

Is Bill Chissoe at Stillwater?

Best of luck

Ed Basgall, PhD
Penn State Univ.
Dept. of Chemistry
University Park, PA 16802
Ph: 814-865-0493





From: Tom Phillips :      tphillips-at-biosci.mbp.missouri.edu
Date: Wed, 11 Dec 1996 11:17:43 -0500
Subject: Re: What does OCT stand for?

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I have seen several people propose that OCT stands for Optimal Cutting
Temperature. That doesn't seem to make sense inasmuch as OCT is used at
lots of different temperatures. My guess is that it stands for OPTIMAL
CUTTING TEXTURE.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(573)-882-4712 (voice)
(573)-882-0123 (fax)






From: Microbill-at-aol.com
Date: Wed, 11 Dec 1996 12:21:26 -0500
Subject: Re: TEM phosphor plates vs. CCD camera systems

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The phosphor plates have (potentially) a higher resolution than currently
affordabel CCD devices. Actually, at the last price (I haven't seen the
latest plate prices) I saw for the plate system you could probably afford a
2kx2k CCD camera. The camera is quicker but 2k is just bearly good enough if
you expect to use the images like you are accustomed to, i.e. like film. A 2k
image blown up to 8" is only 250 dpi - not very good. Even an average laser
printer will do 600 dpi. The images will be OK if printed with a dye sub
printer (300dpi) but you probably will not be able to enlarge sub areas of
the image and get what you would expect from film. Area array CCD's bigger
than 2k are still rare.

Bill Miller




From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Wed, 11 Dec 1996 11:34:37 -0600
Subject: collagen - TEM

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I need to fix, stain, embed and section experimental collagen gels (not animal
tissues) for TEM.

I'm looking for specific fixation, staining and dehydration advice. I thought
I'd try buffered glutaraldehye, buffered osmium tetroxide for starters. Any
advice as to concentrations, buffers, pH? For dehydrating, is either acetone or
ethanol preferred?

As for staining, apparently uranyl acetate stains collagen intensely (Hayat,
Positive Staining, p. 37). Should it be used after washing out fixatives before
beginning dehydration, during dehydtation (eg. 50% ethanol or acetone), or only
as a post-stain on sections?

Thanks for any help you can give.


Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"When the mode of the music changes, the walls of the city will shake." - PLATO
"There's a whole lotta shakin' goin' on!" - CHUCK BERRY





From: MARK DARUS (216) 266-2895 GENERAL ELECTRIC CO. :      darus-at-cle.dnet.ge.com
Date: Wed, 11 Dec 96 12:20:20 EST
Subject: S.G.

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Message-Id: {199612111720.MAA25003-at-thomas2.ge.com}


/\
/\ //\\
/\ //\\///\\\ /\
//\\ ///\////\\\\ /\ //\\
/\ / ^ \/^ ^/^ ^ ^ \/^ \/ ^ \
/ ^\ /\ / ^ / ^/ ^ ^ ^ ^\ ^/ ^^ \
/^ \ / ^\/ ^ ^ ^ / ^ ^ ^ \/ ^ ^ \ *
/ ^ ^ \/^ ^\ ^ ^ ^ ^ ^ ^ ____ ^ ^ \ /|\
/ ^ ^ ^ \ ^ _\___________________| |_____^ ^ \ /||o\
/ ^^ ^ ^ ^\ /______________________________\ ^ ^ \ /|o|||\
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/^ ^ ^ ^^ ^ ||___|___||||||||||||___|__||| /||o||||||\
/ ^ ^ ^ ^ ||___|___||||||||||||___|__||| | |
/ ^ ^ ^ ^ ^ ^ ||||||||||||||||||||||||||||||oooooooooo| |ooooooo
ooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo

_ __ __ __
/ ) ) ) / ) / /
/ / / _ o_ o_ _ / / o ---/---
/ / / /_) / ) / ) / ) / | /_ o_ / _ _ __
/ / \__(___/ (_/ (_/ (__/ | / ) / ) / /\ / / ) ) / | /\
___/_ \___/ (_/ (_/(_/__)_/(_/ / (_/\_/(_/__)
(__/
\|/ \|/ \|/
--O-- --O-- --O--
/|\ HAPPY /|\ HAPPY /|\
//|\\ //|\\ //|\\
///|\\\ NEW YEAR ///|\\\ NEW YEAR ///|\\\
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/////|\\\\\ !!! /////|\\\\\ !!! /////|\\\\\
0 0 ||| 0 0 0 0 ||| 0 0 0 0 ||| 0 0
__|||__ __|||__ __|||__





From: CM2-at-mail.polymtl.ca (CM2)
Date: Wed, 11 Dec 1996 15:23:13 -0500
Subject: Looking for a used High Performance DMA channel for PC

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We have in our lab a local computer network based on TokenRing. To
link our old Link AN10/PDP11 computer to that network, we use a DMA
channel. So this configuration works with a high performance
bi-directional 16 bit parallel DR11-W compatible DMA channel for IBM
PC-AT and compatible. The name of the board: XA-240 from
Electrograph.

Since two weeks this link is down and we believe that this is related
to the failure of the DMA board in the PC.

So we're looking for a used XA-240 board to evaluate if the problem is
related to that board or to the Link AN10. If possible we will
replace our board to repair that part of our network.

If anyone knows a way to get a board like this please let me know
ASAP!


Eric Baril
Centre for Characterization and Microscopy of Materials, (CM)2
Ecole Polytechnique de Montreal
2900, Edouard-Montpetit
Montreal (Quebec)
Canada
H3C 3A7
Tel. (514) 340-4788
Fax. (514) 340-4468

Eric.Baril-at-cm2.polymtl.ca








From: Blackwood, Andrew :      ablackwood-at-2spi.com
Date: Wed, 11 Dec 96 09:48:32 -0500
Subject: Carbon Grids for TEM

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Message-Id: {199612111900.OAA04365-at-ns1.axs2000.net}
To: "microscopy-at-Sparc5.Microscopy.com" {microscopy-at-Sparc5.Microscopy.Com}

-- [ From: Blackwood, Andrew * EMC.Ver #3.0 ] --

11 December 1996

Greetings:

Olivier Schueller asked about carbon grids. There are actually several
types available, including "aperture" grids, which have single, large holes
in the middle, 75 mesh pyrolytic carbon grids and carbon-coated nylon mesh
grids. It all depends on what you are trying to do.

All of these are described in detail on our web site.

Disclaimer, all of these products are available from my employer, Structure
Probe/SPI Supplies. Obviously, I have an interest in promoting their use.

Andy

Andrew W. Blackwood, Ph.D.
Structure Probe, Inc.
P.O. Box 656
West Chester, PA 19381-0656
Ph: 1 610 436 5400
FAX: 1 610 436 5755
e-mail: ablackwood-at-2spi.com
WWW: http://www/2spi.com






From: Blackwood, Andrew :      ablackwood-at-2spi.com
Date: Wed, 11 Dec 96 09:48:32 -0500
Subject: Carbon Grids for TEM

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-- [ From: Blackwood, Andrew * EMC.Ver #3.0 ] --

11 December 1996

Greetings:

Olivier Schueller asked about carbon grids. There are actually several
types available, including "aperture" grids, which have single, large holes
in the middle, 75 mesh pyrolytic carbon grids and carbon-coated nylon mesh
grids. It all depends on what you are trying to do.

All of these are described in detail on our web site.

Disclaimer, all of these products are available from my employer, Structure
Probe/SPI Supplies. Obviously, I have an interest in promoting their use.

Andy

Andrew W. Blackwood, Ph.D.
Structure Probe, Inc.
P.O. Box 656
West Chester, PA 19381-0656
Ph: 1 610 436 5400
FAX: 1 610 436 5755
e-mail: ablackwood-at-2spi.com
WWW: http://www/2spi.com






From: Karin Limburg :      CAKL-at-VM.MARIST.EDU
Date: Wed, 11 Dec 96 17:17:47 EST
Subject: On a quest for a microprobe standard

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Hi-

I have a need for an unusual standard to be used on a microprobe (WDS) for
quantitative trace element work. I need a carbonate with low quantities
(e.g., 0.5-2% by weight) of SrO. Preferably the standard would be homo-
geneous and the composition known accurately.

If anyone can point me in the right direction to track such a standard
down, I'd be very grateful.

Karin E. Limburg
Institute of Ecosystem Studies
Millbrook, NY 12545

p.s. the application for which this standard is desired is to measure trace
quantities of Sr in fish ear-stones.




From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Wed, 11 Dec 1996 16:54:48 -0500
Subject: Test from Nestor

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Sorry Folks, this is a system test. I've had reports of problems
at some sites.

Nestor






From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Wed, 11 Dec 1996 17:03:23 -0500
Subject: Please report problems to me directly

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Hi All

I think I have found the mysterious problem that has affected
some of you in the last 12 hours. At one point the new header
started printing a "." at the start of a line. Some Email systems
read this "dot" as end of message and then truncate the remaining
posting.

If you are still experiencing problems please Email me direct.
at

Zaluzec-at-Microscopy.Com

So I can keep track of the problem

Sorry,......
Nestor






From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Wed, 11 Dec 1996 18:26:36 -0600
Subject: Re: SEM Filter Samples

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} I have an environmental engineer who is interested at looking at
} bacterial samples that have been filtered. He wants to look at the
} bacteria on the filters themselves. Does anyone know how to process
} such samples? Do I let the filters air-dry or should I fix,
} dehydrate, and CPD the filters? Any help would be much appreciated.
}
} Thank you in advance,
}
} Ginger Baker

If the samples haven't already been filtered, then first sputter
coat the filters before use, or use silver filters. Fewer imaging problems
that way.
With the bacteria on the filters, fix, dehydrate, and dry from
hexamethyldisilizane at room temp or 60 C in a fume hood! I usually get
excellent results this way.
(Note: check Crang and Klomperens "Artefacts in Biological EM",
title approximate, about fixing and dehydrating and their effects on
bacterial coats. The slime coat may be important, and it's usually lost.)
Phil

&&& Illigitimi non carborundum &&&&&&&&
Philip Oshel
Microscopy
Station A
PO Box 5037
Champaign, IL 61825-5037
(217)244-3145 days
(217)355-3145 evenings
oshel-at-ux1.cso.uiuc.edu
*** looking for a job again ******************







From: micrskop-at-postoffice.ptd.net :      Mark.Moore-at-Sparc5.Microscopy.Com
Date: Wed, 11 Dec 1996 19:52:35 -0600
Subject: Free No-Obligation Help-Line for Microscope Repair and Troubleshooting

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Greetings from MICROSCOPY USA ( www.micro-usa.com)

- Free E-Mail Help-Line for Clinical Microscopy and Electronic Manufacturing.
( micrskop-at-postoffice.ptd.net )

- Video-Training Series w/ Troubleshooting Manual

- Wholesale Bulbs

- Service Center for Microscope Repair

- Individualized Training Programs for Biomedical and Laboratory Personnel
related to Microscope Repair and Troubleshooting.

E-mail or Fax (610) 327-1016, for More Information.







From: Blackwood, Andrew :      ablackwood-at-2spi.com
Date: Wed, 11 Dec 96 09:48:32 -0500
Subject: Carbon Grids for TEM

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Nestor,

I'm still receiving blank messages from the server. Please describe
your fix again. Thank you.

Phil

To: "microscopy-at-Sparc5.Microscopy.com" {microscopy-at-Sparc5.Microscopy.Com}




From: Neelima Shah :      shahn-at-mail.med.upenn.edu
Date: Wed, 11 Dec 1996 22:31:54 -0500
Subject: Re: collagen - TEM

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At 11:34 AM 12/11/96 -0600, you wrote:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

2.5% glut should be fine and 1% OsO4 is all you need. But for dehydration
using ethanol would be best.


} As for staining, apparently uranyl acetate stains collagen intensely (Hayat,
} Positive Staining, p. 37). Should it be used after washing out fixatives
before
} beginning dehydration, during dehydtation (eg. 50% ethanol or acetone), or
only
} as a post-stain on sections?

I think if you stain with 2%aq. UA before starting the dehydration(but
definately after throughly washing the buffer out) then there is very little
chance that it would stain intensly. alcoholic ua tends to stain darker.
This will give a chance to decide if post embedding staining is needed.


} Thanks for any help you can give.
}
}
} Gib Ahlstrand, MMS Newsletter Editor
} Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
} 495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
} 612-625-9728 FAX, giba-at-puccini.crl.umn.edu
}
} "When the mode of the music changes, the walls of the city will shake." - PLATO
} "There's a whole lotta shakin' goin' on!" - CHUCK BERRY
}
}
}
Regards...
:-) :-) :-) :-) :-) :-) :-) :-) :-) :-) :-) :-)
Visit us at http://www.med.upenn.edu/~path/core/EMCMAIN1.htm





From: W.M.Nichols :      GCP3198-at-KAAU.EDU.SA
Date: Thu, 12 Dec 96 07:55:12 G+3
Subject: Re: Regional newspapers

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Message-Id: {199612120452.WAA01627-at-Sparc5.Microscopy.Com}

PLEASE BE SUSPICIOUS ABOUT THE REGIONAL NEWSPAPER REQUEST
I smell the possibility of SPAM
W.Nichols




From: wa5ekh-at-juno.com (Charles J Day)
Date: Thu, 12 Dec 1996 01:38:48 EST
Subject: Re: Acrylate Allergies!

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i do not recall her name off hand, but if you contact rmc,inc
tucson,arizona,(they probably have a web page) and ask who was their
biological instructor at their ultra microtomy conference this year in
oct . I specifically remember, and i was surprised that she repeatedly
cautioned the group about similar severe allergy-proned
individual-reactions being rather common. I believe she also may be
linked to this newsgroup and may reply directly. i believe she was
retired and now consulting from her home in the Stanford area. when i can
get to my records i'll try to remember to recover her name and send her
e-mail address. i'm quite bad with names.
On Tue, 10 Dec 96 18:03 EST "Donald P. Cox" {goldmrkr-at-fast.net} writes:
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of
} America




From: Garber, Charles A. :      cgarber-at-2spi.com (by way of Nestor J. Zaluzec)
Date: Thu, 12 Dec 1996 01:23:04 -0500
Subject: Re: SEM Prep: Membrane filters

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} }
} } Ginger Baker wrote:
} } --------------------------------------------------------
} } I have an environmental engineer who is interested at looking at
} } bacterial samples that have been filtered. He wants to look at the
} } bacteria on the filters themselves. Does anyone know how to process
} } such samples? Do I let the filters air-dry or should I fix,
} } dehydrate, and CPD the filters? Any help would be much appreciated.
} } --------------------------------------------------------
} } These kinds of samples really do have to be critical point dried, if
} } examination is contemplated in a "conventional" SEM. In some instances,
} } using silver membranes makes it possible to get away with looking at the now
} } CPD's bacteria with far less or no metallization, an advantage in some
} } situations. More information about silver membrane filters can be found on
} } our website given below.
} }
} } Chuck
} }
} } =====================================================
} } Charles A. Garber, Ph. D.
} e-mail:cgarber-at-2spi.com
} } PRESIDENT
} } SPI SUPPLIES/STRUCTURE PROBE, INC.
} } WEST CHESTER, PA 19381-0656 USA
} }
} } Look us up at
} } xxxxxxxxxxxxxxxxx
} } http://www.2spi.com
} } xxxxxxxxxxxxxxxxx
} } ====================================================






From: LARNOULD J. :      larnould-at-mnet.fr
Date: Thu, 12 Dec 1996 09:10:57 +0100
Subject: High resolution printer

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Hi all,
I would like to thank all the people who answer to my question about
LazarPrinter system.
It was very helpfull.
I can attach the summarize of the answers to all interresting persons.(If
too much I'll directly mail on
the listserver).
Merry Xmas and happy new year to everybody.
==========================================================
Jacky Larnould
tel 33 (0)4 67 72 28 26
fax 33 (0)4 67 79 54 90
email larnould-at-mnet.fr





From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Thu, 12 Dec 96 04:18:20 -0500
Subject: Santovac 5

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Message-Id: {199612120904.EAA12698-at-ns1.axs2000.net}
To: MICROSCOPY BB {Microscopy-at-Sparc5.Microscopy.Com}

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

The following is a verbatum copy of part of a letter that was sent to SPI
Supplies by Monsanto, dated December 3, 1996:

Dear Cutomer:

Monsanto has made a decision to exit the plyphenyl ether business: OS-124,
OS-138 and Santovac 5 products by the end of the first quarter, 1997. We
are continuing to seek a buyer who would be capable of producing this
complex chemistry and will keep you informed of our progress. We want to
signal you now so you can minimize the disruption this transition may have
on your business.
------------------------------------------

The rest of the letter details how remaining stocks will be allocated to
their distributors.

The letter ends with the following statement: If we receive sufficient
requests for volume and we have not identified a suitable buyer for the
business, we will schedule manufacture during 1997.


As a long time distributor, we are sorry to see Monsanto leaving this
business. While the recent trend in EM has been away from diffusion pumps,
there are still a large number of EMs that run on Santovac 5.

The purpose of this posting is not to cause a stampeed to the order
departments but to make sure that there is adequate disclosure of this
information within the markets we (e.g. SPI) serve. We do not know any more
than what was stated in the December 3 letter. While we don't anticipate
that there would be a complete ceasation of the production of Santovac by
anyone, we do see the real possibility that there could indeed be a period
of shortages. Our best advice is to keep a spare bottle on the shelf.

Chuck


======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
=====================================================




From: Philip Koeck :      Philip.Koeck-at-csb.ki.se
Date: Thu, 12 Dec 1996 13:17:58 +0100
Subject: Re: Regional newspapers

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W.M.Nichols wrote:

} PLEASE BE SUSPICIOUS ABOUT THE REGIONAL NEWSPAPER REQUEST
} I smell the possibility of SPAM
} W.Nichols

What is SPAM?
--
Philip Koeck
Karolinska Institutet
Dept. of Bioscience
Novum
S-14157 Huddinge
Sweden
Tel.: +46-8-608 91 93
Fax.: +46-8-608 92 90
Email: Philip.Koeck-at-csb.ki.se




From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Thu, 12 Dec 1996 08:24:58 -0400 (AST)
Subject: Re: Regional newspapers

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X-Nupop-Charset: English

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In message Thu, 12 Dec 96 07:55:12 G+3,
"W.M.Nichols" {GCP3198-at-KAAU.EDU.SA} writes:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} PLEASE BE SUSPICIOUS ABOUT THE REGIONAL NEWSPAPER REQUEST
} I smell the possibility of SPAM
} W.Nichols
}
Yeah, that was the silliest thing I've ever seen on this listserver. But
what is SPAM? I mean besides the canned stuff.
F.C. Thomas
GSC (Atlantic) MicroAnalysis Facility
P.O. Box 1006, Dartmouth, N.S.
B2Y 4A2
(902) 426-4635




From: Spectra Services :      mspecht-at-frontiernet.net
Date: Thu, 12 Dec 1996 07:22:09 -0500
Subject: Re: High resolution printer

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At 09:10 AM 12/12/96 +0100, you wrote:
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Thanks

Michael

}
MIKE SPECHT
SPECTRA SERVICES, INC.
1653 East Main Street
Rochester, NY 14609
(716)654-9500
1800-955-7732
Fax: 716-654-8426
Visit our Web site at http://www.frontiernet.net/~mspecht/





From: Jim Darley :      p&s-at-ultra.net.au
Date: Fri, 13 Dec 1996 01:16:27 +1100
Subject: Re: On a quest for a microprobe standard

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At 17:17 11/12/96 EST, you wrote:
}
} I have a need for an unusual standard to be used on a microprobe (WDS) for
} quantitative trace element work. I need a carbonate with low quantities
} (e.g., 0.5-2% by weight) of SrO. Preferably the standard would be homo-
} geneous and the composition known accurately.
} Karin E. Limburg

} p.s. the application for which this standard is desired is to measure trace
} quantities of Sr in fish ear-stones.
*********************************
Aragonite, the lime stone from coral would qualify, e.g. it's a carbonate
and has small amounts of Sr. I do not know about it's homogeneity within one
sample.
Jim Darley
Probing & Structure (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site: http://www.ultra.net.au/~pns/





From: Peggy Brannigan :      brannign-at-asrr.arsusda.gov
Date: Thu, 12 Dec 1996 09:13:33 -0500
Subject: Re: High resolution printer

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Hi, I would be interested in the info on the high resolution printers. Thanks!

Peggy

Peggy Brannigan
Electron Microscopy
Floral and Nursery Plants Research Unit
National Arboretum

Bldg. 010A R.238
10300 Baltimore Avenue
Beltsville, MD USA20705

Phone: (301) 504-6097
Fax : (301) 504-5096
Email: brannign-at-asrr.arsusda. gov







From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 11 Dec 1996 20:36:58 -0400
Subject: RE-TEM tests

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Message-ID: {n1361738550.15162-at-mse.engin.umich.edu}

Subject: Time: 8:29 PM
OFFICE MEMO RE:TEM tests Date: 12/11/96

You might get a considerable amount of useful information out of Chapters 8 &
9 of the book "Experimental High Resolution Electron Microscopy" by J. C. H.
Spence, Clarendon Press, Oxford Univ, 1981, ISBN 0-19-851365-8 (I believe
there is a second edition, too)





From: luciom-at-NEWTON.UMSL.EDU (Luciano Mule'Stagno)
Date: Thu, 12 Dec 1996 09:12:27 -0600
Subject: (possible) VIRUS ALERT

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Hi All,
I could not verify if this was a real occurence or propogated hearsay,
but then, better safe than sorry.

cheers

Lucio
} }
} } Subject: Virus Alert
} } Importance: High
} }
} } If anyone receives mail entitled: PENPAL GREETINGS! please delete it
} } WITHOUT reading it. Below is a little explanation of the message, and }
} what it would do to your PC if you were to read the message. If you have
} } any questions or concerns please contact SAF-IA Info Office on 697-5059.
} }
} } This is a warning for all internet users - there is a dangerous virus }
} propogating across the internet through an e-mail message entitled
} } "PENPAL GREETINGS!". DO NOT DOWNLOAD ANY MESSAGE ENTITLED "PENPAL }
} GREETINGS!"
} }
} } This message appears to be a friendly letter asking you if you are
} } interested in a penpal, but by the time you read this letter, it is too }
} late. The "trojan horse" virus will have already infected the boot
} } sector of your hard drive, destroying all of the data present. It is a }
} self-replicating virus, and once the message is read, it will
} } AUTOMATICALLY forward itself to anyone who's e-mail address is
} } present in YOUR mailbox! This virus will DESTROY your hard drive, and
} } holds the potential to DESTROY the hard drive of anyone whose mail is in
} } your inbox, and who's mail is in their inbox, and so on. If this virus }
} remains unchecked, it has the potential to do a great deal of DAMAGE to }
} computer networks worldwide!!!!
} }
} } Please, delete the message entitled "PENPAL GREETINGS!" as soon as you }
} see it! And pass this message along to all of your friends and
} } relatives, and the other readers of the newsgroups and mailing lists
} } which you are on, so that they are not hurt by this dangerous virus!!!! } }
} } ------ =_0_MIME_Boundary_5246.32a5c1cf.imqyy6m0.dcuh029.dcu.ps.net-- } }


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Dr.Lucio Mule'Stagno
MEMC Electronic Materials Inc University of Missouri -St.Louis
Silicon Materials Research Group Physics Dept.,
501 Pearl Dr., 8001 Natural Bridge rd.,
St.Peters, St.Louis
MO 63376 MO 63121
tel: 314 279 5338 314 516 5931/3
fax: 314 279 5363 314 516 6152
email: lmulestagno-at-memc.com luciom-at-newton.umsl.edu
URL:http://newton.umsl.edu/~luciom
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~





From: Mike Witcomb :      MIKEW-at-gecko.biol.wits.ac.za
Date: Thu, 12 Dec 1996 17:29:43 GMT+2
Subject: TEM powder prep - THANKS

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To everyone that offered suggestions of how to proceed with the
preparation of powders for TEM, thanks. We will be trying in
January. Now to get rid of this nasty sunburn from Durban beaches!
You missed a great MSSA conference last week!
To everyone, all good wishes for the festive season.
Mike


Michael J Witcomb PhD
Electron Microscope Unit
University of the Witwatersrand
Private Bag 3
WITS
2050
South Africa

Telephone: + 27 11 716 4000
+ 27 11 716 2419 (messages)
Fax: + 27 11 339 3407
E-mail: mikew-at-gecko.biol.wits.ac.za





From: Tobias Baskin :      baskin-at-biosci.mbp.missouri.edu
Date: Thu, 12 Dec 1996 09:19:01 -0600
Subject: Re: Technovit resin/plant material

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Greetings,
Estela Rossetto asked about freeze substitution protocols for
plant tissue.
The "best" medium will depend on your application, ultrastructure or
immunocytochemistry (or, gasp, both). We have had very nice results with
arabidopsis roots freeze substituted in acetone with 1% dimethoxypropane,
for 48h at about minus 80 C. The dimethoxypropane acts as a chemical water
scavenger so no messy molecular sieve required. Suprisingly, there is no
need to include a fixative with the acetone. We find acceptable results
with ethanol too. We are doing immuno work at the light microscope level,
so we have not analysed this with the rigor of electron microscopy.

And on the resin question, I might add that we enjoy embedding
plant material in butylmethyl methacryalte, which is easy to use and is
removable after sectioning, so is great for immunocytochem (again, at the
light level).

Hope this helps,
Tobias Baskin

_ ____ ^ __ ____ Tobias I. Baskin
/ \ / / \ / \ \ University ofMissouri
/ | / / \ \ \ BiologicalSciences
/___/ /__ /___ \ \ \__ 109 Tucker Hall
/ / / \ \ \ Columbia, MO 65211 USA
/ / / \ \ \ voice: 573-882-0173
/ /____ / \ \__/ \____ fax: 573-882-0123






From: akracher-at-iastate.edu (Alfred Kracher)
Date: Thu, 12 Dec 1996 09:48:10 -0600
Subject: Re: On a quest for a microprobe standard

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} I need a carbonate with low quantities
} (e.g., 0.5-2% by weight) of SrO. Preferably the standard would be homo-
} geneous and the composition known accurately.
} ...
} p.s. the application for which this standard is desired is to measure trace
} quantities of Sr in fish ear-stones.

I have done Sr in carbonates, but have always used a celestite standard.
Sorry I can't help you with a carbonate, but please let me know (off-list)
if you find one. I assume you would like to verify how much Sr you can see?

Good luck,
Alfred

-----------------------------------------
Alfred Kracher
akracher-at-iastate.edu
http://www.public.iastate.edu/~akracher
-----------------------------------------






From: Mark Wall :      Mark.Wall-at-quickmail.llnl.gov
Date: 12 Dec 1996 08:00:35 -0800
Subject: TEM SEG

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I AM STILL LOOKING FOR A SEG (SIDE ENTRY GONIOMETER STAGE, NOT A SPECIMEN
HOLDER) FOR A JEOL TEM. ANY 100 OR 200 MODEL. WE ARE WILLING TO PURCHASE AT A
RESEANONABLE PRICE IF NECESSARY. IF NECESSARY WE COULD PURCHASE THE ENTIRE
TEM.

THANKS

MARK A. WALL
LAWRENCE LIVERMORE NATIONAL LAB
L-350
CHEMISTRY & MATERIALS SCIENCE DEPT.
7000 EAST AVE
LIVERMORE, CA 94550





From: Keith Ryan :      KPR-at-wpo.nerc.ac.uk
Date: Thu, 12 Dec 1996 15:00:56 +0000
Subject: High resolution printer -Reply

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Dear Jacky

I would be interested in a summary/collection.

With best wishes - Keith Ryan

++++++++++++++++++++++++++++++++++++++++++++++++++
Keith Ryan B.Sc., Ph.D., C.Biol., M.I.Biol.
Plymouth Marine Laboratory, Citadel Hill,
Plymouth, Devon PL1 2PB, England

Tel: ++44 1752 633294 (international)
01752 633294 (national)
Fax: ++44 1752 633102 (international)
01752 633102 (national)
e-mail: k.ryan-at-pml.ac.uk
PML web site: http://www.npm.ac.uk/pml
++++++++++++++++++++++++++++++++++++++++++++++++++




From: Caroline Schooley :      schooley-at-mcn.org
Date: Thu, 12 Dec 1996 10:04:21 -0700
Subject: Re: Acrylate Allergies!

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} i do not recall her name off hand, but if you contact rmc,inc
} tucson,arizona,(they probably have a web page) and ask who was their
} biological instructor at their ultra microtomy conference this year in
} oct . I specifically remember, and i was surprised that she repeatedly
} cautioned the group about similar severe allergy-proned
} individual-reactions being rather common. I believe she also may be
} linked to this newsgroup and may reply directly.

I'm that instructor. I don't have any specific medical information; just
the sort of word-of-mouth stories that are appearing here (which I'm saving
for next year's Materials Microtomy course!). I'd appreciate comments from
knowledgable MDs. I know that I read a paper some years ago about similar
reactions to the acrylic resins used to cement metal joints into bone in
orthopaedic surgery...




Caroline Schooley
Educational Outreach Coordinator
Microscopy Society of America
Box 117
Caspar, CA 95420

Phone/FAX (707)964-9460
schooley-at-mcn.org
http://www.MSA.microscopy.com/ProjectMicro/Books.html






From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Thu, 12 Dec 1996 09:05:27 -0800
Subject: Re: High resolution printer

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please send me a summary of the lazarprint results
thanks in advance
steve

---------------------------------------------------------------------

Dr. Steven Barlow
EM Facility/Biology Department
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619)594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
website: http://www.sci.sdsu.edu/EM_Facility






From: akracher-at-iastate.edu (Alfred Kracher)
Date: Thu, 12 Dec 1996 11:14:48 -0600
Subject: Fake VIRUS ALERTs

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The following is directly from the CIAC (U.S. Department of Energy's
Computer Incident Advisory Capability) web page, most recently updated
December 6:

-----

The PENPAL GREETINGS! Hoax shown below appears to be an attempt to kill an
e-mail chain letter by claiming that it is
a self starting Trojan that destroys your hard drive and then sends copies
of itself to everyone whose address in in your
mailbox. Reading an e-mail message does not run it nor does it run any
attachments, so this Trojan must be self starting.
Aside from the fact that a program cannot start itself, the Trojan would
also have to know about every different kind of
e-mail program to be able to forward copies of itself to other people. This
warning is totally a hoax.

-----

I would propose that virus alerts should **not** be posted unless they are
first checked with CIAC or a similar "in the know" location. What do other
subscribers think about this?

The probability that any of us become aware of a virus before an agency
like CIAC does is, I believe, vanishingly small. Fake alerts are clogging
the net at an alarming rate these days, creating a "cry wolf" problem that
actually makes real viruses more dangerous.

Alfred

-----------------------------------------
Alfred Kracher
akracher-at-iastate.edu
http://www.public.iastate.edu/~akracher
-----------------------------------------






From: LARNOULD J. :      larnould-at-mnet.fr
Date: Thu, 12 Dec 1996 18:15:03 +0100
Subject: Re: Regional newspapers

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At 07:55 12/12/1996 G+3, you wrote:
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From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 12 Dec 1996 12:08:10 -0500 (EST)
Subject: Re: Regional newspapers

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} Yeah, that was the silliest thing I've ever seen on this listserver. But
} what is SPAM? I mean besides the canned stuff.

Dear Frank,
A "spam" is a widespread, unsolicited email or newsgroup posting.
Usually these are advertisements, often the return address of the spammer
has been concealed, so that angry replies just clog some poor innocent's
mailbox.
Yours,
Bill Tivol




From: ols0-at-lehigh.edu (Olga L. Shaffer)
Date: Thu, 12 Dec 1996 13:00:52 EST
Subject: Re: Acrylate Allergies!

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Hi:
I myself have through the years developed a reaction to acrylates. My
field is polymers and I am frequently in contact with acrylates. The
monomer is the problem not the polymer. Wear gloves and always work in
the hood. If you do get exposed wash or shower as soon as possible. The
reaction is cumulative and becomes worse with each exposure.
Good luck






From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Thu, 12 Dec 1996 13:04:36 -0500
Subject: Optical Scopes

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I'm thinking of buying a new low power ( {50x) zoom OM for general
inspection work prior to SEM work. One of the other labs here has a
WILD M10. It's a great scope, but I wanted to look around some more.
I've looked in many of the catalogs, etc. but I wanted to get user input
as well.

If you were going to buy a scope today, what would you get? What would
you avoid? What accessories would you get (i.e. ring lights, diffuse
lighting, polarzing filters, etc.)?

I'd like only user input, no sales pitches.


Thanks
-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.siemens.com
--

"Crossman, Harold" {crossman-at-RD.SYLVANIA.com}





From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Thu, 12 Dec 1996 13:04:36 -0500
Subject: Optical Scopes

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I'm thinking of buying a new low power ( {50x) zoom OM for general
inspection work prior to SEM work. One of the other labs here has a
WILD M10. It's a great scope, but I wanted to look around some more.
I've looked in many of the catalogs, etc. but I wanted to get user input
as well.

If you were going to buy a scope today, what would you get? What would
you avoid? What accessories would you get (i.e. ring lights, diffuse
lighting, polarzing filters, etc.)?

I'd like only user input, no sales pitches.


Thanks
-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.siemens.com
--

"Crossman, Harold" {crossman-at-RD.SYLVANIA.com}





From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Thu, 12 Dec 1996 13:04:36 -0500
Subject: Optical Scopes

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I'm thinking of buying a new low power ( {50x) zoom OM for general
inspection work prior to SEM work. One of the other labs here has a
WILD M10. It's a great scope, but I wanted to look around some more.
I've looked in many of the catalogs, etc. but I wanted to get user input
as well.

If you were going to buy a scope today, what would you get? What would
you avoid? What accessories would you get (i.e. ring lights, diffuse
lighting, polarzing filters, etc.)?

I'd like only user input, no sales pitches.


Thanks
-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.siemens.com
--

"Crossman, Harold" {crossman-at-RD.SYLVANIA.com}





From: psic-at-uclink4.berkeley.edu (Paula Sicurello)
Date: Thu, 12 Dec 1996 12:20:58 -0800 (PST)
Subject: Thin sectioning bone

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Hello All!

Has anyone out there thin sectioned undecalcified bone embedded into
epon/araldite? Please relay any information y'all might have, pointers,
etc. on the best way to trim and section this stuff. Can we use a glass
knife? Or does it only cut with diamond?

Thanks, in advance, for all your help.

Paula Sicurello
U.C. Berkeley
Electron Microscope Lab

e-mail: psic-at-uclink4.berkeley.edu






From: Damian_Neuberger_at_RLT013-at-ccmailgw.mcgawpark.baxter.com
Date: Thu, 12 Dec 1996 13:53:58 -0600
Subject: Unwanted messages

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Hello everyone

I am sure that you all have received from time to time messages that
were inappropriate to the listserve, e.g. the recent one about
newspapers. As I can best recall, the response to this and other
message by Nestor, our friendly sysop, was and has been swift and
clear. May I suggest that by letting him handle it we can all save
time, resources, bandwidth? (whatever that is :-)), etc. by not
individually responding, usually with the original message included.

Seasons peace and joy to you all (that's PC for merry Christmas and
happy New Year).

Damian




From: rooney-at-hii.hitachi.com
Date: Thu, 12 Dec 96 15:30:53 PST
Subject: Receipt of 12/12/96 11:48 AM message

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Re:Optical Scopes





From: Marshall (Chip) Montrose :      mmontros-at-welchlink.welch.jhu.edu
Date: Thu, 12 Dec 1996 16:39:25 -0500 (EST)
Subject: JOB OPENING

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TECHNICIAN POSITION
JOHNS HOPKINS UNIV. IMAGING FACILITY

We have an opening for a Research Technician to operate and maintain
imaging and fluorescence analysis equipment which includes a Zeiss
LSM410 confocal microscope, low-light digital imaging microscope,
fluorometers, and image analysis workstations. We are seeking an
individual with strong computer skills (Windows programming experience
highly desirable) as well as experience with advanced light microscopy
and/or lasers and optics. Programming expertise is needed for designing
and implementing new routines for both image acquisition and image
analysis. The successful candidate will be responsible for training and
assisting users from throughout Johns Hopkins University in studies which
involve fixed or living samples, as well as performance of most
administrative aspects of running the facility. The most challenging
portion of the facility is the confocal equipment, so candidates with
experience in confocal microscopy are highly encouraged to apply.

The position is full-time and is available immediately. Salary will be based
on experience and education level of the successful applicant.
Applications will be accepted until the position is filled.

Please send CV, salary requirements, and names of three references to Dr.
Chip Montrose, Ross 930, Johns Hopkins University School of Medicine,
720 Rutland Avenue, Baltimore, MD 21205. Alternatively, e-mail
mhm3-at-jhu.edu or FAX 410- 955-9677.


*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*
Chip Montrose
Johns Hopkins University tel: 410-955-9681
Ross 930 FAX: (410) 955-9677
720 Rutland Avenue email: mmontros-at-welchlink.welch.jhu.edu
Baltimore, MD 21205
*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*-*





From: Doug Keene :      DRK-at-shcc.org
Date: Fri, 13 Dec 1996 20:32:06 -0600 (cst)
Subject: Re: collagen - TEM

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With regard to staining collagen gels, try adding 0.1% w/v
tannic acid to the primary fix (aldehyde). This will
improve the staining of the collagen remarkably.


----------------------
Doug Keene
DRK-at-shcc.org






From: Doug Keene :      DRK-at-shcc.org
Date: Fri, 13 Dec 1996 20:32:06 -0600 (cst)
Subject: Re: collagen - TEM

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With regard to staining collagen gels, try adding 0.1% w/v
tannic acid to the primary fix (aldehyde). This will
improve the staining of the collagen remarkably.


----------------------
Doug Keene
DRK-at-shcc.org






From: DAVID VOWLES :      VOWLES-at-rsbs-central.anu.edu.au
Date: Fri, 13 Dec 1996 09:53:52 EST10
Subject: PCVISIONplus Framegrabber

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I would like to obtain a PCVISIONplus framegrabber (PAL version).
These boards were very popular some years ago for monochrome
image grabbing.

If you have one of these which you are not using and would be willing
to part with it, would you please email me.

David Vowles
Electron Microscopy Unit
Australian National University
PO Box 475
Canberra ACT 2601 Australia
Tel:(616) 2493543 Fax:(616) 2494891
Email: David.Vowles-at-anu.edu.au




From: sbarlow-at-sunstroke.sdsu.edu (Steve Barlow)
Date: Thu, 12 Dec 1996 15:22:31 -0800
Subject: TEM:used Siemens donation

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} } Please forward this message to your scientific departments.
} }
} } The University of Arkansas has a Siemens Electron Microscope in good
} } working order that we would like to surplus. The equipment could be
} } obtained for little more than the cost of transporting & installation.
} }
} } Please contact me if there is any interest in this piece of
} } equipment. Thank you.
} }
} } Jane T. Eaves, CPPO
} } Business Manager
} } 321 Administration Bldg.
} } University of Arkansas
} } Fayetteville, AR 72701
} }
} } Phone 501-575-2551
} } Fax 501-575-4158
} }
} }
}

---------------------------------------------------------------------

Dr. Steven Barlow
EM Facility/Biology Department
5500 Campanile Drive
San Diego CA 92182-4614
phone: (619)594-4523
fax: (619) 594-5676
email: sbarlow-at-sunstroke.sdsu.edu
website: http://www.sci.sdsu.edu/EM_Facility






From: Damian_Neuberger_at_RLT013-at-ccmailgw.mcgawpark.baxter.com
Date: Thu, 12 Dec 1996 13:53:58 -0600
Subject: Unwanted messages

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Hello everyone

I am sure that you all have received from time to time messages that
were inappropriate to the listserve, e.g. the recent one about
newspapers. As I can best recall, the response to this and other
message by Nestor, our friendly sysop, was and has been swift and
clear. May I suggest that by letting him handle it we can all save
time, resources, bandwidth? (whatever that is :-)), etc. by not
individually responding, usually with the original message included.

Seasons peace and joy to you all (that's PC for merry Christmas and
happy New Year).

Damian




From: Vladimir Dusevich :      dusevich-at-astro.ocis.temple.edu
Date: Thu, 12 Dec 1996 18:34:57 -0500 (EST)
Subject: Re: Fake VIRUS ALERTs

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YES!!!

On Thu, 12 Dec 1996, Alfred Kracher wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} The following is directly from the CIAC (U.S. Department of Energy's
} Computer Incident Advisory Capability) web page, most recently updated
} December 6:
}
} -----
}
} The PENPAL GREETINGS! Hoax shown below appears to be an attempt to kill an
} e-mail chain letter by claiming that it is
} a self starting Trojan that destroys your hard drive and then sends copies
} of itself to everyone whose address in in your
} mailbox. Reading an e-mail message does not run it nor does it run any
} attachments, so this Trojan must be self starting.
} Aside from the fact that a program cannot start itself, the Trojan would
} also have to know about every different kind of
} e-mail program to be able to forward copies of itself to other people. This
} warning is totally a hoax.
}
} -----
}
} I would propose that virus alerts should **not** be posted unless they are
} first checked with CIAC or a similar "in the know" location. What do other
} subscribers think about this?
}
} The probability that any of us become aware of a virus before an agency
} like CIAC does is, I believe, vanishingly small. Fake alerts are clogging
} the net at an alarming rate these days, creating a "cry wolf" problem that
} actually makes real viruses more dangerous.
}
} Alfred
}
} -----------------------------------------
} Alfred Kracher
} akracher-at-iastate.edu
} http://www.public.iastate.edu/~akracher
} -----------------------------------------
}
}




From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Thu, 12 Dec 1996 13:04:36 -0500
Subject: Optical Scopes

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I'm thinking of buying a new low power ( {50x) zoom OM for general
inspection work prior to SEM work. One of the other labs here has a
WILD M10. It's a great scope, but I wanted to look around some more.
I've looked in many of the catalogs, etc. but I wanted to get user input
as well.

If you were going to buy a scope today, what would you get? What would
you avoid? What accessories would you get (i.e. ring lights, diffuse
lighting, polarzing filters, etc.)?

I'd like only user input, no sales pitches.


Thanks
-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.siemens.com
--

"Crossman, Harold" {crossman-at-RD.SYLVANIA.com}





From: darrell_miles-at-vnet.ibm.com
Date: Thu, 12 Dec 96 18:21:11 EST
Subject: Re: Acrylate Allergies!

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Hi,

Here at IBM, there is a fairly extensive Industrial Safety and Hygiene
program. In our training classes, it was explained to us that epoxies,
etc., are "sensitizers" as are Poison Ivy, Poison Oak, and Poison Sumac.
Some people may have a reaction on their first exposure. Others may
have a reaction after repeated exposures have "sensitized" them. The
reactions will most likely increase in sevearity with each additional
exposure. There are a few that never show a reaction. I know people
that were never affected by Poison Ivy when they were children, but
had severe reactions when exposed as an adult.

Precautions during use (what we are required to do):
* The resins and hardeners are stored in a vented cabinet.
* Weighing, mixing, and sitting to cure are done in a vented hood.
*** Protective Appearal ***
*** Nitrile Gloves - eg. Nitrilite by Ansell Edmont
Note: Latex gloves were not accepted
* Goggles
* Plastic Chemical Apron

Hope this helps.

Darrell Miles
darrell_miles-at-vnet.ibm.com

IBM Analytical and Test Services
http://www.chips.ibm.com/services/asg/




From: geoffa-at-amsg.austmus.gov.au (GeoffA)
Date: Fri, 13 Dec 1996 11:36:57 +1000
Subject: Re: Optical Scopes

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} I'm thinking of buying a new low power ( {50x) zoom OM ...
} ...I wanted to get user input as well.
} -------------------------------------------------
} Harold J. Crossman
} OSRAM SYLVANIA INC.

____________________ Reply Separator_________________________

Harold,

I've used a Zeiss SV8 for about 10 years and have no reason to even
think of updating it. I chose it over the (then) Wild for it's large
zoom range and interchangeable objectives (the old Wild's had horrible
'add-on' 2x objectives). It might be worth finding out whether that
M10 in the other lab takes interchangeable or supplementary
objectives.

Obviously, the choice of scope and accessories will depend on your
work and budget. I lay out sub-millimetre mollusc radulae and then go
about spreading the teeth on them. Indispensible accessories are;
* fibre optics (rather than built-in light) to get low angles to make
'invisible' specimens cast a shadow,
* side-action micrometre stage (for transferring REALLY small specs; I
can grab a spec, lift it up while racking up the focus, use the stage
to bring over the next container or stub, lower it while racking down,
i.e. keeping my eyes firmly glued on the little sucker through the
whole process),
* 2x objective and 16x eyepieces (total mag of 205x - yes, rather
fuzzy :-)

I also have a neat monocular phototube for it which slides from one
light path to the other allowing sequential stereopairs.

I'm of the view that quality lasts and would automatically be drawn to
Leica and Zeiss. Either of their top shelf models would be good;
certainly the Zeiss SV11 that I've tried is a superb instrument. I
have no financial interest in either but, as implied earlier, I've
never regretted finding the extra dollars for my Zeiss.

Geoff Avern
Microscopy Labs
Australian Museum





From: normand laurier :      laurier-at-nationalnet.com
Date: Thu, 12 Dec 1996 20:55:39 -0500
Subject: Re: Optical Scopes

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Hi
I am related to sales but wont give you a sales pitch just advice. M10 is an
APO type lens which mean that you won`t see those usually yellow fringes
around the specimens but also probably the most expensive microscope. If you
can afford it, it is the best.
Now it depends of you and your applications, The first criteria would be to
ask yourself what you exactly plan to do with it now and latter.
1)is resolution important?
2)do you observe,I think so, flat specimens if yes a Plan objective would be
preferable.(it wont give you this dome or cavity effect when you observe
flat specimen)
3)do you also need transmitted light observation like bright field and/or
may be darkfield illumination.Polarisation is sometimes usefull to get rid
of glare but it as to be tried.
4)Reflected light Illumation is very important. I would trend to look for
fiber optic more than neon especialy for the brightness and versatility.
You should try for ring light as well as flexible twin arm.If you plan to
take color pictures neon light is not very good.
5)Will you spent a lot of time observing, if yes, a variable angle tube
would be very usefull.
6)What is the lowest magnification that you feel comfortable to work with.
Hig quality stereo are incline to aim for hihg magnifications not low. ,This
is where you see realy a difference in resolution. at low power it is much
more difficult to see it. Check if you are using a zomm if the brightness
remain constant or acceptable when you zoom from low to high magnification.
It is not very convenient if you have to adjust the light intensity when you
zoom from low power to high.
Finally why don`t you get in touch with some salesmen and ask for demo and
purchase from the one you trust the most,give you the best advise and
doesn`t give you any bullsheet and try to sell what meet your expectations
and needs.
Norm
At 13:04 12/12/96 -0500, you wrote:
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..................
normand laurier
laurier-at-nationalnet.com
http://www.nationalnet.com/~laurier






From: manuela :      manuelap-at-petermac.unimelb.edu.au
Date: Fri, 13 Dec 1996 12:03:02 +1100
Subject: Re: High resolution printer

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At 09:10 12/12/96 +0100, you wrote:
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Manuela





From: rutledge phil :      prutle1-at-gl.umbc.edu
Date: Thu, 12 Dec 1996 14:30:29 -0500 (EST)
Subject: EM Services

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X-Authentication-Warning: umbc8.umbc.edu: prutle1 owned process doing -bs

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This is to serve notice,by order of the Chair, Dr. Lasse Lindahl, the
Electron Microscopy Facility located at The University of Maryland
Baltimore County,Biology Dept. will no longer be accepting contractual
electron miocroscopy requests.
I would like to thank all of the private companies, universities
and federal and state agencies who helped to make this lab a success.

MERRY CHRISTMAS TO ALL AND TO ALL A GOOD NIGHT! HO-HO-HO



Peace through Christ,

Phil Rutledge, Director e-mail: prutle1-at-gl.umbc.edu
Center for Microscopy voice: (410) 455-3582
UMBC Dept. of Biology fax: (410) 455-3875
Catonsville, MD 21228
/////
/ /
/ /
/////// ///////
/ /
/////// ///////
/ /
/ /
/ /
/ /
/ /
/ /
/////





From: Warren Straszheim :      wes-at-ameslab.gov
Date: Thu, 12 Dec 1996 12:18:38 -0600
Subject: Re: (possible) VIRUS ALERT

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Looks pretty bogus to me, and a lot like the Good Times virus hoax. You
still don't get viruses from reading e-mail. The closest you come is
executing attachments without checking them for viruses.

My Eudora allows me to double-click on the attachment name and bring up the
file. If it was a DOC file, then Word would be opened. If it was an
executable, then it would run. That would not be good as I would risk
infecting my computer. Even opening a Word file that way bypasses the
protection that MS provided against macro viruses. I recall that that
protection only worked if a document was accessed from File/Open.

At 09:12 AM 12/12/96 -0600, you wrote:
} Hi All,
} I could not verify if this was a real occurence or propogated hearsay,
} but then, better safe than sorry.
}
} } } Subject: Virus Alert
} } } Importance: High
} } } If anyone receives mail entitled: PENPAL GREETINGS! please delete it
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Doug Keene :      DRK-at-shcc.org
Date: Sat, 14 Dec 1996 01:31:49 -0600 (cst)
Subject: Re: Thin sectioning bone

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With regard to cutting bone, we fix as usual (1.5/1.5 w/
tannic acid; 1%OsO4; dehydrate and embed in Spurrs epoxy.
We do not decalcify. Bone cuts with difficulty and will
quickly trash a glass knife. Though bone will certainly
decrease the life expectancy of a diamond, it seems the
method of choice. A knife of a wider angle might help with
the problem of edge deterioration, but we use standard
knives in this lab.

Good luck,
----------------------
Doug Keene
DRK-at-shcc.org






From: Doug Keene :      DRK-at-shcc.org
Date: Sat, 14 Dec 1996 01:31:49 -0600 (cst)
Subject: Re: Thin sectioning bone

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With regard to cutting bone, we fix as usual (1.5/1.5 w/
tannic acid; 1%OsO4; dehydrate and embed in Spurrs epoxy.
We do not decalcify. Bone cuts with difficulty and will
quickly trash a glass knife. Though bone will certainly
decrease the life expectancy of a diamond, it seems the
method of choice. A knife of a wider angle might help with
the problem of edge deterioration, but we use standard
knives in this lab.

Good luck,
----------------------
Doug Keene
DRK-at-shcc.org






From: normand laurier :      laurier-at-nationalnet.com
Date: Fri, 13 Dec 1996 00:15:39 -0500
Subject: Re: Thin sectioning bone

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Hi
Depend of the size of the specimen. I have a customer who use a saw
microtome from Leitz to obtain bones sections of about 50 microns thick.
(pigs bones)
If you need her coordonates please let me know. Sections were used for
photonic observations not EM.
Norm
At 12:20 12/12/96 -0800, you wrote:
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..................
normand laurier
laurier-at-nationalnet.com
http://www.nationalnet.com/~laurier






From: Jouko =?iso-8859-1?Q?M=E4ki?= :      jouko.maki-at-utu.fi
Date: Fri, 13 Dec 1996 07:55:54 +0200
Subject: Re: TEM SEG

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X-Sender: jokamaki-at-mailhost.utu.fi
X-Mailer: Windows Eudora Light Version 1.5.2
Mime-Version: 1.0
Content-Type: text/plain; charset="iso-8859-1"
Content-Transfer-Encoding: quoted-printable
To: Microscopy-at-Sparc5.Microscopy.Com

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At 08:00 12.12.1996 -0800, you wrote:
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America=20
A
} RESEANONABLE PRICE IF NECESSARY. IF NECESSARY WE COULD PURCHASE THE ENTIRE
} TEM.

Mark,

My message from Nov. 28th is still valid.

Jouko

Jouko K. M=E4ki, Ph.D., Laboratory Manager
University of Turku, Laboratory of Electron Microscopy
Kiinamyllynkatu 10 FIN-20520 TURKU FINLAND
Tel: + 358 2 333 7318 GSM: + 358 40 505 2521 FAX: + 358 2 333 7380





From: Charlotte Clausen Appel, 5770. :      c.c.appel-at-risoe.dk
Date: Fri, 13 Dec 1996 08:29:08 +0100
Subject: RCPT: TEM powder prep - THANKS

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Confirmation of reading: your message -

Date: 12 Dec 96 17:29
To: microscopy-at-Sparc5.Microscopy.Com
Subject: TEM powder prep - THANKS

Was read at 8:29, 13 Dec 96.





From: Joergen Bilde-Soerensen 5802 :      j.bilde-at-risoe.dk
Date: Fri, 13 Dec 1996 08:38:59 +0100
Subject: RCPT: TEM powder prep - THANKS

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Confirmation of reading: your message -

Date: 12 Dec 96 17:29
To: microscopy-at-Sparc5.Microscopy.Com
Subject: TEM powder prep - THANKS

Was read at 8:38, 13 Dec 96.

-at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at--at-
J. B. Bilde-Soerensen
Materials Department
Risoe National Laboratory
DK-4000 Roskilde
Denmark

e-mail: j.bilde-at-risoe.dk
phone: +45 46 77 58 02 (direct)
phone: +45 46 77 46 77 (switchboard)
fax: +45 46 35 11 73




From: R. BEKENKAMP :      R.Bekenkamp-at-lct.fnt.hvu.nl
Date: Fri, 13 Dec 1996 08:41:58 +0000 (GMT)
Subject: RCPT: TEM powder prep - THANKS

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Bevestiging van lezing : uw bericht -

Datum: 12 Dec 96 17:29
Aan: microscopy-at-Sparc5.Microscopy.Com
Ondw.: TEM powder prep - THANKS

Gelezen om 8:41, 13 Dec 96.


-----
| o | R.Bekenkamp
| I | Hogeschool van Utrecht
| I | Faculteit Natuur en Techniek
| I | Afd. LCT
--H-- Holland
I Tel 030-2712404
I Fax 030-2730905
I E-mail R.Bekenkamp-at-lct.fnt.hvu.nl
{ }




From: MAILER-DAEMON-at-VINES.UCI.KUN.NL
Date: Fri, 13 Dec 1996 09:26:01 +0000
Subject: Optical Scopes

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To: "'microscopy'" {Microscopy-at-Sparc5.Microscopy.COM}
Cc:

The above message has been received by:

H. Smits-at-celbi-at-fmw




From: MAILER-DAEMON-at-VINES.UCI.KUN.NL
Date: Fri, 13 Dec 1996 09:26:31 +0000
Subject: Unwanted messages

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To: {microscopy-at-Sparc5.Microscopy.COM}
Cc:

The above message has been received by:

H. Smits-at-celbi-at-fmw




From: MAILER-DAEMON-at-VINES.UCI.KUN.NL
Date: Fri, 13 Dec 1996 09:40:38 +0000
Subject: Optical Scopes

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To: "'microscopy'" {Microscopy-at-Sparc5.Microscopy.COM}
Cc:

The above message has been received by:

H. Smits-at-celbi-at-fmw




From: micrick-at-earthlink.net (Rick Ellis)
Date: Fri, 13 Dec 1996 01:22:39 -0800 (PST)
Subject: Optical Scopes

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Please disregard for the present our notification of new e-mail address.
(We had hoped to be micrick5-at-home.com by now, but have had an installation
problem.) We are still getting messages via Earthlink address, altho we
were supposed to have cancelled it as of Dec 10.






From: wahlbrin-at-crpcu.lu (Petra Wahlbring)
Date: Fri, 13 Dec 96 10:31 MET
Subject: Re: TEM phosphor plates vs. CCD camera systems

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} What are the advantages/disadvantages (including price) in using the
} reusable TEM phosphor plates vs. a dedicated CCD camera system for
} collecting images?
}
} Thanks.

} Lucille A. Giannuzzi, Ph.D.

I am not an expert on this field, but I worked once with this kind of
imaging plate system in Japan.

One very obvious difference to an CCD-array is the size of the imaging
plate. It has the same format like an good old photo film. The size of
a CCD-array is a few square-cm in the best case. Therefor you have to
reduce the magnification if you want to record the image you saw on your
final screen.

On the other hand, with a CCD you have direct access to the image data.
An imaging plate must be processed in its reader first, a process that
can take several minutes at best.

For all technical details (sensitivity, dynamic range, ...) I would
recommend you to read

Mori, N et al.: Application of the "imaging plate" to TEM image recording.
Ultramicroscopy 25 (1988) 195-202

or

Mori, N et al.: Development of the imaging plate for the transmission
electron microscope and its characteristics. J. Electron Microsc. 39 (1990)
433-436

Petra Wahlbring

--------------------------------------------------------------
Dr. Petra Wahlbring
Centre de Recherche Public Centre Universitaire (CRP-CU)
Laboratoire d'Analyse des Mat=E9riaux (LAM)
162a, av. de la Fa=EFencerie L-1511 Luxembourg
tel. +352-466644-402 fax +352-466644-400
e-mail: petra.wahlbring-at-crpcu.lu or 100112.2335-at-compuserve.com





From: Jan Kozubowski :      JANKOZU-at-inmat.pw.edu.pl
Date: Fri, 13 Dec 1996 10:58:16 CET
Subject: RCPT: TEM powder prep - THANKS

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Confirmation of reading: your message -

Date: 12 Dec 96 17:29
To: microscopy-at-Sparc5.Microscopy.Com
Subject: TEM powder prep - THANKS

Was read at 10:58, 13 Dec 96.





From: Mnr HJ Els :      HJELS-at-op1.up.ac.za
Date: Fri, 13 Dec 1996 12:34:55 GMT+2
Subject: RCPT: TEM powder prep - THANKS

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Confirmation of reading: your message -

Date: 12 Dec 96 17:29
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Subject: TEM powder prep - THANKS

Was read at 12:34, 13 Dec 96.





From: nano-at-ns1.lihti.org (Nanoprobes Inc.)
Date: Fri, 13 Dec 1996 06:58:25 -0500
Subject: Re: Fake VIRUS ALERTs

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The DOE-CIAC web site is at

http://ciac.llnl.gov/

and the hoaxes page

http://ciac.llnl.gov/ciac/CIACHoaxes.html

} The following is directly from the CIAC (U.S. Department of Energy's
} Computer Incident Advisory Capability) web page, most recently updated
} December 6:
}
} -----
}
} The PENPAL GREETINGS! Hoax shown below appears to be an attempt to kill an
} e-mail chain letter by claiming that it is
} a self starting Trojan that destroys your hard drive and then sends copies
} of itself to everyone whose address in in your
} mailbox. Reading an e-mail message does not run it nor does it run any
} attachments, so this Trojan must be self starting.
} Aside from the fact that a program cannot start itself, the Trojan would
} also have to know about every different kind of
} e-mail program to be able to forward copies of itself to other people. This
} warning is totally a hoax.
}
} -----
}
} I would propose that virus alerts should **not** be posted unless they are
} first checked with CIAC or a similar "in the know" location. What do other
} subscribers think about this?
}
} The probability that any of us become aware of a virus before an agency
} like CIAC does is, I believe, vanishingly small. Fake alerts are clogging
} the net at an alarming rate these days, creating a "cry wolf" problem that
} actually makes real viruses more dangerous.
}
} Alfred
}
} -----------------------------------------
} Alfred Kracher
} akracher-at-iastate.edu
} http://www.public.iastate.edu/~akracher
} -----------------------------------------

Rick Powell






From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Fri, 13 Dec 1996 08:42:34 -0400 (AST)
Subject: Re: Regional newspapers

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In message Thu, 12 Dec 1996 12:08:10 -0500 (EST),
William Tivol {tivol-at-wadsworth.org} writes:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
}
} } Yeah, that was the silliest thing I've ever seen on this listserver.
} } But what is SPAM? I mean besides the canned stuff.
} }
}
} Dear Frank,
} A "spam" is a widespread, unsolicited email or newsgroup posting.
} Usually these are advertisements, often the return address of the spammer
} has been concealed, so that angry replies just clog some poor innocent's
} mailbox.
} Yours,
} Bill Tivol
}
Thanks, Bill. There always seems to be some new jargon or slang
expression to catch up on.
F.C. Thomas
GSC (Atlantic) MicroAnalysis Facility
P.O. Box 1006, Dartmouth, N.S.
B2Y 4A2
(902) 426-4635




From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Fri, 13 Dec 1996 07:38:28 -0500
Subject: Adminitrivia

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G'day Subscribers.....

Yes I have seen that, contrary to the rule, that h.smits-at-celbi.kun.nl
has set his/her Email to *automatically reply* to each message. I
have removed him/her from the Microscopy Listserver, until such
time as that is fixed at his/her end.

I've not had any more reports of blank messages, so I am assuming
that the problem is now cured.

For the record, will those of you that had the problem (i.e. receiving
the banner only and then no message. Please send me an Email message
I would like the following information (or as much as you can provide).

Your Email Address
Your Email Service Provider (My Company, AOL, .....)
& Their Computer System (i.e IBM Mainframe, Sun Server .etc....)
& Their Service (Direct Login, POP3, SMTP.....)
Your Email Program (Eudora, Pegasus, White Pine, Netscape......)

I'd like to see what the common characteristics were of the sites that
had the problem as it was NOT universal.

Thanks...






From: John_Martin_at_notes-at-smtp.cspi.com
Date: Fri, 13 Dec 96 07:48:00 EST
Subject: Receipt: Message Received at 12-13-96 07:50:00

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Re: Optical Scopes





From: Jennifer_Kramer_at_notes-at-smtp.cspi.com
Date: Fri, 13 Dec 96 09:06:00 EST
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Re: Optical Scopes





From: efosten-at-MMM.COM
Date: Fri, 13 Dec 1996 09:08:24 -0600
Subject: Re: Optical Scopes

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Harold,

As long as you're going to use an OM at ' {50X for general inspection work
prior to SEM work' then any of the standard OM brands will suffice (we have
a Wild M3Z in our SEM/TEM lab, do most of our pre-EM OM work in the 10-50X
range, and are very satisfied with the Wild). More important (since you
are doing, in effect, correlative microscopy) would be your illumination
options - get lots. We use ring, coaxial, transmitted (bright and dark
fields), and fiberoptic oblique (frequency of use in about that order). I
find coaxial to be especially useful when going from the OM to the SEM.
Also get a recording option (Polaroid, video printing).


At 01:04 PM 12/12/96 -0500, you wrote:
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From: Buddy Steffens :      STEFFENS.B-at-calc.vet.uga.edu
Date: Fri, 13 Dec 1996 10:44:35 EST
Subject: Re: Thin sectioning bone

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Paula,

We have cut undecalcified avian growth plate from the point where it
is hyaline cartilage down to the actual bone. We always used Spurr
resin, although E/A would probably work fine. Our sections were
HUGE, measuring at least 2x2 mm and were mounted on slot grids. We
used a new diamond knife which was dedicated to this project,
assuming that it would get dull and scratchy quickly. After
thousands of sections, it showed no more wear and tear than a diamond
used for more mundane purposes. A glass knife probably would have
sufficed. Unless you are working with a particularly dense bone, you
shouldn't have any unusual problems.



-=W.L. Steffens=-
Department of Veterinary Pathology
College of Veterinary Medicine
University of Georgia




From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: Fri, 13 Dec 1996 07:52:25 -0800
Subject: Re: Optical Scopes

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From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: 12/12/96 1:04 PM
Subject: Optical Scopes

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Harold;

My pre-EM microscope is actually a hybrid, made from parts of scopes we had
around here. I combined a Nikon SMZ-2T (10-63x zoom and photo tube) with an
Olympus stage/base to provide me with reflected, dark and bright field lighting.
I am sure that most manufacturers can provide you with these options in a single
scope; for me money was an issue at the time. The scope is inside of a laminar
flow hood so that I can do relatively contaminant-free prep work. This seems to
serve most of my needs quite well. My personal experience is that these are
probably the minimum accessories you would require. I use a separate research
grade scope (Zeiss) for specialized applications such as the use of polarizing
filters, Nomarski, digital imaging, image analysis, etc.

Best Regards,

-Bob
***********************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA 91711
(909)399-1311
email: Bob_Citron-at-cc.chiron.com
***********************************
______________________________ Reply Separator _________________________________


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I'm thinking of buying a new low power ( {50x) zoom OM for general
inspection work prior to SEM work. One of the other labs here has a WILD
M10. It's a great scope, but I wanted to look around some more. I've
looked in many of the catalogs, etc. but I wanted to get user input as
well.

If you were going to buy a scope today, what would you get? What would
you avoid? What accessories would you get (i.e. ring lights, diffuse
lighting, polarzing filters, etc.)?

I'd like only user input, no sales pitches.


Thanks
-------------------------------------------------
Harold J. Crossman
OSRAM SYLVANIA INC.
Lighting Research Center
71 Cherry Hill Dr.
Beverly, MA 01915
Phone: (508) 750-1717
E-mail: crossman-at-rd.sylvania.com

Our web sites: www.sylvania.com
www.siemens.com
--

"Crossman, Harold" {crossman-at-RD.SYLVANIA.com}





From: Gary Chandler :      gwc-at-platinum.sem.Arizona.EDU
Date: Fri, 13 Dec 1996 09:20:33 -0700 (MST)
Subject: Re: Optical Scopes

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{c=US%a=_%p=SYLVANIA%l=RD_EXC1-961212180436Z-10568-at-da-exc1.sylvania.com}
To: "'microscopy'" {Microscopy-at-Sparc5.Microscopy.Com}
Message-id: {Pine.GSO.3.94.961213085649.27658B-100000-at-platinum.sem.arizona.edu}
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From: Gary Chandler :      gwc-at-platinum.sem.Arizona.EDU
Date: Fri, 13 Dec 1996 09:20:33 -0700 (MST)
Subject: Re: Optical Scopes

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On Thu, 12 Dec 1996, Crossman, Harold wrote:

} I'm thinking of buying a new low power ( {50x) zoom OM for general
} inspection work prior to SEM work. One of the other labs here has a

} you avoid? What accessories would you get (i.e. ring lights, diffuse
} lighting, polarzing filters, etc.)?


Harold,
I use our scope (Nikon) routinely and particularly like the
fiber-optic light source, Lumina-I. This has the choice of a ring for even
illumination of the smaple, or a two-pronged, bendable source to
customize oblique illumination. This was purchased over five years ago, so
I don't know current vendors, prices, etc. We also have ours fitted to a
video camera. This is relatively inexpensive and gives you the options of
thermal printers rather than film, image on a screen for
viewing by a group, video-capture with computer, etc. I
believe most microscopes now have the option of a tripod eyepiece,
useful for adding the video camera.

Gary Chandler
Materials Science and Engineering
University of Arizona





From: lizard-at-okway.okstate.edu (Ginger Baker)
Date: Fri, 13 Dec 1996 10:19:53 -0800
Subject: SEM Filter Replies

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Thank you to all who replied to my query concerning examination of filter=
s =

containing bacteria. Here are a list of the responses I received.
=

Ginger Baker
EM Lab Manager
Dept. of Anatomy, Pathology, and Pharmacology
250 Veterinary Medicine
Oklahoma State University
Stillwater, OK 74078
(405) 744-6765
FAX: (405) 744-5275
Email: lizard-at-okway.okstate.edu
=

=

(1) Some bacteria can stand airdrying. I suggest trying this with part o=
f the =

sample. If the bacteria or other organisms look deflated you can CPD the=
=

remainder.

Dave


(2) I have done similar preps while at UMASS. Are the bacteria already =

on the filters? There are several kinds of filters. Some are better tha=
n =

others for SEM. The best ones to use for SEM are polycarbonate (Nuclepo=
re =

type, Bio-Rad also sold some). Other ones are a torturous path type and =
it will
be hard to find the bacteria on them.

Your best bet if there is a ?? is to look at an unused filter so you know=

what the background is. Polycarbonate ones are smooth with round holes i=
n them.
They come in several pore sizes. You will probably want ~.2um for bacter=
ia.
Too many bacteria will clog them up and stop the flow. My guess is these=
are
some type of water filters. Certainly try to get a control to look at.

By all means fix the samples, osmicate, ethanol dehydrate, CPD and Au/Pd =
coat
for best results. Fixing should also aid in keeping the bacteria in plac=
e.
You can try air drying for comparison but the morphology will
be poor. It depends on what your final purpose is; bacterial counts or
being able to identify types and have nice morphology. I would recommend=
=

running a test batch first to be sure there are no problems. Feel free
to call if you have any ???

Ed Basgall, PhD
Penn State Univ.
Dept. of Chemistry
University Park, PA 16802
Ph: 814-865-0493


(3) If the samples haven't already been filtered, then first sputter
coat the filters before use, or use silver filters. Fewer imaging proble=
ms
that way. With the bacteria on the filters, fix, dehydrate, and dry from
hexamethyldisilizane at room temp or 60 C in a fume hood! I usually get
excellent results this way.
(Note: check Crang and Klomperens "Artefacts in Biological EM", title =

approximate, about fixing and dehydrating and their effects on bacterial =
coats. =

The slime coat may be important, and it's usually lost.)
Phil

&&& Illigitimi non carborundum &&&&&&&&
Philip Oshel
Microscopy
Station A
PO Box 5037
Champaign, IL 61825-5037
(217)244-3145 days
(217)355-3145 evenings
oshel-at-ux1.cso.uiuc.edu

(4) } These kinds of samples really do have to be critical point dried, if=

} } examination is contemplated in a "conventional" SEM. In some instance=
s,
} } using silver membranes makes it possible to get away with looking at t=
he now
} } CPD's bacteria with far less or no metallization, an advantage in some=

} } situations. More information about silver membrane filters can be fou=
nd on
} } our website given below.
} }
} } Chuck
} }
} } =3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=
=3D=3D=3D=3D
} } Charles A. Garber, Ph. D.
} e-mail:cgarber-at-2spi.com
} } PRESIDENT
} } SPI SUPPLIES/STRUCTURE PROBE, INC.
} } WEST CHESTER, PA 19381-0656 USA
} }
} } Look us up at
} } xxxxxxxxxxxxxxxxx
} } http://www.2spi.com
} } xxxxxxxxxxxxxxxxx


(5) I would try to minimize handling as much as possible to avoid losing =
any
bacteria. We have resorted to exposure to osmium vapors for an extended
period such as 24-72 hours and then air drying.

G.W. Erdos, Ph.D. Phone: 352-392-1295 =

Scientific Director, =
=

ICBR Electron Microscopy Core Lab =
=

218 Carr Hall Fax: 352-846-0251 =

University of Florida E-mail: gwe-at-biotech.ufl.edu
Gainesville, FL 32611 http://www.biotech.ufl.edu/~em=
cl/

(6) You presumably have a bacteriology group in your vet school. Ask the=
m
about acridine orange or DAPI as stains for bacteria on memebrane filters=
=2E
Rob Palmer
CEB/UT


(7) If you have a filter to play with, I would first attempt to simply ai=
r dry =

it, perhaps under laminar flow. I've done this before with samples that =
were =

surface-innoculated with bacillus subtillus. Obviously, some of the =

bacteria were collapsed from the vacuum, but the majority of them were in=
tact =

and made quite a nice SEM image when sputter coated with about 10 nm of A=
u:Pd.
Regards,
-Bob
************************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA 91711
(909)399-1311
Bob_Citron-at-cc.chiron.com

(8) I have looked at many bacterial samples,particularly sulfate reducers=
, by =

both air drying and fixation, dehydration, and CPD of filters and often g=
et =

similar results. If there is a capsule or extracellular material of intre=
st the =

fix, dehyd, CPD route may be needed but why not air dry a piece of the fi=
lter =

for a quick look.

(9) Once you filter the suspension of bacteria through a 0.2um polycarbon=
ate =

filter, remove the filter from the housing and place it into fixative (2.=
5% =

glutaraldehyde in a buffer) follow this with:
buffer washes,
an optional post-fixation (osmium tetroxide)
buffer washes
gradient alcohol series (3X100% ETOH)

At this point you have 2 options;
3 washes in HMDS (hexamethyldisilizane)
followed by air-drying
or Critical Point Drying.

Fix the filters to the SEM stub with double-sided
sticky tape, add a drop of silver dag to the edge of
the filter and sputter-coat.

Additional tips---during the intial stage of filtering,
remove the syringe from the filter housing, pull
plunger back, reattach and push a column of air
through the housing---this pushes all of the filtrate
through the filter so that it doesn't drain off when
removing the filter from the Swinney holder.

The filter housing and filter can be autoclaved ahead of time and sterile=
1 cc. =

syringes used to insure a reliable prep.
Best of luck,
Rosemary Walsh

(10) I would definitely recommend to dehydrate and CPD the filters with
the bacteria. After they are dry, you can cut the filter for suitable siz=
e
to be mounted on a SEM-stud, whereafter they have to be coated for conduc=
tivity.
Good luck and happy holidays

Jouko

Jouko K. M=E4ki, Ph.D., Laboratory Manager
University of Turku, Laboratory of Electron Microscopy
Kiinamyllynkatu 10 FIN-20520 TURKU FINLAND
Tel: + 358 2 333 7318 GSM: + 358 40 505 2521 FAX: + 358 2 333 73=
80

(11) Microscopy & Analysis (US Edition Issue No. 21, November 1996) ran a=
n
article you might be interested in - "Cryo-preparation of small or lightl=
y
attached biological specimens" by Robinson et al.

Basically, the idea is a variation on cryo-fixation - this normally
involves plunging a specimen into liquid N2. In the case of specimens
similar to yours, the result is that all the particles of interest drop
off! However, if you simply fix the specimen on to a stub at room
temperature and then transfer it to the pre-cooled cryo-stage, freezing i=
s
still relatively rapid. Obviously, you still loose internal specimen deta=
il
because of ice crystal growth but a considerable amount of external detai=
l
is successfully preserved. The authors present a number of good SEM image=
s.
Whether the procedure will work successfully with specimens as small as
bacteria, I'm not sure.

If you want further details, contact Ken Robinson at KRO-at-pcmail.nerc-bas.=
ac.uk.

Regards,

---------------------------------------------------------------
Dr L. P. Stoter Technical Editor, MICROSCOPY & ANALYSIS
Technesis
17, Rocks Park Road email: LPS-at-teknesis.demon.co.uk
Uckfield, E. Sussex Phone: +44 (0)1825 766911
TN22 2AT Fax: +44 (0)1825 766911
United Kingdom

(12) Use ESEM ( environmental scanning electron microscope) equipped with=
a cold
stage going down to 1-2 degrees C. You will be able to use wet filter wit=
h a =

bacterial deposit on it and to dry carefully water out while in the =

microscope. For a short time ~ 5 minutes, you should be able to see and =

identify your bacteria without substantial distortion. Alternatively, fix=
=

them with 1% of OsO4 in water while on filters and repeat as above. Use 2=
0 =

kV and high condenser setting (60-70%) for artefact free observation.
Cheers,
Wis Jablonski OiC EM/X-ray Microanalysis, CSL, University of Tasmania

(13) I would fix, dehydrate etc. just as you would for any biological spe=
cimen.

Regards,
Marilyn Henderson
CEMMSA
The University of Adelaide
South Australia









From: rw9-at-psu.edu (Rosemary Walsh)
Date: Fri, 13 Dec 1996 11:34:06 -0500
Subject: Re: Optical Scopes

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Harold,
We purchased an Olympus SZ60 with a
45 deg. prism for SZ stereo, 1X-6.3X, 10X
eyepieces + a doubler lens, 2.5 X photoeyepiece,
iris light source, dual flexible gooseneck
fiberoptic illumination with focus heads.
We added an extension pillar to obtain 100mm
working distance.
We've interfaced this with a CCD-TV camera,
monitor and color printer as well as an adaptor
for a 35mm Pentax thread.
We dealt with Dave Williams from HiTech
Instruments (610) 353-3505 and found him
knowledgeable, able to address our needs and
budget constraints.
Rosemary

####################################################
Rosemary Walsh
Electron Microscope Facility for the Life Sciences
The Biotechnology Institute for Research and Education
1 South Frear Lab
University Park, PA 16802
814-865-0212 email:rw9-at-psu.edu
####################################################






From: yuhui xu :      Yuhui=Xu%RES%DFCI-at-EYE.DFCI.HARVARD.EDU
Date: Fri, 13 Dec 96 12:06:42 EST
Subject: re: High resolution printer

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To: "'microscopy'" {Microscopy-at-Sparc5.Microscopy.Com}
Cc:

To: "'microscopy'" {Microscopy-at-Sparc5.Microscopy.Com}
Cc:

To: {microscopy-at-Sparc5.Microscopy.Com}
Cc:

I am interested in receiving the summary from you. Thanks in advance.

________________________________
Yuhui Xu,MD,PhD
Core EM Facility, DFCI




From: Glen MacDonald :      glenmac-at-deskmail.washington.edu
Date: Fri, 13 Dec 1996 09:19:19 -0200
Subject: Re: TEM phosphor plates vs. CCD camera systems

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Can anyone provide the name of a US dealer for the phospor plates?

On Wed, 11 Dec 1996 12:21:26 -0500 Microbill-at-aol.com wrote:

} The phosphor plates have (potentially) a higher resolution than currently
} affordabel CCD devices. Actually, at the last price (I haven't seen the
} latest plate prices) I saw for the plate system you could probably afford a
} 2kx2k CCD camera.

TIA,
Glen MacDonald
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, Wa 98195-7923
(206) 616-4156
glenmac-at-u.washington.edu

~~~~~~~~~~~~~~~~~~~~~~~~
"The box said "Requires Windows 95, or better" so I bought a Macintosh."
~~~~~~~~~~~~~~~~~~~~~~~~





From: darrell_miles-at-vnet.ibm.com
Date: Fri, 13 Dec 96 10:59:10 EST
Subject: Re: Acrylate Allergies!

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Hi again,

(Please ignore my spelling typo's, it was late last night.)
I meant to mention that the Nitrilite gloves are lightweight surgical
type gloves, and very easy to work in.

Darrell Miles
darrell_miles-at-vnet.ibm.com

IBM Analytical and Test Services
Solutions for Industry
http://www.chips.ibm.com/services/asg/




From: Terry Mitchell :      tmitche-at-mst.lanl.gov
Date: Fri, 13 Dec 1996 10:42:32 -0700
Subject: EM Staff Position

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I sent a message out on October 23 saying that Los Alamos would "shortly"
be advertising an Electron Microscopy Staff Position in the Center for
Materials Science. That position is now finally official and can be found at

http://www.hr.lanl.gov/html/jobs/external/972168.job.html

We have already started to interview candidates but applications are still
accepted until the end of the year.

**********************************
* Terence E. Mitchell
* Laboratory Fellow
* Center for Materials Science
* Mail Stop K765
* Los Alamos National Laboratory
* Los Alamos, NM 87545
* Tel: 505-667-0938
* Fax: 505-665-2992
* E-mail: temitchell-at-lanl.gov
**********************************






From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Fri, 13 Dec 1996 12:42:09 -500
Subject: Re: Santovac 5

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O.k., kept under normal lab environmetal conditions (or reasonable
special conditions), i.e. "on the shelf", is there a recognized
"shelf life" for Santovac 5? Recognizing the abuse it receives in a
diff. pump I would think that its pretty damned stabile, but would
like a confirmation of my industrial chemistry ignorance.


Richard E. Edelmann
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu




From: Helen Campbell :      HELEN-at-MINMET.Lan.McGill.CA
Date: Fri, 13 Dec 1996 14:07:56 EST5EDT
Subject: RCPT: TEM powder prep - THANKS

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X-SMTP-Posting-Origin: lansend.cc.mcgill.ca (lansend.CC.McGill.CA [132.206.37.4])
Message-Id: {199612131916.OAA25462-at-sirocco.CC.McGill.CA}

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Confirmation of reading: your message -

Date: 12 Dec 96 17:29
To: microscopy-at-Sparc5.Microscopy.Com
Subject: TEM powder prep - THANKS

Was read at 14:07, 13 Dec 96.





From: kris-at-elod.vein.hu (Kris Kovacs)
Date: Fri, 13 Dec 1996 22:12:21 +0100
Subject: Grain size measurement of films

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Dear Microscopists,

I have to measure the grain size and shape of silver halide particles of
normal and high speed b/w films. Are there any tips and tricks on how to
remove the gelatine and expose the grains without disturbing their size/shape?

Thanks,

Kris





From: Sara Miller :      saram-at-acpub.duke.edu
Date: Fri, 13 Dec 1996 16:46:58 -0500 (EST)
Subject: Colloidal Gold Sources

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Dear List Members,

There has been a great deal of experience and opinion discussed recently
on the brands of colloidal gold. It seems to me that the conclusion from
all this is that there are a number of companies that are
making/marketing good products, as none of the comments here have
mentioned bad experiences with any. We have found that different
products have different shelf lives and differing size stringencies;
sometimes these variations are WITHIN THE SAME BRAND. Varying size
ranges can be a problem if you're trying to do multiple labelings. Short
shelf life can be circumvented by ordering small amounts (0.25 ml). The
one brand I endorsed previously always gives us good results, and in most
cases, the products last WAY beyond the expiration date. One course
would be to find one you like and stick with it to avoid having to test
reactivity on myriad products. However, do carry control positive and
negative samples in each experiment.

There are numerous companies that distribute these products, some of
which I mentioned previously. It appears that, in giving examples of a
few sellers, but not all, I have offended some by omission. Perhaps I
should have listed all the products on the market or none. At any rate, I
meant no offense in my deletions; my sincere apologies. To make amends,
I have tried to collect the names of the companies that I'm aware of that
sell immunogold commercially (The list may not be complete; if you know
of others, please feel free to add them). In no order of
preference--only alphabetical:

Amersham
2636 Clearbrook Dr.
Arlington Heights, IL 60005
800 323 9750

BB International - Goldmark Biologicals
437 Lock St.
Phillipsburg, NJ 08865
908 859 2631

BioRad Laboratories/Life science Group
200 Alfred Nobel Dr.
Hercules, CA 94547
510 741 1000

BioRad - Microscience Div.
19 Blackstone St.
Cambridge, MA 02139
800 444 1422

Chemicon International, Inc.
28835 Single Oak Dr.
Temecula, CA 92590
800 437 7500

Electron Microscopy Sciences
321 Morris Rd
Box 251
Fort Washington, PA 19034
800 523 5874

Energy Beam Sciences, Inc.
PO Box 468
11 Bowles Rd.
Agawam, Mass 01001
800 992 9037

Ernest F. Fullam, Inc.
900 Albany Shaker Rd.
Latham, NY 12110
518 785 5533

EY Laboratories
107 N. Amphlett Blvd.
San Mateo, CA 94901
800 821 0044

Jackson Immunoresearch Laboratories, Inc.
PO Box 9
872 W. Baltimore Pke.
West Grove, Pa 19390
800 367 5296

Ladd Catalog
PO Box 1005
Burlington, VT 05402
800 451 3406

Nanoprobes
25 East Loop Dr., #124
Stony Brook, NY 11790-3350
516 444 8815

Pierce Life Science & Analytical Research Products
3747 N. Meridian RD.
PO Box 117
Rockford, IL 61105
800 874 3723

Polysciences, Inc.
400 Valley Rd.
Warrington, PA 18976
800 523 2572

Sigma Chemical Co.
PO Box 14508
St. Louis, MO 63178
800 325 3010

SPI Supplies
PO Box 656
569 E. Gray St.
West Chester, PA 19381-0656
800 2424 SPI

Ted Pella, Inc.
4595 Mountain Lakes Blvd.
Redding, CA 96003
800 237 3526

And of course, one invaluable (well worth the $75) source of information
on antibodies in general is:
Linscott's Directory
PO Box 26221
Birmingham, AL 35260
800 766 7000

I am not associated in any way with these companies, and I make no
warranties on the accuracy of this information or the quality of the
products. If you sell gold and are not listed (sorry), feel free to add
your name to the hat.
Sara


Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: William Tivol :      tivol-at-wadsworth.org
Date: Fri, 13 Dec 1996 16:48:05 -0500 (EST)
Subject: Image plates vs CCD

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Dear Lucille,

} What are the advantages/disadvantages (including price) in using the
} reusable TEM phosphor plates vs. a dedicated CCD camera system for
} collecting images?
}
One disadvantage of image plates vs CCD is that the IP's are not
nearly as sensitive at high voltage. The quantum efficiency drops to ~20%
at 1250 kV--there was a poster at MSA which had that info. This may be of
no interest to you unless you have an HVEM, as I do.
Yours,
Bill Tivol




From: yuhui xu :      Yuhui=Xu%RES%DFCI-at-EYE.DFCI.HARVARD.EDU
Date: Fri, 13 Dec 96 18:05:54 EST
Subject: EM Tech Position Open

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The Core Electron Microscopy Facility at Dana Farber Cancer Institute
located in Boston, Massachusetts has a position immediately open for a full
time EM Technician to assist in daily operation of the central research
facility. Requirements include: B.S. or equivalent in life sciences; one to
two years experience working as an EM technician; a strong background in
transmission and scanning electron microscopy and associated preparative
techniques, including tissue processing, Epoxy resin embedding, ultrathin
sectioning and contrast staining, immunogold staining, and dark room
procedure. Computer knowledge including word processing and data base
programs is required. Cryoultramicrotomy skill is desirable but not
required. Office management skills including filing, ordering and billing are
also helpful. M-F, 40h/W, Salary $20-25K/year with excellent benefit. DFCI is
an equal opportunity Employer. If interested, please send by email resume to
Yuhui Xu, MD,PhD, at the following address: Yuhui_Xu-at-dfci.harvard.edu. No
phone inquiries please.








From: kszaruba-at-MMM.COM (Karen S. Zaruba)
Date: Fri, 13 Dec 1996 18:23:39 -0600
Subject: TEM of skin

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I have made three attempts to process human cadaver skin for TEM with no
success. Each time, the stratum corneum is separated from the rest of the
epidermis, sometimes in sheets.

I have fixed in glutaraldehyde or paraformaldehyde, dehydrated in acetone or
ethanol, and embedded in LR White or Spurr's. So far I have not even made it
to the TEM because the separation is so evident on my LM slides.

Any suggestions?? Your help is appreciated,

Karen Zaruba


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
3M Company
3M Center 270-1S-01 Phone: 612-737-2971
St. Paul, MN 55144-1000 Fax: 736-1519

These opinions are my own and may not represent those of 3M.







From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Fri, 13 Dec 96 23:04:40 -0500
Subject: Shelf life stability of Santovac 5

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Message-Id: {199612140350.WAA25577-at-ns1.axs2000.net}
To: MICROSCOPY BB {Microscopy-at-Sparc5.Microscopy.Com}

-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Richard Edelman wrote:
=========================================
O.k., kept under normal lab environmetal conditions (or reasonable special
conditions), i.e. "on the shelf", is there a recognized "shelf life" for
Santovac 5?
=========================================

Over the years, I have had the chance to visit customers and distributors in
tropical kinds of settings, some of which have had Santovac 5 (tm) sitting
on the shelf, in unopened bottles, and in non-airconditioned environments
for years. While I can recall seeing some of the labels starting to come
off, never ever have I ever heard from anyone of an instance of "old"
Santovac 5 being inferior to "new" Santovac 5. I am unaware of any kind of
guarantee from the manufacturer that would indeed be the case, but from my
perspective, there are few things in our laboratories as stable long term as
Santovac 5 sitting on a shelf.

Chuck

======================================================
Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400
President 1-(800)-2424-SPI
SPI SUPPLIES FAX: 1-(610)-436-5755
PO BOX 656 e-mail: cgarber-at-2spi.com
West Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com


Look for us!
############################
WWW: http://www.2spi.com
############################
=====================================================




From: Francisco J. Hernandez :      fjhblasq-at-biomed.icb2.usp.br
Date: Sun, 15 Dec 1996 10:30:19 -0200 (EDT)
Subject: DNA quantitation and ploidia: cs-200 software

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We are stimulating the grow of liver cells (hepatocytes)
in vivo with hepatotrofic factors and we would like
to know if the ploidia is being affected. That is, we
would like to know if the cells are diploid, triploid or
tetraploid by quantifying the DNA in nuclei of cells in
histologic sections.
Somebody has told me that there is a programa named
CAS-200 (DNA QUANTITATION SOFTWARE) that may differentiate
between diploid, triploid and tetraploid cells.

Does anyone know how the program works?
The same kind of measurements may be done with another
program like BioScan Optimas, or SigmaScan?

I would be very grateful for any help
=============================================================================
Francisco Javier Hernandez Blazquez |
Universidade de Sao Paulo - Faculdade de | e-mail fjhblazq-at-spider.usp.br
Zootecnia e Engenharia de Alimentos | Voice: 55 19 5618606 r. 229
Departamento de Ciencias Basicas/Histologia| 55 19 5618887
Av. Duque de Caxias Norte, 225 CP 23 | Fax: 55 19 5611689
CEP 13630-000 Pirassununga (Sao Paulo) |
BRAZIL |
==============================================================================






From: Keyur Shah :      kshah-at-uscom.com
Date: Sun, 15 Dec 1996 09:00:40 -0500
Subject: Mast cells

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Does any one have any experience in running up the mast cells for
Immunocytochem or In Situ at EM level? Since I cannot osmicate the
tissue(so far ran up skin, ear lobe or tongue from an albino mice) the
subsequent steps of dehydration extracts the granules and makes it
difficult to identify the cells. Also the holes make it impossible to do
insitu with.
Any help and suggestions will be highly appreciated.
Thanks in advance. and Seasons Greetings...
N. Shah

visit us at...
http://www.med.upenn.edu/~path/core/EMCMAIN1.HTM




From: Hand-at-nso1.uchc.edu (Hand,Arthur)
Date: Sun, 15 Dec 1996 15:23:55 -0500
Subject: Registered: Hand,Arthur

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DATE: 12/12/96 18:05
TO: Hand,Arthur
SUBJECT: Optical Scopes

Was accessed on 12/15/96 at 15:17





From: Hand-at-nso1.uchc.edu (Hand,Arthur)
Date: Sun, 15 Dec 1996 15:24:02 -0500
Subject: Registered: Hand,Arthur

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DATE: 12/12/96 22:30
TO: Hand,Arthur
SUBJECT: Optical Scopes

Was accessed on 12/15/96 at 15:17





From: Hand-at-nso1.uchc.edu (Hand,Arthur)
Date: Sun, 15 Dec 1996 15:23:23 -0500
Subject: Registered: Hand,Arthur

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DATE: 12/12/96 21:30
TO: Hand,Arthur
SUBJECT: Unwanted messages

Was accessed on 12/15/96 at 15:16





From: oshel-at-ux1.cso.uiuc.edu (Philip Oshel)
Date: Sun, 15 Dec 1996 16:51:20 -0600
Subject: Re: Optical Scopes

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} I'm thinking of buying a new low power ( {50x) zoom OM for general
} inspection work prior to SEM work.
}
} If you were going to buy a scope today, what would you get? What would
} you avoid? What accessories would you get (i.e. ring lights, diffuse
} lighting, polarzing filters, etc.)?
}
} I'd like only user input, no sales pitches.
} Thanks
} -------------------------------------------------
} Harold J. Crossman

Harold,
Personally, I'd look at Olympus and Nikon first. I did notice at
the Society meeting in Mpls that Zeiss has an inexpensive new
stereoscope (which they credited to a new automated factory). This one has
a clever way of giving dark-field without popping an extra $2000 or so for
a special stage. The Russian LOMO would be worth a look, but I've heard
varying things about the quality.
Accessories will depend on use (such as the dark field I
mentioned)--what do you need to do? For polarizing filters, I'd try for
some way to mount camera filters, the ones that fit on 35mm SLRs. Lighting
will be most critical. Get a multihead fiber optic--2 heads at least--if
you can get 2 2-head units. A fiber optic ring light will be useful if you
need shadowless illumination. Cameras: get a photo tube even if you don't
plan on taking pictures, you'll want to eventually. Don't spend money on
fancy photomicroscopy cameras, meters, etc. I've found that an used Olympus
OM4 back works fine, and it's metering gives excellent exposures (just make
sure the meter is working right).
Phil

&&& Illigitimi non carborundum &&&&&&&&
Philip Oshel
Microscopy
Station A
PO Box 5037
Champaign, IL 61825-5037
(217)244-3145 days
(217)355-3145 evenings
oshel-at-ux1.cso.uiuc.edu
*** looking for a job again ******************







From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Sun, 15 Dec 1996 17:00:00 -0500
Subject: Re: collagen - TEM

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Message-Id: {v03007802aeda250349d9-at-[206.69.208.21]}
In-Reply-To: {SIMEON.9612132006.B-at-drk.shcc.org}
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Doug

The last few posting that the Microscopy Listserver has from
you have consistently occurred twice. Are you intentionally
sending 2 copies? I'm trying to sort out things at this end and
need to see if it is a system problem or something that you
are inadvertently doing.

Thanks for your help

Nestor
Microscopy SysOP


} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America







From: Nestor J. Zaluzec :      zaluzec-at-Sparc5.Microscopy.Com
Date: Sun, 15 Dec 1996 17:21:50 -0500
Subject: Another System Wide Message from Nestor

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Message-Id: {v03007804aeda26fdc0ad-at-[206.69.208.21]}
Mime-Version: 1.0
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Well folks,

The survey that I sent out had no systematic
clues as to why some of you received messageless
banners and some did not. There was no real
consistent pattern of OS, Email Program or whatever.
Except that most of you didn't really know what
your Host Email system was running.

Anyway I've not gotten any NEW reports of
messages only containing banners for the last
day. (BTW this message should have NO BANNER).

There have been a number of "Return Receipt"
messages/reports. These occur when one of two thing
happens.

First, you have checked an option in your Email
program which embeds a hidden command in your
Email message to notify the you when the person
you have sent the message reads, deletes, or receives
the message. PLEASE check you system. If you have
such an option TURN IT OFF. The Listserver becomes the
owner of all messages to the system, hence return
receipts loop right back to the system. Those of you
who have been on this system long enough will remember
the disaster we had about a year ago when a message
setup with automatic reply created an infinite loop.

Secondly, some programs (very few) also have options
which all them to inform the sender that a message
has been received, deleted, read etc... If you have'
that option also please turn it off.

I have send message to those individuals that I
can identify whose computers have started posting
the problem messages and have temporairly removed
them from the Master Database. When they fix their
Email Program then I will reinstate them.

Hopefully this will cure the latest set of problems that
have cropped up.

Please continue to report to me any problems with
mail dated from Monday 12/16/96.

Do Not bother to report problems with messages dated
before then as I will already have those on record.

If you are still receiving messageless banners
on 12/16 please also report it directly to me.

Zaluzec-at-microscopy.com



Later....

Nestor
Your Friendly Neighborhood SysOp







From: Melvyn Dickson :      M.Dickson-at-unsw.edu.au
Date: Mon, 16 Dec 1996 10:32:40 +1100
Subject: Re: Optical Scopes - Macro option

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Message-Id: {1.5.4.32.19961215233240.00ad83fc-at-pop3.unsw.edu.au}
X-Sender: s7001031-at-pop3.unsw.edu.au
X-Mailer: Windows Eudora Light Version 1.5.4 (32)
Mime-Version: 1.0
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I chose to get a Leica M420 Macroscope for this precise purpose. We Had a
good stereo microscope (Wild) which I used for years. Good optics for
stereo viewing but when we wanted images the off axis nature of the images
was very obvious. The Macroscope works in the same magnification range but
with on-axis optics the recorded images are superior. I bought the achromat
objective but there is a apochromat available for top performance. I did
not buy the photo system but put a CCD B/W video camera on a C mount on the
macroscope.

No connection with Leitz, just happy with my choice.

Mel Dickson
Director,
E.M. Unit, UNSW





From: Stephen Edgar :      s.edgar-at-auckland.ac.nz
Date: Mon, 16 Dec 1996 14:28:08 +1300 NZDT
Subject: SEM: Very low mag on ISI DS-130

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Season's Greetings everyone,

This is a question for users of the DS-130 model of SEM made by ISI
(an oldie but goodie).

Does anyone know how to use the "Objective Lens" and "Scan 2"
controls to get very low magnification, low distortion images? The
manual implies that you simply turn both of these controls to the
"off" position, but if I do that I get a very small field of view
(because of the objective aperture silhouette) and an image which
cannot be focused. It is quite low mag though. Perhaps I should
mention that I use the obj aperture intended for the first stage
in second stage mode also, because the image is much brighter that
way (i.e. the aperture at the bottom of the column has been removed).
Even if that is the cause of my aperture silhouette problem, I am
still left unable to focus. Any ideas would be welcomed...



Regards

Stephen Edgar

Electron Microscope Unit, Pathology Department
School of Medicine
University of Auckland
Private Bag 92019
Auckland
New Zealand

email address: s.edgar-at-auckland.ac.nz
Phone : +64-9-3737599 extn 6473 (GMT + 12h)
Fax : +64-9-3737459




From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin)
Date: Sun, 15 Dec 1996 20:44:48 -0500
Subject: data analysis/spline fit program

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Greetings,
Anyone out there know of good software for fitting splines to
data? At present, we are struggling with SAS, which works, but from which
it is tricky (!) to extract the relevant coefficients. Note, we need to fit
a smooth curve through data for which we do not have a model function,
hence our interest in splines. We don't just want to drive a spline curve
through each data point, which is all that a couple of programs we have
checked can do.
I realize that this is not explitly a biophysics or microscopy
question, but as the our data have been generated from image analysis
through a microscope, I hope I may be forgiven for supposing someone on
these lists could help.
Thanks,
Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
___ ____ ^ ____ _____ Tobias I. Baskin
/ \ / / \ / \ / 1623 University Ave.
/ | / / \ / / Columbia, Missouri
/___ / /__ /_____\ / /__ 65201 USA
/ / / \ ( /
/ / / \ \ / voice: 573 - 443-1984
/ /____ / \ \____/ /_____ fax: 573 - 882-0123






From: Mike Witcomb :      MIKEW-at-gecko.biol.wits.ac.za
Date: Mon, 16 Dec 1996 12:06:04 GMT+2
Subject: NiSi standards for EDS

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Seasons greetings to everyone.

I wonder if anyone knows a source, person or organisation, that would
have a NiSi EDS standard for the SEM in the composition range 10-
50at%Ni (about 20-75wt% Ni) - a sample in the range 10-20 at%Ni would
be great.

Mike


Michael J Witcomb PhD
Electron Microscope Unit
University of the Witwatersrand
Private Bag 3
WITS
2050
South Africa

Telephone: + 27 11 716 4000
+ 27 11 716 2419 (messages)
Fax: + 27 11 339 3407
E-mail: mikew-at-gecko.biol.wits.ac.za





From: Rudolf Fuchshofer :      sz1270-at-max3.rrze.uni-erlangen.de
Date: Mon, 16 Dec 1996 11:28:29 +0000
Subject: Fix-algae

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I need to fix, stain and embed Dinoflagellates.
I'm looking for specific fixations, staining and dehydration advice.

I would thank you for any advice.

Rudolf Fuchshofer
Uni-Erlangen, institute zoology I
E-Mail: sz1270-at-stud.uni-erlangen.de




From: Owen P. Mills :      opmills-at-mtu.edu
Date: Mon, 16 Dec 1996 09:21:57 -0500
Subject: SF6 gas

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Hello,

It may be necessary to open the HV tank on our JEOL 4000. In doing so we
will have to replace the sodium hexafluoride insulation gas (no, not oil)
that resides there. Have any of you with this type of tank had to buy SF6
lately? Can anyone recommend a distributor that currently has this product
in stock?

FYI, SF6 is about $4,000 per standard cylinder and hard to locate. Also, it
is a consumable and therefore not covered by service contract!!!

There goes the new image analysis software package....

Dear Santa, all I want for Christmas is some SF6....

Happy holidays,
Owen
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: csedax-at-alpha.arcride.edu.ar
Date: Mon, 16 Dec 1996 12:37:55 -2359
Subject: Problems EDAX

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To: "'microscopy'" {Microscopy-at-Sparc5.Microscopy.Com}
Cc:

To: {microscopy-at-Sparc5.Microscopy.Com}
Cc:

To: "'microscopy'" {Microscopy-at-Sparc5.Microscopy.Com}
Cc:

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Dear microscopists,

this week we've had problems calibrating our EDAX
PV9100, ECON II, system attached to our SEM.

We usually use a Cu-Al stub for calibrating at two peaks: Al Ka and Cu ka.
We situate the sample in such a way that we see on the screen half area Al and
half area Cu in order to get both peaks with about the same height.

This week we had the following problem: we calibrated like always but, this
time the Cu Ka shifted on the energy axis to about 3.3 keV. Of course, the Al
peak was totally missed. Such a shift we had never seen.

By adjusting the Zero, the Gain and the Fast discrimator settings at the
preamplifier we could, after trying for a afternoon, register the Al peak and
calibrate both peaks at the right energy.

The problems that remains unsolved is the height of the peaks. As we get
the equal areas (Cu and Al) on the screen, we now get a Cu peak which is almost
25% of the height of the Al one. What does it mean? Are we getting some of
the Cu signals cut by the preamplifier ?

Our electronic technicians have no idea about it's going on. Does anyone
have any suggestions about what the problem is? If so, could you please comment
them to us? We would appreciate it very much.

Thanks in advance.
Silvia Montoro
Centro Regional de Investigacion y
Desarrollo de Santa Fe
Santa Fe - Argentina
csedax-at-arcride.edu.ar















From: cook-at-aaem.amc.anl.gov (Russell E. Cook)
Date: Mon, 16 Dec 1996 09:48:14 -0600
Subject: Re: SF6 gas

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Owen Mills asked about SF6 gas cylinders.

We get ours through AGA. Large cylinders cost us about $500, not $4000!
Try calling AGA's main office in Ohio for a local distributer: (419)
893-7226.

Russell E. Cook
Electron Microscopy Center for Materials Research
Argonne National Laboratory
9700 South Cass Avenue
MSD-212
Argonne, IL 60439
(630)252-7194
FAX: (630)252-4798






From: Neal Nicklaus :      nnicklaus-at-nova.sarnoff.com
Date: Mon, 16 Dec 1996 12:05:03 -0500
Subject: Light Microscopy with Colloidal Gold

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Has anyone been looking at the scattering from colloidal gold particles as
positional tags on proteins?

I am interested in tagging enzymes and tracking their location in a
conventional research microscope by tagging them with ~1-5 nm gold
particles. I understand from second-hand sources, and from a few abstracts
I have seen (but have not yet looked at the articles), that ~4 nm gold
particles may be seen just from their scattering properties in phase
contrast or DIC mode.

I have one article (J of Microscopy, Vol 173, Pt 1, Jan 1994, pp27-38) that
demonstrates that 1 nm gold particles may be viewed in reflection mode using
a confocal microscope. Does anyone know what size gold particle might be
viewed in a conventional microscope in perhaps dark field reflection mode?




Neal Nicklaus
Senior Scientist
SEQ Limited

Voice: 609-452-6033 Ext. 13
Fax: 609-452-5955
email nnicklaus-at-seq.sarnoff.com





From: Robert Underwood :      underwoo-at-u.washington.edu
Date: Mon, 16 Dec 1996 09:12:33 -0800 (PST)
Subject: Re: TEM of skin

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Hello,

We work on human skin. Is the cadaver skin fixed in formaldehyde before
you get it? If so, the ultrastructure will suffer. An excellent fixtive to
use is half strength karnovsky and embed in epon. LR White has the strange
problem of not polymerizing where the tissue is filaggrin rich, which is
right at the transition cell layer, thus the stratum corneum has trouble
staying on.
Bob
Morphology Core
U of Washington

On Fri, 13 Dec 1996, Karen S. Zaruba wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} I have made three attempts to process human cadaver skin for TEM with no
} success. Each time, the stratum corneum is separated from the rest of the
} epidermis, sometimes in sheets.
}
} I have fixed in glutaraldehyde or paraformaldehyde, dehydrated in acetone or
} ethanol, and embedded in LR White or Spurr's. So far I have not even made it
} to the TEM because the separation is so evident on my LM slides.
}
} Any suggestions?? Your help is appreciated,
}
} Karen Zaruba
}
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
} 3M Company
} 3M Center 270-1S-01 Phone: 612-737-2971
} St. Paul, MN 55144-1000 Fax: 736-1519
}
} These opinions are my own and may not represent those of 3M.
}
}
}
}






From: hartnell-at-biomed.med.yale.edu
Date: Mon, 16 Dec 1996 12:32:23 -0500 (EST)
Subject: Re: TEM of skin

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{Pine.PMDF.3.91.961216094654.221954A-100000-100000-100000-100000-100000-100000-100000-100000-at-BIOMED.MED.YALE.EDU}
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From: hartnell-at-biomed.med.yale.edu
Date: Mon, 16 Dec 1996 12:32:23 -0500 (EST)
Subject: Re: TEM of skin

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Dear Karen,

Here is a trick which has worked for retina. Try embedding the skin in
gelatin first, even before it is fixed. This should help to hold the
layers together during subsequent manipulations and further processing.

If fixation is done before you receive the tissue, the gelatin may still
prevent your sample from separating as much. Good luck and keep us
posted.

Lisa Hartnell, Res. Associate
Yale University School of Medicine
Center for Cell Imaging
Hartnell-at-biomed.med.yale.edu

On Fri, 13 Dec 1996, Karen S. Zaruba
wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} I have made three attempts to process human cadaver skin for TEM with no
} success. Each time, the stratum corneum is separated from the rest of the
} epidermis, sometimes in sheets.
}
} I have fixed in glutaraldehyde or paraformaldehyde, dehydrated in acetone or
} ethanol, and embedded in LR White or Spurr's. So far I have not even made it
} to the TEM because the separation is so evident on my LM slides.
}
} Any suggestions?? Your help is appreciated,
}
} Karen Zaruba
}
}
} ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
} Life Sciences Sector Lab Reply: kszaruba-at-mmm.com
} 3M Company
} 3M Center 270-1S-01 Phone: 612-737-2971
} St. Paul, MN 55144-1000 Fax: 736-1519
}
} These opinions are my own and may not represent those of 3M.
}
}
}
}










From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: Mon, 16 Dec 1996 10:23:52 -0800
Subject: Sputter Targets

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To: Microscopy messageslistserver {Microscopy-at-Sparc5.Microscopy.Com}

-- [ From: Nancy A. Monteiro-Riviere, Ph.D. * EMC.Ver #2.5.02 ] --


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Hi Everyone;

I have a general question that may be obvious to some, but is
not obvious to me. When is a sputter coater target "spent",
and require replacement? Does it simply no longer coat the
sample effectively, or is it something more subtle? In the case of a
blended target (mine is 60:40 Au/Pd), does one element deplete before
the other? I have had the same target for several years now.

TIA,

-Bob
*******************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA 91711
USA
Ph: (909)399-1311
Email: Bob_Citron-at-cc.chiron.com
*******************************




From: Burke, Mike :      wx-burkema-at-westinghouse.com
Date: Mon, 16 Dec 1996 15:37:00 -0500
Subject: Position Opening

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Westinghouse Science and Technology Center Pittsburgh Pa has an opening
for a physical metallurgist/electron microscopist to provide TEM/AEM
support for the metallurgy group. The materials to be studied are
mainly nuclear and fossil based power generation materials. For more
details contact :
Mike Burke
Manager Metallurgy Section
Westinghouse Science and Technology Center
Pittsburgh Pa

Phone : 412-256-1788
Fax : 412-256-1221
EMAIL : mx-burkema-at-westinghouse.com




From: fams-at-holonet.net
Date: Tue, 17 Dec 1996 15:11:43 +0400
Subject: SCANNING 97

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Program information for SCANNING 97 is now posted on the SCANNING page,
www.scanning-fams.org

Sessions and speakers will be updated weekly.







From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 16 Dec 1996 17:20:30 -0400
Subject: RE-EDAX CalibProb

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"Micros/PkHts" {microscopy-at-Sparc5.Microscopy.Com}
X-Mailer: Mail*Link SMTP/QM 3.0.0GM



From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 16 Dec 1996 17:20:30 -0400
Subject: RE-EDAX CalibProb

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Subject: Time: 5:05 PM
OFFICE MEMO RE:EDAX CalibProb Date: 12/16/96

One thing that can cause relative peak heights to vary is the electron
accelerating voltage being used. If you normally have been working around
20 kV and then drop down to a relatively low operating kV, say 12 kV, you may
get relatively inefficient excitation of the Cu atoms (because Eo {2Ec),
whereas the Al atoms will be efficiently excited (because Eo} 5Ec) and this
can cause the Al peak to be smaller than the Cu peak (the Cu L peak will
also be larger than the CuK peak). The old 'rule of thumb' was to choose an
accelerating voltage that is 2 to 3 times the critical excitation voltage of
the highest energy line being used in the analysis.





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 16 Dec 1996 17:24:00 -0400
Subject: RE-CalibProb-Corr'td

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"Micros/PkHt" {microscopy-at-Sparc5.Microscopy.Com} ,
"Micros/PkHts" {microscopy-at-Sparc5.Microscopy.Com}
X-Mailer: Mail*Link SMTP/QM 3.0.0GM



From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 16 Dec 1996 17:24:00 -0400
Subject: RE-CalibProb-Corr'td

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Subject: Time: 5:05 PM
OFFICE MEMO RE:CalibProb-Corr'td Date: 12/16/96

Sorry to send this again, but I made a typo in the first version and typed
'smaller' instead of 'LARGER": see below ;

One thing that can cause relative peak heights to vary is the electron
accelerating voltage being used. If you normally have been working around
20 kV and then drop down to a relatively low operating kV, say 12 kV, you may
get relatively inefficient excitation of the Cu atoms (because Eo {2Ec),
whereas the Al atoms will be efficiently excited (because Eo} 5Ec) and this
can cause the Al peak to be LARGER than the Cu peak (the Cu L peak will also
be larger than the CuK peak). The old 'rule of thumb' was to choose an
accelerating voltage that is 2 to 3 times the critical excitation voltage of
the highest energy line being used in the analysis.





From: Kirk Rogers :      rogers-at-er6.eng.ohio-state.edu
Date: Mon, 16 Dec 1996 16:03:17 -0500
Subject: Re: data analysis/spline fit program

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microscopy {Microscopy-at-Sparc5.Microscopy.Com}
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From: Kirk Rogers :      rogers-at-er6.eng.ohio-state.edu
Date: Mon, 16 Dec 1996 16:03:17 -0500
Subject: Re: data analysis/spline fit program

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} Anyone out there know of good software for fitting splines to
} data? At present, we are struggling with SAS, which works, but from
} which
} it is tricky (!) to extract the relevant coefficients.

Sounds like a trick for either MathCad {http://www.mathsoft.com} or
Mathematica {http://www.wolfram.com} , although Igor Pro might be a good
choice {http://www.wavemetrics.com} . I personally Prefer MathCad for my
data analysis, because equations are written and executed just like you
would expect (i.e. as you would write them out on paper). Good luck!

-Kirk
___________________________________________________
No Window of Opportunity: Upgrading from Windows 3.1 to Windows 95 or
NT is not as cheap as some might have thought. The Gartner Group Inc.,
found that it takes two to three days per desktop to do the
conversion. This translates into thousands of dollars and countless
new headaches.

- Client/Server Computing, August '96
___________________________________________________
This message was created and sent using the Cyberdog Mail System
___________________________________________________
Kirk Rogers E: rogers-at-er6.eng.ohio-state.edu
Office: (614) 688-4067 FAX: (614) 292-1537
Materials Science and Engineering The Ohio State University
477 Watts Hall, 2041 College Ave. Columbus, OH 43210








From: Kirk Rogers :      rogers-at-er6.eng.ohio-state.edu
Date: Mon, 16 Dec 1996 16:03:17 -0500
Subject: Re: data analysis/spline fit program

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microscopy {Microscopy-at-Sparc5.Microscopy.Com}
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From: Kirk Rogers :      rogers-at-er6.eng.ohio-state.edu
Date: Mon, 16 Dec 1996 16:03:17 -0500
Subject: Re: data analysis/spline fit program

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} Anyone out there know of good software for fitting splines to
} data? At present, we are struggling with SAS, which works, but from
} which
} it is tricky (!) to extract the relevant coefficients.

Sounds like a trick for either MathCad {http://www.mathsoft.com} or
Mathematica {http://www.wolfram.com} , although Igor Pro might be a good
choice {http://www.wavemetrics.com} . I personally Prefer MathCad for my
data analysis, because equations are written and executed just like you
would expect (i.e. as you would write them out on paper). Good luck!

-Kirk
___________________________________________________
No Window of Opportunity: Upgrading from Windows 3.1 to Windows 95 or
NT is not as cheap as some might have thought. The Gartner Group Inc.,
found that it takes two to three days per desktop to do the
conversion. This translates into thousands of dollars and countless
new headaches.

- Client/Server Computing, August '96
___________________________________________________
This message was created and sent using the Cyberdog Mail System
___________________________________________________
Kirk Rogers E: rogers-at-er6.eng.ohio-state.edu
Office: (614) 688-4067 FAX: (614) 292-1537
Materials Science and Engineering The Ohio State University
477 Watts Hall, 2041 College Ave. Columbus, OH 43210








From: Xiaogang Xie :      xxie-at-LSUVM.SNCC.LSU.EDU
Date: Mon, 16 Dec 1996 16:53:13 -0600
Subject: Re: SEM: Very low mag on ISI DS-130

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Back a while ago, I acquired a few low-mag images for some of our std discs.
I believe it is the PROBE-CURRENT knobs can be used for focusing the low mag
image (may called SPOT-SIZE, CONDENSER, or RESOLUTION on yours?). This is
done under electron chenneling pattern observation. Hope it helps.

Xiaogang

***********************************
* Xiaogang Xie *
* SEM & Microprobe lab *
* Dept. of Geology and Geophysics *
* Louisiana State University *
* Baton Rouge, LA 70803 *
* Office (504)388-2240 *
* Fax (504)388-2302 *
***********************************





From: Microscopy-request
Date: Monday, December 16, 1996 9:21AM
Subject: SF6 gas

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Owen:

We get our SF6 from Scott Specialty Gases in NJ, but they might have
offices in your area. Their number is (908)754-7700. We use it in our
JEM2010 and we order VLSI grade. I believe it is also called instrument
grade (99.995 purity).

Jordi Marti
AlliedSignal
----------

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Hello,

It may be necessary to open the HV tank on our JEOL 4000. In doing so we
will have to replace the sodium hexafluoride insulation gas (no, not oil)
that resides there. Have any of you with this type of tank had to buy SF6
lately? Can anyone recommend a distributor that currently has this product
in stock?

FYI, SF6 is about $4,000 per standard cylinder and hard to locate. Also, it
is a consumable and therefore not covered by service contract!!!

There goes the new image analysis software package....

Dear Santa, all I want for Christmas is some SF6....

Happy holidays,
Owen
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: Cubdrvr-at-aol.com
Date: Mon, 16 Dec 1996 19:46:51 -0500
Subject: Re: Sputter Targets

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In a message dated 96-12-16 19:01:35 EST, Bob_Citron-at-cc.chiron.com (Bob
Citron) writes:

} When is a sputter coater target "spent",
} and require replacement? Does it simply no longer coat the
} sample effectively, or is it something more subtle? In the case of a
} blended target (mine is 60:40 Au/Pd), does one element deplete before
} the other?

Typically (at least for the small foil types used in small EM-type coaters),
it's spent when you sputter a hole in it. Take it out of the holder and hold
it up to the light- if you can see little pinholes, it's time. If you leave
it too long, you'll start sputtering the face of the cathode.

If the target is bonded, you'll start to sputter the substrate when it burns
through, this may be harder to detect by visually examining the target. I
have no experience w/ bonded targets.

As for a blended target, the ratio shouldn't change as it is used.

Best.

Pat




From: Mary Mager :      mager-at-unixg.ubc.ca
Date: Mon, 16 Dec 1996 21:03:39 -0800
Subject: Re: Problems EDAX

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Message-Id: {2.2.32.19961217050339.00688834-at-pop.unixg.ubc.ca}
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Dear Silvia Montoro:
You wrote:
...
} This week we had the following problem: we calibrated like always but, this
} time the Cu Ka shifted on the energy axis to about 3.3 keV. Of course, the Al
} peak was totally missed. Such a shift we had never seen.
}
The only time I had such a drastic shift in peaks was when my Kevex 8000 got
a broken wire in the high-voltage bias supply. The broken wire was under the
heat-shrink behind the BNC connector, so it couldn't be seen. The bias
degrades slowly, so the peaks were gradually going lower and broader. This
bias is difficult to test accurately because it is high voltage but almost
no current, so check your manuals carefully.
Luck,
Mary
Mary Mager
Electron Microscopist
Metals and Materials Eng., UBC
6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel:604-822-5648, fax:604-822-3619
e-mail: mager-at-unixg.ubc.ca





From: David Dryden :      djd-at-physics.unimelb.EDU.AU
Date: Tue, 17 Dec 1996 16:13:41 +1100
Subject: Australian list server.

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Could someone please forward me the email address for the
Australian EM email list server.

David Dryden
School OF Physics
Uni OF Melbourne





From: Dr P. Echlin :      pe13-at-cus.cam.ac.uk
Date: Tue, 17 Dec 1996 08:54:23 +0000 (GMT)
Subject: Re: Sputter Targets

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Dear Bob:

It is my understanding that gold and palladium sputter congruently
unlike evaporation where the two metals are deposited as a function of
their melting point. You could check this by depositing some Au-Pd and
checking the ratio br XRMA. We consider a target is worn out when holes
begin to appear. We don't seem to get much joy in re-cycling target
material.

Seasons greetings from Cambridge UK

Patrick Echlin
Multi-Imaging Centre
Scool of Biological Sciences.

On Mon, 16 Dec 1996, Bob Citron wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Hi Everyone;
}
} I have a general question that may be obvious to some, but is
} not obvious to me. When is a sputter coater target "spent",
} and require replacement? Does it simply no longer coat the
} sample effectively, or is it something more subtle? In the case of a
} blended target (mine is 60:40 Au/Pd), does one element deplete before
} the other? I have had the same target for several years now.
}
} TIA,
}
} -Bob
} *******************************
} Bob Citron
} Chiron Vision
} 555 W. Arrow Hwy
} Claremont, CA 91711
} USA
} Ph: (909)399-1311
} Email: Bob_Citron-at-cc.chiron.com
} *******************************
}





From: Vladimir.Oleshko :      oleshko-at-uia.ua.ac.be
Date: Tue, 17 Dec 1996 11:45:12 +0100 (MET)
Subject: Re: Grain size measurement of films

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On Fri, 13 Dec 1996, Kris Kovacs wrote:
}
} Dear Microscopists,
}
} I have to measure the grain size and shape of silver halide particles of
} normal and high speed b/w films. Are there any tips and tricks on how to
} remove the gelatine and expose the grains without disturbing their size/shape?
}
} Thanks,
}
} Kris

Dear Kris,

You can release silver halide particles from b/w films by an enzymatic
hydrolysis of gelatin in a saturated solution of Pronasa E for 45-60 min
at t=38-40oC following by weak centrifugation (2,000 rpm) for 10 min. It
is necessary to resuspend the deposit several times and to wash thoroughly
with distilled water following by the centrifugation to remove gelatin.
Separated grains may be coated on thin films (TEM) or on slides (SEM). In
order to avoid silver halide decomposition under the beam, one can use
cooling at LN2 temperature and/or one-step carbon replicas.

For the production of replicas a carbon film should be evaporated onto
particles deposited on glass slides in a vacuum unit at about 10-3 Pa.
The carbon film may be separated from the support by a treatment with 2-5
% aqueous HF solution following washing with distilled water to remove
traces of acid, dissolving the grains in a 10% sodium thiosulfate solution
and washing again with distilled water. Afterwards, the film should be
coated on the grids and dried.

Sometimes, b/w films, in particular, high speed ones may have a composite
arrangement which comprises high-sensitive and low-sensitive semilayers
with emulsions of different sizes and shapes. Therefore, ultramicrotomy
(TEM) and cryofractography (SEM) of films is also useful and grains may be
released from different semilayers using a layer-by-layer hydrolysis of
gelatin by the choose of an appropriate time of the treatment. Some
references and examples of such studies you can find in our review published
in Microbeam Analysis, 4, (1995), 1-29.

Regards,

----------------------------------------
Vladimir Oleshko, Ph.D.
University of Antwerp (UIA)
Chemistry Department
Micro- and Trace Analysis Centre (MiTAC)
Universiteitsplein, 1
B-2610 Antwerpen-Wilrijk, Belgium
Tel.: +32-3-820.23.64
Fax: +32-3-820.23.76
e-mail: oleshko-at-uia.ua.ac.be
----------------------------------------




From: makroczy-at-ccsun.tuke.sk
Date: Tue, 17 Dec 96 11:02:25 GMT
Subject: Discussion about SF6 gas

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To: Microscopy-at-Sparc5.Microscopy.Com (Microscopy Listserver U.S.A.)

Hello,

I have noticed, that the discussion about SF6 gas opened on Listserv. I have
another question. I heard that it is possible to replace Freon gas in TEM Jeol
2000FX by SF6 gas without changing the electron gun (SF6 needs higher pressure
than Freon), only some attachment is necessary for joining the SF6 tank to
microscope Freon system. Does anybody know about supplier of SF6 gas in Europe,
who can provide this service in Slovak Republic?

Thank you

Yours sincerely

Peter Makroczy
makroczy-at-ccsun.tuke.sk
----
__________________________________________________________
Peter MAKROCZY
Technical University of Kosice
Department of Materials Science
Park Komenskeho 11
040 01 Kosice
SLOVAK REPUBLIC

E-mail: makroczy-at-ccsun.tuke.sk
Tel.: ++42 +095 63 335 49
Fax.: ++42 +095 63 327 23
__________________________________________________________







From: lklfalk-at-fy.chalmers.se (Lena Falk)
Date: Tue, 17 Dec 1996 14:21:09 +0100
Subject: Analytical TEM post-doc position

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POST-DOCTORAL POSITION:
"Analytical Electron Microscopy of Oxynitride Glass Ceramics"

Division for Microscopy and Microanalysis
Chalmers University of Technology
S-412 96 G=F6teborg
SWEDEN

A post-doctoral position is available within the framework of the=
Training
and Mobility of Researchers (TMR) Programme. Qualified candidates=
will
have a Ph.D. in materials science, chemistry of physics as well as
documented experience with analytical electron microscopy. The candidate
must be a national of a Member State of the European Community.

The research project, "Structure and Properties of New M-Si-Al-O-N
Oxynitride Glass Ceramics (M =3D Y, Ln)", is a collaboration between=
seven
partners in the United Kingdom, Ireland, France, Sweden and Belgium.=
The
aim of the project is to determine structures and properties of currently
uncharacterized glass-ceramics in yttrium and rare earth sialon systems.=
=20
These materials are potential refractory grain boundary phases in
Si3N4-based ceramics, and have an interst also as refractory surface
coatings (glazes) and as interface materials in nitride-oxide and
nitride-metal joints.

The work carried out at Chalmers will focus on the chemistry and=
structure
of glasses and their crystallisation products, and on microstructural
development during nucleation and growth processes. The major part=
of the
microscopy will be carried out on a Philips CM200 SuperTwin TEM equipped
with a field emission gun (FEG), the Gatan imaging filter (GIF) and=
the
Link Isis EDX system.

The post-doctoral researcher will initially be appointed for one=
year with
a possibility to prolong the appointment. The maximum duration is=
2.5
years, and the project has to be finished by the end of December=
1999. The
starting date of the appointment can be discussed, and the starting=
salary
is around 3.394 ECU per month.

The collaboration within the established network will give the
post-doctoral researcher good contacts with a number of research=
groups in
Europe and a good knowledge of a variety of preparative / analytical=
/
property measurement techniques relevant to the evaluation of new=
ceramics
as well as other materials. The work will involve visits to the=
other
partners in the network.

=46or futher details, please contact:

Dr. Lena K.L. Falk,
Division for Microscopy and Microanalysis, Department of=
Physics,
Chalmers
University of Technology, S-412 96 G=F6teborg, SWEDEN. =
=20
=
=20
e-mail: lklfalk-at-fy.chalmers.se; fax: + 46 31 772 3224;=
=20
phone: + 46 31 772 3321






From: Frank Thomas :      thomasf-at-AGC.BIO.NS.CA
Date: Tue, 17 Dec 1996 08:30:28 -0400 (AST)
Subject: Electromagnetic Fields & Health

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In the past there have been a number of interesting threads on this
listserver regarding various health issues of importance to electron
microscopists (e.g. do SEM's cause cataracts, etc.).
While I'm generally not a fanatic about these sorts of items and
tend not to worry about them, I thought I should bring up an interesting
conversation I had in a bar the other night. I was talking to this guy, who,
when he found out I run an SEM lab, told me that for several years he had
worked in an office with two other gentlemen which had been situated directly
behind an SEM lab. This would have been more or less from about 1980 to
1990, give or take a year.
Now for the interesting part. He told me that so far the other two
fellows have had, between them, three hip replacement operations, with a
fourth pending, and that my informant was also beginning to have problems
with his hips, and had already found that his athletic activities were beginning
to be curtailed. ( All three of the guys in question, I might add, are
stated to be now just in their late forties or fifties.)
He had, of course, become concerned and had been looking for some common
factor in their work environment which may have affected all three of them,
and the only thing he could come up with was the proximity of their
workstations to the SEM on the other side of the wall. Three out of three
is, after all, an impressive statistic, even in a small sample, when you're
one of them.
My informant had been informally researching SEM's in the past few weeks,
and was aware that X-ray emissions are generally not an issue with them, but
was becoming increasingly interested in possible electromagnetic fields
associated with these instruments. He told me of research which tends to
suggest that longterm exposure to EM fields may have serious effects on bone
joints, so if SEM's do indeed create strong local EM fields, there may be
some connection.
So, I wonder.... Do you suppose that there may be anything to this? Do
people with longterm associations with SEM labs exhibit higher than average
incidences of bone/joint disorders? For that matter, do SEMs create EM
fields capable of affecting people, say, ten feet away on the other side of
a wall?
Now I don't generally jump on every health scare bandwagon that comes
along, but this fellow appeared to be a reasonably well-educated and
intelligent guy (and never once mentioned aliens) so I thought I'd throw his
story out to the listserver and see what comes of it.
And Season's Greetings, by the way.
F.C. Thomas
GSC (Atlantic) MicroAnalysis Facility
P.O. Box 1006, Dartmouth, N.S.
B2Y 4A2
(902) 426-4635




From: Woody.N.White%650 :      Woody.N.White-at-mcdermott.com
Date: Tue, 17 Dec 1996 8:10:00 -0500
Subject: Re: Problems EDAX

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A failure of the pulse processor (typically, the ADC) can also cause
apparent shifts in peak energies. The frist thing (and easiest) to
check are the power supply volatges and especially the ADC reference
voltages. _Woody_




From: Craig, Bob :      craig-at-OSI.SYLVANIA.com
Date: Tue, 17 Dec 1996 08:16:50 -0500
Subject: Re: Electromagnetic Fields and Health

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Frank Thomas recently raised the question regarding EM fields associated
with SEMs and health.

My direct experience with electron optical instruments goes back to 1970
and lasted 23 years. This included a daily dose of whatever fields were
associated with an EPMA and SEM. During this period I have suffered no
health problems other than the usual occupational hazards of working in
the dark, i.e., fungus and moss and the distinct personality of a troll.
The only adverse affects I am aware that electron microscopists have
are related to the ergonomics of operating the instruments, i.e.,
repetitive motion syndrome, back strain, eye strain, etc.

In my opinion the hip deterioration described raises more questions
about the quality of the drinking water that any field environment. I
would assume the average circuit breaker panel would have a higher
electromagnetic field than an SEM.

Looking forward to the opinions and experiences of others.

Bob Craig
OSRAM SYLVANIA INC.




From: Barbara Miner, TEMlab 17-Dec-1996 1036 :      miner-at-asdg.enet.dec.com
Date: Tue, 17 Dec 96 10:36:09 EST
Subject: Re: EM fields and health

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We did a survey for EM fields in my TEM (300kV) lab (HRSEM in the next lab).
The highest field in the room was associated with my color monitor for the EDX
system. Second highest source was the fluorescent lights.

B.Miner





From: ebs-at-ebsciences.com
Date: Tue, 17 Dec 1996 10:03:25 -0600
Subject: Sputter Targets

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Hi Bob & fellow microscopists!

Let me start by introducing myself, I am the Business manager for the
POLARON range of preparation equipment including coaters. Your
question to the target dilemma is simple, but interesting one.

The most simple answer is that when the target becomes perforated
then it is deemed to be spent. BUT, depending on the backing metal it
is possible to continue sputtering with this same target for some
time.

What happens is that the target area that is being bombarded is
reduced so the sputter rate is continually in decline, if the coater
has a sufficiently strong Plasma then the backing metal will be
sputtered at a slower rate but will contaminate the gold or target
material. Not a problem for visualisation of the surface by SE
detector.

When the target is of a mixed metal composition then the softer (Less
dense) material will erode quicker, in practice this is not
significant as the mixed metal is used to stop conglomeration and
island formation of the sputtered material (normally gold) hence
offering higher resolution or more correctly, smaller grain size.

Our recommendation for reproducible high quality work is to change
the target once perforated.

There is much more that we could discuss but I hope this answers your
question.

Best regards - Tony

Regards,

Tony King
Product specialist
VG Microtech/ Polaron range

Tel: +44 (0)1825 746251
Fax: +44 (0)1825 768343

E&OE
********************************
Energy Beam Sciences, Inc.
Adding Brilliance To Your Vision
ebs-at-ebsciences.com
http://www.ebsciences.com/
********************************





From: A.M.Schmid :      bt3221-at-qmw.ac.uk
Date: Tue, 17 Dec 1996 16:11:00 -0800
Subject: Staining of Sucrose in Thin EM (Lowicryl embedded) Sections

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Has anybody a good technique (or reference) to stain for sucrose in
moss-tissue, pressure-frozen, freeze substituted and Lowicryl embedded
thin sections?
Please?
My email address is A.M.Schmid-at-qmw.ac.uk




From: wise-at-vaxa.cis.uwosh.edu
Date: Tue, 17 Dec 1996 10:21:02 +0000
Subject: SEM chamber scope

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I'm interested in pricing a chamber scope (scope and monitor and
whatever accessories are needed) for a Hitachi 2460N. Does anyone out
there in the yelm of microscopy have any recommendations?
Please respond directly to me and not the list.

Thanks,

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-uwosh.edu






From: wise-at-vaxa.cis.uwosh.edu
Date: Tue, 17 Dec 1996 10:17:41 +0000
Subject: image capture from SEM

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To all,

Portions of this subject have been discussed over the past few
months but I haven't really been paying close attention to it. I apologize
in advance if we rehash ground already covered.
We have a (an?) Hitachi 2460N SEM and I'm looking into outfitting
it for digital image capture (with annotation, file storage, printer, etc).
I know that Hitachi sells a system that is supposed to plug right in and
comes with a lot of bells and whistles. Has anyone had any experience with
the Hitachi system? Alternatively, does anyone know of an after market
product that performs well? Given that I am a computer dummy, I would be
more inclined to purchase a total system rather than attempt to put one
together on my own from individual components.
Please respond directly to me and not to the list.

Thanks in advance,

Bob


Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-uwosh.edu






From: Owen P. Mills :      opmills-at-mtu.edu
Date: Tue, 17 Dec 1996 12:14:36 -0500
Subject: hourly rates

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Hello,

A colleague of mine is investigating hourly rates charged for XRD & XRF. I
believe many of you may have this instrumentation in or near your EM labs.
Will you contact me off line with any info on hourly rates for that type of
instrument. Thanks.

Owen
Owen P. Mills
Michigan Technological University
Metallurgical & Materials Engineering
Rm 512 MME Building
Houghton, MI 49931
906-487-2002
906-487-2934 FAX
opmills-at-mtu.edu





From: Anthony Garratt-Reed :      tonygr-at-MIT.EDU
Date: Tue, 17 Dec 1996 13:03:18 -0500
Subject: Re: Problems EDAX

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I saw similar symptoms once in a detector on an SEM. We warmed and pumped
the detector and it then worked again. I believe we first checked the
detector on another analyzer we had available, so we knew the problem was in
the detector/preamp area.

Tony.





****************************************************
****************************************************
** **
** Anthony J. Garratt-Reed **
** Room 13-1027 **
** Center for Materials Science and Engineering **
** Massachusetts Institute of Technology **
** 77 Massachusetts Avenue **
** Cambridge, Massachsetts 02139-4307 **
** U. S. A. **
** **
** Phone: 617-253-4622 **
** Fax: 617-258-5286 or 617-258-6478 **
** **
****************************************************
****************************************************






From: robert herrmann :      rah1-at-acpub.duke.edu
Date: Tue, 17 Dec 1996 10:27:49 -0500 (EST)
Subject: sectioning through metal stents

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Hi Netters,
=09Recently, there was a discussion on sectioning metal stents which=20
I didn't save because we don't do them. However, I have just received=20
this message from a former student. Can anyone help him? Thanks.
Sara

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710=20
Ph: 919 684-3452
FAX: 919 684-8735

---------- Forwarded message ----------

Dear Dr. Miller,

I'm not sure if you remember me. I'm the biomedical engineering grad
student that took your EM class several years ago. I just graduated in
September with the PhD. I'm in Munich Germany now doing some post doctoral
work on the stenting or coronary arteries.

I'm writing to you to ask if you have any infomation regarding the section
of tissue that contain small pieces of metal. The stent that we are
researching is made of stainless steel and we would like to cut some type
of plastic embedded sections at around 4 =B5m thick, remove the plastic and
do some immunohistochemistry. We do have a published report where they do
just that, but we have tried to use the polymethylmethacrylate and it
produces artifacts for use. It looks like the plastic is not as hard as
the steel and the cutting knife (tungsten carbide?) pushed the small steel
piece, producing an artifact in the tissue next to the metal.

Do you know of any plastics that are harder than PMMA or anyone I could
contact that might be of any assistance. I know this is not much
information, but I thought you might be able to help.

Thanks for you assistance,
Have a Merry Christmas

Robert Herrmann
rah1-at-acpub.duke.edu

Robert Herrmann, Ph.D.
Klinikum rechts der Isar
Munchen, Germany
rah1-at-acpub.duke.edu






From: Scott Whittaker :      sdw-at-biotech.ufl.edu
Date: Tue, 17 Dec 1996 15:22:14 -0500
Subject: Re: image capture from SEM

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Hi Bob. I maintain an archive of most of the biologically relavant
postings to this list. You are correct that this has been a hot topic in the
last few months and I have archive it at our web site. Go to the URL listed
at the end of this message and click on the "Tips & Tricks" link. Within
there you will find a section on "Computers" Look at the section titles
"Acquiring Digital Images". Let me know and I can get you the info in some
other way if you need. Good Luck




} To all,
}
} Portions of this subject have been discussed over the past few
} months but I haven't really been paying close attention to it. I apologize
} in advance if we rehash ground already covered.
} We have a (an?) Hitachi 2460N SEM and I'm looking into outfitting
} it for digital image capture (with annotation, file storage, printer, etc).
} I know that Hitachi sells a system that is supposed to plug right in and
} comes with a lot of bells and whistles. Has anyone had any experience with
} the Hitachi system? Alternatively, does anyone know of an after market
} product that performs well? Given that I am a computer dummy, I would be
} more inclined to purchase a total system rather than attempt to put one
} together on my own from individual components.
} Please respond directly to me and not to the list.
}
}




} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {} {
GO GATORS
Scott D. Whittaker 218 Carr Hall
Research Assistant Gainesville, FL 32610
University Of Florida ph 352-392-1295
ICBR EM Core Lab fax 352-846-0251
sdw-at-biotech.ufl.edu http://www.biotech.ufl.edu/~emcl/
The home of " Tips & Tricks "





"To do the honors of a table gracefully, is one of the out-lines of a
well-bred man;
and to carve well, little as it may seem, is useful twice every day, and
the doing of
which ill is not only troublesome to ourselves, but renders us disagreeable apt
ridiculous to others."

Reverend John Trusler, 1788, quoting Lord Chesterfield.





From: Craig -at- Evex :      ctheberge-at-evex.com
Date: Tue, 17 Dec 1996 15:48:09 -0500
Subject: subscribe

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{microscopy-at-Sparc5.Microscopy.Com}

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subscribe





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 17 Dec 1996 16:33:22 -0400
Subject: RE-Health & Mag Fields

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Subject: Time: 4:09 PM
OFFICE MEMO RE:Health & Mag Fields Date: 12/17/96

We just had a survey of the magnetic fields run on our two SEM labs, one
with an Hitachi S-800 and the other with an Hitachi S-520, in each case with
the instrument in operation. Nowhere in either lab was there a field
greater than a fraction of a milligauss (specs for the S-800 require less
than 0.5mG).
If the SEMs in your labs are performing anywhere near to specifications
it is likely that the fields in your labs are of about the same magnitude.
The strength of fields of this kind fall off as the third power of distance
[i.e. B = f(1/r^3)], and so it seems highly unlikely that any fields
produced in the SEM lab would make a measurable contribution in an adjoining
office. In fact, it is quite likely that the fluorescent lights in a
typical office, or normal ground currents through the structural members of
the building, would produce higher fields.
Statistically, it is quite possible for highly unlikely events (such as
three people having joint trouble) to occur. The frequency with which this
happens is usually quite low, however, and so such events are commonly called
miracles or catastrophies, depending on the perspective from which we view
them. [Have these guys been getting plenty of calcium (1200 mg/day),
magnesium (200 mg/day) and vitamin D in their diets?]






From: yuhui xu :      Yuhui=Xu%RES%DFCI-at-EYE.DFCI.HARVARD.EDU
Date: Tue, 17 Dec 96 17:11:55 EST
Subject: Just a test

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Sorry to post this message because I have not received any mail since this
Monday. This is just a test.




From: yuhui xu :      Yuhui=Xu%RES%DFCI-at-EYE.DFCI.HARVARD.EDU
Date: Tue, 17 Dec 96 17:33:07 EST
Subject: Job posting

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Due to the problems either from our network or our computer, I have not
received any messages from this listserver for two days. Therefore I will
appreciate it very much if anyone of you who responded to my job posting( EM
Tech at Dana Farber Cancer Institute) re-send your response to me. Sorry for
the inconvenience.

Yuhui Xu, MD,PhD
DFCI




From: Jacky Larnould :      larnould-at-mnet.fr
Date: Tue, 17 Dec 1996 23:45:28 +0100
Subject: Re: Electromagnetic Fields and Health

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At 08:16 17/12/1996 -0500, you wrote:
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From: Jacky Larnould :      larnould-at-mnet.fr
Date: Tue, 17 Dec 1996 23:45:32 +0100
Subject: Re: Discussion about SF6 gas

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At 11:02 17/12/1996 GMT, you wrote:
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a 400 Kv.
Concerning the 2000FX or EX it's not so easy just increasing the pressure in
gun and what about the HV Tank?
And all the safety devices has been set for Freon Gas. So it's necessary to
readjust them.
Hope the discussion will continue.
Greetings.
==========================================================
Jacky Larnould
tel 33 (0)4 67 72 28 26
fax 33 (0)4 67 79 54 90
email larnould-at-mnet.fr





From: Melvyn Dickson :      M.Dickson-at-unsw.edu.au
Date: Wed, 18 Dec 1996 10:45:06 +1100
Subject: Re:SEM Electromagnetic Fields & Health

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I have made many measurements of Magnetic field strength around our SEMs.
They do not radiate large fields. They have metal shielding around the
columns to keep fields OUT and these and the iron shrouds in the lens
casings very effectively keep fields IN. Also the heavy lens currents are
DC. The AC current driving the scan coils is realtively small. Any fields
that escape from the system fall below ambient at half a metre (say 2 feet)
from the column. The rest of the system is also designed so strong fields
are not radiated to interfere with the microscope.

There are far stonger sources of ELF AC fields around our building, giving a
general ELF AC background of 10-20 milligauss. In fact it is hard these
days to locate any area with a field less than 3 milligauss, even in the
open air.

I am unconvinced that ELF AC fields are harmful to health. But I am sure
that the popular cosmetic appearance of a sun tan is a radiation burn which
can lead to skin cancer. Pick your own risk!

Mel Dickson





From: Bob_Citron-at-cc.chiron.com (Bob Citron)
Date: Tue, 17 Dec 1996 16:57:13 -0800
Subject: Sputter Targets - Thanks

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Fellow Microscopists;

Many thanks to all who responded to my questions about sputter
targets. I pulled mine off and examined it for pitting (as you
suggested). It's 5 years old and still good...

-Bob
****************************
Bob Citron
Chiron Vision
555 W. Arrow Hwy
Claremont, CA 91711
USA
(909)399-1311
Bob_Citron-at-cc.chiron.com
****************************




From: Jorge A. Santiago-Blay :      blay-at-pondside.uchicago.edu
Date: Tue, 17 Dec 1996 19:35:16 -0600
Subject: Removing cuticular hydrocarbons and keeping specimens alive!

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Hi:

Problem: Need to remove cuticular hydrocarbons from flies and have them
alive after the trauma! Any suggestions?

Thanks.

Wish to summarize responses if enough interest. Let me know if OK to
acknowledge your replies (as I like to do but, once in a while one
stumble across people who wish their remarks not be attached to their
id, thus my request for permission).





From: Warren Straszheim :      wes-at-ameslab.gov
Date: Tue, 17 Dec 1996 17:30:27 -0600
Subject: Re: RE-Health & Mag Fields

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At 04:33 PM 12/17/96 -0400, you wrote:
} The strength of fields of this kind fall off as the third power of distance
} [i.e. B = f(1/r^3)],

I have heard this a few times now, but can someone tell me why it is the 3rd
power? I thought the energy flux or intensity fell off as 1/r**2 from a
point source. The area over which the energy is spread increases with r**2.
For a line source the fall-off was 1/r, while for a plane source there was
no fall-off.

Can you physics majors out there explain this to me. I know enough EE to be
dangerous. Is it that magnetic field strength is different?
----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Mihaly Kozma :      KOZMA-at-anat-fm.szote.u-szeged.hu
Date: Wed, 18 Dec 1996 08:58:21
Subject: RCPT: TEM powder prep - THANKS

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Confirmation of reading: your message -

Date: 12 Dec 96 17:29
To: microscopy-at-Sparc5.Microscopy.Com
Subject: TEM powder prep - THANKS

Was read at 8:58, 18 Dec 96.





From: Mihaly Kozma :      KOZMA-at-anat-fm.szote.u-szeged.hu
Date: Wed, 18 Dec 1996 09:08:33
Subject: RCPT: TEM powder prep - THANKS

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Confirmation of reading: your message -

Date: 12 Dec 96 17:29
To: microscopy-at-Sparc5.Microscopy.Com
Subject: TEM powder prep - THANKS

Was read at 9:08, 18 Dec 96.





From: Stephan Coetzee :      stephan-at-gecko.biol.wits.ac.za
Date: Wed, 18 Dec 1996 12:41:23 GMT+2
Subject: Texbook SEM Biological

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Dear all

A quick question. We do have Rhodin's Histology Text and Atlas
which serves us well. Is there anything similar available for SEM
sample identification?

Thanks and Seasons Greetings to all
*
****
******
***********
[][]

##
[########]
##
##
##
##
##
Stephan H Coeztee
Electron Microscope Unit
Private Bag 3
Wits
2050
South Africa

Stephan-at-Gecko.biol.WITS.ac.za

Tell: +27 11 716 2419
Fax : +27 11 339 3407




From: Richard E. Edelmann :      edelmare-at-CASMAIL.MUOHIO.EDU
Date: Wed, 18 Dec 1996 08:23:30 -500
Subject: More Sputtering on targets.

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Since there is a current discussion on targets, I have become very
curious regarding those people who get an incredible 5yrs + out of
their targets. Now granted there are differing rates of usage, and
differing deposition amounts in various labs and users, but it would
seem that target thickness is one of the major factors in this
longevity.

I have a Denton Desk II, which takes a disk target (2.375"
diameter). Looking through the catalogs and talking with a few
other EM labs, targets vary from 0.0015" to 0.01" thick. Since we
are generally talking about Au or Au/Pd electrical conductivity/
resistance through the target shouldn't be a major concern (?). Is
there any procedures, other than trial and error, for determining
how thick a target one can use? Or even more specifically can anyone
give me the answer for a Denton Desk II ?


Note: It is not my intent to support nor condem in any way any
sputter coater vendor.



Richard E. Edelmann, Ph.D.
Electron Microscopy Facility Supervisor
352 Pearson Hall
Miami University, Oxford, OH 45056
Ph: 513-529-5712 Fax: 513-529-4243
E-mail: edelmare-at-muohio.edu




From: A. Kent Christensen :      akc-at-umich.edu
Date: Wed, 18 Dec 1996 08:43:48 -0500 (EST)
Subject: Re: Texbook SEM Biological

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Stephan,

Kessel RG, Shih CY, 1973. Scanning Electron Microscopy in Biology: A
Student's Atlas on Biological Organization. Springer-Verlag (New York),
345 pp.

Motta P, Andrews PM, Porter KR, 1977. Microanatomy of Cell and Tissue
Surfaces: An Atlas of Scanning Electron Microscopy. Lea & Febiger
(Philadelphia).

Kent

A. Kent Christensen,
University of Michigan
{akc-at-umich.edu}

------------------------

On Wed, 18 Dec 1996, Stephan Coetzee wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} Dear all
}
} A quick question. We do have Rhodin's Histology Text and Atlas
} which serves us well. Is there anything similar available for SEM
} sample identification?





From: lkerr-at-mbl.edu (Louis Kerr)
Date: Wed, 18 Dec 1996 09:12:18 -0500
Subject: Re: Electromagnetic Fields & Health

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I just read an article in a local paper (originally from the LA Times)
linking EMF exposure to Alzheimer's incidence. It references an article in
today's issue of the journal Neurology, authored by Dr. Eugene Sobel of
USC. The most startling statement was "the greatest risk was for people
who operate sewing machines". The theory is that the exposure is high
because they work so close to the electrical motor in the machine. Another
high risk group were carpenters and others who use electrically power tools
held close to the body.

I think it helps to show that there is still much debate on the hazards of
exposure to EMF fields and that less obvious, mundane activities could pose
serious risks.

Louie Kerr

Louie Kerr
Research and Education Support Coordinator
Marine Biological Laboratory
7 MBL Street
Woods Hole, MA 02543
508-289-7273
508-540-6902 (FAX)

VISIT OUR WEB SITE:
http://www.mbl.edu






From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Wed, 18 Dec 1996 09:56:11 -0500 (EST)
Subject: DAB Precipitate

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Yuck! We have DAB precip on our immunocytochemistry sections. They are
Araldite embed sections on slides that we have etched and circled with PAP
pen. Has anyone else had this problem and do you have any solutions? Is
it possible to get the precip off once it has formed? We haven't had any
luck. Any suggestions would be greatly appreciated.

Cheri Owen
Detroit Neurotrauma Institute
Wayne State University
Detroit, Michigan
(313)577-4648





From: SveEn-at-pai.liu.se (Sverker Enestrom)
Date: Wed, 18 Dec 1996 15:51:41 +0100
Subject: Re: Texbook SEM Biological

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} A quick question. We do have Rhodin's Histology Text and Atlas
} which serves us well. Is there anything similar available for SEM
} sample identification?
}
Greetings,
Johannes Rohdin's Histology is from 1974 and a SEM atlas I know
is from 1979: Kessel RG and Kardon RH: Tissues and Organs, a text-atlas
of scanning electron microscopy. W.H. Freeman and Company, San Francisco
ISBN 0-7167-0091-3
Best regards.
Sverker

*********************************************************
Sverker Enestrom M.D., Ph.D.
Department of Pathology
University of Linkoping, Sweden
Phone: +46 13 22 15 20
Fax: +46 13 13 22 57
*********************************************************






From: Joseph M. Oparowski, DTN 225-6538, HLO2-3/J09 :      oparowski-at-asdg.enet.dec.com
Date: Wed, 18 Dec 96 09:53:28 EST
Subject: FE-SEM with WDX

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Message-Id: {9612181453.AA23208-at-easynet.crl.dec.com}

Greetings,

We currently employ an EPMA/SEM system for the analysis of semiconductors
and packaging materials. Features on these materials are often less than
one micrometer in size, which necessitates the use of low accelerating
voltages to minimize the interaction volume. This leads to EDXS peak
overlap situations, especially for N Ka/TiLa and SiKa/W Ma pairs.
The use of WDX has been most beneficial for resolving these peak
overlaps. However, our current LaB6 column lacks the resolution to
adequately image submicron features and films.

We have considered replacing the LaB6 system with a field emission SEM
equipped with a WDX spectrometer. Several vendors have demonstrated the
ability of their FE guns to produce stable beam currents in excess of
15nA, which should be sufficient for WDX analyses. I would like to hear
from any users of FE-SEM/WDX systems as to their experiences and possible
recommendations. Thanks in advance.

Joseph M. Oparowski
Digital Equipment Corporation
77 Reed Road, HLO2-3/J09
Hudson, MA 01749-2895
joseph.oparowski-at-hlo.mts.dec.com




From: jeharper-at-amoco.com
Date: Wed, 18 Dec 96 08:58:43 -0600
Subject: Re: Sputter Targets - Thanks

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Now the profit motive...I have more than an ounce of gold targets that
I just can bear the thought of throwing in the trash can. Anybody
ever tried to sell the spent targets?

Jim Harper
jeharper-at-amoco.com





From: Robert Schmitz, Biology :      rschmitz-at-macsrv1.uwsp.edu
Date: Wed, 18 Dec 96 09:07:02 +0600
Subject: Re: Texbook SEM Biological

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} From: "Stephan Coetzee" {stephan-at-gecko.biol.wits.ac.za}
} To: microscopy-at-Sparc5.Microscopy.Com
} Date: Wed, 18 Dec 1996 12:41:23 GMT+2
} Subject: Texbook SEM Biological
}

} Dear all=20
}
} A quick question. We do have Rhodin's Histology Text and Atlas=20
} which serves us well. Is there anything similar available for SEM=20
} sample identification?

Is Rhodin's atlas is still in print in South Africa, I can't get it here in=
the =20
US, it is out of print. For SEM are you aware of Richard G. Kessel and Ran=
dt H.=20
Kardon 'TISSUES AND ORGANS: A TEXT-ATLAS OFSCANNING ELECTRON MICROSCOPY, Fr=
eeman=20
ISBN 0-7167-0090-5
}
} Thanks and Seasons Greetings to all
} ## =20
} Stephan H Coeztee
} Electron Microscope Unit
} Private Bag 3
} Wits
} 2050 =20
} South Africa
}
} Stephan-at-Gecko.biol.WITS.ac.za =20
}
} Tell: +27 11 716 2419
} Fax : +27 11 339 3407

rschmitz-at-uwspmail.uwsp.edu
or
rschmitz-at-macsrv1.uwsp.edu=20
(note its macsrv"one" not "el")
Robert (Bob) J. Schmitz
Department of Biology,=20
University of Wisc. Stevens Point.
Stevens Point, Wisconsin 54481
ph 715-346-2420




From: crd4-at-cornell.edu (Curt Dunnam)
Date: Wed, 18 Dec 1996 10:37:15 -0500 (EST)
Subject: Re: RE-Health & Mag Fields

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} At 04:33 PM 12/17/96 -0400, you wrote:
} } The strength of fields of this kind fall off as the third power of distance
} } [i.e. B = f(1/r^3)],
}
} I have heard this a few times now, but can someone tell me why it is the 3rd
} power? I thought the energy flux or intensity fell off as 1/r**2 from a
} point source. The area over which the energy is spread increases with r**2.
} For a line source the fall-off was 1/r, while for a plane source there was
} no fall-off.
}
} Can you physics majors out there explain this to me. I know enough EE to be
} dangerous. Is it that magnetic field strength is different?
} ----------------------------------------------------
} Warren E. Straszheim


All-

Common EMF sources are almost exclusively monopole, dipole or
quadrupole. The following analysis is technically 2-D since, among other
reasons, a 3-D monopole is rather difficult to model. Fortunately, in all
real-life EMF situations physical symmetry of the source in one or more
dimensions allows 2-dimensional analysis.

Monopole magnetic field sources are the most pervasive and
troublesome, and are typically due to stray currents where one part of the
current loop is relatively near and the remaining segments of the loop are
relatively far away. EMF distance-dependence for these cases is very close
to 1/r.

Dipole magnetic field sources are those where the source and return
current paths are in close proximity to each other with respect to the
observer's distance. Such sources include well-isolated (i.e., not much
leakage current) single- and three-phase transmission and distribution
circuits. Scaling is independent of the physical size of the circuit (big
transmission lines and home wiring are both in this category). EMF
distance-dependence for this class of sources is the familiar 1/r^^2
relationship.

Quadrupole sources include transformers, fluorescent ballasts,
motors and other devices such as VDT yoke electromagnets. Due to the
presence of physically alternating multiple flux paths (which,
incidentally, are only roughly predictable), these devices can emit locally
strong fields, but the fields decrease rapidly with distance. The common
EMF distance-dependence for these sources is 1/r^^3.

Higher-order components (hextupole, octopole, etc.) are often
present, but drop off with correspondingly higher inverse powers and are
typically inconsequential with regard to EMF's.


Best regards,
Curt Dunnam/C.U.






From: JUKNALIS :      juknalis-at-arserrc.gov
Date: Wed, 18 Dec 1996 11:08:02 -0500 (EST)
Subject: XYZ stage control

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I'm looking for a computer controlled XYZ stage control to attach
to an inverted Nikon diaphot. Mac & IPlab compatablity are a plus.
I already have info on the Ludl system. Anyone else have something different?

Thanks for any info you can forward me.

Joe Uknalis
juknalis-at-arserrc.gov
ARS-USDA





From: D. Reinebeck :      rreinebe-at-DIRECT.CA
Date: Wed, 18 Dec 1996 09:50:35 -0800
Subject: Microscope for sale

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Message-ID: {32B82EEA.7B04-at-DIRECT.CA}
Place-at-aphex.direct.ca, North-at-aphex.direct.ca,
Vancouver-at-aphex.direct.ca, British-at-aphex.direct.ca,
Columbia-at-aphex.direct.ca
X-Mailer: Mozilla 3.0 (Win16; I)
MIME-Version: 1.0
To: microscopy-at-Sparc5.Microscopy.Com

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I have a Carl Zeiss Photomicroscope I for sale. Asking price US$4,500.00
--
Dick Reinebeck, rreinebe-at-DIRECT.CA
2310 Magnussen Place,
North Vancouver, B.C.
V7J 3R6
(604) 984-6205




From: colijn.1-at-osu.edu (Henk Colijn)
Date: Wed, 18 Dec 1996 13:06:02 -0500
Subject: Re: RE-Health & Mag Fields

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{snip}
} Monopole magnetic field sources are the most pervasive and
} troublesome, and are typically due to stray currents where one part of the
} current loop is relatively near and the remaining segments of the loop are
} relatively far away. EMF distance-dependence for these cases is very close
} to 1/r.

I was a little puzzled by this at first, so I went back and checked my old
copy of Lorrain and Corson. For a "single long straight conductor", B does
indeed fall off as 1/r and a dipole has a 1/r^2 field dependence. A dipole
would be something like a solenoid (single or many turn) where you have
distinct "North/South" ends.

Power lines usually have 2 (or more) conductors. So you can consider a
power line to be a distorted (long and thin) solenoid loop and hence a
dipole radiator. Intuitively, as you get farther away from it, the power
line begins to look not like 2 wires, but like a single conductor with a
low net current. (i.e. the field from each conductor tends to cancel other
out, giving you a better than a 1/r dependence).


}
} Dipole magnetic field sources are those where the source and return
} current paths are in close proximity to each other with respect to the
} observer's distance. Such sources include well-isolated (i.e., not much
} leakage current) single- and three-phase transmission and distribution
} circuits. Scaling is independent of the physical size of the circuit (big
} transmission lines and home wiring are both in this category). EMF
} distance-dependence for this class of sources is the familiar 1/r^^2
} relationship.
}
{snip}
}
} Best regards,
} Curt Dunnam/C.U.

Cheers,
Henk Colijn

Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility
-------------------------------------------------------------------
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of the New. Francis Bacon, "Of Adversity."






From: psic-at-uclink4.berkeley.edu (Paula Sicurello)
Date: Wed, 18 Dec 1996 10:10:00 -0800 (PST)
Subject: Old JB4 resin

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Hello All!


I have some pretty old JB4 resin components that haven't been
refrigerated in ages. My question is...Can I catalyze this stuff and
polymerize it as if it were new? This stuff is a lot cheaper to dispose of
as a hard plastic than it is in it's basic components.
So, is it safe to do this? Or will I make the 6:00 news by doing this?


Happy Holidays!

Paula Sicurello
U.C. Berkeley
Elevtron Microscope Lab






From: Cheri Owen :      cowen-at-cmb.biosci.wayne.edu
Date: Wed, 18 Dec 1996 14:12:50 -0500 (EST)
Subject: Update:DAB Precipitate

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We just found that our DAB is precipitating when we add to our secondary.
Does anyone know how we can prevent this while still getting the DAB to
react with our peroxidase?

Cheri Owen
Detroit Neurotrauma Institute
Wayne State University
Detroit, Mi
(313)577-4648





From: Mark Wall :      Mark.Wall-at-quickmail.llnl.gov
Date: 18 Dec 1996 11:28:22 -0800
Subject: focused ion beam

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REGARDING focused ion beam

We have a Gatan PIMS (precision ion milling system, scanned focused probe of
Argon ions for etching selected regions) that we actually use from time to
time. I understand that Gatan sold several units with an upgraded liquid metal
ion source (LMS). Does anyone out there know who may have these units? Gatan
has not been able to give me this information. We are considering this upgrade
by purchasing a LMS from FEI. We believe that the lenses on the PIMS column
will not be needed but we are not sure if the PIMS deflection system will work
on these LM source of ions. Any comments are welcome. Either email of call.

Thanks in advance, happy holidays,

Mark A. Wall, L-350
Lawrence Livermore National Lab
7000 East Ave
Livermore, CA USA 94550
ph 510 423 7162
email mark.wall-at-quickmail.llnl.gov





From: colijn.1-at-osu.edu (Henk Colijn)
Date: Wed, 18 Dec 1996 15:06:35 -0500
Subject: Re: Sputter Targets - Thanks

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You might try contacting Abe Dayani at Refining Systems Inc. He was
willing to give us some credit on our old target.

Refining Systems, Inc.
P.O. Box 72466
Las Vegas, NV 89170

702 368-0579
702 368-0933 FAX

No connection other than as a customer.


Cheers,
Henk

}
} Now the profit motive...I have more than an ounce of gold targets that
} I just can bear the thought of throwing in the trash can. Anybody
} ever tried to sell the spent targets?
}
} Jim Harper
} jeharper-at-amoco.com

Henk Colijn colijn.1-at-osu.edu OSU Campus Electron Optics Facility
-------------------------------------------------------------------
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From: garyc-at-stud.ntnu.no (Gary)
Date: Wed, 18 Dec 1996 21:16:59 +0100 (MET)
Subject: 3D-software

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Hi!

I have been testing four different softwares for 3D reconstruction:
Voxelview
Voxblast
Spyglass
Macstereology

Does anyone have any experience with these software packages?. I did find
some publications about some of these programs, but it is not easy to get
them. I am wondering if somebody have publications that can be faxed to
me. I would pay if necessary.

Thanks!

/////////////////////////////////////////////////////////////////
// // //

// Gary Chinga // email :garyc-at-james.avh.unit.no //
// Plantebiosenteret // WWW :http://www.nvg.unit.no/~gary //
// NTNU, 7055 Dragvoll // phone : +47 73590168 //
// Norway // fax : +47 73590177 //
// // //
/////////////////////////////////////////////////////////////////






From: tom moninger :      moninger-at-emiris.iaf.uiowa.edu
Date: Wed, 18 Dec 1996 15:12:17 -0600 (CST)
Subject: Re: SEM chamber scope

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On Tue, 17 Dec 1996 wise-at-vaxa.cis.uwosh.edu wrote:

} ------------------------------------------------------------------------
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} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} I'm interested in pricing a chamber scope (scope and monitor and
} whatever accessories are needed) for a Hitachi 2460N. Does anyone out
} there in the yelm of microscopy have any recommendations?
} Please respond directly to me and not the list.
}
} Thanks,
}
} Bob
}
}
} Robert R. Wise, PhD
} Director, UWO Electron Microscope Facility
} Department of Biology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} (414) 424-3404 tel
} (414) 424-1101 fax
} wise-at-uwosh.edu
}
}

We have 2 of GW's infrared chanber cameras, one on a 2460-N. Both work
quite well--they have saved many a trainees samples (and the final lens
housing) from the bottom of the camber. About $3K I think. The image can
be integrated into the 2460's display.

Tom

Thomas Moninger (moninger-at-emiris.iaf.uiowa.edu)
Central Microscopy Research Facility http://www.uiowa.edu/~cemrf
85 EMRB University of Iowa
Iowa City, IA 52242 319-335-8142 FAX 319-335-8049





From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 18 Dec 1996 16:35:51 -0400
Subject: NRC on EMFs & Health

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"Micros/NRC Rpt" {miocroscopy-at-Sparc5.Microscopy.Com}
X-Mailer: Mail*Link SMTP/QM 3.0.0GM



From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 18 Dec 1996 16:35:51 -0400
Subject: NRC on EMFs & Health

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Subject: Time: 4:15 PM
OFFICE MEMO NRC on EMFs & Health Date: 12/18/96

As a final note on the possible relationship of magnetic fields to health
problems, I just noted the following comment in the January 1997 issue of
Scientific American (p. 3):
"A committee from tne National Research Council has concluded that
electromagnetic fields (EMFs) pose no real health threat, as was first
alleged in 1979. The group surveyed more than 500 studies conducted over the
past seventeen years investigating the link between EMFs and, among other
diseases, cancer, reproductive abnormalities, and behavioral problems. They
found that only exposures 1000 to 10,000 times stronger than what is common
in residental settings could alter cellular function; in no study did EMF
exposure affect cellular DNA. (See September 1966, page 80)."
Wil Bigelow, Univ of Michigan !! (bigelow-at-umich.edu)





From: Robert Kayton :      kayton-at-ohsu.edu
Date: Wed, 18 Dec 1996 13:57:32 -0800
Subject: Infrared videomicroscopy

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Hi,

I have been asked to investigate the make up of a system that will be used to do
infrared videomicroscopy, coupled with electrophysiology. I need some idea of
space requirements for the physical setup. I also need to know if there are any
special room requirements ie a darkroom. If you or anyone you know is involved
with this type of activity I would enjoy hearing from you.

Thanks in advance,


Bob Kayton
C.R.O.E.T.
Oregon Health Sciences University
Portland, OR
kayton-at-ohsu.edu




From: Crossman, Harold :      crossman-at-OSI.SYLVANIA.com
Date: Wed, 18 Dec 1996 17:23:11 -0500
Subject: EM fields and health solved!

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My personal theory (not yet published - please don't steal it) is that
hip problems in microscopists are encountered when our hips and
surrounding soft tissues are transferred from uncomfortable chairs in
dark labs to uncomfortable stools in dark barrooms. The greater the
frequency of this transfer, the greater the incidence of hip problems.
Also, the greater the speed at which this seat-seat exchange occurs
(i.e. proximity to the bar from the lab) is significant. I call this
the "Lehigh Factor." There also seems to be a curious, as yet
unresolved, trend toward increasing speed as a function of day of the
week. Monday it is near zero. By Friday, the speed is best described
as "break-neck."

I am entertaining potential grantors of funding for further research
into these orphan phenomena.

Harry Crossman
Osram Sylvania Inc., Beverly, MA
and
The Brewery Exchange, Lowell, MA




From: Tom Phillips :      tphillips-at-biosci.mbp.missouri.edu
Date: Wed, 18 Dec 1996 16:42:26 -0500
Subject: High quality color printer

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I am interested in anyone with experience with high quality color printers.
I already have a Codonics dye sub and am familar with what Tektronix has.
I am thinking of the next step up from this type of printer. Is anyone
familar with the Fuji Pictrography 3000? any other ideas? TIA.


Thomas E. Phillips, Ph.D.
Associate Professor of Biological Sciences
Director, Molecular Cytology Core Facility
3 Tucker Hall
University of Missouri
Columbia, MO 65211
(573)-882-4712 (voice)
(573)-882-0123 (fax)






From: Wil Bigelow :      Wil_Bigelow-at-mse.engin.umich.edu
Date: 18 Dec 1996 17:52:11 -0400
Subject: NRC Rpt on Health & EMFs

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Subject: Time: 5:44 PM
OFFICE MEMO NRC Rpt on Health & EMFs Date: 12/18/96


As a final note on the possible relationship of magnetic fields to
health prob#001##ms, I just noted the following comment in the January
1997 issue of Scientific American (p. 3):
"A committee from the National Research Council has concluded that
electromagnetic fields (EMFs) pose no real health threat, as was first
alleged in 1979. The group surveyed more than 500 studies conducted over the
past seventeen years investigating the link between EMFs and, among other
diseases, cancer, reproductive abnormalities, and behavioral problems. They
found that only exposures 1000 to 10,000 times stronger than what is common
in residental settings could alter cellular function; in no study did EMF
exposure affect cellular DNA. (See September 1966, page 80)."
W. C. Bigelow, Univ of Michigan (bigelow-at-umich.edu)





From: Dave.Strecker-at-po.cle.ab.com (Dave Strecker)
Date: Wed, 18 Dec 1996 18:33:09 -0500
Subject: EDS semi-quant under different conditions?

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I have a question which I have been meaning to pose for some time now.
I have noticed that when I collect a spectrum at 15KeV vs. one at 20
KeV, I get a different (very different) result from a standardless
semi quant on each. The sample in question is a copper coupon used
for corrosion testing with the elements copper, oxygen and sulfur. At
15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and
13%S. Is this normal for a standardless analysis? I have asked the
manufacturer of the EDS but have not received an answer. I would much
appreciate it if someone could clarify this for me.

Thanks in advance.

Dave Strecker
Rockwell Automation/Allen-Bradley Co.

P.S.
Happy Holidays to all, and I hope to see you in Cleveland next August.





From: DDHills :      ddhills-at-mail.idt.net
Date: Wed, 18 Dec 1996 20:30:16 -0800
Subject: SEM Placement

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We are planning to move our SEM into a room of its' own. Are there any
special precautions we should take or things to look out for? I was
wondering specifically about building vibrations. We have noticed a
17Hz vibration on the first floor of our building. Any suggestions will
be welcome as I inherited an old SEM as a "project". TIA.

Deborah Hills-Haney




From: wise-at-vaxa.cis.uwosh.edu
Date: Wed, 18 Dec 1996 20:04:02 +0000
Subject: Re: EM fields and health solved!

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A handful of times per generation, we mere mortals are witness to true
eye-opening, jaw-dropping,
why-didn't-I-think-of-that-?-(slap-on-the-forehead) genius. Harry, I am
humbled in your cyber presence. No wonder my hips hurt so much (especially
on Fridays). And here all these years I thought it was those errant
electrons zipping around the lab.

Bob

Robert R. Wise, PhD
Director, UWO Electron Microscope Facility
Department of Biology
University of Wisconsin Oshkosh
Oshkosh, WI 54901
(414) 424-3404 tel
(414) 424-1101 fax
wise-at-uwosh.edu






From: Heuer Jim P. :      HeuerJ-at-vncpo1.ne.ge.com
Date: Wed, 18 Dec 96 13:18:00 PST
Subject: Re: FE-SEM with WDX

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Joseph -

We have an Amray 1845 FE-SEM with an Oxford (Microspec) WD multi crystal
spectrometer. It works very well for us as we often look at small samples
and want to do high resolution imaging, surface imaging, BSE imaging and
WDS / EDS in the same chamber (although at different working conditions).
We run the Oxford Theta software as to combine ED and WD spectra (and
collect simultaneously). Be aware of some practical issues, however. The
Microspec WDS will be one crystal at a time and therefore slower than a
conventional multi spectrometer microprobe. Also, I recommend you seriously
consider thermally assisted field emission as opposed to cold field
emission, as it will be more stable. Most importantly, remember that to do
WDS AND maintain a small interaction volume, you will need the high
currents, which means a large final aperture, low kV (we see very little
drop in current from 20kV down to 5kV), and a long working distance (that is
where most commercial designs I'm aware of operate). All this means poor
spacial resolution, which may be a show stopper for you. Weigh carefully
all the working parameters and available chamber designs with what you
really want from the instrument you propose. All the best.

No endorsements should be inferred from anything I've said.

James P. Heuer, Ph.D.
General Electric Co.
(510) 862-4501
heuerj-at-vncpo1.ne.ge.com




From: Garber, Charles A. :      cgarber-at-2spi.com
Date: Wed, 18 Dec 96 22:33:54 -0500
Subject: Sputter Targets -

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-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

Jim Harper wrote:
========================================================
} Now the profit motive...I have more than an ounce of gold targets
that
} I just can bear the thought of throwing in the trash can. Anybody
} ever tried to sell the spent targets?
========================================================
The basic problem is this: That one oz. of gold, before it canb e
"converted" back into "money" has to be analyzed by the refiner and the
minimum analysis and refining cost is typically more than the value of gold
in one ounce (or nearly so). So unless you have a dozen sputter coaters
running side by side, all day long, a typical user would be unlikely to ever
generate enough precious metals scrap to make it economic to send back to
the refiner.

That is why we have offered a "trade in" program, send us back your spent
cathodes and we will grant a 10% discount on a new one. When we have
accumulated 20-30 troy ounces of spent cathodes (of a given metal), we then
return them for refining and realize the economic value. This makes a lot
of sense from a non-renewable resource standpoint as well, since once the
world's supply of gold is gone, it is gone.

Note of caution: As with any commodity being set aside for recycling, do
not mix cathodes of different compostions. Keep them separate! They have
greater economic value separated than if they are mixed together and without
traceability as to metal composition.

Chuck

=====================================================
Charles A. Garber, Ph. D. e-mail: cgarber-at-2spi.
com
PRESIDENT
SPI SUPPLIES/STRUCTURE PROBE, INC.
WEST CHESTER, PA 19381-0656 USA

Look us up at
xxxxxxxxxxxxxxxxx
http://www.2spi.com
xxxxxxxxxxxxxxxxx
====================================================




From: Jim Darley :      p&s-at-ultra.net.au
Date: Thu, 19 Dec 1996 14:59:28 +1100
Subject: Re: Texbook SEM / Botany

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} A quick question. We do have Rhodin's Histology Text and Atlas
} which serves us well. Is there anything similar available for SEM
} sample identification?
}
Sverker Enestrom M.D., Ph.D.
****************************************
I note that the questioner is a pathologist. However, for any botanists I
suggest that "An SEM Study of Green Plants" by John N.A. Lott, published by
Mosby in St Louis 1976; ISBN 0-8016-3033-9 is excellent. - If still
available. It's over 20 years now but I still love every picture.
Jim Darley




Probing & Structure (Microscopy Supplies & Accessories)
PO Box 111, Thuringowa QLD 4817 Australia

Phone +61 77 740 370 Fax: +61 77 892 313
A great microscopy site: http://www.ultra.net.au/~pns/





From: Mary Mager :      mager-at-unixg.ubc.ca
Date: Wed, 18 Dec 1996 21:44:29 -0800
Subject: Re: EDS semi-quant under different conditions?

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Dear Dave,
you wrote:
} I have a question which I have been meaning to pose for some time now.
} I have noticed that when I collect a spectrum at 15KeV vs. one at 20
} KeV, I get a different (very different) result from a standardless
} semi quant on each. The sample in question is a copper coupon used
} for corrosion testing with the elements copper, oxygen and sulfur. At
} 15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and
} 13%S. Is this normal for a standardless analysis?
First, are you sure you set up the analysis parameters for the new
conditions? The 15kV electron beam will not fully excite the Cu Ka x-ray
line at 8.4 KeV, so if the Cu peak is lower, the other peaks will analyse as
higher to make up the 100%. You always have to use sufficient over-voltage
(at least double) for the highest energy peak you want to analyse. Of
course, at this much over-voltage the accuracy of the O will be compromised,
but you know you cannot win, only occasionally break even.
Luck,
Mary

Mary Mager
Electron Microscopist
Metals and Materials Eng., UBC
6350 Stores Rd.
Vancouver, B.C. V6T 1Z4
CANADA
tel:604-822-5648, fax:604-822-3619
e-mail: mager-at-unixg.ubc.ca





From: Stephan Coetzee :      stephan-at-gecko.biol.wits.ac.za
Date: Thu, 19 Dec 1996 08:13:02 GMT+2
Subject: Re: Sputter Targets

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Dear All

We get our material from Argen Precious metals in Edenvale
Johannesburg South Africa. (most currency is strong to the rand and may be of a advatage)
They make up to order and may even cut and fit the target material to the target
base (this is by hear say). The advantage is that they keep the waste
and you may get a refund or you may not even have to pay for it!(if it
is true!) They made to spec when ordered. To my knowledge 60% Pd 40% Au is preferable, suppose
to produce a finer coating. . Tell: +27 11 609 8640
Fax: +27 11 452 3918
Standard disclaimer.

Best of luck. And seasons greetings




##
[########]
##
##
##
##
##
Stephan H Coeztee
Electron Microscope Unit
Private Bag 3
Wits
2050
South Africa

Stephan-at-Gecko.biol.WITS.ac.za

Tell: +27 11 716 2419
Fax : +27 11 339 3407




From: Melvyn Dickson :      M.Dickson-at-unsw.edu.au
Date: Thu, 19 Dec 1996 17:27:27 +1100
Subject: Re: Gold Sputter Targets recyclable

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Dear Jim,
Gold is soft and easily worked. In Australia we have many local jewellers
who can refashion gold into usable sputter targets. Try your local yellow
pages. Gold plated onto a baser metal is a different matter.

Mel Dickson





From: Conal Murray :      conal-at-nwu.edu
Date: Thu, 19 Dec 1996 00:43:26 -0500
Subject: SEM Technician Position - OPEN

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============================================================
POSITION OPEN - IMMEDIATELY

SCANNING ELECTRON MICROSCOPE (SEM) TECHNICIAN

An immediate SEM technician position is available at the NU's electron
probe instrumentation center (EPIC). Duties include teaching and
development of laboratories for undergraduate and graduate courses;
training and assistance to users; and techniques and instrumentation
development/modification. Preferred qualifications include a BS degree
in materials related discipline or equivalent and hands-on experience
with SEM, its related techniques and accessories (e.g.evaporators,
sputtering and specimen preparation) - teaching and user training
experience in materials area. Familiarity with modern electronics,
computers, software and hardware, vacuum systems and vacuum
machinery is desired.
Please send resume with salary requirements to:

Human Resources Administration,
Northwestern University
Dept. E96-1029, 720 University Place,
Evanston, IL 60208-1142.

Also send a copy of resume to:

Prof. Vinayak P. Dravid
Materials Science & Engineering
2225 N. Campus Drive, MLSF Building
Northwestern University, Evanston, IL 60208, USA
Ph.: (847) 467-1363, Fax: (847) 491-7820
E-mail: v-dravid-at-nwu.edu

NORTHWESTERN UNIVERSITY is an equal opportunity, affirmative action
educator and employer.
============================================================






From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Wed, 18 Dec 1996 21:26:39 +0000
Subject: Re: FE-SEM with WDX

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} We currently employ an EPMA/SEM system for the analysis of semiconductors
} and packaging materials. Features on these materials are often less than
} one micrometer in size, which necessitates the use of low accelerating
} voltages to minimize the interaction volume. This leads to EDXS peak
} overlap situations, especially for N Ka/TiLa and SiKa/W Ma pairs.
} The use of WDX has been most beneficial for resolving these peak
} overlaps. However, our current LaB6 column lacks the resolution to
} adequately image submicron features and films.
}
} We have considered replacing the LaB6 system with a field emission SEM
} equipped with a WDX spectrometer. Several vendors have demonstrated the
} ability of their FE guns to produce stable beam currents in excess of
} 15nA, which should be sufficient for WDX analyses. I would like to hear
} from any users of FE-SEM/WDX systems as to their experiences and possible
} recommendations. Thanks in advance.
}
} Joseph M. Oparowski
} Digital Equipment Corporation
} 77 Reed Road, HLO2-3/J09
} Hudson, MA 01749-2895
} joseph.oparowski-at-hlo.mts.dec.com

Unless you really need the energy resolution and sensitivity of WDX, isn't
a standard 100kV/EDX TEM system is going to give you all the spacial
resolution you need plus a lot of other opportunities and at a similar cost
to the FE-SEM?

Regards,
Larry Stoter






From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Wed, 18 Dec 1996 21:26:39 +0000
Subject: Re: FE-SEM with WDX

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} We currently employ an EPMA/SEM system for the analysis of semiconductors
} and packaging materials. Features on these materials are often less than
} one micrometer in size, which necessitates the use of low accelerating
} voltages to minimize the interaction volume. This leads to EDXS peak
} overlap situations, especially for N Ka/TiLa and SiKa/W Ma pairs.
} The use of WDX has been most beneficial for resolving these peak
} overlaps. However, our current LaB6 column lacks the resolution to
} adequately image submicron features and films.
}
} We have considered replacing the LaB6 system with a field emission SEM
} equipped with a WDX spectrometer. Several vendors have demonstrated the
} ability of their FE guns to produce stable beam currents in excess of
} 15nA, which should be sufficient for WDX analyses. I would like to hear
} from any users of FE-SEM/WDX systems as to their experiences and possible
} recommendations. Thanks in advance.
}
} Joseph M. Oparowski
} Digital Equipment Corporation
} 77 Reed Road, HLO2-3/J09
} Hudson, MA 01749-2895
} joseph.oparowski-at-hlo.mts.dec.com

Unless you really need the energy resolution and sensitivity of WDX, isn't
a standard 100kV/EDX TEM system is going to give you all the spacial
resolution you need plus a lot of other opportunities and at a similar cost
to the FE-SEM?

Regards,
Larry Stoter






From: Keith Ryan :      KPR-at-WPO.NERC.AC.UK
Date: Thu, 19 Dec 1996 11:40:56 +0000
Subject: EDS semi-quant under different conditions? -Reply

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Dear Dave

I've never been involved with Standardless work, but
around fifteen to ten years ago I did a lot of work with Link's
Quantem and ZAF PB programs. With them you had to store
profiles of elements and quantitative data from your known
standards to derive sensitivity factors using any different
kV's that you wanted to use on specimens, and dial these
(corresponding to the chosen working kV) into an elements
file to apply to unknowns. Maybe this bears on your
problem, in that perhaps you should set-up and work on a
chosen kV?

With best wishes - Keith Ryan

++++++++++++++++++++++++++++++++++++++++++++++++++
Keith Ryan
Plymouth Marine Laboratory, Citadel Hill,
Plymouth, Devon PL1 2PB, England

e-mail: k.ryan-at-pml.ac.uk
PML web site: http://www.npm.ac.uk/pml
++++++++++++++++++++++++++++++++++++++++++++++++++





From: Vladimir Dusevich :      dusevich-at-astro.ocis.temple.edu
Date: Thu, 19 Dec 1996 08:37:19 -0500 (EST)
Subject: Re: EDS semi-quant under different conditions?

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May be you have oxydized surface layer.

On Wed, 18 Dec 1996, Dave Strecker wrote:

} ------------------------------------------------------------------------
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}
} I have a question which I have been meaning to pose for some time now.
} I have noticed that when I collect a spectrum at 15KeV vs. one at 20
} KeV, I get a different (very different) result from a standardless
} semi quant on each. The sample in question is a copper coupon used
} for corrosion testing with the elements copper, oxygen and sulfur. At
} 15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and
} 13%S. Is this normal for a standardless analysis? I have asked the
} manufacturer of the EDS but have not received an answer. I would much
} appreciate it if someone could clarify this for me.
}
} Thanks in advance.
}
} Dave Strecker
} Rockwell Automation/Allen-Bradley Co.
}
} P.S.
} Happy Holidays to all, and I hope to see you in Cleveland next August.
}
}




From: Microscopy-request
Date: Wednesday, December 18, 1996 8:30PM
Subject: SEM Placement

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Deborah:
Most of this you probably thought off already , but here are some of the
things I would do.
I would ask the SEM manufacturer to do a survey of the room for vibrations
and EM fields (they might charge you for this if they are not the ones who
are moving the scope) The position of light fixtures are also important .
Also, I would make sure that there is enough air conditioning capacity to
cool your room and that the air ducts and vents are placed in the right
strategic locations (i.e. as far away from the scope as possible). I would
also check the labs which are adjacent to your new lab. What kind of
equipment do they have ? will it interfere with your SEM ?. What about the
lab upstairs ? We have had a few instances where accidental water leaks
from the labs above us damaged some of our equipment. I hope this helps.

Jordi Marti
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We are planning to move our SEM into a room of its' own. Are there any
special precautions we should take or things to look out for? I was
wondering specifically about building vibrations. We have noticed a
17Hz vibration on the first floor of our building. Any suggestions will
be welcome as I inherited an old SEM as a "project". TIA.

Deborah Hills-Haney




From: Eric Steel :      eric.steel-at-nist.gov
Date: Thu, 19 Dec 1996 11:05:48 -0500
Subject: Re: EDS semi-quant under different conditions?

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}
} I have a question which I have been meaning to pose for some time now.
} I have noticed that when I collect a spectrum at 15KeV vs. one at 20
} KeV, I get a different (very different) result from a standardless
} semi quant on each. The sample in question is a copper coupon used
} for corrosion testing with the elements copper, oxygen and sulfur. At
} 15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and
} 13%S. Is this normal for a standardless analysis? I have asked the
} manufacturer of the EDS but have not received an answer. I would much
} appreciate it if someone could clarify this for me.
}
} Thanks in advance.
}
} Dave Strecker

This difference in analysis result may be real or artifact. It may be due
to the larger (deeper) excitation volume at 20 kV, so that more Cu is
excited relative to the surface O and S. Or it could be an artifact due to
the standardless approach. I would suggest using a standards analysis and
trying again. This should eliminate part of the problems (such as the
overvoltage dependence suggested by Mary Mager)

Varying the voltage has long been used to analyze thin films relative to the
substrate (as might be expected in certain corrosion systems). There is a
freeware routine called "GMRFilm" on the Microscopy and Microanalysis
software reference library at http://www.msa.microscopy.com/RefEdu.html.
(the GMRFilm software can be found under
ftp://ftp.msa.microscopy.com/pub/4-MMSLib/XEDS/) that allows one to analyze
films using this method. There is also at least one commercial package
which will fit the multivoltage data to determine the best composition of
one or more films on a substrate. Both these packages will require the
comparison of the unknown to standard bulk/polished materials. Monte Carlo
modelling can also be used to understand the thin film/substrate
compositions. There are several public domain monte carlo programs
available also.


Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov
Microanalysis Research Group Office: 301-975-3902
N.I.S.T. FAX: 301-216-1134
Bldg. 222/Rm A113
Gaithersburg MD 20899





From: Gib Ahlstrand :      giba-at-puccini.crl.umn.edu
Date: Thu, 19 Dec 1996 10:27:05 -0600
Subject: Re: EDS semi-quant under different conditions?

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In message {0000FA68.1893-at-po.cle.ab.com} Dave Strecker writes:

} I have a question which I have been meaning to pose for some time now.
} I have noticed that when I collect a spectrum at 15KeV vs. one at 20
} KeV, I get a different (very different) result from a standardless
} semi quant on each. The sample in question is a copper coupon used
} for corrosion testing with the elements copper, oxygen and sulfur. At
} 15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and
} 13%S. Is this normal for a standardless analysis? I have asked the
} manufacturer of the EDS but have not received an answer. I would much
} appreciate it if someone could clarify this for me.
}
} Thanks in advance.
}
} Dave Strecker
} Rockwell Automation/Allen-Bradley Co.

Dave,

Send me your FAX number and I will send you a two page reprint from the
Microscopoy & Microanalysis '96 meeting held this past August in Minneapolis.
I'll send you Dale Newbury's article entitled: "Standardless" Quantitative
Electron Probe X-ray Microanalysis with Energy-Dispersive Spectrometry: What is
the Distribution of Errors?

Dale (from NIST, Gaithersburg, MD) is one of the Microbeam Analysis Society's
traveling speakers this year and for that his talk is entitled: Lies, Damned
Lies, and "Standardless Analysis". I think from the title alone you get the gist
of it. I've heard the talk and its quite revealing. The reprint I'll send you is
a good introduction to the criticism of standardless analysis. Dale's conclusion
is basically (in my own words): The Truth, the Whole Truth, and Standards-Based
Analysis.




Gib Ahlstrand, MMS Newsletter Editor
Electron Optical Facility, University of Minnesota, Dept. Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu

"When the mode of the music changes, the walls of the city will shake." - PLATO
"There's a whole lotta shakin' goin' on!" - CHUCK BERRY





From: Neal Nicklaus :      nnicklaus-at-nova.sarnoff.com
Date: Thu, 19 Dec 1996 11:43:57 -0500
Subject: Re: Infrared videomicroscopy

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What wavelength region are you interested in?

There are some commercial sources for Near IR (0.7 to ~1 micron) video
microscopy. I think I have also seen some commercial sources related to the
mid-wave IR (3-5 microns) and/or long-wave IR (8-12 microns).

I know something about the camera systems available in the IR regions, if
that would help.



At 01:57 PM 12/18/96 -0800, you wrote:
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Neal Nicklaus
Senior Scientist
SEQ Limited

Voice: 609-452-6033 Ext. 13
Fax: 609-452-5955
email nnicklaus-at-seq.sarnoff.com





From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 19 Dec 1996 11:56:32 -0500 (EST)
Subject: Re: NRC on EMFs & Health

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} Subject: Time: 4:15 PM
} OFFICE MEMO NRC on EMFs & Health Date: 12/18/96
}
} As a final note on the possible relationship of magnetic fields to health
} problems, I just noted the following comment in the January 1997 issue of
} Scientific American (p. 3):
} "A committee from tne National Research Council has concluded that
} electromagnetic fields (EMFs) pose no real health threat, as was first
} alleged in 1979. The group surveyed more than 500 studies conducted over the
} past seventeen years investigating the link between EMFs and, among other
} diseases, cancer, reproductive abnormalities, and behavioral problems. They
} found that only exposures 1000 to 10,000 times stronger than what is common
} in residental settings could alter cellular function; in no study did EMF
} exposure affect cellular DNA. (See September 1966, page 80)."
}
Dear Wil,
The bionet.emf-bio newsgroup had several comments about this report.
Among them was the statement that the report actually said that there was
no evidence for a health threat (Absence of evidence is not evidence of
absence. :-) ). From what I've heard, there is some evidence that 60 Hz
fields, especially, e.g., from electric blankets, has been correlated with
statistical increases in childhood cancer. This evidence is tenuous, and
not all experts are in agreement. The important features are that the field
is produced near (~1 cm) the exposed person, and the person has a very long
(~8 hr) exposure.
Kershvinck (sp?) at Caltech has investigated the interaction of low-
level EMF's with magnetite crystals associated with cell membranes, and he
found that 10's of Hz is the order of magnitude for resonant frequencies.
The energy calculations show that it is possible for such a crystal to
affect a transport protein. The conclusion is that there is a physically
possible mechanism for a low-level AC field to cause biological effects.
For me the bottom line is that I would not use an electric blanket
or other appliance that I'd be close to for long periods, but I'd be much
more concerned over many other environmental hazards (air, H2O, food qual-
ity, etc.).
Yours,
Bill Tivol




From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 19 Dec 1996 12:06:40 -0500 (EST)
Subject: Re: FE-SEM with WDX

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} Unless you really need the energy resolution and sensitivity of WDX, isn't
} a standard 100kV/EDX TEM system is going to give you all the spacial
} resolution you need plus a lot of other opportunities and at a similar cost
} to the FE-SEM?
}
Dear Larry,
It all depends on what info is required. A TEM+EDX system uses a
thin specimen which is penetrated by the beam, so the emission volume is
determined by the incident beam size and the spread through the specimen.
For our HVEM, the worst case, our incident beam is ~.4 micron, and the
spread through a 1 micron section is about the same. This works well for
~1 micron resolution in x, y, and z, which is OK for biological sections.
For 100 kV, the beam size is typically smaller, and the spread through a
.1 micron section is also { { 1 micron. However, the emission volume is
the entire thickness of the section. If surface measurements are needed,
the SEM is a better choice.
Yours,
Bill Tivol




From: William Tivol :      tivol-at-wadsworth.org
Date: Thu, 19 Dec 1996 12:16:29 -0500 (EST)
Subject: Re: SEM Placement

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} We are planning to move our SEM into a room of its' own. Are there any
} special precautions we should take or things to look out for? I was
} wondering specifically about building vibrations. We have noticed a
} 17Hz vibration on the first floor of our building. Any suggestions will
} be welcome as I inherited an old SEM as a "project". TIA.
}
Dear Deborah,
Either of two possible mounting strategies can be chosen depending
on the vibration environment. The column can be isolated from the building
or it can be firmly anchored. If the building vibrations are large, the
instrument has to be isolated, but if acoustic vibrations } } building vibs.
then isolation may actually degrade performance. I have seen a TEM which
could have been used as "The Clapper (TM)". Good luck.
Yours,
Bill Tivol





From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Wed, 18 Dec 1996 21:26:34 +0000
Subject: Re: Electromagnetic Fields & Health

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It seems to me that a little perspective is called for in this, and any
other debate on health risks.

If governments, consumer groups, etc applied themselves a little more to
risks like tobacco, cars (direct and pollution), alcohol and guns then
worrying about EMF might be might be relevant.

Regards,
Larry Stoter






From: Woody.N.White%650 :      Woody.N.White-at-mcdermott.com
Date: 12/18/96 6:28 PM
Subject: EDS semi-quant under different conditions?

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Another posibility.... Is the the film analyzed thicker than the depth
of you analysis volume? If the analysis volume extends into the Cu
substrate more at 20 kV than at 15 kV (or less), then your results are
understandable. _Woody_

Woody White
http://www.geocities.com/capecanaveral/3722
___________________________ Reply Separator
_________________________________


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I have a question which I have been meaning to pose for some time now.
I have noticed that when I collect a spectrum at 15KeV vs. one at 20
KeV, I get a different (very different) result from a standardless
semi quant on each. The sample in question is a copper coupon used
for corrosion testing with the elements copper, oxygen and sulfur. At
15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and
13%S. Is this normal for a standardless analysis? I have asked the
manufacturer of the EDS but have not received an answer. I would much
appreciate it if someone could clarify this for me.

Thanks in advance.

Dave Strecker
Rockwell Automation/Allen-Bradley Co.

P.S.
Happy Holidays to all, and I hope to see you in Cleveland next August.




From: Dr. H. Adelmann :      106421.3362-at-CompuServe.COM
Date: Thu, 19 Dec 1996 14:18:39 -0500
Subject: taricha lung cells wanted

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Hello all,

I looking for a cell culture laboratory -preferable in Germany- but may be
anywhere, that could supply me with "stock cell culture" material of
Taricha granulosa (newt) lung cells. I have to generate some time lapse
videos of its cell division in DIC/phase contrast-microscopy.

Thanks

Holger G. Adelmann
{106421.3362-at-compuserve.com}




From: Nancy McInerney :      NMCINERNEY-at-ACDM.RDC.AB.CA
Date: Thu, 19 Dec 1996 12:26:37 -0600 (MDT)
Subject: Leica/Hammond transformers

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Our college has purchased hundreds of microscopes and dissecting scopes
over the years. The bulk of them are Wild/Leitz/Leica brand. Some have external
transformers while others are built in. Last year we purchased 10 dissecting
scopes with external transformers from Leica. The transformers were manufactured
by Hammond Manufacturing (we have made similiar component purchases in the
past). We have had all of these transformers fail, have returned them, had them
replaced and the replacements have failed. Our maintenance dept looked at a few
and noticed that the wire on the transformers is much thinner than the wire on
the older models. We have never experienced this problem before. Leica is now
refusing to replace these transformers and I have 10 non-functional
transformers. If anyone has any suggestions I would appreciate hearing them.
You can email me directly at nmcinerney-at-rdc.ab.ca.

I appreciate any advice anyone has to offer.

Nancy McInerney
Red Deer College
Red Deer Alberta
403-342-3142





From: Warren Straszheim :      wes-at-ameslab.gov
Date: Thu, 19 Dec 1996 13:58:36 -0600
Subject: Re: EDS semi-quant under different conditions?

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At 06:33 PM 12/18/96 -0500, you wrote:
} I have a question which I have been meaning to pose for some time now.
} I have noticed that when I collect a spectrum at 15KeV vs. one at 20
} KeV, I get a different (very different) result from a standardless
} semi quant on each. The sample in question is a copper coupon used
} for corrosion testing with the elements copper, oxygen and sulfur. At
} 15 KeV I get 46%Cu, 34%O and 18%S. At 20KeV I get 63%Cu, 23%O and
} 13%S. Is this normal for a standardless analysis? I have asked the
} manufacturer of the EDS but have not received an answer. I would much
} appreciate it if someone could clarify this for me.
}
} Thanks in advance.
}
} Dave Strecker
} Rockwell Automation/Allen-Bradley Co.

Dave,
Would you provide a few more specifics? It might help to know the
manufacturer and software. If you tell us, I/we will try not to be too
disparaging.

I presume you told the quant setup that you were changing kV. If you didn't,
that would explain a lot.

We decided against a Kevex system back in the early 80's because its
background treatment did not seem as good as Tracor Northern's at the time.
But we bought a Kevex system in the late 80's after we were shown some more
of its features. We were shown an absorption factor that enters into
background modeling that can have a significant effect on the results. I
suppose it factors into the correction the sensitivity for lines at
different energies (since you do not have elemental standards). If a top-hat
filter is used to remove the background, then the quant would/could not be
tailored to fit your particular system's sensitivity, unless your system had
somehow already been calibrated for that effect.

Having said all that, I will join with the others who will undoubtedly chime
in and ask what do you expect for standardless analyses anyway? I often use
them, but I have to remember they are only as good as the effort I invest in
them.

----------------------------------------------------
Warren E. Straszheim
270 Metals Development, Ames Lab/ISU, Ames IA, 50011
Phone: 515-294-8187 FAX: 515-294-3091

E-Mail: wes-at-ameslab.gov (or: wesaia-at-iastate.edu)
http://www.public.iastate.edu/~iprt_info/cfce/ (re: coal)
http://www.public.iastate.edu/~wesaia/marl/ (re: SEM)

coal characterization and processing
electron microscopy, x-ray analysis, image analysis
computer applications





From: Kathy Walters :      kwalters-at-emiris.iaf.uiowa.edu
Date: Thu, 19 Dec 1996 14:02:30 -0600 (CST)
Subject: Re: Texbook SEM Biological

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microscopy-at-Sparc5.Microscopy.Com

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I was under the impression that Kessel's book was out of print??!!

Kathy Walters / /
Research Assistant III / /\
Center for Microscopy Research / /\ \
University of Iowa /_/ \ \
85 EMRB ____ ((O))
Iowa City, Iowa 52242 | | / /
|| / /
email: kwalters-at-emiris.iaf.uiowa.edu -----------
fax: (319)335-8049 -------------
www: http://www.uiowa.edu/~cemrf


On Wed, 18 Dec 1996, Sverker Enestrom wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
}
} } A quick question. We do have Rhodin's Histology Text and Atlas
} } which serves us well. Is there anything similar available for SEM
} } sample identification?
} }
} Greetings,
} Johannes Rohdin's Histology is from 1974 and a SEM atlas I know
} is from 1979: Kessel RG and Kardon RH: Tissues and Organs, a text-atlas
} of scanning electron microscopy. W.H. Freeman and Company, San Francisco
} ISBN 0-7167-0091-3
} Best regards.
} Sverker
}
} *********************************************************
} Sverker Enestrom M.D., Ph.D.
} Department of Pathology
} University of Linkoping, Sweden
} Phone: +46 13 22 15 20
} Fax: +46 13 13 22 57
} *********************************************************
}
}





From: Sara Miller :      saram-at-acpub.duke.edu
Date: Thu, 19 Dec 1996 18:25:48 -0500 (EST)
Subject: Re: SEM Placement

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On Wed, 18 Dec 1996,
DDHills wrote:

} Date: Wed, 18 Dec 1996 20:30:16 -0800
} From: DDHills {ddhills-at-mail.idt.net}
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: SEM Placement
}
} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
}
} We are planning to move our SEM into a room of its' own. Are there any
} special precautions we should take or things to look out for? I was
} wondering specifically about building vibrations. We have noticed a
} 17Hz vibration on the first floor of our building. Any suggestions will
} be welcome as I inherited an old SEM as a "project". TIA.
}
} Deborah Hills-Haney
}
Dr. Judy Murphy is an expert in this field (jmurphy-at-sjdccd.cc.ca.us).
Maybe you should have her come in and look at your site.
S.

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735





From: John Best :      jbest-at-vicon.net
Date: Thu, 19 Dec 1996 22:28:50 -0800
Subject: Duplicate messages

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Hello Nestor,

Would you have any explanation as to why I'm getting duplicates of all
the email I receive from the MSA listserver? Thank you for any ideas
you have.

Regards,
John.

--
ELMDAS Co. P.O. Box 355, Alexandria, PA 16611
Voice: (814) 669-4474
Our website: http://www.vicon.net/~jbest
Email: jbest-at-vicon.net





From: Eric Steel :      eric.steel-at-nist.gov
Date: Fri, 20 Dec 1996 08:48:51 -0500
Subject: Re: EDS semi-quant under different conditions?

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}
} Send me your FAX number and I will send you a two page reprint from the
} Microscopoy & Microanalysis '96 meeting held this past August in Minneapolis.
} I'll send you Dale Newbury's article entitled: "Standardless" Quantitative
} Electron Probe X-ray Microanalysis with Energy-Dispersive Spectrometry:
What is the Distribution of Errors?
}
} Dale (from NIST, Gaithersburg, MD) is one of the Microbeam Analysis
} Society's traveling speakers this year and for that his talk is entitled:
} Lies, Damned Lies, and "Standardless Analysis". I think from the title
alone } you get the gist of it. I've heard the talk and its quite revealing.
The } reprint I'll send you is a good introduction to the criticism of
standardless } analysis. Dale's conclusion is basically (in my own words):
The Truth, the } Whole Truth, and Standards-Based Analysis.
}
} Gib Ahlstrand, MMS Newsletter Editor

Another good reference on the same subject:

Newbury, D. E., Swyt, C. R., and Myklebust, R. L., "'Standardless'
Quantitative Electron Probe Microanalysis with Energy-Dispersive X-ray
Spectrometry: Is It Worth the Risk?", Analytical Chemistry, 67 (1995)
1866-1871.



Eric B. Steel, Leader e-mail: eric.steel-at-nist.gov
Microanalysis Research Group Office: 301-975-3902
N.I.S.T. FAX: 301-216-1134
Bldg. 222/Rm A113
Gaithersburg MD 20899





From: Kirill E. Prikhodko :      KIRILL-at-nw.oirtorm.net.kiae.su
Date: Fri, 20 Dec 1996 13:57:00 +300 (MSK)
Subject: RCPT: TEM powder prep - THANKS

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Confirmation of reading: your message -

Date: 12 Dec 96 17:29
To: microscopy-at-Sparc5.Microscopy.Com
Subject: TEM powder prep - THANKS

Was read at 13:56, 20 Dec 96.





From: Kirill E. Prikhodko :      KIRILL-at-nw.oirtorm.net.kiae.su
Date: Fri, 20 Dec 1996 14:51:10 +300 (MSK)
Subject: TEM: Si chemical etching

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Dear colleagues,

We have to prepare Si TEM specimens by chemical etching technique.
All our attempts failed due to the micro- or macro- pitting. We
used a various combinations of HF, HNO3 and CH3COOH but without any
success. Does anyone know the good content of the etching solution
and regimes of chemical etching?

Thanks in advance.
Happy Christmas and New Year.

Kirill Prikhodko
Russian Research Center "Kurchatov Institute"
Moscow
E-mail: kirill-at-nw.oirtorm.net.kiae.su




From: Larry Stoter :      LPS-at-teknesis.demon.co.uk
Date: Wed, 18 Dec 1996 21:26:44 +0000
Subject: Re: Sputter Targets - Thanks

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} Now the profit motive...I have more than an ounce of gold targets that
} I just can bear the thought of throwing in the trash can. Anybody
} ever tried to sell the spent targets?
}
} Jim Harper
} jeharper-at-amoco.com

I suspect the answer is the same as for all those people whoe save their Pt
apertures or want to recycle the silver in their dark rooms - unless you
are in a situation where you can operate on an appropriately large scale,
the time and effort simply doesn't justify the return.

Regards,
Larry Stoter






From: Donna Turner :      dturner-at-bcm.tmc.edu
Date: Fri, 20 Dec 1996 09:48:19 -0600
Subject: Liposomes

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I need information regarding processing for thin-sectioning of
liposomes. These samples are used for Cyclosporin A treatment (inhalation)
of lung tumors. I will also be using negative staining. Suggestions please!





From: clsmteam-at-imiucca.csi.unimi.it
Date: Fri, 20 Dec 1996 08:32:06 GMT
Subject: Unscribe

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Please unscribe Cristiano Rumio


Dr. Cristiano Rumio
Istitute of Anatomy
University of Milan
Via Mangiagalli 31, 20133 Milan
Italy
E-mail clsmteam-at-imiucca.csi.unimi.it
Voice: -39.2.2663683
Fax:-39.2.2364082





From: Ronald M. Anderson (1-914-892-2225) :      ron-anderson-at-vnet.ibm.com
Date: Fri, 20 Dec 96 09:45:47 EST
Subject: TEM: Si Chemical Etch

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Message-Id: {199612201536.JAA00753-at-Sparc5.Microscopy.Com}

We've been backside etching Si with HF-Nitric-Acetic (H:N:A) since the
1950s. Formulas have changed over the years. Currently we use
H:N:A = 6:10:5. Various ratios of the components produce
etches that are faster or slower. Myrtle Ellington wrote a fine paper
on this in MRS Symposium Proceedings 115, p. 265, where she had different
etch rates with different formulations used in the beginning, middle, and
at the end of the etching procedure. Her ratios H:N:A = 4:1:2 initally,
10:1:0 and then 15:1:0 for the final etch. Obviously there is a wide
range of formulas that etch Si.

With our 6:10:5 formula we start etching with fresh etchant and see
rapid thinning with a lot of bubble evolution. For the final, smooth,
bubble-free etching step we use old solution in the same ratio that
is a couple of weeks old. We mask the Si in wax* on a glass microscope
slide and apply one drop of etchant at a time. In the early, bubbly,
stage we remove the etch drop with a Q-tip (cotton bud) after a few
seconds.** In later stages the old solution can be left on the Si
for up to 30 seconds.***

We tripod polish the plan view Si from the back until the Si is 200-300um
thick before etching. With Si this thin, etching is finished in a couple
of minutes.****

* Use real beeswax, from bees, dissolve in xylene. If a lot of ion
milling is anticipated use a cyanoacrylate (super glue) instead of wax.
** Alternatively, especially in later stages, remove the old etchant
by dipping the entire slide in distilled water and blow dry. Allows for
safe microscope examination.
*** At the very, very end, while you are monitoring the extent of the
thin area, leave the etchant on for very short times between examinations.
Let it go for 30 seconds only when it is safe to do so.
****Thin starting Si allows for low angle ion milling the hole side.
Would get the same result with large wheel dimpling a'la Helen Humiston.


Ron Anderson




From: Barry, Lilith :      Lilith.Barry-at-nrc.ca
Date: Fri, 20 Dec 1996 11:29:00 -0500
Subject: Non radioactive in-situ hybridization

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There seem to different ways to label oligonucleotides without using
radioactive probes. There are few companies that carry products for it.
Could someone please tell me which is their preferred method and which
company they deal with.
Thanking you in advance,

Lilith Ohannessian-Barry
NRC, IBS,
Ottawa, Ont. K1A 0R6
Tel;613-993-6460
Fax;613-941-4475
e-mail; lilith.barry-at-nrc.ca




From: South Bay Technology :      73531.1344-at-CompuServe.COM
Date: 20 Dec 96 14:56:29 EST
Subject: Re: TEM: Si chemical etching

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Dear Dr. Prikhodko:

A technique was developed many years ago by one of our customers for chemcially
thinning silicon for TEM. The process does involve the use of our Model 550D
Single Vertical Jet Electropolisher which has been set up in chemical etching
mode.

The process involves using a polishing solution of 360ml nitric acid and 90ml
HF. The jet nozzle is placed 3 to 4mm above the sample and the flow rate set as
slow as possible without breaking the solution into drops above the sample. In
electrolytic mode, we would typically use an infrared LED in our detector
circuit, but since silicon is transparent to infrared, we replace the infrared
with a green LED to allow us to automatically terminate the process.

Some of this may not make sense as most people are familiar with the twin-jet
configuration. The Model 550D does not immerse the sample in the solution - it
sits on a pedestal (sounds appropriate for a very important sample!) and the
solution is flowed onto the sample from the top side only. While it may sound
inefficient to polish from just one side, there are actually a significant
number of advantages which has allowed us to prepare samples that were
previously impossible with a twin jet system. I'd be pleased to discuss these
points off-line with anyone who has an interest.

If you would like to receive a copy of the procedure for thinning silicon,
please contact me and I can FAX and/or mail a copy of the procedure to you. I
also have a bibliography of technical reports dealing with TEM sample
preparation. I would be pleased to send this to anyone who has an interest.
You can then request reprints of any of the papers and we will send them out to
you at no charge.

I hope this helps!

Best regards-

David Henriks TEL: 800-728-2233 (toll-free in USA)
South Bay Technology, Inc. 714-492-2600
1120 Via Callejon FAX: 714-492-1499
San Clemente, CA 92673 USA e-mail: henriks-at-southbaytech.com

************** PLEASE VISIT OUR WEB SITE! **************

http://www.southbaytech.com

Manufacturers of Precision Sample Preparation Equipment and Supplies for
Metallography, Crystallography and Electron Microscopy.


YOUR MESSAGE:
Dear colleagues,

We have to prepare Si TEM specimens by chemical etching technique.
All our attempts failed due to the micro- or macro- pitting. We
used a various combinations of HF, HNO3 and CH3COOH but without any
success. Does anyone know the good content of the etching solution
and regimes of chemical etching?

Thanks in advance.
Happy Christmas and New Year.

Kirill Prikhodko
Russian Research Center "Kurchatov Institute"
Moscow





From: Tan-Chen Lee :      Tan-Chen_Lee-at-mesaqm.sps.mot.com
Date: 20 Dec 1996 12:52:57 U
Subject: Re: TEM- Si chemical etching

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X-Mailer: Mail*Link SMTP-QM 4.0.0



From: Donald Lovett :      lovett-at-tcnj.edu
Date: Fri, 20 Dec 1996 14:18:57 -0500 (EST)
Subject: Wanted to Buy: Used EDAX/EDS detector for TEM

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Reply to: RE} TEM: Si chemical etching

Kirill:
It is not the ratio of acids which caused problem. I used to do chemical
etching in Taiwan. It went fine. However, the same recipe resulted in
pitting on Si(100) but not on Si(111) when I tried it in New York. It seemed
that preferential etching happened. Even when I did it in clean room or
change recipes, it did not solve the problem. I suspect the possible reasons
are the contents of the acids (concentration, contaminants, etc.),
temperature, sample holders (Teflon versus glasses??), wax, etc. You may only
need to change the vendors of the chemicals. I would also like to know the
real cause though I do not do chemical ethcing any more.

Tan-Chen Lee
Materials Characterization Lab
Motorola, Inc.
--------------------------------------

Dear colleagues,

We have to prepare Si TEM specimens by chemical etching technique.
All our attempts failed due to the micro- or macro- pitting. We
used a various combinations of HF, HNO3 and CH3COOH but without any
success. Does anyone know the good content of the etching solution
and regimes of chemical etching?

Thanks in advance.
Happy Christmas and New Year.

Kirill Prikhodko
Russian Research Center "Kurchatov Institute"
Moscow
E-mail: kirill-at-nw.oirtorm.net.kiae.su

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I have an Hitachi H-7000 with SEM/STEM mode. I am in need of an x-ray
detector and associated hardware to do elemental analysis (EDAX or EDS or
whatever the proper generic term is). As a small 4-year college, we would
much rather go the route of purchasing a used system.

Please send any replies directly to me. Thank you.


______________________________________________________________________
Donald L. Lovett e-mail: lovett-at-tcnj.edu
Assoc. Professor, Dept. of Biology voice: (609) 771-2876
The College of New Jersey * fax: (609) 771-2674
Trenton, NJ 08650-4700

(* formerly Trenton State College; please note our new name)






From: BobCat54-at-aol.com
Date: Fri, 20 Dec 1996 19:43:29 -0500
Subject: Carl Zeiss light microscope - Help

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I would very much appreciate some information on a Carl
Zeiss light microscope. This is an older model binocular unit
with a five objective nose piece. The two eye pieces are marked
8x KPL. What is KPL? The five objectives lenses are 10x/0.22,
16x/0.40, 25x/0.60, 40x/0.65 and 100x/1.30-NEOFLUAR (what is
NEOFLUAR?). I don't know if the 100x is oil or not. There is a
number on the top that looks like 4254092. Near the eye piece is
4656721. On the base is 4291647. Near the filter holder is
4653272. There are two filter holders, one with a clear glass
and one empty. The condenser has a rack and pinion for movement
and a swing out lens marked ".9". The built-in light source has
an external AC power transformer/controller with "TYP 392524" and
"Regel Transformator" marked on it.
So, with all that, can you tell me the age and worth of this
microscope. Also, the availability of replacement parts. Will
this unit take standard eye pieces and objective lenses etc.?
Any other information you can offer will be most helpful. Thank
you in advance for your time and help.

R.H. Isabelle
4 Pioneer Way
Springfield, MA 01119-1720
E-mail: bobcat54-at-aol.com





From: Dr. H. Adelmann :      106421.3362-at-CompuServe.COM
Date: Sat, 21 Dec 1996 18:44:02 -0500
Subject: rotocompressor

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Hello all,

I am looking for a device called "Rotocompressor". It is a kind of chamber
to put onto the microscopic stage to observe protists or other cells, while
being able to rotate them into position and/or applying some pressure to
them in order to flatten them to observe specific detail. I think it was
first used by American protozoologists.

Any help would be greatly appreciated.

Holger G. Adelmann

{106421.3362-at-compuserve.com}




From: A. Greene :      ablue-at-mail.io.com
Date: Sat, 21 Dec 1996 17:33:29 -0600 (CST)
Subject: Re: TEM phosphor plates vs. CCD camera systems

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Hi Lucille, I guess you know the SIMS is ready to go. The job was
uneventful except for the fact that I slipped on the ice and fell down the
motel stairs. I guess a little extra padding is good for something. While
on the subject of physical things, I hope your cold is better. You really
sounded awful.

I wanted to get there between the SIMS and Christmas but I have had three
service calls since my return. I really have to hire somebody to help me.
Clearly, the business is growing and I can't stretch much farther.

Please recall that you were going to send a drawing of the rooms which I am
to survey. A reduced (small) general diagram would do just fine. I guess
you received my revised quote. I hope it is acceptable.

Well...have a great Christmas and a Happy, Healthy New Year.

Alex Greene
Scientific Instrumentation Services, Inc.
Austin, Texas

At 07:39 AM 12/11/96 -0500, you wrote:
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From: Paul.Fischione-at-internetmci.com
Date: Sun, 22 Dec 1996 19:55:45 -0500
Subject: Re: TEM phosphor plates vs. CCD camera systems

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-- [ From: Paul E. Fischione * EMC.Ver #2.3 ] --

With regard to Kirill Prikhodko's request, a recommended procedure for
preparing Si is by chemical jet etching. The solution used is typically HF
based. There are many which are quite sufficient. The following are a few
which have been proven satisfactory:

1.) 90% Nitric acid 10% HF
2.) 5 parts Nitric acid, 3 parts Acetic acid, 3 parts HF. Polish at room
temperature.
3.) 10.5 grams Potassium Permanganate, 300ml HF, 30ml De-ionized Water.
Polish at -20 to -30 degrees C with a high jetting speed.

Depending on the orientation of the Si, the percentage of the acids may
need to be altered.

These solutions and conditions have provided excellent results when
utilizing the twin-jet electropolishing technique which simultaneously
thins both specimen surfaces. If it is desired to back-thin the Si, one
side should be protected with Beeswax.

For the electrolytic polishing of metals it is recommended to have both the
specimen and the jets submerged in the electrolyte by approximately 3-4mm.
When chemical etching of Si, it is recommended that the specimen be above
the chemical solution level. The jet position in relation to the specimen
can be varied to provide the necessary configuration of the dimple produced
by the chemical etching process.

Kind regards for a Happy Holiday Season,

Paul Fischione

E.A. Fischione Instrument, Inc. is the manufacturer of the Automatic Twin-
Jet Electropolisher.






From: ROBIN CROSS :      EURC-at-giraffe.ru.ac.za
Date: Mon, 23 Dec 1996 08:12:36 GMT+0200
Subject: RCPT: TEM powder prep - THANKS

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Confirmation of reading: your message -

Date: 12 Dec 96 17:29
To: microscopy-at-Sparc5.Microscopy.Com
Subject: TEM powder prep - THANKS

Was read at 8:12, 23 Dec 96.


Robin H Cross
Director : EM Unit, Rhodes University, Grahamstown, South Africa
eurc-at-giraffe.ru.ac.za - tel: +27 461 318169 - fax: +27 461 24377




From: Michel Deschuyteneer :      deschuyt-at-sbbio.be
Date: Mon, 23 Dec 96 09:40:04 +0100
Subject: Re: Liposomes

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Donna:

The best technique to analyse liposomes is cryo-TEM, by the direct
observation of a thin frozen (actually vitrified) hydrated suspension.
Alternatively, you may use the freeze fracture replicas approach.
Good luck.

Regards,
Michel

****************************************************
Michel Deschuyteneer, Ph.D. deschuyt-at-sbbio.be
Scientist Electron Microscopy Laboratory

SmithKline Beecham Biologicals
Rue de l'Institut, 89 B1330 Rixensart, BELGIUM
Tel: +32-2-656 9290 Fax: +32-2-656 8164
****************************************************
Standard disclaimer: the opinions expressed in this
communication are my own and do not necessarily
reflect those of SmithKline Beecham.
****************************************************





From: Lynnette Madsen :      lyn-at-ifm.liu.se
Date: Mon, 23 Dec 96 09:53:16 +0200
Subject: listserver

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In recent weeks, I have had so many inquires, I thought I should send a general
e-mail.

Below is
i. reminder notice regarding 2nd Northern Workshop on
TEM Sample Preparation
of Thin Films - ceramics, metals, semiconductors-

ii. 1st flyer for same.

___________________________
reminder

2nd Northern Workshop on
TEM Sample Preparation
of Thin Films - ceramics, metals, semiconductors-

Link|ping University, Link|ping, Sweden

February 25-28, 1997


Deadline for registration and
abstracts:
January 10, 1997; limited entries may be accepted up to February 20, 1997

Our original mailing list included Sweden, Denmark, Norway, Finland, Canada, USA,
Belgium, France, Germany, The Netherlands, Hungary, Switzerland, Ireland, England,
Japan and Australia. Additional inquiries have led to (further) distribution in the
Ukraine, UK, USA, France, India, Belgium, Spain, Russia, Denmark and Sweden.
Additionally, we have advertised at the following conferences: EUREM, AVS, SCANDEM,
as well as by several electronics means.
-} Number of registrants will be limited.

Second and Final announcement with the Preliminary program
will follow after the above deadline in January 1997

Advance payment can be made via Swedish Post Giro to
account 183415-9 Link|ping University.
Mark the pay-slip with the project number 12276001 !!

On-site payment can be made in Swedish or USA cash only.
___________________________
Call for Papers & Registration, revised 1st Announcement
2nd Northern Workshop on
TEM Sample Preparation
of Thin Films - ceramics, metals, semiconductors-

Link|ping University, Link|ping, Sweden

February 25-28, 1997.


Basic and Advanced Sample Preparation Techniques
Oral and poster presentations

Invited Speakers
Gy|rgy Radnoczi, Res. Inst. for Tech. Phys. of Hungarian Academy of Sciences,
Low Angle Ion Beam Technique; Practice and Theory for Cross-sectional Samples

Tom Malis, Materials Technology Laboratory, Natural Resources Canada,
Microtomy for the Material Sciences

Ron Anderson, IBM East Fishkill, NY USA,
Tripod Polishing and FIB Techniques for Precision TEM specimen preparation

Michael Phaneuf, Chipworks Inc., Ottawa Canada & Link|ping University,
TEM Replication Techniques Applied to Semiconductor Dopant Profiling;
Dental Composites for Low-Temperature XTEM of Galvannealed Steels

Equipment Exhibit (including all types of microscopy tools and instruments)

Demonstrations and Hands-on Laboratory Sessions
(will occupy 50% of scheduled workshop time)

Exhibitors / Sponsors
NorFA Network on "Materials Studies with Electron Microscopy Studies"
Nanometric Systems & South Bay Technology, Inc.,
Scandinavian Society for Electron Microscopy,
Atema Instruments (representing Gatan, Inc.),
Pfeiffer (representing BAL-TEC and Balzers),
E.A. Fischione Instruments, Inc.
Technoorg Linda Ltd. Co
Link Nordiska AB,
Diatome Ltd.,
Micrion,
Philips,
RMC,
Leica,
FEI.

'Beamer' - an Informal Gathering of 'Electron-Beam Workers'

For further information on this workshop, please contact the organizers:
Lynnette Madsen Lars Hultman Dept. of Physics (IFM)
e-mail: lyn-at-ifm.liu.se lhn-at-ifm.liu.se Link|ping University
voice: +46-13-284479 +46-13-281284 S-581 83 Link|ping, SWEDEN
(English) (Swedish or English) fax: +46-13-137568

http://www.ifm.liu.se/Thinfilm/html/news.html#wshop
Demonstrations and Hands-on Laboratory Sessions
(will occupy 50% of scheduled workshop time)
Use of low angle ion beam milling equipment
Mechanical preparation methods
Microtomy for materials science
Chemical Methods
Polishing
Cleaving
Replication
Includes access to TEM for examination of prepared specimens

Our Equipment
o precision low-angle ion miller (BAL-TEC RES 010)
ion miller with cooling (Gatan Duo Mill Model 600),
polishing wheels (e.g. Buehler ECOMET 4)
tripod polisher (South Bay Technology Model 590)
cleaving equipment (incl. Fischione hand disc grinder 660).
dimpler (Fischione 2000),
wire saw (South Bay Technology),
standard diamond saw,
ultrasonic disc cutter (Gatan 601)
plus other standard and older pieces of equipment.
o high-resolution 200 kV microscope with point-to-point resolution of 1.9 ]
(Philips CM20 Ultra-Twin) with EDS analytical facility,
120 kV Philips EM400 T microscope 45o tilting facilities
o Additional equipment, e.g. ultramicrotomes, will be available for the duration of
the workshop to facilitate learning a wider repertoire of techniques.

Laboratory Visits on the 28th (optional)
Several institutions in Sweden are involved with electron beam techniques. We
encourage all participants to make the most of their trip to Sweden and invite them
to visit Sweden's facilities.
Industriellt Mikroelektronik Centrum AB , (IMC), Link|ping
Ulf Wahlstr|m +46-13-281299 or fax+46-13-282200
Chalmers University, Physics Department
Dr. Eva Olsson +46-31-7723316 or fax +46-31-7723224
Lund University, Chemical Centre, Inorganic Chemistry,
Dr. L. Reine Wallenberg +46-46-108233 or fax +46-46-104525
Uppsala University, Department of Materials Science,
Dr. Stefan Johansson, +46-18-183086 or fax +46-18-555095

Second and final announcement and Preliminary program January 1997

Language of the workshop is English. Number of registrants will be
limited.

Accommodation Prices quoted are approximate for a single/double SEK per night,
* indicates special prices for the workshop if you identify yourself at the time of
booking as a participant. Please make your own reservations.
Valla Folkh|gskola (+46-13-146860 voice) on campus at 340/500;
Park Hotel* (+46-13 -129005 voice or 46-13-100418 fax) near the train station at
700/800,
Hotel du Nord* (+46-13-129895 voice or 145291 fax) near the train station at
555/650,
Good Evening Hotel (+46-13-129000 voice or 46-13-138850 fax) downtown at 555/666.
If you need more information please do not hesitate to contact us.

Telephoning: Dial 08-rest of number in Sweden, 46-8-rest of number outside of
Sweden
Hotel prices are not guaranteed. Please make your own reservations.
Buses run from Arlanda to the train station during the daytime and early evening.
A train can be taken from Stockholm to Link|ping (~2.5 h).
See reverse side for abstract format

I wish to register for the Main Programme SEK 2000 o
Beamer only SEK 500 o
cost of beamer is included for all main programme registrants
Equipment Exhibitor Fees:
} 1000 employees worldwide US$ 1000 or SEK 6000 o
{1000 employees worldwide US$ 500 or SEK 3000 o
includes entrance to conference and beamer for up to 3 persons,
and a one page advertisement in the workshop report
I wish go on the tour of Gamla Link|ping (outdoor museum of historical interest,
cost included in workshop fee) o

I would like to give a oral seminar / presentation o
present a poster o
assist with hands-on laboratory sessions o
participate in the equipment exhibit o
ASSIST IN ANY WAY NEEDED o
Reduced fees apply to persons helping with the conference.

Deadline: January 10, 1997; limited entries may be accepted up to February 20, 1997
Surname: ___________________________________________
First Name: ___________________________________________
Title: ___________________________________________
Institution: ___________________________________________
Telephone : ___________________________________________
Fax : ___________________________________________
e-mail: ___________________________________________
Address: ___________________________________________
___________________________________________
___________________________________________
if applicable, title of presentation:
________________________________________________________
________________________________________________________
Indicate whether you prefer a poster or oral presentation:
poster- 1 board o 2 boards o oral- desired duration ____ minutes
Is this original or recent research? _____
Do you plan on submitting a full length manuscript? ______

Indicate the laboratory skills you would like to learn
(number in order of importance)
Mechanical preparation methods incl. ion beam milling ______
Polishing ______ Cleaving ______
Use of low angle ion beam milling equipment ______
Microtomy for materials science ______
Chemical Methods (e.g. MgO thinning) / Carbon Replica ______

Are you interested in sessions on:
How to choose your techniques? ________
How to set-up your laboratory? ________

Call for Papers
o original papers can often be accommodated in either an oral or poster format
according to the authors wishes (time permitting):
o posters of an instructional nature previously displayed are also welcome; original
presentation site to be included on the display and in workshop report. Poster
boards 118 cm by 118 cm in size provided. 1 or 2 boards may be used, however, note
that a fold in the boards occurs at the dividing point in each pair.
o Abstracts (regular or extended) should be submitted by the above deadline. A
format is suggested on page 4. Please provide the fax number and e-mail address (if
possible) of the person presenting the paper. 3 cm margins are recommended.

Travel
Within Sweden: by train or car

From farther away:
o Link|ping has a small airport (code JZ).
o It may be more convenient to fly into Norrk|ping (with connections to Copenhagen
and Stockholm) and take a taxi to Link|ping (for a cost of approximately ~300 SEK
and best arranged in advance: voice +46-13-160939, fax -163151).
o If you fly into Arlanda-Stockholm airport then you may need to stay overnight:

Arlanda
Good Morning Hotel, Box 51, 190 45 Stockholm-Arlanda,
telephone +46-8-65501000, Single room ~695:- / Double room ~795:- SEK
Arlandia Hotel SAS, Arlanda Flygplats, Box 103, 190 45 Stockholm-Arlanda,
phone +46-8-59361800, fax +46-8-59361970, Single room ~1250:- /Double ~1450:- SEK

Stockholm (near train station)
Prize Hotel, Kungsbron 1, Stockholm, telephone +46-8-149450, fax +46-8-149848
Single room ~800:- / Double room ~985:- SEK,
Hotel Adlon, Vasagatan 42, 111 20 Stockholm, telephone +46-8-245400, fax
+46-8-208610
Single room ~715 to 895:- / Double room ~1050:- SEK
Stockholm Central Hotel AB, Vasagatan 38, S-111 20 Stockholm Sweden, phone +46-8-22
08 40, telefax +46-8-24 75 73, single room ~650 to 1075:- / double room ~650 to
1275:- SEK

Format for abstracts (title and names, affliation centered; abstract left-justified)

TEM Sample Preparation of Material Y by Technique Z

Jane E. Smith
Company ABC, Inc., Address

John F. Doe
ABC University, Address
Fax: 12-344556, e-mail: doe-at-abc.unive.nw

Copies of the abstracts will be made available at the workshop in the form of a
Link|ping University report which can be referred to.
Full articles associated with the conference will be gathered for a special issue
of the fully refereed journal, Micron in the Jan.-Feb. 1998 issue. Manuscripts
should be submitted at the workshop. The regular review procedure will be followed.

_____________________
Merry Christmas and a Happy New Year


Lynnette

_____________________________________________________________
Dr.L.D.Madsen lynma-at-ifm.liu.se FAX: (0)13-137568
http://www.ifm.liu.se/Thinfilm/

Institutionen f|r Fysik och M{tteknik (IFM), Fysikhuset
Link|pings Universitet
581 83 Link|ping, SVERIGE 013-284479

Department of Physics (IFM), F-house
Linkoping University '!/
S-581 83 Linkoping, SWEDEN +46 13 284479 -at- -at- +----------------------------------------oOO-(_)-OOo---------+




From: Alan Brooker :      alanb-at-jeolsys.demon.co.uk
Date: Mon, 23 Dec 1996 09:40:57 0000
Subject: unsubscribe

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unsubscribe alanb-at-jeolsys.demon.co.uk
*---------------------------------------*----------------------------------*
| Dr.Alan Brooker | Email: alanb-at-jeolsys.demon.co.uk |
| Product Support Manager | |
| JEOL (UK) Ltd., JEOL House, | Tel: +44 (0) 707 377117 |
| Watchmead, Welwyn Garden City, | Fax: +44 (0) 707 373254 |
| Herts, AL7 1LT, United Kingdom | |
*---------------------------------------*----------------------------------*





From: Ming Chen :      mingchen-at-gpu.srv.ualberta.ca
Date: Mon, 23 Dec 1996 09:45:44 -0700 (MST)
Subject: Re: image capture from SEM

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On Tue, 17 Dec 1996 wise-at-vaxa.cis.uwosh.edu wrote:

} ------------------------------------------------------------------------
} The Microscopy ListServer -- Sponsor: The Microscopy Society of America
} To Subscribe/Unsubscribe -- Send Email to ListServer-at-MSA.Microscopy.Com
} -----------------------------------------------------------------------.
I had a demostration of PCI image system from Hitachi at my E.M. Unit a
year ago. I was quiet impressed about how easy to capture the image from
my Hitachi S-2500 SEM and replay back to camera for photo quality picture.
Now acturally I just purchased the sytem a week ago. I played around a
bit and found it is even better than what I thought. In the version 4 of
the system , it is more easy to get 3-D image and color the images
capatured from SEM. I plan to introduce it to my clients to use it as
daily research and get instant images on printer or store in a zip drive
100 MB disk in the new year.

Merry Christmas and a Happy New Year !!!



} To all, }
} Portions of this subject have been discussed over the past few
} months but I haven't really been paying close attention to it. I apologize
} in advance if we rehash ground already covered.
} We have a (an?) Hitachi 2460N SEM and I'm looking into outfitting
} it for digital image capture (with annotation, file storage, printer, etc).
} I know that Hitachi sells a system that is supposed to plug right in and
} comes with a lot of bells and whistles. Has anyone had any experience with
} the Hitachi system? Alternatively, does anyone know of an after market
} product that performs well? Given that I am a computer dummy, I would be
} more inclined to purchase a total system rather than attempt to put one
} together on my own from individual components.
} Please respond directly to me and not to the list.
}
} Thanks in advance,
}
} Bob
}
}
} Robert R. Wise, PhD
} Director, UWO Electron Microscope Facility
} Department of Biology
} University of Wisconsin Oshkosh
} Oshkosh, WI 54901
} (414) 424-3404 tel
} (414) 424-1101 fax
} wise-at-uwosh.edu
}
}
}


***********************************************
* Ming H. Chen, PhD *
* Medicine/Dentistry Electron Microscopy Unit *
* University Of Alberta. *
* Edmonton, Alberta, Canada *
***********************************************








From: Dr. H. Adelmann :      106421.3362-at-compuserve.com
Date: Mon, 23 Dec 1996 06:56:32 -0500
Subject: Taricha Lung Cells

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Hello all,

I have to generate video files from lung cell division of Taricha granulosa
(newt) in phase, DIC and fluorescence. Is anybody out there - preferably in
Germany, but may be anywhere on this small planet - that could provide me
with stock material of this cell line to get it cultured ?

Thanks

Holger G. Adelmann
{106421.3362-at-compuserve.com}




From: Dr. H. Adelmann :      106421.3362-at-compuserve.com
Date: Mon, 23 Dec 1996 07:00:27 -0500
Subject: Roto-Compressor

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Hello all,

I'm looking for a device that is called Rotocompressor. It fits onto the
microscope stage and was originally constructed by American
protozoologists. It could be used to observe protists and cells in general
while rotating them to any position wanted and applying defined pressure to
them in order to get them flattened. Is anybody aware of such a device and
a manufacturer ?

Thanks

Holger G. Adelmann
{106421.3362-at-compuserve.com}




From: moxtek-at-moxtek.win.net (Clark Turner)
Date: 18 Dec 96
Subject: EDS deconvolution algorithms (fwd)

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X-Mailer: WinNET Mail, v2.51
Message-ID: {143-at-moxtek.win.net}
Reply-To: moxtek-at-MOXTEK.WIN.NET (Clark Turner)
To: microscopy-at-Sparc5.Microscopy.Com

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FORWARDED MAIL -------

Dear Microscopy List -

We are interested in doing peak deconvolution of an x-ray
spectrum. Our instrument has a routine that performs automatic
background subtraction and then a least squares minimization using
standard files to deconvolute the unknown peaks in the spectrum.
However, the method is limited to a maximum of 15 elements. To
automate the method for unknown analysis we would like to have at
least 30 elements, and perhaps as many as 40. Of course, all
available x-ray lines from 1 keV to 30 keV will be of interest.

Does anyone know of available computer programs to perform this
data reduction? We would like something in the public domain, but
would be willing to buy a commercial package if necessary. We
would also welcome any recommendations from the literature.

Thank you in advance for any input you can give us.

D. Clark Turner
MOXTEK, Inc.
452 West 1260 North
Orem, Utah 84057

email moxtek-at-moxtek.win.net
phone (801) 225-0930







From: Sara Miller :      saram-at-acpub.duke.edu
Date: Mon, 23 Dec 1996 10:36:15 -0500 (EST)
Subject: Re: Asbestos: tremolite/chrysotile ratio determination

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On Tue, 26 Nov 1996, Goran Drazic wrote:

} Date: Tue, 26 Nov 1996 15:30:21 +0100
} From: Goran Drazic {goran.drazic-at-ijs.si}
} To: Microscopy-at-Sparc5.Microscopy.Com
} Subject: Asbestos: tremolite/chrysotile ratio determination
}
}
} Dear Microscopists,
}
} I am looking for a (practical) method or procedure for the determination
} of tremolite/ chrysotile ratio (volume, mass, number of fibers) in bulk
} samples (asbestos-cement products). I will be very grateful if you are
} willing to share any experience regarding this matter using optical M,
} SEM, TEM, XRD or classical analytical chemistry.
}
} Best regards,
}
}
} Dr. Goran Drazic
} Ceramics department
} J. Stefan Institute, University of Ljubljana
} Slovenia
}
Try contacting Dr. Victor Roggli, Pathology, Box 3712 Duke Med Ctr, Durham,
NC 27710; 919 286 0411 X6615. (Expert on asbestos).Not sure of email
address. Try roggli.victor-at-forum.va.gov or maybe
roggli-at-forum.va.gov

Sara E. Miller, Ph. D.
P. O. Box 3020
Duke University Medical Center
Durham, NC 27710
Ph: 919 684-3452
FAX: 919 684-8735








From: Tang Ee Koon :      medlab2-at-leonis.nus.sg
Date: Fri, 27 Dec 1996 14:12:08 +0800 (SST)
Subject: Subscribe

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Please subscribe the following :
medlab2-at-leonis.nus.sg


Thank you very much.






From: Tang Ee Koon :      medlab2-at-leonis.nus.sg
Date: Fri, 27 Dec 1996 14:16:39 +0800 (SST)
Subject: Subscribe

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Sorry for the previous subscription mail sent to the wrong e-mail address.

Regards





From: Mohan Kalyanaraman :      mxkalyan-at-pau.mobil.com
Date: Fri, 27 Dec 96 12:58:26 EST
Subject: Thank you for AC noise response

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A belated thank you to everyone who responded regarding AC noise
question. As many of you suggested the problem was caused by
vibration. We worked with our Philips engineer to deduce the source of
the problem.

The column is on a slab and one of the steel bars holding the floor
was making contact with the slab and this transmitted the vibration.
We had to take the floor boards apart and it was easy to see where it
was touching. Now the scope is doing much better. If anyone would like
a summary of responses, please e-mail me.

Thank you and have a good new year.

Mohan Kalyanaraman
Mobil Technology Company
PO Box 480
Paulsboro, NJ 08066
609-224-3989
mxkalyan-at-pau.mobil.com





From: jkw1-at-axe.humboldt.edu (John K. Weaver)
Date: Sat, 28 Dec 1996 15:34:35 -0800
Subject: Zeiss Epi-tube for sale

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I have a Zeiss Epifluorescent Illumination condenser tube with the
filter/beam spliter for sale. It is for the 160 mm tube length scopes,
pictures are available for downloading upon request from serious buyers.
Excellent condition, does not include reflector housing or lamp. Currently
priced in the US at over $3200.00. Will sell for $2000.00 US. Pursuing a
masters degree and have references.

Mr. John K. Weaver 707 839-5651 ,California, USA
jkw1-at-axe.humboldt.edu
John K. Weaver, jkw-at-axe.humboldt.edu
Department of Biological Sciences
Humboldt State University, Arcata, CA





From: Lauri J. Pelliniemi :      ljpelmi-at-utu.fi
Date: Mon, 30 Dec 1996 10:49:44 -0800 (PST)
Subject: Re: DAB Precipitate

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On Wed, 18 Dec 1996, Cheri Owen wrote:
} Yuck! We have DAB precip on our immunocytochemistry sections. They are
} Araldite embed sections on slides that we have etched and circled with PAP
} pen. Has anyone else had this problem and do you have any solutions? Is
} it possible to get the precip off once it has formed? We haven't had any
} luck. Any suggestions would be greatly appreciated.
}
} Cheri Owen
} Detroit Neurotrauma Institute
} Wayne State University
} Detroit, Michigan
} (313)577-4648
}
Dear Cheri:
Do you get the precipitate if you do not use the pen?
The DAB may also contain impurities as explained in: Pelliniemi et al.,
1980 J. Histochem. Cytochem. 28:191-192. I hope this will be of help.

----------------------------------------------------------------------------
I Dr. Lauri J. Pelliniemi Telephone +358-2-333 7312 I
I Associate Professor or +358-2-333 7209 I
I Laboratory of Electron Microscopy I
I University of Turku Telefax +358-2-333 7380 I
I Kiinamyllynkatu 10 Internet Lauri.Pelliniemi-at-utu.fi I
I FIN-20520 Turku http://www.utu.fi/research/crede/pelliniemi.html I
I FINLAND, Europe http://www.utu.fi/med/em/index.html I
----------------------------------------------------------------------------





From: Gary H. Zajic :      zajic-at-umich.edu
Date: Mon, 30 Dec 1996 16:50:12 -0500 (EST)
Subject: 3D image registration help

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Hello Fellow Microscopists,
I have made serial sections of guinea pig inner ear and need some
help at this to register the sections in order to make a 3D
reconstruction. The sections are approx 0.7 micron, stained
and photographed with a digital camera. There are fifty images
stored in tif format each being 4.6 megs so I cannot include
examples of these images to the entire newsgroup.
The sections were cut from tissue (normal control) used in another
experiment and are no way involved with my research commitments to
the lab. It is an "after hours" personal project I have been meaning
to finish but cannot find the time just now to handle each of
the sections. If anyone would be interested in helping me to
stack these images I think we could make a quite nice reconstruction.
and would be glad to discuss it with you.
I hope you all have a really Happy New Year!
Sincerely,

Gary Zajic
Kresge Hearing Research Institute
Biochemistry Laboratory
1301 E Ann
Ann Arbor, MI 48109
zajic-at-umich.edu





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